title: Molecular typing of dengue virus circulating in kolkata, India in 2010.
abstract: Dengue is one of the major public health threats in Kolkata. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Kolkata for the detection of dengue infection in them. In 2010, a total of 378 samples were referred to us for that purpose. All the samples were tested for the detection of IgM antibodies by ELISA method, followed by RT-PCR test for the detection of serotypes. Only 173 samples were ELISA positive. Out of 378 samples, 108 were RT-PCR positive. Out of 108 samples, 74 samples had monotypic infection with different serotypes of DENV and 33 samples had dual infections with DENV-2 and DENV-3. Only one sample had the infection with DENV-1, DENV-2, and DENV-3. DHF was found mainly among the patients, infected with multiple dengue serotypes. Only 3 dengue monotypic infected patients had suffered from DHF.
authors.affiliation: ICMR Virus Unit, Kolkata, ID & BG Hospital Campus 57, Dr. S. C. Banerjee Road, Beliaghata, Kolkata 700010, India.
authors.forename: Arindam
authors.initials: A
authors.lastname: Sarkar
authors.forename: Debjani
authors.initials: D
authors.lastname: Taraphdar
authors.forename: Shyamalendu
authors.initials: S
authors.lastname: Chatterjee