title: Optimized serodiagnosis of Mycoplasma pneumoniae infections.
abstract: Serologic methods are well established for the diagnosis of Mycoplasma pneumoniae infections in humans, but they are less sensitive than polymerase chain reaction (PCR). To improve their sensitivity, a new panel of antigens was tested. Compared with PCR results, up to 92% of PCR-positive patients were confirmed by our immunoblotting approach having a specificity between 92.6% and 100%.
authors.affiliation: Institute of Medical Microbiology and Hygiene, Dresden University of Technology, Fetscherstrasse 74, Dresden, Germany. roger.dumke@tu-dresden.de
authors.forename: Roger
authors.initials: R
authors.lastname: Dumke
authors.forename: Andreas
authors.initials: A
authors.lastname: Strubel
authors.forename: Camille
authors.initials: C
authors.lastname: Cyncynatus
authors.forename: Hélène
authors.initials: H
authors.lastname: Nuyttens
authors.forename: Richard
authors.initials: R
authors.lastname: Herrmann
authors.forename: Christian
authors.initials: C
authors.lastname: Lück
authors.forename: Enno
authors.initials: E
authors.lastname: Jacobs
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000737:chemistry*; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007223:Infant; D008297:Male; D008875:Middle Aged; D009177:Mycoplasma pneumoniae / Q000276:immunology / Q000302:isolation & purification*; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D012137:Respiratory System / Q000382:microbiology; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D011994:Recombinant Proteins