title: Simultaneous quantification of coumarins, flavonoids and limonoids in Fructus Citri Sarcodactylis by high performance liquid chromatography coupled with diode array detector.
abstract: A high performance liquid chromatography coupled with diode array detector (HPLC-DAD) method was developed for simultaneous quantification of eleven major bioactive components including six coumarins, three flavonoids and two limonoids in Fructus Citri Sarcodactylis. The analysis was performed on a Cosmosil 5 C(18)-MS-II column (4.6 mm × 250 mm, 5 μm) with water-acetonitrile gradient elution. The method was validated in terms of linearity, sensitivity, precision, stability and accuracy. It was found that the calibration curves for all analytes showed good linearity (R(2)>0.9993) within the test ranges. The overall limit of detection (LOD) and limit of quantification (LOQ) were less than 3.0 and 10.2 ng. The relative standard deviations (RSDs) for intra- and inter-day repeatability were not more than 4.99% and 4.92%, respectively. The sample was stable for at least 48 h. The spike recoveries of eleven components were 95.1-104.9%. The established method was successfully applied to determine eleven components in three samples from different locations. The results showed that the newly developed HPLC-DAD method was linear, sensitive, precise and accurate, and could be used for quality control of Fructus Citri Sarcodactylis.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine (University of Macau), Macao.
authors.forename: Jun
authors.initials: J
authors.lastname: Chu
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Li
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Yin
authors.forename: Wen-Cai
authors.initials: WC
authors.lastname: Ye
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Zhang
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D057230:Limit of Detection; D036701:Limonins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D003374:Coumarins; D005419:Flavonoids; D036701:Limonins