title: Can the individual calibration be modified when laser fluorescence method is used for caries detection?
abstract: BACKGROUND
  Individual calibration (IC) for caries detection methods based on fluorescence is time-consuming, especially for paediatric dentists, if the calibration has to be performed tooth-by-tooth. However, it is not clear how this calibration actually interfere in laser fluorescence (LF) readings.
  
  
  OBJECTIVE
  This in vivo study was to verify the influence of different modes of IC on laser fluorescence (LF) readings.
  
  
  METHODS
  Ninety six occlusal and 95 buccal surfaces of 1st permanent molars were examined using LF device after IC performed on control (no IC), the examined teeth, a permanent incisor, a 1st primary molar or a 2nd primary molar. All modes of IC were performed in the same child. Wilcoxon test and Bland-Altman analysis were used to compare the readings. Intraclass correlation coefficients (ICC) were calculated.
  
  
  RESULTS
  Laser fluorescence readings without prior calibration were higher than readings performed after any mode of IC and resulted in different values of ICC. After other IC modes, the LF readings were statistically similar.
  
  
  CONCLUSIONS
  The absence of IC influences LF readings and LF reproducibility, but different IC methods can be considered in clinical practice.
authors.affiliation: Departmento de Ortodontia e Odontopediatria, Faculdade de Odontologia da Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Reyes
authors.forename: Gisele E
authors.initials: GE
authors.lastname: Ferreira
authors.forename: Joyce
authors.initials: J
authors.lastname: Santos
authors.forename: Fausto M
authors.initials: FM
authors.lastname: Mendes
authors.forename: Jose C P
authors.initials: JC
authors.lastname: Imparato
authors.forename: Mariana M
authors.initials: MM
authors.lastname: Braga
mesh_terms: D001938:Brazil; D002138:Calibration / Q000592:standards; D002648:Child; D002675:Child, Preschool; D003627:Data Interpretation, Statistical; D003731:Dental Caries / Q000175:diagnosis*; D005453:Fluorescence; D006801:Humans; D007834:Lasers; D015588:Observer Variation; D018709:Statistics, Nonparametric; D013997:Time Factors