title: Deletion of UL21 causes a delay in the early stages of the herpes simplex virus 1 replication cycle.
abstract: The herpes simplex virus 1 (HSV-1) U(L)21 gene encodes a 62-kDa tegument protein with homologs in the alpha-, beta-, and gammaherpesvirus subfamilies. In the present study, we characterized a novel U(L)21-null virus and its genetic repair to determine whether this protein plays a role in early stages of the HSV-1 replication cycle. Single-step growth analyses, protein synthesis time courses, and mRNA quantifications indicated that the absence of U(L)21 results in a delay early in the HSV-1 replication cycle.
authors.affiliation: Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama, USA.
authors.forename: Ekaette F
authors.initials: EF
authors.lastname: Mbong
authors.forename: Lucille
authors.initials: L
authors.lastname: Woodley
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Frost
authors.forename: Joel D
authors.initials: JD
authors.lastname: Baines
authors.forename: Carol
authors.initials: C
authors.lastname: Duffy
mesh_terms: D002460:Cell Line; D015536:Down-Regulation; D017353:Gene Deletion; D006561:Herpes Simplex / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
chemical_list: D014764:Viral Proteins; C086728:UL21 protein, herpes simplex virus 1