title: Polyomavirus-induced pilomatricomas in mice: from viral inoculation to tumour development.
abstract: Polyomavirus has been used extensively to study tumour induction in mice. Although most neoplasms are well characterized, those arising from hair follicles have been referred to by different names during the last four decades. The purpose of this research was to contribute to a more accurate histological characterization of these tumours as well as to study the viral progression from the onset of infection to the development of neoplasms. Polyomavirus A2 was inoculated into newborn C3H/BiDa mice, and at different time-points (from 5 to 70 days post-inoculation) the mice were sacrificed and studied using histological, immunocytochemical, ultrastructural and virological methods. The fully developed hair follicle tumours consisted of a proliferation of matrix cells that evolved into 'shadow' cells with empty nuclei and finally into amorphous keratin; the tumours were therefore diagnosed as pilomatricomas. Viral VP-1 was observed only in fully differentiated cells and not in proliferating-cell-nuclear-antigen (PCNA)-positive cells in the same tumour. In conclusion, Polyomavirus first replicated in the skin, and then disseminated through the blood and reached the outer sheath of the hair follicles and finally infected matrix cells, leading to the development of pilomatricomas from which infectious virus was isolated.
authors.affiliation: Departments of Microbiology, University of Buenos Aires, School of Medicine, Argentina.
authors.forename: Silvina
authors.initials: S
authors.lastname: Símula
authors.forename: Paola Villán
authors.initials: PV
authors.lastname: Ozuna
authors.forename: Javier
authors.initials: J
authors.lastname: Otero
authors.forename: José
authors.initials: J
authors.lastname: Casas
authors.forename: Norberto
authors.initials: N
authors.lastname: Sanjuan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006201:Hair Diseases / Q000473:pathology / Q000821:virology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells; D018296:Pilomatrixoma / Q000473:pathology / Q000648:ultrastructure / Q000821:virology*; D011120:Polyomavirus / Q000502:physiology*; D027601:Polyomavirus Infections / Q000473:pathology* / Q000821:virology; D012878:Skin Neoplasms / Q000473:pathology / Q000648:ultrastructure / Q000821:virology*; D014779:Virus Replication