title: Silver-loaded biomimetic hydroxyapatite grafted poly(epsilon-caprolactone) composite nanofibers: a cytotoxicity study.
abstract: We report on poly(epsilon-caprolactone) (PCL) containing biomimetic hydroxyapatite (HA) and hydroxyapatite-silver (HA-Ag) composite nanofibers prepared via an electrospinning process for the biomedical applications. The morphology, structure and thermal properties of the PCL, PCL/HA and PCL/HA-Ag composite nanofibers were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectroscopy, X-ray diffraction (XRD) and thermogravimetry (TGA). SEM images revealed that the nanofibers were well-oriented and incorporated the HA-Ag nanoparticles well. The adhesion, viability and proliferation properties of composite nanofibers and differentiation of osteoblast were carried out to study the in vitro cell compatibility of the PCL/HA/HA-Ag composite nanofibers. Our results showed that pristine PCL and PCL/HA composite nanofibers scaffold can be utilized for the bone regeneration applications, however, PCL/HA-Ag composite nanofibers results in mild cytotoxic effect to human cells.
authors.affiliation: Department of Organic Materials and Fiber Engineering, College of Engineering, Chonbuk National University, Jeonju, 561 756, South Korea.
authors.forename: R
authors.initials: R
authors.lastname: Nirmala
authors.forename: Hyung-Sub
authors.initials: HS
authors.lastname: Kang
authors.forename: Hye-Min
authors.initials: HM
authors.lastname: Park
authors.forename: R
authors.initials: R
authors.lastname: Navamathavan
authors.forename: In Soo
authors.initials: IS
authors.lastname: Jeong
authors.forename: Hak Yong
authors.initials: HY
authors.lastname: Kim
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D002417:Cattle; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry / Q000633:toxicity*; D055119:Elastic Modulus; D006801:Humans; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry / Q000633:toxicity*; D016062:Porosity; D012834:Silver / Q000737:chemistry / Q000633:toxicity*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D012834:Silver; D017886:Durapatite