title: A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum.
abstract: A sensitive and selective method using 2-(7H-dibenzo[a,g]carbazol-7-yl)ethyl 4-methylbenzenesulfonate (DBCETS) as a new fluorescent labeling reagent has been proposed for simultaneously detecting BA and FFA by HPLC with fluorescence detector. The developed method offered the low detection limits of 0.42-0.70 and 0.28-0.57 ng/mL for BA and FFA, respectively. Compared with the reported methods, the proposed method here is capable of offering higher detection sensitivity and selectivity, with less cost and lower volume of sample preparation. This method was validated to ensure high accuracy and precision, and the reliability of its results. When applied to the serum samples of healthy volunteers and patients with hepatic carcinoma, it showed excellent applicability.
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, PR China. guolianglibest@yahoo.com.cn
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Li
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.forename: Yue-Qiu
authors.initials: YQ
authors.lastname: Liu
authors.forename: Nian-Hua
authors.initials: NH
authors.lastname: Jing
authors.forename: Jin-Mao
authors.initials: JM
authors.lastname: You
mesh_terms: D001647:Bile Acids and Salts / Q000097:blood*; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005456:Fluorescent Dyes; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008113:Liver Neoplasms / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001647:Bile Acids and Salts; D005230:Fatty Acids, Nonesterified; D005456:Fluorescent Dyes