title: Multicenter uric acid biosensor based on tetrapod-shaped ZnO nanostructures.
abstract: In this work, we developed a tetrapod-shaped ZnO nanostructure (T-ZnO) biosensor to determine uric acid (UA), which is the primary end product of purine metabolism. The as-fabricated UA sensor presents a higher performance than that of the reported biosensors based on ZnO nanorods and ZnS quantum dots, etc. High-quality ZnO nanotetrapods were characterized by field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectra (EDX), X-ray diffraction (XRD) and Raman spectroscopy, respectively. A high affinity of uricase/ZnO to UA was revealed by cyclic voltammograms. The biosensor performance has been systematically investigated by amperometric response measurements. A fast current response time is within 9 s. It was also found that the uricase/T-ZnO biosensor presented a high and reproducible sensitivity of 80.0 microA cm(-2) mM(-1) and an experiment limit of detection of 0.8 microM. This study provides an insight utilizing the unique ZnO nanostructure to develop the highly sensitive and rapidly responsive nano-bio devices.
authors.affiliation: State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lei
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Yan
authors.forename: Yu
authors.initials: Y
authors.lastname: Song
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Kang
authors.forename: Ning
authors.initials: N
authors.lastname: Luo
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D014503:Urate Oxidase / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D014503:Urate Oxidase; D015034:Zinc Oxide