title: Arenimonas daechungensis sp. nov., isolated from the sediment of a eutrophic reservoir.
abstract: A gram-negative, non-motile, non-spore-forming and rod-shaped bacterial strain, CH15-1(T), was isolated from a sediment sample taken from Daechung Reservoir, South Korea, during the late-blooming period of cyanobacteria. Strain CH15-1(T) grew optimally at pH 7.0 and 30 °C. A phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain CH15-1(T) belongs to the genus Arenimonas with the similarity range from 92.6-97.4 % and is closely related to Arenimonas oryziterrae YC6267(T) (97.4 %), Arenimonas composti TR7-09(T) (95.4 %), Arenimonas metalli CF5-1(T) (94.7 %), Arenimonas malthae CC-JY-1(T) (94.6 %) and Arenimonas donghaensis HO3-R19(T) (92.6 %). However, the DNA-DNA hybridization between strain CH15-1(T) and the closest strain, Arenimonas oryziterrae YC6267(T), was 8.9-12.9 %. The DNA G+C content was 63.9 mol% compared to A. oryziterrae YC626(T), 65.8 mol%. Strain CH15-1(T) included Q-8 as the major ubiquinone and phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmonomethylethanolamine as the major polar lipids. The major fatty acids (>5 %) were iso-C(15 : 0), iso-C(16 : 0), iso-C(14 : 0), iso-C(11 : 0) 3-OH, iso-C(17 : 0) and summed feature 9 (iso-C(17 : 1)ω9c and/or C(16 : 0) 10-methyl). On the basis of phylogenetic, phenotypic and genetic data, strain CH15-1(T) was classified in the genus Arenimonas as a member of a novel species, for which the name Arenimonas daechungensis sp. nov. is proposed. The type strain is CH15-1(T) ( = KCTC 23553(T) = DSM 24763(T)).
authors.affiliation: University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 305-850, Republic of Korea.|Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Hangsak
authors.initials: H
authors.lastname: Huy
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Lee
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Keun Chul
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Chi-Yong
authors.initials: CY
authors.lastname: Ahn
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Hee-Mock
authors.initials: HM
authors.lastname: Oh
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S