title: DE-Cadherin regulates unconventional Myosin ID and Myosin IC in Drosophila left-right asymmetry establishment.
abstract: In bilateria, positioning and looping of visceral organs requires proper left-right (L/R) asymmetry establishment. Recent work in Drosophila has identified a novel situs inversus gene encoding the unconventional type ID myosin (MyoID). In myoID mutant flies, the L/R axis is inverted, causing reversed looping of organs, such as the gut, spermiduct and genitalia. We have previously shown that MyoID interacts physically with β-Catenin, suggesting a role of the adherens junction in Drosophila L/R asymmetry. Here, we show that DE-Cadherin co-immunoprecipitates with MyoID and is required for MyoID L/R activity. We further demonstrate that MyoIC, a closely related unconventional type I myosin, can antagonize MyoID L/R activity by preventing its binding to adherens junction components, both in vitro and in vivo. Interestingly, DE-Cadherin inhibits MyoIC, providing a protective mechanism to MyoID function. Conditional genetic experiments indicate that DE-Cadherin, MyoIC and MyoID show temporal synchronicity for their function in L/R asymmetry. These data suggest that following MyoID recruitment by β-Catenin at the adherens junction, DE-Cadherin has a twofold effect on Drosophila L/R asymmetry by promoting MyoID activity and repressing that of MyoIC. Interestingly, the product of the vertebrate situs inversus gene inversin also physically interacts with β-Catenin, suggesting that the adherens junction might serve as a conserved platform for determinants to establish L/R asymmetry both in vertebrates and invertebrates.
authors.affiliation: Institute of Biology Valrose, University of Nice Sophia-Antipolis, UMR7277-CNRS, UMR1091 INSERM, Parc Valrose, 06108 Nice Cedex 2, France.
authors.forename: Astrid G
authors.initials: AG
authors.lastname: Petzoldt
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Coutelis
authors.forename: Charles
authors.initials: C
authors.lastname: Géminard
authors.forename: Pauline
authors.initials: P
authors.lastname: Spéder
authors.forename: Magali
authors.initials: M
authors.lastname: Suzanne
authors.forename: Delphine
authors.initials: D
authors.lastname: Cerezo
authors.forename: Stéphane
authors.initials: S
authors.lastname: Noselli
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D024461:Myosin Type I / Q000235:genetics / Q000378:metabolism; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D015820:Cadherins; D029721:Drosophila Proteins; C513226:Myo31DF protein, Drosophila; D051176:beta Catenin; C534574:myo61F protein, Drosophila; C501889:shg protein, Drosophila; D024461:Myosin Type I; D009218:Myosins