title: [Localization of AcMNPV NLA genes in Sf9 cells].
abstract: Nuclear actin which plays a key role in many nucleic processes has become a research hotspot. Baculovirus is the only reported pathogen using nuclear actin to replicate and proliferate. However, little is known about the mechanism of monomeric G-actin accumulation within nuclei of baculovirus-infected cells. It has been reported that AcMNPV ie-1, pe38, ac4, he65, ac102, and ac152 could be required for mediating nuclear localization of G-actin from transiently transfected results in TN-368 cells. In this paper, we found that IE1, AC152, PE38, AC102 localized in the whole cell and PE38, AC102 localized in the nuclear mainly, while both AC4 and HE65 localized in cytoplasm and could be mediated into the nucleus by AC102 and IE1 respectively for the first time. And ie-1 or pe38, ac4, he65 could mediate nuclear G-actin to accumulate partly, while these four genes were sufficient for recruiting G-actin accumulation within the nucleus when driven by promoter OpIE2. Determining the functions of each of these AcMNPV NLA gene products will advance our understanding of baculovirus biology and function of nuclear actin.
authors.affiliation: Key Laboratory of Human Functional Genomics of Jiangsu Province, School of Basic Medical Science, Nanjing Medical University, Nanjing 210093, China. wqian@njmu.edu.cn
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Ji-Zheng
authors.initials: JZ
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Wang
authors.forename: Xin-Wen
authors.initials: XW
authors.lastname: Chen
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D021381:Protein Transport; D018411:Spodoptera / Q000378:metabolism* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D014764:Viral Proteins