title: shRNA-Mediated BAALC knockdown affects proliferation and apoptosis in human acute myeloid leukemia cells.
abstract: Brain and acute leukemia, cytoplasmic (BAALC) is a novel molecular marker indicating an inferior outcome in acute myeloid leukemia (AML) with normal cytogenetics. The biological function of BAALC is largely unknown. In this study, BAALC gene expression in an acute myeloid leukemia cell  line KG1a was knocked down by a small hairpin RNA (shRNA). The expression of BAALC mRNA and protein in the knockdown cells was significantly inhibited. The proliferation and apoptosis status in the knockdown cells were investigated. The growth curves and FACS analysis demonstrated that BAALC  gene knockdown resulted in decreased proliferation and enhanced apoptosis in KG1a cells. These results indicated that BAALC may act as an adverse prognostic factor through prompting proliferation and inhibiting apoptosis in leukemia cells.
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
authors.forename: Guoshu
authors.initials: G
authors.lastname: Chen
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Shi
authors.forename: Xutao
authors.initials: X
authors.lastname: Guo
authors.forename: Pingnan
authors.initials: P
authors.lastname: Xiao
authors.forename: Weiguang
authors.initials: W
authors.lastname: Wang
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics*; D014162:Transfection
chemical_list: C442828:BAALC protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering