title: D-serine-dehydratase from Saccaromyces cerevisiae: a pyridoxal 5'- phosphate-dependent enzyme for advanced biotech applications.
abstract: The Saccaromices cerevisiae D-serine dehydratase is a pyridoxal 5'-phosphate dependent enzyme that requires zinc for its function. It catalyses the conversion of D-serine into pyruvate and ammonia with the K(m) and k(cat) values of 0.39 mM and 13.1 s(-1) respectively. In this work, a new methodology for monitoring D-serine is presented. Our results show that this enzyme could be successfully used as a biological probe for detection of D-serine via fluorescence spectroscopy.
authors.affiliation: Laboratory for Molecular Sensing, IBP-CNR Via Pietro Castellino, 111 801131 Naples, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Staiano
authors.forename: Maria
authors.initials: M
authors.lastname: Strianese
authors.forename: Antonio
authors.initials: A
authors.lastname: Varriale
authors.forename: Stefano
authors.initials: S
authors.lastname: Di Giovanni
authors.forename: Donatella Scotto
authors.initials: DS
authors.lastname: di Mase
authors.forename: Valentina
authors.initials: V
authors.lastname: Dell'Angelo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ruggiero
authors.forename: Tullio
authors.initials: T
authors.lastname: Labella
authors.forename: Claudio
authors.initials: C
authors.lastname: Pellecchia
authors.forename: Sabato
authors.initials: S
authors.lastname: D'Auria
mesh_terms: D000042:Absorption; D015374:Biosensing Techniques / Q000379:methods*; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006836:Hydro-Lyases / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D011732:Pyridoxal Phosphate / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D012694:Serine / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D029701:Saccharomyces cerevisiae Proteins; D012694:Serine; D011732:Pyridoxal Phosphate; D006836:Hydro-Lyases; C101950:D-serine dehydratase; D019793:Fluorescein