title: Combining quantitative trait loci and heterogeneous microarray data analyses reveals putative candidate pathways affecting mastitis in cattle.
abstract: Mastitis is a frequent disease and considerable problem for the global dairy industry. Identification of solutions leading to the development of new control strategies is therefore of high importance. In this study, we have integrated genomic data from genome-wide association mapping in cattle with transcriptomic data from microarray studies of several mastitis pathogens and host species in vitro and in vivo. To identify significant candidate pathways directly and indirectly involved in the immune response to mastitis, ingenuity pathway analysis (ipa) and database for annotation, visualization and integrated discovery bioinformatic (david) were applied. Several candidate pathways were found. Of great interest are IL-17 and IL-8 signalling pathways, responsible for the recruitment and migration of inflammatory cells into tissue during inflammation and infection. These results may emphasize further functional studies for identification of factors contributing to resistance to mastitis pathogens in cattle.
authors.affiliation: Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, NO-0033, Oslo, Norway. anna.sabat@nvh.no
authors.forename: A M
authors.initials: AM
authors.lastname: Lewandowska-Sabat
authors.forename: J
authors.initials: J
authors.lastname: Günther
authors.forename: H M
authors.initials: HM
authors.lastname: Seyfert
authors.forename: I
authors.initials: I
authors.lastname: Olsaker
mesh_terms: D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D005260:Female; D055106:Genome-Wide Association Study; D020381:Interleukin-17 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D008264:Macrophages / Q000276:immunology; D008321:Mammary Glands, Animal / Q000276:immunology* / Q000473:pathology*; D008414:Mastitis, Bovine / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D040641:Quantitative Trait Loci; D015398:Signal Transduction
chemical_list: D020381:Interleukin-17; D016209:Interleukin-8