title: A novel osteogenesis technique: The expansible guided bone regeneration.
abstract: Guided bone regeneration is a unique osteogenesis technique that requires a barrier membrane under periosteum to create space for bone regeneration. However, creating sizeable spaces is clinically not commonly feasible. A titanium plate and a thin silicone membrane were surgically layered on each calvaria of eight rabbits. Then, the periphery of the silicone membrane was fixed by a plastic ring to the underlying bone using titanium micro screws. After 1 week, a 5-mm-length titanium screw was used to elevate the titanium plate, which in turn elevated the silicone membrane together with overlying soft tissue in a rate of 1 mm/day for 5 days to create a secluded space. Animals were killed at 2 months (n = 4, group 1) and 4 months (n = 4, group 2) after the elevation. Histological and microradiographical analyses demonstrated creation of an amount of de novo bone formation (68.2 ± 22 mm(3) in group 1 and 70.3 ± 14 mm(3) in group 2) in the sizeable created spaces (207.1 ± 31 mm(3) in group 1 and 202 ± 21 mm(3) in group 2) without exposure of the device. This novel osteogenesis technique, "expansible guided bone regeneration," created a substantial in vivo incubator without applying growth factors or osteoprogenitor cells. Creating a growing space over the secluded surface allowed the development of normal biological healing process occurring on the bone surface into a regenerative process, generating bone outside the genetically determined skeletal bone. This technique is a new tissue engineering approach stimulating endogenous tissue repair without applying cells or factors exogenously.
authors.affiliation: Department of Oral Implantology and Regenerative Dental Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Osama
authors.initials: O
authors.lastname: Zakaria
authors.forename: Marwa
authors.initials: M
authors.lastname: Madi
authors.forename: Shohei
authors.initials: S
authors.lastname: Kasugai