title: Tumor microenvironmental genomic alterations in juvenile nasopharyngeal angiofibroma.
abstract: BACKGROUND
  To better characterize the pathophysiology of juvenile nasopharyngeal angiofibroma (JNA), endothelial and stromal cells were evaluated by genomic imbalances in association with transcript expression levels of genes mapped on these altered regions.
  
  
  METHODS
  High-resolution comparative genomic hybridization (HR-CGH) was used in laser-captured endothelial and stromal cells from 9 JNAs. Ten genes were evaluated by quantitative real-timereverse transcription polymerase chain reaction (qRT-PCR) in 15 cases.
  
  
  RESULTS
  Although gains were more frequently detected in endothelial cells, 57% of chromosomal alterations were common by both components. Gene expression analyses revealed a positive correlation between endothelial and stromal components for ASPM, CDH1, CTNNB1, FGF18, and SUPT16H. A significant difference was found for FGF18 and AURKB overexpression in stromal cells and AR down-expression in endothelial cells.
  
  
  CONCLUSIONS
  A similar pattern of gene expression and chromosomal imbalances in both exponents would suggest a common mechanism of functional regulation. AURKB, FGF18, and SUPT16H were identified as potential molecular markers in JNA.
authors.affiliation: NeoGene Laboratory, Cancer and Treatment Research Center, Antonio Prudente Foundation, AC Camargo Hospital, São Paulo, SP, Brazil.
authors.forename: Sara Martoreli
authors.initials: SM
authors.lastname: Silveira
authors.forename: Maria Aparecida
authors.initials: MA
authors.lastname: Custódio Domingues
authors.forename: Ossamu
authors.initials: O
authors.lastname: Butugan
authors.forename: Maria Mitzi
authors.initials: MM
authors.lastname: Brentani
authors.forename: Silvia Regina
authors.initials: SR
authors.lastname: Rogatto
mesh_terms: D000293:Adolescent; D018322:Angiofibroma / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization / Q000379:methods; D016001:Confidence Intervals; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D020022:Genetic Predisposition to Disease; D042822:Genomic Instability; D006801:Humans; D008297:Male; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012494:Sampling Studies; D016610:Tissue Embedding; D059016:Tumor Microenvironment / Q000235:genetics*; D055815:Young Adult