title: Methylation of the DFNA5 gene is frequently detected in colorectal cancer.
abstract: BACKGROUND
  Recently, the human deafness, autosomal dominant 5 gene, DFNA5, has frequently been detected in cancer tissues. The methylation status of the DFNA5 gene in colorectal cancer was examined and was compared to the clinocopathological findings.
  
  
  METHODS
  Eighty-five tumor samples and corresponding normal tissues were obtained from patients with colorectal cancer who underwent surgery at our hospital. The methylation status of the DFNA5 gene in these samples was examined by quantitative methylation-specific PCR (qMSP). Subsequently, the clinicopathological findings were correlated with the methylation status of the DFNA5 gene.
  
  
  RESULTS
  DFNA5 gene methylation was found in 29 (34%) out of the 85 colorectal carcinomas, suggesting that it was frequently observed in colorectal cancer. A significant correlation with methylation was observed for lymphatic vessel invasion and TNM stage (p=0.0268 and p=0.0189, respectively).
  
  
  CONCLUSIONS
  DFNA5 might act as a tumor suppressor gene and DFNA5 gene methylation might play an important role in the development of colorectal cancer. Our data implicate DFNA5 gene methylation as a novel molecular biomarker in colorectal cancer.
authors.affiliation: Gastroenterological Surgery, Showa University Fujigaoka Hospital, Aoba-ku, Yokohama, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yokomizo
authors.forename: Yoshikuni
authors.initials: Y
authors.lastname: Harada
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kijima
authors.forename: Kazuki
authors.initials: K
authors.lastname: Shinmura
authors.forename: Makiko
authors.initials: M
authors.lastname: Sakata
authors.forename: Kazuma
authors.initials: K
authors.lastname: Sakuraba
authors.forename: Youhei
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shirahata
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Goto
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mizukami
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Saito
authors.forename: Gaku
authors.initials: G
authors.lastname: Kigawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nemoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Hibi
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D019175:DNA Methylation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000235:genetics*; D000094524:Gasdermins
chemical_list: D011960:Receptors, Estrogen; C111432:GSDME protein, human; D000094524:Gasdermins