title: [Association between H-ras and L-myc gene polymorphisms and susceptibility to colorectal cancer].
abstract: OBJECTIVE
  To explore the association between the polymorphisms of oncogenes H-ras and L-myc and colorectal cancer risk, and the interaction of those genes.
  
  
  METHODS
  The genotypes of H-ras and L-myc genes were determined by polymerase chain reaction-based restriction fragment length polymorphism analysis. Stratified analysis and logistic model were used to detect the gene-gene interaction. The gene-gene interaction was validated by multifactor dimensionality reduction (MDR) analysis.
  
  
  RESULTS
  The single SNP model showed that the polymorphisms of H-ras and L-myc genes were not significantly related with colorectal cancer risk (P > 0.05). Stratified analysis revealed that among the L-myc LS + SS genotype carriers, those with H-ras TC + CC genotype showed significantly increased risk of rectal cancer than those with TT genotype (OR = 1.81, P = 0.005). The positive interaction between L-myc and H-ras was detected by logistic regression model. The OR of the interaction effect was 2.74 (P = 0.024). This result was confirmed in the MDR model, with 54.83% testing balanced accuracy and 10/10 cross-validation consistency, and the model was still significant after the 1000 times permutation test (P = 0.001).
  
  
  CONCLUSIONS
  Our findings suggest that the polymorphism of H-ras and L-myc genes is not related to colorectal cancer risk, but there is a synergy between H-ras and L-myc polymorphisms in the development of rectal cancer.
authors.affiliation: Department of Epidemiology and Health Statistics, Zhejiang University, Hangzhou, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ni
authors.forename: Yong-jing
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Shan-chun
authors.initials: SC
authors.lastname: Zhang
authors.forename: Ming-juan
authors.initials: MJ
authors.lastname: Jin
authors.forename: Xin-yuan
authors.initials: XY
authors.lastname: Ma
authors.forename: Kai-yan
authors.initials: KY
authors.lastname: Yao
authors.forename: Qi-long
authors.initials: QL
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: Chen
mesh_terms: D000368:Aged; D003110:Colonic Neoplasms / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D016259:Genes, myc; D011905:Genes, ras; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D058011:Multifactor Dimensionality Reduction; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012004:Rectal Neoplasms / Q000235:genetics; D012306:Risk; D011795:Surveys and Questionnaires