title: HPV-16 E6 and E7 oncogene expression is downregulated as a result of             Mdm2 knockdown.
abstract: The carcinogenic potential of HPV infections is based on the integration             and constitutive expression of the E6 and E7 genes which inhibit the p53 and Rb             tumor suppressor proteins. In normal cells, Mdm2 regulates p53 in a negative feedback             loop, and although Mdm2 is apparently functional in HPV-infected cells, E6 is             the protein responsible for repressing p53 replacing Mdm2 function. The role of             Mdm2 in HPV-positive cells is still elusive. In this study, Mdm2 was knocked down             in an HPV-positive cervical cancer cell line; as a result we found downregulation             of the expression of E6 and E7 and p53 upregulation.
authors.affiliation: Department of Clinic Analysis and Molecular Diagnostic, Faculty of Chemistry, Universidad Autónoma de Coahuila, Republica Oriente, CP 25280, Saltillo, Coahuila, Mexico. daniel.diazg@gmail.com
authors.forename: Daniel
authors.initials: D
authors.lastname: Diaz
authors.forename: Mauricio A Salinas
authors.initials: MA
authors.lastname: Santander
authors.forename: Jesús A Morlett
authors.initials: JA
authors.lastname: Chavez
mesh_terms: D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D054884:Host-Pathogen Interactions; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D050725:Papillomavirus E7 Proteins / Q000235:genetics* / Q000378:metabolism; D030361:Papillomavirus Infections / Q000821:virology; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000821:virology
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C059731:oncogene protein E7, Human papillomavirus type 16; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2