title: Addressable multiregional and multifocal multiphoton microscopy based on a spatial light modulator.
abstract: Through a combination of a deflective phase-only diffractive spatial light modulator (SLM) and galvo scanners, an addressable multiregional and multifocal multiphoton microscope (AM-MMM) is developed. The SLM shapes an incoming mode-locked, near-infrared Ti:sapphire laser beam into multiple beamlet arrays with addressable shapes and sizes that match the regions of interest on the sample. Compared with conventional multifocal multiphoton microscope (MMM), AM-MMM achieves the effective use of the laser power with an increase of imaging rate and a decrease of photodamage without sacrifice of resolution.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Shao
authors.forename: Wan
authors.initials: W
authors.lastname: Qin
authors.forename: Honghai
authors.initials: H
authors.lastname: Liu
authors.forename: Junle
authors.initials: J
authors.lastname: Qu
authors.forename: Xiang
authors.initials: X
authors.lastname: Peng
authors.forename: Hanben
authors.initials: H
authors.lastname: Niu
authors.forename: Bruce Z
authors.initials: BZ
authors.lastname: Gao
mesh_terms: D000328:Adult; D005583:Fourier Analysis; D006801:Humans; D021621:Imaging, Three-Dimensional; D007834:Lasers; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods*; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000166:cytology