title: Transcutaneous immunization using a dissolving microneedle array protects against tetanus, diphtheria, malaria, and influenza.
abstract: Transcutaneous immunization (TCI) is an attractive alternative vaccination route compared to the commonly used injection systems. We previously developed a dissolving microneedle array for use as a TCI device, and reported that TCI with the dissolving microneedle array induced an immune response against model antigens. In the present study, we investigated the vaccination efficacy against tetanus and diphtheria, malaria, and influenza using this vaccination system. Our TCI system induced substantial increases in toxoid-specific IgG levels and toxin-neutralizing antibody titer and induced the production of anti-SE36 IgG, which could bind to malaria parasite. On influenza HA vaccination, robust antibody production was elicited in mice that provided complete protection against a subsequent influenza virus challenge. These findings demonstrate that TCI using a dissolving microneedle array can elicit large immune responses against infectious diseases. Based on these results, we are now preparing translational research for human clinical trials.
authors.affiliation: Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0781, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Matsuo
authors.forename: Sachiko
authors.initials: S
authors.lastname: Hirobe
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Yokota
authors.forename: Yurika
authors.initials: Y
authors.lastname: Ayabe
authors.forename: Masashi
authors.initials: M
authors.lastname: Seto
authors.forename: Ying-Shu
authors.initials: YS
authors.lastname: Quan
authors.forename: Fumio
authors.initials: F
authors.lastname: Kamiyama
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tougan
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Horii
authors.forename: Yohei
authors.initials: Y
authors.lastname: Mukai
authors.forename: Naoki
authors.initials: N
authors.lastname: Okada
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Nakagawa
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage; D004167:Diphtheria Toxin / Q000008:administration & dosage; D004168:Diphtheria Toxoid / Q000008:administration & dosage*; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000008:administration & dosage; D007114:Immunization / Q000295:instrumentation* / Q000379:methods; D007252:Influenza Vaccines / Q000008:administration & dosage*; D017780:Malaria Vaccines / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009339:Needles; D051381:Rats; D054772:Rats, Hairless; D013744:Tetanus Toxin / Q000008:administration & dosage; D013745:Tetanus Toxoid / Q000008:administration & dosage*
chemical_list: D000953:Antigens, Protozoan; D004167:Diphtheria Toxin; D004168:Diphtheria Toxoid; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D017780:Malaria Vaccines; D013744:Tetanus Toxin; D013745:Tetanus Toxoid