title: The anti-ageing potential of a new jasmonic acid derivative (LR2412): in vitro evaluation using reconstructed epidermis Episkin™.
abstract: Jasmonic acid is involved in plant wound repair and tissue regeneration, but no study has been reported in human skin. The effect of a jasmonic acid derivative, tetra-hydro-jasmonic acid (LR2412, 1 and 10 μm) was investigated on an in vitro reconstructed skin model, Episkin™. Using real time RTQPCR studies, results showed an increase in hyaluronan synthase 2 (HAS2) and hyaluronase synthase 3 (HAS3) expression. Furthermore, an increase in hyaluronic acid (HA) deposits in basal and suprabasal layers of the epidermis was observed. The percentage of positive Ki67 keratinocytes in the basal layer as well as the epidermis thickness were seen to increase. Immunohistochemistry studies showed that the synthesis of late differentiation proteins filaggrin and transglutaminase 1 was not modified. The human epidermis is known to thin with age while HA content has been reported to decrease. These results illustrate the potential of LR2412 in counteracting signs of skin ageing.
authors.forename: Jean F
authors.initials: JF
authors.lastname: Michelet
authors.forename: Christian
authors.initials: C
authors.lastname: Olive
authors.forename: Elodie
authors.initials: E
authors.lastname: Rieux
authors.forename: Dominique
authors.initials: D
authors.lastname: Fagot
authors.forename: Lucie
authors.initials: L
authors.lastname: Simonetti
authors.forename: Jean B
authors.initials: JB
authors.lastname: Galey
authors.forename: Maria
authors.initials: M
authors.lastname: Dalko-Csiba
authors.forename: Bruno A
authors.initials: BA
authors.lastname: Bernard
authors.forename: Rui
authors.initials: R
authors.lastname: Pereira
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D004817:Epidermis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D000091344:Filaggrin Proteins; D014453:Glucuronosyltransferase / Q000378:metabolism; D006801:Humans; D000076002:Hyaluronan Synthases; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008954:Models, Biological; D054883:Oxylipins / Q000494:pharmacology*; D011503:Transglutaminases / Q000378:metabolism
chemical_list: D003517:Cyclopentanes; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D016209:Interleukin-8; D007381:Intermediate Filament Proteins; D054883:Oxylipins; C011006:jasmonic acid; D011503:Transglutaminases; C105804:transglutaminase 1; D014453:Glucuronosyltransferase; C101754:HAS2 protein, human; C105458:HAS3 protein, human; D000076002:Hyaluronan Synthases