title: Enhanced cellular immune response elicited by a DNA vaccine fused with Ub against Mycobacterium tuberculosis.
abstract: This study evaluated the immune response elicited by a Ub-fused Ag85A DNA vaccine against Mycobacterium tuberculosis. BALB/c mice were vaccinated with plasmid DNA encoding Ag85A protein, Ub-fused Ag85A DNA vaccine (UbGR-Ag85A) and negative DNA vaccines, respectively. Ag85A DNA vaccine immunization induced a Th(l)-polarized immune response. The production of Th(l)-type cytokine (IFN-γ) and proliferative T cell responses was enhanced significantly in mice immunized with UbGR-Ag85A fusion DNA vaccine, compared with non-fusion DNA vaccine. Moreover, this fusion DNA vaccine also resulted in an increased relative ratio of IgG(2a) to IgG(l) and the cytotoxicity of T cells. IFN-γ intracellular staining of splenocytes indicated that UbGR-Ag85A fusion DNA vaccine activated CD4(+) and CD8(+) T cells, particularly CD8(+) T cells. Thus, this study demonstrated that the UbGR-Ag85A fusion DNA vaccine inoculation could improve antigen-specific cellular immune responses, which is helpful for protection against TB infection.
authors.affiliation: The Division of aviation medicine, Institute of Naval Medical Research, Shanghai, China. wqqmm_888@ yahoo.com
authors.forename: Q-M
authors.initials: QM
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
authors.forename: Ch-X
authors.initials: ChX
authors.lastname: Lei
authors.forename: F-Zh
authors.initials: FZ
authors.lastname: Shi
authors.forename: Q-H
authors.initials: QH
authors.lastname: Liu
mesh_terms: D000818:Animals; D005260:Female; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000276:immunology; D042541:Intracellular Space / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
chemical_list: D025801:Ubiquitin; D019444:Vaccines, DNA; D007371:Interferon-gamma