title: The modulation of PPARγ1 and PPARγ2 mRNA expression by ciglitazone in CD3/CD28-activated naïve and memory CD4+ T cells.
abstract: Given their roles in immune regulation, the expression of the nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) 1 and 2 isoforms was investigated in human naïve (CD45RA+) and memory (CD45RO+) CD4+ T cells. Stimulation of both types of cells via the CD3/CD28 pathway resulted in high expression of both PPARγ receptors as measured by real-time PCR. Treatment with the PPARγ agonist, ciglitazone, increased PPARγ1 expression but decreased PPARγ2 expression in stimulated naïve and memory cells. Furthermore, when present, the magnitude of both PPARγ receptors expression was lower in naïve cells, perhaps suggesting a lower regulatory control of these cells. Similar profiles of selected proinflammatory cytokines were expressed by the two cell types following stimulation. The induction of PPARγ1 and suppression of PPARγ2 expressions in naïve and memory CD4+ T cells in the presence of ciglitazone suggest that the PPARγ subtypes may have different roles in the regulation of T-cell function.
authors.affiliation: School of Health Sciences, Universiti Sains Malaysia, Kelantan, 16150 Kubang Kerian, Malaysia. norazmi@kb.usm.my
authors.forename: Mohd Nor
authors.initials: MN
authors.lastname: Norazmi
authors.forename: Rafeezul
authors.initials: R
authors.lastname: Mohamed
authors.forename: Asma Abdullah
authors.initials: AA
authors.lastname: Nurul
authors.forename: Nik Soriani
authors.initials: NS
authors.lastname: Yaacob
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007156:Immunologic Memory; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008863:Microspheres; D047495:PPAR gamma / Q000819:agonists* / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D045162:Thiazolidinediones / Q000494:pharmacology*
chemical_list: D015703:Antigens, CD; D007004:Hypoglycemic Agents; D047495:PPAR gamma; D012333:RNA, Messenger; D045162:Thiazolidinediones; C039671:ciglitazone