title: Characterization of nuclear import and export signals determining the subcellular localization of WD repeat-containing protein 42A (WDR42A).
abstract: WD repeat-containing protein 42A (WDR42A) is a member of the WD40-repeat proteins. Here, we investigated the localization pattern of WDR42A in living cells. By mutational analysis, a nuclear localization signal, 114PRRRVQRKR122, was for the first time determined. The dominant negative, co-immunoprecipitation and GST pull-down results further demonstrated that the nuclear import of WDR42A was mediated by karyopherin-α1/β1 in conjunction with the GTPase Ran. Additionally, a nuclear export signal, 39IEVEASDLSLSL50, was verified to be a functional NES, which mediated the nuclear export through Chromosome Region Maintenance 1 dependent pathway. All these data suggest WDR42A is a nucleocytoplasmic shuttling protein.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuchang, Wuhan, China.
authors.forename: Fuqing
authors.initials: F
authors.lastname: Wu
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Junji
authors.initials: J
authors.lastname: Xing
authors.forename: Meili
authors.initials: M
authors.lastname: Li
authors.forename: Chunfu
authors.initials: C
authors.lastname: Zheng
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology; D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D019913:Nuclear Localization Signals / Q000378:metabolism*; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D019913:Nuclear Localization Signals; C559948:WDR42 protein, human