title: Antagonistic effect of disulfide-rich peptide aptamers selected by cDNA display on interleukin-6-dependent cell proliferation.
abstract: Several engineered protein scaffolds have been developed recently to circumvent particular disadvantages of antibodies such as their large size and complex composition, low stability, and high production costs. We previously identified peptide aptamers containing one or two disulfide-bonds as an alternative ligand to the interleukin-6 receptor (IL-6R). Peptide aptamers (32 amino acids in length) were screened from a random peptide library by in vitro peptide selection using the evolutionary molecular engineering method "cDNA display". In this report, the antagonistic activity of the peptide aptamers were examined by an in vitro competition enzyme-linked immunosorbent assay (ELISA) and an IL-6-dependent cell proliferation assay. The results revealed that a disulfide-rich peptide aptamer inhibited IL-6-dependent cell proliferation with similar efficacy to an anti-IL-6R monoclonal antibody.
authors.affiliation: Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Saitama 338-8570, Japan. nemoto@fms.saitama-u.ac.jp
authors.forename: Naoto
authors.initials: N
authors.lastname: Nemoto
authors.forename: Chihiro
authors.initials: C
authors.lastname: Tsutsui
authors.forename: Junichi
authors.initials: J
authors.lastname: Yamaguchi
authors.forename: Shingo
authors.initials: S
authors.lastname: Ueno
authors.forename: Masayuki
authors.initials: M
authors.lastname: Machida
authors.forename: Toshikatsu
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Takafumi
authors.initials: T
authors.lastname: Sakai
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003545:Cysteine / Q000737:chemistry; D003553:Cystine / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D057166:High-Throughput Screening Assays; D006801:Humans; D008969:Molecular Sequence Data; D019151:Peptide Library; D012641:Selection, Genetic; D000093242:Interleukin-6 Inhibitors
chemical_list: D052158:Aptamers, Peptide; D003545:Cysteine; D003553:Cystine; D018076:DNA, Complementary; D019151:Peptide Library; D000093242:Interleukin-6 Inhibitors