title: Dietary pseudopurpurin effects on bone mineral density and bone geometry architecture in rats.
abstract: The objective of our study was to evaluate whether feeding pseudopurpurin affects bone mineral density and bone geometry architecture in rats. Pseudopurpurin was extracted, analyzed and purified using an HLPC-ESI-MS. Rats were given 0% and 0.5% pseudopurpurin powder in their diet. Femurs of rats were examined at 0.5, 1 and 2 months after pseudopurpurin feeding. Compared with rats in the group 0%, the bone mineral density, and the calcium, magnesium, zinc and manganese concentrations in the rats femur in the group 0.5% increased significantly at 1 month and 2 months after pseudopurpurin feeding. Analytical results of micro-computed tomography showed that the group 0.5% displayed an increase in the trabecular volume fraction, trabecular thickness and trabecular number of the distal femur at 1 and 2 months after pseudopurpurin feeding, and the mean thickness, inner perimeter, outer perimeter, and area of the femur diaphysis were significantly increased at 2 months after pseudopurpurin feeding compared with the group 0%. In parallel, the trabecular separation and structure model index of the distal femur were decreased, compared with the group 0% at 1 and 2 months after pseudopurpurin feeding. In the 0.5% and 0% groups, there was no damage to kidney and liver by histopathology analysis. The long-term feeding of pseudopurpurin is safe for rats. The feeding of 0.5% pseudopurpurin which has specific chemical affinities for calcium for bone improvement and level of bone mineral density, enhances the geometry architecture compared with the 0% group.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Wu
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Tie-Suo
authors.initials: TS
authors.lastname: Han
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Guo-Wen
authors.initials: GW
authors.lastname: Liu
authors.affiliation: College of Animal Food and Science Technology, Yunnan Agriculture University, Kunming 650201, China.
authors.forename: Wei-Zhong
authors.initials: WZ
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000008:administration & dosage* / Q000737:chemistry; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects; D002136:Calcium, Dietary / Q000008:administration & dosage; D004396:Coloring Agents / Q000008:administration & dosage / Q000737:chemistry; D019587:Dietary Supplements; D005260:Female; D005269:Femur / Q000033:anatomy & histology / Q000000981:diagnostic imaging / Q000187:drug effects; D006801:Humans; D010024:Osteoporosis / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar; D055114:X-Ray Microtomography
chemical_list: D000880:Anthraquinones; D002136:Calcium, Dietary; D004396:Coloring Agents; C511975:purpurin anthraquinone