title: A cleanup method for mass spectrometry of sphingosine-1-phosphate in blood and solid tissues using a phosphate capture molecule.
abstract: Cleanup technology and mass spectrometric determination of sphingosine-1-phosphate using a -phosphate capture molecule are shown. The protocol is rapid, requires neither thin-layer chromatography nor liquid chromatography, and is applicable to both blood and solid tissue samples.
authors.affiliation: Research Center for Green Science, Fukuyama University, Fukuyama, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Morishige
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Satouchi
mesh_terms: D000818:Animals; D002417:Cattle; D008168:Lung / Q000737:chemistry; D008246:Lysophospholipids / Q000032:analysis* / Q000097:blood*; D010710:Phosphates / Q000032:analysis / Q000302:isolation & purification*; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood
chemical_list: D008246:Lysophospholipids; D010710:Phosphates; C060506:sphingosine 1-phosphate; D013110:Sphingosine