title: A "neural" enzyme in nonbilaterian animals and algae: preneural origins for peptidylglycine α-amidating monooxygenase.
abstract: Secreted peptides, produced by enzymatic processing of larger precursor molecules, are found throughout the animal kingdom and play important regulatory roles as neurotransmitters and hormones. Many require a carboxy-terminal modification, involving the conversion of a glycine residue into an α-amide, for their biological activity. Two sequential enzymatic activities catalyze this conversion: a monooxygenase (peptidylglycine α-hydroxylating monooxygenase or PHM) and an amidating lyase (peptidyl-α-hydroxyglycine α-amidating lyase or PAL). In vertebrates, these activities reside in a single polypeptide known as peptidylglycine α-amidating monooxygenase (PAM), which has been extensively studied in the context of neuropeptide modification. Bifunctional PAMs have been reported from some invertebrates, but the phylogenetic distribution of PAMs and their evolutionary relationship to PALs and PHMs is unclear. Here, we report sequence and expression data for two PAMs from the coral Acropora millepora (Anthozoa, Cnidaria), as well as providing a comprehensive survey of the available sequence data from other organisms. These analyses indicate that bifunctional PAMs predate the origins of the nervous and endocrine systems, consistent with the idea that within the Metazoa their ancestral function may have been to amidate epitheliopeptides. More surprisingly, the phylogenomic survey also revealed the presence of PAMs in green algae (but not in higher plants or fungi), implying that the bifunctional enzyme either predates the plant/animal divergence and has subsequently been lost in a number of lineages or perhaps that convergent evolution or lateral gene transfer has occurred. This finding is consistent with recent discoveries that other molecules once thought of as "neural" predate nervous systems.
authors.affiliation: Evolution, Ecology and Genetics, Research School of Biology, Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Rosalind M F
authors.initials: RM
authors.lastname: Attenborough
authors.forename: David C
authors.initials: DC
authors.lastname: Hayward
authors.forename: Marcelo V
authors.initials: MV
authors.lastname: Kitahara
authors.forename: David J
authors.initials: DJ
authors.lastname: Miller
authors.forename: Eldon E
authors.initials: EE
authors.lastname: Ball
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D019760:Amidine-Lyases / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D037421:Anthozoa / Q000201:enzymology* / Q000235:genetics; D055162:Biocatalysis; D000460:Chlorophyta / Q000201:enzymology*; D019143:Evolution, Molecular; D015971:Gene Expression Regulation, Enzymologic; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000201:enzymology*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
chemical_list: D009097:Multienzyme Complexes; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase; D019760:Amidine-Lyases; C068521:peptidylamidoglycolate lyase