title: Increased cellular distribution of vimentin and Ret in the cingulum induced by developmental hypothyroidism in rat offspring maternally exposed to anti-thyroid agents.
abstract: To elucidate target molecules of white matter development responding to hypothyroidism, global gene expression profiling of cerebral white matter from male rat offspring was performed after maternal exposure to anti-thyroid agents, 6-propyl-2-thiouracil and methimazole, on postnatal day 20. Genes involved in central nervous system development commonly up- or down-regulated among groups treated with anti-thyroid agents. Immunohistochemical distributions of vimentin, Ret proto-oncogene (Ret), deleted in colorectal cancer protein (DCC), and Claudin11 (Cld11) were examined based on the gene expression profile. Immunoreactive cells for vimentin and Ret in the cingulum, and the immunoreactive intensity of Cld11 and DCC in whole white matter were increased by treatment with anti-thyroid agents. Immunoreactive cells for vimentin and Ret were immature astrocytes and oligodendrocytes, respectively. Thus, immunoreactive cells for vimentin and Ret may be quantitatively measurable targets of developmental hypothyroidism in white matter.
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Fujimoto
authors.forename: Gye-Hyeong
authors.initials: GH
authors.lastname: Woo
authors.forename: Kaoru
authors.initials: K
authors.lastname: Inoue
authors.forename: Katsuhide
authors.initials: K
authors.lastname: Igarashi
authors.forename: Jun
authors.initials: J
authors.lastname: Kanno
authors.forename: Masao
authors.initials: M
authors.lastname: Hirose
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Nishikawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Shibutani
mesh_terms: D000818:Animals; D013956:Antithyroid Agents / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002465:Cell Movement; D005260:Female; D020869:Gene Expression Profiling; D007037:Hypothyroidism / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008431:Maternal-Fetal Exchange; D008713:Methimazole / Q000494:pharmacology*; D009474:Neurons; D011247:Pregnancy; D011441:Propylthiouracil / Q000494:pharmacology*; D051096:Proto-Oncogene Proteins c-ret / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D046888:Tissue Array Analysis; D014746:Vimentin / Q000235:genetics* / Q000378:metabolism
chemical_list: D013956:Antithyroid Agents; D014746:Vimentin; D008713:Methimazole; D011441:Propylthiouracil; D051096:Proto-Oncogene Proteins c-ret; C495001:Ret protein, rat