title: [Mechanism of Dahuang Zhechong pill against atherosclerosis induced by balloon angioplasty in rabbits].
abstract: OBJECTIVE
  To study the mechanism of Dahuang Zhechong pill (DHZCP) against atherosclerosis induced by balloon angioplasty in rabbits.
  
  
  METHODS
  Atherosclerosis model was established by the combination of balloon angioplasty-induced endothelial injury and high cholesterol feeding in rabbit. Male New Zealand rabbits were divided into six groups randomly: normal control, sham, model, positive control and two doses of DHZCP-treated groups. Rabbits in DHZCP-treated groups were intragastrically administered 0.9 and 1.8 g/kg DHZCP for 60 days respectively,and rabbits in positive control group were given 0.5 g/kg Danshen. MDA, NO levels and SOD activity in serum, and MPO activity in the vascular wall were determined with spectrophotometry. Expressions of proliferating cell nuclear antigen (PCNA) and BCL-2 in the vascular wall were detected by SP immuohistochemical technique.
  
  
  RESULTS
  Compared with the model group, DHZCP significantly reduced serum MDA level and MPO activity in the vascular wall, increased serum NO level and SOD activity,and inhibited PCNA and BCL-2 expressions in the vascular wall.
  
  
  CONCLUSIONS
  DHZCP inhibits the formation and development of atherosclerosis through anti-oxidative action, protecting endothelium from injury,inhibiting proliferation and promoting apoptosis of vascular smooth muscle cells.
authors.affiliation: Medical School of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Chun-Jie
authors.initials: CJ
authors.lastname: Han
authors.forename: Jun-Tian
authors.initials: JT
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Pang
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Mao
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism / Q000473:pathology; D017209:Apoptosis / Q000187:drug effects; D050197:Atherosclerosis / Q000097:blood / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D049109:Cell Proliferation / Q000187:drug effects*; D003058:Cockroaches / Q000737:chemistry; D004195:Disease Models, Animal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D007150:Immunohistochemistry; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011817:Rabbits; D011897:Random Allocation; D012250:Rheum / Q000737:chemistry; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: C521634:Dahuang Zhechong pill; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D018809:Proliferating Cell Nuclear Antigen; D008315:Malondialdehyde; D013482:Superoxide Dismutase