title: Silencing of FABP3 promotes apoptosis and induces mitochondrion impairment in embryonic carcinoma cells.
abstract: Fatty acid binding protein 3 (FABP3) (also known as H-FABP) is a member of the intracellular lipid-binding protein family, and is mainly expressed in cardiac muscle tissue. The in vivo function of FABP3 is proposed to be in fatty acid metabolism, trafficking, and cell signaling. Our previous study found that FABP3 is highly regulated in patients with ventricular septal defect (VSD), and may play a significant role in the development of human VSD. In the present study, we aimed to investigate the impact of FABP3 knockdown by RNA interference (RNAi) on apoptosis and mitochondrial function of embryonic carcinoma (P19) cells. The results revealed that downregulated FABP3 expression promoted apoptosis, and resulted in mitochondrial deformation, increased mitochondrial membrane potential (MMP), and decreased intracellular ATP synthesis. In addition, the knockdown of FABP3 also led to excess intracellular ROS production. However, there was no obvious influence on the amount of mitochondrial DNA. Collectively, our results indicated that FABP3 knockdown promoted apoptosis and caused mitochondrial dysfunction in P19 cells, which might be responsible for the development of human VSD.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, People's Republic of China.
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Shen
authors.forename: Gui-Xian
authors.initials: GX
authors.lastname: Song
authors.forename: Yao-Qiu
authors.initials: YQ
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhou
authors.forename: Hai-Lang
authors.initials: HL
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Yang
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Sheng
authors.forename: Ling-Mei
authors.initials: LM
authors.lastname: Qian
authors.forename: Xiang-Qing
authors.initials: XQ
authors.lastname: Kong
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D004272:DNA, Mitochondrial / Q000235:genetics; D054278:Embryonal Carcinoma Stem Cells / Q000378:metabolism* / Q000473:pathology*; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D018628:Gene Dosage; D055785:Gene Knockdown Techniques; D051379:Mice; D008854:Microscopy, Electron; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection
chemical_list: D004272:DNA, Mitochondrial; C493409:Fabp3 protein, mouse; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate