title: Octopamine, unlike other trace amines, inhibits responses of astroglia-enriched cultures to lipopolysaccharide via a β-adrenoreceptor-mediated mechanism.
abstract: Trace amines (TAs), i.e. β-phenylethylamine, tyramine and octopamine, are generally regarded as sympathomimetic compounds with structural and functional analogy with catecholamines. Previous reports have shown particularly high levels of circulating TAs in migraine and cluster headache patients. However, no clues are yet available as to the pathophysiological significance of these alterations. The effect of different TAs on the release of nitric oxide was investigated in rat astroglial cells stimulated with lipopolysaccharide (LPS). Octopamine substantially inhibited the release of NO evoked by LPS. Tyramine and β-PEA were ineffective. The inhibitory effect of octopamine was fully reverted by two selective antagonists of β-adrenergic receptors, while α-adrenergic blockade was ineffective. These data, consistent with a role of octopamine as a modulator of NO release, uncover an interaction between octopamine and β-adrenergic receptors in astroglial cells. These results may have an impact in understanding the mechanisms underlying migraine pathophysiology.
authors.affiliation: Research & Innovation S.p.A., Via Svizzera 16, 35127 Padova, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: D'Andrea
authors.forename: Antonello
authors.initials: A
authors.lastname: D'Arrigo
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Facchinetti
authors.forename: Elda
authors.initials: E
authors.lastname: Del Giudice
authors.forename: Davide
authors.initials: D
authors.lastname: Colavito
authors.forename: Daniele
authors.initials: D
authors.lastname: Bernardini
authors.forename: Alberta
authors.initials: A
authors.lastname: Leon
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D009655:Octopamine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018342:Receptors, Adrenergic, beta-1 / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D014439:Tyramine / Q000494:pharmacology; D000095383:Trace Amine-Associated Receptors
chemical_list: D008070:Lipopolysaccharides; D009569:Nitric Oxide; D009655:Octopamine; D018342:Receptors, Adrenergic, beta-1; D043562:Receptors, G-Protein-Coupled; D014439:Tyramine; D000095383:Trace Amine-Associated Receptors