title: Are the interactions between recombinant prion proteins and polymeric surfaces related to the hydrophilic/hydrophobic balance?
abstract: New non-fouling tubes are developed and their influence on the adhesion of neuroproteins is studied. Recombinant prion proteins are considered as a single component representative of hydrophobic proteins. Samples are stored for 24 h at 4 °C in tubes coated with two different coatings: poly(N-isopropylacrylamide) as a hydrophilic surface and a plasma-fluorinated coating as a hydrophobic one. The protein adhesion is monitored by ELISA tests, XPS and confocal microscopy. It appears that the highest recovery of recombinant prion protein in the liquid phase is obtained with the hydrophilic surface while the hydrophobic character of the storage tube induces an important amount of biological loss. However, the recovery is not complete even for tubes coated with poly(N-isopropylacrylamide).
authors.affiliation: LUNAM Université, UMR Université du Maine-CNRS n° 6283, Institut des Molécules et Matériaux du Mans-Département, Av. O. Messiaen, 72085 Le Mans, France.
authors.forename: Tjasa
authors.initials: T
authors.lastname: Vrlinic
authors.forename: Dominique
authors.initials: D
authors.lastname: Debarnot
authors.forename: Gilbert
authors.initials: G
authors.lastname: Legeay
authors.forename: Arnaud
authors.initials: A
authors.lastname: Coudreuse
authors.forename: Benaissa
authors.initials: B
authors.lastname: El Moualij
authors.forename: Willy
authors.initials: W
authors.lastname: Zorzi
authors.forename: Armand
authors.initials: A
authors.lastname: Perret-Liaudet
authors.forename: Isabelle
authors.initials: I
authors.lastname: Quadrio
authors.forename: Miran
authors.initials: M
authors.lastname: Mozetic
authors.forename: Fabienne
authors.initials: F
authors.lastname: Poncin-Epaillard
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D011108:Polymers / Q000737:chemistry*; D011328:Prions / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; D011328:Prions; D011994:Recombinant Proteins; C052970:poly-N-isopropylacrylamide