title: Quantitative 3D imaging of yeast by hard X-ray tomography.
abstract: Full-field hard X-ray tomography could be used to obtain three-dimensional (3D) nanoscale structures of biological samples. The image of the fission yeast, Schizosaccharomyces pombe, was clearly visualized based on Zernike phase contrast imaging technique and heavy metal staining method at a spatial resolution better than 50 nm at the energy of 8 keV. The distributions and shapes of the organelles during the cell cycle were clearly visualized and two types of organelle were distinguished. The results for cells during various phases were compared and the ratios of organelle volume to cell volume can be analyzed quantitatively. It showed that the ratios remained constant between growth and division phase and increased strongly in stationary phase, following the shape and size of two types of organelles changes. Our results demonstrated that hard X-ray microscopy was a complementary method for imaging and revealing structural information for biological samples.
authors.affiliation: National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui 230029, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zheng
authors.forename: Wenjie
authors.initials: W
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Guan
authors.forename: Xiangxia
authors.initials: X
authors.lastname: Song
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Yangchao
authors.initials: Y
authors.lastname: Tian
mesh_terms: D021621:Imaging, Three-Dimensional / Q000379:methods*; D019216:Metals, Heavy / Q000378:metabolism; D008858:Microscopy, Phase-Contrast / Q000379:methods; D015388:Organelles / Q000737:chemistry / Q000648:ultrastructure; D012568:Schizosaccharomyces / Q000737:chemistry* / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods; D055114:X-Ray Microtomography / Q000379:methods*
chemical_list: D019216:Metals, Heavy