title: Phospholipid profiling of 57 soybean (Glycine max) varieties by high-performance liquid chromatography-tandem mass spectrometry and principal component analysis to classify Korean soybean germplasm.
abstract: Phospholipids (PLs) in 57 varieties of soybeans were profiled by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry and principal component analysis (PCA) to discriminate PL-rich soybeans. The PL calibration curves showed linearity with correlation coefficients >0.9964. The recoveries at 5 mg/L spiked level ranged from 72.8 to 86.7% and those at 12.5 mg/L from 78.2 to 85.1%. The repeatability at a 5 mg/L spiked level ranged from 2.5 to 7.0% and those at 12.5 mg/L from 1.2 to 3.9%. The average total PL content in the 57 soybean varieties was about 35.3 mg/kg. The total PL content was the highest in Aodaiz (35, 48.7 ± 1.4 mg/kg) and the lowest in Poongsannamul (56, 16.0 ± 0.7 mg/kg). The PCA showed that RS-78sun (42), Gyeongsang #1 (3) and Aodaiz (35) are the most improved varieties of the investigated 57 varieties from the viewpoint of PL content.
authors.affiliation: Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Semin
authors.initials: S
authors.lastname: Park
authors.forename: Soo Taek
authors.initials: ST
authors.lastname: Kim
authors.forename: Chi Yeon
authors.initials: CY
authors.lastname: Kim
authors.forename: Yun Hi
authors.initials: YH
authors.lastname: Kim
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Jeong
authors.forename: Gon-Sup
authors.initials: GS
authors.lastname: Kim
authors.forename: Jong Il
authors.initials: JI
authors.lastname: Chung
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D056910:Republic of Korea; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010743:Phospholipids