title: Biochemical methods for quantifying sphingolipids: ceramide, sphingosine, sphingosine kinase-1 activity, and sphingosine-1-phosphate.
abstract: Sphingolipids (ceramide, sphingosine, and sphingosine-1-phosphate) are bioactive lipids with important biological functions in proliferation, apoptosis, angiogenesis, and inflammation. Herein, we describe easy and rapid biochemical methods with the use of radiolabeled molecules ((3)H, (32)P) for their mass determination. Quantitation of sphingosine kinase-1 activity, the most studied isoform, is also included.
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, CNRS, Toulouse, France.
authors.forename: Leyre
authors.initials: L
authors.lastname: Brizuela
authors.forename: Olivier
authors.initials: O
authors.lastname: Cuvillier
mesh_terms: D002457:Cell Extracts; D045744:Cell Line, Tumor; D002518:Ceramides / Q000032:analysis*; D006801:Humans; D008246:Lysophospholipids / Q000032:analysis*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000032:analysis* / Q000378:metabolism; D013053:Spectrophotometry; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000032:analysis*; D000099157:Sphingosine Kinase
chemical_list: D002457:Cell Extracts; D002518:Ceramides; D008246:Lysophospholipids; D017853:Phosphotransferases (Alcohol Group Acceptor); D013110:Sphingosine; D000099157:Sphingosine Kinase; C060506:sphingosine 1-phosphate