title: Fluoride-supplemented milk inhibits acid tolerance in root caries biofilms.
abstract: In this study we investigated the effect of fluoride on plaque acid tolerance. The test group consumed 200 ml of milk supplemented with 5 mg F/l as NaF once a day, the milk control group drank 200 ml of unsupplemented milk, and the no-milk control group did not consume milk in this manner. Plaque samples were taken at baseline and after 15 months. The proportion of acid-tolerant bacteria in plaque was estimated using LIVE/DEAD® BacLight™ staining after exposure to pH 3.5 for 2 h. The fluoride group showed a statistically significant decrease in plaque acid tolerance compared to baseline. This study shows that daily intake of fluoride in milk reduces plaque acid tolerance.
authors.affiliation: Department of Oral Biology, Faculty of Odontology, Malmö University, Malmö, Sweden. jessica.neilands@mah.se
authors.forename: J
authors.initials: J
authors.lastname: Neilands
authors.forename: L G
authors.initials: LG
authors.lastname: Petersson
authors.forename: D
authors.initials: D
authors.lastname: Beighton
authors.forename: G
authors.initials: G
authors.lastname: Svensäter
mesh_terms: D000143:Acids / Q000378:metabolism; D000222:Adaptation, Physiological / Q000187:drug effects*; D000818:Animals; D018441:Biofilms / Q000187:drug effects*; D002327:Cariostatic Agents / Q000008:administration & dosage*; D002463:Cell Membrane Permeability; D003773:Dental Plaque / Q000382:microbiology*; D019587:Dietary Supplements; D005260:Female; D005285:Fermentation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D008892:Milk / Q000737:chemistry*; D017213:Root Caries / Q000382:microbiology*; D012969:Sodium Fluoride / Q000008:administration & dosage*; D018709:Statistics, Nonparametric; D013291:Streptococcus / Q000502:physiology*
chemical_list: D000143:Acids; D002327:Cariostatic Agents; D012969:Sodium Fluoride