title: Development of a near-infrared fluorescence ELISA method using tyramide signal amplification.
abstract: In this study, we applied tyramide signal amplification (TSA) to fluorescence enzyme-linked immunosorbent assay (ELISA) employing horseradish peroxidase (HRP) as the detection enzyme. When used with a human epidermal growth factor ELISA kit, the TSA method led to a >100-fold increase in fluorescence signal intensity in comparison to an unamplified method. It also showed wider dynamic range and better sensitivity compared to a conventional method using tetramethylbenzidine as the HRP substrate.
authors.affiliation: LI-COR Biosciences, Lincoln, NE 68504, USA. herbert.gong@licor.com
authors.forename: Haibiao
authors.initials: H
authors.lastname: Gong
authors.forename: Mark
authors.initials: M
authors.lastname: Cradduck
authors.forename: Lael
authors.initials: L
authors.lastname: Cheung
authors.forename: D Michael
authors.initials: DM
authors.lastname: Olive
mesh_terms: D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000032:analysis; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007259:Infrared Rays; D013379:Substrate Specificity; D014439:Tyramine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D007074:Immunoglobulin G; C111551:biotinyltyramide; D004815:Epidermal Growth Factor; D001710:Biotin; D006735:Horseradish Peroxidase; D014439:Tyramine