title: Synthesis of a 3-deoxy-D-manno-octulosonic acid (KDO) building block from D-glucose via fermentation.
abstract: Herein we report the first synthesis of a 3-deoxy-D-manno-octulosonic acid (KDO) building block starting from glucose through pathway engineering of Escherichia coli and subsequent chemical modifications to provide an alternative method to produce KDO, found in plant and bacterial oligosaccharides.
authors.affiliation: Department of Chemistry, Iowa State University, Ames, IA 50011-3111, USA.
authors.forename: Gulden
authors.initials: G
authors.lastname: Camci-Unal
authors.forename: Rahman M
authors.initials: RM
authors.lastname: Mizanur
authors.forename: Yonghai
authors.initials: Y
authors.lastname: Chai
authors.forename: Nicola L B
authors.initials: NL
authors.lastname: Pohl
mesh_terms: D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism*; D013400:Sugar Acids / Q000378:metabolism*
chemical_list: D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; D005947:Glucose