title: Fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enhancing enzymatic saccharification and hydrogen production.
abstract: The feasibility of fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enzymatic saccharification and H(2) production was investigated in this study. Firstly, cornstalk was pretreated with P. chrysosporium at 29 °C under static condition for 15 d, lignin reduction was up to 34.3% with holocellulose loss less than 10%. Microscopic structure observation combined FTIR analysis further demonstrated that the lignin and crystallinity were decreased. Subsequently, the fungal-pretreated cornstalk was subjected to enzymatic hydrolysis by the crude cellulase from Trichoderma viride to produce fermentable sugars which were then fermented to bio-H(2) using Thermoanaerobacterium thermosaccharolyticum W16. The maximum enzymatic saccharification was found to be 47.3% which was 20.3% higher than the control without pretreatment. Upon fermentation of enzymatic hydrolysate, the yield of H(2) was calculated to be 80.3 ml/g-pretreated cornstalk. The present results suggested the potential of using hydrogen-producing bacteria for high-yield conversion of cornstalk into bio-H(2) integrate with biological pretreatment and enzymatic saccharification.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Cao
authors.forename: Ai-Jie
authors.initials: AJ
authors.lastname: Wang
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Ren
authors.forename: De
authors.initials: D
authors.lastname: Dong
authors.forename: Zi-Nan
authors.initials: ZN
authors.lastname: Liu
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Guan
authors.forename: Cheng-Jiao
authors.initials: CJ
authors.lastname: Xu
authors.forename: Nan-Qi
authors.initials: NQ
authors.lastname: Ren
mesh_terms: D050260:Carbohydrate Metabolism / Q000502:physiology*; D006859:Hydrogen / Q000302:isolation & purification* / Q000378:metabolism*; D007220:Industrial Waste / Q000517:prevention & control*; D020075:Phanerochaete / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000382:microbiology*; D003313:Zea mays / Q000382:microbiology*
chemical_list: D007220:Industrial Waste; D006859:Hydrogen