title: Resting and dobutamine stress test induced serum concentrations of brain natriuretic peptide in German Shepherd dogs.
abstract: Studies of clinical uses of brain natriuretic peptide (BNP) represent one of the most important advances in cardiology since the introduction of echocardiography as a clinical diagnostic procedure. Defining the clinical potential of BNP in canine cardiology has not been completed yet. The aim of this study is to measure BNP concentrations in healthy German Shepherd dogs of different ages as a baseline in resting and when conventional protocol of the dobutamine stress test (DST) is applied to dogs. Concentrations of BNP were measured in blood serum by the radioimmunoassay method. The values of BNP concentrations were compared to cardiac parameters obtained by standard cardiac diagnostic procedures (radiology, electrocardiography and echocardiography). No significant differences in serum BNP concentrations existed in dogs of different ages. A statistically significant increase in BNP concentrations was registered after DST. These changes in BNP concentrations were related to ST/T electrocardiographic changes, and correlated to changes in the left ventricular internal diameter in systole (LVESD). These data suggest that BNP is not increased in aged dogs with normal cardiac systolic function and renal function, and that myocardial ischemia leads to a significant increase in BNP concentrations even in dogs with normal left ventricular function.
authors.affiliation: Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, Novi Sad, Serbia. ljubicask@polj.uns.ac.rs
authors.forename: Ljubica
authors.initials: L
authors.lastname: Spasojević Kosić
authors.forename: Dragiša R
authors.initials: DR
authors.lastname: Trailović
authors.forename: Radomir
authors.initials: R
authors.lastname: Matunović
mesh_terms: D000375:Aging; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002316:Cardiotonic Agents / Q000494:pharmacology; D004280:Dobutamine / Q000494:pharmacology*; D004285:Dogs / Q000097:blood* / Q000502:physiology; D025401:Echocardiography, Stress / Q000662:veterinary*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D020097:Natriuretic Peptide, Brain / Q000097:blood / Q000378:metabolism*
chemical_list: D002316:Cardiotonic Agents; D020097:Natriuretic Peptide, Brain; D004280:Dobutamine