title: Single-wavelength two-photon excitation-stimulated emission depletion (SW2PE-STED) superresolution imaging.
abstract: We developed a new class of two-photon excitation-stimulated emission depletion (2PE-STED) optical microscope. In this work, we show the opportunity to perform superresolved fluorescence imaging, exciting and stimulating the emission of a fluorophore by means of a single wavelength. We show that a widely used red-emitting fluorophore, ATTO647N, can be two-photon excited at a wavelength allowing both 2PE and STED using the very same laser source. This fact opens the possibility to perform 2PE microscopy at four to five times STED-improved resolution, while exploiting the intrinsic advantages of nonlinear excitation.
authors.affiliation: Department of Nanophysics, Istituto Italiano di Tecnologia, 16100 Genoa, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Bianchini
authors.forename: Benjamin
authors.initials: B
authors.lastname: Harke
authors.forename: Silvia
authors.initials: S
authors.lastname: Galiani
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Vicidomini
authors.forename: Alberto
authors.initials: A
authors.lastname: Diaspro