title: Minipig model of maxillary distraction osteogenesis: immunohistochemical and histomorphometric analysis of the sequence of osteogenesis.
abstract: OBJECTIVE
  To document the sequence of bone formation in a minipig model of Le Fort I distraction osteogenesis (DO) using immunohistochemistry and histomorphometry.
  
  
  METHODS
  Female Yucatan minipigs (N = 9) in the mixed-dentition stage underwent bilateral maxillary DO. The distraction protocol was 0 days of latency, with a distraction rate of 1 mm/d for 12 days and 24 days of fixation. Specimens were harvested and divided between the central incisors (18 hemi-maxillae) at the end of DO (n = 6), at mid-fixation (n = 6), and at the end of fixation (n = 6). Sections, including the advancement zone, were stained with hematoxylin-eosin, collagen II, CD34, proliferating cell nuclear antigen, and tartrate-resistant acid phosphatase. Light and fluorescence microscope images (original magnification ×200) were obtained, and percentage of surface area (PSA) of the advancement zone occupied by fibrous tissue, vessels, proliferating cells, osteoid, and bone was determined. An intact maxilla served as the control.
  
  
  RESULTS
  At the end of DO, in the advancement zone, the PSA (mean values) of proliferating cells was 33.16%; fibrous tissue, 52%; vessels, 4.35%; and new bone, 5.45%. At the end of fixation, the PSA of proliferating cells decreased to 10.53%, fibrous tissue to 2.3%, and vessels to 1.5% whereas the PSA of new bone increased to 44.9%.
  
  
  CONCLUSIONS
  The results of this study indicate that the progression of osteogenesis in the maxillary DO wound begins with intense cellular proliferation and vascular fibrous tissue formation and progresses to mature, cancellous bone by the end of fixation. The PSA occupied by mature bone is significantly less than in the control maxilla at the end of fixation. This is consistent with the sequence in the mandibular DO wound.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA 02114, USA. mpapadaki@partners.org
authors.forename: Maria E
authors.initials: ME
authors.lastname: Papadaki
authors.forename: Leonard B
authors.initials: LB
authors.lastname: Kaban
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Troulis
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D049109:Cell Proliferation; D024043:Collagen Type II / Q000032:analysis; D005260:Female; D007124:Immunoenzyme Techniques; D008437:Maxilla / Q000601:surgery*; D023421:Models, Animal; D010006:Osteoblasts / Q000737:chemistry; D010012:Osteogenesis / Q000502:physiology*; D019857:Osteogenesis, Distraction; D019340:Osteotomy, Le Fort / Q000379:methods*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D013552:Swine; D013556:Swine, Miniature
chemical_list: D018952:Antigens, CD34; D024043:Collagen Type II; D018809:Proliferating Cell Nuclear Antigen