title: In vitro and in vivo studies on the antioxidant activity of fish peptide isolated from the croaker (Otolithes ruber) muscle protein hydrolysate.
abstract: Peptide from croaker (Otolithes ruber) muscle protein hydrolysate was purified, characterized and evaluated for its in vitro and in vivo antioxidant activity. Results showed that purified peptide contained the amino acid sequence as Lys-Thr-Phe-Cys-Gly-Arg-His (861.6Da), which were expected to contribute to its antioxidant activities. This peptide efficiently quenched 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals (84.5±1.2 and 62.4±2.9%), and successfully inhibits the lipid peroxidation and DNA damage and proven to be a potent antioxidant at different in vitro systems. It also improved the endogenous cellular antioxidant enzymes in Wistar rat by increasing the activities of catalase (CAT), glutathione-S-transferase (GST) and superoxide dismutase (SOD) after supplementation of the peptide (283.6±7.25, 4.3±0.78 and 28.42±1.97) compared to the negative control (196.4±5.65, 1.3±0.45 and 15.1±0.35). Therefore, croaker muscle peptide can increase an endurance capacity and facilitate recovery from oxidative stress.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, Tamilnadu, India. nazeer.ra@ktr.srmuniv.ac.in
authors.forename: R A
authors.initials: RA
authors.lastname: Nazeer
authors.forename: N S Sampath
authors.initials: NS
authors.lastname: Kumar
authors.forename: R
authors.initials: R
authors.lastname: Jai Ganesh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D029941:Fish Proteins / Q000737:chemistry / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D017665:Hydroxyl Radical / Q000737:chemistry / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010473:Perciformes / Q000378:metabolism*; D010851:Picrates / Q000737:chemistry / Q000378:metabolism; D011492:Protein Hydrolysates / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D029941:Fish Proteins; D009124:Muscle Proteins; D010851:Picrates; D011492:Protein Hydrolysates; D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase