title: [Optimization of the ultrasound assisted extraction of saikosaponins from Bupleurum falcatum using response surface methodology].
abstract: OBJECTIVE
  To investigate the optimization of extraction conditions of saikosaponins a, c and d from Bupleurum falcatum.
  
  
  METHODS
  Experimental factors and levels were firstly selected by one-factor test. According to the central composite experimental design principle, the response surface methodology with 3 factors and 3 levels was adopted. Used HPLC to determine the contents of saikosaponins a, c and d. Used column symmetry C18 (150 mm x 4.6 mm, 5 microm); Mobile phase was acetonitrile-water gradient elution, flow rate was 1.0 mL/min, the detection wavelength was 200 nm, detecting temperature was 25 degrees C.
  
  
  RESULTS
  The optimum conditions of aikosaponins extraction were as follows:extraction time was 93 min, power was 150 W, solvent to solid ratio was 60. The predicted total saikosaponin extraction yield was 13.66 mg/g,while the actual extraction yield was 13.64 mg/g, with relative error of 0.15%.
  
  
  CONCLUSIONS
  The optimum extraction process is reasonable, reliable and high yield of extracting effective content.
authors.affiliation: State Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Taian 271018, China. sunyinshi2002@163.com
authors.forename: Yin-Shi
authors.initials: YS
authors.lastname: Sun
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Zhu
authors.forename: He-Feng
authors.initials: HF
authors.lastname: Jiang
authors.forename: Sheng-Hai
authors.initials: SH
authors.lastname: Zhang
mesh_terms: D029972:Bupleurum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics
chemical_list: D012503:Saponins; D012997:Solvents; D000431:Ethanol; D009828:Oleanolic Acid; C025759:saikosaponin D