title: Photothermic regulation of gene expression triggered by laser-induced carbon nanohorns.
abstract: The development of optical methods to control cellular functions is important for various biological applications. In particular, heat shock promoter-mediated gene expression systems by laser light are attractive targets for controlling cellular functions. However, previous approaches have considerable technical limitations related to their use of UV, short-wavelength visible (vis), and infrared (IR) laser light, which have poor penetration into biological tissue. Biological tissue is relatively transparent to light inside the diagnostic window at wavelengths of 650-1,100 nm. Here we present a unique optical biotechnological method using carbon nanohorn (CNH) that transforms energy from diagnostic window laser light to heat to control the expression of various genes. We report that with this method, laser irradiation within the diagnostic window resulted in effective heat generation and thus caused heat shock promoter-mediated gene expression. This study provides an important step forward in the development of light-manipulated gene expression technologies.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Osaka 563-8577, Japan. e-miyako@aist.go.jp
authors.forename: Eijiro
authors.initials: E
authors.lastname: Miyako
authors.forename: Tomonori
authors.initials: T
authors.lastname: Deguchi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Masako
authors.initials: M
authors.lastname: Yudasaka
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hagihara
authors.forename: Masanori
authors.initials: M
authors.lastname: Horie
authors.forename: Mototada
authors.initials: M
authors.lastname: Shichiri
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Yamashita
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Hashida
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shigeri
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Sumio
authors.initials: S
authors.lastname: Iijima
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods; D045744:Cell Line, Tumor; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D006358:Hot Temperature; D007834:Lasers; D008027:Light; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D041681:NIH 3T3 Cells; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000633:toxicity*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013053:Spectrophotometry
chemical_list: D037742:Nanotubes, Carbon; D049452:Green Fluorescent Proteins; D012710:Serum Albumin, Bovine