title: ROPGEF1 and ROPGEF4 are functional regulators of ROP11 GTPase in ABA-mediated stomatal closure in Arabidopsis.
abstract: ROPs constitute a family of plant-specific, RHO-like small GTPases that serve as molecular switches in a wide range of signaling pathways. The activities of ROPs are regulated by guanine nucleotide exchange factors (GEFs). ROP11, a member of the ROP GTPase family in Arabidopsis, is a negative regulator of multiple ABA responses. In this study, we show that ROPGEF1 and ROPGEF4 interact with ROP11 on plasma membranes in guard cells. Furthermore, our analyses of ROPGEF1/4 knockout mutants and overexpressing lines suggested that ROPGEF1 and ROPGEF4 are specific regulators of ROP11 function in ABA-mediated stomatal closure.
authors.affiliation: MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Zixing
authors.initials: Z
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000033:anatomy & histology / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000378:metabolism*; D021381:Protein Transport; D015398:Signal Transduction; D013312:Stress, Physiological; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D020662:Guanine Nucleotide Exchange Factors; C572956:ROPGEF4 protein, Arabidopsis; C553927:RopGEF1 protein, Arabidopsis; D000040:Abscisic Acid; C572957:ROP11 protein, Arabidopsis; D020741:rho GTP-Binding Proteins