title: Platelet-rich plasma reverses the inhibition of tenocytes and osteoblasts in tendon-bone healing.
abstract: The purpose of this study was to investigate the effect of platelet-rich plasma on the proliferation of osteoblasts and tenocytes in tendon-bone healing. We cultured osteoblasts and tenocytes in an indirect coculture system with Transwell filters (Merck Millipore, Billerica, Massachusetts). The proliferation was examined using Cell Counting Kit-8 (Dojindo Chemistry Research Institute, Kumamoto, Japan).Four groups were studied: group 1, one cell type cultured without platelet-rich plasma; group 2, two cell types cultured together in an indirect coculture system without platelet-rich plasma; group 3, cells in the outer chamber and platelet-rich plasma in the inner chamber; and group 4, two different cell types in each of the 2 chambers with platelet-rich plasma in the inner chamber. The proliferation rates of groups 3 and 4 were the highest, followed by group 1 and then group 2, which was the lowest.Platelet-rich plasma abolishes the inhibition of osteoblasts or tenocytes in an indirect coculture system and improves the cell proliferation rate.
authors.affiliation: Department of Orthopedic Surgery, Southeast Hospital Affiliated to Xiamen University, Zhanghou, China. 1787257385@qq.com
authors.forename: Wenliang
authors.initials: W
authors.lastname: Zhai
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Qi
authors.forename: Quan
authors.initials: Q
authors.lastname: Gao
authors.forename: Liren
authors.initials: L
authors.lastname: Yi
mesh_terms: D001842:Bone and Bones / Q000166:cytology* / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D012038:Regeneration / Q000502:physiology; D013710:Tendons / Q000166:cytology* / Q000502:physiology*