title: Evaluation of a synthetic C34 trimer of HIV-1 gp41 as AIDS vaccines.
abstract: An artificial antigen forming the C34 trimeric structure targeting membrane-fusion mechanism of HIV-1 has been evaluated as an HIV vaccine. The C34 trimeric molecule was previously designed and synthesized using a novel template with C3-symmetric linkers by us. The antiserum produced by immunization of the C34 trimeric form antigen showed 23-fold higher binding affinity for the C34 trimer than for the C34 monomer and showed significant neutralizing activity. The present results suggest effective strategies of the design of HIV vaccines and anti-HIV agents based on the native structure mimic of proteins targeting dynamic supramolecular mechanisms in HIV fusion.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Hashimoto
authors.forename: Wataru
authors.initials: W
authors.lastname: Nomura
authors.forename: Aki
authors.initials: A
authors.lastname: Ohya
authors.forename: Emiko
authors.initials: E
authors.lastname: Urano
authors.forename: Kosuke
authors.initials: K
authors.lastname: Miyauchi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Narumi
authors.forename: Haruo
authors.initials: H
authors.lastname: Aikawa
authors.forename: Jun A
authors.initials: JA
authors.lastname: Komano
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tamamura
mesh_terms: D016915:AIDS Vaccines / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D019380:Anti-HIV Agents / Q000737:chemistry; D002460:Cell Line; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000276:immunology*; D015488:HIV Antigens / Q000737:chemistry / Q000276:immunology; D015700:HIV Envelope Protein gp41 / Q000138:chemical synthesis / Q000276:immunology*; D006801:Humans; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D019380:Anti-HIV Agents; D015483:HIV Antibodies; D015488:HIV Antigens; D015700:HIV Envelope Protein gp41; D010446:Peptide Fragments; C407149:peptide C34