title: Selective apoptotic effect of Zelkova serrata twig extract on mouth epidermoid carcinoma through p53 activation.
abstract: Apoptosis or programmed cell death plays an essential role in chemotherapy-induced tumor cell killing, and inducers of apoptosis are commonly used in cancer therapy. Treatment with Zelkova serrata extracts was performed in human gingival fibroblast (HGF), mouth epidermoid carcinoma cell (KB), lower gingival squamous cancer cell (YD38) and tongue mucoepidermoid carcinoma cells (YD15). We observed that extract prepared from Zelkova serrata twig selectively inhibited proliferation of various oral cancer cells, but not normal gingival fibroblasts, in a dose-dependent manner. Caspase-8-mediated apoptosis was induced by treatment with the extract only in mouth epidermoid carcinoma and not in other types of cancer cells, including lower gingival squamous cell carcinoma. The selective apoptotic effect of Zelkova serrata twig extract in mouth epidermoid carcinoma was dependent on normal p53 status. Apoptosis was not remarkably induced by treatment with the extract in either lower gingival squamous or tongue mucoepidermoid carcinoma cells, both of which contain abnormalities of p53. Upon treatment with Zelkova serrata twig extract, mouth epidermoid carcinoma cells accumulated in S phase by activation of p21. These data indicate that Zelkova serrata twig extract exerted a cancer type-specific, p53-dependent apoptotic effect and disturbed the cell cycle, which suggests that herbal medicine could be a treatment for specific types of cancers.
authors.affiliation: Department of Nanobiomedical Science, Dankook University, Cheonan, Korea. yjjang@dankook.ac.kr
authors.forename: Hoe-Jin
authors.initials: HJ
authors.lastname: Kang
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Jang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D053148:Caspase 3 / Q000187:drug effects; D045744:Cell Line, Tumor; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006131:Growth Inhibitors / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D015398:Signal Transduction / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects*; D034401:Ulmaceae / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D006131:Growth Inhibitors; D010936:Plant Extracts; D016159:Tumor Suppressor Protein p53; D053148:Caspase 3