title: Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy.
abstract: Immunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the low-level detection of dendrimers, improving the limit of detection by a factor of 1000 (LOD = 2.5 × 10(-13) moles). The biorecognition method is reproducible and shows high specificity and good accuracy. In addition, the capture assay platform shows a promising approach to patterning dendrimers for nanotechnology applications.
authors.affiliation: Department of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USA.
authors.forename: Chevelle A
authors.initials: CA
authors.lastname: Cason
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Fabré
authors.forename: Andrew
authors.initials: A
authors.lastname: Buhrlage
authors.forename: Kristi L
authors.initials: KL
authors.lastname: Haik
authors.forename: Heather A
authors.initials: HA
authors.lastname: Bullen