title: Pathotypic and molecular characterization of a fowl adenovirus associated with inclusion body hepatitis in Saskatchewan chickens.
abstract: Inclusion body hepatitis (IBH) is one of the major global disease problems, causing significant economic losses to poultry industry of the United States and Canada. The disease is characterized by its sudden onset and high mortalities. Amongst different serotypes of fowl adenoviruses (FAdVs) associated with IBH, serotype 8 of group I FAdV has been isolated from majority of IBH cases. In present studies, we isolated a FAdV from morbid liver of a 17-day-old broiler from a Saskatchewan broiler farm. This newly isolated virus was designated as IBHV(SK). However, based on the sequence analysis of the L1 region of the hexon gene, the IBHV(SK) may be classified as FAdV 8b strain 764. These studies describe for the first time the complete hexon gene sequence of FAdV serotype 8b. Experimental infection of 2-day-old (n = 48) and 2-wk-old (n = 56) chicks caused 83% and 43% mortalities, respectively. Determination of the complete hexon gene sequence of IBHV(SK) with establishment of a disease model in chickens will facilitate the development of type-specific diagnostic reagents and assays for the evaluation of potential experimental vaccines against pathogenic FAdV infections.
authors.affiliation: Vaccine and Infectious Disease Organization, 120 Veterinary Road, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3. Arshud.Dar@usask.ca
authors.forename: Arshud
authors.initials: A
authors.lastname: Dar
authors.forename: Susantha
authors.initials: S
authors.lastname: Gomis
authors.forename: Ian
authors.initials: I
authors.lastname: Shirley
authors.forename: George
authors.initials: G
authors.lastname: Mutwiri
authors.forename: Robert
authors.initials: R
authors.lastname: Brownlie
authors.forename: Andrew
authors.initials: A
authors.lastname: Potter
authors.forename: Volker
authors.initials: V
authors.lastname: Gerdts
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Tikoo
mesh_terms: D000257:Adenoviridae Infections / Q000453:epidemiology / Q000401:mortality / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D000259:Aviadenovirus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002645:Chickens; D006524:Hepatitis, Viral, Animal / Q000453:epidemiology / Q000401:mortality / Q000473:pathology*; D007181:Inclusion Bodies, Viral / Q000473:pathology / Q000821:virology; D008099:Liver / Q000166:cytology / Q000473:pathology* / Q000821:virology; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000401:mortality / Q000473:pathology*; D012525:Saskatchewan / Q000453:epidemiology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
chemical_list: D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus