title: Fluorescently labeled branched polymers and thermal responsive nanoparticles for live cell imaging.
abstract: Branched poly(methoxy-PEG acrylate) and thermally responsive poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) are synthesized by RAFT polymerization. After reduction, these polymers are fluorescently labeled by reacting the free thiol groups with N-(5-fluoresceinyl)maleimide. As shown by DLS, the labeled copolymer poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) forms nanoparticles at body temperature (37 °C) due to the presence of the thermosensitive poly(N-isopropylacrylamide). These materials were used as bioprobes for imaging HUVECs in vitro and chick embryo CAM in vivo. Both labeled polymer and nanoparticles are biocompatible and can be used as efficient fluorescent bioprobes.
authors.affiliation: Department of Polymer Chemistry and Biomaterials, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands.
authors.lastname: Di Zhou
authors.forename: Yujie
authors.initials: Y
authors.lastname: Ma
authors.forename: André A
authors.initials: AA
authors.lastname: Poot
authors.forename: Pieter J
authors.initials: PJ
authors.lastname: Dijkstra
authors.forename: Jan
authors.initials: J
authors.lastname: Feijen
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis* / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D008301:Maleimides / Q000737:chemistry; D057054:Molecular Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000494:pharmacology; D011108:Polymers / Q000138:chemical synthesis* / Q000494:pharmacology; D013194:Staining and Labeling; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D005456:Fluorescent Dyes; D008301:Maleimides; D011108:Polymers; C574088:poly(methoxy-polyethylene glycol acrylate); C574089:poly(methoxy-polyethylene glycol acrylate)-block-poly(N-isopropylacrylamide); D011092:Polyethylene Glycols