title: The anticaries effect of a food extract (shiitake) in a short-term clinical study.
abstract: The main objective was to investigate whether low-molecular-weight fraction of edible mushroom shiitake extract (Lentinus edodes) possesses caries-preventive properties. The study was designed as a double-blind, three-leg, cross-over, randomized, controlled clinical trial carried out on two series of volunteers at the University of Gothenburg, and the Academic Centre for Dentistry Amsterdam. Volunteers rinsed twice daily with a solution containing low-molecular-weight fraction of edible mushroom, placebo (negative control without active ingredients), or Meridol (positive control, AmF-SnF(2)) for two weeks, with a two-week washout period between each rinsing period. Changes in the acidogenicity of dental plaque before and after a sucrose challenge, shifts in microbial composition, and plaque scores were determined. Frequent rinses with shiitake reduced the metabolic activity of dental plaque. No reduction of plaque scores and no inhibition of the production of organic acids in plaque was found. Minor differences in microbial composition between test sessions were found. To conclude, the results indicate that shiitake extract has anticariogenic potential, but not to the same extent as the positive control.
authors.affiliation: Department of Cariology, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. peter.lingstrom@odontologi.gu.se
authors.forename: Peter
authors.initials: P
authors.lastname: Lingström
authors.forename: Egija
authors.initials: E
authors.lastname: Zaura
authors.forename: Haidar
authors.initials: H
authors.lastname: Hassan
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Buijs
authors.forename: Pamie
authors.initials: P
authors.lastname: Hedelin
authors.forename: Jonathan
authors.initials: J
authors.lastname: Pratten
authors.forename: David
authors.initials: D
authors.lastname: Spratt
authors.forename: Maria
authors.initials: M
authors.lastname: Daglia
authors.forename: Aneta
authors.initials: A
authors.lastname: Karbowiak
authors.forename: Caterina
authors.initials: C
authors.lastname: Signoretto
authors.forename: Martijn
authors.initials: M
authors.lastname: Rosema
authors.forename: Fridus
authors.initials: F
authors.lastname: van der Weijden
authors.forename: Michael
authors.initials: M
authors.lastname: Wilson
mesh_terms: D000328:Adult; D000588:Amines / Q000008:administration & dosage; D000704:Analysis of Variance; D001419:Bacteria / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D002327:Cariostatic Agents / Q000494:pharmacology*; D003773:Dental Plaque / Q000737:chemistry / Q000188:drug therapy* / Q000382:microbiology; D004311:Double-Blind Method; D004338:Drug Combinations; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D009067:Mouthwashes / Q000008:administration & dosage* / Q000737:chemistry; D012463:Saliva / Q000187:drug effects / Q000382:microbiology; D020942:Shiitake Mushrooms / Q000737:chemistry*; D013395:Sucrose; D011795:Surveys and Questionnaires; D014002:Tin Fluorides / Q000008:administration & dosage
chemical_list: D000588:Amines; D002327:Cariostatic Agents; D004338:Drug Combinations; D009067:Mouthwashes; D014002:Tin Fluorides; C059813:Meridol; D013395:Sucrose