title: Rab5 proteins regulate activation and localization of target of rapamycin complex 1.
abstract: The mechanistic target of rapamycin (mTOR) complex 1 is regulated by small GTPase activators and localization signals. We examine here the role of the small GTPase Rab5 in the localization and activation of TORC1 in yeast and mammalian cells. Rab5 mutants disrupt mTORC1 activation and localization in mammalian cells, whereas disruption of the Rab5 homolog in yeast, Vps21, leads to decreased TORC1 function. Additionally, regulation of PI(3)P synthesis by Rab5 and Vps21 is essential for TORC1 function in both contexts.
authors.affiliation: Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors.forename: Dave
authors.initials: D
authors.lastname: Bridges
authors.forename: Kaleigh
authors.initials: K
authors.lastname: Fisher
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Zolov
authors.forename: Tingting
authors.initials: T
authors.lastname: Xiong
authors.forename: Ken
authors.initials: K
authors.lastname: Inoki
authors.forename: Lois S
authors.initials: LS
authors.lastname: Weisman
authors.forename: Alan R
authors.initials: AR
authors.lastname: Saltiel
mesh_terms: D002460:Cell Line; D006801:Humans; D000076222:Mechanistic Target of Rapamycin Complex 1; D046912:Multiprotein Complexes; D018129:Phosphatidylinositol Phosphates / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D058570:TOR Serine-Threonine Kinases; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D020696:rab5 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D046912:Multiprotein Complexes; D018129:Phosphatidylinositol Phosphates; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; D000076222:Mechanistic Target of Rapamycin Complex 1; D058570:TOR Serine-Threonine Kinases; C086866:VPS21 protein, S cerevisiae; D020691:rab GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins