title: Rapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring mass spectrometry.
abstract: Parvalbumins beta (β-PRVBs) are considered the major fish allergens. A new strategy for the rapid and direct detection of these allergens in any foodstuff is presented in this work. The proposed methodology is based on the purification of β-PRVBs by treatment with heat, the use of accelerated in-solution trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of only nineteen β-PRVB peptide biomarkers by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The present strategy allows the direct detection of the presence of fish β-PRVBs in any food product in less than 2 hours.
authors.affiliation: Institute of Molecular Systems Biology, ETH Zürich, Wolfgang-Pauli-Strasse 16, Zürich, Switzerland. carrera@imsb.biol.ethz.ch
authors.forename: Mónica
authors.initials: M
authors.lastname: Carrera
authors.forename: Benito
authors.initials: B
authors.lastname: Cañas
authors.forename: José M
authors.initials: JM
authors.lastname: Gallardo
mesh_terms: D000485:Allergens / Q000032:analysis*; D000818:Animals; D029941:Fish Proteins / Q000032:analysis*; D005399:Fishes; D005504:Food Analysis; D005512:Food Hypersensitivity; D006801:Humans; D013058:Mass Spectrometry; D010320:Parvalbumins / Q000032:analysis*; D017747:Seafood / Q000032:analysis*
chemical_list: D000485:Allergens; D029941:Fish Proteins; D010320:Parvalbumins