title: Preparation of human scFv antibody against nasopharyngeal carcinoma and identification of its specificity.
abstract: Abstract    Conclusion: The selected scFv antibody could specifically recognize and target nasopharyngeal carcinoma (NPC), and could be applied to clinical diagnosis and therapy.
  
  
  OBJECTIVE
  The aim was to construct and screen fully human anti-NPC single chain Fv fusion phage libraries, and to identify the specificity of the scFv antibody.
  
  
  METHODS
  Peripheral blood mononuclear cells of patients with NPC were immunized in vitro by NPC cells and transformed by Epstein-Barr virus. The total RNAwas used to construct the scFv libraries. By means of ELISA and immunochemistry, the positively bound scFv was selected and identified. The positive scFv was fused to EGFP, and was then expressed in E. coli strain BL21 (DE3) and purified. Furthermore, we observed the binding bioactivity.
  
  
  RESULTS
  The fusion protein has the biological activity of binding the NPC cells and emitting green fluorescence. In targeting experiments in vivo, the results showed that the fusion protein can successfully target the NPC.
authors.affiliation: Cancer Research Institute, Central South University, Key Laboratory Of Carcinogenesis and Cancer Invasion, Ministry of Education and Health, Changsha, China.
authors.forename: Yan-Dong
authors.initials: YD
authors.lastname: Li
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Wang
authors.forename: Ping-Li
authors.initials: PL
authors.lastname: Xie
authors.forename: Feng-Jie
authors.initials: FJ
authors.lastname: Guo
authors.forename: Cai-Ping
authors.initials: CP
authors.lastname: Ren
authors.forename: Guan-Cheng
authors.initials: GC
authors.lastname: Li
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm / Q000235:genetics / Q000276:immunology*; D002277:Carcinoma; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis / Q000188:drug therapy / Q000276:immunology*; D009374:Neoplasms, Experimental; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000276:immunology* / Q000627:therapeutic use
chemical_list: D000912:Antibodies, Neoplasm; D057127:Single-Chain Antibodies