title: [Expression of transforming growth factor beta 1 and conection tissue growth factor in ankylosing spondylitis].
abstract: OBJECTIVE
  To investigate the expressions of transforming growth factor beta 1(TGF-β1) /smad, connective tissue growth factor (CTGF), collagen I and collagen III in ankylosing spondylitis (AS).
  
  
  METHODS
  Thirty patients with AS were included in the study. All the patients were performed with computed tomography-guided needle biopsy in sacroiiliac joint. Sera TGF-β1 and CTGF were determined by enzyme-linked immunosorbent assay (ELISA).  Immunohistologic studies were performed with the alkaline phosphatase-anti-alkaline phosphatase technique to assess the expressions of TGF-β1, p-smad3, smad7, CTGF, collagen I and collagen III in sacroiiliac joint tissue samples.
  
  
  RESULTS
  In the AS patients, neither serum TGF-β1 level nor serum CTGF level was found significantly different from that of the controls [(6.7±2.1)mg/L vs.(5.4±5.8)mg/L, P<0.05, (0.83±0.46)μg/L vs.(1.07±0.79 )μg/L, P<0.05]. In contrast to the healthy controls, TGF-β1 and CTGF were found upexpressed in cytoplasm of inflammatory cells in pannus and bone  marrow in sacroiliac tissue samples of patients with AS [(104.5±66.2) /HP vs. (24.4±9.3) /HP, (57.94±42.40) /HP vs. (2.67±2.52) /HP]. Meantime, p-smad3  was found expressed in the nuclear, while smad7 was detected to be downexpressed. Additionally, collagen I and collagen III were found upexpressed in  bone, cartilage and ligament tissue.
  
  
  CONCLUSIONS
  TGF-β1, CTGF, collagen I and collagen III were upexpressed in sarcoiliac joints of AS patients. TGF-β1/CTGF may play an important role in articular cartilage fibrosis and ossification of AS by smad signal pathyway.
authors.affiliation: Department of Rheumatology and Immunology, Peking University Shenzhen Hospital, Shenzhen, 518000, China. wqw_sw@163.com
authors.forename: Qing-wen
authors.initials: QW
authors.lastname: Wang
authors.forename: Pei-ying
authors.initials: PY
authors.lastname: Zeng
authors.forename: Yue-ming
authors.initials: YM
authors.lastname: Cai
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Lu
authors.forename: Hui Yao
authors.initials: HY
authors.lastname: Lan
mesh_terms: D000328:Adult; D002358:Cartilage, Articular / Q000473:pathology; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000378:metabolism*; D005260:Female; D005355:Fibrosis / Q000209:etiology; D006801:Humans; D008297:Male; D015398:Signal Transduction; D051785:Smad Proteins / Q000378:metabolism; D013167:Spondylitis, Ankylosing / Q000378:metabolism* / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D055815:Young Adult
chemical_list: C526317:CCN2 protein, human; D024042:Collagen Type I; D024061:Collagen Type III; D051785:Smad Proteins; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor