title: 4-(2-Hydroxypropan-2-yl)-1-methylcyclohexane-1,2-diol prevents xenobiotic induced cytotoxicity.
abstract: Currently there is a great deal of interest in the study of natural compounds with free radical scavenging activity because of their potential role in maintaining human health and preventing diseases. In this paper, we report the antioxidant and cytoprotective properties of 4-(2-hydroxypropan-2-yl)-1-methylcyclohexane-1,2-diol (HPMCD) isolated from the aqueous extract of Decalepis hamiltonii roots. Our results show that HPMCD is a potent scavenger of superoxide (O(2)(*-)), hydroxyl ((*)OH), nitric oxide ((*)NO), and lipid peroxide (LOO(*)) physiologically relevant free radicals with IC(50) values in nmolar (56-582) range. HPMCD also exhibited concentration dependent secondary antioxidant activities like reducing power, metal chelating activity, and inhibition of protein carbonylation. Further, HPMCD at nmolar concentration prevented CuSO(4)-induced human LDL oxidation. Apart from the in vitro free radical scavenging activity HPMCD demonstrated cytoprotective activity in primary hepatocytes and ehrlich ascites tumor (EAT) cells against oxidative stress inducing xenobiotics. The mechanism of cytoprotective action involved maintaining the intracellular glutathione (GSH), scavenging of reactive oxygen species (ROS), and inhibition of lipid peroxidation (LPO). Based on the results it is suggested that HPMCD is a novel bioactive molecule with health implications in both prevention and amelioration of diseases involving oxidative stress as well as in the general well being.
authors.affiliation: Department of Food Protectants and Infestation Control, Spices and Flavor Technology, Central Food Technological Research Institute, Mysore 570020, Karnataka, India. anup@uab.edu
authors.forename: Anup
authors.initials: A
authors.lastname: Srivastava
authors.forename: L Jagan Mohan
authors.initials: LJ
authors.lastname: Rao
authors.forename: T
authors.initials: T
authors.lastname: Shivanandappa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D029062:Apocynaceae; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D008078:Cholesterol, LDL / Q000378:metabolism; D003511:Cyclohexanols / Q000494:pharmacology*; D019610:Cytoprotection / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D018517:Plant Roots; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: C577055:4-(2-hydroxypropan-2-yl)-1-methylcyclohexane-1,2-diol; D000975:Antioxidants; D008078:Cholesterol, LDL; D003511:Cyclohexanols; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D015262:Xenobiotics; D007770:L-Lactate Dehydrogenase; D005978:Glutathione