title: Discrepancies in identification of Vibrio cholerae strains as members of Aeromonadaceae and Enterobacteriaceae by automated microbial identification system.
abstract: OBJECTIVE
  Incidental observation of a discrepancy in identification of Vibrio cholerae prompted a study to understand the ability of an automated microbial identification system to identify this important pathogen.
  
  
  RESULTS
  Twenty clinical isolates of V. cholerae showing difference in genetic profiles by random amplified polymorphic DNA (RAPD) fingerprinting, serologically confirmed as O1, and showing presence of ctxA and tcpA genes in PCR were subjected to analysis by Vitek 2 Compact automated identification system for identification. Vitek 2 Compact detected 10 of 20 isolates correctly, whereas the remaining 10 were identified as various members of Aeromonadaceae and Enterobacteriaceae.
  
  
  CONCLUSIONS
  Our results indicate that Vitek 2 Compact automated microbial system does not always identify V. cholerae strains correctly.
  
  
  CONCLUSIONS
  These observations should create awareness among end users about possible misidentifications by automated systems and encourage simultaneous use of serology and/or PCR for correct identification at least for V. cholerae, which is one of the most important enteric pathogens.
authors.affiliation: Department of Health Research, Regional Medical Research Centre, Indian Council of Medical Research, Belgaum, Karnataka, India.
authors.forename: A
authors.initials: A
authors.lastname: Saini
authors.forename: H
authors.initials: H
authors.lastname: Kaur
authors.forename: S
authors.initials: S
authors.lastname: Purwar
authors.forename: S D
authors.initials: SD
authors.lastname: Kholkute
authors.forename: S
authors.initials: S
authors.lastname: Roy
mesh_terms: D044044:Aeromonadaceae / Q000145:classification / Q000235:genetics; D057205:Automation, Laboratory; D001431:Bacteriological Techniques; D004755:Enterobacteriaceae / Q000145:classification / Q000235:genetics; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D014734:Vibrio cholerae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification