title: Comparison of fast gas chomatography-surface acoustic wave (FGC-SAW) detection and GC-MS for characterizing blueberry cultivars and maturity.
abstract: A novel analytical method using fast gas chromatography-surface acoustic wave detection (FGC-SAW) was employed to rapidly characterize blueberry volatile profiles according to genotypes and fruit maturity. Fourteen FGC-SAW peaks were observed and 11 peaks were tentatively identified in the 15 s chromatogram. Peak identifications were confirmed by matching retention index values with similar values from GC-MS analyses of the same samples. Eighty peaks were observed in the 40 min GC-MS analysis of identical samples. Principal component analysis (PCA) score plots of FGC-SAW and GC-MS data both differentiated blueberries according to genotype, maturity stage, and harvest date even though FGC-SAW PCA's used far fewer peak area values. PCA plots clearly separated 'FL02-40', 'Snowchaser', 'Jewel', and 'Primadonna' blueberry cultivars into four quarters using two-dimensional PCA projections. FGC-SAW was also successful in differentiating three berry maturity stages in PCA score plots for both 'Jewel' and 'Primadonna' cultivars. FGC-SAW is an effective technique for rapid analysis of major blueberry volatiles, but could not determine many mid- and low-level volatiles as they were often coeluted with higher concentration volatiles.
authors.affiliation: Citrus Research and Education Center, University of Florida , 700 Experiment Station Road, Lake Alfred, Florida 33850, USA.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Du
authors.forename: James
authors.initials: J
authors.lastname: Olmstead
authors.forename: Russell
authors.initials: R
authors.lastname: Rouseff
mesh_terms: D029798:Blueberry Plants / Q000737:chemistry* / Q000235:genetics; D002849:Chromatography, Gas / Q000379:methods*; D005638:Fruit / Q000737:chemistry* / Q000145:classification* / Q000254:growth & development; D008401:Gas Chromatography-Mass Spectrometry; D005838:Genotype; D013016:Sound; D013045:Species Specificity; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D055549:Volatile Organic Compounds