title: Atrazine and PCB 153 and their effects on the proteome of subcellular fractions of human MCF-7 cells.
abstract: Several man-made organic pollutants including polychlorinated biphenyls (PCBs) and several pesticides may exhibit endocrine disrupting (ED) properties. These ED molecules can be comparatively persistent in the environment, and have shown to perturb hormonal activity and several physiological functions. The objective of this investigation was to study the impact of PCB 153 and atrazine on human MCF-7 cells, and to search for marker proteins of their exposure. Cells were exposed to environmentally high but relevant concentrations of atrazine (200ppb), PCB 153 (500ppb), 17-β estradiol (positive control, 10nM) and DMSO (0.1%, negative control) for t=36h (n=3 replicates/exposure group). Proteins from cell membrane and cytosol were isolated, and studied by 2D-DiGE. Differentially regulated proteins were trypsin-digested and identified by MALDI-ToF-ToF and NCBInr database. A total of 36 differentially regulated proteins (>|1.5| fold change, P<0.05) were identified in the membrane fraction and 22 in the cytosol, and were mainly involved in cell structure and in stress response, but also in xenobiotic metabolism. 67% (membrane) and 50% (cytosol) of differentially regulated proteins were more abundant following atrazine exposure whereas nearly 100% (membrane) and 45% (cytosol) were less abundant following PCB 153 exposure. Western blots of selected proteins (HSBP1, FKBP4, STMN1) confirmed 2D-DiGE results. This study emphasizes the numerous potential effects that ED compounds could have on exposed humans.
authors.affiliation: Department of Environment and Agro-Biotechnologies, Centre de Recherche Public - Gabriel Lippmann, Belvaux, Luxembourg.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Lasserre
authors.forename: Fred
authors.initials: F
authors.lastname: Fack
authors.forename: Tommaso
authors.initials: T
authors.lastname: Serchi
authors.forename: Dominique
authors.initials: D
authors.lastname: Revets
authors.forename: Sébastien
authors.initials: S
authors.lastname: Planchon
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
authors.forename: Lucien
authors.initials: L
authors.lastname: Hoffmann
authors.forename: Arno C
authors.initials: AC
authors.lastname: Gutleb
authors.forename: Claude P
authors.initials: CP
authors.lastname: Muller
authors.forename: Torsten
authors.initials: T
authors.lastname: Bohn
mesh_terms: D001280:Atrazine / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology / Q000502:physiology; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins; D006801:Humans; D061986:MCF-7 Cells; D008565:Membrane Proteins / Q000378:metabolism; D018832:Molecular Chaperones; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D020543:Proteome / Q000378:metabolism*; D050777:Stathmin / Q000378:metabolism; D022021:Tacrolimus Binding Proteins / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D020543:Proteome; C493995:STMN1 protein, human; D050777:Stathmin; D004958:Estradiol; D011078:Polychlorinated Biphenyls; D022021:Tacrolimus Binding Proteins; C403485:tacrolimus binding protein 4; D001280:Atrazine; C014024:2,4,5,2',4',5'-hexachlorobiphenyl