title: An experimental model of neonatal normocapnic hypoxia and resuscitation in Landrace/Large White piglets.
abstract: OBJECTIVE
  The aim of this study is to describe and evaluate an experimental model of neonatal normocapnic hypoxia and resuscitation.
  
  
  METHODS
  Ten male Landrace/Large White neonatal piglets were studied. Following anaesthesia and intubation, the animals were mechanically ventilated. Surgical procedures included catheterization of the right internal jugular vein and the carotid artery. After stabilization with 21% O(2), normocapnic hypoxia was induced by decreasing the inspired O(2) to 6-8%. When piglets developed bradycardia (heart rate < 60 beats/min), reoxygenation was initiated by administering 21% O(2). Arterial blood samples were taken during baseline, hypoxia and reoxygenation in order to measure interleukine-6 and interleukine-8.
  
  
  RESULTS
  Nine out of ten animals were successfully resuscitated (one of these required chest compressions and a dose of adrenaline) and one died despite resuscitation efforts. After returning to baseline haemodynamic values, euthanasia was performed using thiopental overdose.
  
  
  CONCLUSIONS
  Haemodynamic fluctuations at baseline, during normocapnic hypoxia and reoxygenation in Landrace/Large White piglets are comparable to that in human neonates, making the breed a favorable model of human neonatal hypoxia investigation.
authors.affiliation: National and Kapodistrian University of Athens, Medical School, Athens, Greece.
authors.forename: Filippia
authors.initials: F
authors.lastname: Aroni
authors.forename: Theodoros
authors.initials: T
authors.lastname: Xanthos
authors.forename: Marianna
authors.initials: M
authors.lastname: Varsami
authors.forename: Ioanna
authors.initials: I
authors.lastname: Argyri
authors.forename: Avgoustina
authors.initials: A
authors.lastname: Alexaki
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Stroumpoulis
authors.forename: Pavlos
authors.initials: P
authors.lastname: Lelovas
authors.forename: Apostolos
authors.initials: A
authors.lastname: Papalois
authors.forename: Gavino
authors.initials: G
authors.lastname: Faa
authors.forename: Vassillios
authors.initials: V
authors.lastname: Fanos
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Iacovidou
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001238:Asphyxia Neonatorum / Q000097:blood / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D001794:Blood Pressure / Q000502:physiology; D004195:Disease Models, Animal; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D000860:Hypoxia / Q000097:blood / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D007231:Infant, Newborn; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D008297:Male; D010100:Oxygen / Q000627:therapeutic use; D012151:Resuscitation / Q000379:methods; D013552:Swine
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D010100:Oxygen