title: A simple, versatile method for GFP-based super-resolution microscopy via nanobodies.
abstract: We developed a method to use any GFP-tagged construct in single-molecule super-resolution microscopy. By targeting GFP with small, high-affinity antibodies coupled to organic dyes, we achieved nanometer spatial resolution and minimal linkage error when analyzing microtubules, living neurons and yeast cells. We show that in combination with libraries encoding GFP-tagged proteins, virtually any known protein can immediately be used in super-resolution microscopy and that simplified labeling schemes allow high-throughput super-resolution imaging.
authors.affiliation: Institute of Biochemistry, ETH Zurich, Switzerland.
authors.forename: Jonas
authors.initials: J
authors.lastname: Ries
authors.forename: Charlotte
authors.initials: C
authors.lastname: Kaplan
authors.forename: Evgenia
authors.initials: E
authors.lastname: Platonova
authors.forename: Hadi
authors.initials: H
authors.lastname: Eghlidi
authors.forename: Helge
authors.initials: H
authors.lastname: Ewers
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes; D058851:GPI-Linked Proteins / Q000276:immunology*; D049452:Green Fluorescent Proteins / Q000276:immunology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D036103:Nanotechnology; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D004718:Saccharomycetales / Q000648:ultrastructure
chemical_list: D005456:Fluorescent Dyes; D058851:GPI-Linked Proteins; D049452:Green Fluorescent Proteins