title: Immunohistochemical study of osteopontin, Ki-67, and CD34 of psoriasis in Mansoura, Egypt.
abstract: OBJECTIVE
  Psoriasis is a chronic inflammatory skin disease characterized by hyper-proliferation, abnormal differentiation, and inflammatory infiltration in epidermis and dermis. We planned this study to analyze probable associations between Osteopontin (OPN), Ki-67, CD34, and histopathological features in psoriasis.
  
  
  METHODS
  We studied OPN expression and its correlation with Ki-67 and CD34 expression in lesional, non-lesional skin, and normal skin. Immunoreactivity for OPN and Ki-67 was based on the level of epidermal staining. CD34 expression was scored as mild, moderate, and strong, according to the number of stained dermal capillaries.
  
  
  RESULTS
  Our results showed statistically significant differences in the expression of OPN, Ki-67, and CD34 between lesional and non-lesional skin as well as between non-lesional skin and control group (P ≤ 0.001). In addition, there was a significant difference in the expression of OPN, Ki-67, and CD34 between control and lesional group (P=0.02, P=0.02, and P=0.04, respectively).
  
  
  CONCLUSIONS
  OPN expression seems to be related to Ki-67 (proliferation index) and CD34 expression (angiogenesis marker) confirming its role in the pathogenesis of psoriasis. Then "anti- OPN and anti-angiogenesis" may eventually become a useful therapeutic approach in psoriasis.
authors.affiliation: Department of Pathology, Mansoura Faculty of Medicine, Mansoura, Egypt. Mahaamin64@yahoo.com
authors.forename: Maha Mohamed
authors.initials: MM
authors.lastname: Amin
authors.forename: Zeinab Abdel
authors.initials: ZA
authors.lastname: Azim
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D002648:Child; D004534:Egypt; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis*; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D053495:Osteopontin / Q000032:analysis*; D011565:Psoriasis / Q000473:pathology*; D012867:Skin / Q000737:chemistry* / Q000473:pathology*; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D019394:Ki-67 Antigen; D053495:Osteopontin