title: MiR-206 regulates neural cells proliferation and apoptosis via Otx2.
abstract: MiR-206 was involved in a series of cellular activities, such as the growth and development of skeletal muscle and the tumorigenesis. MiR-206 was characterized previously as a differentially expressed gene in sodium arsenite (SA)-induced neural tube defects (NTDs) in chick embryos via miRNA microarray analysis. However, the role of miR-206 in the pathological process of nerve cells remained elusive. In this study we found differential expression of miR-206 in SA-treated chick embryos by Northern blot analysis. Ectopic expression of miR-206 inhibited cell proliferation, and promoted cell apoptosis in U343 and SK-N-SH cell by using MTT, Edu Apollo assay and Flow cytometry analysis. Further investigation revealed that miR-206 can interact with 3'-untranslated region (UTR) of Otx2. MiR-206 mimics down-regulated the endogeneous Otx2 expression, whereas the miR-206 inhibitor obviously up-regulated the expression of Otx2. These findings indicate that overexpression of miR-206 promotes cell apoptosis and low expression of miR-206 inhibits cell apoptosis. Otx2 may play an important role in the process of miR-206-mediated cell apoptosis.
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.forename: Ge
authors.initials: G
authors.lastname: Song
authors.forename: Chan Juan
authors.initials: CJ
authors.lastname: Hao
authors.forename: Yi
authors.initials: Y
authors.lastname: Cui
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Xia
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D017209:Apoptosis; D018053:Arsenites / Q000494:pharmacology; D001665:Binding Sites; D015152:Blotting, Northern; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D002642:Chick Embryo; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009436:Neural Tube Defects / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051857:Otx Transcription Factors / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D017670:Sodium Compounds / Q000494:pharmacology; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D018053:Arsenites; C546087:MIRN206 microRNA, human; D035683:MicroRNAs; C497659:OTX2 protein, human; D051857:Otx Transcription Factors; D017670:Sodium Compounds; C017947:sodium arsenite