title: Surface modification of PAMAM dendrimer improves its biocompatibility.
abstract: Modification of dendrimer surface groups is one of the methods available to obtain compounds characterized by reduced toxicity. This article reports results of preliminary biocompatibility studies of a modified polyamidoamine dendrimer of the fourth generation. Reaction with dimethyl itaconate resulted in transformation of surface amine groups into pyrrolidone derivatives. Interaction of the modified dendrimer with human serum albumin (HSA) was analyzed. The influence of the dendrimer on mouse neuroblastoma cell line viability and its hemolytic properties were also investigated. The binding constant between analyzed dendrimer and HSA was found to be equal to 1.2 × 10(5) ± 0.2 × 10(5) M(-1). Small changes in HSA secondary structure were observed. The analyzed dendrimer revealed minor toxic activity, as diminishment in cell viability was observed only for dendrimer concentrations higher than 2 mg/mL. Moreover, under the applied experimental conditions, no hemolytic activity was observed. Those observations point to the potential of the analyzed compound for further studies toward its applicability in nanomedicine.
authors.affiliation: Department of General Biophysics, University of Lodz, Lodz, Poland. m.ciolkowski@gmail.com
authors.forename: Michal
authors.initials: M
authors.lastname: Ciolkowski
authors.forename: Johannes F
authors.initials: JF
authors.lastname: Petersen
authors.forename: Mario
authors.initials: M
authors.lastname: Ficker
authors.forename: Anna
authors.initials: A
authors.lastname: Janaszewska
authors.forename: Jørn B
authors.initials: JB
authors.lastname: Christensen
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D050091:Dendrimers / Q000138:chemical synthesis* / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D050091:Dendrimers; C104700:PAMAM Starburst