title: Global gene expression profiling of human osteosarcomas reveals metastasis-associated chemokine pattern.
abstract: Global gene expression analysis was performed on a panel of 23 osteosarcoma samples of primary and metastatic origin using the Applied Biosystems Gene Expression Array System. When comparing the primary tumours with the metastases, we found a significantly increased expression of genes involved in immunological processes, for example coding for cytokines and chemokines, in the metastatic samples. In addition, a comparison of the gene expression in primary samples from patients with or without metastases demonstrated that patients who later developed metastases had high expression of the chemokine (C-X-C motif) receptor 4 (CXCR4), similar to the metastatic samples, suggesting that these signal molecules play an important role in promoting metastasis. Increased knowledge of mechanisms and interactions between specified molecular signalling pathways in osteosarcomas could lead to a more rational strategy for development of targeted therapy.
authors.affiliation: Department of Tumour Biology, The Norwegian Radium Hospital, Oslo University Hospital, 0310 Oslo, Norway.
authors.forename: Heidi M
authors.initials: HM
authors.lastname: Namløs
authors.forename: Stine H
authors.initials: SH
authors.lastname: Kresse
authors.forename: Christoph R
authors.initials: CR
authors.lastname: Müller
authors.forename: Jørn
authors.initials: J
authors.lastname: Henriksen
authors.forename: Rita
authors.initials: R
authors.lastname: Holdhus
authors.forename: Gunnar
authors.initials: G
authors.lastname: Sæter
authors.forename: Oyvind S
authors.initials: OS
authors.lastname: Bruland
authors.forename: Bodil
authors.initials: B
authors.lastname: Bjerkehagen
authors.forename: Vidar M
authors.initials: VM
authors.lastname: Steen
authors.forename: Ola
authors.initials: O
authors.lastname: Myklebost