title: Expression, purification, crystallization and preliminary X-ray diffraction analysis of nurse shark β2-microglobulin.
abstract: β(2)-Microglobulin (β(2)m) is an essential subunit of the major histocompatibility complex (MHC) class I molecule that helps to stabilize the structure of peptide-MHC I (pMHC I). It is also one of the typical immunoglobulin superfamily (IgSF) molecules in the adaptive immune system (AIS). Sharks belong to the cartilaginous fish, which are the oldest jawed vertebrate ancestors with an AIS to exist in the world. Thus, the study of cartilaginous fish β(2)m would help in understanding the evolution of IgSF molecules. In order to demonstrate this, β(2)m from a cartilaginous fish, nurse shark (Ginglymostoma cirratum), was expressed, refolded, purified and crystallized. Diffraction data were collected to a resolution of 2.3 Å. The crystal belonged to space group P3(2)21, with unit-cell parameters a = b = 88.230, c = 67.146 Å. The crystal structure contained two molecules in the asymmetric unit. The results will provide structural information for study of the evolution of β(2)m and IgSF in the AIS.
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Lu
authors.forename: Shugang
authors.initials: S
authors.lastname: Yao
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Zhang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Chun
authors.initials: C
authors.lastname: Xia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D012754:Sharks / Q000235:genetics; D001613:beta 2-Microglobulin / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001613:beta 2-Microglobulin