title: [Effects of electroacupuncture on embryo implanted potential for patients with infertility of different symptom complex].
abstract: OBJECTIVE
  To explore the impacts of electroacupuncture on embryo implanted potential and its molecular mechanism in the patients with infertility of different symptom complex.
  
  
  METHODS
  Among the patients with infertility treated with electroacupuncture and in vitro fertilization and embryo transplantation (IVF-ET), 82 cases of kidney deficiency (group A), 74 cases of liver qi stagnation (group B) and 54 cases of phlegm dampness (group C) were selected. All of the patients in three groups received long-program ovarian hyper-stimulation. Additionally, electroacupuncturecan was applied before controlled ovarian hyper-stimulation (COH) and in the process of ovarian hyper-stimulation. The levels of human leukocyte antigen-G (HLA-G) in the serum were determined on the 2nd day of the menstruation (M2), on the day of human Chorionic Gonadotropin (hCG) injection and on the day of embryo transplantation in the culture solution in three groups separately. The fertilization rate, implantation rate and clinical pregnancy rate were observed for the patients in three groups.
  
  
  RESULTS
  The levels of HLA-G in the serum on hCG injection day and in the culture solution on embryo transplantation day in group A and B were significantly higher than those in group C (all P < 0.05). But, there was no significant difference in serum HLA-G levels on M2 day among three groups. The high-quality embryo rate in either group A (73.6%, 352/478) or group B (70.6%, 379/537) was higher significantly than that in group C (54.2%, 208/384) separately, presenting statistical significant difference (all P < 0.01). But there were no significant differences in clinical pregnancy rate, fertilization rate and cleavage rate among three groups.
  
  
  CONCLUSIONS
  Electroacupuncture can increase the contents of HLA-G in the body and the level of HLA-G secreted in embryos for the patients in the process of IVF-ET. Eventually, the pregnancy outcome and the pregnancy rate are improved. The clinical effects of electroacupuncture for the patients of kidney deficiency and liver qi stagnation are better than those for the patients of phlegm dampness.
authors.affiliation: Department of Reproduction, The Second Affiliated Hospital of Shandong University of TCM, Jinan 250001, China. glwxyh@163.com
authors.forename: Feng-Yun
authors.initials: FY
authors.lastname: Kong
authors.forename: Qi-Yao
authors.initials: QY
authors.lastname: Zhang
authors.forename: Qun
authors.initials: Q
authors.lastname: Guan
authors.forename: Fu-Qin
authors.initials: FQ
authors.lastname: Jian
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
mesh_terms: D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D015671:Electroacupuncture; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D059951:HLA-G Antigens / Q000097:blood; D006801:Humans; D007246:Infertility / Q000097:blood / Q000188:drug therapy / Q000503:physiopathology / Q000628:therapy*; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate
chemical_list: D006063:Chorionic Gonadotropin; D059951:HLA-G Antigens