title: Validation of increased blood storage times with the T-SPOT.TB assay with T-Cell Xtend reagent in individuals with different tuberculosis risk factors.
abstract: We compared the performance of the T-SPOT.TB assay with blood used within 0 to 3.5 h after collection (control) to its performance with blood stored for 0 to 3.5, 5 to 8, 18 to 21, or 31 to 33 h with the addition of T-Cell Xtend (experimental), using samples from 154 participants. The 95.4% concordance between paired specimens indicated that blood can be stored for up to 33 h prior to T-SPOT.TB testing.
authors.affiliation: Department of Internal Medicine, Division of Infectious Diseases, The Ohio State University, Columbus, Ohio, USA. Wang.1055@osu.edu
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: Wang
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Stew
authors.forename: Joshua
authors.initials: J
authors.lastname: Cyktor
authors.forename: Bridget
authors.initials: B
authors.lastname: Carruthers
authors.forename: Joanne
authors.initials: J
authors.lastname: Turner
authors.forename: Blanca I
authors.initials: BI
authors.lastname: Restrepo
mesh_terms: D000328:Adult; D001769:Blood / Q000276:immunology*; D005260:Female; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D059425:Interferon-gamma Release Tests / Q000379:methods*; D008297:Male; D008875:Middle Aged; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D007202:Indicators and Reagents