title: Evaluation of the efficacy of a recombinant Entamoeba histolytica cysteine proteinase (EhCP112) antigen in minipig.
abstract: Cysteine proteinases 112 (EhCP112) of Entamoeba histolytica are considered important for ameba pathogenicity. The recombinant gene was obtained by cloning and expression of the EhCP112 gene in heterologous host Escherichia coli BL-21 (DE3), were used to evaluate their ability to induce immune protective responses in minipig against challenge infection in a minipig-E. histolytica model. There was a 46.29% reduction (P<0.001) in the group of recovery of challenged E. histolytica compared with that in the control group. Specific anti-EhCP112 antibodies from immune protected minipig had significantly higher levels of immunoglobulin G (IgG) (P<0.001). This is a first report demonstrating that a recombinant form of EhCP112 generated in E. coli, to immunize a minipig model of E. histolytica, and there is significant protection. This study may help to understand the EhCP112 for human in the future.
authors.affiliation: Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China. heguangzhi7711@163.com
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: He
authors.forename: Shu-Xuan
authors.initials: SX
authors.lastname: Deng
authors.forename: Chuan-Wei
authors.initials: CW
authors.lastname: An
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology; D015153:Blotting, Western; D057056:Cysteine Proteases / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D004749:Entamoebiasis / Q000517:prevention & control*; D005260:Female; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D016052:Protozoan Vaccines / Q000592:standards*; D011817:Rabbits; D013552:Swine; D013556:Swine, Miniature; D014614:Vaccines, Synthetic / Q000592:standards
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007106:Immune Sera; D007074:Immunoglobulin G; D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic; D057056:Cysteine Proteases