title: [Influence of isoliensinine on activity of CYP3A in rats].
abstract: OBJECTIVE
  To investigate the influence of isoliensinine (IL) on CYP3A enzyme activity.
  
  
  METHODS
  A mixture metabolic system of liver microsome enzymes in vitro was developed. A HPLC method to test the metabolic activity of CYP3A was established with testosterone as a probe. The activities of CYP3A enzymes were measured with different IL concentrations and incubation time with testosterone.
  
  
  RESULTS
  In mixture metabolic system of liver microsome enzymes, the best incubation concentration of testosterone was 200 μmol/L, the best incubation time was 210 min, in this condition the IC₅₀ of IL for CYP3A inhibition was >1 000 μmol/L.
  
  
  CONCLUSIONS
  No significant interaction between IL and CYP3A is detected, which indicates that IL might be used with CYP 3A enzyme substrates.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Li
authors.initials: L
authors.lastname: Nan
authors.forename: Gui-da
authors.initials: GD
authors.lastname: Xuan
mesh_terms: D000818:Animals; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism*; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000494:pharmacology
chemical_list: D007546:Isoquinolines; C499779:isoliensinine; D013739:Testosterone; D051544:Cytochrome P-450 CYP3A