title: Aberrant expression of N-methylpurine-DNA glycosylase influences patient survival in malignant gliomas.
abstract: OBJECTIVE
  To examine the expression of N-methylpurine-DNA glycosylase (MPG) gene and protein in glioma samples with different WHO grades and its association with patients' survival.
  
  
  METHODS
  Immunohistochemistry assay, quantitative real-time PCR and Western blot analysis were carried out to investigate the expression of MPG gene and protein in 128 glioma and 10 non-neoplastic brain tissues.
  
  
  RESULTS
  MPG gene expression level in glioma tissues was significantly higher than that in non-neoplastic brain tissues (P < 0.001). Immunohistochemistry also showed that MPG protein was over-expressed in glioma tissues, which was consistent with the resutls of Western blot analysis. Additionally, the expression levels of MPG gene and protein both increase from grade I to grade IV glioma according to the results of real-time PCR, immunohistochemistry and western blot analysis. Moreover, the survival rate of MPG-positive patients was significantly lower than that of MPG-negative patients (P < 0.001). We further confirmed that the over-expression of MPG was a significant and independent prognostic indicator in glioma by multivariate analysis (P < 0.001).
  
  
  CONCLUSIONS
  Our data showed the over-expression of MPG gene and protein in human gliomas, and also suggested for the first time that MPG be an unfavorable independent prognostic indicator for glioma patients.
authors.affiliation: Department of Neurosurgery, The 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Ce
authors.initials: C
authors.lastname: Liu
authors.forename: Yanyang
authors.initials: Y
authors.lastname: Tu
authors.forename: Jun
authors.initials: J
authors.lastname: Yuan
authors.forename: Xinggang
authors.initials: X
authors.lastname: Mao
authors.forename: Shiming
authors.initials: S
authors.lastname: He
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Fu
authors.forename: Jianhai
authors.initials: J
authors.lastname: Zong
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000737:chemistry / Q000201:enzymology*; D016022:Case-Control Studies; D002648:Child; D045647:DNA Glycosylases / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005910:Glioma / Q000737:chemistry / Q000201:enzymology*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D045647:DNA Glycosylases; C076131:DNA-3-methyladenine glycosidase II