title: Magnetic field enhanced cell uptake efficiency of magnetic silica mesoporous nanoparticles.
abstract: The advantages of using magnetic mesoporous silica nanoparticles (M-MSNs) in biomedical applications have been widely recognized. However, poor uptake efficiency may hinder the potential of M-MSNs in many applications, such as cell tracking, drug delivery, fluorescence and magnetic resonance imaging. An external magnetic field may improve the cellular uptake efficiency. In this paper, we evaluated the effect of a magnetic field on the uptake of M-MSNs. We found that the internalization of M-MSNs by A549 cancer cells could be accelerated and enhanced by a magnetic field. An endocytosis study indicated that M-MSNs were internalized by A549 cells mainly through an energy-dependent pathway, namely clathrin-induced endocytosis. Transmission electron microscopy showed that M-MSNs were trafficked into lysosomes. With the help of a magnetic field, anticancer drug-loaded M-MSNs induced elevated cancer cell growth inhibition.
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Jixi
authors.initials: J
authors.lastname: Zhang
authors.forename: Weiliang
authors.initials: W
authors.lastname: Xia
authors.forename: Hongchen
authors.initials: H
authors.lastname: Gu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002966:Clathrin / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry; D004705:Endocytosis; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D002966:Clathrin; D004337:Drug Carriers; D012822:Silicon Dioxide