title: Design of polydiacetylene-phospholipid supramolecules for enhanced stability and sensitivity.
abstract: We present polydiacetylene (PDA) liposome assemblies with various phospholipids that have different headgroup charges and phase transition temperatures (T(m)). 10,12-Pentacosadiynoic acid (PCDA)-epoxy was used as a base PDA monomer and the insertion of highly charged phospholipids resulted in notable changes in the size of liposome and reduction of the aggregation of PDA liposome. Among the various phospholipids, the phospholipid with a moderate T(m) demonstrated enhanced stability and sensitivity, as measured by the size and zeta potential over storage time, thermochoromic response, and transmission electron microscopy images. By combining these results, we were able to detect immunologically an antibody of bovine viral diarrhea virus over a wide dynamic range of 0.001 to 100 μg/mL.
authors.affiliation: School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151-742, Korea.
authors.forename: Do Hyun
authors.initials: DH
authors.lastname: Kang
authors.forename: Ho-Sup
authors.initials: HS
authors.lastname: Jung
authors.forename: Jiseok
authors.initials: J
authors.lastname: Lee
authors.forename: Sungbaek
authors.initials: S
authors.lastname: Seo
authors.forename: Jinsang
authors.initials: J
authors.lastname: Kim
authors.forename: Keesung
authors.initials: K
authors.lastname: Kim
authors.forename: Kahp-Yang
authors.initials: KY
authors.lastname: Suh
mesh_terms: D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D010743:Phospholipids / Q000737:chemistry*; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*
chemical_list: C493048:10, 12-pentacosadiynoic acid; D005231:Fatty Acids, Unsaturated; D008081:Liposomes; D010743:Phospholipids; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene