title: [Virulence genes and bacteriocins in Enterococcus faecalis strains isolated from different clinical samples in Maule Region, Chile].
abstract: The presence of virulence genes (VG) and bacteriocins from different clinical samples was studied in Enterococcus faecalis isolated from urinary tract infections (UTI), bacteremia and endodontitis and was correlated with haemolysin and gelatinase activity. We evaluated the presence of VG by PCR in 150 strains of E. faecalis including cylA, aggA, efaA, eep, gelE, esp, as-48, bac31, entL50A/B, entA, entP, entB, enlA andentl071. Haemolysin and gelatinase activity was studied. gelE and cylA genes expressed hemolysin and gelatinase, respectively. This activity was observed in some strains of bacteremia, UTI and endodontitis. The highest number of VG was detected in bacteremic strains, being aggA and entA genes the most frequent. efaA, esp, entA, entL50A/B were associated with their clinical origin (p < 0.05). The most common genetic profile was aggA-eep-enlA-entL50A/B. E. faecalis from UTI, bacteremia and endodontitis presented different gene combinations. Some of the genes studied were related to their clinical origin. The results obtained in this study are similar to those reported in other countries.
authors.affiliation: Departamento de Microbiología, Universidad de Talca, Talca, Chile. cpadilla@utalca.cl
authors.forename: Carlos
authors.initials: C
authors.lastname: Padilla E
authors.forename: María
authors.initials: M
authors.lastname: Núñez A
authors.forename: Andrés
authors.initials: A
authors.lastname: Padilla G
authors.forename: Olga
authors.initials: O
authors.lastname: Lobos G
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001430:Bacteriocins / Q000235:genetics*; D002677:Chile; D013293:Enterococcus faecalis / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D005260:Female; D018093:Gelatinases / Q000096:biosynthesis / Q000235:genetics*; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D006460:Hemolysin Proteins; D037521:Virulence Factors; D018093:Gelatinases