title: A new method for the production of gelatin microparticles for controlled protein release from porous polymeric scaffolds.
abstract: Tissue engineering using scaffolds and growth factors is a crucial approach in bone regeneration and repair. The combination of bioactive agents carrying microparticles with porous scaffolds can be an efficient solution when controlled release of bio-signalling molecules is required. The present study was based on a recent approach using a biodegradable scaffold and protein-loaded microparticles produced in an innovative manner in which protein loss is minimized during the loading process. Bovine serum albumin (BSA)-loaded gelatin microparticles were obtained by grinding freeze-dried membranes of gelatin and BSA. Porous scaffolds (250-355 µm pore size) produced from a polyactide (PLLA) and polycaprolactone (PCL) blend by salt leaching/supercritical CO₂ methods were used for the experiments. Gelatin microparticles containing three different BSA amounts were incorporated into the porous scaffolds by using a surfactant. In vitro release profiles showed up to 90% protein loading efficiency. This novel method appears to be an effective approach for producing particles that can minimize protein loss during the loading process.
authors.affiliation: Hacettepe University, Chemical Engineering Department and Bioengineering Division- Center for Bioengineering and Biyomedtek, Beytepe, Ankara, Turkey.
authors.forename: Asya
authors.initials: A
authors.lastname: Ozkizilcik
authors.forename: Kadriye
authors.initials: K
authors.lastname: Tuzlakoglu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001842:Bone and Bones / Q000473:pathology; D002417:Cattle; D016503:Drug Delivery Systems; D005780:Gelatin / Q000737:chemistry*; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry; D007700:Kinetics; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D036341:Intercellular Signaling Peptides and Proteins; D011091:Polyesters; D011108:Polymers; D011506:Proteins; D013501:Surface-Active Agents; C016240:polycaprolactone; D012710:Serum Albumin, Bovine; C033616:poly(lactide); D005780:Gelatin