title: β2AR antagonists and β2AR gene deletion both promote skin wound repair processes.
abstract: Skin wound healing is a complex process requiring the coordinated, temporal orchestration of numerous cell types and biological processes to regenerate damaged tissue. Previous work has demonstrated that a functional β-adrenergic receptor autocrine/paracrine network exists in skin, but the role of β2-adrenergic receptor (β2AR) in wound healing is unknown. A range of in vitro (single-cell migration, immunoblotting, ELISA, enzyme immunoassay), ex vivo (rat aortic ring assay), and in vivo (chick chorioallantoic membrane assay, zebrafish, murine wild-type, and β2AR knockout excisional skin wound models) models were used to demonstrate that blockade or loss of β2AR gene deletion promoted wound repair, a finding that is, to our knowledge, previously unreported. Compared with vehicle-only controls, β2AR antagonism increased angiogenesis, dermal fibroblast function, and re-epithelialization, but had no effect on wound inflammation in vivo. Skin wounds in β2AR knockout mice contracted and re-epithelialized faster in the first few days of wound repair in vivo. β2AR antagonism enhanced cell motility through distinct intracellular signalling mechanisms and increased vascular endothelial growth factor secretion from keratinocytes. β2AR antagonism promoted wound repair processes in the early stages of wound repair, revealing a possible new avenue for therapeutic intervention.
authors.affiliation: Deparment of Cell Physiology and Pharmacology, University of Leicester, Leicester, UK. cp161@le.ac.uk
authors.forename: Christine E
authors.initials: CE
authors.lastname: Pullar
authors.forename: Gabrielle S
authors.initials: GS
authors.lastname: Le Provost
authors.forename: Andrew P
authors.initials: AP
authors.lastname: O'Leary
authors.forename: Sian E
authors.initials: SE
authors.lastname: Evans
authors.forename: Brian S
authors.initials: BS
authors.lastname: Baier
authors.forename: R Rivkah
authors.initials: RR
authors.lastname: Isseroff
mesh_terms: D058672:Adrenergic beta-2 Receptor Antagonists / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000473:pathology; D002642:Chick Embryo; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005347:Fibroblasts / Q000378:metabolism; D017353:Gene Deletion; D007249:Inflammation; D015603:Keratinocytes / Q000166:cytology; D051379:Mice; D009389:Neovascularization, Pathologic; D051381:Rats; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics*; D012867:Skin / Q000473:pathology*; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis; D014945:Wound Healing; D015027:Zebrafish
chemical_list: D058672:Adrenergic beta-2 Receptor Antagonists; D018343:Receptors, Adrenergic, beta-2; D042461:Vascular Endothelial Growth Factor A