title: Strategy for rapid identification and antibiotic susceptibility testing of gram-negative bacteria directly recovered from positive blood cultures using the Bruker MALDI Biotyper and the BD Phoenix system.
abstract: Decreasing the time to species identification and antibiotic susceptibility determination of strains recovered from patients with bacteremia significantly decreases morbidity and mortality. Herein, we validated a method to identify Gram-negative bacteria directly from positive blood culture medium using the Bruker MALDI Biotyper and to rapidly perform susceptibility testing using the BD Phoenix.
authors.affiliation: Department of Pathology and Genomic Medicine, The Methodist Hospital, Houston, TX, USA.
authors.forename: Jana L
authors.initials: JL
authors.lastname: Wimmer
authors.forename: S Wesley
authors.initials: SW
authors.lastname: Long
authors.forename: Patricia
authors.initials: P
authors.lastname: Cernoch
authors.forename: Geoffrey A
authors.initials: GA
authors.lastname: Land
authors.forename: James R
authors.initials: JR
authors.lastname: Davis
authors.forename: James M
authors.initials: JM
authors.lastname: Musser
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Olsen
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D015373:Bacterial Typing Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents