title: Manufacture and properties of bifidogenic saccharides derived from wood mannan.
abstract: Pinus pinaster  wood samples were subjected to double hydrothermal processing. The liquors coming from the second stage, containing soluble saccharides of polymeric or oligomeric nature from hemicelluloses (POHs), were subjected to membrane processing (operating in discontinuous diafiltration) for refining and fractionation. Refined POH fractions were characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry and chromatographic techniques. The most complex POH component was made up of 14 hexoses and contained 4 acetyl groups. The fermentability of purified POHs by human fecal inocula was assessed by measuring both carbon source consumption and formation of short-chain fatty acids. The bifidogenic ability of POHs was confirmed by fluorescence in situ hybridization. The stimulatory effects on the bifidobacterial population reached by POHs were of the same order as those obtained with commercial fructooligosaccharides.
authors.affiliation: Department of Chemical Engineering, Faculty of Science, University of Vigo-Campus Ourense, As Lagoas, 32004 Ourense, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Rivas
authors.forename: Beatriz
authors.initials: B
authors.lastname: Gullón
authors.forename: Patricia
authors.initials: P
authors.lastname: Gullón
authors.forename: José Luis
authors.initials: JL
authors.lastname: Alonso
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Parajó
mesh_terms: D001644:Bifidobacterium / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D006801:Humans; D006868:Hydrolysis; D008351:Mannans / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D028223:Pinus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D056692:Prebiotics; D014934:Wood / Q000737:chemistry*
chemical_list: D008351:Mannans; D009844:Oligosaccharides; D011134:Polysaccharides; D056692:Prebiotics; C007916:hemicellulose