title: Supramolecular displacement-mediated activation of a silent fluorescence probe for label-free ligand screening.
abstract: We report a new approach for the rapid screening of analyte binding affinities for a target protein. We demonstrate that a molecular probe, with a pro-fluorophore substrate and ligand moieties, can be hindered from enzymatic access when bound to the target protein. When analytes displace the probe from the protein's binding pocket, a fluorescence profile is generated. This profile is used to discriminate analytes based on their relative binding affinities.
authors.affiliation: Department of Chemistry, University of Massachusetts at Amherst, Amherst, Massachusetts 01003, USA.
authors.forename: Diego Amado
authors.initials: DA
authors.lastname: Torres
authors.forename: Malar A
authors.initials: MA
authors.lastname: Azagarsamy
authors.forename: S
authors.initials: S
authors.lastname: Thayumanavan
mesh_terms: D024401:Carbonic Anhydrase I / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D011485:Protein Binding
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D024401:Carbonic Anhydrase I