title: Process parameters study of α-amylase production in a packed-bed bioreactor under solid-state fermentation with possibility of temperature monitoring.
abstract: Production of α-amylase in a laboratory-scale packed-bed bioreactor by Bacillus sp. KR-8104 under solid-state fermentation (SSF) with possibility of temperature control and monitoring was studied using wheat bran (WB) as a solid substrate. The simultaneous effects of aeration rate, initial substrate moisture, and incubation temperature on α-amylase production were evaluated using response surface methodology (RSM) based on a Box-Behnken design. The optimum conditions for attaining the maximum production of α-amylase were 37°C, 72% (w/w) initial substrate moisture, and 0.15 L/min aeration. The average enzyme activity obtained under the optimized conditions was 473.8 U/g dry fermented substrate. In addition, it was observed that the production of enzyme decreased from the bottom of the bioreactor to the top.
authors.affiliation: Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, Tehran, Iran.
authors.forename: Sorour
authors.initials: S
authors.lastname: Derakhti
authors.forename: Seyed Abbas
authors.initials: SA
authors.lastname: Shojaosadati
authors.forename: Maryam
authors.initials: M
authors.lastname: Hashemi
authors.forename: Khosro
authors.initials: K
authors.lastname: Khajeh
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D003470:Culture Media / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism; D004789:Enzyme Activation; D057075:Enzyme Assays; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D013696:Temperature; D000516:alpha-Amylases / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D004043:Dietary Fiber; D000516:alpha-Amylases