title: Effects of the composite nanovesicles on the physical properties and cellular adhesion of chitosan films.
abstract: Chitosan films were prepared by the casting of a chitosan gel in absence and presence of composite nanovesicles. The microscopy images showed the occurrence of agglomerates on the surface and internal pores when the nanovesicles were added to the films, differently from the smooth surface of the pure chitosan films. Despite the hydrophobic character that composite nanovesicles gave to the chitosan films, as showed by the reduction of the water permeation at prolonged times, there was a reduction on the contact angle values for these samples related to the roughness of the surface. The peak of water desorption observed on calorimetric analysis of chitosan was shifted to higher values when the nanovesicles were added to the films. Furthermore, the desappearance of Tg on the films containing nanovesicles denoted their plastifier effect in the chitosan film. The swelling results showed higher water diffusion at the first times for the films containing nanovesicles because of the pores observed by microscopy. However, at prolonged times, there was a reduction on the swelling because of the lipofilic composition of the nanovesicles. Furthermore, the presence of nanovesicles led to a reduction on the water content in the chitosan films. Due to the effect on the physical properties of the chitosan films, the addition of nanovesicles on discrete concentrations contributed to the cell adhesion.
authors.affiliation: Instituto de Química, Universidade Federal do Rio Grande do Sul Av. Bento Gonçalves, 9500, CEP 91501-970 C.P. 15003, Porto Alegre - Brazil.
authors.forename: Maria I Z
authors.initials: MI
authors.lastname: Lionzo
authors.forename: Giulia C
authors.initials: GC
authors.lastname: Lorenzini
authors.forename: Joelson
authors.initials: J
authors.lastname: Tomedi
authors.forename: Patricia
authors.initials: P
authors.lastname: Pranke
authors.forename: Nádya P
authors.initials: NP
authors.lastname: Silveira
mesh_terms: D000107:Acetylation; D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D049329:Nanostructures / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties / Q000187:drug effects; D013696:Temperature; D014867:Water
chemical_list: D001672:Biocompatible Materials; D014867:Water; D048271:Chitosan