title: Catalytic mechanism and kinase interactions of ABA-signaling PP2C phosphatases.
abstract: Abscisic acid (ABA) is an essential hormone that controls plant growth, development and responses to abiotic stresses. ABA signaling is mediated by type 2C protein phosphatases (PP2Cs), including HAB1 and ABI2, which inhibit stress-activated SnRK2 kinases and whose activity is regulated by ABA and ABA receptors. Based on biochemical data and our previously determined crystal structures of ABI2 and the SnRK2.6-HAB1 complex, we present the catalytic mechanism of PP2C and provide new insight into PP2C-SnRK2 interactions and possible roles of other SnRK2 kinases in ABA signaling.
authors.affiliation: Laboratory of Structural Sciences, Van Andel Research Institute, Grand Rapids, MI, USA.
authors.forename: X Edward
authors.initials: XE
authors.lastname: Zhou
authors.forename: Fen-Fen
authors.initials: FF
authors.lastname: Soon
authors.forename: Ley-Moy
authors.initials: LM
authors.lastname: Ng
authors.forename: Amanda
authors.initials: A
authors.lastname: Kovach
authors.forename: Kelly M
authors.initials: KM
authors.lastname: Suino-Powell
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Eu-Leong
authors.initials: EL
authors.lastname: Yong
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
authors.forename: H Eric
authors.initials: HE
authors.lastname: Xu
authors.forename: Karsten
authors.initials: K
authors.lastname: Melcher
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D000071636:Protein Phosphatase 2C; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D046912:Multiprotein Complexes; D010937:Plant Growth Regulators; C492978:SnRK2 protein, Arabidopsis; D000040:Abscisic Acid; D017346:Protein Serine-Threonine Kinases; C106443:ABI2 protein, Arabidopsis; C514757:HAB1 protein, Arabidopsis; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C