title: [Exploring the three-dimensional structure of dermal tissues of normal skin and scar in rat with synchrotron radiation X-ray imaging technology].
abstract: OBJECTIVE
  To compare the morphological difference between dermal tissue of normal skin and that of scar in rat, and to explore its structural pattern.
  
  
  METHODS
  The full-thickness skin and the scar tissue formed 3 weeks after wound healing from SD rats were harvested as samples, which were prepared appropriately afterwards. Samples were scanned and imaged with synchrotron radiation technology, micro-CT, and phase-contrast imaging technology. The images were rebuilt with three-dimensional software.
  
  
  RESULTS
  The micro-CT was materialized by using X-ray generated by synchrotron radiation light source. The structure of dermal tissues was clearly shown with the assistance of phase-contrast imaging technology in the process. It was demonstrated that the dermal tissues of normal skin of rat were mainly composed of collagenous fibers, which twined together to form an olive-like structure. These olive-like structures as basic units were arranged randomly in a certain way. The collagenous fibers in dermal tissue of the scar were arranged in a parallel manner, while some fibers were crooked and arranged in a disorderly manner.
  
  
  CONCLUSIONS
  Dermal tissue of normal skin in rat has stable three-dimensional structure, and its basic structure and manner of composition are obviously different from those of scar dermal tissue.
authors.affiliation: Shanghai Institute of Burns, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Yu-zhi
authors.initials: YZ
authors.lastname: Jiang
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Tong
authors.forename: Ti-qiao
authors.initials: TQ
authors.lastname: Xiao
authors.forename: Hong-lan
authors.initials: HL
authors.lastname: Xie
authors.forename: Chun
authors.initials: C
authors.lastname: Qing
authors.forename: Guo-hao
authors.initials: GH
authors.lastname: DU
authors.forename: Shu-liang
authors.initials: SL
authors.lastname: Lu
mesh_terms: D000818:Animals; D002921:Cicatrix / Q000000981:diagnostic imaging*; D020405:Dermis / Q000000981:diagnostic imaging* / Q000473:pathology; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008297:Male; D008858:Microscopy, Phase-Contrast; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000000981:diagnostic imaging; D017356:Synchrotrons; D014057:Tomography, X-Ray Computed; D014945:Wound Healing