title: Effect of different methods for decontaminating tooth enamel after contact with blood before bonding orthodontic buttons.
abstract: OBJECTIVE
  To evaluate the best method for decontaminating tooth enamel contaminated by contact with blood before bonding orthodontic buttons.
  
  
  METHODS
  The labial surfaces of 195 bovine incisors initially received prophylaxis, followed by 37% phosphoric acid etching, adhesive application, and light polymerization. After this, the labial surfaces of all teeth were contaminated with blood. The teeth were then randomly divided into 13 groups (n = 15), comprising the control group (treated according to the manufacturer's recommendations) and 12 experimental groups treated by the following decontamination methods: group 1, no decontamination; group 2, washing with distilled water; group 3, washing with physiologic solution; group 4, jets of air; group 5, gauze; group 6, cotton wool; group 7, distilled water plus jets of air; group 8, distilled water plus gauze; group 9, distilled water plus cotton wool; group 10, physiologic solution plus jets of air; group 11, physiologic solution plus gauze; and group 12, physiologic solution plus cotton wool.
  
  
  RESULTS
  No statistical differences were shown between the control group and groups 4, 7, 10, and 11 (P > .05). The lowest bond strength values were shown in group 1, in which no decontamination was performed, and groups 6 and 12, which were decontaminated with cotton wool and physiologic solution plus cotton wool, respectively.
  
  
  CONCLUSIONS
  The best method of decontaminating enamel contaminated with blood is washing with physiologic solution, followed by drying with jets of air and gauze or drying with jets of air only.
authors.affiliation: Southwest Bahia State University, UESB, Bahia, Brazil. matheuspithon@gmail.com
authors.forename: Matheus Melo
authors.initials: MM
authors.lastname: Pithon
authors.forename: Vanessa Oliveira
authors.initials: VO
authors.lastname: Batista
authors.forename: Natalli Cardoso Cafeseiro
authors.initials: NC
authors.lastname: D'el Rey
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000388:Air; D000818:Animals; D001688:Biological Products / Q000737:chemistry; D001769:Blood; D002417:Cattle; D032561:Cotton Fiber; D003666:Decontamination / Q000379:methods*; D001840:Dental Bonding; D003743:Dental Enamel / Q000033:anatomy & histology*; D003777:Dental Prophylaxis / Q000295:instrumentation / Q000379:methods; D003799:Dental Stress Analysis / Q000295:instrumentation; D003890:Desiccation; D009967:Orthodontic Appliances; D010756:Phosphoric Acids / Q000737:chemistry; D055668:Photochemical Processes; D058105:Polymerization; D011897:Random Allocation; D019279:Resin Cements / Q000737:chemistry; D012464:Saliva, Artificial / Q000737:chemistry; D033081:Shear Strength; D013314:Stress, Mechanical; D013499:Surface Properties; D015503:Surgical Sponges; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D001688:Biological Products; D010756:Phosphoric Acids; D019279:Resin Cements; D012464:Saliva, Artificial; C477790:Transbond XT; D014867:Water; C030242:phosphoric acid