title: The use of autologous enriched bone marrow MSCs to enhance osteoporotic bone defect repair in long-term estrogen deficient goats.
abstract: Bone defects are common in elderly patients suffering from osteoporosis. Current methods of bone defect treatment for osteoporosis are not always satisfactory. In this study, we demonstrated that bone marrow mesenchymal stem cells (MSCs) harvested from goats with long-term estrogen deficiencies exhibited a lower proliferation rate and decreased osteogenic capacity, which are critical obstacles for bone defect repair in the elderly. However, by combining autologous enriched bone marrow mesenchymal stem cells with porous β-TCP, we successfully repaired critical-sized bone defects in the medial femoral condyle of the osteoporotic goats. Both micro-CT images and histomorphometry analysis illustrated improved bone formation following the enriched MSC therapy. Thus, we proposed autologous enriched bone marrow mesenchymal stem cells as a quick, safe therapeutic strategy to treat osteoporotic bone defects.
authors.affiliation: Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.forename: Guangwang
authors.initials: G
authors.lastname: Liu
authors.forename: Yaokai
authors.initials: Y
authors.lastname: Gan
authors.forename: Qiming
authors.initials: Q
authors.lastname: Fan
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zhang
authors.forename: Tingting
authors.initials: T
authors.lastname: Tang
authors.forename: Kerong
authors.initials: K
authors.lastname: Dai
mesh_terms: D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D017931:DNA Primers; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D006041:Goats; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010024:Osteoporosis / Q000628:therapy*; D033581:Stem Cell Transplantation; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D017931:DNA Primers; D004967:Estrogens