title: Effect of the o-methyl catechols apocynin, curcumin and vanillin on the cytotoxicity activity of tamoxifen.
abstract: Apocynin (APO), curcumin (CUR) and vanillin (VAN) are o-methyl catechols widely studied due their antioxidant and antitumour properties. The effect of treatment with these o-methyl catechols on tamoxifen (TAM)-induced cytotoxicity in normal and tumour cells was studied. The cytotoxicity of TAM on red blood cells (RBC) was performed by haemoglobin or K(+)release and on polymorphonuclear leukocytes (PMNs) by trypan blue dye exclusion method. Cytotoxic activity was assessed in human chronic myeloid leukemia (K562) cell line by (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide). According the release of haemoglobin and K(+), the CUR showed a decrease in TAM cytotoxicity on RBC; however, in PMN, APO, CUR and VAN showed increased of these cells viability. VAN presented the highest cytotoxicity on K562 cells, followed by APO and CUR. These results point the potential therapeutic value of these o-methyl catechols with TAM, particularly of CUR, which potentiates the cytotoxic effects of TAM on K562 cells and also decreases TAM-associated cytotoxicity on RBC and PMN.
authors.affiliation: Departamento de Farmácia, Universidade Estadual do Centro-Oeste, Guarapuava, Paraná, Brasil.
authors.forename: Lígia Santos
authors.initials: LS
authors.lastname: Pedroso
authors.forename: Giovani Marino
authors.initials: GM
authors.lastname: Fávero
authors.forename: Luciana Erzinger Alves
authors.initials: LE
authors.lastname: de Camargo
authors.forename: Rubiana Mara
authors.initials: RM
authors.lastname: Mainardes
authors.forename: Najeh Maissar
authors.initials: NM
authors.lastname: Khalil
mesh_terms: D000098:Acetophenones / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001547:Benzaldehydes / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004912:Erythrocytes / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells; D015394:Molecular Structure; D009504:Neutrophils / Q000187:drug effects*; D013329:Structure-Activity Relationship; D013629:Tamoxifen / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000098:Acetophenones; D000970:Antineoplastic Agents; D001547:Benzaldehydes; D013629:Tamoxifen; C056165:acetovanillone; C100058:vanillin; D003474:Curcumin