title: Ocular pathogenesis and immune reaction after intravitreal dispase injection in mice.
abstract: OBJECTIVE
  The purpose of the current study was to examine the ocular pathogenesis and immune reaction in mice after intravitreal dispase injection.
  
  
  METHODS
  Three microliters of dispase at a concentration of 0.2 U/μl were injected into the vitreal cavities of 4-6-week-old mice. Hematoxylin and eosin staining, immunofluorescence analysis, and electroretinograms of the eyes were then performed to assess ocular changes, and enzyme-linked immunospot assays and intracellular staining of single-cell suspensions of the spleens were used to detect immune changes during an 8 week observation period.
  
  
  RESULTS
  Neutrophils were the main inflammatory infiltrating cells appearing at the anterior chamber. No cluster of differentiation (CD)3+ labeled T cells, F4/80+ labeled macrophages, or CD56+ labeled natural killer cells were found in the vitreal cavities or retinas in dispase-injected mice within 5 days after injection. Proliferative vitreoretinopathy (PVR)-like signs first appeared at 2 weeks, gradually increased thereafter, and reached peak values at 8 weeks. There was a statistically significant difference in b-wave amplitudes between the PVR and saline-control eyes. Enzyme-linked immunospot assays and intracellular staining showed that specific CD4+ and CD8+ labeled T cells were not involved in dispase-injected mice.
  
  
  CONCLUSIONS
  Our data show that neutrophils in the anterior chamber and PVR-like signs in the retinas were found, and that specific immune reactions were not involved after intravitreal dispase injection in mice.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Tan
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Qianying
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004596:Electroretinography; D010450:Endopeptidases; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D058449:Intravitreal Injections; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000276:immunology / Q000473:pathology; D012160:Retina / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000276:immunology / Q000473:pathology; D018630:Vitreoretinopathy, Proliferative / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*
chemical_list: D010450:Endopeptidases; C023139:dispase; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin