title: Promotion of hair growth by Rosmarinus officinalis leaf extract.
abstract: Topical administration of Rosmarinus officinalis leaf extract (RO-ext, 2 mg/day/mouse) improved hair regrowth in C57BL/6NCrSlc mice that experienced hair regrowth interruption induced by testosterone treatment. In addition, RO-ext promoted hair growth in C3H/He mice that had their dorsal areas shaved. To investigate the antiandrogenic activity mechanism of RO-ext, we focused on inhibition of testosterone 5α-reductase, which is well recognized as one of the most effective strategies for the treatment of androgenic alopecia. RO-ext showed inhibitory activity of 82.4% and 94.6% at 200 and 500 µg/mL, respectively. As an active constituent of 5α-reductase inhibition, 12-methoxycarnosic acid was identified with activity-guided fractionation. In addition, the extract of R. officinalis and 12-methoxycarnosic acid inhibited androgen-dependent proliferation of LNCaP cells as 64.5% and 66.7% at 5 µg/mL and 5 μM, respectively. These results suggest that they inhibit the binding of dihydrotestosterone to androgen receptors. Consequently, RO-ext is a promising crude drug for hair growth.
authors.affiliation: Faculty of Pharmacy, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Murata
authors.forename: Kazuma
authors.initials: K
authors.lastname: Noguchi
authors.forename: Masato
authors.initials: M
authors.lastname: Kondo
authors.forename: Mariko
authors.initials: M
authors.lastname: Onishi
authors.forename: Naoko
authors.initials: N
authors.lastname: Watanabe
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Okamura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D058891:5-alpha Reductase Inhibitors / Q000494:pharmacology*; D000505:Alopecia / Q000139:chemically induced / Q000188:drug therapy; D000726:Androgen Antagonists / Q000494:pharmacology; D000818:Animals; D045744:Cell Line, Tumor; D006197:Hair / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D027542:Rosmarinus / Q000737:chemistry*; D013739:Testosterone / Q000494:pharmacology
chemical_list: D058891:5-alpha Reductase Inhibitors; D000726:Androgen Antagonists; D010936:Plant Extracts; D013739:Testosterone