title: Invasive Escherichia coli vaccines expressing Brucella melitensis outer membrane proteins 31 or 16 or periplasmic protein BP26 confer protection in mice challenged with B. melitensis.
abstract: Because of the serious economic and medical consequences of brucellosis, efforts are to prevent infection of domestic animals through vaccines. Many disadvantages are associated with the current Brucella melitensis Rev.1 vaccine prompting development of alternative vaccines and delivery. Escherichia coli (DH5α) was engineered to express a plasmid containing the inv gene from Yersinia pseudotuberculosis and the hly gene from Listeria monocytogenes. These recombinant invasive E. coli expressing B. melitensis outer membrane proteins (Omp31 or 16) or the periplasmic protein BP26 were evaluated for protection of mice against virulent B. melitensis. Importantly, these invasive E. coli vaccines induced significant protection against B. melitensis challenged mice. Invasive E. coli may be an ideal vaccine platform with natural adjuvant properties for application against B. melitensis since the E. coli delivery system is non-pathogenic and can deliver antigens to antigen-presenting cells promoting cellular immune responses.
authors.affiliation: Central Institute for Research On Goats, Makhdoom, PO Farah, Mathura 281 122, India. gupta.drvivek@gmail.com
authors.forename: V K
authors.initials: VK
authors.lastname: Gupta
authors.forename: G
authors.initials: G
authors.lastname: Radhakrishnan
authors.forename: J
authors.initials: J
authors.lastname: Harms
authors.forename: G
authors.initials: G
authors.lastname: Splitter
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D002004:Brucella Vaccine / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D017347:Brucella melitensis / Q000235:genetics / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology / Q000517:prevention & control*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage*; D004926:Escherichia coli / Q000235:genetics*; D005260:Female; D005822:Genetic Vectors; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008089:Listeria monocytogenes / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D033903:Periplasmic Proteins / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D015011:Yersinia pseudotuberculosis / Q000235:genetics
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002004:Brucella Vaccine; D016207:Cytokines; D004337:Drug Carriers; D033903:Periplasmic Proteins