title: Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography.
abstract: To date there is a lack of tools to map the spatio-temporal dynamics of diverse cells in experimental heart models. Conventional histology is labor intensive with limited coverage, whereas many imaging techniques do not have sufficiently high enough spatial resolution to map cell distributions. We have designed and built a high resolution, dual channel Born-normalized near-infrared fluorescence optical projection tomography system to quantitatively and spatially resolve molecular agents distribution within whole murine heart. We validated the use of the system in a mouse model of monocytes/macrophages recruitment during myocardial infarction. While acquired, data were processed and reconstructed in real time. Tomographic analysis and visualization of the key inflammatory components were obtained via a mathematical formalism based on left ventricular modeling. We observed extensive monocyte recruitment within and around the infarcted areas and discovered that monocytes were also extensively recruited into non-ischemic myocardium, beyond that of injured tissue, such as the septum.
authors.affiliation: Center for System Biology, Massachusetts General Hospital and Harvard Medical School, Richard B. Simches Research Center, Boston, Massachusetts, United States of America.
authors.forename: Claudio
authors.initials: C
authors.lastname: Vinegoni
authors.forename: Paolo
authors.initials: P
authors.lastname: Fumene Feruglio
authors.forename: Daniel
authors.initials: D
authors.lastname: Razansky
authors.forename: Rostic
authors.initials: R
authors.lastname: Gorbatov
authors.forename: Vasilis
authors.initials: V
authors.lastname: Ntziachristos
authors.forename: Andrea
authors.initials: A
authors.lastname: Sbarbati
authors.forename: Matthias
authors.initials: M
authors.lastname: Nahrendorf
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001692:Biological Transport; D002401:Cathepsin B / Q000378:metabolism; D003911:Dextrans / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D051379:Mice; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism*; D009203:Myocardial Infarction / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry; D013997:Time Factors; D041622:Tomography, Optical / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D003911:Dextrans; D015335:Molecular Probes; D002401:Cathepsin B