title: Development of channeled nanofibrous scaffolds for oriented tissue engineering.
abstract: A tissue-engineering scaffold resembling the structure of the natural extracellular matrix can often facilitate tissue regeneration. Nerve and tendon are oriented micro-scale tissue bundles. In this study, a method combining injection molding and thermally induced phase separation techniques is developed to create single- and multiple-channeled nanofibrous poly(L-lactic acid) scaffolds. The overall shape, the number and spatial arrangement of channels, the channel wall matrix architecture, the porosity and mechanical properties of the scaffolds are all tunable. The porous NF channel wall matrix provides an excellent microenvironment for protein adsorption and the attachment of PC12 neuronal cells and tendon fibroblast cells, showing potential for neural and tendon tissue regeneration.
authors.affiliation: Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Chenghui
authors.initials: C
authors.lastname: Sun
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Jin
authors.forename: Jeremy M
authors.initials: JM
authors.lastname: Holzwarth
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Liu
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.forename: Melanie J
authors.initials: MJ
authors.lastname: Gupte
authors.forename: Yaoming
authors.initials: Y
authors.lastname: Zhao
authors.forename: Peter X
authors.initials: PX
authors.lastname: Ma
mesh_terms: D000818:Animals; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009416:Nerve Regeneration; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D016716:PC12 Cells; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits; D051381:Rats; D013710:Tendons / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)