title: Monoclonal antibodies against recombinant AtHOS15.
abstract: Histone modifications are important components of transcriptional regulation and chromatin-based regulatory processes. In addition, WD40-repeat protein and several other components are involved in these functions. Here we present the development of monoclonal antibodies (MAbs) against Arabidopsis HOS15, a WD40-repeat protein. Mice were immunized with recombinant HOS15 (rHOS15) protein for generating MAbs via the classic hybridoma production technique. We confirmed the specific activity of anti-HOS15 MAbs by tobacco transient expression assays. Based on immunoprecipitation assays, the anti-HOS15 MAb was able to detect endogenous HOS15 in Arabidopsis wild-type plants, but not in hos15 mutant plants. Finally, the anti-HOS15 MAbs are highly sensitive for detecting endogenous HOS15 protein. They can be used for immunological and immunoprecipitation assays to support other experimental strategies. In particular, the anti-HOS15 MAbs will be essential tools to investigate the role of HOS15 in the regulation of tolerance to environmental stresses in plants.
authors.affiliation: Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Gwangjin-gu, Seoul, Korea.
authors.forename: Siyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Hyeong Cheol
authors.initials: HC
authors.lastname: Park
authors.forename: Suyoung
authors.initials: S
authors.lastname: Bae
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Hong
authors.forename: Jida
authors.initials: J
authors.lastname: Choi
authors.forename: Kwangwon
authors.initials: K
authors.lastname: Hong
authors.forename: Hyunjhung
authors.initials: H
authors.lastname: Jhun
authors.forename: Kangchang
authors.initials: K
authors.lastname: Kim
authors.forename: Eusom
authors.initials: E
authors.lastname: Kim
authors.forename: Seounghyun
authors.initials: S
authors.lastname: Jo
authors.forename: Woe-Yeon
authors.initials: WY
authors.lastname: Kim
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
authors.forename: Soohyun
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D029681:Arabidopsis Proteins; D002868:Chromosomal Proteins, Non-Histone; C528184:Hos15 protein, Arabidopsis; D011994:Recombinant Proteins