title: Asynchronous magnetic bead rotation microviscometer for rapid, sensitive, and label-free studies of bacterial growth and drug sensitivity.
abstract: The long turnaround time in antimicrobial susceptibility testing (AST) endangers patients and encourages the administration of wide spectrum antibiotics, thus resulting in alarming increases of multidrug resistant pathogens. A method for faster detection of bacterial proliferation presents one avenue toward addressing this global concern. We report on a label-free asynchronous magnetic bead rotation (AMBR) based viscometry method that rapidly detects bacterial growth and determines drug sensitivity by measuring changes in the suspension's viscosity. With this platform, we observed the growth of a uropathogenic Escherichia coli isolate, with an initial concentration of 50 cells per drop, within 20 min; in addition, we determined the gentamicin minimum inhibitory concentration (MIC) of the E. coli isolate within 100 min. We thus demonstrated a label-free, microviscometer platform that can measure bacterial growth and drug susceptibility more rapidly, with lower initial bacterial counts than existing commercial systems, and potentially with any microbial strains.
authors.affiliation: Department of Chemistry, University of Michigan, 930 North University, Ann Arbor, Michigan 48109-1055, USA.
authors.forename: Irene
authors.initials: I
authors.lastname: Sinn
authors.forename: Theodore
authors.initials: T
authors.lastname: Albertson
authors.forename: Paivo
authors.initials: P
authors.lastname: Kinnunen
authors.forename: David N
authors.initials: DN
authors.lastname: Breslauer
authors.forename: Brandon H
authors.initials: BH
authors.lastname: McNaughton
authors.forename: Mark A
authors.initials: MA
authors.lastname: Burns
authors.forename: Raoul
authors.initials: R
authors.lastname: Kopelman
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development*; D005839:Gentamicins / Q000494:pharmacology; D059346:Magnets; D008863:Microspheres; D055616:Microtechnology / Q000295:instrumentation*; D012399:Rotation; D013997:Time Factors; D014783:Viscosity
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins