title: Comparative in vitro study of the intestinal absorption of titanium and iron in rats.
abstract: Rat Everted Gut Sac (EGS) model was employed to study the intestinal uptake of titanium and iron. Incubation of freshly prepared rat EGS in Earle's medium pH = 7.4 containing titanium showed that the absorption of titanium as well as iron was a dose dependent process. Ascorbic acid enhanced the absorption of both metal ions, while NaF (1 mM) as an inhibitor of glycolytic energy supply, decreased their absorption. The Na+-K+ ATPase inhibitor, ouabain (1 mM) reduced intestinal absorption of Titanium. This suggests that titanium uptake is an active transport process as is iron uptake. Iron absorption was reduced approximate by 17% when titanium was presented to incubation medium EGS whereas, the absorption of titanium was decreased by 35% when iron was added to the reaction mixture.
authors.affiliation: Department of Biochemistry, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Aarabi
authors.forename: Ali Asghar
authors.initials: AA
authors.lastname: Moshtaghie
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mirhashemi
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D001693:Biological Transport, Active / Q000187:drug effects; D019343:Citric Acid / Q000493:pharmacokinetics; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D058085:Iron Compounds / Q000493:pharmacokinetics*; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D014025:Titanium / Q000493:pharmacokinetics*
chemical_list: D058085:Iron Compounds; C419387:titanium citrate; D019343:Citric Acid; D010042:Ouabain; D012969:Sodium Fluoride; D014025:Titanium; D000254:Sodium-Potassium-Exchanging ATPase; D001205:Ascorbic Acid