title: Identification of molecular target proteins in berberine-treated cervix adenocarcinoma HeLa cells by proteomic and bioinformatic analyses.
abstract: In this study, the apoptosis of HeLa cells induced by berberine was investigated. Fifty-one differentially expressed proteins were identified before and after berberine treatment by a proteomic method, which either interacted with each other directly or through one intermediate protein to form a connected protein interaction sub-network. Nine of them were selected and validated. Compared with the cells in the control group, the expressions of 14-3-3σ and lamin-A/C of the cells treated by berberine for 48 h increased by 94.12 and 5.24 times, respectively, and the expressions of annexin A5, cytokeratin 17, prohibitin, heat shock cognate 71 kDa protein (HSPA8), programmed cell death 6 and vimentin decreased by 4.1, 1.34, 23.8, 11.85, 4.63 and 5.24 times, respectively. In addition, tubulin-β decreased from 9537 to 6908 ng/L. Furthermore, the inverse dock program (INVDOCK) was used to predict the possible drug-target of berberine's anticancer activity, and the results showed that HSPA8 and annexin A5 might be the drug targets. This study suggests that the anticancer effect of berberine on HeLa cells was a complex process based on affecting multiple protein expression and acting on an interaction network. Our work could be helpful to elucidate the mechanism of berberine's anticancer activity on HeLa cells.
authors.affiliation: College of Pharmacy, Dalian Medical University, Dalian, 116044, China.
authors.forename: Binan
authors.initials: B
authors.lastname: Lu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Lina
authors.initials: L
authors.lastname: Xu
authors.forename: Yousong
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiaona
authors.initials: X
authors.lastname: Wang
authors.forename: Jinyong
authors.initials: J
authors.lastname: Peng
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001599:Berberine / Q000737:chemistry / Q000494:pharmacology*; D019295:Computational Biology / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D050883:HSC70 Heat-Shock Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D060066:Protein Interaction Maps; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D017304:Annexin A5; D000970:Antineoplastic Agents; D050883:HSC70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D011506:Proteins; D001599:Berberine