title: Antimicrobial peptide incorporated poly(2-hydroxyethyl methacrylate) hydrogels for the prevention of Staphylococcus epidermidis-associated biomaterial infections.
abstract: The effectiveness of the antimicrobial peptide maximin-4, the ultrashort peptide H-Orn-Orn-Trp-Trp-NH(2), and the lipopeptide C(12)-Orn-Orn-Trp-Trp-NH(2) in preventing adherence of pathogens to a candidate biomaterial were tested utilizing both matrix- and immersion-loaded poly(2-hydroxyethyl methacrylate) (poly(HEMA)) hydrogels. Antiadherent properties correlated to both the concentration released and the relative antimicrobial concentrations of each compound against Staphylococcus epidermidis ATCC 35984, at each time point. Immersion-loaded samples containing C(12)-Orn-Orn-Trp-Trp-NH(2) exhibited the lowest adherence profile for all peptides studied over 1, 4, and 24 h. The results outlined in this article show that antimicrobial peptides have the potential to serve as an important weapon against biomaterial associated infections.
authors.affiliation: Biomaterials Research Group, School of Pharmacy, Queens University of Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, United Kingdom.
authors.forename: Garry
authors.initials: G
authors.lastname: Laverty
authors.forename: Sean P
authors.initials: SP
authors.lastname: Gorman
authors.forename: Brendan F
authors.initials: BF
authors.lastname: Gilmore
mesh_terms: D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000737:chemistry*; D001672:Biocompatible Materials; D020100:Hydrogels; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate; D013203:Staphylococcal Infections / Q000382:microbiology / Q000517:prevention & control*; D013212:Staphylococcus epidermidis / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D020100:Hydrogels; D010455:Peptides; D011102:Polyhydroxyethyl Methacrylate