title: Evaluation of Biocompatibility of Alloplastic Materials: Development of a Tissue Culture In Vitro Test System.
abstract: Optimized biocompatibility is a major requirement for alloplastic materials currently applied in surgical approaches for hernia, incontinence, and prolapse situations. Tissue ingrowth/adherence and formation of connective tissue seem to have important influence in mesh incorporation at the implant site. In an in vitro approach we randomly investigated 7 different mesh types currently used in surgeries with various indications with regard to their adherence performance. Using a tissue culture approach, meshes were incubated with tissue representative of fibroblasts, muscle cells, and endothelial cells originating from 10 different patients. After 6 weeks, the meshes were assessed microscopically and a ranking of their adherence performance was established. Tissue culture was successful in 100% of the probes. We did not remark on interindividual differences concerning the growth and adherence performance after incubation with the different meshes in the investigated 10 patients. The ranking was consistent in all patients. In this test system, PVDF Dynamesh® (FEG Textiltechnik, Aachen, Germany) was the mesh with the best growth-in score. The test system was feasible and reproducible. Pore size seems to be a predictor of adherence performance. The test system may be a helpful tool for further investigations, and the predictive value should be assessed in further in vitro and in vivo experiments.
authors.affiliation: Consultant, Department of Urology, Lukas Hospital, Neuss, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Gerullis
authors.affiliation: Department of Urology, Lukas Hospital, Neuss, Germany.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Georgas
authors.affiliation: Department of Urology, Lukas Hospital, Neuss, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Eimer
authors.affiliation: Department of General Surgery, Lukas Hospital, Neuss, Germany.
authors.forename: Peter E
authors.initials: PE
authors.lastname: Goretzki
authors.affiliation: Department of General Surgery, Lukas Hospital, Neuss, Germany.
authors.forename: Bernhard J
authors.initials: BJ
authors.lastname: Lammers
authors.affiliation: German Centre for Implant-Pathology, Duren, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Klosterhalfen
authors.affiliation: Department of Experimental Surgery, University of Szeged, Szeged, Hungary.
authors.forename: Mihaly
authors.initials: M
authors.lastname: Boros
authors.affiliation: Department of Urology, Theodor Bilharz Research Institute, Cairo, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Wishahi
authors.affiliation: Institute for Pathophysiology, University of Essen, Essen, Germany.
authors.forename: Gerd
authors.initials: G
authors.lastname: Heusch
authors.affiliation: Department of Urology, Lukas Hospital, Neuss, Germany, Oncologist, West German Cancer Center, University of Essen, Essen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Otto