title: [Simultaneous determination of 10 sulfonamide antibiotics in water by solid-phase extraction and high performance liquid chromatography].
abstract: A solid-phase extraction (SPE)-high performance liquid chromatography (HPLC) method has been developed for simultaneous determination of 10 sulfonamide antibiotics in water. The analytes were first enriched and purified through a PEP solid-phase extraction column, and eluted with acetonitrile-dichloromethane solution (2: 1, V/V), then detected by a HPLC with a UV detector. The detection wavelength was 268 nm and the column temperature was 33 degrees C, using gradient elution process with acetonitrile - 0.4% acetic acid/water (V/V) as the mobile phase to achieve baseline separations of these 10 analytes. The linearity range was 10 - 2 000 microg x L(-1). The recovery ranges of standard addition for deionized water and real water samples were 73.4% - 95.6% and 70.2% - 92.5%, respectively (except for sulfonamide, were 8.5% and 8.0%). The limit of detection was 1.42-7.25 ng x L(-1). Application of this method for parts of Huangpu River in Shanghai, surface water and groundwater in Chongming Island showed that sulfonamide antibiotics were detected in different frequencies in different aqueous environments, with the concentration range of 13.3 - 241.5 ng x L(-1), proving this method is easy, rapid, sensitive and efficient to meet the needs of actual work.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China. njhhhlj@163.com
authors.forename: Lei-Jie
authors.initials: LJ
authors.lastname: Hong
authors.forename: Lu
authors.initials: L
authors.lastname: Shi
authors.forename: Ya-Lei
authors.initials: YL
authors.lastname: Zhang
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Zhou
authors.forename: Hong-Guang
authors.initials: HG
authors.lastname: Zhu
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Lin
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000145:classification; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D060587:Groundwater / Q000032:analysis; D045483:Rivers; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D013449:Sulfonamides / Q000032:analysis* / Q000145:classification; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D013449:Sulfonamides; D014874:Water Pollutants, Chemical