title: Molecular cloning and characterization of estrogen receptor gene in the scallop Chlamys farreri: expression profiles in response to endocrine disrupting chemicals.
abstract: In order to gain insights into the mechanism of sex steroid signaling in molluscs, the full-length cDNA of estrogen receptor (ER) was isolated and characterized from Chlamys farreri for the first time. The positions of cysteine residues and other residues around them that constitute the two zinc finger motifs and the P-box are conserved. Phylogenetic analysis revealed that the CfER is an ortholog of the other mollusk ERs. Tissue distribution analysis of the CfER mRNA revealed that the expression of ER mRNA was observed in various tissues, and highest in the gonad of males and females. C. farreri were exposed for 10 days to endocrine disrupting chemicals including Benzo(a)pyrene (B(a)p) and polybrominated diphenyl ethers (BDE-47). B(a)p exposure at 0.4 and 2 μg/L caused significant increase in mRNA expression of ER and VTG, but B(a)p at 10 μg/L down-regulated CfER and VTG mRNA expression compared to control. Varying increase of ER and VTG mRNA transcripts was resulted in by BDE-47 at 0.1, 1 and 10 μg/L. These results elucidate potential roles of CfER induced by xenobiotics in C. farreri and can be helpful for investigating the mechanism of sex steroid signaling in bivalve mollusks.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001564:Benzo(a)pyrene / Q000494:pharmacology; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D052244:Endocrine Disruptors / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling / Q000379:methods; D055768:Halogenated Diphenyl Ethers; D008297:Male; D008969:Molecular Sequence Data; D049897:Pectinidae / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011075:Polybrominated Biphenyls / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment / Q000379:methods; D014018:Tissue Distribution; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D052244:Endocrine Disruptors; D055768:Halogenated Diphenyl Ethers; D011075:Polybrominated Biphenyls; D012333:RNA, Messenger; D011960:Receptors, Estrogen; C511295:2,2',4,4'-tetrabromodiphenyl ether; D001564:Benzo(a)pyrene