title: Micromonospora sediminicola sp. nov., isolated from marine sediment.
abstract: An actinomycete strain, designated strain SH2-13(T), was isolated from a marine sediment sample collected from the Andaman Sea of Thailand. Applying a polyphasic approach, the isolate was identified as a member of the genus Micromonospora using morphological and chemotaxonomic characteristics, including the presence of meso-diaminopimelic acid in the peptidoglycan. Whole-cell sugars were arabinose, galactose, glucose, rhamnose, ribose and xylose. Diagnostic polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and phosphoglycolipids. The major menaquinones were MK-10(H(2)), MK-10(H(4)) and MK-10(H(6)). 16S rRNA gene sequence analysis revealed similarity to Micromonospora marina JSM1-1(T) (99.1 %), Micromonospora coxensis 2-30-b(28)(T) (99.1 %), Micromonospora aurantiaca DSM 43813(T) (98.8 %) and Micromonospora chalcea DSM 43026(T) (98.7 %). However, a combination of DNA-DNA hybridization results and phenotypic properties indicated that strain SH2-13(T) ( = NBRC 107934(T) = BCC 45601(T)) should be classified as the type strain of a novel species, with the proposed name Micromonospora sediminicola sp. nov.
authors.affiliation: Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors.forename: Khomsan
authors.initials: K
authors.lastname: Supong
authors.affiliation: Biotec Culture Collection (BCC), BIOTEC Central Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.
authors.forename: Chanwit
authors.initials: C
authors.lastname: Suriyachadkun
authors.affiliation: Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Somboon
authors.initials: S
authors.lastname: Tanasupawat
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.|Center for Bioactive Natural Products from Marine Organisms and Endophytic Fungi (BNPME), Bangkok 10330, Thailand.
authors.forename: Khanit
authors.initials: K
authors.lastname: Suwanborirux
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand Science Park, Phaholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.
authors.forename: Pattama
authors.initials: P
authors.lastname: Pittayakhajonwut
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Kudo
authors.affiliation: Actinobacterial Research Unit and Microbial Resource Management Unit, Scientific Instrument Center, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.|Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors.forename: Chitti
authors.initials: C
authors.lastname: Thawai
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S