title: Study on the interference of rat feed to pharmacokinetics of flavonoids.
abstract: OBJECTIVE
  To investigate the interference of rat feed to pharmacokinetic of flavonoids.
  
  
  METHODS
  Flavonoids in rat feed and plasma samples were separated by rapid resolution reversed-phase HPLC and characterized by LC-ESI-MS/MS analysis with an electrospray ionization source (ESI) and a triple quadrupole analyzer by fragmentation pattern.
  
  
  RESULTS
  Five Citrus flavonoids (naringin, naringenin, quercetin, hesperidin and hesperetin) and two soybean isoflavonoids (daizin,daidzein) were identified in common formula rat feed. The presence of these flavonoids in plasma from rats receiving the feed was also confirmed.
  
  
  CONCLUSIONS
  The results showed the flavonoids in animal food might interfere with pharmacological and pharmacokinetic study of foreign natural compounds. This paper could be taken as a reference to pharmacological and pharmacokinetic studies of all the related substances.
authors.affiliation: School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. yangsophia76@hotmail.com
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Yang
authors.forename: Meng-Hua
authors.initials: MH
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Zou
authors.forename: Si-Qi
authors.initials: SQ
authors.lastname: Fang
authors.forename: Wei wei
authors.initials: Ww
authors.lastname: Su
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry; D044950:Flavanones / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D007529:Isoflavones / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D044950:Flavanones; D007529:Isoflavones