title: A pretreatment method for HPLC analysis of cypermethrin in microbial degradation systems.
abstract: In this paper, a pretreatment method for high-performance liquid chromatography (HPLC) determination of cypermethrin (CY) in microbial degradation systems was systemically studied, primarily to solve the problem of inaccurate determination of CY concentration caused by its uneven distribution in the systems. A suitable pretreatment method was established, including sampling, extraction and dehydration of CY. Partial sampling could be taken for bacterial and yeast systems in which CY was uniformly dispersed by an emulsifying agent, while total sampling was only suitable for mold systems with or without an emulsifying agent. CY could be fully extracted from the samples in which microbial cells were disrupted by ultrasonic treatment with acetonitrile under ultrasonic condition. The extract could be effectively dehydrated and purified by passing it through an anhydrous Na(2)SO(4) column followed by an elution with acetonitrile. The determination of CY in the pretreated sample by HPLC showed a high precision [relative standard deviation (RSD) = 1.14%, n = 5] and a good stability over a period of five days (RSD = 1.57%, n = 5). The recoveries of CY in microbial degradation systems at three different spiked levels ranged from 95.68 to 108.09% (RSD = 0.50-5.87%, n = 5).
authors.affiliation: College of Light Industry and Food, Sichuan University, 24 South Section One, Yihuan Road, Chengdu 610065, PR China.
authors.forename: Shuliang
authors.initials: S
authors.lastname: Liu
authors.forename: Kai
authors.initials: K
authors.lastname: Yao
authors.forename: Dongying
authors.initials: D
authors.lastname: Jia
authors.forename: Nan
authors.initials: N
authors.lastname: Zhao
authors.forename: Wen
authors.initials: W
authors.lastname: Lai
authors.forename: Huaiyu
authors.initials: H
authors.lastname: Yuan
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005658:Fungi / Q000378:metabolism*; D010575:Pesticides / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D011722:Pyrethrins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D010575:Pesticides; D011722:Pyrethrins; C017160:cypermethrin