title: Principles for derivation of human embryonic stem cells.
abstract: This chapter describes the principles for derivation and maintenance of human embryonic stem cells. Detailed protocols are outlined and researchers who are generally skilled in mammalian cell culture should be able to repeat the processes successfully. Further, the protocols are intended for scientists who do not have access to advanced IVF equipment and therefore cannot perform, e.g. assisted hatching. In addition to derivation, we also discuss characterisation and banking of hES cells.
authors.affiliation: Cellartis AB, Dundee, UK. mikael.englund@cellartis.com
authors.forename: Mikael C O
authors.initials: MC
authors.lastname: Englund
authors.forename: Catharina
authors.initials: C
authors.lastname: Ellerström
authors.forename: Katarina
authors.initials: K
authors.lastname: Andersson
authors.forename: Karin
authors.initials: K
authors.lastname: Noaksson
authors.forename: Johan
authors.initials: J
authors.lastname: Hyllner
mesh_terms: D001755:Blastocyst / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans