title: Laser microdissection and mass spectrometry-based proteomics aids the diagnosis and typing of renal amyloidosis.
abstract: Accurate diagnosis and typing of renal amyloidosis is critical for prognosis, genetic counseling, and treatment. Laser microdissection and mass spectrometry are emerging techniques for the analysis and diagnosis of many renal diseases. Here we present the results of laser microdissection and mass spectrometry performed on 127 cases of renal amyloidosis during 2008-2010. We found the following proteins in the amyloid deposits: immunoglobulin light and heavy chains, secondary reactive serum amyloid A protein, leukocyte cell-derived chemotaxin-2, fibrinogen-α chain, transthyretin, apolipoprotein A-I and A-IV, gelsolin, and β-2 microglobulin. Thus, laser microdissection of affected areas within the kidney followed by mass spectrometry provides a direct test of the composition of the deposit and forms a useful ancillary technique for the accurate diagnosis and typing of renal amyloidosis in a single procedure.
authors.affiliation: Division of Anatomic Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA. sethi.sanjeev@mayo.edu
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Sethi
authors.forename: Julie A
authors.initials: JA
authors.lastname: Vrana
authors.forename: Jason D
authors.initials: JD
authors.lastname: Theis
authors.forename: Nelson
authors.initials: N
authors.lastname: Leung
authors.forename: Anjali
authors.initials: A
authors.lastname: Sethi
authors.forename: Samih H
authors.initials: SH
authors.lastname: Nasr
authors.forename: Fernando C
authors.initials: FC
authors.lastname: Fervenza
authors.forename: Lynn D
authors.initials: LD
authors.lastname: Cornell
authors.forename: Mary E
authors.initials: ME
authors.lastname: Fidler
authors.forename: Ahmet
authors.initials: A
authors.lastname: Dogan
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000686:Amyloidosis / Q000145:classification / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D016022:Case-Control Studies; D006801:Humans; D007674:Kidney Diseases / Q000145:classification / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D007678:Kidney Glomerulus / Q000737:chemistry* / Q000276:immunology; D061346:Laser Capture Microdissection; D008910:Minnesota; D008969:Molecular Sequence Data; D011237:Predictive Value of Tests; D011379:Prognosis; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D011506:Proteins