title: Biomarine extracts significantly protect from ultraviolet A-induced skin photoaging: an ex vivo study.
abstract: We tested the activity of the marine nutraceutical CL-1222 added with a coenzyme Q10 (CoQ10)-lutein-selenium component (Celergen(®), Laboratoires-Dom, Switzerland) to protect human fibroblasts against ultraviolet A (UVA)-induced photoaging. Cells obtained from 22- to 39-year-old healthy donors were pretreated with CL-1222 before UV irradiation, as compared with same quantity of the CoQ10-lutein-selenium component. As compared to untreated control, UVA-irradiated samples exhibited a significant increase of secreted matrix metalloproteinase-1 (MMP-1) (p<0.001) with over four-fold MMP-1 upregulation (p<0.001). Samples treated with CL-1222, but not with the CoQ10-lutein-selenium component, showed a significant decrease of MMP-1 secretion (p<0.01) and expression decrease (>60%, p<0.01) with >54% elastase activity inhibition (p<0.01). This preliminary study shows that such marine nutraceuticals can significantly protect against UV-irradiation irrespective of the CoQ10-lutein-selenium component with a specific protective gene expression modulation amenable to novel clinical applications.
authors.affiliation: ReGenera Res Group for Aging Intervention, Milan, Italy. fmarchimede@libero.it
authors.forename: Francesco
authors.initials: F
authors.lastname: Marotta
authors.forename: Archana
authors.initials: A
authors.lastname: Kumari
authors.forename: Hariom
authors.initials: H
authors.lastname: Yadav
authors.forename: Ascanio
authors.initials: A
authors.lastname: Polimeni
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Soresi
authors.forename: Aldo
authors.initials: A
authors.lastname: Lorenzetti
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Naito
authors.forename: Shalini
authors.initials: S
authors.lastname: Jain
mesh_terms: D000328:Adult; D001706:Biopsy / Q000379:methods; D003094:Collagen / Q000627:therapeutic use*; D004247:DNA / Q000627:therapeutic use*; D019587:Dietary Supplements; D004549:Elastin / Q000627:therapeutic use*; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D056604:Grape Seed Extract / Q000627:therapeutic use*; D006801:Humans; D014975:Lutein / Q000494:pharmacology; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D008954:Models, Biological; D010196:Pancreatic Elastase / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology; D015595:Skin Aging; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000494:pharmacology; D014466:Ultraviolet Rays; D015854:Up-Regulation
chemical_list: C574727:CL-1222; D056604:Grape Seed Extract; D014451:Ubiquinone; D003094:Collagen; D004247:DNA; D004549:Elastin; D010196:Pancreatic Elastase; D020781:Matrix Metalloproteinase 1; C024989:coenzyme Q10; D012643:Selenium; D014975:Lutein