title: A TEMPO-conjugated fluorescent probe for monitoring mitochondrial redox reactions.
abstract: We report a mitochondrial targeted redox probe (MitoRP) that comprises a nitroxide radical (TEMPO) moiety and coumarin 343. Using isolated mitochondria in the presence/absence of substrates and inhibitors of oxidative phosphorylation, we demonstrated that MitoRP is a useful probe to monitor the electron flow associated with complex I.
authors.affiliation: Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University (TWIns), Tokyo 162-8480, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Hirosawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Arai
authors.forename: Shinji
authors.initials: S
authors.lastname: Takeoka
mesh_terms: D003374:Coumarins / Q000737:chemistry; D003497:Cyclic N-Oxides / Q000737:chemistry*; D042967:Electron Transport Complex I / Q000037:antagonists & inhibitors / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation
chemical_list: D003374:Coumarins; D003497:Cyclic N-Oxides; D005456:Fluorescent Dyes; C552279:coumarin 343; D009243:NAD; D042967:Electron Transport Complex I; C003959:TEMPO