title: Highly sensitive multiple microRNA detection based on fluorescence quenching of graphene oxide and isothermal strand-displacement polymerase reaction.
abstract: A simple, highly sensitive, and selective multiple microRNA (miRNA) detection method based on the graphene oxide (GO) fluorescence quenching and isothermal strand-displacement polymerase reaction (ISDPR) was proposed. The capability to discriminate ssDNA and double-stranded nucleic acid structure coupled with the extraordinary fluorescence quenching of GO on multiple organic dye allows the proposed strategy to simultaneously and selectively detect several miRNA labeled with different dyes in the same solution, while the ISDPR amplification endows the detection method with high sensitivity. The strong interaction between ssDNA and GO led to the fluorescent ssDNA probe exhibiting minimal background fluorescence. Upon the recognition of specific target miRNA, an ISDPR was triggered to produce numerous massive specific DNA-miRNA duplex helixes, and a strong emission was observed due to the weak interaction between the DNA-miRNA duplex helix and GO. A miRNA biosensor down to 2.1 fM with a linear range of 4 orders of magnitude was obtained. Furthermore, the large planar surface of GO allows simultaneous quenching of several DNA probes with different dyes and produces a multiple biosensing platform with high sensitivity and selectivity, which has promising application in profiling the pattern of miRNA expression and biomedical research.
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing, PR China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Dong
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
authors.forename: Huiting
authors.initials: H
authors.lastname: Lu
authors.forename: Shiyan
authors.initials: S
authors.lastname: Wang
authors.forename: Shi
authors.initials: S
authors.lastname: Jin
authors.forename: Kaihong
authors.initials: K
authors.lastname: Hao
authors.forename: Hongwu
authors.initials: H
authors.lastname: Du
authors.forename: Xueji
authors.initials: X
authors.lastname: Zhang
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D010087:Oxides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D035683:MicroRNAs; D010087:Oxides; D006108:Graphite