title: E-cadherin mediates adhesion and endocytosis of Aspergillus fumigatus blastospores in human epithelial cells.
abstract: BACKGROUND
  Aspergillus fumigatus (A. fumigatus) is a ubiquitous saprophytic fungus responsible for the majority of invasive mold infections in patients undergoing chemotherapy, organ transplantation or with persistent neutropenia. This study aimed to determine the role of E-cadherin for adhesion and endocytosis of A. fumigatus blastospores in the human epithelial cell line A549.
  
  
  METHODS
  A. fumigatus blastospores were incubated with the total protein of A549 to investigate the binding of E-cadherin and blastospores followed by an affinity purification procedure. After establishing the adhesion model, the adhesion and endocytosis of A. fumigatus blastospores by A549 cells were evaluated by down-regulating E-cadherin of A549 cells using blocking antibody or small interfering RNA (siRNA).
  
  
  RESULTS
  E-cadherin was adhered to the surface of A. fumigatus blastospore. Adhesion and endocytosis of the blastospores were reduced by blocking or down-regulating E-cadherin in A549 cells.
  
  
  CONCLUSIONS
  E-cadherin is a receptor for adhesion and endocytosis of A. fumigatus blastospores in epithelial cells. This may open a new approach to treat this fungal infection.
authors.affiliation: Department of Respiratory Medicine, Second Military Medical University/Nanjing General Hospital of Nanjing Military Command, People's Liberation Army, Nanjing, Jiangsu, China.
authors.forename: Xiao-yong
authors.initials: XY
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
authors.forename: Peng-peng
authors.initials: PP
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Yu-ying
authors.initials: YY
authors.lastname: Shen
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.forename: Bei-lei
authors.initials: BL
authors.lastname: Zhao
mesh_terms: D001232:Aspergillus fumigatus / Q000166:cytology*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004705:Endocytosis / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology*; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D011485:Protein Binding / Q000502:physiology; D034741:RNA, Small Interfering; D013172:Spores, Fungal / Q000166:cytology*
chemical_list: D015820:Cadherins; D005656:Fungal Proteins; D034741:RNA, Small Interfering