title: Low concentrations of bisphenol A induce lipid accumulation mediated by the production of reactive oxygen species in the mitochondria of HepG2 cells.
abstract: Bisphenol A (BPA) is an endocrine-disrupting chemical that leaches from polycarbonate plastics that consequently leads to low-dose human exposure. In addition to its known xenoendocrine action, BPA exerts a wide variety of metabolic effects, but no data are available on its actions on the functions of liver mitochondrial. To assess these effects, HepG2 cells were exposed to BPA (10(-4)-10(-12)M) and physiological parameters were measured by flow cytometry. We demonstrated a significant mitochondrial dysfunction including ROS production, ΔΨ(M) hyperpolarization, lipid accumulation, lipoperoxidation and the release of pro-inflammatory cytokines. In conclusion, we showed that low concentrations of BPA promote lipid accumulation in hepatic cells triggered by disturbances in mitochondrial function, alterations in lipid metabolism and by inflammation that can therefore contribute to steatosis.
authors.affiliation: INRA, UMR1331, Toxalim, Research Centre in Food Toxicology, F-31027 Toulouse, France. Laurence.Huc@toulouse.inra.fr
authors.forename: Laurence
authors.initials: L
authors.lastname: Huc
authors.forename: Anthony
authors.initials: A
authors.lastname: Lemarié
authors.forename: Françoise
authors.initials: F
authors.lastname: Guéraud
authors.forename: Cécile
authors.initials: C
authors.lastname: Héliès-Toussaint
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors / Q000633:toxicity*; D056945:Hep G2 Cells; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D010636:Phenols / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D000447:Aldehydes; D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D016209:Interleukin-8; D009569:Nitric Oxide; D010636:Phenols; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C027576:4-hydroxy-2-nonenal; C006780:bisphenol A; D000098826:Bisphenol A Compounds