title: Detection of dopamine in dopaminergic cell using nanoparticles-based barcode DNA analysis.
abstract: Nanotechnology-based bio-barcode-amplification analysis may be an innovative approach to dopamine detection. In this study, we evaluated the efficacy of this bio-barcode DNA method in detecting dopamine from dopaminergic cells. Herein, a combination DNA barcode and bead-based immunoassay for neurotransmitter detection with PCR-like sensitivity is described. This method relies on magnetic nanoparticles with antibodies and nanoparticles that are encoded with DNA, and antibodies that can sandwich the target protein captured by the nanoparticle-bound antibodies. The aggregate sandwich structures are magnetically separated from solution, and treated in order to remove the conjugated barcode DNA. The DNA barcodes were then identified via PCR analysis. The dopamine concentration in dopaminergic cells can be readily and rapidly detected via the bio-barcode assay method. The bio-barcode assay method is, therefore, a rapid and high-throughput screening tool for the detection of neurotransmitters such as dopamine.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, #1 Shinsu-Dong, Mapo-Gu, Seoul 121-742, Korea.
authors.forename: Jeung Hee
authors.initials: JH
authors.lastname: An
authors.forename: Tae-Hyung
authors.initials: TH
authors.lastname: Kim
authors.forename: Byung-Keun
authors.initials: BK
authors.lastname: Oh
authors.forename: Jeong Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004298:Dopamine / Q000378:metabolism*; D059290:Dopaminergic Neurons / Q000378:metabolism*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size
chemical_list: D004247:DNA; D004298:Dopamine