title: Regional and cell-type specific distribution of HDAC2 in the adult mouse brain.
abstract: The effects of epigenetics on brain functions are not completely understood, but histone deacetylases (HDACs) are known to affect brain function and dysfunction by mediating the acetylation status of target proteins, thereby affecting gene expression. The current study used immunochemistry to illuminate the regional distribution of one member of the HDAC family, HDAC2, in the C57BL/6J mouse brain. Our data show that HDAC2 is ubiquitously expressed throughout the mouse brain and is localized primarily within the cell nucleus. Using double-immunofluorescence, we demonstrated HDAC2 expression in neuronal cells, including cholinergic, serotonergic and catecholaminergic neurons, as well as postsynaptic glutamatergic and GABAergic neurons. HDAC2 was also observed in oligodendrocytes, but not in astrocytes or microglia. These detailed immunological studies illuminate the distribution of HDAC2 throughout the mouse brain and will facilitate investigation of the roles of HDAC2 in brain function and neurological disorders.
authors.affiliation: Comparative Medical Center, Peking Union Medical College (PUMC) and Institute of Laboratory Animal Science, Chinese Academy of Medical Science (CAMS), Panjiayuan Nanli NO. 5, Beijing, 100021, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Yao
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Huang
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Ma
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Sheng
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
mesh_terms: D059331:Adrenergic Neurons / Q000201:enzymology; D000367:Age Factors; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology; D059329:Cholinergic Neurons / Q000201:enzymology; D059330:GABAergic Neurons / Q000201:enzymology; D056464:Histone Deacetylase 2 / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000201:enzymology*; D009836:Oligodendroglia / Q000201:enzymology; D059326:Serotonergic Neurons / Q000201:enzymology
chemical_list: C533424:Hdac2 protein, mouse; D056464:Histone Deacetylase 2