title: Rapid determination of methicillin resistance among Staphylococcus aureus clinical isolates by colorimetric methods.
abstract: In the present study, the effectiveness of a rapid and colorimetric nitrate reductase analysis (NRA) method and resazurin microplate assay (REMA) for rapid determination of methicillin resistance among Staphylococcus aureus was investigated. A total of 275 clinical isolates of S. aureus were included in the present study. Among these isolates, 151 had the mecA gene and were resistant to methicillin. The remaining 124 isolates were methicillin susceptible and did not have the mecA gene. Cefoxitin MICs of all isolates were detected by NRA, REMA, and reference broth microdilution methods. Category and essential agreement were determined as 100% and 99.6%, respectively, comparing both NRA and REMA with the reference method. No minor, major, or very major discrepancy was observed for either of the methods. The time necessary to have the MIC results was 5 h for NRA, whereas it was 6 h for REMA. Early detection of MRSA is an important public health concern, and the results of this study showed that both of the colorimetric methods are easy to perform and save time in the determination of MRSA. These methods have a potential use for early detection of MRSA for laboratories unable to use molecular techniques.
authors.affiliation: Ondokuz Mayis University, Medical School, Department of Medical Microbiology, Samsun, Turkey. cobanay2003@gmail.com
authors.forename: Ahmet Yilmaz
authors.initials: AY
authors.lastname: Coban
mesh_terms: D003124:Colorimetry / Q000379:methods*; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests / Q000379:methods; D050901:Nitrate Reductase / Q000378:metabolism; D010078:Oxazines / Q000378:metabolism; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D013997:Time Factors; D014966:Xanthenes / Q000378:metabolism
chemical_list: D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D050901:Nitrate Reductase