title: Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry.
abstract: Rapid, sensitive, and highly specific flow-cytometric assays were developed for the detection of the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) O groups in ground beef. The analytical sensitivity of the assays was 2 × 10(3) target cells in a bacterial mixture of 10(5) CFU/ml, and the limit of detection in ground beef was 1 to 10 CFU following 8 h of enrichment. The assays may be utilized for rapid detection of STEC O groups in meat.
authors.affiliation: Escherichia coli Reference Center, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania, USA.
authors.forename: Narasimha V
authors.initials: NV
authors.lastname: Hegde
authors.forename: Bhushan M
authors.initials: BM
authors.lastname: Jayarao
authors.forename: Chitrita
authors.initials: C
authors.lastname: DebRoy
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002417:Cattle; D005434:Flow Cytometry / Q000379:methods*; D008460:Meat / Q000382:microbiology*; D012680:Sensitivity and Specificity; D054323:Shiga-Toxigenic Escherichia coli / Q000302:isolation & purification*; D013997:Time Factors