title: Bmcc1s, a novel brain-isoform of Bmcc1, affects cell morphology by regulating MAP6/STOP functions.
abstract: The BCH (BNIP2 and Cdc42GAP Homology) domain-containing protein Bmcc1/Prune2 is highly enriched in the brain and is involved in the regulation of cytoskeleton dynamics and cell survival. However, the molecular mechanisms accounting for these functions are poorly defined. Here, we have identified Bmcc1s, a novel isoform of Bmcc1 predominantly expressed in the mouse brain. In primary cultures of astrocytes and neurons, Bmcc1s localized on intermediate filaments and microtubules and interacted directly with MAP6/STOP, a microtubule-binding protein responsible for microtubule cold stability. Bmcc1s overexpression inhibited MAP6-induced microtubule cold stability by displacing MAP6 away from microtubules. It also resulted in the formation of membrane protrusions for which MAP6 was a necessary cofactor of Bmcc1s. This study identifies Bmcc1s as a new MAP6 interacting protein able to modulate MAP6-induced microtubule cold stability. Moreover, it illustrates a novel mechanism by which Bmcc1 regulates cell morphology.
authors.affiliation: Collège de France, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7241/Institut National de la Santé et de la Recherche Médicale U1050, Paris, France.
authors.forename: Jessica
authors.initials: J
authors.lastname: Arama
authors.forename: Anne-Cécile
authors.initials: AC
authors.lastname: Boulay
authors.forename: Christophe
authors.initials: C
authors.lastname: Bosc
authors.forename: Christian
authors.initials: C
authors.lastname: Delphin
authors.forename: Damarys
authors.initials: D
authors.lastname: Loew
authors.forename: Philippe
authors.initials: P
authors.lastname: Rostaing
authors.forename: Edwige
authors.initials: E
authors.lastname: Amigou
authors.forename: Pascal
authors.initials: P
authors.lastname: Ezan
authors.forename: Laure
authors.initials: L
authors.lastname: Wingertsmann
authors.forename: Laurent
authors.initials: L
authors.lastname: Guillaud
authors.forename: Annie
authors.initials: A
authors.lastname: Andrieux
authors.forename: Christian
authors.initials: C
authors.lastname: Giaume
authors.forename: Martine
authors.initials: M
authors.lastname: Cohen-Salmon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D003080:Cold Temperature; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000187:drug effects / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects* / Q000502:physiology; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; C491893:Mtap6 protein, mouse; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; C560677:PRUNE2 protein, mouse; D020033:Protein Isoforms