title: Comparative secretome analysis of human follicular dermal papilla cells and fibroblasts using shotgun proteomics.
abstract: The dermal papilla cells (DPCs) of hair follicles are known to secrete paracrine factors for follicular cells. Shotgun proteomic analysis was performed to compare the expression profiles of the secretomes of human DPCs and dermal fibroblasts (DFs). In this study, the proteins secreted by DPCs and matched DFs were analyzed by 1DE/LTQ FTICR MS/MS, semi-quantitatively determined using emPAI mole percent values and then characterized using protein interaction network analysis. Among the 1,271 and 1,188 proteins identified in DFs and DPCs, respectively, 1,529 were further analyzed using the Ingenuity Pathway Analysis tool. We identified 28 DPC-specific extracellular matrix proteins including transporters (ECM1, A2M), enzymes (LOX, PON2), and peptidases (C3, C1R). The biochemically- validated DPC-specific proteins included thrombospondin 1 (THBS1), an insulin-like growth factor binding protein3 (IGFBP3), and, of particular interest, an integrin beta1 subunit (ITGB1) as a key network core protein. Using the shotgun proteomic technique and network analysis, we selected ITGB1, IGFBP3, and THBS1 as being possible hair-growth modulating protein biomarkers.
authors.affiliation: Department of Dermatology, College of Medicine, Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea.
authors.forename: Chong Hyun
authors.initials: CH
authors.lastname: Won
authors.forename: Oh Sang
authors.initials: OS
authors.lastname: Kwon
authors.forename: Yong Jung
authors.initials: YJ
authors.lastname: Kang
authors.forename: Hyeon Gyeong
authors.initials: HG
authors.lastname: Yoo
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: Lee
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Kyu Han
authors.initials: KH
authors.lastname: Kim
authors.forename: Won Seok
authors.initials: WS
authors.lastname: Park
authors.forename: Nok Hyun
authors.initials: NH
authors.lastname: Park
authors.forename: Kun
authors.initials: K
authors.lastname: Cho
authors.forename: Sang Oh
authors.initials: SO
authors.lastname: Kwon
authors.forename: Jong-Soon
authors.initials: JS
authors.lastname: Choi
authors.forename: Hee Chul
authors.initials: HC
authors.lastname: Eun
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D015153:Blotting, Western; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D060066:Protein Interaction Maps; D040901:Proteomics; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers