title: Effect of the expression of matrix metalloproteases and their tissue inhibitors on survival of patients with resectable colorectal cancer.
abstract: BACKGROUND
  Matrix metalloproteases (MMPs) and their tissue inhibitors (TIMPs) are of crucial importance in the degradation of the stromal connective tissue and basement membrane components. Study of the behavior of these components might help to predict the aggressiveness of tumors.
  
  
  OBJECTIVE
  To evaluate the expression and clinical relevance of MMPs and TIMPs for patients with resectable colorectal carcinoma.
  
  
  METHODS
  An immunohistochemical study was performed using tissue arrays and specific antibodies against MMPs-1, 2, 7, 9, 11, 13, and 14, and TIMPs-1, 2 and 3. Determinations were performed in cancer specimens from 104 patients with resectable colorectal cancer. The minimum period of follow-up was 12.5 years for patients without recurrence. To identify specific groups of tumors with distinct expression profiles, the data were analyzed by unsupervised hierarchical cluster analysis.
  
  
  RESULTS
  Expression of MMP-11 by fibroblasts and MMP-13 by tumor cells were associated with poor prognosis. The dendrogram revealed first-order division of tumors into two distinct MMP/TIMP molecular profiles, designated group 1 (n = 50) and group 2 (n = 54). Group 2 was characterized by significantly higher expression of MMP-1, 11, and 13, and TIMP-3.
  
  
  CONCLUSIONS
  Our results emphasize the prognostic value of MMP-11 and 13 expression in colorectal cancer.
authors.affiliation: Unidad de Investigación, Hospital de Jove, Avda. Eduardo Castro s/n, 33920 Gijón, Asturias, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: González
authors.forename: Noemí
authors.initials: N
authors.lastname: Eiró
authors.forename: Luis O
authors.initials: LO
authors.lastname: González
authors.forename: Alejandro
authors.initials: A
authors.lastname: Andicoechea
authors.forename: Esther
authors.initials: E
authors.lastname: Barbón
authors.forename: José L
authors.initials: JL
authors.lastname: García-Muñiz
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Vizoso
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002277:Carcinoma / Q000201:enzymology* / Q000401:mortality; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000401:mortality; D005260:Female; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D013030:Spain / Q000453:epidemiology; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases