title: A proteomics performance standard to support measurement quality in proteomics.
abstract: The emergence of MS-based proteomic platforms as a prominent technology utilized in biochemical and biomedical research has increased the need for high-quality MS measurements. To address this need, National Institute of Standards and Technology (NIST) reference material (RM) 8323 yeast protein extract is introduced as a proteomics quality control material for benchmarking the preanalytical and analytical performance of proteomics-based experimental workflows. RM 8323 yeast protein extract is based upon the well-characterized eukaryote Saccharomyces cerevisiae and can be utilized in the design and optimization of proteomics-based methodologies from sample preparation to data analysis. To demonstrate its utility as a proteomics quality control material, we coupled LC-MS/MS measurements of RM 8323 with the NIST MS Quality Control (MSQC) performance metrics to quantitatively assess the LC-MS/MS instrumentation parameters that influence measurement accuracy, repeatability, and reproducibility. Due to the complexity of the yeast proteome, we also demonstrate how NIST RM 8323, along with the NIST MSQC performance metrics, can be used in the evaluation and optimization of proteomics-based sample preparation methods.
authors.affiliation: Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD, USA. ashley.beasley@nist.gov
authors.forename: Ashley
authors.initials: A
authors.lastname: Beasley-Green
authors.forename: David
authors.initials: D
authors.lastname: Bunk
authors.forename: Paul
authors.initials: P
authors.lastname: Rudnick
authors.forename: Lisa
authors.initials: L
authors.lastname: Kilpatrick
authors.forename: Karen
authors.initials: K
authors.lastname: Phinney
mesh_terms: D002853:Chromatography, Liquid / Q000592:standards; D005656:Fungal Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods* / Q000592:standards*; D012015:Reference Standards; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae; D053719:Tandem Mass Spectrometry / Q000592:standards
chemical_list: D005656:Fungal Proteins; D020543:Proteome