title: CD36 promotes adipocyte differentiation and adipogenesis.
abstract: BACKGROUND
  CD36 is a membrane glycoprotein, contributing to the pathogenesis of metabolic disorders, like obesity, which has become a major health concern worldwide.
  
  
  METHODS
  A potential functional role of the scavenger receptor CD36 was investigated in in vitro adipocyte differentiation and in vivo adipogenesis.
  
  
  RESULTS
  During differentiation of 3T3-F442A preadipocytes into mature adipocytes, expression of CD36 was upregulated and CD36 gene silencing resulted in impaired differentiation, as monitored by Oil Red O staining and expression of adipogenic markers. De novo fat pad formation in NUDE mice following injection of preadipocytes was significantly reduced upon CD36 gene silencing as compared to control. This was associated with marked adipocyte hypotrophy and reduced adipose tissue adipocyte content. Macrophage infiltration in de novo fat tissues derived from preadipocytes with CD36 gene silencing was not significantly different from controls. Collagen content was significantly higher in de novo fat with CD36 gene silencing. In a nutritionally induced obesity model, total body weight as well as subcutaneous and gonadal adipose tissue mass were significantly lower in CD36 deficient mice as compared to wild-type littermates.
  
  
  CONCLUSIONS
  Thus, our data support a functional role of CD36 in promoting adipogenesis in vitro as well as in vivo.
authors.affiliation: Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium.
authors.forename: Valerie
authors.initials: V
authors.lastname: Christiaens
authors.forename: Matthias
authors.initials: M
authors.lastname: Van Hul
authors.forename: H Roger
authors.initials: HR
authors.lastname: Lijnen
authors.forename: Ilse
authors.initials: I
authors.lastname: Scroyen
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis / Q000502:physiology*; D000818:Animals; D001835:Body Weight; D018955:CD36 Antigens / Q000737:chemistry / Q000502:physiology*; D002454:Cell Differentiation; D059305:Diet, High-Fat / Q000009:adverse effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008819:Mice, Nude; D009765:Obesity / Q000209:etiology* / Q000517:prevention & control
chemical_list: D018955:CD36 Antigens