title: Salvianolic acid A protects human SH-SY5Y neuroblastoma cells against H₂O₂-induced injury by increasing stress tolerance ability.
abstract: Salvianolic acid A (Sal A) is a polyphenol extracted from the root of the Salvia miltiorrhiza bunge. Hydrogen peroxide (H(2)O(2)) is a major reactive oxygen species (ROS), which has been implicated in stroke and other neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. In this study, we investigated the neuroprotective effects of Sal A in human SH-SY5Y neuroblastoma cells against H(2)O(2)-induced injury. Our results showed that cells pretreated with Sal A exhibited enhanced neuronal survival and that this protection was associated with an increase in adenosine triphosphate (ATP) and the stabilization of mitochondrial membrane potential. In addition, Sal A markedly decreased the excessive activation AMP-activated protein kinase (AMPK) and the serine-threonine protein kinase, Akt, in SH-SY5Ycells induced by H(2)O(2). In conclusion, our results demonstrated that Sal A protects SH-SY5Y cells against H(2)O(2)-induced oxidative stress and these protective effects are related to stress tolerance and not energy depletion via inhibition of the AMPK and Akt signaling pathway.
authors.affiliation: Beijing Key Laboratory of Drug Target Identification and Drug Screening, Chinese Academy of Medical Science and Peking Union Medical College, 1 Xian Nong Tan Street, Beijing 100050, PR China.
authors.forename: Heng-ai
authors.initials: HA
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Gao
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Li-li
authors.initials: LL
authors.lastname: Shi
authors.forename: Bai-nian
authors.initials: BN
authors.lastname: Chen
authors.forename: Yue-hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Shou-bao
authors.initials: SB
authors.lastname: Wang
authors.forename: Guan-hua
authors.initials: GH
authors.lastname: Du
mesh_terms: D000091162:AMP-Activated Protein Kinase Kinases; D002109:Caffeic Acids / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019610:Cytoprotection; D004734:Energy Metabolism / Q000187:drug effects; D006801:Humans; D007773:Lactates / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011494:Protein Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D014867:Water / Q000494:pharmacology
chemical_list: D002109:Caffeic Acids; D007773:Lactates; D018696:Neuroprotective Agents; D014867:Water; C066201:salvianolic acid A; D011494:Protein Kinases; D051057:Proto-Oncogene Proteins c-akt; D000091162:AMP-Activated Protein Kinase Kinases