title: SPR sensing of bisphenol A using molecularly imprinted nanoparticles immobilized on slab optical waveguide with consecutive parallel Au and Ag deposition bands coexistent with bisphenol A-immobilized Au nanoparticles.
abstract: A slab-type optical waveguide (s_OWG)-based microfluidic SPR measurement system for bisphenol A was developed. This s_OWG possesses consecutive parallel gold and silver deposition bands in the line of plasmon flow, allowing two individual SPR signals to be independently obtained as a result of the difference in resonant reflection spectra of these metals. As a molecular recognition element, molecularly imprinted polymer nanoparticles (MIP-Np) were employed and immobilized on the surface of each of the gold and silver deposition bands. The resonant reflection spectra were measured on the MIP-Np-immobilized consecutive parallel gold and silver deposition bands coexistent with BPA-AuNp. The Ag-based SPR spectra showed a red shift (0.7 nm) when free BPA (0.1 mM) was passed over the BPA-AuNp/immobilized MIP-Np complexes formed on the s_OWG, unlike the case for the Au deposition band, while a large excess of BPA induced a blue shift due to the competitive desorption of BPA-AuNp from the immobilized MIP-Np on the s_OWG. By using the proposed detection system, binding events of other small molecules could be monitored in conjunction with the use of MIP-Np and labeled-AuNp.
authors.affiliation: Graduate School of Engineering, Kobe University, Nada-ku, Kobe, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Taguchi
authors.forename: Eri
authors.initials: E
authors.lastname: Takano
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D001559:Benzhydryl Compounds; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D055620:Optical Phenomena; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D000814:Aniline Compounds; D001559:Benzhydryl Compounds; D006046:Gold; D010636:Phenols; D012834:Silver; C009505:4,4'-diaminodiphenylmethane; C006780:bisphenol A; D000098826:Bisphenol A Compounds