title: Chromatin immunoprecipitation-promoter microarray identification of genes regulated by PRDM16 in murine embryonic palate mesenchymal cells.
abstract: The transcription factor PRDM16 regulates differentiation of brown adipocyte tissue in mice. Recently, however, it has been demonstrated that genetic knockout of Prdm16 in mice leads to a complete cleft of the secondary palate in offspring. To identify genes whose promoters bind PRDM16 in mouse embryonic palate/maxillary mesenchymal cells, we have conducted a chromatin immunoprecipitation-promoter microarray analysis (ChIP-Chip). One hundred and twenty-two gene promoters were identified as capable of binding PRDM16. These could be functionally grouped to include those on genes linked to muscle development, chondrogenesis and osteogenesis, in addition to many transcription factors. These results suggest that PRDM16 may play a role in differentiation of mesenchymal cells in the embryonic secondary palate that contribute to the anterior, bony palate and posterior, muscular palate.
authors.affiliation: Department of Molecular, Cellular, and Craniofacial Biology, University of Louisville Birth Defects Center, University of Louisville, Louisville, KY 40292, USA.
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Warner
authors.forename: Partha
authors.initials: P
authors.lastname: Mukhopadhyay
authors.forename: Cindy L
authors.initials: CL
authors.lastname: Webb
authors.forename: Robert M
authors.initials: RM
authors.lastname: Greene
authors.forename: M Michele
authors.initials: MM
authors.lastname: Pisano
mesh_terms: D000818:Animals; D001665:Binding Sites; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D046228:Microarray Analysis; D010159:Palate / Q000196:embryology* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C521133:Prdm16 protein, mouse; D014157:Transcription Factors