title: Continuous-flow reactor-based enzymatic synthesis of phosphorylated compounds on a large scale.
abstract: Acid phosphatase, an enzyme that is able to catalyze the transfer of a phosphate group from cheap pyrophosphate to alcoholic substrates, was covalently immobilized on polymethacrylate beads with an epoxy linker (Immobeads-150 or Sepabeads EC-EP). After immobilization 70% of the activity was retained and the immobilized enzyme was stable for many months. With the immobilized enzyme we were able to produce and prepare D-glucose-6-phosphate, N-acetyl-D-glucosamine-6-phosphate, allyl phosphate, dihydroxyacetone phosphate, glycerol-1-phosphate, and inosine-5'-monophosphate from the corresponding primary alcohol on gram scale using either a fed-batch reactor or a continuous-flow packed-bed reactor.
authors.affiliation: Van't Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands.
authors.forename: Lara
authors.initials: L
authors.lastname: Babich
authors.forename: Aloysius F
authors.initials: AF
authors.lastname: Hartog
authors.forename: Michael A
authors.initials: MA
authors.lastname: van der Horst
authors.forename: Ron
authors.initials: R
authors.lastname: Wever
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D000135:Acid Phosphatase / Q000378:metabolism*; D002384:Catalysis; D019298:Glucose-6-Phosphate / Q000138:chemical synthesis / Q000737:chemistry; D007291:Inosine Monophosphate / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry
chemical_list: D009943:Organophosphorus Compounds; D011109:Polymethacrylic Acids; D007291:Inosine Monophosphate; C010004:N-acetylglucosamine 6-phosphate; D019298:Glucose-6-Phosphate; D000135:Acid Phosphatase; D000117:Acetylglucosamine