title: Growth and laboratory maintenance of Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa is a common, free-living, Gram-negative bacterium that can cause significant disease as an opportunistic pathogen. Rapid growth, facile genetics, and a large suite of virulence-related phenotypes make P. aeruginosa a common model organism to study Gram-negative opportunistic pathogens and basic microbiology. This unit describes the basic laboratory growth and maintenance of P. aeruginosa.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Vermont College of Medicine, Burlington, Vermont, USA.
authors.forename: Annette E
authors.initials: AE
authors.lastname: LaBauve
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Wargo
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D011309:Preservation, Biological; D011550:Pseudomonas aeruginosa / Q000254:growth & development*