title: Preparation, characterization, and surface immobilization of native vesicles obtained by mechanical extrusion of mammalian cells.
abstract: Native vesicles or "reduced protocells" derived by mechanical extrusion concentrate selected plasma membrane components, while downsizing complexities of whole cells. We illustrate this technique, characterize the physical-chemical properties of these reduced configurations of whole cells, and demonstrate their surface immobilization and patternability. This simple detergent-free vesicularized membrane preparation should prove useful in fundamental studies of cellular membranes, and may provide a means to engineer therapeutic cells and enable high-throughput devices containing near-native, functional proteolipidic assemblies.
authors.affiliation: Department of Applied Science, University of California, Davis, One Shields Avenue, Davis, California 95616, USA.
authors.forename: Huawen
authors.initials: H
authors.lastname: Wu
authors.forename: Ann E
authors.initials: AE
authors.lastname: Oliver
authors.forename: Viviane N
authors.initials: VN
authors.lastname: Ngassam
authors.forename: Chanel K
authors.initials: CK
authors.lastname: Yee
authors.forename: Atul N
authors.initials: AN
authors.lastname: Parikh
authors.forename: Yin
authors.initials: Y
authors.lastname: Yeh
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D008856:Microscopy, Fluorescence; D013059:Spectrum Analysis, Raman; D053835:Unilamellar Liposomes / Q000378:metabolism*
chemical_list: D053835:Unilamellar Liposomes