title: Studies of histidine residues in soybean (Glycine max) urease.
abstract: Soybean urease has been investigated extensively to reveal the presence of histidine residue (s) in the active site and their potential role in the catalysis. The spectrophotometric studies using diethylpyrocarbonate (DEP) showed the modification of 11.76 ± 0.1 histidine residues per mole of native urease. Therefore, the results are indicative of the presence of twelve histidine residues per urease molecule. It is presumed that the soybean urease, being a hexameric protein possess two histidine residues per subunit. Correlation plot showed that the complete inactivation of soybean urease corresponds to the modification of 1.97 histidine residues per subunit. Further, double logarithmic plot of kapp versus DEP concentration has resulted in a linear correlation and thereby demonstrating that only one of the two histidine residues per subunit is catalytically essential. Significant protection has been observed against inactivation when urea or acetohydroxamate (AHA) is incubated with DEP treated urease. The studies have demonstrated the presence of one histidine residue at the active site of soybean urease and its significance in catalysis.
authors.affiliation: School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.forename: Arvind M
authors.initials: AM
authors.lastname: Kayastha
mesh_terms: D020134:Catalytic Domain; D004047:Diethyl Pyrocarbonate / Q000737:chemistry / Q000378:metabolism; D006639:Histidine / Q000737:chemistry*; D006877:Hydroxamic Acids / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D012639:Seeds / Q000737:chemistry / Q000201:enzymology; D030262:Soybean Proteins / Q000737:chemistry* / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry / Q000201:enzymology*; D014510:Urease / Q000737:chemistry* / Q000378:metabolism
chemical_list: D006877:Hydroxamic Acids; D030262:Soybean Proteins; D006639:Histidine; C006358:acetohydroxamic acid; D014510:Urease; D004047:Diethyl Pyrocarbonate