title: A semisynthetic fluorescent sensor protein for glutamate.
abstract: We report the semisynthesis of a fluorescent glutamate sensor protein on cell surfaces. Sensor excitation at 547 nm yields a glutamate-dependent emission spectrum between 550 and 700 nm that can be exploited for ratiometric sensing. On cells, the sensor displays a ratiometric change of 1.56. The high sensitivity toward glutamate concentration changes of the sensor and its exclusive extracellular localization make it an attractive tool for glutamate sensing in neurobiology.
authors.affiliation: Institute of Chemical Sciences and Engineering, National Centre of Competence in Research Chemical Biology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Matthias A
authors.initials: MA
authors.lastname: Brun
authors.forename: Kui-Thong
authors.initials: KT
authors.lastname: Tan
authors.forename: Rudolf
authors.initials: R
authors.lastname: Griss
authors.forename: Anna
authors.initials: A
authors.lastname: Kielkowska
authors.forename: Luc
authors.initials: L
authors.lastname: Reymond
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D015870:Gene Expression; D018698:Glutamic Acid / Q000032:analysis* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D017434:Protein Structure, Tertiary; D017470:Receptors, Glutamate / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D017470:Receptors, Glutamate; D011993:Recombinant Fusion Proteins; D018698:Glutamic Acid