title: Effects of reactive nitrogen scavengers on NK-cell-mediated killing of K562 cells.
abstract: This study explored the effects of reactive nitrogen metabolites (RNMS) on natural-killer- (NK-) cell-mediated killing of K562 cells and the influence of RNM scavengers, such as tiopronin (TIP), glutamylcysteinylglycine (GSH), and histamine dihydrochloride (DHT), on reversing the suppressing effect of RNM. We administered exogenous and endogenous RNM in the NK + K562 culture system and then added RNM scavengers. The concentrations of RNM, TNF-β and IFN-γ, and NK-cell cytotoxicity (NCC) and the percentage of living NK cells were then examined. We found that both exogenous and endogenous RNM caused the KIR to decrease (P < 0.01); however, RNM scavengers such as TIP and GSH rescued this phenomenon dose dependently. In conclusion, our data suggests that RNM scavengers such as TIP and GSH enhance the antineoplasmic activity of NK cells.
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Fujian, Quanzhou 362000, China.
authors.forename: Yili
authors.initials: Y
authors.lastname: Zeng
authors.forename: Qinmiao
authors.initials: Q
authors.lastname: Huang
authors.forename: Meizhu
authors.initials: M
authors.lastname: Zheng
authors.forename: Jianxin
authors.initials: J
authors.lastname: Guo
authors.forename: Jingxin
authors.initials: J
authors.lastname: Pan
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D030421:Peroxynitrous Acid / Q000378:metabolism; D008625:Tiopronin / Q000494:pharmacology
chemical_list: D016207:Cytokines; D016166:Free Radical Scavengers; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; D006632:Histamine; D008625:Tiopronin; D005978:Glutathione