title: [Stability evaluation of chemical compositions of Rhizoma gastrodiae with 60Co-γ irradiation by high performance liquid chromatography].
abstract: The main chromatographic fingerprint peaks of Rhizoma gastrodiae were established for evaluating the stability of chemical compositions of Rhizoma gastrodiae with 60Co-gamma irradiation to control the dosage of 60Co-gamma irradiation sterilization by high performance liquid chromatography (HPLC). Eight Rhizoma gastrodiae samples were analyzed. The chromatographic fingerprints of Rhizoma gastrodiae were constructed with 14 common fingerprint peaks, and its 10 main peaks were identified using the similarity evaluation system of chromatographic fingerprints of traditional Chinese medicine (version 2004 A) recommended by State Food and Drug Administration of China. The relative standard deviations of the peak areas of the 10 main peaks were used to evaluate the stability of corresponding chemical compositions of Rhizoma gastrodiae with 60Co-gamma irradiation. The results showed that the compositions of 3 of the 10 main peaks were influenced and a good relationship was obtained between the influence and 60Co-gamma irradiation doses for the eight Rhizoma gastrodiae samples. Some chemical components of Rhizoma gastrodiae weren't steady after 60Co-gamma irradiation, so a low dosage should be selected for 60Co-gamma irradiation sterilization.
authors.affiliation: College of Chemistry and Life Sciences, Leshan Normal University, Leshan 614004, China. yangxy22@lstc.edu.cn
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Yang
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.forename: Sujun
authors.initials: S
authors.lastname: Liu
authors.forename: Jiuhua
authors.initials: J
authors.lastname: Song
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003037:Cobalt Radioisotopes; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000528:radiation effects; D005720:Gamma Rays; D031668:Gastrodia / Q000737:chemistry* / Q000528:radiation effects*; D018517:Plant Roots / Q000737:chemistry / Q000528:radiation effects
chemical_list: D003037:Cobalt Radioisotopes; D004365:Drugs, Chinese Herbal