title: Cell-to-cell transmission of α-synuclein aggregates.
abstract: It is now recognized that the cell-to-cell transmission of misfolded proteins such as α-synuclein contributes to the neurodegenerative phenotype in neurological disorders such as idiopathic Parkinson's disease, Dementia with Lewy bodies, and Parkinson's disease dementia. Thus, establishing cell-based models for the transmission of α-synuclein is of importance to understand the mechanisms of neurodegeneration in these disorders and to develop new therapies. Here we describe methods to study the neuron-to-neuron propagation of α-synuclein in an in vitro setting that also has in vivo applications.
authors.affiliation: Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Korea.
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Paula
authors.initials: P
authors.lastname: Desplats
authors.forename: He-Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Brian
authors.initials: B
authors.lastname: Spencer
authors.forename: Eliezer
authors.initials: E
authors.lastname: Masliah
mesh_terms: D000818:Animals; D002450:Cell Communication; D049109:Cell Proliferation; D002498:Centrifugation; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology; D017510:Protein Folding; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D021381:Protein Transport; D013194:Staining and Labeling; D014162:Transfection; D051844:alpha-Synuclein / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D051844:alpha-Synuclein