title: Translation elongation factor-1A1 (eEF1A1) localizes to the spine by domain III.
abstract: In vertebrates, there are two variants of eukaryotic peptide elongation factor 1A (eEF1A; formerly eEF-1α), eEF1A1 and eEF1A2, which have three well-conserved domains (D(I), D(II), and D(III)). In neurons, eEF1A1 is the embryonic type, which is expressed during embryonic development as well as the first two postnatal weeks. In the present study, EGFP-tagged eEF1A1 truncates were expressed in cortical neurons isolated from rat embryo (E18-19). Live cell images of transfected neurons showed that D(III)-containing EGFP-fusion proteins (EGFP-D(III), -D(II)-III, -D(I)-III) formed clusters that were confined within somatodendritic domains, while D(III)-missing ones (EGFP-D(I), -D(II), -D(I)-II) and control EGFP were homogeneously D(I)spersed throughout the neuron incluD(I)ng axons. In dendrites, EGFP-D(III) was targeted to the heads of spine- and filopoD(I)a-like protrusions, where it was colocalized with SynGAPα, a postsynaptic marker. Our data inD(I)cate that D(III) of eEF1A1 meD(I)ates formation of clusters and localization to spines.
authors.affiliation: Department of Anatomy, Dongguk University College of Medicine, Gyeongju, Korea.
authors.forename: Sun-Jung
authors.initials: SJ
authors.lastname: Cho
authors.forename: Hyunsook
authors.initials: H
authors.lastname: Lee
authors.forename: Samikshan
authors.initials: S
authors.lastname: Dutta
authors.forename: Dae-Hyun
authors.initials: DH
authors.lastname: Seog
authors.forename: Il Soo
authors.initials: IS
authors.lastname: Moon
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005333:Fetus / Q000166:cytology / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011554:Pseudopodia / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013131:Spine / Q000166:cytology / Q000378:metabolism*
chemical_list: D020648:Peptide Elongation Factor 1; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins