title: Recording and sorting live human sperm undergoing acrosome reaction.
abstract: OBJECTIVE
  To develop and evaluate a method to detect acrosome reaction (AR) in live human sperm.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Basic research laboratory.
  
  
  METHODS
  Human semen samples with normal parameters obtained from healthy donors.
  
  
  METHODS
  Acrosome reaction assays.
  
  
  METHODS
  Fluorescence assessment of AR.
  
  
  RESULTS
  Evaluating acrosomal exocytosis in live human sperm is challenging. In this study, we report that in reacting sperm, Pisum sativum agglutinin conjugated to fluorescein isothiocyanate rapidly permeates into the acrosome when fusion pores open and stabilizes the acrosomal matrix, preventing the dispersal of the granule contents.
  
  
  CONCLUSIONS
  Fluorescent Pisum sativum agglutinin can be used to visualize AR in real time, to determine the percentage of sperm undergoing exocytosis upon stimulation, and to separate the population of reacting sperm by flow cytometry.
authors.affiliation: Instituto de Histología y Embriología Dr Mario H Burgos, Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Cuyo, School of Medicine, National University of Cuyo, Mendoza, Argentina.
authors.forename: Felipe Carlos Martín
authors.initials: FC
authors.lastname: Zoppino
authors.forename: Narciso D
authors.initials: ND
authors.lastname: Halón
authors.forename: Matías A
authors.initials: MA
authors.lastname: Bustos
authors.forename: Martín A
authors.initials: MA
authors.lastname: Pavarotti
authors.forename: Luis S
authors.initials: LS
authors.lastname: Mayorga
mesh_terms: D020101:Acrosome Reaction / Q000502:physiology*; D005089:Exocytosis / Q000502:physiology; D005434:Flow Cytometry / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D008297:Male; D018532:Pisum sativum; D037121:Plant Lectins; D011446:Prospective Studies; D055101:Semen Analysis / Q000379:methods*; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*; D014743:Videotape Recording / Q000379:methods
chemical_list: D037121:Plant Lectins; D016650:Fluorescein-5-isothiocyanate