title: MicroRNA-181b targets cAMP responsive element binding protein 1 in gastric adenocarcinomas.
abstract: MicroRNAs are a class of small endogenous non-coding RNAs that function as post-transcriptional regulators. In our previous study, we found that miR-181b was significantly downregulated in human gastric adenocarcinoma tissue samples compared to the adjacent normal gastric tissues. In this study, we confirm the down-regulation of miR-181b in human gastric cancer cell lines versus the gastric epithelial cells. Overexpression of miR-181b suppressed the proliferation and colony formation rate of gastric cancer cells. miR-181b downregulated the expression of cAMP responsive element binding protein 1 (CREB1) by binding its 3' untranslated region. Overexpression of CREB1 counteracted the suppression of growth in gastric cancer cells caused by ectopic expression of miR-181b. These results indicate that miR-181b may function as a tumor suppressor in gastric adenocarcinoma cells through negative regulation of CREB1.
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.forename: Wei-Qing
authors.initials: WQ
authors.lastname: Kong
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D020413:3' Untranslated Regions; D000230:Adenocarcinoma / Q000378:metabolism*; D049109:Cell Proliferation; D017362:Cyclic AMP Response Element-Binding Protein / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000235:genetics; D013274:Stomach Neoplasms / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D049452:Green Fluorescent Proteins