title: Genetic diversity and naturally polymorphisms in HIV type 1 integrase isolates from Maputo, Mozambique: implications for integrase inhibitors.
abstract: HIV proviral DNA integration into the host chromosome is carried out by integrase becoming an important target antiretroviral therapy. Raltegravir was the first integrase inhibitor approved for use in HIV therapy and elvitegravir is in the late phase of clinical development; both show good results in monotherapy studies and may be used worldwide for rescue therapy. In this work we analyzed 57 integrase sequences obtained from samples from drug-naive and first line regime-failing patients from Maputo, Mozambique, to evaluate the presence of natural polymorphisms and resistance mutations associated with raltegravir and elvitegravir. No major mutations conferring resistance to integrase inhibitors were found and polymorphic accessory mutations were solely observed in low frequency among subtype C sequences-L74M (3.4%), T97A (1.8%), and E157Q (1.8%)-suggesting that this new antiretroviral drug class will be effective in Mozambique providing a good perspective to the introduction of this class of drugs in that country.
authors.affiliation: Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Michelli F
authors.initials: MF
authors.lastname: Oliveira
authors.forename: Dulce B
authors.initials: DB
authors.lastname: Ramalho
authors.forename: Celina M
authors.initials: CM
authors.lastname: Abreu
authors.forename: Adolfo
authors.initials: A
authors.lastname: Vubil
authors.forename: Nédio
authors.initials: N
authors.lastname: Mabunda
authors.forename: Nalia
authors.initials: N
authors.lastname: Ismael
authors.forename: Cidia
authors.initials: C
authors.lastname: Francisco
authors.forename: Ilesh V
authors.initials: IV
authors.lastname: Jani
authors.forename: Amilcar
authors.initials: A
authors.lastname: Tanuri
mesh_terms: D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D024882:Drug Resistance, Viral; D014644:Genetic Variation; D015658:HIV Infections / Q000821:virology*; D019427:HIV Integrase / Q000235:genetics*; D019428:HIV Integrase Inhibitors / Q000627:therapeutic use*; D006801:Humans; D008969:Molecular Sequence Data; D009073:Mozambique; D020125:Mutation, Missense; D011533:Proviruses / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral; D019428:HIV Integrase Inhibitors; D019427:HIV Integrase