title: Preparation of agarose/chitosan composite supermacroporous monolithic cryogels for affinity purification of glycoproteins.
abstract: Supermacroporous agarose/chitosan composite monolithic (AC CM) cryogels were prepared for affinity purification of the major egg white glycoproteins, ovalbumin (OVA), and ovotransferrin (OVT). The supermacroporous AC CM cryogels were produced by cryocopolymerization of agarose/chitosan blend solutions using glutaraldehyde as the cross-linker. The 3-aminophenlyboronic acid ligand was immobilized by covalent binding to epoxy-group-coupled supermacroporous AC CM cryogels. The microstructure morphologies of these cryogels were analyzed by scanning electron microscopy. The supermacroporous AC CM cryogels contained a continuous interpenetrating polymer network matrix with interconnected pores of 10-100 μm in size. The composite cryogels offered high mechanical stability and had specific recognition for glycoproteins. The maximum binding capacity of OVA adsorption from aqueous solutions was 55.6 mg/g. The matrix could be reused 11 times without significant loss in OVA adsorption capacity. The recovery yields of OVA and OVT from egg white were estimated to be 89 and 93%, respectively.
authors.affiliation: Faculty of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Sijuan
authors.initials: S
authors.lastname: Sun
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Tang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.forename: Li'an
authors.initials: L
authors.lastname: Guo
authors.forename: Yanding
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000327:Adsorption; D000818:Animals; D002645:Chickens; D048271:Chitosan / Q000737:chemistry; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D059985:Cryogels / Q000737:chemistry; D004527:Egg Proteins / Q000032:analysis / Q000302:isolation & purification*; D004529:Egg White / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D016062:Porosity; D012685:Sepharose / Q000737:chemistry
chemical_list: D059985:Cryogels; D004527:Egg Proteins; D006023:Glycoproteins; D012685:Sepharose; D048271:Chitosan