title: Rapid and simple DNA extraction method for the detection of enterotoxigenic Staphylococcus aureus directly from food samples: comparison of PCR and LAMP methods.
abstract: OBJECTIVE
  The study describes the development of simple and rapid DNA extraction method in combination with loop-mediated isothermal amplification (LAMP) to detect enterotoxigenic Staphylococcus aureus in food samples.
  
  
  RESULTS
  In this study, isolation of genomic DNA of enterotoxigenic Staph. aureus from spiked milk, milk burfi, khoa, sugarcane juice and boiled rice was carried out by boiling the isolated sample pellets for 10 min with 1% Triton X-100. The isolated DNA was evaluated by polymerase chain reaction (PCR) and LAMP method. The LAMP was found to be 100 times more sensitive than PCR. The LAMP assay was very specific for Staph. aureus, and the presence of other contaminating bacterial DNAs and food matrix did not interfere or inhibit the LAMP assay.
  
  
  CONCLUSIONS
  The template DNA extraction method developed in this study for food samples is simple, rapid and cost-effective. LAMP was found to be less sensitive to matrix effect of food, compared to PCR.
  
  
  CONCLUSIONS
  The method is suitable for direct detection of Staph. aureus without any enrichment in contaminated food samples and hence finds its application in food safety analysis, in permutation with LAMP.
authors.affiliation: Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute (CSIR), Mysore, Karnataka, India.
authors.forename: N
authors.initials: N
authors.lastname: Sowmya
authors.forename: M S
authors.initials: MS
authors.lastname: Thakur
authors.forename: H K
authors.initials: HK
authors.lastname: Manonmani
mesh_terms: D000818:Animals; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005516:Food Microbiology / Q000379:methods*; D008892:Milk / Q000382:microbiology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D017830:Octoxynol; D012275:Oryza / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D031786:Saccharum / Q000382:microbiology; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D017830:Octoxynol