title: Absorption of aminoethyl cysteine ketimine decarboxylated dimer in mice: effect on plasma antioxidant potential.
abstract: Aminoethylcysteine ketimine decarboxylated dimer (AECK-DD) is a natural compound with antioxidant properties of a new family of sulfur-containing amino acids. It has been detected in human urine and plasma, in mammalian cerebellum, and in dietary vegetables. In this study, we first demonstrate the absorption of AECK-DD in mice from AECK-DD-supplemented diet, using both liquid chromatography with electrochemical detection and gas chromatography coupled with mass spectrometry. AECK-DD circulates in the plasma of supplemented mice at a micromolar concentration and is incorporated in liver tissue. The absorption of AECK-DD is dose dependent. The dehydrogenation product of AECK-DD was also identified in plasma and liver of mice fed the AECK-DD-supplemented diet. A significant increase in plasma antioxidant potential was measured in mice fed AECK-DD-supplemented diet with respect to mice fed the control diet. These results demonstrate for the first time the absorption of AECK-DD from diet and the physiological relevance of this compound through its antioxidant action in vivo.
authors.affiliation: National Institute for Food and Nutrition Research, Via Ardeatina 546, 00178, Rome, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Piazzon
authors.forename: Alberto
authors.initials: A
authors.lastname: Macone
authors.forename: Rosa Marina
authors.initials: RM
authors.lastname: Matarese
authors.forename: Alberto
authors.initials: A
authors.lastname: Finamore
authors.forename: Mirella
authors.initials: M
authors.lastname: Nardini
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D007408:Intestinal Absorption; D051379:Mice; D008807:Mice, Inbred BALB C; D009025:Morpholines / Q000097:blood / Q000378:metabolism*; D011897:Random Allocation; D014675:Vegetables / Q000737:chemistry
chemical_list: C086349:5,9a-dihydro-5-oxopyrrolo(2,1-c-3,4-b')dithiomorpholine; D000975:Antioxidants; D009025:Morpholines