title: Is there a role for prostate tumour overexpressed-1 in the diagnosis of HGPIN and of prostatic adenocarcinoma? A comparison with alpha-methylacyl CoA racemase.
abstract: Prostate Tumour Overexpressed-1 (PTOV1) was recently identified as a novel gene and protein during a differential display screening for genes overexpressed in prostate cancer (PCa). Alpha-Methyl-CoA racemase (AMACR) mRNA was identified as being overexpressed in PCa. PTOV1 and racemase were immunohistochemically  evaluated  in  PCa,  high-grade  prostatic  intraepithelial  neoplasia  (HGPIN), atrophy and normal-looking epithelium (NEp) in 20 radical prostatectomies (RPs) with pT2a Gleason score 6 prostate cancer with the aim of analyzing the differences in marker expression between PTOV1 and AMACR.  The level of expression of PTOV1 and AMACR increased from NEp and atrophy through HGPIN, away from and adjacent to prostate cancer, to PCa. With the ROC curve analysis the overall accuracy in distinguishing PCa vs HGPIN away from and adjacent to cancer was higher for AMACR than for PTOV1. In conclusion, AMACR can be considered a more accurate marker than PTOV1 in the identification of HGPIN and of PCa. However, PTOV1 may aid in the diagnosis of PCa, at least to supplement AMACR as another positive marker of carcinoma and to potentially increase diagnostic accuracy.
authors.affiliation: Section of Pathological Anatomy, Polytechnic University of the Marche Region, School of  Medicine, United Hospitals, Ancona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Scarpelli
authors.forename: R
authors.initials: R
authors.lastname: Mazzucchelli
authors.forename: F
authors.initials: F
authors.lastname: Barbisan
authors.forename: A
authors.initials: A
authors.lastname: Santinelli
authors.forename: A
authors.initials: A
authors.lastname: Lopez-Beltran
authors.forename: L
authors.initials: L
authors.lastname: Cheng
authors.forename: R
authors.initials: R
authors.lastname: Montironi
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis*; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis* / Q000502:physiology; D019048:Prostatic Intraepithelial Neoplasia / Q000175:diagnosis*; D011471:Prostatic Neoplasms / Q000175:diagnosis*; D012372:ROC Curve; D019998:Racemases and Epimerases / Q000032:analysis*; D048868:Adaptor Proteins, Signal Transducing
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D019998:Racemases and Epimerases; C425183:PTOV1 protein, human; D048868:Adaptor Proteins, Signal Transducing; C108352:alpha-methylacyl-CoA racemase