title: [The preparation of paeonol transdermal delivery systems based on the microemulsion-based gels and its pharmacokinetics characters].
abstract: Investigation of the pharmacokinetics of paeonol microemulsion, microemulsion-based gels and marketed paeonol ointments by the skin-blood synchronous microdialysis coupled with LC/MS is reported in this study. The microdialysis systems were established by linear probes and concentric circles probes. In vivo recovery of paeonol in skin is (69.7 +/- 4.8) % and in blood is (51.6 +/- 7.2)%. The paeonol microemulsion, microemulsion-based gels and marketed paeonol ointments were administered to rats. PBS (pH 7.4) served as perfused solution. The perfusion rate was 5 microL x mL(-1) and the microdialysis samples were collected every 20 min intervals. The paeonol concentration in perfused solution was determined by LC/MS. The results showed that paeonol microemulsion and microemulsion-based gels significantly raised the drug concentrations in skin more than that of paeonol ointments. The paeonol microemulsion-based gels has similar bioavailability as the paeonol ointments in blood, but its blood drug concentrations were steadier. The paeonol microemulsion-based gels may be developed into a new preparation for dermis eczema. The skin-blood synchronous microdialysis technique proved to be a new method for the pharmacokinetics study of transdermal delivery systems.
authors.affiliation: Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Ji-Yong
authors.initials: JY
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Hu
authors.forename: Zheng-Tao
authors.initials: ZT
authors.lastname: Wang
authors.forename: Kai-Xian
authors.initials: KX
authors.lastname: Chen
mesh_terms: D000098:Acetophenones / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D000279:Administration, Cutaneous; D000818:Animals; D001682:Biological Availability; D002853:Chromatography, Liquid; D016503:Drug Delivery Systems; D004655:Emulsions; D005782:Gels; D008297:Male; D013058:Mass Spectrometry; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption
chemical_list: D000098:Acetophenones; D004655:Emulsions; D005782:Gels; C013638:paeonol