title: Molecular analysis of mucopolysaccharidosis IVA (Morquio A) in Spain.
abstract: Mucopolysaccharidosis type IVA (Morquio A) is an inherited metabolic disease with autosomal recessive inheritance. The pathology is due to a deficient activity of N-acetylgalactosamine-6-sulfate-sulfatase, which is involved in the degradation of keratan sulfate and chondroitin-6-sulfate. To date more than 150 mutations have been described in the GALNS gene in different populations. The aim of this study was to analyze the mutations and polymorphisms in Spain in order to know the epidemiology of our population and also to offer genetic counseling to affected families. We found 30 mutant alleles in the 15 families analyzed completing all the genotypes. Most of the mutations that we found were missense mutations, six of which were novel: p.S74F, p.E121D, p.Y254C, p.E260K, p.T394P and p.N495Y; we also found a small deletion (c.1142delC) and a probable deep intronic mutation that causes the loss of exon 5 (c.423_566del) found in cDNA. Both mutations are described in this study for the first time. We also identified 20 polymorphisms previously reported and 2 novel ones: (c.633+222T/C and c.898+25C>G). In conclusion, we have identified the mutations responsible for Mucopolysaccharidosis IV A in Spain. We found great allelic heterogeneity, as occurs in other populations, which hinders the establishment of genotype-phenotype correlations in Spain. This study has been very useful for genetic counseling to the affected families.
authors.affiliation: Sección de Errores Congénitos del Metabolismo-IBC, Servicio de Bioquímica y Genética Molecular, Hospital Clínic, CIBERER, IDIBAPS, Barcelona, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Pajares
authors.forename: Carlos
authors.initials: C
authors.lastname: Alcalde
authors.forename: Ma Luz
authors.initials: ML
authors.lastname: Couce
authors.forename: Mireia
authors.initials: M
authors.lastname: Del Toro
authors.forename: Antonio
authors.initials: A
authors.lastname: González-Meneses
authors.forename: Encarna
authors.initials: E
authors.lastname: Guillén
authors.forename: Mercè
authors.initials: M
authors.lastname: Pineda
authors.forename: Guillem
authors.initials: G
authors.lastname: Pintos
authors.forename: Laura
authors.initials: L
authors.lastname: Gort
authors.forename: Ma José
authors.initials: MJ
authors.lastname: Coll
mesh_terms: D000293:Adolescent; D000328:Adult; D017668:Age of Onset; D000483:Alleles; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D002811:Chondroitinsulfatases / Q000235:genetics*; D005190:Family; D005260:Female; D023061:Gene Order; D006239:Haplotypes; D006801:Humans; D007223:Infant; D008297:Male; D009085:Mucopolysaccharidosis IV / Q000453:epidemiology / Q000235:genetics*; D059645:Mutation Rate; D020641:Polymorphism, Single Nucleotide; D013030:Spain / Q000453:epidemiology; D055815:Young Adult
chemical_list: D002811:Chondroitinsulfatases; C102295:GALNS protein, human