title: Lack of evidence from studies of soluble protein fragments that Knops blood group polymorphisms in complement receptor-type 1 are driven by malaria.
abstract: Complement receptor-type 1 (CR1, CD35) is the immune-adherence receptor, a complement regulator, and an erythroid receptor for Plasmodium falciparum during merozoite invasion and subsequent rosette formation involving parasitized and non-infected erythrocytes. The non-uniform geographical distribution of Knops blood group CR1 alleles Sl1/2 and McC(a/b) may result from selective pressures exerted by differential exposure to infectious hazards. Here, four variant short recombinant versions of CR1 were produced and analyzed, focusing on complement control protein modules (CCPs) 15-25 of its ectodomain. These eleven modules encompass a region (CCPs 15-17) key to rosetting, opsonin recognition and complement regulation, as well as the Knops blood group polymorphisms in CCPs 24-25. All four CR1 15-25 variants were monomeric and had similar axial ratios. Modules 21 and 22, despite their double-length inter-modular linker, did not lie side-by-side so as to stabilize a bent-back architecture that would facilitate cooperation between key functional modules and Knops blood group antigens. Indeed, the four CR1 15-25 variants had virtually indistinguishable affinities for immobilized complement fragments C3b (K(D) = 0.8-1.1 µM) and C4b (K(D) = 5.0-5.3 µM). They were all equally good co-factors for factor I-catalysed cleavage of C3b and C4b, and they bound equally within a narrow affinity range, to immobilized C1q. No differences between the variants were observed in assays for inhibition of erythrocyte invasion by P. falciparum or for rosette disruption. Neither differences in complement-regulatory functionality, nor interactions with P. falciparum proteins tested here, appear to have driven the non-uniform geographic distribution of these alleles.
authors.affiliation: The Institute of Structural and Molecular Biology, University of Edinburgh, Edinburgh, United Kingdom.
authors.forename: Patience B
authors.initials: PB
authors.lastname: Tetteh-Quarcoo
authors.forename: Christoph Q
authors.initials: CQ
authors.lastname: Schmidt
authors.forename: Wai-Hong
authors.initials: WH
authors.lastname: Tham
authors.forename: Richard
authors.initials: R
authors.lastname: Hauhart
authors.forename: Haydyn D T
authors.initials: HD
authors.lastname: Mertens
authors.forename: Arthur
authors.initials: A
authors.lastname: Rowe
authors.forename: John P
authors.initials: JP
authors.lastname: Atkinson
authors.forename: Alan F
authors.initials: AF
authors.lastname: Cowman
authors.forename: J Alexandra
authors.initials: JA
authors.lastname: Rowe
authors.forename: Paul N
authors.initials: PN
authors.lastname: Barlow
mesh_terms: D000483:Alleles; D000818:Animals; D001789:Blood Group Antigens / Q000235:genetics* / Q000378:metabolism*; D003179:Complement C3b / Q000235:genetics / Q000378:metabolism; D015935:Complement C4b / Q000235:genetics / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D008288:Malaria / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D010271:Parasites / Q000235:genetics / Q000378:metabolism; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D010963:Plasmodium falciparum / Q000235:genetics / Q000378:metabolism; D011110:Polymorphism, Genetic; D011951:Receptors, Complement / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001789:Blood Group Antigens; D011951:Receptors, Complement; D003179:Complement C3b; D015935:Complement C4b