title: A low prevalence of mycobacteria in freshwater fish from water reservoirs, ponds and farms.
abstract: A survey of the occurrence of mycobacteria was conducted from 717 freshwater fish (25 species) in two water reservoirs, five ponds and two farms in the Czech Republic. A total of 2182 tissue samples from these fish were examined using the conventional culture method. Thirteen mycobacterial isolates were obtained from 12 (1.7%) fish belonging to nine species. Isolates were identified using sequence analysis of the 16SrRNA gene as: Mycobacterium algericum, M. fortuitum, M. gordonae, M. insubricum, M. kumamotonense, M. nonchromogenicum, two isolates of M. peregrinum, M. terrae and M. triplex. Mycobacteria were isolated more frequently from fish skin and gills than from internal organs or muscles.
authors.affiliation: Veterinary Research Institute, Brno, v.v.i., Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Mrlik
authors.forename: M
authors.initials: M
authors.lastname: Slany
authors.forename: J
authors.initials: J
authors.lastname: Kubecka
authors.forename: J
authors.initials: J
authors.lastname: Seda
authors.forename: A
authors.initials: A
authors.lastname: Necas
authors.forename: V
authors.initials: V
authors.lastname: Babak
authors.forename: I
authors.initials: I
authors.lastname: Slana
authors.forename: P
authors.initials: P
authors.lastname: Kriz
authors.forename: I
authors.initials: I
authors.lastname: Pavlik
mesh_terms: D000818:Animals; D018153:Czech Republic; D005393:Fish Diseases / Q000453:epidemiology* / Q000382:microbiology; D005399:Fishes; D005618:Fresh Water; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000453:epidemiology / Q000662:veterinary*; D061005:Ponds; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S