title: Expression patterns of Ruminococcus flavefaciens 007S cellulases as revealed by zymogram approach.
abstract: The expression of Ruminococcus flavefaciens 007S cellulases in different incubation time points (growth stages) and their substrate inducibility were analyzed by comparing the zymogram expression profiles of cultures grown on insoluble cellulose (Avicel) with cellobiose-grown cultures. The molecular weights of the enzymes were compared to (putative) cellulases encoded in the R. flavefaciens FD-1 genome.
authors.affiliation: Chair for Microbiology and Microbial Biotechnology, University of Ljubljana, Groblje 3, 1230, Domžale, Slovenia.
authors.forename: Maša
authors.initials: M
authors.lastname: Vodovnik
authors.forename: Romana
authors.initials: R
authors.lastname: Marinšek Logar
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002475:Cellobiose / Q000378:metabolism; D044602:Cellulases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D057075:Enzyme Assays; D015870:Gene Expression; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017434:Protein Structure, Tertiary; D045857:Ruminococcus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D001426:Bacterial Proteins; D002475:Cellobiose; D002482:Cellulose; D044602:Cellulases