title: Serological antibodies against LY6K as a diagnostic biomarker in esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  To evaluate the diagnostic values of autoantibodies against lymphocyte antigen 6 complex locus K (LY6K) in esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  After cloning, expressing, and purifying LY6K as fusion proteins, LY6K autoantibodies were measured in 62 patient and 58 control serum samples using enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the LY6K mRNA levels in ESCC and adjacent tissues.
  
  
  RESULTS
  LY6K autoantibodies were found significantly higher in patients than controls. The area under the receiver-operating characteristic (ROC) curve (AUC) was 0.85, and the optimal sensitivity and specificity for ESCC detection were 80.6 and 78.7%, respectively. LY6K mRNA expressions in patients were upregulated.
  
  
  CONCLUSIONS
  Autoantibodies against LY6K may be a good diagnostic biomarker for ESCC.
authors.affiliation: Department of Cell Biology, Hebei University College of Life Sciences, Baoding, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Zhengliang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xufei
authors.initials: X
authors.lastname: Zhang
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
authors.forename: Kemp H
authors.initials: KH
authors.lastname: Kernstine
authors.forename: Li
authors.initials: L
authors.lastname: Zhong
mesh_terms: D000328:Adult; D000368:Aged; D000912:Antibodies, Neoplasm / Q000097:blood*; D000950:Antigens, Ly / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D016022:Case-Control Studies; D003001:Cloning, Molecular; D004938:Esophageal Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D000912:Antibodies, Neoplasm; D000950:Antigens, Ly; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D058851:GPI-Linked Proteins; C525725:LY6K protein, human