title: Comparison of Sebia Capillarys Flex capillary electrophoresis with the BioRad Variant II high pressure liquid chromatography in the evaluation of hemoglobinopathies.
abstract: BACKGROUND
  There are multiple biochemical screening techniques for assessing hemoglobinopathies. Here we compare a new instrument, the Sebia Capillarys Flex (capillary zone electrophoresis (CE)), with the BioRad Variant II (high pressure liquid chromatography (HPLC) in the evaluation of hemoglobinopathies.
  
  
  METHODS
  This was a retrospective study using 174 whole blood samples encompassing the 5 most common (Hb A, A2', S, C, and E) and 10 rare (Hb G(Philidelphia), D, H, Bart's, O(Arab), S/G(Philidelphia), Hasharoon, Q(India), N(Baltimore), and Malmo) hemoglobin variants. An additional 126 samples were used to establish a CE reference interval for Hb A2.
  
  
  RESULTS
  Hb A measurements agreed well between the 2 methods (bias=-0.06;r=0.999). The agreement of Hb F was also very good (bias=-0.17; r=0.994). When samples with the highest Hb F concentration were excluded, agreement was less precise (bias=-0.44; r=0.811). When no variant was present, the Hb A2 concentrations showed excellent agreement (bias=0.00; r=0.994). Positive bias for Hb A2 is seen when Hb C is present using CE. The Hb A2 reference interval using CE was <3.2%.
  
  
  CONCLUSIONS
  The Capillarys Flex is capable of identifying and quantifying hemoglobin species, consistent with existing HPLC methods.
authors.affiliation: The Permanente Medical Group Regional Laboratories, Kaiser Permanente Northern California, Berkeley, CA, United States. dina.n.greene@kp.org
authors.forename: Dina N
authors.initials: DN
authors.lastname: Greene
authors.forename: Amy L
authors.initials: AL
authors.lastname: Pyle
authors.forename: Judy S
authors.initials: JS
authors.lastname: Chang
authors.forename: Carolyn
authors.initials: C
authors.lastname: Hoke
authors.forename: Thomas
authors.initials: T
authors.lastname: Lorey
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005319:Fetal Hemoglobin / Q000032:analysis; D006443:Hemoglobin A2 / Q000032:analysis; D006444:Hemoglobin C / Q000032:analysis; D006446:Hemoglobin E / Q000032:analysis; D006451:Hemoglobin, Sickle / Q000032:analysis; D006453:Hemoglobinopathies / Q000175:diagnosis* / Q000235:genetics; D006454:Hemoglobins / Q000032:analysis / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D012016:Reference Values; D015203:Reproducibility of Results; D012189:Retrospective Studies
chemical_list: D006451:Hemoglobin, Sickle; D006454:Hemoglobins; D006444:Hemoglobin C; D006443:Hemoglobin A2; D006446:Hemoglobin E; D005319:Fetal Hemoglobin