title: A novel anticancer and antifungus phenazine derivative from a marine actinomycete BM-17.
abstract: A marine actinomycete, designated strain BM-17, was isolated from a sediment sample collected in the Arctic Ocean. The strain was identified as Nocardia dassonvillei based on morphological, cultural, physiological, biochemical characteristics, along with the cell wall analysis and 16S rDNA gene sequence analysis. A new secondary metabolite (1), N-(2-hydroxyphenyl)-2-phenazinamine (NHP), and six known antibiotics (2-7) have been isolated from the saline culture broth of the stain by sequentially purification over macroporous resin D101, silica gel, Sephadex LH-20 column chromatography and preparative HPLC after the stain was incubated in soy bean media at 28°C for 7 days. The chemical structures of the compounds were elucidated on the basis of spectroscopic analysis, including two-dimensional (2D) NMR and HR-ESI-MS data. The new compound showed significant antifungal activity against Candida albicans, with a MIC of 64 μg/ml and high cancer cell cytotoxicity against HepG2, A549, HCT-116 and COC1 cells.
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu 210009, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Gao
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Lu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Xing
authors.forename: Yihua
authors.initials: Y
authors.lastname: Ma
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Lu
authors.forename: Weiwei
authors.initials: W
authors.lastname: Bao
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Xi
mesh_terms: D039903:Actinobacteria / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D001110:Arctic Regions; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D008386:Marine Biology / Q000379:methods; D009615:Nocardia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D009792:Oceans and Seas; D010619:Phenazines / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000970:Antineoplastic Agents; D001688:Biological Products; D010619:Phenazines