title: Mechanical control of morphogenesis by Fat/Dachsous/Four-jointed planar cell polarity pathway.
abstract: During animal development, several planar cell polarity (PCP) pathways control tissue shape by coordinating collective cell behavior. Here, we characterize by means of multiscale imaging epithelium morphogenesis in the Drosophila dorsal thorax and show how the Fat/Dachsous/Four-jointed PCP pathway controls morphogenesis. We found that the proto-cadherin Dachsous is polarized within a domain of its tissue-wide expression gradient. Furthermore, Dachsous polarizes the myosin Dachs, which in turn promotes anisotropy of junction tension. By combining physical modeling with quantitative image analyses, we determined that this tension anisotropy defines the pattern of local tissue contraction that contributes to shaping the epithelium mainly via oriented cell rearrangements. Our results establish how tissue planar polarization coordinates the local changes of cell mechanical properties to control tissue morphogenesis.
authors.affiliation: Polarity, Division and Morphogenesis Team, Institut Curie, CNRS UMR 3215, INSERM U934, 26 Rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: Floris
authors.initials: F
authors.lastname: Bosveld
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bonnet
authors.forename: Boris
authors.initials: B
authors.lastname: Guirao
authors.forename: Sham
authors.initials: S
authors.lastname: Tlili
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wang
authors.forename: Ambre
authors.initials: A
authors.lastname: Petitalot
authors.forename: Raphaël
authors.initials: R
authors.lastname: Marchand
authors.forename: Pierre-Luc
authors.initials: PL
authors.lastname: Bardet
authors.forename: Philippe
authors.initials: P
authors.lastname: Marcq
authors.forename: François
authors.initials: F
authors.lastname: Graner
authors.forename: Yohanns
authors.initials: Y
authors.lastname: Bellaïche
mesh_terms: D000818:Animals; D016880:Anisotropy; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D016764:Cell Polarity; D048430:Cell Shape; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D007365:Intercellular Junctions / Q000378:metabolism / Q000502:physiology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008675:Metamorphosis, Biological; D008954:Models, Biological; D009024:Morphogenesis; D009218:Myosins / Q000378:metabolism; D011679:Pupa / Q000254:growth & development / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D013909:Thorax / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; C492999:dachs protein, Drosophila; C094449:ds protein, Drosophila; C095900:fj protein, Drosophila; C472307:ft protein, Drosophila; D009218:Myosins