title: In vitro identification of DNA-binding motif for the new zinc finger protein AtYY1.
abstract: The functional characterization of novel transcription factors identified by systematic analysis remains a major challenge due to insufficient data to interpret their specific roles in signaling networks. Here we present a DNA-binding sequence discovery method to in vitro identify a G-rich, 11-bp DNA-binding motif of a novel potential transcription factor AtYY1, a zinc finger protein in Arabidopsis, by using polymerase chain reaction-assisted in vitro selection and surface plasmon resonance analysis. Further mutational analysis of the conserved G bases of the potential motif confirmed that AtYY1 specifically bound to these conserved G sites. Additionally, genome-wide target gene analysis revealed that AtYY1 was involved in diverse cellular pathways, including glucose metabolism, photosynthesis, phototropism, and stress response.
authors.affiliation: Laboratory of Molecular Biology and Protein Science Laboratory of the Ministry of Education, School of Life Sciences,  Tsinghua University, Beijing 100084,  China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Wu
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Cheng
authors.forename: Tian
authors.initials: T
authors.lastname: Li
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.forename: Jin-Yuan
authors.initials: JY
authors.lastname: Liu
mesh_terms: D017360:Arabidopsis / Q000737:chemistry; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D020349:Surface Plasmon Resonance; D050986:YY1 Transcription Factor / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D050986:YY1 Transcription Factor