title: Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with American cutaneous leishmaniasis.
abstract: BACKGROUND
  The study analyzed positivity of polymerase chain reaction (PCR) on detection of DNA from Leishmania in patients' samples.
  
  
  METHODS
  Extracted DNA was submitted to L150/L152, 13Y/13Z, and seminested PCR (snPCR).
  
  
  RESULTS
  Results were evidenced by bands of approximately 120, 720, and 670 bp for L150/L152, 13Y/13Z, and snPCR, respectively. L150/L152, 13Y/13Z, and snPCR positivity indexes were 76.9, 56.4, and 9.2 (p>0.05), respectively, for suspected and 93.7, 68.7, and 84.4 (p<0.05), respectively, for confirmed.
  
  
  CONCLUSIONS
  Preliminary results showed that these assays, mainly L150/L152 and snPCR, can detect Leishmania DNA and carry potential on laboratory diagnosis of leishmaniasis.
authors.affiliation: Laboratório de Biologia Molecular de Microorganismos, Universidade Federal de Alfenas, Alfenas, MG. oniloaoj@gmail.com
authors.forename: João Guilherme Lino da
authors.initials: JG
authors.lastname: Silva
authors.forename: Thiago Miranda da
authors.initials: TM
authors.lastname: Silva
authors.forename: Eduardo de Figueiredo
authors.initials: Ede F
authors.lastname: Peloso
authors.forename: George Luiz Lins
authors.initials: GL
authors.lastname: Machado-Coelho
authors.forename: Wilson
authors.initials: W
authors.lastname: Mayrink
authors.forename: Marília Caixeta Franco
authors.initials: MC
authors.lastname: Ariosa
authors.forename: Paulo Márcio de Faria e
authors.initials: PM
authors.lastname: Silva
authors.forename: Marcos José
authors.initials: MJ
authors.lastname: Marques
mesh_terms: D016054:DNA, Protozoan / Q000032:analysis*; D006801:Humans; D007891:Leishmania / Q000235:genetics* / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D016054:DNA, Protozoan