title: Long-chain cationic derivatives of PTA (1,3,5-triaza-7-phosphaadamantane) as new components of potential non-viral vectors.
abstract: The purpose of this study was to investigate the potential of new positively charged solid lipid nanoparticles (SLN) to convey nucleic acids. The cationic character of SLN was obtained by adding as cationic molecules two different long-chain cationic phosphines (CP), namely hexadecyl-PTA iodide (CP16) and octadecyl-PTA iodide (CP18). The obtained CP-SLN are characterized by a positive charge on the surface and reproducible dimensions around 220 nm. These nanosystems are able to efficiently bind nucleic acid molecules and to protect DNA from the activity of serum nucleases up to 120 min. Lastly, in vitro experiments demonstrated that CP-SLN exhibit a quite pronounced antiproliferative effect on cultured human K562 erythroleukemic cells and a limited effect as transfecting adjuvant. These data, and particularly the ability of CP-SLN to protect DNA from degradation, encourages further studies aimed at proposing these nanosystems as a potential approach to deliver nucleic acid to cells in living organisms.
authors.affiliation: Department of Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy. crt@unife.it
authors.forename: Rita
authors.initials: R
authors.lastname: Cortesi
authors.forename: Paola
authors.initials: P
authors.lastname: Bergamini
authors.forename: Laura
authors.initials: L
authors.lastname: Ravani
authors.forename: Markus
authors.initials: M
authors.lastname: Drechsler
authors.forename: Andrea
authors.initials: A
authors.lastname: Costenaro
authors.forename: Mirko
authors.initials: M
authors.lastname: Pinotti
authors.forename: Matteo
authors.initials: M
authors.lastname: Campioni
authors.forename: Lorenza
authors.initials: L
authors.lastname: Marvelli
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Esposito
mesh_terms: D000218:Adamantane / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D020014:K562 Cells; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties; D014162:Transfection / Q000379:methods*; D014280:Triglycerides / Q000008:administration & dosage / Q000737:chemistry
chemical_list: C501763:1,3,5-triaza-7-phosphaadamantane; D009943:Organophosphorus Compounds; D014280:Triglycerides; D004247:DNA; C022618:tristearin; D000218:Adamantane