title: Structure and gene cluster of the O-antigen of Escherichia coli O120.
abstract: The acidic O-polysaccharide (O-antigen) of Escherichia coli O120 was isolated from the lipopolysaccharide and studied by sugar analysis and NMR spectroscopy. The following structure of the branched hexasaccharide repeating unit was established, which is unique among the known structures of bacterial polysaccharides: [formula see text] The O-antigen gene cluster of E. coli O120 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-polysaccharide structure.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: Quan
authors.initials: Q
authors.lastname: Wang
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Yanping
authors.initials: Y
authors.lastname: Gong
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D019081:O Antigens / Q000737:chemistry*
chemical_list: D019081:O Antigens