title: Iron-rich complexes in human spleen in hereditary spherocytosis.
abstract: OBJECTIVE
  Tissue iron plays an important role in the development of certain diseases. Although it is one of biogenic elements, its excess induces the reactive oxygen species (ROS) formation. The aim of the present work is to examine the protection against free or loosely bound iron from the view of morphology and chemical composition of iron-rich complexes in human spleen tissues with hereditary spherocytosis (HS) by scanning and transmission electron microscope with energy-dispersive microanalysis (EDX).
  
  
  RESULTS
  The examination of human spleen tissues by scanning and transmission electron microscope showed covering of iron-rich particles. EDX revealed many iron-rich complexes of multi-element composition in HS samples with sulphur and phosphorus as the major elements. Detection was negative in the reference samples.
  
  
  CONCLUSIONS
  The covering of iron-rich particles can be explained by elimination and isolation of ferritin/iron complexes from surrounding environment to prevent the ROS formation. Sulphur, phosphorus and their compounds are probably the most significant elements that influence the ROS formation (Fig. 5, Ref. 16). Full Text in PDF www.elis.sk.
authors.affiliation: Histopatologia Inc., Bratislava, Slovakia.
authors.forename: C
authors.initials: C
authors.lastname: Biro
authors.forename: P
authors.initials: P
authors.lastname: Busikova
authors.forename: G
authors.initials: G
authors.lastname: Fujerikova
authors.forename: O
authors.initials: O
authors.lastname: El-Hassoun
authors.forename: A
authors.initials: A
authors.lastname: Kopaniova
authors.forename: M
authors.initials: M
authors.lastname: Caplovicova
authors.forename: P
authors.initials: P
authors.lastname: Galfiova
authors.forename: V
authors.initials: V
authors.lastname: Sisovsky
authors.forename: M
authors.initials: M
authors.lastname: Kopani
authors.forename: J
authors.initials: J
authors.lastname: Jakubovsky
mesh_terms: D004577:Electron Probe Microanalysis; D006801:Humans; D058085:Iron Compounds / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D013103:Spherocytosis, Hereditary / Q000378:metabolism* / Q000473:pathology; D013154:Spleen / Q000378:metabolism* / Q000473:pathology
chemical_list: D058085:Iron Compounds