title: HSV-1 miR-H6 inhibits HSV-1 replication and IL-6 expression in human corneal epithelial cells in vitro.
abstract: HSV-1 infection in the cornea could lead to blindness. The infected cell polypeptide 4 (ICP4) of herpes simplex virus 1 (HSV-1) is a regulator of viral transcription that is required for productive infection. It has been previously demonstrated that miR-H6 encoded from HSV-1 genome targets ICP4 to help maintain latency. In this study, synthesized miR-H6 mimics were transfected into HSV-1-infected human cornea epithelial (HCE) cells. The inhibition of HSV-1 replication and viral ICP4 expression in miR-H6-transfected HCE was confirmed by plaque assay, immunofluorescence, and Western blot. Compared to nontransfection or mock, miR-H6 produced a low-titer HSV-1 and weak ICP4 expression. In addition, miR-H6 can decrease the interleukin 6 released into the medium, which was determined by ELISA. Taken together, the data suggests that miR-H6 targeting of ICP4 inhibits HSV-1 productive infection and decreases interleukin 6 production in HCE, and this may provide an approach to prevent HSV-1 lytic infection and inhibit corneal inflammation.
authors.affiliation: Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-sen University, 54 Xianlie Road, Guangzhou 510060, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Duan
authors.forename: Jingyu
authors.initials: J
authors.lastname: Liao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huang
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Nie
authors.forename: Kaili
authors.initials: K
authors.lastname: Wu
mesh_terms: D002478:Cells, Cultured; D003315:Cornea / Q000276:immunology / Q000473:pathology / Q000821:virology; D004847:Epithelial Cells / Q000276:immunology / Q000473:pathology / Q000821:virology; D015967:Gene Expression Regulation, Viral / Q000276:immunology; D006561:Herpes Simplex / Q000276:immunology / Q000473:pathology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000276:immunology*; D015850:Interleukin-6 / Q000096:biosynthesis; D035683:MicroRNAs / Q000235:genetics / Q000276:immunology*; D018716:Molecular Mimicry; D009843:Oligoribonucleotides / Q000276:immunology; D012367:RNA, Viral / Q000096:biosynthesis / Q000276:immunology*; D014158:Transcription, Genetic / Q000276:immunology; D014162:Transfection; D017735:Virus Latency; D014779:Virus Replication
chemical_list: D017874:Immediate-Early Proteins; D015850:Interleukin-6; D035683:MicroRNAs; D009843:Oligoribonucleotides; D012367:RNA, Viral; C048925:herpes simplex virus, type 1 protein ICP4