title: Simple and specific detection of Bordetella holmesii by using a loop-mediated isothermal amplification assay.
abstract: A loop-mediated isothermal amplification (LAMP) assay for simple detection of Bordetella holmesii was developed. This assay discriminates between B. holmesii and other Bordetella species and successfully detect B. holmesii DNA in nasopharyngeal swab samples from subjects with suspected pertussis. The LAMP assay results were in complete agreement with the results of previously published real-time PCR assay, indicating that the former is a powerful tool for the accurate diagnosis and surveillance of B. holmesii.
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Otsuka
authors.forename: Shuji
authors.initials: S
authors.lastname: Yoshino
authors.forename: Kimiko
authors.initials: K
authors.lastname: Kawano
authors.forename: Hiromi
authors.initials: H
authors.lastname: Toyoizumi-Ajisaka
authors.forename: Keigo
authors.initials: K
authors.lastname: Shibayama
authors.forename: Kazunari
authors.initials: K
authors.lastname: Kamachi
mesh_terms: D001884:Bordetella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D014917:Whooping Cough / Q000175:diagnosis* / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial