title: GSTP1 gene methylation profiles in Helicobacter pylori (+) and (-) antral intestinal metaplasia and distal gastric tumour patients in Turkish population.
abstract: OBJECTIVE
  Gastric cancer (GC) is the second most common malignancy worldwide, with a high mortality rate. The incidence of GC has declined in the western countries during the last decades. The glutathione S-transferases comprise a group of enzymes that are critical in the detoxification of carcinogens. In this study we aimed at the relationship GSTP-1 methylation in patients with intestinal metaplasia with and without Helicobacter pylori infection, gastric cancer and controls.
  
  
  METHODS
  The methylation status of GSTP1 gene was analyzed by methylation specific PCR after bisulfate modification in H. pylori (+) (n=25) and (-) (n=25) intestinal metaplasia (IM) patients, GC (n=25) and control subjects (n=15) between September 2009 to November 2011.
  
  
  RESULTS
  During the study period 90 patients who underwent endoscopic examination were included in the study. When we considered the GSTP1 gene methylation profile in all of the groups; 26 (28%)patients had methylated GSTP1 gene, 31 (34%) patients had unmethylated GSTP1 gene and 33 (36%) patients had heterogeneously methylated GSTPI gene.
  
  
  CONCLUSIONS
  GSTP1 gene methylation profile is not appropriate for early diagnosis of cases with gastric cancer.
authors.affiliation: Gastroenterology Department, Celal Bayar University Medical School, Manisa, Turkey.
authors.forename: Gulcan
authors.initials: G
authors.lastname: Asik-Sen
authors.forename: Elmas
authors.initials: E
authors.lastname: Kasap
authors.forename: Seda
authors.initials: S
authors.lastname: Orenay-Boyacioglu
authors.forename: Mehmet
authors.initials: M
authors.lastname: Korkmaz
authors.forename: Erkan
authors.initials: E
authors.lastname: Kahraman
authors.forename: Belkis
authors.initials: B
authors.lastname: Unsal
authors.forename: Elif
authors.initials: E
authors.lastname: Yuksel-Saritas
authors.forename: Hakan
authors.initials: H
authors.lastname: Yuceyar
mesh_terms: D000328:Adult; D001706:Biopsy; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D019175:DNA Methylation; D055088:Early Detection of Cancer; D005260:Female; D005773:Gastroscopy; D020022:Genetic Predisposition to Disease; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D016481:Helicobacter Infections / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008679:Metaplasia; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011379:Prognosis; D011706:Pyloric Antrum / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D014421:Turkey
chemical_list: C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi