title: Characterization of multi-functional properties and conformational analysis of MutS2 from Thermotoga maritima MSB8.
abstract: The MutS2 homologues have received attention because of their unusual activities that differ from those of MutS. In this work, we report on the functional characteristics and conformational diversities of Thermotoga maritima MutS2 (TmMutS2). Various biochemical features of the protein were demonstrated via diverse techniques such as scanning probe microscopy (SPM), ATPase assays, analytical ultracentrifugation, DNA binding assays, size chromatography, and limited proteolytic analysis. Dimeric TmMutS2 showed the temperature-dependent ATPase activity. The non-specific nicking endonuclease activities of TmMutS2 were inactivated in the presence of nonhydrolytic ATP (ADPnP) and enhanced by the addition of TmMutL. In addition, TmMutS2 suppressed the TmRecA-mediated DNA strand exchange reaction in a TmMutL-dependent manner. We also demonstrated that small-angle X-ray scattering (SAXS) analysis of dimeric TmMutS2 exhibited nucleotide- and DNA-dependent conformational transitions. Particularly, TmMutS2-ADPnP showed the most compressed form rather than apo-TmMutS2 and the TmMutS2-ADP complex, in accordance with the results of biochemical assays. In the case of the DNA-binding complexes, the stretched conformation appeared in the TmMutS2-four-way junction (FWJ)-DNA complex. Convergences of biochemical- and SAXS analysis provided abundant information for TmMutS2 and clarified ambiguous experimental results.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyungbuk, South Korea.
authors.forename: Euiyoung
authors.initials: E
authors.lastname: Jeong
authors.forename: Hunho
authors.initials: H
authors.lastname: Jo
authors.forename: Tae Gyun
authors.initials: TG
authors.lastname: Kim
authors.forename: Changill
authors.initials: C
authors.lastname: Ban
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics; D059765:Homologous Recombination; D006867:Hydrolases / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation; D053838:Scattering, Small Angle; D020124:Thermotoga maritima / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006867:Hydrolases; D000251:Adenosine Triphosphatases