title: Generation and administration of HA-1-specific T-cell lines for the treatment of patients with relapsed leukemia after allogeneic stem cell transplantation: a pilot study.
abstract: Since HA-1-specific T cells have been shown to make a significant contribution to the clinical responses in patients with relapsed leukemia, we investigated the feasibility of adoptive transfer of in vitro induced HA-1-specific CD8 positive T cells to patients with relapsed leukemia after allogeneic stem cell transplantation. The in vitro generation of clinical grade HA-1-specific T-cell lines from HA-1 negative donors was seen to be feasible and 3 patients were treated with HA-1-specific T-cell lines. No toxicity after infusion was observed. Although in one patient, during a period of stable disease, HA-1-specific T cells could be detected in the peripheral blood and bone marrow, these patients had no clear clinical response.
authors.affiliation: Department of Clinical Pharmacy and Toxicology, L0-P, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands. p.meij@lumc.nl
authors.forename: Pauline
authors.initials: P
authors.lastname: Meij
authors.forename: Inge
authors.initials: I
authors.lastname: Jedema
authors.forename: Menno A W G
authors.initials: MA
authors.lastname: van der Hoorn
authors.forename: Rian
authors.initials: R
authors.lastname: Bongaerts
authors.forename: Linda
authors.initials: L
authors.lastname: Cox
authors.forename: Amon R
authors.initials: AR
authors.lastname: Wafelman
authors.forename: Erik W A
authors.initials: EW
authors.lastname: Marijt
authors.forename: Roel
authors.initials: R
authors.lastname: Willemze
authors.forename: J H Frederik
authors.initials: JH
authors.lastname: Falkenburg
mesh_terms: D019264:Adoptive Transfer; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007938:Leukemia / Q000276:immunology* / Q000628:therapy*; D015778:Minor Histocompatibility Antigens / Q000276:immunology*; D009842:Oligopeptides / Q000276:immunology*; D010865:Pilot Projects; D012008:Recurrence; D014184:Transplantation, Homologous; D016896:Treatment Outcome
chemical_list: C110615:HA-1 antigen; D015778:Minor Histocompatibility Antigens; D009842:Oligopeptides