title: Nitrogen source and growth stage of  Candida albicans  influence expression level of vacuolar aspartic protease Apr1p and carboxypeptidase Cpy1p.
abstract: Vacuoles play an important role in the physiology of pathogenic  Candida  spp. However, information on  Candida albicans  vacuolar enzymes, their properties, and regulation is scarce. Expression of the genes APR1 and CPY1 encoding vacuolar aspartic protease and serine carboxypeptidase, respectively, was analyzed using a clinical isolate of C. albicans. The transcription of both APR1 and CPY1 was upregulated in midexponential phase, together with increasing size of the vacuoles, when C. albicans was cultivated in yeast extract-peptone-dextrose agar at 30 °C. However, simultaneous upregulation of protein synthesis occurred only for Cpy1p. Analysis of APR1 and CPY1 expression under nitrogen-limited conditions revealed that the genes were regulated on both the transcriptional and translational levels and detectable amounts of Apr1p were synthesized only when C. albicans was grown in nitrogen-limited media.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Gilead & IOCB Research Centre, Flemingovo namesti 2, 166 10 Prague 6, Czech Republic.
authors.forename: Václava
authors.initials: V
authors.lastname: Bauerová
authors.forename: Iva
authors.initials: I
authors.lastname: Pichová
authors.forename: Olga
authors.initials: O
authors.lastname: Hrušková-Heidingsfeldová
mesh_terms: D057055:Aspartic Acid Proteases / Q000235:genetics / Q000378:metabolism*; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D002268:Carboxypeptidases / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D009584:Nitrogen / Q000378:metabolism*; D015854:Up-Regulation; D014617:Vacuoles / Q000201:enzymology*
chemical_list: D003470:Culture Media; D057055:Aspartic Acid Proteases; D002268:Carboxypeptidases; C022633:serine carboxypeptidase; D009584:Nitrogen