title: A novel monoclonal antibody against cannabinoid receptor 1.
abstract: The cannabinoid receptor 1 (CBR1) is being widely investigated because of its specific structure and functions compared with other cannabinoid receptors. In this study, we immunized BALB/c mice with synthesized human CBR1 polypeptide and obtained a novel monoclonal antibody (MAb) against human CBR1. Analysis through enzyme-linked immunosorbent assay (ELISA), spot-ELISA, Western blot, and immunohistochemistry revealed that the MAb was specifically against recombinant human CBR1 protein, and its subtype and affinity constant (Kaff) were IgG2b/k and 7.85 × 10(8) M/L, respectively. Using this MAb we found that CBR1 is expressed on HL-7702 cells and lipid tissue, raising the possibility that the CBR1 may take a role in glucose and lipid metabolism. Thus, this antibody might facilitate studies for pathophysiology of diseases associated with glucose and lipid metabolism abnormality.
authors.affiliation: Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Xinglin
authors.initials: X
authors.lastname: Kuang
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Liu
authors.forename: Yilan
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Luo
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wei
authors.forename: Jianhong
authors.initials: J
authors.lastname: Bi
authors.forename: Chongjie
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D043882:Receptors, Cannabinoid / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D043882:Receptors, Cannabinoid; D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; C555915:carbonyl reductase 1, mouse; C531129:CBR1 protein, human