title: Thyroid hormone accelerates the differentiation of adult hippocampal progenitors.
abstract: Disrupted thyroid hormone function evokes severe physiological consequences in the immature brain. In adulthood, although clinical reports document an effect of thyroid hormone status on mood and cognition, the molecular and cellular changes underlying these behavioural effects are poorly understood. More recently, the subtle effects of thyroid hormone on structural plasticity in the mature brain, in particular on adult hippocampal neurogenesis, have come to be appreciated. However, the specific stages of adult hippocampal progenitor development that are sensitive to thyroid hormone are not defined. Using nestin-green fluorescent protein reporter mice, we demonstrate that thyroid hormone mediates its effects on hippocampal neurogenesis by influencing Type 2b and Type 3 progenitors, although it does not alter proliferation of either the Type 1 quiescent progenitor or the Type 2a amplifying neural progenitor. Thyroid hormone increases the number of doublecortin (DCX)-positive Type 3 progenitors, and accelerates neuronal differentiation into both DCX-positive immature neurones and neuronal nuclei-positive granule cell neurones. Furthermore, we show that this increase in neuronal differentiation is accompanied by a significant induction of specific transcription factors involved in hippocampal progenitor differentiation. In vitro studies using the neurosphere assay support a direct effect of thyroid hormone on progenitor development because neurospheres treated with thyroid hormone are shifted to a more differentiated state. Taken together, our results indicate that thyroid hormone mediates its neurogenic effects via targeting Type 2b and Type 3 hippocampal progenitors, and suggests a role for proneural transcription factors in contributing to the effects of thyroid hormone on neuronal differentiation of adult hippocampal progenitors.
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India.
authors.forename: R
authors.initials: R
authors.lastname: Kapoor
authors.forename: L A
authors.initials: LA
authors.lastname: Desouza
authors.forename: I N
authors.initials: IN
authors.lastname: Nanavaty
authors.forename: S G
authors.initials: SG
authors.lastname: Kernie
authors.forename: V A
authors.initials: VA
authors.lastname: Vaidya
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods / Q000706:statistics & numerical data; D002478:Cells, Cultured; D004195:Disease Models, Animal; D000089662:Doublecortin Protein; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects / Q000254:growth & development*; D006980:Hyperthyroidism / Q000097:blood / Q000139:chemically induced / Q000503:physiopathology; D007037:Hypothyroidism / Q000139:chemically induced / Q000503:physiopathology; D008297:Male; D008713:Methimazole; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D057054:Molecular Imaging / Q000379:methods; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D013972:Thyrotropin / Q000378:metabolism; D013974:Thyroxine / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis*; D014284:Triiodothyronine / Q000494:pharmacology / Q000502:physiology*; D015854:Up-Regulation
chemical_list: C000714847:Dcx protein, mouse; D000089662:Doublecortin Protein; D014157:Transcription Factors; D014284:Triiodothyronine; D008713:Methimazole; D013972:Thyrotropin; D013974:Thyroxine