title: Immune responses of zebrafish (Danio rerio) induced by bath-vaccination with a live attenuated Vibrio anguillarum vaccine candidate.
abstract: A fish vaccine candidate, live attenuated Vibrio anguillarum, which can protect fish from vibriosis, was established in our laboratory. In this study, the protective immunological mechanism of live attenuated V. anguillarum was investigated in zebrafish as a model animal. After bath-vaccinated with the live attenuated strain, zebrafish were challenged with wild pathogenic strain to test the immunoprotection of the live attenuated strain. As the results, specific antibody response of fish against V. anguillarum was found to gradually increase during 28 days post-vaccination, and remarkable protection was showed with a high relative protection survival (RPS) of about 90%. Moreover, the vaccination changed the expressions of several immune-related genes in the spleens and livers of zebrafish. Among them, the expressions of pro-inflammatory factors such as IL-1 and IL-8 were tenderly up-regulated with about 3-4 fold in 1-7 days post-vaccination, while MHC II rose to a peak level of 4-fold in 7th day post-vaccination. These results gave some important messages about the mechanism of specific protection induced by live attenuated V. anguillarum and showed the availability of zebrafish model in the evaluation of the vaccine candidate.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhang
authors.forename: Haizhen
authors.initials: H
authors.lastname: Wu
authors.forename: Jingfan
authors.initials: J
authors.lastname: Xiao
authors.forename: Qiyao
authors.initials: Q
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D005393:Fish Diseases / Q000401:mortality / Q000517:prevention & control*; D020869:Gene Expression Profiling; D007075:Immunoglobulin M / Q000097:blood; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D016019:Survival Analysis; D014613:Vaccines, Attenuated / Q000276:immunology; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000401:mortality / Q000517:prevention & control / Q000662:veterinary*; D015027:Zebrafish / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007075:Immunoglobulin M; D007375:Interleukin-1; D016209:Interleukin-8; D014613:Vaccines, Attenuated