title: Purification and characterization of a lectin of the Swartzieae Legume Taxa.
abstract: This work aimed at describing the first biochemical and structural data of a lectin belonging to Swartzieae, a primitive Legume Taxa. A lactose-binding seed lectin (SLL) was purified by affinity chromatography of crude saline extracts of Swartzia laevicarpa on immobilized lactose. The SLL agglutinated rabbit erythrocytes but not rat or human (A, B, O) erythrocytes. Lectin activity was retained after heating at 100 �C for 15 min and was best inhibited by Nacetylgalactosamine, lactose and galactose. The lectin exhibited a single electrophoretic pattern that corresponded to a molecular mass of 29,000 Da, which was confirmed by MS analysis. In addition, the lectin reacted positively with Schiff's reagent. The unique N-terminal amino acid sequence (39 residues) and the internal peptide sequence were determined by Edman degradation and MS/MS, respectively. The sequencing revealed complete homology of the SLL with legume lectins belonging to primitive groups (Dalbergieae and Sophoreae). The SLL (at 1 mg/ml) did not exhibit antifungal activity against various phytopathogens or cytotoxicity (at 100 μg/ml) towards different cancer cell lines.
authors.affiliation: National Institute for Research in the Amazon (MCTI-INPA), Laboratory of Plant Physiology and Biochemistry, Manaus, Amazonas, Brazil.
authors.forename: Andreia Varmes
authors.initials: AV
authors.lastname: Fernandes
authors.forename: Márcio Viana
authors.initials: MV
authors.lastname: Ramos
authors.forename: Ilka Maria
authors.initials: IM
authors.lastname: Vasconcelos
authors.forename: Ana Cristina Oliveira Monteiro
authors.initials: AC
authors.lastname: Moreira
authors.forename: Frederico Bruno
authors.initials: FB
authors.lastname: Moreno
authors.forename: Jose Odair
authors.initials: JO
authors.lastname: Pereira
authors.forename: José Francisco
authors.initials: JF
authors.lastname: de Carvalho Gonçalves
mesh_terms: D000116:Acetylgalactosamine / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D016923:Cell Death; D002846:Chromatography, Affinity; D004586:Electrophoresis; D007887:Fabaceae / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects*; D005690:Galactose / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects*; D006386:Hemagglutination Tests; D006801:Humans; D007785:Lactose / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D010446:Peptide Fragments / Q000494:pharmacology*; D037121:Plant Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D012639:Seeds / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured
chemical_list: D010446:Peptide Fragments; D037121:Plant Lectins; D007785:Lactose; D000116:Acetylgalactosamine; D005690:Galactose