title: Modulation of gene expression in cold-induced sweetening resistant potato species Solanum berthaultii exposed to low temperature.
abstract: Cold-induced sweetening (CIS) is a crucial factor influencing the processing quality of potato tubers. To better understand the molecular events of potato CIS and different CIS-sensitivity among various potato species, a suppression subtractive hybridization library and cDNA microarray gene filters were developed. A total of 188 genes were found to be differentially expressed (DE) in Solanum berthaultii (ber) upon cold stimulation. These functional genes were mostly related to cell rescue, defense and virulence, metabolism, energy and protein fate, included in various processes of plant defense against abiotic stresses. Four expression patterns of these DE genes were profiled by qRT-PCR using the cold-stored tubers of both CIS-resistant (ber) and CIS-sensitive (E-potato 3, a variety of S. tuberosum) potatoes. The expression pattern and abundance of many DE genes encoding proteins involved in metabolism were different in these two potato tubers, especially genes associated with amylolysis, sucrose decomposition and glycolysis pathways, indicating distinct regulatory mechanisms between ber and E3 in response to cold stress, which may be crucial for potato CIS. Further investigation of these cold-regulated genes will deepen our understanding of the regulatory mechanisms of potato CIS and direct approaches for the genetic improvement of potato processing quality.
authors.affiliation: National Centre for Vegetable Improvement (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Botao
authors.initials: B
authors.lastname: Song
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Jianwen
authors.initials: J
authors.lastname: Yang
authors.forename: Tianjiu
authors.initials: T
authors.lastname: He
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Huiling
authors.initials: H
authors.lastname: Zhang
authors.forename: Conghua
authors.initials: C
authors.lastname: Xie
mesh_terms: D050260:Carbohydrate Metabolism / Q000235:genetics; D003080:Cold Temperature; D018744:DNA, Plant / Q000235:genetics; D005511:Food Handling; D061353:Food Storage; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006019:Glycolysis / Q000235:genetics; D015416:Maillard Reaction; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D032322:Solanum / Q000235:genetics* / Q000502:physiology; D013395:Sucrose / Q000378:metabolism
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D013395:Sucrose