title: Expression of functional full-length hSRC-1 in eukaryotic cells using modified vaccinia virus Ankara and baculovirus.
abstract: Purified protein expression level and quality are contingent upon specific host expression systems. This differential production is particularly observed for proteins of high molecular weight, hampering further structural studies. We developed an expression method aimed at producing proteins in Escherichia coli, insect, and mammalian systems. Our novel protocol was used to produce in large scale the full-length 160-kDa steroid receptor coactivator 1 (SRC-1), a coregulator of nuclear receptors. The results indicate that we can produce biologically active human SRC-1 in mammalian and insect cells in large scale.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, Institut National de Santé et de Recherche Médicale U964/Centre National de Recherche Scientifique UMR 7104/Université de Strasbourg, 67404 Illkirch, France.
authors.forename: Judit
authors.initials: J
authors.lastname: Osz
authors.forename: Karine
authors.initials: K
authors.lastname: Pradeau-Aubreton
authors.forename: Robert
authors.initials: R
authors.lastname: Drillien
authors.forename: Nathalie
authors.initials: N
authors.lastname: Troffer-Charlier
authors.forename: Isabelle
authors.initials: I
authors.lastname: Kolb-Cheynel
authors.forename: Arnaud
authors.initials: A
authors.lastname: Poterszman
authors.forename: Marc
authors.initials: M
authors.lastname: Ruff
authors.forename: Dino
authors.initials: D
authors.lastname: Moras
authors.forename: Natacha
authors.initials: N
authors.lastname: Rochel
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D006224:Cricetinae; D005822:Genetic Vectors / Q000378:metabolism*; D006801:Humans; D056920:Nuclear Receptor Coactivator 1 / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D056920:Nuclear Receptor Coactivator 1