title: A novel flow-injection chemiluminescence method for determination of andrographolide in andrographis tablets.
abstract: A novel method for determination of andrographolide using flow-injection chemiluminescence (FI-CL) analysis is described in this paper. The chemiluminescence intensity of the solution was enhanced proportionally while the concentration of andrographolide increased. Under the selected experimental conditions, the calibration curve of andrographolide was linear within the range of 0.2 to 35.0 µg mL(-1) with a linear equation of ΔI = 23.391x (µg mL(-1)) + 34.191, R(2) = 0.9965. The detection limit (3σ) was 7.42 × 10(-2) µg mL(-1). At the case of continuous determination of andrographolide, the relative standard deviation (RSD, n =11) was less than 1.82%. The method has been successfully applied to the determination of andrographis tablets with satisfactory results.
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Jiang
authors.forename: Zaibin
authors.initials: Z
authors.lastname: Hao
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Hongyan
authors.initials: H
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Yan
mesh_terms: D000893:Anti-Inflammatory Agents / Q000032:analysis*; D002138:Calibration; D004224:Diterpenes / Q000032:analysis*; D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D013607:Tablets
chemical_list: D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D013607:Tablets; C030419:andrographolide