title: Endophytic fungi from pigeon pea [Cajanus cajan (L.) Millsp.] produce antioxidant Cajaninstilbene acid.
abstract: In this study, novel endophytic fungi producing cajaninstilbene acid (CSA) from pigeon pea [ Cajanus cajan  (L.) Millsp.] were investigated and screened. CSA has prominent pharmacological activities. A total of 110 endophytic fungi isolates were grouped into 8 genera on the basis of morphological characteristics, and CSA-producing fungi were screened by liquid chromatography-tandem mass spectrometry (LC-MS/MS). According to ITS-rDNA sequences analysis, the CSA-producing fungi were identified as  Fusarium solani  (ERP-07),  Fusarium oxysporum  (ERP-10), and  Fusarium proliferatum  (ERP-13), respectively. The amount of CSA produced by the ERP-13 reached 504.8 ± 20.1 μg/L or 100.5 ± 9.4 μg/g dry weight of mycelium. In a DPPH radical-scavenging assay, when the concentration of fungal CSA was 500 μg/mL, inhibition percentage could reach 80%, which was almost the same as that of standard CSA. This study first reported the natural antioxidant CSA from endophytic fungi F. solani and F. proliferatum isolated from pigeon pea.
authors.affiliation: Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
authors.forename: JinTong
authors.initials: J
authors.lastname: Zhao
authors.forename: YuJie
authors.initials: Y
authors.lastname: Fu
authors.forename: Meng
authors.initials: M
authors.lastname: Luo
authors.forename: YuanGang
authors.initials: Y
authors.lastname: Zu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: ChunJian
authors.initials: C
authors.lastname: Zhao
authors.forename: ChengBo
authors.initials: C
authors.lastname: Gu
mesh_terms: D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D036561:Cajanus / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis / Q000737:chemistry; D005670:Fusarium / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D012459:Salicylates / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D013267:Stilbenes / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology
chemical_list: C549543:3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid; D000975:Antioxidants; D004271:DNA, Fungal; D012459:Salicylates; D013267:Stilbenes