title: Early antioxidative defence responses in the aquatic worms (Limnodrilus sp.) in Porsuk Creek in Eskisehir (Turkey).
abstract: Certain oligochaeta specimens have been universally applied as bioindicators to reflect the organic and inorganic pollution in rivers and play a major role in the decomposition of pollutants. The aim of this study was to investigate the water quality in Porsuk Creek in Eskisehir (Turkey) through the specimens from two different species that belong to Limnodrilus genus, using their biomonitoring compatibilities for the accumulated trace element concentrations and to describe the applicability of antioxidative systems as biomarkers of pollution in Tubificinae. Therefore, some parameters that serve as biomarkers for antioxidative defence, total protein, glutathione (GSH) contents and glutathione S-transferase (GST) activities, were determined in Limnodrilus hoffmeisteri and Limnodrilus udekemianus. The study was completed with the chemical analysis of the trace elements from these specimens and also from the water samples. As a conclusion, the observed elevation in GSH levels and GST activities reflect the contribution of oxidative stress in toxicity mechanisms due to the accumulation of trace elements, and the study also suggests a general induction of detoxification metabolisms in the presence of several pollutants in benthic sediment-dwelling worms. According to the average value, the trace element levels for two species are as follows: Fe > Al > Zn > Mn > Pb > Cu > Ni > B > Cd = Cr = Hg. As Porsuk Creek is used for many purposes, such as irrigation, drinking water and fish production, discharges of all types of wastes should be under stringent control to avoid the unwanted health effects to its habitants and to humans.
authors.affiliation: Department of Biology, Anadolu University, Eskisehir, Turkey. eoztetik@anadolu.edu.tr
authors.forename: Elif
authors.initials: E
authors.lastname: Oztetik
authors.forename: Arzu
authors.initials: A
authors.lastname: Cicek
authors.forename: Naime
authors.initials: N
authors.lastname: Arslan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D004784:Environmental Monitoring; D005618:Fresh Water / Q000737:chemistry; D019015:Geologic Sediments / Q000737:chemistry; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D000075322:Heavy Metal Poisoning; D019216:Metals, Heavy / Q000032:analysis*; D009835:Oligochaeta / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D014421:Turkey; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000506:poisoning*
chemical_list: D000975:Antioxidants; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D005982:Glutathione Transferase; D005978:Glutathione