title: PEGylated protein separations: challenges and opportunities.
abstract: PEGylation, the covalent attachment of polyethylene glycol (PEG) chains to protein, isa promising method for making an efficient protein drug. Several PEGylated protein drugs, such as PEGylated interferons, are already on the market and others are presently in their clinical trials. However, the PEGylation reaction is very product specific so that generalized or platform processes for both reaction and purification have not yet been established. In the current issue of Biotechnology Journal, Günter Allmaier and colleagues report a modified microchip capillary gel electrophoresis (MCGE), which allows for a rapid separation (one minute) of PEGylated proteins of different degrees of PEGylation.
authors.affiliation: Bioprocess Engineering Laboratory, School of Engineering and Graduate School of Medicine, Yamaguchi University, Ube, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Yoshimoto
authors.forename: Shuichi
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D046209:Electrophoresis, Microchip / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D011092:Polyethylene Glycols