title: [Preliminary study on inhibition of the hair follicle development by siRNA targeting Wnt10b in the cultured rat embryonic skin].
abstract: OBJECTIVE
  To investigate whether the suppression of Wnt10b by siRNA could prevent the development of hair follicle in the cultured rat embryonic skin.
  
  
  METHODS
  siRNA-Wnt10b was synthesized by chemosynthesis method. The dorsal skin of SD rat at embryos were cultured in DMEM in the presence of different percentage of interfering RNA targeting Wnt10b. Wnt10b/beta-catenin expression was analyzed by real-time PCR everyday and by Western blot on the third day. The cultured embryonic skin underwent paraffin embedding, section, HE staining on the third day,in which the number of de novo hair follicle was calculated and statistically analyzed.
  
  
  RESULTS
  Wnt10b gene in the cultured embryonic skin could be knocked down with the siRNA-based method. Beta-catenin mRNA was not greatly influenced by the downregulation of Wnt10b mRNA. The number of de novo hair follicle placode in cultured embryonic skin decreased, along with the downregulation of Wnt10b and beta-catenin proteins expression.
  
  
  CONCLUSIONS
  The downregulation of Wnt10b mRNA and protein by siRNA reduces the number of de novo hair follicle placode in the cultured rat embryonic skin. Wnt10b may control cytoplasm beta-catenin concentration at the protein level.
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital, Shantou University Medical College, Shantou 515041, China.
authors.forename: Ying-Chang
authors.initials: YC
authors.lastname: Ji
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Hu
authors.forename: Sen
authors.initials: S
authors.lastname: Wang
authors.forename: Chang-Min
authors.initials: CM
authors.lastname: Lin
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Gao
mesh_terms: D000818:Animals; D018859:Hair Follicle / Q000196:embryology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D051381:Rats; D012867:Skin / Q000196:embryology / Q000378:metabolism; D046509:Tissue Culture Techniques; D051153:Wnt Proteins / Q000235:genetics* / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051153:Wnt Proteins; C501228:Wnt10b protein, rat; D051176:beta Catenin