title: Quantification of glucose, xylose, arabinose, furfural, and HMF in corncob hydrolysate by HPLC-PDA-ELSD.
abstract: Lignocellulose and other carbohydrates are being studied extensively as potential renewable carbon sources for liquid biofuels and other valuable chemicals. In the present study, a simple, sensitive, selective, and reliable HPLC method using a photodiode array (PDA) detector and an evaporative light scattering detector (ELSD) was developed for the simultaneous determination of important sugars (D(+)-cellobiose, glucose, xylose, and arabinose), furfural and 5-hydroxymethylfurfural (5-HMF) in lignocellulose hydrolysate. The analysis was carried out on an Aminex HPX-87H column (250 mm × 4.6 mm, 5 μm particle size). Ultra-pure water with 0.00035 M H(2)SO(4) was used as the mobile phase with a flow rate of 0.6 mL/min. The temperature of the ELSD drift tube was kept at 50 °C, the carrier gas pressure was 350 kPa, and the gain was set at 7. Furfural and 5-HMF were quantified on a PDA detector at 275 nm and 284 nm, respectively. The sugar concentrations were determined by ELSD. This method was validated for accuracy and precision. The regression equation revealed a good linear relationship (r(2) = 0.9986 ± 0.0012) within the test ranges. The method showed good reproducibility for the quantification of six analytes in corncob hydrolysate, with intra- and inter-day variations less than 1.12%. This method is also convenient because it allows the rapid analysis of the primary products of biomass hydrolysis and carbohydrate degradation.
authors.affiliation: Zhejiang Province Key Laboratory of Biofuel, College of Chemical Engineering and Materials Science, Xipeilou 207, Zhejiang University of Technology, Chaowang Road 18#, Xiacheng District, Hangzhou, Zhejiang 310014, China. liuxuejun@zjut.edu.cn
authors.forename: Xuejun
authors.initials: X
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Ai
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Meizhen
authors.initials: M
authors.lastname: Lu
authors.forename: Dengxiang
authors.initials: D
authors.lastname: Ji
authors.forename: Fengwen
authors.initials: F
authors.lastname: Yu
authors.forename: Jianbing
authors.initials: J
authors.lastname: Ji
mesh_terms: D001089:Arabinose / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis*; D005947:Glucose / Q000032:analysis*; D014994:Xylose / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
chemical_list: C008046:5-hydroxymethylfurfural; D014994:Xylose; D001089:Arabinose; D005662:Furaldehyde; D005947:Glucose