title: Optimal estimation of ion-channel kinetics from macroscopic currents.
abstract: Markov modeling provides an effective approach for modeling ion channel kinetics. There are several search algorithms for global fitting of macroscopic or single-channel currents across different experimental conditions. Here we present a particle swarm optimization(PSO)-based approach which, when used in combination with golden section search (GSS), can fit macroscopic voltage responses with a high degree of accuracy (errors within 1%) and reasonable amount of calculation time (less than 10 hours for 20 free parameters) on a desktop computer. We also describe a method for initial value estimation of the model parameters, which appears to favor identification of global optimum and can further reduce the computational cost. The PSO-GSS algorithm is applicable for kinetic models of arbitrary topology and size and compatible with common stimulation protocols, which provides a convenient approach for establishing kinetic models at the macroscopic level.
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Xiao
authors.forename: Xuhui
authors.initials: X
authors.lastname: Zeng
authors.forename: Jing
authors.initials: J
authors.lastname: Yao
authors.forename: Ming
authors.initials: M
authors.lastname: Yuchi
authors.forename: Jiuping
authors.initials: J
authors.lastname: Ding
mesh_terms: D000465:Algorithms; D000494:Allosteric Regulation; D000818:Animals; D003198:Computer Simulation; D007700:Kinetics; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D008390:Markov Chains; D008564:Membrane Potentials; D008958:Models, Molecular; D009865:Oocytes / Q000378:metabolism; D018408:Patch-Clamp Techniques; D014981:Xenopus
chemical_list: D051036:Large-Conductance Calcium-Activated Potassium Channels