title: Determination of 4-methylpiperaine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride in rats' plasma by online-SPE-HPLC-DAD: application to a pharmacokinetic study.
abstract: A specific, simple, and fast online-solid-phase extraction-high performance liquid chromatography-diode array detector (SPE-HPLC-DAD) method was developed and validated to quantify 4-methylpiperaine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride (TM208) in small volume samples of rats' plasma for the first time. In this method, the 50-μL plasma sample was taken to perform protein precipitation with 75 μL methanol, and then 50 μL supernatant containing the target analytes was injected and concentrated automatically in a C18 solid-phase extraction (SPE) cartridge. After that the sample was separated on a C18 RP analytical column and analyzed by DAD. The run cycle time is 6.0 min for each sample, and the calibration curve over the range of 0.03 to 25.00 μg/mL has a good linear relationship (r > 0.9998). The recoveries of the quality control samples were all greater than 90%. The limit of detection and the lowest limit of quantification were 0.01 and 0.03 μg/mL, respectively. Finally, this method was successfully applied to a pharmacokinetic study of TM208 in rats.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, Beijing, China.
authors.forename: Jianmei
authors.initials: J
authors.lastname: Zhang
authors.forename: Wensi
authors.initials: W
authors.lastname: Lin
authors.forename: Xiaona
authors.initials: X
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Yu
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Ling
authors.forename: Ge
authors.initials: G
authors.lastname: Fu
authors.forename: Runtao
authors.initials: R
authors.lastname: Li
authors.forename: Jingrong
authors.initials: J
authors.lastname: Cui
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D008297:Male; D010879:Piperazines / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: C522133:4-methylpiperazine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride; D000970:Antineoplastic Agents; D010879:Piperazines