title: [Effect on elimination of pyrogen and effectual components of Panax notoginseng saponins by ultrafiltration membranes of different cut-off molecular weight and different materials].
abstract: OBJECTIVE
  To study the elimination effect of bacterial endotoxins and the transmittance of Panax notoginseng saponins by ultrafiltration membranes of different cut-off molecular weight and different materials.
  
  
  METHODS
  The kinetic-turbidimetric method was used to determine the content of bacterial endotoxins in Panax notoginseng saponins solution before and after using the ultrafiltration. The change of the contents of active components was examined by HPLC,using notoginsennoside R1, ginsennoside Rg1, ginsennoside Rb1 and ginsennoside Rd as the mark components.
  
  
  RESULTS
  The removal rate of bacterial endotoxin fell along with the increasing of membrane aperture. The removal rate was 20. 69% by ultrafiltration membranes of 100 KDa with polysulfone material,less than those of other ultrafiltration membranes with polysulfone material. But the removal rate of bacterial endotoxin by E membranes of blend materials was higher than those of other ultrafiltration membranes with polysulfone material. The contents of active components filtered by E membranes of blend materials was more than that of ultrafiltration membranes of 100 KDa with polysulfone material.
  
  
  CONCLUSIONS
  The applicability of ultrafiltration membranes of large cut-off molecular weight and blend materials of effectual component in Panax notoginseng saponins and elimination of pyrogen is good.
authors.affiliation: School of Pharmacy of Nanjing University of Chinese Medicine,Nanjing 210046, China. lihemin2006@sina.com
authors.forename: He-Min
authors.initials: HM
authors.lastname: Li
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Peng
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Zheng
authors.forename: Cun-Yu
authors.initials: CY
authors.lastname: Li
authors.forename: Tong-Xing
authors.initials: TX
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004731:Endotoxins / Q000032:analysis / Q000302:isolation & purification*; D036145:Ginsenosides / Q000032:analysis*; D008567:Membranes, Artificial; D008970:Molecular Weight; D053723:Panax notoginseng / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011749:Pyrogens / Q000032:analysis / Q000302:isolation & purification; D012503:Saponins / Q000737:chemistry*; D013450:Sulfones / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods*
chemical_list: D004731:Endotoxins; D036145:Ginsenosides; D008567:Membranes, Artificial; D011108:Polymers; D011749:Pyrogens; D012503:Saponins; D013450:Sulfones; C017662:polysulfone P 1700; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1