title: DNA hybridization assay at individual, biofunctionalized zinc oxide nanowires.
abstract: Reliable and efficient identification of DNA is a major goal in on-site diagnostics. One dimensional nanostructures like nanowires (NW) represent potential sensor structures due to their extreme surface-to-bulk ratio, enabling enhanced biomolecule binding which results in optimal signals. While silicon NW are already well studied, NW made from other materials with promising properties like ZnO are not yet established as NW sensor material for bioanalytics. Here we demonstrate the DNA functionalization of ZnO NW even at the single NW level and their successful application in a DNA hybridization assay.
authors.affiliation: Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany. christian.leiterer@gmail.com
authors.forename: Christian
authors.initials: C
authors.lastname: Leiterer
authors.forename: Barbara
authors.initials: B
authors.lastname: Seise
authors.forename: Irma
authors.initials: I
authors.lastname: Slowik
authors.forename: Gerald
authors.initials: G
authors.lastname: Brönstrup
authors.forename: Raphael
authors.initials: R
authors.lastname: Niepelt
authors.forename: Karina
authors.initials: K
authors.lastname: Weber
authors.forename: Carsten
authors.initials: C
authors.lastname: Ronning
authors.forename: Silke
authors.initials: S
authors.lastname: Christiansen
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fritzsche
mesh_terms: D002232:Carbocyanines; D004247:DNA / Q000302:isolation & purification*; D005456:Fluorescent Dyes; D061925:Immobilized Nucleic Acids; D036103:Nanotechnology; D053770:Nanowires / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D055620:Optical Phenomena; D012825:Silicon; D014159:Transducers; D015034:Zinc Oxide
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D061925:Immobilized Nucleic Acids; C089187:cyanine dye 3; D004247:DNA; D015034:Zinc Oxide; D012825:Silicon