title: Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus.
abstract: Fish have diverse sex determination and differentiation. DMRT1 and aromatase are conserved in the phyla and play pivotal roles in sex development. Gobiocypris rarus is a small fish used as a model in aquatic toxicology in China and has been used to study the effects of environmental endocrine disruptors on gene expression, but its sexual development remains elusive. Here, we report the full-length cDNA of G. rarus dmrt1 and its expression along with the expression of cyp19a1a and cyp19a1b, two genes encoding gonad and brain type aromatases, in adults and during ontogenesis. Both cyp19a1a and dmrt1 are expressed in the ovary and testis but show sexual dimorphism. Expression of cyp19a1a in the ovary is higher than in testes and dmrt1 follows the opposite pattern. Juvenile gonad histology changes at 15 days after hatching. The dimorphic expression of dmrt1 and cyp19a1a appears from 5 days after hatching, which is earlier than histological change. cyp19a1b is expressed coordinately with cyp19a1a until 15 days after hatching. These results show that dmrt1 and cyp19a1a play important roles in sex determination and sex differentiation in G. rarus.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrated Biology, College of Life Sciences, Central China Normal University, Wuhan, Hubei 430079, China.
authors.forename: Mengxi
authors.initials: M
authors.lastname: Cao
authors.forename: Jundan
authors.initials: J
authors.lastname: Duan
authors.forename: Nana
authors.initials: N
authors.lastname: Cheng
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhong
authors.forename: Zequn
authors.initials: Z
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Haobin
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D017124:Conserved Sequence; D003530:Cyprinidae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005260:Female; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012727:Sex Characteristics; D012733:Sex Differentiation; D013737:Testis / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: C110547:DMRT1 protein; D029941:Fish Proteins; D014157:Transcription Factors; D001141:Aromatase