title: Polarization-interferometry-based wavelength-interrogation surface plasmon resonance imager for analysis of microarrays.
abstract: Polarization interferometry (PI) techniques, which are able to improve surface plasmon resonance (SPR) sensing performance and reduce restrictions on allowable parameters of SPR-supporting metal films, have been experimentally realized only in SPR sensors using monochromatic light as a source. Wavelength-interrogation SPR sensors modulated by PI techniques have not been reported due to the wavelength-sensitive characterization of PI phase compensators. In this work we develop a specially designed rhombic prism for phase compensating which is totally insensitive to wavelength. For the first time we successfully apply PI technique to a wavelength-interrogation SPR imager. This imager is able to offer two-dimensional imaging of the whole array plane. As a result of PI modulation, resolutions of 1.3×10(-6) refractive index unit (RIU) under the normal condition and 3.9×10(-7) RIU under a more time-consuming condition are acquired. The application of this imager was demonstrated by reading microarrays for identification of bacteria, and SPR results were confirmed by means of fluorescence imaging.
authors.affiliation: Tsinghua University, Graduate School at Shenzhen, Laboratory of Optical Imaging and Sensing, Shenzhen, 518055, China.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Liu
authors.forename: Le
authors.initials: L
authors.lastname: Liu
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wang
authors.forename: Heng
authors.initials: H
authors.lastname: Shi
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Chong
authors.forename: Suihua
authors.initials: S
authors.lastname: Ma
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Ji
authors.forename: Jihua
authors.initials: J
authors.lastname: Guo
authors.forename: Hui
authors.initials: H
authors.lastname: Ma
authors.forename: Yonghong
authors.initials: Y
authors.lastname: He
mesh_terms: D000465:Algorithms; D015373:Bacterial Typing Techniques; D015374:Biosensing Techniques; D007368:Interferometry / Q000379:methods*; D057230:Limit of Detection; D046228:Microarray Analysis / Q000379:methods*; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*