title: [Separation and morphological observation of mouse testis seminiferous tubule epithelium].
abstract: OBJECTIVE
  To explore a simple and feasible technique to locate proteins during spermatogenesis.
  
  
  METHODS
  Various germ cells and somatic cells were separated by collagenase I and DNase I after the albuginea was removed. The cells were then smeared, dyed, and observed directly under fluorescence and confocal microscopy.
  
  
  RESULTS
  Germ cells at different steps were successfully identified by specific dyestuffs for acrosome and nucleolus.
  
  
  CONCLUSIONS
  A simple method for locating proteins during spermatogenesis was successfully developed.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
authors.forename: Shi-Ying
authors.initials: SY
authors.lastname: Miao
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D012670:Seminiferous Epithelium / Q000166:cytology*; D013094:Spermatozoa / Q000166:cytology*