title: Innate and adaptive immune responses during acute M. tuberculosis infection in adult household contacts in Kampala, Uganda.
abstract: Contacts of active pulmonary tuberculosis (TB) patients are at risk for Mycobacterium tuberculosis (MTB) infection. Because most infections are controlled, studies during MTB infection provide insight into protective immunity. We compared immune responses of adult household contacts that did and did not convert the tuberculin skin test (TST). Innate and adaptive immune responses were measured by whole blood assay. Responses of TST converters (TSTC) were compared with persistently TST negative contacts (PTST-) and contacts who were TST+ at baseline (TST+). TLR-2, TLR-4, and IFN-γR responses to IFN-γ did not differ between the groups, nor did γδ T cell responses. T cell responses to MTB antigens differed markedly among TSTC, PTST-, and TST+ contacts. Thus, no differences in innate responses were found among the three household contact groups. However, adaptive T cell responses to MTB antigens did differ before and during MTB infection among PTST-, TSTC, and TST+ contacts.
authors.affiliation: Department of Medicine, MetroHealth Medical Center, Cleveland, Ohio 44109, USA. csmahan@gmail.com
authors.forename: C Scott
authors.initials: CS
authors.lastname: Mahan
authors.forename: Sarah
authors.initials: S
authors.lastname: Zalwango
authors.forename: Bonnie A
authors.initials: BA
authors.lastname: Thiel
authors.forename: LaShaunda L
authors.initials: LL
authors.lastname: Malone
authors.forename: Keith A
authors.initials: KA
authors.lastname: Chervenak
authors.forename: Joy
authors.initials: J
authors.lastname: Baseke
authors.forename: Dennis
authors.initials: D
authors.lastname: Dobbs
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Stein
authors.forename: Harriet
authors.initials: H
authors.lastname: Mayanja
authors.forename: Moses
authors.initials: M
authors.lastname: Joloba
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Whalen
authors.forename: W Henry
authors.initials: WH
authors.lastname: Boom
mesh_terms: D000208:Acute Disease; D056704:Adaptive Immunity; D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism; D002648:Child; D005191:Family Characteristics; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D008297:Male; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity; D017471:Receptors, Interferon / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000276:immunology / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000276:immunology / Q000378:metabolism; D014374:Tuberculin Test / Q000379:methods; D014376:Tuberculosis / Q000276:immunology* / Q000473:pathology; D014454:Uganda / Q000453:epidemiology; D055815:Young Adult; D000096743:Interferon gamma Receptor
chemical_list: D000942:Antigens, Bacterial; D017471:Receptors, Interferon; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D000096743:Interferon gamma Receptor; C495342:TLR2 protein, human; C495345:TLR4 protein, human; C072681:Mycobacterium tuberculosis antigens