title: RT-PCR test for detecting porcine sapovirus in weanling piglets in Hunan Province, China.
abstract: The prevalence of porcine sapovirus infection in weanling pigs was investigated in Hunan Province, China, between August 2006 and October 2007. A total of 153 diarrheic fecal samples from ten intensive pig farms from ten representative administrative regions in Hunan province were examined for porcine sapoviruses using RT-PCR. Twenty-two of 153 (14.37 %) samples were found to contain porcine sapoviruses. Phylogenetic analysis showed that all the porcine sapovirus isolates in Hunan Province belonged to the porcine sapovirus genogroup III. The results of the present investigation have implications for the control of porcine sapovirus infection in pigs in Hunan Province, China.
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, 410128, People's Republic of China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Liu
authors.forename: Run-Cheng
authors.initials: RC
authors.lastname: Li
authors.forename: Ze-Bin
authors.initials: ZB
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Chao-Ting
authors.initials: CT
authors.lastname: Xiao
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Man-Xiang
authors.initials: MX
authors.lastname: Li
authors.forename: Yun-Qiu
authors.initials: YQ
authors.lastname: Yan
authors.forename: Xing-Long
authors.initials: XL
authors.lastname: Yu
mesh_terms: D000818:Animals; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D002681:China / Q000453:epidemiology; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D029341:Sapovirus / Q000235:genetics* / Q000302:isolation & purification; D017423:Sequence Analysis, RNA / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology
chemical_list: D012367:RNA, Viral