title: Phenylboronic-acid-modified amphiphilic polyether as a neutral gene vector.
abstract: A phenylboronic-acid-modified amphiphilic block polyether is prepared via reaction of polyglycidol-block-poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide)-block-polyglycidol (Pluronic-PG) with 2-(N,N-dimethylaminomethyl)-5-aminomethyl phenylboronic acid using phosgene as a coupling reagent. The boronic-acid-modified non-cationic polymer binds plasmid pGL3 effectively, forms sub-µm polymer/DNA complex particles, and greatly facilitates the cell uptake of the plasmid. The efficiency of the polymer as a gene vector is evaluated in vitro by transfection of pGL3 to HeLa, COS-7 and HepG2 cells. Pluronic-PG-BA enhances the transfection efficiency by 100 to 1000 times compared with Pluronic-PG. The presence of serum does not significantly affect the transfection efficiency.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors.forename: Fujie
authors.initials: F
authors.lastname: Chen
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Cai
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhenlin
authors.initials: Z
authors.lastname: Zhong
authors.forename: Renxi
authors.initials: R
authors.lastname: Zhuo
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D019556:COS Cells; D002470:Cell Survival; D002522:Chlorocebus aethiops; D004247:DNA; D004987:Ethers / Q000737:chemistry*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D010705:Phosgene / Q000737:chemistry; D010957:Plasmids; D020442:Poloxamer / Q000737:chemistry*; D058105:Polymerization; D013501:Surface-Active Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D014162:Transfection
chemical_list: D001897:Boronic Acids; D004987:Ethers; D013501:Surface-Active Agents; D020442:Poloxamer; D010705:Phosgene; D004247:DNA; C010686:benzeneboronic acid