title: Mesenchymal stem cell adherence on poly(D, L-lactide-co-glycolide) nanofibers scaffold is integrin-beta 1 receptor dependent.
abstract: Tissue engineering is a potential approach to regenerate damaged tissue by the combination and synergism among the scaffolding material, cell source and signaling factors. In the present study, mesenchymal stem cells (MSCs) were isolated from C57BL/6 mice, cultured on poly(D, L-lactide-co-glycolide) (PLGA) scaffold produced by electrospinning technique and differentiated into chondrogenic lineage. After seeding, MSCs were responsive and became flattened with fibroblast-like morphology demonstrated by the presence of actin stress fibers. Integrin-beta1 receptor blockage reduced significantly cell adhesion with loss of actin stress fibers, demonstrating the ability of PLGA nanofiber to trigger integrin receptor-mediated cell adhesion. Present data contribute to the understanding of MSCs' behavior on these biodegradable and biocompatible scaffolds that can be used as carriers in treatments involving cell transplantation.
authors.affiliation: Cerebral Ischemia Laboratory, Department of Biochemistry Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Prédio Anexo, 90035-003, Brazil.
authors.forename: Geancarlo
authors.initials: G
authors.lastname: Zanatta
authors.forename: Markus
authors.initials: M
authors.lastname: Rudisile
authors.forename: Melissa
authors.initials: M
authors.lastname: Camassola
authors.forename: Joachim
authors.initials: J
authors.lastname: Wendorff
authors.forename: Nance
authors.initials: N
authors.lastname: Nardi
authors.forename: Carmem
authors.initials: C
authors.lastname: Gottfried
authors.forename: Patricia
authors.initials: P
authors.lastname: Pranke
authors.forename: Carlos Alexandre
authors.initials: CA
authors.lastname: Netto
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005434:Flow Cytometry; D019012:Integrin beta1 / Q000378:metabolism*; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D057139:Nanofibers / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D015703:Antigens, CD; D001672:Biocompatible Materials; D019012:Integrin beta1; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid