title: Apoptin induces apoptosis in human bladder cancer EJ and BIU-87 cells.
abstract: OBJECTIVE
  To investigate whether apoptin is a apoptosis-inducing protein with a potential for bladder cancer therapy.
  
  
  METHODS
  We constructed a PCDNA3/Apoptin eukaryotic expression vector, and transfected this vector into bladder cancer cell lines BIU-87 and EJ, then observed the results by RT-PCR, transmission electron microscopy, MTT assay and the flow cytometry (TUNEL method).
  
  
  RESULTS
  PCDNA3/Apoptin successfully induced a high level apoptosis in both bladder cancer cell lines, compared with the controls (p<0.05).
  
  
  CONCLUSIONS
  Apoptin can induce high level apoptosis in human bladder cancer EJ and BIU-87 cells, which suggests a potential for human bladder cancer therapy.
authors.affiliation: Department of Urology, Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhan
authors.forename: Jian-Song
authors.initials: JS
authors.lastname: Wang
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Wang
authors.forename: Yi-Gang
authors.initials: YG
authors.lastname: Zuo
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Wang
authors.forename: Ming-Xia
authors.initials: MX
authors.lastname: Ding
mesh_terms: D017209:Apoptosis; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D017727:Chicken anemia virus; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: D036022:Capsid Proteins; D012333:RNA, Messenger; C091717:VP3 protein, Chicken anemia virus