title: [Monitoring of Epstein-Barr virus-infected cells by flow cytometer permits early diagnosis and evaluation of disease progression in EBV-associated hemophagocytic lymphohistiocytosis].
abstract: Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis (EBV-HLH) is characterized by clonal expansion of EBV-infected CD8(+)T-cells. We have recently demonstrated that detection of a clonally expanded population of EBV-infected CD8(+)T-cells with CD5 down-regulation was a useful tool to distinguish EBV-HLH from EBV-related disorders such as severe infectious mononucleosis. A 5-year-old girl who presented with fever, pancytopenia and liver dysfunction was diagnosed by this method in addition to conventional diagnostic tests. Further, EBV-infected cells were identified as CD5(-)HLA-DR(+) TCR V β3(+) CD8(+)T cells, an increase or decrease of which over time reflected the disease severity in this patient. Treatment of patients with EBV-HLH varies from steroid alone to intensive chemotherapy or hematopoietic stem cell transplantation. Easy monitoring of EBV-infected cells by using flow cytometry over time may provide useful information to choose an appropriate treatment for each individual patient with EBV-HLH.
authors.affiliation: Department of Pediatrics, Kanazawa University.
authors.forename: Masaki
authors.initials: M
authors.lastname: Fukuda
authors.forename: Ryosei
authors.initials: R
authors.lastname: Nishimura
authors.forename: Raita
authors.initials: R
authors.lastname: Araki
authors.forename: Rie
authors.initials: R
authors.lastname: Kuroda
authors.forename: Shintaro
authors.initials: S
authors.lastname: Mase
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Yumi
authors.initials: Y
authors.lastname: Tone
authors.forename: Hideaki
authors.initials: H
authors.lastname: Maeba
authors.forename: Taizo
authors.initials: T
authors.lastname: Wada
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Kasahara
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yachie
mesh_terms: D015415:Biomarkers; D018956:CD5 Antigens; D018414:CD8-Positive T-Lymphocytes / Q000821:virology*; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D018450:Disease Progression; D042241:Early Diagnosis; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000175:diagnosis*; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens; D004854:Herpesvirus 4, Human; D006801:Humans; D051359:Lymphohistiocytosis, Hemophagocytic / Q000150:complications / Q000175:diagnosis* / Q000821:virology*
chemical_list: D015415:Biomarkers; D018956:CD5 Antigens; D004279:DNA, Viral; D006684:HLA-DR Antigens