title: Epicatechin ameliorates ionising radiation-induced oxidative stress in mouse liver.
abstract: The current study was intended to evaluate the hepatoprotective effect of Epicatechin (EC) against radiation-induced oxidative stress, in terms of inflammation and lipid peroxidation. Swiss albino mice were administered with EC (15 mg/kg body weight) for three consecutive days before exposing them to a single dose of 5-Gy (60)Co gamma (γ) irradiation. Mice were necropsied and livers were taken for immunohistochemistry, western blot analysis and biochemical tests for the detection of markers of hepatic oxidative stress. Nuclear translocation of nuclear factor kappa B (NF-κB) and lipid peroxidation were increased whereas the activities of superoxide dismutase (SOD) and catalase (CAT), reduced glutathione (GSH) content and ferric reducing antioxidant power (FRAP) were diminished upon radiation exposure compared to control. Translocation of NF-κB from cytoplasm to nucleus and lipid peroxidation were found to be inhibited whereas an increase in SOD, CAT, GSH and FRAP was observed in the mice treated with EC prior to irradiation. Thus, pre-treatment with EC offers protection against γ-radiation induced hepatic alterations.
authors.affiliation: Department of Physiology, University of Calcutta, Kolkata, India.
authors.forename: Mahuya
authors.initials: M
authors.lastname: Sinha
authors.forename: Dipesh Kr
authors.initials: DK
authors.lastname: Das
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Manna
authors.forename: Sanjukta
authors.initials: S
authors.lastname: Datta
authors.forename: Tanusree
authors.initials: T
authors.lastname: Ray
authors.forename: Alok Kumar
authors.initials: AK
authors.lastname: Sil
authors.forename: Sanjit
authors.initials: S
authors.lastname: Dey
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002392:Catechin / Q000494:pharmacology*; D005720:Gamma Rays; D005978:Glutathione / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D021381:Protein Transport; D011833:Radiation Injuries, Experimental / Q000378:metabolism* / Q000517:prevention & control; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016328:NF-kappa B; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D002392:Catechin; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione