title: [Simultaneous determination of five main index components and specific chromatograms analysis in Xiaochaihu granules].
abstract: Reversed phase high performance liquid chromatography with diode array detector was employed for simultaneous determination of five main index components and specific chromatograms analysis in Xiaochaihu granules with a linear gradient elution of acetonitrile-water (containing 0.1% phosphoric acid) as mobile phase. The results showed that five main index components (baicalin, baicalein, wogonoside, wogonin, enoxolone) were separated well under the analytical condition. The linear ranges of five components were 0.518 - 16.576, 0.069 - 2.197, 0.167 - 5.333, 0.009 - 0.297 and 0.006 - 0.270 mg x g(-1), respectively. The correlation coefficients were 0.999 9, and the average recoveries ranged from 95% to 105%. Twelve common peaks were selected as the specific chromatograms of Xiaochaihu granules with baicalin as the reference peak. There were good similarities between the reference and the ten batches of samples. The similarity coefficients were no less than 0.9. The analytical method established is highly sensitive with strong specificity and it can be used efficiently in the quality control of Xiaochaihu granules.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Yan-Shuang
authors.initials: YS
authors.lastname: Zhuang
authors.forename: Hao
authors.initials: H
authors.lastname: Cai
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Cai
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D044950:Flavanones / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D006034:Glycyrrhetinic Acid / Q000032:analysis*; D011786:Quality Control; D012680:Sensitivity and Specificity
chemical_list: D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; C550200:xiaochaihu; C038044:baicalin; C006680:baicalein; C473995:wogonoside; D006034:Glycyrrhetinic Acid; C085514:wogonin