title: Different agonists recruit different stromal interaction molecule proteins to support cytoplasmic Ca2+ oscillations and gene expression.
abstract: Stimulation of cells with physiological concentrations of calcium-mobilizing agonists often results in the generation of repetitive cytoplasmic Ca(2+) oscillations. Although oscillations arise from regenerative Ca(2+) release, they are sustained by store-operated Ca(2+) entry through Ca(2+) release-activated Ca(2+) (CRAC) channels. Here, we show that following stimulation of cysteinyl leukotriene type I receptors in rat basophilic leukemia (RBL)-1 cells, large amplitude Ca(2+) oscillations, CRAC channel activity, and downstream Ca(2+)-dependent nuclear factor of activated T cells (NFAT)-driven gene expression are all exclusively maintained by the endoplasmic reticulum Ca(2+) sensor stromal interaction molecule (STIM) 1. However, stimulation of tyrosine kinase-coupled FCεRI receptors evoked Ca(2+) oscillations and NFAT-dependent gene expression through recruitment of both STIM2 and STIM1. We conclude that different agonists activate different STIM proteins to sustain Ca(2+) signals and downstream responses.
authors.affiliation: Department of Physiology, Anatomy and Genetics,  University of Oxford, Oxford OX1 3PT,  United Kingdom.
authors.forename: Pulak
authors.initials: P
authors.lastname: Kar
authors.forename: Daniel
authors.initials: D
authors.lastname: Bakowski
authors.forename: Joseph
authors.initials: J
authors.lastname: Di Capite
authors.forename: Charmaine
authors.initials: C
authors.lastname: Nelson
authors.forename: Anant B
authors.initials: AB
authors.lastname: Parekh
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects* / Q000235:genetics*; D002135:Calcium-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000187:drug effects; D055785:Gene Knockdown Techniques; D017997:Leukotriene C4 / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000378:metabolism; D051381:Rats; D018077:Receptors, Leukotriene / Q000378:metabolism; D000071737:Stromal Interaction Molecule 1; D000071738:Stromal Interaction Molecule 2
chemical_list: D015220:Calcium Channels; D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D050778:NFATC Transcription Factors; D018077:Receptors, Leukotriene; C560435:STIM2 protein, rat; C518857:Stim1 protein, rat; D000071737:Stromal Interaction Molecule 1; D000071738:Stromal Interaction Molecule 2; D017997:Leukotriene C4; C083169:leukotriene D4 receptor