title: [Effects of ecdysterone on the expression of NF-kappaB p65 in H2O2 induced oxidative damage of human lens epithelial cells].
abstract: OBJECTIVE
  To study the effects of ecdysterone (ECR) on the expression of nuclear factor (NF)-kappaB in H2O2 induced oxidative damage of human lens epithelial cells (HLECs).
  
  
  METHODS
  The cultured HLECs were divided into 5 groups, i.e., the control group, the H2O2 group, the beta-estradiol (E2) group, the ECR group, and the pyrrolidine dithiocarbamate group (PDTC) group. The expression rate of NF-kappaB p65 in the HLECs were detected by flow cytometer (FCM).
  
  
  RESULTS
  The expression of NF-kappaB p65 occurred in normal HLECs (9. 53%). The expression rate of NF-kappaB p65 in the H2O2 group obviously increased (39.87%, P < 0.01). The expression rate of NF-kappaB p65 in the PDTC group obviously decreased (5.90%, P < 0.01). The expression rates of NF-kappaB p65 in the ECR group (13.99%) and the E2 group (25.18%) ranged between the control group and the H2O2 group, but still lower than that of the H2O2 group (P < 0.01).
  
  
  CONCLUSIONS
  The activation of NF-kappaB in the HLECs could be induced by H2O2 ECR with the estrogenic activity could effectively inhibit the activation of NF-kappaB.
authors.affiliation: Department of Ophthalmology, Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Feng
authors.forename: Xiu-Rong
authors.initials: XR
authors.lastname: Huang
authors.forename: Ming-Xin
authors.initials: MX
authors.lastname: Qi
mesh_terms: D002478:Cells, Cultured; D004441:Ecdysterone / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects; D007908:Lens, Crystalline / Q000166:cytology; D018384:Oxidative Stress / Q000187:drug effects*; D051996:Transcription Factor RelA / Q000378:metabolism*
chemical_list: C498143:RELA protein, human; D051996:Transcription Factor RelA; D004441:Ecdysterone; D006861:Hydrogen Peroxide