title: Drosophila chibby is required for basal body formation and ciliogenesis but not for Wg signaling.
abstract: Centriole-to-basal body conversion, a complex process essential for ciliogenesis, involves the progressive addition of specific proteins to centrioles. CHIBBY (CBY) is a coiled-coil domain protein first described as interacting with β-catenin and involved in Wg-Int (WNT) signaling. We found that, in Drosophila melanogaster, CBY was exclusively expressed in cells that require functional basal bodies, i.e., sensory neurons and male germ cells. CBY was associated with the basal body transition zone (TZ) in these two cell types. Inactivation of cby led to defects in sensory transduction and in spermatogenesis. Loss of CBY resulted in altered ciliary trafficking into neuronal cilia, irregular deposition of proteins on spermatocyte basal bodies, and, consequently, distorted axonemal assembly. Importantly, cby(1/1) flies did not show Wingless signaling defects. Hence, CBY is essential for normal basal body structure and function in Drosophila, potentially through effects on the TZ. The function of CBY in WNT signaling in vertebrates has either been acquired during vertebrate evolution or lost in Drosophila.
authors.affiliation: Centre de Génétique et de Physiologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique UMR 5534, Université Claude Bernard Lyon 1, Villeurbanne, Lyon F69622, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Enjolras
authors.forename: Joëlle
authors.initials: J
authors.lastname: Thomas
authors.forename: Brigitte
authors.initials: B
authors.lastname: Chhin
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Cortier
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Duteyrat
authors.forename: Fabien
authors.initials: F
authors.lastname: Soulavie
authors.forename: Maurice J
authors.initials: MJ
authors.lastname: Kernan
authors.forename: Anne
authors.initials: A
authors.lastname: Laurençon
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Durand
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002502:Centrioles / Q000378:metabolism; D002923:Cilia / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D007248:Infertility, Male; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D000071701:Regulatory Factor X Transcription Factors; D011984:Sensory Receptor Cells / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D060449:Wnt Signaling Pathway; D051155:Wnt1 Protein / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; C473749:Cby protein, Drosophila; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D051155:Wnt1 Protein; C074622:wg protein, Drosophila