title: The effect of high temperature on the kinetics of lipopolysaccharide (LPS)-induced human monocytes activity in vitro.
abstract: Human peripheral blood monocytes were stimulated with lipopolysaccharide at different temperatures (34, 37 and 40 °C) and TNF-α, IL-1β and NO production was measured. Levels of TNF-α mRNA and IL-1β mRNA were measured by RT-PCR. Phagocytic activity of LPS-stimulated monocytes in terms of bacterial uptake and intracellular bacterial killing was checked at different conditions in vitro. Early elevation of TNF-α, IL-1β and NO production was found in LPS-stimulated monocytes that incubated at 40 °C followed by cells that incubated at 37 °C and lowest level was detected at 34 °C. Similar results were observed in the phagocytic activity. Expression of TNF-α mRNA and IL-1β mRNA was observed as early as 30 min post exposure to LPS in all studied temperatures and these, decreased sharply after 12 h post exposure to LPS in LPS-stimulated monocytes that incubated at 40 °C only. This report describes the striking effects of incubation temperature on activity of LPS-stimulated monocytes.
authors.affiliation: Department of Biology, College of Science, Baghdad University, Baghdad, Iraq. Ayaid_khadem@yahoo.com
authors.forename: Ayaid Khadem
authors.initials: AK
authors.lastname: Zgair
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D006801:Humans; D007700:Kinetics; D008070:Lipopolysaccharides / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D009569:Nitric Oxide / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D013696:Temperature
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D009569:Nitric Oxide