title: cGMP-dependent protein kinase I is involved in neurite outgrowth via a Rho effector, rhotekin, in Neuro2A neuroblastoma cells.
abstract: Although the cGMP/cGMP-dependent protein kinase (cGK) signaling is involved in the regulation of neurite outgrowth, its mechanism remains to be clarified. In this study, we identified a Rho effector, rhotekin, as a cGK-I-interacting protein. Rhotekin was also a substrate for cGK-Iα. In neurite-extended Neuro2A neuroblastoma cells, cGK-Iα and rhotekin were colocalized in the plasma membrane and extended neurites, while treatment with cGMP resulted in translocation of rhotekin to the cytoplasm. In addition, we found that cGK-Iα and rhotekin synergistically suppressed Rho-induced neurite retraction. Our findings suggest that cGK-Iα interacts with and phosphorylates rhotekin, thereby contributing to neurite outgrowth regulation.
authors.affiliation: Department of Biological Science and Technology, The University of Tokushima Graduate School, Tokushima 770-8506, Japan. yuasa@bio.tokushima-u.ac.jp
authors.forename: Keizo
authors.initials: K
authors.lastname: Yuasa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagame
authors.forename: Makoto
authors.initials: M
authors.lastname: Dohi
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Yanagita
authors.forename: Shin
authors.initials: S
authors.lastname: Yamagami
authors.forename: Masami
authors.initials: M
authors.lastname: Nagahama
authors.forename: Akihiko
authors.initials: A
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins; D045744:Cell Line, Tumor; D062965:Cyclic GMP-Dependent Protein Kinase Type I; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism*; D019204:GTP-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016501:Neurites / Q000502:physiology*; D010766:Phosphorylation; D012694:Serine / Q000235:genetics / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; C498395:Rtkn protein, mouse; D012694:Serine; D062965:Cyclic GMP-Dependent Protein Kinase Type I; D017869:Cyclic GMP-Dependent Protein Kinases; C569720:Prkg1 protein, mouse; D019204:GTP-Binding Proteins