title: Cell processing for haplo-identical hematopoietic stem cell transplantation: automated washing and immunomagnetic-positive selection.
abstract: OBJECTIVE
  Immunomagnetic cell selection (ICS) of CD34(+) cells is being used increasingly in allogeneic transplantation in order to reduce T-cell quantity. The aim of this study was to evaluate an automated washing protocol before immunomagnetic selection.
  
  
  METHODS
  The automated method was compared with a conventional washing procedure. In the study group the cell processing using the automated procedure, both before and after antibody incubation, was performed with a Sepax S-100 device. The efficacy of the automated procedure was compared with the control group, where washing were performed using a standard method.
  
  
  RESULTS
  The results obtained after pre-incubation washing performed using the automated system showed a total nucleated cell (NC) and CD34(+) cell recovery of 84.87% (71.80-105, SD 8.62; range, standard deviation) and 83.45% (47-109, SD 16.12), respectively. The NC and CD34(+) cell recovery after the pre-incubation washing cycle was performed using the standard method was 75.54% (38.36-97.76, SD 22.5) and 61.51% (30.87-81.79, SD 19.3), respectively. The CD34(+) cell recovery after ICS was 51.27% (13.77-98.82, SD 24.97) and 48.89% (15.57-88.24, SD 25.91) for group 1 and group 2, respectively. The average purity in group 1 was 86.46% (67.4-96.10, SD 13.07) and in group 2 84.97% (58.1-97.8, SD 15.58).
  
  
  CONCLUSIONS
  The efficacy of the ICS led to an optimal purity without affecting cell recovery, which was higher in group 1. Overall, our data suggest that the automated method is suitable for washing hematopoietic progenitor cell apheresis (HPC-A) concentrates before immunomagnetic cell selection in daily clinical routines.
authors.affiliation: Immunohematology Section, Tor Vergata University, CryoLab and Stem Cells Manipulation and Cryopreservation Laboratory, IRCCS Bambino Gesù Pediatric Hospital, Rome, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Scerpa
authors.forename: Nicola
authors.initials: N
authors.lastname: Daniele
authors.forename: Chiara
authors.initials: C
authors.lastname: Ciammetti
authors.forename: Cecilia
authors.initials: C
authors.lastname: Rossi
authors.forename: Pietro
authors.initials: P
authors.lastname: Sodani
authors.forename: Alessandro
authors.initials: A
authors.lastname: Lanti
authors.forename: Guido
authors.initials: G
authors.lastname: Lucarelli
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Isacchi
authors.forename: Francesco
authors.initials: F
authors.lastname: Zinno
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000276:immunology*; D001781:Blood Component Removal; D018929:Cell Culture Techniques; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation / Q000379:methods; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D018952:Antigens, CD34