title: Synthesis and assembly of conjugates bearing specific numbers of DNA strands per gold nanoparticle.
abstract: Here, we present a relatively simple, efficient, and high-yielding polymerase-based method for the synthesis of 15 nm gold nanoparticle conjugates bearing a specific number of 25 base oligonucleotide strands. We have shown that the conjugates bearing one or two oligonucleotide strands per particle, with the conjugates comprising a single complementary strand, self-assemble into nanoparticle dimers and trimers, respectively. Incubation of fully coated AuNPs, containing tens of oligonucleotide strands, with a conjugate bearing a single complementary strand leads to the formation of flower-shaped structures. The assembly of particles into nanoparticle structures shown here is a prerequisite for more complex controlled assembly of particles into three-dimensional macrostructures.
authors.affiliation: Department of Biochemistry, George S. Wise Faculty of Life Sciences and ‡The Center of Nanoscience and Nanotechnology, Tel-Aviv University , Ramat Aviv 69978, Israel.
authors.forename: Natalia
authors.initials: N
authors.lastname: Borovok
authors.forename: Elad
authors.initials: E
authors.lastname: Gillon
authors.forename: Alexander
authors.initials: A
authors.lastname: Kotlyar
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009841:Oligonucleotides; D006046:Gold; D004247:DNA; D004256:DNA Polymerase I