title: Improvement of defective cystic fibrosis airway epithelial wound repair after CFTR rescue.
abstract: Airway damage and remodelling are important components of lung pathology progression in cystic fibrosis (CF). Although repair mechanisms are engaged to restore the epithelial integrity, these processes are obviously insufficient to maintain lung function in CF airways. Our aims were therefore to study how the basic cystic fibrosis transmembrane conductance regulator (CFTR) defect could impact epithelial wound healing and to determine if CFTR correction could improve it. Wound-healing experiments, as well as cell migration and proliferation assays, were performed to study the early phases of epithelial repair in human CF and non-CF airway cells. CFTR function was evaluated using CFTR small interferring (si)RNA and inhibitor GlyH101 in non-CF cells, and conversely after CFTR rescue with the CFTR corrector VRT-325 in CF cells. Wound-healing experiments first showed that airway cells from CF patients repaired slower than non-CF cells. CFTR inhibition or silencing in non-CF primary airway cells significantly inhibited wound closure. GlyH101 also decreased cell migration and proliferation. Interestingly, wild-type CFTR transduction in CF airway cell lines or CFTR correction with VRT-325 in CFBE-ΔF508 and primary CF bronchial monolayers significantly improved wound healing. Altogether our results demonstrated that functional CFTR plays a critical role in wound repair, and CFTR correction may represent a novel strategy to promote the airway repair processes in CF.
authors.affiliation: Centre de Recherche Centre Hospitalier de l'Université de Montréal, Département de médecine, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Nguyen Thu Ngan
authors.initials: NT
authors.lastname: Trinh
authors.forename: Olivier
authors.initials: O
authors.lastname: Bardou
authors.forename: Anik
authors.initials: A
authors.lastname: Privé
authors.forename: Emilie
authors.initials: E
authors.lastname: Maillé
authors.forename: Damien
authors.initials: D
authors.lastname: Adam
authors.forename: Sarah
authors.initials: S
authors.lastname: Lingée
authors.forename: Pasquale
authors.initials: P
authors.lastname: Ferraro
authors.forename: Martin-Yvon
authors.initials: MY
authors.lastname: Desrosiers
authors.forename: Christelle
authors.initials: C
authors.lastname: Coraux
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Brochiero
mesh_terms: D000328:Adult; D001980:Bronchi / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics* / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000382:microbiology; D008171:Lung Diseases / Q000382:microbiology; D016040:Lung Transplantation / Q000379:methods; D009154:Mutation; D034741:RNA, Small Interfering / Q000378:metabolism; D012038:Regeneration; D020545:Respiratory Mucosa / Q000378:metabolism; D013997:Time Factors; D014945:Wound Healing
chemical_list: C505032:CFTR protein, human; D034741:RNA, Small Interfering; D019005:Cystic Fibrosis Transmembrane Conductance Regulator