title: Isothermal microcalorimetry: a novel method for real-time determination of antifungal susceptibility of Aspergillus species.
abstract: We evaluated microcalorimetry for real-time susceptibility testing of Aspergillus spp. based on growth-related heat production. The minimal heat inhibitory concentration (MHIC) for A. fumigatus ATCC 204305 was 1 mg/L for amphotericin B, 0.25 mg/L for voriconazole, 0.06 mg/L for posaconazole, 0.125 mg/L for caspofungin and 0.03 mg/L for anidulafungin. Agreement within two 2-fold dilutions between MHIC (determined by microcalorimetry) and MIC or MEC (determined by CLSI M38A) was 90% for amphotericin B, 100% for voriconazole, 90% for posaconazole and 70% for caspofungin. This proof-of-concept study demonstrated the potential of isothermal microcalorimetry for growth evaluation of Aspergillus spp. and real-time antifungal susceptibility testing.
authors.affiliation: Infectious Diseases Service, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland.
authors.forename: U
authors.initials: U
authors.lastname: Furustrand Tafin
authors.forename: M
authors.initials: M
authors.lastname: Clauss
authors.forename: P M
authors.initials: PM
authors.lastname: Hauser
authors.forename: J
authors.initials: J
authors.lastname: Bille
authors.forename: J F
authors.initials: JF
authors.lastname: Meis
authors.forename: A
authors.initials: A
authors.lastname: Trampuz
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000254:growth & development; D002151:Calorimetry / Q000379:methods*; D008826:Microbial Sensitivity Tests / Q000379:methods
chemical_list: D000935:Antifungal Agents