title: Hypolipidemic effects of a new piperine derivative GB-N from Piper longum in high-fat diet-fed rats.
abstract: BACKGROUND
  Long pepper, Piper longum Linn. (Piperaceae), is widely used in traditional Mongolian medicine for treating hyperlipidemia and coronary heart disease.
  
  
  OBJECTIVE
  To investigate the hypolipidemic effects of a new piperine derivative GB-N isolated from long pepper in high-fat diet-fed rats.
  
  
  METHODS
  The levels of serum total cholesterol, triacylglycerols (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were determined by enzymatic colorimetric method. The levels of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA), CYP7A1, lecithin cholesterol acyltransferase (LCAT) and LDL receptor (LDLR) mRNA and protein expression were detected by real-time polymerase chain reaction and western blot analysis.
  
  
  CONCLUSIONS
  Compared with model rats, oral administration of GB-N at doses of 2.5-10 mg/kg to hyperlipidemic rats could significantly decrease the levels of serum TG from 1.54 mmol/L in hyperlipidemic rats to 0.94-1.02 mmol/L, with an increase in serum HDL-C levels from 0.40 mmol/L in hyperlipidemic rats to 1.21-2.26 mmol/L. Treatment with GB-N (10 mg/kg) could also significantly upregulate levels of hepatic HMG-CoA reductase, CYP7A1, LCAT and LDLR mRNA and protein expression.
  
  
  CONCLUSIONS
  GB-N had hypolipidemic activity via regulating lipid metabolism pathways in liver of hyperlipidemic rats and could be explored as a potential agent for the prevention of hyperlipidemia diseases.
authors.affiliation: Institute of Macromolecular Chemistry and Mongolian Medicine, Inner Mongolia University, Huhhot 010021, Inner Mongolia, China.
authors.forename: Lidao
authors.initials: L
authors.lastname: Bao
authors.forename: Shimin
authors.initials: S
authors.lastname: Bai
authors.forename: Gereltu
authors.initials: G
authors.lastname: Borijihan
mesh_terms: D000577:Amides / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D052117:Benzodioxoles / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D004305:Dose-Response Relationship, Drug; D055808:Drug Discovery; D020834:Ethnopharmacology; D005638:Fruit / Q000737:chemistry*; D006949:Hyperlipidemias / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D015394:Molecular Structure; D008986:Mongolia; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000235:genetics / Q000378:metabolism; D031701:Piper / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000577:Amides; D052117:Benzodioxoles; D000960:Hypolipidemic Agents; C576583:N-isobutyl-5-(3,4-methylenedioxyphenyl)-2,4-pentadienamine; D012333:RNA, Messenger; D011973:Receptors, LDL; C532838:CYP7A1 protein, rat; D002790:Cholesterol 7-alpha-Hydroxylase; D007862:Phosphatidylcholine-Sterol O-Acyltransferase