title: Estrogenic potential of benzotriazole on marine medaka (Oryzias melastigma).
abstract: This study, for the first time, assessed the reproductive effects of benzotriazoles, widely used industrial chemicals, on marine fish. Marine medakas (Oryzias melastigma) were exposed to 0.01, 0.1, and 1mg/L benzotriazole for periods of four and 35 days. The results that are obtained showed that the expression levels of CYP1A1 were down-regulated in the liver, gills and intestines of both males and females. Vitellogenin (VTG) was highly induced in the liver, gills and intestine of both male and female marine medaka, and CYP19a was up-regulated in the ovaries especially after being exposed for 35 days. Most importantly, the results of the present study suggest that even at environmentally relevant concentrations detected in the aquatic environment, 0.01 mg/L, benzotriazole also caused notable changes in expression levels of VTG, CYP1A1 and CYP19a. More concerns about the toxicity of benzotriazoles on marine animals should be raised.
authors.affiliation: Marine Biology Institute, Shantou University, Shantou, Guangdong 515063, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Tangtian
authors.forename: Liang
authors.initials: L
authors.lastname: Bo
authors.forename: Liu
authors.initials: L
authors.lastname: Wenhua
authors.forename: Paul K S
authors.initials: PK
authors.lastname: Shin
authors.forename: Rudolf S S
authors.initials: RS
authors.lastname: Wu
mesh_terms: D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity; D004785:Environmental Pollutants / Q000633:toxicity*; D004967:Estrogens / Q000633:toxicity*; D005260:Female; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009990:Oryzias; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects*; D014230:Triazoles / Q000633:toxicity*; D014819:Vitellogenins / Q000378:metabolism / Q000494:pharmacology
chemical_list: D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D004967:Estrogens; D014230:Triazoles; D014819:Vitellogenins; C012771:benzotriazole; D019363:Cytochrome P-450 CYP1A1