title: Rapid generation of dityrosine cross-linked Aβ oligomers via Cu-redox cycling.
abstract: There is a great interest in the role of free radicals and oxidative stress in Alzheimer's disease and for the role of transition metals in the generation of oligomers of Aβ peptides. In the literature, there are a multitude of varying methods that can be used to create soluble oligomers of Aβ, however, the processes that create these oligomers are often stochastic by nature and thus reproducibility is an issue. Here we report a simple and reproducible method for the production of radically derived dityrosine cross-linked oligomers of Aβ, through reaction with copper and ascorbic acid.
authors.affiliation: Mental Health Research Institute, University of Melbourne, Parkville, VIC, Australia.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Gunn
authors.forename: Blaine R
authors.initials: BR
authors.lastname: Roberts
authors.forename: Ashley I
authors.initials: AI
authors.lastname: Bush
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism; D015151:Immunoblotting; D007700:Kinetics; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D055503:Protein Multimerization / Q000187:drug effects*; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D016229:Amyloid beta-Peptides; D003432:Cross-Linking Reagents; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D017665:Hydroxyl Radical; D014443:Tyrosine; D003300:Copper; C007543:dityrosine; D001205:Ascorbic Acid