title: Alkyl hydroperoxide reductase: a candidate Helicobacter pylori vaccine.
abstract: Helicobacter pylori (H. pylori) is the most important etiological agent of chronic active gastritis, peptic ulcer disease and gastric cancer. The aim of this study was to evaluate the efficacy of alkyl hydroperoxide reductase (AhpC) and mannosylated AhpC (mAhpC) as candidate vaccines in the C57BL/6J mouse model of H. pylori infection. Recombinant AhpC was cloned, over-expressed and purified in an unmodified form and was also engineered to incorporate N and C-terminal mannose residues when expressed in the yeast Pichia pastoris. Mice were immunized systemically and mucosally with AhpC and systemically with mAhpC prior to challenge with H. pylori. Serum IgG responses to AhpC were determined and quantitative culture was used to determine the efficacy of vaccination strategies. Systemic prophylactic immunization with AhpC/alum and mAhpC/alum conferred protection against infection in 55% and 77.3% of mice, respectively. Mucosal immunization with AhpC/cholera toxin did not protect against infection and elicited low levels of serum IgG in comparison with systemic immunization. These data support the use of AhpC as a potential vaccine candidate against H. pylori infection.
authors.affiliation: Department of Clinical Medicine and Institute of Molecular Medicine, Trinity College Dublin, Ireland.
authors.forename: Avril A
authors.initials: AA
authors.lastname: O'Riordan
authors.forename: Veronica Athie
authors.initials: VA
authors.lastname: Morales
authors.forename: Linda
authors.initials: L
authors.lastname: Mulligan
authors.forename: Nazia
authors.initials: N
authors.lastname: Faheem
authors.forename: Henry J
authors.initials: HJ
authors.lastname: Windle
authors.forename: Dermot P
authors.initials: DP
authors.lastname: Kelleher
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000534:Alum Compounds / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D006023:Glycoproteins / Q000276:immunology; D016481:Helicobacter Infections / Q000276:immunology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000201:enzymology* / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D054464:Peroxiredoxins / Q000276:immunology*; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D006023:Glycoproteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; C041524:aluminum sulfate; D054464:Peroxiredoxins