title: [Pseudomonas aeruginosa vaccine inhibits the proliferation of human nasopharyngeal cancer cells in vitro].
abstract: OBJECTIVE
  To investigate the inhibitory effects of Pseudomonas aeruginosa vaccine (PA-MSHA) on the proliferation of human nasopharyngeal cancer cells and explore the possible mechanism.
  
  
  METHODS
  MTT assay was used to determine the cell growth of human nasopharyngeal cancer cell line 5-8F and 6-10B in vitro treated with the vaccine. The cell cycle distribution of the cells was detected by flow cytometry, and the expression levels of apoptosis and cycle-related proteins were evaluated by Western blotting.
  
  
  RESULTS
  PA-MSHA treatment significantly suppressed the proliferation of 5-8F and 6-10B cells in a time- and concentration-dependent manner compared with the control group (P<0.05). The cells with PA-MSHA treatment exhibited a decreased percentage of cells entering S phase and a corresponding increase in G(1) phase cells in FACS analysis. The expression of cyclin D(1), CDK4, and CDK6 was significantly up-regulated, Bax protein up-regulated, and the anti-apoptosis protein Bcl-2 down-regulated in PA-MSHA-treated cells.
  
  
  CONCLUSIONS
  PA-MSHA can suppress the proliferation of nasopharyngeal carcinoma cell in vitro by affecting the cell cycle and promoting cell apoptosis.
authors.affiliation: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. jimmy7198@gmail.com
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Dehua
authors.initials: D
authors.lastname: Wu
authors.forename: Longhua
authors.initials: L
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*
chemical_list: D019496:Cancer Vaccines