title: [Pathological findings of axonal injury in a rat model of experimental allergic encephalomyelitis].
abstract: OBJECTIVE
  To observe the pathological changes of axonal injury in a rat model of experimental allergic encephalomyelitis (EAE).
  
  
  METHODS
  With HE, luxol fast blue and Bielschowsky staining, the expression of APP, MBP, SMI-32 and MBP in the brain and spinal cord of EAE rats using double-labeling indirect immunofluorescence.
  
  
  RESULTS
  Extensive cuffing lesions of inflammatory cell infiltrations were found in the brain and spinal cord of the rats, accompanied by multiple lesions of demyelination, axonal disarrangement with vesicular loss. SMI-32 staining identified numerous nonphosphorylated neurofilament, indicating the presence of axonal injury. Axonal oval bodies formed by APP accumulation were found in the white matters of the spinal cord 14 days after EAE, suggesting that neuraxial damage occurred in the early stage of EAE which was not synchronous with myelin loss.
  
  
  CONCLUSIONS
  Different levels of inflammation occur in different stages of EAE, and inflammatory cell infiltration is the most obvious at the peak of EAE. Axonal injury occurs in the early stage of EAE and progresses over the entire disease course.
authors.affiliation: Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. yuanyuanlisia@126.com
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Tianming
authors.initials: T
authors.lastname: Lv
authors.forename: Xiaojia
authors.initials: X
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Fang
authors.forename: Yanshan
authors.initials: Y
authors.lastname: Liang
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology*; D001921:Brain / Q000473:pathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology*; D005260:Female; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000473:pathology*