title: Modeling human hematopoietic cell development from pluripotent stem cells.
abstract: Understanding the steps and cues that allow hematopoietic cells to be generated during development holds great clinical as well as biological interest. Analysis of these events in mice has provided many important insights into the processes involved, but features that might be unique to humans remain challenging to elucidate because they cannot be studied directly in vivo. Human embryonic stem or induced pluripotent stem cells offer attractive in vitro alternatives to analyze the process. Here we review recent efforts to develop defined and quantitative systems to address outstanding developmental questions against a background of what we know about the development of hematopoietic cells in the fetus and derived from mouse embryonic stem cells.
authors.affiliation: Terry Fox Laboratory, British Columbia Cancer Agency and the Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Melanie D
authors.initials: MD
authors.lastname: Kardel
authors.forename: Connie J
authors.initials: CJ
authors.lastname: Eaves
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008648:Mesoderm / Q000502:physiology; D008954:Models, Biological; D039904:Pluripotent Stem Cells / Q000166:cytology*