title: Electrochemical aptamer-based sandwich assays for the detection of explosives.
abstract: Electrochemical impedance spectroscopy (EIS) is used to detect 2,4,6-trinitrotoluene (TNT) in a novel sandwiched structure which relies on the specific interactions between (i) primary amine with TNT and (ii) TNT and anti-TNT aptamer. With pure targets, the assay has a sensitivity of 10(-14) M, a dynamic range of 10(-14)-10(-3) M, and employs a small sample volume (25 μL). The method's sensitivity is comparable to state of the art optical methods with the added advantages of electrochemical detection, which can be easily miniaturized and implemented into a hand-held device.
authors.forename: Man Yi
authors.initials: MY
authors.lastname: Ho
authors.forename: Natasha
authors.initials: N
authors.lastname: D'Souza
authors.forename: Piero
authors.initials: P
authors.lastname: Migliorato
mesh_terms: D000838:Anions / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D055664:Electrochemical Techniques; D004566:Electrodes; D053834:Explosive Agents / Q000032:analysis*; D014303:Trinitrotoluene / Q000032:analysis
chemical_list: D000838:Anions; D052157:Aptamers, Nucleotide; D053834:Explosive Agents; D014303:Trinitrotoluene