title: Nonspecific effect of Mycograb on amphotericin B MIC.
abstract: Mycograb C28Y is a recombinant human antibody fragment thought to target HSP-90 and potentiate amphotericin B (AMB). Absence of in vivo efficacy led us to reevaluate its in vitro activity. Interactions between AMB and Mycograb were investigated using a checkerboard design. Addition of Mycograb or various unrelated proteins, including human serum, resulted in similar decreases in the MIC of AMB. Potentiation of AMB by Mycograb appears to be a nonspecific protein effect.
authors.affiliation: Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Richie
authors.forename: M A
authors.initials: MA
authors.lastname: Ghannoum
authors.forename: N
authors.initials: N
authors.lastname: Isham
authors.forename: K V
authors.initials: KV
authors.lastname: Thompson
authors.forename: N S
authors.initials: NS
authors.lastname: Ryder
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D061067:Antibodies, Monoclonal, Humanized; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D000077336:Caspofungin; D004347:Drug Interactions; D054714:Echinocandins / Q000494:pharmacology; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D055666:Lipopeptides; D008826:Microbial Sensitivity Tests
chemical_list: D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; D000666:Amphotericin B; D015725:Fluconazole; C512268:efungumab; D000077336:Caspofungin