title: Novel microRNAs encoded by duck enteritis virus.
abstract: Duck enteritis virus (DEV) is an important herpesvirus pathogen associated with acute, highly contagious lethal disease in waterfowls. Using a deep sequencing approach on RNA from infected chicken embryo fibroblast cultures, we identified several novel DEV-encoded micro (mi)RNAs. Unlike most mardivirus-encoded miRNAs, DEV-encoded miRNAs mapped mostly to the unique long region of the genome. The precursors of DEV miR-D18 and miR-D19 overlapped with each other, suggesting similarities to miRNA-offset RNAs, although only the DEV-miR-D18-3p was functional in reporter assays. Identification of these novel miRNAs will add to the growing list of virus-encoded miRNAs enabling the exploration of their roles in pathogenesis.
authors.affiliation: Viral Oncogenesis Group, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK.
authors.forename: Yongxiu
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Viral Oncogenesis Group, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK.
authors.forename: Lorraine P
authors.initials: LP
authors.lastname: Smith
authors.affiliation: Viral Oncogenesis Group, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Petherbridge
authors.affiliation: Ark-Genomics, The Roslin Institute, R(D)SVS, University of Edinburgh, Division of Genetics and Genomics, Easter Bush, Midlothian EH25 9RG, UK.
authors.forename: Mick
authors.initials: M
authors.lastname: Watson
authors.affiliation: Viral Oncogenesis Group, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK.
authors.forename: Venugopal
authors.initials: V
authors.lastname: Nair
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D005347:Fibroblasts / Q000821:virology; D059014:High-Throughput Nucleotide Sequencing; D025922:Mardivirus / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012367:RNA, Viral