title: Human Gyrovirus Apoptin shows a similar subcellular distribution pattern and apoptosis induction as the chicken anaemia virus derived VP3/Apoptin.
abstract: The chicken anaemia virus-derived protein Apoptin/VP3 (CAV-Apoptin) has the important ability to induce tumour-selective apoptosis in a variety of human cancer cells. Recently the first human Gyrovirus (HGyV) was isolated from a human skin swab. It shows significant structural and organisational resemblance to CAV and encodes a homologue of CAV-Apoptin/VP3. Using overlapping primers we constructed a synthetic human Gyrovirus Apoptin (HGyV-Apoptin) fused to green fluorescent protein in order to compare its apoptotic function in various human cancer cell lines to CAV-Apoptin. HGyV-Apoptin displayed a similar subcellular expression pattern as observed for CAV-Apoptin, marked by translocation to the nucleus of cancer cells, although it is predominantly located in the cytosol of normal human cells. Furthermore, expression of either HGyV-Apoptin or CAV-Apoptin in several cancer cell lines triggered apoptosis at comparable levels. These findings indicate a potential anti-cancer role for HGyV-Apoptin.
authors.affiliation: Head and Neck Oncology Group, Department of Oral Pathology, King's College London Dental Institute, Guy's Hospital Campus, London SE1 9RT, UK.
authors.forename: J
authors.initials: J
authors.lastname: Bullenkamp
authors.forename: D
authors.initials: D
authors.lastname: Cole
authors.forename: F
authors.initials: F
authors.lastname: Malik
authors.forename: H
authors.initials: H
authors.lastname: Alkhatabi
authors.forename: A
authors.initials: A
authors.lastname: Kulasekararaj
authors.forename: E W
authors.initials: EW
authors.lastname: Odell
authors.forename: F
authors.initials: F
authors.lastname: Farzaneh
authors.forename: J
authors.initials: J
authors.lastname: Gäken
authors.forename: M
authors.initials: M
authors.lastname: Tavassoli
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D002477:Cells / Q000821:virology*; D017727:Chicken anemia virus / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D046810:Gyrovirus / Q000378:metabolism*; D045325:HCT116 Cells; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D051017:Apoptosis Regulatory Proteins; D036022:Capsid Proteins; D011993:Recombinant Fusion Proteins; C091717:VP3 protein, Chicken anemia virus; D049452:Green Fluorescent Proteins