title: [Significance of soluble interleukin-2 receptor and NK cell activity in patients with hemophagocytic lymphohistiocytosis].
abstract: This study was aimed to detect the level of soluble interleukin-2 receptor (sCD25) and cytotoxic activity of NK lymphocytes in patients with hemophagocytic lymphohistiocytosis (HLH), and to explore their clinical significance in HLH. The enzyme-linked immunosorbent assay was used to detect the sCD25 level in serum of 20 patients with HLH, 15 healthy controls, 20 cases of acute myeloid leukemia and 20 cases of systemic lupus erythematosus. The NK cell cytotoxicity in peripheral blood of patients with HLH and controls were detected by flow cytometry with CD107a antibody labeling and LDH release assay. The results indicated that the level of sCD25 in HLH patients was significantly higher than that in healthy controls and disease groups (P < 0.001). The NK cell cytotoxicity in peripheral blood detected by both methods in patients with HLH were lower than that in healthy controls (P < 0.05), and the results detected by flow cytometry correlated significantly with those by LDH release assay (r = 0.73, P < 0.05). It is concluded that detection of sCD25 levels and NK cell activity in peripheral blood in HLH is of great value. Using flow cytometry following CD107a antibody labeling to measure NK activity is a simple, stability, reproducibility method and can be used for clinical diagnosis of HLH.
authors.affiliation: Department of Hematology, Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Wang
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Hu
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Chen
authors.forename: Ji
authors.initials: J
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Wu
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Qiu
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000097:blood*; D007694:Killer Cells, Natural / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000097:blood; D051359:Lymphohistiocytosis, Hemophagocytic / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D053645:Interleukin-2 Receptor alpha Subunit