title: [Experimental study on the glutamine's intervention effect on the opening of permeability transition pore in myocardial mitochondrial membrane].
abstract: OBJECTIVE
  To explore the intervention effect and the possibly mechanism of the glutamine (Gln) on the opening change of the permeability transition pore (PTP) in the myocardial mitochondrial membrane under the overtraining state.
  
  
  METHODS
  30 SD rats were randomly divided into 3 groups (n = 10): control group (CG group), overtraining group (OG group) and supplementary (Gln) + overtraining group group). Spectrophotometry was used to test the openness of the permeability transition pore in the myocardial mitochondrial membrane. Electrochemistry was used to test the malondialdehyde (MDA) and the glutathione (GSH) content and the phospholipase A2 (PLA2) activity.
  
  
  RESULTS
  OG group compared with the GOG group, the absorbance (A0) and the absorbance change (Delta A) were decreased significantly (P < 0.05). Rh123 fluorescence (F0) intensity was significantly increased (P < 0.05). Rhodamine123 (Rh123) fluorescence change (delta F) was significantly decreased (P < 0.05). Compared with the GOG, the mitochondrial GSH was significantly decreased (P < 0.05), the PLA2 activity and the content of MDA were significantly increased (P <0.05).
  
  
  CONCLUSIONS
  Overtraining could lead to opening increase of permeability transition pore in the myocardial mitochondrial membrane, after overtraining, the production of the reactive oxygen species (ROS) and PLA2 activity were increased, GSH content was decreased. But added exogenous Gln had a significant intervention effect for these changes.
authors.affiliation: College of Physical Education, Liaocheng Normal University, Liaocheng, China. liutiemin@lcu.edu.cn
authors.forename: Tie-Min
authors.initials: TM
authors.lastname: Liu
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005973:Glutamine / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000502:physiology; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000187:drug effects* / Q000502:physiology*; D000083162:Mitochondrial Permeability Transition Pore; D009206:Myocardium / Q000378:metabolism; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D017382:Reactive Oxygen Species; D005973:Glutamine; D005978:Glutathione