title: Laccase- and chloroperoxidase-nanotube paint composites with bactericidal and sporicidal activity.
abstract: Laccase and chloroperoxidase (CPO) were separately immobilized onto multi-walled carbon nanotubes (MWNTs) and subsequently mixed with a commercial eco-friendly paint to generate biocatalytic coatings. The laccase-nanotube based paints showed >99% bactericidal activity against Escherichia coli and Staphylococcus aureus (both challenged with 10⁶ CFU/mL) within 30 min and >98% sporicidal activity against Bacillus cereus and Bacillus anthracis-ΔSterne (initially challenged with 10⁴ CFU/mL) within 120 min. The CPO-nanotube based paints also showed >99% antimicrobial activity within 30 min against E. coli and S. aureus (both challenged with 10⁶ CFU/mL). These enzyme-nanotube based formulations provide an eco-friendly route to generate biocidal compounds, which can prevent the growth of a broad spectrum of bacterial pathogens, including spores. These enzyme-containing paints may be envisioned to be applied as self-decontaminating coatings onto a wide range of surfaces, such as hospital infrastructure, medical devices and equipment, food processing and packaging, etc.; in all cases effective killing of a variety of infectious organisms is critical.
authors.affiliation: Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Navdeep
authors.initials: N
authors.lastname: Grover
authors.forename: Indrakant V
authors.initials: IV
authors.lastname: Borkar
authors.forename: Cerasela Zoica
authors.initials: CZ
authors.lastname: Dinu
authors.forename: Ravi S
authors.initials: RS
authors.lastname: Kane
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Dordick
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001408:Bacillus anthracis / Q000187:drug effects; D001409:Bacillus cereus / Q000187:drug effects; D001709:Biotechnology / Q000379:methods; D002711:Chloride Peroxidase / Q000737:chemistry* / Q000378:metabolism; D015169:Colony Count, Microbial; D003470:Culture Media; D004800:Enzymes, Immobilized; D004926:Escherichia coli / Q000187:drug effects; D006997:Hypochlorous Acid / Q000378:metabolism / Q000494:pharmacology; D007455:Iodine / Q000378:metabolism / Q000494:pharmacology; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010150:Paint; D013171:Spores, Bacterial / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D006997:Hypochlorous Acid; D007455:Iodine; D042845:Laccase; D002711:Chloride Peroxidase