title: Cryopreservation of collared peccaries (Tayassu tajacu) semen using a powdered coconut water (ACP-116c) based extender plus various concentrations of egg yolk and glycerol.
abstract: The objective was to determine the effectiveness of a powdered coconut water-based extender (ACP-116c), plus various concentrations of egg-yolk and glycerol, as an alternative for cryopreservation of collared peccary semen. Twelve ejaculates were obtained from captive adult males by electroejaculation, and evaluated for sperm motility, kinetic rating, viability, morphology, and functional membrane integrity. The ejaculates were apportioned into aliquots that were diluted in Tris plus 10% egg yolk and 3% glycerol, or in ACP-116c plus 10 or 20% egg yolk and 1.5 or 3% glycerol. Samples were frozen in liquid nitrogen and, after 1 mo, thawed at 37 °C for 1 min. After thawing, samples were evaluated as reported for fresh semen, and also for sperm membrane integrity (fluorescent probes) and kinematic parameters (computerized analysis). Results were presented as means ± SEM. Freezing and thawing decreased sperm characteristics relative to fresh semen. Overall, ACP-116c plus 20% egg yolk and 3% glycerol provided better (P < 0.05) sperm motility and kinetic rating (48 ± 6.1% and 2.8 ± 0.2, respectively) after thawing than Tris extender (30.4 ± 5.7% and 2.4 ± 0.2). However, there were no differences (P > 0.05) among treatments with regard to the other sperm characteristics. Based on computerized motion analysis, total (26.5 ± 5.9%) and progressive (8.1 ± 2.2%) motility were best preserved (P < 0.05) with the above-mentioned treatment. In conclusion, a coconut water-based extender, ACP-116c, plus 20% egg yolk and 3% glycerol, was effective for cryopreservation of semen from collared peccaries.
authors.affiliation: Laboratory of Animal Germplasm Conservation, LCGA, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Silva
authors.forename: G C X
authors.initials: GC
authors.lastname: Peixoto
authors.forename: G L
authors.initials: GL
authors.lastname: Lima
authors.forename: J A B
authors.initials: JA
authors.lastname: Bezerra
authors.forename: L B
authors.initials: LB
authors.lastname: Campos
authors.forename: A L C
authors.initials: AL
authors.lastname: Paiva
authors.forename: V V
authors.initials: VV
authors.lastname: Paula
authors.forename: A R
authors.initials: AR
authors.lastname: Silva
mesh_terms: D000818:Animals; D001188:Artiodactyla; D002462:Cell Membrane / Q000648:ultrastructure; D003059:Cocos; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents; D004530:Egg Yolk; D005990:Glycerol; D006358:Hot Temperature; D008297:Male; D055101:Semen Analysis / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D003451:Cryoprotective Agents; D005990:Glycerol