title: Association between the levels of circulating adhesion molecules and biopterins in type-2 diabetic normotensive patients adhesion molecules and biopterins.
abstract: Endothelial dysfunction is a common feature in type-2 diabetic patients and is associated with inflammation, increased levels of circulating soluble adhesion molecules and atherosclerosis. Insufficiency of tetrahydrobiopterin leads to uncoupling of the nitric oxide synthase enzyme an endothelial dysfunction.
  
  
  OBJECTIVE
  was to evaluate if there is a relationship between the levels of circulating soluble adhesion molecules and the levels of biopterins in normotensive type-2 diabetic patients.
  
  
  METHODS
  We studied 30 normotensive type-2 diabetic patients in whom VCAM-1, ICAM-1 and E-selectin were measured by ELISA. Additionally, Biopterins were measured by reverse phase high performance liquid chromatography with fluorescence detection. The levels of circulating adhesion molecules and biopterins were correlated using the Spearman correlation coefficient test. Statistical analysis was performed with ANOVA.
  
  
  RESULTS
  We did not find any relationship between absolute values of biopterins and soluble adhesion molecules. However, we observed significant inverse correlations between the BH4/BH2 ratio and VCAM-1 (r= -0.65, p<0.001) with ICAM-1 (r= -0.69, p<0.001) and with E-selectin (r=-0.64 p<0.001),
  
  
  CONCLUSIONS
  Our data suggest that systemic levels of adhesion molecules have an inverse association with the BH4/BH2 ratio in type 2 diabetic normotensive patients.
authors.affiliation: Metabolic and Research Clinic, Hospital General de Ticomán SS DF, México DF, Mexico. clinhta@hotmail.com
authors.forename: Alberto Francisco
authors.initials: AF
authors.lastname: Rubio-Guerra
authors.forename: Hilda
authors.initials: H
authors.lastname: Vargas-Robles
authors.forename: Leonardo
authors.initials: L
authors.lastname: Del Valle-Mondragon
authors.forename: Jose Juan
authors.initials: JJ
authors.lastname: Lozano-Nuevo
authors.forename: Bruno Alfonso
authors.initials: BA
authors.lastname: Escalante-Acosta
mesh_terms: D000368:Aged; D001708:Biopterins / Q000031:analogs & derivatives* / Q000097:blood; D001794:Blood Pressure; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000276:immunology / Q000503:physiopathology; D019040:E-Selectin / Q000097:blood* / Q000737:chemistry; D004730:Endothelium, Vascular / Q000276:immunology / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D012995:Solubility; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood* / Q000737:chemistry
chemical_list: D019040:E-Selectin; C573420:ICAM1 protein, human; C546936:SELE protein, human; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D001708:Biopterins; C017226:7,8-dihydrobiopterin; C003402:sapropterin