title: Essential functions of C terminus of Drosophila Topoisomerase IIIα in double holliday junction dissolution.
abstract: Topoisomerase IIIα (Top3α) is an essential component of the double Holliday junction (dHJ) dissolvasome complex in metazoans, along with Blm and Rmi1/2. This important anti-recombinogenic function cannot be performed by Top3β, the other type IA topoisomerase present in metazoans. The two share a catalytic core but diverge in their tail regions. To understand this difference in function, we investigated the role of the unique C terminus of Top3α. The Drosophila C terminus contains an insert region not conserved among metazoans. This insert contributes an independent interaction with Blm, which may account for the absence of Rmi1 in Drosophila. Mutant Top3α lacking this insert maintains the ability to perform dHJ dissolution but only partially rescues a top3α null fly line, indicating an in vivo role for the insert. Truncation of the C terminus has a minimal effect on the type IA relaxation activity of Top3α; however, dHJ dissolution is greatly reduced. The Top3α C terminus was found to strongly interact with both Blm and DNA, which are critical to the dissolution reaction; these interactions are greatly reduced in the truncated enzyme. The truncation mutant also cannot rescue the viability of top3α null flies, indicating an essential in vivo role. Our data therefore suggest that the Top3α C terminus has an important role in dHJ dissolution (by providing an interaction interface for Blm and DNA) and an essential function in vivo.
authors.affiliation: Department of Biochemistry, Duke University, Durham, North Carolina 27710, USA.
authors.forename: Stefanie Hartman
authors.initials: SH
authors.lastname: Chen
authors.forename: Chung-Hsuan
authors.initials: CH
authors.lastname: Wu
authors.forename: Jody L
authors.initials: JL
authors.lastname: Plank
authors.forename: Tao-shih
authors.initials: TS
authors.lastname: Hsieh
mesh_terms: D000818:Animals; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000235:genetics / Q000378:metabolism*; D045566:DNA, Cruciform / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D009154:Mutation; D017434:Protein Structure, Tertiary
chemical_list: D045566:DNA, Cruciform; D029721:Drosophila Proteins; D004265:DNA Helicases; C470639:blm protein, Drosophila; D004264:DNA Topoisomerases, Type I