title: Effective T-cell recall responses require the taurine transporter Taut.
abstract: T-cell activation and the subsequent transformation of activated T cells into T-cell blasts require profound changes in cell volume. However, the impact of cell volume regulation for T-cell immunology has not been characterized. Here we studied the role of the cell-volume regulating osmolyte transporter Taut for T-cell activation in Taut-deficient mice. T-cell mediated recall responses were severely impaired in taut(-/-)  mice as shown with B16 melanoma rejection and hapten-induced contact hypersensitivity. CD4(+)  and CD8(+)  T cells were unequivocally located within peripheral lymph nodes of unprimed taut(-/-)  mice but significantly decreased in taut(-/-)  compared with taut(+/+)  mice following in vivo activation. Further analysis revealed that Taut is critical for rescuing T cells from activation-induced cell death in vitro and in vivo as shown with TCR, superantigen, and antigen-specific activation. Consequently, reduction of CD4(+)  and CD8(+)  T cells in taut(-/-)  mice upon antigen challenge resulted in impaired in vivo generation of T-cell memory. These findings disclose for the first time that volume regulation in T cells is an element in the regulation of adaptive immune responses and that the osmolyte transporter Taut is crucial for T-cell survival and T-cell mediated immune reactions.
authors.affiliation: Department of Dermatology, University of Tübingen, Tübingen, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Kaesler
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Sobiesiak
authors.forename: Manfred
authors.initials: M
authors.lastname: Kneilling
authors.forename: Thomas
authors.initials: T
authors.lastname: Volz
authors.forename: Wolfgang E
authors.initials: WE
authors.lastname: Kempf
authors.forename: Philipp A
authors.initials: PA
authors.lastname: Lang
authors.forename: Karl S
authors.initials: KS
authors.lastname: Lang
authors.forename: Thomas
authors.initials: T
authors.lastname: Wieder
authors.forename: Birgit
authors.initials: B
authors.lastname: Heller-Stilb
authors.forename: Ulrich
authors.initials: U
authors.lastname: Warskulat
authors.forename: Dieter
authors.initials: D
authors.lastname: Häussinger
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
authors.forename: Tilo
authors.initials: T
authors.lastname: Biedermann
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D007156:Immunologic Memory / Q000187:drug effects / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018089:Superantigens / Q000276:immunology / Q000494:pharmacology; D000099247:Taurine Transporters
chemical_list: D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D018089:Superantigens; D000099247:Taurine Transporters