title: Amber mutants of bacteriophage T4D: their isolation and genetic characterization.
abstract: We have isolated a large number of mutants of bacteriophage T4D that are unable to form plaques on strain B of Escherichia coli, but are able to grow (nearly) normally on some other strains of E. coli, in particular strain CR63. These mutants, designated amber (am), have been characterized by complementation tests, by genetic crosses, and by their response to chemical mutagens. It is concluded that a particular subclass of base substitution mutations may give rise to amber mutants and that such mutants occur in many genes, which are widely distributed over the T4 genome.
authors.affiliation: Laboratory of Biophysics, University of Geneva, CH-1211 Geneva 4, Switzerland
authors.forename: Richard H
authors.initials: RH
authors.lastname: Epstein
authors.affiliation: Laboratory of Biophysics, University of Geneva, CH-1211 Geneva 4, Switzerland
authors.forename: Antoinette
authors.initials: A
authors.lastname: Bolle
authors.affiliation: California Institute of Technology, Pasadena, California 91125, USA
authors.forename: Charles M
authors.initials: CM
authors.lastname: Steinberg
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403-1229, USA. fstahl@uoregon.edu
authors.forename: Franklin W
authors.initials: FW
authors.lastname: Stahl
mesh_terms: D017122:Bacteriophage T4 / Q000235:genetics*; D018389:Codon, Nonsense
chemical_list: D018389:Codon, Nonsense