title: Molecular identification and expression analysis of two distinct BPI/LBPs (bactericidal permeability-increasing protein/LPS-binding protein) from rock bream, Oplegnathus fasciatus.
abstract: We identified two cDNAs designated as RbBPI/LBP-1 and RbBPI/LBP2, respectively, which were identified by expressed sequence tag (EST) analysis of a lipopolysaccharide (LPS)-stimulated rock bream liver cDNA library. The two cDNA displayed 36.9% identity at the translated amino acid level. Despite the low level of identity between the two genes, high conservation was seen in the BPI/LBP/CETP N-terminal, LPS-binding, the proline-rich central and the BPI/LBP/CETP C-terminal domains. The full-length RbBPI/LBP-1 cDNA (1945 bp) contained an open reading frame (ORF) of 1431 bp encoding 476 amino acids. The full-length RbBPI/LBP-2 cDNA was 2652 bp in length and contained an ORF of 1422 bp encoding 473 amino acids. RbBPI/LBP-1 was significantly expressed in the spleen, liver, intestine and gill. On the other hand, RbBPI/LBP-2 showed significant expression in the kidney, peripheral blood leukocytes, and spleen. Real-time RT-PCR was used to examine RbBPI/LBP-1 and RbBPI/LBP-2 mRNA expression in kidney under conditions of bacterial and viral challenge. Experimental infection of rock bream with Streptococcus iniae, Edwardsiella tarda, and red sea bream iridovirus resulted in significant increases in RbBPI/LBP-1 and RbBPI/LBP-2 mRNA levels in the kidneys, however, the increases in transcription was seen at different time points.
authors.affiliation: Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University, Tongyeong, Gyeongnam, Republic of Korea.
authors.forename: Ju-Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Lena
authors.initials: L
authors.lastname: Gerwick
authors.forename: Chan-Il
authors.initials: CI
authors.lastname: Park
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004266:DNA Virus Infections / Q000378:metabolism / Q000662:veterinary; D020609:Edwardsiella tarda; D004756:Enterobacteriaceae Infections / Q000378:metabolism / Q000662:veterinary; D005393:Fish Diseases / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017994:Iridovirus; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010473:Perciformes / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013290:Streptococcal Infections / Q000378:metabolism / Q000662:veterinary; D013291:Streptococcus; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D000099231:Lipopolysaccharide-Binding Protein; C021623:bactericidal permeability increasing protein