title: A single tyrosine hydroxyl group almost entirely controls the NADPH specificity of Plasmodium falciparum ferredoxin-NADP+ reductase.
abstract: Plasmodium falciparum ferredoxin-NADP(+) reductase (FNR) is a FAD-containing enzyme that, in addition to be a promising target of novel antimalarial drugs, represents an excellent model of plant-type FNRs. The cofactor specificity of FNRs depends on differences in both k(cat) and K(m) values for NADPH and NADH. Here, we report that deletion of the hydroxyl group of the conserved Y258 of P. falciparum FNR, which interacts with the 2'-phosphate group of NADPH, selectively decreased the k(cat) of the NADPH-dependent reaction by a factor of 2 to match that of the NADH-dependent one. Rapid-reaction kinetics, active-site titrations with NADP(+), and anaerobic photoreduction experiments indicated that this effect may be the consequence of destabilization of the catalytically competent conformation of bound NADPH. Moreover, because the Y258F replacement increased the K(m) for NADPH 4-fold and decreased that for NADH 3-fold, it led to a drop in the ability of the enzyme to discriminate between the coenzymes from 70- to just 1.5-fold. The impact of the Y258F change was not affected by the presence of the H286Q mutation, which is known to enhance the catalytic activity of the enzyme. Our data highlight the major role played by the Y258 hydroxyl group in determining the coenzyme specificity of P. falciparum FNR. From the general standpoint of engineering the kinetic properties of plant-type FNRs, although P. falciparum FNR is less strictly NADPH-dependent than its homologues, the almost complete abolishment of coenzyme selectivity reported here has never been accomplished before through a single mutation.
authors.affiliation: Department of Biomolecular Sciences and Biotechnology, Università degli Studi di Milano, via Celoria 26, 20133 Milano, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Baroni
authors.forename: Vittorio
authors.initials: V
authors.lastname: Pandini
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Vanoni
authors.forename: Alessandro
authors.initials: A
authors.lastname: Aliverti
mesh_terms: D019943:Amino Acid Substitution; D001665:Binding Sites; D003067:Coenzymes / Q000378:metabolism; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism*; D010963:Plasmodium falciparum / Q000201:enzymology; D013379:Substrate Specificity; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D003067:Coenzymes; D009243:NAD; D014443:Tyrosine; D009249:NADP; D005287:Ferredoxin-NADP Reductase