title: BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder.
abstract: OBJECTIVE
  To determine whether single nucleotide polymorphisms (SNPs) of the brain-derived neurotrophic factor (BDNF) that have been associated with bipolar illness are associated with physiological dysfunction.
  
  
  METHODS
  Lymphoblastoid cell lines (n=30) obtained from bipolar I individuals carrying zero, one, or two copies of a BDNF SNP associated with bipolar illness (rs12273363) were utilized.
  
  
  RESULTS
  Proapoptotic stressors of serum deprivation alone, or serum deprivation combined with the sodium ionophore, monensin, did not alter intracellular proBDNF. Monensin treatment increased mature-BDNF (mBDNF) protein levels (P<0.05). There were no differences related to the presence of SNP or copy number.
  
  
  CONCLUSIONS
  rs12273363 does not appear to have functional consequences that would involve its role in bipolar illness.
authors.affiliation: Mood Disorder Research Program, Department of Psychiatry and Behavioral Science, School of Medicine, University of Louisville, Louisville, Kentucky 40292, USA.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Gao
authors.forename: Mathew
authors.initials: M
authors.lastname: Galante
authors.forename: James
authors.initials: J
authors.lastname: El-Mallakh
authors.forename: John I
authors.initials: JI
authors.lastname: Nurnberger
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Delamere
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Lei
authors.forename: Rif S
authors.initials: RS
authors.lastname: El-Mallakh
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001714:Bipolar Disorder / Q000235:genetics*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D053938:DNA Fragmentation; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D019208:Brain-Derived Neurotrophic Factor