title: [Extraction technology of diester-type alkaloids from Radix Aconiti lateralis].
abstract: OBJECTIVE
  To determine the suitable extraction technology of diester-type alkaloids, such as mesaconitine, aconitine and hypaconitine from the roots of Aconitum carmichaeli (Radix Aconiti lateralis).
  
  
  METHODS
  The contents of mesaconitine, aconitine and hypaconitine were determined by HPLC. Single-factor experiment was used to study the extraction factors.
  
  
  RESULTS
  The suitable extraction technology for diester-type alkaloids was as follows: coarse sizings of Radix Aconiti lateralis was extracted by 10 times ethanol for 3 times (each time for 1 day) at 15 degrees C. The total yield of diester-type alkaloids was 0.57%, including 0.16% aconitine, 0.032% mesaconitine and 0.38% hypaconitine, and the purity was 18.81%.
  
  
  CONCLUSIONS
  The extraction technology is efficient, harmfulless, economical, convenient and can be used for industrial production.
authors.affiliation: School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621000, China. liuzhuo19852009@163.com
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Liu
authors.forename: Xing
authors.initials: X
authors.lastname: Yan
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Xu
authors.forename: Da-Bin
authors.initials: DB
authors.lastname: Hou
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Yuan
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification*; D031841:Aconitum / Q000737:chemistry*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000431:Ethanol; C058309:hypaconitine; C019470:mesaconitine; D000157:Aconitine