title: Quantitative measurement of optical parameters of cell lines 5-8F and 6-10B using polarization sensitive optical coherence tomography.
abstract: The aim was to test whether the typical NPC cell lines of 5-8F (high tumorigenesis and metastasis) and 6-10B (low tumorigenesis and metastasis) could be differentiated by polarization sensitive optical coherence tomography (PS-OCT). We imaged the two types of low cellular differentiated NPC cell lines 5-8F and 6-10B pellets using PS-OCT; then extracted the optical parameters of attenuate coefficient and anisotropy from the A-scan lines based on the multiple scattering model; and compared their phase retardation. The fitting scattering coefficients were μs=10.91±0.45 and μs=11.33±0.27 cm(-1) for 5-8F and 6-10B pellets (p<0.05), respectively; and the anisotropy factors were g=0.900±0.013 and g=0.885±0.008 for 5-8F and 6-10B pellets (p<0.01), respectively. While the phase retardation of 6-10B was a little faster than 5-8F. These results indicated that PS-OCT could differentiate the two cell lines, and had the potential ability for typing the tissue of NPC.
authors.affiliation: School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Li
authors.forename: Bingling
authors.initials: B
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Du
authors.forename: Lifeng
authors.initials: L
authors.lastname: Yang
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Xia
authors.forename: Yonghong
authors.initials: Y
authors.lastname: He
authors.forename: Songhao
authors.initials: S
authors.lastname: Liu
authors.forename: Changshui
authors.initials: C
authors.lastname: Chen
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000473:pathology*; D041623:Tomography, Optical Coherence / Q000379:methods*