title: Phylogenetics of the millipede genus Brachycybe Wood, 1864 (Diplopoda: Platydesmida: Andrognathidae): patterns of deep evolutionary history and recent speciation.
abstract: The genus Brachycybe Wood is a little known group of millipedes comprising eight nominal species distributed throughout North America, Japan, South Korea, Taiwan, and China. The group's species are relatively morphologically homogenous and have been described primarily on the basis of differences in somatic morphology largely ignoring the often-used characters in millipede taxonomy and systematics - male genitalia (the gonopods). The objectives of this study were to survey male gonopods with the aim of evaluating inter-specific variation, assess existing species boundaries and phylogeny using molecular characters, examine the historical biogeography of the genus, and estimate the timing of lineage divergence using a molecular clock. We surveyed two mitochondrial genes (cytochrome c oxidase I and cytochrome b) and one nuclear protein-coding gene (glutamyl and prolyl-tRNA synthetase) each comprising 952, 746, and 555 aligned base pairs respectively. Phylogenetic inference coupled with an analysis of species delimitation using a generalized mixed Yule coalescent model recovered eight species, two of which were considered to be cryptic. Molecular dating analyses coupled with ancestral range reconstructions indicate that the group is quite ancient (age>50 million years) and its origins are likely traced back geographically to the mountains of California's Sierra Nevada and Coastal Ranges. The genus Brachycybe appears to have expanded its range at least twice out of present day California into eastern North America and at least once into Asia. This study highlights the need for integrative approaches to describe biodiversity and furthers the evidence for cryptic diversity even in groups where genitalia are generally thought to be rapidly evolving, diagnostic features.
authors.affiliation: Department of Biology, East Carolina University, Greenville, NC 27858, USA.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Brewer
authors.forename: Chad L
authors.initials: CL
authors.lastname: Spruill
authors.forename: Nandita S
authors.initials: NS
authors.lastname: Rao
authors.forename: Jason E
authors.initials: JE
authors.lastname: Bond
mesh_terms: D000818:Animals; D001181:Arthropods / Q000033:anatomy & histology* / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D001499:Bayes Theorem; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D003710:Demography; D019143:Evolution, Molecular; D005202:Asia, Eastern; D049810:Genetic Speciation; D005837:Genitalia, Male / Q000648:ultrastructure*; D005975:Glutamate-tRNA Ligase / Q000235:genetics; D016013:Likelihood Functions; D008297:Male; D008855:Microscopy, Electron, Scanning; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009656:North America; D010802:Phylogeny; D058974:Phylogeography; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D005975:Glutamate-tRNA Ligase