title: Isolation and characterization of a new iturinic lipopeptide, mojavensin A produced by a marine-derived bacterium Bacillus mojavensis B0621A.
abstract: Three lipopeptides were isolated by bioactivity-guided fractionation from the fermentation broth of Bacillus mojavensis B0621A. A new iturinic lipopeptide, named mojavensin A, was tentatively characterized by 1D, 2D NMR and MS spectroscopy, Marfey's method containing a novel peptide backbone of L-Asn₁, D-Tyr₂, D-Asn₃, L-Gln₄, L-Pro₅, D-Asn₆, L-Asn₇ and an anteiso-type of the saturated β-fatty acid side chain. Compound 2 and 3 were tentatively identified as iso-C16 fengycin B and anteiso-C17 fengycin B, respectively. These lipopeptides displayed dose-dependent antifungal activity against a broad spectra of phytopathogens and were weakly antagonistic to Staphylococcus aureus. Moreover, they all revealed cytotoxic activities against the human leukemia (HL-60) cell line. Mojavensin A, iso-C16 fengycin B, and anteiso-C17 fengycin B inhibited the growth of HL-60 with IC₅₀ of 100, 100 and 1.6 μM, respectively.
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
authors.forename: Zongwang
authors.initials: Z
authors.lastname: Ma
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Jiangchun
authors.initials: J
authors.lastname: Hu
authors.forename: Shujin
authors.initials: S
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001407:Bacillus / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D002470:Cell Survival / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D055666:Lipopeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D016415:Sequence Alignment; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D055666:Lipopeptides; D010456:Peptides, Cyclic