title: [Determination of hydroxyproline in rat kidney tissue by HPLC with pre-column derivatization].
abstract: OBJECTIVE
  To establish a pre-column derivatization HPLC method for quantitation of hydroxyproline (Hyp) in rat kidney tissue by reversed-phase assay.
  
  
  METHODS
  Rat kidney samples were hydrolyzed in 6 mol x L⁻¹ HCl under 110 degree for 24 h, then 9-fluorenylmethyl chloroformate (FMOC) was added for the pre-column reaction. The HPLC analysis was performed on a Phenomenex C₁₈ column using sodium acetate buffer, methanol and acetonitrile as mobile phase with a flow rate of 1.0 ml min⁻¹ and UV detective wavelength 265 nm at 40 degree.
  
  
  RESULTS
  Linear range was 15.30-612.00 mg x L⁻¹ (correlation coefficient was 0.9999). Recovery rate was 97.4%-103.9%.
  
  
  CONCLUSIONS
  The established method is simple, accurate and sensitive to analyze Hyp content in rat kidney tissue.
authors.affiliation: Institute of Materia Medica, Zhejiang University City College, Hangzhou 310015, China.
authors.forename: Er-gang
authors.initials: EG
authors.lastname: Liu
authors.forename: Xu-chun
authors.initials: XC
authors.lastname: Fu
authors.forename: Ying
authors.initials: Y
authors.lastname: Qian
authors.forename: Hai-bo
authors.initials: HB
authors.lastname: Bai
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006909:Hydroxyproline / Q000032:analysis*; D066298:In Vitro Techniques; D007668:Kidney / Q000737:chemistry*; D008297:Male; D051381:Rats
chemical_list: D006909:Hydroxyproline