title: Regeneration of comparative genomic hybridization oligonucleotide microarrays with dimethylurea.
abstract: Reuse of materials in DNA hybridization-based methods has been known since the advent of Southern membranes. Array-based comparative genomic hybridization is essentially Southern hybridization with multiple probes immobilized on a solid surface. We show that comparative genomic hybridization microarrays fabricated with maskless array synthesizer technology can be used up to four times with the application of 1,3-dimethylurea as an array-stripping agent. We reproducibly detected chromosomal aberrations (0.6-22.4Mb in size) in four hybridization rounds using regenerated microarray slides. We also demonstrated that regenerated arrays can detect smaller alterations (16-200kbp), such as common copy number variants, as well as complex aberration profiles in tumor DNA.
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, 80-416 Gdansk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Ronowicz
authors.forename: Magdalena
authors.initials: M
authors.lastname: Brzeskwiniewicz
authors.forename: Piotr
authors.initials: P
authors.lastname: Madanecki
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Buckley
authors.forename: Ewa
authors.initials: E
authors.lastname: Orłowska
authors.forename: J
authors.initials: J
authors.lastname: Renata Ochocka
authors.forename: Janusz
authors.initials: J
authors.lastname: Limon
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Piotrowski
mesh_terms: D055028:Comparative Genomic Hybridization; D004247:DNA / Q000378:metabolism; D056915:DNA Copy Number Variations; D006801:Humans; D008782:Methylurea Compounds / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D008782:Methylurea Compounds; D004247:DNA; C018276:1,3-dimethylurea