title: Physically crosslinked gelatins functionalized with tyrosine moieties do not induce angiogenesis or thrombus formation in the developing vasculature in the avian chorioallantoic membrane.
abstract: Gelatins functionalized with desaminotyrosine or desaminotyrosyl tyrosine form physically crosslinked polymer networks due to the interactions between the introduced aromatic moeties. In the swollen state, their mechanical properties can be tailored in a range similar to the elasticity of soft tissues. The aim of this study was to evaluate their potential as biomaterials by determining whether these materials - in comparison to plain gelatin - induce bleedings, thrombotic processes, or angiogenesis. These investigations were performed using the hen's egg chorioallantoic membrane (HETCAM) assay. These results indicate that the gelatin-based hydrogels did not possess angiogenic effects and also did not induce bleedings, thrombotic processes or vessel destruction (avascular zones). The biocompatibility of the materials in vitro motivates the exploration of their application as matrix in local drug-release systems with short half-life times (1 hour up to several days).
authors.affiliation: Centre for Biomaterial Development and Berlin-Brandenburg, Centre for Regenerative Therapies, Institute of Polymer Research, Helmholtz-Zentrum Geesthacht, Teltow, Germany. friedrich.jung@hzg.de
authors.forename: F
authors.initials: F
authors.lastname: Jung
authors.forename: J
authors.initials: J
authors.lastname: Goers
authors.forename: T
authors.initials: T
authors.lastname: Roch
authors.forename: A
authors.initials: A
authors.lastname: Zaupa
authors.forename: B F
authors.initials: BF
authors.lastname: Pierce
authors.forename: A T
authors.initials: AT
authors.lastname: Neffe
authors.forename: A
authors.initials: A
authors.lastname: Lendlein
mesh_terms: D000818:Animals; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply* / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D014443:Tyrosine; D005780:Gelatin