title: Extracellular matrix composition in COPD.
abstract: Extracellular matrix (ECM) composition has an important role in determining airway structure. We postulated that ECM lung composition of chronic obstructive pulmonary disease (COPD) patients differs from that observed in smoking and nonsmoking subjects without airflow obstruction. We determined the fractional areas of elastic fibres, type-I, -III and -IV collagen, versican, decorin, biglycan, lumican, fibronectin and tenascin in different compartments of the large and small airways and lung parenchyma in 26 COPD patients, 26 smokers without COPD and 16 nonsmoking control subjects. The fractional area of elastic fibres was higher in non-obstructed smokers than in COPD and nonsmoking controls, in all lung compartments. Type-I collagen fractional area was lower in the large and small airways of COPD patients and in the small airways of non-obstructed smokers than in nonsmokers. Compared with nonsmokers, COPD patients had lower versican fractional area in the parenchyma, higher fibronectin fractional area in small airways and higher tenascin fractional area in large and small airways compartments. In COPD patients, significant correlations were found between elastic fibres and fibronectin and lung function parameters. Alterations of the major ECM components are widespread in all lung compartments of patients with COPD and may contribute to persistent airflow obstruction.
authors.affiliation: Dept of Pathology, University of São Paulo Medical School, São Paulo, Brazil. rqannoni@yahoo.com.br
authors.forename: Raquel
authors.initials: R
authors.lastname: Annoni
authors.forename: Tatiana
authors.initials: T
authors.lastname: Lanças
authors.forename: Ryan
authors.initials: R
authors.lastname: Yukimatsu Tanigawa
authors.forename: Marcus
authors.initials: M
authors.lastname: de Medeiros Matsushita
authors.forename: Sandra
authors.initials: S
authors.lastname: de Morais Fernezlian
authors.forename: Andreina
authors.initials: A
authors.lastname: Bruno
authors.forename: Luiz
authors.initials: L
authors.lastname: Fernando Ferraz da Silva
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Roughley
authors.forename: Salvatore
authors.initials: S
authors.lastname: Battaglia
authors.forename: Marisa
authors.initials: M
authors.lastname: Dolhnikoff
authors.forename: Pieter S
authors.initials: PS
authors.lastname: Hiemstra
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Sterk
authors.forename: Klaus F
authors.initials: KF
authors.lastname: Rabe
authors.forename: Thais
authors.initials: T
authors.lastname: Mauad
mesh_terms: D000328:Adult; D000368:Aged; D058578:Biglycan / Q000378:metabolism; D016022:Case-Control Studies; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D058575:Decorin / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007632:Keratan Sulfate / Q000378:metabolism; D000071234:Lumican; D008168:Lung / Q000378:metabolism / Q000601:surgery; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism*; D012129:Respiratory Function Tests; D012907:Smoking / Q000009:adverse effects; D019063:Tenascin / Q000378:metabolism
chemical_list: D058578:Biglycan; D011508:Chondroitin Sulfate Proteoglycans; D058575:Decorin; D005353:Fibronectins; C000604306:LUM protein, human; D000071234:Lumican; D019063:Tenascin; D003094:Collagen; D007632:Keratan Sulfate