title: Cardiac myosin binding protein C and MAP-kinase activating death domain-containing gene polymorphisms and diastolic heart failure.
abstract: OBJECTIVE
  Myosin binding protein C (MYBPC3) plays a role in ventricular relaxation. The aim of the study was to investigate the association between cardiac myosin binding protein C (MYBPC3) gene polymorphisms and diastolic heart failure (DHF) in a human case-control study.
  
  
  METHODS
  A total of 352 participants of 1752 consecutive patients from the National Taiwan University Hospital and its affiliated hospital were enrolled. 176 patients diagnosed with DHF confirmed by echocardiography were recruited. Controls were matched 1-to-1 by age, sex, hypertension, diabetes, renal function and medication use. We genotyped 12 single nucleotide polymorphisms (SNPs) according to HapMap Han Chinese Beijing databank across a 40 kb genetic region containing the MYBPC3 gene and the neighboring DNA sequences to capture 100% of haplotype variance in all SNPs with minor allele frequencies ≥ 5%. We also analyzed associations of these tagging SNPs and haplotypes with DHF and linkage disequilibrium (LD) structure of the MYBPC3 gene.
  
  
  RESULTS
  In a single locus analysis, SNP rs2290149 was associated with DHF (allele-specific p = 0.004; permuted p = 0.031). The SNP with a minor allele frequency of 9.4%, had an odds ratio 2.14 (95% CI 1.25-3.66; p = 0.004) for the additive model and 2.06 for the autosomal dominant model (GG+GA : AA, 95% CI 1.17-3.63; p = 0.013), corresponding to a population attributable risk fraction of 12.02%. The haplotypes in a LD block of rs2290149 (C-C-G-C) was also significantly associated with DHF (odds ratio 2.10 (1.53-2.89); permuted p = 0.029).
  
  
  CONCLUSIONS
  We identified a SNP (rs2290149) among the tagging SNP set that was significantly associated with early DHF in a Chinese population.
authors.affiliation: Division of Cardiology, Department of Internal Medicine, National Taiwan University College of Medicine and Hospital, Taipei, Taiwan.
authors.forename: Cho-Kai
authors.initials: CK
authors.lastname: Wu
authors.forename: Yin-Tsen
authors.initials: YT
authors.lastname: Huang
authors.forename: Jen-Kuang
authors.initials: JK
authors.lastname: Lee
authors.forename: Liang-Ting
authors.initials: LT
authors.lastname: Chiang
authors.forename: Fu-Tien
authors.initials: FT
authors.lastname: Chiang
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Huang
authors.forename: Jiunn-Lee
authors.initials: JL
authors.lastname: Lin
authors.forename: Chuen-Den
authors.initials: CD
authors.lastname: Tseng
authors.forename: Yau-Hung
authors.initials: YH
authors.lastname: Chen
authors.forename: Chia-Ti
authors.initials: CT
authors.lastname: Tsai
mesh_terms: D000368:Aged; D002352:Carrier Proteins / Q000235:genetics*; D016022:Case-Control Studies; D053418:Death Domain Receptor Signaling Adaptor Proteins / Q000235:genetics*; D004452:Echocardiography; D005260:Female; D017353:Gene Deletion; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics*; D006239:Haplotypes; D054144:Heart Failure, Diastolic / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D000099163:Myosin Binding Protein C
chemical_list: D002352:Carrier Proteins; D053418:Death Domain Receptor Signaling Adaptor Proteins; D020662:Guanine Nucleotide Exchange Factors; D000099163:Myosin Binding Protein C; C105852:MADD protein, human