title: Towards standardization of canine STRs: a proposed nomenclature for six markers from the ISAG comparison-test panel.
abstract: Eight short tandem repeat markers included in the International Society for Animal Genetics panel of 24 loci investigated in canine comparison tests were analysed in a sample of pure-breed dogs, with the purpose of defining an allele nomenclature based on the number of repeats. A regression analysis of the raw data produced by the sequencer, coupled with the direct sequencing of selected alleles, allowed us to propose a system of nomenclature for six of the eight loci (four di-nucleotidic: AHT121, AHTh137, REN169018 and REN64E19, and two tetra-nucleotidic: FH2001 and FH2328). The remaining two loci (INU055 and FH2848) showed a pattern of fragments that did not resolve in a simple allele series. This work may be useful to establish a basis for comparing data across different laboratories for a set of validated canine markers, which can be used in population genetics, forensics and other analyses.
authors.affiliation: Department of Animal Pathology, University of Pisa, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Ciampolini
authors.forename: F
authors.initials: F
authors.lastname: Cecchi
authors.forename: A
authors.initials: A
authors.lastname: Bramante
authors.forename: M
authors.initials: M
authors.lastname: Tancredi
authors.forename: S
authors.initials: S
authors.lastname: Presciuttini
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D004285:Dogs / Q000145:classification* / Q000235:genetics*; D005787:Gene Frequency; D056426:Genetic Loci; D005819:Genetic Markers; D018895:Microsatellite Repeats; D012015:Reference Standards; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers