title: Light-emitting-diode-induced fluorescence detection of fluorescent dyes for capillary electrophoresis microchip with cross-polarization method.
abstract: A cross-polarization scheme is presented to filter out the excitation light from the emission spectrum of fluorescent dyes using green light emitting diodes as a light source and a linear charge coupled device as an intensity detector. The excitation light was linearly polarized and was then used to illuminate the fluorescent dyes in the microchannels of a capillary electrophoresis microchip. The detector was shielded by the second polarizer, oriented perpendicular to the excitation light. The fluorescent signals from Rhodamine B dyes were measured in a dilution series with resulting emission signals and four different concentrations of fluorescent dyes were detected simultaneously with the same excitation source and detector. A limit-of-detection of 1 μM was demonstrated for Rhodamine B dye under the optimal conditions.
authors.affiliation: School of Electronic Science and Technology, Dalian University of Technology, Dalian, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Yang
authors.forename: Weiping
authors.initials: W
authors.lastname: Yan
authors.forename: Zhihuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Hongfeng
authors.initials: H
authors.lastname: Lv
mesh_terms: D046209:Electrophoresis, Microchip; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis*; D005898:Glass; D057230:Limit of Detection; D012235:Rhodamines / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; C029773:rhodamine B