title: Development and application of a TaqMan real-time PCR assay for rapid detection of Magnaporthe poae.
abstract: In North America, one of the most important root diseases of Poa and Festuca turf is summer patch, caused by Magnaporthe poae. Detection and identification of M. poae in infected roots by conventional culture-based methods is difficult and time consuming, typically taking 3 wk or longer to accomplish. In this study, a culture-independent, TaqMan real-time PCR assay was developed for the detection of M. poae from the roots of fungicide treated and non-treated Kentucky bluegrass (Poa pratensis) turf. The assay was validated with the target pathogen, closely related fungal species and a number of other microorganisms that inhabit the same host and soil environment. This assay was more sensitive (could detect as little as 3.88 pg genomic DNA of M. poae), rapid and accurate compared to direct microscopic observation and isolation on a selective medium. The real-time PCR detection results corresponded closely to visual assessments of disease severity in the field. Utilization of this assay in diagnostic laboratories will enable turfgrass managers to more quickly and effectively detect and potentially reduce fungicide usage through early and accurate identification of the pathogen.
authors.affiliation: Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, China
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhao
authors.forename: Bruce B
authors.initials: BB
authors.lastname: Clarke
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
authors.forename: Lisa
authors.initials: L
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
mesh_terms: D004271:DNA, Fungal / Q000235:genetics*; D020082:Magnaporthe / Q000235:genetics* / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D031785:Poa / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004271:DNA, Fungal