title: Procaterol-stimulated increases in ciliary bend amplitude and ciliary beat frequency in mouse bronchioles.
abstract: The beating cilia play a key role in lung mucociliary transport. The ciliary beating frequency (CBF) and ciliary bend amplitude (CBA) of isolated mouse bronchiolar ciliary cells were measured using a light microscope equipped with a high-speed camera (500 Hz). Procaterol (aβ(2)-agonist) increased CBA and CBF in a dose dependent manner via cAMP. The time course of CBA increase is distinct from that of CBF increase: procaterol at 10 nM first increased CBA and then CBF. Moreover, 10 pM procaterol increased CBA, not CBF, whereas 10 nM procaterol increased both CBA and CBF. Concentration-response studies of procaterol demonstrated that the CBA curve was shifted to a lower concentration than the CBF curve, which suggests that CBA regulation is different from CBF regulation. Measurements of microbead movements on the bronchiole of lung slices revealed that 10 pM procaterol increased the rate of ciliary transport by 37% and 10 nM procaterol increased it by 70%. In conclusion, we have shown that increased CBA is of particular importance for increasing the bronchiolar ciliary transport rate, although CBF also plays a role in increasing it.
authors.affiliation: Nakahari Project of Central Research Laboratory, Osaka Medical College, Takatsuki, Japan.
authors.forename: Nobuyo
authors.initials: N
authors.lastname: Komatani-Tamiya
authors.forename: Eriko
authors.initials: E
authors.lastname: Daikoku
authors.forename: Yoshizumi
authors.initials: Y
authors.lastname: Takemura
authors.forename: Chikao
authors.initials: C
authors.lastname: Shimamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakano
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kohda
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Marunaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakahari
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000494:pharmacology; D000420:Albuterol / Q000494:pharmacology; D000818:Animals; D054468:Axoneme / Q000378:metabolism / Q000502:physiology; D055745:Bronchioles / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D002118:Calcium / Q000494:pharmacology; D002923:Cilia / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008863:Microspheres; D009079:Mucociliary Clearance; D017265:Procaterol / Q000494:pharmacology*; D013997:Time Factors; D014741:Video Recording
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D000242:Cyclic AMP; D000420:Albuterol; D002118:Calcium; D017265:Procaterol