title: [Role of anti c-mpl antibody in systemic lupus erythematosus with thrombocytopenia].
abstract: OBJECTIVE
  To determine whether anti-thrompoietin receptor (TPO-R, c-mpl) antibody contributes to thrombocytopenia in systemic lupus  erytematosus (SLE) and explore the pathogenic role of this antibody.
  
  
  METHODS
  Sera from 24 SLE patients with thrombocytopenia, 27 SLE patients  having normal platelet counts with a history of thrombocytopenia, 18 SLE patients with neither thrombocytopenia nor post thrombocytopenia and 18 healthy  controls were collected. Anti c-mpl antibodies were detected by an indirected ELISA assay. The serum TPO levels were measured by an ELISA assay.  Clinical findings, autoantibody profiles, and SLEDAI were evaluated.
  
  
  RESULTS
  Serum anti c-mpl antibodies were detected in 18.8% of the SLE patientis.  The frequency of this antibody in SLE with thrombocytopenia, SLE with a history of thrombocytopenia and SLE without thrombocytopenia were of no  difference (P=0.600). In the patients with anti c-mpl antibodies, their platelet counts were decreased(P=0.025) and serum TPO levels elevated(P=0.038)  than those in the patients without, while there were no differences between the two groups in C3, C4, ESR, CRP level, the frequency of ANA, dsDNA, ANCA  and SLEDAI.
  
  
  CONCLUSIONS
  Anti c-mpl antibody contributes to SLE-associated thrombocytopenia by functionally blocking an interaction between  thrombopoietin and c-mpl, which might inhibit TPO-dependent megakaryocyte proliferation and differentiation.
authors.affiliation: Department of Rheumatology and Immunology, Beijing Hospital, Beijing 100730, China.
authors.forename: Tuo
authors.initials: T
authors.lastname: Yang
authors.forename: Ci Bo
authors.initials: CB
authors.lastname: Huang
authors.forename: Bei
authors.initials: B
authors.lastname: Lai
authors.forename: Li Ke
authors.initials: LK
authors.lastname: Zhao
authors.forename: Ying Juan
authors.initials: YJ
authors.lastname: Chen
authors.forename: Yue Tao
authors.initials: YT
authors.lastname: Zhao
authors.forename: Chun Mei
authors.initials: CM
authors.lastname: Zhang
authors.forename: Xiao Feng
authors.initials: XF
authors.lastname: Zeng
mesh_terms: D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000276:immunology* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D053628:Receptors, Thrombopoietin / Q000276:immunology*; D013921:Thrombocytopenia / Q000150:complications* / Q000503:physiopathology; D013926:Thrombopoietin / Q000097:blood; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D053628:Receptors, Thrombopoietin; C082218:MPL protein, human; D013926:Thrombopoietin