title: Study on the interaction between novel spiro pyrrolidine and bovine serum albumin by spectroscopic techniques.
abstract: Spiro pyrrolidines, which were proved with diverse and potent biological activities and they were discovered widespread in nature. In this paper, using fluorescence and ultraviolet spectroscopy, we investigated the interactions between novel spiro pyrrolidine (NSP) and bovine serum albumin (BSA) under the imitated physiological condition. The results show that the NSP binds to BSA molecules. Static quenching and non-radiation energy transfer are the main reasons for fluorescence quenching. We calculated the binding constant (K(a)) and binding sites (n) at different temperatures and obtained the binding distance between the tryptophan residue in BSA and the NSP based on the Förster theory of non-radiation energy transfer. In addition, using synchronous fluorescence spectra, we demonstrated conformation changes of BSA caused by NSP. The comparison of binding potency of NSP and BSA suggests that the substituent on the benzene ring influences the binding ability of NSP and BSA.
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Xin
authors.initials: X
authors.lastname: Zou
authors.forename: Hongwen
authors.initials: H
authors.lastname: Tao
authors.forename: Yulin
authors.initials: Y
authors.lastname: Ling
authors.forename: Qing
authors.initials: Q
authors.lastname: Yao
authors.forename: Hu
authors.initials: H
authors.lastname: Zhou
authors.forename: Pinggui
authors.initials: P
authors.lastname: Yi
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004735:Energy Transfer; D007700:Kinetics; D008968:Molecular Conformation; D011485:Protein Binding; D011759:Pyrrolidines / Q000737:chemistry / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013141:Spiro Compounds / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
chemical_list: D011759:Pyrrolidines; D013141:Spiro Compounds; D012710:Serum Albumin, Bovine