title: Study of neurotoxic intracellular calcium signalling triggered by amyloids.
abstract: Neurotoxicity in Alzheimer's disease (AD) is associated to dishomeostasis of intracellular Ca(2+) induced by amyloid β peptide (Aβ) species. Understanding of the effects of Aβ on intracellular Ca(2+) homeostasis requires preparation of the different Aβ assemblies including oligomers and fibrils and the testing of their effects on cytosolic and mitochondrial Ca(2+) in neurons. Procedures for cerebellar granule cell culture, preparation of Aβ species as well as fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca(2+) in neurons are described.
authors.affiliation: Instituto de Biología y Genética Molecular (IBGM), Universidad de Valladolid and Consejo Superior de Investigaciones Científicas (CSIC), Valladolid, Spain. carlosv@ibgm.uva.es
authors.forename: Carlos
authors.initials: C
authors.lastname: Villalobos
authors.forename: Erica
authors.initials: E
authors.lastname: Caballero
authors.forename: Sara
authors.initials: S
authors.lastname: Sanz-Blasco
authors.forename: Lucía
authors.initials: L
authors.lastname: Núñez
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects*; D002478:Cells, Cultured; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D016257:Fura-2 / Q000378:metabolism; D042541:Intracellular Space / Q000187:drug effects* / Q000378:metabolism*; D008163:Luminescent Measurements; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D057054:Molecular Imaging; D009474:Neurons / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000633:toxicity*; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D002118:Calcium; D016257:Fura-2