title: Surface modifications by gas plasma control osteogenic differentiation of MC3T3-E1 cells.
abstract: Numerous studies have shown that the physicochemical properties of biomaterials can control cell activity. Cell adhesion, proliferation, differentiation as well as tissue formation in vivo can be tuned by properties such as the porosity, surface micro- and nanoscale topography and chemical composition of biomaterials. This concept is very appealing for tissue engineering since instructive properties in bioactive materials can be more economical and time efficient than traditional strategies of cell pre-differentiation in vitro prior to implantation. The biomaterial surface, which is easy to modify due to its accessibility, may provide the necessary signals to elicit a certain cellular behavior. Here, we used gas plasma technology at atmospheric pressure to modify the physicochemical properties of polylactic acid and analyzed how this influenced pre-osteoblast proliferation and differentiation. Tetramethylsilane and 3-aminopropyl-trimethoxysilane with helium as a carrier gas or a mixture of nitrogen and hydrogen were discharged to polylactic acid discs to create different surface chemical compositions, hydrophobicity and microscale topographies. Such modifications influenced protein adsorption and pre-osteoblast cell adhesion, proliferation and osteogenic differentiation. Furthermore polylactic acid treated with tetramethylsilane enhanced osteogenic differentiation compared to the other surfaces. This promising surface modification could be further explored for potential development of bone graft substitutes.
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Research and Technical Medicine, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: Ana M C
authors.initials: AM
authors.lastname: Barradas
authors.forename: Kristina
authors.initials: K
authors.lastname: Lachmann
authors.forename: Gregor
authors.initials: G
authors.lastname: Hlawacek
authors.forename: Cathelijne
authors.initials: C
authors.lastname: Frielink
authors.forename: Roman
authors.initials: R
authors.lastname: Truckenmoller
authors.forename: Otto C
authors.initials: OC
authors.lastname: Boerman
authors.forename: Raoul
authors.initials: R
authors.lastname: van Gastel
authors.forename: Henk
authors.initials: H
authors.lastname: Garritsen
authors.forename: Michael
authors.initials: M
authors.lastname: Thomas
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Moroni
authors.forename: Clemens
authors.initials: C
authors.lastname: van Blitterswijk
authors.forename: Jan
authors.initials: J
authors.lastname: de Boer
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D001274:Atmospheric Pressure; D001672:Biocompatible Materials / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D019344:Lactic Acid / Q000494:pharmacology; D051379:Mice; D018625:Microscopy, Atomic Force; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D058626:Plasma Gases / Q000494:pharmacology*; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013499:Surface Properties / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D058626:Plasma Gases; D011091:Polyesters; D011108:Polymers; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; C033616:poly(lactide)