title: A novel recombinant Leishmania donovani p45, a partial coding region of methionine aminopeptidase, generates protective immunity by inducing a Th1 stimulatory response against experimental visceral leishmaniasis.
abstract: The development of a vaccine against visceral leishmaniasis (VL) conferring long-lasting immunity remains a challenge. Identification and proteomic characterization of parasite proteins led to the detection of p45, a member of the methionine aminopeptidase family. To our knowledge the present study is the first known report that describes the molecular and immunological characterization of p45. Recombinant Leishmania donovani p45 (rLdp45) induced cellular responses in cured hamsters and generated Th1-type cytokines from peripheral blood mononuclear cells of cured/endemic VL patients. Immunization with rLdp45 exerted considerable prophylactic efficacy (∼85%) supported by an increase in mRNA expression of iNOS, IFN-γ, TNF-α and IL-12 and decrease in TGF-β and IL-4, indicating its potential as a vaccine candidate against VL.
authors.affiliation: Division of Parasitology, Central Drug Research Institute, Lucknow 226001, India.
authors.forename: Reema
authors.initials: R
authors.lastname: Gupta
authors.forename: Pramod K
authors.initials: PK
authors.lastname: Kushawaha
authors.forename: Chandra Dev Pati
authors.initials: CD
authors.lastname: Tripathi
authors.forename: Shyam
authors.initials: S
authors.lastname: Sundar
authors.forename: Anuradha
authors.initials: A
authors.lastname: Dube
mesh_terms: D000293:Adolescent; D000328:Adult; D000626:Aminopeptidases / Q000235:genetics / Q000276:immunology*; D000818:Animals; D002648:Child; D002675:Child, Preschool; D006224:Cricetinae; D016207:Cytokines / Q000378:metabolism; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007893:Leishmania donovani / Q000235:genetics / Q000276:immunology*; D054332:Leishmaniasis Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000517:prevention & control*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008647:Mesocricetus; D063208:Methionyl Aminopeptidases; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D018417:Th1 Cells / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D016207:Cytokines; D016054:DNA, Protozoan; D054332:Leishmaniasis Vaccines; D014614:Vaccines, Synthetic; D000626:Aminopeptidases; D063208:Methionyl Aminopeptidases