title: Crystallization of a novel metal-containing cupin from Acidobacterium sp. and preliminary diffraction data analysis.
abstract: Recombinant AciX9_0562 from Acidobacterium sp. MP5ACTX9 (UniProt ID E8WYN5) containing sequence motifs characteristic of the RmlC-type cupins superfamily and containing Pfam motif PF07883 has been successfully cloned, expressed and purified. AciX9_0562 crystallized in a number of conditions from the Morpheus protein crystallization screen. The best crystal diffracted to 2.7 Å resolution (space group C222(1); unit-cell parameters a = 125.29, b = 254.63, c = 82.99 Å). Structure solution was facilitated by the automated molecular-replacement pipeline BALBES. The initial solution was automatically rebuilt using the PHENIX AutoBuild wizard, with final R and R(free) values of 0.23 and 0.26, respectively. The structure is currently undergoing manual refinement.
authors.affiliation: ACIB - Austrian Centre of Industrial Biotechnology GmbH, Petersgasse 14, 8010 Graz, Austria. andrzej.lyskowski@acib.at
authors.forename: Andrzej
authors.initials: A
authors.lastname: Łyskowski
authors.forename: Kerstin
authors.initials: K
authors.lastname: Steiner
authors.forename: Ivan
authors.initials: I
authors.lastname: Hajnal
authors.forename: Georg
authors.initials: G
authors.lastname: Steinkellner
authors.forename: Helmut
authors.initials: H
authors.lastname: Schwab
authors.forename: Karl
authors.initials: K
authors.lastname: Gruber
mesh_terms: D061271:Acidobacteria / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray