title: E-selectin mediates Porphyromonas gingivalis adherence to human endothelial cells.
abstract: Porphyromonas gingivalis, a major periodontal pathogen, may contribute to atherogenesis and other inflammatory cardiovascular diseases. However, little is known about interactions between P. gingivalis and endothelial cells. E-selectin is a membrane protein on endothelial cells that initiates recruitment of leukocytes to inflamed tissue, and it may also play a role in pathogen attachment. In the present study, we examined the role of E-selectin in P. gingivalis adherence to endothelial cells. Human umbilical vein endothelial cells (HUVECs) were stimulated with tumor necrosis factor alpha (TNF-α) to induce E-selectin expression. Adherence of P. gingivalis to HUVECs was measured by fluorescence microscopy. TNF-α increased adherence of wild-type P. gingivalis to HUVECs. Antibodies to E-selectin and sialyl Lewis X suppressed P. gingivalis adherence to stimulated HUVECs. P. gingivalis mutants lacking OmpA-like proteins Pgm6 and -7 had reduced adherence to stimulated HUVECs, but fimbria-deficient mutants were not affected. E-selectin-mediated P. gingivalis adherence activated endothelial exocytosis. These results suggest that the interaction between host E-selectin and pathogen Pgm6/7 mediates P. gingivalis adherence to endothelial cells and may trigger vascular inflammation.
authors.affiliation: Department of Oral Disease Research, National Center for Geriatrics and Gerontology at Obu, Aichi, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Komatsu
authors.forename: Keiji
authors.initials: K
authors.lastname: Nagano
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Sugiura
authors.forename: Makoto
authors.initials: M
authors.lastname: Hagiwara
authors.forename: Naomi
authors.initials: N
authors.lastname: Tanigawa
authors.forename: Yuki
authors.initials: Y
authors.lastname: Abiko
authors.forename: Fuminobu
authors.initials: F
authors.lastname: Yoshimura
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Furuichi
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsushita
mesh_terms: D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D019040:E-Selectin / Q000378:metabolism*; D042783:Endothelial Cells / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D008856:Microscopy, Fluorescence; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D019040:E-Selectin; C496834:Pgm6 protein, Porphyromonas gingivalis; C496835:Pgm7 protein, Porphyromonas gingivalis; C546936:SELE protein, human; D014409:Tumor Necrosis Factor-alpha