title: [Diagnostic significance of autoantibodies in patients with primary biliary cirrhosis].
abstract: OBJECTIVE
  To investigate the significance of autoantibodies in patients with primary biliary cirrhosis (PBC).
  
  
  METHODS
  A anti-mitochondrial  antibodies-M2(AMA-M2), anti-BCOADC-E2 PDC-E2 OGDC-E2 antibodies (anti-3E/BPO), anti-SP100 antibodies (anti-SP100), anti-promyelocytic leukemia (anti-PML), anti-gp210 antibodies (anti-gp210),and anti-Ro-52 were detected respectively in 330 suspected PBC cases by  Western blotting.
  
  
  RESULTS
  (1) The sensitivity/specificity rates of AMA-M2,anti-3E/BPO,anti-SP100,anti-PML,anti-gp210,and anti-Ro-52 were  85.3%/84.8%, 79.4%/93.2 %, 35.3%/98.0%, 41.2%/96.3%, 44.1%/96.6%,61.8%/68.6% respectively; AMA-M2 were combined with the other antibodies. The  specificity rates in the series tests were 94.9%, 99.3%, 99.3 %, 98.3%, and 92.2%, while the sensitivity rates in the parallel tests were 91.2%, 94.1%, 94.1%,  94.1%, and 1.2%,respectively .(2)There were 5 cases of AMA-M2 negative in patients with PBC,including 60% (3/5) cases of anti-gp210, anti-SP100 and  anti-PML positive respectively.
  
  
  CONCLUSIONS
  (1) AMA-M2 were more sensitive than other antibodies, while the specificity rates of anti-3E/BPO , anti-SP100, anti-PML, and anti-gp210 were higher than that of AMA-M2; Parallel tests helps to exclude the suspected PBC cases and series tests could be  useful in clinics to confirm the PBC cases. (2) anti-gp210, anti-SP100, anti-PML antibodies appear to be more common in AMA-M2 negative PBC  patients than in those who are AMA-M2 positive,and their presence in AMA-M2 negative PBC patients contributes to the PBC diagnosis.
authors.affiliation: Department of Rheumatology and Immunology, Xiangya Second Hospital, Central South University, Changsha 410011, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xiao
authors.forename: Jin-wei
authors.initials: JW
authors.lastname: Chen
authors.forename: Xi
authors.initials: X
authors.lastname: Xie
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Fen
authors.initials: F
authors.lastname: Li
mesh_terms: D000328:Adult; D034961:Antigens, Nuclear / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology; D005260:Female; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000175:diagnosis* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000276:immunology*; D028861:Nuclear Pore Complex Proteins / Q000276:immunology
chemical_list: D034961:Antigens, Nuclear; D001323:Autoantibodies; D001324:Autoantigens; C063865:NUP210 protein, human; D028861:Nuclear Pore Complex Proteins; C066642:SP100 protein, human