title: Development of reagents to study the turkey's immune response: cloning and characterisation of two turkey cytokines, interleukin (IL)-10 and IL-13.
abstract: The cDNAs of two turkey cytokines, interleukin (IL)-10 and IL-13, were cloned using oligonucleotide primers designed from their chicken orthologues. The coding regions of the chicken and turkey genes are highly conserved, with IL-10 and IL-13 exhibiting 94.1% and 90% nucleotide and 92% and 79.9% amino acid identity respectively. Both showed consistent mRNA expression in turkey lymphoid and gut tissues. Expression in non-lymphoid tissues was more variable but generally highest in the skin and trachea. Recombinant turkey IL-10 was expressed and bioactivity demonstrated by inhibition of IFN-γ synthesis from activated splenocytes. Chicken and turkey IL-10 cross-reacted in functional assays.
authors.affiliation: Institute for Animal Health, Compton, Berkshire RG20 7NN, UK.
authors.forename: Fiona
authors.initials: F
authors.lastname: Powell
authors.forename: Lisa
authors.initials: L
authors.lastname: Rothwell
authors.forename: Michael
authors.initials: M
authors.lastname: Clarkson
authors.forename: Pete
authors.initials: P
authors.lastname: Kaiser
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D003429:Cross Reactions; D007202:Indicators and Reagents; D016753:Interleukin-10 / Q000737:chemistry / Q000235:genetics*; D018793:Interleukin-13 / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D014422:Turkeys / Q000276:immunology*
chemical_list: D030161:Avian Proteins; D007202:Indicators and Reagents; D018793:Interleukin-13; D011994:Recombinant Proteins; D016753:Interleukin-10