title: Highly selective trapping of enteropathogenic E. coli on Fabry-Pérot sensor mirrors.
abstract: Untreated recycled water, such as sewage and graywater, will almost always contain a wide range of agents that are likely to present risks to human health, including chemicals and pathogenic microorganisms. The microbial hazards, such as large numbers of enteric pathogens that can cause gastroenteric illness if ingested, are the main cause of concern for human health. The presence of the enteropathogenic Escherichia coli (EPEC) serotype is of particular concern, as this group of bacteria is responsible for causing severe infant and travelers' diarrhea, gastroenteritis and hemolytic uremic syndrome. A biosensing system based on an optical Fabry-Pérot (FP) cavity, capable of directly detecting the presence of EPEC within 5 min, has been developed using a simple micro-thin double-sided adhesive tape and two semi-transparent FP mirror plates. The system utilizes a poly(methyl methacrylate) (PMMA) or glass substrates sputtered by 40-nm-thick gold thin films serving as FP mirrors. Mirrors have been activated using 0.1M mercaptopropionic acid, influencing an immobilization density of the translocated intimin receptor (TIR) of 100 ng/cm(2). The specificity of recognition was confirmed by exposing TIR functionalized surfaces to four taxonomically related and/or distantly related bacterial strains. It was found that the TIR-functionalized surfaces did not show any bacterial capture for these other bacterial strains within a 15 min incubation period.
authors.affiliation: Faculty of Life and Social Sciences, Swinburne University of Technology, PO Box 218, Hawthorn 3122, Australia. Electronic address: eivanova@swin.edu.au.
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova
authors.affiliation: Faculty of Life and Social Sciences, Swinburne University of Technology, PO Box 218, Hawthorn 3122, Australia.
authors.forename: Vi Khanh
authors.initials: VK
authors.lastname: Truong
authors.affiliation: Centre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, PO Box 218, Hawthorn 3122, Australia.
authors.forename: Gediminas
authors.initials: G
authors.lastname: Gervinskas
authors.affiliation: Faculty of Life and Social Sciences, Swinburne University of Technology, PO Box 218, Hawthorn 3122, Australia.
authors.forename: Natasa
authors.initials: N
authors.lastname: Mitik-Dineva
authors.affiliation: Centre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, PO Box 218, Hawthorn 3122, Australia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Day
authors.affiliation: Centre for Materials and Surface Science, Department of Physics, La Trobe University, Victoria 3086, Australia.
authors.forename: Robert T
authors.initials: RT
authors.lastname: Jones
authors.affiliation: Faculty of Life and Social Sciences, Swinburne University of Technology, PO Box 218, Hawthorn 3122, Australia.
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Crawford
authors.affiliation: Centre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, PO Box 218, Hawthorn 3122, Australia; Melbourne Centre for Nanofabrication, 151 Wellington Road, Clayton, Victoria 3168, Australia.
authors.forename: Saulius
authors.initials: S
authors.lastname: Juodkazis
mesh_terms: D001422:Bacterial Adhesion; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D054308:Enteropathogenic Escherichia coli / Q000302:isolation & purification*; D004867:Equipment Design; D019453:Escherichia coli O157 / Q000302:isolation & purification; D029968:Escherichia coli Proteins; D005898:Glass; D006046:Gold; D006801:Humans; D055767:Immobilized Proteins; D055096:Optical Devices; D019904:Polymethyl Methacrylate; D011956:Receptors, Cell Surface; D012722:Sewage / Q000382:microbiology; D013045:Species Specificity; D013499:Surface Properties; D014871:Water Microbiology
chemical_list: D029968:Escherichia coli Proteins; D055767:Immobilized Proteins; D011956:Receptors, Cell Surface; D012722:Sewage; C109287:Tir protein, E coli; D006046:Gold; D019904:Polymethyl Methacrylate