title: Biomolecule analyses in an open-tubular capillary chromatography using ternary mixed carrier solvents with chemiluminescence detection.
abstract: We examined the elution behavior of isoluminol isothiocyanate (ILITC)-labeled biomolecules (α-amino acids, peptides, and proteins) in an open-tubular capillary chromatography system using an untreated fused-silica capillary tube and a water-acetonitrile-ethyl acetate mixture carrier solution.  Such an open-tubular capillary chromatography is called "tube radial distribution chromatography (TRDC)" for convenience.  A mixture of ILITC and ILITC-labeled biomolecules was analyzed using TRDC with chemiluminescence detection that provided simple instrument without a light source and complex optical devises.  The ILITC and the labeled twenty α-amino acids were separated, in this order or the reverse order, or not separated with an organic solvent-rich and water-rich carrier solution.  Their elution behavior was considered to be of hydrophilic or hydrophobic nature of ILITC and the labeled α-amino acids.  The ILITC and the labeled protein, alcohol dehydrogenase and bovine serum albumin, were separated in this order with an organic solvent-rich carrier solution, while they were eluted in the reverse order with a water-rich carrier solution, based on the TRDC separation performance.  The TRDC system worked with the untreated open-tubular capillary tube not using any specific capillary tubes, such as coated, packed, or monolithic.
authors.affiliation: Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University, Kyotanabe, Kyoto, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Takahashi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Masuhara
authors.forename: Naoya
authors.initials: N
authors.lastname: Jinno
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tsukagoshi
mesh_terms: D000465:Algorithms; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002623:Chemistry Techniques, Analytical; D019075:Electrophoresis, Capillary / Q000379:methods*; D004867:Equipment Design; D049449:Luminescence; D008956:Models, Chemical; D009930:Organic Chemicals / Q000032:analysis; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000596:Amino Acids; D009930:Organic Chemicals; D010455:Peptides; D011506:Proteins; D012997:Solvents; D014867:Water; D012710:Serum Albumin, Bovine