title: Development of real-time radioisotope imaging systems for plant nutrient uptake studies.
abstract: Ionic nutrition is essential for plant development. Many techniques have been developed to image and (or) measure ionic movement in plants. Nevertheless, most of them are destructive and limit the analysis. Here, we present the development of radioisotope imaging techniques that overcome such restrictions and allow for real-time imaging of ionic movement. The first system, called macroimaging, was developed to visualize and measure ion uptake and translocation between organs at a whole-plant scale. Such a device is fully compatible with illumination of the sample. We also modified fluorescent microscopes to set up various solutions for ion uptake analysis at the microscopic level. Both systems allow numerical analysis of images and possess a wide dynamic range of detection because they are based on radioactivity.
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Kanno
authors.forename: Masato
authors.initials: M
authors.lastname: Yamawaki
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ishibashi
authors.forename: Natsuko I
authors.initials: NI
authors.lastname: Kobayashi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hirose
authors.forename: Keitaro
authors.initials: K
authors.lastname: Tanoi
authors.forename: Laurent
authors.initials: L
authors.lastname: Nussaume
authors.forename: Tomoko M
authors.initials: TM
authors.lastname: Nakanishi
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D001692:Biological Transport; D003470:Culture Media / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D058085:Iron Compounds / Q000378:metabolism; D007553:Isotope Labeling / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D012275:Oryza / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010759:Phosphorus Isotopes / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D003470:Culture Media; D058085:Iron Compounds; D010710:Phosphates; D010759:Phosphorus Isotopes; D014867:Water