title: A novel USP9Y polymorphism allowing a rapid and unambiguous classification of Bos taurus Y chromosomes into haplogroups.
abstract: A new sequence-tagged site (STS) was identified within intron 26 of the bovine USP9Y gene, showing an 81-base pair insertion (g.76439_76440ins81 in sequence with GenBank accession FJ195366) able to distinguish Y2 and Y3 Bos Y haplogroups from Y1. Moreover, four Y3-specific sequence variants allow a distinction from haplogroup Y2. The typing of a Bison bison Y chromosome indicates that the ancestral allele for the USP9Y 81-bp insertion is the short Y1 version. The results from typing the new STS in 1230 cattle Y chromosomes are fully consistent with their classification through standard methods. Thanks to the newly identified STS, it is now possible to assign cattle Y chromosomes to the currently known haplogroups using a single marker.
authors.affiliation: Dipartimento di Genetica e Microbiologia A. Buzzati-Traverso, Università di Pavia, 27100, Pavia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Bonfiglio
authors.forename: A
authors.initials: A
authors.lastname: De Gaetano
authors.forename: K
authors.initials: K
authors.lastname: Tesfaye
authors.forename: V
authors.initials: V
authors.lastname: Grugni
authors.forename: O
authors.initials: O
authors.lastname: Semino
authors.forename: L
authors.initials: L
authors.lastname: Ferretti
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000145:classification*; D019143:Evolution, Molecular; D005819:Genetic Markers; D006239:Haplotypes; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D016324:Sequence Tagged Sites; D013869:Thiolester Hydrolases / Q000235:genetics*; D014998:Y Chromosome / Q000145:classification*
chemical_list: D005819:Genetic Markers; D013869:Thiolester Hydrolases