title: Clinical implication of 14-3-3 epsilon expression in gastric cancer.
abstract: OBJECTIVE
  To evaluate for the first time the protein and mRNA expression of 14-3-3ε in gastric carcinogenesis.
  
  
  METHODS
  14-3-3ε protein expression was determined by western blotting, and mRNA expression was examined by real-time quantitative RT-PCR in gastric tumors and their matched non-neoplastic gastric tissue samples.
  
  
  RESULTS
  Authors observed a significant reduction of 14-3-3ε protein expression in gastric cancer (GC) samples compared to their matched non-neoplastic tissue. Reduced levels of 14-3-3ε were also associated with diffuse-type GC and early-onset of this pathology. Our data suggest that reduced 14-3-3ε may have a role in gastric carcinogenesis process.
  
  
  CONCLUSIONS
  Our results reveal that the reduced 14-3-3ε expression in GC and investigation of 14-3-3ε interaction partners may help to elucidate the carcinogenesis process.
authors.affiliation: Genetics Division, Department of Morphology and Genetic, Federal University of São Paulo, São Paulo, Brazil. mariana.morf@epm.br
authors.forename: Mariana Ferreira
authors.initials: MF
authors.lastname: Leal
authors.forename: Danielle Queiroz
authors.initials: DQ
authors.lastname: Calcagno
authors.forename: Sâmia
authors.initials: S
authors.lastname: Demachki
authors.forename: Paulo Pimentel
authors.initials: PP
authors.lastname: Assumpção
authors.forename: Roger
authors.initials: R
authors.lastname: Chammas
authors.forename: Rommel Rodríguez
authors.initials: RR
authors.lastname: Burbano
authors.forename: Marília de Arruda Cardoso
authors.initials: Mde A
authors.lastname: Smith
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology*
chemical_list: D048948:14-3-3 Proteins