title: [Destruction of Walker-256 tumor vasculature by a novel ultrasound cavitation technique].
abstract: OBJECTIVE
  To explore the feasibility of disrupting tumor microcirculation by the cavitation of microbubbles enhanced ultrasound (US) and analyze its pathological mechanism.
  
  
  METHODS
  Twenty-four SD male rats with subcutaneously transplanted Walker-256 tumor were divided into 3 groups, i.e. ultrasound plus microbubbles group (US + MB), US group and sham group. Pulsed US was delivered to tumor for 3 minutes during an intravenous infusion of microbubbles at 0.2 ml/kg in the US + MB group. The control groups received only the US exposure or the MB injection. Tumor perfusion was visualized with contrast enhanced ultrasound before and 0 min after treatment. Finally the pathological examination was performed.
  
  
  RESULTS
  The contrast perfusion of Walker-256 tumors vanished immediately after treatment in the US + MB group and the gray scale value (GSV) decreased from 121 ± 12 (pre-treatment) to 81 ± 9 (post-treatment, P < 0.01). There was no significant difference of GSV before and after treatment in two control groups (P > 0.05). The GSV values were 112 ± 14 and 111 ± 12 pre-treatment and 113 ± 14 and 103 ± 13 post-treatment in the sham and US groups. The pathological examination showed remarkable hemorrhage, endothelial injuries, increased intercellular edema and in situ thrombosis.
  
  
  CONCLUSIONS
  Microbubble-enhanced ultrasound can significantly disrupt tumor vasculature and block its circulation. And it may become a novel physical anti-angiogenetic therapy for tumor.
authors.affiliation: Department of Ultrasound, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhong
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Zhu
authors.forename: Shun-Ji
authors.initials: SJ
authors.lastname: Gao
authors.forename: Wen-Hong
authors.initials: WH
authors.lastname: Gao
authors.forename: Lu
authors.initials: L
authors.lastname: Qiao
authors.forename: Sheng-Zheng
authors.initials: SZ
authors.lastname: Wu
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Kai-Bin
authors.initials: KB
authors.lastname: Tan
mesh_terms: D000818:Animals; D002279:Carcinoma 256, Walker / Q000098:blood supply* / Q000473:pathology; D008297:Male; D045423:Microbubbles; D009389:Neovascularization, Pathologic / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014464:Ultrasonic Therapy / Q000379:methods*