title: Transgenic chimera quail production by microinjecting lentiviral vector into the blood vessel of the early embryo.
abstract: In the past, several strategies have been used to generate transgenic birds. The most successful method has proven to be injection of lentiviral vector into the subgerminal cavity of the newly laid egg. In this study, we directly injected lentiviral vector into the blood vessel of HH13-15 quail embryos to produce transgenic chimeras. In the manipulated, hatched birds, the green fluorescent protein (GFP) gene driven by a cytomegalovirus (CMV) promoter was extensively expressed. All tissues analyzed were GFP-positive, and gonad cells from some of the manipulated embryos expressed GFP. The semen genome of 21.4% of mature male birds was determined to be GFP-positive by PCR, indicating these male birds were transgenic chimeras.
authors.affiliation: State Key Laboratories for Agrobiotechnology, China Agricultural University Beijing Education Examinations Authority, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.forename: Zifu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Guojin
authors.initials: G
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.forename: Wenxin
authors.initials: W
authors.lastname: Zhang
authors.forename: Junshuang
authors.initials: J
authors.lastname: Gao
authors.forename: Wenjing
authors.initials: W
authors.lastname: Jin
authors.forename: Zandong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D015153:Blotting, Western; D002678:Chimera / Q000235:genetics*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007150:Immunohistochemistry; D016086:Lentivirus; D008297:Male; D008845:Microinjections; D016133:Polymerase Chain Reaction; D011784:Quail / Q000196:embryology / Q000235:genetics*
chemical_list: D049452:Green Fluorescent Proteins