title: Proteomics and metabolomics of Arabidopsis responses to perturbation of glucosinolate biosynthesis.
abstract: To understand plant molecular networks of glucosinolate metabolism, perturbation of aliphatic glucosinolate biosynthesis was established using inducible RNA interference (RNAi) in Arabidopsis. Two RNAi lines were chosen for examining global protein and metabolite changes using complementary proteomics and metabolomics approaches. Proteins involved in metabolism including photosynthesis and hormone metabolism, protein binding, energy, stress, and defense showed marked responses to glucosinolate perturbation. In parallel, metabolomics revealed major changes in the levels of amino acids, carbohydrates, peptides, and hormones. The metabolomics data were correlated with the proteomics results and revealed intimate molecular connections between cellular pathways/processes and glucosinolate metabolism. This study has provided an unprecedented view of the molecular networks of glucosinolate metabolism and laid a foundation towards rationale glucosinolate engineering for enhanced defense and quality.
authors.affiliation: Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Ya-zhou
authors.initials: YZ
authors.lastname: Chen
authors.forename: Qiu-Ying
authors.initials: QY
authors.lastname: Pang
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Ning
authors.initials: N
authors.lastname: Zhu
authors.forename: Isabel
authors.initials: I
authors.lastname: Branstrom
authors.forename: Xiu-Feng
authors.initials: XF
authors.lastname: Yan
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D005961:Glucosinolates / Q000096:biosynthesis*; D055432:Metabolomics; D040901:Proteomics
chemical_list: D029681:Arabidopsis Proteins; D005961:Glucosinolates