title: Surface display of rice stripe virus NSvc2 and analysis of its membrane fusion activity.
abstract: Rice stripe virus (RSV) infects rice and is transmitted in a propagative manner by the small brown planthopper. How RSV enters an insect cell to initiate the infection cycle is poorly understood. Sequence analysis revealed that the RSV NSvc2 protein was similar to the membrane glycoproteins of several members in the family Bunyaviridae and might induce cell membrane fusion. To conveniently study the membrane fusion activity of NSvc2, we constructed cell surface display vectors for expressing Nsvc2 on the insect cell surface as the membrane glycoproteins of the enveloped viruses. Our results showed that NSvc2 was successfully expressed and displayed on the surface of insect Sf9 cells. When induced by low pH, the membrane fusion was not observed in the cells that expressed NSvc2. Additionally, the membrane fusion was also not detected when co-expressing Nsvc2 and the viral capsid protein on insect cell surface. Thus, RSV NSvc2 is probably different from the phlebovirus counterparts, which could suggest different functions. RSV might enter insect cells other than by fusion with plasma or endosome membrane.
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.
authors.forename: Shu-ling
authors.initials: SL
authors.lastname: Zhao
authors.forename: Xue-juan
authors.initials: XJ
authors.lastname: Dai
authors.forename: Jian-sheng
authors.initials: JS
authors.lastname: Liang
authors.forename: Chang-yong
authors.initials: CY
authors.lastname: Liang
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology*; D021381:Protein Transport; D018411:Spodoptera; D029364:Tenuivirus / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D014764:Viral Proteins