title: The effect of dietary zinc--and polyphenols intake on DMBA-induced mammary tumorigenesis in rats.
abstract: BACKGROUND
  The aim of the study was to investigate the effect of dietary supplementation with zinc and polyphenol compounds, i.e. resveratrol and genistein, on the effectiveness of chemically induced mammary cancer and the changes in the content of selected elements (Zn, Cu, Mg, Fe, Ca) in tumors as compared with normal tissue of the mammary gland.
  
  
  METHODS
  Female Sprague-Dawley rats were divided into study groups which, apart from the standard diet and DMBA (7,12-dimethyl-1,2- benz[a]anthracene), were treated with zinc ions (Zn) or zinc ions + resveratrol (Zn + resveratrol) or zinc ions + genistein (Zn + genistein) via gavage for a period from 40 days until 20 weeks of age. The ICP-OES (inductively coupled plasma optical emission spectrometry) technique was used to analyze the following elements: magnesium, iron, zinc and calcium. Copper content in samples was estimated in an atomic absorption spectrophotometer.
  
  
  RESULTS
  Regardless of the diet (standard; Zn; Zn + resveratrol; Zn + genistein), DMBA-induced breast carcinogenesis was not inhibited. On the contrary, in the Zn + resveratrol supplemented group, tumorigenesis developed at a considerably faster rate. On the basis of quantitative analysis of selected elements we found--irrespectively of the diet applied--great accumulation of copper and iron, which are strongly prooxidative, with a simultaneous considerable decrease of the magnesium content in DMBA-induced mammary tumors. The combination of zinc supplementation with resveratrol resulted in particularly large differences in the amount of the investigated elements in tumors as compared with their content in normal tissue.
  
  
  CONCLUSIONS
  Diet supplementation with zinc and polyphenol compounds, i.e. resveratrol and genistein had no effect on the decreased copper level in tumor tissue and inhibited mammary carcinogenesis in the rat. Irrespectively of the applied diet, the development of the neoplastic process in rats resulted in changes of the iron and magnesium content in the cancerous tissue in comparison with the healthy mammary tissue. The application of combined diet supplementation with zinc ions and resveratrol considerably promoted the rate of carcinogenesis and increased the number of DMBA-induced mammary tumors.
authors.affiliation: Department of Bromatology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, Warsaw 02-097, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Bobrowska-Korczak
authors.forename: Dorota
authors.initials: D
authors.lastname: Skrajnowska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Tokarz
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002273:Carcinogens; D019587:Dietary Supplements; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D015674:Mammary Neoplasms, Animal / Q000151:congenital* / Q000178:diet therapy; D059808:Polyphenols / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D015032:Zinc / Q000494:pharmacology*
chemical_list: D016588:Anticarcinogenic Agents; D016587:Antimutagenic Agents; D002273:Carcinogens; D059808:Polyphenols; D013267:Stilbenes; D015127:9,10-Dimethyl-1,2-benzanthracene; D019833:Genistein; D015032:Zinc; D000077185:Resveratrol