title: DL-3-n-Butylphthalide protects rat bone marrow stem cells against hydrogen peroxide-induced cell death through antioxidation and activation of PI3K-Akt pathway.
abstract: Bone marrow stem cells (BMSCs) have been one of the most important cell sources for cell replacement therapy (CRT) in cerebral infarction. However, long-lasting oxidative stress during stroke, which plays an important role in neuron damage, deteriorates the microenvironment for cell survival, differentiation and removal. Thus the outcome of CRT in ischemic diseases was poor. DL-3-n-Butylphthalide (NBP) has protective effects on ischemic brain tissue through multiple mechanisms and has been used for stroke treatment in China for several years. In this study, hydrogen peroxide (H(2)O(2)) was used to induce oxidative stress injury to rat bone marrow stem cells (rBMSCs), imitating the microenvironment surrounding transplanted cells in the ischemic brain in vitro. The protective effects of NBP on rBMSCs against apoptosis induced by oxidative stress were investigated. Our results indicated that NBP could protect rBMSCs against apoptosis due to antioxidative properties and modulation of PI3K/Akt pathway. NBP could be used in combination with BMSCs for the treatment of cerebral infarction by improving the oxidative stress microenvironments and cell survival, however, further studies remain warranted.
authors.affiliation: Department of Neurology, Huaian No. 1 People's Hospital Affiliated to Nanjing Medical University, No.6 West Beijing Road, Huaian, 223000, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Meijiang
authors.initials: M
authors.lastname: Feng
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Tian
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Lu
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xianjin
authors.initials: X
authors.lastname: Ke
authors.forename: Susu
authors.initials: S
authors.lastname: Huang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Cao
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Ding
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001572:Benzofurans / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity; D016877:Oxidants / Q000633:toxicity; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000975:Antioxidants; D001572:Benzofurans; D016877:Oxidants; C027125:3-n-butylphthalide; D006861:Hydrogen Peroxide; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt