title: A novel bispecific single-chain antibody for ADAM17 and CD3 induces T-cell-mediated lysis of prostate cancer cells.
abstract: ADAM17 (A disintegrin and metalloproteinase 17) is a membrane-bound protease that cleaves various cell surface proteins, including cytokines and cytokine receptors. Recently it was shown that ADAM17 is highly expressed on the surface of many cancer cells, whereas normal cells express low levels of ADAM17, implying that ADAM17 is a potential immunotherapeutic target. We have generated a monoclonal antibody against human ADAM17, which recognized the membrane proximal cysteine-rich extension of the ADAM17 protein. Unlike normal cells, tumour cell lines, such as a prostate cancer cell line, pancreatic cancer cell lines, a breast cancer cell line and a non-small lung cancer cell line, expressed ADAM17 on the cell surface. Using the sequence of the antibody we generated an ADAM17-specific scFv (single-chain variable fragment) and fused this to a CD3-specific scFv to generate a bispecific T-cell engager antibody [A300E-BiTE (bispecific T-cell engager antibody)]. Specificity was demonstrated on cells in which ADAM17 was knocked down with a specific shRNA (short hairpin RNA). A300E-BiTE recognized ADAM17 and CD3 on the cell surface of tumour cells and T-cells respectively. In the presence of primary human peripheral blood mononuclear cells or human T-cells the addition of A300E-BiTE led to ADAM17-specific killing of prostate tumour cells indicating a novel strategy for the treatment of cancer.
authors.affiliation: Institute of Biochemistry, Christian-Albrechts-University of Kiel, Kiel, Germany.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Ahmad
authors.initials: A
authors.lastname: Trad
authors.forename: Anja
authors.initials: A
authors.lastname: Baumgart
authors.forename: Linda
authors.initials: L
authors.lastname: Hüske
authors.forename: Inken
authors.initials: I
authors.lastname: Lorenzen
authors.forename: Athena
authors.initials: A
authors.lastname: Chalaris
authors.forename: Joachim
authors.initials: J
authors.lastname: Grötzinger
authors.forename: Tobias
authors.initials: T
authors.lastname: Dechow
authors.forename: Jürgen
authors.initials: J
authors.lastname: Scheller
authors.forename: Stefan
authors.initials: S
authors.lastname: Rose-John
mesh_terms: D051722:ADAM Proteins / Q000276:immunology / Q000378:metabolism*; D000072198:ADAM17 Protein; D018033:Antibodies, Bispecific / Q000276:immunology / Q000494:pharmacology*; D017209:Apoptosis; D015153:Blotting, Western; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D047468:Immunoprecipitation; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008297:Male; D011471:Prostatic Neoplasms / Q000276:immunology / Q000473:pathology* / Q000628:therapy*; D012333:RNA, Messenger / Q000235:genetics; D057127:Single-Chain Antibodies / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D018033:Antibodies, Bispecific; D017252:CD3 Complex; D012333:RNA, Messenger; D057127:Single-Chain Antibodies; D051722:ADAM Proteins; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human