title: A beginner's guide to eukaryotic genome annotation.
abstract: The falling cost of genome sequencing is having a marked impact on the research community with respect to which genomes are sequenced and how and where they are annotated. Genome annotation projects have generally become small-scale affairs that are often carried out by an individual laboratory. Although annotating a eukaryotic genome assembly is now within the reach of non-experts, it remains a challenging task. Here we provide an overview of the genome annotation process and the available tools and describe some best-practice approaches.
authors.affiliation: Department of Human Genetics, Eccles Institute of Human Genetics, School of Medicine, University of Utah, Salt Lake City, Utah 84112-5330, USA. myandell@genetics.utah.edu
authors.forename: Mark
authors.initials: M
authors.lastname: Yandell
authors.forename: Daniel
authors.initials: D
authors.lastname: Ence
mesh_terms: D001483:Base Sequence; D030541:Databases, Genetic; D056890:Eukaryota / Q000235:genetics*; D005091:Exons; D016678:Genome; D023281:Genomics; D006801:Humans; D007438:Introns; D058977:Molecular Sequence Annotation / Q000379:methods* / Q000592:standards*; D008969:Molecular Sequence Data; D011786:Quality Control; D012313:RNA / Q000235:genetics; D016415:Sequence Alignment; D012984:Software
chemical_list: D012313:RNA