title: Junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and LIF.
abstract: BACKGROUND
  Junctional adhesion molecule 2 (Jam2) is a member of the JAM superfamily. JAMs are localized at intercellular contacts and participated in the assembly and maintenance of junctions, and control of cell permeability. Because Jam2 is highly expressed in the luminal epithelium on day 4 of pregnancy, this study was to determine whether Jam2 plays a role in uterine receptivity and blastocyst attachment in mouse uterus.
  
  
  RESULTS
  Jam2 is highly expressed in the uterine luminal epithelium on days 3 and 4 of pregnancy. Progesterone induces Jam2 expression in ovariectomized mice, which is blocked by progesterone antagonist RU486. Jam2 expression on day 4 of pregnancy is also inhibited by RU486 treatment. Leukemia inhibitory factor (LIF) up-regulates Jam2 protein in isolated luminal epithelium from day 4 uterus, which is blocked by S3I-201, a cell-permeable inhibitor for Stat3 phosphorylation. Under adhesion assay, recombinant Jam2 protein increases the rate of blastocyst adhesion. Both soluble recombinant Jam2 and Jam3 can reverse this process.
  
  
  CONCLUSIONS
  Jam2 is highly expressed in the luminal epithelium of receptive uterus and up-regulated by progesterone and LIF via tyrosine phosphorylation of Stat3. Jam2 may play a role in the interaction between hatched blastocyst and receptive uterus.
authors.affiliation: Department of Biology, Shantou University, Shantou, China.
authors.forename: Ren-Wei
authors.initials: RW
authors.lastname: Su
authors.forename: Bo
authors.initials: B
authors.lastname: Jia
authors.forename: Hua
authors.initials: H
authors.lastname: Ni
authors.forename: Wei
authors.initials: W
authors.lastname: Lei
authors.forename: Shun-Li
authors.initials: SL
authors.lastname: Yue
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Feng
authors.forename: Weng-Bo
authors.initials: WB
authors.lastname: Deng
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Liu
authors.forename: Zhen-Ao
authors.initials: ZA
authors.lastname: Zhao
authors.forename: Tong-Song
authors.initials: TS
authors.lastname: Wang
authors.forename: Zeng-Ming
authors.initials: ZM
authors.lastname: Yang
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000502:physiology*; D010064:Embryo Implantation; D004848:Epithelium / Q000378:metabolism; D005260:Female; D007365:Intercellular Junctions / Q000378:metabolism; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D051379:Mice; D015735:Mifepristone / Q000494:pharmacology; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D014599:Uterus / Q000378:metabolism; D062765:Junctional Adhesion Molecule B
chemical_list: D015815:Cell Adhesion Molecules; D053605:Leukemia Inhibitory Factor; D015735:Mifepristone; D011374:Progesterone; C498781:Jam2 protein, mouse; D062765:Junctional Adhesion Molecule B