title: Proteomic profiling of Tectona grandis L. leaf.
abstract: Tectona grandis L. (teak) is one of the premier hardwood timbers in the world, ranking at present in the top five tropical hardwood species in terms of worldwide plantation area. Characterization of the proteins present in teak leaves will provide a basis for the development of new tools aimed at assisting tree selection, the monitoring of plant propagation, and the certification of clonal and phenotypic identities. In this paper, we describe the extraction, separation, and identification of leaf proteins from T. grandis using a TCA/acetone protocol, 2DE, and MALDI-TOF. After TCA/acetone protein extraction of leaves, 998 well-resolved spots were detected in Coomassie-stained gels within the 10-114 kDa relative molecular mass (Mr) range at a pH ranging from 3 to 11. A total of 120 spots were digested and subjected to MS. Of these, 100 nonredundant protein species were successfully identified. Functional classification of the identified proteins revealed that proteins involved in photosynthesis, protein translation, and energy production were the most abundant. This work is the first high-throughput attempt to study the T. grandis leaf proteome and represents a stepping stone for further differential expression proteomic studies related to growth, development, biomass production, and culture-associated physiological responses.
authors.affiliation: Instituto de Biotecnología de Las Plantas, Universidad Central Marta Abreu de Las Villas, Santa Clara, Villa Clara, CP, Cuba.
authors.forename: Elisa
authors.initials: E
authors.lastname: Quiala
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Cañal
authors.forename: Roberto
authors.initials: R
authors.lastname: Rodríguez
authors.forename: Norma
authors.initials: N
authors.lastname: Yagüe
authors.forename: Maité
authors.initials: M
authors.lastname: Chávez
authors.forename: Raúl
authors.initials: R
authors.lastname: Barbón
authors.forename: Luis
authors.initials: L
authors.lastname: Valledor
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D019686:Lamiaceae / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics
chemical_list: D010940:Plant Proteins; D020543:Proteome