title: Capillary zone electrophoresis-based cytotoxicity analysis of Caco-2 cells.
abstract: A capillary zone electrophoresis-based method to evaluate the cytotoxicity of substances to Caco-2 cells was established. The estimation of the injected cell number (500-5000) and the minor effect of injection condition on cytotoxicity determination were investigated. Caco-2 cells the best model of the intestinal absorptive epithelium, were treated with substances and then stained with Trypan Blue and fixed with paraformaldehyde. The treated Caco-2 cells were detected simultaneously at 590 nm and 214 nm, and the absorbance ratio of the two wavelengths (R(590/214)) can reflect simultaneously the loss of cell membrane integrity and the degradation/leak of intracellular components and indicate the cytotoxicity of substances. The cytotoxicity of the four substances sodium sulfite (Na(2)SO(3)), methyl mercury (MeHg), paclitaxel (PTX), and cadmium chloride (CdCl(2)) were determined and compared. There was no obvious cytotoxicity caused by 20 μM Na(2)SO(3) for 24 h treatment, and the toxicity of the other three toxicants was sequenced as: CdCl(2) > MeHg > PTX. The results are in good agreement with the references and the conventional Trypan Blue exclusion counting assay.
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Qu
authors.forename: Meiling
authors.initials: M
authors.lastname: Hu
authors.forename: Jinmei
authors.initials: J
authors.lastname: Ding
authors.forename: Beilei
authors.initials: B
authors.lastname: Lou
mesh_terms: D018938:Caco-2 Cells / Q000187:drug effects*; D019256:Cadmium Chloride / Q000633:toxicity; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008767:Methylmercury Compounds / Q000633:toxicity; D017239:Paclitaxel / Q000633:toxicity; D013447:Sulfites / Q000494:pharmacology; D018675:Toxicity Tests / Q000379:methods*; D014343:Trypan Blue
chemical_list: D008767:Methylmercury Compounds; D013447:Sulfites; D014343:Trypan Blue; D019256:Cadmium Chloride; D017239:Paclitaxel; C025026:sodium sulfite