title: [Culture in vitro and plant regeneration of Panax japonicus].
abstract: OBJECTIVE
  To study the condition of culture in vitro and plant regeneration of Panax japonicus.
  
  
  METHODS
  Embryos, stems and leaves of P. japonicus were used as explants, effects of different hormones for callus induction and plant regeneration were studied and optimized.
  
  
  RESULTS
  The optimal way to obtain sterile explant for seeds was sterilized in 75% ethyl alcohol for 60 s then 0.1% HgCL2 for 12 min; Stems and leaves were sterilized in 75% ethyl alcohol for 15 s then 5% NaClO for 5 min. Used MS as basic medium, the optimal hormones combination for callus induction of embryos, stems and leaves were MS + 1.5 mg/L NAA + 1.5 mg/L 2, 4-D + 0.1 mg/L KT; MS + 1.5 mg/L NAA + 1.0 mg/L 2,4-D + 0.1 mg/L KT; MS + 1.5 mg/L NAA + 1.0 mg/L 2,4-D + 0.2 mg/L KT respectindy under the illumination. But under the darkness,the optimal callus induction hormones combination for embryos leaves were MS + 1.0 mg/L NAA + 1.5 mg/L 2,4-D +0.2 mg/L KT; 1.5 mg/L NAA + 1.5 mg/L 2,4-D + 0.1 mg/L KT; MS + 1.5 mg/L NAA + 1.0 mg/L 2,4-D + 0.1 mg/L KT respectivety. The optimal medium for germination was MS + 3.0 mg/L 6-BA + 1.0 mg/L GA3. The optimal medium for roots generation was MS + 1.0 mg/L 6-BA + 3.0 mg/L IBA.
  
  
  CONCLUSIONS
  We establish the system of culture in vitro and plant regeneration for P. japonicus.
authors.affiliation: School of Life Sciense, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region (MOE), Chongqing 400715, China.
authors.forename: Zheng-Wei
authors.initials: ZW
authors.lastname: Luo
authors.forename: Lai
authors.initials: L
authors.lastname: Zhang
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Lv
authors.forename: Xian-Qiang
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Jin-Wu
authors.initials: JW
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Sun
mesh_terms: D003470:Culture Media / Q000737:chemistry; D004203:Disinfection / Q000379:methods; D005894:Panax / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000254:growth & development; D018517:Plant Roots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D012038:Regeneration; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators