title: Exposure to probiotic Lactobacillus acidophilus L-92 modulates gene expression profiles of epithelial Caco-2 cells.
abstract: To understand host gastrointestinal response after exposure to probiotic Lactobacillus acidophilus L-92, microarray analysis of cultured epithelial Caco-2 cells was performed. Of the 187 genes down-regulated after 4 h treatment with L-92, 25 were involved in RNA splicing; 12, in cell cycle; 8 were transcriptional regulators; 2 were involved in ubiquitin proteolysis; 2, in adhesion; 2, in meiosis; 2, in splicing; and 2 encoding cytokines. In the RNA splicing group, genes encoding small nuclear RNAs, nuclear pore complex interacting proteins, RNA binding motif proteins, and SMG1 homologs (phosphatidylinositol 3-kinase-related kinase) were identified. Among the only 13 genes up-regulated by the treatment, 5 were involved in histone structure, and 2 were involved in metabolism. Genes belonging to cell adhesion, transmembrane proteins, mitogen-activated protein kinase, immune response, DNA binding, inflammation, and protein synthesis groups were mainly up-regulated after 20 h of treatment, whereas no significantly down-regulated genes were observed. In the present transcriptome analysis, during the early stage of treatment (four hours of treatment) with L-92, genes involved in cell growth and cell meiosis were mainly repressed. During the late phase of treatment (20 h of treatment), the expression of the genes linked to cell adhesion activity and metabolism for cell growth was enhanced. From the present transcriptome analysis, we suggest that Caco-2 cells slow down cell death and turnover of RNA synthesis as an early response to L-92 treatment; at the late stage of treatment, the genes involved in cell proliferation, transcriptional activity, and apoptosis are activated.
authors.affiliation: Microbiology and Fermentation Laboratory, Calpis Co., Ltd., Sagamihara, Kanagawa, Japan.
authors.forename: Sae
authors.initials: S
authors.lastname: Yanagihara
authors.forename: Shinji
authors.initials: S
authors.lastname: Fukuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohno
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D017209:Apoptosis / Q000235:genetics; D018938:Caco-2 Cells; D016923:Cell Death / Q000235:genetics*; D049109:Cell Proliferation; D041981:Gastrointestinal Tract / Q000502:physiology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007779:Lactobacillus acidophilus; D046228:Microarray Analysis; D019936:Probiotics; D012313:RNA / Q000096:biosynthesis*; D014158:Transcription, Genetic; D059467:Transcriptome
chemical_list: D012313:RNA