title: Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films.
abstract: Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110 °C, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v).
authors.affiliation: Food and Nutrition Department, National Institute of Health Dr Ricardo Jorge, Lisbon, Portugal. anateress@gmail.com
authors.forename: Ana
authors.initials: A
authors.lastname: Sanches-Silva
authors.forename: Tiago
authors.initials: T
authors.lastname: Ribeiro
authors.forename: Tânia G
authors.initials: TG
authors.lastname: Albuquerque
authors.forename: Perfecto
authors.initials: P
authors.lastname: Paseiro
authors.forename: Raquel
authors.initials: R
authors.lastname: Sendón
authors.forename: Ana Bernaldo
authors.initials: AB
authors.lastname: de Quirós
authors.forename: Jaime
authors.initials: J
authors.lastname: López-Cervantes
authors.forename: Dalia I
authors.initials: DI
authors.lastname: Sánchez-Machado
authors.forename: Herlinda Soto
authors.initials: HS
authors.lastname: Valdez
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Angulo
authors.forename: Goizane P
authors.initials: GP
authors.lastname: Aurrekoetxea
authors.forename: Helena S
authors.initials: HS
authors.lastname: Costa
mesh_terms: D048271:Chitosan / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005285:Fermentation; D005944:Glucosamine / Q000032:analysis*; D007220:Industrial Waste / Q000032:analysis; D012758:Shellfish / Q000032:analysis*
chemical_list: D007220:Industrial Waste; D048271:Chitosan; D005944:Glucosamine