title: [Effect of omeprazole on gene expression profile of human umbilical vein endothelial cell line and bioinformatics analysis].
abstract: OBJECTIVE
  To characterize the effect of omeprazole on the spectrum of gene expression in the cultured human umbilical vein endothelial cell (HUVEC) line (EA.hy926), and explore the underlying molecular mechanism.
  
  
  METHODS
  Affymetrix U133 plus2.0 oligonucleotide microarray was used to detect the alteration in the gene expression profiles induced by 1×10(-5) mol/L omeprazole in HUVECs. Real-time PCR was employed to verify the results of selected differentially expressed genes, and Western blotting was performed to test the expression levels of the related proteins.
  
  
  RESULTS
  A total of 282 genes were found to show at least 1.5-fold changes in EA.hy926 cells after treatment with omeprazole for 48 h, including 236 up-regulated and 46 down-regulated ones. These genes were involved in the regulation of transcription, inflammatory response, immune response, cell adherence, anti-apoptosis, and signal transduction.
  
  
  CONCLUSIONS
  Omeprazole modulates the function of endothelial cells by regulating the gene expression profiles of multiple pathways.
authors.affiliation: Department of Geriatric Hematology, General Hospital of PLA, Beijing 100853, China. liuxianfeng1970@163.com
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Liu
authors.forename: Xuechun
authors.initials: X
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Cong
authors.initials: C
authors.lastname: Ma
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
mesh_terms: D002460:Cell Line; D019295:Computational Biology; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D009853:Omeprazole / Q000494:pharmacology*; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D009853:Omeprazole