title: "tet(U)" is not a tetracycline resistance determinant.
abstract: The enterococcal plasmid pKQ10 has been reported to carry a poorly characterized tetracycline resistance determinant designated tet(U). However, in a series of studies intended to further characterize this determinant, we have been unable to substantiate the claim that tet(U) confers resistance to tetracyclines. In line with these results, bioinformatic analysis provides compelling evidence that "tet(U)" is in fact the misannotated 3' end of a gene encoding a rolling-circle replication initiator (Rep) protein.
authors.affiliation: Antimicrobial Research Centre and Institute of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.
authors.forename: Jamie A
authors.initials: JA
authors.lastname: Caryl
authors.forename: Georgina
authors.initials: G
authors.lastname: Cox
authors.forename: Stefan
authors.initials: S
authors.lastname: Trimble
authors.forename: Alex J
authors.initials: AJ
authors.lastname: O'Neill
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D019295:Computational Biology; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics*; D013754:Tetracyclines / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D013754:Tetracyclines