title: [The effects and mechanisms of BTBD10 on the proliferation of islet beta cell].
abstract: OBJECTIVE
  To explore the role of BTBD10 overexpression in the proliferation of insulinoma cell line INS-1 and its mechanism.
  
  
  METHODS
  The recombined expression plasmid of pcDNA4.0-BTBD10 was constructed by gene cloning technique and was transfected into INS-1 cell by lipofectamine 2000. The stable overexpression BTBD10 of INS-1 cell was selected at 48(th) hour after transfection. INS-1 cell proliferation activity was measured by MTT method. The expression of BTBD10, protein kinase B (Akt), phospho-Akt (p-Akt), mammal target of rapamycin (mTOR) and phospho-mTOR (p-mTOR) were determined by Western blot.
  
  
  RESULTS
  The stable overexpression BTBD10 of INS-1 cell was successfully constructed. Overproduction of BTBD10 promoted beta cell proliferation. The phosphorylation of Akt and mTOR was increased and the ratio of p-Akt/Akt and p-mTOR/mTOR was enhanced in the INS-1 overexpressed by BTBD10. But the expression of total Akt and mTOR presented no obvious changes.
  
  
  CONCLUSIONS
  The overexpression BTBD10 of INS-1 cell could activate of Akt/mTOR signalling pathway via stimulating phospho-mTOR and Akt, and enhance overall cell protein translation, so as to promote proliferation of INS-1 cell.
authors.affiliation: Department of Geriatric Endocrinology, General Hospital of PLA, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhao-yan
authors.initials: ZY
authors.lastname: Gu
authors.forename: Xin-yu
authors.initials: XY
authors.lastname: Miao
authors.forename: Yan-ping
authors.initials: YP
authors.lastname: Gong
authors.forename: Yu-jun
authors.initials: YJ
authors.lastname: Xiao
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Tian
authors.forename: Chun-lin
authors.initials: CL
authors.lastname: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D047908:Intracellular Signaling Peptides and Proteins; D007515:Islets of Langerhans / Q000166:cytology*; D009687:Nuclear Proteins / Q000235:genetics*; D010766:Phosphorylation; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D014162:Transfection
chemical_list: C494432:BTBD10 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D058570:TOR Serine-Threonine Kinases