title: Treatment of experimental periodontitis in rats using repeated adjunctive antimicrobial photodynamic therapy.
abstract: The aim of this study was to histologically and histometrically evaluate the influence of repeated adjunctive antimicrobial photodynamic therapy (aPDT) on bone loss (BL) in furcation areas in rats. Periodontitis was induced by placing a ligature around the mandibular molar in 75 rats. The animals were divided into five groups: the SS group was treated with saline solution (SS); the SRP group received scaling and root planing (SRP); the aPDT1 group received SRP as well as toluidine blue (TBO) and low-level laser therapy (LLLT; InGaAlP, 660 nm; 4.94 J/cm(2)/point) postoperatively at 0 h; the aPDT2 group received SRP as well as TBO and LLLT postoperatively at 0, 24, 28, and 72 h; and the aPDT3 group received SRP, TBO, and LLLT postoperatively at 0, 48, 96, and 144 h. The area of BL in the furcation region of the molar was histometrically analyzed. Data were analyzed statistically (P < 0.05). Animals treated with a single episode of aPDT showed less BL at days 7 and 30 than those who received only SRP treatment. No significant differences were found among the aPDT groups (P > 0.05). Repeated aPDT did not improve BL reduction when compared to a single episode of aPDT.
authors.affiliation: GEPLO-Group of Study and Research of Lasers in Dentistry, Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil. vg.garcia@uol.com.br
authors.forename: Valdir Gouveia
authors.initials: VG
authors.lastname: Garcia
authors.forename: Mariéllen
authors.initials: M
authors.lastname: Longo
authors.forename: Leandro Araújo
authors.initials: LA
authors.lastname: Fernandes
authors.forename: Erivan Clementino
authors.initials: EC
authors.lastname: Gualberto
authors.forename: Carolina dos Santos
authors.initials: Cdos S
authors.lastname: Santinoni
authors.forename: Alvaro Francisco
authors.initials: AF
authors.lastname: Bosco
authors.forename: Maria José Hitomi
authors.initials: MJ
authors.lastname: Nagata
authors.forename: Letícia Helena
authors.initials: LH
authors.lastname: Theodoro
mesh_terms: D016301:Alveolar Bone Loss; D000704:Analysis of Variance; D000818:Animals; D012534:Dental Scaling; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D010518:Periodontitis / Q000382:microbiology / Q000532:radiotherapy*; D051381:Rats; D017208:Rats, Wistar; D016745:Root Planing; D012965:Sodium Chloride / Q000494:pharmacology; D014048:Tolonium Chloride / Q000494:pharmacology
chemical_list: D014048:Tolonium Chloride; D012965:Sodium Chloride