title: Biocompatibility and biomineralization assessment of a new root canal sealer and root-end filling material.
abstract: OBJECTIVE
  This study investigated the short-term subcutaneous tissue reaction and biomineralization ability of two epoxy-based root canal sealers containing calcium hydroxide (MBP and MBPc) and ProRoot MTA.
  
  
  METHODS
  Polyethylene tubes containing the materials were implanted into the dorsal connective tissue of Wistar rats (n = 52) for 7 or 30 days; empty implanted tubes served as controls. Specimens were stained with hematoxylin-eosin and von Kossa stain or left unstained for observation under polarized light. Qualitative and quantitative evaluations of all tissue reactions were performed. One-way anova and the Kruskal-Wallis test were used for statistical analysis (P < 0.05).
  
  
  RESULTS
  No significant differences were observed among the groups. All three materials induced mild-to-moderate tissue reactions at 7 days, which decreased over time. Dystrophic mineralization and birefringent structures were observed only in the ProRoot MTA (®) group.
  
  
  CONCLUSIONS
  Both MBP and MBPc appear to be biocompatible but do not stimulate biomineralization.
authors.affiliation: Faculdade de Odontologia de Araçatuba, UNESP - Univ Estadual Paulista, Department of Endodontics, Araçatuba, São Paulo, Brazil. lucianocintra@foa.unesp.br
authors.forename: Luciano Tavares Angelo
authors.initials: LT
authors.lastname: Cintra
authors.forename: Thiago Alexandre Alves
authors.initials: TA
authors.lastname: Ribeiro
authors.forename: João Eduardo
authors.initials: JE
authors.lastname: Gomes-Filho
authors.forename: Pedro Felício Estrada
authors.initials: PF
authors.lastname: Bernabé
authors.forename: Simone
authors.initials: S
authors.lastname: Watanabe
authors.forename: Aguinaldo Cândido da Silva
authors.initials: AC
authors.lastname: Facundo
authors.forename: Renata Oliveira
authors.initials: RO
authors.lastname: Samuel
authors.forename: Eloi
authors.initials: E
authors.lastname: Dezan-Junior
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001843:Bone Cements; D017610:Calcium Compounds / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D003239:Connective Tissue Cells / Q000187:drug effects*; D004338:Drug Combinations; D007249:Inflammation / Q000139:chemically induced*; D008297:Male; D010087:Oxides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000737:chemistry* / Q000494:pharmacology; D012389:Root Canal Obturation / Q000379:methods; D017640:Silicates / Q000494:pharmacology*; D014089:Tooth Remineralization / Q000379:methods*
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D001843:Bone Cements; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D002126:Calcium Hydroxide