title: Expression and clinical significance of von Hippel-Lindau downstream genes: Jade-1 and β-catenin related to renal cell carcinoma.
abstract: OBJECTIVE
  To investigate the expression of Jade-1 and β-catenin and their effect in the development of renal cell carcinoma (RCC).
  
  
  METHODS
  The expression of Jade-1 and β-catenin in 11 normal kidney tissue specimens (normal group) and 60 RCC specimens (RCC group) was determined using real-time polymerase chain reaction and immunohistochemistry. Also, their effect on early relapses of RCC was analyzed after 5 years of follow-up.
  
  
  RESULTS
  The expression of Jade-1 protein in the RCC group was significantly lower than that in the normal group (0.1655 vs 0.7438, P < .05), and the expression of β-catenin protein was significantly greater than that in the normal group (0.2756 vs 0.0855, P < .05). The expression of Jade-1 mRNA in the RCC group was 0.202 times that in the normal group, which were lower (P < .05). The expression of β-catenin mRNA was 1.014 times that in the normal group (P > .05). The expression of Jade-1 protein and β-catenin protein in poorly differentiated RCC specimens was significantly lower and higher than the expression in the well-differentiated RCC specimen (P < .05), respectively. Patients with negative Jade-1 expression and positive β-catenin expression were found to have shorter survival on univariate analysis (P < .05).
  
  
  CONCLUSIONS
  RCC with a low expression of Jade-1 and high expression of β-catenin is associated with a poor outcome and decreased survival.
authors.affiliation: Department of Urology, Lishui Central Hospital, Wenzhou Medical College, Zhejiang, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lian
authors.forename: Xiuzhi
authors.initials: X
authors.lastname: Duan
authors.forename: Xiuling
authors.initials: X
authors.lastname: Wu
authors.forename: Chengdi
authors.initials: C
authors.lastname: Li
authors.forename: Shengye
authors.initials: S
authors.lastname: Chen
authors.forename: Siqi
authors.initials: S
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Cai
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Weng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000401:mortality; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D015996:Survival Rate; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D051176:beta Catenin / Q000235:genetics*
chemical_list: D018398:Homeodomain Proteins; C469360:JADE1 protein, human; D025521:Tumor Suppressor Proteins; D051176:beta Catenin