title: Overexpression of DNA polymerase iota (Polι) in esophageal squamous cell carcinoma.
abstract: The present study investigated the transcriptional regulation of low-fidelity translesion DNA synthesis (TLS) polymerases in human esophageal carcinoma. Significantly higher mRNA expression of polymerase zeta (Polξ), RAD18, polymerase iota (Polι), and polymerase kappa (Polκ) was found in esophageal carcinomas. The increased expression of Polι in tumor samples was further confirmed by immunohistochemistry. The promoter of POLI that encodes Polι was found to be hypomethylated, although the overexpression of this gene was unlikely to be associated with methylation in tumors. We further identified Sp1 and Oct-1 binding sites present in the POLI promoter. We observed that the binding affinity of Sp1 to the POLI promoter was significantly increased in cancerous tissues and that Sp1 activated POLI gene transcription in cultured cell lines. The present study demonstrates overexpression of the TLS genes in esophageal carcinoma and identifies a key role for Sp1 in upregulating POLI gene expression.
authors.affiliation: School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China.
authors.forename: Jundong
authors.initials: J
authors.lastname: Zhou
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Xie
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Xie
authors.forename: Jinchang
authors.initials: J
authors.lastname: Wu
authors.forename: Jianping
authors.initials: J
authors.lastname: Cao
authors.forename: Wei-Qun
authors.initials: WQ
authors.lastname: Ding
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D019175:DNA Methylation; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism*; D004947:Esophagus / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D000094769:DNA Polymerase iota
chemical_list: D012333:RNA, Messenger; D004259:DNA-Directed DNA Polymerase; D000094769:DNA Polymerase iota; C000722070:POLI protein, human