title: Physically crosslinked nanocomposites from silicate-crosslinked PEO: mechanical properties and osteogenic differentiation of human mesenchymal stem cells.
abstract: The mechanical and biological properties of silicate-crosslinked PEO nanocomposites are studied. A strong correlation is observed between silicate concentration and mechanical properties. In vitro cell culture studies reveal that an increase in silicate concentration enhances the attachment and proliferation of human mesenchymal stem cells significantly. An upregulation in the expression of osteocalcin on nanocomposites compared to the tissue culture polystyrene control is observed. Together, these results suggest that silicate-based nanocomposites are bioactive and have the potential to be used in a range of biotechnological and biomedical applications such as injectable matrices, biomedical coatings, drug delivery, and regenerative medicine.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907-2032, USA. agaharwa@purdue.edu
authors.forename: Akhilesh K
authors.initials: AK
authors.lastname: Gaharwar
authors.forename: Vipuil
authors.initials: V
authors.lastname: Kishore
authors.forename: Christian
authors.initials: C
authors.lastname: Rivera
authors.forename: Whitney
authors.initials: W
authors.lastname: Bullock
authors.forename: Chia-Jung
authors.initials: CJ
authors.lastname: Wu
authors.forename: Ozan
authors.initials: O
authors.lastname: Akkus
authors.forename: Gudrun
authors.initials: G
authors.lastname: Schmidt
mesh_terms: D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D016503:Drug Delivery Systems / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D053761:Nanocomposites / Q000737:chemistry*; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis; D011137:Polystyrenes / Q000737:chemistry; D017640:Silicates / Q000737:chemistry*
chemical_list: D011137:Polystyrenes; D017640:Silicates; D015675:Osteocalcin