title: Cep57, a NEDD1-binding pericentriolar material component, is essential for spindle pole integrity.
abstract: Formation of a bipolar spindle is indispensable for faithful chromosome segregation and cell division. Spindle integrity is largely dependent on the centrosome and the microtubule network. Centrosome protein Cep57 can bundle microtubules in mammalian cells. Its related protein (Cep57R) in Xenopus was characterized as a stabilization factor for microtubule-kinetochore attachment. Here we show that Cep57 is a pericentriolar material (PCM) component. Its interaction with NEDD1 is necessary for the centrosome localization of Cep57. Depletion of Cep57 leads to unaligned chromosomes and a multipolar spindle, which is induced by PCM fragmentation. In the absence of Cep57, centrosome microtubule array assembly activity is weakened, and the spindle length and microtubule density decrease. As a spindle microtubule-binding protein, Cep57 is also responsible for the proper organization of the spindle microtubule and localization of spindle pole focusing proteins. Collectively, these results suggest that Cep57, as a NEDD1-binding centrosome component, could function as a spindle pole- and microtubule-stabilizing factor for establishing robust spindle architecture.
authors.affiliation: The State Key Laboratory of Biomembrane and Membrane Bioengineering and The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Qixi
authors.initials: Q
authors.lastname: Wu
authors.forename: Runsheng
authors.initials: R
authors.lastname: He
authors.forename: Haining
authors.initials: H
authors.lastname: Zhou
authors.forename: Albert C H
authors.initials: AC
authors.lastname: Yu
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Junlin
authors.initials: J
authors.lastname: Teng
authors.forename: Jianguo
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D016466:CHO Cells; D018385:Centrosome / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism*; D014981:Xenopus / Q000378:metabolism
chemical_list: C474610:CEP57 protein, human; D008869:Microtubule-Associated Proteins; C509189:NEDD1 protein, human; D009687:Nuclear Proteins; D034741:RNA, Small Interfering