title: Active/inactive dual-probe system for selective photoaffinity labeling of small molecule-binding proteins.
abstract: Two are better than one: A new approach to selective photoaffinity labeling is described in which a bioactive probe is used in combination with its inactive analog as a scavenger of nonspecific proteins.
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, Japan. sakuraik@cc.tuat.ac.jp
authors.forename: Kaori
authors.initials: K
authors.lastname: Sakurai
authors.forename: Masaki
authors.initials: M
authors.lastname: Tawa
authors.forename: Ayumi
authors.initials: A
authors.lastname: Okada
authors.forename: Rika
authors.initials: R
authors.lastname: Yamada
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
authors.forename: Masahiro
authors.initials: M
authors.lastname: Inahara
authors.forename: Maia
authors.initials: M
authors.lastname: Inoue
mesh_terms: D000818:Animals; D024402:Carbonic Anhydrase II / Q000378:metabolism*; D002417:Cattle; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D019920:Photoaffinity Labels / Q000032:analysis* / Q000378:metabolism; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D054852:Small Molecule Libraries / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008024:Ligands; D019920:Photoaffinity Labels; D054852:Small Molecule Libraries; D012710:Serum Albumin, Bovine; D024402:Carbonic Anhydrase II