title: Assessment on pollution of Ochratoxin A in grain in China and its apoptosis effect on vitro-cultured human tubular kidney cells.
abstract: Ochratoxin A (OTA) is nephrotoxic, immunosuppressive, and teratogenic in many species and is a possible human carcinogen. In this study, we investigated the OTA pollution situations of grains in northern China and the signaling pathway that mediated OTA-induced apoptosis in human tubular kidney cells (HKCs). Samples of grains collected from three representative areas were determined by using high-performance liquid chromatography fluorescence method. The effects of OTA on cell apoptosis, caspase-3, Bax, and Bcl-2 expression, and phosphorylation of c-Jun NH(2) terminal kinase (JNK) were detected in cultured HKCs via flow cytometry (FCM), Hoechst 33258 staining, and Western blot. It showed that OTA pollution of edible grains was very common in north China. OTA could affect caspase-3, Bax, and Bcl-2 expression and increased cell apoptosis in cultured HKCs. The JNK signalling pathway might play an important role during these cellular events.
authors.affiliation: Department of Nutrition, The First Attached Hospital, Hebei Medical University, Shijiazhuang, People's Republic of China. zengningli@hebmu.edu.cn
authors.forename: Zengning
authors.initials: Z
authors.lastname: Li
authors.forename: Xianghong
authors.initials: X
authors.lastname: Zhang
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Cui
authors.forename: Weijun
authors.initials: W
authors.lastname: Kang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D002681:China; D002523:Edible Grain / Q000737:chemistry*; D004785:Environmental Pollutants / Q000302:isolation & purification / Q000633:toxicity*; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D009793:Ochratoxins / Q000302:isolation & purification / Q000633:toxicity*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D009793:Ochratoxins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C025589:ochratoxin A; D048031:JNK Mitogen-Activated Protein Kinases; D053148:Caspase 3