title: Microbial synthesis of rhamnolipids by Pseudomonas aeruginosa (ATCC 10145) on waste frying oil as low cost carbon source.
abstract: Vegetable edible oils and fats are mainly used for frying purposes in households and the food industry. The oil undergoes degradation during frying and hence has to be replaced from time to time. Rhamnolipids are produced by microbial cultivation using refined vegetable oils as a carbon source and Pseudomonas aeruginosa (ATCC 10145). The raw material cost accounts for 10-30% of the overall cost of biosurfactant production and can be reduced by using low-cost substrates. In this research, attention was focused on the preparation of rhamnolipids, which are biosurfactants, using potential frying edible oils as a carbon source via a microbial fermentation technique. The use of low-cost substrates as a carbon source was emphasized to tilt the cost of production for rhamnolipids. The yield was 2.8 g/L and 7.5 g/L from waste frying oil before and after activated earth treatment, respectively. The crude product contained mainly dirhamnolipids, confirmed by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), liquid chromatography-mass spectroscopy (LC-MS), and (1)H-nuclear magnetic resonance (NMR). Hence, the treatment can be used to convert waste frying oil as a low-cost substrate into a cost-effective carbon source.
authors.affiliation: Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology (University under Section 3 of UGC Act 1956; Formerly UDCT/UICT), Matunga (East), Mumbai, India.
authors.forename: S D
authors.initials: SD
authors.lastname: Wadekar
authors.forename: S B
authors.initials: SB
authors.lastname: Kale
authors.forename: A M
authors.initials: AM
authors.lastname: Lali
authors.forename: D N
authors.initials: DN
authors.lastname: Bhowmick
authors.forename: A P
authors.initials: AP
authors.lastname: Pratap
mesh_terms: D001673:Biodegradation, Environmental; D002244:Carbon / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005285:Fermentation; D006017:Glycolipids / Q000096:biosynthesis*; D006368:Helianthus / Q000737:chemistry; D006358:Hot Temperature; D019787:Linoleic Acid / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010545:Peroxides / Q000032:analysis / Q000737:chemistry; D010938:Plant Oils / Q000032:analysis / Q000737:chemistry*; D011312:Pressure; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000254:growth & development; D000074242:Sunflower Oil; D013500:Surface Tension; D013501:Surface-Active Agents / Q000737:chemistry; D013997:Time Factors; D014783:Viscosity; D014866:Waste Products / Q000032:analysis
chemical_list: D006017:Glycolipids; D010545:Peroxides; D010938:Plant Oils; D000074242:Sunflower Oil; D013501:Surface-Active Agents; D014866:Waste Products; C418382:rhamnolipid; D002244:Carbon; D019787:Linoleic Acid