title: Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli.
abstract: This study was conducted to determine the effects of the antimicrobial peptide cecropin on performance and intestinal health in piglets. Newly weaned barrows were randomly assigned to one of three treatments (n=8), including a corn-soybean basal diet or similar diets supplemented with antibiotics (100 mg/kg kitasamycin plus 800 mg/kg colistin sulfate) or 400 mg/kg cecropin AD. On day 13, all piglets were orally challenged with 10(9)CFU/mL of Escherichia coli K88. On day 19, all piglets were euthanized and sampled. Before challenge, piglets fed antibiotics had greater weight gain, feed efficiency, nitrogen and energy retention than the control (P<0.05). E. coli challenge decreased weight gain, feed intake and feed efficiency for the control piglets (P<0.05) but not for the antibiotic or cecropin AD treated piglets. The incidence of diarrhea post-challenge in the antibiotic and cecropin AD treatments decreased compared with the control piglets. The total viable counts of cecal E. coli were lower while the Lactobacilli counts were higher in the antibiotic and cecropin AD treatments compared with the control (P<0.05). Cecropin AD treatment decreased total aerobes while increasing total anaerobes in the ileum (P<0.05). A higher villus height to crypt depth ratio in the jejunum and ileum as well as a deeper crypt depth in the jejunum and higher villus height in the ileum were observed in piglets fed antibiotics or cecropin AD compared with control piglets (P<0.05). Piglets fed the control diet had lower levels of secretory IgA in their jejunum and lower serum IgA, IgG, interleukin-1β and interleukin-6 compared with the other treatments (P<0.05). Overall, these data suggest that cecropin AD enhances pig performance through increasing immune status and nitrogen and energy retention as well as reducing intestinal pathogens in weaned piglets.
authors.affiliation: State Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Centre, China Agricultural University, Beijing 100193, China.
authors.forename: Shudan
authors.initials: S
authors.lastname: Wu
authors.forename: Fengrui
authors.initials: F
authors.lastname: Zhang
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Huang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Chunyuan
authors.initials: C
authors.lastname: Xie
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhang
authors.forename: Philip A
authors.initials: PA
authors.lastname: Thacker
authors.forename: Shiyan
authors.initials: S
authors.lastname: Qiao
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage*; D003091:Colistin / Q000494:pharmacology; D003967:Diarrhea / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary; D004435:Eating / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D007071:Immunoglobulin A, Secretory / Q000032:analysis / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D019476:Insect Proteins / Q000008:administration & dosage*; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007422:Intestines / Q000187:drug effects / Q000382:microbiology / Q000502:physiology; D015939:Kitasamycin / Q000494:pharmacology; D007778:Lactobacillus / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000276:immunology; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D019476:Insect Proteins; D053583:Interleukin-1beta; D015850:Interleukin-6; C084230:cecropin AD protein, insect; D015939:Kitasamycin; D003091:Colistin