title: [Insulinotropic action of hippocampal cholinergic neurostimulating peptide mediated by activated type 3 muscarinic receptor in INS-1 cells].
abstract: OBJECTIVE
  [corrected] To characterize the insulinotropic action of hippocampal cholinergic neurostimulating peptide (HCNP) and analyze the role of type 3 muscarinic receptor (M(3)R) pathway in the action of HCNP.
  
  
  METHODS
  INS-1 cells were incubated in routine RPMI 1640 medium (control group), RPMI 1640 supplemented with 50 pg/ml synthetic HCNP (HCNP group), or HCNP-containing medium with the addition of PMA 18 h prior to insulin release assay. The insulin levels in the medium was measured using radioimmunoassay following stimulation with different concentrations of glucose. Real-time quantitative PCR was used for detecting the gene expression of HCNP-pp, choline acetyltransferase (ChAT) and M(3)R in HCNP group and control group.
  
  
  RESULTS
  After stimulation with different concentrations of glucose (5.6 and 16.7 mmol/L), HCNP group showed significantly higher insulin levels than the control and HCNP+ PMA groups. Compared with those in the control group, the mRNA levels of HCNP-pp, ChAT, and M(3)R were all lowered in HCNP group.
  
  
  CONCLUSIONS
  HCNP can promote insulin release in INS-1 cells by increasing ChAT activity and activating M(3)R, and this effect is inhibited by PMA.
authors.affiliation: Department of Metabolism and Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. gaofokok@126.com
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Nanjing
authors.initials: N
authors.lastname: Guo
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Xu
authors.forename: Dehong
authors.initials: D
authors.lastname: Cai
mesh_terms: D000818:Animals; D002460:Cell Line; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D009479:Neuropeptides / Q000494:pharmacology*; D051381:Rats; D043587:Receptor, Muscarinic M3 / Q000378:metabolism*
chemical_list: D007328:Insulin; D009479:Neuropeptides; D043587:Receptor, Muscarinic M3; C075274:hippocampal cholinergic neurostimulating peptide