title: Tissue processing prior to analysis of Alzheimer's disease associated proteins and metabolites, including Aβ.
abstract: Amyloid-containing tissue, whether from human patients or an animal model of a disease, is typically characterized by various biochemical and immunohistochemical techniques, many of which are described in detail in this volume. In this chapter, we describe a straightforward technique for the homogenization of tissue prior to these analyses. The technique is particularly well suited for performing a large number of different biochemical analyses on a single mouse brain hemisphere. Starting with this homogenate multiple characterizations can be done, including western blot analysis and isolation of membrane-associated proteins, both of which are described here. Additional analyses can readily be performed on the tissue homogenate, including the ELISA quantitation of Aβ in the brain of a transgenic mouse model of β-amyloid deposition. The ELISA technique is described in detail in Chapter 34.
authors.affiliation: Center for Dementia Research, Nathan S. Kline Institute for Psychiatric Research, Orangeburg, NY, USA. stephen.schmidt@nih.gov
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Schmidt
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Nixon
authors.forename: Paul M
authors.initials: PM
authors.lastname: Mathews
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism / Q000473:pathology; D004210:Dissection; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D010446:Peptide Fragments / Q000378:metabolism*; D012995:Solubility; D013395:Sucrose / Q000737:chemistry; D016707:Tissue Fixation
chemical_list: D016564:Amyloid beta-Protein Precursor; D008565:Membrane Proteins; D010446:Peptide Fragments; D013395:Sucrose