title: Impaired death-associated protein kinase-mediated survival signals in             5-fluorouracil-resistant human endometrial adenocarcinoma cells.
abstract: A recent study showed that both 5-fluorouracil (5FU)-stimulated apoptosis             and Fas-mediated apoptosis in human endometrial adenocarcinoma cells are enhanced             by targeted knockdown of endogenous death-associated protein kinase (DAPK) with             DAPK small-interfering RNAs. Therefore, we investigated the DAPK survival signals             in three 5FU-resistant subclones. DAPK knockdown did not enhance 5FU-stimulated             or Fas-mediated apoptosis in any of the three 5FU-resistant subclones, but the             subclones acquired resistance to VP16-stimulated cell death that was DAPK-independent.             Semi-quantitative flow cytometric analyses showed that there was no differential             expression in nine cell surface antigens, including Fas, and six intracellular             molecules, including DAPK, that may regulate cell death or survival between the             parent cells and 5FU-resistant cells. DAPK mRNA and protein were expressed in             the 5FU-resistant subclones at similar levels to the parent cells. These results             indicate that acquisition of 5FU-resistance may be accompanied by impairment of             common apoptotic signals regulating both DAPK-dependent and DAPK-independent pathways.
authors.affiliation: Santamaria Hospital, 13-15 Shinjo-cho, Ibaraki, Osaka 567-0884, Japan. tetanaka@santamaria-med.jp
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Tanaka
authors.forename: Tao
authors.initials: T
authors.lastname: Bai
authors.forename: Saori
authors.initials: S
authors.lastname: Toujima
authors.forename: Tomoko
authors.initials: T
authors.lastname: Utsunomiya
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Utsunomiya
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yukawa
authors.forename: Junko
authors.initials: J
authors.lastname: Tanaka
mesh_terms: D000230:Adenocarcinoma; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D064550:Death-Associated Protein Kinases; D019008:Drug Resistance, Neoplasm; D016889:Endometrial Neoplasms; D005260:Female; D005434:Flow Cytometry; D005472:Fluorouracil / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D034622:RNA Interference; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: D000964:Antimetabolites, Antineoplastic; D051017:Apoptosis Regulatory Proteins; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D005472:Fluorouracil