title: Adipose-derived stem-cell treatment of skeletal muscle injury.
abstract: BACKGROUND
  The aim of the present study was to investigate whether adipose-derived stem cells could contribute to skeletal muscle-healing.
  
  
  METHODS
  Adipose-derived stem cells of male rats were cultured and injected into the soleus muscles of female rats. Two and four weeks after injections, muscles were tested for tetanic force (50 Hz). Histological analysis was performed to evaluate muscle collagen deposition and the number of centronucleated muscle fibers. In order to track donor cells, chimerism was detected with use of real-time polymerase chain reaction targeting the male sex-determining region Y (SRY) gene.
  
  
  RESULTS
  Two weeks after cell injection, tetanus strength and the number of centronucleated regenerating myofibers, as well as the number of centronucleated regenerating myofibers, were higher in the treated group than they were in the control group (mean and standard error of the mean, 79.2 ± 5.0% versus 58.3 ± 8.1%, respectively [p < 0.05]; and 145 ± 36 versus 273 ± 18 per 10³ myofibers, respectively [p < 0.05]). However, there were no significant differences at four weeks. Treatment did not decrease collagen deposition. Male gene was not detected in female host tissue at two and four weeks after engraftment by polymerase chain reaction analysis.
  
  
  CONCLUSIONS
  Adipose-derived stem-cell therapy increased muscle repair and force at two weeks, but not four weeks, after injection, suggesting that adipose-derived stem-cell administration may accelerate muscle repair; however, the rapid disappearance of injected cells suggests a paracrine mechanism of action.
authors.affiliation: Escola de Educação Física e Desportos-CCS, Laboratório de Biologia do Exercício, Departamento de Biociência e Atividade Física, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 540 Ilha do Fundão, Rio de Janeiro, 21941-599, Brazil.
authors.forename: Ramon
authors.initials: R
authors.lastname: Peçanha
authors.forename: Luiza de Lima E Silva
authors.initials: LL
authors.lastname: Bagno
authors.forename: Marcelo Baldanza
authors.initials: MB
authors.lastname: Ribeiro
authors.forename: Anna Beatriz
authors.initials: AB
authors.lastname: Robottom Ferreira
authors.forename: Milton Ozório
authors.initials: MO
authors.lastname: Moraes
authors.forename: Gisele
authors.initials: G
authors.lastname: Zapata-Sudo
authors.forename: Taís Hanae
authors.initials: TH
authors.lastname: Kasai-Brunswick
authors.forename: Antônio Carlos
authors.initials: AC
authors.lastname: Campos-de-Carvalho
authors.forename: Regina Coeli dos Santos
authors.initials: RC
authors.lastname: Goldenberg
authors.forename: João Pedro
authors.initials: JP
authors.lastname: Saar Werneck-de-Castro
mesh_terms: D017667:Adipocytes / Q000637:transplantation*; D000704:Analysis of Variance; D000818:Animals; D001707:Biopsy, Needle; D004195:Disease Models, Animal; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D007150:Immunohistochemistry; D008297:Male; D009119:Muscle Contraction / Q000502:physiology; D053580:Muscle Strength / Q000502:physiology; D018482:Muscle, Skeletal / Q000293:injuries* / Q000473:pathology / Q000601:surgery; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012038:Regeneration / Q000502:physiology; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D033581:Stem Cell Transplantation / Q000379:methods*; D014947:Wounds and Injuries / Q000473:pathology / Q000628:therapy*