title: [Expression of GC-C mRNA in peripheral blood mononuclear cells and its clinical roles in determining state of gastric cancer].
abstract: OBJECTIVE
  To detect the expression of GC-C mRNA in peripheral blood of gastric carcinoma patients and determine its potential candidacy as a molecular biological marker for predicting the micrometastasis and determining the status of gastric cancer.
  
  
  METHODS
  GC-C mRNA from peripheral blood samples of gastric carcinoma (n = 60), dysplastic (n = 21), intestinal metaplasia (n = 15) and healthy cases (n = 20) from November 2009 to August 2010 at Affiliated Hospital of Nantong University were assessed by real-time fluorescent quantitative PCR (RFQ-PCR).
  
  
  RESULTS
  The expressions of GC-C mRNA in peripheral blood from patients with dysplasia, intestinal metaplasia and healthy controls were absent or very low. And a high level of GC-C mRNA was detected in the patients with gastric carcinoma than those with dysplastic and intestinal metaplasia, and the positive rate were 48.3% (29/60), 9.5% (2/21), 20.0% (3/15), respectively (all P < 0.05). The levels of GC-C mRNA were significantly correlated with Lauren typing, clinical stage, tumor differentiation degree, depth of invasion and lymph node metastasis (all P < 0.05). The GC-C mRNA expressions were positively correlated in peripheral blood and gastric carcinomas tissues (r = 0.4009, P = 0.0015).
  
  
  CONCLUSIONS
  The over-expression of GC-C mRNA is found in peripheral blood from gastric carcinoma patients. Due to its close correlation with clinical stage and lymph node metastasis, it may become a potential prognostic marker of gastric carcinoma.
authors.affiliation: Department of Gastroenterology, Nantong University, Nantong, China.
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Zhang
authors.forename: Shi-min
authors.initials: SM
authors.lastname: Xue
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Hai
authors.initials: H
authors.lastname: Meng
authors.forename: Hui-jun
authors.initials: HJ
authors.lastname: Zhu
authors.forename: Zhen-biao
authors.initials: ZB
authors.lastname: Mao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000097:blood / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D006162:Guanylate Cyclase / Q000235:genetics*; D006801:Humans; D007422:Intestines / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008679:Metaplasia; D008875:Middle Aged; D061206:Neoplasm Micrometastasis; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D013274:Stomach Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D006162:Guanylate Cyclase