title: Acellular bone colonization and aggregate culture conditions diversely influence murine periosteum mesenchymal stem cell differentiation potential in long-term in vitro osteoinductive conditions.
abstract: Periosteum contains mesenchymal stem cells (Pe-MSCs) that contribute to normal bone growth, healing, and turnover; understanding Pe-MSC capabilities may shed light over the treatment of bone defects using tissue engineering. Bone tissue regeneration needs in vitro bone precursors or stem cell coculture onto specific scaffolds but, despite extensive research in the field, very little is known about the matrix structure of the tissue-engineered tissues and the scaffold's effects on cell differentiation. To this purpose we have selected a clonal population (murine Pe-MSCs) that was seeded and differentiated onto an acellular bone scaffold. Cell differentiation was assessed after 3 months and 1 year by molecular, histological, biochemical, and biophysical analyses and results were compared with the same osteoinduced clonal cells cultured as cellular aggregates. Our data show that Pe-MSCs cultured onto acellular bone scaffold develop a complex three-dimensional matrix and an osteoblastic phenotype but do not produce hydroxyapatite (HA); moreover, they seem able to reabsorb the colonized bone scaffold. On the contrary, cells cultured as three-dimensional aggregates differentiate and produce osteoblastic markers and HA nanocrystals.
authors.affiliation: Dipartimento di Scienze Mediche e Biologiche, Università di Udine, Piazzale M. Kolbe 4, Udine, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Ferro
authors.forename: Renza
authors.initials: R
authors.lastname: Spelat
authors.forename: Federica
authors.initials: F
authors.lastname: D'Aurizio
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Falini
authors.forename: Ilaria
authors.initials: I
authors.lastname: De Pol
authors.forename: Maura
authors.initials: M
authors.lastname: Pandolfi
authors.forename: Antonio Paolo
authors.initials: AP
authors.lastname: Beltrami
authors.forename: Daniela
authors.initials: D
authors.lastname: Cesselli
authors.forename: Carlo Alberto
authors.initials: CA
authors.lastname: Beltrami
authors.forename: Francesco
authors.initials: F
authors.lastname: Curcio
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002449:Cell Aggregation; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D048430:Cell Shape; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010521:Periosteum / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D013194:Staining and Labeling; D013997:Time Factors