title: Antifungal saponins from bulbs of garlic, Allium sativum L. var. Voghiera.
abstract: A bioassay-guided phytochemical analysis of the polar extract from the bulbs of garlic, Allium sativum L., var. Voghiera, typical of Voghiera, Ferrara (Italy), allowed the isolation of ten furostanol saponins; voghieroside A1/A2 and voghieroside B1/B2, based on the rare agapanthagenin aglycone; voghieroside C1/C2, based on agigenin aglycone; and voghieroside D1/D2 and E1/E2, based on gitogenin aglycone. In addition, we found two known spirostanol saponins, agigenin 3-O-trisaccharide and gitogenin 3-O-tetrasaccharide. The chemical structures of the isolated compounds were established through a combination of extensive nuclear magnetic resonance, mass spectrometry and chemical analyses. High concentrations of two eugenol diglycosides were also found for the first time in Allium spp. The isolated compounds were evaluated for their antimicrobial activity towards two fungal species, the air-borne pathogen Botrytis cinerea and the antagonistic fungus Trichoderma harzianum.
authors.affiliation: Dipartimento di Scienza degli Alimenti, Università di Napoli Federico II, Via Università 100, I-80055 Portici, Napoli, Italy. lanzotti@unina.it
authors.forename: Virginia
authors.initials: V
authors.lastname: Lanzotti
authors.forename: Elisa
authors.initials: E
authors.lastname: Barile
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Antignani
authors.forename: Giuliano
authors.initials: G
authors.lastname: Bonanomi
authors.forename: Felice
authors.initials: F
authors.lastname: Scala
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020171:Botrytis / Q000187:drug effects; D005737:Garlic / Q000737:chemistry*; D007558:Italy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013150:Spirostans / Q000737:chemistry; D014242:Trichoderma / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D012503:Saponins; D013150:Spirostans; C012999:gitogenin