title: Immunomodulatory activity of vardenafil on induced lung inflammation in cystic fibrosis mice.
abstract: BACKGROUND
  We tested the hypothesis that vardenafil, a common drug used for improving erectile dysfunction and able to partially normalize transepithelial chloride transport in cystic fibrosis (CF), modulates CF lung inflammation.
  
  
  METHODS
  Inflammatory markers in lungs of F508del-CF and wild-type mice were monitored in response to lipopolysaccharide from Pseudomonas aeruginosa (LPS). The effect of pretreatment with vardenafil (0.14 mg/kg) was evaluated.
  
  
  RESULTS
  A latent inflammatory status, characterized by neutrophil infiltrate, mouse macrophage inflammatory protein (MIP)-2 and tumor necrosis factor (TNF)-α, was found in baseline conditions in F508del-CF mice. Inflammatory markers were increased after LPS with higher responses in CF. Vardenafil globally attenuated inflammatory responses in both genotypes however reduction of macrophage infiltration, macrophage chemoattractant chemokine and interleukin-1β was observed in the CF group only.
  
  
  CONCLUSIONS
  Vardenafil reduces lung inflammation with a more pronounced effect in F508del-CF mice, particularly on macrophage cell markers.
authors.affiliation: Louvain centre for Toxicology and Applied Pharmacology, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Bob
authors.initials: B
authors.lastname: Lubamba
authors.forename: François
authors.initials: F
authors.lastname: Huaux
authors.forename: Jean
authors.initials: J
authors.lastname: Lebacq
authors.forename: Etienne
authors.initials: E
authors.lastname: Marbaix
authors.forename: Barbara
authors.initials: B
authors.lastname: Dhooghe
authors.forename: Nadtha
authors.initials: N
authors.lastname: Panin
authors.forename: Pierre
authors.initials: P
authors.lastname: Wallemacq
authors.forename: Teresinha
authors.initials: T
authors.lastname: Leal
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D003550:Cystic Fibrosis / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D007093:Imidazoles / Q000494:pharmacology*; D056747:Immunomodulation / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D018181:Mice, Inbred CFTR; D058986:Phosphodiesterase 5 Inhibitors / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology*; D011014:Pneumonia / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology*; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000276:immunology; D013450:Sulfones / Q000494:pharmacology; D014227:Triazines / Q000494:pharmacology; D000069058:Vardenafil Dihydrochloride
chemical_list: D007093:Imidazoles; D008070:Lipopolysaccharides; D058986:Phosphodiesterase 5 Inhibitors; D010879:Piperazines; D013450:Sulfones; D014227:Triazines; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D000069058:Vardenafil Dihydrochloride