title: Quantitative imaging of arsenic and its species in goat following long term oral exposure.
abstract: Severity of arsenic toxicity was reported to vary depending on its species. The present study reflects the status of different species of arsenic in goat following long-term exposure of arsenic leading to hepatic damage. The experiment was conducted with six black Bengal goats, which were administered with sodium arsenite orally at a dose rate of 2 mgkg(-1) daily for 84 days. Faeces, urine, hair and blood samples were collected from those animals at 14 days interval. Excretion of total arsenic was reduced from 56 days onwards through both faeces and urine indicating higher accumulation of arsenic in body. The speciation study revealed that urinary arsenic was mainly of organic type, whereas hair accumulated almost equal proportion of arsenite, arsenate and organo arsenicals. Goats excreted high proportion of organo arsenicals through faeces possibly due to hepatobiliary secretion of organo arsenic into the gut. Significantly elevated serum alanine aminotransferase and aspartate aminotransferase activities (p<0.05) along with histopathological changes in liver indicated hepatotoxicity. The arsenite fraction increased and organic proportion decreased in urine as the time progressed, which indicates that arsenite gets methylated in liver of goat. The study thus alluded that the toxicity of arsenic would aggravate if the animals were exposed for long time as the hepatotoxicity progressed resulting in decreased methylation and formation of organo arsenicals and decreased excretions through urine.
authors.affiliation: Dept. of Pharmacology and Toxicology, West Bengal University of Animal and Fishery Sciences, 37 K.B. Sarani, Kolkata 37, India.
authors.forename: Pabitra Hriday
authors.initials: PH
authors.lastname: Patra
authors.forename: Samiran
authors.initials: S
authors.lastname: Bandyopadhyay
authors.forename: Rakesh
authors.initials: R
authors.lastname: Kumar
authors.forename: Bakul Kumar
authors.initials: BK
authors.lastname: Datta
authors.forename: Chinmoy
authors.initials: C
authors.lastname: Maji
authors.forename: Suman
authors.initials: S
authors.lastname: Biswas
authors.forename: Jeevan Ranjan
authors.initials: JR
authors.lastname: Dash
authors.forename: Tapas Kumar
authors.initials: TK
authors.lastname: Sar
authors.forename: Samar
authors.initials: S
authors.lastname: Sarkar
authors.forename: Sanjib K
authors.initials: SK
authors.lastname: Manna
authors.forename: Animesh Kumar
authors.initials: AK
authors.lastname: Chakraborty
authors.forename: Tapan Kumar
authors.initials: TK
authors.lastname: Mandal
mesh_terms: D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001149:Arsenates / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity; D001151:Arsenic / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D018053:Arsenites / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity; D001219:Aspartate Aminotransferases / Q000097:blood; D002138:Calibration; D005234:Fatty Liver / Q000139:chemically induced / Q000473:pathology; D005260:Female; D006041:Goats; D008099:Liver / Q000473:pathology; D015203:Reproducibility of Results
chemical_list: D001149:Arsenates; D018053:Arsenites; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C015001:arsenite; D001151:Arsenic; C025657:arsenic acid