title: Triple-color super-resolution imaging of live cells: resolving submicroscopic receptor organization in the plasma membrane.
abstract: In living color: efficient intracellular covalent labeling of proteins with a photoswitchable dye using the HaloTag for dSTORM super-resolution imaging in live cells is described. The dynamics of cellular nanostructures at the plasma membrane were monitored with a time resolution of a few seconds. In combination with dual-color FPALM imaging, submicroscopic receptor organization within the context of the membrane skeleton was resolved.
authors.affiliation: Division of Biophysics, Department of Biology, University of Osnabrück, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Wilmes
authors.forename: Markus
authors.initials: M
authors.lastname: Staufenbiel
authors.forename: Domenik
authors.initials: D
authors.lastname: Lisse
authors.forename: Christian P
authors.initials: CP
authors.lastname: Richter
authors.forename: Oliver
authors.initials: O
authors.lastname: Beutel
authors.forename: Karin B
authors.initials: KB
authors.lastname: Busch
authors.forename: Samuel T
authors.initials: ST
authors.lastname: Hess
authors.forename: Jacob
authors.initials: J
authors.lastname: Piehler
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D003599:Cytoskeleton / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry; D014162:Transfection
chemical_list: D000199:Actins; D005456:Fluorescent Dyes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins