title: [Preparation of chiral monolithic column with covalently bonded cellulose and their application to rapid enantioseparation].
abstract: A chiral monolithic capillary column for rapid enantioseparation was prepared by covalently bonding of cellulose tris(4-methylbenzoate) (CTMB) on N-acryloxysuccinimide-based monolith. The preparation and derivatization conditions of the monolithic column were optimized. The successful grafting of CTMB was confirmed on the characterizations of the infrared spectrum and the cathodic electroosmotic flow (EOF). The effects of acetic acid concentration and methanol content on the enantioseparation were studied. The solvent resistance, reproducibility and stability of the monolithic column have also been investigated. The rapid enantioseparation of the five solutes (phenylalanine, tyrosine, tryptophan, propranolol and phenylethanol) with resolution (R(s)) values up to 1.31 was achieved within 1.2 min on the prepared chiral capillary monolithic column by capillary electrochromatography.
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors.forename: Jiabin
authors.initials: J
authors.lastname: Wang
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Haixia
authors.initials: H
authors.lastname: Lü
authors.forename: Xucong
authors.initials: X
authors.lastname: Lin
authors.forename: Zenghong
authors.initials: Z
authors.lastname: Xie
authors.forename: Qiqing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D001565:Benzoates / Q000138:chemical synthesis / Q000737:chemistry*; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D002482:Cellulose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry; D011433:Propranolol / Q000737:chemistry; D013237:Stereoisomerism; D013388:Succinimides / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D001565:Benzoates; D013388:Succinimides; C090722:tris(4-methylbenzoate)cellulose; D014443:Tyrosine; D010649:Phenylalanine; D002482:Cellulose; D011433:Propranolol