title: [The purification and characterization of polysaccharides isolated from Ginkgo biloba and their in vitro antioxidant activities].
abstract: OBJECTIVE
  To isolate and identify the polysaccharides of Ginkgo biloba and determine their antioxidant activities in vitro.
  
  
  METHODS
  Used hot extraction and alcohol precipitation to get the crude polysaccharides, removed protein with Sevag method, further purify by column chromatography packed with DEAE-52 and DEAE-Sepharose Fast Flow. The homogeneity and molecular weights were evaluated, configuration and monosaccharide composition were measured by IR and high performance ion chromatography analysis, the scavenging activities of GBPB-S on hydroxyl radical and DPPH were measured.
  
  
  RESULTS
  The molecular weights of GBPB-W and GBPB-S were 26 300 and 19 100, and both of them were composed of rhamnose, arabinose, galactose, glucose and mannose with the ratios of (3.48: 8.47:3.73: 1.76: 1) and (5.34: 5.37: 5.27: 1:1.68). GBPB-S had certain scavenging effect on hydroxyl radical and DPPH.
  
  
  CONCLUSIONS
  The polysaccharide isolated from Ginkgo biloba has a direct antioxidant activities in vitro.
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou 510632, China. huxuqiao1988@sina.com
authors.forename: Xu-Qiao
authors.initials: XQ
authors.lastname: Hu
authors.forename: Fei
authors.initials: F
authors.lastname: Yuan
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Yan
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Huang
authors.forename: Rong-Min
authors.initials: RM
authors.lastname: Yu
mesh_terms: D001713:Biphenyl Compounds / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000378:metabolism; D008970:Molecular Weight; D009005:Monosaccharides / Q000737:chemistry; D010851:Picrates / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013057:Spectrum Analysis / Q000379:methods; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl