title: A live birth of activated one-day-old unfertilized oocyte for a patient who experienced repeatedly near-total fertilization failure after intracytoplasmic sperm injection.
abstract: Total or near-total fertilization failure after intracytoplasmic sperm injection (ICSI) is a rare event, but it occurs repeatedly because of sperm defects in activating oocyte. The case presents a successful pregnancy and live birth after calcium ionophore A23187 (A23187) activation on one-day-old unfertilized oocytes in a patient whose husband suffered oligoasthenoteratozoospermia, and who had experienced repeated near-total fertilization failure after ICSI. In the second ICSI cycle, only one oocyte was fertilized while nine were unfertilized. Oocyte activation with A23187 were performed on the one-day-old unfertilized oocytes after ICSI and resulted in fertilization and embryo transfer. A clinical pregnancy was achieved and a healthy baby was born. To our knowledge, this is the first reported case of a healthy birth after oocyte activation on the one-day-old unfertilized oocyte. This indicates that "rescue oocyte activation" on one-day-old unfertilized oocytes after ICSI may be helpful for preventing total or near-total fertilization failure after ICSI.
authors.affiliation: Reproductive Medical Center, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Lu
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Liang
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Zhao
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Wei
authors.forename: Huan
authors.initials: H
authors.lastname: Shen
mesh_terms: D000328:Adult; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D050498:Live Birth; D008297:Male; D009865:Oocytes / Q000166:cytology*; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*