title: Expression of TMEM166 protein in human normal and tumor tissues.
abstract: Transmembrane protein 166 (TMEM166) is a novel human regulator involved in both autophagy and apoptosis. In this study, we generated a specific rabbit polyclonal antibody against human TMEM166 and assessed the expression of this protein in various human normal and tumor tissue samples by tissue microarray-based immunohistochemical analysis. Varying TMEM166 protein levels were expressed in a cell-type and tissue-type-specific manner in detected tissues or organs. Strong TMEM166 expression was shown in the glomerular zona of the adrenal cortex, chromophil cells of the pituitary gland, islet cells, squamous epithelium of the esophagus mucosa, the fundic gland, and hepatocytes. Moderate or weak TMEM166 staining was identified in the parathyroid gland, the testis, vaginal stratified squamous cells, lung macrophages, hematopoietic cells, renal tubular epithelial cells, macrophages in the spleen red pulp, and neuronal cells in the cerebral cortex. Some tissues failed to stain for TMEM166, such as adipose tissue, colon, cerebellum, lymph node, mammary gland, ovary, prostate, rectum, skin, small intestine, thyroid gland, tonsil, and thymus. In comparing human normal and tumor tissues, TMEM166 expression was widely downregulated in the cancer tissues. Our studies provide the basis for future investigations into cell-type-specific functions of this protein in human normal and tumor tissues.
authors.affiliation: *Key Laboratory of Medical Immunology, Ministry of Health †Peking University Center for Human Disease Genomics ‡Department of Thoracic Surgery, Peking University People's Hospital §Department of Pathology, School of Basic Medical Sciences, Peking University, Beijing, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Xu
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Huiying
authors.initials: H
authors.lastname: He
authors.forename: Jia
authors.initials: J
authors.lastname: Hu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Lv
authors.forename: Dalong
authors.initials: D
authors.lastname: Ma
authors.forename: Ying Yu
authors.initials: YY
authors.lastname: Chen
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D046888:Tissue Array Analysis
chemical_list: D000906:Antibodies; C524094:EVA1A protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger