title: Identification of BoLA-DRB3.2 alleles in Korean native cattle (Hanwoo) and Holstein populations using a next generation sequencer.
abstract: Bovine leucocyte antigen (encoded by BoLA) has been widely studied to identify the association with many traits related to immunity. Exon2 of BoLA-DRB3 is extremely polymorphic, and more than 100 alleles have been identified. We investigated polymorphisms of BoLA-DRB3.2 in Korean native cattle and Holstein populations using a next generation sequencer of the GS-FLX Titanium system. We found 38 alleles including 11 new alleles (BoLA-DRB3*1303, *4702, *7101, *7501, *7201, *7301, *7601, *1104, *7701, *7401 and *50021) in Hanwoo, and nine alleles including one new allele (BoLA-DRB3*7601) in Holstein. The 454 sequencing method is a promising alternative technology for high throughput genotyping of BoLA-DRB3.2 because of its technical advantages that allow it to overcome the disadvantages of sequence-based typing methods.
authors.affiliation: Laboratory of Bioinformatics and Population Genetics, Department of Agricultural Biotechnology, Seoul National University, Republic of Korea.
authors.forename: Bo-Young
authors.initials: BY
authors.lastname: Lee
authors.forename: Tai-Young
authors.initials: TY
authors.lastname: Hur
authors.forename: Young-Hun
authors.initials: YH
authors.lastname: Jung
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics*; D005091:Exons; D005787:Gene Frequency / Q000235:genetics*; D005828:Genetics, Population; D005838:Genotype; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D011110:Polymorphism, Genetic; D056910:Republic of Korea; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: C423282:BoLA-DRB3 antigen; D000949:Histocompatibility Antigens Class II