title: Involvement of P2Y13 receptor in suppression of neuronal differentiation.
abstract: We examined the receptor-mediated effects of extracellular ATP on neuronal differentiation of PC12 cells, Neuro2a cells and MEB5 cells by using a series of receptor antagonists. The P2Y13 receptor antagonist MRS2211 significantly accelerated neurite outgrowth in all cases. Treatment with nerve growth factor (NGF) alone activated ERK1/2 in PC12 cells, and the activation was further increased by MRS2211. These results suggest involvement of P2Y13 receptor in suppression of neuronal differentiation. Thus, P2Y13 receptor antagonists might be candidates for treatment of neurodegenerative diseases.
authors.affiliation: Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science (TUS), 3613 Yamazaki, Noda City, Chiba 278-8510, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Yano
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Tsukimoto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Harada
authors.forename: Shuji
authors.initials: S
authors.lastname: Kojima
mesh_terms: D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D051379:Mice; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D020932:Nerve Growth Factor / Q000494:pharmacology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D001391:Azo Compounds; C556691:MRS 2211; C486689:P2ry13 protein, mouse; C486690:P2ry13 protein, rat; D018048:Receptors, Purinergic P2; D011732:Pyridoxal Phosphate; D020932:Nerve Growth Factor; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases