title: Purine biosynthesis mutants (purA and purB) of serotype 4b Listeria monocytogenes are severely attenuated for systemic infection in intragastrically inoculated A/J Mice.
abstract: In this study, we demonstrate that purA and purB transposon mutants of serotype 4b Listeria monocytogenes were severely impaired in their ability to colonize the gastrointestinal tract and cause systemic infection of the spleen, liver, and gallbladder following intragastric inoculation of A/J mice. The mutant strains were also impaired in their ability to multiply within Caco-2 human intestinal epithelial cells. Neither mutant was affected in resistance to synthetic gastric fluid (pH 4.5). These findings indicate that purine biosynthesis is critical for gastrointestinal virulence of L. monocytogenes serotype 4b in mice.
authors.affiliation: School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, 53706, USA.
authors.forename: Nancy G
authors.initials: NG
authors.lastname: Faith
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Reha
authors.initials: R
authors.lastname: Azizoglu
authors.forename: Sophia
authors.initials: S
authors.lastname: Kathariou
authors.forename: Charles
authors.initials: C
authors.lastname: Czuprynski
mesh_terms: D000265:Adenylosuccinate Synthase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018988:Bacterial Translocation; D018938:Caco-2 Cells; D005260:Female; D005704:Gallbladder / Q000382:microbiology; D005750:Gastric Juice / Q000737:chemistry; D005759:Gastroenteritis / Q000382:microbiology / Q000503:physiopathology*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity* / Q000502:physiology; D008088:Listeriosis / Q000382:microbiology / Q000503:physiopathology*; D008099:Liver / Q000382:microbiology; D051379:Mice; D008805:Mice, Inbred A; D050296:Microbial Viability; D050505:Mutant Proteins / Q000378:metabolism; D011687:Purines / Q000096:biosynthesis*; D013154:Spleen / Q000382:microbiology; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D007527:Isoenzymes; D050505:Mutant Proteins; D011687:Purines; D000265:Adenylosuccinate Synthase