title: [Raman spectroscopic study on the influence of ultraviolet-A radiation on collagen I].
abstract: Raman spectroscopy was used to study the influence of ultraviolet-A(UV-A) radiation on collagen I. The Raman spectra of collagen I and that after 90 min UV-A radiation were reported. The results proved that irradiation with 90 min UV-A caused the change in the structures of collagen I. Intramolecular hydrogen bonds were broken, and the hydrogen bonding system was changed. The intensity of helix was decreased, while the intensity of the disordered conformation in proteins such as random coil was increased. Otherwise, the UV-A radiation influenced the hydroxylation of proline and the content of hydroxyproline was reduced. The changes caused by UV-A radiation could damage the triple helical structure of collagen I. It would lead to a series of changes, such as the destruction of collagen fibers during the photoaging of skin.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Laboratory of Photonic Chinese Medicine, College of Biophotonics, South China Normal University, Guangzhou 510631, China. marongtian@126.com
authors.forename: Rong-tian
authors.initials: RT
authors.lastname: Ma
authors.forename: Zhou-yi
authors.initials: ZY
authors.lastname: Guo
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Liu
authors.forename: Hui-qing
authors.initials: HQ
authors.lastname: Zhong
authors.forename: Zheng-fei
authors.initials: ZF
authors.lastname: Zhuang
authors.forename: Juan
authors.initials: J
authors.lastname: Zhai
mesh_terms: D003094:Collagen; D006860:Hydrogen Bonding; D006900:Hydroxylation; D011392:Proline; D017433:Protein Structure, Secondary; D011506:Proteins; D013059:Spectrum Analysis, Raman; D014466:Ultraviolet Rays
chemical_list: D011506:Proteins; D003094:Collagen; D011392:Proline