title: Acceleration of new bone formation by an electrically polarized hydroxyapatite microgranule/platelet-rich plasma composite.
abstract: We have developed a technology for the electrical polarization and electrical characterization of hydroxyapatite (HA) microgranules. In order to improve handling of ceramic powders to fulfill complex geometrical demands, platelet-rich plasma (PRP) containing many growth factors was chosen as a carrier of HA microgranules. In this study, the effects of this electrically polarized HA microgranule/PRP composite on new bone formation were examined. To compare osteoconductivity, HA microgranules with or without electrical polarization/PRP composite gel, HA microgranules alone with or without electrical polarization, or PRP gel were implanted into holes in the medial femoral condyles of rabbits. As a control, some of the bone holes were left empty (n=6 in each group). Histological examination was performed 3 and 6 weeks after the surgical operation. It was suggested that PRP alone could not induce new bone formation until 6 weeks after implantation. HA microgranules with or without electrical polarization/PRP composite, especially the former, activated osteogenic cells, resulting in enhanced bone formation. It was confirmed that electrical polarization treatment of HA microgranules can accelerate new bone formation, and that this effect is enhanced by forming a complex within the PRP.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda, Japan.
authors.forename: Seiko
authors.initials: S
authors.lastname: Ohba
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Soichiro
authors.initials: S
authors.lastname: Itoh
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Takagi
authors.forename: Akiko
authors.initials: A
authors.lastname: Nagai
authors.forename: Kimihiro
authors.initials: K
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D017886:Durapatite / Q000494:pharmacology*; D004560:Electricity; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology / Q000601:surgery; D020007:Implants, Experimental; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010012:Osteogenesis / Q000187:drug effects*; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D011817:Rabbits; D014961:X-Ray Diffraction; D055114:X-Ray Microtomography
chemical_list: D017886:Durapatite