title: Performance of electrospun poly(ε-caprolactone) fiber meshes used with mineral trioxide aggregates in a pulp capping procedure.
abstract: Living dental pulp tissue exposed to the oral environment should be protected with an appropriate pulp capping material to support the dentinogenesis potential of the pulp cells. Mineral trioxide aggregate (MTA) is the material of choice for the treatment of pulp. However, due to cytotoxicity during the initial setting phase of MTA, a new material is required that can act as a barrier to direct contact but facilitate the favorable effect of MTA. This study examined the feasibility of using electrospun poly(ε-caprolactone) fiber (PCL-F) meshes in the MTA-based pulp capping procedures. An experimental pulp capping was performed on the premolars of beagle dogs, and the efficacy of the PCL-F meshes was evaluated after 8 weeks. PCL-F/MTA formed a dentin bridge that was approximately fourfold thicker than that formed by the MTA. Columnar polarized odontoblast-like cells with long processes and tubular dentin-like matrices were observed beneath the dentin bridge in the PCL-F/MTA. The cells were also intensely immunostained for dentin sialoprotein. In cell cultures, PCL-F/MTA reduced cell death to ~8% of that in the MTA group. The proliferation of the cells cultured on PCL-F/MTA was much greater than that of cells cultured on MTA. Furthermore, PCL-F/MTA promoted the differentiation of MDPC23 cells to odontoblast-like cells and biomineralization, as confirmed by the expression of alkaline phosphatase and dentin sialophosphoprotein, and by the deposition of calcium. Based on these histologic findings and the cell responses observed in this study, PCL-F may be used efficiently in the MTA-based dental pulp therapy.
authors.affiliation: Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: WooCheol
authors.initials: W
authors.lastname: Lee
authors.forename: Joung-Hwan
authors.initials: JH
authors.lastname: Oh
authors.forename: Joo-Cheol
authors.initials: JC
authors.lastname: Park
authors.forename: Hong-In
authors.initials: HI
authors.lastname: Shin
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Baek
authors.forename: Hyun-Mo
authors.initials: HM
authors.lastname: Ryoo
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Woo
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D001641:Bicuspid / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology / Q000601:surgery; D017610:Calcium Compounds / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003782:Dental Pulp / Q000000981:diagnostic imaging / Q000187:drug effects / Q000648:ultrastructure; D003785:Dental Pulp Capping / Q000379:methods*; D003804:Dentin / Q000378:metabolism; D004285:Dogs; D004338:Drug Combinations; D008297:Male; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000494:pharmacology*; D011091:Polyesters / Q000494:pharmacology*; D011672:Pulpotomy; D017640:Silicates / Q000494:pharmacology*; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D011091:Polyesters; D017640:Silicates; C086631:mineral trioxide aggregate; C016240:polycaprolactone