title: Hydroxyapatite coating for titanium fibre mesh scaffold enhances osteoblast activity and bone tissue formation.
abstract: This study investigated the bone regeneration properties of titanium fibre mesh as a tissue engineering material. A thin hydroxyapatite (HA) coating on the titanium fibre web was created using the developed molecular precursor method without losing the complex interior structure. HA-coated titanium fibre mesh showed apatite crystal formation in vitro in a human osteoblast culture. Titanium fibre mesh discs with or without a thin HA coating were implanted into rat cranial bone defects, and the animals were killed at 2 and 4 weeks. The in vivo experience revealed that the amount of newly formed bone was significantly higher in the HA-coated titanium fibre mesh than in the non-coated titanium fibre mesh 2 weeks after implantation. These results suggest that thin HA coating enhances osteoblast activity and bone regeneration in the titanium fibre mesh scaffold. Thin HA-coating improved the ability of titanium fibre mesh to act as a bone regeneration scaffold.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan. mhirota@med.yokohama-cu.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirota
authors.forename: Tohru
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshinari
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ametani
authors.forename: Takaki
authors.initials: T
authors.lastname: Shima
authors.forename: Yuka
authors.initials: Y
authors.lastname: Monden
authors.forename: Tomomichi
authors.initials: T
authors.lastname: Ozawa
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Sato
authors.forename: Chika
authors.initials: C
authors.lastname: Koyama
authors.forename: Naoto
authors.initials: N
authors.lastname: Tamai
authors.forename: Toshinori
authors.initials: T
authors.lastname: Iwai
authors.forename: Iwai
authors.initials: I
authors.lastname: Tohnai
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002118:Calcium / Q000032:analysis; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D004285:Dogs; D017886:Durapatite; D004577:Electron Probe Microanalysis; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D010758:Phosphorus / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000601:surgery; D013499:Surface Properties; D013526:Surgical Mesh; D054457:Tissue Scaffolds; D014025:Titanium
chemical_list: D020099:Coated Materials, Biocompatible; D010758:Phosphorus; D017886:Durapatite; D014025:Titanium; D002118:Calcium