title: Evaluation of osteoinduction and proliferation on nano-Sr-HAP: a novel orthopedic biomaterial for bone tissue regeneration.
abstract: Hydroxyapatite (HAP), a CaP compound similar to the mineral phase present in bone, has excellent biocompatibility but little osseous inductivity. In this study, we evaluated the novel nano-Sr-HAP, in which the calcium of hydroxyapatite was substituted with strontium, which acts as a bone-forming agent. Its biocompatibility and osteoinduction were assayed using marrow mesenchymal stem cells (MSCs) and osteoblasts (OBs) in vitro. We were able to demonstrate that nano-Sr-HAP supported increased OB cell adhesion, proliferation and viability up to 4 days in culture when compared with nano-HAP. MSCs cultured with nano-Sr-HAP showed higher alkaline phosphatase (ALP) activity. More extracellular mineralized nodules were found with nano-Sr-HAP compared to nano-HAP, especially in images of ALP staining. We suggest that nano-Sr-HAP powders possess osteoconductive and osteoinductive properties and have the potential to be used in the repair of bone defects caused by osteoporotic fractures.
authors.affiliation: Department of Orthopaedics, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200011, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Hao
authors.forename: Huanqing
authors.initials: H
authors.lastname: Yan
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Wang
authors.forename: Bangshang
authors.initials: B
authors.lastname: Zhu
authors.forename: Congqin
authors.initials: C
authors.lastname: Ning
authors.forename: Shengfang
authors.initials: S
authors.lastname: Ge
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000138:chemical synthesis* / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects*; D013324:Strontium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D018786:Bone Substitutes; D017886:Durapatite; D013324:Strontium