title: Suppressive subtractive hybridization libraries prepared from the digestive gland of the oyster Crassostrea brasiliana exposed to a diesel fuel water-accommodated fraction.
abstract: Diesel fuel can cause adverse effects in marine invertebrates by mechanisms that are not clearly understood. The authors used suppressive subtractive hybridization to identify genes up- and downregulated in Crassostrea brasiliana exposed to diesel fuel. Genes putatively involved in protein regulation, innate immune, and stress response, were altered by diesel challenge. Three genes regulated by diesel were validated by quantitative real-time polymerase chain reaction. This study sheds light on transcriptomic responses of oysters to diesel pollution.
authors.affiliation: Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, NEPAQ, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
authors.forename: Karim Hahn
authors.initials: KH
authors.lastname: Lüchmann
authors.forename: Jacó Joaquim
authors.initials: JJ
authors.lastname: Mattos
authors.forename: Marília Nardelli
authors.initials: MN
authors.lastname: Siebert
authors.forename: Tarquin Stephen
authors.initials: TS
authors.lastname: Dorrington
authors.forename: Guilherme
authors.initials: G
authors.lastname: Toledo-Silva
authors.forename: Patricia Hermes
authors.initials: PH
authors.lastname: Stoco
authors.forename: Edmundo Carlos
authors.initials: EC
authors.lastname: Grisard
authors.forename: Afonso Celso Dias
authors.initials: AC
authors.lastname: Bainy
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D049896:Crassostrea / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D004784:Environmental Monitoring; D005742:Gasoline / Q000633:toxicity*; D015723:Gene Library; D006824:Hybridization, Genetic; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D005742:Gasoline; D014874:Water Pollutants, Chemical