title: [Direct incorporation of 18O isotopes into peptides and proteins for quantative analysis by mass spectrometry method].
abstract: The method of direct introduction 18O isotopes in peptides and proteins carboxylic groups through the exchange with H218O in the presence of TFA is shown. The isotope label is steady enough in awide range of pH. Because the labeled compounds retain their physical and chemical characteristics, they can be used as an internal standard in quantitative determination of authentic compounds in the analyzed sites by mass spectrometry methods. The technique may be applicable for quantitative analysis of peptides and proteins in biological environments, for quantitative study of the kinetics of metabolism and enzymatic activity. For polypeptides and proteins the quantitative analysis is combined with trypsinolysis. If necessary, the isotope label may be introduced simultaneously in all peptides and proteins control bioassays, making it suitable for use as a standard for the comparative analysis of experimental bioassays.
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Koz'min
authors.forename: A V
authors.initials: AV
authors.lastname: Manoĭlov
authors.forename: M V
authors.initials: MV
authors.lastname: Serebriakova
authors.forename: O A
authors.initials: OA
authors.lastname: Mirgorodskaia
mesh_terms: D000809:Angiotensins / Q000032:analysis; D000818:Animals; D002417:Cattle; D006801:Humans; D010103:Oxygen Isotopes / Q000032:analysis* / Q000737:chemistry*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D012709:Serum Albumin / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014362:Trypsinogen / Q000032:analysis; D014867:Water / Q000737:chemistry
chemical_list: D000809:Angiotensins; D010103:Oxygen Isotopes; D010455:Peptides; D011506:Proteins; D012709:Serum Albumin; D014867:Water; D014362:Trypsinogen