title: Quantitative proteomic study of human lung squamous carcinoma and normal bronchial epithelial acquired by laser capture microdissection.
abstract: OBJECTIVE
  To investigate the differential protein profile of human lung squamous carcinoma (HLSC) and normal bronchial epithelium (NBE) and provide preliminary results for further study to explore the carcinogenic mechanism of HLSC.
  
  
  METHODS
  Laser capture microdissection (LCM) was used to purify the target cells from 10 pairs of HLSC tissues and their matched NHBE, respectively. A stable-isotope labeled strategy using iTRAQ, followed by 2D-LC/Q-STAR mass spectrometry, was performed to separate and identify the differential expression proteins.
  
  
  RESULTS
  A total of 96 differential expression proteins in the LCM-purified HLSC and NBE were identified. Compared with NBE, 49 proteins were upregulated and 47 proteins were downregulated in HLSC. Furthermore, the expression levels of the differential proteins including HSPB1, CKB, SCCA1, S100A8, as well as S100A9 were confirmed by western blot and tissue microarray and were consistent with the results of quantitative proteomics.
  
  
  CONCLUSIONS
  The different expression proteins in HLSC will provide scientific foundation for further study to explore the carcinogenic mechanism of HLSC.
authors.affiliation: Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Lan-Qin
authors.initials: LQ
authors.lastname: Cao
authors.forename: Long-Yu
authors.initials: LY
authors.lastname: Jin
authors.forename: Zhu-Chu
authors.initials: ZC
authors.lastname: Chen
authors.forename: Gu-Qing
authors.initials: GQ
authors.lastname: Zeng
authors.forename: Can-E
authors.initials: CE
authors.lastname: Tang
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Li
authors.forename: Chao-Jun
authors.initials: CJ
authors.lastname: Duan
authors.forename: Fang
authors.initials: F
authors.lastname: Peng
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Xiao
authors.forename: Cui
authors.initials: C
authors.lastname: Li
mesh_terms: D000704:Analysis of Variance; D000951:Antigens, Neoplasm / Q000032:analysis / Q000378:metabolism; D015153:Blotting, Western; D001980:Bronchi / Q000166:cytology; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007553:Isotope Labeling; D061346:Laser Capture Microdissection / Q000379:methods*; D008175:Lung Neoplasms / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D020545:Respiratory Mucosa / Q000737:chemistry / Q000378:metabolism*; D015843:Serpins / Q000032:analysis / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins; D015843:Serpins; C051072:squamous cell carcinoma-related antigen