title: Establishment and evaluation of an iELISA using the recombinant membrane protein LHD-Sj23 for the serodiagnosis of Schistosoma japonicum infection in cattle in China.
abstract: Schistosomiasis is an important zoonosis and some livestock especially cattle play a crucial role in disease transmission in endemic areas. In order to establish an effective diagnostic method for detecting Schistosoma japonicum infection in cattle, the gene encoding large hydrophilic domain of Sj23 (LHD-Sj23) was cloned and expressed in Escherichia coli as a fusion protein with GST tag. The purified rLHD-Sj23-GST was used as an antigen in an indirect enzyme-linked immunosorbent assay (iELISA). The sera samples collected from cattle experimentally infected with S. japonicum from week 0 to week 54 post-infection were examined by rLHD-Sj23-GST iELISA. Furthermore, 484 clinical sera samples collected from cattle in schistosomiasis epidemic and free areas in China were tested by this method with a positive rate of 18.4% and 3.44%, respectively. The findings from this study indicated the established iELISA is a useful method for diagnosing S. japonicum infection in cattle and could be used in serological surveys to map out the prevalence of this disease.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: You
authors.initials: Y
authors.lastname: Li
authors.forename: Lixia
authors.initials: L
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Fang
authors.forename: Hao
authors.initials: H
authors.lastname: Nie
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Junlong
authors.initials: J
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Hu
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology* / Q000302:isolation & purification; D012554:Schistosomiasis japonica / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C072633:Sj23 antigen, Schistosoma japonicum