title: New photolabile BAPTA-based Ca2+ cages with improved photorelease.
abstract: The efficient synthesis, physicochemical and photolytical properties of a photoactivable BAPTA-based Ca(2+) cage containing two photosensitive o-nitrobenzhydryl groups attached to the aromatic core are described. Ca(2+) release in living cells was evaluated. The double substitution with the chromophores caused a significant improvement of the Ca(2+) release properties of nitr-T versus singly substituted reported nitr-x derivatives without compromising Ca(2+)/Mg(2+) selectivity or pH insensitivity. Our results demonstrate a general strategy to improve light-triggered Ca(2+) release which may result in more efficient, selective, and pH-insensitive photolabile Ca(2+) chelators.
authors.affiliation: Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany.
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Cui
authors.forename: Radu A
authors.initials: RA
authors.lastname: Gropeanu
authors.forename: David R
authors.initials: DR
authors.lastname: Stevens
authors.forename: Jens
authors.initials: J
authors.lastname: Rettig
authors.forename: Aránzazu
authors.initials: A
authors.lastname: del Campo
mesh_terms: D000818:Animals; D002118:Calcium / Q000008:administration & dosage* / Q000378:metabolism; D002478:Cells, Cultured; D002614:Chelating Agents / Q000737:chemistry*; D019439:Chromaffin Cells / Q000166:cytology / Q000378:metabolism; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D008027:Light; D051379:Mice; D018408:Patch-Clamp Techniques; D010782:Photolysis
chemical_list: D002614:Chelating Agents; D003692:Delayed-Action Preparations; D004533:Egtazic Acid; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium