title: Desulfosporosinus burensis sp. nov., a spore-forming, mesophilic, sulfate-reducing bacterium isolated from a deep clay environment.
abstract: A novel anaerobic, gram-positive, spore-forming, curved rod-shaped, mesophilic and sulfate-reducing bacterium was isolated from pore water collected in a borehole at -490 m in Bure (France). This strain, designated BSREI1(T), grew at temperatures between 5 °C and 30 °C (optimum 25 °C) and at a pH between 6 and 8 (optimum 7). It did not require NaCl for growth, but tolerated it up to 1.5 % NaCl. Sulfate, thiosulfate and elemental sulfur were used as terminal electron acceptors. Strain BSREI1(T) used crotonate, formate, lactate, pyruvate, fructose, glycerol and yeast extract as electron donors in the presence of sulfate. The sole quinone was MK-7. The G+C content of the genomic DNA was 43.3 mol%. Strain BSREI1(T) had the type strains of Desulfosporosinus lacus (16S rRNA gene sequence similarity of 96.83 %), Desulfosporosinus meridiei (96.31 %) and Desulfosporosinus hippei (96.16 %) as its closest phylogenetic relatives. On the basis of phylogenetic and physiological properties, strain BSREI1(T) is proposed as a representative of a novel species of the genus Desulfosporosinus, Desulfosporosinus burensis sp. nov.; the type strain is BSREI1(T) ( = DSM 24089(T) = JCM 17380(T)).
authors.affiliation: ANDRA, Centre de Meuse/Haute-Marne, F-55290 Bure, France.|Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, UMR 235, IRD ESIL MIO, Aix-Marseille Université, case 925, 163 Avenue de Luminy, F-13288 Marseille cedex 9, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Mayeux
authors.affiliation: Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, UMR 235, IRD ESIL MIO, Aix-Marseille Université, case 925, 163 Avenue de Luminy, F-13288 Marseille cedex 9, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
authors.affiliation: Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, UMR 235, IRD ESIL MIO, Aix-Marseille Université, case 925, 163 Avenue de Luminy, F-13288 Marseille cedex 9, France.
authors.forename: Manon
authors.initials: M
authors.lastname: Bartoli-Joseph
authors.affiliation: Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, UMR 235, IRD ESIL MIO, Aix-Marseille Université, case 925, 163 Avenue de Luminy, F-13288 Marseille cedex 9, France.
authors.forename: Laurie
authors.initials: L
authors.lastname: Casalot
authors.affiliation: ANDRA, Centre de Meuse/Haute-Marne, F-55290 Bure, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Vinsot
authors.affiliation: Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, UMR 235, IRD ESIL MIO, Aix-Marseille Université, case 925, 163 Avenue de Luminy, F-13288 Marseille cedex 9, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Labat
mesh_terms: D000538:Aluminum Silicates; D001482:Base Composition; D000077215:Clay; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005602:France; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010459:Peptococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016999:Sulfur-Reducing Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D000538:Aluminum Silicates; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7; D000077215:Clay