title: Ability of tetraploid rat blastocysts to support fetal development after complementation with embryonic stem cells.
abstract: This study was undertaken to generate rat offspring via tetraploid blastocyst complementation with embryonic stem (ES) cells. Tetraploid blastocysts were prepared by electrofusion of blastomeres from two-cell stage embryos, and subsequent in vivo culture for 4 days. Microinjection into the tetraploid blastocoel of an inner cell mass isolated by immunosurgery resulted in the generation of rat offspring, suggesting the successful contribution of tetraploid blastocysts to their placenta. Tetraploid blastocyst complementation was attempted with a total of 4 ES cell lines (2 lines of female karyotype and 2 lines of male karyotype). In the rESWIv-3i-5 (XX) cell line, normal-sized fetuses with heartbeats were harvested on E11.5 (12.1%), E12.5 (9.5%), and E13.5 (9.1%), but no viable fetuses were detected on E14.5. Similarly, use of the rESWIv-3i-1 (XX) cell line resulted in no viable fetus production on E14.5. Using the rESBLK2i-1 (XY) cell line, viable fetuses were harvested not only on E11.5-E13.5 (2.6-5.5%), but also on E14.5 (3.0%). The transfer of a total of 487 tetraploid blastocysts complemented with rESBLK2i-1 cells resulted in 256 implantation sites (52.6%) on E21.5, but no viable offspring was detected. Use of the rESBLK2i-1/huKO (XY) cell line also resulted in no viable offspring production on E21.5. Analyses of the methylation pattern in differentially methylated regions and transcript level of genes that are imprinted in mice (H19, Meg3, Igf2r, Peg5, and Peg10) in the E14.5 conceptuses indicated a marked difference between the ES cell-derived and control normal fetuses, but not between the tetraploid and control diploid placenta.
authors.affiliation: Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi, Japan. mhirarin@nips.ac.jp
authors.forename: Masumi
authors.initials: M
authors.lastname: Hirabayashi
authors.forename: Chihiro
authors.initials: C
authors.lastname: Tamura
authors.forename: Makoto
authors.initials: M
authors.lastname: Sanbo
authors.forename: Teppei
authors.initials: T
authors.lastname: Goto
authors.forename: Megumi
authors.initials: M
authors.lastname: Kato-Itoh
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Nakauchi
authors.forename: Shinichi
authors.initials: S
authors.lastname: Hochi
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D002459:Cell Fusion; D002460:Cell Line; D047108:Embryonic Development; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D005260:Female; D047109:Fetal Development; D008297:Male; D008845:Microinjections; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D055647:Rats, Transgenic; D057891:Tetraploidy