title: [Study on tissue culture and plant regeneration of the stem-tips and buds of Periploca forrestii].
abstract: OBJECTIVE
  To establish the rapid propagation systems of the stem-tips and buds of Periploca forrestii.
  
  
  METHODS
  Inserted the stem-tips and buds of Periploca forrestii into MS medium with different concentrations of 6-BA, NAA and 2.4-D and induced them growing into complete plants.
  
  
  CONCLUSIONS
  The optimal culture medium for bud induction is MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L and the bud induction rate can reach 86.29%. The optimal culture medium for stem-tips induction is MS +6-BA 2.0 mg/L + NAA 0.5 mg/L and the bud induction rate can reach 86.29%. The optimal culture medium for bud multiplication is MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L and the multiplication coefficient can reach 2.10. The best rooting medium is 1/2 MS + IBA 0.5 mg/L and the rooting rate is 53.33%.
authors.affiliation: Agriculture And Biotechnology School, Southwest University, Chongqing 400716, China. happylzf1234@126.com
authors.forename: Zhi-Fei
authors.initials: ZF
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Niu
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Tao-Tao
authors.initials: TT
authors.lastname: Chen
mesh_terms: D003470:Culture Media; D031109:Periploca / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D012038:Regeneration; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators