title: Comparative study of the effect of baicalin and its natural analogs on neurons with oxygen and glucose deprivation involving innate immune reaction of TLR2/TNFα.
abstract: This work is to study the baicalin and its three analogs, baicalin, wogonoside, and wogonin, on the protective effect of neuron from oxygen-glucose deprivation (OGD) and toll-like receptor 2 (TLR2) expression in OGD damage. The results showed that baicalin and its three analogs did protect neurons from OGD damage and downregulated protein level of TLR2. D-Glucopyranosiduronic acid on site 7 in the structure played a core of cytotoxicity of these flavonoid analogs. The methoxyl group on carbon 8 of the structure had the relation with TLR2 protein expression, as well as the anti-inflammation. In addition, we detected caspase3 and antioxidation capability, to investigate the effect of four analogs on cell apoptosis and total antioxidation competence in OGD model.
authors.affiliation: Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhao
authors.forename: Zhi-Yi
authors.initials: ZY
authors.lastname: Yuan
authors.forename: Hong-Jiao
authors.initials: HJ
authors.lastname: Wan
authors.forename: Fan
authors.initials: F
authors.lastname: Lei
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Xing
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Du
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D044950:Flavanones / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D005960:Glucosides / Q000494:pharmacology; D007113:Immunity, Innate / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D051195:Toll-Like Receptor 2 / Q000276:immunology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D051195:Toll-Like Receptor 2; D014409:Tumor Necrosis Factor-alpha; C038044:baicalin; C006680:baicalein; C505410:Casp3 protein, rat; D053148:Caspase 3; C473995:wogonoside; D005947:Glucose; C085514:wogonin; D010100:Oxygen