title: Micellar liquid chromatographic determination of carbaryl and 1-naphthol in water, soil, and vegetables.
abstract: A liquid chromatographic procedure has been developed for the determination of carbaryl, a phenyl-N-methylcarbamate, and its main metabolite 1-naphthol, using a C18 column (250 mm  ×  4.6 mm) with a micellar mobile phase and fluorescence detection at maximum excitation/emission wavelengths of 225/333 nm, respectively. In the optimization step, surfactants sodium dodecyl sulphate (SDS), Brij-35 and N-cetylpyridinium chloride monohydrate, and organic solvents propanol, butanol, and pentanol were considered. The selected mobile phase was 0.15 M SDS-6% (v/v)-pentanol-0.01 M NaH(2)PO(4) buffered at pH 3. Validation studies, according to the ICH Tripartite Guideline, included linearity (r > 0.999), limit of detection (5 and 18 ng mL(-1), for carbaryl and 1-naphthol, resp.), and limit of quantification (15 and 50 ng mL(-1), for carbaryl and 1-naphthol, resp.), with intra- and interday precisions below 1%, and robustness parameters below 3%. The results show that the procedure was adequate for the routine analysis of these two compounds in water, soil, and vegetables samples.
authors.affiliation: Grup de Química Bioanalítica, Q.F.A., E.S.T.C.E., Universitat Jaume I, 12071 Castelló, Spain.
authors.forename: Mei-Liang
authors.initials: ML
authors.lastname: Chin-Chen
authors.forename: Maria
authors.initials: M
authors.lastname: Rambla-Alegre
authors.forename: Abhilasha
authors.initials: A
authors.lastname: Durgbanshi
authors.forename: Devasish
authors.initials: D
authors.lastname: Bose
authors.forename: Sandeep K
authors.initials: SK
authors.lastname: Mourya
authors.forename: Josep
authors.initials: J
authors.lastname: Esteve-Romero
authors.forename: Samuel
authors.initials: S
authors.lastname: Carda-Broch