title: Single-wall carbon nanotubes induce oxidative stress in rat aortic endothelial cells.
abstract: Oxidative stress is a major factor contributing to endothelial cell damage. Single-wall carbon nanotubes (SWCNTs) have oxidative properties; however, the oxidative effects of SWCNTs on endothelial cells are not fully understood. In the present study, we investigated the effects of oxidative stress induced by SWCNTs on rat aortic endothelial cells (RAECs). Various markers of cellular damage were assessed, such as biochemical and ES immunity indexes, and DNA and protein damage. Our findings suggest that RAEC endured oxidative damage following SWCNT exposure. Specifically, after SWCNTs exposure, non-enzymatic antioxidant glutathione was activated prior to superoxide dismutase activation in order to defend against oxidative stress. Additionally, it was found that as SWCNT concentration increased, so did the stress protein, heme oxygenase-1 (HO-1), expression levels. These changes may induce RAEC damage, and result in many serious diseases.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin, China.
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Cheng
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Lin
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ceng
authors.forename: Bo-Fei
authors.initials: BF
authors.lastname: Wei
authors.forename: Xian-Jun
authors.initials: XJ
authors.lastname: Fan
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Hong-Lian
authors.initials: HL
authors.lastname: Yang
authors.forename: Huan-Liang
authors.initials: HL
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Lei
authors.initials: L
authors.lastname: Tian
authors.forename: Ben-Cheng
authors.initials: BC
authors.lastname: Lin
authors.forename: Shu-Mao
authors.initials: SM
authors.lastname: Ding
authors.forename: Zhu-Ge
authors.initials: ZG
authors.lastname: Xi
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology*; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D037742:Nanotubes, Carbon / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D018272:Porins; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D037742:Nanotubes, Carbon; D018272:Porins; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D016159:Tumor Suppressor Protein p53; C080060:protein IIIa, Rhizobium leguminosarum; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase