title: [A method for the analysis of overlapped peaks in the high performance liquid chromatogram based on spectrum analysis].
abstract: A method was established to analyse the overlapped chromatographic peaks based on the chromatographic-spectra data detected by the diode-array ultraviolet detector. In the method, the three-dimensional data were de-noised and normalized firstly; secondly the differences and clustering analysis of the spectra at different time points were calculated; then the purity of the whole chromatographic peak were analysed and the region were sought out in which the spectra of different time points were stable. The feature spectra were extracted from the spectrum-stable region as the basic foundation. The nonnegative least-square method was chosen to separate the overlapped peaks and get the flow curve which was based on the feature spectrum. The three-dimensional divided chromatographic-spectrum peak could be gained by the matrix operations of the feature spectra with the flow curve. The results displayed that this method could separate the overlapped peaks.
authors.affiliation: National Center for Quality Supervision and Inspection of Processed Food (Shandong), Shandong Supervision and Inspection Institute for Product Quality, Jinan 250100, China. 290435932@qq.com
authors.forename: Bao
authors.initials: B
authors.lastname: Liu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Fan
authors.forename: Shengnan
authors.initials: S
authors.lastname: Huo
authors.forename: Lili
authors.initials: L
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Hu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu