title: Expression profile of HSP genes during different seasons in goats (Capra hircus).
abstract: The present study has demonstrated the expression of HSP60, HSP70, HSP90, and UBQ in peripheral blood mononuclear cells (PBMCs) during different seasons in three different age groups (Groups I, II, and III with age of 0-2, 2-5, and >5 years, respectively) of goats of tropical and temperate regions. Real-time polymerase chain reaction was applied to investigate mRNA expression of examined factors. Specificity of the desired products was documented using analysis of the melting temperature and high-resolution gel electrophoresis to verify that the transcripts are of the exact molecular size predicted. The mRNA expression of HSP60, HSP90, and UBQ was significantly higher (P < 0.05) in all age groups during peak summer season as compared with peak winter season in both tropical and temperate region goats. HSP70 mRNA expression was significantly higher (P < 0.05) during summer season as compared with winter season in tropical region goats. However, in the temperate region, in goats from all the three age groups studied, a non-significant difference of HSP70 expression between summer and winter seasons was noticed. In conclusion, results demonstrate that (1) HSP genes are expressed in caprine PBMCs and (2) higher expression of HSPs during thermal stress suggest possible involvement of them to ameliorate deleterious effect of thermal stress so as to maintain cellular integrity and homeostasis in goats.
authors.affiliation: Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
authors.forename: Satyaveer Singh
authors.initials: SS
authors.lastname: Dangi
authors.forename: Mahesh
authors.initials: M
authors.lastname: Gupta
authors.forename: Divakar
authors.initials: D
authors.lastname: Maurya
authors.forename: Vijay Prakash
authors.initials: VP
authors.lastname: Yadav
authors.forename: Rudra Prasanna
authors.initials: RP
authors.lastname: Panda
authors.forename: Gyanendra
authors.initials: G
authors.lastname: Singh
authors.forename: Nitai Haridas
authors.initials: NH
authors.lastname: Mohan
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Bhure
authors.forename: Bikash Chandra
authors.initials: BC
authors.lastname: Das
authors.forename: Sadhan
authors.initials: S
authors.lastname: Bag
authors.forename: Ramkrishna
authors.initials: R
authors.lastname: Mahapatra
authors.forename: Guttalu
authors.initials: G
authors.lastname: Taru Sharma
authors.forename: Mihir
authors.initials: M
authors.lastname: Sarkar
mesh_terms: D000367:Age Factors; D000818:Animals; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D006041:Goats / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D018869:Heat-Shock Response; D006358:Hot Temperature / Q000009:adverse effects; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012621:Seasons; D013045:Species Specificity; D014329:Tropical Climate; D025801:Ubiquitin / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics
chemical_list: D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D025801:Ubiquitin