title: A new approach to bioanalysis: aqueous normal-phase chromatography with silica hydride stationary phases.
abstract: Stationary phases based on silica hydride have demonstrated a number of unique properties that are especially advantageous for bioanalyses. They have excellent retention capabilities for hydrophilic compounds, which have been the most difficult to analyze by standard reversed-phase methods and, in many cases, can outperform newer approaches for the analysis of polar molecules, such as hydrophilic liquid interaction chromatography. In addition, all columns utilizing silica-hydride materials can be used in either the normal-phase or reversed-phase modes, sometimes retaining both polar and nonpolar compounds simultaneously. These stationary phases have a high degree of reproducibility and long-term stability.
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA. joseph.pesek@sjsu.edu
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Pesek
authors.forename: Maria T
authors.initials: MT
authors.lastname: Matyska
mesh_terms: D002845:Chromatography / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D010455:Peptides / Q000302:isolation & purification; D017640:Silicates / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D010455:Peptides; D017640:Silicates; C487012:silica hydride; D014867:Water