title: Induction of apoptosis by a combination of paclitaxel and carboplatin in the presence of hyperthermia.
abstract: OBJECTIVE
  To study enhancing effects of paclitaxel in the thermochemotherapy of osteosarcoma cell lines and related mechanisms.
  
  
  METHODS
  Paclitaxel and carboplatin were used alone or jointly on OS732 cell lines in the presence of hyperthermia. Inhibition of proliferation was measured by MTT assay and cellular changes were assessed with inverted phase contrast and fluorescence microscopy. Apoptosis was analyzed with flow cytometry (FCM) and Fas expression by immunocytochemistry.
  
  
  RESULTS
  At 43 degrees C, one hour after the application of 10 μg/ml paclitaxel and 5 μg/ml carboplatin on OS732 cells jointly, the survival rate was 15.8% which was significantly lower than with 10 μg/ml paclitaxel (45.8%) and 5 μg/ml carboplatin (47.7%) respectively (P<0.01). Moreover, changes of morphology and apoptotic rates indicated that the apoptosis-inducing effect of combined application was also much enhanced, as evident also regarding Fas expression.
  
  
  CONCLUSIONS
  Paclitaxel is conducive to thermochemotherapy of osteosarcoma cell lines, possibly accomplished by up-regulation of Fas expression with induction of apoptosis.
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China. huangtao@mail.cmu.edu.cn
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Gong
authors.forename: Xiu-Cheng
authors.initials: XC
authors.lastname: Li
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Zou
authors.forename: Guang-Jian
authors.initials: GJ
authors.lastname: Jiang
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Li
authors.forename: Dian-Peng
authors.initials: DP
authors.lastname: Feng
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D016190:Carboplatin / Q000008:administration & dosage; D003131:Combined Modality Therapy; D005334:Fever; D006801:Humans; D007124:Immunoenzyme Techniques; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D017239:Paclitaxel / Q000008:administration & dosage; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000378:metabolism
chemical_list: C491901:FAS protein, human; D019014:fas Receptor; D016190:Carboplatin; D017239:Paclitaxel