title: Dose-dependent effects of androgens on the circadian timing system and its response to light.
abstract: The hypothalamic suprachiasmatic nucleus (SCN) is the locus of a master clock that regulates circadian rhythms in physiology and behavior. Gonadectomy in male mice lengthens the period of circadian rhythms and increases the day-to-day variability of activity onset time. Both of these responses are rescued by the nonaromatizable androgen dihydrotestosterone. Androgen receptors (AR) are localized in SCN neurons that receive direct retinal input. To explore how androgens affect circadian clock function and its responsiveness to photic cues, we measured wheel-running behavior and SCN AR expression in intact, gonadectomized, and testosterone-replaced mice, held under various photic conditions. Gonadectomy lengthened circadian period in constant dim light but not in constant darkness. Increasing intensities of constant light parametrically increased circadian period, and this was potentiated at all intensities by gonadectomy. In contrast, gonadectomy did not alter light-induced pupil constriction, suggesting a nonretinal locus of hormone action. In hormone-replaced animals housed in constant darkness, T concentration was positively correlated with precision of activity onset and with SCN AR expression and negatively correlated with duration of activity. We infer the existence of two androgenic mechanisms: one modulates SCN responsiveness to light, and the second modulates SCN timekeeping and locomotor activity in a dose-dependent manner. Finally, the effects of androgens on period are a result of hormonal modulation of the SCN's response to photic input rather than to a change in the inherent period of oscillators in the absence of light.
authors.affiliation: Columbia University, Department of Psychology, 406 Schermerhorn Hall, 1190 Amsterdam Avenue, New York, New York 10027, USA.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Butler
authors.forename: Ilia N
authors.initials: IN
authors.lastname: Karatsoreos
authors.forename: Joseph
authors.initials: J
authors.lastname: LeSauter
authors.forename: Rae
authors.initials: R
authors.lastname: Silver
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects*; D003624:Darkness; D004305:Dose-Response Relationship, Drug; D008029:Lighting; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009919:Orchiectomy; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D000728:Androgens; D011944:Receptors, Androgen; D013739:Testosterone