title: Bostrycin, a novel coupling agent for protein immobilization and prevention of biomaterial-centered infection produced by Nigrospora sp. No. 407.
abstract: Bostrycin, a red antibacterial agent with tetrahydroanthraquinone structure, has been isolated from Nigrospora sp. No. 407. This study investigated the potential antibacterial and multifunctional properties of matrixes through immobilization of bostrycin on their surface for immobilization of protein and prevention of bacterial growth. Bostrycin was immobilized on nonwoven polypropylene (PP) fabric by a technique using glutaraldehyde and polyethyleneimine for the activation of the surface. Glucose oxidase immobilized on bostrycin-treated nonwoven PP fabric showed high activity. The immobilization process improved thermal stability of the enzymes. During repeated assay for 30 cycles, the enzyme activity dropped to only 70% of the initial activity. Both bostrycin-treated nonwoven PP fabric sample and subsequently immobilized glucose oxidase sample on the surface also still exhibited a bacteriostatic effect. This is the first study to show that bostrycin is a promising coupling agent for surface modification on matrix and its potential applications in protein immobilization and biomaterial-centered infection.
authors.affiliation: Institute of Biotechnology, National University of Kaohsiung, Kaohsiung 811, Taiwan.
authors.forename: Wen-Jen
authors.initials: WJ
authors.lastname: Yang
authors.forename: Chih-Sheng
authors.initials: CS
authors.lastname: Yang
authors.forename: Chen-Ji
authors.initials: CJ
authors.lastname: Huang
authors.forename: Ko-Shao
authors.initials: KS
authors.lastname: Chen
authors.forename: Shuen-Fuh
authors.initials: SF
authors.lastname: Lin
mesh_terms: D000880:Anthraquinones / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D001203:Ascomycota / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods; D003014:Clostridium botulinum / Q000187:drug effects; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D011126:Polypropylenes / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D013784:Textiles
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D004800:Enzymes, Immobilized; D011126:Polypropylenes; C034287:bostrycin; D005949:Glucose Oxidase