title: Searching for cellular partners of hantaviral nonstructural protein NSs: Y2H screening of mouse cDNA library and analysis of cellular interactome.
abstract: Hantaviruses (Bunyaviridae) are negative-strand RNA viruses with a tripartite genome. The small (S) segment encodes the nucleocapsid protein and, in some hantaviruses, also the nonstructural protein (NSs). The aim of this study was to find potential cellular partners for the hantaviral NSs protein. Toward this aim, yeast two-hybrid (Y2H) screening of mouse cDNA library was performed followed by a search for potential NSs protein counterparts via analyzing a cellular interactome. The resulting interaction network was shown to form logical, clustered structures. Furthermore, several potential binding partners for the NSs protein, for instance ACBD3, were identified and, to prove the principle, interaction between NSs and ACBD3 proteins was demonstrated biochemically.
authors.affiliation: Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland. samu.ronnberg@helsinki.fi
authors.forename: Tuomas
authors.initials: T
authors.lastname: Rönnberg
authors.forename: Kirsi
authors.initials: K
authors.lastname: Jääskeläinen
authors.forename: Guillaume
authors.initials: G
authors.lastname: Blot
authors.forename: Ville
authors.initials: V
authors.lastname: Parviainen
authors.forename: Antti
authors.initials: A
authors.lastname: Vaheri
authors.forename: Risto
authors.initials: R
authors.lastname: Renkonen
authors.forename: Michele
authors.initials: M
authors.lastname: Bouloy
authors.forename: Alexander
authors.initials: A
authors.lastname: Plyusnin
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D015723:Gene Library; D006476:Orthohantavirus / Q000235:genetics* / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping / Q000379:methods; D020798:Two-Hybrid System Techniques; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: C486730:Acbd3 protein, mouse; D048868:Adaptor Proteins, Signal Transducing; D008565:Membrane Proteins; D017361:Viral Nonstructural Proteins