title: Salt-induced chloroplast protrusion is the process of exclusion of ribulose-1,5-bisphosphate carboxylase/oxygenase from chloroplasts into cytoplasm in leaves of rice.
abstract: Chloroplast protrusions (CPs) are often observed under environmental stresses, but their role has not been elucidated. The formation of CPs was observed in the leaf of rice plants treated with 75 mm NaCl for 14 d. Some CPs were almost separated from the main chloroplast body. In some CPs, inner membrane structures and crystalline inclusions were included. Similar structures surrounded by double membranes were observed in the cytoplasm and vacuole. Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) was detected in CPs and the similar structures in the cytoplasm and vacuole. These results suggest that CP is one of the pathways of Rubisco exclusion from chloroplasts into the cytoplasm under salinity, and the exclusions could be transported to vacuole for their degradation.
authors.affiliation: School of Agriculture, Kinki University, Nara 631-8505 Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yamane
authors.forename: Shiro
authors.initials: S
authors.lastname: Mitsuya
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyake
mesh_terms: D002736:Chloroplasts / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D058503:Mesophyll Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D008954:Models, Biological; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D021381:Protein Transport / Q000187:drug effects; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology*; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D012965:Sodium Chloride; D012273:Ribulose-Bisphosphate Carboxylase