title: A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay.
abstract: Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 °C for 66 days.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Byoung Chan
authors.initials: BC
authors.lastname: Kim
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Oh
authors.forename: Jungbae
authors.initials: J
authors.lastname: Kim
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D057075:Enzyme Assays; D057230:Limit of Detection; D013917:Thrombin / Q000032:analysis* / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D013917:Thrombin