title: Influence of growth phase on harvesting of Chlorella zofingiensis by dissolved air flotation.
abstract: The effects of changes in cellular characteristics and dissolved organic matter (DOM) on dissolved air flotation (DAF) harvesting of Chlorella zofingiensis at the different growth phases were studied. Harvesting efficiency increased with Al(3+) dosage and reached more than 90%, regardless of growth phases. In the absence of DOM, the ratio of Al(3+) dosage to surface functional group concentration determined the harvesting efficiency. DOM in the culture medium competed with algal cell surface functional groups for Al(3+), and more Al(3+) was required for cultures with DOM than for DOM-free cultures to achieve the same harvesting efficiency. As the culture aged, the increase of Al(3+) dosage due to increased DOM was less than the decrease of Al(3+) dosage associated with reduced cell surface functional groups, resulting in overall reduced demand for Al(3+). The interdependency of Al(3+) dosage and harvesting efficiency on concentrations of cell surface functional groups and DOM was successfully modeled.
authors.affiliation: Department of Applied Sciences and Mathematics, Arizona State University, Polytechnic Campus, Mesa, AZ 85212, United States. xuezhi.zhang@asu.edu
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Zhang
authors.forename: Pasquale
authors.initials: P
authors.lastname: Amendola
authors.forename: John C
authors.initials: JC
authors.lastname: Hewson
authors.forename: Milton
authors.initials: M
authors.lastname: Sommerfeld
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000388:Air; D000535:Aluminum / Q000494:pharmacology; D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D002708:Chlorella / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D016018:Least-Squares Analysis; D009930:Organic Chemicals / Q000378:metabolism; D012995:Solubility / Q000187:drug effects
chemical_list: D003470:Culture Media; D009930:Organic Chemicals; D000535:Aluminum