title: Engineering of fibrillar decorin matrices for a tissue-engineered trachea.
abstract: Decorin is a structural and functional proteoglycan (PG) residing in the complex network of extracellular matrix (ECM) proteins in many connective tissues. Depending on the protein core and the glycosaminoglycan chain, PGs support cell adhesion, migration, proliferation, differentiation, ECM assembly and growth factor binding. For applications in tissue engineering, it is crucial to develop reliable, ECM-mimicking biomaterials. Electrospinning is a suitable method for creating three-dimensional (3D), fibrillar scaffolds. While there are numerous reports on the electrospinning of proteins including collagen, to date, there are no reports on the electrospinning of PGs. In the following study, we used electrospinning to generate decorin-containing matrices for tracheal tissue engineering applications. The electrospun scaffolds were analyzed using scanning electron microscopy, atomic force microscopy, contact angle measurements and dynamic mechanical analysis. Additionally, we confirmed PG functionality with immunostaining and 1,9-dimethylmethylene blue. To determine cell-matrix-interactions, tracheal cells (hPAECs) were seeded and analyzed using an FOXJ1-antibody. Moreover, interactions of the electrospun scaffolds with immune-mediated mechanisms were analyzed in detail. To conclude, we demonstrated the feasibility of electrospinning of decorin to generate functional 3D scaffolds with low immunogenicity for hPAEC expansion. Our data suggest that these hybrid materials may be suitable as a substrate for tracheal tissue engineering.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, University Hospital (UKT) and Inter-University Centre for Medical Technology Stuttgart-Tübingen (IZST), Eberhard-Karls-University Tübingen, 72076 Tübingen, Germany.
authors.forename: Svenja
authors.initials: S
authors.lastname: Hinderer
authors.forename: Marianne
authors.initials: M
authors.lastname: Schesny
authors.forename: Alexandra
authors.initials: A
authors.lastname: Bayrak
authors.forename: Bettina
authors.initials: B
authors.lastname: Ibold
authors.forename: Martina
authors.initials: M
authors.lastname: Hampel
authors.forename: Thorsten
authors.initials: T
authors.lastname: Walles
authors.forename: Ulrich A
authors.initials: UA
authors.lastname: Stock
authors.forename: Martina
authors.initials: M
authors.lastname: Seifert
authors.forename: Katja
authors.initials: K
authors.lastname: Schenke-Layland
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D058575:Decorin / Q000276:immunology / Q000494:pharmacology* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects* / Q000378:metabolism*; D005452:Fluoresceins / Q000378:metabolism; D005780:Gelatin / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018625:Microscopy, Atomic Force; D009928:Organ Specificity / Q000187:drug effects; D011091:Polyesters / Q000494:pharmacology; D013388:Succinimides / Q000378:metabolism; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014132:Trachea / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D000906:Antibodies; D016207:Cytokines; D058575:Decorin; D005452:Fluoresceins; D011091:Polyesters; D013388:Succinimides; C016240:polycaprolactone; D005780:Gelatin