title: [Study on the effect of high hydrostatic pressure treatment on the secondary structure of mushroom polyphenoloxidase by SRCD and FTIR].
abstract: The secondary structure of the mushroom polyphenoloxidase treated by the high hydrostatic pressure (HHP) was analyzed by the synchrotron radiation circular dichroism (SRCD) and Fourier transform infrared spectroscopy (FTIR). The alpha-helix content of mushroom PPO was decreased after HHP treatment, which indicated that the secondary structure of PPO was changed. There was a discrepancy of the result of the secondary structure content between untreated or HHP-treated mushroom PPO analyzed by SRCD and FTIR spectra, and this discrepancy may be due to the different determination temperature, the concentration of the PPO solution and the spectra analysis method etc. The fluorescence spectra showed that the fluorescence intensity of the mushroom PPO was decreased after HHP treatment, and a red shift was observed after HHP treatment, which indicated that the tertiary structure of the enzyme molecule has been modified.
authors.affiliation: National Engineering Research Centre for Fruit and Vegetable Processing, Beijing 100083, China. yijianyong515@126.com
authors.forename: Jian-yong
authors.initials: JY
authors.lastname: Yi
authors.forename: Peng
authors.initials: P
authors.lastname: Dong
authors.forename: Yong-tao
authors.initials: YT
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Jiang
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Liao
authors.forename: Xiao-song
authors.initials: XS
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000363:Agaricales; D004156:Catechol Oxidase; D002942:Circular Dichroism; D005453:Fluorescence; D006874:Hydrostatic Pressure; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared; D017356:Synchrotrons
chemical_list: D004156:Catechol Oxidase