title: De novo synthesis of trans-10, cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia lipolytica.
abstract: BACKGROUND
  Conjugated linoleic acid (CLA) has many well-documented beneficial physiological effects. Due to the insufficient natural supply of CLA and low specificity of chemically produced CLA, an effective and isomer-specific production process is required for medicinal and nutritional purposes.
  
  
  RESULTS
  The linoleic acid isomerase gene from Propionibacterium acnes was expressed in Yarrowia lipolytica Polh. Codon usage optimization of the PAI and multi-copy integration significantly improved the expression level of PAI in Y. lipolytica. The percentage of trans-10, cis-12 CLA was six times higher in yeast carrying the codon-optimized gene than in yeast carrying the native gene. In combination with multi-copy integration, the production yield was raised to approximately 30-fold. The amount of trans-10, cis-12 CLA reached 5.9% of total fatty acid yield in transformed Y. lipolytica.
  
  
  CONCLUSIONS
  This is the first report of production of trans-10, cis-12 CLA by the oleaginous yeast Y. lipolytica, using glucose as the sole carbon source through expression of linoleic acid isomerase from Propionibacterium acnes.
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi 214122, People's Republic of China.
authors.forename: Baixi
authors.initials: B
authors.lastname: Zhang
authors.forename: Chunchi
authors.initials: C
authors.lastname: Rong
authors.forename: Haiqin
authors.initials: H
authors.lastname: Chen
authors.forename: Yuanda
authors.initials: Y
authors.lastname: Song
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007535:Isomerases / Q000235:genetics / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis*; D011425:Propionibacterium acnes / Q000201:enzymology; D013237:Stereoisomerism; D025062:Yarrowia / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D044243:Linoleic Acids, Conjugated; C496197:trans-10,cis-12-conjugated linoleic acid; D007535:Isomerases; D005947:Glucose