title: VEGF and MMP-9: biomarkers for canine lymphoma.
abstract: Vascular endothelial growth factor (VEGF) and metalloproteinase (MMP) 2 and 9 are useful biomarkers in human lymphoma. During cancerogenesis, transforming growth factor beta (TGF-β) stimulates VEGF and MMPs production. VEGF and TGF-β plasma levels were tested by ELISA, MMP-2 and MMP-9 by gelatine zymography in 37 dogs with lymphoma, 13 of which were also monitored during chemotherapy. Ten healthy dogs served as control. Lymphoma dogs showed higher act-MMP-9 (P < 0.01) and VEGF (P < 0.05), and lower TGF-β than controls, and a positive correlation between act-MMP-9 and VEGF (P < 0.001). Act-MMP-9 and VEGF were significantly higher in T-cell lymphomas, and in stage V compared with stages III-IV disease, regardless of immunophenotype. VEGF was higher in high-grade compared with low-grade T-cell lymphomas. No correlation was found between cytokines levels at presentation and outcome. During chemotherapy, act-MMP-9 and VEGF decreased in B-cell lymphomas (P < 0.01), suggesting a possible predictive role in this group of dogs.
authors.affiliation: Department of Public Health, Comparative Pathology and Veterinary Hygiene, Faculty of Veterinary Medicine, University of Padova, Legnaro, PD, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Aresu
authors.forename: A
authors.initials: A
authors.lastname: Aricò
authors.forename: S
authors.initials: S
authors.lastname: Comazzi
authors.forename: M E
authors.initials: ME
authors.lastname: Gelain
authors.forename: F
authors.initials: F
authors.lastname: Riondato
authors.forename: M
authors.initials: M
authors.lastname: Mortarino
authors.forename: E
authors.initials: E
authors.lastname: Morello
authors.forename: D
authors.initials: D
authors.lastname: Stefanello
authors.forename: M
authors.initials: M
authors.lastname: Castagnaro
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D014408:Biomarkers, Tumor; D016207:Cytokines / Q000235:genetics; D004283:Dog Diseases / Q000188:drug therapy / Q000378:metabolism*; D004285:Dogs; D008223:Lymphoma / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D020780:Matrix Metalloproteinase 9 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000235:genetics / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D016207:Cytokines; D042461:Vascular Endothelial Growth Factor A; D020780:Matrix Metalloproteinase 9