title: Novel erythropoietin-loaded nanoparticles with prolonged in vivo response.
abstract: The aim of this study was to incorporate human recombinant erythropoietin (EPO) in biodegradable polymeric nanoparticles targeting a prolonged-release effect. EPO-loaded poly(DL-lactide-co-glycolide) nanoparticles were prepared using double emulsion method (w/o/w) with least process-related stress on the encapsulated drug. The nanoparticles have been fully characterized including in vitro release profile. The biological activity was assessed in vivo using BALB-c mice. The produced particles appeared spherical in shape with smooth regular surfaces and had an average particle size of 225.9 ± 3.8 nm. The entrapment efficiency was 33.3%. The in vitro release profile exhibited a biphasic mode with a burst of 50% cumulative drug release, followed by a slow rate of release over 24 h, reaching a maximum of 82%. The bioassay results showed that EPO-loaded nanoparticles were able to maintain the physiological activity of EPO for 14 days after single subcutaneous injection compared with pure and marketed EPO formulae (EPREX®).
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Bahgat E
authors.initials: BE
authors.lastname: Fayed
authors.forename: Abdulkader F
authors.initials: AF
authors.lastname: Tawfik
authors.forename: Alaa Eldeen B
authors.initials: AE
authors.lastname: Yassin
mesh_terms: D000818:Animals; D003692:Delayed-Action Preparations / Q000493:pharmacokinetics / Q000494:pharmacology; D004655:Emulsions / Q000493:pharmacokinetics / Q000494:pharmacology; D004921:Erythropoietin / Q000493:pharmacokinetics* / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles; D010316:Particle Size; D011994:Recombinant Proteins / Q000493:pharmacokinetics / Q000494:pharmacology; D013997:Time Factors
chemical_list: D003692:Delayed-Action Preparations; C554270:EPO protein, human; D004655:Emulsions; D011994:Recombinant Proteins; D004921:Erythropoietin