title: Metabolic profiling of an alcoholic fatty liver in zebrafish (Danio rerio).
abstract: Zebrafish (Danio rerio) is becoming a popular developmental biology model to study diseases and for drug discovery. In this study, we performed proton nuclear magnetic resonance spectroscopy ((1)H-NMR)- and gas chromatography-mass spectrometry (GC/MS)-based metabolic profiling of an alcoholic fatty liver using a zebrafish disease model. We examined metabolic differences between the control and alcoholic fatty liver groups in zebrafish to determine how metabolism in an alcoholic fatty liver is regulated. Multivariate statistical analysis showed a significant difference between the control and alcoholic fatty liver groups. The alcoholic fatty liver group showed increased excretion of isoleucine, acetate, succinate, choline, creatine, acetoacetate, 3-hydroxybutyrate (3HB), ethyl glucuronide (EtG), lactate/pyruvate ratio, fatty acids, and cholesterol, and decreased excretion of citrate, aspartate, tyrosine, glycine, glucose, alanine, betaine, and maltose. Metabolites identified in the fatty liver groups were associated with long-term alcohol consumption, which causes both oxidation-reduction (redox) changes and oxidative stress. This study suggests that global metabolite profiling in a zebrafish model can provide insights into the metabolic changes in an alcoholic fatty liver.
authors.affiliation: Seoul Centre, Korea Basic Science Institute, Seoul 136-713, Republic of Korea.
authors.forename: Zi-Hey
authors.initials: ZH
authors.lastname: Jang
authors.forename: Hee-Chung
authors.initials: HC
authors.lastname: Chung
authors.forename: Yun Gyong
authors.initials: YG
authors.lastname: Ahn
authors.forename: Yong-Kook
authors.initials: YK
authors.lastname: Kwon
authors.forename: Jin-Sup
authors.initials: JS
authors.lastname: Kim
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Ryu
authors.forename: Do Hyun
authors.initials: DH
authors.lastname: Ryu
authors.forename: Cheol-Hee
authors.initials: CH
authors.lastname: Kim
authors.forename: Geum-Sook
authors.initials: GS
authors.lastname: Hwang
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D005227:Fatty Acids / Q000378:metabolism*; D005235:Fatty Liver, Alcoholic / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000378:metabolism*; D055442:Metabolome; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D015027:Zebrafish
chemical_list: D000596:Amino Acids; D005227:Fatty Acids