title: Envisioning an enzymatic Diels-Alder reaction by in situ acid-base catalyzed diene generation.
abstract: We present and evaluate a new and potentially efficient route for enzyme-mediated Diels-Alder reactions, utilizing general acid-base catalysis. The viability of employing the active site of ketosteroid isomerase is demonstrated.
authors.affiliation: Applied Physical Chemistry, KTH Royal Institute of Technology, Teknikringen 30-36, S-10044 Stockholm, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Linder
authors.forename: Adam Johannes
authors.initials: AJ
authors.lastname: Johansson
authors.forename: Bianca
authors.initials: B
authors.lastname: Manta
authors.forename: Philip
authors.initials: P
authors.lastname: Olsson
authors.forename: Tore
authors.initials: T
authors.lastname: Brinck
mesh_terms: D000143:Acids / Q000737:chemistry; D000466:Alkadienes / Q000138:chemical synthesis* / Q000737:chemistry; D002384:Catalysis; D020134:Catalytic Domain; D007659:Ketones / Q000138:chemical synthesis / Q000737:chemistry; D008958:Models, Molecular; D011522:Protons; D011549:Pseudomonas / Q000737:chemistry / Q000201:enzymology*; D013237:Stereoisomerism; D013251:Steroid Isomerases / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D000143:Acids; D000466:Alkadienes; D007659:Ketones; D011522:Protons; D013251:Steroid Isomerases