title: Expression of adhesion and activation molecules on circulating monocytes in children with Helicobacter pylori infection.
abstract: OBJECTIVE
  The aim of this study was to assess the cell surface expression of adhesion (CD11a, CD11b, CD11c, CD18, CD54, and CD58) and activation (CD14, HLA-DR, and CD16) molecules on the circulating monocytes in Helicobacter pylori (H. pylori)-infected and noninfected children with gastritis, with the goal of comparing the results with those obtained from the controls.
  
  
  METHODS
  Ninety-four children were studied: 47 of them with H. pylori infection (of those 25 children after the failure of eradication therapy) and 26 children with gastritis where H. pylori infection was excluded, as well as 21 controls. H. pylori infection status was assessed based on [¹³C] urea breath test, rapid urease test, and histology. Analysis of the monocyte surface molecule expression was carried out by flow cytometry.
  
  
  RESULTS
  H. pylori-infected children and children who experienced a failure of the eradication therapy differed significantly in the expression of adhesion and activation molecule on circulating monocytes. A decrease, both in the proportion of CD11c- and CD14-bearing monocytes, and the expression of CD11c and CD14 molecules on circulating monocytes, was found in children in whom the eradication therapy failed (p < .05). Low expression of CD11b (p = .04) and CD18 (p = .02) integrins on monocytes was also observed. Additionally, the percentage of HLA-DR-bearing monocytes was decreased (p = .04), while the CD16 density receptor was increased (p = .02). Compared with the controls, low percentage of CD16-positive monocytes was noted in noninfected children with gastritis (p = .01).
  
  
  CONCLUSIONS
  H. pylori eradication therapy in children causes inhibition of inflammatory response via a reduction in CD11b, CD11c, and CD18 beta2 integrin monocyte expression.
authors.affiliation: Department of Immunology, Collegium Medicum Nicolaus Copernicus University, M. Sklodowskiej-Curie 9, Bydgoszcz 85-094, Poland. a.helminbasa@gmail.com
authors.forename: Anna
authors.initials: A
authors.lastname: Helmin-Basa
authors.forename: Mieczyslawa
authors.initials: M
authors.lastname: Czerwionka-Szaflarska
authors.forename: Grazyna
authors.initials: G
authors.lastname: Bala
authors.forename: Anna
authors.initials: A
authors.lastname: Szaflarska-Poplawska
authors.forename: Grazyna
authors.initials: G
authors.lastname: Mierzwa
authors.forename: Lidia
authors.initials: L
authors.lastname: Gackowska
authors.forename: Izabela
authors.initials: I
authors.lastname: Kubiszewska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Eljaszewicz
authors.forename: Andrzej
authors.initials: A
authors.lastname: Marszalek
authors.forename: Jacek
authors.initials: J
authors.lastname: Michalkiewicz
mesh_terms: D000293:Adolescent; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D002648:Child; D005260:Female; D015870:Gene Expression; D016481:Helicobacter Infections / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008297:Male; D009000:Monocytes / Q000276:immunology*
chemical_list: D015815:Cell Adhesion Molecules