title: Visual and on-site detection of mercury(II) ions on lateral flow strips using DNA-functionalized gold nanoparticles.
abstract: A test strip for detection of Hg(2+) in aqueous solution based on the DNA-functionalized gold nanoparticles (DNA-AuNPs) was developed and evaluated.  When Hg(2+) ions were introduced, the biotinylated DNA(2) hybridized with thiolated DNA(1) functionalized on the AuNPs (DNA(1)-AuNPs) to form mismatch complexes through thymine-Hg(2+)-thymine (T-Hg(2+)-T) coordination.  The formed mismatch complexes and excess DNA(1)-AuNPs could be captured on the test line formed by streptavidin and the control line formed by DNA(3)-BSA, respectively.  Two red lines appeared due to the accumulation of AuNPs, enabling visual detection of Hg(2+) with a detection limit of about 6 nM.  The assay results can be obtained within 5 min.  The results show that the test strip has excellent sensitivity and selectivity for detection of Hg(2+); thus it holds a great potential for rapid, on-site and real time detection of Hg(2+).
authors.affiliation: Faculty of Materials Science and Chemical Engineering, Ningbo University, Zhejiang, P. R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Duan
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Zebo
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guo
mesh_terms: D019921:Biotinylation; D002021:Buffers; D004247:DNA / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D007477:Ions; D008628:Mercury / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013997:Time Factors; D014873:Water Pollutants / Q000032:analysis
chemical_list: D002021:Buffers; D007477:Ions; D014873:Water Pollutants; D012710:Serum Albumin, Bovine; D006046:Gold; D004247:DNA; D008628:Mercury