title: Thawing of cryopreserved hematopoietic progenitor cells from apheresis with a new dry-warming device.
abstract: BACKGROUND
  Current thawing techniques of cryopreserved progenitor cells are based on the use of a water bath. The aim of this study has been to assess the progenitor cell viability and the time of hematopoietic engraftment after transplantation of cell products thawed with a new dry-thawing device.
  
  
  METHODS
  In the preclinical phase, two cryobags from the same patient were thawed with the standard technique and with the dry system method in parallel (n=5, Protocol A and Protocol B, respectively). In the clinical phase, cryobags were thawed with the dry system and the time to hematopoietic engraftment after autologous transplantation (n=52) was compared with those of a control group of patients whose progenitor cell products were thawed with the standard technique (n=52).
  
  
  RESULTS
  There were no statistical differences in nuclear and CD34+ cell viability, total colony-forming cells, and cloning efficiency after thawing with Protocols A and B. Days to neutrophil (>0.5×10(9) and >1×10(9) /L) and platelet engraftment (>20×10(9) and >50×10(9) /L) were not different between patients transplanted with products thawed with Protocols A and B.
  
  
  CONCLUSIONS
  Progenitor cell viability and function are preserved with this dry-thawing system. The time to hematopoietic engraftment of patients after transplantation is comparable to those infused with progenitor cells thawed with the water bath technique. Thawing cell products without the use of water and in a dry environment might favor the use of this dry method.
authors.affiliation: Blood and Tissue Bank, Blood Division; and the Hospital Duran i Reynals, L'Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: Elvira
authors.initials: E
authors.lastname: Triana
authors.forename: Sandra
authors.initials: S
authors.lastname: Ortega
authors.forename: Carmen
authors.initials: C
authors.lastname: Azqueta
authors.forename: Helena
authors.initials: H
authors.lastname: Pomares
authors.forename: Elena
authors.initials: E
authors.lastname: Valdivia
authors.forename: Rafael
authors.initials: R
authors.lastname: Duarte
authors.forename: Lluis
authors.initials: L
authors.lastname: Massuet
authors.forename: Gregorio Angel
authors.initials: GA
authors.lastname: Martín-Henao
mesh_terms: D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation / Q000295:instrumentation* / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D018952:Antigens, CD34