title: NMDAR2B tyrosine phosphorylation is involved in thermal nociception.
abstract: Previous studies found that the NMDA receptor-mediated signaling regulates thermal nociception, though the underlying molecular mechanism remains unclear. The GluN2B subunit of the NMDA receptor is tyrosine-phosphorylated, Tyr-1472 being the major phosphorylation site. In this study, we have found that homozygous knock-in mice that express a Tyr-1472-Phe mutant of GluN2B display defects in the nociceptive response in the hot plate test. Expression of the neurotensin receptor subtype 2 (NTSR2), which is relevant to the regulation of thermal nociception, is decreased in the amygdala of GluN2B Tyr-1472-Phe knock-in mice. In addition, NTSR2-mediated c-fos induction is impaired in the amygdala of these mice. These data suggest that Tyr-1472 phosphorylation on GluN2B is involved in thermal nociception through regulating the NTSR2 mRNA expression in the amygdala.
authors.affiliation: Division of Oncology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Mina
authors.initials: M
authors.lastname: Delawary
authors.forename: Tohru
authors.initials: T
authors.lastname: Tezuka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kiyama
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Etsuko
authors.initials: E
authors.lastname: Wada
authors.forename: Keiji
authors.initials: K
authors.lastname: Wada
authors.forename: Toshiya
authors.initials: T
authors.lastname: Manabe
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Takanobu
authors.initials: T
authors.lastname: Nakazawa
mesh_terms: D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D005786:Gene Expression Regulation; D055879:Gene Knock-In Techniques; D006358:Hot Temperature; D051379:Mice; D008810:Mice, Inbred C57BL; D059225:Nociception / Q000502:physiology*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism*; D018028:Receptors, Neurotensin / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
chemical_list: C121001:NR2B NMDA receptor; C490984:Ntsr2 protein, mouse; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D018028:Receptors, Neurotensin; D014443:Tyrosine