title: Sequential elution of multiply and singly phosphorylated peptides with polar-copolymerized mixed-mode RP18/SCX material.
abstract: Novel polar-copolymerized mixed-mode RP18/SCX material was developed for feasible phosphopeptide enrichment, in which multiply and singly phosphorylated peptides could be sequentially eluted and separated with high selectivity.
authors.affiliation: Key Lab of separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, 457 Zhongshan Road, Dalian 116023, China. liangxm@dicp.ac.cn
authors.forename: Xiuling
authors.initials: X
authors.lastname: Li
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.forename: Qianying
authors.initials: Q
authors.lastname: Sheng
authors.forename: Xingya
authors.initials: X
authors.lastname: Xue
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D002364:Caseins / Q000378:metabolism; D002852:Chromatography, Ion Exchange / Q000379:methods*; D010748:Phosphopeptides / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D058105:Polymerization; D059748:Proteolysis; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013997:Time Factors
chemical_list: D002364:Caseins; D010748:Phosphopeptides; D012821:Silanes; C575101:n-octadecyl trichlorosilane-2-(4-chlorosulfonylphenyl)ethyl trichlorosilane; D012822:Silicon Dioxide