title: Effect of Coffea canephora aqueous extract on microbial counts in ex vivo oral biofilms: a case study.
abstract: In the present study, the ex vivo antimicrobial effect of brewed coffee was tested on oral biofilms. For this, unsweetened and sweetened (10 % sucrose) brewed light-roasted Coffea canephora at 20 % was used in biofilms formed by non-stimulated saliva from three volunteers. After 30 min contact with unsweetened and sweetened brews, the average microorganism count in the biofilms reduced by 15.2 % and 12.4 %, respectively, with no statistical difference among them. We also observed a drop of microorganisms in the biofilms after treatment with sucrose solution at 5 % compared to control (saline) and to sucrose at 1 % and 3 %. In conclusion, Coffea canephora extract reduces the microbial count in oral biofilm, and our data suggest that sucrose concentration in coffee brew can influence its antimicrobial property against the referred biofilm.
authors.affiliation: Departamento de Odontopediatria e Ortodontia, Faculdade de Odontologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. andreagantonio@yahoo.com.br
authors.forename: Andréa Gonçalves
authors.initials: AG
authors.lastname: Antonio
authors.forename: Natália Lopes Pontes
authors.initials: NL
authors.lastname: Iorio
authors.forename: Adriana
authors.initials: A
authors.lastname: Farah
authors.forename: Kátia Regina
authors.initials: KR
authors.lastname: Netto dos Santos
authors.forename: Lucianne Cople
authors.initials: LC
authors.lastname: Maia
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis* / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D002710:Chlorhexidine / Q000031:analogs & derivatives; D040503:Coffea / Q000737:chemistry*; D003069:Coffee / Q000737:chemistry*; D015169:Colony Count, Microbial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology*; D013395:Sucrose
chemical_list: D000890:Anti-Infective Agents; D003069:Coffee; D013395:Sucrose; C010882:chlorhexidine gluconate; D002710:Chlorhexidine