title: [HPLC fingerprint analysis of acetoacetate extraction of Polygonum orientale].
abstract: OBJECTIVE
  To establish the HPLC fingerprint analysis method for the quality control of acetoacetate extraction of Polygonum orientale.
  
  
  METHODS
  Agilent C18 (4.6 mm x 250 mm, 5 microm); DAD detector; Mobile phase: methyl alcohol-water gradient elution at a flow rate of 1.0 mL/min, and at a column temperature of 30 degrees C.
  
  
  RESULTS
  26 peaks were selected as the common fingerprint peaks. The relative standard deviations for relative retention values were less than 3% in the precision and repeated test.
  
  
  CONCLUSIONS
  The repetition, stability and precision of the method is good and it can be used to control the quality of Polygonum orientale.
authors.affiliation: School of Chemistry and Engineering, Wuhan University of Technology, Wuhan 430070, China. hujiaxing2008@163.com
authors.forename: Jia-Xing
authors.initials: JX
authors.lastname: Hu
authors.forename: Yi-Ceng
authors.initials: YC
authors.lastname: Lou
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.forename: Du-Qian
authors.initials: DQ
authors.lastname: Zhang
mesh_terms: D000090:Acetoacetates / Q000737:chemistry; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D028502:Polygonum / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D000090:Acetoacetates; D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; C005217:methyl acetoacetate