title: Association of common polymorphisms in the LRP6 gene with sporadic coronary artery disease in a Chinese population.
abstract: BACKGROUND
  Genetic factors contribute to the development of coronary artery disease (CAD). Recently, a missense mutation in the low density lipoprotein receptor related protein 6 (LRP6) gene, encoding low density lipoprotein receptor related protein 6, has been implicated in an autosomal dominant form of early-onset CAD. The aim of this study was to determine whether the common variants in LRP6 are associated with sporadic CAD in Chinese.
  
  
  METHODS
  A total of 766 CAD patients and 806 healthy controls were included in this study. The presence of angiographic CAD was determined by coronary angiographic analysis. Six signal nucleotide polymorphisms (SNPs) were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.
  
  
  RESULTS
  A significant association was detected between rs11054731 in LRP6 intron 2 and CAD in our cohort (P = 0.001). The CC genotype and C allele frequency in the case group were 52% and 72%. Using a dominant model of inheritance, the C allele of rs11054731 was shown to be an independent risk factor for CAD with an OR of 1.45 (95%CI: 1.19 - 1.77, P = 0.0002). With the stratification according to the number of affected coronary arteries, an association was observed between rs11054731 and CAD (P = 0.0002). No significant association was observed between any other SNPs and the risk of CAD.
  
  
  CONCLUSIONS
  The C allele of the rs11054731 within the LRP6 gene was associated with increased risk and extent of CAD in Chinese.
authors.affiliation: Key laboratory for Experimental Teratology of the Ministry of Education and Department of Medical Genetics, Shandong University School of Medicine, Jinan, Shandong 250012, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Qi-Ji
authors.initials: QJ
authors.lastname: Liu
authors.forename: Min-Zhi
authors.initials: MZ
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Cheng
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.forename: Yao-Qin
authors.initials: YQ
authors.lastname: Gong
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D060488:Low Density Lipoprotein Receptor-Related Protein-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D060488:Low Density Lipoprotein Receptor-Related Protein-6