title: [HPLC fingerprint spectrum of xinjiang uyghur medicine Nigella glandulifera seed].
abstract: OBJECTIVE
  To establish the HPLC fingerprint spectrum of Nigella glandulifera seed identification.
  
  
  METHODS
  The chromatographic system consisted of a Shim-pack ODS C18 column (150 mm x 4.6 mm, 5 microm) and a mobile phase of acetonitrile-0.1% formic acid in condition of gradient elution,The detection wavelength was 280 nm. The column temperature was 30 degrees C.
  
  
  RESULTS
  Detected the HPLC fingerprint spectrum of 10 batches samples Nigella glandulifera seed from Xinjiang; Calibrated 18 characteristic peaks. The result was in accordance with the technical requirement of fingerprint spectrum. The similarity calculation software showed that the similarity was higher than 0. 95.
  
  
  CONCLUSIONS
  The HPLC fingerprint spectrum of Nigella glandulifera seed can be used as an identification method. It may provide the basis for quality control of Nigella glandulifera seed.
authors.affiliation: College of Pharmacy, Xinjiang Medical University, Urmqi 830054, China. txf717@163.com
authors.forename: Xiu-Fang
authors.initials: XF
authors.lastname: Tan
authors.forename: Xiao-Jin
authors.initials: XJ
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Shi
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cao
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D031844:Nigella / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D012997:Solvents