title: Development of on-line high performance liquid chromatography (HPLC)-biochemical detection methods as tools in the identification of bioactives.
abstract: Biochemical detection (BCD) methods are commonly used to screen plant extracts for specific biological activities in batch assays. Traditionally, bioactives in the most active extracts were identified through time-consuming bio-assay guided fractionation until single active compounds could be isolated. Not only are isolation procedures often tedious, but they could also lead to artifact formation. On-line coupling of BCD assays to high performance liquid chromatography (HPLC) is gaining ground as a high resolution screening technique to overcome problems associated with pre-isolation by measuring the effects of compounds post-column directly after separation. To date, several on-line HPLC-BCD assays, applied to whole plant extracts and mixtures, have been published. In this review the focus will fall on enzyme-based, receptor-based and antioxidant assays.
authors.affiliation: Post-Harvest and Wine Technology Division, Agricultural Research Council, Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa.
authors.forename: Christiaan J
authors.initials: CJ
authors.lastname: Malherbe
authors.affiliation: Post-Harvest and Wine Technology Division, Agricultural Research Council, Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa.
authors.forename: Dalene
authors.identifier: 0000-0002-3680-142X
authors.initials: D
authors.lastname: De Beer
authors.affiliation: Post-Harvest and Wine Technology Division, Agricultural Research Council, Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa.|Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa.
authors.forename: Elizabeth
authors.identifier: 0000-0002-9717-9769
authors.initials: E
authors.lastname: Joubert
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D055808:Drug Discovery; D004791:Enzyme Inhibitors / Q000032:analysis; D004867:Equipment Design; D006801:Humans; D008024:Ligands; D055442:Metabolome; D009862:Online Systems; D064209:Phytochemicals / Q000032:analysis*; D048789:Phytoestrogens / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry*; D011960:Receptors, Estrogen / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D008024:Ligands; D064209:Phytochemicals; D048789:Phytoestrogens; D010936:Plant Extracts; D011960:Receptors, Estrogen