title: The effects of chronic copper exposure on the amyloid protein metabolisim associated genes' expression in chronic cerebral hypoperfused rats.
abstract: The pathogens of Alzheimer's disease (AD) are still unclear, while accumulating evidences have indicated that both genetic and environmental factors are involved in the pathogenesis of AD. Recent studies suggest that AD is primarily a vascular disorder and copper (Cu) may play an important role in AD pathology. However, the consequences of chronic Cu exposure at the presence of other AD risk factors remain to be clarified. To investigate the effects of chronic Cu intake on cerebral hypoperfusion-induced AD pathology, Sprague-Dawley rats suffered bilateral common carotid artery occlusion (2VO) were administrated with 250 ppm copper-containing water or not. Morris water maze test showed that Cu exposure for 3 months exacerbated cognitive impairment induced by 2VO. Elevated amyloid precursor protein (APP) and beta-site APP-cleaving enzyme 1 (BACE1) expression in mRNA and protein levels were also observed in brain of Cu-exposed rats suffered 2VO. In contrast, these Cu-exacerbated changes were ameliorated after Cu was withdrawn from drinking water. In summary, our findings demonstrate that chronic Cu exposure might exacerbate AD pathology in 2VO rats.
authors.affiliation: Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China.
authors.forename: Xuexuan
authors.initials: X
authors.lastname: Mao
authors.forename: Jiantao
authors.initials: J
authors.lastname: Ye
authors.forename: Shiyou
authors.initials: S
authors.lastname: Zhou
authors.forename: Rongbiao
authors.initials: R
authors.lastname: Pi
authors.forename: Juan
authors.initials: J
authors.lastname: Dou
authors.forename: Linquan
authors.initials: L
authors.lastname: Zang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Chao
authors.forename: Wenming
authors.initials: W
authors.lastname: Li
authors.forename: Mengfei
authors.initials: M
authors.lastname: Liu
authors.forename: Peiqing
authors.initials: P
authors.lastname: Liu
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002561:Cerebrovascular Disorders / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D003072:Cognition Disorders / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D003300:Copper / Q000633:toxicity*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects*; D018782:Maze Learning / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D000682:Amyloid; D003300:Copper