title: Synthesis of cationic magnetic nanoparticles and evaluation of their gene delivery efficacy in Hep G2 cells.
abstract: Well-defined cationic polymers were synthesized via the reversible addition fragmentation chain transfer polymerization and were subsequently used as stabilizer for the synthesis of cationic iron-oxide magnetic nanoparticles via the coprecipitation method. The surface-coated iron-oxide nanoparticles made stable suspension in water and were characterized by a range of techniques such as dynamic light scattering, transmission electron microscopy, and thermogravimetric analysis. The presence of protonated amine and carbohydrate residues onto the surface of the nanoparticles allowed their facile complexation with DNA and the resulting nanocomplexes were then studied for their efficacy as DNA carriers in Hep G2 cells.
authors.affiliation: Department of Chemical and Materials Engineering, Alberta Ingenuity Centre for Carbohydrate Science, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Jui-Tse
authors.initials: JT
authors.lastname: Chen
authors.forename: Marya
authors.initials: M
authors.lastname: Ahmed
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Liu
authors.forename: Ravin
authors.initials: R
authors.lastname: Narain
mesh_terms: D002412:Cations / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D015870:Gene Expression; D056945:Hep G2 Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D014162:Transfection
chemical_list: D002412:Cations; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D004247:DNA