title: Effect of a novel phytase on growth performance, apparent metabolizable energy, and the availability of minerals and amino acids in a low-phosphorus corn-soybean meal diet for broilers.
abstract: The addition of microbial phytase to diets for broiler chickens has been shown to improve the availability of phytate P, total P, some other minerals, and amino acids. In this study, the effect of a novel microbial phytase expressed by synthetic genes in Aspergillus oryzae on amino acid and mineral availability was assessed. Phytase was incorporated (1,000 and 2,000 U/kg) into low-P corn-soybean meal-based diets for broilers. Broilers received the experimental diets for 3 wk, and excreta were collected from d 18 to 21 for the determination of AME and mineral retention. On the 22nd day, the broilers were killed and the left leg removed and ileal digesta collected. Ileal phytate P and total P absorption, ileal amino acid digestibility, as well as the bone mineral content and bone mineral density were determined. Ileal phytate P absorption and absorbed phytate P content of the low-P corn-soybean meal diet were significantly (P < 0.05) higher after dietary inclusion of the novel phytase (49-60% and 65-77% higher, respectively). Apparent ileal total P absorption and apparent total P retention was 12 to 16% and 14 to 19% higher (P < 0.05), respectively, after dietary inclusion of phytase. The bone mineral content and bone mineral density in the tibia were 32 to 35% and 19 to 21% higher (P < 0.05), respectively, after dietary phytase inclusion. The apparent ileal digestibility of threonine, tyrosine, and histidine increased significantly (P < 0.05) by 14, 9, and 7%, respectively, after dietary inclusion of microbial phytase. Overall, the inclusion of a novel microbial phytase into a low-P corn-soybean meal diet for broiler chickens greatly increased phytate P and total P absorption, bone mineral content and density, as well as the digestibility of some amino acids.
authors.affiliation: Riddet Institute, Massey University, Palmerston North, New Zealand. S.M.Rutherford@massey.ac.nz
authors.forename: S M
authors.initials: SM
authors.lastname: Rutherfurd
authors.forename: T K
authors.initials: TK
authors.lastname: Chung
authors.forename: D V
authors.initials: DV
authors.lastname: Thomas
authors.forename: M L
authors.initials: ML
authors.lastname: Zou
authors.forename: P J
authors.initials: PJ
authors.lastname: Moughan
mesh_terms: D010832:6-Phytase / Q000494:pharmacology*; D000596:Amino Acids / Q000493:pharmacokinetics*; D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001682:Biological Availability; D015519:Bone Density; D002645:Chickens / Q000254:growth & development*; D004032:Diet / Q000662:veterinary; D004044:Dietary Proteins / Q000378:metabolism; D004063:Digestion; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects*; D008903:Minerals / Q000493:pharmacokinetics*; D016226:Phosphorus, Dietary / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
chemical_list: D000596:Amino Acids; D004044:Dietary Proteins; D008903:Minerals; D016226:Phosphorus, Dietary; D010832:6-Phytase