title: Identification of genes responsible for biofilm formation or virulence in Salmonella enterica serovar pullorum.
abstract: Salmonella living in biofilms are more resistant to chemical and physical stresses. However, information regarding the regulation of genes involved in biofilm formation for Salmonella enterica serovar Pullorum remains limited. In this study, eight mutants with knockout of genes ompR, rpoS, rfaG, rfbH, rhlE, metE, spiA, or steB from the Salmonella enterica serovar Pullorum strain S6702 were constructed. Phenotypic analysis revealed that all mutants were similar to the wild-type strain in growth rate. Only the ompR mutant showed a complete loss of production ofcurli and biofilm formation. The other mutants showed a modified production of curli and cellulose with less effect related to biofilm formation. The results of animal experiments indicated that the deletion of genes ompR, spiA, rfaG, or metE in wild-type strains contributed to attenuation of virulence in 1-day-old chickens. This study may bring new insights into novel vaccines or therapeutic interventions against Salmonella enterica serovar Pullorum infections.
authors.affiliation: College of Veterinary Medicine, Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Sujuan
authors.initials: S
authors.lastname: Chen
authors.forename: Hongyan
authors.initials: H
authors.lastname: Dong
authors.forename: Hualu
authors.initials: H
authors.lastname: Sun
authors.forename: Daxin
authors.initials: D
authors.lastname: Peng
authors.forename: Xiufan
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D018441:Biofilms; D002645:Chickens; D017353:Gene Deletion; D055786:Gene Knockout Techniques; D008969:Molecular Sequence Data; D010641:Phenotype; D011201:Poultry Diseases / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D019779:Salmonella enterica / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology*; D014774:Virulence