title: Inhibitory effect of 5,8,11-eicosatrienoic acid on angiogenesis.
abstract: BACKGROUND
  Cartilage contains high levels of n-9 eicosatrienoic acid (20:3n-9) but no blood vessels. 20:3n-9 might inhibit angiogenesis.
  
  
  METHODS
  Angiogenesis was measured in human umbilical vein endothelial cells and diploid fibroblasts. Co-culture was performed with vascular endothelial growth factor-A (VEGF-A, 10 ng/mL) and fatty acids (0.1-10 μmol/L). After 10 days of incubation and immunostaining for endothelial cells, vessel areas were calculated with image analyser software.
  
  
  RESULTS
  Addition of 20:3n-9 and n-3 eicosatrienoic acid (20:3n-3) dose dependently inhibited VEGF-A-stimulated angiogenesis (more than the positive control suramin). Arachidonic, eicosapentaenoic, dihomo-γ-linolenic (20:3n-6) and oleic acids did not affect VEGF-A-stimulated angiogenesis even at 10 μmol/L. Arachidonic and dihomo-γ-linolenic acids enhanced angiogenesis without VEGF-A.
  
  
  CONCLUSIONS
  We suggest that the presence of 20:3n-9 in cartilage may be related to its vessel-free status and that 20:3n-9 may be useful for the treatment of disorders with excessive vasculature.
  
  
  BACKGROUND
  This work was partly supported by Polyene Project, Inc.
authors.affiliation: Department of Clinical Sciences, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama City, Toyama, Japan. hamazaki@inm.u-toyama.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Hamazaki
authors.forename: T
authors.initials: T
authors.lastname: Nagasawa
authors.forename: K
authors.initials: K
authors.lastname: Hamazaki
authors.forename: M
authors.initials: M
authors.lastname: Itomura
mesh_terms: D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism; D018920:Coculture Techniques; D015118:Eicosapentaenoic Acid / Q000378:metabolism / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D015126:8,11,14-Eicosatrienoic Acid; C010944:mead acid