title: [Sequence comparison of the hemagglutinin gene of the duck-origin H9N2 subtype avian influenza viruses].
abstract: To demonstrate the phylogenetic evolution, the molecular characteristics of the motif of HA protein cleavage site and the varieties at the receptor binding sites of the hemagglutinin gene of the duck-origin H9N2 subtype avian influenza viruses, sequence alignment and phylogenetic analysis were performed by MEGA 4.1 Neighbor-Joining method.. The results revealed that the duck-origin H9N2 AIV viruses originated from CK/BJ/1/94-like and North-Ame-like, all the duck-origin H9N2 AIV viruses from mainland China belonged to CK/BJ/1/94-like and formed multiple genotypes through complicated re-assortment, while other duck-origin H9N2 AIV, isolated from other countries in Aisa, American and European such as Korea, Japan, Alberta, Austria, Switzerland, Iran, belonged to the North-Ame-like phylogenetic lineage. The amino acids at positions 183, 190, and 226 of the receptor binding sites of North-Ame-like group isolates had highly conserved H, E and Q respectively. In contrast with duck-origin H9N2 AIV viruses isolates from mainland China, the amino acids had N at positions 183, A, T, or V at 190, L or Q at 226, which was the same as the chicken-origin H9N2 AIV from mainland China. Most newly isolated chicken-origin H9N2 AIV in Fujian Province in Southern China had L at position 226 emphasized the higher risk of cross-infection between the chicken-origin and duck-origin H9N2 AIV in China.
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China. chunhewan@126.com
authors.forename: Chun-He
authors.initials: CH
authors.lastname: Wan
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Fu
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Cheng
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Shi
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Chen
authors.forename: Chun-Xiang
authors.initials: CX
authors.lastname: Peng
authors.forename: Fang
authors.initials: F
authors.lastname: Lin
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Lin
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D002681:China; D004372:Ducks; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000235:genetics*; D053127:Influenza A Virus, H9N2 Subtype / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009980:Influenza A virus / Q000737:chemistry / Q000145:classification / Q000235:genetics; D005585:Influenza in Birds / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D016415:Sequence Alignment
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus