title: [Proteomics analysis of distinct proteins in human atherosclerosis obliterans: identification and verification].
abstract: OBJECTIVE
  To identify distinct proteins involved in human atherosclerosis obliterans (ASO) by a differential proteomic approach.
  
  
  METHODS
  Eight atherosclerotic femoral arteries with a mean age of 68.6 years (6 male and 2 female) and 5 normal femoral arteries with a mean age of 44.2 years (3 male and 2 female) were obtained from high amputation patients. Then the first 2-dimensional maps of the proteome of human femoral arteries was plotted to compare ASO and control specimens. Proteomic profiling was to differentiate and identify histological proteins that were associated with ASO. The differentially expressed proteins were sequenced by matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS). The result was verified by immunohistochemistry (IHC) and Western blot.
  
  
  RESULTS
  ASO was associated with distinct patterns of protein expression in the femoral arteries. A total of 25 distinct spots corresponding to 13 different proteins were identified by MALDI-TOF-MS using the NCBI and IPI databases. These proteins were mainly involved in the pathogenetic mechanisms such as inflammation, oxidative stress, proliferation and transformation of SMCs. The low level of heat shock protein 27 (HSP27) in ASO was verified by IHC and western-blot in accord with the result of MS.
  
  
  CONCLUSIONS
  Proteomic analysis can be used to investigate differentially expressed proteins, which may provide new insights into ASO pathogenesis, such as HSP27.
authors.affiliation: Department of Vascular Surgery, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Vascular Center of Shanghai Jiaotong University, Shanghai 200011, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhao
authors.forename: Hai-guang
authors.initials: HG
authors.lastname: Zhao
authors.forename: Guang
authors.initials: G
authors.lastname: Liu
authors.forename: Xin-wu
authors.initials: XW
authors.lastname: Lu
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Mi-er
authors.initials: ME
authors.lastname: Jiang
mesh_terms: D000328:Adult; D000368:Aged; D001162:Arteriosclerosis Obliterans / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000378:metabolism*
chemical_list: D020543:Proteome