title: Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging.
abstract: A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy.
authors.affiliation: NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12, 56127 Pisa, Italy. antonella.battisti@sns.it r.bizzarri@sns.it
authors.forename: Antonella
authors.initials: A
authors.lastname: Battisti
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Digman
authors.forename: Enrico
authors.initials: E
authors.lastname: Gratton
authors.forename: Barbara
authors.initials: B
authors.lastname: Storti
authors.forename: Fabio
authors.initials: F
authors.lastname: Beltram
authors.forename: Ranieri
authors.initials: R
authors.lastname: Bizzarri
mesh_terms: D016475:3T3 Cells; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal
chemical_list: D008164:Luminescent Proteins