title: High-performance liquid chromatography-mass spectrometry and evaporative light-scattering detector to compare phenolic profiles of muscadine grapes.
abstract: Evaporative light scattering detector (ELSD) is considered as a universal detector able to detect almost any compound. This study successfully established a HPLC-ELSD method for the simultaneous determination of phenolic acids, flavonoids and anthocyanins in muscadine grape. Twenty-four phenolics, including 5 anthocyanins, were identified by HPLC-UV-MS, ten of them were selected to calibrate the ELSD. Results from the ELSD quantification suggested that gallic acid, proanthocyanidins, and ellagic acid were the main phenolics in muscadines. In addition, antioxidant tests suggested that the extracts of muscadine grapes possessed strong antioxidant activities. All extracts had a high total phenolic content (TPC). High total anthocyanins contents (TAC) were found in Noble muscadine, and high proanthocyanidins content (TPA) in the seed portion.
authors.affiliation: Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, SC 29634, USA.
authors.forename: Qi
authors.initials: Q
authors.lastname: You
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Julia L
authors.initials: JL
authors.lastname: Sharp
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.forename: Yuru
authors.initials: Y
authors.lastname: You
authors.forename: Chuanji
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D005707:Gallic Acid / Q000032:analysis; D008027:Light; D013058:Mass Spectrometry / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D012044:Regression Analysis; D012542:Scattering, Radiation; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D005707:Gallic Acid