title: Pronounced allelic imbalance at D9S162 in skin squamous cell carcinoma of organ transplant recipients.
abstract: OBJECTIVE
  To evaluate chromosomal instability at 9p21-22 with p16 protein expression in organ transplant recipients (OTRs) compared with immunocompetent patients with squamous cell carcinoma (SCC).
  
  
  METHODS
  In a select population of intraepithelial and subsequent invasive SCC from the same anatomic region of the same patient at different times, we assessed loss of heterozygosity at 3 microsatellites—IFNA, D9S162, and D9S925—in the course of carcinogenesis in OTRs and immunocompetent patients.
  
  
  METHODS
  Department of Dermatology, University Hospital Zurich.
  
  
  METHODS
  Immunocompetent patients and OTRs with SCC on sun-damaged skin.
  
  
  METHODS
  Chromosomal allelic balance in SCC of OTRs and immunocompetent patients.
  
  
  RESULTS
  Reduced allelic balance at IFNA, D9S162, and D9S925 in intraepithelial forms of SCC and similar allelic imbalance in invasive forms of SCC were found. Allelic balance at D9S162 was reduced for SCC in OTRs compared with SCC in immunocompetent patients. The study revealed broadly reduced allelic balance at 9p21-22 in all cutaneous SCCs, and OTRs presented a further reduced allelic balance for D9S162, suggesting a common trait for SCC in OTRs. Actinic keratosis and Bowen disease differed in allelic balance at D9S162, suggesting substantial differences in their carcinogenesis.
  
  
  CONCLUSIONS
  Reduced allelic balance around locus D9S162 is a genomic correlate for enhanced carcinogenesis in OTRs.
authors.affiliation: Department of Dermatology, University Hospital Zurich, Zurich, Switzerland.
authors.forename: Beda
authors.initials: B
authors.lastname: Mühleisen
authors.forename: Ivaylo
authors.initials: I
authors.lastname: Petrov
authors.forename: Simona
authors.initials: S
authors.lastname: Frigerio
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziunycz
authors.forename: Lars E
authors.initials: LE
authors.lastname: French
authors.forename: Günther F L
authors.initials: GF
authors.lastname: Hofbauer
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D043171:Chromosomal Instability / Q000235:genetics*; D005260:Female; D006785:Hospitals, University; D006801:Humans; D007121:Immunocompetence; D016867:Immunocompromised Host; D019656:Loss of Heterozygosity / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D016377:Organ Transplantation; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D013557:Switzerland