title: Identification of a new genotype of bovine leukemia virus.
abstract: To investigate the degree of genetic variability of bovine leukemia virus (BLV) strains circulating in Croatia, 29 isolates from the six largest dairy farms were examined by PCR for a segment of the gp51 env gene, followed by DNA sequencing and phylogenetic analysis. The nucleotide sequences were compared with other previously characterized BLV strains from different geographical areas, comprising all seven known BLV genotypes. The Croatian sequences showed six to eight nucleotide substitutions: six silent substitutions and two amino acid changes. Four of those substitutions were within epitopes. In comparison to the sequences of other BLV genotypes, our isolates showed the closest relationship to genotype 1 isolates PL-3252 (FJ808585) and AL-148 (FJ808573) from Argentina. The degree of variation between our sequences and those of genotype 1 was 0.2- 4.6 %. In phylogenetic trees based on 400-nt and 519-nt sequences, all of the Croatian sequences clustered separately from the other sequences, revealing a new genotype.
authors.affiliation: Veterinary Department Vinkovci, Croatian Veterinary Institute, Vinkovci, Croatia.
authors.forename: Davor
authors.initials: D
authors.lastname: Balić
authors.forename: Ivana
authors.initials: I
authors.lastname: Lojkić
authors.forename: Marin
authors.initials: M
authors.lastname: Periškić
authors.forename: Tomislav
authors.initials: T
authors.lastname: Bedeković
authors.forename: Andreja
authors.initials: A
authors.lastname: Jungić
authors.forename: Nina
authors.initials: N
authors.lastname: Lemo
authors.forename: Besi
authors.initials: B
authors.lastname: Roić
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Cač
authors.forename: Ljubo
authors.initials: L
authors.lastname: Barbić
authors.forename: Josip
authors.initials: J
authors.lastname: Madić
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017523:Croatia / Q000453:epidemiology; D003612:Dairying; D016583:Enzootic Bovine Leukosis / Q000453:epidemiology / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D014644:Genetic Variation; D005838:Genotype; D001909:Leukemia Virus, Bovine / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D012367:RNA, Viral