title: Potato disc bioassay and cytotoxic effect of Leptadenia pyrotechnica: comparative study of diverse extracts.
abstract: Comparative acute toxicity studies of the latex and sequential extracts of Leptadenia pyrotechnica (Forsk.) Decne (Asclepiadaceae) were recorded using brine shrimp. The higher toxicities were exhibited in latex; methanol, methanol/dichloromethane (1:1), defatted methanol/dichloromethane (1:1), defatted methanol and dichloromethane extracts. The other extracts; aqueous, alkaloids, ethyl acetate and n-butanol exhibited less toxicities compared with the other extracts. The estimated LC50 and its 95% confidence limits for these extracts expressed in ppm were: methanol, latex 18.84 (11.22-31.61), methanol/dichloromethane 19.95 (7.76-53.70), defatted methanol/dichloromethane 21.38 (7.24-63.10), defatted methanol 28.19 (16.27-48.81) and dichloromethane 30.90 (11.75-79.43). The anti-tumor activities; potato disc assays of methanol, ethyl acetate and alkaloids extracts showed good activities as anti-tumor agent which represented-49.30,-43.20 an -33.60%, respectively. While latex and aqueous extract represented-30.80 and-28.17%, respectively.
authors.forename: Amal M Youssef
authors.initials: AM
authors.lastname: Moustafa
authors.forename: Ahmed I
authors.initials: AI
authors.lastname: Khodair
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Saleh
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D000470:Alkaloids / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity; D029062:Apocynaceae / Q000737:chemistry* / Q000633:toxicity*; D001156:Artemia / Q000187:drug effects; D007840:Latex / Q000737:chemistry / Q000633:toxicity; D000432:Methanol / Q000737:chemistry; D008752:Methylene Chloride / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D011198:Solanum tuberosum / Q000737:chemistry*; D018675:Toxicity Tests
chemical_list: D000085:Acetates; D000470:Alkaloids; D000970:Antineoplastic Agents; D007840:Latex; D010936:Plant Extracts; D008752:Methylene Chloride; C007650:ethyl acetate; D020001:1-Butanol; D000432:Methanol