title: A sunlight-induced rapid synthesis of silver nanoparticles using sodium salt of N-cholyl amino acids and its antimicrobial applications.
abstract: Aqueous solution containing two additives, silver nitrate (AgNO(3)) and sodium salt of N-cholyl amino acid were irradiated by sunlight for the synthesis of spherical shaped AgNPs without the need for an additional stabilizer or capping agent. Variations of N-cholyl amino acid concentration provided good control over the morphology of the AgNPs, while the carboxylate group of bile salt reduced the Ag(+) ions and the amide group binds strongly to the surface of the NPs. The optical properties, morphology of the AgNPs were characterized by UV-visible, transmission electron microscopy (TEM) and dynamic light scattering (DLS) studies. The interaction of N-cholyl amino acid on the AgNPs surface was studied using cyclic voltammetry and FT-IR techniques. The reduction process was completed within 5 min and the synthesized AgNPs were stable for more than 6 months. The possible mechanism of N-cholyl amino acid on the reduction and stabilization of AgNPs is also discussed. The antimicrobial activity of N-cholyl amino acid capped AgNPs against Escherichia coli, Staphylococcus aureus and Pseudomonas aeroginosa using Mueller Hinton broth and the antifungal activity against Candida albicans, Candida krusei and Candida tropicalis using RPMI broth were determined by MIC studies as per CLSI guidelines.
authors.affiliation: Department of Polymer Science, University of Madras, Maraimalai Campus, Guindy, Chennai 600025, India.
authors.forename: M
authors.initials: M
authors.lastname: Annadhasan
authors.forename: V R
authors.initials: VR
authors.lastname: SankarBabu
authors.forename: R
authors.initials: R
authors.lastname: Naresh
authors.forename: K
authors.initials: K
authors.lastname: Umamaheswari
authors.forename: N
authors.initials: N
authors.lastname: Rajendiran
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D002175:Candida / Q000187:drug effects / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D055664:Electrochemical Techniques / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D008956:Models, Chemical; D015394:Molecular Structure; D010084:Oxidation-Reduction / Q000528:radiation effects; D010316:Particle Size; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D012834:Silver / Q000737:chemistry*; D012835:Silver Nitrate / Q000737:chemistry; D020358:Sodium Cholate / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013472:Sunlight
chemical_list: D000596:Amino Acids; D000890:Anti-Infective Agents; D012834:Silver; D012835:Silver Nitrate; D020358:Sodium Cholate