title: The use of fish-derived cell lines for investigation of environmental contaminants.
abstract: This unit describes protocols for growing salmonid cell lines and using them in in vitro toxicology studies. Cell viability of cultures is assessed with three indicator dyes: alamar blue for metabolic activity, CFDA-AM for membrane integrity, and neutral red for lysosomal activity. These protocols are essential tools for investigating environmental toxicity at the cellular level.
authors.affiliation: University of Waterloo, Waterloo, Canada.
authors.forename: Vivian R
authors.initials: VR
authors.lastname: Dayeh
authors.forename: Niels C
authors.initials: NC
authors.lastname: Bols
authors.forename: Kristin
authors.initials: K
authors.lastname: Schirmer
authors.forename: Lucy E J
authors.initials: LE
authors.lastname: Lee
mesh_terms: D023401:Animal Use Alternatives; D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000633:toxicity*; D005880:Gills / Q000166:cytology*; D008247:Lysosomes / Q000187:drug effects; D017686:Oncorhynchus mykiss; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D004785:Environmental Pollutants