title: The yields of transcripts for a RNA polymerase regulated by hairpin structures in nascent RNAs.
abstract: The yields of transcripts capable of forming RNA hairpins with different stem lengths at elongation phase were determined in a T7 RNA polymerase. The amount of RNA transcripts decreased with stem length, demonstrating that transcriptional efficiency is regulated by secondary structure formation in the nascent RNA.
authors.affiliation: Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Kobe 650-0047, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Nagatoishi
authors.forename: Ryoya
authors.initials: R
authors.lastname: Ono
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
mesh_terms: D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D055029:Inverted Repeat Sequences; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D012313:RNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases