title: A new type of Kazal proteinase inhibitor related to shrimp Penaeus (Litopenaeus) vannamei immunity.
abstract: A clone encoding a four-Kazal domain-containing protein was isolated from the hemostats of a Penaeus vannamei cDNA library. The full-length cDNA sequence is 975 bp in length and encodes a 24.4 kDa protein (228 residues). Four Kazal domains, each 43-46 residues in length, were detected in the deduced primary structure. The first, third and fourth domains have the CPLREELPVC, CPAVYDPVC and CPLYVDPVC motifs, respectively, suggesting that they are able to inhibit chymotrypsin and elastase. The mRNA levels of the Kazal protein were modified after the injection of Vibrio alginolyticus, indicating the probable role of this protein in the immune response. All these characteristics are similar to previously reported shrimp Kazal, however, based on both domain architecture and expression profile following Vibrio stimulation, this protein represents a new type of Kazal inhibitor associated with shrimp immunity.
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo (CIAD), Marine Biotechnology Lab, PO Box 1735, Hermosillo, Son 83000, Mexico. fvalbores@ciad.mx
authors.forename: Francisco
authors.initials: F
authors.lastname: Vargas-Albores
authors.forename: Enrique
authors.initials: E
authors.lastname: Villalpando
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000201:enzymology / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D010802:Phylogeny; D016415:Sequence Alignment; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D044149:Vibrio alginolyticus / Q000276:immunology
chemical_list: D015842:Serine Proteinase Inhibitors