title: An electrochemical method to detect gamma glutamyl transpeptidase.
abstract: Gamma glutamyl transpeptidase (GGT) is a transferase, which is of great importance in sustaining intracellular cysteine and glutathione levels. The abnormal expression of GGT is significantly associated with features of many metabolic syndromes (e.g., hepatocellular carcinoma). Therefore, it is essential to develop methods to detect GGT so as to monitor the physiological or pathological phenomena related to this species. In this work, by making use of a complex formed by Cu(2+) and glutathione, which may exhibit excellent voltammetric response, we have proposed a novel potential electrochemical method for the detection of the enzyme. Results show that in the presence of GGT, the formation of Cu(2+)-glutathione complex on a working electrode will be disrupted, resulting in greatly depressed electrochemical signals. The primary method exhibits some advantages, such as it being fast, cost-efficient, and conveniently operated. It also has the potential to be further developed as an effective method in the quantitative detection of GGT in real samples.
authors.affiliation: Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Guifang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Shengfa
authors.initials: S
authors.lastname: Ni
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000818:Animals; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005978:Glutathione / Q000378:metabolism; D006736:Horses; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
chemical_list: D005723:gamma-Glutamyltransferase; D005978:Glutathione