title: Deletion of Ten-m3 induces the formation of eye dominance domains in mouse visual cortex.
abstract: The visual system is characterized by precise retinotopic mapping of each eye, together with exquisitely matched binocular projections. In many species, the inputs that represent the eyes are segregated into ocular dominance columns in primary visual cortex (V1), whereas in rodents, this does not occur. Ten-m3, a member of the Ten-m/Odz/Teneurin family, regulates axonal guidance in the retinogeniculate pathway. Significantly, ipsilateral projections are expanded in the dorsal lateral geniculate nucleus and are not aligned with contralateral projections in Ten-m3 knockout (KO) mice. Here, we demonstrate the impact of altered retinogeniculate mapping on the organization and function of V1. Transneuronal tracing and c-fos immunohistochemistry demonstrate that the subcortical expansion of ipsilateral input is conveyed to V1 in Ten-m3 KOs: Ipsilateral inputs are widely distributed across V1 and are interdigitated with contralateral inputs into eye dominance domains. Segregation is confirmed by optical imaging of intrinsic signals. Single-unit recording shows ipsilateral, and contralateral inputs are mismatched at the level of single V1 neurons, and binocular stimulation leads to functional suppression of these cells. These findings indicate that the medial expansion of the binocular zone together with an interocular mismatch is sufficient to induce novel structural features, such as eye dominance domains in rodent visual cortex.
authors.affiliation: Discipline of Physiology, School of Medical Sciences and the Bosch Institute, University of Sydney, Sydney, New South Wales 2006, Australia.
authors.forename: Sam
authors.initials: S
authors.lastname: Merlin
authors.forename: Sam
authors.initials: S
authors.lastname: Horng
authors.forename: Lauren R
authors.initials: LR
authors.lastname: Marotte
authors.forename: Mriganka
authors.initials: M
authors.lastname: Sur
authors.forename: Atomu
authors.initials: A
authors.lastname: Sawatari
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Leamey
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D001931:Brain Mapping; D023882:Dominance, Ocular / Q000235:genetics*; D007839:Functional Laterality / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D008565:Membrane Proteins / Q000172:deficiency* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000172:deficiency* / Q000235:genetics; D009474:Neurons / Q000502:physiology; D010775:Photic Stimulation; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D014793:Visual Cortex / Q000166:cytology / Q000502:physiology*; D014795:Visual Pathways / Q000033:anatomy & histology / Q000502:physiology*
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; C525233:Tenm3 protein, mouse