title: Validation of a new LC-MS/MS method for the detection and quantification of phenolic metabolites from tomato sauce in biological samples.
abstract: Tomato is a good source of bioactive molecules such as vitamin C, carotenoids, and phenolic compounds. Up to now, only a few studies have evaluated the bioavailability of phenolic compounds from tomato. This paper presents the optimization of a method for the determination of phenolics in tomato and their metabolites in human urine and plasma after ingestion of tomato sauce. The sample preparation includes a SPE step to obtain cleaner extracts for injection in the LC-MS/MS system. The mean recovery of analytes ranged from 73 to 104% in plasma and from 65 to 106% in urine, the accuracy was between 90.3 and 115.0% in urine and between 85.7 and 115.0% in plasma, and the precision coefficient of variation was <15%. The method allowed detection and quantification limits of 0.5-29 and 2.0-90 ng mL⁻¹ in urine, respectively, and 0.5-30 and 2.0-105 ng mL⁻¹ in plasma, respectively, for the same phenolic compounds.
authors.affiliation: Department of Nutrition and Food Science-XARTA-INSA, School of Pharmacy, University of Barcelona, Barcelona, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: Martínez-Huélamo
authors.forename: Sara
authors.initials: S
authors.lastname: Tulipani
authors.forename: Xavier
authors.initials: X
authors.lastname: Torrado
authors.forename: Ramón
authors.initials: R
authors.lastname: Estruch
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Lamuela-Raventós
mesh_terms: D000328:Adult; D002264:Carboxylic Acids / Q000032:analysis / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis / Q000097:blood / Q000652:urine; D005419:Flavonoids / Q000032:analysis / Q000097:blood / Q000652:urine; D057141:Food, Preserved / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D020719:Glucuronides / Q000097:blood / Q000652:urine; D006801:Humans; D018551:Solanum lycopersicum / Q000737:chemistry*; D008832:Microchemistry / Q000379:methods*; D010636:Phenols / Q000032:analysis* / Q000097:blood / Q000652:urine; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D016482:Urinalysis / Q000379:methods*
chemical_list: D002264:Carboxylic Acids; D002934:Cinnamates; D005419:Flavonoids; D020719:Glucuronides; D010636:Phenols