title: Salt stress triggers enhanced cycling of Arabidopsis root plasma-membrane aquaporins.
abstract: Aquaporins of the plasma membrane intrinsic protein (PIP) subfamily are channels which facilitate the diffusion of water across the plant plasma membrane (PM). Although PIPs have been considered as canonical protein markers of this compartment, their endomembrane trafficking is still not well documented. We recently obtained insights into the constitutive cycling of PIPs in Arabidopsis root cells by means of fluorescence recovery after photobleaching (FRAP). This work also uncovered the behavior of the model isoform AtPIP2;1 in response to NaCl. The present addendum connects these findings to another recent work which describes the dynamic properties of AtPIP2;1 in the PM in normal and salt stress conditions by means of single particle tracking (SPT) and fluorescence correlation spectroscopy (FCS). The results suggest that membrane rafts play an important role in the partitioning of AtPIP2;1 in normal conditions and that clathrin-mediated endocytosis is predominant. In salt stress conditions, the rate of AtPIP2;1 cycling was enhanced and endocytosis was cooperated by a membrane raft-associated salt-induced pathway and a clathrin-dependent pathway.
authors.affiliation: Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Martinière
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Li
authors.forename: John
authors.initials: J
authors.lastname: Runions
authors.forename: Jinxing
authors.initials: J
authors.lastname: Lin
authors.forename: Christophe
authors.initials: C
authors.lastname: Maurel
authors.forename: Doan-Trung
authors.initials: DT
authors.lastname: Luu
mesh_terms: D020346:Aquaporins / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D004705:Endocytosis / Q000187:drug effects; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D020346:Aquaporins; D029681:Arabidopsis Proteins; D012965:Sodium Chloride