title: Simultaneous determination of salidroside and its aglycone metabolite p-tyrosol in rat plasma by liquid chromatography-tandem mass spectrometry.
abstract: Salidroside and its aglycone p-tyrosol are two major phenols in the genus Rhodiola and have been confirmed to possess various pharmacological properties. In our present study, p-tyrosol was identified as the deglycosylation metabolite of salidroside after intravenous (i.v.) administration to rats at a dose of 50 mg/kg, but was not detectable after intragastric gavage (i.g.) administration through HPLC-photodiode array detection (PDA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Next, an accurate and precise LC-MS/MS method was developed to quantitatively determine salidroside and p-tyrosol in rat plasma samples. Samples were analyzed by LC-MS/MS on a reverse-phase xTerra MS C18 column which was equilibrated and eluted with an isocratic mixture of acetonitrile-water (1:9, v/v) at a flow rate of 0.3 mL/min. The analytes were monitored by multiple reaction monitoring (MRM) under the negative electrospray ionization mode. The precursor/product transitions (m/z) were 299.0 → 118.8 for salidroside, 137.0 → 118.9 for p-tyrosol and 150.1 → 106.9 for the internal standard (IS), paracetamol, respectively. The calibration curve was linear over the concentration ranges of 50-2,000 ng/mL for salidroside and 20-200 ng/mL for p-tyrosol. The inter- and intra-day accuracy and precision were within ± 15%. The method has been successfully applied to the pharmacokinetic study and the oral bioavailability was calculated.
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University/Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China. guona0329@126.com
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Hu
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Fan
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.forename: Dehui
authors.initials: D
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Haiying
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D005960:Glucosides / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D008297:Male; D010636:Phenols / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D005960:Glucosides; D010636:Phenols; C011867:4-hydroxyphenylethanol; C009172:rhodioloside; D010626:Phenylethyl Alcohol