title: A novel bridge between oxidative stress and immunity: the interaction between hydrogen peroxide and human leukocyte antigen G in placental trophoblasts during preeclampsia.
abstract: OBJECTIVE
  The aim of this study was to investigate a possible association between hydrogen peroxide and human leukocyte antigen G (HLA-G) in preeclampsia.
  
  
  METHODS
  We explored the correlation between HLA-G and hydrogen peroxide in preeclamptic (n = 30) and normotensive (n = 30) placentas, which was confirmed by in vitro experiments. Furthermore, RNA interference was used to explore the influence of HLA-G on the proliferation, apoptosis and invasion of HTR-8/SVneo cells when exposed to hydrogen peroxide.
  
  
  RESULTS
  We found an inverse correlation between hydrogen peroxide and HLA-G expression in preeclamptic placentas. High levels of hydrogen peroxide could down-regulate HLA-G expression in HTR-8/SVneo. Compared with HLA-G knockdown HTR-8/SVneo, increased proliferation inhibition, higher apoptosis, and decreased cell invasion were found in the cell expressing HLA-G when exposed to hydrogen peroxide.
  
  
  CONCLUSIONS
  Our findings highlight that high levels of hydrogen peroxide can down-regulate HLA-G expression in trophoblasts during preeclampsia and trophoblasts expressing HLA-G are vulnerable to oxidative stress.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.forename: Guo-ying
authors.initials: GY
authors.lastname: Zhang
authors.forename: Jue
authors.initials: J
authors.lastname: Wang
authors.forename: Shou-lian
authors.initials: SL
authors.lastname: Lu
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Li-zhou
authors.initials: LZ
authors.lastname: Sun
mesh_terms: D000328:Adult; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D016022:Case-Control Studies; D002460:Cell Line; D002470:Cell Survival; D015536:Down-Regulation; D005260:Female; D005455:Fluorescent Antibody Technique; D059951:HLA-G Antigens / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D018384:Oxidative Stress; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000209:etiology* / Q000276:immunology; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000378:metabolism / Q000502:physiology
chemical_list: D015415:Biomarkers; D059951:HLA-G Antigens; D006861:Hydrogen Peroxide