title: Safety and efficacy of antioxidants-loaded nanoparticles for an anti-aging application.
abstract: The aim of this work was to perform a pilot study on the safety and efficacy of nanoparticle formulation for cosmetic application. The encapsulated actives in the nanoparticles were a blend of coenzyme Q10, retinyl palmitate, tocopheryl acetate, grape seed oil and linseed oil. The nanoparticle suspension was characterized in terms of pH and particle size. For the safety assessment, alternative methods as cytotoxicity and HET CAM were used. The clinical skin compatibility tests were also performed. The efficacy was evaluated in healthy volunteers presenting different degrees of periorbital wrinkles. Skin hydration was performed by corneometry. The nanoparticles presented narrow size around 140 nm and pH close to neutral and were suitable to cutaneous application. The alternative tests demonstrated that the nanoparticles did not present potential to induce skin irritant effects, cytotoxicity or generate oxidative stress. The clinical assays confirmed the in vitro results, demonstrating the safety of the nanoparticles, which were not irritant, sensitizing and comedogenic. Furthermore, the exposure to UVA light did not cause photoxicity. Regarding the efficacy, nanoparticles presented significant reduction in wrinkle degree after 21 days of application compared to the control. The volunteers could differentiate the nanoparticles and the control product by means of subjective analyses. In conclusion, the nanoparticles containing antioxidant actives were safe for topical use and presented anti-aging activity in vivo and are suitable to be used as cosmetic ingredient.
authors.affiliation: Inventiva Indústria e Inovação ltda, Rua Piraju, 255, Porto Alegre, RS, Brazil, CEP 91030-190.
authors.forename: Cândice C
authors.initials: CC
authors.lastname: Felippi
authors.forename: Dileusa
authors.initials: D
authors.lastname: Oliveira
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ströher
authors.forename: Anderson R
authors.initials: AR
authors.lastname: Carvalho
authors.forename: Eliana A M Aquino
authors.initials: EA
authors.lastname: Van Etten
authors.forename: Márcia
authors.initials: M
authors.lastname: Bruschi
authors.forename: Renata P
authors.initials: RP
authors.lastname: Raffin
mesh_terms: D000328:Adult; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000009:adverse effects* / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D002839:Chromans / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry; D003358:Cosmetics / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry; D004224:Diterpenes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008043:Linseed Oil / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000008:administration & dosage* / Q000009:adverse effects* / Q000737:chemistry; D010316:Particle Size; D000084562:Retinyl Esters; D012867:Skin / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects*; D023422:Skin Irritancy Tests; D013473:Sunscreening Agents / Q000009:adverse effects / Q000737:chemistry; D024505:Tocopherols / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry; D014451:Ubiquinone / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives / Q000737:chemistry; D014801:Vitamin A / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002839:Chromans; D003358:Cosmetics; D004224:Diterpenes; D000084562:Retinyl Esters; D013473:Sunscreening Agents; D014801:Vitamin A; D014451:Ubiquinone; C014794:retinol palmitate; D008043:Linseed Oil; C024989:coenzyme Q10; D024505:Tocopherols; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid