title: Investigation of the binding behaviors of isonucleoside-incorporated oligonucleotides with complementary sequences.
abstract: Oligonucleotides consisting of isonucleoside 2',5'-anhydro-3'-nucleobase-D-mannitol incorporated in 1'→4' linkage mode were synthesized. Their binding behaviors with complementary sequences were investigated via thermal denaturation and CD spectra. 6' O-methyl-2',5'-anhydro-3'-(thymin-1-yl)-D-mannitol incorporated oligonucleotide was also synthesized to investigate the effect of hydroxy groups of isonucleosides on duplex formation. The results showed that the 6'-OH free isonucleosidemodified oligonucleotide was able to form a B-like duplex with 3'→5' complementary native oligodeoxynucleotide in the 1'→4' direction. The free hydroxy group in the isonucleoside made a significant contribution to the affinity of the modified oligonucleotide to the complementary sequence, which was confirmed by molecular dynamics simulation.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Guan
authors.forename: H W
authors.initials: HW
authors.lastname: Jin
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Yang
authors.forename: L R
authors.initials: LR
authors.lastname: Zhang
authors.forename: L H
authors.initials: LH
authors.lastname: Zhang