title: Human herpesvirus 6 glycoprotein M is essential for virus growth and requires glycoprotein N for its maturation.
abstract: Human herpesvirus 6 (HHV-6) is a T-lymphotropic virus belonging to the betaherpesvirus family. Several HHV-6-encoded glycoproteins are required for cell entry and virion maturation. Glycoprotein M (gM) is conserved among all herpesviruses, and therefore thought to have important functions; however, the HHV-6 g has not been characterized. Here, we examined the expression of HHV-6 g, and examined its function in viral replication, using a mutant and revertant gM. HHV-6 g was expressed on virions as a glycoprotein modified with complex N-linked oligosaccharides. As in other herpesviruses, HHV-6 g formed a complex with glycoprotein N (gN), and was transported from the endoplasmic reticulum to the trans-Golgi network only when part of this complex. Finally, a gM mutant virus in which the gM start codon was destroyed was not reconstituted, although its revertant was, indicating that HHV-6 g is essential for virus production, unlike the gM of alphaherpesviruses.
authors.affiliation: Division of Clinical Virology, Kobe University Graduate School of Medicine, Chuo-ku, Kobe, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kawabata
authors.forename: Chyntia
authors.initials: C
authors.lastname: Jasirwan
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamanishi
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Mori
mesh_terms: D002460:Cell Line; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D006801:Humans; D021381:Protein Transport; D019349:Roseolovirus Infections / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D021601:trans-Golgi Network / Q000821:virology
chemical_list: D014759:Viral Envelope Proteins