title: Membrane-perturbing properties of two Arg-rich paddle domains from voltage-gated sensors in the KvAP and HsapBK K(+) channels.
abstract: Voltage-gated K(+) channels are gated by displacement of basic residues located in the S4 helix that together with a part of the S3 helix, S3b, forms a "paddle" domain, whose position is altered by changes in the membrane potential modulating the open probability of the channel. Here, interactions between two paddle domains, KvAPp from the K(v) channel from Aeropyrum pernix and HsapBKp from the BK channel from Homo sapiens, and membrane models have been studied by spectroscopy. We show that both paddle domains induce calcein leakage in large unilamellar vesicles, and we suggest that this leakage represents a general thinning of the bilayer, making movement of the whole paddle domain plausible. The fact that HsapBKp induces more leakage than KvAPp may be explained by the presence of a Trp residue in HsapBKp. Trp residues generally promote localization to the hydrophilic-hydrophobic interface and disturb tight packing. In magnetically aligned bicelles, KvAPp increases the level of order along the whole acyl chain, while HsapBKp affects the morphology, also indicating that KvAPp adapts more to the lipid environment. Nuclear magnetic resonance (NMR) relaxation measurements for HsapBKp show that overall the sequence has anisotropic motions. The S4 helix is well-structured with restricted local motion, while the turn between S4 and S3b is more flexible and undergoes slow local motion. Our results indicate that the calcein leakage is related to the flexibility in this turn region. A possibility by which HsapBKp can undergo structural transitions is also shown by relaxation NMR, which may be important for the gating mechanism.
authors.affiliation: Department of Biochemistry and Biophysics, The Arrhenius laboratory, Stockholm University, 10691 Stockholm, Sweden.
authors.forename: Sofia
authors.initials: S
authors.lastname: Unnerståle
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Madani
authors.forename: Astrid
authors.initials: A
authors.lastname: Gräslund
authors.forename: Lena
authors.initials: L
authors.lastname: Mäler
mesh_terms: D041522:Aeropyrum / Q000737:chemistry; D000595:Amino Acid Sequence; D001120:Arginine / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D002942:Circular Dichroism; D003903:Deuterium / Q000737:chemistry; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000737:chemistry* / Q000378:metabolism*; D007930:Leucine / Q000737:chemistry; D008051:Lipid Bilayers; D008279:Magnetic Resonance Imaging; D008969:Molecular Sequence Data; D024642:Potassium Channels, Voltage-Gated / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D005452:Fluoresceins; D051036:Large-Conductance Calcium-Activated Potassium Channels; D008051:Lipid Bilayers; D024642:Potassium Channels, Voltage-Gated; D001120:Arginine; D003903:Deuterium; D007930:Leucine; C007740:fluorexon