title: mBin1b transgenic mice show enhanced resistance to epididymal infection by bacteria challenge.
abstract: The mBin1b is a beta-defensin gene identified in the mouse epididymis. In the current report, its expression pattern and antibacterial activities were characterized, and a transgenic (TG) mouse model was developed in which mBin1b was exclusively overexpressed by up to 50-fold over normal levels in the caput epididymis. The experimental animals are healthy with normal reproductive activity, but are more resistant to epididymal infection from Escherichia coli than normal animals. The expression of IL1α and IL1β in the epididymis was decreased in the TG mice, which suggests that mBin1b has a role in the regulation of inflammatory response in the epididymis.
authors.affiliation: Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory for Molecular Biology, Institute of Biochemistry and Cell biology, Shanghai, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Fei
authors.forename: S
authors.initials: S
authors.lastname: Hu
authors.forename: L
authors.initials: L
authors.lastname: Xiao
authors.forename: J
authors.initials: J
authors.lastname: Zhou
authors.forename: H
authors.initials: H
authors.lastname: Diao
authors.forename: H
authors.initials: H
authors.lastname: Yu
authors.forename: S
authors.initials: S
authors.lastname: Fang
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Wan
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Xu
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Fei
mesh_terms: D000818:Animals; D001483:Base Sequence; D004195:Disease Models, Animal; D004822:Epididymis / Q000378:metabolism; D004823:Epididymitis / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D004927:Escherichia coli Infections / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution; D015854:Up-Regulation; D023083:beta-Defensins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C465595:Spag11 protein, mouse; D023083:beta-Defensins