title: Thermoresponsive poly(N-isopropylacrylamide)-based block copolymer coating for optimizing cell sheet fabrication.
abstract: Thermoresponsive surfaces are prepared via a spin-coating method with a block copolymer consisting of poly(N-isopropylacrylamide) (PIPAAm) and poly(butyl methacrylate) (PBMA) on polystyrene surfaces. The PBMA block suppresses the removal of deposited PIPAAm-based polymers from the surface. The polymer coating affects the temperature-dependent cellular behavior of the surfaces with respect to protein adsorption. By adjusting layer thicknesses, PBMA-b-PIPAAm-coated surfaces are optimized to regulate the adhesion/detachment of cells by temperature changes. Thus, thermoresponsive polymer-coated surfaces are able to harvest contiguous cell sheets with their basal extracellular matrix proteins.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University-WIns, 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nakayama
authors.forename: Naoko
authors.initials: N
authors.lastname: Yamada
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kumashiro
authors.forename: Hideko
authors.initials: H
authors.lastname: Kanazawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D000818:Animals; D002339:Carotid Arteries / Q000166:cytology* / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000378:metabolism; D006358:Hot Temperature; D011108:Polymers / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; D016326:Extracellular Matrix Proteins; D011108:Polymers; D011109:Polymethacrylic Acids; D011137:Polystyrenes; C052970:poly-N-isopropylacrylamide; C068872:polybutyl methacrylate