title: [Two new sequence type isolates of Bacillus anthracis by multilocus sequence typing].
abstract: OBJECTIVE
  To define the sequence type (ST) isolates of Bacillus anthracis by multilocus sequence typing (MLST).
  
  
  METHODS
  Fragments of seven housekeeping genes (glpF, gmk, ilvD, pta, pur, pycA, and tpi) were amplified by PCR using the standard primers as described on the website for MLST of Bacillus and the sequences were compared with existing allele sequences on the MLST website.
  
  
  RESULTS
  Two novel allele combinations of the seven loci were found in two isolates 17003-14 and 17003-32.
  
  
  CONCLUSIONS
  Two novel ST isolates of B. anthracis were identified by this study and confirmed by the MLST website, and the pubMLST ids were id-1053 and id-1054.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. zuott0307@gmail.com
authors.forename: Tingting
authors.initials: T
authors.lastname: Zuo
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Li
authors.forename: Xuelian
authors.initials: X
authors.lastname: Han
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.forename: Qing
authors.initials: Q
authors.lastname: Duan
mesh_terms: D001408:Bacillus anthracis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D058885:Multilocus Sequence Typing / Q000379:methods*; D016133:Polymerase Chain Reaction