title: Partitioning studies of L-glutaminase production by Bacillus cereus MTCC 1305 in different PEG-salt/dextran.
abstract: Partitioning studies of L-glutaminase production by Bacillus cereus MTCC 1305 was carried out in different PEG-salt/PEG-dextran system. The partitioning value of L-glutaminase increased with increasing molecular weight of PEG from 2000-4000 kDa and decreased with higher molecular weight of 6000 kDa. Phase system of PEG 4000 (8.5%)/dextran T500 (9.5%) was selected for the extractive fermentative production of L-glutaminase on the basis maximum partition coefficient (1.31). The production of L-glutaminase was found higher in top phase of ATPS (2.09 U/ml) than control media (1.42 U/ml). Overall production of L-glutaminase (1.83 U/ml) was found lower than top phase (2.09 U/ml) in ATPS system. The growth profile with short lag phase and higher cell concentration was obtained for ATPS. The partition coefficient of L-glutaminase increased with increase of system pH and temperature and optimum production was obtained at pH 7.5 and temperature 30 °C in top phase of PEG 4000/dextran T500 system.
authors.affiliation: School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, Varanasi, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Singh
authors.forename: Rathindra Mohan
authors.initials: RM
authors.lastname: Banik
mesh_terms: D001409:Bacillus cereus / Q000737:chemistry* / Q000201:enzymology*; D003911:Dextrans / Q000737:chemistry*; D005972:Glutaminase / Q000096:biosynthesis* / Q000737:chemistry*; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry*; D012492:Salts / Q000737:chemistry*
chemical_list: D003911:Dextrans; D012492:Salts; D011092:Polyethylene Glycols; D005972:Glutaminase