title: Cultivar variability of patatin biochemical characteristics: table versus processing potatoes (Solanum tuberosum L.).
abstract: Biochemical characteristics of patatin proteins purified by ion-exchange and affinity chromatography from tubers of 20 potato cultivars were studied to evaluate their genotype differences with respect to utility groups, table potato cultivars (TPCs) and processing potato cultivars (PPCs). Both groups of cultivars showed similar values of protein content in dry matter (3.98-7.39%) and of patatin relative abundance (5.40-35.40%). Three mass levels (∼40.6, 41.8, and 42.9 kDa) of purified patatins were found by MALDI-TOF MS within all cultivars. Differences among mass levels corresponding with the mass of sugar antenna (∼1.2 kDa) confirmed the previous concept of different glycosylation extentsin patatin proteins. It was showed that the individual types of patatin varying in their masses occur in the patatin family in a ratio specific for each of the cultivars, with the lowest mass type being the major one. Electrophoretic analyses demonstrated wide cultivar variability in number of patatin forms. Especially 2D-PAGE showed 17-23 detected protein spots independently on the utility group. Specific lipid acyl hydrolase (LAH) activity of purified patatins from the individual tested cultivars varied between 0.92 and 5.46 μmol/(min mg). Patatin samples within most of the TPCs exhibited higher values of specific LAH activity than samples of PPCs. It may be supposed that individual patatin forms do not have similar physiological roles.
authors.affiliation: Department of Plant Production and Agroecology, Faculty of Agriculture, University of South Bohemia, Studentská 13, 370 05 České Budějovice, Czech Republic. barta@zf.jcu.cz
authors.forename: Jan
authors.initials: J
authors.lastname: Bárta
authors.forename: Veronika
authors.initials: V
authors.lastname: Bártová
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Zdráhal
authors.forename: Ondrej
authors.initials: O
authors.lastname: Sedo
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D006031:Glycosylation; D008970:Molecular Weight; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D035281:Plant Tubers / Q000737:chemistry; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011198:Solanum tuberosum / Q000737:chemistry*; D013045:Species Specificity
chemical_list: D010940:Plant Proteins; D020033:Protein Isoforms; C043222:patatin protein, Solanum tuberosum; D002265:Carboxylic Ester Hydrolases