title: Antibody response to Streptococcus pneumoniae proteins PhtD, LytB, PcpA, PhtE and Ply after nasopharyngeal colonization and acute otitis media in children.
abstract: We prospectively compared serum antibody levels of 5 Streptococcus pneumoniae (Spn) proteins: PcpA PhtD, PhtE Ply and LytB associated with nasopharyngeal (NP) colonization and acute otitis media (AOM) infection in a cohort of 6-30 mo old children. Antigen-specific antibody titers were determined by ELISA. A total of 731 visits among 168 children were studied. There were 301 Spn NP colonization episodes documented in 109 (65%) children and 42 Spn AOM episodes in 34 (20%) children. IgG antibody titers to the 5 proteins were significantly different among children over time (p < 0.001), with a rank order as follows: PcpA > PhtE = PhtD > Ply > LytB Characterization of IgG and IgM acute and convalescent serum antibody levels of Spn AOM infection showed the kinetics of the response differed among children, with the same rank order of antibody levels over time. Individual data showed that some children responded to AOM with an antibody increase to one or more of these Spn proteins but some children failed to respond. We conclude that antibody levels to Spn proteins PcpA PhtD, PhtE, Ply and LytB, all rise over time in children age 6 to 30 mo following natural exposure to Spn after NP colonization and AOM; however, there were significant differences in quantity of antibody elicited among these potential vaccine antigens.
authors.affiliation: Rochester General Hospital Research Institute, Rochester, NY, USA. michael.pichichero@rochestergeneral.org
authors.forename: Michael E
authors.initials: ME
authors.lastname: Pichichero
authors.forename: Ravinder
authors.initials: R
authors.lastname: Kaur
authors.forename: Janet R
authors.initials: JR
authors.lastname: Casey
authors.forename: Qingfu
authors.initials: Q
authors.lastname: Xu
authors.forename: Anthony
authors.initials: A
authors.lastname: Almudevar
authors.forename: Martina
authors.initials: M
authors.lastname: Ochs
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology*; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000276:immunology*; D009305:Nasopharynx / Q000382:microbiology*; D010033:Otitis Media / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000276:immunology*; D013301:Streptolysins / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C113653:PcpA protein, Streptococcus; D013301:Streptolysins; C032290:plY protein, Streptococcus pneumoniae; C119964:LytB protein, Streptococcus; D009238:N-Acetylmuramoyl-L-alanine Amidase