title: [Determination of baclofen using micellar electrokinetic chromatography with laser induced fluorescence detection].
abstract: A novel micellar electrokinetic chromatographic method with laser induced fluorescence detection after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) was developed for the determination of muscle relaxant drug baclofen (BAL). After optimization, baseline separation of the derivatives of BAL and gabapentin (internal standard) was obtained within 7 min in a running buffer (pH 9.75) composed of 15 mmol/L sodium borate, 20 mmol/L sodium dodecyl sulfate and 10% (v/v) acetonitrile. The separation voltage was 17.5 kV. The column temperature was 25 degrees C. The samples were injected by a pressure of 3.45 kPa (0.5 psi) for 3 s. The method has a linear range of 0.025 - 25 mg/L for BAL with the correlation coefficient of 0.999 9. The limit of detection (LOD, S/N = 3) and the limit of quantification (LOQ, S/N = 10) were 0.90 microg/L and 6.25 microg/L, respectively. The developed method was used for the analysis of BAL pharmaceutical preparation and urine samples spiked with BAL standard. The ranges of recovery were 101.6% - 107.9% for BAL preparation and 107.0% - 109.6% for urine samples. This method can be applied to the quality assessment of baclofen drug products, and provide supplementary means for the drug metabolism research of baclofen.
authors.affiliation: College of Chemical Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Wang
authors.forename: Jiajia
authors.initials: J
authors.lastname: Yang
authors.forename: Yuanli
authors.initials: Y
authors.lastname: Cai
authors.forename: Xia
authors.initials: X
authors.lastname: Lin
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D001418:Baclofen / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D007834:Lasers; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
chemical_list: D001418:Baclofen