title: Screening peptides binding specifically to colorectal cancer cells from a phage random peptide library.
abstract: The aim of this study was to screen for polypeptides binding specifically to LoVo human colorectal cancer cells using a phage-displayed peptide library as a targeting vector for colorectal cancer therapy. Human normal colorectal mucous epithelial cells were applied as absorber cells for subtraction biopanning with a c7c phage display peptide library. Positive phage clones were identified by enzyme-linked immunosorbent assay and immunofluorescence detection; amino acid sequences were deduced by DNA sequencing. After 3 rounds of screening, 5 of 20 phage clones screened positive, showing specific binding to LoVo cells and a conserved RPM motif. Specific peptides against colorectal cancer cells could be obtained from a phage display peptide library and may be used as potential vectors for targeting therapy for colorectal cancer.
authors.affiliation: Department of Gastroenterology Surgery, Guangdong General Hospital, Guangzhou, China.
authors.forename: Jun-Jiang
authors.initials: JJ
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Zheng
authors.forename: Kang-Xiong
authors.initials: KX
authors.lastname: Liao
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.forename: Cheng-Tang
authors.initials: CT
authors.lastname: Wu
authors.forename: Guan-Fu
authors.initials: GF
authors.lastname: Cai
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Yao
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D010446:Peptide Fragments / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019151:Peptide Library; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured
chemical_list: D010446:Peptide Fragments; D019151:Peptide Library