title: β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: role of prostaglandin E(2).
abstract: In this paper, we demonstrate that circulating antibodies from chronic periodontitis patients reacting with atrial β(1)-adrenoceptors (β(1)-ARs) act as an inducer of soluble CD40 ligand (sCD40L) release and prostaglandin E(2) (PGE(2)) generation. By enzyme-linked immunosorbent assay using β(1) synthetic peptide (with an amino acid sequence identical to the second loop of human myocardial β(1)-ARs) as a coating antigen, we demonstrated reactivity against the second extracellular loop on human myocardial β(1)-ARs. This autoantibody present in the serum of chronic periodontitis patients was significantly correlated with the release of sCD40L and PGE(2). The release of sCD40L was blunted by atenolol, SP600125 and β(1) synthetic peptide, and PGE(2) generation was inhibited by DuP 697 and slightly by FR122049. The effects of the antibody incubated with isolated rat atria upregulated sCD40L release with an increase of PGE(2) production and c-Jun N-terminal kinase phosphorylation. These results indicate that in chronic periodontitis patients, there is a positive association between sCD40L release and PGE(2) generation via the action of β(1)-AR antibodies.
authors.affiliation: Pharmacology Unit, School of Dentistry, University of Buenos Aires, Argentina.
authors.forename: Leonor
authors.initials: L
authors.lastname: Sterin-Borda
authors.forename: Marcela
authors.initials: M
authors.lastname: Segovia
authors.forename: Silvia
authors.initials: S
authors.lastname: Reina
authors.forename: Enri
authors.initials: E
authors.lastname: Borda
mesh_terms: D000328:Adult; D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D023201:CD40 Ligand / Q000097:blood / Q000276:immunology*; D055113:Chronic Periodontitis / Q000097:blood / Q000276:immunology*; D015232:Dinoprostone / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology / Q000302:isolation & purification; D048031:JNK Mitogen-Activated Protein Kinases / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D018342:Receptors, Adrenergic, beta-1 / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D018342:Receptors, Adrenergic, beta-1; D023201:CD40 Ligand; D048031:JNK Mitogen-Activated Protein Kinases; D015232:Dinoprostone