title: Active inclusion body formation using Paenibacillus polymyxa PoxB as a fusion partner in Escherichia coli.
abstract: Overexpression of Paenibacillus polymyxa PoxB in Escherichia coli induced the formation of inclusion bodies. An enzyme assay showed that the inclusion bodies exhibited PoxB activity, indicating that they were biologically active. Fusion of GFP and Bacillus subtilis AmyE to the C-terminus of the PoxB also induced the formation of biologically active aggregates when they were overexpressed in E. coli. Therefore, P. polymyxa PoxB can be used as a fusion partner to promote the formation of active inclusion bodies in E. coli.
authors.affiliation: Systems and Synthetic Biology Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Park
authors.forename: Soo-Keun
authors.initials: SK
authors.lastname: Choi
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D002479:Inclusion Bodies / Q000378:metabolism*; D056507:Paenibacillus / Q000201:enzymology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins