title: Chemical-biological exploration of the limits of the Ras de- and repalmitoylating machinery.
abstract: A dynamic de-/repalmitoylation cycle determines localization and activity of H- and N-Ras. This combined cellular de- and repalmitoylation machinery has been shown to be substrate tolerant--it accepts variation of amino acid sequence, structure and configuration. Here, semisynthetic Ras-proteins in which the C-terminal amino acids are replaced by peptoid residues are used to reveal the first limitations of substrate recognition by the de- and repalmitoylating machinery.
authors.affiliation: Abteilung Chemische Biologie, Max-Planck-Institut für molekulare Physiologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany.
authors.forename: Kristina
authors.initials: K
authors.lastname: Görmer
authors.forename: Marco
authors.initials: M
authors.lastname: Bürger
authors.forename: John A W
authors.initials: JA
authors.lastname: Kruijtzer
authors.forename: Ingrid
authors.initials: I
authors.lastname: Vetter
authors.forename: Nachiket
authors.initials: N
authors.lastname: Vartak
authors.forename: Lucas
authors.initials: L
authors.lastname: Brunsveld
authors.forename: Philippe I H
authors.initials: PI
authors.lastname: Bastiaens
authors.forename: Rob M J
authors.initials: RM
authors.lastname: Liskamp
authors.forename: Gemma
authors.initials: G
authors.lastname: Triola
authors.forename: Herbert
authors.initials: H
authors.lastname: Waldmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004285:Dogs; D055666:Lipopeptides / Q000737:chemistry*; D054878:Lipoylation; D008245:Lysophospholipase / Q000737:chemistry / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D015398:Signal Transduction; D014162:Transfection; D018631:ras Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D055666:Lipopeptides; D008245:Lysophospholipase; D018631:ras Proteins