title: Real-time quantitative visualization of 3D structural information.
abstract: We demonstrate a novel approach for the real time visualization and quantification of the 3D spatial frequencies in an image domain. Our approach is based on the spectral encoding of spatial frequency principle and permits the formation of an image as a color map in which spatially separated spectral wavelengths correspond to the dominant 3D spatial frequencies of the object. We demonstrate that our approach can visualize and analyze the dominant axial internal structure for each image point in real time and with nanoscale sensitivity to structural changes. Computer modeling and experimental results of instantaneous color visualization and quantification of 3D structures of a model system and biological samples are presented.
authors.affiliation: Biomedical Optical Imaging Laboratory (BOIL), Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15232, USA.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Alexandrov
authors.forename: Shikhar
authors.initials: S
authors.lastname: Uttam
authors.forename: Rajan K
authors.initials: RK
authors.lastname: Bista
authors.forename: Chengquan
authors.initials: C
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D002584:Cervix Uteri / Q000166:cytology; D003198:Computer Simulation; D003199:Computer Systems; D005260:Female; D005583:Fourier Analysis; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods* / Q000706:statistics & numerical data; D055620:Optical Phenomena; D002583:Uterine Cervical Neoplasms / Q000473:pathology; D014626:Vaginal Smears / Q000706:statistics & numerical data; D002578:Uterine Cervical Dysplasia / Q000473:pathology