title: First isolation of Mycobacterium kyorinense from clinical specimens in Brazil.
abstract: In this article, the first isolation of Mycobacterium kyorinense specimens in Brazil is described. M. kyorinense is a recently identified species, with a few strains reported only in Japan. The Brazilian isolates were initially identified as Mycobacterium celatum by PCR restriction enzyme pattern analysis (PRA) with hsp65. However, biochemical tests indicated the same profile of M. kyorinense and distinguished them from M. celatum and Mycobacterium branderi. The sequencing of the hsp65, rpoB, and 16S rRNA genes allowed the accurate identification of isolates as M. kyorinense.
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz, Rio de Janeiro, Brazil.
authors.forename: Carlos Eduardo Dias
authors.initials: CE
authors.lastname: Campos
authors.forename: Paulo Cesar de Souza
authors.initials: PC
authors.lastname: Caldas
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ohnishi
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Kouki
authors.initials: K
authors.lastname: Ohtsuka
authors.forename: Satsuki
authors.initials: S
authors.lastname: Matsushima
authors.forename: Nicole Victor
authors.initials: NV
authors.lastname: Ferreira
authors.forename: Mariza Villas Boas
authors.initials: MV
authors.lastname: da Silva
authors.forename: Paulo
authors.initials: P
authors.lastname: Redner
authors.forename: Luciana Distásio
authors.initials: LD
authors.lastname: de Carvalho
authors.forename: Reginalda Ferreira de Melo
authors.initials: RF
authors.lastname: Medeiros
authors.forename: Jacyr Antônio
authors.initials: JA
authors.lastname: Abbud Filho
authors.forename: Fátima Cristina Onofre Fandinho
authors.initials: FC
authors.lastname: Montes
authors.forename: Teca Calcagno
authors.initials: TC
authors.lastname: Galvão
authors.forename: Jesus Pais
authors.initials: JP
authors.lastname: Ramos
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001938:Brazil; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis* / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit