title: Sarcoma cells induce alteration in adipogenic differentiation.
abstract: BACKGROUND
  Sarcomas consist of both tumor and stromal cells, and the interaction between these compartments is critical for tumor progression and metastasis. Tumor-derived factors may alter the differentiation capacity of the adjacent stromal cells. The aim of this study was to elucidate the paracrine impact of liposarcoma cells on pre-adipocytes, their adipogenic differentiation process and miRNA expression profile.
  
  
  METHODS
  3T3-L1 pre-adipocytes were exposed to sarcoma pre-conditioned media. Following induction of adipogenic differentiation morphometrical changes were assessed. Differences in miRNA expression of conditioned and non-conditioned 3T3-L1 cells were analyzed.
  
  
  RESULTS
  Exposure to sarcoma pre-conditioned media substantially altered the differentiation capability of 3T3-L1 pre-adipocytes. Significant changes in the miRNA expression profile between conditioned and non-conditioned pre-adipocytes were observed.
  
  
  CONCLUSIONS
  Sarcoma cells directly modulate pre-adipocyte differentiation. This study demonstrates that sarcoma cells influence differentiation of pre-adipocytes via paracrine factors and alter their miRNA expression.
authors.affiliation: Department of Plastic Surgery, Reference Centre for Soft Tissue Sarcoma, BG University Hospital Bergmannsheil, Ruhr-University Bochum, Bochum, North Rhine-Westphalia, Germany. D.Tilkorn@web.de
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Tilkorn
authors.forename: Sammy
authors.initials: S
authors.lastname: Al-Benna
authors.forename: Joerg
authors.initials: J
authors.lastname: Hauser
authors.forename: Andrej
authors.initials: A
authors.lastname: Ring
authors.forename: Hans Ulrich
authors.initials: HU
authors.lastname: Steinau
authors.forename: Andrea
authors.initials: A
authors.lastname: Tannapfel
authors.forename: Ingo
authors.initials: I
authors.lastname: Stricker
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism; D012509:Sarcoma / Q000473:pathology*
chemical_list: D035683:MicroRNAs