title: Age- and gender-related alteration in plasma advanced oxidation protein products (AOPP) and glycosaminoglycan (GAG) concentrations in physiological ageing.
abstract: BACKGROUND
  The authors studied the role of increased oxidative stress in the development of oxidative protein damage and extracellular matrix (ECM) components in ageing. The age- and gender-associated disturbances in connective tissue metabolism were evaluated by the plasma chondroitin sulphated glycosaminoglycans (CS-GAG) and non-sulphated GAG-hyaluronan (HA) measurements. Plasma concentration of advanced oxidation protein products (AOPP) was analysed in order to assess oxidative protein damage and evaluate the possible deleterious role of oxidative phenomenon on tissue proteoglycans' metabolism during the physiological ageing process.
  
  
  METHODS
  Sulphated and non-sulphated GAGs as well as AOPP were quantified in plasma samples from 177 healthy volunteers.
  
  
  RESULTS
  A linear age-related decline of plasma CS-GAG level was found in this study (r=-0.46; p<0.05). In contrast, HA concentrations rise gradually with age (r=0.44; p<0.05) in plasma samples. For both ECM components, the observed differences were not gender-specific. A strong age-dependent relationship has been shown in regard to AOPP. AOPP levels significantly increased with age (r=0.63; p<0.05), equally strongly in both men (r=0.69; p<0.05) and women (r=0.57; p<0.05) during physiological ageing. A significant correlation was found between the concentrations of AOPP and both CS-GAG (r=-0.31; p<0.05) and HA (r=0.33; p<0.05).
  
  
  CONCLUSIONS
  Proceeding with age changes in the ECM are reflected by CS-GAG and HA plasma levels. Strong correlations between AOPP and ECM components indicate that oxidative stress targets protein and non-protein components of the connective tissue matrix during human ageing.
authors.affiliation: Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia, Sosnowiec, Poland. kvassev@sum.edu.pl
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Komosinska-Vassev
authors.forename: Pawel
authors.initials: P
authors.lastname: Olczyk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Winsz-Szczotka
authors.forename: Kornelia
authors.initials: K
authors.lastname: Kuznik-Trocha
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Klimek
authors.forename: Krystyna
authors.initials: K
authors.lastname: Olczyk
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood* / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006025:Glycosaminoglycans / Q000097:blood*; D006801:Humans; D006820:Hyaluronic Acid / Q000097:blood; D007223:Infant; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011509:Proteoglycans / Q000097:blood; D012727:Sex Characteristics; D055815:Young Adult
chemical_list: D001798:Blood Proteins; D006025:Glycosaminoglycans; D011509:Proteoglycans; D006820:Hyaluronic Acid