title: Fine visualization of filamentous structures in the bacterial cytoplasm.
abstract: A new method was established for fine visualization of bacterial subcelluar filamentous structures by freezing the bacterial cells to displace cytoplasmic matrix granules to the periphery. This method was successfully applied in immunoelectron microscopy and electron microscopic tomography, and should be applicable for further studies of bacterial architecture and nanotransportation.
authors.affiliation: Department of Microbiology and Infection Control, Osaka Medical College, 2-7, Daigaku-machi, Takatsuki-shi, Osaka 569-8686 Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakano
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Aoki
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Fujioka
authors.forename: Eiko
authors.initials: E
authors.lastname: Nakazawa
authors.forename: Kouichi
authors.initials: K
authors.lastname: Sano
mesh_terms: D003593:Cytoplasm / Q000648:ultrastructure*; D005615:Freezing; D016480:Helicobacter pylori / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D016253:Microscopy, Immunoelectron / Q000379:methods*