title: Respiratory syncytial virus infection increases regulated on activation normal T cell expressed and secreted and monocyte chemotactic protein 1 levels in serum of patients with asthma and in human monocyte cultures.
abstract: BACKGROUND
  Respiratory syncytial virus (RSV) infection is associated to episodic exacerbations of asthma involving alveolar macrophages and chemokine production.
  
  
  OBJECTIVE
  The aim of this study was to determine the circulating levels of monocyte chemotactic protein 1 (MCP-1), regulated on activation normal T cell expressed and secreted (RANTES), and substance P (SP) in patients with and without asthma with acute respiratory RSV infection and the chemokine profile in RSV- infected monocyte cultures from normal individuals and individuals with asthma.
  
  
  METHODS
  In this regard, 31 adult patients with acute respiratory infection (15 patients with asthma) were studied. MCP-1, RANTES and SP were measured in serum and in supernatants from monocyte cultures by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Increased levels of MCP-1 and RANTES were observed in serum from patients with asthma related to RSV infection. RSV-infected monocyte cultures from healthy individuals showed increased content of those chemokines, and monocyte cultures from patients with asthma showed increased expression of MCP-1.
  
  
  CONCLUSIONS
  These data show that RSV infection induces increased circulating level of chemokines in patients with asthma, and this finding could be mediated in part by the interaction virus-monocyte.
authors.affiliation: Instituto de Investigaciones Clínicas Dr. Américo Negrette, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela.
authors.forename: Jenifer
authors.initials: J
authors.lastname: Gotera
authors.forename: Maria
authors.initials: M
authors.lastname: Giuffrida
authors.forename: Alibeth
authors.initials: A
authors.lastname: Mavarez
authors.forename: Hector
authors.initials: H
authors.lastname: Pons
authors.forename: John
authors.initials: J
authors.lastname: Bermudez
authors.forename: Mery
authors.initials: M
authors.lastname: Maldonado
authors.forename: Luz Marina
authors.initials: LM
authors.lastname: Espina
authors.forename: Jesus
authors.initials: J
authors.lastname: Mosquera
authors.forename: Nereida
authors.initials: N
authors.lastname: Valero
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000097:blood*; D018946:Chemokine CCL5 / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology*; D013373:Substance P / Q000097:blood; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D018932:Chemokine CCL2; D018946:Chemokine CCL5; D013373:Substance P