title: Serum levels of N-acetyl-aspartate in migraine and tension-type headache.
abstract: Serum levels of N-acetyl-aspartate (NAA) may be considered a useful marker of neuronal functioning. We aimed to measure serum NAA in cohorts of migraine and tension-type headache patients versus controls, performing correlations with main clinical features. A total of 147 migraine patients (including migraine without aura, with aura and chronic migraine), 65 tension-type headache (including chronic and frequent episodic tension-type headache) and 34 sex- and age-matched controls were selected. Serum was stored at -80 °C. Quantification of NAA was achieved by the standard addition approach and analysis was performed with liquid-chromatography-mass-spectrometry (LC/MS) technique. The NAA levels were significantly decreased in migraine group (0.065 ± 0.019 mol/L), compared with both tension-type headache patients (0.078 ± 0.016 mol/L) and controls (0.085 ± 0.013 mol/L). Control subjects were significantly different from migraine with and without aura and chronic migraine, who differed significantly from episodic and chronic tension-type headache. Migraine with aura patients showed lower NAA levels when compared to all the other headache subtypes, including migraine without aura and chronic migraine. In the migraine group, no significant correlation was found between NAA serum levels, and headache frequency, allodynia and interval from the last and the next attack. The low NAA in the serum may be a sign of neuronal dysfunction predisposing to migraine, probably based on reduced mitochondria function.
authors.affiliation: Neuroscience and Sensory System Department, Bari Aldo Moro University, Policlinico General Hospital, Neurological Building, Piazza Giulio Cesare 11, 70124, Bari, Italy. m.detommaso@neurol.uniba.it
authors.forename: Marina
authors.initials: M
authors.lastname: de Tommaso
authors.forename: Edmondo
authors.initials: E
authors.lastname: Ceci
authors.forename: Carmela
authors.initials: C
authors.lastname: Pica
authors.forename: Maria
authors.initials: M
authors.lastname: Trojano
authors.forename: Marianna
authors.initials: M
authors.lastname: Delussi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Franco
authors.forename: Paolo
authors.initials: P
authors.lastname: Livrea
authors.forename: Maddalena
authors.initials: M
authors.lastname: Ruggieri
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000097:blood; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D008881:Migraine Disorders / Q000097:blood*; D018781:Tension-Type Headache / Q000097:blood*; D055815:Young Adult
chemical_list: D001224:Aspartic Acid; C000179:N-acetylaspartate