title: Cannabinoid exposure in adolescent female rats induces transgenerational effects on morphine conditioned place preference in male offspring.
abstract: In the United States, marijuana is one of the drugs most abused by adolescents, with females representing a growing number of users. In previous studies, treatment of adolescent female rats with morphine significantly altered brain reward systems in future offspring. As both cannabinoid and opioid systems develop during adolescence, it was hypothesized that early exposure to cannabinoids would induce similar transgenerational effects. In the current study, female rats were treated with the cannabinoid receptor (CB1/CB2) agonist WIN 55,212-2 or its vehicle for three consecutive days during adolescent development (30 days of age), and were subsequently mated in adulthood (60 days of age). The adolescent and adult male offspring of these WIN 55,212-2 (WIN-F1)- or vehicle (VEH-F1)-treated females were tested for their response to morphine using the conditioned place preference (CPP) paradigm. Both adolescent and adult WIN-F1offspring exhibited greater sensitivity to morphine CPP than their VEH-F1 counterparts. Collectively, the findings provide additional evidence of transgenerational effects of adolescent drug use.
authors.affiliation: Department of Biomedical Sciences, Tufts University, Cummings School of Veterinary Medicine, North Grafton, MA 01536, USA. john.byrnes@tufts.edu
authors.forename: John J
authors.initials: JJ
authors.lastname: Byrnes
authors.forename: Nicole L
authors.initials: NL
authors.lastname: Johnson
authors.forename: Marian E
authors.initials: ME
authors.lastname: Schenk
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Byrnes
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D063386:Cannabinoid Receptor Agonists / Q000008:administration & dosage / Q000633:toxicity*; D002186:Cannabinoids / Q000008:administration & dosage / Q000633:toxicity*; D002490:Central Nervous System / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D059333:Central Nervous System Sensitization / Q000187:drug effects; D004198:Disease Susceptibility; D005260:Female; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D009020:Morphine / Q000633:toxicity; D009021:Morphine Dependence / Q000209:etiology* / Q000378:metabolism; D009294:Narcotics / Q000633:toxicity; D009419:Nerve Tissue Proteins / Q000819:agonists / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D043884:Receptor, Cannabinoid, CB1 / Q000819:agonists / Q000378:metabolism; D043885:Receptor, Cannabinoid, CB2 / Q000819:agonists / Q000378:metabolism; D013037:Spatial Behavior / Q000187:drug effects*
chemical_list: D063386:Cannabinoid Receptor Agonists; D002186:Cannabinoids; C543264:Cnr1 protein, rat; C469526:Cnr2 protein, rat; D009294:Narcotics; D009419:Nerve Tissue Proteins; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2; D009020:Morphine