title: Environmental exposure to organophosphate pesticides: assessment of endocrine disruption and hepatotoxicity in pregnant women.
abstract: In utero exposure is the first point of contact with environmental xenobiotics that may affect the maternal-placental-fetal balance. Considering that maternal pathophysiological changes affect intrauterine development, this pilot study was conducted to address how environmental exposure to organophosphate pesticides (OPs) during pregnancy may contribute to maternal endocrine disruption and disturbed hepatic function. A prospective study was carried out with pregnant women (n=97) living in a rural area of the Rio Negro province where OPs are intensively applied throughout 6 months of the year. Blood samples were obtained and biomarkers of OPs exposure (cholinesterases and β-glucuronidase), cortisol (CT) and progesterone (PG) levels, as well as glycemia, were determined. Parameters of liver injury were assayed by measuring aspartate aminotransferase (AST) and alanine aminotransferase (ALT); liver function was assayed by measuring albumin. Biomonitoring carried out during the pre-spraying period (PreS) and spraying period (SP) showed that the population studied was exposed to OPs, proven by the fact that plasma (PCh) and erythrocyte cholinesterase (AChE) decreased very significantly (p<0.01) during SP. CT values increased very significantly (p<0.01) in the first trimester of pregnancy during SP with respect to PreS. Individual values above the upper limit of the CT and PG reference range were found both in PreS and SP. This finding could be associated with changes in hormone metabolism pathways produced by OPs exposure. During the second trimester of pregnancy there were increases in ALT values and the AST/ALT ratio in SP, suggesting subclinical hepatotoxicity. In SP, glycemia was unchanged while albuminemia increased. Although anthropometric newborn parameters and pregnancy alterations were within normal values for the general population, the increase in CT in the maternal compartment may lead to impaired newborn health later in life.
authors.affiliation: Hospital Dr. Ernesto Accame, Ing Quesnel S/N°, Allen (8328), Río Negro, Argentina. amaliacecchi@gmail.com
authors.forename: A
authors.initials: A
authors.lastname: Cecchi
authors.forename: M G
authors.initials: MG
authors.lastname: Rovedatti
authors.forename: G
authors.initials: G
authors.lastname: Sabino
authors.forename: G G
authors.initials: GG
authors.lastname: Magnarelli
mesh_terms: D000293:Adolescent; D000328:Adult; D000410:Alanine Transaminase / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D001938:Brazil; D002802:Cholinesterases / Q000097:blood; D052244:Endocrine Disruptors / Q000097:blood* / Q000633:toxicity; D004703:Endocrine System / Q000187:drug effects; D004781:Environmental Exposure / Q000032:analysis* / Q000706:statistics & numerical data; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D008099:Liver / Q000187:drug effects; D009943:Organophosphorus Compounds / Q000097:blood* / Q000633:toxicity; D010575:Pesticides / Q000097:blood* / Q000633:toxicity; D010865:Pilot Projects; D011247:Pregnancy; D037841:Pregnant People; D011374:Progesterone / Q000097:blood; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D015415:Biomarkers; D052244:Endocrine Disruptors; D009943:Organophosphorus Compounds; D010575:Pesticides; D011374:Progesterone; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D002802:Cholinesterases; D005966:Glucuronidase; D006854:Hydrocortisone