title: Genotyping of -374A/T, -429A/G, and 63 bp Ins/del polymorphisms of RAGE by rapid one-step hexaprimer amplification refractory mutation system polymerase chain reaction in breast cancer patients.
abstract: Several studies have focused on the RAGE genetic background and have demonstrated that its polymorphisms affect the receptor's activity, expression, and downstream signaling. However, there is only little information regarding RAGE polymorphism in breast cancer. In the present study, the authors studied RAGE polymorphisms in 71 patients with breast cancer and 93 healthy women. RAGE -374T/A, -429T/C, and 63 bp Ins/del polymorphisms were analyzed using a hexaprimer amplification refractory mutation system PCR (H-ARMS-PCR). The results showed that RAGE polymorphisms are not associated with breast cancer in the current study population. Larger studies are required to confirm these data in other populations.
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. mhd.hashemi@gmail.com
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemi
authors.forename: Abdolkarim
authors.initials: A
authors.lastname: Moazeni-Roodi
authors.forename: Farshid
authors.initials: F
authors.lastname: Arbabi
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Fazaeli
authors.forename: Ebrahim Eskandari
authors.initials: EE
authors.lastname: Nasab
authors.forename: Mohsen
authors.initials: M
authors.lastname: Taheri
authors.forename: Claus
authors.initials: C
authors.lastname: Kerkhoff
authors.forename: Saeid
authors.initials: S
authors.lastname: Ghavami
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D060005:Genotyping Techniques / Q000379:methods*; D017127:Glycation End Products, Advanced / Q000235:genetics*; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics*; D013997:Time Factors
chemical_list: D017931:DNA Primers; D017127:Glycation End Products, Advanced