title: Proliferation and multilineage potential of CXCR4-positive human dental pulp cells in vitro.
abstract: BACKGROUND
  Although the results of our previous studies showed that the stromal cell-derived factor (SDF)-1α-CXC chemokine receptor 4 (CXCR4) axis may play a role in the recruitment of CXCR4-positive dental pulp cells (CXCR4(+) DPCs) toward the damaged sites, the specific function of CXCR4(+) DPCs in the injured dental pulp was still unknown. The purpose of this study was to verify whether CXCR4(+) DPCs possessed stem cells properties so that we can understand their role in area of injury.
  
  
  METHODS
  CXCR4(+) DPCs were isolated from normal DPCs with magnetic-activated cell sorting. The characteristics of the cells from the 3 groups of cells (ie, CXCR4(+) DPCs, CXCR4(-) DPCs, or nonsorted DPCs) were analyzed in colony formation, proliferation, and multilineage differentiation including odontogenic and adipogenic lineages.
  
  
  RESULTS
  The results showed that CXCR4(+) DPCs were the most dominant population in colony formation, proliferation, alkaline phosphatase activity, calcium content, and adipogenic differentiation among the 3 groups.
  
  
  CONCLUSIONS
  CXCR4(+) DPCs may contain more stem cells than nonsorted DPCs.
authors.affiliation: Department of General Dentistry, 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
authors.forename: Long
authors.initials: L
authors.lastname: Jiang
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Peng
authors.forename: Li-Fen
authors.initials: LF
authors.lastname: Li
authors.forename: Ya
authors.initials: Y
authors.lastname: Yang
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Zhu
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D000469:Alkaline Phosphatase / Q000032:analysis; D000880:Anthraquinones; D001391:Azo Compounds; D002113:Calcification, Physiologic / Q000502:physiology; D002118:Calcium / Q000032:analysis; D002452:Cell Count; D018929:Cell Culture Techniques; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000502:physiology; D002633:Chemotaxis / Q000502:physiology; D024042:Collagen Type I / Q000032:analysis; D004396:Coloring Agents; D003470:Culture Media; D003782:Dental Pulp / Q000166:cytology* / Q000293:injuries; D016326:Extracellular Matrix Proteins / Q000032:analysis; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology; D009805:Odontogenesis / Q000502:physiology; D015675:Osteocalcin / Q000032:analysis; D047495:PPAR gamma / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis; D019718:Receptors, CXCR4 / Q000502:physiology*; D012795:Sialoglycoproteins / Q000032:analysis; D013234:Stem Cells / Q000502:physiology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D000880:Anthraquinones; D001391:Azo Compounds; D002118:Calcium; D054377:Chemokine CXCL12; D024042:Collagen Type I; D004396:Coloring Agents; D003470:Culture Media; D016326:Extracellular Matrix Proteins; D015675:Osteocalcin; D047495:PPAR gamma; D010750:Phosphoproteins; D019718:Receptors, CXCR4; D012795:Sialoglycoproteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D000099311:Dentin Sialophosphoprotein; C527276:CXCR4 protein, human; C010078:alizarin; C022616:thiazolyl blue; C011049:oil red O