title: Nicotine does not influence NF-κB activity in neonatal mice reoxygenated with room-air or 100% oxygen.
abstract: BACKGROUND
  Nuclear factor kappa B (NF-κB) is considered an important mediator of inflammation, but is also important for developing organs and is constitutive active in neurons in the newborn brain.
  
  
  OBJECTIVE
  We wanted to test the effects of preconditioning with nicotine on neonatal mice subjected to hypoxia and reoxygenated with either room-air or 100% oxygen. We hypothesized that nicotine would be neuroprotective and possibly reduce detrimental effects of 100% oxygen on body organs.
  
  
  METHODS
  In this randomized study, we used neonatal transgenic NF-κB reporter mice that were preconditioned with nicotine or vector only prior to hypoxia, and then subjected to resuscitation with room-air or 100% oxygen.
  
  
  RESULTS
  We did not find any differences in NF-κB activity in whole body or in the brain of animals resuscitated with room-air or 100% oxygen. Resuscitation with 100% oxygen attenuated NF-kB activity when compared with resuscitation with room-air. However, when bioluminescence was properly corrected for body weight, the difference between room-air and 100% reoxygenation was no longer evident.
  
  
  CONCLUSIONS
  Preconditioning with nicotine does not have any effect on NF-κB activity in body organs or in the brain of neonatal mice after hypoxia and resuscitation with either room-air or 100% oxygen. 100% oxygen did not alter NF-κB activity when compared to room-air resuscitation alone.
authors.affiliation: Department of Pediatric Research, Oslo University Hospital, Rikshospitalet, Oslo, Norway.
authors.forename: Jehan Mohamed
authors.initials: JM
authors.lastname: Alsharnoubi
authors.forename: Hans Henrik
authors.initials: HH
authors.lastname: Odland
authors.forename: Ola Didrik
authors.initials: OD
authors.lastname: Saugstad
mesh_terms: D000388:Air; D000818:Animals; D015415:Biomarkers; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D005731:Ganglionic Stimulants / Q000008:administration & dosage / Q000494:pharmacology*; D000860:Hypoxia / Q000378:metabolism* / Q000628:therapy; D016014:Linear Models; D051379:Mice; D008822:Mice, Transgenic; D016328:NF-kappa B / Q000378:metabolism*; D009538:Nicotine / Q000008:administration & dosage / Q000494:pharmacology*; D010102:Oxygen Inhalation Therapy; D011897:Random Allocation; D012151:Resuscitation / Q000379:methods*
chemical_list: D015415:Biomarkers; D005731:Ganglionic Stimulants; D016328:NF-kappa B; D009538:Nicotine