title: Vaccination of mice with recombinant bacille Calmette-Guérin harboring Rv1357c protects similarly to native BCG.
abstract: Despite the availability of a Mycobacterium bovis bacille Calmette Guérin (BCG) vaccine, tuberculosis (TB) remains a global public health problem. In this study, we introduced the c-di-GMP phosphodiesterase gene Rv1357c, implicated in regulating mycobacterial replication  within macrophages, into BCG Pasteur, and tested the resulting strain for its capacity to serve as a vaccine against TB in a murine model. Modified BCG was more phagocytosed than its parental strain, but halted bacterial replication, and protected against M. tuberculosis challenge similarly  to unmodified BCG.
authors.affiliation: Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Guadalajara, Jalisco, Mexico. fl oresv@ciatej.net.mx
authors.forename: M A
authors.initials: MA
authors.lastname: Flores-Valdez
authors.forename: M de Jesús
authors.initials: MJ
authors.lastname: Aceves-Sánchez
authors.forename: S A
authors.initials: SA
authors.lastname: Montero-Pérez
authors.forename: A D
authors.initials: AD
authors.lastname: Sánchez-López
authors.forename: J A
authors.initials: JA
authors.lastname: Gutiérrez-Pabello
authors.forename: R
authors.initials: R
authors.lastname: Hernández-Pando
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000276:immunology*; D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D002460:Cell Line; D004195:Disease Models, Animal; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D010587:Phagocytosis; D013997:Time Factors; D014397:Tuberculosis, Pulmonary / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D001500:BCG Vaccine; D014614:Vaccines, Synthetic; D000217:Acyltransferases; C531527:Rv1347c, Mycobacterium tuberculosis