title: Hepatitis B virus X protein blocks filamentous actin bundles by interaction with eukaryotic translation elongat ion factor 1 alpha 1.
abstract: Hepatitis B virus (HBV)-encoded X protein (HBx protein) is a multi-functional regulatory protein. It functions by protein-protein interaction and plays a pivotal role in the pathogenesis of HBV-related diseases. However, the partners in hepatocytes interacting with HBx protein are far from understood fully. In this study, immunoprecipitation was employed to screen for binding partners for the HBx protein from huh-7 hepatoma cells infected with recombinant adenovirus expressing HBx protein, and five cellular proteins including eukaryotic translation elongation factor 1 alpha 1 (eEF1A1), were identified. The interaction between HBx protein and eEF1A1 was confirmed further using a GST pull-down assay and co-immunoprecipitation, respectively. In Huh-7 hepatoma cells, the HBx protein inhibits dimer formation of eEF1A1, hence blocks filamentous actin bundling. These findings provide new insights into the molecular mechanisms involved in the functions of the HBx protein.
authors.affiliation: Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Research Center of Molecular Medicine, Fujian Medical University, Fuzhou City, China.
authors.forename: Wan-Song
authors.initials: WS
authors.lastname: Lin
authors.forename: Bo-Yan
authors.initials: BY
authors.lastname: Jiao
authors.forename: Yun-Li
authors.initials: YL
authors.lastname: Wu
authors.forename: Wan-Nan
authors.initials: WN
authors.lastname: Chen
authors.forename: Xu
authors.initials: X
authors.lastname: Lin
mesh_terms: D000199:Actins / Q000037:antagonists & inhibitors*; D000256:Adenoviridae / Q000235:genetics; D002460:Cell Line; D005822:Genetic Vectors; D006515:Hepatitis B virus / Q000472:pathogenicity*; D022781:Hepatocytes / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D011485:Protein Binding; D055503:Protein Multimerization; D015534:Trans-Activators / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D000199:Actins; C094814:EEF1A1 protein, human; D020648:Peptide Elongation Factor 1; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein