title: The number of Toll-like receptor 9-agonist motifs in the adenovirus genome correlates with induction of dendritic cell maturation by adenovirus immune complexes.
abstract: Adenovirus serotype 5 (Ad5) vectors and specific neutralizing antibodies (NAbs) generate immune complexes (ICs) which are potent inducers of dendritic cell (DC) maturation. Here we show that ICs generated with rare Ad vector serotypes, such as Ad26 and Ad35, which are lead candidates in HIV vaccine development, are poor inducers of DC maturation and that their potency in inducing DC maturation strongly correlated with the number of Toll-like receptor 9 (TLR9)-agonist motifs present in the Ad vector's genome. In addition, we showed that antihexon but not antifiber antibodies are responsible for the induction of Ad IC-mediated DC maturation.
authors.affiliation: Division of Immunology and Allergy, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland. Matthieu.Perreau@chuv.ch
authors.forename: Matthieu
authors.initials: M
authors.lastname: Perreau
authors.forename: Hugh C
authors.initials: HC
authors.lastname: Welles
authors.forename: Celine
authors.initials: C
authors.lastname: Pellaton
authors.forename: Bebeka
authors.initials: B
authors.lastname: Gjoksi
authors.forename: Lambert
authors.initials: L
authors.lastname: Potin
authors.forename: Ricardo
authors.initials: R
authors.lastname: Martin
authors.forename: Alexandre
authors.initials: A
authors.lastname: Harari
authors.forename: Andrew
authors.initials: A
authors.lastname: Bett
authors.forename: Danilo
authors.initials: D
authors.lastname: Casimiro
authors.forename: Jason
authors.initials: J
authors.lastname: Gall
authors.forename: Dan H
authors.initials: DH
authors.lastname: Barouch
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Kremer
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pantaleo
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics* / Q000276:immunology*; D020816:Amino Acid Motifs; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000936:Antigen-Antibody Complex; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology*; D006801:Humans; D051217:Toll-Like Receptor 9 / Q000819:agonists* / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D051217:Toll-Like Receptor 9; D014764:Viral Proteins