title: Ultrafine carbon black disturbs heart rate variability in mice.
abstract: Previous epidemiological and toxicological studies have reported the associations between ambient particulate matter (PM) exposure and changes in heart rate variability (HRV), a marker of cardiac autonomic nervous system (ANS) function. However, both the responsible components in PM and their mechanisms affecting HRV remain uncertain. We propose that carbon black (CB), one of the main components in PM, may affect HRV through mechanisms independent of cardio-pulmonary and systemic inflammation and/or injury. Male C57BL/6 mice were exposed by intra-tracheal instillation to ultrafine CB (once every two days for three times) at doses of 0, 0.05, 0.15 and 0.6 mg/kg. HRV indices, standard deviation of all normal R-R intervals (SDNN) and the square root of mean of sum of squares of differences between adjacent normal R-R intervals (RMSSD), showed significant decreases in 0.15 and 0.6 mg/kg CB exposed groups. Slight pulmonary inflammation and myocardial injury were only observed in 0.6 mg/kg CB exposed group. We conclude that CB can disturb cardiac ANS function in mice, indicated by the withdrawal of parasympathetic modulation, through mechanisms independent of apparent myocardial and pulmonary injury.
authors.affiliation: Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Jia
authors.forename: Yu
authors.initials: Y
authors.lastname: Hao
authors.forename: Xinbiao
authors.initials: X
authors.lastname: Guo
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000187:drug effects; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002097:C-Reactive Protein / Q000378:metabolism; D004562:Electrocardiography; D006321:Heart / Q000187:drug effects / Q000294:innervation; D006331:Heart Diseases / Q000139:chemically induced / Q000473:pathology; D006339:Heart Rate / Q000187:drug effects*; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000473:pathology; D008171:Lung Diseases / Q000139:chemically induced / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000473:pathology; D052638:Particulate Matter / Q000633:toxicity*; D053260:Soot / Q000633:toxicity*; D019210:Troponin I / Q000378:metabolism
chemical_list: D052638:Particulate Matter; D053260:Soot; D019210:Troponin I; D008315:Malondialdehyde; D002097:C-Reactive Protein; D007770:L-Lactate Dehydrogenase