title: Gene expression analysis of membrane transport proteins in normal and lipopolysaccharide-inflamed rat dental pulp.
abstract: BACKGROUND
  Membrane transport proteins (transporters) play a crucial role in the transmembrane uptake and/or efflux of various compounds such as inorganic ions, endogenous bioactive substances such as prostaglandins (PGs), and drugs such as nonsteroidal anti-inflammatory drugs. This study aimed to analyze mRNA expression of selected transporters related to drug disposition and PG transport in normal and lipopolysaccharide (LPS)-inflamed rat incisor pulp.
  
  
  METHODS
  Pulp tissues were subjected to reverse transcription-polymerase chain reaction (PCR) detection for transporter isoforms belonging to organic anion transporting polypeptide (Oatp), organic anion transporter (Oat), organic cation transporter (Oct), multidrug resistance-associated protein (Mrp), and multidrug resistance protein (Mdr) families. The levels of mRNA expression for PG transporters (Oatp1a5, Oatp1b2, Oatp2a1, Oatp2b1, and Oatp3a1) were compared in normal and LPS-inflamed pulps by using real-time PCR.
  
  
  RESULTS
  The pulp tissue expressed mRNAs for various transporters belonging to the Oatp, Oat, Oct, Mrp, and Mdr families. LPS inflammation caused significant up-regulation of Oatp2a1 (P < .01) and significant down-regulation of Oatp1a5, Oatp2b1 (P < .01), and Oatp3a1 (P < .05).
  
  
  CONCLUSIONS
  Rat incisor dental pulp expressed mRNAs for various transporter isoforms. The levels of mRNA expression for PG transporters were significantly up-regulated or down-regulated in LPS-inflamed dental pulp.
authors.affiliation: Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Ohkura
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Shigetani
authors.forename: Nagako
authors.initials: N
authors.lastname: Yoshiba
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Yoshiba
authors.forename: Takashi
authors.initials: T
authors.lastname: Okiji
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000032:analysis; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism; D050482:Catecholamine Plasma Membrane Transport Proteins / Q000032:analysis; D003782:Dental Pulp / Q000378:metabolism*; D020869:Gene Expression Profiling; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics*; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000032:analysis; D027361:Organic Anion Transporters / Q000032:analysis; D029362:Organic Anion Transporters, Sodium-Independent / Q000032:analysis; D027701:Organic Cation Transport Proteins / Q000032:analysis; D011453:Prostaglandins / Q000378:metabolism; D020033:Protein Isoforms / Q000032:analysis; D011671:Pulpitis / Q000139:chemically induced / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D000074542:Solute Carrier Organic Anion Transporter Family Member 1B3; D013047:Specific Pathogen-Free Organisms; D000097202:ATP-Binding Cassette Sub-Family B Member 4
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D000894:Anti-Inflammatory Agents, Non-Steroidal; D050482:Catecholamine Plasma Membrane Transport Proteins; D008070:Lipopolysaccharides; D026901:Membrane Transport Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D027361:Organic Anion Transporters; D029362:Organic Anion Transporters, Sodium-Independent; D027701:Organic Cation Transport Proteins; D011453:Prostaglandins; D020033:Protein Isoforms; D012333:RNA, Messenger; D000074542:Solute Carrier Organic Anion Transporter Family Member 1B3; D000097202:ATP-Binding Cassette Sub-Family B Member 4; C489542:Abcc4 protein, rat; C492375:Abcc5 protein, rat; C493212:Slc22a1 protein, rat; C421207:Slco1b2 protein, rat; C533062:Slco2b1 protein, rat; C114338:organic anion transport protein 3; C095122:multidrug resistance protein 3; C421109:multidrug resistance-associated protein 1