title: Oral treatment with Hev b 13 prevents experimental arthritis in mice.
abstract: Hev b 13 is an allergenic esterase obtained from the rubber tree Hevea brasiliensis, which has been shown recently to induce human monocytes to release interleukin (IL)-10 in vitro, and to exert a potent anti-inflammatory effect in vivo. Moreover, Hev b 13 has been shown to reduce clinical signs of inflammation and also histological damage to the distal colon of mice with 2,4,6-trinitrobenze sulphonic acid (TNBS)-induced colitis after its oral administration. The aim of this study was to investigate the effect of Hev b 13 on human mononuclear cells, as well as its therapeutic use in the methylated bovine serum albumin (mBSA) model of antigen-induced arthritis. Five days before the intra-articular challenge, and daily thereafter for 8 days, Hev b 13 was administered by oral gavage. In mice treated with a dose of 0·5 mg/kg of Hev b 13, the severity of oedema, leucocyte infiltration, pannus formation and cartilage erosion were reduced significantly. These findings underscore the anti-inflammatory activity suggested previously for Hev b 13, an activity speculated to be related to its interaction with monocytes/macrophages and the consequent stimulation of IL-10 release and reduction of tumour necrosis factor (TNF) release. The study also opens a wide range of possible applications in the field of immune-mediated inflammatory diseases.
authors.affiliation: Departments of Biochemistry and Immunology             Surgery and Anatomy             Clinical Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
authors.forename: Larissa
authors.initials: L
authors.lastname: De Bortoli Teixeira
authors.forename: Vera Lúcia Aparecida
authors.initials: VL
authors.lastname: Aguillar Epifânio
authors.forename: João José
authors.initials: JJ
authors.lastname: Lachat
authors.forename: Norma
authors.initials: N
authors.lastname: Tiraboschi Foss
authors.forename: Joaquim
authors.initials: J
authors.lastname: Coutinho-Netto
mesh_terms: D000284:Administration, Oral; D000818:Animals; D052179:Antigens, Plant / Q000008:administration & dosage*; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000517:prevention & control*; D002356:Cartilage / Q000187:drug effects / Q000473:pathology; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D018450:Disease Progression; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000008:administration & dosage*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D052179:Antigens, Plant; C473106:Hev b 13 allergen, Hevea brasiliensis; D010940:Plant Proteins; D014409:Tumor Necrosis Factor-alpha; C029180:methylated bovine serum albumin; D012710:Serum Albumin, Bovine