title: Crystallization and crystallographic analysis of the ligand-binding domain of the Pseudomonas putida chemoreceptor McpS in complex with malate and succinate.
abstract: Methyl-accepting chemotaxis proteins (MCPs) are transmembrane proteins that sense changes in environmental signals, generating a chemotactic response and regulating other cellular processes. MCPs are composed of two main domains: a ligand-binding domain (LBD) and a cytosolic signalling domain (CSD). Here, the crystallization of the LBD of the chemoreceptor McpS (McpS-LBD) is reported. McpS-LBD is responsible for sensing most of the TCA-cycle intermediates in the soil bacterium Pseudomonas putida KT2440. McpS-LBD was expressed, purified and crystallized in complex with two of its natural ligands (malate and succinate). Crystals were obtained by both the counter-diffusion and the hanging-drop vapour-diffusion techniques after pre-incubation of McpS-LBD with the ligands. The crystals were isomorphous and belonged to space group C2, with two molecules per asymmetric unit. Diffraction data were collected at the ESRF synchrotron X-ray source to resolutions of 1.8 and 1.9 Å for the malate and succinate complexes, respectively.
authors.affiliation: Laboratorio de Estudios Crystalográficos, Instituto Andaluz de Ciencias de la Tierra (Consejo Superior de Investigaciones Cientificas-Universidad de Granada), Avenida De Las Palmeras 4, E-18100 Granada, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Gavira
authors.forename: J
authors.initials: J
authors.lastname: Lacal
authors.forename: J L
authors.initials: JL
authors.lastname: Ramos
authors.forename: J M
authors.initials: JM
authors.lastname: García-Ruiz
authors.forename: T
authors.initials: T
authors.lastname: Krell
authors.forename: E
authors.initials: E
authors.lastname: Pineda-Molina
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D008024:Ligands; D008293:Malates / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D016958:Pseudomonas putida / Q000737:chemistry* / Q000378:metabolism; D019802:Succinic Acid / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008024:Ligands; D008293:Malates; C030298:malic acid; D019802:Succinic Acid