title: A reverse genetics approach to study feline infectious peritonitis.
abstract: Feline infectious peritonitis (FIP) is a lethal immunopathological disease caused by feline coronaviruses (FCoVs). Here, we describe a reverse genetics approach to study FIP by assessing the pathogenicity of recombinant type I and type II and chimeric type I/type II FCoVs. All recombinant FCoVs established productive infection in cats, and recombinant type II FCoV (strain 79-1146) induced FIP. Virus sequence analyses from FIP-diseased cats revealed that the 3c gene stop codon of strain 79-1146 has changed to restore a full-length open reading frame (ORF).
authors.affiliation: Institut für Virologie, Justus-Liebig-Universität Giessen, Giessen, Germany.
authors.forename: Gergely
authors.initials: G
authors.lastname: Tekes
authors.forename: Danica
authors.initials: D
authors.lastname: Spies
authors.forename: Barbara
authors.initials: B
authors.lastname: Bank-Wolf
authors.forename: Volker
authors.initials: V
authors.lastname: Thiel
authors.forename: Heinz-Jürgen
authors.initials: HJ
authors.lastname: Thiel
mesh_terms: D000818:Animals; D002415:Cats; D016765:Coronavirus, Feline / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D016766:Feline Infectious Peritonitis / Q000821:virology*; D059386:Reverse Genetics / Q000379:methods*; D014774:Virulence