title: [Determination of content changes of three diester-diterpenoid alkaloids in compatibility of radix aconiti laterlis preparata and Rhizoma pinelliae, Fructus trichosanthis, Bulbus fritillariae, Radix ampelopsis, Rhizoma bletillae by HPLC].
abstract: OBJECTIVE
  To analyze the three diester-alkaloids content in the decoctions before and after Radix aconiti lateralis preparata combined with Rhizoma pinelliae, Fructus trichosanthis, Bulbus fritillariae, Radix ampelopsis, Rhizoma bletillae, respectively.
  
  
  METHODS
  HPLC analysis was performed on a Agilent Extend C18 column,eluted with a mobile phase consisted of acetonitrile/methanol - 35 mmol/L ammonium acetate and gradient elution,with a flow rate of 0.6 ml/min and the detection wavelength of 235 nm.
  
  
  RESULTS
  The contents of three diester-alkaloids in the co-decoctions of Radix aconiti laterlis preparata combined with Rhizoma pinelliae, Fructus trichosanthis, Bulbus fritillariae, Rhizoma bletillae were more than that of single Radix aconiti laterlis preparata decoction, expect the Bulbus fritillariae cirrhosae,Radix ampelopsis and Rhizoma pinelliae praeparatum. From the result,we can found that the content of three diester-alkaloids in decoctions was correlated with the decoction pH.
  
  
  CONCLUSIONS
  The pH of decoction is one of the most important factors to relate the three diester-alkaloids content in the decoctions.
authors.affiliation: Changchun University of Traditional Chinese Medicine, Changchun 130117, China. dongxin.16@163.com
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.forename: Shu-Min
authors.initials: SM
authors.lastname: Wang
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Li
authors.forename: Feng-Rui
authors.initials: FR
authors.lastname: Song
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Liu
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D031410:Fritillaria / Q000737:chemistry; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D029595:Orchidaceae / Q000737:chemistry; D030021:Pinellia / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D029778:Trichosanthes / Q000737:chemistry; D027844:Vitaceae / Q000737:chemistry
chemical_list: D000470:Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C058309:hypaconitine; C019470:mesaconitine; D000157:Aconitine