title: [Functions of plant phosphoenolpyruvate carboxylase and its applications for genetic engineering].
abstract: Phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) is an important ubiquitous cytosol enzyme that fixes HCO3 together with phosphoenolpyruvate (PEP) and yields oxaloacetate that can be converted to intermediates of the citric acid cycle. In plant cells, PEPC participates in CO2 assimilation and other important metabolic pathways, and it has broad functions in different plant tissues. PEPC is also involved in the regulation of storage product synthesis and metabolism in seeds, such as affecting the metabolic fluxes from sugars/starch towards the synthesis of fatty acids or amino acids and proteins. In this review, we introduced the progress in classification, structure and regulation of PEPC in plant tissues. We discussed the potential applications of plant PEPCs in genetic engineering. The researches in functions and regulation mechanism of plant PEPCs will provide beneficial approaches to applications of plant PEPCs in high-yield crops breeding, energy crop and microbe genetic engineering.
authors.affiliation: Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Shaowei
authors.initials: S
authors.lastname: Wei
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
mesh_terms: D001639:Bicarbonates / Q000737:chemistry; D005818:Genetic Engineering; D062907:Oxaloacetic Acid / Q000737:chemistry; D010728:Phosphoenolpyruvate / Q000737:chemistry*; D010730:Phosphoenolpyruvate Carboxylase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*
chemical_list: D001639:Bicarbonates; D062907:Oxaloacetic Acid; D010728:Phosphoenolpyruvate; D010730:Phosphoenolpyruvate Carboxylase