title: Effect of caries preventive products on the growth of bacterial biofilm on titanium surface.
abstract: Fluorides may affect the oxide layer on titanium surface. Caries preventive mouthwashes or gels contain fluorides and are applied at low pH. The aim of the present work was to study whether various concentrations of fluoride at acidic pH cause changes in the surface structure on the polished region of Ti implants, and alter the adherence and colonization of bacteria. Commercially pure Ti grade 4 discs with a polished surface were treated with a mouthwash containing 0.025% fluoride, a gel containing 1.25% fluoride or a 1% aqueous solution of NaF (pH 4.5). The change of surface roughness of the samples and the colonization of Porphyromonas gingivalis strains were studied by scanning electron microscopy after 5 days of anaerobic incubation. The quantity of the bacterial protein was determined by protein assay analysis. Agents with high fluoride concentration at acidic pH increased the roughness of the Ti surface. A slight increase in the amount of bacteria was found on the surfaces treated with 1% NaF and gel in comparison with the control surface. This study suggested that a high fluoride concentration at acidic pH may hinder the development of a healthy transgingival epithelial junction on Ti implants, due to bacterial colonization.
authors.affiliation: Department of Prosthodontics and Oral Biology, University of Szeged, Szeged Hungary. stajeranette@gmail.com
authors.forename: Anette
authors.initials: A
authors.lastname: Stájer
authors.forename: Edit
authors.initials: E
authors.lastname: Urbán
authors.forename: István Kovács
authors.initials: IK
authors.lastname: Pelsõczi
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Mihalik
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Rakonczay
authors.forename: Katalin
authors.initials: K
authors.lastname: Nagy
authors.forename: Kinga
authors.initials: K
authors.lastname: Turzó
authors.forename: Márta
authors.initials: M
authors.lastname: Radnai
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D018441:Biofilms / Q000187:drug effects*; D003731:Dental Caries / Q000517:prevention & control*; D015921:Dental Implants / Q000382:microbiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D009067:Mouthwashes / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000502:physiology; D012969:Sodium Fluoride / Q000494:pharmacology; D013499:Surface Properties; D014025:Titanium
chemical_list: D001426:Bacterial Proteins; D015921:Dental Implants; D009067:Mouthwashes; D012969:Sodium Fluoride; D014025:Titanium