title: Formation of one-way-structured cultured neuronal networks in microfluidic devices combining with micropatterning techniques.
abstract: We present a simple method to regulate the direction of axon development in cultured neurons using microfabrication and microfluidics techniques. We fabricate a PDMS-based device and place it onto a chemically micropatterned glass substrate. We confirm that cultured neurons extend neurites along the medium flow direction and the micropatterned regions.
authors.affiliation: Graduate School of Information Science and Technology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. yuzo_takayama@ipc.i.u-tokyo.ac.jp
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Takayama
authors.forename: Naoki
authors.initials: N
authors.lastname: Kotake
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Haga
authors.forename: Takafumi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Mabuchi
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D004622:Embryo, Mammalian; D005898:Glass; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009415:Nerve Net / Q000166:cytology / Q000196:embryology*; D016501:Neurites / Q000502:physiology; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar