title: 2D-DIGE analysis of mango (Mangifera indica L.) fruit reveals major proteomic changes associated with ripening.
abstract: A comparative proteomic investigation between the pre-climacteric and climacteric mango fruits (cv. Keitt) was performed to identify protein species with variable abundance during ripening. Proteins were phenol-extracted from fruits, cyanine-dye-labeled, and separated on 2D gels at pH 4-7. Total spot count of about 373 proteins spots was detected in each gel and forty-seven were consistently different between pre-climacteric and climacteric fruits and were subjected to LC-MS/MS analysis. Functional classification revealed that protein species involved in carbon fixation and hormone biosynthesis decreased during ripening, whereas those related to catabolism and the stress-response, including oxidative stress and abiotic and pathogen defense factors, accumulated. In relation to fruit quality, protein species putatively involved in color development and pulp softening were also identified. This study on mango proteomics provides an overview of the biological processes that occur during ripening.
authors.affiliation: University of São Paulo, Department of Food Science and Experimental Nutrition, FCF, São Paulo, Brazil.
authors.forename: Jonathan de Magalhães
authors.initials: Jde M
authors.lastname: Andrade
authors.forename: Tatiana Torres
authors.initials: TT
authors.lastname: Toledo
authors.forename: Silvia Beserra
authors.initials: SB
authors.lastname: Nogueira
authors.forename: Beatriz Rosana
authors.initials: BR
authors.lastname: Cordenunsi
authors.forename: Franco Maria
authors.initials: FM
authors.lastname: Lajolo
authors.forename: João Roberto Oliveira
authors.initials: JR
authors.lastname: do Nascimento
mesh_terms: D005638:Fruit / Q000378:metabolism*; D031022:Mangifera / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D040901:Proteomics
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins