title: Prevalence of five biofilm-related oral streptococci species from plaque.
abstract: OBJECTIVE
  To examine the prevalence of five oral streptococci species of severe early childhood caries (S-ECC) and caries-free (CF) groups.
  
  
  METHODS
  Supra gingival plaque samples were obtainedfrom 198 Thai children with ages ranging from one to six years old Eighty-seven subjects had no caries (dmft=0), and 111 had S-ECC. After DNA extraction, S. mutans, S. sobrinus, S. sanguinis, S. oralis, and S. gordonii were identified by standard PCR using species-specific primers. Statistical analysis determined the differences among prevalence rates of each species using Pearson chi-square test. The relationship among dmft score, age, sex and caries status within each group was analyzed by logistical regression (p < or = 0.05).
  
  
  RESULTS
  Sex was not correlated with any of the species detected in both groups (mean age =3.09, mean +/- SD of dmft = 11.04 +/- 7.89). S. mutans was found at greatest prevalence in both groups followed by S. oralis. S. gordonii was detected at a high prevalence, but S. sobrinus and S. sanguinis were lower in S-ECC when compared with those from the CF group.
  
  
  CONCLUSIONS
  S. mutans was associated significantly with S-ECC (p < or = 0.05). Caries prevalence was highest (56.5%) in subjects infected by S. mutans alone. S. sanguinis prevalence was higher in the CF group, but not statiscally different. Infection with MS did not show higher caries prevalence.
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand. mkemthong@yahoo.com
authors.forename: K
authors.initials: K
authors.lastname: Mitrakul
authors.forename: Y
authors.initials: Y
authors.lastname: Asvanund
authors.forename: K
authors.initials: K
authors.lastname: Vongsavan
mesh_terms: D018441:Biofilms; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D004245:DMF Index; D003731:Dental Caries / Q000453:epidemiology / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D006801:Humans; D015994:Incidence; D007223:Infant; D016015:Logistic Models; D008297:Male; D058889:Molecular Typing; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D013291:Streptococcus / Q000235:genetics* / Q000302:isolation & purification; D054773:Streptococcus gordonii / Q000235:genetics / Q000302:isolation & purification; D013295:Streptococcus mutans / Q000235:genetics / Q000302:isolation & purification; D019533:Streptococcus oralis / Q000235:genetics / Q000302:isolation & purification; D013785:Thailand / Q000453:epidemiology
chemical_list: D012336:RNA, Ribosomal, 16S