title: Imaging therapeutic PARP inhibition in vivo through bioorthogonally developed companion imaging agents.
abstract: A number of small-molecule poly (ADP-ribose) polymerase (PARP) inhibitors are currently undergoing advanced clinical trials. Determining the distribution and target inhibitory activity of these drugs in individual subjects, however, has proven problematic. Here, we used a PARP agent for positron emission tomography-computed tomography (PET-CT) imaging ((18)F-BO), which we developed based on the Olaparib scaffold using rapid bioorthogonal conjugation chemistries. We show that the bioorthogonal (18)F modification of the parent molecule is simple, highly efficient, and well tolerated, resulting in a half maximal inhibitory concentration (IC(50)) of 17.9 ± 1.1 nM. Intravital imaging showed ubiquitous distribution of the drug and uptake into cancer cells, with ultimate localization within the nucleus, all of which were inhibitable. Whole-body PET-CT imaging showed tumoral uptake of the drug, which decreased significantly, after a daily dose of Olaparib. Standard (18)F-fludeoxyglucose imaging, however, failed to detect such therapy-induced changes. This research represents a step toward developing a more generic approach for the rapid codevelopment of companion imaging agents based on small-molecule therapeutic inhibitors.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Reiner
authors.forename: Jessica
authors.initials: J
authors.lastname: Lacy
authors.forename: Edmund J
authors.initials: EJ
authors.lastname: Keliher
authors.forename: Katherine S
authors.initials: KS
authors.lastname: Yang
authors.forename: Adeeti
authors.initials: A
authors.lastname: Ullal
authors.forename: Rainer H
authors.initials: RH
authors.lastname: Kohler
authors.forename: Claudio
authors.initials: C
authors.lastname: Vinegoni
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008958:Models, Molecular; D057054:Molecular Imaging; D064847:Multimodal Imaging; D009369:Neoplasms / Q000175:diagnosis / Q000188:drug therapy; D010793:Phthalazines / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010879:Piperazines / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000737:chemistry; D049268:Positron-Emission Tomography; D011485:Protein Binding; D011487:Protein Conformation; D019275:Radiopharmaceuticals / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D014057:Tomography, X-Ray Computed
chemical_list: D004791:Enzyme Inhibitors; D010793:Phthalazines; D010879:Piperazines; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D019275:Radiopharmaceuticals; D011065:Poly(ADP-ribose) Polymerases; C531550:olaparib