title: Unhealthy diet and ultrafine carbon black particles induce senescence and disease associated phenotypic changes.
abstract: Diet and pollution are environmental factors known to compromise "healthy aging" of the cardiovascular and respiratory systems. The molecular consequences of this permanent burden in these cells are still unknown. Therefore, this study investigates the impact of unhealthy diet on aging-related signaling pathways of human, primary cardiovascular cells and of airborne particles on lung epithelial and human endothelial cells. Nutrition health reports have shown that the diet in industrialized countries contains more than 100mg/dl low density lipoprotein (LDL) and a high fraction of added sugars, especially fructose. Several studies demonstrated that ultrafine particles can enter the circulation and thus may interact with endothelial cells directly. Both, dietary compounds and pollution derived particles, have been shown to increase the risk for cardiovascular diseases. To simulate an unhealthy diet, we supplemented cell culture media of human primary endothelial cells, smooth muscle cells and cardiomyocytes with LDL and replaced 1/3 of glucose with fructose. We observed hypertrophy in cardiomyocytes, enhanced proliferation in smooth muscle cells and increased senescence, loss of endothelial nitric oxide synthase and increased nuclear FoxO3A in endothelial cells. With respect to pollution we have used ultrafine carbon black particles (ufCB), one of the major constituents of industrial and exhaust emissions, in concentrations our lungs and vessels are constantly exposed to. These concentrations of ufCB increased reactive oxygen species in lung epithelial and vascular endothelial cells and reduced the S-NO content, a marker for NO-bioavailability, in endothelial cells. NO increases activation of Telomerase Reverse Transcriptase (TERT), an enzyme essential for telomere maintenance. TERT is required for proper endothelial cell function and is inactivated by Src kinase under conditions of oxidative stress. ufCB significantly increased Src kinase activation and reduced Telomerase activity in endothelial and lung epithelial cells. As a consequence, ufCB increased senescence of endothelial cells. To investigate whether ufCB show also effects in vivo, we instilled ufCB in concentrations not inducing inflammation into mice. Indeed, eNOS expression was reduced in the abdominal aorta of animals treated with ufCB. Thus, a combination of fructose and LDL in the diet and ufCB, as a major constituent of air pollution, seem to accelerate respiratory and cardiovascular cellular changes, which may compromise "healthy aging" and can lead to cardiovascular and pulmonary diseases.
authors.affiliation: Molecular Aging Research, IUF - Leibniz Research Institute for Environmental Medicine at University of Duesseldorf gGmbH, 40225 Duesseldorf, Germany. Nicole.Buechner@uni-duesseldorf.de
authors.forename: Nicole
authors.initials: N
authors.lastname: Büchner
authors.forename: Niloofar
authors.initials: N
authors.lastname: Ale-Agha
authors.forename: Sascha
authors.initials: S
authors.lastname: Jakob
authors.forename: Ulrich
authors.initials: U
authors.lastname: Sydlik
authors.forename: Kerstin
authors.initials: K
authors.lastname: Kunze
authors.forename: Klaus
authors.initials: K
authors.lastname: Unfried
authors.forename: Joachim
authors.initials: J
authors.lastname: Altschmied
authors.forename: Judith
authors.initials: J
authors.lastname: Haendeler
mesh_terms: D000393:Air Pollutants / Q000494:pharmacology / Q000633:toxicity; D000818:Animals; D001012:Aorta, Abdominal / Q000201:enzymology; D049109:Cell Proliferation / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects* / Q000502:physiology; D008078:Cholesterol, LDL / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D004032:Diet; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005632:Fructose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000096:biosynthesis; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis; D010316:Particle Size; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D053260:Soot / Q000494:pharmacology* / Q000633:toxicity; D019098:Telomerase / Q000096:biosynthesis; D019061:src-Family Kinases / Q000096:biosynthesis
chemical_list: D000393:Air Pollutants; D008078:Cholesterol, LDL; D003470:Culture Media; D017382:Reactive Oxygen Species; D053260:Soot; D005632:Fructose; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496315:Nos3 protein, mouse; D019061:src-Family Kinases; D019098:Telomerase; D005947:Glucose