title: Polysaccharide nanofibrous scaffolds as a model for in vitro skin tissue regeneration.
abstract: Tissue engineering and nanotechnology have advanced a general strategy combining the cellular elements of living tissue with sophisticated functional biocomposites to produce living structures of sufficient size and function at a low cost for clinical relevance. Xylan, a natural polysaccharide was electrospun along with polyvinyl alcohol (PVA) to produce Xylan/PVA nanofibers for skin tissue engineering. The Xylan/PVA glutaraldehyde (Glu) vapor cross-linked nanofibers were characterized by SEM, FT-IR, tensile testing and water contact angle measurements to analyze the morphology, functional groups, mechanical properties and wettability of the fibers for skin tissue regeneration. The cell-biomaterial interactions were studied by culturing human foreskin fibroblasts on Xylan/PVA Glu vapor cross-linked and Xylan/PVA/Glu blend nanofibrous scaffolds. The observed results showed that the mechanical properties (72 %) and fibroblast proliferation significantly increased up to 23 % (P < 0.05) in 48 h Glu vapor cross-linked nanofibers compared to 24 h Glu vapor cross-linked Xylan/PVA nanofibers. The present study may prove that the natural biodegradable Xylan/PVA nanofibrous scaffolds have good potential for fibroblast adhesion, proliferation and cell matrix interactions relevant for skin tissue regeneration.
authors.affiliation: Centre for Nanofibers & Nanotechnology, NUSNNI, Faculty of Engineering, National University of Singapore, Singapore.
authors.forename: R
authors.initials: R
authors.lastname: Krishnan
authors.forename: R
authors.initials: R
authors.lastname: Rajeswari
authors.forename: J
authors.initials: J
authors.lastname: Venugopal
authors.forename: S
authors.initials: S
authors.lastname: Sundarrajan
authors.forename: R
authors.initials: R
authors.lastname: Sridhar
authors.forename: M
authors.initials: M
authors.lastname: Shayanti
authors.forename: S
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005976:Glutaral; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011142:Polyvinyl Alcohol; D012038:Regeneration / Q000502:physiology*; D012879:Skin Physiological Phenomena; D017768:Skin, Artificial; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014990:Xylans / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D014990:Xylans; D011142:Polyvinyl Alcohol; D005976:Glutaral