title: Glycogen synthase kinase 3β (GSK3β) modulates antiviral activity of zinc-finger antiviral protein (ZAP).
abstract: Zinc-finger antiviral protein (ZAP) is a host factor that specifically inhibits the replication of certain viruses, including HIV-1, Ebola virus, and Sindbis virus. ZAP binds directly to specific viral mRNAs and recruits cellular mRNA degradation machinery to degrade the target RNA. ZAP has also been suggested to repress translation of the target mRNA. In this study, we report that ZAP is phosphorylated by glycogen synthase kinase 3β (GSK3β). GSK3β sequentially phosphorylated Ser-270, Ser-266, Ser-262, and Ser-257 of rat ZAP. Inhibition of GSK3β by inhibitor SB216763 or down-regulation of GSK3β by RNAi reduced the antiviral activity of ZAP. These results indicate that phosphorylation of ZAP by GSK3β modulates ZAP activity.
authors.affiliation: Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Lv
authors.forename: Xuemin
authors.initials: X
authors.lastname: Guo
authors.forename: Guangxia
authors.initials: G
authors.lastname: Gao
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D015723:Gene Library; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D057809:HEK293 Cells; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D016086:Lentivirus / Q000235:genetics*; D016180:Lentivirus Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008301:Maleimides / Q000494:pharmacology; D010766:Phosphorylation / Q000502:physiology; D034741:RNA, Small Interfering / Q000235:genetics; D012367:RNA, Viral / Q000378:metabolism; D016601:RNA-Binding Proteins; D051381:Rats; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D007211:Indoles; D008301:Maleimides; D034741:RNA, Small Interfering; D012367:RNA, Viral; D016601:RNA-Binding Proteins; C417521:SB 216763; C465959:Zc3hav1 protein, rat; D013912:Threonine; D012694:Serine; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D038362:Glycogen Synthase Kinase 3