title: 5-flourouracil loaded N,O-carboxymethyl chitosan nanoparticles as an anticancer nanomedicine for breast cancer.
abstract: Chitosan and its carboxymethyl derivatives are smart biopolymers that are non-toxic, biocompatible, biodegradable and hence found applications in biomedical field. In the current work, we have developed 5-fluorouracil (5-FU) loaded N,Ocarboxymethyl chitosan (N,O-CMC) nanoparticles (mean diameter: 80 +/- 20 nm, zeta potential: +52.47 +/- 2 mV) for cancer drug delivery. Drug entrapment efficiency (65%) and in vitro drug release studies were carried out spectrophotometricaly. Cellular internalization of the drug loaded nanoparticles was confirmed by fluorescent microscopy and flow cytometric analysis. Results of anticancer activity via MTT, apoptosis and caspase 3 assays showed the toxicity of the drug loaded nanoparticles towards breast cancer cells. As a whole these results indicates the potential of 5-FU loaded N,O-CMC nanoparticles in breast cancer chemotherapy in which the side effects of conventional chemo treatment could be reduced. Furthermore, the results of in vitro hemolytic assay and coagulation assay substantiate the blood compatibility of the system as well.
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Cochin 682041, India.
authors.forename: A
authors.initials: A
authors.lastname: Anitha
authors.forename: K P
authors.initials: KP
authors.lastname: Chennazhi
authors.forename: S V
authors.initials: SV
authors.lastname: Nair
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects; D001780:Blood Coagulation Tests; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry; D005472:Fluorouracil / Q000008:administration & dosage* / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size
chemical_list: D000964:Antimetabolites, Antineoplastic; D004337:Drug Carriers; C077199:O,N-carboxymethylchitosan; D048271:Chitosan; D053148:Caspase 3; D005472:Fluorouracil