title: The interaction of leukocytes and adhesion molecules in mesenteric microvessel endothelial cells after internal capsule hemorrhage.
abstract: OBJECTIVE
  To explore the correlation between hemorheological variations and the expression of cell adhesion molecules in mesenteric microvessel endothelial cells after internal capsule hemorrhage.
  
  
  METHODS
  We established an internal capsule hemorrhage model. Then leukocyte-endothelium interaction was observed and hemorheological variations in mesenteric microvessels were evaluated in the following aspects: blood flow volume, diameter of microvessels, blood flow rate, and shear rate. We also measured the expression of vascular cell adhesion molecule-l and intercellular adhesion molecule-1 (ICAM-1) in mesenteric microvessel endothelial cells with immunohistochemistry stain.
  
  
  RESULTS
  Leukocyte-endothelium interaction intensified after internal capsule hemorrhage. Besides, blood flow volume and velocity decreased, diameter narrowed, and shear rate reduced. Immunohistochemical staining of vascular cell adhesion molecule-l and ICAM-1in mesenteric microvessel endothelial cells was stronger.
  
  
  CONCLUSIONS
  VCAM-1 and ICAM-1 expression in mesenteric microvessels increased as a result of decreased wall shear stress in stress state following internal capsule hemorrhage, and then further shear stress change from interaction of enhanced production of CAMs and leukocytes created a vicious cycle of leukocytes margination, adhesion, and transmigration that could ultimately result in stress gastrointestinal ulcer.
authors.affiliation: Department of Physiology, Tianjin Medical University, Tianjin, China.
authors.forename: Xue-Long
authors.initials: XL
authors.lastname: Jin
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Li
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002450:Cell Communication; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D006470:Hemorrhage / Q000378:metabolism* / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007962:Leukocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013152:Splanchnic Circulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*
chemical_list: D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1