title: In vitro susceptibility of Pythium insidiosum isolates to aminoglycoside antibiotics and tigecycline.
abstract: This study evaluated the in vitro activity of aminoglycoside antibiotics and tigecycline against Pythium insidiosum. The susceptibility tests were carried out using the broth microdilution method in accordance with the CLSI document M38-A2. MIC values for gentamicin, neomycin, paromomycin, and streptomycin ranged from 32 to 64 mg/liter, and the minimal fungicidal concentration (MFC) ranged from 32 to 128 mg/liter, which are incompatible with safe concentrations of these drugs in plasma in vivo. Tigecycline showed the lowest MIC (0.25 to 2 mg/liter) and MFC (1 to 8 mg/liter) range values. The in vitro susceptibility observed to tigecycline makes this drug a good option in future tests in vitro and in vivo for the management of pythiosis.
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Deise Luiza
authors.initials: DL
authors.lastname: Mahl
authors.forename: Francielli Pantella Kunz
authors.initials: FP
authors.lastname: de Jesus
authors.forename: Érico  Silva
authors.initials: É
authors.lastname: Loreto
authors.forename: Régis Adriel
authors.initials: RA
authors.lastname: Zanette
authors.forename: Laerte
authors.initials: L
authors.lastname: Ferreiro
authors.forename: Maiara Ben
authors.initials: MB
authors.lastname: Pilotto
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
authors.forename: Janio Morais
authors.initials: JM
authors.lastname: Santurio
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000031:analogs & derivatives* / Q000494:pharmacology; D011775:Pythium / Q000187:drug effects*; D000078304:Tigecycline
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000078304:Tigecycline; D008911:Minocycline