title: Determination of tricyclazole content in paddy rice by surface enhanced Raman spectroscopy.
abstract: An ultrasensitive method based on Surface enhanced Raman scattering (SERS) has been developed to determine content of a pesticide which is tricyclazole in paddy rice using sliver colloid as a substrate and pyridine as an internal standard. The peaks at 424 and 1035 cm(-1) in a SERS spectrum were selected as analytic and internal peaks, respectively, and their intensity ratio I(t)/I(p) was used to calculate the regression concentration of tricyclazole. The correlation between I(t)/I(p) and concentration showed significant linear relationship with a correlation coefficient of R(2)= 0.995 in a concentration range of 0.05 to 0.70 mg/L and the tricyclazole solution can be detected to be low as 0.002 mg/L by SERS. The method was applied to determine tricyclazole contents of 3 real rice samples with a standard addition method in order to eliminate interference of matrix. The errors of SERS measurements for the 3 samples were 0.0008 to 0.0246, 0.0013 to 0.0028, and 0.0129 to 0.0304 mg/kg, respectively, compared with the results obtained by high performance liquid chromatography method. This also showed a good reproducibility with low values of relative standard deviation (n= 3) for the 3 samples ranged from 3.63% to 4.64%.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, and Research Centre of Analysis and Test, East China Univ. of Science and Technology, Meilong Rd 130, Shanghai 200237, China.
authors.forename: Huirong
authors.initials: H
authors.lastname: Tang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Fang
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Cao
authors.forename: Jinpei
authors.initials: J
authors.lastname: Geng
authors.forename: Tao
authors.initials: T
authors.lastname: Sui
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Jibran
authors.initials: J
authors.lastname: Iqbal
authors.forename: Yiping
authors.initials: Y
authors.lastname: Du
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003102:Colloids / Q000737:chemistry; D057230:Limit of Detection; D012275:Oryza / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D011725:Pyridines / Q000032:analysis; D015203:Reproducibility of Results; D012834:Silver / Q000032:analysis / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013844:Thiazoles / Q000032:analysis*
chemical_list: D003102:Colloids; D010573:Pesticide Residues; D011725:Pyridines; D013844:Thiazoles; D012834:Silver; C023666:pyridine; C041191:tricyclazole