title: Simultaneous measurement of quality factor and wavelength shift by phase shift microcavity ring down spectroscopy.
abstract: Optical resonant microcavities with ultra high quality factors are widely used for biosensing. Until now, the primary method of detection has been based upon tracking the resonant wavelength shift as a function of biodetection events. One of the sources of noise in all resonant-wavelength shift measurements is the noise due to intensity fluctuations of the laser source. An alternative approach is to track the change in the quality factor of the optical cavity by using phase shift cavity ring down spectroscopy, a technique which is insensitive to the intensity fluctuations of the laser source. Here, using biotinylated microtoroid resonant cavities, we show simultaneous measurement of the quality factor and the wavelength shift by using phase shift cavity ring down spectroscopy. These measurements were performed for disassociation phase of biotin-streptavidin reaction. We found that the disassociation curves are in good agreement with the previously published results. Hence, we demonstrate not only the application of phase shift cavity ring down spectroscopy to microcavities in the liquid phase but also simultaneous measurement of the quality factor and the wavelength shift for the microcavity biosensors in the application of kinetics measurements.
authors.affiliation: ECE Dept., McGill University, 3480 University Street, Montreal, Canada H3A 2A9. imran.cheema@mail.mcgill.ca
authors.forename: M Imran
authors.initials: MI
authors.lastname: Cheema
authors.forename: Simin
authors.initials: S
authors.lastname: Mehrabani
authors.forename: Ahmad A
authors.initials: AA
authors.lastname: Hayat
authors.forename: Yves-Alain
authors.initials: YA
authors.lastname: Peter
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Armani
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Kirk
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D001710:Biotin / Q000737:chemistry; D004867:Equipment Design; D007834:Lasers; D055620:Optical Phenomena; D044367:Phase Transition; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D019809:Streptavidin / Q000737:chemistry
chemical_list: D001710:Biotin; D019809:Streptavidin