title: Immunogenicity and efficacy of an anthrax/plague DNA fusion vaccine in a mouse model.
abstract: The efficacy of multi-agent DNA vaccines consisting of a truncated gene encoding Bacillus anthracis lethal factor (LFn) fused to either Yersinia pestis V antigen (V) or Y . pestis F1 was evaluated. A/J mice were immunized by gene gun and developed predominantly IgG1 responses that were fully protective against a lethal aerosolized B. anthracis spore challenge but required the presence of an additional DNA vaccine expressing anthrax protective antigen to boost survival against aerosolized Y. pestis.
authors.affiliation: Biological Defense Research Directorate, Naval Medical Research Center, Fort Detrick, MD, USA.
authors.forename: Mark T
authors.initials: MT
authors.lastname: Albrecht
authors.forename: Jim E
authors.initials: JE
authors.lastname: Eyles
authors.forename: Les W
authors.initials: LW
authors.lastname: Baillie
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Keane-Myers
mesh_terms: D000818:Animals; D000881:Anthrax / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D022122:Anthrax Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001408:Bacillus anthracis / Q000276:immunology / Q000472:pathogenicity; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology; D019470:Biolistics; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D010930:Plague / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D010931:Plague Vaccine / Q000008:administration & dosage / Q000276:immunology*; D052899:Pore Forming Cytotoxic Proteins / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013171:Spores, Bacterial / Q000276:immunology / Q000472:pathogenicity; D015996:Survival Rate; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D015010:Yersinia pestis / Q000276:immunology
chemical_list: D022122:Anthrax Vaccines; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D007074:Immunoglobulin G; C043908:LcrV protein, Yersinia; D010931:Plague Vaccine; D052899:Pore Forming Cytotoxic Proteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C030325:anthrax toxin; C086725:caf1 protein, Yersinia pestis