title: Histamine release-neutralization assay for sera of patients with atopic dermatitis and/or cholinergic urticaria is useful to screen type I hypersensitivity against sweat antigens.
abstract: We previously reported that about 80 % of patients with atopic dermatitis and 60 % with cholinergic urticaria revealed type I allergy against sweat, by means of skin test against autologous sweat and/or histamine-release test for peripheral blood basophils with semi-purified sweat antigen. In this study, we developed an assay for sera to neutralize histamine-releasing activity of semi-purified sweat antigen. The semi-purified sweat antigen was pre-incubated with serially diluted sera for 30 min at 37 °C and was subjected to histamine-release activity. Histamine release-neutralization (HRN) activities were calculated by measuring the amount of histamine release from basophils in the presence or absence of semi-purified sweat antigen. Of 62 subjects, 39 showed positive histamine release (≥5 %) from their basophils in response to semi-purified sweat antigen, and sera of 34 out of 39 subjects (87.2 %) were also positive in HRN activity (≥10 %). The specificity of the HRN assay was 0.522. Moreover, HRN activities in sera were largely correlated with degrees of histamine release from peripheral blood basophils of the same donors in response to sweat antigen. To identify the substance that neutralizes histamine-release activity, we removed IgE and IgG from the sera of HRN (+) subjects by column chromatography. The HRN activities in 30 out of 42 sera were largely reduced by the removal of IgG. On the other hand, sera of four subjects lost HRN activity by the removal of IgE, suggesting that the majority of HRN (+) subjects have serum IgG against the sweat antigen as well as IgE bound to peripheral basophils. Thus, the HRN assay maybe useful for the screening of type I allergy against sweat antigen.
authors.affiliation: Programs for Biomedical Research, Division of Molecular Medical Science, Department of Dermatology, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi, Minami-ku, Hiroshima, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Shindo
authors.forename: Kaori
authors.initials: K
authors.lastname: Ishii
authors.forename: Yuhki
authors.initials: Y
authors.lastname: Yanase
authors.forename: Hidenori
authors.initials: H
authors.lastname: Suzuki
authors.forename: Michihiro
authors.initials: M
authors.lastname: Hide
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000941:Antigens / Q000276:immunology*; D001491:Basophils / Q000276:immunology; D015550:Cell Degranulation / Q000276:immunology; D002648:Child; D003876:Dermatitis, Atopic / Q000150:complications / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006636:Histamine Release / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D011950:Receptors, Cholinergic / Q000378:metabolism; D013542:Sweat / Q000276:immunology*; D014581:Urticaria / Q000150:complications / Q000175:diagnosis* / Q000276:immunology; D055815:Young Adult
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D011950:Receptors, Cholinergic; D007073:Immunoglobulin E