title: Macrophage immunomodulatory activity of a purified polysaccharide isolated from Ganoderma atrum.
abstract: The objective of this study was to evaluate the immunomodulatory effects of the purified Ganoderma atrum polysaccharide (PSG-1) on murine macrophage cell line RAW264.7. Phagocytotic assay by fluorescein isothiocyanate-dextran internalization showed that PSG-1 stimulated the phagocytosis of macrophages. G. atrum polysaccharide increased the production of NO, and the level of mRNA expression of inducible nitric oxide synthase in a dose-response manner. G. atrum polysaccharide also dose-dependently induced the release of TNF-α and interleukin-1β. Generation of reactive oxygen species was promoted by PSG-1, as determined by flow cytometry. Moreover, PSG-1 induced nuclear factor-κB activation by elevation of p65 nuclear translocation, suggesting that PSG-1 probably stimulated macrophage activities by activating the nuclear factor-κB pathway.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yu
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Nie
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Li
authors.forename: Wen-Ya
authors.initials: WY
authors.lastname: Zheng
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Yin
authors.forename: De-Ming
authors.initials: DM
authors.lastname: Gong
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Xie
mesh_terms: D000818:Animals; D002460:Cell Line; D062610:Fungal Polysaccharides / Q000494:pharmacology*; D032902:Ganoderma / Q000737:chemistry*; D053583:Interleukin-1beta / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D062610:Fungal Polysaccharides; D053583:Interleukin-1beta; D017382:Reactive Oxygen Species; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse