title: Label-free ultrasensitive detection of human telomerase activity using porphyrin-functionalized graphene and electrochemiluminescence technique.
abstract: Using porphyrin-functionalized graphene, we construct a PCR-free, low-cost, rapid, and electrochemiluminenscence (ECL) assay for detection of telomerase activity that has been demonstrated in six different cell lines and can be used as initial screening of G-quadruplex DNA binding agents and telomerase inhibitors. This ECL sensor shows highly sensitive for detection of telomerase with the detection limit as low as 10 HeLa cells mL(-1) .
authors.affiliation: Division of Biological Inorganic Chemistry, State Key laboratory of Rare Earth Resources Utilization, Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Jiasi
authors.initials: J
authors.lastname: Wang
authors.forename: Lingyan
authors.initials: L
authors.lastname: Feng
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.forename: Weili
authors.initials: W
authors.lastname: Wei
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D000818:Animals; D002460:Cell Line; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D006108:Graphite / Q000737:chemistry*; D057809:HEK293 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D020014:K562 Cells; D008163:Luminescent Measurements; D051379:Mice; D041681:NIH 3T3 Cells; D010087:Oxides / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry*; D019098:Telomerase / Q000032:analysis*
chemical_list: D010087:Oxides; D011166:Porphyrins; D006108:Graphite; D019098:Telomerase