title: Mediation of Drosophila autosomal dosage effects and compensation by network interactions.
abstract: BACKGROUND
  Gene dosage change is a mild perturbation that is a valuable tool for pathway reconstruction in Drosophila. While it is often assumed that reducing gene dose by half leads to two-fold less expression, there is partial autosomal dosage compensation in Drosophila, which may be mediated by feedback or buffering in expression networks.
  
  
  RESULTS
  We profiled expression in engineered flies where gene dose was reduced from two to one. While expression of most one-dose genes was reduced, the gene-specific dose responses were heterogeneous. Expression of two-dose genes that are first-degree neighbors of one-dose genes in novel network models also changed, and the directionality of change depended on the response of one-dose genes.
  
  
  CONCLUSIONS
  Our data indicate that expression perturbation propagates in network space. Autosomal compensation, or the lack thereof, is a gene-specific response, largely mediated by interactions with the rest of the transcriptome.
authors.affiliation: Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, 50 South Drive, Bethesda, MD 20892, USA.
authors.forename: John H
authors.initials: JH
authors.lastname: Malone
authors.forename: Dong-Yeon
authors.initials: DY
authors.lastname: Cho
authors.forename: Nicolas R
authors.initials: NR
authors.lastname: Mattiuzzo
authors.forename: Carlo G
authors.initials: CG
authors.lastname: Artieri
authors.forename: Lichun
authors.initials: L
authors.lastname: Jiang
authors.forename: Ryan K
authors.initials: RK
authors.lastname: Dale
authors.forename: Harold E
authors.initials: HE
authors.lastname: Smith
authors.forename: Jennifer
authors.initials: J
authors.lastname: McDaniel
authors.forename: Sarah
authors.initials: S
authors.lastname: Munro
authors.forename: Marc
authors.initials: M
authors.lastname: Salit
authors.forename: Justen
authors.initials: J
authors.lastname: Andrews
authors.forename: Teresa M
authors.initials: TM
authors.lastname: Przytycka
authors.forename: Brian
authors.initials: B
authors.lastname: Oliver
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D059006:Chromosomes, Insect / Q000235:genetics; D004303:Dosage Compensation, Genetic; D004330:Drosophila / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D053263:Gene Regulatory Networks; D017344:Genes, Insect; D018740:Genetic Heterogeneity; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D059467:Transcriptome; D014960:X Chromosome / Q000235:genetics