title: Glycomics using mass spectrometry.
abstract: Mass spectrometry plays an increasingly important role in structural glycomics. This review provides an overview on currently used mass spectrometric approaches such as the characterization of glycans, the analysis of glycopeptides obtained by proteolytic cleavage of proteins and the analysis of glycosphingolipids. The given examples are demonstrating the application of mass spectrometry to study glycosylation changes associated with congenital disorders of glycosylation, lysosomal storage diseases, autoimmune diseases and cancer.
authors.affiliation: Department of Parasitology, Biomolecular Mass Spectrometry Unit, Leiden University Medical Center, Albinusdreef 2, 2333ZA, Leiden, The Netherlands. M.Wuhrer@lumc.nl
authors.forename: Manfred
authors.initials: M
authors.lastname: Wuhrer
mesh_terms: D001327:Autoimmune Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018981:Congenital Disorders of Glycosylation / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015894:Genome, Human; D023281:Genomics; D006020:Glycopeptides / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D016464:Lysosomal Storage Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D013058:Mass Spectrometry / Q000379:methods*; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D006020:Glycopeptides