title: Influence of helium-neon laser irradiation on seed germination in vitro and physico-biochemical characters in seedlings of brinjal (Solanum melongena L.) var. Mattu Gulla.
abstract: In the present study, the seeds of brinjal (Solanum melongena L.) var. Mattu Gulla were irradiated with single exposure of He-Ne laser at different doses of 5-40 J cm(-2) and germinated aseptically. Thirty day old seedlings were harvested and the germination, growth, physiological and biochemical parameters were estimated and compared with un-irradiated control seedlings. A significant enhancement in growth characters were noted with respect to length, fresh and dry weight of shoots and roots. In addition, chlorophyll (a and b), carotenoid content, anthocyanin and amylases (α and β) activities were found to be altered. Significant alterations in percentage of seed germination (P < 0.001) and time to 50% germination (P < 0.001) were observed in the irradiated seeds compared with the un-irradiated controls. In conclusion, the results of the present study demonstrated that low dose (5-30 J cm(-2) ) of He-Ne laser irradiation enhanced the germination process and altered growth, by positively influencing physiological and biochemical parameters of the brinjal seedlings compared with un-irradiated control under in vitro conditions.
authors.affiliation: Division of Biotechnology, Manipal Life Sciences Centre, Manipal University, Manipal, Karnataka, India. amsamy20@gmail.com
authors.forename: Annamalai
authors.initials: A
authors.lastname: Muthusamy
authors.forename: Prathibha P
authors.initials: PP
authors.lastname: Kudwa
authors.forename: Vijendra
authors.initials: V
authors.lastname: Prabhu
authors.forename: Krishna K
authors.initials: KK
authors.lastname: Mahato
authors.forename: Vidhu Sankar
authors.initials: VS
authors.lastname: Babu
authors.forename: Mattu Radhakrishna
authors.initials: MR
authors.lastname: Rao
authors.forename: Puthiya Mandyat
authors.initials: PM
authors.lastname: Gopinath
authors.forename: Kapaettu
authors.initials: K
authors.lastname: Satyamoorthy
mesh_terms: D000681:Amylases / Q000378:metabolism; D000872:Anthocyanins / Q000096:biosynthesis; D002338:Carotenoids / Q000096:biosynthesis; D002734:Chlorophyll / Q000096:biosynthesis; D004307:Dose-Response Relationship, Radiation; D018525:Germination / Q000502:physiology / Q000528:radiation effects*; D054020:Lasers, Gas; D008027:Light; D018517:Plant Roots / Q000502:physiology / Q000528:radiation effects*; D018520:Plant Shoots / Q000502:physiology / Q000528:radiation effects*; D011829:Radiation Dosage; D036226:Seedlings / Q000502:physiology / Q000528:radiation effects*; D012639:Seeds / Q000502:physiology / Q000528:radiation effects*; D032323:Solanum melongena / Q000502:physiology / Q000528:radiation effects*
chemical_list: D000872:Anthocyanins; D002734:Chlorophyll; D002338:Carotenoids; D000681:Amylases