title: [Mifepristone inhibits the progesterone-induced expressions of HLA-G, -E, -F genes in trophoblasts during first trimester].
abstract: OBJECTIVE
  To explore the effects of mifepristone on the expressions of HLA-G, HLA-E and HLA-F in extravillous cytotrophoblasts (EVCT).
  
  
  METHODS
  Cultured EVCT during the first trimester were treated in vitro with progesterone and mifepristone. And the transcription levels of HLA-G, HLA-E and HLA-F genes were detected by RT-PCR (reverse transcription-polymerase chain reaction).
  
  
  RESULTS
  As compared with the controls, the levels of HLA-G, HLA-E and HLA-F mRNA significantly increased in EVCT after incubating with 0.1 ng/L progesterone (P < 0.05). These effects could be completely blocked by a co-incubation with mifepristone at the same concentrations.
  
  
  CONCLUSIONS
  Mifepristone can inhibit the effects of progesterone by down-regulating the expressions of HLA-G, HLA-E and HLA-F mRNA in trophoblasts during the first trimester.
authors.affiliation: Huazhong University of Science & Technology, Wuhan, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zeng
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Liao
mesh_terms: D002478:Cells, Cultured; D005260:Female; D059951:HLA-G Antigens / Q000235:genetics / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism*; D000097625:HLA-E Antigens
chemical_list: D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D015735:Mifepristone; D011374:Progesterone; D012333:RNA, Messenger; D000097625:HLA-E Antigens; C062341:HLA-F antigens