title: [Changes of glycogen synthase kinase-3β and its phosphorylation in spinal cord neurons in rats with incisional pain-remifentanil-induced hyperalgesia].
abstract: OBJECTIVE
  To investigate the change in glycogen synthase kinase-3β (GSK-3β) in spinal cord neurons in rats with incisional pain (IP)-remifentanil-induced hyperalgesia.
  
  
  METHODS
  32 SD male rats (240 - 260 g) were randomly divided into 4 groups (n = 8 each): group R, group I, group R + I and group C. IP was established as Brennan's description. Paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were measured 24h before anesthesia and 2, 6, 24, 48 h after anesthesia. The rats were sacrificed after the last threshold measurement. The expressions of GSK-3β mRNA in rats' spinal cord neurons were determined by real-time PCR. The expressions of GSK-3β and pGSK-3β in rats' spinal cord neurons were determined by Western blot.
  
  
  RESULTS
  Remifentanil-induced hyperalgesia developed in group R, I and R + I. The expression of GSK-3β mRNA and the expression of GSK-3β in rats' spinal cord neurons were highest in group R + I. In addition, the ratio of pGSK-3β/GSK-3β was smallest in group R + I.
  
  
  CONCLUSIONS
  These data indicate that the increased GSK-3β activity in rats spinal cord neurons is involved in remifentanil-induced hyperalgesia.
authors.affiliation: Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Jing-Yao
authors.initials: JY
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.forename: Yong-Hao
authors.initials: YH
authors.lastname: Yu
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Wang
mesh_terms: D000818:Animals; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D006930:Hyperalgesia / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D010880:Piperidines / Q000009:adverse effects* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077208:Remifentanil; D013116:Spinal Cord / Q000166:cytology; D013127:Spinal Nerves / Q000378:metabolism*
chemical_list: D010880:Piperidines; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D038362:Glycogen Synthase Kinase 3; D000077208:Remifentanil