title: Cell type-specific protein and transcription profiles implicate periarbuscular membrane synthesis as an important carbon sink in the mycorrhizal symbiosis.
abstract: The development of an arbuscular mycorrhizal (AM) symbiosis is a non-synchronous process with typical mycorrhizal root containing different symbiotic stages at one time. Methods providing cell type-specific resolution are therefore required to separate these stages and analyze each particular structure independently from each other.         We established an experimental system for analyzing specific proteomic changes in arbuscule-containing cells of Glomus intraradices colonized Medicago truncatula roots. The combination of laser capture microdissection (LCM) and liquid chromatography-tandem mass chromatography (LC-MS/MS) allowed the identification of proteins with specific or increased expression in arbuscule-containing cells. Consistent with previous transcriptome data, the proteome of arbuscule-containing cells showed an increased number of proteins involved in lipid metabolism, most likely related to the synthesis of the periarbuscular membrane. In addition, transcriptome data of non-colonized cells of mycorrhizal roots suggest mobilization of carbon resources and their symplastic transport toward arbuscule-containing cells for the synthesis of periarbuscular membranes. This highlights the periarbuscular membrane as important carbon sink in the mycorrhizal symbiosis.
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Golm, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Gaude
authors.forename: Waltraud X
authors.initials: WX
authors.lastname: Schulze
authors.forename: Philipp
authors.initials: P
authors.lastname: Franken
authors.forename: Franziska
authors.initials: F
authors.lastname: Krajinski
mesh_terms: D001692:Biological Transport / Q000235:genetics; D050260:Carbohydrate Metabolism / Q000235:genetics; D057965:Carbon Sequestration / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D055137:Glomeromycota / Q000502:physiology; D061346:Laser Capture Microdissection; D050356:Lipid Metabolism / Q000235:genetics; D046913:Medicago truncatula / Q000166:cytology / Q000235:genetics / Q000382:microbiology*; D038821:Mycorrhizae / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology* / Q000235:genetics / Q000382:microbiology; D020543:Proteome / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013559:Symbiosis / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012333:RNA, Messenger