title: Lipid fingerprinting in women with early-onset preeclampsia: a first look.
abstract: OBJECTIVE
  The aim of this preliminary study was to characterize the plasma lipid profiling of women with preeclampsia.
  
  
  METHODS
  Plasma samples of 8 pregnant women with early-onset preeclampsia and 8 normal pregnant women were evaluated. Lipids were extracted from plasma using the Bligh-Dyer protocol. The extracts were subjected to MALDI-MS. Data matrix was exported for partial least squares discriminant analysis (PLS-DA) and a parameter VIP was employed to reflect the variable importance in the discriminant analysis. The major discriminant variables were selected and underwent to Mann-Whitney U test.
  
  
  RESULTS
  A total of 1290 ions were initially identified and twelve m/z signals were highlighted as the most important lipids for the discrimination of patients with preeclampsia. The identification of these differential lipids was carried out through Lipid Database Search.
  
  
  CONCLUSIONS
  The main classes identified were glycerophosphocholines [GP01], glycerophosphoserines [GP03], glycerophosphoglycerols [GP04], glycosyldiradylglycerols [GL05] and glycerophosphates [GP10].
authors.affiliation: Obstetrics Department, Universidade Federal de São Paulo, Brazil. Leminas@ig.com.br
authors.forename: Leandro
authors.initials: L
authors.lastname: De Oliveira
authors.forename: Niels Olsen S
authors.initials: NO
authors.lastname: Câmara
authors.forename: Tatiana
authors.initials: T
authors.lastname: Bonetti
authors.forename: Edson G
authors.initials: EG
authors.lastname: Lo Turco
authors.forename: Ricardo P
authors.initials: RP
authors.lastname: Bertolla
authors.forename: Antonio F
authors.initials: AF
authors.lastname: Moron
authors.forename: Nelson
authors.initials: N
authors.lastname: Sass
authors.forename: Ismael Dale Cotrim Guerreiro
authors.initials: ID
authors.lastname: Da Silva
mesh_terms: D000328:Adult; D016002:Discriminant Analysis; D005260:Female; D005994:Glycerophosphates / Q000097:blood; D005997:Glycerylphosphorylcholine / Q000097:blood; D006801:Humans; D016018:Least-Squares Analysis; D008055:Lipids / Q000097:blood* / Q000737:chemistry; D010768:Phosphoserine / Q000031:analogs & derivatives / Q000097:blood; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D055815:Young Adult
chemical_list: D005994:Glycerophosphates; D008055:Lipids; C409185:diglycerol phosphate; D010768:Phosphoserine; C067651:glycerophosphoserine; D005997:Glycerylphosphorylcholine