title: Distribution of cortical endoplasmic reticulum determines positioning of endocytic events in yeast plasma membrane.
abstract: In many eukaryotes, a significant part of the plasma membrane is closely associated with the dynamic meshwork of cortical endoplasmic reticulum (cortical ER). We mapped temporal variations in the local coverage of the yeast plasma membrane with cortical ER pattern and identified micron-sized plasma membrane domains clearly different in cortical ER persistence. We show that clathrin-mediated endocytosis is initiated outside the cortical ER-covered plasma membrane zones. These cortical ER-covered zones are highly dynamic but do not overlap with the immobile and also endocytosis-inactive membrane compartment of Can1 (MCC) and the subjacent eisosomes. The eisosomal component Pil1 is shown to regulate the distribution of cortical ER and thus the accessibility of the plasma membrane for endocytosis.
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Vendula
authors.initials: V
authors.lastname: Stradalova
authors.forename: Michaela
authors.initials: M
authors.lastname: Blazikova
authors.forename: Guido
authors.initials: G
authors.lastname: Grossmann
authors.forename: Miroslava
authors.initials: M
authors.lastname: Opekarová
authors.forename: Widmar
authors.initials: W
authors.lastname: Tanner
authors.forename: Jan
authors.initials: J
authors.lastname: Malinsky
mesh_terms: D002462:Cell Membrane / Q000502:physiology*; D002966:Clathrin / Q000502:physiology; D004705:Endocytosis; D004721:Endoplasmic Reticulum / Q000502:physiology*; D010750:Phosphoproteins / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology
chemical_list: D002966:Clathrin; C487327:PIL1 protein, S cerevisiae; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins