title: Prevalence of fimA genotypes of Porphyromonas gingivalis and other periodontal bacteria in a Spanish population with chronic periodontitis.
abstract: OBJECTIVE
  The aim of this study was to determine the prevalence of the different fimA genotypes of Porphyromonas gingivalis in adult Spanish patients with chronic periodontitis, patients with gingivitis and periodontally healthy subjects, and the relationship between these genotypes and other periodontopathogenic bacteria.
  
  
  METHODS
  Samples of subgingival plaque were taken from 86 patients (33 with chronic periodontitis, 16 with gingivitis, and 37 periodontally healthy) in the course of a full periodontal examination. PCR was employed to determine the presence of the 6 fimA genotypes of Porphyromonas gingivalis (I-V and Ib) and of Aggregatibacter actinomycetemcomitans, Tannerella forsythia and Treponema denticola.
  
  
  RESULTS
  Porphyromonas gingivalis fimA genotypes II and Ib were present in significantly higher percentages in periodontal patients (39.4% and 12.1% respectively) than in healthy or gingivitis subjects. The prevalence of Tannerella forsythia, Treponema denticola, and Porphyromonas gingivalis fimA genotype IV was significantly higher in the group that presented bleeding greater than 30%. A positive correlation was found between Porphyromonas gingivalis fimA genotype IV and Treponema denticola.
  
  
  CONCLUSIONS
  A strong association between Porphyromonas gingivalis fimA genotypes II and Ib and chronic periodontitis exists in the Spanish population. The most prevalent genotype in periodontal patients is II.
authors.affiliation: Department of Stomatology, University of Valencia, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: Puig-Silla
authors.forename: Francisco
authors.initials: F
authors.lastname: Dasí-Fernández
authors.forename: José-María
authors.initials: JM
authors.lastname: Montiel-Company
authors.forename: José-Manuel
authors.initials: JM
authors.lastname: Almerich-Silla
mesh_terms: D000328:Adult; D055113:Chronic Periodontitis / Q000382:microbiology*; D005260:Female; D036781:Fimbriae Proteins / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000302:isolation & purification; D013030:Spain
chemical_list: C063055:fimbrillin; D036781:Fimbriae Proteins