title: Expression analysis of four peroxiredoxin genes from Tamarix hispida in response to different abiotic stresses and Exogenous Abscisic Acid (ABA).
abstract: Peroxiredoxins (Prxs) are a recently discovered family of antioxidant enzymes that catalyze the reduction of peroxides and alkyl peroxides. In this study, four Prx genes (named as ThPrxII, ThPrxIIE, ThPrxIIF, and Th2CysPrx) were cloned from Tamarix hispida. Their expression profiles in response to stimulus of NaCl, NaHCO(3), PEG, CdCl(2) and abscisic acid (ABA) in roots, stems and leaves of T. hispida were investigated using real-time RT-PCR. The results showed that the four ThPrxs were all expressed in roots, stems and leaves. Furthermore, the transcript levels of ThPrxIIE and ThPrxII were the lowest and the highest, respectively, in all tissue types. All the ThPrx genes were induced by both NaCl and NaHCO(3) and reached their highest expression levels at the onset of stress in roots. Under PEG and CdCl(2) stress, the expression patterns of these ThPrxs showed temporal and spatial specificity. The expressions of the ThPrxs were all differentially regulated by ABA, indicating that they are all involved in the ABA signaling pathway. These findings reveal a complex regulation of Prxs that is dependent on the type of Prx, tissue, and the signaling molecule. The divergence of the stress-dependent transcriptional regulation of the ThPrx gene family in T. hispida may provide an essential basis for the elucidation of Prx function in future work.
authors.affiliation: State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, China.
authors.forename: Caiqiu
authors.initials: C
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, China.
authors.forename: Kaimin
authors.initials: K
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, China.
authors.forename: Guiyan
authors.initials: G
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, 150040 Harbin, China.
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000975:Antioxidants; D019256:Cadmium Chloride / Q000494:pharmacology; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D054464:Peroxiredoxins / Q000096:biosynthesis* / Q000235:genetics*; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D021382:Protein Sorting Signals / Q000235:genetics; D016415:Sequence Alignment; D017693:Sodium Bicarbonate / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D032363:Tamaricaceae / Q000187:drug effects* / Q000235:genetics
chemical_list: D000975:Antioxidants; D021382:Protein Sorting Signals; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D000040:Abscisic Acid; D017693:Sodium Bicarbonate; D054464:Peroxiredoxins; D019256:Cadmium Chloride