title: Single-step turn-on homogeneous fluorescent immunosensor for rapid, sensitive, and selective detection of proteins.
abstract: Quick & easy: A simple and positive-readout fluorescent immunosensor was developed based on a quencher composed of a 3 nm gold nanoparticle (GNP) covalently linked to a Fab fragment, which proved to be stable and specific. The method allows for direct and sensitive detection of target antigens in homogeneous solutions in one step without any separation steps. It has been successfully applied to rapidly quantify the amount of secretory immunoglobulin A (sIgA) in human saliva samples.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, and College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Xiaocui
authors.initials: X
authors.lastname: Zhu
authors.forename: Dongqin
authors.initials: D
authors.lastname: Zhao
authors.forename: Yingxiao
authors.initials: Y
authors.lastname: Cai
authors.forename: Meiping
authors.initials: M
authors.lastname: Zhao
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000379:methods; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000379:methods*; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000276:immunology*; D053758:Nanoparticles / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D007071:Immunoglobulin A, Secretory; D007140:Immunoglobulin Fab Fragments; D006046:Gold; D016650:Fluorescein-5-isothiocyanate