title: Multilocus variable-number tandem-repeat analysis scheme for chlamydia felis genotyping: comparison with multilocus sequence typing.
abstract: Chlamydia felis is an important ocular pathogen in cats worldwide. A multilocus variable-number tandem-repeat analysis (MLVA) system for the detection of tandem repeats across the whole genome of C. felis strain Fe/C-56 was developed. Nine selected genetic loci were tested by MLVA in 17 C. felis isolates, including the C. felis Baker vaccine strain, and 122 clinical samples from different geographic origins. Analysis of the results identified 25 distinct C. felis MLVA patterns. In parallel, a recently described multilocus sequence typing scheme for the typing of Chlamydia was applied to 13 clinical samples with 12 different C. felis MLVA patterns. Rare sequence differences were observed. Thus, the newly developed MLVA system provides a highly sensitive high-resolution test for the differentiation of C. felis isolates from different origins that is suitable for molecular epidemiological studies.
authors.affiliation: Bacterial Zoonoses Unit, French Agency for Food, Environmental & Occupational Health Safety (ANSES), Maisons-Alfort, France. karine.laroucau@anses.fr
authors.forename: Karine
authors.initials: K
authors.lastname: Laroucau
authors.forename: Antonietta
authors.initials: A
authors.lastname: Di Francesco
authors.forename: Fabien
authors.initials: F
authors.lastname: Vorimore
authors.forename: Simon
authors.initials: S
authors.lastname: Thierry
authors.forename: Jean Luc
authors.initials: JL
authors.lastname: Pingret
authors.forename: Claire
authors.initials: C
authors.lastname: Bertin
authors.forename: Hermann
authors.initials: H
authors.lastname: Willems
authors.forename: Goran
authors.initials: G
authors.lastname: Bölske
authors.forename: Ross
authors.initials: R
authors.lastname: Harley
mesh_terms: D002689:Chlamydia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002690:Chlamydia Infections / Q000382:microbiology*; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial