title: A PLETHORA-auxin transcription module controls cell division plane rotation through MAP65 and CLASP.
abstract: Despite their pivotal role in plant development, control mechanisms for oriented cell divisions have remained elusive. Here, we describe how a precisely regulated cell division orientation switch in an Arabidopsis stem cell is controlled by upstream patterning factors. We show that the stem cell regulatory PLETHORA transcription factors induce division plane reorientation by local activation of auxin signaling, culminating in enhanced expression of the microtubule-associated MAP65 proteins. MAP65 upregulation is sufficient to reorient the cortical microtubular array through a CLASP microtubule-cell cortex interaction mediator-dependent mechanism. CLASP differentially localizes to cell faces in a microtubule- and MAP65-dependent manner. Computational simulations clarify how precise 90° switches in cell division planes can follow self-organizing properties of the microtubule array in combination with biases in CLASP localization. Our work demonstrates how transcription factor-mediated processes regulate the cellular machinery to control orientation of formative cell divisions in plants.
authors.affiliation: Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. p.b.dhonukshe@uu.nl
authors.forename: Pankaj
authors.initials: P
authors.lastname: Dhonukshe
authors.forename: Daan A
authors.initials: DA
authors.lastname: Weits
authors.forename: Alfredo
authors.initials: A
authors.lastname: Cruz-Ramirez
authors.forename: Eva E
authors.initials: EE
authors.lastname: Deinum
authors.forename: Simon H
authors.initials: SH
authors.lastname: Tindemans
authors.forename: Klementina
authors.initials: K
authors.lastname: Kakar
authors.forename: Kalika
authors.initials: K
authors.lastname: Prasad
authors.forename: Ari Pekka
authors.initials: AP
authors.lastname: Mähönen
authors.forename: Chris
authors.initials: C
authors.lastname: Ambrose
authors.forename: Michiko
authors.initials: M
authors.lastname: Sasabe
authors.forename: Guy
authors.initials: G
authors.lastname: Wachsmann
authors.forename: Marijn
authors.initials: M
authors.lastname: Luijten
authors.forename: Tom
authors.initials: T
authors.lastname: Bennett
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Machida
authors.forename: Renze
authors.initials: R
authors.lastname: Heidstra
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Wasteneys
authors.forename: Bela M
authors.initials: BM
authors.lastname: Mulder
authors.forename: Ben
authors.initials: B
authors.lastname: Scheres
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002455:Cell Division; D007210:Indoleacetic Acids / Q000378:metabolism; D018519:Meristem / Q000166:cytology / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D059828:Plant Cells / Q000378:metabolism*; D019441:Plant Epidermis / Q000166:cytology / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C527272:CLASP protein, Arabidopsis; D007210:Indoleacetic Acids; C492901:MAP65-1 protein, Arabidopsis; C572577:MAP65-2 protein, Arabidopsis; D008869:Microtubule-Associated Proteins; D014157:Transcription Factors