title: Development of topical hydrogels containing genistein-loaded nanoemulsions.
abstract: This article describes the development of topical hydrogels containing genistein-loaded nanoemulsions, obtained by means of spontaneous emulsification. This procedure yielded monodisperse nanoemulsions in a sub 250 nm range exhibiting negative zeta-potential and low viscosity. The formulations were incorporated into acrylic-acid hydrogels in order to have their viscosity adjusted for topical application. The semisolid formulations exhibit non-Newtonian pseudoplastic behavior. The skin permeation/retention of genistein from formulations was carried out using porcine ear skin mounted in Franz diffusion cells under sink conditions. The results showed a slow flow of genistein through the skin. Higher amount of genistein was detected into the skin from the formulation composed by medium chain triglycerides as oily core when compared to the octyldodecanol one. The overall results show that hydrogels containing genistein-loaded nanoemulsions could be considered as a promising formulation to delivery isoflavones into the skin.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Faculdade de Farmácia, Programa de Pós-Graduação em Ciências Farmacêuticas, Porto Alegre, RS, 90.610-000 Brazil.
authors.forename: Bethânia Andrade
authors.initials: BA
authors.lastname: de Vargas
authors.forename: Juliana
authors.initials: J
authors.lastname: Bidone
authors.forename: Laura Karsburg
authors.initials: LK
authors.lastname: Oliveira
authors.forename: Leticia Scherer
authors.initials: LS
authors.lastname: Koester
authors.forename: Valquiria Linck
authors.initials: VL
authors.lastname: Bassani
authors.forename: Helder Ferreira
authors.initials: HF
authors.lastname: Teixeira
mesh_terms: D000287:Administration, Topical; D000818:Animals; D004655:Emulsions / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D005233:Fatty Alcohols / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D019833:Genistein / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D020100:Hydrogels / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D012212:Rheology; D012867:Skin / Q000737:chemistry / Q000378:metabolism; D012869:Skin Absorption / Q000187:drug effects; D013552:Swine; D014280:Triglycerides / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D004655:Emulsions; D005233:Fatty Alcohols; D020100:Hydrogels; D014280:Triglycerides; C456972:octyl dodecanol; D019833:Genistein