title: Estimation of lipoprotein profile in patients with type II diabetes and its relevance to remnant lipoprotein cholesterol levels.
abstract: BACKGROUND
  Remnant lipoprotein (RLP), associated with atherosclerosis progression, is often elevated in diabetes mellitus. The RLP level is estimated by immune-separation method and agarose-gel electrophoresis (AGE).
  
  
  METHODS
  The patients were grouped into three groups according to tertile of RLP-cholesterol (RLP-C) levels. The lipoprotein profiles of type II diabetic patients (T2DM) (n=194) were measured by an anion-exchange liquid chromatography (AEX-HPLC) and an AGE with lipid-staining or cholesterol-staining.
  
  
  RESULTS
  IDL- and VLDL-cholesterol by the AEX-HPLC, and VLDL-levels by the AGE with lipid-staining and with cholesterol-staining were significantly different in the three groups. In all the subjects, IDL-cholesterol (r=0.531) and VLDL-cholesterol (r=0.880) by the AEX-HPLC method were strongly correlated with RLP-C, but only VLDL levels were correlated with RLP-C in AGE, respectively.
  
  
  CONCLUSIONS
  These results suggest that the AEX-HPLC, which can provide cholesterol levels of not only VLDL but also IDL, is helpful for estimation of lipid profiles in T2DM with high RLP-C.
authors.affiliation: Department of Laboratory Medicine, Jikei University Kashiwa Hospital, 163-1 Kashiwashita Kashiwa, Chiba 277-8567, Japan. hyoshida@jikei.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hirowatari
authors.forename: Hideo
authors.initials: H
authors.lastname: Kurosawa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Manita
authors.forename: Hidekatsu
authors.initials: H
authors.lastname: Yanai
authors.forename: Kumie
authors.initials: K
authors.lastname: Ito
authors.forename: Norio
authors.initials: N
authors.lastname: Tada
mesh_terms: D000368:Aged; D000837:Anion Exchange Resins; D015415:Biomarkers / Q000097:blood; D002784:Cholesterol / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007564:Japan; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood*
chemical_list: D000837:Anion Exchange Resins; D015415:Biomarkers; D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D008074:Lipoproteins; D014280:Triglycerides; C112017:remnant-like particle cholesterol; D002784:Cholesterol