title: The role of CCR7 in allergic airway inflammation induced by house dust mite exposure.
abstract: House dust mite (HDM), the most common allergen, activate both the IgE-associated and innate immune responses. To clarify the process of sensitization, we investigated the role of the CCL21, CCL19, and CCR7 axis in a mouse model of HDM-induced allergic asthma. HDM inhalation without systemic immunization resulted in a HDM-specific IgE response. CCR7-knockout (CCR7KO) mice exhibited greater airway inflammation and IgE responses compared to wild-type mice. We examined FoxP3 expression in these mice to clarify the contribution of regulatory cells to the responses. FoxP3 expression was higher in the lungs but not in the lymph nodes of CCR7KO mice compared to wild-type mice. In CCR7KO mice, FoxP3-positive cells were found in lung, but we observed higher release of IL-13, IL-5, TGF-β, IL-17, and HMGB1 in bronchoalveolar lavage fluid. We demonstrate here that immuno-regulation through CCR7 expression in T cells plays a role in HDM-specific sensitization in the airway.
authors.affiliation: Department of Pharmacotherapy, Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Kawakami
authors.forename: Osamu
authors.initials: O
authors.lastname: Narumoto
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Horiguchi
authors.forename: Satomi
authors.initials: S
authors.lastname: Horiguchi
authors.forename: Naohide
authors.initials: N
authors.lastname: Yamashita
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: Martin
authors.initials: M
authors.lastname: Lipp
authors.forename: Takahide
authors.initials: T
authors.lastname: Nagase
authors.forename: Naomi
authors.initials: N
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D001249:Asthma / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D051858:Forkhead Transcription Factors / Q000276:immunology; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D039981:Pyroglyphidae / Q000276:immunology*; D054400:Receptors, CCR7 / Q000172:deficiency / Q000276:immunology*
chemical_list: C515371:Ccr7 protein, mouse; D016207:Cytokines; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D054400:Receptors, CCR7