title: System approaches reveal the molecular networks involved in neural stem cell differentiation.
abstract: The self-renewal and multipotent potentials in neural stem cells (NSCs) maintain the normal physiological functions of central nervous system (CNS). The abnormal differentiation of NSCs would lead to CNS disorders. However, the mechanisms of how NSCs differentiate into astrocytes, oligodendrocytes (OLs) and neurons are still unclear, which is mainly due to the complexity of differentiation processes and the limitation of the cell separation method. In this study, we modeled the dynamics of neural cell interactions in a systemic approach by mining the high-throughput genomic and proteomic data, and identified 8615 genes that are involved in various biological processes and functions with significant changes during the differentiation processes. A total of 1559 genes are specifically expressed in neural cells, in which 242 genes are NSC specific, 215 are astrocyte specific, 551 are OL specific, and 563 are neuron specific. In addition, we proposed 57 transcriptional regulators specifically expressed in NSCs may play essential roles in the development courses. These findings provide more comprehensive analysis for better understanding the endogenous mechanisms of NSC fate determination.
authors.affiliation: Laboratory of Molecular Neurobiology, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.forename: Jiao
authors.initials: J
authors.lastname: Wang
authors.forename: Yuqiong
authors.initials: Y
authors.lastname: Xie
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Huang
authors.initials: H
authors.lastname: Yan
authors.forename: Zengrong
authors.initials: Z
authors.lastname: Liu
authors.forename: Tieqiao
authors.initials: T
authors.lastname: Wen
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D025941:Protein Interaction Mapping