title: Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli.
abstract: Fluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis, Enterococcus faecalis, and Escherichia coli.
authors.affiliation: Departamento de Biotecnología y Microbiología de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM), CEI UAM+CSIC, Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Tomás
authors.initials: T
authors.lastname: García-Cayuela
authors.forename: Luz P Gómez
authors.initials: LP
authors.lastname: de Cadiñanos
authors.forename: M Luz
authors.initials: ML
authors.lastname: Mohedano
authors.forename: Pilar Fernández
authors.initials: PF
authors.lastname: de Palencia
authors.forename: Daniel
authors.initials: D
authors.lastname: Boden
authors.forename: Jerry
authors.initials: J
authors.lastname: Wells
authors.forename: Carmen
authors.initials: C
authors.lastname: Peláez
authors.forename: Paloma
authors.initials: P
authors.lastname: López
authors.forename: Teresa
authors.initials: T
authors.lastname: Requena
mesh_terms: D019677:Artificial Gene Fusion; D013293:Enterococcus faecalis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D017930:Genes, Reporter; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D013294:Lactococcus lactis / Q000235:genetics*; D008164:Luminescent Proteins / Q000032:analysis* / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D008164:Luminescent Proteins