title: Effects of HMG on revascularization and follicular survival in heterotopic autotransplants of mouse ovarian tissue.
abstract: Ovarian tissue transplantation is now considered as a procedure to preserve the fertility of young women patients undergoing cancer therapy. The present study investigated the effects and mechanism of human menopausal gonadotrophin (HMG) intervention on vascular remoulding in ovarian heterotopic autotransplantation. Ovaries of 8-week-old mice were cultured in vitro with different concentrations of HMG for 3h for measuring the expression of vascular endothelial growth factor (VEGF). The cultured ovaries were implanted under the kidney capsule and removed 24, 36, 48 h or 1 month after transplantation. Revascularization, fluid exudation and the number of surviving ovarian follicles were observed. The results showed that VEGF was increased 1.6-6.5 times in the HMG intervention groups. Revascularization appeared 24-36 h after transplantation and was earlier than that of the control. Fluid exudation increased incrementally with increasing HMG concentrations. The total number of surviving ovarian follicles was increased by 1.2-1.5 times in the HMG 0.15 IU/ml group as compared with the other groups 1 month after transplantation. It is concluded that intervention with HMG in vitro before transplantation could improve the blood supply reconstruction and survival of the autotransplanted ovarian follicles, which might be associated with increased VEGF expression.
authors.affiliation: Key Laboratory of Reproduction and Genetic of Ningxia Hui Autonomous Region, Department of Histology and Embryology in Ningxia Medical University, Yinchuan, People's Republic of China. 4083304@163.com
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Chang
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Ling
authors.initials: L
authors.lastname: Dang
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Ma
authors.forename: Changchun
authors.initials: C
authors.lastname: Hei
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Shen
authors.forename: Chengjun
authors.initials: C
authors.lastname: Zhao
authors.forename: Yufang
authors.initials: Y
authors.lastname: Cai
authors.forename: Xiuying
authors.initials: X
authors.lastname: Pei
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Zhang
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D030762:Estrous Cycle; D005260:Female; D008596:Menotropins / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000502:physiology; D009924:Organ Culture Techniques; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D010053:Ovary / Q000098:blood supply* / Q000378:metabolism / Q000637:transplantation*; D014182:Transplantation, Autologous; D019737:Transplants; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D042461:Vascular Endothelial Growth Factor A; D008596:Menotropins