title: Isolation of brefeldin A from Eupenicillium brefeldianum broth using macroporous resin adsorption chromatography.
abstract: Brefeldin A (BFA) is a macrolide lactone antibiotic, possessing antitumor, antiviral, antifungal activities. In this work, a separation strategy involving one-step macroporous resin adsorption chromatography combined with crystallization was established for BFA purification from Eupenicillium brefeldianum CCTCC M 208113 fermentation broth. Among six macroporous resin adsorbents tested, the non-polar resin HZ830 had the best adsorption and desorption performance. The static equilibrium adsorption data fitted well with the Freundlich equation, and the adsorption kinetic followed the pseudo-second order model. Through experimental optimization of column adsorption and desorption, BFA in purity of 90.4% (w/w), 92.1% (w/w) yield was obtained by a one-step macroporous resin adsorption chromatography, using a stepwise elution protocol. Furthermore, high purity (>99%, w/w) of BFA crystals were prepared from E. brefeldianum CCTCC M 208113 fermentation broth in an overall recovery of 67.0% (w/w), using a combination of adsorption chromatography packed with non-polar macroporous adsorbent HZ830 and crystallization in acetone.
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Ye-Fei
authors.initials: YF
authors.lastname: Wu
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Zhi-Xian
authors.initials: ZX
authors.lastname: Wu
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Xue
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
authors.forename: Yin-Chu
authors.initials: YC
authors.lastname: Shen
mesh_terms: D000327:Adsorption; D020126:Brefeldin A / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D017077:Culture Media, Conditioned; D004058:Diffusion; D000431:Ethanol; D055324:Eupenicillium / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D007700:Kinetics; D019995:Laboratory Chemicals / Q000737:chemistry; D016014:Linear Models; D014867:Water
chemical_list: D017077:Culture Media, Conditioned; D019995:Laboratory Chemicals; D014867:Water; D020126:Brefeldin A; D000431:Ethanol