title: Two-dimensional gel-based alkaline proteome of the probiotic bacterium Lactobacillus acidophilus NCFM.
abstract: Lactobacillus acidophilus NCFM (NCFM) is a well-documented probiotic bacterium isolated from human gut. Detailed 2D gel-based NCFM proteomics addressed the so-called alkaline range, i.e., pH 6-11. Proteins were identified in 150 of the 202 spots picked from the Coomassie Brilliant Blue stained 2D gel using MALDI-TOF-MS. The 102 unique gene products among the 150 protein identifications were assigned to different functional categories, and evaluated by considering a calculated distribution of abundance as well as grand average of hydrophobicity values. None of the very few available lactic acid bacteria proteome reference maps included the range of pI >7.0. The present report of such data on the proteome of NCFM fundamentally complements current knowledge on protein profiles limited to the acid and neutral pH range.
authors.affiliation: Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark.
authors.forename: Avishek
authors.initials: A
authors.lastname: Majumder
authors.forename: Liyang
authors.initials: L
authors.lastname: Cai
authors.forename: Morten
authors.initials: M
authors.lastname: Ejby
authors.forename: Bjarne G
authors.initials: BG
authors.lastname: Schmidt
authors.forename: Sampo J
authors.initials: SJ
authors.lastname: Lahtinen
authors.forename: Susanne
authors.initials: S
authors.lastname: Jacobsen
authors.forename: Birte
authors.initials: B
authors.lastname: Svensson
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007779:Lactobacillus acidophilus / Q000737:chemistry*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome