title: DNA binding studies of 3, 5, 6-trichloro-2-pyridinol pesticide metabolite.
abstract: 3, 5, 6-Trichloro-2-pyridinol (TCP) is a stable metabolite of two major pesticides, Chlopyrifos insecticide and Triclopyr herbicide, which are widely used in the world. The potential health hazard associated with TCP is identified due to its high affinity to the DNA molecule. Therefore, in this study, the interaction of native calf thymus DNA with TCP has been investigated using spectrophotometric, circular dichroism (CD), spectrofluorometric, viscometric and voltametric techniques. It was found that TCP molecules could interact with DNA via a groove-binding mode, as evidenced by hyperchromism, with no red shift in the UV absorption band of TCP, no changes in K(b) values in the presence of salt, no significant changes in the specific viscosity and CD spectra of DNA, and a decrease in peak currents with no shift in the voltamogram. In addition, TCP is able to release Hoechst 33258, a strong groove binder, in the DNA solutions. The results are indicative of the groove-binding mode of TCP to DNA.
authors.affiliation: Faculty of Chemistry, Sensor and Biosensor Research Center & Nanoscience and Nanotechnology Research Center, Razi University, Kermanshah, Islamic Republic of Iran. kashanian_s@yahoo.com
authors.forename: Soheila
authors.initials: S
authors.lastname: Kashanian
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Shariati
authors.forename: Hamideh
authors.initials: H
authors.lastname: Roshanfekr
authors.forename: Sirous
authors.initials: S
authors.lastname: Ghobadi
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D006690:Bisbenzimidazole / Q000378:metabolism; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects; D004277:DNA, Single-Stranded / Q000378:metabolism; D004563:Electrochemistry; D010575:Pesticides / Q000378:metabolism* / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D011728:Pyridones / Q000378:metabolism* / Q000494:pharmacology; D014783:Viscosity
chemical_list: D004277:DNA, Single-Stranded; D010575:Pesticides; D011728:Pyridones; C012587:3,5,6-trichloro-2-pyridinol; D004247:DNA; D006690:Bisbenzimidazole