title: Bergmann glia modulate cerebellar Purkinje cell bistability via Ca2+-dependent K+ uptake.
abstract: Recent studies have shown that cerebellar Bergmann glia display coordinated Ca(2+) transients in live mice. However, the functional significance of Bergmann glial Ca(2+) signaling remains poorly understood. Using transgenic mice that allow selective stimulation of glial cells, we report here that cytosolic Ca(2+) regulates uptake of K(+) by Bergmann glia, thus providing a powerful mechanism for control of Purkinje cell-membrane potential. The decline in extracellular K(+) evoked by agonist-induced Ca(2+) in Bergmann glia transiently increased spike activity of Purkinje cells in cerebellar slices as well as in live anesthetized mice. Thus, Bergmann glia play a previously unappreciated role in controlling the membrane potential and thereby the activity of adjacent Purkinje cells.
authors.affiliation: Division of Glial Disease and Therapeutics, Center for Translational Neuromedicine, University of Rochester, Rochester, NY 14642, USA. fushun_wang@urmc.rochester.edu
authors.forename: Fushun
authors.initials: F
authors.lastname: Wang
authors.forename: Qiwu
authors.initials: Q
authors.lastname: Xu
authors.forename: Weishan
authors.initials: W
authors.lastname: Wang
authors.forename: Takahiro
authors.initials: T
authors.lastname: Takano
authors.forename: Maiken
authors.initials: M
authors.lastname: Nedergaard
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D020013:Calcium Signaling / Q000502:physiology*; D002531:Cerebellum / Q000166:cytology*; D008564:Membrane Potentials / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008839:Microelectrodes; D018613:Microscopy, Confocal; D009457:Neuroglia / Q000502:physiology*; D011188:Potassium / Q000378:metabolism*; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011188:Potassium