title: Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad.
abstract: We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenase-encoding genes.
authors.affiliation: The Swette Center for Environmental Biotechnology, The Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
authors.forename: Erica M
authors.initials: EM
authors.lastname: Hartmann
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Badalamenti
authors.forename: Rosa
authors.initials: R
authors.lastname: Krajmalnik-Brown
authors.forename: Rolf U
authors.initials: RU
authors.lastname: Halden
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods; D001711:Biotransformation; D004147:Dioxins / Q000378:metabolism*; D005798:Genes, Bacterial; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010957:Plasmids; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012988:Soil Microbiology; D020578:Sphingomonas / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D004147:Dioxins; C020887:dibenzo(1,4)dioxin