title: Purification and characterization of the acetone carboxylase of Cupriavidus metallidurans strain CH34.
abstract: Acetone carboxylase (Acx) is a key enzyme involved in the biodegradation of acetone by bacteria. Except for the Helicobacteraceae family, genome analyses revealed that bacteria that possess an Acx, such as Cupriavidus metallidurans strain CH34, are associated with soil. The Acx of CH34 forms the heterohexameric complex α(2)β(2)γ(2) and can carboxylate only acetone and 2-butanone in an ATP-dependent reaction to acetoacetate and 3-keto-2-methylbutyrate, respectively.
authors.affiliation: Department of Proteomics and Microbiology, Interdisciplinary Center of Mass Spectrometry (CISMa), University of Mons, Mons, Belgium.
authors.forename: Caroline
authors.initials: C
authors.lastname: Rosier
authors.forename: Natalie
authors.initials: N
authors.lastname: Leys
authors.forename: Céline
authors.initials: C
authors.lastname: Henoumont
authors.forename: Max
authors.initials: M
authors.lastname: Mergeay
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
mesh_terms: D000096:Acetone / Q000378:metabolism; D002074:Butanones / Q000378:metabolism; D002262:Carboxy-Lyases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D049921:Cupriavidus / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D055503:Protein Multimerization; D021122:Protein Subunits; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D002074:Butanones; D021122:Protein Subunits; D000096:Acetone; C005222:methylethyl ketone; D002262:Carboxy-Lyases; C100963:acetone carboxylase