title: Rapid on-site detection of Acidovorax citrulli by cross-priming amplification.
abstract: Cross-priming amplification (CPA) for Acidovorax citrulli detection was evaluated in this study. The sensitivity of CPA assay for pure bacterial culture was 3.7 × 10(3) CFU/ml. Bacteria on naturally infected watermelon seeds were detected using CPA assay, suggesting this method is suitable for A. citrulli on-site detection from watermelon seeds.
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Tian
authors.forename: Shui-fang
authors.initials: SF
authors.lastname: Zhu
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Zhao
authors.forename: Feng-quan
authors.initials: FQ
authors.lastname: Liu
mesh_terms: D029773:Citrullus / Q000382:microbiology; D042621:Comamonadaceae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry*; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial