title: Lack of association between herpes simplex virus type 2 infection and cervical cancer--Taq Man realtime PCR assay findings.
abstract: BACKGROUND
  About one third of the human population suffer cancer during their lifetime and more than 20% of total morbidity is related to neoplasia. Cervical cancer is generally the most common cancer in developing countries and the second most common in women globally. The role of human papilloma viruses viruses in its induction is clear. However, the involvement of herpes simplex virus type 2 (HSV-2) is controversial. Therefore a survey was conducted of the prevalence of HSV-2 in patients with cervical cancer and also healthy people with sensitive and quantitative Taq Man real-time PCR assay.
  
  
  METHODS
  Seventy six formaldehyde fixed paraffin embedded tissue specimens from patients with histologically proven history of cervical cancer as well as 150 control blocks were sectioned for deparaffinization and DNA extraction.
  
  
  RESULTS
  There was no HSV-2 DNA in our patient specimens but four control samples were positive, all with a history of hysterectomy.
  
  
  CONCLUSIONS
  Considering the absence of any positive viral HSV-2 DNA in our patients and also the presence of four positive specimens among our controls, we did not find any relationship between the presence of HSV-2 DNA and cervical cancer.
authors.affiliation: Cell and Molecular Research Center, Qazvin University of Medical Sciences, Qavzin, Iran. tnaserpour@qums.ac.ir
authors.forename: Taghi Naserpour
authors.initials: TN
authors.lastname: Farivar
authors.forename: Pouran
authors.initials: P
authors.lastname: Johari
authors.forename: Shilan
authors.initials: S
authors.lastname: Shafei
authors.forename: Reza
authors.initials: R
authors.lastname: Najafipour
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006561:Herpes Simplex / Q000150:complications* / Q000453:epidemiology / Q000821:virology; D018258:Herpesvirus 2, Human / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000453:epidemiology / Q000209:etiology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral