title: Dual roles of hyaluronic acids in multilayer films capturing nanocarriers for drug-eluting coatings.
abstract: We developed hyaluronic acid (HA)-based multilayer films capturing polymeric nanocarriers (NCs) for drug delivery. The electrostatic interactions between positively charged linear polyethylene imines (LPEI) and negatively charged HAs are the main driving forces to form multilayers based on the layer-by-layer (LbL) deposition. NCs were easily incorporated within the multilayer film due to intra- and/or inter-hydrogen bonding among HA chains. The amount of NCs captured by the HA chains was varied by the ratio between HAs and NCs as well as the length (i.e., molecular weight) and absolute number density of HAs in solution. Biocompatibility of the NC-capturing HA multilayer films was tested with the human dermal fibroblast (HDF) culture. In addition, the controlled release of paclitaxel (PTX) from the HA multilayer films successfully led to the apoptosis of human aortic smooth muscle cells (hSMC) in vitro, implying that the NC-capturing HA multilayer films would be quite useful as drug-eluting stent systems to prevent the restenosis after surgery.
authors.affiliation: The National Creative Research Initiative Center for Intelligent Hybrids, The WCU Program of Chemical Convergence of Energy & Environment, Seoul National University, Seoul 151-744, South Korea.
authors.forename: Saibom
authors.initials: S
authors.lastname: Park
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Bhang
authors.forename: Wan-Geun
authors.initials: WG
authors.lastname: La
authors.forename: Jinhwa
authors.initials: J
authors.lastname: Seo
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Kookheon
authors.initials: K
authors.lastname: Char
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D020405:Dermis / Q000166:cytology; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D054855:Drug-Eluting Stents; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D017239:Paclitaxel / Q000494:pharmacology; D010316:Particle Size
chemical_list: D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D006820:Hyaluronic Acid; D017239:Paclitaxel