title: Phytochemical studies and antioxidant activity of two South African medicinal plants traditionally used for the management of opportunistic fungal infections in HIV/AIDS patients.
abstract: BACKGROUND
  It has been observed that perturbations in the antioxidant defense systems, and consequently redox imbalance, are present in many tissues of HIV-infected patients. Hence, the exogenous supply of antioxidants, as natural compounds that scavenge free radicals, might represent an important additional strategy for the treatment of HIV infection. The aim of this study was therefore to analyse the phytochemical constituents and antioxidant potential of Gasteria bicolor Haw and Pittosporum viridiflorum Sims., two South African plants traditionally used for the management of opportunistic fungal infections (OFIs) in AIDS patients.
  
  
  METHODS
  The in vitro antioxidant properties of the two plants were screened through DPPH (1,1-diphenyl-2-picrylhydrazyl), NO (nitric oxide), H2O2 (hydrogen peroxide) radical scavenging effects and reducing power assays. Phytochemical studies were done by spectrophotometric techniques.
  
  
  RESULTS
  There were no significant differences in the flavonoid and proanthocyanidins contents between the leaves and bark extracts of Gasteria bicolor and Pittosporum viridiflorum respectively, while the total phenolic content of the bark extract of P. viridiflorum was significantly higher than that of G. bicolor leaf. The acetone extracts of both plants indicated strong antioxidant activities.
  
  
  CONCLUSIONS
  The results from this study indicate that the leaves and stem extracts of Gasteria bicolor and Pittosporum viridiflorum respectively possess antioxidant properties and could serve as free radical inhibitors, acting possibly as primary antioxidants. Since reactive oxygen species are thought to be associated with the pathogenesis of AIDS, and HIV-infected individuals often have impaired antioxidant defenses, the inhibitory effect of the extracts on free radicals may partially justify the traditional use of these plants in the management of OFIs in HIV patients in South Africa.
authors.affiliation: Department of Botany, School of Biological and Environmental Sciences, Faculty of Science and Agriculture, University of Fort Hare, Alice 5700, South Africa.
authors.forename: Wilfred Mbeng
authors.initials: WM
authors.lastname: Otang
authors.forename: Donald Scott
authors.initials: DS
authors.lastname: Grierson
authors.forename: Roland Ndip
authors.initials: RN
authors.lastname: Ndip
mesh_terms: D000935:Antifungal Agents / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D015658:HIV Infections / Q000150:complications / Q000188:drug therapy / Q000382:microbiology; D006801:Humans; D019668:Liliaceae / Q000737:chemistry*; D009894:Opportunistic Infections / Q000188:drug therapy / Q000382:microbiology; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D018515:Plant Leaves; D010946:Plants, Medicinal / Q000737:chemistry; D044945:Proanthocyanidins / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D019685:Rosales / Q000737:chemistry*; D013019:South Africa
chemical_list: D000935:Antifungal Agents; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D044945:Proanthocyanidins