title: Radical gas-based DNA decontamination for ultra-sensitive molecular experiments.
abstract: In this study, we tested a radical gas-based decontamination technique to prevent possible DNA contamination by the air and/or equipment used in molecular experiments. We prepared 10(4) molecules of model DNA contaminant and placed the dried DNA into test tubes, which were then exposed to radical gas. Quantitative PCR analysis showed that, even after a short exposure time of 30 minutes, 99.54% of the model DNA contaminant was effectively decomposed to undetectable levels. Our results demonstrate that the radical gas-based treatment is a useful method for eliminating potential DNA contaminant in ultra-sensitive molecular experiments.
authors.affiliation: Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology, Monobe B200, Nankoku, Kochi 783–8502, Japan. morono@jamsetc.go.jp
authors.forename: Yuki
authors.initials: Y
authors.lastname: Morono
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Fumio
authors.initials: F
authors.lastname: Inagaki
mesh_terms: D004247:DNA / Q000737:chemistry*; D003666:Decontamination / Q000379:methods*; D005740:Gases; D000432:Methanol / Q000737:chemistry; D016133:Polymerase Chain Reaction; D043902:Pyrococcus horikoshii / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis
chemical_list: D005740:Gases; D012336:RNA, Ribosomal, 16S; D004247:DNA; D000432:Methanol