title: Impairment of α(2)-macroglobulin synthesis in experimental hepatopathic rats treated with turpentine oil.
abstract: The aim of this study was to investigate the synthesis of α(2)-macroglobulin (α2M) in hepatopathic rats injected with turpentine oil to induce acute inflammation. Hepatopathy was induced by oral administration of acetaminophen at a dose of 1 g/kg daily for 2 weeks or a 25% solution of carbon tetrachloride (CCl(4)) at 2 ml/kg body weight three times per week for 7 weeks. Acute inflammation was induced by intramuscular injection of turpentine oil at a dose of 1.0 ml/kg body weight. Serum concentrations of α2M were measured by enzyme-linked immunosorbent assay. Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and total protein differed significantly between acetaminophen or CCl(4)-induced hepatopathic rats and acetaminophen control (AA-control) or CCl(4) control (CC-control) rats. Furthermore, pathological examination confirmed hepatopathy in rat livers. Peak serum concentrations and area under the time-concentration curve for α2M showed significant differences between hepatopathic rats and AA-control or CC-control rats. Thus, serum concentrations of α2M did not increase when compared with nontreated rats.
authors.affiliation: Laboratory of Immunology, School of Life and Environmental Science, Azabu University, Kanagawa 252-5201, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuribayashi
authors.forename: Testuro
authors.initials: T
authors.lastname: Seita
authors.forename: Toshio
authors.initials: T
authors.lastname: Honjo
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Eiichi
authors.initials: E
authors.lastname: Momotani
authors.forename: Shizuo
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000082:Acetaminophen / Q000633:toxicity; D000208:Acute Disease; D018712:Analgesics, Non-Narcotic / Q000633:toxicity; D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D007273:Injections, Intramuscular; D007509:Irritants / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014425:Turpentine / Q000008:administration & dosage / Q000494:pharmacology*; D000511:alpha-Macroglobulins / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D018712:Analgesics, Non-Narcotic; D007509:Irritants; D000511:alpha-Macroglobulins; D000082:Acetaminophen; D002251:Carbon Tetrachloride; D014425:Turpentine