title: Mass spectrometric elucidation of triacylglycerol content of Brevoortia tyrannus (menhaden) oil using non-aqueous reversed-phase liquid chromatography under ultra high pressure conditions.
abstract: A non-aqueous reversed phase high performance liquid chromatography method was developed, and optimized for triacylglycerol analysis in a Brevoortia tyrannus (menhaden) oil sample. Four columns were serially coupled to tackle such a task, for a total length of 60 cm of shell-packed stationary phase, and operated under ultra high pressure conditions. As detection, positive-ion atmospheric pressure chemical ionization mass spectrometry was used to attain identification of the analyzed sample components. A number of 137 triacylglycerols containing up to 19 fatty acids, with 14-22 carbon atom alkyl chain length and 0-6 double bonds, were positively identified in the complex lipidic sample. This is the first work that reports an extensive characterization of the triacylglycerol fraction of menhaden oil.
authors.affiliation: Dipartimento Farmaco-chimico, Università di Messina, viale Annunziata, 98168 Messina, Italy. pdugo@unime.it
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.forename: Marco
authors.initials: M
authors.lastname: Beccaria
authors.forename: Nermeen
authors.initials: N
authors.lastname: Fawzy
authors.forename: Paola
authors.initials: P
authors.lastname: Donato
authors.forename: Francesco
authors.initials: F
authors.lastname: Cacciola
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005395:Fish Oils / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D014280:Triglycerides / Q000032:analysis*
chemical_list: D005395:Fish Oils; D014280:Triglycerides; C033266:Menhaden oil