title: The state of the guanosine nucleotide allosterically affects the interfaces of tubulin in protofilament.
abstract: The dynamics of microtubules is essential for many microtubule-dependent cellular functions such as the mitosis. It has been recognized for a long time that GTP hydrolysis in αβ-tubulin polymers plays a critical role in this dynamics. However, the effects of the changes in the nature of the guanosine nucleotide at the E-site in β-tubulin on microtubule structure and stability are still not well understood. In the present work, we performed all-atom molecular dynamics simulations of a αβα-tubulin heterotrimer harboring a guanosine nucleotide in three different states at the E-site: GTP, GDP-Pi and GDP. We found that changes in the nucleotide state is associated with significant conformational variations at the α-tubulin N- and β-tubulin M-loops which impact the interactions between tubulin protofilaments. The results also show that GTP hydrolysis reduces αβ-tubulin interdimer contacts in favor of intradimer interface. From an atomistic point view, we propose a role for α-tubulin glutamate residue 254 in catalytic magnesium coordination and identified a water molecule in the nucleotide binding pocket which is most probably required for nucleotide hydrolysis. Finally, the results are discussed with reference to the role of taxol in microtubule stability and the recent tubulin-sT2R crystal structures.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), UMRS 829, Evry, France.
authors.forename: Joseph R
authors.initials: JR
authors.lastname: André
authors.forename: Marie-Jeanne
authors.initials: MJ
authors.lastname: Clément
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Adjadj
authors.forename: Flavio
authors.initials: F
authors.lastname: Toma
authors.forename: Patrick A
authors.initials: PA
authors.lastname: Curmi
authors.forename: Philippe
authors.initials: P
authors.lastname: Manivet
mesh_terms: D000494:Allosteric Regulation; D006150:Guanine Nucleotides / Q000378:metabolism*; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D014404:Tubulin / Q000378:metabolism*
chemical_list: D006150:Guanine Nucleotides; D014404:Tubulin