title: Conformational differences between two amyloid β oligomers of similar size and dissimilar toxicity.
abstract: Several protein conformational disorders (Parkinson and prion diseases) are linked to aberrant folding of proteins into prefibrillar oligomers and amyloid fibrils. Although prefibrillar oligomers are more toxic than their fibrillar counterparts, it is difficult to decouple the origin of their dissimilar toxicity because oligomers and fibrils differ both in terms of structure and size. Here we report the characterization of two oligomers of the 42-residue amyloid β (Aβ42) peptide associated with Alzheimer disease that possess similar size and dissimilar toxicity. We find that Aβ42 spontaneously forms prefibrillar oligomers at Aβ concentrations below 30 μm in the absence of agitation, whereas higher Aβ concentrations lead to rapid formation of fibrils. Interestingly, Aβ prefibrillar oligomers do not convert into fibrils under quiescent assembly conditions but instead convert into a second type of oligomer with size and morphology similar to those of Aβ prefibrillar oligomers. Strikingly, this alternative Aβ oligomer is non-toxic to mammalian cells relative to Aβ monomer. We find that two hydrophobic peptide segments within Aβ (residues 16-22 and 30-42) are more solvent-exposed in the more toxic Aβ oligomer. The less toxic oligomer is devoid of β-sheet structure, insoluble, and non-immunoreactive with oligomer- and fibril-specific antibodies. Moreover, the less toxic oligomer is incapable of disrupting lipid bilayers, in contrast to its more toxic oligomeric counterpart. Our results suggest that the ability of non-fibrillar Aβ oligomers to interact with and disrupt cellular membranes is linked to the degree of solvent exposure of their central and C-terminal hydrophobic peptide segments.
authors.affiliation: Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Ali Reza A
authors.initials: AR
authors.lastname: Ladiwala
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Litt
authors.forename: Ravi S
authors.initials: RS
authors.lastname: Kane
authors.forename: Darryl S
authors.initials: DS
authors.lastname: Aucoin
authors.forename: Steven O
authors.initials: SO
authors.lastname: Smith
authors.forename: Swarnim
authors.initials: S
authors.lastname: Ranjan
authors.forename: Judianne
authors.initials: J
authors.lastname: Davis
authors.forename: William E
authors.initials: WE
authors.lastname: Van Nostrand
authors.forename: Peter M
authors.initials: PM
authors.lastname: Tessier
mesh_terms: D000682:Amyloid; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002850:Chromatography, Gel; D002942:Circular Dichroism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D016716:PC12 Cells; D017510:Protein Folding; D017433:Protein Structure, Secondary; D051381:Rats
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides