title: Adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa onto nanohydroxyapatite as a bone regeneration material.
abstract: In orthopedics due to the enormous number of surgical procedures involving invasive implant biomaterials, infections have a huge impact in terms of morbidity, mortality, and medical costs. In this study the initial adhesion of several strains namely Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa, to nanohydroxyapatite, previously heat-treated at 725 °C and 1000 °C was assessed. Adherent cells were evaluated by scanning electron microscopy and quantified by confocal laser scanning microscopy and as colony forming units after being released by sonication. The wettability and roughness of samples surfaces were assessed by contact angle measurements and atomic force microscopy, respectively. Nanohydroxyapatite heat-treated at 1000 °C appeared to be more resistant to bacterial adhesion, over time, in five of the six tested strains while the clinical strains isolated from orthopedic infections presented superior ability to adhere, as well as better capacity to produce slime. The increase in materials sintering temperature resulted in increased hydrophobicity and roughness; however, other surface features such as the decrease in surface area and on porosity as well as the decrease on zeta potential may be the aspects that contributed to a lower bacterial adhesion on the materials sintered at 1000 °C.
authors.affiliation: INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal. liliana.grenho@ineb.up.pt
authors.forename: L
authors.initials: L
authors.lastname: Grenho
authors.forename: M C
authors.initials: MC
authors.lastname: Manso
authors.forename: F J
authors.initials: FJ
authors.lastname: Monteiro
authors.forename: M P
authors.initials: MP
authors.lastname: Ferraz
mesh_terms: D001422:Bacterial Adhesion; D001672:Biocompatible Materials; D001861:Bone Regeneration; D015169:Colony Count, Microbial; D017886:Durapatite; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D013211:Staphylococcus aureus / Q000502:physiology*; D013212:Staphylococcus epidermidis / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite