title: ParA-like protein uses nonspecific chromosomal DNA binding to partition protein complexes.
abstract: Recent data have shown that plasmid partitioning Par-like systems are used by some bacterial cells to control localization of protein complexes. Here we demonstrate that one of these homologs, PpfA, uses nonspecific chromosome binding to separate cytoplasmic clusters of chemotaxis proteins upon division. Using fluorescent microscopy and point mutations, we show dynamic chromosome binding and Walker-type ATPase activity are essential for cluster segregation. The N-terminal domain of a cytoplasmic chemoreceptor encoded next to ppfA is also required for segregation, probably functioning as a ParB analog to control PpfA ATPase activity. An orphan ParA involved in segregating protein clusters therefore uses a similar mechanism to plasmid-segregating ParA/B systems and requires a partner protein for function. Given the large number of genomes that encode orphan ParAs, this may be a common mechanism regulating segregation of proteins and protein complexes.
authors.affiliation: Oxford Centre for Integrative Systems Biology, University of Oxford, Oxford OX1 3QU, United Kingdom.
authors.forename: Mark A J
authors.initials: MA
authors.lastname: Roberts
authors.forename: George H
authors.initials: GH
authors.lastname: Wadhams
authors.forename: Katie A
authors.initials: KA
authors.lastname: Hadfield
authors.forename: Susan
authors.initials: S
authors.lastname: Tickner
authors.forename: Judith P
authors.initials: JP
authors.lastname: Armitage
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D017354:Point Mutation; D012242:Rhodobacter sphaeroides / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial