title: Changing the epidemiology of carbapenem-resistant Pseudomonas aeruginosa in a Brazilian teaching hospital: the replacement of São Paulo metallo-β-lactamase-producing isolates.
abstract: In Brazil, carbapenem-resistant Pseudomonas aeruginosa isolates are closely related to the São Paulo metallo-β-lactamase (SPM) Brazilian clone. In this study, imipenem-resistant isolates were divided in two sets, 2002/2003 and 2008/2009, analysed by pulsed field gel electrophoresis and tested for the Ambler class B metallo-β-lactamase (MBL) genes blaSPM-1, blaIMP and blaVIM. The results show a prevalence of one clone related to the SPM Brazilian clone in 2002/2003. In 2008/2009, P. aeruginosa isolates were mostly MBL negative, genetically diverse and unrelated to those that had been detected earlier. These findings suggest that the resistance to carbapenems by these recent P. aeruginosa isolates was not due to the spread of MBL-positive SPM-related clones, as often observed in Brazilian hospitals.
authors.affiliation: Laboratório de Resistência Microbiana, Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, PE, Brasil.
authors.forename: Felipe Lira de Sá
authors.initials: FL
authors.lastname: Cavalcanti
authors.forename: Anna Carolina Soares
authors.initials: AC
authors.lastname: Almeida
authors.forename: Marinalda Anselmo
authors.initials: MA
authors.lastname: Vilela
authors.forename: Marcia Maria Camargo de
authors.initials: MM
authors.lastname: Morais
authors.forename: Marcos Antonio de
authors.initials: MA
authors.lastname: Morais Junior
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001938:Brazil; D052978:Disk Diffusion Antimicrobial Tests; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006784:Hospitals, Teaching; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D010641:Phenotype; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D018440:beta-Lactam Resistance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem