title: New conjugated polymers for photoinduced unwinding of DNA supercoiling and gene regulation.
abstract: Three cationic polythiophene derivatives (P1, P2, P3) were synthesized and characterized. Under white light irradiation (400-800 nm), they sensitize oxygen molecule in the surrounding to generate reactive oxygen species (ROS) that can efficiently unwind the supercoiled DNA in vitro. Further study shows that this relaxation of the DNA supercoiling results in the decrease of gene (pCX-EGFP plasmid) expression level. The ability of these conjugated polymers for regulating gene expression will add a new dimension to the function of conjugated polymers.
authors.affiliation: Beijing National Laboratory for Molecular Science, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences , Beijing, 100190, PR China.
authors.forename: Gaomai
authors.initials: G
authors.lastname: Yang
authors.forename: Huanxiang
authors.initials: H
authors.lastname: Yuan
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhu
authors.forename: Libing
authors.initials: L
authors.lastname: Liu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
authors.forename: Fengting
authors.initials: F
authors.lastname: Lv
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
mesh_terms: D002412:Cations / Q000737:chemistry; D004278:DNA, Superhelical / Q000378:metabolism*; D005786:Gene Expression Regulation; D011108:Polymers / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D002412:Cations; D004278:DNA, Superhelical; D011108:Polymers; D017382:Reactive Oxygen Species; D013876:Thiophenes; C066730:polythiophene