title: Effects of triptolide on the synaptophysin expression of hippocampal neurons in the AD cellular model.
abstract: Due to the immunoinflammatory pathology of Alzheimer's disease (AD) brain, recent studies have begun to focus attention on the role of anti-inflammatory drugs or immunomodulators in AD. Triptolide isolated from the herb Tripterygium wilfordii Hook F has anti-inflammatory and immunosuppressive activities. In this study, we observed the effects of triptolide on synaptophysin expression in AD cellular model. AD cellular model was established by action of Aβ-stimulated microglial conditioned medium (MCM) on cultured rat hippocampal neurons (HN). Immunocytochemical staining, western blot and RT-PCR were used to observe the effects of triptolide at different dosages on the synaptophysin expression of hippocampal neurons in AD cellular model at different time points during incubation of cultures. After 24 h of cultivation, the expression level of synaptophysin in MCM/HN model group was decreased as compared with normal HN group and MCM/HN control group, and the expression level of synaptophysin in MCM/HN low-dose triptolide group and MCM/HN high-dose triptolide group was increased as compared with MCM/HN model group. It is concluded that triptolide can promote the synaptophysin expression of hippocampal neurons in the AD cellular model.
authors.affiliation: Department of Anatomy, Medical College of Nanchang University, Nanchang, Jiangxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Nie
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: Cheng
authors.initials: C
authors.lastname: Lü
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Hu
authors.forename: Bin
authors.initials: B
authors.lastname: Wan
authors.forename: Baolin
authors.initials: B
authors.lastname: Yang
authors.forename: Yaobing
authors.initials: Y
authors.lastname: Li
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000633:toxicity; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001483:Base Sequence; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004195:Disease Models, Animal; D004224:Diterpenes / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D007155:Immunologic Factors / Q000494:pharmacology; D017628:Microglia / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology; D010446:Peptide Fragments / Q000737:chemistry / Q000633:toxicity; D010616:Phenanthrenes / Q000494:pharmacology*; D055503:Protein Multimerization; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D016708:Synaptophysin; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D000894:Anti-Inflammatory Agents, Non-Steroidal; D017077:Culture Media, Conditioned; D004224:Diterpenes; D004852:Epoxy Compounds; D007155:Immunologic Factors; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D010616:Phenanthrenes; D012333:RNA, Messenger; D016708:Synaptophysin; C569016:Syp protein, rat; D033921:Vesicular Transport Proteins; C078592:amyloid beta-protein (1-40); C001899:triptolide