title: Simultaneous determination of six alkaloids and one monoterpene in rat plasma by liquid chromatography-tandem mass spectrometry and pharmacokinetic study after oral administration of a Chinese medicine Wuji Pill.
abstract: A simple and sensitive method for simultaneous determination of seven active constituents, jatrorrhizine, berberine, coptisine, palmatine, evodiamine, rutacarpine and paeoniflorin, from a Chinese medicine Wuji Pill in rat plasma was developed based on a liquid chromatography and tandem mass spectrometry method. The separation of these seven compounds was carried out on a Shiseido CAPCELL PAK C(18) column using a mobile phase consisting of acetonitrile (containing 0.1% formic acid and water (containing 0.1% formic acid and 10 mmol/L ammonium acetate) and carbamazepine as an internal standard. Electrospray ionization in positive-ion mode and multiple reaction monitoring was used to identify and quantitate active components. All calibration curves gave good linearity (r>0.993) over the concentration range from 0.42-208.0 ng/mL to 4.18-418.0 ng/mL for all components. The precision of the in vivo study was evaluated by intra- and inter-day assays and the percentages of relative standard deviation were all within 15%. The method was successfully applied to pharmacokinetic study of all six alkaloids and one monoterpene in rat plasma after oral administration of the Wuji Pill.
authors.affiliation: Department of Chemistry, School of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yuan
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: An
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Shui-Jun
authors.initials: SJ
authors.lastname: Li
authors.forename: Jing-Ying
authors.initials: JY
authors.lastname: Jia
authors.forename: S W Annie
authors.initials: SW
authors.lastname: Bligh
authors.forename: Xin-Hong
authors.initials: XH
authors.lastname: Wang
authors.forename: Yue-Ming
authors.initials: YM
authors.lastname: Ma
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001600:Berberine Alkaloids / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D016014:Linear Models; D008297:Male; D039821:Monoterpenes / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; D039821:Monoterpenes