title: Absence of association between ex vivo susceptibility to doxycycline and pftetQ and pfmdt copy numbers in Plasmodium falciparum isolates from Dakar, Senegal.
abstract: The objective of this study was to validate the use of pftetQ and pfmdt genes as molecular markers of decreased in vitro susceptibility to doxycycline in 113 Plasmodium falciparum isolates from Dakar, Senegal. The results show that copy numbers of pftetQ and pfmdt, estimated by TaqMan real-time PCR, are not significantly associated with reduced susceptibility to doxycycline in vitro; however, the number of samples with a high doxycycline IC(50) was likely to be too low to derive statistically significant results. Thus, no definitive conclusions could be drawn. The markers should be further tested by analysing more isolates.
authors.affiliation: Unité de Parasitologie - Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes - UMR 6236, Institut de Recherche Biomédicale des Armées, Marseille, France.
authors.forename: T
authors.initials: T
authors.lastname: Gaillard
authors.forename: B
authors.initials: B
authors.lastname: Fall
authors.forename: A
authors.initials: A
authors.lastname: Tall
authors.forename: N
authors.initials: N
authors.lastname: Wurtz
authors.forename: B
authors.initials: B
authors.lastname: Diatta
authors.forename: M
authors.initials: M
authors.lastname: Lavina
authors.forename: K B
authors.initials: KB
authors.lastname: Fall
authors.forename: F D
authors.initials: FD
authors.lastname: Sarr
authors.forename: E
authors.initials: E
authors.lastname: Baret
authors.forename: Y
authors.initials: Y
authors.lastname: Diémé
authors.forename: B
authors.initials: B
authors.lastname: Wade
authors.forename: R
authors.initials: R
authors.lastname: Bercion
authors.forename: S
authors.initials: S
authors.lastname: Briolant
authors.forename: B
authors.initials: B
authors.lastname: Pradines
mesh_terms: D000962:Antimalarials / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000235:genetics*; D004318:Doxycycline / Q000494:pharmacology*; D004351:Drug Resistance; D018628:Gene Dosage; D005838:Genotype; D006801:Humans; D020128:Inhibitory Concentration 50; D021261:Parasitic Sensitivity Tests / Q000379:methods; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012675:Senegal
chemical_list: D000962:Antimalarials; D016054:DNA, Protozoan; D004318:Doxycycline