title: Genetic modification of baculovirus expression vectors.
abstract: As a protein expression vector, the baculovirus demonstrates many advantages over other vectors. With the development of biotechnology, baculoviral vectors have been genetically modified to facilitate high level expression of heterologous proteins in both insect and mammalian cells. These modifications include utilization of different promoters and signal peptides, deletion or replacement of viral genes for increasing protein secretion, integration of polycistronic expression cassette for producing protein complexes, and baculovirus pseudotyping, promoter accommodation or surface display for enhancing mammalian cell targeting gene delivery. This review summarizes the development and the current state of art of the baculovirus expression system. Further development of baculovirus expression systems will make them even more feasible and accessible for advanced applications.
authors.affiliation: State Key Laboratory of Virology and Joint-Lab of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Shu-fen
authors.initials: SF
authors.lastname: Li
authors.forename: Hua-lin
authors.initials: HL
authors.lastname: Wang
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Hu
authors.forename: Fei
authors.initials: F
authors.lastname: Deng
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D018014:Gene Transfer Techniques / Q000295:instrumentation; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D006801:Humans