title: [GAD67-GFP expression and co-localization with bNOS in main olfactory bulb of GAD67-GFP knock-in mouse].
abstract: OBJECTIVE
  To investigate the distribution of GAD67 and the co-localization with bNOS in the main olfactory bulb of GAD67-GFP knock-in mouse.
  
  
  METHODS
  Polymerase chain reaction was applied to identify the genotype of GAD67-GFP knock-in mouse, the animals were sacrificed and frozen sections of olfactory bulb were prepared. The Nissl-staining was performed to show an framework of the neuron in the olfactory bulb. The distribution of GAD67 and co-localization with bNOS were detected by immunofluorescence technique.
  
  
  RESULTS
  The proportion of GAD67-positive cells among DAPI-positive cells were (42.98 ± 0.92)% in glomerular layer, (23.64 ± 0.84)% in mitral cell layer and (77.75 ± 0.84)% in granule cell layer; the bNOS-positive cells mainly existed in glomerular layer and mitral cell layer, very few in granule cell layer. No co-localization of GAD67 and bNOS in granule cell layer and mitral cell layer was found, but there was dispersed distribution in glomerular layer.
  
  
  CONCLUSIONS
  GAD67-positive neurons mainly appear in glomerular layer and granule cell layer, and the bNOS is mostly expressed in glomerular layer and mitral cell layer; while the co-localization of GAD67 and bNOS only occurs in glomerular layer of olfactory bulb.
authors.affiliation: Department of Anatomy and Cell Biology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Han
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Wen-fu
authors.initials: WF
authors.lastname: Yu
authors.forename: Yi-fei
authors.initials: YF
authors.lastname: Yin
authors.forename: Sheng-xi
authors.initials: SX
authors.lastname: Wu
authors.forename: Shu-cai
authors.initials: SC
authors.lastname: Ling
mesh_terms: D000818:Animals; D055879:Gene Knock-In Techniques; D005968:Glutamate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D009830:Olfactory Bulb / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D049452:Green Fluorescent Proteins; C496320:NOS1 protein, human; D052248:Nitric Oxide Synthase Type I; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1