title: Performance of the Roche LightCycler real-time PCR assay for diagnosing extrapulmonary tuberculosis.
abstract: The Roche LightCycler mycobacterium detection molecular assay for Mycobacterium tuberculosis, M. avium, and M. kansasii, was applied to tissue specimens. It performed well on lymph node and cerebrospinal fluid specimens and less well on lung, liver, and bone marrow core biopsy specimens, but used in conjunction with a clinical suspicion of tuberculosis, it could augment patient management.
authors.affiliation: Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. natasha.gous@gmail.com
authors.forename: N
authors.initials: N
authors.lastname: Gous
authors.forename: L E
authors.initials: LE
authors.lastname: Scott
authors.forename: E
authors.initials: E
authors.lastname: Wong
authors.forename: T
authors.initials: T
authors.lastname: Omar
authors.forename: W D F
authors.initials: WD
authors.lastname: Venter
authors.forename: W
authors.initials: W
authors.lastname: Stevens
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques / Q000379:methods*; D001853:Bone Marrow / Q000382:microbiology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D005260:Female; D006801:Humans; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology*