title: Study of the anti-sapstain fungus activity of Bacillus amyloliquefaciens CGMCC 5569 associated with Ginkgo biloba and identification of its active components.
abstract: An endophytic bacterium, designated strain Bacillus amyloliquefaciens CGMCC 5569 was isolated from Chinese medicinal Ginkgo biloba collected from Xuzhou, China. Both the filtrate and the ethyl acetate extract of strain CGMCC 5569 showed growth inhibition activity against the sapstain fungi Lasiodiplodia rubropurpurea, L. crassispora, and L. theobromae obviously (>65%) based on the comparison of the length of zones on the petri dish. From the ethyl acetate extract of the filtrate, the antifungal compounds were obtained as a series of lipopeptides, which including series of fengycin, surfactin and bacillomycin. It showed strong growth inhibition activity in vitro against the L. rubropurpurea, L. crassispora and L. theobromae by about 70.22%, 69.53% and 78.76%, respectively. The strong anti-sapstain fungus activity indicated that the endophytic B. amyloliquefaciens CGMCC 5569 and its bioactive components might provide an alternative bio-resource for the bio-control of sapstain.
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yuan
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Sheng
authors.initials: S
authors.lastname: Qin
authors.forename: Gui-Hua
authors.initials: GH
authors.lastname: Zhao
authors.forename: You-Jian
authors.initials: YJ
authors.lastname: Feng
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Wei
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Jiang
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology*; D001203:Ascomycota / Q000166:cytology / Q000187:drug effects*; D001407:Bacillus / Q000145:classification / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D020441:Ginkgo biloba / Q000382:microbiology*; D055666:Lipopeptides / Q000378:metabolism* / Q000494:pharmacology*; D013045:Species Specificity
chemical_list: D000935:Antifungal Agents; D055666:Lipopeptides