title: Live free or die: stretch-induced apoptosis occurs when adaptive reorientation of annulus fibrosus cells is restricted.
abstract: High matrix strains in the intervertebral disc occur during physiological motions and are amplified around structural defects in the annulus fibrosus (AF). It remains unknown if large matrix strains in the human AF result in localized cell death. This study investigated strain amplitudes and substrate conditions where AF cells were vulnerable to stretch-induced apoptosis. Human degenerated AF cells were subjected to 1 Hz-cyclic tensile strains for 24h on uniformly collagen coated substrates and on substrates with 40 μm stripes of collagen that restricted cellular reorientation. AF cells were capable of responding to stretch (stress fibers and focal adhesions aligned perpendicular to the direction of stretch), but were vulnerable to stretch-induced apoptosis when cytoskeletal reorientation was restricted, as could occur in degenerated states due to fibrosis and crosslink accumulation and at areas where high strains occur (around structural defects, delaminations, and herniations).
authors.affiliation: School of Engineering, University of Vermont, Burlington, VT 05405, USA. Rosalyn.Abbott@uvm.edu
authors.forename: Rosalyn D
authors.initials: RD
authors.lastname: Abbott
authors.forename: Alan K
authors.initials: AK
authors.lastname: Howe
authors.forename: Helene M
authors.initials: HM
authors.lastname: Langevin
authors.forename: James C
authors.initials: JC
authors.lastname: Iatridis
mesh_terms: D017209:Apoptosis; D002478:Cells, Cultured; D022001:Focal Adhesions / Q000648:ultrastructure; D006801:Humans; D007403:Intervertebral Disc / Q000166:cytology* / Q000502:physiology; D022502:Stress Fibers / Q000648:ultrastructure; D013314:Stress, Mechanical; D013312:Stress, Physiological; D013718:Tensile Strength