title: Topical hesperidin improves epidermal permeability barrier function and epidermal differentiation in normal murine skin.
abstract: Orange peel extract appears to exhibit beneficial effects on skin whitening, inflammation, UVB protection, as well as keratinocyte proliferation. In the present study, we determine whether topical hesperidin influences epidermal permeability barrier function and its underlying mechanisms. Hairless mice were treated topically with 2% hesperidin or 70% ethanol alone twice daily for 6 days. At the end of treatment, basal transepidermal water loss (TEWL) was measured 2 and 4 h post barrier disruption. Epidermal proliferation and differentiation were evaluated by immunohistochemical staining and Western blot analysis. Additionally, lamellar body density and secretion were assessed by electron microscopy. Although there were no significant differences in basal barrier function, in comparison with control animals, topical hesperidin significantly accelerated barrier recovery at both 2 and 4 h after acute barrier abrogation. Enhanced barrier function in hesperidin-treated skin correlated with stimulation of both epidermal proliferation and differentiation, as well as enhanced lamellar body secretion. These results indicate that topical hesperidin enhances epidermal permeability barrier homeostasis at least in part due to stimulation of epidermal proliferation, differentiation, as well as lamellar body secretion.
authors.affiliation: Department of Dermatology, Nanjing Medical University, Nanjing, PR China.
authors.forename: Maihua
authors.initials: M
authors.lastname: Hou
authors.forename: Mona
authors.initials: M
authors.lastname: Man
authors.forename: Wenyan
authors.initials: W
authors.lastname: Man
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Zhu
authors.forename: Melanie
authors.initials: M
authors.lastname: Hupe
authors.forename: Kyungho
authors.initials: K
authors.lastname: Park
authors.forename: Debra
authors.initials: D
authors.lastname: Crumrine
authors.forename: Peter M
authors.initials: PM
authors.lastname: Elias
authors.forename: Mao-Qiang
authors.initials: MQ
authors.lastname: Man
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001706:Biopsy; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000078404:Epidermal Cells; D004817:Epidermis / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D006569:Hesperidin / Q000008:administration & dosage* / Q000494:pharmacology*; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008812:Mice, Hairless; D023421:Models, Animal
chemical_list: D006569:Hesperidin