title: Visualizing metal ions in cells: an overview of analytical techniques, approaches, and probes.
abstract: Quantifying the amount and defining the location of metal ions in cells and organisms are critical steps in understanding metal homeostasis and how dyshomeostasis causes or is a consequence of disease. A number of recent advances have been made in the development and application of analytical methods to visualize metal ions in biological specimens. Here, we briefly summarize these advances before focusing in more depth on probes for examining transition metals in living cells with high spatial and temporal resolution using fluorescence microscopy. This article is part of a Special Issue entitled: Cell Biology of Metals.
authors.affiliation: UCB 596, Department of Chemistry, BioFrontiers Institute, University of Colorado, Boulder, CO 80309, United States.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Dean
authors.forename: Yan
authors.initials: Y
authors.lastname: Qin
authors.forename: Amy E
authors.initials: AE
authors.lastname: Palmer
mesh_terms: D000818:Animals; D002413:Cations, Divalent / Q000032:analysis* / Q000378:metabolism; D002414:Cations, Monovalent / Q000032:analysis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D008670:Metals / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000737:chemistry*; D051381:Rats
chemical_list: D002413:Cations, Divalent; D002414:Cations, Monovalent; D005456:Fluorescent Dyes; D008670:Metals; D015335:Molecular Probes