title: Deciphering chondrocyte behaviour in matrix-induced autologous chondrocyte implantation to undergo accurate cartilage repair with hyaline matrix.
abstract: Since the emergence in the 1990s of the autologous chondrocytes transplantation (ACT) in the treatment of cartilage defects, the technique, corresponding initially to implantation of chondrocytes, previously isolated and amplified in vitro, under a periosteal membrane, has greatly evolved. Indeed, the first generations of ACT showed their limits, with in particular the dedifferentiation of chondrocytes during the monolayer culture, inducing the synthesis of fibroblastic collagens, notably type I collagen to the detriment of type II collagen. Beyond the clinical aspect with its encouraging results, new biological substitutes must be tested to obtain a hyaline neocartilage. Therefore, the use of differentiated chondrocytes phenotypically stabilized is essential for the success of ACT at medium and long-term. That is why researchers try now to develop more reliable culture techniques, using among others, new types of biomaterials and molecules known for their chondrogenic activity, giving rise to the 4th generation of ACT. Other sources of cells, being able to follow chondrogenesis program, are also studied. The success of the cartilage regenerative medicine is based on the phenotypic status of the chondrocyte and on one of its essential component of the cartilage, type II collagen, the expression of which should be supported without induction of type I collagen. The knowledge accumulated by the scientific community and the experience of the clinicians will certainly allow to relief this technological challenge, which influence besides, the validation of such biological substitutes by the sanitary authorities.
authors.affiliation: Laboratoire matrice extracellulaire et pathologie, IFR Icore 146, UFR de médecine, université de Caen Basse-Normandie, avenue Côte-de-Nacre, 14032 Caen cedex, France. Magali.demoor@unicaen.fr
authors.forename: M
authors.initials: M
authors.lastname: Demoor
authors.forename: L
authors.initials: L
authors.lastname: Maneix
authors.forename: D
authors.initials: D
authors.lastname: Ollitrault
authors.forename: F
authors.initials: F
authors.lastname: Legendre
authors.forename: E
authors.initials: E
authors.lastname: Duval
authors.forename: S
authors.initials: S
authors.lastname: Claus
authors.forename: F
authors.initials: F
authors.lastname: Mallein-Gerin
authors.forename: S
authors.initials: S
authors.lastname: Moslemi
authors.forename: K
authors.initials: K
authors.lastname: Boumediene
authors.forename: P
authors.initials: P
authors.lastname: Galera
mesh_terms: D002356:Cartilage / Q000187:drug effects / Q000502:physiology*; D019902:Chondrocytes / Q000502:physiology* / Q000637:transplantation*; D006801:Humans; D006818:Hyalin / Q000502:physiology; D051457:Hyaline Cartilage / Q000502:physiology; D008954:Models, Biological; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D054457:Tissue Scaffolds / Q000737:chemistry; D014182:Transplantation, Autologous