title: Microdeletion 19p13.2 in an almost 5-year-old boy.
abstract: Deletions of the short arm of chromosome 19 are rarely found by conventional cytogenetic techniques. This region has a high gene density and this is likely the reason why deletions in this region are associated with a severe phenotype. Since the implementation of modern high-resolution SNP- and CGH-array techniques more cases have been reported. Here, we present an almost 5-year-old boy with intellectual disability, minor dysmorphisms, febrile seizures, and a de novo deletion of 834.2 kb on 19p13.2 encompassing 32 genes. The deletion was found by the Illumina Infinium HD Human1M-Duo v1 BeadChip SNP-array and confirmed by the NimbleGen Human CGH 2.1M Whole Genome Tiling v2.0D oligonucleotide array. PCR amplification of the junction fragment and subsequent sequencing defined the breakpoints and indicated that formation was mediated by non-allelic homologous recombination (NAHR). The phenotype of our patient shows that microrearrangements even at gene-dense chromosomes may result in mild clinical consequences.
authors.affiliation: Clinical Department of Pediatrics IV, Innsbruck Medical University, Innsbruck, Austria.
authors.forename: Edda
authors.initials: E
authors.lastname: Haberlandt
authors.forename: Ana
authors.initials: A
authors.lastname: Spreiz
authors.forename: Sara Baumgartner
authors.initials: SB
authors.lastname: Sigl
authors.forename: Carolin
authors.initials: C
authors.lastname: Janetschek
authors.forename: Benno
authors.initials: B
authors.lastname: Röthlisberger
authors.forename: Sibylle
authors.initials: S
authors.lastname: Zotter
authors.forename: Kevin
authors.initials: K
authors.lastname: Rostasy
authors.forename: Johannes
authors.initials: J
authors.lastname: Zschocke
authors.forename: Dieter
authors.initials: D
authors.lastname: Kotzot
mesh_terms: D002675:Child, Preschool; D002872:Chromosome Deletion; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D023281:Genomics / Q000379:methods; D059765:Homologous Recombination; D006801:Humans; D008607:Intellectual Disability; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012640:Seizures