title: Bovine hydroxyapatite (Bio-Oss(®)) induces osteocalcin, RANK-L and osteoprotegerin expression in sinus lift of rabbits.
abstract: OBJECTIVE
  The aim of this study was to analyze and compare the expression of osteocalcin, RANK-L, osteoprotegerin, in sinus lift procedures using bovine hydroxyapatite (HA).
  
  
  METHODS
  Twenty four male rabbits underwent bilateral sinus lift procedures were distributed into two groups, according to the sinus filling material: Group 1) autogenous bone graft; and Group 2) bovine HA. All groups were sacrificed after 14 and 30 days, for microscopic and immunohistochemistry analysis.
  
  
  RESULTS
  At 14 days after surgery, discrete osteogenesis was observed in the highly vascularized granulation tissue surrounding HA particles, as well as woven bone deposition on the biomaterial surface. Following 30 days, well organized bone trabeculas were seen surrounding the HA granules presenting areas of osteogenic activity. Morphometric findings did not show remarkable differences between groups. Bovine HA induced similar osteocalcin, RANK-L, osteoprotegerin immunoexpressivity when compared to autogenous bone graft group for both periods evaluated in this setting.
  
  
  CONCLUSIONS
  Taken together, these data are consistent with the notion that HA has a similar biological behavior to autogenous bone graft in sinus lift of rabbits.
authors.affiliation: Department of Biosciences, Federal University of Sao Paulo - UNIFESP, SP, Brazil.
authors.forename: Marcelo Donizeti
authors.initials: MD
authors.lastname: Chaves
authors.forename: Leandro Soeiro
authors.initials: LS
authors.lastname: de Souza Nunes
authors.forename: Renato Victor
authors.initials: RV
authors.lastname: de Oliveira
authors.forename: Leandro Andrade
authors.initials: LA
authors.lastname: Holgado
authors.forename: Hugo Nary
authors.initials: HN
authors.lastname: Filho
authors.forename: Mariza Akemi
authors.initials: MA
authors.lastname: Matsumoto
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D015519:Bone Density / Q000502:physiology; D001857:Bone Matrix / Q000637:transplantation*; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000379:methods; D002113:Calcification, Physiologic / Q000502:physiology; D002417:Cattle; D006097:Granulation Tissue / Q000473:pathology; D008297:Male; D008903:Minerals / Q000627:therapeutic use*; D015675:Osteocalcin / Q000032:analysis*; D010012:Osteogenesis / Q000502:physiology; D053244:Osteoprotegerin / Q000032:analysis*; D053245:RANK Ligand / Q000032:analysis*; D011817:Rabbits; D059546:Sinus Floor Augmentation / Q000379:methods*; D013997:Time Factors; D014182:Transplantation, Autologous
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D008903:Minerals; D053244:Osteoprotegerin; D053245:RANK Ligand; D015675:Osteocalcin