title: Characterization of potato plants with reduced StSYR1 expression.
abstract: Vesicle fusion processes in plants are important for both development and stress responses. Transgenic potato plants with reduced expression of SYNTAXIN-RELATED1 (StSYR1), a gene encoding the potato homolog of Arabidopsis PENETRATION1 (AtPEN1), display spontaneous necrosis and chlorosis at later stages of development. In accordance with this developmental defect, tuber number, weight and overall yield are significantly reduced in StSYR1-RNAi lines. Enhanced resistance of StSYR1-RNAi plants to Phytophthora infestans, the causal agent of late blight disease of potato, correlates with enhanced levels of salicylic acid, whereas levels of 12-oxophytodienoic acid and jasmonic acid are unaltered. Cultured cells of StSYR1-RNAi lines secrete at least two compounds which are not detectable in the supernatant of control cells, suggesting an involvement of StSYR1 in secretion processes to the apoplast.
authors.affiliation: Department Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany.
authors.forename: Lennart
authors.initials: L
authors.lastname: Eschen-Lippold
authors.forename: Tilo
authors.initials: T
authors.lastname: Lübken
authors.forename: Ulrike
authors.initials: U
authors.lastname: Smolka
authors.forename: Sabine
authors.initials: S
authors.lastname: Rosahl
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant; D054883:Oxylipins / Q000378:metabolism; D055750:Phytophthora infestans; D063245:Plant Development / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D035281:Plant Tubers / Q000254:growth & development* / Q000502:physiology; D030821:Plants, Genetically Modified; D050765:Qa-SNARE Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D020156:Salicylic Acid / Q000378:metabolism; D011198:Solanum tuberosum / Q000502:physiology*
chemical_list: D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D054883:Oxylipins; D010940:Plant Proteins; D050765:Qa-SNARE Proteins; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; D020156:Salicylic Acid