title: [TNFα induced IL-8 production through p38 MAPK- NF-kB pathway in human hepatocellular carcinoma cells].
abstract: OBJECTIVE
  To identify the role of p38 MAPK- NF-kB signaling pathway in TNF-α induced IL-8 production in human hepatocellular carcinoma cells.
  
  
  METHODS
  The concentrations of IL-8 from MHCC-97H cells were measured by an enzyme-linked immunosorbent assay (ELISA). The phosphorylation of p38 MAPK was analyzed by Western blot and immunofluorescence. NF-kB p65 protein nuclear translocation was determined by non-radioactive NF-kB p50 / p65 transcription factor activity kit and immunofluorescence.
  
  
  RESULTS
  The IL-8 production from MHCC-97H cells challenged with TNFa significantly increased in a time-dependent (F = 144.04, P < 0.01) and dose-dependent (F = 364.14, P < 0.01) manners, as compared with those without TNFa challenge. TNFa up-regulated the phosphorylation levels of p38 MAPK and increased the translocation of NF-kB p65 protein into the nucleus, also proved by immunofluorescence staining. p38 MAPK inhibitor (SB203580) could significantly inhibit IL-8 production in a dose-dependent manners (F = 65.47, P < 0.01), and partially inhibited NF-kB p65 nuclear translocation in a dose-dependent manner (F=141.20, P < 0.05).
  
  
  CONCLUSIONS
  TNF-α could increase the production of IL-8 in MHCC-97H cells and p38 MAPK- NF-kB pathways seem to play a central role in the regulation of IL-8 production.
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yao-hui
authors.initials: YH
authors.lastname: Wang
authors.forename: Jing-lin
authors.initials: JL
authors.lastname: Xia
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Wang
authors.forename: Bi-wei
authors.initials: BW
authors.lastname: Yang
authors.forename: Jie-feng
authors.initials: JF
authors.lastname: Cui
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Fan
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D016209:Interleukin-8; C498143:RELA protein, human; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D048051:p38 Mitogen-Activated Protein Kinases