title: Adipose tissue engineering using adipose-derived stem cells enclosed within an injectable carboxymethylcellulose-based hydrogel.
abstract: In situ gelation of an aqueous solution of carboxymethylcellulose derivative bearing phenolic hydroxyl groups (CMC-Ph) that contained suspended adipose-derived stem cells (ASCs) was studied in vitro and in vivo for evaluating feasibility in adipose tissue-engineering strategies. The rat ASCs that were enclosed in the CMC-Ph gels through a horseradish peroxidase-catalysed reaction showed 92.8% viability, good proliferation and adipogenic differentiation in vitro. Ten weeks after the subcutaneous injection of ASCs-suspending CMC-Ph for in situ gelation, clearly visible new vascularized adipose tissue formed at the injection site. The number of blood vessels and the area occupied by adipose tissues were five and eight times larger, respectively, than those found in the implanted acellular gel. The adipogenesis and neovascularization were further enhanced by incorporation of fibroblast growth factor into the CMC-Ph gel containing ASCs.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ogushi
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.forename: Koei
authors.initials: K
authors.lastname: Kawakami
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D007267:Injections; D007279:Injections, Subcutaneous; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010636:Phenols / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D010636:Phenols; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002266:Carboxymethylcellulose Sodium