title: A proteogenomic approach to map the proteome of an unsequenced pathogen - Leishmania donovani.
abstract: Visceral leishmaniasis or kala azar is the most severe form of leishmaniasis and is caused by the protozoan parasite Leishmania donovani. There is no published report on L. donovani genome sequence available till date, although the genome sequences of three related Leishmania species are already available. Thus, we took a proteogenomic approach to identify proteins from two different life stages of L. donovani. From our analysis of the promastigote (insect) and amastigote (human) stages of L. donovani, we identified a total of 22,322 unique peptides from a homology-based search against proteins from three Leishmania species. These peptides were assigned to 3711 proteins in L. infantum, 3287 proteins in L. major, and 2433 proteins in L. braziliensis. Of the 3711 L. donovani proteins that were identified, the expression of 1387 proteins was detectable in both life stages of the parasite, while 901 and 1423 proteins were identified only in promastigotes and amastigotes life stages, respectively. In addition, we also identified 13 N-terminally and one C-terminally extended proteins based on the proteomic data search against the six-frame translated genome of the three related Leishmania species. Here, we report results from proteomic profiling of L. donovani, an organism with an unsequenced genome.
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore, Karnataka, India.
authors.forename: Harsh
authors.initials: H
authors.lastname: Pawar
authors.forename: Nandini A
authors.initials: NA
authors.lastname: Sahasrabuddhe
authors.forename: Santosh
authors.initials: S
authors.lastname: Renuse
authors.forename: Shivakumar
authors.initials: S
authors.lastname: Keerthikumar
authors.forename: Jyoti
authors.initials: J
authors.lastname: Sharma
authors.forename: Ghantasala S Sameer
authors.initials: GS
authors.lastname: Kumar
authors.forename: Abhilash
authors.initials: A
authors.lastname: Venugopal
authors.forename: Nirujogi Raja
authors.initials: NR
authors.lastname: Sekhar
authors.forename: Dhanashree S
authors.initials: DS
authors.lastname: Kelkar
authors.forename: Harshal
authors.initials: H
authors.lastname: Nemade
authors.forename: Sweta N
authors.initials: SN
authors.lastname: Khobragade
authors.forename: Babylakshmi
authors.initials: B
authors.lastname: Muthusamy
authors.forename: Kumaran
authors.initials: K
authors.lastname: Kandasamy
authors.forename: H C
authors.initials: HC
authors.lastname: Harsha
authors.forename: Raghothama
authors.initials: R
authors.lastname: Chaerkady
authors.forename: Milind S
authors.initials: MS
authors.lastname: Patole
authors.forename: Akhilesh
authors.initials: A
authors.lastname: Pandey
mesh_terms: D000595:Amino Acid Sequence; D030562:Databases, Protein; D007893:Leishmania donovani / Q000737:chemistry* / Q000235:genetics; D007898:Leishmaniasis, Visceral / Q000382:microbiology; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D015800:Protozoan Proteins / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry; D037521:Virulence Factors / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification
chemical_list: D020543:Proteome; D015800:Protozoan Proteins; D037521:Virulence Factors