title: Protection of mice against X-ray injuries by the post-irradiation administration of inosine-5'-monophosphate.
abstract: The aim of the present study was to investigate the radiation modulating properties of inosine-5'-monophosphate (IMP). Mice injected introperitoneally (i.p.) with IMP 15 minutes after irradiation with a lethal irradiation dose of 7 Gy have better survival rates comparative to irradiated mice non treated with IMP. The dose reduction factor of the IMP is 1.22. Using a hematologdical test we demonstrated that administration of IMP alleviates the symptoms of radiation-induced leukopenia and thrombocytopenia. The DNA damage in bone marrow and thymus cells of irradiated mice was measured by flow cytofluorometry and micronucleus test (MN-test). The tests show that i.p. administration of IMP to irradiated animals leads to a significant reduction of the DNA damage level. In this paper we show that IMP substantially modulates the damaging effects of ionizing radiation protecting irradiated mice and it is a promising agent for a treatment of leukopenia.
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Nelli R
authors.initials: NR
authors.lastname: Asadullina
authors.forename: Anna M
authors.initials: AM
authors.lastname: Usacheva
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Gudkov
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D004249:DNA Damage / Q000187:drug effects*; D007263:Infusions, Parenteral; D007291:Inosine Monophosphate / Q000008:administration & dosage*; D007970:Leukopenia / Q000209:etiology / Q000235:genetics* / Q000517:prevention & control*; D008297:Male; D051379:Mice; D011832:Radiation Injuries / Q000175:diagnosis / Q000235:genetics* / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D016896:Treatment Outcome
chemical_list: D011837:Radiation-Protective Agents; D007291:Inosine Monophosphate