title: Recent developments in bacterial protein glycan coupling technology and glycoconjugate vaccine design.
abstract: The discovery of the Campylobacter jejuni N-linked glycosylation system combined with its functional expression in Escherichia coli marked the dawn of a new era in glycoengineering. The process, termed protein glycan coupling technology (PGCT), has, in particular, been applied to the development of glycoconjugate vaccines. In this review, we highlight recent technical developments in this area, including the first structural determination of the coupling enzyme PglB, the use of glycotags for optimal glycan attachment and the possible applications of other glycosylation systems and how these may improve and extend PGCT.
authors.affiliation: London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors.forename: Vanessa S
authors.initials: VS
authors.lastname: Terra
authors.affiliation: London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors.forename: Dominic C
authors.initials: DC
authors.lastname: Mills
authors.affiliation: London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors.forename: Laura E
authors.initials: LE
authors.lastname: Yates
authors.affiliation: London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors.forename: Sherif
authors.initials: S
authors.lastname: Abouelhadid
authors.affiliation: London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors.forename: Jon
authors.initials: J
authors.lastname: Cuccui
authors.affiliation: London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors.forename: Brendan W
authors.initials: BW
authors.lastname: Wren
mesh_terms: D001428:Bacterial Vaccines / Q000737:chemistry* / Q000276:immunology*; D001709:Biotechnology / Q000379:methods*; D016123:Campylobacter jejuni / Q000201:enzymology; D004926:Escherichia coli / Q000235:genetics; D006001:Glycoconjugates / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D011499:Protein Processing, Post-Translational; D013678:Technology, Pharmaceutical / Q000379:methods*; D018074:Vaccines, Conjugate / Q000737:chemistry / Q000276:immunology; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology
chemical_list: D001428:Bacterial Vaccines; D006001:Glycoconjugates; D018074:Vaccines, Conjugate; D014614:Vaccines, Synthetic