title: Morphology effects on the biofunctionalization of nanostructured ZnO.
abstract: A stepwise surface functionalization methodology was applied to nanostructured ZnO films grown by metal organic chemical vapor deposition (MOCVD) having three different surface morphologies (i.e., nanorod layers (ZnO films-N), rough surface films (ZnO films-R), and planar surface films (ZnO films-P). The films were grown on glass substrates and on the sensing area of a quartz crystal microbalance (nano-QCM). 16-(2-Pyridyldithiol)-hexadecanoic acid (PDHA) was bound to ZnO films-N, -R, and -P through the carboxylic acid unit, followed by a nucleophilic displacement of the 2-pyridyldithiol moiety by single-stranded DNA capped with a thiol group (SH-ssDNA). The resulting ssDNA-functionalized films were hybridized with complementary ssDNA tagged with fluorescein (ssDNA-Fl). In a selectivity control experiment, no hybridization occurred upon treatment with non complementary DNA. The ZnO films' surface functionalization, characterized by FT-IR-ATR and fluorescence spectroscopy and detected on the nano-QCM, was successful on films-N and -R but was barely detectable on the planar surface of films-P.
authors.affiliation: Department of Chemistry, Rutgers, The State University of New Jersey, Newark, New Jersey 07102, United States.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.forename: Elena
authors.initials: E
authors.lastname: Galoppini
authors.forename: Pavel Ivanoff
authors.initials: PI
authors.lastname: Reyes
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Duan
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Lu
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry*; D008567:Membranes, Artificial; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D019308:Palmitic Acid / Q000737:chemistry; D010316:Particle Size; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D008567:Membranes, Artificial; D013438:Sulfhydryl Compounds; D019308:Palmitic Acid; D015034:Zinc Oxide