title: Cellular interactions of surface modified nanoporous silicon particles.
abstract: In this study, the self-assembly of hydrophobin class II (HFBII) on the surface of thermally hydrocarbonized porous silicon (THCPSi) nanoparticles was investigated. The HFBII-coating converted the hydrophobic particles into more hydrophilic ones, improved the particles' cell viability in both HT-29 and Caco-2 cell lines compared to uncoated particles, and enhanced the particles' cellular association. The amount of HFBII adsorbed onto the particles was also successfully quantified by both the BCA assay and a HPLC method. Importantly, the permeation of a poorly water-soluble drug, indomethacin, loaded into THCPSi particles across Caco-2 monolayers was not affected by the protein coating. In addition, (125)I-radiolabelled HFBII did not extensively permeate the Caco-2 monolayer and was found to be stably adsorbed onto the THCPSi nanoparticles incubated in pH 7.4, which renders the particles the possibility for further track-imaging applications. The results highlight the potential of HFBII coating for improving wettability, increasing biocompatibility and possible intestinal association of PSi nanoparticulates for drug delivery applications.
authors.affiliation: Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, FI-00014, Finland. luis.bimbo@helsinki.fi
authors.forename: Luis M
authors.initials: LM
authors.lastname: Bimbo
authors.forename: Mirkka
authors.initials: M
authors.lastname: Sarparanta
authors.forename: Ermei
authors.initials: E
authors.lastname: Mäkilä
authors.forename: Timo
authors.initials: T
authors.lastname: Laaksonen
authors.forename: Päivi
authors.initials: P
authors.lastname: Laaksonen
authors.forename: Jarno
authors.initials: J
authors.lastname: Salonen
authors.forename: Markus B
authors.initials: MB
authors.lastname: Linder
authors.forename: Jouni
authors.initials: J
authors.lastname: Hirvonen
authors.forename: Anu J
authors.initials: AJ
authors.lastname: Airaksinen
authors.forename: Hélder A
authors.initials: HA
authors.lastname: Santos
mesh_terms: D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D005434:Flow Cytometry; D006801:Humans; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry; D016062:Porosity; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004337:Drug Carriers; D012825:Silicon