title: Complete sequence analysis of two methanotroph-specific repABC-containing plasmids from Methylocystis sp. strain SC2.
abstract: The complete nucleotide sequences of two large, low-copy-number plasmids of 229.6 kb (pBSC2-1) and 143.5 kb (pBSC2-2) were determined during assembly of the whole-genome shotgun sequences of the methane-oxidizing bacterium Methylocystis sp. strain SC2. The physical existence of the two plasmids in strain SC2 was confirmed by pulsed-field gel electrophoresis followed by Southern hybridization. Both plasmids have a conserved replication module of the repABC system and carry genes involved in their faithful maintenance and conjugation. In addition, they contain genes that might be involved in essential metabolic processes. These include several heavy metal resistance genes and copper transport genes in pBSC2-1 and a complete nitrous oxide reductase operon and a pmoC singleton in pBSC2-2, the latter encoding the PmoC subunit of particulate methane monooxygenase.
authors.affiliation: Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
authors.forename: Bomba
authors.initials: B
authors.lastname: Dam
authors.forename: Michael
authors.initials: M
authors.lastname: Kube
authors.forename: Somasri
authors.initials: S
authors.lastname: Dam
authors.forename: Richard
authors.initials: R
authors.lastname: Reinhardt
authors.forename: Werner
authors.initials: W
authors.lastname: Liesack
mesh_terms: D015139:Blotting, Southern; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D053858:Metabolic Networks and Pathways / Q000235:genetics; D042083:Methylocystaceae / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial