title: An association between EGF and EGFR gene polymorphisms with gastric cancer in a Chinese Han population.
abstract: OBJECTIVE
  The epidermal growth factor and its receptor play an important role in the development of gastric cancer. We investigate the potential association between epidermal growth factor and its receptor gene polymorphisms with gastric cancer in a Chinese Han population.
  
  
  METHODS
  Polymerase chain reaction restriction fragment length polymorphism strategies were used to genotype EGF +61 G/A and EGFR+2073 A/T in 207 gastric cancer cases and 318 healthy controls.
  
  
  RESULTS
  The A allele of EGF +61 G/A was less frequently in gastric cancer patients than in controls(OR=0.73, 95%CI=0.55-0.95, p=0.02). The A allele frequency was also associated with deeper tumor invasion(OR=1.74, 95%CI=1.05-2.89, p=0.03), increased lymph node metastasis (OR=1.74, 95%CI=1.08-2.82, p=0.02)and shorter survival time (95%CI=9.27-16.72, p=0.038).A T allele frequency in the EGFR +2073 A/T polymorphism was associated with increased lymph node metastasis in gastric cancer (OR=1.62, 95%CI=1.06-2.47,p=0.03).
  
  
  CONCLUSIONS
  Our results suggest that the A allele of EGF +61 G/A polymorphism decreases the risk of gastric cancer. However, the presence of either the A allele of EGF +61 G/A or the EGFR +2073 T allele indicated a poor prognosis for gastric cancer patients.
authors.affiliation: Department of Pathology, Wuhan University, Wuhan, China. guifangyang@yahoo.com.cn
authors.forename: Guifang
authors.initials: G
authors.lastname: Yang
authors.forename: Lan
authors.initials: L
authors.lastname: Rao
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Cai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D004815:Epidermal Growth Factor / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D016017:Odds Ratio; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011379:Prognosis; D018570:Risk Assessment; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D013997:Time Factors
chemical_list: D004815:Epidermal Growth Factor; C512478:EGFR protein, human; D066246:ErbB Receptors