title: Therapeutic potentials of neural stem cells treated with fluoxetine in Alzheimer's disease.
abstract: Recent studies have proposed that chronic treatment with antidepressants increases neurogenesis in the adult hippocampus. However, the effect of antidepressants on fetal neural stem cells (NSCs) has not been well defined. Our study shows the dose-dependent effects of fluoxetine on the proliferation and neural differentiation of NSCs. Fluoxetine, even at nanomolar concentrations, stimulated proliferation of NSCs and increased the number of βIII-tubulin (Tuj 1)- and neural nucleus marker (NeuN)-positive cells, but not glial fibrillary acidic protein (GFAP)-positive cells. These results suggest that fluoxetine can enhance neuronal differentiation. In addition, fluoxetine has protective effects against cell death induced by oligomeric amyloid beta (Aβ(42)) peptides. Taken together, these results clearly show that fluoxetine promotes both the proliferation and neuronal differentiation of NSCs and exerts protective effects against Aβ(42)-induced cytotoxicities in NSCs, which suggest that the use of fluoxetine is applicable for cell therapy for various neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases by its actions in NSCs.
authors.affiliation: Department of Pharmacology, College of Medicine and Neuroscience Research Institute, Medical Research Center, Seoul National University, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
authors.forename: Keun-A
authors.initials: KA
authors.lastname: Chang
authors.forename: Jeong A
authors.initials: JA
authors.lastname: Kim
authors.forename: Saeromi
authors.initials: S
authors.lastname: Kim
authors.forename: Yuyoung
authors.initials: Y
authors.lastname: Joo
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Shin
authors.forename: Seonghan
authors.initials: S
authors.lastname: Kim
authors.forename: Hye-Sun
authors.initials: HS
authors.lastname: Kim
authors.forename: Yoo-Hun
authors.initials: YH
authors.lastname: Suh
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy* / Q000628:therapy; D005473:Fluoxetine / Q000627:therapeutic use*; D006801:Humans; D058953:Neural Stem Cells / Q000187:drug effects*; D017367:Selective Serotonin Reuptake Inhibitors / Q000627:therapeutic use*
chemical_list: D005473:Fluoxetine; D017367:Selective Serotonin Reuptake Inhibitors