title: Application of custom-made bioresorbable raw particulate hydroxyapatite/poly-L-lactide mesh tray with particulate cellular bone and marrow and platelet-rich plasma for a mandibular defect: evaluation of tray fit and bone quality in a dog model.
abstract: The purpose of this study was to evaluate tray fit and bone quality of particulate cancellous bone and marrow (PCBM) mandibular reconstruction using custom-made bioresorbable forged composites of a raw particulate hydroxyapatite/poly-L-lactide (HA/PLLA) tray in a dog model. Mesh sheets of HA/PLLA were formed in a tray shape according to the mandible stereolithographs of 14 beagle dogs. Platelet-rich plasma (PRP) was obtained from venous blood, and PCBM was harvested from the iliac crest. Bone defects were made bilaterally on the lower borders of the mandible. The PCBM and PRP were mixed and compressed into the defects and a custom-made HA/PLLA or a manually adopted titanium tray was fixed by screws. Tray fit and bone qualities were evaluated using computed tomography, microfocus computed tomography and confocal laser scanning microscopy. In buccal side, there is no significant difference with tray fit between the HA/PLLA and the titanium type, but in lingual side, it was better in the HA/PLLA type than that of the Ti type. Bone volume fraction (BV/TV) had markedly increased on the HA/PLLA side at 12 months. In conclusion, the custom-made HA/PLLA tray was easily and accurately adapted to the mandible, and had achieved sufficient bone quality by 12 months.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tokyo Medical University, 6-7-1 Nishishinjuku Shinjuku-ku, Tokyo 160-0023, Japan. matsuoam@tokyo-med.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Matsuo
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Takahashi
authors.forename: Harutsugi
authors.initials: H
authors.lastname: Abukawa
authors.forename: Daichi
authors.initials: D
authors.lastname: Chikazu
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D015519:Bone Density / Q000502:physiology; D016026:Bone Marrow Transplantation / Q000379:methods*; D001863:Bone Screws; D016025:Bone Transplantation / Q000379:methods*; D017076:Computer-Aided Design; D004195:Disease Models, Animal; D004285:Dogs; D017886:Durapatite / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008336:Mandibular Diseases / Q000601:surgery; D063175:Mandibular Reconstruction / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000379:methods; D010012:Osteogenesis / Q000502:physiology; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry*; D011474:Prosthesis Design; D013499:Surface Properties; D013526:Surgical Mesh; D014025:Titanium / Q000737:chemistry; D014057:Tomography, X-Ray Computed / Q000379:methods; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C499699:hydroxyapatite-polylactide; D017886:Durapatite; D014025:Titanium