title: Role of high mobility group box chromosomal protein 1 in ischemia-reperfusion injury in the rat small intestine.
abstract: BACKGROUND
  High mobility group box chromosomal protein 1 (HMGB1) has recently been shown to be an important late mediator of endotoxic shock and sepsis. The purpose of the present study was to investigate the role of HMGB1 in response to ischemia-reperfusion injury.
  
  
  METHODS
  Ischemia-reperfusion injury was induced in male Wistar rats by clamping the superior mesenteric artery for 60 min. Using this model, the serum concentrations and localization of HMGB1 were investigated. The histologic findings from reperfused intestines and the survival rates were compared between the anti-HMGB1 antibody treatment groups (group A treated with 6.0 mg/kg antibody and group B with 0.6 mg/kg antibody) and the control antibody treatment group (control group).
  
  
  RESULTS
  Serum HMGB1 concentrations increased early after reperfusion and peaked at 3 h. Immunohistochemistry for HMGB1 revealed a high degree of positive staining in the epithelial cells of the damaged villi. Anti-HMGB1 antibody treatment significantly reduced this damage (P < 0.05) and improved the 48-h survival rate (90% in group A versus 50% in the controls; P < 0.05).
  
  
  CONCLUSIONS
  These results suggest that HMGB1 plays a key role in small intestinal ischemia-reperfusion injury.
authors.affiliation: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kojima
authors.forename: Minoru
authors.initials: M
authors.lastname: Tanabe
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shinoda
authors.forename: Shingo
authors.initials: S
authors.lastname: Yamada
authors.forename: Taku
authors.initials: T
authors.lastname: Miyasho
authors.forename: Koichi
authors.initials: K
authors.lastname: Suda
authors.forename: Taizo
authors.initials: T
authors.lastname: Hibi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Obara
authors.forename: Osamu
authors.initials: O
authors.lastname: Itano
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kawachi
authors.forename: Masaki
authors.initials: M
authors.lastname: Kitajima
authors.forename: Ikuro
authors.initials: I
authors.lastname: Maruyama
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kitagawa
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D004195:Disease Models, Animal; D024243:HMGB1 Protein / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism* / Q000473:pathology; D008297:Male; D017538:Mesenteric Artery, Superior; D051381:Rats; D011920:Rats, Inbred WF; D015427:Reperfusion Injury / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D013525:Surgical Instruments; D015996:Survival Rate
chemical_list: D000906:Antibodies; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat