title: Concentration and chain length of polyethylene glycol in islet isolation solution: evaluation in a pancreatic islet transplantation model.
abstract: To improve graft preservation and consequently reduce conservation injuries, the composition of preservation solution is of outmost importance. It was demonstrated that the colloid polyethylene glycol (PEG), used in SCOT solution, has protective effects on cell membranes and immunocamouflage  properties. The aim of this study was to optimize the concentration and chain length of PEG to improve pancreatic islet preservation and outcome. In a model of murine islet allotransplantation, islets were isolated with SCOT containing various concentrations of PEG 20 kDa or 35 kDa. Better  islet yield (IEQ) was obtained with SCO +PEG at 15-30 g/L versus other PEG concentrations and control CMRL-1066 + 1% BSA solution (p < 0.05). Allograft survival was better prolonged (up to 20 days) in the groups SCOT + PEG  20 kDa 10-30 g/L compared to PEG 35 kDa (less than 17.8 days) and to control solutions (less than 17.5 days). In terms of graft function recovery, the use of PEG 20 kDa 15-30 g/L induced no primary nonfunction and delayed graft function contrary to CMRL-1066 and other PEG solutions.  The use of the extracellular-type solution SCOT containing PEG 20 kDa 15 g/L as colloid could be a new way to optimize graft integrity preservation and allograft outcome.
authors.affiliation: INSERM U1082, Poitiers, France.
authors.forename: S
authors.initials: S
authors.lastname: Giraud
authors.forename: D
authors.initials: D
authors.lastname: Bon
authors.forename: Y
authors.initials: Y
authors.lastname: Neuzillet
authors.forename: R
authors.initials: R
authors.lastname: Thuillier
authors.forename: M
authors.initials: M
authors.lastname: Eugene
authors.forename: T
authors.initials: T
authors.lastname: Hauet
authors.forename: B
authors.initials: B
authors.lastname: Barrou
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000601:surgery; D004195:Disease Models, Animal; D007515:Islets of Langerhans; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011092:Polyethylene Glycols
chemical_list: D019188:Organ Preservation Solutions; D011092:Polyethylene Glycols