title: High-throughput screening for Streptomyces antibiotic biosynthesis activators.
abstract: A genomic cosmid library of Streptomyces clavuligerus was constructed and transferred efficiently by conjugation to Streptomyces lividans, and 12 distinct groups of overlapping cosmid clones that activated the silent actinorhodin biosynthesis gene cluster were identified. This generally applicable high-throughput screening procedure greatly facilitates the identification of antibiotic biosynthesis activators.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Yemin
authors.initials: Y
authors.lastname: Wang
authors.forename: Hang
authors.initials: H
authors.lastname: Guo
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.forename: Meifeng
authors.initials: M
authors.lastname: Tao
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D003227:Conjugation, Genetic; D003360:Cosmids; D015964:Gene Expression Regulation, Bacterial; D015698:Genomic Library; D057166:High-Throughput Screening Assays; D048170:Streptomyces lividans / Q000235:genetics* / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; C013390:actinorhodin; D000095622:Benzoisochromanequinones