title: [Effect of early dermatoplastic repair on testicular Survivin protein expression in juvenile pigs with entire third degree burn wound of scrotum].
abstract: OBJECTIVE
  To explore the effect of early scrotal dermatoplasty on spermatogenic functional rehabilitation of testis in juvenile pigs with third degree burn wound of the scrotum.
  
  
  METHODS
  Thirty healthy male Guizhou miniature pigs (weighing 10-15 kg, 2-month-old) were divided into 3 groups: control group (group A, n = 10), natural healing group (group B, n = 10), and dermatoplasty group (group C, n = 10). In group A, the pig was not given any treatment; after third degree burn model of the scrotum was prepared, wounds were not treated in group B and the burn skin was excised and whole hypogastric pachydermia was used for dermatoplasty in group C. At 3 months and 1 year after model preparation, bilateral testis were collected from 5 pigs, respectively. HE staining was performed to observe the effects of different repair method on the morphology of spermatogenic cells and immunohistochemical staining was used to detect Survivin protein expression.
  
  
  RESULTS
  All pigs survived to the end of the experiment and the wound healed successfully. Histological observation showed that spermatogenic cells had normal shape at all stages and mature sperms were seen in lumens in group A; the thickness of seminiferous epithelium was thinner, having one layer or two layers of spermatogenic cells in group B; the spermatogenic cells in group C were slightly more than that in group B with some spermatids; and in groups B and C, the spermatogenic cells at 1 year were more than that at 3 months. Immunohistochemistry staining showed that the Survivin protein expression in groups B and C was less than in group A, and group B was less than group C, showing significant differences at 3 months and 1 year (P < 0.05), but no significant difference between 3 months and 1 year in the same group (P > 0.05).
  
  
  CONCLUSIONS
  Dermatoplasty has inhibitory effect on spermatogenic functional rehabilitation of testis. Dermatoplasty can decrease spermatogenic cells and reduce Survivin protein expression, but some spermatids still survive in seminiferous tubule.
authors.affiliation: Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi Guizhou 563000, PR China.
authors.forename: Xiujun
authors.initials: X
authors.lastname: Tang
authors.forename: Zairong
authors.initials: Z
authors.lastname: Wei
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Sun
authors.forename: Chengliang
authors.initials: C
authors.lastname: Deng
authors.forename: Dali
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000601:surgery*; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D008297:Male; D012611:Scrotum / Q000293:injuries; D016038:Skin Transplantation / Q000379:methods; D013552:Swine; D013556:Swine, Miniature; D013737:Testis / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D051034:Inhibitor of Apoptosis Proteins