title: [Evaluation of the protective performance of a positive pressure bio-protective clothing against viral aerosol].
abstract: OBJECTIVE
  To evaluate the protective performance of a positive pressure bio-protective clothing against viral aerosol.
  
  
  METHODS
  The suspension of indicating virus phage Phi-X174 was made for viral aerosol generating in a hermetic cabin. The diameter of viral aerosol particles were measured with a aerodynamics size analyzer. By adjusting the inner humidity of the cabin, the protective efficiency of the positive pressure bio-protective clothing against viral aerosol in high and low windshield conditions was determined with Andersen six-stage air sampler sampling and plage forming unit (PFU) counting, respectively.
  
  
  RESULTS
  The mass median diameter of Phage Phi-X174 aerosol particles was about 0.922 µm and the background concentration is beyond 2 × 10⁴ particles/m³. The protective efficiency of the clothing against phage Phi-X174 aerosol particles was above 99.9% under different test conditions with the range of viral aerosol concentration between 0 - 23 PFU/m³. Airflow (P = 0.84), environment humidity conditions (P = 0.33) and sampling time (P = 0.07) did not affect the protective efficiency statistically.
  
  
  CONCLUSIONS
  The positive pressure bio-protective clothing provided a relatively high efficiency against phage Phi-X174 aerosol regardless of airflow rate, environment humidity and sampling time.
authors.affiliation: Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, State Key Laboratory of Pathogen and Biosecurity, National Research Center of Bio-Protection Equipment and Engineering, Beijing 100071, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Zhan-bo
authors.initials: ZB
authors.lastname: Wen
authors.forename: Wen-hui
authors.initials: WH
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Li
authors.forename: Ling-fei
authors.initials: LF
authors.lastname: Hu
authors.forename: Xiao-kai
authors.initials: XK
authors.lastname: Dong
authors.forename: Ke-yang
authors.initials: KY
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Cao
mesh_terms: D000336:Aerosols; D010584:Bacteriophage phi X 174; D023482:Bioterrorism / Q000517:prevention & control; D004867:Equipment Design; D006813:Humidity; D016273:Occupational Exposure / Q000517:prevention & control; D011312:Pressure; D011481:Protective Clothing; D013997:Time Factors; D014777:Virus Diseases / Q000517:prevention & control
chemical_list: D000336:Aerosols