title: Transcriptome and full-length cDNA resources for the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major insect pest of pine forests.
abstract: Bark beetles (Coleoptera: Curculionidae: Scolytinae) are major insect pests of many woody plants around the world. The mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins, is a significant historical pest of western North American pine forests. It is currently devastating pine forests in western North America--particularly in British Columbia, Canada--and is beginning to expand its host range eastward into the Canadian boreal forest, which extends to the Atlantic coast of North America. Limited genomic resources are available for this and other bark beetle pests, restricting the use of genomics-based information to help monitor, predict, and manage the spread of these insects. To overcome these limitations, we generated comprehensive transcriptome resources from fourteen full-length enriched cDNA libraries through paired-end Sanger sequencing of 100,000 cDNA clones, and single-end Roche 454 pyrosequencing of three of these cDNA libraries. Hybrid de novo assembly of the 3.4 million sequences resulted in 20,571 isotigs in 14,410 isogroups and 246,848 singletons. In addition, over 2300 non-redundant full-length cDNA clones putatively containing complete open reading frames, including 47 cytochrome P450s, were sequenced fully to high quality. This first large-scale genomics resource for bark beetles provides the relevant sequence information for gene discovery; functional and population genomics; comparative analyses; and for future efforts to annotate the MPB genome. These resources permit the study of this beetle at the molecular level and will inform research in other Dendroctonus spp. and more generally in the Curculionidae and other Coleoptera.
authors.affiliation: Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, Canada V6T 1Z4. ckeeling@alumni.sfu.ca
authors.forename: Christopher I
authors.initials: CI
authors.lastname: Keeling
authors.forename: Hannah
authors.initials: H
authors.lastname: Henderson
authors.forename: Maria
authors.initials: M
authors.lastname: Li
authors.forename: Mack
authors.initials: M
authors.lastname: Yuen
authors.forename: Erin L
authors.initials: EL
authors.lastname: Clark
authors.forename: Jordie D
authors.initials: JD
authors.lastname: Fraser
authors.forename: Dezene P W
authors.initials: DP
authors.lastname: Huber
authors.forename: Nancy Y
authors.initials: NY
authors.lastname: Liao
authors.forename: T Roderick
authors.initials: TR
authors.lastname: Docking
authors.forename: Inanc
authors.initials: I
authors.lastname: Birol
authors.forename: Simon K
authors.initials: SK
authors.lastname: Chan
authors.forename: Greg A
authors.initials: GA
authors.lastname: Taylor
authors.forename: Diana
authors.initials: D
authors.lastname: Palmquist
authors.forename: Steven J M
authors.initials: SJ
authors.lastname: Jones
authors.forename: Joerg
authors.initials: J
authors.lastname: Bohlmann
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism; D001517:Coleoptera / Q000235:genetics* / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005216:Fat Body / Q000378:metabolism; D005260:Female; D008297:Male; D005810:Multigene Family; D016366:Open Reading Frames; D028223:Pinus / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D003577:Cytochrome P-450 Enzyme System