title: [Cysteinyl leukotriene receptor 1 is involved in rotenone-induced injury of PC12 cells].
abstract: OBJECTIVE
  To investigate whether cysteinyl leukotriene receptor 1 (CysLT₁ receptor) is involved in rotenone-induced injury of PC12 cells.
  
  
  METHODS
  After 24 h treatment with rotenone or with rotenone and the CysLT₁ receptor antagonist montelukast, PC12 cell viability was determined by the colorimetric MTT reduction assay. After PC12 cells were treated with various concentrations of rotenone for 24 h or with 3 μmol/L rotenone for various durations, the expression of CysLT(1) receptor was determined by Western blotting, and its intracellular distribution was detected by immunocytochemistry.
  
  
  RESULTS
  Rotenone (0.3-30 μmol/L) induced PC12 cell injury; this injury was significantly attenuated by montelukast at 1 and 5 μmol/L.The expression of CysLT(1) receptor increased after rotenone treatment at 1-10 μmol/L, or at 3 μmol/L for 3 and 24 h. Rotenone caused concentration-and time-dependent translocation of CysLT₁ receptor from the nucleus to the cytosol.
  
  
  CONCLUSIONS
  Cysteinyl leukotriene receptor 1 is involved in rotenone-induced injury of PC12 cells.
authors.affiliation: Department of Pharmacology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Shu-ying
authors.initials: SY
authors.lastname: Yu
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.forename: Xia-yan
authors.initials: XY
authors.lastname: Zhang
authors.forename: Xaio-yan
authors.initials: XY
authors.lastname: Zhang
authors.forename: Yan-fang
authors.initials: YF
authors.lastname: Wang
authors.forename: Li-hui
authors.initials: LH
authors.lastname: Zhang
authors.forename: Yun-bi
authors.initials: YB
authors.lastname: Lu
authors.forename: Er-qing
authors.initials: EQ
authors.lastname: Wei
mesh_terms: D000818:Animals; D016716:PC12 Cells; D051381:Rats; D018077:Receptors, Leukotriene / Q000378:metabolism / Q000502:physiology*; D012402:Rotenone / Q000633:toxicity*
chemical_list: D018077:Receptors, Leukotriene; D012402:Rotenone; C083169:leukotriene D4 receptor