title: A new multicolor fluorescence in situ hybridization probe set directed against human heterochromatin: HCM-FISH.
abstract: A new multicolor fluorescence in situ hybridization (mFISH) probe set is presented, and its possible applications are highlighted in 25 clinical cases. The so-called heterochromatin-M-FISH (HCM-FISH) probe set enables a one-step characterization of the large heterochromatic regions within the human genome. HCM-FISH closes a gap in the now available mFISH probe sets, as those do not normally cover the acrocentric short arms; the large pericentric regions of chromosomes 1, 9, and 16; as well as the band Yq12. Still, these regions can be involved in different kinds of chromosomal rearrangements such as translocations, insertions, inversions, amplifications, and marker chromosome formations. Here, examples are given for all these kinds of chromosomal aberrations, detected as constitutional rearrangements in clinical cases. Application perspectives of the probe set in tumors as well as in evolutionary cytogenetic studies are given.
authors.affiliation: Institute of Human Genetics, Jena University Hospital, Jena, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Bucksch
authors.forename: Monika
authors.initials: M
authors.lastname: Ziegler
authors.forename: Nadezda
authors.initials: N
authors.lastname: Kosayakova
authors.forename: Milene V
authors.initials: MV
authors.lastname: Mulatinho
authors.forename: Milene V
authors.initials: MV
authors.lastname: Mulhatino
authors.forename: Juan C
authors.initials: JC
authors.lastname: Llerena
authors.forename: Susanne
authors.initials: S
authors.lastname: Morlot
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fischer
authors.forename: Anna D
authors.initials: AD
authors.lastname: Polityko
authors.forename: Anna I
authors.initials: AI
authors.lastname: Kulpanovich
authors.forename: Michael B
authors.initials: MB
authors.lastname: Petersen
authors.forename: Britta
authors.initials: B
authors.lastname: Belitz
authors.forename: Vladimir
authors.initials: V
authors.lastname: Trifonov
authors.forename: Anja
authors.initials: A
authors.lastname: Weise
authors.forename: Thomas
authors.initials: T
authors.lastname: Liehr
authors.forename: Ahmed B
authors.initials: AB
authors.lastname: Hamid
mesh_terms: D004247:DNA / Q000032:analysis / Q000235:genetics; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D006570:Heterochromatin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D006570:Heterochromatin; D004247:DNA