title: Biofilm formed by a hypervirulent (hypermucoviscous) variant of Klebsiella pneumoniae does not enhance serum resistance or survival in an in vivo abscess model.
abstract: A new hypervirulent (hypermucoviscous) clinical variant of Klebsiella pneumoniae (hvKP) has emerged over the last decade. Our goal is to identify new mechanisms, which increase the virulence hvKP. It has been shown that hvKP strains produce more biofilm than "classical" stains of K. pneumoniae, therefore we hypothesized that biofilm formation may contribute to the pathogenesis of systemic infection. To test this hypothesis, transposon mutants of the model pathogen hvKP1 were generated and screened for decreased production of biofilm. Three mutant constructs with disruptions in glnA [putatively encodes glutamine synthetase, hvKP1 glnA:: EZ::TN < KAN-2 > (glnA::Tn)], sucD [putatively encodes succinyl-CoA synthase α subunit, hvKP1 sucD:: EZ::TN < KAN-2 > (sucD::Tn)], and tag [putatively encodes transcriptional antiterminator of glycerol uptake operon, hvKP1 tag:: EZ::TN < KAN-2 > (tag::Tn)] were chosen for further characterization and use in biologic studies. Quantitative assays performed in rich laboratory medium and human ascites confirmed the phenotype and a hypermucoviscosity assay established that capsule production was not affected. However, compared with its wild-type parent, neither planktonic cells nor biofilms of glnA::Tn, sucD::Tn and tag::Tn displayed a change to the bactericidal activity of 90% human serum. Likewise, when assessed in a rat subcutaneous abscess model, the growth and survival of glnA::Tn, sucD::Tn and tag::Tn in abscess fluid was similar to hvKP1. In this report we identify three new genes that contribute to biofilm formation in hvKP1. However, decreased biofilm production due to disruption of these genes does not affect the sensitivity of these mutant constructs to 90% human serum when in planktonic form or within a biofilm. Further, their virulence in an in vivo abscess model was unaffected.
authors.affiliation: Department of Medicine, University of Buffalo-State University of New York, Buffalo, NY, USA.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Kong
authors.forename: Janet M
authors.initials: JM
authors.lastname: Beanan
authors.forename: Ruth
authors.initials: R
authors.lastname: Olson
authors.forename: Ulrike
authors.initials: U
authors.lastname: Macdonald
authors.forename: Alyssa S
authors.initials: AS
authors.lastname: Shon
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Metzger
authors.forename: Alexander O
authors.initials: AO
authors.lastname: Pomakov
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Russo
mesh_terms: D000038:Abscess / Q000382:microbiology*; D000818:Animals; D018441:Biofilms / Q000254:growth & development*; D001770:Blood Bactericidal Activity; D004251:DNA Transposable Elements; D004195:Disease Models, Animal; D055786:Gene Knockout Techniques; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology*; D008297:Male; D050296:Microbial Viability; D016254:Mutagenesis, Insertional; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D051381:Rats; D020318:Rats, Long-Evans; D044967:Serum / Q000276:immunology; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D004251:DNA Transposable Elements; D011135:Polysaccharides, Bacterial; D037521:Virulence Factors