title: The interplay between thiol-compounds against chromium (VI) in the freshwater green alga Monoraphidium convolutum: toxicology, photosynthesis, and oxidative stress at a glance.
abstract: In this paper, the multifaceted Cr(VI) toxicity over the freshwater green alga Monoraphidium convolutum was assessed by concomitantly monitoring thiol-dependent redox balances, photosynthesis activity and growth-survival scores. Control group showed exponential growth rate at (5.78±0.29) division/day until 8th day with linear increasing chlorophyll a/protein ratios (CHLa/PROT) throughout the period. Cultures of M. convolutum were exposed for 5 days to Cr(VI) concentrations from 0 up to 100mg/L showing that CHLa/PROT ratios were sensibly affected, in agreement to the calculated LC(50,48 h) (5.38±0.72) mg/L from the concentration-response curve of cell mortality after 48 h. Regarding photosynthesis effects, Cr(VI) concentrations >1.0 mg/L showed significant increases in short-term (after 2 h) electron transfer rates (ETR) and quantum yields of photosystem II (Φ(PSII)), followed by subsequent decline of both parameters after 48 and 72 h. Biochemical analyses showed that maximal GSH concentrations in algal cultures were observed upon 1mg Cr(VI)/L and higher dichromate concentrations dramatically increased the activity of antioxidant GSH-dependent enzymes ascorbate peroxidase and glutathione reductase. However, no variation was observed in the cellular GSH levels, whereas GSSG and lipid peroxidation indexes abruptly increased upon 10 mg Cr(VI)/L exposure. Altogether, plant physiology, photosynthesis and biochemical data suggest that the GSH-dependent antioxidant system is capable to sustain M. convolutum viability through efficient photosynthesis activity and adequate antioxidant responses up to Cr(VI) concentrations of 1.0mg/L, when redox unbalances were first evidenced.
authors.affiliation: Postgraduate Program in Environmental Chemistry, CBS, Universidade Cruzeiro do Sul, 08060070, São Paulo, SP, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Takami
authors.affiliation: Department of Biochemistry, Instituto de Química, Universidade de São Paulo (IQ-USP), São Paulo, SP, Brazil.
authors.forename: J V
authors.initials: JV
authors.lastname: Almeida
authors.affiliation: Postgraduate Program in Environmental Chemistry, CBS, Universidade Cruzeiro do Sul, 08060070, São Paulo, SP, Brazil.
authors.forename: C V
authors.initials: CV
authors.lastname: Vardaris
authors.affiliation: Department of Biochemistry, Instituto de Química, Universidade de São Paulo (IQ-USP), São Paulo, SP, Brazil.
authors.forename: P
authors.initials: P
authors.lastname: Colepicolo
authors.affiliation: Postgraduate Program in Environmental Chemistry, CBS, Universidade Cruzeiro do Sul, 08060070, São Paulo, SP, Brazil. Electronic address: marcelo.barros@cruzeirodosul.edu.br.
authors.forename: M P
authors.initials: MP
authors.lastname: Barros
mesh_terms: D060387:Ascorbate Peroxidases / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D000460:Chlorophyta / Q000187:drug effects* / Q000378:metabolism; D002857:Chromium / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010788:Photosynthesis / Q000187:drug effects; D045332:Photosystem II Protein Complex / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D045332:Photosystem II Protein Complex; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D002857:Chromium; D002734:Chlorophyll; C074702:chromium hexavalent ion; D060387:Ascorbate Peroxidases; D005980:Glutathione Reductase; D005978:Glutathione; D000077194:Chlorophyll A