title: [Community structure of ammonia-oxidizing prokaryotes at the dry-up lake in Huitengxile grassland].
abstract: OBJECTIVE
  To investigate the structure of ammonia-oxidation microbial communities in the wetlands to dry-up process at 99 degraded lakes of the Huitengxile grassland in the Inner Mongolia Plateau.
  
  
  METHODS
  The microbial quantity of ammonia-oxidizing archaea (AOA) and ammonia oxidizing bacteria (AOB) were examined by most probable number-polymerase chain reaction (MPN-PCR). The clone libraries of amoA were constructed and phylogenetics were analyzed. With analysis of the soil properties, we evaluated the effects of wetlands degradation on ammonia-oxidation microbes communities.
  
  
  RESULTS
  In 75% of the samples, the quantity of AOB communities was higher than that of AOA; moreover, quantity of bacterial were up to 18.1-fold more abundant than Archaea's. The AOB microbial quantity was strongly correlated with NH4+-N content in the soil. Phylogenetic analyses of the amoA gene fragments showed that most AOB sequences from degraded wetlands were affiliated with Nitrosomonas-like species and a few close to Nitrosospira. All AOA sequences belonged to the kingdom Crenarchaeote.
  
  
  CONCLUSIONS
  Experimental results showed that quantity of ammonia-oxidation microbes increased but community diversity declined during wetlands degradation , and oxidation conditions and ammonium concentration in the soil might play important roles in the community structure of both the AOA and AOB.
authors.affiliation: College of Life Sciences, Inner Mongolia University, Huhhot 010021, China. huilihua-1984@163.com
authors.forename: Lihua
authors.initials: L
authors.lastname: Hui
authors.forename: Ji
authors.initials: J
authors.lastname: Zhao
authors.forename: Linhui
authors.initials: L
authors.lastname: Wu
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Shao
authors.forename: Jingyu
authors.initials: J
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000302:isolation & purification*; D001419:Bacteria / Q000302:isolation & purification*; D060106:Lakes; D010084:Oxidation-Reduction; D010802:Phylogeny; D012988:Soil Microbiology
chemical_list: D000641:Ammonia