title: Imageable fluorescent metastasis resulting in transgenic GFP mice orthotopically implanted with human-patient primary pancreatic cancer specimens.
abstract: Tumors from pancreatic cancer patients were established in NOD/SCID mice immediately after surgery and subsequently passaged orthotopically in transgenic nude mice ubiquitously expressing green fluorescent protein (GFP). The primary patient tumors acquired GFP-expressing stroma. Subsequent liver metastases, and disseminated peritoneal metastases maintained the stroma from the primary tumor, and possibly recruited additional GFP-expressing stroma, resulting in their very bright fluorescence. The GFP-expressing stroma included cancer-associated fibroblasts and tumor-associated macrophages in both the primary and metastatic tumors. This imageable model of metastasis from a patient-tumor is an important advance over patient "tumorgraft" models currently in use, which are implanted subcutaneously, do not metastasize and are not imageable. The new imageable model of patient pancreatic cancer metastasis provides unique opportunities to identify current and novel antimetastatic therapeutics for individual patients.
authors.affiliation: Anti Cancer, Inc., San Diego, CA 92111, USA.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suetsugu
authors.forename: Matthew
authors.initials: M
authors.lastname: Katz
authors.forename: Jason
authors.initials: J
authors.lastname: Fleming
authors.forename: Mark
authors.initials: M
authors.lastname: Truty
authors.forename: Ryan
authors.initials: R
authors.lastname: Thomas
authors.forename: Shigetoyo
authors.initials: S
authors.lastname: Saji
authors.forename: Hisataka
authors.initials: H
authors.lastname: Moriwaki
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000473:pathology*
chemical_list: D049452:Green Fluorescent Proteins