title: [Increase of choroidal and scleral MCP-1 expression in hypercholesterolemia experimental model].
abstract: OBJECTIVE
  The aim of this study is to experientially demonstrate that a cholesterol-enriched diet induces an increase in the MCP-1 expression in the choroid and sclera.
  
  
  METHODS
  New Zealand rabbits were divided into two groups: GN (normal diet group) of 8 rabbits (8 eyes) was fed a standard rabbit diet for 4 weeks; GH (hypercholesterolemic group) of 13 rabbits (13 eyes) was fed a 1% cholesterol enriched diet for 8 weeks. Total serum cholesterol, triglyceride, HDL cholesterol and fasting blood glucose exams were performed at the start of the experiment and at the euthanasia time. After GH 8th week and GN 4th week animals were euthanized and their eyes underwent immunohistochemical analysis with the anti-MCP-1 antibody.
  
  
  RESULTS
  The diet has induced a significant increase in GH total cholesterol and triglyceride levels when compared with NG (p<0.001). There was a significant increase in the MCP-1 expression in GH choroid and sclera in relation to GN (p<0.001).
  
  
  CONCLUSIONS
  This study has revealed that the hypercholesterolemic diet in rabbits induces an increase in the MCP-1 expression in the choroid and sclera.
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil. rjat@terra.com.br
authors.forename: Rogil José de Almeida
authors.initials: RJ
authors.lastname: Torres
authors.forename: Lucia de
authors.initials: Ld
authors.lastname: Noronha
authors.forename: Antonio Marcelo Barbante
authors.initials: AM
authors.lastname: Casella
authors.forename: Thaís Isabel
authors.initials: TI
authors.lastname: Lumikoski
authors.forename: Leonardo Brandão
authors.initials: LB
authors.lastname: Précoma
authors.forename: Caroline Luzia de Almeida
authors.initials: CL
authors.lastname: Torres
authors.forename: Andréa
authors.initials: A
authors.lastname: Luchini
authors.forename: Mario Claudio Soares
authors.initials: MC
authors.lastname: Sturzeneker
authors.forename: Dalton Bertolim
authors.initials: DB
authors.lastname: Précoma
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000378:metabolism*; D002791:Cholesterol, Dietary / Q000009:adverse effects; D002829:Choroid / Q000378:metabolism*; D004195:Disease Models, Animal; D006937:Hypercholesterolemia / Q000209:etiology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D011817:Rabbits; D012590:Sclera / Q000378:metabolism*
chemical_list: D018932:Chemokine CCL2; D002791:Cholesterol, Dietary