title: Cyclic AMP-receptor protein activates aerobactin receptor IutA expression in Vibrio vulnificus.
abstract: The ferrophilic bacterium Vibrio vulnificus can utilize the siderophore aerobactin of Escherichia coli for iron acquisition via its specific receptor IutA. This siderophore piracy by V. vulnificus may contribute to its survival and proliferation, especially in mixed bacterial environments. In this study, we examined the effects of glucose, cyclic AMP (cAMP), and cAMP-receptor protein (Crp) on iutA expression in V. vulnificus. Glucose dose-dependently repressed iutA expression. A mutation in cya encoding adenylate cyclase required for cAMP synthesis severely repressed iutA expression, and this change was recovered by in trans complementing cya or the addition of exogenous cAMP. Furthermore, a mutation in crp encoding Crp severely repressed iutA expression, and this change was recovered by complementing crp. Accordingly, glucose deprivation under iron-limited conditions is an environmental signal for iutA expression, and Crp functions as an activator that regulates iutA expression in response to glucose availability.
authors.affiliation: Research Center for Resistant Cells, Chosun University Medical School, Gwangju, 501-759, Republic of Korea.
authors.forename: Choon-Mee
authors.initials: CM
authors.lastname: Kim
authors.forename: Seong-Jung
authors.initials: SJ
authors.lastname: Kim
authors.forename: Sung-Heui
authors.initials: SH
authors.lastname: Shin
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002373:Cyclic AMP Receptor Protein / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D041261:Vibrio vulnificus / Q000235:genetics / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein; C034506:aerobactin receptor; D007501:Iron; D005947:Glucose