title: Identification of molecular compartments and genetic circuitry in the developing mammalian kidney.
abstract: Lengthy developmental programs generate cell diversity within an organotypic framework, enabling the later physiological actions of each organ system. Cell identity, cell diversity and cell function are determined by cell type-specific transcriptional programs; consequently, transcriptional regulatory factors are useful markers of emerging cellular complexity, and their expression patterns provide insights into the regulatory mechanisms at play. We performed a comprehensive genome-scale in situ expression screen of 921 transcriptional regulators in the developing mammalian urogenital system. Focusing on the kidney, analysis of regional-specific expression patterns identified novel markers and cell types associated with development and patterning of the urinary system. Furthermore, promoter analysis of synexpressed genes predicts transcriptional control mechanisms that regulate cell differentiation. The annotated informational resource (www.gudmap.org) will facilitate functional analysis of the mammalian kidney and provides useful information for the generation of novel genetic tools to manipulate emerging cell populations.
authors.affiliation: Department of Stem Cell and Regenerative Biology, Department of Molecular and Cellular Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: M Todd
authors.initials: MT
authors.lastname: Valerius
authors.forename: Mary
authors.initials: M
authors.lastname: Duah
authors.forename: Karl
authors.initials: K
authors.lastname: Staser
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Hansard
authors.forename: Jin-Jin
authors.initials: JJ
authors.lastname: Guo
authors.forename: Jill
authors.initials: J
authors.lastname: McMahon
authors.forename: Joe
authors.initials: J
authors.lastname: Vaughan
authors.forename: Diane
authors.initials: D
authors.lastname: Faria
authors.forename: Kylie
authors.initials: K
authors.lastname: Georgas
authors.forename: Bree
authors.initials: B
authors.lastname: Rumballe
authors.forename: Qun
authors.initials: Q
authors.lastname: Ren
authors.forename: A Michaela
authors.initials: AM
authors.lastname: Krautzberger
authors.forename: Jan P
authors.initials: JP
authors.lastname: Junker
authors.forename: Rathi D
authors.initials: RD
authors.lastname: Thiagarajan
authors.forename: Philip
authors.initials: P
authors.lastname: Machanick
authors.forename: Paul A
authors.initials: PA
authors.lastname: Gray
authors.forename: Alexander
authors.initials: A
authors.lastname: van Oudenaarden
authors.forename: David H
authors.initials: DH
authors.lastname: Rowitch
authors.forename: Charles D
authors.initials: CD
authors.lastname: Stiles
authors.forename: Qiufu
authors.initials: Q
authors.lastname: Ma
authors.forename: Sean M
authors.initials: SM
authors.lastname: Grimmond
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Bailey
authors.forename: Melissa H
authors.initials: MH
authors.lastname: Little
authors.forename: Andrew P
authors.initials: AP
authors.lastname: McMahon
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism; D051379:Mice; D014566:Urogenital System / Q000378:metabolism*