title: Cell population based mass spectrometry using platinum nanodots for algal and fungal studies.
abstract: For the first time, we applied cell-population based mass spectrometry (CP-MS) for biosensing intact eukaryotic cells of Chlamydomonas reinhardtii and Saccharomyces cerevisiae. Cell counts ranging from 1 × 10(7) to 1.28 × 10(2) were analyzed using MALDI-MS to obtain the threshold detection sensitivity. Platinum nanodots (Pt NDs) were used to enhance the detection sensitivity of CP-MS. Pt NDs were able to improve the detection sensitivity of CP-MS from 3200 cells/mL to 640 cells/mL (5-fold) for Chlamydomonas. For yeast cells, the detection sensitivity was also increased from 400,000 cells/mL to 3200 cells/mL (125-fold) when Pt NDs were used. Using the Clin Pro tool, the obtained results from MALDI-MS data were validated. Statistical analysis of the mass data was performed using MYSTAT software.
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan.
authors.forename: Muthu
authors.initials: M
authors.lastname: Manikandan
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan. Electronic address: hwu@faculty.nsysu.edu.tw.
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Wu
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan.
authors.forename: Nazim
authors.initials: N
authors.lastname: Hasan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D016825:Chlamydomonas reinhardtii / Q000166:cytology; D015169:Colony Count, Microbial / Q000379:methods*; D053768:Metal Nanoparticles / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D010984:Platinum; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010984:Platinum