title: Crystallization and preliminary X-ray diffraction analysis of a novel wild-type blue fluorescent protein from Vibrio vulnificus CKM-1.
abstract: The use of green fluorescent protein (GFP) for non-invasive in vivo imaging is limited to aerobic systems, as chromophore formation requires oxygen. However, a novel NADPH-dependent blue fluorescent protein from Vibrio vulnificus CKM-1 (BFPvv) that emits blue fluorescence in both aerobic and anaerobic systems has recently been discovered. Wild-type BFPvv was overexpressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method. The resulting BFPvv crystals diffracted to a resolution of 1.9 Å and belonged to space group P3, with unit-cell parameters a = b = 96.62, c = 214.511 Å. Assuming the presence of eight molecules in the unit cell, the solvent content was estimated to be ~56.16%.
authors.affiliation: Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Chin-Feng
authors.initials: CF
authors.lastname: Liu
authors.forename: Chao-Hung
authors.initials: CH
authors.lastname: Kao
authors.forename: Yen-Chao
authors.initials: YC
authors.lastname: Su
authors.forename: Yeh
authors.initials: Y
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008164:Luminescent Proteins / Q000737:chemistry*; D041261:Vibrio vulnificus / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins