title: Isothiocyanate-drug interactions in the human adenocarcinoma cell line Caco-2.
abstract: Isothiocyanates, among which alyssin is counted, are the compounds that have proved chemopreventive properties and the ability to induce the 2 and the 3 detoxification phase by affecting the transcription factor nuclear erythroid 2-related factor (Nrf2). Having a positive effect on the human body, these compounds are used as dietary supplements. Because of the observed increase in the consumption of dietary supplements taken along with the drugs routinely used in medical practice, this study examined the possibility of interactions between alyssin and drugs, which could have an impact on cell metabolism. We have determined the effects of the tested substances and their interactions on the expression and activity of the phase 2 genes, as well as on the drug transport, which could be influenced by affecting the expression of transport proteins that belong to the 3 phase of metabolism. It was also studied whether the transcription factor Nrf2 is responsible for the interactions that occurred. The results showed that the interactions between alyssin and the tested drugs strengthen or weaken the effect of the drugs given separately depending on the concentration of alyssin and the type of drug. Even though Nrf2 is involved in the interaction, it seems that it is not the only factor regulating the interactions between the tested medications.
authors.affiliation: National Medicines Institute, Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Lubelska
authors.forename: Irena
authors.initials: I
authors.lastname: Misiewicz-Krzemińska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Milczarek
authors.forename: Jolanta
authors.initials: J
authors.lastname: Krzysztoń-Russjan
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Anuszewska
authors.forename: Karolina
authors.initials: K
authors.lastname: Modzelewska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wiktorska
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism; D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000230:Adenocarcinoma; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology; D004347:Drug Interactions; D005665:Furosemide / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D017879:Isothiocyanates / Q000494:pharmacology*; D007660:Ketoprofen / Q000494:pharmacology; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D013861:Thiocyanates / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000889:Anti-Arrhythmia Agents; D016861:Cyclooxygenase Inhibitors; D004232:Diuretics; D005665:Furosemide; D005982:Glutathione Transferase; D017879:Isothiocyanates; D007660:Ketoprofen; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D016660:NAD(P)H Dehydrogenase (Quinone); D051267:NF-E2-Related Factor 2; D013861:Thiocyanates; D014700:Verapamil; C495635:NFE2L2 protein, human; C524316:alyssin; C421109:multidrug resistance-associated protein 1