title: Simultaneous detection of seven enteric viruses associated with acute gastroenteritis by a multiplexed Luminex-based assay.
abstract: Rapid and broad diagnostic methods are needed for the identification of viral agents of gastroenteritis. In this study, we used Luminex xMAP technology to develop a multiplexed assay for the simultaneous identification of major enteric viral pathogens, including rotavirus A (RVA), noroviruses (NoVs) (including genogroups GI and GII), sapoviruses (SaV), human astrovirus (HAstV), enteric adenoviruses (EAds), and human bocavirus 2 (HBoV2). The analytical sensitivity allowed detection of 10(3) (EAds, HBoV2, and RVA) and 10(4) (NoV GI and GII, SaV, and HAstV) copies per reaction mixture. Compared to conventional PCR, the Luminex-based assay yielded greater than 75% sensitivity and 97% specificity for each virus, and the kappa correlation for detection of all viruses ranged from 0.75 to 1.00. In conclusion, this multiplexed Luminex-based assay provides a potentially rapid, high-throughput, and maneuverable diagnostic tool for major viral pathogens associated with gastroenteritis.
authors.affiliation: State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zi-qian
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.forename: Jie-mei
authors.initials: JM
authors.lastname: Yu
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Shu-xian
authors.initials: SX
authors.lastname: Cui
authors.forename: Xiang-yu
authors.initials: XY
authors.lastname: Kong
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Hui-ying
authors.initials: HY
authors.lastname: Li
authors.forename: Wei-xia
authors.initials: WX
authors.lastname: Cheng
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Ma
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D002648:Child; D002675:Child, Preschool; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology; D014780:Viruses / Q000145:classification / Q000302:isolation & purification*