title: [Molecular test Genotype® MTBDRplus, an alternative to rapid detection of multidrug resistance tuberculosis].
abstract: The Genotype®MTBDRplus molecular test is a method that allows identification of the most frequent mutations associated with resistance to major first-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RFP). The aim of this study was to evaluate the performance of the molecular test with culture and smear- positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profiles previously determined by the reference method "Agar Plate Proportions" (APP). The molecular test from cultures showed a sensitivity of 100 %, 97,5 % and 96,97 % for RIF, INH and MDR respectively while from sputums the sensitivity was 95,65 %, 96,77 % and 95,24 % for RIF, INH and MDR respectively. We conclude that the molecular test Genotype®MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.
authors.affiliation: Laboratorio de Referencia Nacional de Micobacterias, Instituto Nacional de Salud, Lima, Perú.
authors.forename: Luis
authors.initials: L
authors.lastname: Asencios
authors.forename: Marco
authors.initials: M
authors.lastname: Galarza
authors.forename: Neyda
authors.initials: N
authors.lastname: Quispe
authors.forename: Lucy
authors.initials: L
authors.lastname: Vásquez
authors.forename: Elena
authors.initials: E
authors.lastname: Leo
authors.forename: Eddy
authors.initials: E
authors.lastname: Valencia
authors.forename: Juan
authors.initials: J
authors.lastname: Ramírez
authors.forename: Margoth
authors.initials: M
authors.lastname: Acurio
authors.forename: Rosario
authors.initials: R
authors.lastname: Salazar
authors.forename: Alberto
authors.initials: A
authors.lastname: Mendoza-Ticona
authors.forename: Omar
authors.initials: O
authors.lastname: Cáceres
mesh_terms: D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000494:pharmacology*; D005838:Genotype; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D025202:Molecular Diagnostic Techniques / Q000379:methods; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology*; D013997:Time Factors; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D000904:Antibiotics, Antitubercular; D000995:Antitubercular Agents; D007538:Isoniazid; D012293:Rifampin