title: Synthesis of microwave-assisted poly(methyl vinyl ether-co-maleic anhydride)-bovine serum albumin bioconjugates.
abstract: The water-soluble poly(methyl vinyl ether-co-maleic anhydride) copolymer-bovine serum albumin bioconjugates were synthesized in the presence of 1-ethyl-3-(3-dimetilamino-propyl) carbodiimide hydrochloride as cross-linking agents via microwave-assisted and conventional methods and characterized by size-exclusion chromatography and high-performance liquid chromatography. According to size-exclusion chromatography and high-performance liquid chromatography results, the bioconjugates synthesized in the microwave-assisted method are more stable and efficient than the conventional method. The reaction time is shortened from 17 hours to 15 minutes by means of the microwave-assisted method.
authors.affiliation: Faculty of Engineering and Natural Sciences, Department of Bioengineering, Uskudar University, Uskudar, Istanbul, Turkey. mesut.karahan@gmail.com
authors.forename: Mesut
authors.initials: M
authors.lastname: Karahan
authors.forename: Sevecen
authors.initials: S
authors.lastname: Tuğlu
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Mustafaeva
mesh_terms: D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D006801:Humans; D008298:Maleates / Q000737:chemistry*; D008872:Microwaves; D014677:Pharmaceutical Vehicles / Q000737:chemistry*; D011091:Polyesters / Q000138:chemical synthesis*; D011095:Polyethylenes / Q000737:chemistry*; D011145:Polyvinyls / Q000138:chemical synthesis*; D055550:Protein Stability; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D008298:Maleates; D014677:Pharmaceutical Vehicles; D011091:Polyesters; D011095:Polyethylenes; D011145:Polyvinyls; D014614:Vaccines, Synthetic; D012710:Serum Albumin, Bovine; C043105:poly(methyl vinyl ether-co-maleic anhydride); D005022:Ethyldimethylaminopropyl Carbodiimide