title: [Analyze on the component of rosmarinic acid in Prunella vulgaris and its effect on alpha-glycosidase activity].
abstract: OBJECTIVE
  To analyze rosmarinic acid in Prunella vulgaris and its effect on the activity of alpha-glycosidase.
  
  
  METHODS
  Qualitative and quantitative analyses of Rosmarinic acid in Prunella vulgaris were carried out by HPLC. The activity of different micro reaction systems like alpha-amylase, alpha-glucosidase and alpha-maltase, which were added to Rosmarinic acid and Acarbose, was determined by Bernfeld, pNPG and GOD.
  
  
  RESULTS
  The contents of Rosmarinic acid in the aqueous extract and its dry powder, and extractum of Prunella vulgaris were 0.1494, 0.1657 and 0.2739 mg/g respectively, equal to crude drug. The Rosmarinic acid inhibited alpha-glycosidase, and its inhibition from alpha-maltase in small intestine was noncompetitive.
  
  
  CONCLUSIONS
  The aqueous extract of Prunella vulgaris and its extractum's inhibition from alpha-glycosidase is related to Rosmarinic acid.
authors.affiliation: Pre-Clinical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China. wuhuiping625@sohu.com
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Wu
authors.forename: Hong-Lan
authors.initials: HL
authors.lastname: Wang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Xu
authors.forename: Mei-Juan
authors.initials: MJ
authors.lastname: Chen
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Dai
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis* / Q000494:pharmacology*; D053630:Depsides / Q000032:analysis* / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007421:Intestine, Small / Q000201:enzymology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D031347:Prunella / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D053630:Depsides; D004791:Enzyme Inhibitors; D006026:Glycoside Hydrolases; D010936:Plant Extracts; D014867:Water; D000516:alpha-Amylases; D000097123:Rosmarinic Acid