title: Transient interactions of a slow-folding protein with the Hsp70 chaperone machinery.
abstract: Most known proteins have at least one local Hsp70 chaperone binding site. Does this mean that all proteins interact with Hsp70 as they fold? This study makes an initial step to address the above question by examining the interaction of the E.coli Hsp70 chaperone (known as DnaK) and its co-chaperones DnaJ and GrpE with a slow-folding E.coli substrate, RNase H(D). Importantly, this protein is a nonobligatory client, and it is able to fold in vitro even in the absence of chaperones. We employ stopped-flow mixing, chromatography, and activity assays to analyze the kinetic perturbations induced by DnaK/DnaJ/GrpE (K/J/E) on the folding of RNase H(D). We find that K/J/E slows down RNase H(D)'s apparent folding, consistent with the presence of transient chaperone-substrate interactions. However, kinetic retardation is moderate for this slow-folding client and it is expected to be even smaller for faster-folding substrates. Given that the interaction of folding-competent substrates such as RNase H(D) with the K/J/E chaperones is relatively short-lived, it does not significantly interfere with the timely production of folded biologically active substrate. The above mode of action is important because it preserves K/J/E bioavailability, enabling this chaperone system to act primarily by assisting the folding of other misfolded and (or) aggregation-prone cellular proteins that are unable to fold independently. When refolding is carried out in the presence of K/J and absence of the nucleotide exchange factor GrpE, some of the substrate population becomes trapped as a chaperone-bound partially unfolded state.
authors.affiliation: Biophysics Program, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Ashok
authors.initials: A
authors.lastname: Sekhar
authors.forename: Margarita
authors.initials: M
authors.lastname: Santiago
authors.forename: Hon Nam
authors.initials: HN
authors.lastname: Lam
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Silvia
authors.initials: S
authors.lastname: Cavagnero
mesh_terms: D002850:Chromatography, Gel; D056148:Chromatography, Reverse-Phase; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D050956:HSP40 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism; D017510:Protein Folding; D016914:Ribonuclease H / Q000737:chemistry / Q000378:metabolism*
chemical_list: C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; C489400:GrpE protein, E coli; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D016914:Ribonuclease H; C063842:dnaK protein, E coli