title: Application of a fluorescent derivatization reagent 9-chloromethyl anthracene on determination of carboxylic acids by HPLC.
abstract: A simple and sensitive high-performance liquid chromatography (HPLC) method is proposed for the analysis of some carboxylic acids in food samples and the environment. The use of 9-chloromethyl anthracene as a fluorescence-labeling reagent has been investigated. The derivatization reagent reacts with unitary carboxylic acids and tetrabutylammonium bromide as a catalyst within 50 min in acetonitrile to give esters, which can be separated by HPLC employing fluorescence detection at λ(ex) = 365 and λ(em) = 410 nm. The optimum conditions for derivatization, fluorescence detection and chromatographic separation are established. The method shows good sensitivity, with a detection limit from 0.18 to 2.53 pmol, and good linearity between 1-250 nmol/mL of each analyte. The practical applicability of the method was demonstrated by analyzing samples that were spiked with the acid standards, environment and food samples.
authors.affiliation: Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China. raretea@126.com
authors.forename: Zhenming
authors.initials: Z
authors.lastname: Xie
authors.forename: Lin
authors.initials: L
authors.lastname: Yu
authors.forename: Huijuan
authors.initials: H
authors.lastname: Yu
authors.forename: Qinying
authors.initials: Q
authors.lastname: Deng
mesh_terms: D000873:Anthracenes / Q000737:chemistry*; D001628:Beverages / Q000032:analysis*; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis
chemical_list: D000873:Anthracenes; D002264:Carboxylic Acids; D012989:Soil Pollutants; C099162:9-chloromethylanthracene