title: Effects of different combinations of benzyl adenine and indole acetic acid concentrations on in vitro plant regeneration in hexaploid wheat.
abstract: Development of a reliable in vitro plant regeneration procedure for hexaploid bread wheat (Triticum aestivum L.) is a prerequisite for its improvement by genetic transformation. Here, we report the effects of two growth regulators, benzyl adenine (BA) and indole acetic acid (IAA) on callus induction and plant regeneration from scutellum cultures of two commercial bread wheat cultivars: Giza 164 and Sakha 69. Callus induction was obtained from isolated embryos cultured on modified Murashige and Skoog (MS) basal medium. After four weeks of callus induction, all calli were plated on MS basal medium for regeneration. Wheat genotype and callus induction medium played a dominant role in plantlet regeneration. 2.0 mg/L BA and 0.2 mg/L IAA were the best combinations for inducing callus and let to highest regeneration frequency (81.67%) across the cultivars. Overall, based on our medium conditions, Giza 164 displayed higher regeneration frequency (81.11%) than Sakha 69. These results will facilitate genetic transformation for the economic varieties Giza 164 and Sakha 69.
authors.affiliation: Agriculture Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt. ashrafhussien55@yahoo.com
authors.forename: Ashraf H
authors.initials: AH
authors.lastname: Fahmy
authors.forename: Jiarui
authors.initials: J
authors.lastname: Li
authors.forename: Walaa
authors.initials: W
authors.lastname: Abou El-Wafa
authors.forename: Soheir El-Saeed
authors.initials: Sel-S
authors.lastname: El-Khodary
authors.forename: Osama M
authors.initials: OM
authors.lastname: El Shihy
mesh_terms: D001593:Benzyl Compounds; D004305:Dose-Response Relationship, Drug; D005838:Genotype; D007210:Indoleacetic Acids / Q000494:pharmacology*; D007701:Kinetin / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000235:genetics / Q000502:physiology; D011123:Polyploidy; D011687:Purines; D012038:Regeneration / Q000187:drug effects*; D013045:Species Specificity; D046509:Tissue Culture Techniques; D014908:Triticum / Q000145:classification / Q000235:genetics / Q000502:physiology*
chemical_list: D001593:Benzyl Compounds; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; C030737:indoleacetic acid; C480551:benzylaminopurine; D007701:Kinetin