title: Kinetic and solvent deuterium isotope effects in the oxidation of putrescine catalysed by enzyme diamine oxidase.
abstract: In this study, the kinetic isotope effects and solvent isotope effects in the reaction of the deamination of [(1R)-(2)H ] putrescine--catalysed by enzyme diamine oxidase (EC 1.4.3.6)--were determined using a non-competitive spectroscopic method. Putrescine, stereospecifically labelled with deuterium, was obtained by enzymatic decarboxylation of l-ornithine that was carried out in a fully deuteriated incubation medium.
authors.affiliation: Department of Chemistry, University of Warsaw, Pasteur 1 str, Warsaw 02-093, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pałka
authors.forename: Jolanta
authors.initials: J
authors.lastname: Szymańska
authors.forename: Marianna
authors.initials: M
authors.lastname: Kańska
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry*; D000641:Ammonia / Q000737:chemistry; D002384:Catalysis; D003903:Deuterium / Q000737:chemistry*; D007700:Kinetics; D000432:Methanol / Q000737:chemistry; D010084:Oxidation-Reduction; D011700:Putrescine / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D012997:Solvents; D000641:Ammonia; D003903:Deuterium; D006631:Amine Oxidase (Copper-Containing); D011700:Putrescine; D000432:Methanol