title: Recombinant Fc-IL-18BPc isoform inhibits IL-18-induced cytokine production.
abstract: IL-18 is a pro-inflammatory cytokine that is produced from T cells and NK cells. IL-18 has been implicated in the pathogenesis of various inflammatory and cardiovascular diseases. IL-18 binding protein (IL-18BP) is a natural inhibitor of IL-18 that possesses higher affinity to IL-18 than that of the IL-18 receptor alpha chain on the cell surface. Human isoform a and c among four isoforms of IL-18BPs have an inhibitory effect on IL-18-induced cytokines whereas mouse IL-18BP isoforms exist only in two isoforms: c and d. Fc-fusion protein is a molecule in which the immunoglobulin Fc is fused genetically to a protein of interest, such as an extracellular domain of a receptor, ligand, or enzyme. In this study, we expressed and purified human Fc-IL-18BPa and c isoforms from CHO-DG44 cells and their biological activities were compared to each other. This is the first time that expressed recombinant human Fc-IL-18BPc has been examined for its biological activity on IL-18-induced IFNγ in human PBMC and IL-6 in A549/IL-18Rβ.
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Sciences and Technology, Konkuk University Medical Center, School of Medicine, Konkuk University, Seoul, Korea.
authors.forename: Kwangwon
authors.initials: K
authors.lastname: Hong
authors.forename: Kwangjun
authors.initials: K
authors.lastname: Oh
authors.forename: Siyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Hong
authors.forename: Jida
authors.initials: J
authors.lastname: Choi
authors.forename: Areum
authors.initials: A
authors.lastname: Kwak
authors.forename: Dongjun
authors.initials: D
authors.lastname: Kang
authors.forename: Eunsom
authors.initials: E
authors.lastname: Kim
authors.forename: Seunghyun
authors.initials: S
authors.lastname: Jo
authors.forename: Hyunjhung
authors.initials: H
authors.lastname: Jhun
authors.forename: Soohyun
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000378:metabolism; D020382:Interleukin-18 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism*; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D007141:Immunoglobulin Fc Fragments; D036341:Intercellular Signaling Peptides and Proteins; D020382:Interleukin-18; D015850:Interleukin-6; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; C118370:interleukin-18 binding protein; D007371:Interferon-gamma