title: Effect of negative pressure wound therapy on the elution of antibiotics from polymethylmethacrylate beads in a porcine simulated open femur fracture model.
abstract: OBJECTIVE
  To determine whether negative pressure wound therapy (NPWT) affects antibiotic elution in simulated femur fractures treated with antibiotic impregnated polymethylmethacrylate (PMMA) beads and whether fascial closure between beads and sponge affects the outcome.
  
  
  METHODS
  PMMA beads containing vancomycin and tobramycin were placed adjacent to bilateral corticotomies created in 20 anesthetized pigs. In 1 leg, NPWT was applied with the sponge either in direct contact with the beads or superficial to reapproximated fascia lata. The contralateral wound was conventionally closed. Vancomycin and tobramycin concentrations in wound drainage were measured every 12 hours for 72 hours, and tobramycin levels were measured in periosteal tissue obtained at 72 hours.
  
  
  RESULTS
  Drainage vancomycin and tobramycin concentrations were highest at 12 hours and fell rapidly by 24 hours but remained steady thereafter. At each 12-hour interval, there were no significant differences in the vancomycin and tobramycin concentrations between NPWT and control wound drainage, although whether the fascia was closed or left open had an influence on vancomycin levels. The total vancomycin and tobramycin eluted into the drains was significantly less in the NPWT group with open fascia. The antibiotic levels measured in wound drainage remained above the minimum inhibitory concentration for common wound organisms throughout the study period. Neither NPWT nor fascial closure had a significant effect on tobramycin periosteal tissue concentrations.
  
  
  CONCLUSIONS
  Concurrent application of NPWT with antibiotic impregnated PMMA beads to simulated open femur fractures in pigs did not decrease local antibiotic concentrations but did decrease the total amount of eluted vancomycin and tobramycin locally available when the fascia was left open.
authors.affiliation: Department of Orthopaedic, David Grant USAF Medical Center, Bodin Circle Travis Air Force Base, CA, USA. tom_large@hotmail.com
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Large
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Douglas
authors.forename: Gregory
authors.initials: G
authors.lastname: Erickson
authors.forename: J Kevin
authors.initials: JK
authors.lastname: Grayson
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry; D004195:Disease Models, Animal; D004304:Dosage Forms; D000071938:Fasciotomy; D005260:Female; D005264:Femoral Fractures / Q000188:drug therapy / Q000601:surgery / Q000628:therapy*; D005597:Fractures, Open / Q000188:drug therapy / Q000601:surgery / Q000628:therapy*; D008297:Male; D008863:Microspheres; D054843:Negative-Pressure Wound Therapy; D019904:Polymethyl Methacrylate; D013552:Swine; D014031:Tobramycin / Q000008:administration & dosage / Q000032:analysis; D016896:Treatment Outcome; D014640:Vancomycin / Q000008:administration & dosage / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D004304:Dosage Forms; D014640:Vancomycin; D019904:Polymethyl Methacrylate; D014031:Tobramycin