title: A general method for detecting protease activity via gelation and its application to artificial clotting.
abstract: A modular system for detecting protease activity via enzyme-triggered gel formation is described. Protease-specific recognition sequences are utilized to achieve enzyme specificity. Artificial blood clotting is demonstrated by activating endogenous thrombin to trigger gelation in fibrinogen-deficient blood plasma.
authors.affiliation: Department of Medicinal Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan, 48109-1055, USA.
authors.forename: Steven C
authors.initials: SC
authors.lastname: Bremmer
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Anne J
authors.initials: AJ
authors.lastname: McNeil
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Soellner
mesh_terms: D010129:4-Aminobenzoic Acid / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D032701:Biomimetics / Q000379:methods*; D001777:Blood Coagulation; D004151:Dipeptides / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D044367:Phase Transition; D059748:Proteolysis; D013379:Substrate Specificity; D013917:Thrombin / Q000032:analysis* / Q000737:chemistry
chemical_list: D000596:Amino Acids; D004151:Dipeptides; D005782:Gels; D013917:Thrombin; D010129:4-Aminobenzoic Acid