title: Effect of heparin-superoxide dismutase on γ-radiation induced DNA damage in vitro and in vivo.
abstract: The effects of heparin-superoxide dismutase (SOD) conjugate (heparin-SOD) on γ-radiation induced DNA damage in vivo and in vitro were evaluated. Plasmid pcDNA3.0 solution was mixed with heparin-SOD, SOD, and a mixture of heparin and SOD (heparin + SOD), respectively, and irradiated with (60)Co at a dosage of 120 Gy. DNA injury was analyzed using agarose gel electrophoresis. The results showed that the degree of injury of pcDNA3.0 mixed with heparin-SOD, SOD, or heparin + SOD was less than that of untreated pcDNA3.0, and among them the degree of injury of pcDNA3.0 mixed with heparin-SOD was the least. It also showed that the protective effect increased with an increase of heparin-SOD concentration. The effects of SOD and heparin-SOD on the DNA damage and tumor inhibition rate of (60)Co γ-radiation exposure on tumor-bearing mice were also studied. Agarose gel electrophoresis showed that, when different SOD samples were administered before irradiation, the thymus DNA injuries of heparin-SOD, SOD, or heparin + SOD groups were more serious than that of the control group, and the DNA injuries of heparin-SOD or heparin + SOD groups were the most serious, which contradicted the above in vitro experiments. However, when heparin-SOD was administered post irradiation, it showed a repairing effect on the injured DNA.
authors.affiliation: Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Shandong University, Ji 'nan, Shandong, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: H N
authors.initials: HN
authors.lastname: Tan
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: Y G
authors.initials: YG
authors.lastname: Wang
authors.forename: R Q
authors.initials: RQ
authors.lastname: Chen
authors.forename: J C
authors.initials: JC
authors.lastname: Cao
authors.forename: F S
authors.initials: FS
authors.lastname: Wang
mesh_terms: D000818:Animals; D004247:DNA; D004249:DNA Damage / Q000187:drug effects; D005720:Gamma Rays; D006493:Heparin; D013482:Superoxide Dismutase
chemical_list: D006493:Heparin; D004247:DNA; D013482:Superoxide Dismutase