title: A non-VH1-69 heterosubtypic neutralizing human monoclonal antibody protects mice against H1N1 and H5N1 viruses.
abstract: Influenza viruses are among the most important human pathogens and are responsible for annual epidemics and sporadic, potentially devastating pandemics. The humoral immune response plays an important role in the defense against these viruses, providing protection mainly by producing antibodies directed against the hemagglutinin (HA) glycoprotein. However, their high genetic variability allows the virus to evade the host immune response and the potential protection offered by seasonal vaccines. The emergence of resistance to antiviral drugs in recent years further limits the options available for the control of influenza. The development of alternative strategies for influenza prophylaxis and therapy is therefore urgently needed. In this study, we describe a human monoclonal antibody (PN-SIA49) that recognizes a highly conserved epitope located on the stem region of the HA and able to neutralize a broad spectrum of influenza viruses belonging to different subtypes (H1, H2 and H5). Furthermore, we describe its protective activity in mice after lethal challenge with H1N1 and H5N1 viruses suggesting a potential application in the treatment of influenza virus infections.
authors.affiliation: Laboratory of Microbiology and Virology, Vita-Salute San Raffaele University, Milan, Italy.
authors.forename: Donata
authors.initials: D
authors.lastname: De Marco
authors.forename: Nicola
authors.initials: N
authors.lastname: Clementi
authors.forename: Nicasio
authors.initials: N
authors.lastname: Mancini
authors.forename: Laura
authors.initials: L
authors.lastname: Solforosi
authors.forename: Guisella J
authors.initials: GJ
authors.lastname: Moreno
authors.forename: Xiangjie
authors.initials: X
authors.lastname: Sun
authors.forename: Terrence M
authors.initials: TM
authors.lastname: Tumpey
authors.forename: Larisa V
authors.initials: LV
authors.lastname: Gubareva
authors.forename: Vasiliy
authors.initials: V
authors.lastname: Mishin
authors.forename: Massimo
authors.initials: M
authors.lastname: Clementi
authors.forename: Roberto
authors.initials: R
authors.lastname: Burioni
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use*; D000914:Antibodies, Viral / Q000276:immunology; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology*; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation / Q000235:genetics; D009500:Neutralization Tests; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006388:Hemagglutinins; D007252:Influenza Vaccines