title: Microarray-based detection of specific IgE against recombinant ω-5-gliadin in suspected wheat-dependent exercise-induced anaphylaxis.
abstract: Wheat-dependent exercise-induced anaphylaxis (WDEIA) is a rare IgE-mediated food allergy. Component-resolved measurement of specific IgE (sIgE) against ω-5-gliadin by fluorescence enzyme immunoassay (FEIA) has been postulated as a good predictive decision criterion in the diagnosis of WDEIA. More recently, microarray technology has been introduced into component-resolved diagnostics. The aim of this study was to evaluate the performance of an allergen microarray in the detection of sIgE against ω-5-gliadin in 10 patients with suspected WDEIA and high levels of sIgE against ω-5-gliadin (mean: 9.31±7.53 kU/L, range: 4.24-25.8) as measured by FEIA. Using an old version of the microarray assay (ImmunoCAP ISAC™, Phadia), sIgE against ω-5-gliadin was detected in only 3 of the first 6 patients. The same samples and those of another 4 patients were then analysed with an improved version of the microarray system, yielding elevated levels of sIgE against ω-5-gliadin in all patients. In conclusion, the old version of the microarray was not reliable for the detection of sIgE against ω-5-gliadin in the examined individuals with suspected WDEIA. In contrast, the improved version of the microarray seems to be as adequate as FEIA in the detection of sIgE against ω-5-gliadin. However, further large-scale studies are warranted to confirm these results.
authors.affiliation: Department of Dermatology and Allergology, University Hospital Aachen, Germany. rbrans@uos.de
authors.forename: Richard
authors.initials: R
authors.lastname: Brans
authors.forename: Ilka
authors.initials: I
authors.lastname: Sauer
authors.forename: Katharina
authors.initials: K
authors.lastname: Czaja
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Pfützner
authors.forename: Hans F
authors.initials: HF
authors.lastname: Merk
mesh_terms: D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology*; D000707:Anaphylaxis / Q000276:immunology*; D052179:Antigens, Plant; D015444:Exercise / Q000502:physiology; D005260:Female; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D011994:Recombinant Proteins / Q000276:immunology; D021182:Wheat Hypersensitivity / Q000276:immunology*
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D011994:Recombinant Proteins; C475180:omega-5 gliadin, wheat; D007073:Immunoglobulin E; D005903:Gliadin