title: Effect of a special carbohydrate-protein cake on oxidative stress markers after exhaustive cycling in humans.
abstract: Exercise has been associated with oxidative stress that is correlated with muscle fatigue and reduced exercise performance. The aim of this study was to examine the effects of a special cake (consisting of carbohydrate to whey protein 3.5:1) vs an isocaloric carbohydrate cake on biomarkers of oxidative stress in 9 males after exhaustive cycling. A randomized single-blind cross-over study was completed. They performed one trial involving a 2-h exercise on a cycle ergometer at 60-65% VO(2)max followed by a 4-h recovery and then a second trial involved an 1-h exercise at 60-65% VO(2)max which was increased at 95% VO(2)max (time trial). The subjects received 4 experimental or placebo cakes after the first trial (the first immediately after and then one every hour). Blood samples were collected at eight time intervals: pre-exercise, 30 min, 1.5 h and 4 h post-exercise, post time Trial, 1 h, 24 h and 48 h post time Trial. Thiobarbituric Acid Reactive Substances (TBARS), protein carbonyls, total antioxidant capacity (TAC), catalase and glutathione (GSH) were determined spectrophotometrically. The mean time to exhaustion did not differ upon cake consumption. Consumption of the special cake reduced TBARS significantly, but had no effect on other oxidative stress markers.
authors.affiliation: Department of Biochemistry-Biotechnology, University of Thessaly, Larisa 41221, Greece. e-f-thalia@hotmail.com
authors.forename: Efthalia
authors.initials: E
authors.lastname: Kerasioti
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kiskini
authors.forename: Aristidis
authors.initials: A
authors.lastname: Veskoukis
authors.forename: Athanasios
authors.initials: A
authors.lastname: Jamurtas
authors.forename: Christina
authors.initials: C
authors.lastname: Tsitsimpikou
authors.forename: Aristidis M
authors.initials: AM
authors.lastname: Tsatsakis
authors.forename: Yiannis
authors.initials: Y
authors.lastname: Koutedakis
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stagos
authors.forename: Demetrios
authors.initials: D
authors.lastname: Kouretas
authors.forename: Vaios
authors.initials: V
authors.lastname: Karathanos
mesh_terms: D000328:Adult; D001642:Bicycling; D015415:Biomarkers; D018592:Cross-Over Studies; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004044:Dietary Proteins / Q000008:administration & dosage*; D006801:Humans; D018384:Oxidative Stress; D005082:Physical Exertion; D016037:Single-Blind Method
chemical_list: D015415:Biomarkers; D004040:Dietary Carbohydrates; D004044:Dietary Proteins