title: Geobacter luticola sp. nov., an Fe(III)-reducing bacterium isolated from lotus field mud.
abstract: A novel species of Fe(III)-reducing bacterium, designated strain OSK6(T), belonging to the genus Geobacter, was isolated from lotus field mud in Japan. Strain OSK6(T) was isolated using a solid medium containing acetate, Fe(III)-nitrilotriacetate (NTA) and gellan gum. The isolate is a strictly anaerobic, gram-negative, motile, straight rod-shaped bacterium, 0.6-1.9 µm long and 0.2-0.4 µm wide. The growth of the isolate occurred at 20-40 °C with optima of 30-37 °C and pH 6.5-7.5 in the presence of up to 0.5 g NaCl l(-1). The G+C content of the genomic DNA was determined by HPLC to be 59.7 mol%. The major respiratory quinone was MK-8. The major fatty acids were 16 : 1ω7c and 16 : 0. Strain OSK6(T) was able to grow with Fe(III)-NTA, ferric citrate, amorphous iron (III) hydroxide and nitrate, but not with fumarate, malate or sulfate as electron acceptors. Among examined substrates grown with Fe(III)-NTA, the isolate grew on acetate, lactate, pyruvate and succinate. Analysis of the near full-length 16S rRNA gene sequence revealed that strain OSK6(T) is closely related to Geobacter daltonii and Geobacter toluenoxydans with 95.6 % similarity to the type strains of these species. On the basis of phylogenetic analysis and physiological tests, strain OSK6(T) is described as a representative of a novel species, Geobacter luticola sp. nov.; the type strain is OSK6(T) ( = DSM 24905(T) = JCM 17780(T)).
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido 501-1193, Gifu, Japan.
authors.forename: Samson
authors.initials: S
authors.lastname: Viulu
authors.affiliation: Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido 501-1193, Gifu, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido 501-1193, Gifu, Japan.
authors.forename: Yurina
authors.initials: Y
authors.lastname: Okada
authors.affiliation: Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido 501-1193, Gifu, Japan.
authors.forename: Sakiko
authors.initials: S
authors.lastname: Saitou
authors.affiliation: Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido 501-1193, Gifu, Japan.|United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido 501-1193, Gifu, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takamizawa
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005290:Ferric Compounds / Q000378:metabolism*; D045302:Geobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D000070116:Lotus / Q000382:microbiology*; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D005290:Ferric Compounds; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8