title: L-Phenylalanine catabolism and L-phenyllactic acid production by a phototrophic bacterium, Rubrivivax benzoatilyticus JA2.
abstract: A phototrophic bacterium (Rubrivivax benzoatilyticus JA2) grows at the expense of L-phenylalanine as sole source of nitrogen but not as carbon source. Near stoichiometric yields of L-phenylpyruvic acid (0.4 mM) and L-phenyllactate (0.4 mM) were observed from L-phenylalanine (0.9 mM consumed). Aminotransfarase and dehydrogenase activities involved in the formation of L-phenylpyruvic acid and L-phenyllactate were demonstrated unequivocally in Rubrivivax benzoatilyticus JA2. Growth conditions and carbon sources had an influence on L-phenyllactate production. The process yielded a maximum of 0.92 mM L-phenyllactate from L-phenylalanine (1 mM) when fructose served as carbon source for R. benzoatilyticus JA2.
authors.affiliation: Department of Plant Sciences, University of Hyderabad, Hyderabad 500046, India.
authors.forename: M Lakshmi
authors.initials: ML
authors.lastname: Prasuna
authors.forename: Md
authors.initials: M
authors.lastname: Mujahid
authors.forename: Ch
authors.initials: Ch
authors.lastname: Sasikala
authors.forename: Ch V
authors.initials: ChV
authors.lastname: Ramana
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D020562:Betaproteobacteria / Q000201:enzymology / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D052817:Phototrophic Processes; D000637:Transaminases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D007773:Lactates; C017648:3-phenyllactic acid; D010088:Oxidoreductases; D000637:Transaminases