title: Brevibacterium siliguriense sp. nov., a facultatively oligotrophic bacterium isolated from river water.
abstract: A Gram-positive-staining, rod-shaped, facultatively oligotrophic bacterial strain, designated MB18(T), was isolated from a water sample collected from the River Mahananda at Siliguri (26° 44' 23.20' N, 88° 25' 22.89' E), West-Bengal, India. On the basis of 16S rRNA gene sequence similarity, the closest relative of this strain was Brevibacterium epidermidis NCDO 2286(T) (96 % similarity). The DNA G+C content of strain MB18(T) was 64.6 mol%. Chemotaxonomic data [MK-8(H(2)) as the major menaquinone, galactose as the sole cell-wall sugar, meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, phosphatidylglycerol and diphosphatidylglycerol as constituents of the polar lipids, anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(15 : 0) as the major fatty acids] supported the affiliation of strain MB18(T) to the genus Brevibacterium. The results of DNA G+C content, 16S rRNA gene sequence analysis and biochemical and physiological analyses allowed genotypic and phenotypic differentiation of strain MB18(T) from its nearest neighbour B. epidermidis. The isolate therefore represents a novel species, for which the name Brevibacterium siliguriense sp. nov. is proposed; the type strain is MB18(T) ( = DSM 23676(T) = LMG 25772(T)).
authors.affiliation: Omics Laboratory, Department of Biotechnology, University of North Bengal, Raja Rammohunpur P.O. NBU, Siliguri-734013, West Bengal, India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Yeditepe University, Department of Genetics and Bioengineering, 34755 Istanbul, Turkey.|Giresun University, Faculty of Arts and Sciences, Department of Biology, 28049, Giresun, Turkey.|Wageningen University and Research Center, Laboratory of Virology, Radix Building 107, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: İkbal Agah
authors.initials: İA
authors.lastname: İnce
authors.affiliation: Yeditepe University, Department of Genetics and Bioengineering, 34755 Istanbul, Turkey.|Giresun University, Faculty of Arts and Sciences, Department of Biology, 28049, Giresun, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Katı
authors.affiliation: Omics Laboratory, Department of Biotechnology, University of North Bengal, Raja Rammohunpur P.O. NBU, Siliguri-734013, West Bengal, India.
authors.forename: Ranadhir
authors.initials: R
authors.lastname: Chakraborty
mesh_terms: D000599:Amino Acids, Diamino / Q000032:analysis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001951:Brevibacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D000599:Amino Acids, Diamino; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8