title: Polymorphisms of the ATP1A1 gene associated  with mastitis in dairy cattle.
abstract: Mastitis affects the concentrations of potassium and  sodium in milk. Since sodium-potassium adenosine triphosphatase (Na(+), K(+)-ATPase) is critical for maintaining the  homeostasis of these two ions, and is involved in cell apoptosis and  pathogenesis, we presumed that polymorphism of the ATP1A1 gene, which  encodes the bovine Na(+), K(+)-ATPase α1 subunit could be associated with  mastitis. The ATP1A1 gene was analyzed in 320 Holstein cows using PCR  low ionic strength single-strand conformation polymorphism (PCR-LIS-SSCP) and  DNA sequencing methods. A C/A SNP was identified at nucleotide position -15,739  in exon 17 of the ATP1A1 gene, but it did not induce any change in amino  acids. We examined a possible association of polymorphism of the ATP1A1 gene  with somatic cell score and 305-day milk yields. Individuals with genotype CC in ATP1A1 had significantly lower somatic cell scores and 305-day  milk yields than those with genotype CA. We also examined changes in Na(+), K(+)-ATPase activity of red cell membranes.  The Na(+), K(+)-ATPase activity was significantly higher in dairy cows with genotype CC compared to the other two genotypes, and the Na(+), K(+)-ATPase activity of the resistant group  was significantly higher than that of the susceptible group in dairy cows. We  conclude that this polymorphism has potential as a marker for mastitis  resistance in dairy cattle.
authors.affiliation: College of Basic Medical Sciences, Henan University of Traditional Chinese Medicine, Zhengzhou, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Liu
authors.forename: C H
authors.initials: CH
authors.lastname: Xu
authors.forename: T Y
authors.initials: TY
authors.lastname: Gao
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D004789:Enzyme Activation; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D008414:Mastitis, Bovine / Q000201:enzymology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000378:metabolism
chemical_list: D000254:Sodium-Potassium-Exchanging ATPase