title: Experimental foot-and-mouth disease virus infection in white tailed deer.
abstract: White tailed deer (Odocoileus virginianus) were inoculated with foot-and-mouth disease virus (FMDV) O UKG 11/2001 and monitored for the development of clinical signs, histopathological changes and levels of virus replication. All FMDV-infected deer developed clinical signs starting at 2 days post inoculation and characterized by an increase in body temperature, increased salivation and lesions in the mouth and on the feet. Virus spread to various tissues was determined by quantifying the amount of FMDV RNA using quantitative reverse transcriptase polymerase chain reaction. Virus or viral antigen was also detected in tissues using traditional isolation techniques, enzyme linked immunosorbent assay and immunohistochemistry. Deer-to-cattle transmission of the virus was observed in this experimental setting; however, inoculated deer were not found to become carriers of FMDV.
authors.affiliation: National Centres for Animal Disease, Canadian Food Inspection Agency, 1015 Arlington St., Winnipeg, Manitoba R3E 3M4, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Moniwa
authors.forename: C
authors.initials: C
authors.lastname: Embury-Hyatt
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: K
authors.initials: K
authors.lastname: Hole
authors.forename: A
authors.initials: A
authors.lastname: Clavijo
authors.forename: J
authors.initials: J
authors.lastname: Copps
authors.forename: S
authors.initials: S
authors.lastname: Alexandersen
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology; D002417:Cattle; D003670:Deer / Q000276:immunology / Q000821:virology*; D004195:Disease Models, Animal; D018562:Disease Transmission, Infectious; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005536:Foot-and-Mouth Disease / Q000473:pathology* / Q000635:transmission; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D007150:Immunohistochemistry / Q000662:veterinary; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014779:Virus Replication
chemical_list: D012367:RNA, Viral