title: Expression of MMP-2, TIMP-2, TGF-β1, and decorin in Dupuytren's contracture.
abstract: To investigate the mechanisms underlying matrix deposition in Dupuytren's disease, the expression of gelatinase A (MMP-2), the tissue inhibitor of metalloproteinase-2 (TIMP-2), transforming growth factor beta 1 (TGF-β1), decorin (DCN), and periostin was studied. The level of relative MMP-2 activation was investigated using zymography. The mRNA expression of MMP-2, TIMP-2, TGF-β1, and DCN was detected using reverse transcription polymerase chain reaction (RT-PCR), while the presence of protein was detected using immunohistochemical (IHC) and Western blot techniques. The level of MMP-2 activation was significantly elevated in tissues with Dupuytren's contracture. RT-PCR demonstrated significantly higher expression of MMP-2, TIMP-2, TGF-β1, and DCN mRNA in the pathological tissues; and the IHC and immunoblotting studies revealed elevated expression of TGF-β1, DCN, and periostin. The balance between MMP-2 and TIMP-2 was disrupted in patients with Dupuytren's disease. TGF-β1, DCN, and periostin are involved in extracellular matrix (ECM) homeostasis in Dupuytren's contracture.
authors.affiliation: Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland. katarzyna.ratajczak-wielgomas@am.wroc.pl
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Ratajczak-Wielgomas
authors.forename: Jerzy
authors.initials: J
authors.lastname: Gosk
authors.forename: Jerzy
authors.initials: J
authors.lastname: Rabczyński
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Augoff
authors.forename: Marzenna
authors.initials: M
authors.lastname: Podhorska-Okołów
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
authors.forename: Roman
authors.initials: R
authors.lastname: Rutowski
mesh_terms: D000368:Aged; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D058575:Decorin / Q000096:biosynthesis*; D004387:Dupuytren Contracture / Q000378:metabolism* / Q000473:pathology; D004789:Enzyme Activation; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis*; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*
chemical_list: D015815:Cell Adhesion Molecules; C546878:DCN protein, human; D058575:Decorin; C512835:POSTN protein, human; D012333:RNA, Messenger; C508960:TGFB1 protein, human; C560823:TIMP2 protein, human; D053773:Transforming Growth Factor beta1; D019716:Tissue Inhibitor of Metalloproteinase-2; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2