title: Triggering of drug release of particles in hair follicles.
abstract: Particulate drug delivery via hair follicles represents a promising concept, although requirements are high. This process must be realized at the desired depth and at the appropriate time, due to the fact that the particles themselves are not able to overcome the follicular skin barrier. In the present study, a novel triggering concept for the release of a model drug from the delivering particles is presented based on the application of two different particle types of the same size, where one particle type is the drug carrier, and the second one is loaded with a protease. The latter particle type is supposed to interact with the drug-carrying particles to trigger the drug release. A mixture of both particles was applied onto porcine skin samples, followed by follicular analysis. As a control, the particles were applied unaided without protease, whereas one skin area remained untreated. The investigations revealed that the protease was able to release the model drug from the delivering particles in significant depths within the hair follicle (866±62nm). Additionally, an uptake of the model drug in the sebaceous gland was observed after release providing a promising novel approach for the development of treatment strategies for different skin diseases.
authors.affiliation: Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Wing Cheung
authors.initials: WC
authors.lastname: Mak
authors.forename: Alexa
authors.initials: A
authors.lastname: Patzelt
authors.forename: Heike
authors.initials: H
authors.lastname: Richter
authors.forename: Reinhardt
authors.initials: R
authors.lastname: Renneberg
authors.forename: Kwok Kei
authors.initials: KK
authors.lastname: Lai
authors.forename: Eckart
authors.initials: E
authors.lastname: Rühl
authors.forename: Wolfram
authors.initials: W
authors.lastname: Sterry
authors.forename: Jürgen
authors.initials: J
authors.lastname: Lademann
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002119:Calcium Carbonate / Q000008:administration & dosage / Q000737:chemistry; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D018859:Hair Follicle / Q000378:metabolism*; D010316:Particle Size; D010447:Peptide Hydrolases / Q000008:administration & dosage* / Q000737:chemistry; D012627:Sebaceous Glands / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry; D012869:Skin Absorption; D013552:Swine
chemical_list: D004337:Drug Carriers; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D010447:Peptide Hydrolases; D002119:Calcium Carbonate; D016650:Fluorescein-5-isothiocyanate