title: Effects of 837 and 1950 MHz radiofrequency radiation exposure alone or combined on oxidative stress in MCF10A cells.
abstract: The aim of this study was to determine whether the exposure to either single or multiple radio-frequency (RF) radiation frequencies could induce oxidative stress in cell cultures. Exposures of human MCF10A mammary epithelial cells to either a single frequency (837 MHz alone or 1950 MHz alone) or multiple frequencies (837 and 1950 MHz) were conducted at specific absorption rate (SAR) values of 4 W/kg for 2 h. During the exposure period, the temperature in the exposure chamber was maintained isothermally. Intracellular levels of reactive oxygen species (ROS), the antioxidant enzyme activity of superoxide dismutase (SOD), and the ratio of reduced/oxidized glutathione (GSH/GSSG) showed no statistically significant alterations as the result of either single or multiple RF radiation exposures. In contrast, ionizing radiation-exposed cells, used as a positive control, showed evident changes in all measured biological endpoints. These results indicate that single or multiple RF radiation exposure did not elicit oxidative stress in MCF10A cells under our exposure conditions.
authors.affiliation: Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul, South Korea.
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Hong
authors.forename: Bong-Cho
authors.initials: BC
authors.lastname: Kim
authors.forename: Young-Gyu
authors.initials: YG
authors.lastname: Ko
authors.forename: Yun-Sil
authors.initials: YS
authors.lastname: Lee
authors.forename: Seung-Cheol
authors.initials: SC
authors.lastname: Hong
authors.forename: Taehong
authors.initials: T
authors.lastname: Kim
authors.forename: Jeong-Ki
authors.initials: JK
authors.lastname: Pack
authors.forename: Hyung-Do
authors.initials: HD
authors.lastname: Choi
authors.forename: Nam
authors.initials: N
authors.lastname: Kim
authors.forename: Jae-Seon
authors.initials: JS
authors.lastname: Lee
mesh_terms: D045744:Cell Line, Tumor; D019803:Glutathione Disulfide / Q000378:metabolism; D006801:Humans; D018384:Oxidative Stress / Q000528:radiation effects*; D011846:Radio Waves / Q000009:adverse effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D019803:Glutathione Disulfide