title: [The effect of the essential oil from Aloysia triphylla britton (lemon verbena) on Trypanosoma cruzi in mice].
abstract: OBJECTIVE
  To determine the in-vivo anti-Trypanosoma cruzi activity of the essential oil from Aloysia triphylla in mice.
  
  
  METHODS
  The mice (n = 15) in the study were randomly assigned to the following groups: infected and untreated (G1), infected and treated with benznidazole 100 mg/kg (G2), infected and treated with of Aloysia triphylla essential oil 100 mg/kg (G3), infected and treated with of Aloysia triphylla essential oil 250 mg/kg (G4), uninfected and untreated (G5), and uninfected and treated with 250 mg/kg of Aloysia triphyla (G6). The infection was conducted using 104 blood T. cruzi trypomastigotes and the treatment began on the 8th day post-infection (dpi) through 28 dpi. The parasitemia was determined through optical microscopy every 2 days using 5 μL of blood sampled from the tail. In dpi 14, 21 and 28, the blood sample was obtained from the queue for testing creatine kinase-MB, alanine aminotransferase and creatinine, then animals were euthanized and the heart was removed for histopathological study.
  
  
  RESULTS
  The essential oil of lemon verbena produced a significant reduction of the parasitemia, 85.4% with the peak at a the dose of 250 mg/ kg. It was also observed a reduction in the number of amastigotes and inflammatory infiltrates in the heart. The creatine kinase-MB plasma levels also decreased at dpi 28 as a result of such treatment.
  
  
  CONCLUSIONS
  Under experimental conditions, the essential oil of Aloysia triphylla (lemon verbena) has in vivo anti-Trypanosoma cruzi effect in mice.
authors.affiliation: Instituto de Investigaciones Clínicas, Universidad Nacional Mayor de San Marcos, Lima, Perú. jprojasarmas@yahoo.com
authors.forename: Juan
authors.initials: J
authors.lastname: Rojas
authors.forename: Olga
authors.initials: O
authors.lastname: Palacios
authors.forename: Sergio
authors.initials: S
authors.lastname: Ronceros
mesh_terms: D000818:Animals; D008297:Male; D051379:Mice; D009822:Oils, Volatile / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects*; D032412:Verbena
chemical_list: D009822:Oils, Volatile