title: Lipase is essential for the study of in vitro release kinetics from organogels.
abstract: In vitro drug release studies remain indispensable in the development of drug delivery systems, even if correlations between in vitro and in vivo results are often imperfect. In this work, an improved in vitro analysis method for studying in situ-forming lipid-based implants was developed. More specifically, lipase was found to be an essential additive for evidencing differences in drug release kinetics from organogels of different amino acid-based organogelators, organogelator concentrations, drug loadings, and volumes. Lipases are thought to participate in the degradation of and release from amino acid-based organogel implants in vivo. Our experimental conditions allowed for the rapid and reliable screening of in vitro parameters that may be optimized to slow or accelerate drug release, once preliminary in vivo data are available.
authors.affiliation: Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology Zürich (ETHZ), Wolfgang-Pauli Str. 10, 8093 Zürich, Switzerland.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Dufresne
authors.forename: Elaheh
authors.initials: E
authors.lastname: Marouf
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Kränzlin
authors.forename: Marc A
authors.initials: MA
authors.lastname: Gauthier
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Leroux
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002511:Cephalosporins / Q000737:chemistry*; D016503:Drug Delivery Systems; D005782:Gels / Q000737:chemistry*; D007700:Kinetics; D008049:Lipase / Q000235:genetics*; D012450:Safflower Oil / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D005782:Gels; D012450:Safflower Oil; C053503:ceftiofur; D008049:Lipase