title: Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation).
abstract: We have developed a new multimodal molecular imaging system that combines CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic generation), THG (third harmonic generation) and multiplex TSFG (third-order sum frequency generation) using a subnanosecond white-light laser source. Molecular composition and their distribution in living cells are clearly visualized with different contrast enhancements through different mechanisms of CARS, SHG, THG and TSFG. A correlation image of CARS and TSF reveals that the TSF signal is generated predominantly from lipid droplets inside a cell as well as the peripheral cell wall.
authors.affiliation: Department of Chemistry, School of Science, The University of Tokyo, Hongo, 7-3-1, Bunkyo, Tokyo,113-0033, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Segawa
authors.forename: Masanari
authors.initials: M
authors.lastname: Okuno
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kano
authors.forename: Philippe
authors.initials: P
authors.lastname: Leproux
authors.forename: Vincent
authors.initials: V
authors.lastname: Couderc
authors.forename: Hiro-O
authors.initials: HO
authors.lastname: Hamaguchi
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D057054:Molecular Imaging / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D013194:Staining and Labeling; D015003:Yeasts / Q000166:cytology*