title: [Screening and identification of natural antisense transcript in mouse cerebral cortex].
abstract: OBJECTIVE
  To screen and identify the possible existence of natural antisense transcript (NAT) within the mouse neocortex.
  
  
  METHODS
  Sixty-three cerebral cortex layer-specific genes were screened by bioinformatics prediction in mice, among which 31 mice with potential NATs were screened. NAT was identified using reverse transcription polymerase chain reaction (RT-PCR) and then cloned in pGEM-T Vector System for sequencing.
  
  
  RESULTS
  Among 31 genes predicted using bioinformatics, 8 were proved to be NAT positive by RT-PCR.
  
  
  CONCLUSIONS
  NATs exist in the mouse neocortex tissue during the development of cerebral cortex. NATs may influence mouse cortical development by regulating the related coding genes.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Li
authors.forename: Shan
authors.initials: S
authors.lastname: Wang
authors.forename: Xiao-Chao
authors.initials: XC
authors.lastname: Tan
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
authors.forename: Xiao-Zhong
authors.initials: XZ
authors.lastname: Peng
mesh_terms: D000818:Animals; D002460:Cell Line; D002540:Cerebral Cortex; D051379:Mice; D008969:Molecular Sequence Data; D016372:RNA, Antisense / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D016372:RNA, Antisense; D012333:RNA, Messenger