title: Study of DNA-emodin interaction by FTIR and UV-vis spectroscopy.
abstract: Emodin, a plant- and fungus-derived anthraquinone, exerts genotoxic and antioxidative effects and shows promise in antitumor and antibacterial therapies. The aim of this study was to examine the molecular interactions of emodin with DNA in aqueous solution at physiological pH using spectroscopic methods. Fourier Transform Infrared (FTIR) Spectroscopy and UV absorption spectra were used to determine the structural features, the binding mode and the association constants. Our UV-spectroscopic results indicate that emodin interacts with DNA by intercalation and by external binding. FTIR results suggest that emodin interaction occurs preferably via adenine and thymine base pairs and also weakly with the phosphate backbone of the DNA double helix. The binding constant for emodin-DNA complex formation is estimated to be K=5.59×10(3)M(-1). No significant changes of DNA conformation were observed upon emodin-DNA complexation.
authors.affiliation: Laboratório de Biologia de Fungos, Centro de Biotecnologia e Genética, Universidade Estadual de Santa Cruz, Ilhéus, Bahia, Brazil.
authors.forename: Samuel T
authors.initials: ST
authors.lastname: Saito
authors.forename: Givaldo
authors.initials: G
authors.lastname: Silva
authors.forename: Cristina
authors.initials: C
authors.lastname: Pungartnik
authors.forename: Martin
authors.initials: M
authors.lastname: Brendel
mesh_terms: D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D004642:Emodin / Q000737:chemistry*; D008968:Molecular Conformation; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004247:DNA; D004642:Emodin