title: Analysis of tomato microRNAs expression profile induced by Cucumovirus and Tobamovirus infections.
abstract: MicroRNAs (miRNAs) are a class of small RNAs that affect the morphological and physiological development of plants. In recent years, there is accumulating evidence that miRNAs are involved in defense mechanism of host plants. Therefore, investigating the alteration of miRNAs expression profiles after virus infection will provide new insights for understanding the sophisticated virus-host plant interaction. The current miRNA sequence database (miRBase) contains more than 1669 mature plant miRNAs across 25 species, but few tomato miRNAs are reported. Here we created a microarray suitable for detection of plant miRNAs based on the conservative character of miRNAs, and a total of 105 conserved plant miRNAs were detected from tomato leaf tissues. Among them, 85% of the detected miRNAs showed significant expression alterations when infected by different strains of cucumber mosaic virus (CMV) and N5 strain of tomato mosaic virus (ToMV). Combination with their symptoms development, interferences of CMV 2b protein and alleviated/aggravated satellite RNA on host miRNA pathway were discussed, and the differences in interference mechanisms between CMV and ToMV on host miRNA pathway were compared. Our results represent the comprehensive investigation of tomato miRNAs on a genome scale thus far and provide information to study the interaction between plant viruses and host plants.
authors.affiliation: Institute of Bioresource Engineering, Nanjing University of Technology, Nanjing 210009, China.
authors.forename: Jishuang
authors.initials: J
authors.lastname: Chen
authors.forename: Junli
authors.initials: J
authors.lastname: Feng
authors.forename: Qiansheng
authors.initials: Q
authors.lastname: Liao
authors.forename: Shaoning
authors.initials: S
authors.lastname: Chen
authors.forename: Jianguang
authors.initials: J
authors.lastname: Zhang
authors.forename: Qiulei
authors.initials: Q
authors.lastname: Lang
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Du
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zheng
authors.forename: Pingkai
authors.initials: P
authors.lastname: Ouyang
mesh_terms: D017799:Cucumovirus / Q000502:physiology*; D018551:Solanum lycopersicum / Q000235:genetics* / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology*; D017898:Tobamovirus / Q000502:physiology*