title: Th17 cell-derived IL-17 is dispensable for B cell antibody production.
abstract: IL-17, which is preferentially produced by Th17 cells, is important for host defense against pathogens and is also involved in the development of autoimmune and allergic disorders. Antibody (Ab) production was shown to be impaired in IL-17-deficient mice, suggesting that IL-17 may promote B cell activation and direct secretion of Ab. However, the precise role of IL-17 in Ab production by B cells remains unclear. In the present study, we found constitutive expression of IL-17R in murine splenic B cells. Nevertheless, IL-17, IL-17F or IL-25 alone could not induce Ab production by B cells even in the presence of agonistic anti-CD40 Ab. IL-17 also could not affect IFN-γ-, IL-4- or TGF-β1-mediated Ig class-switching. Furthermore, in co-cultures of B cells and IL-17(-/-) CD4(+) T cells or IL-17(-/-) Th17 cells, IL-17 deficiency did not influence Ab production by B cells in vitro, suggesting that Th17 cell-derived IL-17 was not required for B cell Ab production through T cell-B cell interaction in vitro. Thus, in vivo, IL-17 may be indirectly involved in Ab production by enhancing production of B cell activator(s) by other immune cells.
authors.affiliation: Department of Medical Genomics, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8561, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Shibui
authors.forename: Eri
authors.initials: E
authors.lastname: Shimura
authors.forename: Aya
authors.initials: A
authors.lastname: Nambu
authors.forename: Sachiko
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Warren J
authors.initials: WJ
authors.lastname: Leonard
authors.forename: Ko
authors.initials: K
authors.lastname: Okumura
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Katsuko
authors.initials: K
authors.lastname: Sudo
authors.forename: Susumu
authors.initials: S
authors.lastname: Nakae
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D017578:Immunoglobulin Class Switching / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D020381:Interleukin-17 / Q000172:deficiency / Q000276:immunology* / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053722:Receptors, Interleukin-17 / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D058504:Th17 Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D000098546:Interleukin-21
chemical_list: D020381:Interleukin-17; D007378:Interleukins; D012333:RNA, Messenger; D053722:Receptors, Interleukin-17; D053773:Transforming Growth Factor beta1; D015847:Interleukin-4; D007371:Interferon-gamma; D000098546:Interleukin-21