title: Effect of surgical and chemical sensory denervation on non-neural expression of the transient receptor potential vanilloid 1 (TRPV1) receptors in the rat.
abstract: Pretreatment with the ultrapotent capsaicin analog resiniferatoxin (RTX) has been applied as a selective pharmacological tool in inflammation and pain studies to desensitize transient receptor potential vanilloid 1 (TRPV1) receptor-expressing sensory nerve endings. The discovery of TRPV1 receptor on non-neural cells challenges systemic RTX desensitization as a method acting exclusively on a population of sensory neurons, but not on non-neural cells. Systemic RTX desensitization was used for chemical denervation and transection of the sciatic and saphenous nerves for surgical denervation in rats. Quantitative real-time PCR and immunohistochemistry were applied to investigate the presence and alterations of the TRPV1 receptor mRNA and protein following chemical and surgical denervation. We provided the first evidence for non-neural TRPV1 immunopositivity and mRNA expression in the rat dorsal paw and plantar skin as well as the oral mucosa. Neither chemical nor surgical denervation influenced the level of TRPV1 receptor mRNA and protein expression in non-neural cells of either skin regions or mucosa. Therefore, RTX and consequently capsaicin remain to be considered as selective neurotoxins for a population of primary afferent neurons.
authors.affiliation: Department of Pharmacology and Pharmacotherapy, University of Pécs, Szigeti 12., 7624 Pécs, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Kun
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Helyes
authors.forename: Anikó
authors.initials: A
authors.lastname: Perkecz
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Bán
authors.forename: Beáta
authors.initials: B
authors.lastname: Polgár
authors.forename: János
authors.initials: J
authors.lastname: Szolcsányi
authors.forename: Erika
authors.initials: E
authors.lastname: Pintér
mesh_terms: D000700:Analgesics / Q000633:toxicity; D000818:Animals; D019771:Axotomy; D003714:Denervation; D004224:Diterpenes / Q000633:toxicity; D005267:Femoral Nerve / Q000293:injuries; D005528:Foot; D005786:Gene Expression Regulation / Q000502:physiology*; D006614:Hindlimb; D008297:Male; D008856:Microscopy, Fluorescence; D009061:Mouth Mucosa / Q000378:metabolism*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D012584:Sciatic Nerve / Q000293:injuries; D012867:Skin / Q000378:metabolism*; D013563:Sympathectomy, Chemical; D050916:TRPV Cation Channels / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000700:Analgesics; D004224:Diterpenes; D012333:RNA, Messenger; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; C024353:resiniferatoxin