title: A flow cytometry-based dopamine transporter binding assay using antagonist-conjugated quantum dots.
abstract: Here we present the development and validation of a flow cytometry-based dopamine transporter (DAT) binding assay that uses antagonist-conjugated quantum dots (QDs). We anticipate that our QD-based assay is of immediate value to the high throughput screening of novel DAT modulators.
authors.affiliation: Department of Chemistry, Vanderbilt University, 7300 Stevenson Ctr Ln, Nashville, TN 37235, USA.
authors.forename: Oleg
authors.initials: O
authors.lastname: Kovtun
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Ross
authors.forename: Ian D
authors.initials: ID
authors.lastname: Tomlinson
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: Rosenthal
mesh_terms: D001667:Binding, Competitive; D001710:Biotin / Q000737:chemistry; D004298:Dopamine / Q000378:metabolism; D018492:Dopamine Antagonists / Q000494:pharmacology*; D050483:Dopamine Plasma Membrane Transport Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation / Q000187:drug effects; D057809:HEK293 Cells; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D010703:Phorbol Esters / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence
chemical_list: D018492:Dopamine Antagonists; D050483:Dopamine Plasma Membrane Transport Proteins; D005456:Fluorescent Dyes; D010703:Phorbol Esters; D010879:Piperazines; D011092:Polyethylene Glycols; D001710:Biotin; C032974:12-O-retinoylphorbol-13-acetate; C043425:vanoxerine; D004298:Dopamine