title: Locally resolved membrane binding affinity of the N-terminus of α-synuclein.
abstract: α-Synuclein is abundantly present in Lewy bodies, characteristic of Parkinson's disease. Its exact physiological role has yet to be determined, but mitochondrial membrane binding is suspected to be a key aspect of its function. Electron paramagnetic resonance spectroscopy in combination with site-directed spin labeling allowed for a locally resolved analysis of the protein-membrane binding affinity for artificial phospholipid membranes, supported by a study of binding to isolated mitochondria. The data reveal that the binding affinity of the N-terminus is nonuniform.
authors.affiliation: Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, 78457 Konstanz, Germany.
authors.forename: Marta
authors.initials: M
authors.lastname: Robotta
authors.forename: Christian
authors.initials: C
authors.lastname: Hintze
authors.forename: Stefan
authors.initials: S
authors.lastname: Schildknecht
authors.forename: Niels
authors.initials: N
authors.lastname: Zijlstra
authors.forename: Christian
authors.initials: C
authors.lastname: Jüngst
authors.forename: Christiaan
authors.initials: C
authors.lastname: Karreman
authors.forename: Martina
authors.initials: M
authors.lastname: Huber
authors.forename: Marcel
authors.initials: M
authors.lastname: Leist
authors.forename: Vinod
authors.initials: V
authors.lastname: Subramaniam
authors.forename: Malte
authors.initials: M
authors.lastname: Drescher
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D016631:Lewy Bodies / Q000378:metabolism; D008567:Membranes, Artificial; D009154:Mutation; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000737:chemistry / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism*
chemical_list: D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010743:Phospholipids; D051844:alpha-Synuclein; C060037:1-palmitoyl-2-oleoylglycero-3-phosphoglycerol; C028694:1-palmitoyl-2-oleoylphosphatidylcholine