title: [Research on population structure and distribution characteristic of indigenous microorganism in post-polymer-flooding oil reservoir].
abstract: Denaturing gradient gel electrophoresis (DGGE) method and principal component analysis (PCA) method were used to analyze the structures of microorganism population in injection wells and production wells of a post-polymer-flooding oil reservoir in Daqing oil field. The results showed that the dominant species in injection wellhead were aerobic bacteria Pseudomonas and Acinenobacter. Facultative anaerobic bacteria Enterbacter was the dominant bacteria in near area of injection wells. Bacteria detected in production wells included Thauera, Clostridia, Pseudomonas, Petrobacter and some uncultured bacteria. Methanosaeta turned out to be the only archaea detected in injection wells, which was an aceticlastic methane-producing archaeon. Archaea detected in production wells consisted of Methanomicrobium, Methanospirillum and Methanobacterium. In general, aerobic bacteria, facultative anaerobe, and strictly anaerobic bacteria distributed successively from injection wells to production wells in this block. The dominant populations of archaea were different between injection wells and production wells, while were influenced by different environments and microbial metabolism products.
authors.affiliation: Key Laboratory of Molecular Microbiology Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. mulanmeor@gmail.com
authors.forename: Ling-Xia
authors.initials: LX
authors.lastname: Zhao
authors.forename: Pei-Ke
authors.initials: PK
authors.lastname: Gao
authors.forename: Mei-Na
authors.initials: MN
authors.lastname: Cao
authors.forename: Meng-Li
authors.initials: ML
authors.lastname: Gao
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Li
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Zhu
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
mesh_terms: D000150:Acinetobacter / Q000302:isolation & purification; D001105:Archaea / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D061329:Oil and Gas Fields / Q000382:microbiology*; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D011108:Polymers; D025341:Principal Component Analysis; D011549:Pseudomonas / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060986:Water Wells / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D010578:Petroleum; D011108:Polymers; D012336:RNA, Ribosomal, 16S