title: Hydrothermal synthesis of GSH-TGA co-capped CdTe quantum dots and their application in labeling colorectal cancer cells.
abstract: We have successfully synthesized GSH and TGA co-capped CdTe quantum dots (QDs) with good biological compatibility and high fluorescence intensity. The effects of different reaction time, temperature, pH value, ligand concentration and the molar ratio of GSH/TGA were carefully investigated to optimize the synthesis condition. The optical properties of as-prepared CdTe QDs were studied by UV-visible absorption spectrum and fluorescence spectrum, meanwhile their structure and morphology were characterized using transmission electron microscope (TEM), Fourier transform infrared spectra (FT-IR) and X-ray powder diffraction (XRD). Compared with the CdTe QDs that are single-capped with either GSH or TGA, the GSH-TGA co-capped CdTe QDs demonstrated significantly improved fluorescence intensity and optical stability. In addition, GSH-TGA co-capped CdTe QDs were conjugated to amonoclonal antibody ND-1. The GSH-TGA co-capped CdTe QDs-antibody probe was successfully used to label colorectal cancer cells, CCL187, in vitro.
authors.affiliation: Department of Chemistry, Northeastern University, Shenyang 110819, PR China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yu
authors.forename: Linru
authors.initials: L
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Huanyu
authors.initials: H
authors.lastname: Gao
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Fang
authors.forename: Shukun
authors.initials: S
authors.lastname: Xu
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D015179:Colorectal Neoplasms / Q000473:pathology*; D005978:Glutathione / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D057054:Molecular Imaging; D045663:Quantum Dots; D013691:Tellurium / Q000737:chemistry*; D013864:Thioglycolates / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D013864:Thioglycolates; C017487:2-mercaptoacetate; D005978:Glutathione; D013691:Tellurium; C028337:cadmium telluride