title: [Determination of aflatoxins in cashew by high performance liquid chromatography-tandem mass spectrometry].
abstract: A method for the determination of four aflatoxins in cashew using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The sample was extracted with methanol-water (8: 2, v/v) solution, followed by a cleanup procedure with Florisil column. The target compounds were eluted using 5 mL acetone-water-formic acid (96: 3.5:0.5, v/v/v) solution. The eluate was dried under N2, then dissolved in 1 mL methanol. Four aflatoxins were separated in MG C18 column (100 mm x 3.0 mm, 3 microm) adopting a gradient program within 15 min. A triple quadrupole mass spectrometry equipped with an electrospray ionization source operated in the positive ion mode was used to detect the aflatoxins. The good correlation coefficients (r2 > 0.997) of the four aflatoxins were obtained within their respective linear ranges. The limits of detection (S/N = 3) were between 0.009 microg/kg and 0.04 microg/kg, and the limits of quantification (S/N = 10) were between 0.03 microg/kg and 0.12 microg/kg. The recoveries were in a range of 63.0% -78.5% with the relative standard deviations (RSDs) varied from 2.8% to 9.1%. The validation results meet the requirements of trace assay. Matrix effects were estimated and the signal suppression/enhancement ranged from 88.8% to 99.4%. The results indicate that the developed method is simple, fast, accurate, and can be applied for the determination of fours aflatoxins in cashew.
authors.affiliation: College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. prefers@163.com
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Bi
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Fan
authors.forename: Meng
authors.initials: M
authors.lastname: Fu
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D031021:Anacardium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D000348:Aflatoxins