title: A dimethoxytriazine type glycosyl donor enables a facile chemo-enzymatic route toward α-linked N-acetylglucosaminyl-galactose disaccharide unit from gastric mucin.
abstract: An efficient chemo-enzymatic process for construction of the α-linked disaccharide unit (GlcNAcα1-4Gal) found in gastric mucin has been developed. The process consists of a one-step preparation of a novel triazine type glycosyl donor in water and the subsequent transglycosylation to a galactose derivative catalysed by α-N-acetylglucosaminidase.
authors.affiliation: Department of Biomolecular Engineering, Tohoku University, 6-6-11-514 Aoba, Sendai 980-8579, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Noguchi
authors.forename: Miwa
authors.initials: M
authors.lastname: Nakamura
authors.forename: Ayaka
authors.initials: A
authors.lastname: Ohno
authors.forename: Tomonari
authors.initials: T
authors.lastname: Tanaka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Masaki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Masaya
authors.initials: M
authors.lastname: Fujita
authors.forename: Akiko
authors.initials: A
authors.lastname: Tsuchida
authors.forename: Mamoru
authors.initials: M
authors.lastname: Mizuno
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Shoda
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D001439:Bacteroides / Q000201:enzymology; D004187:Disaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D005690:Galactose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D005752:Gastric Mucins / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D014227:Triazines / Q000737:chemistry / Q000378:metabolism
chemical_list: D004187:Disaccharides; D005752:Gastric Mucins; D014227:Triazines; C033079:alpha-N-acetyl-D-glucosaminidase; D000118:Acetylglucosaminidase; D005690:Galactose