title: Improved identification of agonist-mediated Gα(i)-specific human G-protein-coupled receptor signaling in yeast cells by flow cytometry.
abstract: Flow cytometry enables comparative quantification, population analysis, and high-throughput screening of agonist-mediated G-protein-coupled receptor (GPCR) signaling in genetically engineered yeasts. By using flow cytometry, we found that transformation of yeast cells with a low plasmid number is critical both for the construction of large screening libraries and for stable signal transmission in cell ensembles. Based on these findings, we constructed an engineered yeast strain for the improved identification of signal promotion by Gα(i)-specific human GPCRs using flow cytometry.
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, Nada, Kobe 657-8501, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ishii
authors.forename: Miyuki
authors.initials: M
authors.lastname: Moriguchi
authors.forename: Kiyotaka Y
authors.initials: KY
authors.lastname: Hara
authors.forename: Seiji
authors.initials: S
authors.lastname: Shibasaki
authors.forename: Hideki
authors.initials: H
authors.lastname: Fukuda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D004791:Enzyme Inhibitors / Q000032:analysis*; D005434:Flow Cytometry; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000819:agonists* / Q000378:metabolism; D006801:Humans; D010957:Plasmids / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D017481:Receptors, Somatostatin / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D004791:Enzyme Inhibitors; D043562:Receptors, G-Protein-Coupled; D017481:Receptors, Somatostatin; C094573:somatostatin receptor 5; D019206:GTP-Binding Protein alpha Subunits, Gi-Go