title: [A new natural polyphenol BJA32531 inhibited the proliferation and regulated miRNA expression in human HepG2 hepatocarcinoma cells].
abstract: OBJECTIVE
  To study the effect of 1,2,6-Tri-O-galloyl-beta-D-glucopyranose (BJA32531) on the miRNA expression during BJA32531-induced cytotoxicity in human HepG2 hepatocarcinoma cells.
  
  
  METHODS
  Cell proliferation was assessed using a colorimetric assay (cell counting kit-8). Apoptosis was assessed by annexin V and propidium iodide staining. The miRNA expression profile of the cancer cells was analyzed by a miRNA array and quantitative real-time PCR.
  
  
  RESULTS
  BJA32531 inhibited the cell proliferation and increased apoptosis in HepG2 cancer cells. Cellular exposure to BJA32531 influenced the miRNA expression pattern in the cells, including 19 upregulated and 85 down-regulated miRNAs in the cells. The up-regulations of let-7a and miR-10b as well as the down-regulations of miR-132 and miR-125b were verified to be consistent with the the results of the miRNA array.
  
  
  CONCLUSIONS
  Our study suggests that the mechanisms by which BJA32531 exerted the antiproliferative effects on HepG2 cancer cells may be related to its regulation of miRNA.
authors.affiliation: Southern Medical University, Guangzhou 510515, China. airt@cncbd.org.cn
authors.forename: Rui-Ting
authors.initials: RT
authors.lastname: Ai
authors.forename: Shao-Yu
authors.initials: SY
authors.lastname: Wu
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Wen
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Lv
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Wu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D031111:Balanophoraceae / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D020411:Oligonucleotide Array Sequence Analysis; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D035683:MicroRNAs; D059808:Polyphenols