title: Comparison of dimethyl sulfoxide and water as solvents for echinocandin susceptibility testing by the EUCAST methodology.
abstract: Ninety-six strains of Candida, including 29 resistant and 67 susceptible isolates with mutations in the FKS1 and FKS2 genes were tested by the European Committee on Antibiotic Susceptibility Testing EDef 7.1 and 7.2 methodologies to determine the impact on the MIC when water was replaced with dimethyl sulfoxide (DMSO) as the solvent for caspofungin and micafungin. The MICs were significantly lower and the MIC ranges were narrower when DMSO was used as the solvent. The use of DMSO may help to better discriminate between susceptible and resistant populations.
authors.affiliation: Mycology Department, Spanish National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Alastruey-Izquierdo
authors.forename: Alicia
authors.initials: A
authors.lastname: Gómez-López
authors.forename: Maiken C
authors.initials: MC
authors.lastname: Arendrup
authors.forename: Cornelia
authors.initials: C
authors.lastname: Lass-Florl
authors.forename: William W
authors.initials: WW
authors.lastname: Hope
authors.forename: David S
authors.initials: DS
authors.lastname: Perlin
authors.forename: Juan L
authors.initials: JL
authors.lastname: Rodriguez-Tudela
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology; D002175:Candida / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D054714:Echinocandins / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D012997:Solvents / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D012997:Solvents; D014867:Water; D004121:Dimethyl Sulfoxide