title: Detection of virulence-associated genes in Staphylococcus aureus isolated from bovine clinical mastitis milk samples in Guangxi.
abstract: Staphylococcus aureus is recognized worldwide as a pathogen causing many serious diseases in humans and animals and is one of the most common etiological agents of clinical and subclinical bovine mastitis. The purpose of this study was to determine the presence of genes encoding clfA, fnbA, fnbB, cap5, cap8, hla, hlb, nuc, sea, and tst of S. aureus strains (n = 39) isolated from bovine clinical mastitis in Guangxi by polymerase chain reaction amplification. The results of the present study indicated that all isolates were found to contain one or more virulence-associated genes. The most frequently encountered genes were fnbA (97 %) and nuc (90 %), followed by hla (85 %) and hlb (82 %), respectively. None of the investigated S. aureus strains harbored fnbB and sea genes. The data in the present study showed a relatively wide distribution of the genes fnbA and nuc among the investigated isolates, indicating that they play an important role on bovine mastitis pathogenesis. The study provides a valuable insight into the virulence-associated genes of this important pathogen.
authors.affiliation: Guangxi Key Laboratory of Buffalo Genetics and Breeding, Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, Peoples Republic of China.
authors.forename: Feng Li
authors.initials: FL
authors.lastname: Yang
authors.forename: Xiao Shan
authors.initials: XS
authors.lastname: Li
authors.forename: Xian Wei
authors.initials: XW
authors.lastname: Liang
authors.forename: Xiu Fang
authors.initials: XF
authors.lastname: Zhang
authors.forename: Guang Sheng
authors.initials: GS
authors.lastname: Qin
authors.forename: Bing Zhuang
authors.initials: BZ
authors.lastname: Yang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D002417:Cattle; D002681:China / Q000453:epidemiology; D005260:Female; D008414:Mastitis, Bovine / Q000453:epidemiology* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins