title: Study of amyloids using yeast.
abstract: We detail some of the genetic, biochemical, and physical methods useful in studying amyloids in yeast, particularly the yeast prions. These methods include cytoduction (cytoplasmic mixing), infection of cells with prion amyloids, use of green fluorescent protein fusions with amyloid-forming proteins for cytology, protein purification and amyloid formation, and electron microscopy of filaments.
authors.affiliation: Laboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA. wickner@helix.nih.gov
authors.forename: Reed B
authors.initials: RB
authors.lastname: Wickner
authors.forename: Dmitry
authors.initials: D
authors.lastname: Kryndushkin
authors.forename: Frank
authors.initials: F
authors.lastname: Shewmaker
authors.forename: Ryan
authors.initials: R
authors.lastname: McGlinchey
authors.forename: Herman K
authors.initials: HK
authors.lastname: Edskes
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001692:Biological Transport; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K / Q000378:metabolism; D050939:Gene Fusion; D005822:Genetic Vectors / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D046529:Microscopy, Electron, Transmission; D010641:Phenotype; D011328:Prions / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014170:Transformation, Genetic; D014498:Uracil / Q000378:metabolism
chemical_list: D000682:Amyloid; D008164:Luminescent Proteins; D011328:Prions; D029701:Saccharomyces cerevisiae Proteins; D001224:Aspartic Acid; D014498:Uracil; D019286:Endopeptidase K; C006893:ureidosuccinic acid