title: Overexpression of the halophyte Kalidium foliatum H⁺-pyrophosphatase gene confers salt and drought tolerance in Arabidopsis thaliana.
abstract: According to sequences of H(+)-pyrophosphatase genes from GenBank, a new H(+)-pyrophosphatase gene (KfVP1) from the halophyte Kalidium foliatum, a very salt-tolerant shrub that is highly succulent, was obtained by using reverse transcription PCR and rapid amplification of cDNA ends methods. The obtained KfVP1 cDNA contained a 2295 bp ORF and a 242 bp 3'-untranslated region. It encoded 764 amino acids with a calculated molecular mass of 79.78 kDa. The deduced amino acid sequence showed high identity to those of H(+)-PPase of some Chenopodiaceae plant species. Semi-quantitative PCR results revealed that transcription of KfVP1 in K. foliatum was induced by NaCl, ABA and PEG stress. Transgenic lines of A. thaliana with 35S::KfVP1 were generated. Three transgenic lines grew more vigorous than the wild type (ecotype Col-0) under salt and drought stress. Moreover, the transgenic plants accumulated more Na(+) in the leaves compared to wild type plants. These results demonstrated that KfVP1 from K. foliatum may be a functional tonoplast H(+)-pyrophosphatase in contributing to salt and drought tolerance.
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, People's Republic of China. yaomanhong861024@126.com
authors.forename: Manhong
authors.initials: M
authors.lastname: Yao
authors.forename: Youling
authors.initials: Y
authors.lastname: Zeng
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Yunlan
authors.initials: Y
authors.lastname: Huang
authors.forename: Enfeng
authors.initials: E
authors.lastname: Zhao
authors.forename: Fuchun
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics*; D027703:Amaranthaceae / Q000201:enzymology* / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D055864:Droughts; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D043564:Inorganic Pyrophosphatase / Q000235:genetics*; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D030821:Plants, Genetically Modified; D054712:Salinity; D055051:Salt-Tolerant Plants / Q000201:enzymology* / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D043564:Inorganic Pyrophosphatase