title: [Coumarins of Anemone raddeana Regel and their biological activity].
abstract: To study the coumarins of Anemone raddeana Regel, the compounds were separated by silica gel column chromatography and HPLC. Their structures were identified by their physicochemical property and spectral analysis. Two new compounds were isolated and identified as 4, 7-dimethoxyl-5-methyl-6-hydroxy coumarin (1) and 4, 7-dimethoxyl-5-formyl-6-hydroxycoumarin (2). The bioassays indicated that compounds 1 and 2 could significantly inhibit the proliferation of cancer cell, and showed the agonist effect on the transactivity of retinoic acid receptor-alpha (RARalpha). In addition, the two compounds had inhibitory effect against human leukocyte elastase (HLE).
authors.affiliation: New Drug Research and Development Center of North China Pharmaceutical Group Corporation, National Microbial Medicine Engineering and Research Center, Shijiazhuang 050015, China.
authors.forename: Feng-Zhi
authors.initials: FZ
authors.lastname: Ren
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Chen
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Zheng
authors.forename: Xue-Xia
authors.initials: XX
authors.lastname: Zhang
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Li
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Dong
mesh_terms: D031883:Anemone / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D019272:Leukocyte Elastase / Q000378:metabolism; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism; D000072482:Retinoic Acid Receptor alpha; D027343:Rhizome / Q000737:chemistry; D015533:Transcriptional Activation
chemical_list: C582237:4,7-dimethoxyl-5-formyl-6-hydroxycoumarin; C582236:4,7-dimethoxyl-5-methyl-6-hydroxycoumarin; D000972:Antineoplastic Agents, Phytogenic; D003374:Coumarins; C000607071:RARA protein, human; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; D019272:Leukocyte Elastase