title: Profiling the erythrocyte membrane proteome isolated from patients diagnosed with chronic obstructive pulmonary disease.
abstract: Structural and metabolic alterations in erythrocytes play an important role in the pathophysiology of Chronic Obstructive Pulmonary Disease (COPD). Whether these dysfunctions are related to the modulation of erythrocyte membrane proteins in patients diagnosed with COPD remains to be determined. Herein, a comparative proteomic profiling of the erythrocyte membrane fraction isolated from peripheral blood of smokers diagnosed with COPD and smokers with no COPD was performed using differential (16)O/(18)O stable isotope labeling. A total of 219 proteins were quantified as being significantly differentially expressed within the erythrocyte membrane proteomes of smokers with COPD and healthy smokers. Functional pathway analysis showed that the most enriched biofunctions were related to cell-to-cell signaling and interaction, hematological system development, immune response, oxidative stress and cytoskeleton. Chorein (VPS13A), a cytoskeleton related protein whose defects had been associated with the presence of cell membrane deformation of circulating erythrocytes was found to be down-regulated in the membrane fraction of erythrocytes obtained from COPD patients. Methemoglobin reductase (CYB5R3) was also found to be underexpressed in these cells, suggesting that COPD patients may be at higher risk for developing methemoglobinemia. This article is part of a Special Issue entitled: Integrated omics.
authors.affiliation: Laboratório de Proteómica, Departamento de Genética, Instituto Nacional de Saúde Dr. Ricardo Jorge (INSA-IP), Lisboa, Portugal.
authors.forename: Bruno M
authors.initials: BM
authors.lastname: Alexandre
authors.forename: Nuno
authors.initials: N
authors.lastname: Charro
authors.forename: Josip
authors.initials: J
authors.lastname: Blonder
authors.forename: Carlos
authors.initials: C
authors.lastname: Lopes
authors.forename: Pilar
authors.initials: P
authors.lastname: Azevedo
authors.forename: António
authors.initials: A
authors.lastname: Bugalho de Almeida
authors.forename: King C
authors.initials: KC
authors.lastname: Chan
authors.forename: DaRue A
authors.initials: DA
authors.lastname: Prieto
authors.forename: Haleem
authors.initials: H
authors.lastname: Issaq
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Veenstra
authors.forename: Deborah
authors.initials: D
authors.lastname: Penque
mesh_terms: D000328:Adult; D000368:Aged; D002450:Cell Communication; D003599:Cytoskeleton / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D008875:Middle Aged; D018384:Oxidative Stress; D020543:Proteome / Q000096:biosynthesis*; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism*; D015398:Signal Transduction; D012907:Smoking / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D020543:Proteome