title: Contrasting effects of Bifidobacterium breve NCIMB 702258 and Bifidobacterium breve DPC 6330 on the composition of murine brain fatty acids and gut microbiota.
abstract: BACKGROUND
  We previously showed that microbial metabolism in the gut influences the composition of bioactive fatty acids in host adipose tissue.
  
  
  OBJECTIVE
  This study compared the effect of dietary supplementation for 8 wk with human-derived Bifidobacterium breve strains on fat distribution and composition and the composition of the gut microbiota in mice.
  
  
  METHODS
  C57BL/6 mice (n = 8 per group) received B. breve DPC 6330 or B. breve NCIMB 702258 (10(9) microorganisms) daily for 8 wk or no supplement (controls). Tissue fatty acid composition was assessed by gas-liquid chromatography while 16S rRNA pyrosequencing was used to investigate microbiota composition.
  
  
  RESULTS
  Visceral fat mass and brain stearic acid, arachidonic acid, and DHA were higher in mice supplemented with B. breve NCIMB 702258 than in mice in the other 2 groups (P < 0.05). In addition, both B. breve DPC 6330 and B. breve NCIMB 702258 supplementation resulted in higher propionate concentrations in the cecum than did no supplementation (P < 0.05). Compositional sequencing of the gut microbiota showed a tendency for greater proportions of Clostridiaceae (25%, 12%, and 18%; P = 0.08) and lower proportions of Eubacteriaceae (3%, 12%, and 13%; P = 0.06) in mice supplemented with B. breve DPC 6330 than in mice supplemented with B. breve NCIMB 702258 and unsupplemented controls, respectively.
  
  
  CONCLUSIONS
  The response of fatty acid metabolism to administration of bifidobacteria is strain-dependent, and strain-strain differences are important factors that influence modulation of the gut microbial community by ingested microorganisms.
authors.affiliation: Alimentary Pharmabiotic Centre, Biosciences Institute, University College Cork, Cork, Ireland.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Wall
authors.forename: Tatiana M
authors.initials: TM
authors.lastname: Marques
authors.forename: Orla
authors.initials: O
authors.lastname: O'Sullivan
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.forename: Fergus
authors.initials: F
authors.lastname: Shanahan
authors.forename: Eamonn M
authors.initials: EM
authors.lastname: Quigley
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Dinan
authors.forename: Barry
authors.initials: B
authors.lastname: Kiely
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Fitzgerald
authors.forename: Paul D
authors.initials: PD
authors.lastname: Cotter
authors.forename: Fiona
authors.initials: F
authors.lastname: Fouhy
authors.forename: Catherine
authors.initials: C
authors.lastname: Stanton
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001644:Bifidobacterium / Q000145:classification*; D001921:Brain / Q000378:metabolism*; D002849:Chromatography, Gas; D019587:Dietary Supplements; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry*; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology*; D050356:Lipid Metabolism; D054892:Metagenome; D051379:Mice; D008810:Mice, Inbred C57BL; D019936:Probiotics / Q000008:administration & dosage
chemical_list: D005227:Fatty Acids