title: Neutrophil gelatinase-associated lipocalin protects renal tubular epithelial cell in ischemic/reperfusion injury rats via apoptosis-regulating proteins.
abstract: We investigated the role of neutrophil gelatinase-associated lipocalin (NGAL) on renal tubular epithelial cell in the renal ischemia/reperfusion injury (IRI) rats. Male Sprague-Dawley rats were randomly assigned to three groups. The control group (n = 5) underwent left nephrectomy. The ischemia/reperfusion (I/R) + normal saline (NS) (n = 5) and I/R + NGAL groups (n = 5) were subjected to 45 min right renal ischemia followed by 48 h of reperfusion after left nephrectomy. The pathological changes of kidney tissues were investigated using hematoxylin-eosin staining; renal tubular epithelial cell apoptosis was detected using terminal dUTP nick-labeling method; expression of apoptosis-regulating protein Fas and Bcl-2 was measured using real-time polymerase chain reaction, Western blot, and immunohistochemical staining. Compared with I/R + NS group, kidney tissues from I/R + NGAL group revealed reduced histological damage and a decreased number of renal tubular epithelial cell apoptosis (9.2 ± 2.53 nuclei or 4.0 ± 0.7 per high-power field vs. 20.3 ± 3.7 nuclei or 8.1 ± 0.3 per high-power field); rats with NGAL showed downregulated fas mRNA (2.34 ± 0.51 vs. 6.84 ± 2.34), fas protein (0.65 ± 0.05 vs. 0.95 ± 0.08), and upregulated bcl-2 protein (0.33 ± 0.05 vs. 0.24 ± 0.03). The results had statistical significance (p < 0.05). We think NGAL could protect against renal IRI and might be related to decreasing tubular epithelial cell apoptosis via adjusting the expression of apoptosis-regulating proteins.
authors.affiliation: Department of Nephrology, Shanghai First People's Hospital Affiliated to Jiaotong University, Shanghai, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Gong
authors.forename: Hua
authors.initials: H
authors.lastname: Yu
authors.forename: Yifeng
authors.initials: Y
authors.lastname: ZhuGe
authors.forename: Qing
authors.initials: Q
authors.lastname: Yu
mesh_terms: D000209:Acute-Phase Proteins / Q000494:pharmacology*; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D007124:Immunoenzyme Techniques; D020287:In Situ Nick-End Labeling; D007684:Kidney Tubules / Q000166:cytology* / Q000473:pathology; D000071068:Lipocalin-2; D054834:Lipocalins / Q000494:pharmacology*; D008297:Male; D018832:Molecular Chaperones; D009392:Nephrectomy; D009687:Nuclear Proteins / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000494:pharmacology*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000503:physiopathology* / Q000517:prevention & control*; D051025:bcl-Associated Death Protein / Q000378:metabolism
chemical_list: D000209:Acute-Phase Proteins; D048868:Adaptor Proteins, Signal Transducing; C494821:Bad protein, rat; C528558:Daxx protein, rat; C097278:Lcn2 protein, rat; D000071068:Lipocalin-2; D054834:Lipocalins; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D051025:bcl-Associated Death Protein