title: Interaction between pheromone and its receptor of the fission yeast Schizosaccharomyces pombe examined by a force spectroscopy study.
abstract: Interaction between P-factor, a peptide pheromone composed of 23 amino acid residues, and its pheromone receptor, Mam2, on the cell surface of the fission yeast Schizosaccharomyces pombe was examined by an atomic force microscope (AFM). An AFM tip was modified with P-factor derivatives to perform force curve measurements. The specific interaction force between P-factor and Mam2 was calculated to be around 120 pN at a probe speed of 1.74 μm/s. When the AFM tip was modified with truncated P-factor derivative lacking C-terminal Leu, the specific interaction between the tip and the cell surface was not observed. These results were also confirmed with an assay system using a green fluorescent protein (GFP) reporter gene to monitor the activation level of signal transduction following the interaction of Mam2 with P-factor.
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Kanagawa, Yokohama, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Sasuga
authors.forename: Ryohei
authors.initials: R
authors.lastname: Abe
authors.forename: Osamu
authors.initials: O
authors.lastname: Nikaido
authors.forename: Shoichi
authors.initials: S
authors.lastname: Kiyosaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sekiguchi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ikai
authors.forename: Toshiya
authors.initials: T
authors.lastname: Osada
mesh_terms: D049452:Green Fluorescent Proteins / Q000032:analysis / Q000378:metabolism; D018625:Microscopy, Atomic Force; D010675:Pheromones / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D043563:Receptors, Pheromone / Q000737:chemistry / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010675:Pheromones; D043563:Receptors, Pheromone; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C085847:map2 protein, S pombe; D049452:Green Fluorescent Proteins