title: Human embryonic stem cells derived in xeno-free conditions.
abstract: In this chapter, we describe the derivation and characterization of nine hIn this chapter, we describe the derivation and characterization of nine human embryonic stem cells (hESC) (VAL-3 to -11B) from different developmental embryo stages (inner cell mass from a blastocyst, morula, and blastomere from a 3-day embryo) under xeno-free conditions providing the necessary protocols and techniques to carry out their derivation, characterization, and propagation.
authors.affiliation: Valencia Node of the National Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: Galán
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
mesh_terms: D046149:Embryo Culture Techniques / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping