title: A V(L) single-domain antibody library shows a high-propensity to yield non-aggregating binders.
abstract: A synthetic human V(L) phage display library, created by the randomization of all complementarity-determining regions (CDRs) in a V(L) scaffold, was panned against three test antigens to determine the propensity of the library to yield non-aggregating binders. A total of 22 binders were isolated against the test antigens and the majority (20) were monomeric. Thus, human V(L) repertoires provide an efficient source of non-aggregating binders and represent an attractive alternative to human V(H) repertoires, which are notorious for containing high proportions of aggregating species. Moreover, the solubility of V(L)s, in contrast to V(H)s, appears much less CDR dependent.
authors.affiliation: Institute for Biological Sciences,  National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario,  Canada K1A 0R6.
authors.forename: Greg
authors.initials: G
authors.lastname: Hussack
authors.forename: Artine
authors.initials: A
authors.lastname: Keklikian
authors.forename: Jawaher
authors.initials: J
authors.lastname: Alsughayyir
authors.forename: Pejman
authors.initials: P
authors.lastname: Hanifi-Moghaddam
authors.forename: Mehdi
authors.initials: M
authors.lastname: Arbabi-Ghahroudi
authors.forename: Henk
authors.initials: H
authors.lastname: van Faassen
authors.forename: Sheng T
authors.initials: ST
authors.lastname: Hou
authors.forename: Subash
authors.initials: S
authors.lastname: Sad
authors.forename: Roger
authors.initials: R
authors.lastname: MacKenzie
authors.forename: Jamshid
authors.initials: J
authors.lastname: Tanha
mesh_terms: D000595:Amino Acid Sequence; D000915:Antibody Affinity; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D019151:Peptide Library; D011485:Protein Binding; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D007147:Immunoglobulin Light Chains; D019151:Peptide Library; D057127:Single-Chain Antibodies; C031815:toxB protein, Clostridium difficile