title: Does the tissue engineering architecture of poly(3-hydroxybutyrate) scaffold affects cell-material interactions?
abstract: A critical element in tissue engineering involves the fabrication of a three-dimensional scaffold. The scaffold provides a space for new tissue formation, supports cellular ingrowth, and proliferation and mimics many roles of the extracellular matrix. Poly(3-hydroxybutyrate) (PHB) is the most thoroughly investigated member of the polyhydroxyalkanoates (PHAs) family that has various degrees of biocompatibility and biodegradability for tissue engineering applications. In this study, we fabricated PHB scaffolds by utilizing electrospinning and salt-leaching procedures. The behavior of monkey epithelial kidney cells (Vero) and mouse mesenchymal stem cells (mMSCs) on these scaffolds was compared by the MTS assay and scanning electron microscopy. Additionally, this study investigated the mechanical and physical properties of these scaffolds by measuring tensile strength and modulus, dynamic contact angle and porosity. According to our results, the salt-leached scaffolds showed more wettability and permeability, but inferior mechanical properties when compared with nanofibrous scaffolds. In terms of cell response, salt-leached scaffolds showed enhanced Vero cell proliferation, whereas both scaffolds responded similarly in the case of mMSCs proliferation. In brief, nanofibrous scaffolds can be a better substrate for cell attachment and morphology.
authors.affiliation: Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: Elahe
authors.initials: E
authors.lastname: Masaeli
authors.forename: Mohammad
authors.initials: M
authors.lastname: Morshed
authors.forename: Parsa
authors.initials: P
authors.lastname: Rasekhian
authors.forename: Saeed
authors.initials: S
authors.lastname: Karbasi
authors.forename: Khadije
authors.initials: K
authors.lastname: Karbalaie
authors.forename: Fereshte
authors.initials: F
authors.lastname: Karamali
authors.forename: Daryoush
authors.initials: D
authors.lastname: Abedi
authors.forename: Shahnaz
authors.initials: S
authors.lastname: Razavi
authors.forename: Abbas
authors.initials: A
authors.lastname: Jafarian-Dehkordi
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Nasr-Esfahani
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D006885:Hydroxybutyrates; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014709:Vero Cells; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates