title: Microdialysis: characterisation of haematomas in myocutaneous flaps by use of biochemical agents.
abstract: Metabolic markers are measured by microdialysis to detect postoperative ischaemia after reconstructive surgery with myocutaneous flaps. If a haematoma develops around the microdialysis catheter, it can result in misinterpretation of the measurements. The aim of the present study was to investigate whether a haematoma in a flap can be identified and dissociated from ischaemia, or a well-perfused flap, by a characteristic chemical profile. In 7 pigs, the pedicled rectus abdominal muscle flap was mobilised on both sides. A haematoma was made in each flap and two microdialysis catheters were placed, one in the haematoma, and the other in normal tissue. One flap was made ischaemic by ligation of the pedicle. For 6 hours, the metabolism was monitored by measurement every half-an-hour of the concentrations of glucose, lactate, pyruvate, and glycerol from all 4 catheters. After 3 hours of monitoring, intravenous glucose was given as a challenge test to identify ischaemia. The non-ischaemic flap could be differentiated from the ischaemic flap by low glucose, and high lactate, concentrations. It was possible to identify a catheter surrounded by a haematoma in ischaemic as well as non-ischaemic muscle from a low or decreasing concentration of glucose together with a low concentration of lactate. All four sites could be completely dissociated when the concentrations of glucose and lactate were evaluated and combined with the lactate:glucose ratio and a flow chart. The challenge test was useful for differentiating between haematomas in ischaemic and non-ischaemic tissue.
authors.affiliation: Institute of Clinical Medicine, Aarhus University, Aarhus, Denmark. danjakristensen@gmail.com
authors.forename: Danja Lykke
authors.initials: DL
authors.lastname: Kristensen
authors.forename: Søren A
authors.initials: SA
authors.lastname: Ladefoged
authors.forename: Erik
authors.initials: E
authors.lastname: Sloth
authors.forename: Rasmus
authors.initials: R
authors.lastname: Aagaard
authors.forename: Hanne
authors.initials: H
authors.lastname: Birke-Sørensen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002408:Catheters, Indwelling; D062905:Central Venous Catheters; D005260:Female; D005947:Glucose / Q000032:analysis; D005990:Glycerol / Q000032:analysis; D006406:Hematoma / Q000175:diagnosis* / Q000378:metabolism; D007511:Ischemia / Q000175:diagnosis / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods*; D008991:Monitoring, Physiologic / Q000379:methods; D018482:Muscle, Skeletal / Q000637:transplantation*; D019289:Pyruvic Acid / Q000032:analysis; D011897:Random Allocation; D017568:Rectus Abdominis / Q000637:transplantation; D012680:Sensitivity and Specificity; D016038:Skin Transplantation / Q000473:pathology*; D013524:Surgical Flaps / Q000098:blood supply / Q000473:pathology*; D013552:Swine; D013997:Time Factors
chemical_list: D015415:Biomarkers; D019344:Lactic Acid; D019289:Pyruvic Acid; D005947:Glucose; D005990:Glycerol