title: The immunoexpression of Shh, Smo and Gli2 in Helicobacter pylori positive and negative gastric biopsies.
abstract: The Hedgehog signaling pathway plays a principal role during embryonic development, tissue regeneration and carcinogenesis in various adult tissues. Although hedgehog signaling is important in gastric carcinogenesis, its role in Helicobacter pylori-associated gastritis is unclear. The aim of our study was to examine Sonic Hedgehog (Shh) signaling pathway in response to H. pylori infection. Thirty-one formalin-fixed, paraffin-embedded tissue specimens of chronic gastritis were retrieved from archival material. The immunoexpression of Shh, Smoothened (Smo) and Glioblastoma transcription factor 2 (Gli2) were detected using the immunohistochemical method. Sonic Hedgehog protein was expressed in H. pylori-positive and H. pylori-negative groups of patients. The immunoexpression of Shh, Smo and Gli2 proteins was lower in H. pylori-positive group compared to H. pylori-negative group, however only the differences in Shh and Smo immunoexpression were statistically significant. The immunoexpression of Shh was significantly correlated with the immunoexpression of Smo in both tested groups (p < 0.001, p < 0.02, respectively). No statistically significant correlation was found between Shh and Gli2 among H. pylori-positive and H. pylori-negative groups. The above findings support the hypothesis of the involvement of Shh signaling pathway in H. Pylori-associated gastritis.
authors.affiliation: Department of Nefropathology, Medical University of Lodz, Poland. olga.stasikowska@umed.lodz.pl
authors.forename: Olga
authors.initials: O
authors.lastname: Stasikowska-Kanicka
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wągrowska-Danilewicz
authors.forename: Iwona
authors.initials: I
authors.lastname: Białek
authors.forename: Marian
authors.initials: M
authors.lastname: Danilewicz
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D005757:Gastritis, Atrophic / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D053823:Hedgehog Proteins / Q000096:biosynthesis*; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis*; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis*; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis*; D000072086:Smoothened Receptor; D000075746:Zinc Finger Protein Gli2
chemical_list: C082593:GLI2 protein, human; D053823:Hedgehog Proteins; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins; D043562:Receptors, G-Protein-Coupled; C509239:SHH protein, human; C491023:SMO protein, human; D000072086:Smoothened Receptor; D000075746:Zinc Finger Protein Gli2