title: Dynamics of Setdb1 expression in early mouse development.
abstract: Setdb1/Eset, a histone lysine methyltransferase, is recruited by various transcription factors to modify local chromatin. The observation that Setdb1-null blastocysts fail to produce epiblast-lineage cells suggests a role for Setdb1 in generating mouse embryonic stem cells (mESCs). When examined in mouse zygotes, Setdb1 proteins appeared as dots at perinucleolar rims of pronuclei, with the dot-shaped signals more prominent in male pronuclei. Setdb1 signals were observed diffusely in the nucleus from the two-cell stage onward and, by the blastocyst, took a punctate form, away from nucleolus. Such varying expression patterns suggest its involvement in diverse biological processes at preimplantation stage. Setdb1 appeared in Oct4-positive cells of inner-cell-mass origin but not in trophectoderm-lineage cells in blastocyst outgrowths. Setdb1 co-immunoprecipitated with Oct4 in mESCs, and Setdb1 expression was markedly reduced upon retinoic acid-induced differentiation. These observations suggest that Setdb1 has an important role in maintaining the self-renewal of mESCs through collaboration with Oct4.
authors.affiliation: Development and Differentiation Research Center, KRIBB, 111 Gwahangno, Yuseong-gu, Daejeon 305-806, South Korea. swcho@kribb.re.kr
authors.forename: Sunwha
authors.initials: S
authors.lastname: Cho
authors.forename: Jung Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Sujin
authors.initials: S
authors.lastname: Kwon
authors.forename: Yong-Kook
authors.initials: YK
authors.lastname: Kang
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000737:chemistry / Q000378:metabolism; D002454:Cell Differentiation; D002466:Cell Nucleolus / Q000378:metabolism; D004622:Embryo, Mammalian / Q000737:chemistry / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000737:chemistry / Q000378:metabolism; D011495:Histone-Lysine N-Methyltransferase; D008297:Male; D051379:Mice; D011496:Protein Methyltransferases / Q000032:analysis / Q000235:genetics*; D014212:Tretinoin / Q000378:metabolism; D015053:Zygote / Q000737:chemistry / Q000166:cytology / Q000378:metabolism
chemical_list: D014212:Tretinoin; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; C516239:SETDB1 protein, mouse