title: Effect of postactivation treatment with latrunculin A on in vitro and in vivo development of cloned embryos derived from kidney fibroblasts of an aged Clawn miniature boar.
abstract: The objective of this study was to examine the effect of postactivation treatment with latrunculin A (LatA), an actin polymerization inhibitor, on in vitro and in vivo development of somatic cell nuclear transfer (SCNT) embryos derived from kidney fibroblasts of an aged Clawn miniature boar (12 years old). After electric activation, SCNT embryos were treated with 0, 0.5 or 1 μM LatA and cultured in vitro. The rate of blastocyst formation was significantly higher (P<0.05) in SCNT embryos treated with 0.5 μM LatA (38%) than those in control (14%). When cloned embryos treated with 0.5 μM LatA were transferred into the oviducts of two recipient miniature gilts to assess their development in vivo, both recipients became pregnant; one maintained pregnancy to term, and a live piglet (weighing 220 g) was delivered by Caesarean section. The results of this study indicated that the postactivation treatment with LatA was effective in improving in vitro developmental capacity of SCNT miniature pig embryos derived from kidney fibroblasts of an aged animal and that miniature pig cloned embryos treated with LatA had the ability to develop to term.
authors.affiliation: Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Himaki
authors.forename: Yamato
authors.initials: Y
authors.lastname: Mizobe
authors.forename: Kenichirou
authors.initials: K
authors.lastname: Tsuda
authors.forename: Masashi
authors.initials: M
authors.lastname: Suetomo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamakuchi
authors.forename: Kazuchika
authors.initials: K
authors.lastname: Miyoshi
authors.forename: Sonshin
authors.initials: S
authors.lastname: Takao
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Yoshida
mesh_terms: D051339:Actin Depolymerizing Factors / Q000494:pharmacology*; D000375:Aging; D000818:Animals; D017849:Animals, Inbred Strains; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D004477:Ectogenesis / Q000187:drug effects*; D004558:Electric Stimulation; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D047109:Fetal Development / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology; D007564:Japan; D007672:Kidney Cortex / Q000166:cytology*; D050498:Live Birth / Q000662:veterinary; D008297:Male; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D009994:Osmolar Concentration; D011247:Pregnancy; D013552:Swine; D013556:Swine, Miniature / Q000196:embryology*; D053778:Thiazolidines / Q000494:pharmacology*
chemical_list: D051339:Actin Depolymerizing Factors; D019086:Bridged Bicyclo Compounds, Heterocyclic; D053778:Thiazolidines; C037067:latrunculin A