title: [Role of extracellular signal-regulated protein kinase 5 in the biosynthesis of follicle-stimulating hormone-stimulated progesterone in primary granulosa cells].
abstract: OBJECTIVE
  To study the role of extracellular signal-regulated protein kinase 5 (ERK5) during the biosynthesis of follicle-stimulating hormone (FSH)-mediated progesterone in primary granulosa cells.
  
  
  METHODS
  The expressions of phosphorylated and general forms of ERKS in primary granulosa cells after the treatment of FSH were detected by Western blot analysis. The subcellular localization of ERK5 was observed by confocal microscopy. The effect of ERK5 on FSH-mediated progesterone biosynthesis in primary granulosa cells was analyzed using recombinant adenovirus vectors.
  
  
  RESULTS
  ERK5 activation was induced by FSH in a time-dependent manner in primary cultured granulosa cells, although the general ERK5 protein level decreased also in a time-dependent manner. The treatment of FSH showed no remarkable effect on the subcellular distribution of endogenous ERK5, which was mainly in the cytoplasm of granulosa cells. The co-infection of Ad-caMEK5 and Ad-wtERK5 increased the progesterone production and StAR expression in primary cultured granulosa cells, whereas inhibition of ERK5 activation suppressed the FSH-stimulated progesterone production.
  
  
  CONCLUSIONS
  ERK5 may stimulate FSH-mediated progesterone production in primary cultured granulosa cells.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Gao
authors.forename: Nan
authors.initials: N
authors.lastname: Yao
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
authors.forename: Cai-Ling
authors.initials: CL
authors.lastname: Lu
authors.forename: Bo-Qing
authors.initials: BQ
authors.lastname: Yang
authors.forename: Xiao-Zhong
authors.initials: XZ
authors.lastname: Peng
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D048054:Mitogen-Activated Protein Kinase 7 / Q000378:metabolism / Q000502:physiology*; D011374:Progesterone / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011374:Progesterone; D005640:Follicle Stimulating Hormone; D048054:Mitogen-Activated Protein Kinase 7