title: Spectral-domain optical coherence tomography as a noninvasive method to assess damaged and regenerating adult zebrafish retinas.
abstract: OBJECTIVE
  These experiments assessed the ability of spectral-domain optical coherence tomography (SD-OCT) to accurately represent the structural organization of the adult zebrafish retina and reveal the dynamic morphologic changes during either light-induced damage and regeneration of photoreceptors or ouabain-induced inner retinal damage.
  
  
  METHODS
  Retinas of control dark-adapted adult albino zebrafish were compared with retinas subjected to 24 hours of constant intense light and recovered for up to 8 weeks or ouabain-damaged retinas that recovered for up to 3 weeks. Images were captured and the measurements of retinal morphology were made by SD-OCT, and then compared with those obtained by histology of the same eyes.
  
  
  RESULTS
  Measurements between SD-OCT and histology were very similar for the undamaged, damaged, and regenerating retinas. Axial measurements of SD-OCT also revealed vitreal morphology that was not readily visualized by histology.
  
  
  CONCLUSIONS
  SD-OCT accurately represented retinal lamination and photoreceptor loss and recovery during light-induced damage and subsequent regeneration. SD-OCT was less accurate at detecting the inner nuclear layer in ouabain-damaged retinas, but accurately detected the undamaged outer nuclear layer. Thus, SD-OCT provides a noninvasive and quantitative method to assess the morphology and the extent of damage and repair in the zebrafish retina.
authors.affiliation: Department of Biological Sciences and the Center for Zebrafish Research, University of Notre Dame, Notre Dame, IN 46556, USA.
authors.forename: Travis J
authors.initials: TJ
authors.lastname: Bailey
authors.forename: Darin H
authors.initials: DH
authors.lastname: Davis
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Vance
authors.forename: David R
authors.initials: DR
authors.lastname: Hyde
mesh_terms: D000818:Animals; D003623:Dark Adaptation; D004195:Disease Models, Animal; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology; D020127:Recovery of Function; D012038:Regeneration; D012160:Retina / Q000502:physiology*; D012164:Retinal Diseases / Q000473:pathology* / Q000503:physiopathology; D041623:Tomography, Optical Coherence / Q000379:methods*; D015027:Zebrafish