title: The effect of ivermectin in seven strains of Aedes aegypti (Diptera: Culicidae) including a genetically diverse laboratory strain and three permethrin resistant strains.
abstract: Seven different strains of Aedes aegypti (L.), including a genetically diverse laboratory strain, three laboratory-selected permethrin-resistant strains, a standard reference strain, and two recently colonized strains were fed on human blood containing various concentrations of ivermectin. Ivermectin reduced adult survival, fecundity, and hatch rate of eggs laid by ivermectin-treated adults in all seven strains. The LC50 of ivermectin for adults and the concentration that prevented 50% of eggs from hatching was calculated for all strains. Considerable variation in adult survival after an ivermectin-bloodmeal occurred among strains, and all three permethrin-resistant strains were significantly less susceptible to ivermectin than the standard reference strain. The hatch rate after an ivermectin bloodmeal was less variable among strains, and only one of the permethrin-resistant strains differed significantly from the standard reference strain. Our studies suggest that ivermectin induces adult mortality and decreases the hatch rate of eggs through different mechanisms. A correlation analysis of log-transformed LC50 among strains suggests that permethrin and ivermectin cross-resistance may occur.
authors.affiliation: Colorado State University, Department of Microbiology, Immunology and Pathology, 1692 Campus Delivery, Fort Collins, CO 80523, USA. kdeus@caspercollege.edu
authors.forename: K M
authors.initials: KM
authors.lastname: Deus
authors.forename: K
authors.initials: K
authors.lastname: Saavedra-Rodriguez
authors.forename: M P
authors.initials: MP
authors.lastname: Butters
authors.forename: W C
authors.initials: WC
authors.lastname: Black
authors.forename: B D
authors.initials: BD
authors.lastname: Foy
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007305:Insecticide Resistance; D007306:Insecticides / Q000008:administration & dosage*; D007559:Ivermectin / Q000008:administration & dosage*; D007928:Lethal Dose 50; D052287:Oviparity / Q000187:drug effects; D010063:Ovum / Q000187:drug effects; D026023:Permethrin / Q000008:administration & dosage*; D013045:Species Specificity
chemical_list: D007306:Insecticides; D026023:Permethrin; D007559:Ivermectin