title: [Study on fingerprint of semen cassiae, ultramicro powder and powder particle].
abstract: OBJECTIVE
  To establish HPLC fingerprint of Semen Cassiae.
  
  
  METHODS
  Analysis on a Phenomenex C18 (4.6 mm x 250 mm, 5 microm) column eluted with phases containing acetonitrile and 0.1% phosphoric acid in water in gradient mode. The flow rate was 0.8 mL/min and the detection wavelength was 280 nm. The temperature of column was 30 degrees C. Data of 12 batches of Semen Cassiae, ultramicro powder and powder particle from different sources were analysed by "similarity evaluation for chromatographic Fingerprint of Traditional Chinese Medicine" software, and the similarity of them was compared.
  
  
  RESULTS
  Twenty-seven common peaks were identified in the chromatograms,and all the common peaks were separated effectively. Fingerprint of ultramicro powder and Semen Cassiae was basic similar, but fingerprint of powder particle and Semen Cassiae was obviously different.
  
  
  CONCLUSIONS
  The method is precise, repeatabe and stable. It can be used to identity and evaluate the quality of Semen Cassiae, and provide important theoretical foundation for clinical applications of Semen Cassiae.
authors.affiliation: Chemistry and Chemical Engineering Department of Huaihua University, Huaihua 418008, China. tangyulian0824@163.com
authors.forename: Yu-Lian
authors.initials: YL
authors.lastname: Tang
authors.forename: Yi-Zeng
authors.initials: YZ
authors.lastname: Liang
authors.forename: Hua-Xiu
authors.initials: HX
authors.lastname: Zhang
authors.forename: Ji-Yin
authors.initials: JY
authors.lastname: Xu
mesh_terms: D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D010316:Particle Size; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D011208:Powders