title: Successful strategy for targeting the central nervous system using magnetic albumin nanospheres.
abstract: This study reports on the successful use of magnetic albumin nanosphere (MAN), consisting of maghemite nanoparticles hosted by albumin-based nanosphere, to target different sites within the central nervous system (CNS). Ultrastructural analysis by transmission electron microscopy (TEM) of the material collected from the mice was performed in the time window of 30 minutes up to 30 days after administration. Evidence found that the administered MAN was initially internalized and transported by erythrocytes across the blood-brain-barrier and transferred to glial cells and neuropils before internalization by neurons, mainly in the cerebellum. We hypothesize that the efficiency of MAN in crossing the BBB with no pathological alterations is due to the synergistic effect of its two main components, the iron-based nanosized particles and the hosting albumin-based nanospheres. We found that the MAN in targeting the CNS represents an important step towards the design of nanosized materials for clinical and diagnostic applications.
authors.affiliation: University of Brasília, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Electronic Microscopy, Brasília DF, 70919-970, Brazil.
authors.forename: Débora de Oliveira
authors.initials: Dde O
authors.lastname: Cintra e Silva
authors.forename: Luciana Landim Carneiro
authors.initials: LL
authors.lastname: Estevanato
authors.forename: Andreza Ribeiro
authors.initials: AR
authors.lastname: Simioni
authors.forename: Marcilene Machado de Andrade
authors.initials: MM
authors.lastname: Rodrigues
authors.forename: Bruno Marques
authors.initials: BM
authors.lastname: Lacava
authors.forename: Zulmira Guerrero Marques
authors.initials: ZG
authors.lastname: Lacava
authors.forename: Antônio Cláudio
authors.initials: AC
authors.lastname: Tedesco
authors.forename: Paulo César
authors.initials: PC
authors.lastname: Morais
authors.forename: Sônia Nair
authors.initials: SN
authors.lastname: Báo
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000737:chemistry / Q000166:cytology; D001921:Brain / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D001923:Brain Chemistry; D002417:Cattle; D002491:Central Nervous System Agents / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods; D006651:Histocytochemistry; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D002491:Central Nervous System Agents; D058185:Magnetite Nanoparticles; D012710:Serum Albumin, Bovine