title: RUNX3 interactome reveals novel centrosomal targeting of RUNX family of transcription factors.
abstract: RUNX family proteins are critical regulators of lineage differentiation during development. The high prevalence of RUNX mutation/epigenetic inactivation in human cancer indicates a causative role for dysfunctional RUNX in carcinogenesis. This is supported by well-documented evidence of functional interaction of RUNX with components of major oncogenic or tumor suppressive signaling pathways such as TGFβ and Wnt. Here, we explore the binding partners of RUNX3 proteins to further define the scope of RUNX3 function. Using a mass spectrometry-based approach, we found that RUNX3 binds to centrosomal protein rootletin. This led us to uncover the presence of RUNX proteins at the centrosome. Our findings suggest a potential function for RUNX3 during mitosis.
authors.affiliation: Cancer Science Institute of Singapore; National University of Singapore; Singapore.
authors.forename: Linda Shyue Huey
authors.initials: LS
authors.lastname: Chuang
authors.forename: Soak Kuan
authors.initials: SK
authors.lastname: Lai
authors.forename: Maki
authors.initials: M
authors.lastname: Murata-Hori
authors.forename: Ayumi
authors.initials: A
authors.lastname: Yamada
authors.forename: Hoi-Yeung
authors.initials: HY
authors.lastname: Li
authors.forename: Jayantha
authors.initials: J
authors.lastname: Gunaratne
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000818:Animals; D019556:COS Cells; D045744:Cell Line, Tumor; D018385:Centrosome / Q000378:metabolism*; D002522:Chlorocebus aethiops; D050659:Core Binding Factor Alpha 1 Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D050676:Core Binding Factor Alpha 2 Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D050677:Core Binding Factor Alpha 3 Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry; D008938:Mitosis; D015739:Nocodazole / Q000494:pharmacology; D011485:Protein Binding; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction; D014404:Tubulin / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: C499237:CROCC protein, human; D050659:Core Binding Factor Alpha 1 Subunit; D050676:Core Binding Factor Alpha 2 Subunit; D050677:Core Binding Factor Alpha 3 Subunit; D003598:Cytoskeletal Proteins; D034741:RNA, Small Interfering; D014404:Tubulin; D051153:Wnt Proteins; D015739:Nocodazole