title: Endogenous stem cell therapy enhances fat graft survival.
abstract: BACKGROUND
  Lipoaspirate centrifugation creates graded density of adipose tissue. High-density fat contains more vasculogenic cytokines and progenitor cells and has greater graft survival than low-density fat. The authors hypothesize that accelerating the bone marrow-derived progenitor cell response to injected low-density fat will improve its graft survival.
  
  
  METHODS
  Male 8-week-old FVB mice (n=60) were grafted with either high-density (n=20) or low-density (n=40) human lipoaspirate. Half of the mice receiving low-density fat (n=20) were treated with a stem cell mobilizer for 14 days. Grafted fat was harvested at 2 and 10 weeks for analysis.
  
  
  RESULTS
  Low-density fat, low-density fat plus daily AMD3100, and high-density fat had 26±3.0, 61.2±7.5, and 49.6±3.5 percent graft survival, respectively, at 2 weeks (low-density fat versus low-density fat plus daily AMD3100 and low-density fat versus high-density fat, both p<0.01). Similar results were observed 10 weeks after grafting. Mice receiving low-density fat plus daily AMD3100 had significantly more vasculogenic progenitor cells per cubic centimeter of peripheral blood (p<0.01) and more new blood vessels (p<0.01). Both low-density fat plus daily AMD3100 and high-density fat contained more stromal-derived factor-1α and vascular endothelial growth factor mRNA/protein.
  
  
  CONCLUSIONS
  Endogenous progenitor cell mobilization enhances low-density fat neovascularization, increases vasculogenic cytokine expression, and improves graft survival to a level equal to that of high-density fat grafts.
authors.affiliation: New York, N.Y. From the Department of Plastic Surgery, New York University Langone Medical Center.
authors.forename: Parag
authors.initials: P
authors.lastname: Butala
authors.forename: Alexes
authors.initials: A
authors.lastname: Hazen
authors.forename: Caroline
authors.initials: C
authors.lastname: Szpalski
authors.forename: Steven M
authors.initials: SM
authors.lastname: Sultan
authors.forename: Sydney R
authors.initials: SR
authors.lastname: Coleman
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Warren
mesh_terms: D052436:Adipose Tissue, White / Q000098:blood supply / Q000378:metabolism / Q000637:transplantation*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001596:Benzylamines; D015415:Biomarkers / Q000378:metabolism; D054377:Chemokine CXCL12 / Q000378:metabolism; D000080027:Cyclams; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006571:Heterocyclic Compounds / Q000008:administration & dosage*; D006801:Humans; D015187:Lipectomy; D008297:Male; D051379:Mice; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000378:metabolism; D042442:Vascular Endothelial Growth Factors / Q000378:metabolism; D055815:Young Adult
chemical_list: D001596:Benzylamines; D015415:Biomarkers; D054377:Chemokine CXCL12; D000080027:Cyclams; D006571:Heterocyclic Compounds; D042442:Vascular Endothelial Growth Factors; C088327:plerixafor