title: Polydiacetylene single-walled carbon nanotubes nano-hybrid for cellular imaging applications.
abstract: The functionalization of single-walled carbon nanotubes (SWCNTs) by forming self-assembled supramolecular structure of 10,12-pentacosadiynoic acid (PCDA) on the carbon nanotube wall is reported. PCDA assemblies on SWCNTs (PCDA/SWCNTs) were polymerized by UV irradiation to extensively conjugated polydiacetylene (PDA). PDA/SWCNT was identified by absorption and emission spectroscopy, scanning and transmission electron microscopies (SEM and TEM) and atomic force microscopy (AFM). PDA/SCWNTs showed strong near-infrared (NIR) fluorescence caused by fluorescence resonance energy transfer (FRET) between PDA network and semiconducting SWCNT core. The micro-patterning of biotinylated PDA/SWCNT with FITC-avidin on biotinylated glass surface demonstrated the potential application for a bio-sensing device. Furthermore, the biocompatibility for mammalian cancer cells was tested by viability experiments, which revealed that the PDA/SWCNTs had very low toxicity below 31.3 mg/L in terms of pristine SWCNTs concentration. Also, PDA/SWCNTs inside the cells can be observed by NIR microscopy. This unique modular method of preparation can contribute to diverse functionalities for practical applications in various non-invasive cellular imaging.
authors.affiliation: BT Convergence Technology Research Department, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-530, Korea.
authors.forename: Kwang Suk
authors.initials: KS
authors.lastname: Yang
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Yun
authors.forename: Jin Chul
authors.initials: JC
authors.lastname: Kim
authors.forename: Jungsun
authors.initials: J
authors.lastname: Min
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Park
authors.forename: Jeong Keun
authors.initials: JK
authors.lastname: Ahn
authors.forename: Do Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D045744:Cell Line, Tumor; D003287:Contrast Media / Q000138:chemical synthesis*; D006801:Humans; D008853:Microscopy / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009369:Neoplasms / Q000473:pathology*; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D037742:Nanotubes, Carbon; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene