title: [The inhibitory effect of luteolin on inflammation in LPS-induced microglia].
abstract: OBJECTIVE
  To study the inhibitory effect of Luteolin on LPS-induced BV-2 cell.
  
  
  METHODS
  BV-2 cells were treated with LPS (0.1 microg/mL) for inflammation model; MTT assay was used to detect the viability of BV-2 cells; Nitric oxide (NO) was detected by the method of nitric acid reductase assay; Induce type II nitric oxide synthase (iNOS) enzyme activity was determined by type of nitric oxide synthase assay;TLR4 protein expression was examined by the Western Blot analysis.
  
  
  RESULTS
  Luteolin significantly decreased the NO production and TLR4 protein expression as well as iNOS activity in LPS-activated microglial cell.
  
  
  CONCLUSIONS
  LPS induced activation of microglia lead to inflammatory response and its mechanism may be related to inhibiting TLR4 signaling pathway.
authors.affiliation: Department of Pharmacology of Traditional Chinese Medicine, College of Pharmacy, School of Medicine, Jinan University, Guangzhou 510632, China. hftxyy@yahoo.com.cn
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Zhu
authors.forename: Hua-Ming
authors.initials: HM
authors.lastname: Deng
authors.forename: Zai-Jun
authors.initials: ZJ
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiao
authors.forename: Hong
authors.initials: H
authors.lastname: Nie
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology; D047311:Luteolin / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D051197:Toll-Like Receptor 4; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D047311:Luteolin