title: The role of N-acetylglucosaminyltransferases V in the malignancy of human hepatocellular carcinoma.
abstract: To investigate the role of N-acetylglucosaminyltransferases V (GnT-V) in the malignancy of human hepatocellular carcinoma (HCC), the GnT-V stably suppressed cell line HepG2 GnT-V/1564 was constructed from HepG2. The proliferation, migration, invasion, metastasis of HepG2 GnT-V/1564 was investigated both in vitro and in vivo. The clinical pathological significance of GnT-V expression was also studied in 140 cases of HCC tissues. This study showed that down-regulation of GnT-V inhibited the proliferation, migration and invasion of the HepG2 cells. In addition, GnT-V expression was shown in 138 cases of 140 (98.6%) HCC samples, in 3 cases of 31 (9.7%) in liver cirrhosis cases and in 1 cases of 20 (5.0%) in normal liver tissues. Besides, a higher level of GnT-V was observed more frequently in the advanced tumors with higher T stage and histological grade. These data suggested that GnT-V expression was positively related with malignancy in HCC and GnT-V may be both a differentiation marker and a potential target for the treatment of HCC.
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wei
authors.forename: Qiulian
authors.initials: Q
authors.lastname: Liu
authors.forename: Fuli
authors.initials: F
authors.lastname: He
authors.forename: Weiliang
authors.initials: W
authors.lastname: Zhu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Hu
authors.forename: Linlang
authors.initials: L
authors.lastname: Guo
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000201:enzymology; D008103:Liver Cirrhosis / Q000201:enzymology; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D017351:N-Acetylglucosaminyltransferases / Q000096:biosynthesis*; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D023041:Xenograft Model Antitumor Assays
chemical_list: D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase