title: Chiral analysis by online coupling of reversed-phase liquid chromatography to gas chromatography and mass spectrometry.
abstract: Enantiomeric composition of selected chiral compounds present in complex mixtures is determined by using the online coupling of reversed-phase liquid chromatography (LC) to gas chromatography (GC) and mass spectrometry. Integration of sample preparation into GC analysis, in a completely automated way, is achieved by means of the effective clean-up resulting from both the LC fractionation step and the eluent elimination provided by the through oven transfer adsorption desorption system used for LC-GC interfacing. The possibilities of the technique are illustrated through some examples concerning the stereodifferentiation in essential oils of major and minor chiral compounds via LC-GC transfer of different volume fractions, ranging from 0.5 to 1.9 ml, which show the significance of the window size for the determination of enantiomeric profiles.
authors.affiliation: Instituto de Ciencia y Tecnología de Alimentos y Nutrición, Consejo Superior de Investigaciones Científicas, c/ Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Barba
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Martínez
authors.forename: Marta M
authors.initials: MM
authors.lastname: Calvo
authors.forename: Guillermo
authors.initials: G
authors.lastname: Santa-María
authors.forename: Marta
authors.initials: M
authors.lastname: Herraiz
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000379:methods*; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000032:analysis / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D000077222:Limonene; D039821:Monoterpenes / Q000032:analysis / Q000737:chemistry; D009822:Oils, Volatile / Q000032:analysis / Q000737:chemistry; D009862:Online Systems; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013729:Terpenes / Q000032:analysis / Q000737:chemistry
chemical_list: D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D009822:Oils, Volatile; D013729:Terpenes; C006923:carvone; D000077222:Limonene