title: Differences in vanadocene dichloride and cisplatin effect on MOLT-4 leukemia and human peripheral blood mononuclear cells.
abstract: Modern chemotherapy is interested in developing new agents with high efficiency of treatment in low-dose medication strategies, lower side toxicity and stronger specificity to the tumor cells. Vanadocene dichloride (VDC) belongs to the group of the most promising metallocene antitumor agents; however, its mechanism of action and cytotoxicity profile are not fully understood. In this paper we assess cytotoxic effects of VDC in comparison to cisplatin using opposite prototype of cells; human peripheral blood mononuclear (PBMCs) cells and human acute lymphoblastic leukemia cell line (MOLT-4). Our findings showed cytotoxic effect of VDC on leukemia cells, but unfortunately on human peripheral blood mononuclear cells as well. VDC induces apoptosis in leukemia cells; the induction is, however, lower than that of cisplatin, and in contrary to cisplatin, VDC does not induce p53 up-regulation. Cytotoxic effect of VDC on leukemia cells is less pronounced than that of cisplatin and more pronounced in PBMCs than in MOLT-4 cells.
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Czech Republic.
authors.forename: Radim
authors.initials: R
authors.lastname: Havelek
authors.forename: Pavel
authors.initials: P
authors.lastname: Siman
authors.forename: Jana
authors.initials: J
authors.lastname: Cmielova
authors.forename: Alena
authors.initials: A
authors.lastname: Stoklasova
authors.forename: Jirina
authors.initials: J
authors.lastname: Vavrova
authors.forename: Jaromir
authors.initials: J
authors.lastname: Vinklarek
authors.forename: Jiri
authors.initials: J
authors.lastname: Knizek
authors.forename: Martina
authors.initials: M
authors.lastname: Rezacova
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000188:drug therapy; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D017968:Vanadium Compounds / Q000494:pharmacology*
chemical_list: D017968:Vanadium Compounds; C030754:vanadocene dichloride; D002945:Cisplatin