title: Major histocompatibility complex class I molecules modulate embryonic neuritogenesis and neuronal polarization.
abstract: We studied cultured hippocampal neurons from embryonic wildtype, major histocompatibility complex class I (MHCI) heavy chain-deficient (K(b)D(b)-/-) and NSE-D(b) (which have elevated neuronal MHCI expression) C57BL/6 mice. K(b)D(b)-/- neurons displayed slower neuritogenesis and establishment of polarity, while NSE-D(b) neurons had faster neurite outgrowth, more primary neurites, and tended to have accelerated polarization. Additional studies with ß2M-/- neurons, exogenous ß2M, and a self-MHCI monomer suggest that free heavy chain cis interactions with other surface molecules can promote neuritogenesis while tripartite MHCI interactions with classical MHCI receptors can inhibit axon outgrowth. Together with the results of others, MHCI appears to differentially modulate neuritogenesis and synaptogenesis.
authors.affiliation: Department of Molecular and Medical Pharmacology, UCLA School of Medicine, University of California-Los Angeles, CA 90095-1735, USA.
authors.forename: Tina
authors.initials: T
authors.lastname: Bilousova
authors.forename: Hoa
authors.initials: H
authors.lastname: Dang
authors.forename: Willem
authors.initials: W
authors.lastname: Xu
authors.forename: Sarah
authors.initials: S
authors.lastname: Gustafson
authors.forename: Yingli
authors.initials: Y
authors.lastname: Jin
authors.forename: Lalinda
authors.initials: L
authors.lastname: Wickramasinghe
authors.forename: Tony
authors.initials: T
authors.lastname: Won
authors.forename: Gabriela
authors.initials: G
authors.lastname: Bobarnac
authors.forename: Blake
authors.initials: B
authors.lastname: Middleton
authors.forename: Jide
authors.initials: J
authors.lastname: Tian
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Kaufman
mesh_terms: D000199:Actins / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001369:Axons / Q000502:physiology; D016764:Cell Polarity / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000166:cytology; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000172:deficiency
chemical_list: D000199:Actins; D015395:Histocompatibility Antigens Class I; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D011994:Recombinant Proteins; D014404:Tubulin; D001613:beta 2-Microglobulin; C462941:beta3 tubulin, mouse; D010751:Phosphopyruvate Hydratase