title: Rapid whole protein quantitation of staphylococcal enterotoxins A and B by liquid chromatography/mass spectrometry.
abstract: Staphylococcus aureus is an important pathogen and has been indicated as the fifth causative agent of food-borne human illness throughout the world. Staphylococcal enterotoxins (SEs) are toxic compounds excreted mainly by strains of S. aureus. Among these toxins, enterotoxins A (SEA) and B (SEB) are both of the most prevalent compounds in staphylococcal food poisoning. In this work, reverse phase liquid chromatography coupled to ESI mass spectrometry (LC-ESI/MS) has been applied for its rapid identification and quantification. Limit of detection (LOD) values were 0.5 and 0.2 ng for SEA and SEB, respectively and limit of quantification (LOQ) value was 1 ng for both enterotoxins. SEA and SEB have been analyzed as intact proteins in milk and fruit juices. Analytical methods are essential for routine monitoring purposes and safeguard public health and the proposed technique can detect and quantify successfully SEA and SEB in food samples.
authors.affiliation: Department of Preventive Medicine and Public Health, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain. m.isabel.sospedra@uv.es
authors.forename: Isabel
authors.initials: I
authors.lastname: Sospedra
authors.forename: Carla
authors.initials: C
authors.lastname: Soler
authors.forename: Jordi
authors.initials: J
authors.lastname: Mañes
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Soriano
mesh_terms: D000818:Animals; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D032084:Citrus sinensis / Q000737:chemistry; D004768:Enterotoxins / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D008892:Milk / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal