title: Chimeric hexon HVRs protein reflects partial function of adenovirus.
abstract: Adenovirus is widely used in gene therapy and vaccination as a viral vector, and its hypervariable regions (HVRs) on hexon are the main antigen recognition sites of adenovirus. The modification of this area by genetic engineering will change the antigenic specificity of the virus. In addition, recent studies have demonstrated the importance of coagulation factor X (FX) in adenovirus serotype 5-mediated liver transduction in vivo. The binding site of adenovirus to FX is the HVRs on hexon. By constructing five proteins containing chimeric HVRs from different adenovirus serotypes, we focused on the antigenic specificity and the affinity for FX of these proteins compared with the corresponding viruses. Our data showed that HVR5 and HVR7 had only a part of hexon activity to neutralizing antibodies (NAbs) compared with the complete activity of HVR1-7. Results also demonstrated a differential high-affinity interaction of the HVRs proteins with FX and indicated that HVRs protein had a similar binding ability with corresponding adenovirus serotype. These results highlighted some properties of chimeric HVRs proteins and revealed the influence on the structure and function of hexon proteins and adenovirus resulting from the HVRs.
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, College of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.forename: Chu
authors.initials: C
authors.lastname: Wang
authors.forename: Jianing
authors.initials: J
authors.lastname: Dong
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Haihong
authors.initials: H
authors.lastname: Zhang
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Wu
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.forename: Xianghui
authors.initials: X
authors.lastname: Yu
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D057134:Antibodies, Neutralizing / Q000276:immunology; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000939:Epitopes; D005170:Factor X / Q000276:immunology*; D015316:Genetic Therapy; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D036022:Capsid Proteins; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D014765:Viral Vaccines; C018315:hexon capsid protein, Adenovirus; D005170:Factor X