title: Potential role of a quetiapine metabolite in quetiapine-induced neutropenia and agranulocytosis.
abstract: Clozapine was the first of the atypical antipsychotics to be developed, but its use has been restricted because of toxicity issues, particularly the risk of potentially life-threatening drug-induced neutropenia and agranulocytosis, which occurs in about 1% of patients. Bioactivation of clozapine by peroxidases forms a reactive nitrenium ion, which covalently adducts to protein and leads to neutrophil toxicity. The current generation of clozapine-inspired atypical antipsychotics has reduced toxicity through improved potency/decreased dose or through structural modification to prevent peroxidase-catalyzed nitrenium ion formation. Through the substitution of sulfur for the bridging nitrogen found in clozapine, quetiapine does not directly form a nitrenium ion when incubated with myeloperoxidase/H(2)O(2). We present evidence that cytochrome P450 2D6 catalyzes the formation of 7-hydroxyquetiapine, which can be oxidized by human myeloperoxidase to form a reactive quinone-imine and a reactive radical, which may account for the continued, although reduced, neutrophil toxicity. In the presence of myeloperoxidase/H(2)O(2) and glutathione, covalent 7-hydroxyquetiapine-glutathione adducts were formed. Bioactivation of quetiapine was verified in vivo in rat where three 7-hydroxyquetiapine-mercaptate adducts and a 7-hydroxyquetiapine-glutathione adduct were detected from bile after oral dosing. The decreased incidence of agranulocytosis with quetiapine over clozapine is postulated to be due to the lower exposure of the toxic precursor, 7-hydroxyquetiapine versus clozapine, respectively.
authors.affiliation: Department of Molecular Therapeutics, The Scripps Research Institute , Scripps Florida, 130 Scripps Way, Jupiter, FL 33458, United States.
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Li
authors.forename: Michael D
authors.initials: MD
authors.lastname: Cameron
mesh_terms: D000380:Agranulocytosis / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D014150:Antipsychotic Agents / Q000009:adverse effects / Q000378:metabolism*; D003024:Clozapine / Q000031:analogs & derivatives; D003486:Cyanides / Q000378:metabolism; D019389:Cytochrome P-450 CYP2D6 / Q000378:metabolism; D003987:Dibenzothiazepines / Q000009:adverse effects / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D008297:Male; D009503:Neutropenia / Q000139:chemically induced / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D000069348:Quetiapine Fumarate; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D014150:Antipsychotic Agents; D003486:Cyanides; D003987:Dibenzothiazepines; D000069348:Quetiapine Fumarate; D006735:Horseradish Peroxidase; D009195:Peroxidase; D019389:Cytochrome P-450 CYP2D6; D005978:Glutathione; D003024:Clozapine