title: Low mannose-binding lectin (MBL) is associated with paediatric inflammatory bowel diseases and ileal involvement in patients with Crohn disease.
abstract: BACKGROUND
  Mannose-binding lectin (MBL) is a pattern-recognition molecule of the innate immune system and may be involved in the pathogenesis of inflammatory bowel disease (IBD). Our aim was to assess the prevalence of MBL deficiency in a cohort of patients with paediatric-onset IBD and study whether it is associated with the clinical manifestations, serum antibody formation, or genetic factors.
  
  
  METHODS
  This prospective study included 159 paediatric patients (mean age: 14.0 years) with IBD [107 patients with Crohn disease (CD) and 52 patients with ulcerative colitis (UC)]. Furthermore, 95 controls were investigated. Serum samples were determined for MBL by enzyme-linked immunosorbent assay (ELISA) and for serologic markers [autoantibodies against Saccharomyces cerevisiae (ASCA) and perinuclear components of neutrophils (pANCA)] by indirect immunofluorescent assay. NOD2/CARD15 variants were tested by polymerase chain reaction/restriction fragment length polymorphism.
  
  
  RESULTS
  The MBL serum concentration was significantly lower in IBD patients(both with CD and UC) compared to controls (IBD, p=0.007, CD, p=0.04, UC p=0.004). Prevalence of low MBL level (<500 ng/mL) was significantly higher in both CD and UC groups compared to controls (p=0.002 and p=0.006). Furthermore, low MBL level was associated with isolated ileal involvement (p=0.01) and MBL deficiency (<100 ng/mL) with male gender (p=0.004) in patients with CD. We failed to confirm any correlation between MBL deficiency and serum autoantibodies or NOD2/CARD15 variants.
  
  
  CONCLUSIONS
  Our results suggest that low MBL associated with paediatric-onset IBD and ileal CD may be considered an additional marker of the IBD pathogenesis.
authors.affiliation: Department of Paediatrics, Petz Aladár County and Teaching Hospital, Győr, Hungary.
authors.forename: Marta
authors.initials: M
authors.lastname: Kovacs
authors.forename: Maria
authors.initials: M
authors.lastname: Papp
authors.forename: Peter Laszlo
authors.initials: PL
authors.lastname: Lakatos
authors.forename: Silvia
authors.initials: S
authors.lastname: Jacobsen
authors.forename: Eva
authors.initials: E
authors.lastname: Nemes
authors.forename: Marianne
authors.initials: M
authors.lastname: Polgar
authors.forename: Eniko
authors.initials: E
authors.lastname: Solyom
authors.forename: Piroska
authors.initials: P
authors.lastname: Bodi
authors.forename: Agnes
authors.initials: A
authors.lastname: Horvath
authors.forename: Kriszta
authors.initials: K
authors.lastname: Molnar
authors.forename: Doloresz
authors.initials: D
authors.lastname: Szabo
authors.forename: Aron
authors.initials: A
authors.lastname: Cseh
authors.forename: Katalin Eszter
authors.initials: KE
authors.lastname: Muller
authors.forename: Antal
authors.initials: A
authors.lastname: Dezsofi
authors.forename: Andras
authors.initials: A
authors.lastname: Arato
authors.forename: Gabor
authors.initials: G
authors.lastname: Veres
mesh_terms: D000293:Adolescent; D000328:Adult; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003093:Colitis, Ulcerative / Q000097:blood* / Q000175:diagnosis; D016001:Confidence Intervals; D003424:Crohn Disease / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D007082:Ileum / Q000473:pathology; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000172:deficiency*; D053473:Nod2 Signaling Adaptor Protein / Q000097:blood; D016017:Odds Ratio; D011446:Prospective Studies; D012720:Severity of Illness Index; D012737:Sex Factors; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D015415:Biomarkers; D037601:Mannose-Binding Lectin; C429018:NOD2 protein, human; D053473:Nod2 Signaling Adaptor Protein; D002097:C-Reactive Protein