title: Resident Corneal Cells Communicate with Neutrophils Leading to the Production of IP-10 during the Primary Inflammatory Response to HSV-1 Infection.
abstract: In this study we show that murine and human neutrophils are capable of secreting IP-10 in response to communication from the HSV-1 infected cornea and that they do so in a time frame associated with the recruitment of CD8(+) T cells and CXCR3-expressing cells. Cellular markers were used to establish that neutrophil influx corresponded in time to peak IP-10 production, and cellular depletion confirmed neutrophils to be a significant source of IP-10 during HSV-1 corneal infection in mice. A novel ex vivo model for human corneal tissue infection with HSV-1 was used to confirm that cells resident in the cornea are also capable of stimulating neutrophils to secrete IP-10. Our results support the hypothesis that neutrophils play a key role in T-cell recruitment and control of viral replication during HSV-1 corneal infection through the production of the T-cell recruiting chemokine IP-10.
authors.affiliation: Department of Biology, University of West Georgia, Carrollton, GA 30118, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Molesworth-Kenyon
authors.forename: N
authors.initials: N
authors.lastname: Popham
authors.forename: A
authors.initials: A
authors.lastname: Milam
authors.forename: J E
authors.initials: JE
authors.lastname: Oakes
authors.forename: R N
authors.initials: RN
authors.lastname: Lausch