title: Cascade signal amplification strategy for the detection of cancer cells by rolling circle amplification and nanoparticles tagging.
abstract: A cascade signal amplification strategy was proposed for detection of cancer cells at ultralow concentration by combining the rolling circle amplification (RCA) technique with oligonucleotide functionalized nanoparticles (NPs), and anodic stripping voltammetric detection. This flexible biosensing system exhibited high sensitivity and specificity with the detection limits of 10 Ramos cells mL(-1).
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, China. dingcaifeng2003@163.com
authors.forename: Caifeng
authors.initials: C
authors.lastname: Ding
authors.forename: Haitao
authors.initials: H
authors.lastname: Liu
authors.forename: Nannan
authors.initials: N
authors.lastname: Wang
authors.forename: Zhenfeng
authors.initials: Z
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D045744:Cell Line, Tumor; D015342:DNA Probes / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D009841:Oligonucleotides