title: [Application of an integrated omics analysis for the discovery of biomarkers for osteosarcoma].
abstract: OBJECTIVE
  To apply an integrated omics analysis so as to discover potential biomarkers for an early diagnosis of osteosarcoma.
  
  
  METHODS
  Gene chip and surface-enhanced laser desorption/ionization-time of flight-mass spectra (SELDI-TOF-MS) were employed to screen the marker genes and serum markers of osteosarcoma respectively. The associations of potential biomarkers from the SELDI data and microarray analysis were further inferred by Link-test to identify stable and specific diagnostic biomarkers.
  
  
  RESULTS
  A total of 653 pre-biomarkers were found in osteosarcoma cells. And six differentially expressed protein peaks with strong statistical significances were detected by SELDI-TOF-MS in the patient's serum samples. Thirteen potential biomarkers for an early diagnosis of osteosarcoma were screened by Link-test.
  
  
  CONCLUSIONS
  As an effective method of identifying osteosarcoma biomarkers, the integrated omics analysis may serve as a monitoring platform for an early diagnosis of osteosarcoma.
authors.affiliation: Department of Orthopedics, Affiliated No.10 People's Hospital, Tongji University, Shanghai 200072, China. litrue2004@yahoo.com.cn
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Li
authors.forename: Zheng-dong
authors.initials: ZD
authors.lastname: Cai
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Ma
authors.forename: Meng-xiong
authors.initials: MX
authors.lastname: Sun
authors.forename: Jian
authors.initials: J
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor; D001859:Bone Neoplasms / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012516:Osteosarcoma / Q000097:blood / Q000175:diagnosis*; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor