title: [Combined effects of interferon γ and γ ray irradiation on A549 cells in vitro].
abstract: OBJECTIVE
  To define the role of interferon-γ on radiotherapy of lung cancer and explore a new way to clinical treatment.
  
  
  METHODS
  A549 cells were exposed to γ ray with or without IFN-γ co-treatment. MTT assay was performed to evaluate cell viability. Western blot was used to observe the expression of P53 protein.
  
  
  RESULTS
  The results showed that co-treatment of IFN-γ decreased the cell viability significantly compared with the γ ray irradiation group (71.4% ± 2.1% vs 44.1% ± 3.1%, n = 7, P < 0.01). In addition, the expression of P53 protein also increased significantly after co-treatment (P < 0.01); Furthermore, the cell cycle was changed obviously in co-treatment group compared with γ ray irradiation group, S phase increased (12.9% vs 20.9%, n = 5, P < 0.05) and also blocked the G2/M phase (28.8% vs 38.9%, n = 5, P < 0.05).
  
  
  CONCLUSIONS
  The results suggested that γ ray irradiation combined with IFN-γ can increase the efficiency of radiotherapy on A549 cells and there is much broad prospect in the clinical treatment of lung cancer.
authors.affiliation: Department of Thoracic-cardio Surgery, the First Affiliated Hospital of the General Hospital of PLA, Beijing 100048, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Zhang
authors.forename: Chang-hai
authors.initials: CH
authors.lastname: Yu
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Bao-shi
authors.initials: BS
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002453:Cell Cycle / Q000187:drug effects* / Q000528:radiation effects*; D002455:Cell Division; D045744:Cell Line, Tumor; D005720:Gamma Rays; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008175:Lung Neoplasms
chemical_list: D007371:Interferon-gamma