title: Immunocytochemical and immunohistochemical application of monoclonal antibodies against peroxisomal biogenesis factor 14.
abstract: PEX14 is an integral membrane protein essential for protein docking onto the peroxisomes and is a bi-functional protein capable of acting as a transcriptional co-repressor and a polypeptide transport modulator. Further studies showed that PEX14 is the sole peroxin that has a unique dual function in peroxisome formation and selective degradation. Its RNA transcripts are ubiquitously expressed; there is, however, no data on the expression profiles of PEX14 at the protein level due to a lack of MAbs suitable for immunohistochemical staining, thus hindering studies on its functions. In the present study, we generated one MAb that could be used in immunocytochemistry and immunohistochemistry and investigated PEX14 expression in normal and malignant human tissues. In contrast to the ubiquitous expression of its RNA transcripts, there is no PEX14 protein expression in normal human tissue, including liver, spleen, lung, rectum, brain, prostate, breast, ovary, and stomach. Protein expression of PEX14 was dramatically upregulated in some malignancies. Presented here are the first data on the expression profiles of PEX14 at the protein level, which will further our understanding of its functions.
authors.affiliation: Department of Cardiology, First Hospital of Xi'an, Xi'an, PR China.
authors.forename: Shaojuan
authors.initials: S
authors.lastname: Yu
authors.forename: Fuli
authors.initials: F
authors.lastname: Wang
authors.forename: Kui
authors.initials: K
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.forename: Manli
authors.initials: M
authors.lastname: Cheng
authors.forename: Chaojun
authors.initials: C
authors.lastname: Song
authors.forename: Boquan
authors.initials: B
authors.lastname: Jin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008565:Membrane Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D012097:Repressor Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; C455539:PEX14 protein, human; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012097:Repressor Proteins