title: Microbial enhance of chitosan production by Rhizopus arrhizus using agroindustrial substrates.
abstract: This study investigated the potential of Rhizopus arrhizus UCP 402 for producing chitosan using corn steep liquor and honey as agroindustrial nitrogen and carbon sources. A complete factorial design was used to assess the improved biomass and chitosan production. The results were evaluated using Pareto charts (Statistica 7.0 software). The chitosan obtained was characterized by X-ray diffraction. The cristallinity index (I(C)), and infrared spectroscopy (FTIR) were used to evaluate the degree of deacetylation (DD %). The morphological aspects of the R. arrhizus were evaluated by measuring the diameter of the colonies by light microscopy. The results obtained showed higher biomass and chitosan yields (20.61 g/L and 29.3 mg/g), respectively, in the selected assays. The characterization of the macromolecular arrangement of chitosan showed a crystallinity index compatible with the literature, and the infrared peaks confirmed a degree of 86%. The experimental data obtained suggest that adding honey to corn steep liquor is a promising way to improve microbiological chitosan production.
authors.affiliation: Northeastern Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco, 50050-900 Recife, PE, Brazil.
authors.forename: Antonio
authors.initials: A
authors.lastname: Cardoso
authors.forename: Clarissa Izabel M
authors.initials: CI
authors.lastname: Lins
authors.forename: Ednaldo Ramos
authors.initials: ER
authors.lastname: dos Santos
authors.forename: Marta C
authors.initials: MC
authors.lastname: Freitas Silva
authors.forename: Galba M
authors.initials: GM
authors.lastname: Campos-Takaki
mesh_terms: D018533:Biomass; D002244:Carbon; D048271:Chitosan / Q000378:metabolism*; D018556:Crops, Agricultural / Q000378:metabolism*; D003460:Crystallization; D006722:Honey; D008853:Microscopy; D009584:Nitrogen; D012233:Rhizopus / Q000378:metabolism* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D002244:Carbon; D048271:Chitosan; D009584:Nitrogen