title: Sex differences in transcriptional expression of FABPs in zebrafish liver after chronic perfluorononanoic acid exposure.
abstract: Perfluorononanoic acid (PFNA), a nine carbon backbone of perfluorinated acids (PFAAs), has wide production applications and is found in environmental matrices as a contaminant. To understand the adverse effects of PFNA, adult male and female zebrafish were exposed to differing PFNA dosages (0, 0.01, 0.1, and 1.0 mg/L) for 180 days using a flow-through exposure system. Results showed body weight, body length, and hepatosomatic index (HSI) decreased in both sexes. The HPLC-MS/MS analysis found that PFNA concentrations were higher in male livers than in female livers with increasing significance in a dose-dependent manner. Total cholesterol levels increased in the livers of both sexes, whereas triglyceride (TG) levels increased in males and decreased in females. With the exception of FABP1b, the transcriptional expression levels of fatty acid binding proteins (FABPs) were up-regulated in males and down-regulated in females. A similar trend between sexes occurred for peroxisome proliferator-activated receptors (PPARs) and Ccaat-enhancer-binding proteins (C/EBPs), which may be the upstream regulatory elements of FABPs. The results indicated that PFNA exposure caused opposite adverse effects on liver TG levels between the sexes in zebrafish possibly due to the opposite expression of FABPs and its upstream genes.
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yating
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhang
authors.forename: Jianshe
authors.initials: J
authors.lastname: Wang
authors.forename: Ruina
authors.initials: R
authors.lastname: Cui
authors.forename: Jiayin
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D004785:Environmental Pollutants / Q000008:administration & dosage*; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D005260:Female; D005466:Fluorocarbons / Q000008:administration & dosage*; D015870:Gene Expression / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism; D012737:Sex Factors; D014280:Triglycerides / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D004785:Environmental Pollutants; D050556:Fatty Acid-Binding Proteins; D005466:Fluorocarbons; D047492:Peroxisome Proliferator-Activated Receptors; D014280:Triglycerides; C018959:hexadecafluoro-nonanoic acid; D002784:Cholesterol