title: Mandibular reconstruction in the rabbit using beta-tricalcium phosphate (β-TCP) scaffolding and recombinant bone morphogenetic protein 7 (rhBMP-7) - histological, radiographic and mechanical evaluations.
abstract: This investigation assesses the histological, radiographic and mechanical properties of regenerated bone in a unilateral critical-size osteoperiosteal mandibular continuity defect in the rabbit model, following the application of beta-tricalcium phosphate (β-TCP) scaffolding and recombinant human bone morphogenetic protein 7 (rhBMP-7). The study was carried out on nine cases; in six cases the critical-size defect was filled with rhBMP-7 in the β-TCP scaffolding, and in three cases the β-TCP was used alone. The cases were sacrificed 3 months post-operatively. Histologically the overall mean of the percentage of regenerated bone volume in the cases that received rhBMP-7 was 29.41% ± 6.25%, which was considerably greater than the 6.35% ± 3.08% in the cases treated with β-TCP alone. Mechanical testing of the cases treated with rhBMP-7 gave failure moments (55 mNm-2.040 Nm) that were consistently greater than those treated with β-TCP alone (0 mNm-48 mNm). In some cases the mechanical properties of the regenerated bone were comparable to those of untreated bone. RhBMP-7 in prefabricated β-TCP scaffolding appeared, radiographically and histologically, to be an effective method for bone regeneration in mandibular critical-size defects in the rabbit model. This points towards possible future clinical applications.
authors.affiliation: Biotechnology & Craniofacial Sciences Research Group, Glasgow Dental Hospital, UK. kurt.naudi@dental.gla.ac.uk
authors.forename: Kurt
authors.initials: K
authors.lastname: Busuttil Naudi
authors.forename: Ashraf
authors.initials: A
authors.lastname: Ayoub
authors.forename: Jeremy
authors.initials: J
authors.lastname: McMahon
authors.forename: Lucy
authors.initials: L
authors.lastname: Di Silvio
authors.forename: David
authors.initials: D
authors.lastname: Lappin
authors.forename: Keith D
authors.initials: KD
authors.lastname: Hunter
authors.forename: Joseph
authors.initials: J
authors.lastname: Barbenel
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001696:Biomechanical Phenomena; D015519:Bone Density / Q000187:drug effects / Q000502:physiology; D055419:Bone Morphogenetic Protein 7 / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology; D016723:Bone Remodeling / Q000187:drug effects / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry; D054893:Cone-Beam Computed Tomography / Q000379:methods; D004195:Disease Models, Animal; D006801:Humans; D008334:Mandible / Q000473:pathology / Q000601:surgery; D008336:Mandibular Diseases / Q000473:pathology / Q000601:surgery; D008337:Mandibular Fractures / Q000503:physiopathology; D063175:Mandibular Reconstruction / Q000295:instrumentation / Q000379:methods*; D011817:Rabbits; D019651:Plastic Surgery Procedures / Q000295:instrumentation / Q000379:methods*; D013314:Stress, Mechanical; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: C525819:BMP7 protein, human; D001672:Biocompatible Materials; D055419:Bone Morphogenetic Protein 7; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate