title: Glutamate microinjection in the medial septum of rats decreases paradoxical sleep and increases slow wave sleep.
abstract: The role of the medial septum in suppressing paradoxical sleep and promoting slow wave sleep was suggested on the basis of neurotoxic lesion studies. However, these conclusions need to be substantiated with further experiments, including chemical stimulation studies. In this report, the medial septum was stimulated in adult male rats by microinjection of L-glutamate. Sleep-wakefulness was electrophysiologically recorded, through chronically implanted electrodes, for 2 h before the injection and 4 h after the injection. There was a decrease in paradoxical sleep during the first hour and an increase in slow wave sleep during the second hour after the injection. The present findings not only supported the lesion studies but also showed that the major role of the medial septum is to suppress paradoxical sleep.
authors.affiliation: All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Didhiti
authors.initials: D
authors.lastname: Mukherjee
authors.forename: Mahesh K
authors.initials: MK
authors.lastname: Kaushik
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Jaryal
authors.forename: Velayudhan Mohan
authors.initials: VM
authors.lastname: Kumar
authors.forename: Hruda Nanda
authors.initials: HN
authors.lastname: Mallick
mesh_terms: D000818:Animals; D004567:Electrodes, Implanted; D018698:Glutamic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008845:Microinjections; D051381:Rats; D011921:Rats, Inbred WKY; D012688:Septum Pellucidum / Q000187:drug effects*; D012890:Sleep / Q000187:drug effects*; D012895:Sleep, REM / Q000187:drug effects*; D014851:Wakefulness / Q000502:physiology
chemical_list: D018698:Glutamic Acid