title: Effects of MMP inhibitors incorporated within dental adhesives.
abstract: Matrix metalloproteinase (MMP) inhibition has been shown to reduce adhesive bond degradation when applied as a pre-conditioner, adding to clinical steps in the placement of adhesives, but their incorporation within dental adhesives has not been fully explored. This study examined the effect of including 2 MMP inhibitors (BB94 and GM6001) within the primers of 3 commercially available adhesives. Fluorometric assay and zymography showed that adhesives with MMP inhibitors had high affinity toward both synthetic fluorogenic FRET peptides (95%) and dentin powder substrates, respectively. The immediate microtensile bond strength was enhanced for 2 types of adhesives following the addition of both inhibitors. However, no changes were detected between the control and the inhibitor groups following 3-month storage. The modified two-step etch-and-rinse and single-step systems showed less Rhodamine B penetration to the "hybrid layer" and to the "adhesive", respectively. The incorporation of BB94 and GM6001 within the primers resulted in the inhibition of dentin MMPs with improved initial bond strength and enhanced sealing ability.
authors.affiliation: Biomaterials, Biomimetics & Biophotonics Research Group, King's College London Dental Institute at Guy's Hospital, King's Health Partners, London, UK.
authors.forename: A
authors.initials: A
authors.lastname: Almahdy
authors.forename: G
authors.initials: G
authors.lastname: Koller
authors.forename: S
authors.initials: S
authors.lastname: Sauro
authors.forename: J W
authors.initials: JW
authors.lastname: Bartsch
authors.forename: M
authors.initials: M
authors.lastname: Sherriff
authors.forename: T F
authors.initials: TF
authors.lastname: Watson
authors.forename: A
authors.initials: A
authors.lastname: Banerjee
mesh_terms: D000293:Adolescent; D000328:Adult; D001840:Dental Bonding; D003763:Dental Leakage / Q000517:prevention & control*; D003799:Dental Stress Analysis; D003804:Dentin / Q000201:enzymology; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004151:Dipeptides; D006801:Humans; D016015:Logistic Models; D008422:Materials Testing; D061965:Matrix Metalloproteinase Inhibitors; D008689:Methacrylates / Q000737:chemistry; D008963:Molar; D010649:Phenylalanine / Q000031:analogs & derivatives; D011109:Polymethacrylic Acids / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry*; D013718:Tensile Strength; D013876:Thiophenes; D055815:Young Adult
chemical_list: D017220:Dentin-Bonding Agents; D004151:Dipeptides; C498353:G-Bond; D061965:Matrix Metalloproteinase Inhibitors; D008689:Methacrylates; C078131:N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide; C469607:OptiBond FL; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; D013876:Thiophenes; D010649:Phenylalanine; C080985:batimastat