title: [Effect of irbesartan on the proliferation, apoptosis and VEGF mRNA expression of human umbilical vein cell line ea. hy926 in vitro].
abstract: OBJECTIVE
  To evaluate the effect of irbesartan on the proliferation, apoptosis, and VEGF mRNA expression of human umbilical vein cell line EA.hy926 in vitro.
  
  
  METHODS
  The human umbilical vein cell line EA.hyY926 were treated with various concentrations of irbesartan for 24 h. The cell proliferation after the treatment was detected by CCK8 assay, flow cytometry and FITC Annexin V/PI kit were used to detect changes in the cell apoptosis. RT-PCR was used to evaluate the expression of VEGF mRNA.
  
  
  RESULTS
  There were no changes in cell shape with various concentration of irbesartan. CCK-8 assay showed a greater rate of the cell proliferation in irbesartan group than that in control group with a dose-independent manner after 24 h treatment. After incubation with irbesartan, cell proliferation rate was significant (P < 0.05). FCM analysis showed no significantly changes in the cell apoptosis. Irbesartan increased the proliferation of EA.hy926 cells. At concentration of 1 x 10(-4), 1 x 10(-5), 1 x 10(-6) mol/L, VEGF mRNA expression enhanced either (P < 0.05).
  
  
  CONCLUSIONS
  Irbesartan could promote the proliferation and up-regulated VEGFmRNA expression in EA.hy926 cell line. This result suggested that in addition to antihypertensive effect, angiotensin receptor antagonist might be a novel therapeutic approach to chronic ischemic heart disease as heart failure.
authors.affiliation: Geriatric Department of Cardiology, Institute of Gerontology and Geriatrics, General Hospital of PLA, Beijing 100853, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Ma
authors.forename: Xue-Chun
authors.initials: XC
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D000077405:Irbesartan; D012333:RNA, Messenger / Q000235:genetics; D013777:Tetrazoles / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D001713:Biphenyl Compounds; D012333:RNA, Messenger; D013777:Tetrazoles; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D000077405:Irbesartan