title: A focused immune response targeting the homotypic binding domain of the carcinoembryonic antigen blocks the establishment of tumor foci in vivo.
abstract: Metastatic forms of cancers remain the main cause of death in cancer patients. In this study, we demonstrate that directing a sustained antibody response towards the homotypic binding function of CEA interferes with the implantation and development of tumor foci in CEA-expressing transgenic (CEA.Tg) mice. Specifically, vaccinating CEA.Tg mice with a recombinant, altered self-form of the CEA Ig V-like N domain led to the production of circulating IgG1 and IgG2a antibodies that inhibited CEA-mediated adhesion of murine carcinoma expressing CEA (MC38.CEA) and mediated antibody-dependent lysis of tumor cells. Moreover, vaccinated CEA.Tg mice were resistant to the development of tumor nodules in the lungs and the peritoneal cavity, suggesting that mounting a focused antibody response to the CEA N domain may represent a simple therapeutic strategy to control the establishment of metastatic foci in cancer patients.
authors.affiliation: Department of Medical Biophysics, University of Toronto; Toronto, Ontario, Canada.
authors.forename: Aws
authors.initials: A
authors.lastname: Abdul-Wahid
authors.forename: Eric H-B
authors.initials: EH
authors.lastname: Huang
authors.forename: Huixin
authors.initials: H
authors.lastname: Lu
authors.forename: Jean
authors.initials: J
authors.lastname: Flanagan
authors.forename: Amirul Islam
authors.initials: AI
authors.lastname: Mallick
authors.forename: Jean
authors.initials: J
authors.lastname: Gariépy
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D051379:Mice; D009362:Neoplasm Metastasis / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology
chemical_list: D019496:Cancer Vaccines; D002272:Carcinoembryonic Antigen