title: Multiple in vitro bioassay approach in sediment toxicity evaluation: Masan Bay, Korea.
abstract: Extracts of 21 sediment samples from Masan Bay, Korea, used in an earlier chemical measurement, were screened for their ability to induce estrogen, - and dioxin - like gene expression using the E-Assay (+), DR-CALUX assay, respectively, and to inhibit acetylcholinesterase (AChE) activity using an in vitro AChE assay. Biological impact in the industry-rich inner bay is higher than outer bay. DDTs (0.65), coplanar PCBs (0.77), HCHs (0.64), PAHs (0.61) and APs (0.53) with good correlation to E-assay (+) are seen as environmental estrogens. The highest induction of DR-CALUX response was seen again at station M12 and 15 which received sewage effluents. PCDD/DFs gave the highest correlation (0.75). Interestingly, the M12 station at the sewage treatment outlet showed the highest activity. Among the targeted chemicals APs (0.66), PCBs (0.64), PAHs (0.61) and DDT (0.49) correlated well with the AChE bioassay. Spearman rank correlation on analytical and biochemical results affirmed the 'hot spots' and point sources (e.g., sewage treatment and industrial outfall) and suspected toxicants. Significant correlations between organo chlorine pesticides, PCBs, dioxins and alkylphenols and their biological effects were observed.
authors.affiliation: Oil and POPs Research Group, Korean Ocean Research and Development Institute, 391 Jangmok Myon, Geoje, Korea.
authors.forename: Jee-Hyun
authors.initials: JH
authors.lastname: Jung
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Hong
authors.forename: Un Hyuk
authors.initials: UH
authors.lastname: Yim
authors.forename: Sung Yong
authors.initials: SY
authors.lastname: Ha
authors.forename: Won Joon
authors.initials: WJ
authors.lastname: Shim
authors.forename: N
authors.initials: N
authors.lastname: Kannan
mesh_terms: D061006:Bays / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000633:toxicity; D004147:Dioxins / Q000032:analysis / Q000633:toxicity; D004784:Environmental Monitoring / Q000379:methods*; D015870:Gene Expression / Q000187:drug effects; D019015:Geologic Sediments / Q000737:chemistry*; D010575:Pesticides / Q000032:analysis / Q000633:toxicity; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity; D056910:Republic of Korea; D018570:Risk Assessment; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D002800:Cholinesterase Inhibitors; D004147:Dioxins; D010575:Pesticides; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls