title: Crystallization and preliminary crystallographic analysis of the complex between triiodothyronine and the bb' fragment of rat protein disulfide isomerase.
abstract: Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation of a disulfide bond in nascent and misfolded proteins and is also known to bind to the thyroid hormone triiodothyronine (T3). When T3 is bound to PDI its catalytic activity is inhibited, but the biological function of this binding is not well understood. In previous studies, it was found that T3 binds to the bb' fragment of PDI. Therefore, to clarify the structure of the complex consisting of PDI bound to T3, a crystallographic analysis of the three-dimensional structure of the T3-rat PDI bb' complex was performed. Native bb' crystals and T3-bb' complex crystals were both obtained using the hanging-drop vapour-diffusion technique with 1.6 M trisodium citrate pH 6.2 as a precipitant. The space group of the native bb' crystals was found to be C222, with unit-cell parameters a = 94.8, b = 114.9, c = 182.9 Å, while the space group of the T3-bb' complex crystals was P2(1)2(1)2(1), with unit-cell parameters a = 99.9, b = 184.5, c = 232.2 Å. Diffraction data for the native and complex crystals were collected to resolutions of 3.06 and 3.00 Å, respectively.
authors.affiliation: School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Len
authors.initials: L
authors.lastname: Ito
authors.forename: Masaki
authors.initials: M
authors.lastname: Okumura
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Shibano
authors.forename: Marina
authors.initials: M
authors.lastname: Nawata
authors.forename: Takashi
authors.initials: T
authors.lastname: Kumasaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Susumu
authors.initials: S
authors.lastname: Imaoka
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D011485:Protein Binding; D019704:Protein Disulfide-Isomerases / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D014284:Triiodothyronine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D014284:Triiodothyronine; D019704:Protein Disulfide-Isomerases