title: Assessment of anti-angiogenic and anti-tumoral potentials of Origanum onites L. essential oil.
abstract: Medicinal plants and culinary herbs with anti-angiogenic and little toxicity properties have gained importance. Non-toxic anti-angiogenic phytochemicals are useful in combating cancer by preventing the formation of new blood vessels to support the tumor growth. We have investigated the essential oil of Origanum onites L. (OOEO), for a possible anti-angiogenic activity. OOEO was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). The anti-proliferative activities (by MTT assay, 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H-tetrazolium bromide), anti-angiogenic activities (by tube formation assay), cell migration inhibiting capability (migration assay) and apoptotic potential (DAPI staining) of OOEO were evaluated on rat adipose tissue endothelial cells (RATECs) and 5RP7 (c-H-ras transformed rat embryonic fibroblasts) cells. Our results revealed that OOEO could markedly inhibit cell viability and induced apoptosis of 5RP7 cells and also could block in vitro tube formation and migration of RATEC. These results imply that OOEO having anti-angiogenic activity might be useful in preventing angiogenesis-related diseases and in combating cancer.
authors.affiliation: Anadolu University, Faculty of Sciences, Department of Biology, 26470-Eskişehir, Turkey. beklemb@gmail.com
authors.forename: Rakibe Beklem
authors.initials: RB
authors.lastname: Bostancıoğlu
authors.forename: Mine
authors.initials: M
authors.lastname: Kürkçüoğlu
authors.forename: Kemal Hüsnü Can
authors.initials: KH
authors.lastname: Başer
authors.forename: Ayşe Tansu
authors.initials: AT
authors.lastname: Koparal
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects; D020533:Angiogenesis Inhibitors; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D017209:Apoptosis / Q000187:drug effects; D002196:Capillaries / Q000187:drug effects / Q000254:growth & development; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002849:Chromatography, Gas; D042783:Endothelial Cells / Q000187:drug effects; D005347:Fibroblasts; D005456:Fluorescent Dyes; D008401:Gas Chromatography-Mass Spectrometry; D007211:Indoles; D009822:Oils, Volatile / Q000494:pharmacology*; D031341:Origanum / Q000737:chemistry*; D051381:Rats
chemical_list: D020533:Angiogenesis Inhibitors; D000972:Antineoplastic Agents, Phytogenic; D005456:Fluorescent Dyes; D007211:Indoles; D009822:Oils, Volatile; C029292:origanum oil; C007293:DAPI