title: Efficient transfection of MG-63 osteoblasts using magnetic nanoparticles and oscillating magnetic fields.
abstract: OBJECTIVE
  To examine the potential of magnetic nanoparticles (MNPs) in transfecting human osteosarcoma fibroblasts (MG-63) and investigate the effects of a novel non-viral oscillating nanomagnetic gene transfection system (magnefect-nano™) in enhancing transfection efficiency (TE).
  
  
  METHODS
  MG-63 cells were transfected using MNPs coupled with a GFP-carrying plasmid. The magnefect-nano system was evaluated for transfection efficiency and potential associated effects on cell viability.
  
  
  RESULTS
  MG-63 cells were efficiently transfected using MNPs and the magnefect-nano system significantly enhanced overall transfection efficiency. MNPs were not found to affect cell viability and/or function of the cells.
  
  
  CONCLUSIONS
  Non-viral transfection using MNPs and the magnefect-nano system can be used to transfect MG-63 cells and assist reporter gene delivery on a single cell basis, highlighting the wide potential of nanomagnetic gene transfection in gene therapy.
authors.affiliation: Institute for Science & Technology in Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent, UK.
authors.forename: A
authors.initials: A
authors.lastname: Fouriki
authors.forename: M A
authors.initials: MA
authors.lastname: Clements
authors.forename: N
authors.initials: N
authors.lastname: Farrow
authors.forename: J
authors.initials: J
authors.lastname: Dobson
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D004247:DNA / Q000737:chemistry; D018274:Electroporation; D011992:Endosomes / Q000737:chemistry; D005434:Flow Cytometry; D015316:Genetic Therapy; D049452:Green Fluorescent Proteins; D006801:Humans; D008055:Lipids / Q000737:chemistry; D060526:Magnetic Fields; D008280:Magnetics; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D009991:Oscillometry; D010006:Osteoblasts / Q000166:cytology*; D014162:Transfection / Q000379:methods*
chemical_list: D008055:Lipids; D049452:Green Fluorescent Proteins; D004247:DNA