title: Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves.
abstract: Using real-time PCR, we tested 15 wolf (Canis lupus) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 10(4) 50% tissue culture infective dose. Virus was detected in two winter specimens but none of the summer specimens. We suggest applying the technique more broadly especially with winter feces.
authors.affiliation: US Geological Survey, Northern Prairie Wildlife Research Center, 8711 37th St SE, Jamestown, North Dakota 58401-7317, USA. david_mech@usgs.gov
authors.forename: L David
authors.initials: LD
authors.lastname: Mech
authors.forename: Emily S
authors.initials: ES
authors.lastname: Almberg
authors.forename: Douglas
authors.initials: D
authors.lastname: Smith
authors.forename: Sagar
authors.initials: S
authors.lastname: Goyal
authors.forename: Randall S
authors.initials: RS
authors.lastname: Singer
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005243:Feces / Q000821:virology*; D017993:Parvovirus, Canine / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000592:standards / Q000662:veterinary*; D015203:Reproducibility of Results; D012621:Seasons; D012680:Sensitivity and Specificity; D019596:Wolves / Q000821:virology
chemical_list: D004279:DNA, Viral