title: The majority of atypical cpb2 genes in Clostridium perfringens isolates of different domestic animal origin are expressed.
abstract: This study examined the prevalence and expression of the "consensus" and the "atypical"cpb2 genes in Clostridium perfringens isolates from cattle, chickens, dogs, goats, horses, pigs and sheep using polymerase chain reaction (PCR), sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western blotting. Almost all porcine isolates (12/14) carried and expressed the consensus form of cpb2 but, when present in 108 non-porcine isolates, the gene was usually the atypical form (40 atypical versus 9 consensus). Western blotting showed expression in 30 of 40 (75%) atypical cpb2-positive isolates, considerably more frequently than reported previously. CPB2 was expressed by almost all (20/21) the consensus cpb2-positive isolates, regardless of source.
authors.affiliation: Department of Pathobiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Jasmina
authors.initials: J
authors.lastname: Kircanski
authors.forename: Valeria R
authors.initials: VR
authors.lastname: Parreira
authors.forename: Samantha
authors.initials: S
authors.lastname: Whiteside
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Pei
authors.forename: John F
authors.initials: JF
authors.lastname: Prescott
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D015153:Blotting, Western; D003015:Clostridium Infections / Q000382:microbiology / Q000662:veterinary*; D003016:Clostridium perfringens / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D058751:Livestock / Q000382:microbiology*; D016133:Polymerase Chain Reaction
chemical_list: D001427:Bacterial Toxins; C109896:cpb2 protein, Clostridium perfringens