title: Prognostic significance of miR-205 in endometrial cancer.
abstract: OBJECTIVE
  microRNAs have emerged as key regulators of gene expression, and their altered expression has been associated with tumorigenesis and tumor progression. Thus, microRNAs have potential as both cancer biomarkers and/or potential novel therapeutic targets. Although accumulating evidence suggests the role of aberrant microRNA expression in endometrial carcinogenesis, there are still limited data available about the prognostic significance of microRNAs in endometrial cancer. The goal of this study is to investigate the prognostic value of selected key microRNAs in endometrial cancer by the analysis of archival formalin-fixed paraffin-embedded tissues.
  
  
  METHODS
  Total RNAs were extracted from 48 paired normal and endometrial tumor specimens using Trizol based approach. The expression of miR-26a, let-7g, miR-21, miR-181b, miR-200c, miR-192, miR-215, miR-200c, and miR-205 were quantified by real time qRT-PCR expression analysis. Targets of the differentially expressed miRNAs were quantified using immunohistochemistry. Statistical analysis was performed by GraphPad Prism 5.0.
  
  
  RESULTS
  The expression levels of miR-200c (P<0.0001) and miR-205 (P<0.0001) were significantly increased in endometrial tumors compared to normal tissues. Kaplan-Meier survival analysis revealed that high levels of miR-205 expression were associated with poor patient overall survival (hazard ratio, 0.377; Logrank test, P = 0.028). Furthermore, decreased expression of a miR-205 target PTEN was detected in endometrial cancer tissues compared to normal tissues.
  
  
  CONCLUSIONS
  miR-205 holds a unique potential as a prognostic biomarker in endometrial cancer.
authors.affiliation: Department of Pathology, Stony Brook University, Stony Brook, New York, United States of America.
authors.forename: Mihriban
authors.initials: M
authors.lastname: Karaayvaz
authors.forename: Cecilia
authors.initials: C
authors.lastname: Zhang
authors.forename: Sharon
authors.initials: S
authors.lastname: Liang
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Shroyer
authors.forename: Jingfang
authors.initials: J
authors.lastname: Ju
mesh_terms: D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D035683:MicroRNAs / Q000235:genetics*; D011379:Prognosis
chemical_list: D035683:MicroRNAs; C533896:MIRN200 microRNA, human; C534425:MIR205, human; C524940:MIRN21 microRNA, human; C553667:MIRN215 microRNA, human; C528163:MIRN26 microRNA, human; C540193:MIrn181 microRNA, human