title: Microchip device with 64-site electrode array for multiplexed immunoassay of cell surface antigens based on electrochemiluminescence resonance energy transfer.
abstract: This paper describes a novel on-chip microarray platform based on an electrochemiluminescence resonance energy transfer (ECL-RET) strategy for rapid assay of cancer cell surface biomarkers. This platform consists of 64 antigen-decorated CdS nanorod spots with the diameter of 1.0 cm uniformly distributed on 16 indium tin oxide (ITO) strips, which is coated with a multichannel decorated polydimethylsiloxane (PDMS) slice to realize multiplexed determination of antigens. To shorten the immune reaction time in the microchannels and simplify the device, magnetic stirring and four-channel universal serial bus (USB) ports for plug-and-play were used. When Ru(bpy)(3)(2+) labeled antibodies were selectively captured by the corresponding antigens on the CdS nanorod spot array, ECL-RET from the CdS nanorod (donor) by cathodic emission in the presence of K(2)S(2)O(8) to Ru(bpy)(3)(2+) (acceptor) occurred. With signal amplification of Ru(bpy)(3)(2+) and competitive immunoassay, carcinoembryonic antigen (CEA), α-fetoprotein (AFP), and prostate specific antigen (PSA) as models were detected on this microfluidic device via recording the increased ECL-RET signals on electrode surfaces. Furthermore, this multiplexed competitive immunoassay was successfully used for detecting cancer cell surface antigens via the specific antibody-cell interactions and cell counting via cell surface receptors and antigens on the CdS nanorod surface. This platform provides a rapid and simple but sensitive approach with microliter-level sample volume and holds great promise for multiplexed detection of antigens and antigen-specific cells.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Mei-Sheng
authors.initials: MS
authors.lastname: Wu
authors.forename: Hai-Wei
authors.initials: HW
authors.lastname: Shi
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: He
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D019187:Cadmium Compounds / Q000737:chemistry; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000276:immunology; D045744:Cell Line, Tumor; D055664:Electrochemical Techniques / Q000191:economics / Q000295:instrumentation; D004566:Electrodes; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000295:instrumentation*; D056656:Lab-On-A-Chip Devices; D008163:Luminescent Measurements / Q000191:economics / Q000295:instrumentation; D008297:Male; D043942:Nanotubes / Q000737:chemistry / Q000648:ultrastructure; D009369:Neoplasms / Q000175:diagnosis; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000276:immunology; D012680:Sensitivity and Specificity; D013440:Sulfides / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D019187:Cadmium Compounds; D002272:Carcinoembryonic Antigen; D013440:Sulfides; D000509:alpha-Fetoproteins; C034939:cadmium sulfide; D017430:Prostate-Specific Antigen