title: Smooth muscle relaxant evaluation of Jatropha curcas Linn (Euphorbiaceae) and isolation of triterpenes.
abstract: Jatropha curcas is a herbal preparation used in the tropics for the treatment of threatened abortion and related problems associated with pregnancy. The Stem bark of Jatropha curcas is used ethno medicinally in Nigeria especially in the eastern part of the country for the treatment of infertility and spontaneous abortion (miscarriage). The present study was undertaken in order to validate the folkloric claim, using scientific experimental procedures and bioassay guided fractionation. The crude powdered sample was subjected to phytochemical screening testing for the presence of alkaloids, tannins, saponins and carbohydrates. Chromatographic analysis (TLC and VLC) were carried out using various solvent systems. The effect of methanolic extracts on rat uterine contractions was studied in vitro, in 40ml organ baths containing physiological salt solution of De Jalon maintained at 370C, aerated with 95% O2 and 5% CO2 with an isometric transducer connected an UgoBasile recorder under a resting tension of 750mg. The result of the phytochemical screening revealed the presence of glycosides, tannins, saponins and alkaloids. The extract abolished significantly the spontaneous contraction of the uterus and reduced acetylcholine induced uterine contractions at a dose of 50mg/ml. The tocolytic effects indicate the presence of active principle(s) which would explain the ethno medicinal use of the stem bark of Jatropha curcas to treat spontaneous abortion.
authors.affiliation: . faloabi@uniben.edu.
authors.forename: Abiodun
authors.initials: A
authors.lastname: Falodun
authors.forename: Z A M
authors.initials: ZA
authors.lastname: Nworgu
authors.forename: Erharuyi
authors.initials: E
authors.lastname: Osayemwenre
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002855:Chromatography, Thin Layer; D005260:Female; D066298:In Vitro Techniques; D007537:Isometric Contraction; D031286:Jatropha / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D016488:Medicine, African Traditional; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009549:Nigeria; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents; D015149:Tocolytic Agents / Q000494:pharmacology; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D014590:Uterine Contraction / Q000187:drug effects
chemical_list: D010936:Plant Extracts; D012997:Solvents; D015149:Tocolytic Agents; D014315:Triterpenes; D000109:Acetylcholine