title: [Selection of culture media for the mass production of gamma delta T cells used in adoptive immunotherapy].
abstract: OBJECTIVE
  To select the optimal culture media for the mass production of gamma delta T cells used in adoptive immunotherapy.
  
  
  METHODS
  Three different culture media (RPMI-1640, AIM-V, and OpTmizer, with or without autologous serum) were used to culture gamma delta T cells. The survival rate, purity, proliferation efficiency, and biological functions of the expanded gamma delta T cells were examined and compared.
  
  
  RESULTS
  The survival rate of gamma delta T cells expanded in RPMI-1640 decreased over culture time. The purities of gamma delta T cells cultured in AIM-V or OpTmizer with or without serum were higher than those cultured in RPMI-1640. After two weeks of culture in the absence of serum, the purity and proliferation efficiency of gamma delta T cells cultured in OpTmizer were significantly higher than those cultured in RPMI-1640 (P < 0.05) and AIM-V (P < 0.05). gamma delta T cells in different culture media had similar CD107a expression and tumor necrosis factor-alpha production (P > 0.05). However, cells expanded in RPMI-1640 exhibited significantly weaker cytotoxicity against Daudi lymphoma cells than those expanded in OpTmizer (P < 0.05) and AIM-V (P < 0.05).
  
  
  CONCLUSIONS
  Due to low serum-dependence, high proliferation efficiency, and favorable biology function of expanded cells, OpTmizer is the most suitable medium for the mass production of gamma delta T cells used in adoptive immunotherapy.
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhou
authors.forename: Ning
authors.initials: N
authors.lastname: Kang
authors.forename: Lian-Xian
authors.initials: LX
authors.lastname: Cui
authors.forename: Wei
authors.initials: W
authors.lastname: He
mesh_terms: D018929:Cell Culture Techniques; D003470:Culture Media; D006801:Humans; D016219:Immunotherapy, Adoptive; D013601:T-Lymphocytes / Q000166:cytology*
chemical_list: D003470:Culture Media