title: Generating aptamers by cell-SELEX for applications in molecular medicine.
abstract: Aptamers are single-stranded oligonucleotides of DNA or RNA that bind to target molecules with high affinity and specificity. Typically, aptamers are generated by an iterative selection process, called systematic evolution of ligands by exponential enrichment (SELEX). Recent advancements in SELEX technology have extended aptamer selection from comparatively simple mixtures of purified proteins to whole living cells, and now cell-based SELEX (or cell-SELEX) can isolate aptamers that bind to specific target cells. Combined with nanotechnology, microchips, microfluidic devices, RNAi and other advanced technologies, cell-SELEX represents an integrated platform providing ultrasensitive and highly specific tools for clinical medicine. In this review, we describe the recent progress made in the application of cell-SELEX for diagnosis, therapy and biomarker discovery.
authors.affiliation: State Key Laboratory of Chem/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha 410082, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Ye
authors.affiliation: Hunan Provincial Tumor Hospital, Changsha 410013, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Xiangya School of Medicine , Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Minyuan
authors.initials: M
authors.lastname: Peng
authors.affiliation: Xiangya School of Medicine , Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Xiangya School of Medicine , Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Xiangya School of Medicine , Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Huixia
authors.initials: H
authors.lastname: Liu
authors.affiliation: Xiangya School of Medicine , Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Xielan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Chem/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha 410082, China.|Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000627:therapeutic use; D045744:Cell Line, Tumor; D006801:Humans; D008024:Ligands; D046210:Microfluidic Analytical Techniques; D058990:Molecular Targeted Therapy; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D008024:Ligands