title: Production of protocatechuic acid in Bacillus Thuringiensis ATCC33679.
abstract: Protocatechuic acid, or 3,4-dihydroxybenzoic acid, is produced by both soil and marine bacteria in the free form and as the iron binding component of the siderophore petrobactin. The soil bacterium, Bacillus thuringiensis kurstaki ATCC 33679, contains the asb operon, but does not produce petrobactin. Iron restriction resulted in diminished B. thuringiensis kurstaki ATCC 33679 growth and the production of catechol(s). The gene product responsible for protocatechuic acid (asbF) and its receptor (fatB) were expressed during stationary phase growth. Gene expression varied with growth temperature, with optimum levels occurring well below the Bacillus anthracis virulence temperature of 37 °C. Regulation of protocatechuic acid suggests a possible role for this compound during soil growth cycles.
authors.affiliation: Department of Biology, Tougaloo College, Tougaloo, MS 39174, USA.
authors.forename: Kimtrele M
authors.initials: KM
authors.lastname: Williams
authors.affiliation: Department of Biology, Tougaloo College, Tougaloo, MS 39174, USA.
authors.forename: William E
authors.initials: WE
authors.lastname: Martin
authors.affiliation: Department of Biology, Tougaloo College, Tougaloo, MS 39174, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Smith
authors.affiliation: Department of Biology, Tougaloo College, Tougaloo, MS 39174, USA.|Center for Bioinformatics and Computational Biology, Department of Biology, Jackson State University, Jackson, MS 39217, USA.
authors.forename: Baraka S
authors.initials: BS
authors.lastname: Williams
authors.affiliation: Department of Biology, Tougaloo College, Tougaloo, MS 39174, USA.
authors.forename: Bianca L
authors.initials: BL
authors.lastname: Garner
mesh_terms: D016588:Anticarcinogenic Agents / Q000378:metabolism; D001413:Bacillus thuringiensis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism*; D015870:Gene Expression; D062385:Hydroxybenzoates / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000494:pharmacology; D000090463:Iron Deficiencies; D017262:Siderophores; D013696:Temperature
chemical_list: D016588:Anticarcinogenic Agents; D002396:Catechols; D062385:Hydroxybenzoates; D007502:Iron Chelating Agents; D017262:Siderophores; C009091:protocatechuic acid