title: Evaluation of rabbit antibody response against 8 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus.
abstract: OBJECTIVE
  To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus (E. granulosus) and measuring polyclonal antibody and humoral immune response using ELISA and gel diffusion.
  
  
  METHODS
  Two mentioned antigens were cloned and expressed in expression vector and purified by affinity chromatography. Four young rabbits were selected and challenged intradermally with yielded recombinant antigens. Rabbits' sera were collected post infection and were tested using ELISA and gel diffusion for polyclonal antibody detection 10 days after last injection.
  
  
  RESULTS
  The specific antibody against the recombinant peptides was efficiently produced within 4 weeks post infection.
  
  
  CONCLUSIONS
  Produced recombinants proteins could induce the immune response of the rabbits successfully. This process might improve the clarification of diagnosis and vaccination as regards hydatidosis.
authors.affiliation: Department of Medical Parasitology and Mycology, School of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Jahangir
authors.initials: J
authors.lastname: Abdi
authors.forename: Bahram
authors.initials: B
authors.lastname: Kazemi
authors.forename: Mohammad Hasan
authors.initials: MH
authors.lastname: Karimfar
authors.forename: Mohammad Bagher
authors.initials: MB
authors.lastname: Rokni
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000917:Antibody Formation / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology; D004443:Echinococcosis / Q000276:immunology* / Q000517:prevention & control; D048209:Echinococcus granulosus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D016052:Protozoan Vaccines; D011817:Rabbits; D011994:Recombinant Proteins
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016052:Protozoan Vaccines; D011994:Recombinant Proteins