title: [Measurement of the amino acid sequence for the fusion protein FP3 with LC-MS/MS].
abstract: The amino acid sequence of the fusion protein FP3 was measured by two types of LC-MS/MS and its primary structure was confirmed. After reduction and alkylation, the protein was digested with trypsin and glycosyl groups in glycopeptide were removed by PNGase F. The mixed peptides were separated by LC, then Q-TOF and Ion trap tandem mass spectrometry were used to measure b, y fragment ions of each peptide to analyze the amino acid sequence of fusion protein FP3. Seventy-six percent of full amino acid sequence of the fusion protein FP3 was measured by LC-ESI-Q-TOF with the remaining 24% completed by LC-ESI-Trap. As LC-MS and tandem mass spectrometry are rapid, sensitive, accurate to measure the protein amino acid sequence, they are important approach to structure analysis and identification of recombinant protein.
authors.affiliation: Department of Recombinant Product, National Institutes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing 100050, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Tao
authors.forename: De-Ning
authors.initials: DN
authors.lastname: Pei
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Rao
authors.forename: Jun-Zhi
authors.initials: JZ
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors* / Q000737:chemistry
chemical_list: C557495:FP3 VEGF receptor Fc-fusion protein; D011993:Recombinant Fusion Proteins; D042461:Vascular Endothelial Growth Factor A