title: Complete DNA sequence analysis of enterohemorrhagic Escherichia coli plasmid pO157_2 in β-glucuronidase-positive E. coli O157:H7 reveals a novel evolutionary path.
abstract: Strains of enterohemorragic Escherichia coli (EHEC) O157:H7 that are non-sorbitol fermenting (NSF) and β-glucuronidase negative (GUD(-)) carry a large virulence plasmid, pO157 (>90,000 bp), whereas closely related sorbitol-fermenting (SF) E. coli O157:H(-) strains carry plasmid pSFO157 (>120,000 bp). GUD(+) NSF O157:H7 strains are presumed to be precursors of GUD(-) NSF O157:H7 strains that also carry pO157. In this study, we report the complete sequence of a novel virulence plasmid, pO157-2 (89,762 bp), isolated from GUD(+) NSF O157:H7 strain G5101. PCR analysis confirmed the presence of pO157-2 in six other strains of GUD(+) NSF O157:H7. pO157-2 carries genes associated with virulence (e.g., hemolysin genes) and conjugation (tra and trb genes) but lacks katP and espP present in pO157. Comparative analysis of the three EHEC plasmids shows that pO157-2 is highly related to pO157 and pSFO157 but not ancestral to pO157. These results indicated that GUD(+) NSF O157:H7 strains might not be direct precursors to GUD(-) NSF O157:H7 as previously proposed but rather have evolved independently from a common ancestor.
authors.affiliation: Department of Nutrition and Food Science, University of Maryland, College Park, Maryland, USA.
authors.forename: L V
authors.initials: LV
authors.lastname: Rump
authors.forename: J
authors.initials: J
authors.lastname: Meng
authors.forename: E A
authors.initials: EA
authors.lastname: Strain
authors.forename: G
authors.initials: G
authors.lastname: Cao
authors.forename: M W
authors.initials: MW
authors.lastname: Allard
authors.forename: N
authors.initials: N
authors.lastname: Gonzalez-Escalona
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D019453:Escherichia coli O157 / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D022621:Shiga Toxin / Q000096:biosynthesis / Q000235:genetics; D013012:Sorbitol / Q000378:metabolism; D014774:Virulence
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D013012:Sorbitol; D022621:Shiga Toxin; D005966:Glucuronidase