title: [Screening for serum specific biomarkers in patients with primary Sjögren's syndrome and interstitial lung disease using proteomic fingerprint techniques].
abstract: OBJECTIVE
  To detect the serum protein biomarkers and establish a diagnostic model for primary Sjögren's syndrome (pSS) with interstitial lung disease (ILD).
  
  
  METHODS
  Serum samples from 69 patients with pSS were prepared with WCX magnetic beads, and analyzed on PBS II-C mass spectrometer reader. Biomarker Wizard software was used to detect protein peaks and potential difference between the patients with pSS-ILD and with non-ILD. The model was developed by Biomarker Patterns software.
  
  
  RESULTS
  Totally 7 discriminative mass-to-charge (m/z) ratios were identified to be related with pSS-ILD (P<0.05). Among these, the m/z peaks at 3 778.3, 3 318.3 and 2 236.6 were used to construct a diagnostic model. The sensitivity and specificity of the model were 93.1% and 87.5%, respectively. In a testing set, the sensitivity and specificity of the model were 84.0% and 85.7%, respectively.
  
  
  CONCLUSIONS
  The potential protein biomarkers for pSS-ILD are discovered in the serum by MALDI-TOF-MS combined with WCX magnetic beads. The diagnostic pattern combining 3 778.3, 3 318.3 and 2 236.6 m/z protein peaks can discriminate pSS-ILD and non-ILD.
authors.affiliation: Department of Rheumatology and Immunology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Yu-hui
authors.initials: YH
authors.lastname: Li
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Sun
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.forename: Ru-lin
authors.initials: RL
authors.lastname: Jia
authors.forename: Dong-yue
authors.initials: DY
authors.lastname: Yang
authors.forename: Xue-wu
authors.initials: XW
authors.lastname: Zhang
authors.forename: Zhan-guo
authors.initials: ZG
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D017563:Lung Diseases, Interstitial / Q000097:blood* / Q000150:complications; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D012859:Sjogren's Syndrome / Q000097:blood* / Q000150:complications; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers