title: Anti-inflammatory activity of lactobacillus on carrageenan-induced paw edema in male wistar rats.
abstract: Introduction. Lactobacillus casei and Lactobacillus acidophilus were used to assess the anti-inflammatory properties in carrageenan induced acute inflammatory model. Materials and Methods. Diclofenac sodium was used as standard drug at concentration of 150 mg/kg of body weight. Culture of Lactobacillus  2 × 10(7) CFU/ml was given orally. Edema was induced with 1% carrageenan to all the groups after one hour of the oral treatments. Paw thickness was checked at t = 1, 2, 3, 4, 5, and 24 hours. Stair climbing score and motility score were assessed at t = 24 hours. Cytokines assay for IL-6, IL-10, and TNF-α was performed on serum samples. Results. Lactobacillus showed a statistically significant decrease in paw thickness at P < 0.001. L. acidophilus and L. casei decreased by 32% and 28% in paw thickness. They both significantly increased the stair climbing and motility score. Lactobacillus treatment significantly downregulated IL-6 and TNF-α while upregulated IL-10 at P < 0.0001. Conclusion. L. casei and L. acidophilus significantly decreased the inflammatory reactions induced by carrageenan. This study has also proposed that Lactobacillus ameliorated the inflammatory reaction by downregulating the proinflammatory cytokines pathway.
authors.affiliation: Department of Microbiology, Barkatullah University, Bhopal 462026, India.
authors.forename: Sarika
authors.initials: S
authors.lastname: Amdekar
authors.forename: Purabi
authors.initials: P
authors.lastname: Roy
authors.forename: Vinod
authors.initials: V
authors.lastname: Singh
authors.forename: Avnish
authors.initials: A
authors.lastname: Kumar
authors.forename: Rambir
authors.initials: R
authors.lastname: Singh
authors.forename: Poonam
authors.initials: P
authors.lastname: Sharma