title: Comparative proteomic analysis of primary schwann cells and a spontaneously immortalized schwann cell line RSC 96: a comprehensive overview with a focus on cell adhesion and migration related proteins.
abstract: Schwann cells (SCs) are the principal glial cells of the peripheral nervous system (PNS). As a result of tissue heterogeneity and difficulties in the isolation and culture of primary SCs, a considerable understanding of SC biology is obtained from SC lines. However, the differences between the primary SCs and SC lines remain uncertain. In the present study, quantitative proteomic analysis based on isobaric tags for relative and absolute quantitation (iTRAQ) labeling was conducted to obtain an unbiased view of the proteomic profiles of primary rat SCs and RSC96, a spontaneously immortalized rat SC line. Out of 1757 identified proteins (FDR < 1%), 1702 were quantified, while 61 and 78 were found to be, respectively, up- or down-regulated (90% confidence interval) in RSC96. Bioinformatics analysis indicated the unique features of spontaneous immortalization, illustrated the dedifferentiated state of RSC96, and highlighted a panel of novel proteins associated with cell adhesion and migration including CADM4, FERMT2, and MCAM. Selected proteomic data and the requirement of these novel proteins in SC adhesion and migration were properly validated. Taken together, our data collectively revealed proteome differences between primary SCs and RSC96, validated several differentially expressed proteins with potential biological significance, and generated a database that may serve as a useful resource for studies of SC biology and pathology.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University , 19 Qixiu Road, Nantong, JS 226001, P. R. China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Ji
authors.forename: Mi
authors.initials: M
authors.lastname: Shen
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Shuqiang
authors.initials: S
authors.lastname: Zhang
authors.forename: Shu
authors.initials: S
authors.lastname: Yu
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.forename: Fei
authors.initials: F
authors.lastname: Ding
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D002469:Cell Separation; D015536:Down-Regulation; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D061251:Primary Cell Culture; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D009419:Nerve Tissue Proteins; D020543:Proteome