title: Detection of cells captured with antigens on shear horizontal surface-acoustic-wave sensors.
abstract: Techniques to separate cells are widely applied in immunology. The technique to separate a specific antigen on a microfluidic platform involves the use of a shear horizontal surface-acoustic-wave (SH-SAW) sensor. With specific antibodies conjugated onto the surface of the SH-SAW sensors, this technique can serve to identify specific cells in bodily fluids. Jurkat cells, used as a target in this work, provide a model of cells in small abundance (1:1000) for isolation and purification with the ultimate goal of targeting even more dilute cells. T cells were separated from a mixed-cell medium on a chip (Jurkat cells/K562 cells, 1/1000). A novel microchamber was developed to capture cells during the purification, which required a large biosample. Cell detection was demonstrated through the performance of genetic identification on the chip.
authors.affiliation: Institute of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: Hsu-Chao
authors.initials: HC
authors.lastname: Hao
authors.forename: Hwan-You
authors.initials: HY
authors.lastname: Chang
authors.forename: Tsung-Pao
authors.initials: TP
authors.lastname: Wang
authors.forename: Da-Jeng
authors.initials: DJ
authors.lastname: Yao
mesh_terms: D000162:Acoustics / Q000295:instrumentation*; D000906:Antibodies / Q000378:metabolism; D000941:Antigens / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002477:Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D061848:Optical Imaging; D011437:Propylamines; D033081:Shear Strength; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D000906:Antibodies; D000941:Antigens; D011437:Propylamines; D012821:Silanes; D014867:Water; D012822:Silicon Dioxide; C477625:amino-propyl-triethoxysilane