title: Lactobacillus casei extract induces apoptosis in gastric cancer by inhibiting NF-κB and mTOR-mediated signaling.
abstract: Lactobacillus casei extract (LBX) has been reported to prevent gastric cancer, but the underlying mechanism remains unclear. The proliferation and cell death of gastric cancer KATO3 cells were examined after treatment with LBX for various times and at various doses. LBX inhibited the growth of gastric cancer cells and induced apoptosis by inactivating NF-κB promoter activity. Apoptosis induced by LBX, however, is not directly associated with the intrinsic mitochondrial pathway. Immunoblot analysis revealed that LBX decreased the expressions of NF-κB and IκB. The reduced NF-κB levels led to the decreased phosphorylation of mTOR signaling components, such as PI3K, Akt, and (p70)S6 kinase. These results showed for the first time that LBX induced apoptosis in gastric cancer cells by inhibiting NF-κB and mTOR-mediated signaling.
authors.affiliation: Korea Basic Science Institute, Daejeon, Republic of Korea.
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Hwang
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Baek
authors.forename: Kyeong Eun
authors.initials: KE
authors.lastname: Yang
authors.forename: Hwa-Seung
authors.initials: HS
authors.lastname: Yoo
authors.forename: Chong-Kwan
authors.initials: CK
authors.lastname: Cho
authors.forename: Yeon-Weol
authors.initials: YW
authors.lastname: Lee
authors.forename: Junsoo
authors.initials: J
authors.lastname: Park
authors.forename: Chi-Yong
authors.initials: CY
authors.lastname: Eom
authors.forename: Zee-Won
authors.initials: ZW
authors.lastname: Lee
authors.forename: Jong-Soon
authors.initials: JS
authors.lastname: Choi
authors.forename: Ik-Soon
authors.initials: IS
authors.lastname: Jang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001688:Biological Products / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000737:chemistry*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011401:Promoter Regions, Genetic / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D001688:Biological Products; D020672:I-kappa B Proteins; D016328:NF-kappa B; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D058570:TOR Serine-Threonine Kinases; C546842:MTOR protein, human