title: [Construction of an expression vector with elastin-like polypeptide tag to purify xylanase].
abstract: OBJECTIVE
  This paper reports the purification of xylanase using the shortest, sensitive ELP [KV8F-20].
  
  
  METHODS
  We designed a thermophilic xylanase gene, and recombined it with the ELP via a random coil sequence to generate the vector pET-22b-SoxB-M2-S-ELP. The expressed xylanase was purified by inverse transition cycling through high-speed centrifugation, and then we characterized the purified xylanase.
  
  
  RESULTS
  The phase transition temperature of the ELPs dropped to 22 degrees C with 0.5 mol/L sodium carbonate (pH = 7). Under this condition, SoxB-M2-S-ELP was purified by 3.16 folds after centrifugation. The recovery rate was 21.2%, and purity of the xylanase was 64.3%.
  
  
  CONCLUSIONS
  Elastin-like polypeptide as a purification tag to purify recombinant proteins is simple, fast, gentle and cheaper. The expression vector we constructed here might be a very useful and reliable tool to purify many other target proteins.
authors.affiliation: Department of Bioengineering and Biotechnology, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China. 54maozhu@163.com
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Fu
authors.forename: Wenyan
authors.initials: W
authors.lastname: Wang
authors.forename: Guangya
authors.initials: G
authors.lastname: Zhang
mesh_terms: D004549:Elastin / Q000235:genetics*; D043364:Endo-1,4-beta Xylanases / Q000302:isolation & purification*; D005822:Genetic Vectors; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000235:genetics*; D044367:Phase Transition; D013696:Temperature
chemical_list: D010455:Peptides; D004549:Elastin; D043364:Endo-1,4-beta Xylanases