title: [Study on quality control methods for different varieties of Meconopsis of Gansu].
abstract: OBJECTIVE
  To establish the quantitative determination method and TLC identification of Meconopsis and study the Vis-UV spectrum character for fast identification.
  
  
  METHODS
  Meconopsis were identified by TLC, and the content of quercetin was determined by HPLC.
  
  
  RESULTS
  The acid hydrolyze condition was HCl-80% MeOH(8:25) 90 degrees C calefaction by water for 1.5 hour. The condition of HPLC: Hypersil-Keystone-C18 (250 mm x 4.6 mm, 5 microm) column, MeOH-Water (50: 50) as mobile phase, detection wavelength at 360nm, flow rate at 1.0 mL/min, column temperature at 35 degrees C, quercetin showed a good linear relationship at a range of 0.01 - 0.12 microg, r =0.9997. The average recovery was 98.83%, and RSD was 1.69% (n = 9). The contents of quercetin in Meconopsis integrifolia and Meconopsis quintuplinervia of Gansu were 0.1135 mg/g and 0.0842 mg/g respectively.
  
  
  CONCLUSIONS
  The method is simple, accurate and specific. It can be used for the quality control and fast identification of Meconopsis.
authors.affiliation: Gansu University of Traditional Medicine, Lanzhou 730000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
authors.forename: Qin
authors.initials: Q
authors.lastname: Fan
authors.forename: Fang
authors.initials: F
authors.lastname: Cheng
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006851:Hydrochloric Acid / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D028101:Papaveraceae / Q000737:chemistry* / Q000145:classification; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D011786:Quality Control; D011794:Quercetin / Q000032:analysis*; D015203:Reproducibility of Results; D013045:Species Specificity; D014867:Water / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D014867:Water; D011794:Quercetin; D006851:Hydrochloric Acid; D000432:Methanol