title: Stilbenoids from Gnetum macrostachyum attenuate human platelet aggregation and adhesion.
abstract: Platelets play a critical role in pathogenesis of cardiovascular disorders and strokes. The inhibition of platelet function is beneficial for the treatment and prevention of these diseases. The phytochemical investigation of stilbenoids from Gnetum macrostachyum Hook. f. led to the isolation of trans-resveratrol (1), isorhapotigenin (2), gnetol (3), bisisorhapontigenin B (4), gnetin C (5), parvifolol A (6), latifolol (7) and gnetuhainin C (8). The isolated stilbenoids were evaluated for in vitro antiplatelet activities via agonist-induced platelet aggregation and static platelet-collagen adhesion assays using washed human platelets. Compounds 1, 2 and 3 were active in the inhibition of arachidonic acid (AA)-induced platelet aggregation. Compound 2 and its dimer, compound 4, were the most active stilbenoids in thrombin-induced platelet aggregation. Moreover, compounds 4, 5 and 6, tended to be more potent than monomeric and trimeric stilbenoids in a human platelet-collagen adhesion assay under static conditions. This is the first report of the antiplatelet activity of stilbenoids isolated from G. macrostachyum.
authors.affiliation: School of Allied Health Sciences, University of Phayao, Phayao, Thailand.
authors.forename: Chiraphat
authors.initials: C
authors.lastname: Kloypan
authors.forename: Rattima
authors.initials: R
authors.lastname: Jeenapongsa
authors.forename: Piyawit
authors.initials: P
authors.lastname: Sri-in
authors.forename: Surin
authors.initials: S
authors.lastname: Chanta
authors.forename: Dech
authors.initials: D
authors.lastname: Dokpuang
authors.forename: Santi
authors.initials: S
authors.lastname: Tip-pyang
authors.forename: Nattanan
authors.initials: N
authors.lastname: Surapinit
mesh_terms: D016718:Arachidonic Acid / Q000494:pharmacology; D035684:Gnetum / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D013267:Stilbenes; D016718:Arachidonic Acid