title: Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus.
abstract: A sensitive membrane-based electrochemical nanobiosensor is developed for the detection of dengue type 2 virus (DENV-2) using nanoporous alumina-modified platinum electrode. Its sensing mechanism relies on the monitoring of electrode's Faradaic current response toward redox probe, ferrocenemethanol, which is sensitive toward the formation of immune complexes within the alumina nanochannels. Anti-DENV-2 monoclonal antibody (clone 3H5, isotype IgG) is used as the biorecognition element in this work. The stepwise additions of antibody, bovine serum albumin (BSA) and DENV-2 are characterized by differential pulse voltammetry (DPV). A low detection limit of 1 pfu mL(-1) with linear range from 1 to 10(3) pfu mL(-1) (R(2)=0.98) can be achieved by the nanobiosensor. The nanobiosensor is selective toward DENV-2 with insignificant cross reaction with non-specific viruses, Chikungunya virus, West Nile virus and dengue type 3 virus (DENV-3). Relative standard deviation (RSD) for triplicate analysis of 5.9% indicates an acceptable level of reproducibility. The first direct quantitation of DENV-2 concentration in whole mosquito vector is demonstrated using this electrochemical nanobiosensor.
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Ming Soon
authors.initials: MS
authors.lastname: Cheng
authors.forename: Jia Shin
authors.initials: JS
authors.lastname: Ho
authors.forename: Cheong Huat
authors.initials: CH
authors.lastname: Tan
authors.forename: Jeslyn Pei Sze
authors.initials: JP
authors.lastname: Wong
authors.forename: Lee Ching
authors.initials: LC
authors.lastname: Ng
authors.forename: Chee-Seng
authors.initials: CS
authors.lastname: Toh
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002417:Cattle; D003715:Dengue / Q000175:diagnosis; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification*; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D057230:Limit of Detection; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D008567:Membranes, Artificial; D012710:Serum Albumin, Bovine; D010984:Platinum; D000537:Aluminum Oxide