title: A novel method to fabricate thermoresponsive microstructures with improved cell attachment/detachment properties.
abstract: A novel, simple, and rapid method to fabricate thermoresponsive micropatterned substrate for cell adhesion, growth, and thermally induced detachment was developed. Thermoresponsive polymer, poly(N-isopropylacrylamide) (PNIPAAm), was grafted onto the surface of polystyrene (PS) film with microstructure by plasma-induced graft polymerization technique. The thermoresponsive micropatterned films were characterized by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, hydrogen nuclear magnetic resonance ((1) H NMR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). These results indicated that the grafting ratio of PNIPAAm increased with increasing roughness of PS film. However, the microstructures on the substrate were not affected by grafted PNIPAAm. The optimal grafting conditions, such as plasma treatment time, monomer concentration, graft polymerization time, and graft medium were investigated. The thermoresponsive micropatterned films were investigated with the fibroblast cell (L929) adhesion, proliferation, and thermally induced detachment assay. The microstructure on the thermoresponsive micropatterned substrate facilitated cell adhesion above the lower critical solution temperature (LCST) of PNIPAAm and cell detachment below the LCST. Moreover, it can be used to regulate cell organization and tissue growth.
authors.affiliation: Department of Chemistry, School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: He
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Nie
authors.forename: Bi-Zhou
authors.initials: BZ
authors.lastname: Chen
authors.forename: Xiang-Xu
authors.initials: XX
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
mesh_terms: D000178:Acrylamides / Q000494:pharmacology; D000180:Acrylic Resins; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D008567:Membranes, Artificial; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009584:Nitrogen / Q000032:analysis; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases / Q000737:chemistry; D058105:Polymerization / Q000187:drug effects; D011108:Polymers / Q000494:pharmacology; D011137:Polystyrenes / Q000737:chemistry* / Q000494:pharmacology; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D008567:Membranes, Artificial; D058626:Plasma Gases; D011108:Polymers; D011137:Polystyrenes; C052970:poly-N-isopropylacrylamide; D009584:Nitrogen