title: Fusobacterium nucleatum and Tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model.
abstract: Tannerella forsythia is strongly associated with chronic periodontitis, an inflammatory disease of the tooth-supporting tissues, leading to tooth loss. Fusobacterium nucleatum, an opportunistic pathogen, is thought to promote dental plaque formation by serving as a bridge bacterium between early- and late-colonizing species of the oral cavity. Previous studies have shown that F. nucleatum species synergize with T. forsythia during biofilm formation and pathogenesis. In the present study, we showed that coinfection of F. nucleatum and T. forsythia is more potent than infection with either species alone in inducing NF-κB activity and proinflammatory cytokine secretion in monocytic cells and primary murine macrophages. Moreover, in a murine model of periodontitis, mixed infection with the two species induces synergistic alveolar bone loss, characterized by bone loss which is greater than the additive alveolar bone losses induced by each species alone. Further, in comparison to the single-species infection, mixed infection caused significantly increased inflammatory cell infiltration in the gingivae and osteoclastic activity in the jaw bones. These data show that F. nucleatum subspecies and T. forsythia synergistically stimulate the host immune response and induce alveolar bone loss in a murine experimental periodontitis model.
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University at Buffalo, State University of New York, Buffalo, New York, USA.
authors.forename: Rajendra P
authors.initials: RP
authors.lastname: Settem
authors.forename: Ahmed Taher
authors.initials: AT
authors.lastname: El-Hassan
authors.forename: Kiyonobu
authors.initials: K
authors.lastname: Honma
authors.forename: Graham P
authors.initials: GP
authors.lastname: Stafford
authors.forename: Ashu
authors.initials: A
authors.lastname: Sharma
mesh_terms: D016301:Alveolar Bone Loss / Q000382:microbiology* / Q000473:pathology*; D000818:Animals; D041963:Bacteroidetes / Q000472:pathogenicity*; D060085:Coinfection / Q000382:microbiology / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D016967:Fusobacterium nucleatum / Q000472:pathogenicity*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010518:Periodontitis / Q000382:microbiology* / Q000473:pathology*