title: [Optimization on decoloration conditions of anthraquinone dyes by laccase from Amillariella mellea].
abstract: Laccase extracted from the Amillariella mellea fermentation catalytic decolored on two common anthraquinone dyes: Reactive Brilliant Blue KN-R and Reactive Brilliant Blue X-BR which is broadly used in the printing and dyeing industry and obtained the optimal catalytic decolorizing conditions. The results showed that optimum temperature of Reactive Brilliant Blue KN-R decolorization was 30 degrees C, the optimum dye concentrations was 80 mg x L(-1), the optimum enzyme dosage was 0.25 U x mL(-1), and the optimum pH was 5. Under this optimal conditions, the maximum decolorization rate of Reactive Brilliant Blue KN-R was over 90%. The optimum temperature Reactive Brilliant Blue X-BR decolorization was 30 degrees C, the optimum dye concentrations was 50 mg x L(-1), the optimum enzyme dosage was 0.5 U x mL(-1), and the optimum was pH 4. Under the optimal conditions, the maximum decolorization rate of Reactive Brilliant Blue X-BR was over 70%. The decolorization on the two common industrial dyes by crude enzyme from Amillariella mellea fermentation obtained a good results. The results indicated that the decoloration on anthraquinone dyes by laccase from Amillariella mellea has a potential value in the printing and dyeing industry.
authors.affiliation: Institute of Bioengineering, College of Life Science, Henan University, Kaifeng 475004, China. zhuxf73@163.com
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Zhu
authors.forename: Ren-Bing
authors.initials: RB
authors.lastname: Qin
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Yu
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Fan
mesh_terms: D000880:Anthraquinones / Q000302:isolation & purification* / Q000378:metabolism; D001557:Benzenesulfonates / Q000302:isolation & purification / Q000378:metabolism; D004396:Coloring Agents / Q000302:isolation & purification* / Q000378:metabolism; D005658:Fungi / Q000145:classification / Q000201:enzymology*; D042845:Laccase / Q000378:metabolism*; D013783:Textile Industry; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry
chemical_list: D000880:Anthraquinones; D001557:Benzenesulfonates; D004396:Coloring Agents; D042845:Laccase; D062065:Wastewater; C006796:brilliant blue