title: Simultaneous determination of drugs in human autopsy material using phase-optimized liquid chromatography.
abstract: In legal medicine in many cases drugs are detected in autopsy material without connection to the cause of death, and until now no further investigations have taken place. In our study more than 50 drugs were measured directly in several compartments. The deceased had received continual therapeutic treatment, treatment during an operation or an unsuccessful emergency therapy. Liquid-liquid extraction and an LC-MS/MS method were developed for the determination of these drug concentrations. When measuring many transitions in a biological matrix, two problems should be excluded: ion suppression and too few measurement points per peak. A relatively short operation time and sufficient separation were achieved by column, eluent and gradient optimization with POPLC (phase-optimized liquid chromatography). Various autopsy materials from about 170 cases were investigated. In particular, in nine cases with four or more simultaneously determined drugs, their distribution in the compartments is very interesting for pharmacokinetic examinations. The distribution patterns of the drugs in the compartments of one individual deceased were compared. This meant that the great differences between subjects that are normally encountered these studies could be excluded. Measurements of drug concentrations in human autopsy material deepens knowledge of the respective drugs' pharmacokinetics.
authors.affiliation: Institute of Clinical Pharmacology, Medical Faculty Carl Gustav Carus, Technical University Dresden, Fiedlerstr. 27, 01307 Dresden, Germany. reinhard.oertel@tu-dresden.de
authors.forename: R
authors.initials: R
authors.lastname: Oertel
authors.forename: J
authors.initials: J
authors.lastname: Pietsch
authors.forename: N
authors.initials: N
authors.lastname: Arenz
authors.forename: S G
authors.initials: SG
authors.lastname: Zeitz
authors.forename: L
authors.initials: L
authors.lastname: Goltz
authors.forename: W
authors.initials: W
authors.lastname: Kirch
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001344:Autopsy / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004358:Drug Therapy; D005260:Female; D006801:Humans; D059625:Liquid-Liquid Extraction; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000008:administration & dosage / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D013405:Suicide; D053719:Tandem Mass Spectrometry / Q000379:methods; D014018:Tissue Distribution
chemical_list: D004364:Pharmaceutical Preparations