title: Hyperbranched polylysine: a versatile, biodegradable transfection agent for the production of recombinant proteins by transient gene expression and the transfection of primary cells.
abstract: The feasibility of a new transfection agent, HBPL, for the production of recombinant IgG antibody via TGE as well as for the transfection of primary cells is studied. Under the conditions investigated, transfection of CHO-DG44 cells using HBPL results in IgG yields that are comparable to those obtained with PEI. In experiments with CHO-K1 cells and MEFs the use of HPBL allows to achieve transfection efficiencies comparable to or better than those obtained with PEI or Fugene®. HBPL-mediated transfection does not require complex pre-formation, works well in serum-containing media and is biodegradable, which may prevent cumulative cytotoxicity and facilitates downstream processing.
authors.affiliation: Institut des Matériaux et Institut des Sciences et Ingénierie Chimiques, École Polytechnique Fédérale de Lausanne-EPFL, Bâtiment MXD, Station 12, CH-1015 Lausanne, Switzerland.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kadlecova
authors.forename: Yashas
authors.initials: Y
authors.lastname: Rajendra
authors.forename: Mattia
authors.initials: M
authors.lastname: Matasci
authors.forename: David
authors.initials: D
authors.lastname: Hacker
authors.forename: Lucia
authors.initials: L
authors.lastname: Baldi
authors.forename: Florian Maria
authors.initials: FM
authors.lastname: Wurm
authors.forename: Harm-Anton
authors.initials: HA
authors.lastname: Klok
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D015870:Gene Expression; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics; D011107:Polylysine / Q000737:chemistry*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection / Q000379:methods*
chemical_list: D007074:Immunoglobulin G; D011994:Recombinant Proteins; D011107:Polylysine