title: Membrane-based continuous remover of trifluoroacetic acid in mobile phase for LC-ESI-MS analysis of small molecules and proteins.
abstract: We developed a "continuous" trifluoroacetic acid (TFA) remover based on electrodialysis with bipolar membrane for online coupling of liquid chromatography (LC) and electrospray ionization mass spectrometry (ESI-MS) using TFA containing mobile phase. With the TFA remover as an interface, the TFA anion in the mobile phase was removed based on electrodialysis mechanism, and meanwhile, the anion exchange membrane was self-regenerated by the hydroxide ions produced by the bipolar membrane. So the remover could continuously work without any additional regeneration process. The established LC-TFA remover-MS system has been successfully applied for the qualitative and quantitative analysis of small molecules as well as proteins.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
authors.forename: Zhigui
authors.initials: Z
authors.lastname: Zhou
authors.forename: Jialing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jiawei
authors.initials: J
authors.lastname: Xing
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.forename: Yiping
authors.initials: Y
authors.lastname: Liao
authors.forename: Huwei
authors.initials: H
authors.lastname: Liu
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D003956:Dialysis; D006878:Hydroxides / Q000737:chemistry; D007474:Ion Exchange; D008567:Membranes, Artificial; D011506:Proteins / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014269:Trifluoroacetic Acid / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D006878:Hydroxides; D008567:Membranes, Artificial; D011506:Proteins; C031356:hydroxide ion; D014269:Trifluoroacetic Acid