title: Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide.
abstract: The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.
authors.affiliation: Centre for Structural Biology & Division of Molecular Biosciences, Imperial College London, South Kensington, London SW7 2AZ, UK.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: James A
authors.initials: JA
authors.lastname: Garnett
authors.forename: Wei-chao
authors.initials: WC
authors.lastname: Lee
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Paula
authors.initials: P
authors.lastname: Salgado
authors.forename: Jonathan
authors.initials: J
authors.lastname: Taylor
authors.forename: Yingqi
authors.initials: Y
authors.lastname: Xu
authors.forename: Sebastian
authors.initials: S
authors.lastname: Lambert
authors.forename: Ernesto
authors.initials: E
authors.lastname: Cota
authors.forename: Steve
authors.initials: S
authors.lastname: Matthews
mesh_terms: D015815:Cell Adhesion Molecules / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D017666:Deuterium Oxide / Q000737:chemistry*; D036781:Fimbriae Proteins / Q000737:chemistry*; D010861:Fimbriae, Bacterial / Q000737:chemistry*; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D012477:Salmonella enteritidis / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; C404505:SefD protein, Salmonella; D036781:Fimbriae Proteins; D017666:Deuterium Oxide