title: Adiponectin improves endothelial dysfunction caused by elevated FFAs levels, partially through cAMP-dependent pathway.
abstract: OBJECTIVE
  To determine whether adiponectin can attenuate endothelial dysfunction caused by elevated free fatty acids (FFAs) concentration, and if so, to explore the underlying mechanism.
  
  
  METHODS
  Male Sprague-Dawley rat thoracic aortas were isolated then cut into four vascular rings, incubated in organ bath containing Krebs-Henseleit buffer with different agents separately: 800 μmol/L Palmic acid (FFA, n = 14), 800 μmol/L Palmic acid + 2 μg/mL adiponectin (FFA + gAd, n = 14), 800 μmol/L Palmic acid + 2 μg/mL adiponectin + 200 μmol/L adenylate cyclase inhibitor dideoxyadenosine (FFA + gAd + ddAdo, n = 7), blank control (NC, n=10). The endothelial dependent vasodilatation (EDV) and endothelial independent vasodilatation (EIV) were assessed by acetylcholine (Ach) induced contraction of the aortas. Nuclear transcription factor kappa B (NF-κB) expression in rat aortic section was evaluated immunohistochemically.
  
  
  RESULTS
  Ach caused a concentration dependent vascular relaxation in all pre-constricted aortic rings. PA treatment impaired the Ach induced EDV which was significantly attenuated by pretreatment with adiponectin. Dideoxyadenosine partly abolished the vascular protective effect of adiponectin. Sodium nitroprusside (SNP) had no significant effect on the vasodilatation among four groups. Increased NF-κB expression was noted in FFA group. Pretreatment with adiponectin partly decreased NF-κB expression when compared with FFA group.
  
  
  CONCLUSIONS
  Adiponectin may independently mitigate endothelial dysfunction caused by elevated FFAs concentration through the cross talk between cAMP and NF-κB signaling pathway.
authors.affiliation: Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu City, Sichuan Province, China.
authors.forename: Ben
authors.initials: B
authors.lastname: Wang
authors.forename: Yerong
authors.initials: Y
authors.lastname: Yu
authors.forename: Lina
authors.initials: L
authors.lastname: Han
mesh_terms: D052242:Adiponectin / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D000242:Cyclic AMP / Q000378:metabolism*; D042783:Endothelial Cells / Q000187:drug effects / Q000473:pathology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D016328:NF-kappa B / Q000378:metabolism* / Q000494:pharmacology; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D052242:Adiponectin; D005230:Fatty Acids, Nonesterified; D016328:NF-kappa B; D014665:Vasodilator Agents; D000242:Cyclic AMP