title: In vivo Brillouin optical microscopy of the human eye.
abstract: We report the first Brillouin measurement of the human eye in vivo. We constructed a Brillouin optical scanner safe for human use by employing continuous-wave laser light at 780 nm at a low power of 0.7 mW. With a single scan along the optic axis of the eye, the axial profile of Brillouin frequency shift was obtained with a pixel acquisition time of 0.4 s and axial resolution of about 60 μm, showing the depth-dependent biomechanical properties in the cornea and lens.
authors.affiliation: Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 50 Blossom St., BHX-6, Boston, Massachusetts 02114, USA.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Scarcelli
authors.forename: Seok Hyun
authors.initials: SH
authors.lastname: Yun
mesh_terms: D000328:Adult; D003315:Cornea / Q000033:anatomy & histology / Q000502:physiology; D055119:Elastic Modulus; D004867:Equipment Design; D005123:Eye / Q000033:anatomy & histology*; D006801:Humans; D007908:Lens, Crystalline / Q000033:anatomy & histology / Q000502:physiology; D008027:Light; D008297:Male; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D009799:Ocular Physiological Phenomena; D055620:Optical Phenomena; D012542:Scattering, Radiation