title: Effects of ebselen on radiocontrast media-induced hepatotoxicity in rats.
abstract: Oxidative stress is accepted as a potential responsible mechanism in the pathogenesis of radiocontrast media (RCM)-induced hepatotoxicity. Therefore, we aimed to investigate the protective effects of ebselen against RCM-induced hepatotoxicity by measuring tissue oxidant/antioxidant parameters and histological changes in rats. Wistar albino rats were randomly separated into four groups consisting of eight rats per group. Normal saline was given to the rats in control group (group 1). RCM was given to the rats in group 2, and both RCM and ebselen were given to the rats in group 3. Only ebselen was given to the rats in group 4. Liver sections of the killed animals were analyzed to measure the levels of malondialdehyde (MDA) and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), as well as histopathological changes. In RCM group, SOD and CAT levels were found increased. In RCM-ebselen group, MDA, SOD and CAT levels were found decreased. In RCM-ebselen group, however, GSH-Px activities of liver tissue increased. All these results indicated that ebselen produced a protective mechanism against RCM-induced hepatotoxicity and took part in oxidative stress.
authors.affiliation: Department of Pediatrics, Mustafa Kemal University, Hatay, Turkey. fatmagulbasarslan@hotmail.com
authors.forename: Fatmagul
authors.initials: F
authors.lastname: Basarslan
authors.forename: Nigar
authors.initials: N
authors.lastname: Yilmaz
authors.forename: Isil
authors.initials: I
authors.lastname: Davarci
authors.forename: Mustafa
authors.initials: M
authors.lastname: Akin
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozgur
authors.forename: Cahide
authors.initials: C
authors.lastname: Yilmaz
authors.forename: Kemal Turker
authors.initials: KT
authors.lastname: Ulutas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001393:Azoles / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D003287:Contrast Media / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000378:metabolism; D054833:Isoindoles; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D016566:Organoselenium Compounds / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001393:Azoles; D003287:Contrast Media; D054833:Isoindoles; D016566:Organoselenium Compounds; C042986:ebselen; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase