title: Rapid analysis of caffeine in "smart drugs" and "energy drinks" by microemulsion electrokinetic chromatography (MEEKC).
abstract: A novel method based on microemulsion electrokinetic chromatography (MEEKC) with diode array detection (DAD) for rapid determination of caffeine in commercial and clandestine stimulants, known as "energy drinks" and "smart drugs", is described. Separations were carried out in 50 cm × 50 μm (ID) uncoated fused silica capillaries. The optimized buffer electrolyte was composed of 8.85 mM sodium tetraborate pH 9.5, SDS 3.3% (w/v), n-hexane 1.5% (v/v) and 1-butanol 6.6% (v/v). Separations were performed at a voltage of 20 kV. Sample injection conditions were 0.5 psi, 3 s. Diprofilline was used as internal standard. The determination of the analytes was based on the UV signal recorded at 275 nm, corresponding to the maximum wavelength of absorbance of caffeine, whereas peak identification and purity check was performed on the basis of the acquisition of UV radiation between 200 and 400 nm wavelengths. Under the described conditions, the separation of the compounds was achieved in 6 min without any interference from the matrix. Linearity was assessed within a caffeine concentration range from 5 to 100 μg/mL. The intra-day and inter-day precision values were below 0.37% for migration times and below 9.86% for peak areas. The present MEEKC method was successfully applied to the direct determination of caffeine in smart drugs and energy drinks.
authors.affiliation: Department of Public Health and Community Medicine, Unit of Forensic Medicine, University of Verona, Verona, Italy.
authors.forename: Eloisa
authors.initials: E
authors.lastname: Liotta
authors.forename: Rossella
authors.initials: R
authors.lastname: Gottardo
authors.forename: Catia
authors.initials: C
authors.lastname: Seri
authors.forename: Claudia
authors.initials: C
authors.lastname: Rimondo
authors.forename: Ivan
authors.initials: I
authors.lastname: Miksik
authors.forename: Giovanni
authors.initials: G
authors.lastname: Serpelloni
authors.forename: Franco
authors.initials: F
authors.lastname: Tagliaro