title: Combined hypermethylation of APC and GSTP1 as a molecular marker for prostate cancer: quantitative pyrosequencing analysis.
abstract: A total of 149 human prostate tissues obtained from our institute were assessed: 52 specimens of benign prostate hyperplasia (BPH) and 97 specimens of prostate cancer (PCa). The methylation status of the genes of Adenomatous polyposis coli (APC) and glutathione-S-transferase-P1 (GSTP1) was analyzed by quantitative pyrosequencing. A methylation score (M score) was calculated to capture the combined methylation level of both genes. The methylation level of each single gene and that of both genes combined was significantly higher in PCa specimens than in BPH (each p < 0.001). The value of APC methylation, GSTP1 methylation, and M score for predicting PCa was measured by the area under the receiver operating characteristic (ROC) curve and reached 0.954, 0.942, and 0.983, respectively. The sensitivity and specificity of the M score in discriminating between PCa and BPH reached 92.8% and 100.0%, respectively. The M score was positively associated with the serum prostate-specific antigen (PSA) level (p trend < 0.001). Our study demonstrates that the quantitative measurement of two methylation markers might drastically improve the ability to discriminate PCa from BPH.
authors.affiliation: Department of Urology, College of Medicine, Chungbuk National University, Cheongju, South Korea.
authors.forename: Hyung-Yoon
authors.initials: HY
authors.lastname: Yoon
authors.forename: Seon-Kyu
authors.initials: SK
authors.lastname: Kim
authors.forename: Young-Won
authors.initials: YW
authors.lastname: Kim
authors.forename: Ho Won
authors.initials: HW
authors.lastname: Kang
authors.forename: Sang-Cheol
authors.initials: SC
authors.lastname: Lee
authors.forename: Keun Ho
authors.initials: KH
authors.lastname: Ryu
authors.forename: Ho Sun
authors.initials: HS
authors.lastname: Shon
authors.forename: Wun-Jae
authors.initials: WJ
authors.lastname: Kim
authors.forename: Yong-June
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D000368:Aged; D019175:DNA Methylation; D017491:Genes, APC; D005819:Genetic Markers; D051549:Glutathione S-Transferase pi / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D011379:Prognosis; D017430:Prostate-Specific Antigen / Q000097:blood; D011470:Prostatic Hyperplasia / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers; D051549:Glutathione S-Transferase pi; D017430:Prostate-Specific Antigen