title: Osteogenic potential of human umbilical cord-derived mesenchymal stromal cells cultured with umbilical cord blood-derived autoserum.
abstract: OBJECTIVE
  Osteogenesis in the bone defect at the site of an alveolar cleft is important to enable patients with cleft lip and palate to acquire dental articulation. The presence of umbilical cord-derived mesenchymal stem cells has been reported. In this study, we used autoserum derived from the umbilical cord blood (UCB) of neonates in an attempt to examine the osteoblastic differentiation potential of umbilical cord-derived mesenchymal stromal cells (UC-MSCs) in nude mice.
  
  
  METHODS
  UCB, hydroxyapatite, and rhBMP were used as the supply source of autoserum, scaffold, and osteoinductive growth factor, respectively. MSCs, obtained from Wharton's jelly and cultured for 3-4weeks to induce their differentiation into osteoblasts, were implanted subcutaneously into the dorsum of male nude mice for 6weeks before the assessment by real-time reverse transcriptase chain reaction of osteoblast marker expression.
  
  
  RESULTS
  UCB-derived autoserum was a viable source for the culture and implantation of UC-MSCs. The osteoblastic differentiation potential of UC-MSCs was demonstrated in nude mice by performing immunohistochemical staining and by the presence of osteoblast marker expression.
  
  
  CONCLUSIONS
  Our results confirm the osteogenic potential of UC-MSCs and provide basic evidence for the realization of regenerative medicine using autologous tissues.
authors.affiliation: Department of Plastic and Aesthetic Surgery, School of Medicine, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan. kyokobaba@med.kitasato-u.ac.jp
authors.forename: Kyoko
authors.initials: K
authors.lastname: Baba
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Ikemoto
authors.forename: Kazuya
authors.initials: K
authors.lastname: Aoyagi
authors.forename: Akira
authors.initials: A
authors.lastname: Takeda
authors.forename: Eiju
authors.initials: E
authors.lastname: Uchinuma
mesh_terms: D017667:Adipocytes / Q000502:physiology; D050156:Adipogenesis / Q000502:physiology; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000880:Anthraquinones; D001391:Azo Compounds; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D017886:Durapatite / Q000737:chemistry; D005312:Fetal Blood / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D010006:Osteoblasts / Q000502:physiology*; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000502:physiology*; D053657:Platelet-Rich Plasma / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000737:chemistry; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D000880:Anthraquinones; D001391:Azo Compounds; D055396:Bone Morphogenetic Protein 2; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit; C493709:RUNX2 protein, human; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D015675:Osteocalcin; C004468:Alizarin Red S; D017886:Durapatite; D000469:Alkaline Phosphatase; C011049:oil red O