title: Simple and low-cost high-performance liquid chromatographic method for determination of D- and L-amino acids.
abstract: In this article, a simple and low-cost method for the analysis of amino acid enantiomers by using high-performance liquid chromatography (HPLC) is described. In this method, the amino acids are modified to diastereomers in order to be separated into enantiomers on a usual C(18) reversed-phase column. Methanol instead of acetonitrile is used as an elution solvent; the results of HPLC with methanol elution are comparable with those of HPLC with acetonitrile elution. Sub-nanomolar sensitivity is attained by measuring the absorbance at 340 nm in analysis of 15 amino-acid enantiomers.
authors.affiliation: Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Kanda-Surugadai, Chiyoda-Word, Tokyo 101-8308, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Tojo
authors.forename: Masahito
authors.initials: M
authors.lastname: Murakami
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nagata
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000596:Amino Acids / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000191:economics / Q000379:methods*; D005260:Female; D008297:Male; D000432:Methanol / Q000737:chemistry; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
chemical_list: D000097:Acetonitriles; D000596:Amino Acids; D000432:Methanol; C032159:acetonitrile