title: Profile of trans fatty acids (FAs) including trans polyunsaturated FAs in representative fast food samples.
abstract: The content of trans fat in foods is most commonly determined by summing the levels of individual trans fatty acids (FAs), analyzed as FA methyl esters (FAME) by gas chromatography. Current Official Methods of the American Oil Chemists' Society (AOCS) enable quantitation of total trans fat in foods but were not designed for the determination of transFA isomeric compositions. In the present study, the content of trans fat in 32 representative fast food samples ranged from 0.1 to 3.1 g per serving, as determined according to AOCS Official Method Ce 1j-07. Further analysis of FAME using the 200 m SLB-IL111 ionic liquid column yielded quantitative results of total, trans, saturated, and cis unsaturated fat that were comparable to those of Method Ce 1j-07 and also allowed for the complementary determination of individual trans 18:1, trans 18:2, and trans 18:3 FA isomeric compositions under conditions suitable for routine sample analysis.
authors.affiliation: Center for Food Safety and Applied Nutrition, Office of Regulatory Science, U.S. Food and Drug Administration, 5100 Paint Branch Parkway, College Park, Maryland 20740, United States. cynthia.tyburczy@fda.hhs.gov
authors.forename: Cynthia
authors.initials: C
authors.lastname: Tyburczy
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Delmonte
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Fardin-Kia
authors.forename: Magdi M
authors.initials: MM
authors.lastname: Mossoba
authors.forename: John K G
authors.initials: JK
authors.lastname: Kramer
authors.forename: Jeanne I
authors.initials: JI
authors.lastname: Rader
mesh_terms: D057140:Fast Foods / Q000009:adverse effects / Q000032:analysis*; D005231:Fatty Acids, Unsaturated / Q000032:analysis* / Q000737:chemistry; D005410:Flame Ionization; D005513:Food Inspection / Q000379:methods* / Q000592:standards; D008396:Maryland; D012147:Restaurants; D013237:Stereoisomerism; D044242:Trans Fatty Acids / Q000032:analysis* / Q000737:chemistry
chemical_list: D005231:Fatty Acids, Unsaturated; D044242:Trans Fatty Acids