title: A marine-derived Streptomyces sp. MS449 produces high yield of actinomycin X2 and actinomycin D with potent anti-tuberculosis activity.
abstract: In the course of our screening program for anti-Mycobacterium bovis bacillus Calmette-Guérin (BCG) and anti-Mycobacterium tuberculosis H37Rv (MTB H37Rv) agents from our marine natural product library, a newly isolated actinomycete strain, designated as MS449, was picked out for further investigation. The strain MS449, isolated from a sediment sample collected from South China Sea, produced actinomycin X(2) and actinomycin D in substantial quantities, which showed strong inhibition of BCG and MTB H37Rv. The structures of actinomycins were elucidated by nuclear magnetic resonance and mass spectrometric analysis. The strain MS449 was taxonomically characterized on the basis of morphological and phenotypic characteristics, genotypic data, and phylogenetic analysis. The 16S rRNA gene sequence of the strain was determined and a database search indicated that the strain was closely associated with the type strain of Streptomyces avermitilis (99.7 % 16S rRNA gene similarity). S. avermitilis has not been previously reported to produce actinomycins. The marine-derived strain of Streptomyces sp. MS449 produced notably higher quantities of actinomycin X(2) (1.92 mg/ml) and actinomycin D (1.77 mg/ml) than previously reported actinomycins producing strains. Thus, MS449 was considered of great potential as a new industrial producing strain of actinomycin X(2) and actinomycin D.
authors.affiliation: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Chen
authors.forename: Fuhang
authors.initials: F
authors.lastname: Song
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Wael M
authors.initials: WM
authors.lastname: Abdel-Mageed
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Chengzhang
authors.initials: C
authors.lastname: Fu
authors.forename: Weiyuan
authors.initials: W
authors.lastname: Hou
authors.forename: Huanqin
authors.initials: H
authors.lastname: Dai
authors.forename: Xueting
authors.initials: X
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Xie
authors.forename: Ke
authors.initials: K
authors.lastname: Yu
authors.forename: Ruxian
authors.initials: R
authors.lastname: Chen
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000995:Antitubercular Agents / Q000378:metabolism* / Q000494:pharmacology; D001483:Base Sequence; D003470:Culture Media; D017931:DNA Primers; D003609:Dactinomycin / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D005285:Fermentation; D008386:Marine Biology; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
chemical_list: D000995:Antitubercular Agents; D003470:Culture Media; D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D003609:Dactinomycin; C041783:actinomycin X