title: High resolution separation by pressure-driven liquid chromatography in meander extended nanochannels.
abstract: The rapidly developing interest in nanofluidics, which is used to examine liquids on an order that ranges from an attoliter to a femtoliter, correlates with the recent interest in decreased sample amounts, such as in the field of single-cell analysis. In this paper, we have succeeded in the normal phase separation of a 10(1) fL sample with a high number of theoretical plates (10(3) plates), and a fast separation (4 s), using a pressure-driven flow in extended nanochannels. A meander separation channel 1 μm wide was introduced and showed no band broadening at the turns, unlike microchannels (10(1)-10(2) μm wide), which is an important aspect in order to lengthen the channel in a space-limited micro/nanofluidic chip. Our device enables the fast separation of an ultra-small volume of sample with a high number of theoretical plates, and will serve as a general platform for the separation of aL to fL volumes of samples.
authors.affiliation: Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Ishibashi
authors.forename: Kazuma
authors.initials: K
authors.lastname: Mawatari
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kitamori
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008962:Models, Theoretical; D036103:Nanotechnology / Q000295:instrumentation*; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D011312:Pressure
chemical_list: D004364:Pharmaceutical Preparations