title: Proteomic study and marker protein identification of Caenorhabditis elegans lipid droplets.
abstract: Lipid droplets (LDs) are a neutral lipid storage organelle that is conserved across almost all species. Many metabolic syndromes are directly linked to the over-storage of neutral lipids in LDs. The study of LDs in Caenorhabditis elegans (C. elegans) has been difficult because of the lack of specific LD marker proteins. Here we report the purification and proteomic analysis of C. elegans lipid droplets for the first time. We identified 306 proteins, 63% of these proteins were previously known to be LD-proteins, suggesting a similarity between mammalian and C. elegans LDs. Using morphological and biochemical analyses, we show that short-chain dehydrogenase, DHS-3 is almost exclusively localized on C. elegans LDs, indicating that it can be used as a LD marker protein in C. elegans. These results will facilitate further mechanistic studies of LDs in this powerful genetic system, C. elegans.
authors.affiliation: Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Huimin
authors.initials: H
authors.lastname: Na
authors.forename: Zhenglong
authors.initials: Z
authors.lastname: Liu
authors.forename: Shuyan
authors.initials: S
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Xue
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Pu
authors.forename: Gong
authors.initials: G
authors.lastname: Peng
authors.forename: Xun
authors.initials: X
authors.lastname: Huang
authors.forename: Fuquan
authors.initials: F
authors.lastname: Yang
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Xie
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Pingyong
authors.initials: P
authors.lastname: Xu
authors.forename: Guangshuo
authors.initials: G
authors.lastname: Ou
authors.forename: Shaobing O
authors.initials: SO
authors.lastname: Zhang
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D015153:Blotting, Western; D044926:Butyryl-CoA Dehydrogenase / Q000032:analysis*; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000032:analysis*; D022162:Cytoplasmic Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D013058:Mass Spectrometry; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D029742:Caenorhabditis elegans Proteins; D008055:Lipids; D020543:Proteome; D044926:Butyryl-CoA Dehydrogenase