title: [Complete genome sequence characteristics of human enterovirus 71 strain isolated in Yunnan, China].
abstract: The complete nucleotide sequence of two human enterovirus 71 strains (KMM09 and KM186-09) isolated in Yunnan,China, were determined by RT-PCR and sequencing. As with other human enteroviruses, the genomes were 7 409 nucleotides (nts) in length and encoded 2 193 aa. Phylogenetic analysis based on VP1 regions revealed that the two isolates belonged to subgenotype C4a. In structural genomic regions, subgenotype C4 was most homologous to other strains of C genotype when compared to other genotypes. In non-structural genomic regions, subgenotype C4 was more homologous to CA16/G10 and other strains of B genotype than to other strains of C genotype. RDP3 and Blast analysis displayed evidence of recombination in non-structural genomic regions between subgenotype B3 and C4, C4 and CA16/G10. The full-length genome of the human enterovirus 71 strains provided an overview of the diversity of genetic characteristics of a circulatinghuman enterovirus 71.
authors.affiliation: Institute of Medical Biology, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, China. huali10101@163.com
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Cong-Wen
authors.initials: CW
authors.lastname: Shao
authors.forename: Yue
authors.initials: Y
authors.lastname: Pan
authors.forename: Hua-Xin
authors.initials: HX
authors.lastname: Ke
authors.forename: Shao-Hui
authors.initials: SH
authors.lastname: Ma
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D002522:Chlorocebus aethiops; D029821:Enterovirus A, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D014709:Vero Cells