title: Characterization of the peridinin-chlorophyll a-protein complex in the dinoflagellate Symbiodinium.
abstract: The water-soluble peridinin-chlorophyll a-proteins (PCPs) are one of the major light harvesting complexes in photosynthetic dinoflagellates. PCP contains the carotenoid peridinin as its primary pigment. In this study, we identified and characterized the PCP protein and the PCP gene organization in Symbiodinium sp. CS-156. The protein molecular mass is 32.7kDa, revealing that the PCP is of the monomeric form. The intronless PCP genes are organized in tandem arrays. The PCP gene cassette is composed of 1095-bp coding regions and spacers in between. Despite the heterogeneity of PCP gene tandem repeats, we identified a single form of PCP, the sequence of which exactly matches the deduced sequence of PCP gene clone 7 (JQ395030) by LC-MS/MS analysis of tryptic digested PCP, revealing the mature PCP apoprotein is 312 amino acids in length. Pigment analysis showed a peridinin-to-Chl a ratio of 4. The peridinin-to-Chl a Q(y) energy transfer efficiency is 95% in this complex.
authors.affiliation: Department of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Yisheng
authors.initials: Y
authors.lastname: Kang
authors.forename: David
authors.initials: D
authors.lastname: Bina
authors.forename: Cynthia S
authors.initials: CS
authors.lastname: Lo
authors.forename: Robert E
authors.initials: RE
authors.lastname: Blankenship
mesh_terms: D000042:Absorption; D000595:Amino Acid Sequence; D002338:Carotenoids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D061146:Chlorophyll Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002853:Chromatography, Liquid; D004141:Dinoflagellida / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D013058:Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D061146:Chlorophyll Binding Proteins; D046912:Multiprotein Complexes; C016040:peridinin; D002338:Carotenoids; D000432:Methanol