title: Immunoglobulin heavy-chain fluorescence in situ hybridization-chromogenic in situ hybridization DNA probe split signal in the clonality assessment of lymphoproliferative processes on cytological samples.
abstract: BACKGROUND
  The human immunoglobulin heavy-chain (IGH) locus at chromosome 14q32 is frequently involved in different translocations of non-Hodgkin lymphoma (NHL), and the detection of any breakage involving the IGH locus should identify a B-cell NHL. The split-signal IGH fluorescence in situ hybridization-chromogenic in situ hybridization (FISH-CISH) DNA probe is a mixture of 2 fluorochrome-labeled DNAs: a green one that binds the telomeric segment and a red one that binds the centromeric segment, both on the IGH breakpoint. In the current study, the authors tested the capability of the IGH FISH-CISH DNA probe to detect IGH translocations and diagnose B-cell lymphoproliferative processes on cytological samples.
  
  
  METHODS
  Fifty cytological specimens from cases of lymphoproliferative processes were tested using the split-signal IGH FISH-CISH DNA probe and the results were compared with light-chain assessment by flow cytometry (FC), IGH status was tested by polymerase chain reaction (PCR), and clinicohistological data.
  
  
  RESULTS
  The signal score produced comparable results on FISH and CISH analysis and detected 29 positive, 15 negative, and 6 inadequate cases; there were 29 true-positive cases (66%), 9 true-negative cases (20%), 6 false-negative cases (14%), and no false-positive cases (0%). Comparing the sensitivity of the IGH FISH-CISH DNA split probe with FC and PCR, the highest sensitivity was obtained by FC, followed by FISH-CISH and PCR.
  
  
  CONCLUSIONS
  The split-signal IGH FISH-CISH DNA probe is effective in detecting any translocation involving the IGH locus. This probe can be used on different samples from different B-cell lymphoproliferative processes, although it is not useful for classifying specific entities. Cancer (Cancer Cytopathol) 2012;. © 2012 American Cancer Society.
authors.affiliation: Department of Medicine and Surgery, University of Salerno, Salerno, Italy. pzeppa@unisa.it
authors.forename: Pio
authors.initials: P
authors.lastname: Zeppa
authors.forename: Laura Virginia
authors.initials: LV
authors.lastname: Sosa Fernandez
authors.forename: Immacolata
authors.initials: I
authors.lastname: Cozzolino
authors.forename: Valentina
authors.initials: V
authors.lastname: Ronga
authors.forename: Rita
authors.initials: R
authors.lastname: Genesio
authors.forename: Maria
authors.initials: M
authors.lastname: Salatiello
authors.forename: Marco
authors.initials: M
authors.lastname: Picardi
authors.forename: Umberto
authors.initials: U
authors.lastname: Malapelle
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Troncone
authors.forename: Elena
authors.initials: E
authors.lastname: Vigliar
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D002863:Chromogenic Compounds / Q000737:chemistry; D002999:Clone Cells / Q000378:metabolism / Q000473:pathology; D003581:Cytodiagnosis / Q000379:methods; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics*; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018442:Lymphoma, B-Cell, Marginal Zone / Q000175:diagnosis / Q000235:genetics; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis / Q000235:genetics; D008232:Lymphoproliferative Disorders / Q000145:classification / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014178:Translocation, Genetic; D055815:Young Adult
chemical_list: D002863:Chromogenic Compounds; D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains