title: Ultrasensitive aptamer-based bio bar code immunomagnetic separation and electrochemiluminescence method for the detection of protein.
abstract: An ultrasensitive aptamer-based bio bar code immunomagnetic separation and electrochemiluminescence (IM-ECL) method for the detection of protein is developed. The target protein is captured by biotin-labeled aptamer (biotin probe) and [Ru(bpy)(3)](2+) (TBR)-Au bio bar code-labeled aptamer (ECL nanoprobe), to form a double aptamer-protein sandwich complex. The complex is then immobilized on the streptavidin microbeads through biotin-streptavidin linkage and detected by ECL assay. The ECL signal of the target protein is amplified by the TBR-bio bar code DNAs. As an example, platelet-derived growth factor B-chain homodimer (PDGF-BB) was detected by the method. Experimental results show that the detection limit of the assay is 1 pM of PDGF-BB. A calibration curve with a linearity range from 1 pM to 10 nM is established, thus, make quantitative analysis possible. The method has been used to detect PDGF-BB in fetal calf serum with minimum background interference. Due to the wide availability of aptamer for numerous proteins, this aptamer-based bio bar code IM-ECL method holds great promise in protein detection.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Debin
authors.initials: D
authors.lastname: Zhu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhou
authors.forename: Da
authors.initials: D
authors.lastname: Xing
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000737:chemistry; D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D000077214:Becaplermin; D015374:Biosensing Techniques; D001710:Biotin; D002417:Cattle; D056831:Coordination Complexes; D055664:Electrochemical Techniques; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry; D020574:Proto-Oncogene Proteins c-sis / Q000097:blood*; D012680:Sensitivity and Specificity; D019809:Streptavidin; D017975:Ruthenium Compounds
chemical_list: D015082:2,2'-Dipyridyl; D052157:Aptamers, Nucleotide; D000077214:Becaplermin; D001710:Biotin; D056831:Coordination Complexes; D006046:Gold; D020574:Proto-Oncogene Proteins c-sis; D019809:Streptavidin; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds