title: Interfacial electronic effects in functional biolayers integrated into organic field-effect transistors.
abstract: Biosystems integration into an organic field-effect transistor (OFET) structure is achieved by spin coating phospholipid or protein layers between the gate dielectric and the organic semiconductor. An architecture directly interfacing supported biological layers to the OFET channel is proposed and, strikingly, both the electronic properties and the biointerlayer functionality are fully retained. The platform bench tests involved OFETs integrating phospholipids and bacteriorhodopsin exposed to 1-5% anesthetic doses that reveal drug-induced changes in the lipid membrane. This result challenges the current anesthetic action model relying on the so far provided evidence that doses much higher than clinically relevant ones (2.4%) do not alter lipid bilayers' structure significantly. Furthermore, a streptavidin embedding OFET shows label-free biotin electronic detection at 10 parts-per-trillion concentration level, reaching state-of-the-art fluorescent assay performances. These examples show how the proposed bioelectronic platform, besides resulting in extremely performing biosensors, can open insights into biologically relevant phenomena involving membrane weak interfacial modifications.
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Bari A. Moro, 70126 Bari, Italy.
authors.forename: Maria Daniela
authors.initials: MD
authors.lastname: Angione
authors.forename: Serafina
authors.initials: S
authors.lastname: Cotrone
authors.forename: Maria
authors.initials: M
authors.lastname: Magliulo
authors.forename: Antonia
authors.initials: A
authors.lastname: Mallardi
authors.forename: Davide
authors.initials: D
authors.lastname: Altamura
authors.forename: Cinzia
authors.initials: C
authors.lastname: Giannini
authors.forename: Nicola
authors.initials: N
authors.lastname: Cioffi
authors.forename: Luigia
authors.initials: L
authors.lastname: Sabbatini
authors.forename: Emiliano
authors.initials: E
authors.lastname: Fratini
authors.forename: Piero
authors.initials: P
authors.lastname: Baglioni
authors.forename: Gaetano
authors.initials: G
authors.lastname: Scamarcio
authors.forename: Gerardo
authors.initials: G
authors.lastname: Palazzo
authors.forename: Luisa
authors.initials: L
authors.lastname: Torsi