title: A method to determine the kinetics of multiple proteins in human infants with respiratory distress syndrome.
abstract: We report a method to measure in vivo turnover of four proteins from sequential tracheal aspirates obtained from human newborn infants with respiratory distress syndrome using targeted proteomics. We detected enrichment for all targeted proteins approximately 3 h from the start of infusion of [5,5,5-(2)H(3)] leucine, secretion times that varied from 1.2 to 2.5 h, and half lives that ranged between 10 and 21 h. Complement factor B, a component of the alternative pathway of complement activation, had an approximately twofold-longer half-life than the other three proteins. In addition, the kinetics of mature and carboxy-terminal tryptic peptides from the same protein (surfactant protein B) were not statistically different (p = 0.49).
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, WA 98195-5502, USA. mbereman@uw.edu
authors.forename: Michael S
authors.initials: MS
authors.lastname: Bereman
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Tomazela
authors.forename: Hillary S
authors.initials: HS
authors.lastname: Heins
authors.forename: Manuela
authors.initials: M
authors.lastname: Simonato
authors.forename: Paola E
authors.initials: PE
authors.lastname: Cogo
authors.forename: Aaron
authors.initials: A
authors.lastname: Hamvas
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Patterson
authors.forename: F Sessions
authors.initials: FS
authors.lastname: Cole
authors.forename: Michael J
authors.initials: MJ
authors.lastname: MacCoss
mesh_terms: D000595:Amino Acid Sequence; D011415:Complement Factor B / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000032:analysis / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000378:metabolism*; D011510:Proteolipids / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D037701:Pulmonary Surfactant-Associated Protein B / Q000032:analysis / Q000378:metabolism; D012127:Respiratory Distress Syndrome, Newborn / Q000378:metabolism*; D014132:Trachea / Q000378:metabolism*
chemical_list: D011498:Protein Precursors; D011506:Proteins; D011510:Proteolipids; D037701:Pulmonary Surfactant-Associated Protein B; C105511:surfactant protein B propeptide; D011415:Complement Factor B