title: SIRT1 promotes tumorigenesis of hepatocellular carcinoma through PI3K/PTEN/AKT             signaling.
abstract: SIRT1 is the human orthologue of SIR2, a conserved NAD-dependent protein             deacetylase that regulates longevity in yeast and in Caenorhabditis elegans. Overexpression             of SIRT1 in cancer tissue, compared with normal tissue, has been demonstrated,             suggesting that SIRT1 may act as a tumor promoter. The function of SIRT1 in liver             cancer has not been elucidated. In the present study, SIRT1 re-expression or knockdown             was induced in hepatoma cell lines and liver normal cell lines. Our study demonstrated             that overexpression of SIRT1 promoted mitotic entry of liver cells, cell growth             and proliferation and inhibited apoptosis. The apoptosis involved caspase-3 and             caspase-7, and was related to the PTEN/PI3K/AKT signaling pathway. The results             demonstrate that SIRT1 promotes tumorigenesis of hepatocellular carcinoma (HCC)             through the PTEN/PI3K/AKT signaling pathway. SIRT1 may serve as a novel target             for selective killing of cancer versus normal liver cells.
authors.affiliation: Department of General Surgery, the Second People's Hospital of Guangdong Province, Guangzhou 510317, PR China.
authors.forename: Hanning
authors.initials: H
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Kaiyun
authors.initials: K
authors.lastname: Chen
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Xiao
authors.forename: Ke
authors.initials: K
authors.lastname: He
authors.forename: Jinqian
authors.initials: J
authors.lastname: Zhang
authors.forename: Guoan
authors.initials: G
authors.lastname: Xiang
mesh_terms: D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D059585:G1 Phase Cell Cycle Checkpoints; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D008938:Mitosis; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; C505407:CASP3 protein, human; C505520:CASP7 protein, human; D053148:Caspase 3; D053179:Caspase 7; C447939:SIRT1 protein, human; D056564:Sirtuin 1