title: Shoot excision has no effect on net flux of protons, ammonium or nitrate in seedling roots of a conifer and three crop species.
abstract: A high-flux region, 5mm from the root tips of seedlings of coastal Douglas-fir (Pseudotsuga menziesii), soybean (Glycine max), zucchini (Cucurbita pepo) and pea (Pisum sativum), was monitored using a microelectrode ion flux measurement system, for changes in the net fluxes of H(+), NH(4)(+) and NO(3)(-) in response to shoot removal. In all species, careful excision of the seedling shoot had no significant effect on the net fluxes of H(+), NH(4)(+) or NO(3)(-) measured 5mm from the root tip. Experiments were carried out for up to 80min after shoot removal, and no temporal interactions were noted.
authors.affiliation: Centre for Forest Biology, University of Victoria, P.O. Box 3020, STN CSC, Victoria, B.C., Canada. bhawkins@uvic.ca
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Hawkins
authors.forename: Samantha
authors.initials: S
authors.lastname: Robbins
authors.forename: Emily M
authors.initials: EM
authors.lastname: Beinhauer
mesh_terms: D001693:Biological Transport, Active; D018556:Crops, Agricultural / Q000378:metabolism; D028464:Cucurbita / Q000378:metabolism; D017136:Ion Transport; D019684:Magnoliopsida / Q000378:metabolism*; D018519:Meristem / Q000737:chemistry; D009566:Nitrates / Q000378:metabolism*; D018532:Pisum sativum / Q000502:physiology; D018517:Plant Roots / Q000378:metabolism*; D018520:Plant Shoots / Q000254:growth & development*; D011522:Protons; D028224:Pseudotsuga / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D036226:Seedlings / Q000502:physiology; D013025:Glycine max / Q000378:metabolism; D013045:Species Specificity
chemical_list: D009566:Nitrates; D011522:Protons; D000644:Quaternary Ammonium Compounds