title: Amino-functionalized monolithic spin-type columns for high-throughput lectin affinity chromatography of glycoproteins.
abstract: Hydrophilic poly(ethylene glycol)-based monoliths were synthesized in the spin-tip format for high-throughput applications via pulsed electron beam irradiation. Monoliths with a homogeneous porous structure and a total porosity of 69% were obtained. The cross-linked polymeric structure was further mechanically stabilized via the incorporation of silica nanoparticles. Amino-functionalization of the monoliths was accomplished by a straightforward, water-based, one-step approach that entailed the electron-beam irradiation-induced grafting of poly(allylamine). The amine functionalized spin columns showed very low unspecific protein adsorption and were successfully applied as adsorbents in lectin affinity chromatography for the purification of ovalbumin. The novel columns also outperformed a commercially available system.
authors.affiliation: Leibniz-Institut für Oberflächenmodifizierung, Leipzig, Germany. senta.reichelt@iom-leipzig.de
authors.forename: Senta
authors.initials: S
authors.lastname: Reichelt
authors.forename: Christian
authors.initials: C
authors.lastname: Elsner
authors.forename: Andrea
authors.initials: A
authors.lastname: Prager
authors.forename: Sergei
authors.initials: S
authors.lastname: Naumov
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kuballa
authors.forename: Michael R
authors.initials: MR
authors.lastname: Buchmeiser
mesh_terms: D000327:Adsorption; D000818:Animals; D002645:Chickens; D002846:Chromatography, Affinity; D037102:Lectins / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010047:Ovalbumin / Q000302:isolation & purification*; D011073:Polyamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D037102:Lectins; D011073:Polyamines; C063994:polyallylamine; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D010047:Ovalbumin