title: Complete mitochondrial genome of the seven-spotted lady beetle, Coccinella septempunctata (Coleoptera: Coccinellidae).
abstract: The complete mitochondrial genome (mitogenome) of the seven-spotted lady beetle, Coccinella septempunctata (Coleoptera: Coccinellidae), which is one of the best known insects capable of predation, is described with an emphasis on the noteworthy composition of the A+T-rich region. The C. septempunctata genome consists of 2 rRNAs, 22 tRNAs, 13 protein-coding genes, and 1 control region, designated as the A+T-rich region in insects. Along with an unusually long A+T-rich region (4469 bp), the 18,965-bp long C. septempunctata mitogenome was the largest in Coleoptera. The A+T-rich region is composed of a 2214-bp long non-repeat region composed of 80.17% A/T nucleotides and a 2256-bp long repeat region composed of 65.71% A/T nucleotides. The repeat region harbors 32 identical 70-bp long tandem repeats plus one 15-bp long incomplete first repeat. These repeat sequences may possibly have been caused by slipped-strand mispairing and unequal crossing-over events during DNA replication.
authors.affiliation: Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Min Jee
authors.initials: MJ
authors.lastname: Kim
authors.forename: Xinlong
authors.initials: X
authors.lastname: Wan
authors.forename: Iksoo
authors.initials: I
authors.lastname: Kim
mesh_terms: D020856:AT Rich Sequence / Q000235:genetics; D000818:Animals; D001482:Base Composition; D001517:Coleoptera / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D020459:Genes, rRNA; D049750:Genome, Insect / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins; D012343:RNA, Transfer