title: Differentiation of septic and aseptic loosening by PET with both 11C-PK11195 and 18F-FDG in rat models.
abstract: OBJECTIVE
  This study aims to determine the value of PET with C-isoquinoline carboxamide (C-PK11195) and F-fluorodeoxyglucose (F-FDG) in assisting the differentiation of aseptic loosening (AL) from septic loosening (SL) in rat models.
  
  
  METHODS
  Initially, the histological profiles of SL and AL (cellular infiltration and the number of CD68 macrophage and PBR cells) were compared. Subsequently, we investigated whether C-PK11195 alone and also in combination with F-FDG increases the sensitivity and specificity of PET imaging for distinguishing SL from AL.
  
  
  RESULTS
  There were distinguishable features between the histological profiles of the SL and AL rat groups. The number of CD68/PBR cells in AL rats was significantly higher than that seen in SL rats (P<0.05). The uptake of C-PK1195 was higher in AL and lower in SL rats. The uptake of F-FDG was higher in SL and lower in AL rats.
  
  
  CONCLUSIONS
  PET with a C-PK11195 and F-FDG imaging protocol is helpful in the clinical differential diagnosis of AL from SL.
authors.affiliation: Department of Biomedical Engineering, Wayne State University, Detroit, MI 48201, USA. as7606@wayne.edu
authors.forename: Weiping
authors.initials: W
authors.lastname: Ren
authors.forename: Otto
authors.initials: O
authors.lastname: Muzik
authors.forename: Nancy
authors.initials: N
authors.lastname: Jackson
authors.forename: Basma
authors.initials: B
authors.lastname: Khoury
authors.forename: Tong
authors.initials: T
authors.lastname: Shi
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Flynn
authors.forename: Pulak
authors.initials: P
authors.lastname: Chakraborty
authors.forename: David C
authors.initials: DC
authors.lastname: Markel
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D000970:Antineoplastic Agents; D019645:Arthroplasty, Replacement, Knee / Q000009:adverse effects*; D002250:Carbon Radioisotopes; D002352:Carrier Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D019788:Fluorodeoxyglucose F18; D005549:Foreign-Body Reaction / Q000000981:diagnostic imaging / Q000209:etiology; D006614:Hindlimb / Q000000981:diagnostic imaging; D007546:Isoquinolines; D007720:Knee Prosthesis / Q000009:adverse effects*; D010865:Pilot Projects; D049268:Positron-Emission Tomography / Q000379:methods*; D011475:Prosthesis Failure; D016459:Prosthesis-Related Infections / Q000000981:diagnostic imaging* / Q000209:etiology; D019275:Radiopharmaceuticals; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000000981:diagnostic imaging* / Q000209:etiology; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000970:Antineoplastic Agents; D002250:Carbon Radioisotopes; D002352:Carrier Proteins; D019788:Fluorodeoxyglucose F18; D007546:Isoquinolines; D019275:Radiopharmaceuticals; D011963:Receptors, GABA-A; C539355:CD68 protein, rat; D000097382:CD68 Molecule; C069997:Tspo protein, rat; C037850:PK 11195