title: Genotype-dependent sulphite tolerance of Australian Dekkera (Brettanomyces) bruxellensis wine isolates.
abstract: OBJECTIVE
  The aim of this study was to determine sulphite tolerance for a large number of Dekkera bruxellensis isolates and evaluate the relationship between this phenotype and previously assigned genotype markers.
  
  
  RESULTS
  A published microplate-based method for evaluation of yeast growth in the presence of sulphite was benchmarked against culturability following sulphite treatment, for the D. bruxellensis type strain (CBS 74) and a reference wine isolate (AWRI 1499). This method was used to estimate maximal sulphite tolerance for 41 D. bruxellensis isolates, which was found to vary over a fivefold range. Significant differences in sulphite tolerance were observed when isolates were grouped according to previously assigned genotypes and ribotypes.
  
  
  CONCLUSIONS
  Variable sulphite tolerance for the wine spoilage yeast D. bruxellensis can be linked to genotype markers.
  
  
  CONCLUSIONS
  Strategies to minimize risk of wine spoilage by D. bruxellensis must take into account at least a threefold range in effective sulphite concentration that is dependent upon the genotype group(s) present. The isolates characterized in this study will be a useful resource for establishing the mechanisms conferring sulphite tolerance for this industrially important yeast species.
authors.affiliation: The Australian Wine Research Institute, Glen Osmond, Adelaide, SA, Australia. chris.curtin@awri.com.au
authors.forename: C
authors.initials: C
authors.lastname: Curtin
authors.forename: E
authors.initials: E
authors.lastname: Kennedy
authors.forename: P A
authors.initials: PA
authors.lastname: Henschke
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001315:Australia; D055168:Brettanomyces / Q000187:drug effects / Q000235:genetics; D055167:Dekkera / Q000187:drug effects / Q000235:genetics*; D005838:Genotype; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D013447:Sulfites / Q000494:pharmacology*; D014920:Wine / Q000382:microbiology*
chemical_list: D013447:Sulfites