title: Screen-printed acetylcholinesterase-based biosensors for inhibitive determination of permethrin.
abstract: An amperometric assay based on acetylcholinesterase (AChE) inactivation has been developed for the monitoring of permethrin using a screen-printed three-electrode system. The enzyme AChE catalyzes the hydrolysis of acetylthiocholine to thiocholine, which can be electrochemically oxidized. The presence of permethrin inhibits the AChE activity, resulting in a lower thiocholine production and thus, a decrease in the amperometric oxidation current. Immobilization of AChE was performed by cross-linking giving a capability of detection of 8.1±0.4 μM. Repeatability and reproducibility of the developed AChE biosensor were also calculated, yielding values of 9.6% (n=4) and 5.4% (n=5), respectively related to the slopes of the calibration curves performed in the range from 6.2 up to 41 μM. The method was successfully applied to the determination of permethrin content in a commercial lice gel.
authors.affiliation: Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Pza, Misael Bañuelos s/n, 09001 Burgos, Spain. olgado@ubu.es
authors.forename: Olga
authors.initials: O
authors.lastname: Domínguez-Renedo
authors.forename: M Asunción
authors.initials: MA
authors.lastname: Alonso-Lomillo
authors.forename: Pedro
authors.initials: P
authors.lastname: Recio-Cebrián
authors.forename: M Julia
authors.initials: MJ
authors.lastname: Arcos-Martínez
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D015374:Biosensing Techniques; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000737:chemistry; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis* / Q000737:chemistry; D007306:Insecticides / Q000032:analysis* / Q000737:chemistry; D026023:Permethrin / Q000032:analysis* / Q000737:chemistry
chemical_list: D002800:Cholinesterase Inhibitors; D004785:Environmental Pollutants; D007306:Insecticides; D026023:Permethrin; D000110:Acetylcholinesterase