title: Infection of chickens caused by avian influenza virus A/H5N1 delivered by aerosol and other routes.
abstract: This study presents results of the study of infectivity of avian influenza virus (AIV) A subtype H5N1 strains isolated from agricultural birds across the territory of the Russian Federation and CIS countries. The results of the susceptibility of chickens to the AIV isolates delivered by the aerosol route and the dissemination of the virus in the organs of infected birds are presented. As was observed, the sensitivity of birds to AIV by the aerosol route of infection is 30 times higher than by intranasal route, 500 times higher than by the oral route and 10000 times higher than by the intragastric route of infection, which is indicative of higher permissivity of respiratory organs to AIV. The highest titres of AIV A subtype H5N1(A/Chicken/Kurgan/05/2005 strain) in aerosol-infected chickens were found in nasal cavity mucosa, lungs, cloaca, serum and kidney, where viable virus accumulation was detected by 18h post-infection (p.i.). The highest virus titres were observed 54h p.i. in lungs, serum and kidney, reaching the value of 8.16 lg EID50 /g(ml) in the lungs. The results showed that birds infected by the aerosol route developed higher titres of virus than those infected by other routes.
authors.affiliation: State Research Centre of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk region, Russia.
authors.forename: Ar A
authors.initials: AA
authors.lastname: Sergeev
authors.forename: O K
authors.initials: OK
authors.lastname: Demina
authors.forename: O V
authors.initials: OV
authors.lastname: Pyankov
authors.forename: O G
authors.initials: OG
authors.lastname: Pyankova
authors.forename: A P
authors.initials: AP
authors.lastname: Agafonov
authors.forename: S A
authors.initials: SA
authors.lastname: Kiselev
authors.forename: I E
authors.initials: IE
authors.lastname: Agranovski
authors.forename: Al A
authors.initials: AA
authors.lastname: Sergeev
authors.forename: A N
authors.initials: AN
authors.lastname: Shikov
authors.forename: L N
authors.initials: LN
authors.lastname: Shishkina
authors.forename: A S
authors.initials: AS
authors.lastname: Safatov
authors.forename: A N
authors.initials: AN
authors.lastname: Sergeev
mesh_terms: D000281:Administration, Intranasal; D000284:Administration, Oral; D000336:Aerosols; D000818:Animals; D002645:Chickens / Q000821:virology*; D041981:Gastrointestinal Tract / Q000821:virology; D053124:Influenza A Virus, H5N1 Subtype / Q000302:isolation & purification / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000473:pathology / Q000821:virology*; D007668:Kidney / Q000473:pathology / Q000821:virology*; D008168:Lung / Q000473:pathology / Q000821:virology*; D012426:Russia
chemical_list: D000336:Aerosols