title: Gene suppression of mouse testis in vivo using small interfering RNA derived from plasmid vectors.
abstract: We evaluated whether inhibiting gene expression by small interfering RNA (siRNA) can be used for an in vivo model using a germ cell-specific gene (Tex101) as a model target in mouse testis. We generated plasmid-based expression vectors of siRNA targeting the Tex101 gene and transfected them into postnatal day 10 mouse testes by in vivo electroporation. After optimizing the electroporation conditions using a vector transfected into the mouse testis, a combination of high- and low-voltage pulses showed excellent transfection efficiency for the vectors with minimal tissue damage, but gene suppression was transient. Gene suppression by in vivo electroporation may be helpful as an alternative approach when designing experiments to unravel the basic role of testicular molecules.
authors.affiliation: Department of Molecular Medicine and Anatomy, Nippon Medical School, Tokyo, Japan.
authors.forename: Takami
authors.initials: T
authors.lastname: Takizawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Takuji
authors.initials: T
authors.lastname: Kosuge
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Mizuguchi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Sato
authors.forename: Takehiko
authors.initials: T
authors.lastname: Koji
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Araki
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Takizawa