title: Micro-Raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells.
abstract: We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 µg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 µg/ml.
authors.affiliation: Centre for Atomic and Molecular Physics, Manipal University, Manipal, India.
authors.forename: Aseefhali
authors.initials: A
authors.lastname: Bankapur
authors.forename: R Sagar
authors.initials: RS
authors.lastname: Krishnamurthy
authors.forename: Elsa
authors.initials: E
authors.lastname: Zachariah
authors.forename: Chidangil
authors.initials: C
authors.lastname: Santhosh
authors.forename: Basavaraj
authors.initials: B
authors.lastname: Chougule
authors.forename: Bhavishna
authors.initials: B
authors.lastname: Praveen
authors.forename: Manna
authors.initials: M
authors.lastname: Valiathan
authors.forename: Deepak
authors.initials: D
authors.lastname: Mathur
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000009:adverse effects*; D052898:Optical Tweezers; D018384:Oxidative Stress / Q000187:drug effects; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D012834:Silver