title: [Roles of axonal transport affected by K141N mutant HSP22 in the pathogenesis of CMT2L].
abstract: OBJECTIVE
  To compare the axonal transport of wild-type (WT) and K141N mutant HSP22 in transfected primary cultured cortical neurons.
  
  
  METHODS
  The plasmid (pCAGGS-HA-wtHSP22 or pCAGGS-HA-K141NHSP22) with WT or K141N mutant HSP22 gene and a GFP-expressing plasmid (pEGFP-N1) were co-transfected respectively into primary cultured cortical neurons. The axonal transport of WT and K141N mutant HSP22 was observed. And the distance traveled by WT and K141N mutant HSP22 was analyzed.
  
  
  RESULTS
  The WT HSP22 was transported within axons and uniformly present throughout the entire length of axons. K141N mutant HSP22 failed to be transported to the same extent and was present only in cell body and proximal portion of axons. Analysis of distance traveled revealed that WT HSP22 traveled significantly further than the K141N mutant HSP22.
  
  
  CONCLUSIONS
  The axonal transport of K141N mutant HSP22 may play an important role in the pathogenesis of CMT2L.
authors.affiliation: Department of Geriatrics, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Fu-Feng
authors.initials: FF
authors.lastname: Zhang
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Lu
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Zhou
authors.forename: Lu
authors.initials: L
authors.lastname: Shen
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Xin-Xiang
authors.initials: XX
authors.lastname: Yan
authors.forename: Bei-Sha
authors.initials: BS
authors.lastname: Tang
mesh_terms: D000818:Animals; D001370:Axonal Transport / Q000235:genetics*; D002478:Cells, Cultured; D002607:Charcot-Marie-Tooth Disease / Q000235:genetics / Q000473:pathology*; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D009154:Mutation; D009474:Neurons; D014162:Transfection
chemical_list: D006360:Heat-Shock Proteins