title: Characterization of magnetic nanoparticles modified with thiol functionalized PAMAM dendron for DNA recovery.
abstract: Magnetic nanoparticles (MNPs) modified with the thiol functionalized polyamidoamine (PAMAM) dendron were synthesized to estimate their DNA recovery capabilities. Aminosilane-modified MNPs and MNPs surrounded by a phospholipid (distearoylphosphatidylethanolamine (DSPE)) bilayer were used as core particles. Cystamine-core PAMAM dendrimers were reduced by dithiothreitol to dendron thiols and chemically conjugated to the core particles. Characterization of the synthesis revealed an increase of the surface amine charge from generation 1 (G1) to G6, starting with an aminosilane initiator. Particle size distribution analysis indicated that G6 PAMAM-modified MNPs exhibited monodispersity in an aqueous solution. G6 PAMAM-MNPs and G6 PAMAM-PE-MNPs synthesized by the proposed method have equivalent DNA recovery abilities to PAMAM-MNPs prepared by the conventional divergent synthesis method. In optimized conditions, 96% of λDNA was recovered using G6 PAMAM-PE-MNPs. Therefore, the method for preparing PAMAM-MNPs and PAMAM-PE-MNPs proposed in this study will be a novel approach for producing DNA carriers for efficient DNA purification by magnetic separation.
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Koganei, Tokyo 184-8588, Japan. tsuyo@cc.tuat.ac.jp
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Keiyu
authors.initials: K
authors.lastname: Shibata
authors.forename: Masahito
authors.initials: M
authors.lastname: Hosokawa
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hatakeyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Arakaki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Gomyo
authors.forename: Takeyuki
authors.initials: T
authors.lastname: Mogi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Taguchi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Wake
authors.forename: Takeo
authors.initials: T
authors.lastname: Tanaami
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsunaga
mesh_terms: D004279:DNA, Viral / Q000737:chemistry*; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D004279:DNA, Viral; D050091:Dendrimers; C104700:PAMAM Starburst; D013438:Sulfhydryl Compounds