title: EhADH112 is a Bro1 domain-containing protein involved in the Entamoeba histolytica multivesicular bodies pathway.
abstract: EhADH112 is an Entamoeba histolytica Bro1 domain-containing protein, structurally related to mammalian ALIX and yeast BRO1, both involved in the Endosomal Sorting Complexes Required for Transport (ESCRT)-mediated multivesicular bodies (MVB) biogenesis. Here, we investigated an alternative role for EhADH112 in the MVB protein trafficking pathway by overexpressing 166 amino acids of its N-terminal Bro1 domain in trophozoites. Trophozoites displayed diminished phagocytosis rates and accumulated exogenous Bro1 at cytoplasmic vesicles which aggregated into aberrant complexes at late stages of phagocytosis, probably preventing EhADH112 function. Additionally, the existence of a putative E. histolytica ESCRT-III subunit (EhVps32) presumably interacting with EhADH112, led us to perform pull-down experiments with GST-EhVps32 and [(35)S]-labeled EhADH112 or EhADH112 derivatives, confirming EhVps32 binding to EhADH112 through its Bro1 domain. Our overall results define EhADH112 as a novel member of ESCRT-accessory proteins transiently present at cellular surface and endosomal compartments, probably contributing to MVB formation during phagocytosis.
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México, DF, Mexico.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Bañuelos
authors.forename: Guillermina
authors.initials: G
authors.lastname: García-Rivera
authors.forename: Israel
authors.initials: I
authors.lastname: López-Reyes
authors.forename: Leobardo
authors.initials: L
authors.lastname: Mendoza
authors.forename: Arturo
authors.initials: A
authors.lastname: González-Robles
authors.forename: Silvia
authors.initials: S
authors.lastname: Herranz
authors.forename: Olivier
authors.initials: O
authors.lastname: Vincent
authors.forename: Esther
authors.initials: E
authors.lastname: Orozco
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000737:chemistry; D018797:Cell Cycle Proteins / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D056827:Endosomal Sorting Complexes Required for Transport / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004748:Entamoeba histolytica / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D008958:Models, Molecular; D010587:Phagocytosis / Q000502:physiology; D015800:Protozoan Proteins / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D053058:Trophozoites / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; C480237:Bro1 protein, S cerevisiae; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; C122005:EhADH112 protein, Entamoeba histolytica; D056827:Endosomal Sorting Complexes Required for Transport; C117020:PDCD6IP protein, human; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins