title: Efficacy of plasma phospholipid removal during sample preparation and subsequent retention under typical UHPLC conditions.
abstract: BACKGROUND
  Several new products have been marketed with the specific capability of removing phospholipids. These products were evaluated alongside more traditional extraction techniques, using UHPLC and precursor-ion scanning (pre m/z 184), which detects glycerophosphocholines (GPCho), lyso-GPCho and sphingomyelins. Using this technique the plasma GPCho profile of human, dog and rat plasma is briefly compared.
  
  
  RESULTS
  Precursor-ion scanning detected more of the phospholipid profile in extracts than a SRM experiment (m/z 184-184). Products designed for the purpose were the most efficient at removing phospholipids and reversed-phase SPE was better than mixed-mode cation exchange. A comparison of different UHPLC columns demonstrated that C(8) or phenyl phases would help manage the elution of GPChos.
  
  
  CONCLUSIONS
  Phospholipid removal plates are useful where no sample concentration is required; however for more challenging LOD it will be necessary to enrich the original sample. In these cases build-up of phospholipids can be avoided with a thoughtful choice of UHPLC column.
authors.affiliation: Quotient Bioresearch, Fordham, Ely, CB7 5WW, UK. david.neville@quotientbioresearch.com
authors.forename: David
authors.initials: D
authors.lastname: Neville
authors.forename: Richard
authors.initials: R
authors.lastname: Houghton
authors.forename: Shane
authors.initials: S
authors.lastname: Garrett
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D059625:Liquid-Liquid Extraction; D010743:Phospholipids / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*; D051381:Rats; D052616:Solid Phase Extraction
chemical_list: D010743:Phospholipids