title: A rapid mismatch polymerase chain reaction assay to detect carriers of complex vertebral malformation in Holstein cattle.
abstract: Three Holstein sires were identified as complex vertebral malformation (CVM) carriers using the polymerase chain reaction-primer introduced restriction analysis (PCR-PIRA) method. Using the carriers as positive controls, the PCR mismatch amplification mutation assay (MAMA PCR) method was developed and validated by sequence analysis. With MAMA PCR, 154 Chinese Holstein sires were tested for CVM, among which 24 were confirmed to be CVM carriers. With DNA isolated from hair follicles, 10 daughters of one CVM-positive bull were detected, among which 7 were confirmed to be CVM carriers.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Haisheng
authors.initials: H
authors.lastname: Hao
authors.forename: Xueming
authors.initials: X
authors.lastname: Zhao
authors.forename: Huabin
authors.initials: H
authors.lastname: Zhu
authors.forename: Weihua
authors.initials: W
authors.lastname: Du
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Tong
authors.initials: T
authors.lastname: Qin
authors.forename: Zongli
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics* / Q000473:pathology; D002574:Cervical Vertebrae / Q000002:abnormalities*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005260:Female; D006580:Genetic Carrier Screening / Q000379:methods*; D008297:Male; D017354:Point Mutation; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D013122:Spinal Diseases / Q000235:genetics / Q000473:pathology / Q000662:veterinary*
chemical_list: D004247:DNA