title: Fractal analysis of two-photon microscopic images for diagnosis of nasopharyngeal cancer.
abstract: In this study, two-photon microscopy (TPM) was applied to obtain high-resolution, optically sectioned images with cellular morphology information from normal and cancerous nasopharyngeal tissue. Notable difference of nuclear-cytoplasmic ratio was found between these two groups. In particular, fractal dimension (FD) analysis based on TPM images showed good differentiation between normal and cancerous nasopharyngeal tissues. Our results indicate that TPM coupled with FD analysis methods has the potential to extract diagnostic features for label-free histological diagnosis.
authors.affiliation: Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, People's Republic of China.
authors.forename: Zufang
authors.initials: Z
authors.lastname: Huang
authors.forename: Jianji
authors.initials: J
authors.lastname: Pan
authors.forename: Guannan
authors.initials: G
authors.lastname: Chen
authors.forename: Zuanfang
authors.initials: Z
authors.lastname: Li
authors.forename: Xiwen
authors.initials: X
authors.lastname: Chen
authors.forename: Yongzeng
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
mesh_terms: D001699:Biometry / Q000379:methods; D002467:Cell Nucleus; D048430:Cell Shape; D003593:Cytoplasm; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D010336:Pathology / Q000379:methods*