title: Arsenic interception by cell wall of bacteria observed with surface-enhanced Raman scattering.
abstract: The purpose of this study was to determine the interactions between arsenic (As) resistant bacteria and As, using surface-enhanced Raman scattering (SERS) and Fourier transform infrared (FTIR) spectroscopy. According to our 16S rDNA results, eight bacteria isolated from the environment can be identified to four genera (Arthrobacter, Pseudomonas, Sphingomonas, and Acinetobacter). The bacteria were separated into cell wall and protoplast in the study to assess the As(V) attack. The As(V) stress on bacteria could be identified with SERS, but not with FTIR. The bacteria in our study primarily resist As(V) through sequestration of As(V) by the cell wall. The change in SERS peaks and their relationships with cell wall suggested that As(V) mainly interacts with functional groups on the cell wall including polysaccharides and flavin derivates.
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Tian
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhuang
authors.forename: Anzhou
authors.initials: A
authors.lastname: Ma
authors.forename: Chuanyong
authors.initials: C
authors.lastname: Jing
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000633:toxicity*; D001151:Arsenic / Q000378:metabolism / Q000633:toxicity*; D001419:Bacteria / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D002473:Cell Wall / Q000187:drug effects* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004783:Environmental Microbiology; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013312:Stress, Physiological
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D001151:Arsenic