title: A novel cost-effective medium for the production of Bacillus thuringiensis subsp. israelensis for mosquito control.
abstract: Bacillus thuringiensis subsp. israelensis (Bti) has been used for mosquito-control   programmes the world-wide. Indeed, the large-scale production of Bti for mosquito control is   very expensive due to the high cost of its culture. In the present study, we attempted to widen   the scope in developing cost-effective culture medium for Bti production, based on the raw   materials available on the biosphere, including coconut cake powder, CCP (Cocos nucifera),   neem cake powder, NCP (Azadirachta indica) and groundnut cake powder, GCP (Arachis   hypogea). Among these raw materials, the biomass production of Bti, sporulation and toxin   synthesizing from 'CCP' in combination with mineral salt (MnCl(2)) was comfortably satisfactory.   Bioassays with mosquito species (Culex quinquefasciatus, Anopheles stephensi and Aedes   aegypti) and field trials were also satisfactory. The present investigation suggests that coconut   cake-based culture medium can be used as an alternative for industrial production of Bti in   mosquito-control programme. Therefore, the study is very important from the point of effective   production of Bti from cost-effective culture medium for the control of mosquito vectors.
authors.affiliation: Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Ministry of Health and Family Welfare, Government of India, Indira Nagar, Pondicherry 60 5006, India. Subbiahpoopathi@rediffmail.com
authors.forename: Subbiah
authors.initials: S
authors.lastname: Poopathi
authors.forename: B
authors.initials: B
authors.lastname: Archana
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000254:growth & development*; D001427:Bacterial Toxins / Q000378:metabolism; D018533:Biomass; D003465:Culex / Q000382:microbiology / Q000502:physiology; D003470:Culture Media / Q000737:chemistry* / Q000191:economics*; D007814:Larva / Q000382:microbiology / Q000502:physiology; D009032:Mosquito Control / Q000379:methods*; D010572:Pest Control, Biological / Q000379:methods*; D013171:Spores, Bacterial / Q000254:growth & development; D016019:Survival Analysis
chemical_list: D001427:Bacterial Toxins; D003470:Culture Media