title: Hematopoietic progenitor cells and restenosis after carotid endarterectomy.
abstract: OBJECTIVE
  Hematopoietic progenitor cells (HPCs) may attenuate the response to vascular injury by maintaining endothelial integrity and function. Our aim was to determine whether circulating HPC number and function correlate with restenosis after carotid endarterectomy.
  
  
  METHODS
  HPC number (CD34(+)/CD133(+) cells), early colony-forming units, migratory capacity, and senescence were analyzed in blood collected preoperatively, 1 day, and 6 weeks postoperatively. Mobilizing cytokine levels were also measured. Stenosis was assessed by duplex scanning.
  
  
  RESULTS
  HPC numbers (P<0.001) and early colony-forming unit count (P=0.001) fell rapidly 24 hours postoperatively. Restenosis at 6 months correlated negatively with the magnitude of postoperative falls in HPC numbers (R=-0.38, P=0.013) and early colony-forming unit counts (R=-0.42, P=0.008). The migratory capacity of preoperative HPCs correlated negatively with restenosis (R=-0.48, P=0.007). Preoperative SDF1 levels correlated with falls in HPC number (R=0.42, P=0.044) and early colony-forming unit counts (R=0.56, P=0.004).
  
  
  CONCLUSIONS
  HPC function appears to be linked to the development of carotid artery restenosis after endarterectomy. These data support the concept that HPCs have a role in regulating remodeling of the injured arterial wall.
authors.affiliation: Kings College London, BHF Centre of Research Excellence, Academic Department of Surgery, Cardiovascular Division, St Thomas’ Hospital, London, UK.
authors.forename: Sanjay D
authors.initials: SD
authors.lastname: Patel
authors.forename: Julia
authors.initials: J
authors.lastname: Humphries
authors.forename: Katherine
authors.initials: K
authors.lastname: Mattock
authors.forename: Ashar
authors.initials: A
authors.lastname: Wadoodi
authors.forename: Bijan
authors.initials: B
authors.lastname: Modarai
authors.forename: Anwar
authors.initials: A
authors.lastname: Ahmad
authors.forename: Kevin G
authors.initials: KG
authors.lastname: Burnand
authors.forename: Matthew
authors.initials: M
authors.lastname: Waltham
authors.forename: Alberto
authors.initials: A
authors.lastname: Smith
mesh_terms: D000071916:AC133 Antigen; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000097:blood; D018952:Antigens, CD34 / Q000097:blood; D016893:Carotid Stenosis / Q000097:blood* / Q000473:pathology / Q000601:surgery; D054377:Chemokine CXCL12 / Q000097:blood*; D016894:Endarterectomy, Carotid; D004730:Endothelium, Vascular / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006023:Glycoproteins / Q000097:blood; D006412:Hematopoietic Stem Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000097:blood; D012038:Regeneration
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides