title: Chemical reporters for monitoring RNA synthesis and poly(A) tail dynamics.
abstract: A versatile "clickable" nucleoside: Metabolic labeling of cells is useful in studying the dynamics of biological molecules. N(6) pA can be utilized by all three mammalian RNA polymerases, as well as poly(A) polymerase. Because of its alkyne modification, RNA labeled with N(6) pA can be visualized and purified by using click chemistry.
authors.affiliation: Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, 1230 York Avenue Box 250, New York, NY 10065, USA.
authors.forename: Markus
authors.initials: M
authors.lastname: Grammel
authors.forename: Howard
authors.initials: H
authors.lastname: Hang
authors.forename: Nicholas K
authors.initials: NK
authors.lastname: Conrad
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D057930:Click Chemistry; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D011061:Poly A / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D000241:Adenosine