title: Ethylene response pathway is essential for ARABIDOPSIS A-FIFTEEN function in floral induction and leaf senescence.
abstract: ARABIDOPSIS A-FIFTEEN (AAF) encodes a plastid protein and was originally identified as a SENESCENCE-ASSOCIATED GENE. Previously, we found that overexpression of AAF (AAF-OX) in Arabidopsis led to accumulated reactive oxygen species and promoted leaf senescence induced by oxidative stress, which was suppressed by a null mutant, ein2-5, in ethylene response pathway. Whether AAF function is involved in ethylene biosynthesis and/or the response pathway remained unknown. Here we show that neither overexpression (AAF-OX) nor a null mutant (aaf-KO) of AAF generates a higher level of ethylene than the wild type and display a typical triple-response phenotype in etiolated seedlings treated with 1-aminocyclopropane-1-carboxylic acid (ACC). Nevertheless, ein2-5 suppresses the phenotypes of early flowering and age-dependent leaf senescence in AAF-OX plants. We reveal that a functional ethylene response is essential for AAF function in leaf senescence and floral induction, but AAF is unlikely a regulatory component integral to the ethylene pathway.
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Guan-Hong
authors.initials: GH
authors.lastname: Chen
authors.forename: Yuan-Li
authors.initials: YL
authors.lastname: Chan
authors.forename: Chia-Ping
authors.initials: CP
authors.lastname: Liu
authors.forename: Long-Chi
authors.initials: LC
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060365:Chloroplast Proteins / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism* / Q000494:pharmacology; D035264:Flowers / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects; D018525:Germination / Q000187:drug effects / Q000528:radiation effects; D018546:Hypocotyl / Q000033:anatomy & histology / Q000187:drug effects / Q000528:radiation effects; D008027:Light; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000528:radiation effects; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects
chemical_list: D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; C119963:EIN2 protein, Arabidopsis; D005030:Ethylenes; D010937:Plant Growth Regulators; D011956:Receptors, Cell Surface; C036216:ethylene