title: Edwardsiella tarda flagellar protein FlgD: a protective immunogen against edwardsiellosis.
abstract: Edwardsiella tarda is a gram-negative bacterium and a causative agent of edwardsiellosis, resulting to severe loss of the aquaculture industry. In this study, based on the reverse vaccinology, sixteen flagellar proteins were selected from highly pathogenic E. tarda EIB202 genome information and in silico analyzed as potential vaccine candidates. Among them, ten recombinant proteins were highly expressed in Escherichia coli and successfully purified. The immunoprotective potentials of these purified recombinant proteins were evaluated in zebrafish model. And recombinant FlgD and FliD were found to lead to a high relative percent survival (RPS, about 70%) against E. tarda EIB202. Furthermore, FlgD required in flagellum hook assembly brought about the similar immune protection in turbot. The immune responses of zebrafish and turbot to recombinant FlgD were also investigated, and the results indicated that its high protection was mainly involved in cellular mediated immune response, corresponding to the intracellular pathogenicity of E. tarda.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Haizhen
authors.initials: H
authors.lastname: Wu
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Li
authors.forename: Minjun
authors.initials: M
authors.lastname: Yang
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Qiyao
authors.initials: Q
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D020609:Edwardsiella tarda / Q000235:genetics / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000517:prevention & control / Q000662:veterinary*; D004926:Escherichia coli / Q000235:genetics; D005408:Flagellin / Q000235:genetics / Q000276:immunology*; D005412:Flatfishes; D015870:Gene Expression; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D016019:Survival Analysis; D015027:Zebrafish
chemical_list: D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D005408:Flagellin