title: NEIL1 mRNA splicing variants are expressed in normal mouse organs.
abstract: Oxidized pyrimidines are mainly repaired by base excision repair, which is initiated by damage-specific DNA glycosylases. NEIL1, the mammalian homolog of Escherichia coli endonuclease VIII and a major DNA glycosylase, initiates repair of oxidized pyrimidines. Here, we investigated the expression of two putative variant mouse NEIL1 (mNEIL1) mRNAs--variant 1 ("Neil1 protein" mRNA; BC043297 in the NCBI database) and variant 2 ("unnamed protein" mRNA; AK040802 in the NCBI database)--in normal mouse organs. Reverse transcription-PCR showed that both mRNAs were expressed in total RNA samples from 9 organs. Immunoblot analysis of a nuclear extract from normal mouse liver revealed three bands corresponding to full-length mNEIL1 protein and the two predicted variant proteins. However, neither variant protein, which included an N-terminal enzymatic activity domain deduced from the mRNA variants, were enzymatically active under multiple reaction conditions when expressed as his-tagged recombinant proteins. Nevertheless, recombinant variant 1 protein influenced mNEIL1 activity, while recombinant variant 2 protein had no influence. These results suggest that mNEIL1 mRNA variants are expressed in a variety of organs in normal mice and that variant 1 protein may regulate mNEIL1 activity.
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka 598-8531, Japan. yamamoto@vet.osakafu-u.ac.jp
authors.forename: Ryohei
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Mizuki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kusaka
authors.forename: Hideaki
authors.initials: H
authors.lastname: Masatsugu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Matsuyama
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tajima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ide
authors.forename: Kihei
authors.initials: K
authors.lastname: Kubo
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D045647:DNA Glycosylases / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D009928:Organ Specificity / Q000235:genetics*; D012326:RNA Splicing / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012016:Reference Values; D014018:Tissue Distribution; D014781:Viscera / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D045647:DNA Glycosylases; C481347:Neil1 protein, mouse