title: Nitric oxide is responsible for oxidative skin injury and modulation of cell proliferation after 24 hours of UVB exposures.
abstract: Nitric oxide (NO) is produced by various mammalian cells and plays a variety of regulatory roles in normal physiology and in pathological processes. This article provides evidence regarding the participation of NO in UVB-induced skin lesions and in the modulation of skin cell proliferation following UVB skin irradiation. Hairless mice were subjected to UVB irradiation for 3 hours and the skin evaluated immediately, 6 and 24 hours postirradiation. The skin lipid peroxidation, and NO levels evaluated by chemiluminescence and inducible nitric oxide synthase (iNOS) and nitrotyrosine immunolabelling increased significantly 24 hours after irradiation and decreased under the treatment with aminoguanidine (AG). On the other hand, cell proliferation markers, PCNA and VEGF showed a strong labelling index when AG was used. The data indicate that NO mediates, at least in part, the lipid peroxidation and protein nitration and also promotes the down regulation of factors involved in cell proliferation. This work shows that the NO plays an important role in the oxidative stress damage and on modulation of cell proliferation pathways in UVB irradiated skin.
authors.affiliation: Laboratorio de Patologia Molecular, Universidade Estadual de Londrina. Rod . Celso Garcia Cid, PR-445, km 380, Londrina, PR, Brasil.
authors.forename: Vania Aparecida
authors.initials: VA
authors.lastname: Terra
authors.forename: Fernando Pereira
authors.initials: FP
authors.lastname: Souza-Neto
authors.forename: Raissa Caroline
authors.initials: RC
authors.lastname: Pereira
authors.forename: Thamara Nishida
authors.initials: TN
authors.lastname: Xavier Da Silva
authors.forename: Leandra Naira Zambelli
authors.initials: LN
authors.lastname: Ramalho
authors.forename: Rodrigo Cabral
authors.initials: RC
authors.lastname: Luiz
authors.forename: Rubens
authors.initials: R
authors.lastname: Cecchini
authors.forename: Alessandra Lourenco
authors.initials: AL
authors.lastname: Cecchini
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D006146:Guanidines / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis / Q000276:immunology; D014466:Ultraviolet Rays / Q000009:adverse effects; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D004791:Enzyme Inhibitors; D006146:Guanidines; D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D052247:Nitric Oxide Synthase Type II; C004479:pimagedine