title: Standardization and quality controls for the methylated DNA immunoprecipitation technique.
abstract: MeDIP (Methylated DNA Immunoprecipitation) is a relatively recent technique aimed to enrich the methylated fraction of DNA with an antibody directed against 5-methyl-cytosine. MeDIP processed samples are suitable for investigation of the methylation status of specific genomic loci and for performing genome-wide screening when hybridized to DNA methylation microarrays or analyzed by deep sequencing. Here, we describe a standardization protocol and quality controls to assess the specificity, reproducibility and efficiency of the MeDIP procedure. These may have utility when comparing results between samples and experiments within laboratories and between laboratories.
authors.affiliation: Centre for Integrated Systems Biology of Aging and Nutrition; Institute for Aging and Health; Newcastle University; Newcastle upon Tyne, UK. sofia.lisanti1@newcastle.ac.uk
authors.forename: Sofia
authors.initials: S
authors.lastname: Lisanti
authors.forename: Thomas
authors.initials: T
authors.lastname: von Zglinicki
authors.forename: John C
authors.initials: JC
authors.lastname: Mathers
mesh_terms: D018899:CpG Islands; D019175:DNA Methylation; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D047468:Immunoprecipitation / Q000379:methods / Q000592:standards*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards*; D011786:Quality Control