title: Development of a secondary antibody thio-functionalized microcantilever immunosensor and an ELISA for measuring ginsenoside Re content in the herb ginseng.
abstract: Ginsenoside Re (GRe) is a major active component of the Chinese medicinal herb ginseng,  Panax ginseng . A sensitive and specific monoclonal antibody (mAb), designated as mAb3D6, was generated with a GRe-bovine serum albumin conjugate as an immunogen. Microcantilever immunosensors (MCS), one modified with thiolated anti-GRe antibody and one modified with thiolated goat antimouse immunoglobulin G (IgG), were developed to detect the content of ginsenoside. The MCS immobilized with thiolated goat antimouse IgG had a better sensitivity than the MCS modified with thiolated anti-GRe antibody. The advantage of a secondary antibody thio-functionalized MCS was verified with the anti-paclitaxel mAb. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was also established with mAb3D6. The concentration of analyte producing 50% inhibition and the working range of icELISA were 1.20 and 0.15-16.1 ng/mL, respectively. The icELISA had a cross-reactivity of 89% with ginsenoside Rg1 and less than 3% with other ginsenosides. The icELISA and MCS with thiolated secondary antibody were applied for the determination of GRe in ginseng samples, and the results agreed well with those determined by high-performance liquid chromatography.
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Tiegui
authors.initials: T
authors.lastname: Nan
authors.forename: Shangquan
authors.initials: S
authors.lastname: Wu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhao
authors.forename: Weiming
authors.initials: W
authors.lastname: Tan
authors.forename: Zhaohu
authors.initials: Z
authors.lastname: Li
authors.forename: Qingchuan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Baomin
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D036145:Ginsenosides / Q000032:analysis* / Q000276:immunology; D006041:Goats; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D005894:Panax / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D036145:Ginsenosides; D006241:Haptens; D007074:Immunoglobulin G; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; C049864:ginsenoside Re