title: Metabonomic profiles delineate the effect of traditional Chinese medicine sini decoction on myocardial infarction in rats.
abstract: BACKGROUND
  In spite of great advances in target-oriented Western medicine for treating myocardial infarction (MI), it is still a leading cause of death in a worldwide epidemic. In contrast to Western medicine, Traditional Chinese medicine (TCM) uses a holistic and synergistic approach to restore the balance of Yin-Yang of body energy so the body's normal function can be restored. Sini decoction (SND) is a well-known formula of TCM which has been used to treat MI for many years. However, its holistic activity evaluation and mechanistic understanding are still lacking due to its complex components.
  
  
  RESULTS
  A urinary metabonomic method based on nuclear magnetic resonance and ultra high-performance liquid chromatography coupled to mass spectrometry was developed to characterize MI-related metabolic profiles and delineate the effect of SND on MI. With Elastic Net for classification and selection of biomarkers, nineteen potential biomarkers in rat urine were screened out, primarily related to myocardial energy metabolism, including the glycolysis, citrate cycle, amino acid metabolism, purine metabolism and pyrimidine metabolism. With the altered metabolism pathways as possible drug targets, we systematically analyze the therapeutic effect of SND, which demonstrated that SND administration could provide satisfactory effect on MI through partially regulating the perturbed myocardial energy metabolism.
  
  
  CONCLUSIONS
  Our results showed that metabonomic approach offers a useful tool to identify MI-related biomarkers and provides a new methodological cue for systematically dissecting the underlying efficacies and mechanisms of TCM in treating MI.
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai, China.
authors.forename: Guangguo
authors.initials: G
authors.lastname: Tan
authors.forename: Wenting
authors.initials: W
authors.lastname: Liao
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.forename: Genjing
authors.initials: G
authors.lastname: Yang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhu
authors.forename: Wuhong
authors.initials: W
authors.lastname: Li
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Chai
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Lou
mesh_terms: D000284:Administration, Oral; D000818:Animals; D015415:Biomarkers / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000627:therapeutic use*; D004734:Energy Metabolism / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000503:physiopathology / Q000652:urine; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal; C478756:sini tang