title: Effect of agitation and aeration on the citric acid production by Yarrowia lipolytica grown on glycerol.
abstract: The effects of agitation rates from 400 to 900 rpm and aeration rates ranging from 0.18 to 0.6 vvm on biomass and citric acid production on glycerol media by acetate-negative mutants of Yarrowia lipolytica, Wratislavia 1.31 and Wratislavia AWG7, in batch culture were studied. The agitation rates of 800 and 900 rpm (at a constant aeration rate of 0.36 vvm) and aeration rates within the range of 0.24-0.48 vvm (at a constant agitation rate of 800 rpm), which generated dissolved oxygen concentration (DO) higher than 40%, were found the best for citric acid biosynthesis from glycerol. An increase in agitation rate (higher than 800 rpm) and aeration rate (higher than 0.36 vvm) had no impact on DO and citric acid production. The highest citric acid concentration (92.8 g/L) and yield (0.63 g/g) were obtained with Wratislavia 1.31 strain at 0.24 vvm. The highest volumetric citric acid production rate (1.15 g/Lh) and specific citric acid production rate (0.071 g/gh) were reached at 0.48 vvm.
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland. Anita.Rywinska@up.wroc.pl
authors.forename: Anita
authors.initials: A
authors.lastname: Rywińska
authors.forename: Izabela
authors.initials: I
authors.lastname: Musiał
authors.forename: Waldemar
authors.initials: W
authors.lastname: Rymowicz
authors.forename: Barbara
authors.initials: B
authors.lastname: Zarowska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Boruczkowski
mesh_terms: D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D019343:Citric Acid / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D005990:Glycerol / Q000737:chemistry*; D007218:Industrial Microbiology; D007523:Isocitrates / Q000737:chemistry; D025282:Mycelium / Q000737:chemistry / Q000254:growth & development; D010100:Oxygen / Q000737:chemistry; D025062:Yarrowia / Q000737:chemistry* / Q000254:growth & development
chemical_list: D003470:Culture Media; D007523:Isocitrates; D019343:Citric Acid; C034219:isocitric acid; D005990:Glycerol; D010100:Oxygen