title: Polymorphisms of Pit-1 gene and its association with growth traits in chicken.
abstract: The Pit-1 gene is involved in regulation of muscle growth through controlling the expression of growth hormone, prolactin, and transforming growth factor-β genes in chicken. The objectives of the study were to explore polymorphisms of the Pit-1 gene and to estimate the effect of these polymorphisms on growth traits in PB-1 and control (broiler strain) and IWI (layer strain) chickens. Single-stranded conformation polymorphism followed by sequencing was performed to reveal polymorphisms of the gene. In total, 10 haplotypes were found across the lines. The mRNA expression of Pit-1 varied among haplogroups and had a significant effect on BW and growth rates. The haplogroups showed a significant effect on BW in wk 7 in PB-1 chickens. In control chickens there was a significant effect at d 1 and in wk 2 and 7, and in IWI strains, there was a significant effect at d 1 and wk 6 and 7. The significant association of haplogroups and growth rate was found between 0 and 2 wk in control and between 0 and 2 and 6 and 7 wk in IWI strains. It was concluded that the Pit-1 gene is polymorphic and has a significant effect on growth traits in chickens.
authors.affiliation: Project Directorate on Poultry, Rajendranagar, Hyderabad, India. bhattacharyatk@gmail.com
authors.forename: T K
authors.initials: TK
authors.lastname: Bhattacharya
authors.forename: R N
authors.initials: RN
authors.lastname: Chatterjee
authors.forename: M
authors.initials: M
authors.lastname: Priyanka
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006239:Haplotypes; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050817:Transcription Factor Pit-1 / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D050817:Transcription Factor Pit-1