title: Circulating microRNAs as potential biomarkers for smoking-related interstitial fibrosis.
abstract: Numerous efforts have been made to indentify reliable and predictive biomarkers to detect the early signs of smoking-induced lung disease. Using 6-month cigarette smoking in mice, we have established smoking-related interstitial fibrosis (SRIF). Microarray analyses and cytokine/chemokine biomarker measurements were made to select circulating microRNAs (miRNAs) biomarkers. We have demonstrated that specific miRNAs species (miR-125b-5p, miR-128, miR-30e, and miR-20b) were significantly changed, both in the lung tissue and in plasma, and exhibited mainstream (MS) exposure duration-dependent pathological changes in the lung. These findings suggested a potential use of specific circulating miRNAs as sensitive and informative biomarkers for smoking-induced lung disease.
authors.affiliation: West China Hospital, Sichuan University, Chengdu, China. huangyuchuan1999@yahoo.com.cn
authors.forename: Yuchuan
authors.initials: Y
authors.lastname: Huang
authors.forename: Ya
authors.initials: Y
authors.lastname: Dai
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Changguo
authors.initials: C
authors.lastname: Wang
authors.forename: Dongliang
authors.initials: D
authors.lastname: Li
authors.forename: Jingqiu
authors.initials: J
authors.lastname: Cheng
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Lu
authors.forename: Kuoyan
authors.initials: K
authors.lastname: Ma
authors.forename: Lanlan
authors.initials: L
authors.lastname: Tan
authors.forename: Fang
authors.initials: F
authors.lastname: Xue
authors.forename: Bo
authors.initials: B
authors.lastname: Qin
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D018925:Chemokines / Q000097:blood; D016000:Cluster Analysis; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006801:Humans; D017563:Lung Diseases, Interstitial / Q000097:blood* / Q000209:etiology / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011658:Pulmonary Fibrosis / Q000097:blood* / Q000209:etiology / Q000235:genetics; D012907:Smoking / Q000009:adverse effects; D014028:Tobacco Smoke Pollution / Q000009:adverse effects
chemical_list: D015415:Biomarkers; D018925:Chemokines; D016207:Cytokines; D035683:MicroRNAs; C527468:Mirn125 microRNA, mouse; C550324:Mirn128 microRNA, mouse; C532375:Mirn20 microRNA, mouse; C539917:Mirn31 microRNA, mouse; D014028:Tobacco Smoke Pollution