title: Persistent disruption of ciliated epithelium following paediatric lung transplantation.
abstract: It is unclear whether ciliary function following lung transplantation is normal or not. Our aim was to study the ciliary function and ultrastructure of epithelium above and below the airway anastomosis and the peripheral airway of children following lung transplantation. We studied the ciliary beat frequency (CBF) and beat pattern, using high speed digital video imaging and ultrastructure by transmission electron microscopy, of bronchial epithelium from above and below the airway anastomosis and the peripheral airway of 10 cystic fibrosis (CF) and 10 non-suppurative lung disease (NSLD) paediatric lung transplant recipients. Compared to epithelium below the anastomosis, the epithelium above the anastomosis in the CF group showed reduced CBF (median (interquartile range): 10.5 (9.0-11.4) Hz versus 7.4 (6.4-9.2) Hz; p<0.01) and increased dyskinesia (median (IQR): 16.5 (12.9-28.2)% versus 42.2 (32.6-56.4)%; p<0.01). In both CF and NSLD groups, compared with epithelium above the anastomosis, the epithelium below the anastomosis showed marked ultrastructural abnormalities (median duration post-transplant 7-12 months). Ciliary dysfunction is a feature of native airway epithelium in paediatric CF lung transplant recipients. The epithelium below the airway anastomosis shows profound ultrastructural abnormalities in both CF and NSLD lung transplant recipients, many months after transplantation.
authors.affiliation: Immunity and Inflammation, University of Leicester, Leicester, UK.
authors.forename: Biju
authors.initials: B
authors.lastname: Thomas
authors.forename: Paul
authors.initials: P
authors.lastname: Aurora
authors.forename: Helen
authors.initials: H
authors.lastname: Spencer
authors.forename: Martin
authors.initials: M
authors.lastname: Elliott
authors.forename: Andrew
authors.initials: A
authors.lastname: Rutman
authors.forename: Robert A
authors.initials: RA
authors.lastname: Hirst
authors.forename: Christopher
authors.initials: C
authors.lastname: O'Callaghan
mesh_terms: D000293:Adolescent; D000714:Anastomosis, Surgical; D001980:Bronchi / Q000503:physiopathology* / Q000601:surgery / Q000648:ultrastructure*; D002648:Child; D002923:Cilia / Q000502:physiology / Q000648:ultrastructure; D004848:Epithelium / Q000503:physiopathology / Q000648:ultrastructure; D005260:Female; D006801:Humans; D016040:Lung Transplantation; D008297:Male