title: Formation of GM1 ganglioside clusters on the lipid membrane containing sphingomyeline and cholesterol.
abstract: GM1 gangliosides form a microdomain with sphingomyeline (SM) and cholesterol (Chol) and are deeply involved in the aggregation of amyloid beta (Aβ) peptides on neural membranes. We performed molecular dynamics simulations on two kinds of lipid bilayers containing GM1 ganglioside: GM1/SM/Chol and GM1/POPC. Both 10 and 100 ns simulations and another set of 10 ns simulations with different initial lipid arrangement essentially showed the same computational results. GM1 molecules in the GM1/SM/Chol membrane were condensed, whereas those in GM1/POPC membrane scattered. That is, the formation of GM1 cluster was observed only on the GM1/SM/Chol mixed membrane. There appeared numerous hydrogen bonds among glycan portions of the GM1 clusters due to the condensation. A comparison in distribution of lipid molecules between the two kinds of membranes suggested that cholesterol had important roles to prevent the membrane from interdigitation and to stabilize other lipids for interacting with each other. This property of cholesterol promotes the formation of GM1 clusters.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Mori
authors.forename: Md Iqbal
authors.initials: MI
authors.lastname: Mahmood
authors.forename: Saburo
authors.initials: S
authors.lastname: Neya
authors.forename: Katsumi
authors.initials: K
authors.lastname: Matsuzaki
authors.forename: Tyuji
authors.initials: T
authors.lastname: Hoshino
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry*; D016000:Cluster Analysis; D005677:G(M1) Ganglioside / Q000737:chemistry*; D006860:Hydrogen Bonding; D008051:Lipid Bilayers / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D010713:Phosphatidylcholines / Q000737:chemistry; D013109:Sphingomyelins / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D005677:G(M1) Ganglioside; D002784:Cholesterol; C028694:1-palmitoyl-2-oleoylphosphatidylcholine