title: Memory B cell responses to Vibrio cholerae O1 lipopolysaccharide are associated with protection against infection from household contacts of patients with cholera in Bangladesh.
abstract: Vibrio cholerae O1 causes cholera, a dehydrating diarrheal disease. We have previously shown that V. cholerae-specific memory B cell responses develop after cholera infection, and we hypothesize that these mediate long-term protective immunity against cholera. We prospectively followed household contacts of cholera patients to determine whether the presence of circulating V. cholerae O1 antigen-specific memory B cells on enrollment was associated with protection against V. cholerae infection over a 30-day period. Two hundred thirty-six household contacts of 122 index patients with cholera were enrolled. The presence of lipopolysaccharide (LPS)-specific IgG memory B cells in peripheral blood on study entry was associated with a 68% decrease in the risk of infection in household contacts (P = 0.032). No protection was associated with cholera toxin B subunit (CtxB)-specific memory B cells or IgA memory B cells specific to LPS. These results suggest that LPS-specific IgG memory B cells may be important in protection against infection with V. cholerae O1.
authors.affiliation: International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
authors.forename: Sweta M
authors.initials: SM
authors.lastname: Patel
authors.forename: Mohammad Arif
authors.initials: MA
authors.lastname: Rahman
authors.forename: M
authors.initials: M
authors.lastname: Mohasin
authors.forename: M Asrafuzzaman
authors.initials: MA
authors.lastname: Riyadh
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Leung
authors.forename: Mohammad Murshid
authors.initials: MM
authors.lastname: Alam
authors.forename: Fahima
authors.initials: F
authors.lastname: Chowdhury
authors.forename: Ashraful I
authors.initials: AI
authors.lastname: Khan
authors.forename: Ana A
authors.initials: AA
authors.lastname: Weil
authors.forename: Amena
authors.initials: A
authors.lastname: Aktar
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nazim
authors.forename: Regina C
authors.initials: RC
authors.lastname: LaRocque
authors.forename: Edward T
authors.initials: ET
authors.lastname: Ryan
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Calderwood
authors.forename: Firdausi
authors.initials: F
authors.lastname: Qadri
authors.forename: Jason B
authors.initials: JB
authors.lastname: Harris
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology*; D001459:Bangladesh; D002648:Child; D002771:Cholera / Q000276:immunology / Q000517:prevention & control* / Q000635:transmission*; D002772:Cholera Toxin / Q000276:immunology; D005191:Family Characteristics; D005192:Family Health; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007156:Immunologic Memory; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D041241:Vibrio cholerae O1 / Q000276:immunology*; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D002772:Cholera Toxin