title: Greater nucleic acids oxidation in the temporal lobe than the frontal lobe in SAMP8.
abstract: Our previous studies have shown that substantial amounts of 8-oxoguanine are present in the DNA and RNA in the hippocampi of old senescence-accelerated mice (SAMP8); however, oxidative damage to DNA and RNA in the other regions of the brain from a month after birth to the onset of aging has not been examined completely. In this study, we analyzed the amount of 8-oxoguanine in DNA and RNA in the temporal and frontal lobes of SAMP8 during aging by the immunohistochemical method. Compared with age-matched control acceleration-resistant mice (SAMR1), 8- and 12-month-old SAMP8 had increased amounts of 8-oxoguanine in the DNA and RNA in the frontal lobe, whereas in the temporal lobe, this trend began to appear as early as 4 months. The levels of 8-oxoguanine in the temporal lobe were significantly higher than those in the frontal lobe. These results indicate that nucleic acid oxidative damage occurs as an age-associated phenomenon, and can occur more easily in the temporal lobe than in the frontal lobe of SAMP8.
authors.affiliation: Graduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Gan
authors.forename: Ben
authors.initials: B
authors.lastname: Nie
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Takagi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hayakawa
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Sekiguchi
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Cai
mesh_terms: D000375:Aging / Q000235:genetics*; D019588:Aging, Premature / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000818:Animals; D004195:Disease Models, Animal; D005625:Frontal Lobe / Q000378:metabolism* / Q000503:physiopathology; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D051379:Mice; D008818:Mice, Neurologic Mutants; D009696:Nucleic Acids / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: C453560:7,8-dihydro-8-oxoguanine; D009696:Nucleic Acids; C024829:8-hydroxyguanine; D006147:Guanine