title: RNA-seq analysis reveals that an ECF σ factor, AcsS, regulates achromobactin biosynthesis in Pseudomonas syringae pv. syringae B728a.
abstract: Iron is an essential micronutrient for Pseudomonas syringae pv. syringae strain B728a and many other microorganisms; therefore, B728a has evolved methods of iron acquirement including the use of iron-chelating siderophores. In this study an extracytoplasmic function (ECF) sigma factor, AcsS, encoded within the achromobactin gene cluster is shown to be a major regulator of genes involved in the biosynthesis and secretion of this siderophore. However, production of achromobactin was not completely abrogated in the deletion mutant, implying that other regulators may be involved such as PvdS, the sigma factor that regulates pyoverdine biosynthesis. RNA-seq analysis identified 287 genes that are differentially expressed between the AcsS deletion mutant and the wild type strain. These genes are involved in iron response, secretion, extracellular polysaccharide production, and cell motility. Thus, the transcriptome analysis supports a role for AcsS in the regulation of achromobactin production and the potential activity of both AcsS and achromobactin in the plant-associated lifestyle of strain B728a.
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Jessica W
authors.initials: JW
authors.lastname: Greenwald
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Greenwald
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Philmus
authors.forename: Tadhg P
authors.initials: TP
authors.lastname: Begley
authors.forename: Dennis C
authors.initials: DC
authors.lastname: Gross
mesh_terms: D001427:Bacterial Toxins / Q000378:metabolism; D001692:Biological Transport; D002951:Citrates / Q000096:biosynthesis* / Q000378:metabolism; D004777:Environment; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D015723:Gene Library; D023061:Gene Order; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D007501:Iron / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D005810:Multigene Family; D010935:Plant Diseases / Q000209:etiology / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D044224:Pseudomonas syringae / Q000235:genetics* / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D001427:Bacterial Toxins; D002951:Citrates; D007656:Ketoglutaric Acids; D011135:Polysaccharides, Bacterial; D012808:Sigma Factor; C413577:achromobactin; C064841:exopolysaccharide, Pseudomonas; D007501:Iron