title: The effect of maize silage as co-substrate for swine manure on the bacterial community structure in biogas plants.
abstract: The qualitative and quantitative changes in the bacterial community composition in two mesophilic, commercially used biogas plants were monitored by denaturing gradient gel electrophoresis (DGGE) and real-time PCR. The main objective was to evaluate the influence of the co-substrate maize silage on total bacteria and some selected bacterial groups by comparing full-scale reactors fed solely with pig manure or additionally with maize silage. DGGE fingerprints reflected shifts in the bacterial community structure associated with maize silage as co-substrate and the real-time PCR results showed clear changes in the quantitative composition of the bacterial consortia of each fermenter. A clear dominance of Clostridia in all surveyed fermenters and considerably lower abundance of Bacteroidetes in the biogas plant fed with maize silage was shown.
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic. fliegerova@iapg.cas.cz
authors.forename: K
authors.initials: K
authors.lastname: Fliegerová
authors.forename: J
authors.initials: J
authors.lastname: Mrázek
authors.forename: M
authors.initials: M
authors.lastname: Kajan
authors.forename: S M
authors.initials: SM
authors.lastname: Podmirseg
authors.forename: H
authors.initials: H
authors.lastname: Insam
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D044822:Biodiversity; D056804:Biofuels / Q000032:analysis / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media / Q000032:analysis / Q000378:metabolism; D008372:Manure / Q000032:analysis / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012820:Silage / Q000032:analysis / Q000382:microbiology*; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology*
chemical_list: D056804:Biofuels; D003470:Culture Media; D008372:Manure