title: Synthesis and properties of RNAs that contain a PNA-RNA dimer.
abstract: A practical synthesis of a peptide nucleic acid unit combined with an RNA nucleoside (PNA-RNA dimer) is reported. The dimer unit was placed within an RNA oligonucleotide via phosphoramidite chemistry and melting temperature data indicate destabilization relative to a native RNA duplex. Circular dichroism indicates that the overall shape of the duplex remains intact. This PNA-RNA dimer unit will permit future investigations within RNA-based systems, such as RNA interference.
authors.affiliation: Faculty of Science, University of Ontario Institute of Technology, Oshawa, Ontario, Canada.
authors.forename: Wei
authors.initials: W
authors.lastname: Gong
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Desaulniers
mesh_terms: D060326:Chemistry Techniques, Synthetic / Q000379:methods*; D019281:Dimerization; D009691:Nucleic Acid Denaturation; D009943:Organophosphorus Compounds / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry*; D044366:Transition Temperature
chemical_list: D009943:Organophosphorus Compounds; D020135:Peptide Nucleic Acids; C434331:phosphoramidite; D012313:RNA