title: Ultrasensitive determination of jasmonic acid in plant tissues using high-performance liquid chromatography with fluorescence detection.
abstract: An ultrasensitive and selective high-performance liquid chromatographic method for the volatile signaling hormone, jasmonic acid, has been developed based on precolumn derivatization with 1,3,5,7-tetramethyl-8-aminozide-difluoroboradiaza-s-indacene (BODIPY-aminozide). The derivatization reaction was carried out at 60 °C for 30 min in the presence of phosphoric acid. The formed jasmonic acid derivative was eluted using a mobile phase of methanol/pH 6.50 ammonium formate buffer/tetrahydrofuran (67:30:3, v/v/v) in 10 min on a C(18) column and detected with fluorescence detection at excitation and emission wavelengths of 495 and 505 nm, respectively. The detection limit (signal-to-noise ratio = 4) reached 1.14 × 10(-10) M or 2.29 fmol per injection (20 μL), which is the lowest of the existing methods. The proposed method has been successfully applied to the direct determination of trace jasmonic acid in the crude extracts of soybean leaves from soybean mosaic virus-infected and normal plants with recoveries of 95-104%.
authors.affiliation: Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization,  Huanggang Normal University , Huangzhou 438000, China.
authors.forename: Xu-Jie
authors.initials: XJ
authors.lastname: Xiong
authors.forename: Wan-Bing
authors.initials: WB
authors.lastname: Rao
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Guo
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Zhang
mesh_terms: D001896:Boron Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003517:Cyclopentanes / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes; D009029:Mosaic Viruses; D054883:Oxylipins / Q000032:analysis*; D010935:Plant Diseases / Q000821:virology; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000737:chemistry* / Q000821:virology
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D003517:Cyclopentanes; D005456:Fluorescent Dyes; D054883:Oxylipins; D010936:Plant Extracts; C011006:jasmonic acid