title: Chemical synthesis of intentionally misfolded homogeneous glycoprotein: a unique approach for the study of glycoprotein quality control.
abstract: Biosynthesis of glycoproteins in the endoplasmic reticulum employs a quality control system, which discriminates and excludes misfolded malfunctional glycoproteins from a correctly folded one. As chemical tools to study the glycoprotein quality control system, we systematically synthesized misfolded homogeneous glycoproteins bearing a high-mannose type oligosaccharide via oxidative misfolding of a chemically synthesized homogeneous glycopeptide. The endoplasmic reticulum folding sensor enzyme, UDP-glucose:glycoprotein glucosyltransferase (UGGT), recognizes a specific folding intermediate, which exhibits a molten globule-like hydrophobic nature.
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Izumi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Makimura
authors.forename: Simone
authors.initials: S
authors.lastname: Dedola
authors.forename: Akira
authors.initials: A
authors.lastname: Seko
authors.forename: Akiko
authors.initials: A
authors.lastname: Kanamori
authors.forename: Masafumi
authors.initials: M
authors.lastname: Sakono
authors.forename: Yukishige
authors.initials: Y
authors.lastname: Ito
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kajihara
mesh_terms: D000595:Amino Acid Sequence; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D005964:Glucosyltransferases / Q000378:metabolism*; D006020:Glycopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D006023:Glycoproteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008358:Mannose / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D010084:Oxidation-Reduction; D017510:Protein Folding; D013379:Substrate Specificity
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins; D009844:Oligosaccharides; D005964:Glucosyltransferases; C072423:mannosylglycoprotein 1,3-glucosyltransferase; D008358:Mannose