title: Changes in gene expression in a porcine preadipocyte cell line during differentiation.
abstract: Adipocyte differentiation plays an important role in the formation of fat tissues in pigs and affects meat quality and productivity. Clarification of the nature of the pig genes that participate in adipocyte differentiation will provide a clue to the regulation of fat content and thickness in pig carcases by dietary control; it will also help to find target genes for exploring potentially useful polymorphisms for molecular breeding aimed at fat traits. We constructed a DNA oligomer microarray based on pig transcripts, and we used the array to investigate time-dependent changes in gene expression in the PSPA porcine preadipocyte cell line during differentiation into adipocytes. We selected genes with markedly altered expression (at least fivefold difference in comparison with expression in undifferentiated cells) and classified them into five groups according to gene expression pattern. In the early stage after stimulation of adipocyte differentiation, we observed up-regulation of many genes encoding proteins involved in regulating cell proliferation and transcription. Among the probes corresponding to transcripts that showed marked changes in expression, 27 were located within previously reported QTL regions for traits related to adipose tissues. These results will be valuable resources for finding the genes responsible for fat-related traits that have been identified in previous studies using various pig resource families.
authors.affiliation: Animal Research Division, Institute of Society for Techno-innovation of Agriculture, Forestry and Fisheries, 446-1 Kamiyokoba, Tsukuba, Ibaraki, 305-0854, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: I
authors.initials: I
authors.lastname: Nakajima
authors.forename: T
authors.initials: T
authors.lastname: Eguchi-Ogawa
authors.forename: Y
authors.initials: Y
authors.lastname: Nagamura
authors.forename: N
authors.initials: N
authors.lastname: Hamasima
authors.forename: H
authors.initials: H
authors.lastname: Uenishi
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D018076:DNA, Complementary / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D040641:Quantitative Trait Loci; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary