title: Establishment of a new cell line for performing sensitive screening of nuclear export inhibitors.
abstract: Cell nuclear import and export of proteins are among the most important processes for cellular homeostasis. Viruses, including influenza virus and HIV-1 also utilize nuclear export machinery for their propagation. Therefore, the specific inhibition of nuclear export can be a good target for the development of antiviral drugs. This report documents the establishment of Madin-Darby Canine Kidney (MDCK) cells stably expressing GFP-NES fusion protein for the sensitive screening of novel nuclear export inhibitors. Leptomycin B (LMB), an inhibitor for the interaction between substrate nuclear export signal (NES) and cellular export factor, CRM1, was used for evaluating the screening system. The low concentration (30 pg/mL) and short time treatment (~10 min) of LMB resulted in the nuclear accumulation of GFP fluorescence.
authors.affiliation: Laboratory of Molecular Biology of Infectious Agents, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Noda
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi