title: Hyperglycemia and oxidative stress in cultured endothelial cells--a comparison of primary endothelial cells with an immortalized endothelial cell line.
abstract: Diabetes mellitus is a major risk factor for the development of cardiovascular disease and oxidative stress plays an important role in this process. Therefore, we investigated the effects of hyperglycemia on the formation of reactive oxygen species (ROS) and nitric oxide/cGMP signaling in two different endothelial cell cultures. Human umbilical vein endothelial cells (HUVEC) and EA.hy 926 cells showed increased oxidative stress and impaired NO-cGMP signaling in response to hyperglycemia. The major difference between the two different cell types was the dramatic decrease in viability in HUVEC whereas EA.hy cells showed rather increased growth under hyperglycemic conditions. Starvation led to an additional substantial decrease in viability and increased superoxide formation in HUVEC. Both endothelial cell types, HUVEC and EA.hy 926, may be used as models for vascular hyperglycemia. However, high growth medium should be used to avoid starvation-induced oxidative stress and cell death.
authors.affiliation: 2nd Medical Clinic, Department of Cardiology, Medical Center of the Johannes Gutenberg University, Mainz, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Karbach
authors.forename: Thomas
authors.initials: T
authors.lastname: Jansen
authors.forename: Sven
authors.initials: S
authors.lastname: Horke
authors.forename: Tjebo
authors.initials: T
authors.lastname: Heeren
authors.forename: Alexander
authors.initials: A
authors.lastname: Scholz
authors.forename: Meike
authors.initials: M
authors.lastname: Coldewey
authors.forename: Angelica
authors.initials: A
authors.lastname: Karpi
authors.forename: Michael
authors.initials: M
authors.lastname: Hausding
authors.forename: Swenja
authors.initials: S
authors.lastname: Kröller-Schön
authors.forename: Matthias
authors.initials: M
authors.lastname: Oelze
authors.forename: Thomas
authors.initials: T
authors.lastname: Münzel
authors.forename: Andreas
authors.initials: A
authors.lastname: Daiber
mesh_terms: D016923:Cell Death / Q000187:drug effects; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D005947:Glucose / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D006943:Hyperglycemia / Q000150:complications / Q000473:pathology*; D009569:Nitric Oxide / Q000494:pharmacology; D018384:Oxidative Stress / Q000502:physiology*; D061251:Primary Cell Culture; D017382:Reactive Oxygen Species / Q000494:pharmacology*
chemical_list: D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D006152:Cyclic GMP; D005947:Glucose