title: Glycodelin suppresses endometrial cell migration and invasion but stimulates spheroid attachment.
abstract: Glycodelin contains four isoforms with diverse biological functions. Glycodelin-A is potentially a diagnostic marker for cancer patients and receptivity marker of the secretory endometrium. Yet, direct evidence for the role of glycodelin in the regulation of endometrial epithelial cell migration, invasion and attachment of trophoblastic spheroids (blastocyst surrogate) is lacking. In this study, the human glycodelin gene was stably transfected into human endometrial (HEC1-B) cells. Forced expression of glycodelin in HEC1-B cells did not affect cell proliferation, cell viability or cell-cycle progression, but significantly reduced migration and invasion of the stably transfected cells (both P<0.05). The migration rate returned to normal levels when the glycodelin-forced-expressing HEC1-B cells were treated with glycodelin RNAi. Furthermore, forced expression of glycodelin in HEC1-B cells significantly increased the attachment of trophoblastic spheroids onto the endometrial epithelial cells (P<0.05). In summary, glycodelin suppressed endometrial cell migration and invasion but enhanced spheroid attachment.
authors.affiliation: Department of Obstetrics and Gynaecology, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
authors.forename: Kam-Hei
authors.initials: KH
authors.lastname: So
authors.forename: Cheuk-Lun
authors.initials: CL
authors.lastname: Lee
authors.forename: William S B
authors.initials: WS
authors.lastname: Yeung
authors.forename: Kai-Fai
authors.initials: KF
authors.lastname: Lee
mesh_terms: D018269:Carcinoma, Endometrioid / Q000473:pathology; D002453:Cell Cycle / Q000502:physiology; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D010064:Embryo Implantation; D016889:Endometrial Neoplasms / Q000473:pathology; D004717:Endometrium / Q000473:pathology* / Q000502:physiology*; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000502:physiology*; D018874:Spheroids, Cellular / Q000166:cytology / Q000502:physiology*; D014162:Transfection; D014327:Trophoblasts / Q000166:cytology / Q000502:physiology*
chemical_list: D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; D011257:Pregnancy Proteins