title: Lack of prognostic significance of angiogenesis in canine melanocytic tumours.
abstract: The prognostic significance of angiogenesis in some canine tumours has been investigated, but little is known about its relevance in canine melanocytic tumours (MTs). The aim of this study was to evaluate the prognostic significance of angiogenesis in canine MTs. A total of 36 cutaneous melanocytomas (benign MTs), 40 cutaneous melanomas (malignant MTs) and 43 oral melanomas were studied. Survival data were available for a subset of 59 cases. Microvessel density (MVD) and endothelial area (EA) were determined by immunolabelling using an antibody specific for von Willebrand factor (vWF). Mean MVD (expressed as the number of microvessels per mm(2)) was 129 ± 14 in melanocytomas, 191 ± 16 in cutaneous melanomas and 208 ± 16 in oral melanomas. Mean EA (expressed as the percentage of the total area) was 1.5 ± 0.14 in melanocytomas, 2.6 ± 0.2 in cutaneous melanomas and 2.4 ± 0.3 in oral melanomas. The differences in MVD and EA between melanocytomas and melanomas were significant (P = 0.001 and P = 0.003, respectively). MVD and EA were significantly correlated between cutaneous and oral MTs (r = 0.54; P <0.001 and r = 0.63; P <0.001, respectively). MVD and EA were not related to survival in cutaneous and oral MTs. In conclusion, tumour vascularization was higher in melanomas than in melanocytomas, but it seemed to have no prognostic significance in these tumours.
authors.affiliation: Laboratorio de Patología Especial, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina. mccuitino@fcv.unlp.edu.ar
authors.forename: M C
authors.initials: MC
authors.lastname: Cuitiño
authors.forename: A R
authors.initials: AR
authors.lastname: Massone
authors.forename: J R
authors.initials: JR
authors.lastname: Idiart
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D004283:Dog Diseases / Q000175:diagnosis* / Q000378:metabolism / Q000401:mortality; D004285:Dogs; D008544:Melanocytes / Q000473:pathology; D008545:Melanoma / Q000098:blood supply / Q000175:diagnosis / Q000401:mortality / Q000662:veterinary*; D055806:Microvessels / Q000473:pathology; D009062:Mouth Neoplasms / Q000098:blood supply / Q000175:diagnosis / Q000401:mortality / Q000662:veterinary*; D009389:Neovascularization, Pathologic / Q000175:diagnosis / Q000662:veterinary*; D011379:Prognosis; D012878:Skin Neoplasms / Q000098:blood supply / Q000175:diagnosis / Q000401:mortality / Q000662:veterinary*; D015996:Survival Rate