title: Isolation and structure elucidation of antioxidant compounds from leaves of Laurus nobilis and Emex spinosus.
abstract: In recent years, there has been increasing interest in finding naturally occurring antioxidants from plants for use in food and medicinal materials to replace synthetic antioxidants since such antioxidants are being restricted due to their side effects like carcinogenicity. The aim of this work was to examine the in vitro antioxidant activity of Laurus nobilis and Emex spinosus leaves and to isolate and structurally elucidate the active compounds in those leaves. The aqueous ethanolic extracts (70%) of Laurus nobilis and Emex spinosus leaves exhibited free radical scavenging action against 1,1-diphenyl-2-picrylhydrazyl (DPPH). Their concentrations of 50% inhibition (IC(50)) were 25.3 and 20.73 μg/mL, respectively. Activity-guided separation of these extracts using a combination of different chromatographic methods (TLC and column chromatography) resulted in the isolation of five chromatographically pure compounds (three from Laurus nobilis and two from Emex spinosus leaves). Spectroscopic methods ((1)H, (13)C-NMR, UV and MS) and chemical methods (detection tests and acidic hydrolysis) revealed the isolated antioxidant compounds to be flavonoid substances that were identified as kaempferol, kaempferol-3-rhamnopyranoside, and kaempferol-3,7-dirhamnopyranoside from Laurus nobilis extract and luteolin and rutin from Emex spinosus extract. The five flavonoids had varying ability to inhibit DPPH radicals (IC(50) from 4 to 35.8 μg/mL). Luteolin and rutin had strong scavenging action with an IC(50) of 4 and 4.6 μg/mL, respectively, and this action was stronger than that of synthetic antioxidant BHA, i.e., butylated hydroxyanisole (IC50 = 5.6 μg/mL).
authors.affiliation: Biochemistry Department, Faculty of Agriculture, Fayoum University, Egypt.
authors.forename: A M
authors.initials: AM
authors.lastname: Emam
authors.forename: M A
authors.initials: MA
authors.lastname: Mohamed
authors.forename: Y M
authors.initials: YM
authors.lastname: Diab
authors.forename: N Y
authors.initials: NY
authors.lastname: Megally
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry; D027442:Laurus; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts