title: Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.).
abstract: Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5-50 μg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 μM ONOO(-) or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.
authors.affiliation: Department of Biophysics, University of Białystok, Świerkowa 20B, 15-950, Białystok, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Olchowik
authors.forename: Karol
authors.initials: K
authors.lastname: Lotkowski
authors.forename: Saidmukhtar
authors.initials: S
authors.lastname: Mavlyanov
authors.forename: Nodira
authors.initials: N
authors.lastname: Abdullajanova
authors.forename: Maksim
authors.initials: M
authors.lastname: Ionov
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
authors.forename: Maria
authors.initials: M
authors.lastname: Zamaraeva
mesh_terms: D000818:Animals; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D005978:Glutathione / Q000097:blood; D056604:Grape Seed Extract / Q000737:chemistry / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D007038:Hypotonic Solutions / Q000737:chemistry; D008706:Methemoglobin / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects*; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology; D029045:Rhus / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry / Q000494:pharmacology; D013552:Swine; D013634:Tannins / Q000494:pharmacology; D027843:Vitis / Q000737:chemistry
chemical_list: D056604:Grape Seed Extract; D007038:Hypotonic Solutions; D059808:Polyphenols; D020011:Protective Agents; D013634:Tannins; D012965:Sodium Chloride; D008706:Methemoglobin; D005978:Glutathione