title: Polarization optical-histochemical characterization and supramolecular structure of carbohydrate fibrils.
abstract: Topo-optical staining reactions were used to investigate the structures of bacterial cellulose, insect chitosan and alginic acid from brown algae. Polysaccharide complexes, glycosaminoglycans and sulfate groups were presented and demonstrated selectively. Chitosan and alginic acid are structurally similar to glycosaminoglycans (GAGs), which are constituents of human amyloid fibrils. The staining sequences shown can be used as reliable methods for histochemistry with light and polarization microscopy. They will help to clarify the complex protein-polysaccharide structure of amyloid fibrils.
authors.affiliation: University of West Hungary, Institute of Wood and Paper Technology, Sopron, Hungary. lcsoka@fmk.nyme.hu
authors.forename: Levente
authors.initials: L
authors.lastname: Csoka
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Appel
authors.forename: Annett
authors.initials: A
authors.lastname: Eitner
authors.forename: Gustav
authors.initials: G
authors.lastname: Jirikowski
authors.forename: Josef
authors.initials: J
authors.lastname: Makovitzky
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001419:Bacteria / Q000737:chemistry; D002236:Carbohydrate Conformation; D002482:Cellulose / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006651:Histocytochemistry; D007313:Insecta / Q000737:chemistry; D008027:Light; D008859:Microscopy, Polarization; D000459:Phaeophyceae / Q000737:chemistry; D013194:Staining and Labeling
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D002482:Cellulose; D048271:Chitosan