title: [Effect of RNA interference on small heat shock protein Sjp40 of Schistosoma japonicum].
abstract: OBJECTIVE
  To study the effect of RNA interference (RNAi) on small heat shock protein (sHSP) Sjp40 of Schistosoma japonicum and its synergistic effect on the expression of SjHSP60, SjHSP70, and SjHSP90 mRNA, and observe the mRNA expression levels of Sjp40, SjHSP60, SjHSP70, and SjHSP90 in different stages of S.japonicum.
  
  
  METHODS
  Double-stranded RNA (dsRNA) of Sjp40 (dsSjp40) and a control dsRNA of green fluorescent protein (dsGFP) were generated by in vitro transcription and transfected into adult worm by immersing the worm in dsRNA solution. The total RNA and proteins were isolated simultaneously from the adult worms using TRIzol reagent 7 days after transfection. The expression levels of Sjp40, SjHSP60, SjHSP70, and SjHSP90 mRNA and the expression level of Sjp40 protein were determined by quantitative real-time PCR (qPCR) and Western blotting, respectively. The mRNA expression of HSPs of S. japonicum in different stages was evaluated by qPCR.
  
  
  RESULTS
  Compared with those in the control worms transfected with dsGFP, Sjp40 mRNA level was decreased by 80% in the worms transfected with dsSjp40, and the level of Sjp40 protein showed also a significant decrease. The mRNA expression levels of SjHSP60, SjHSP70, and SjHSP90 did not show an obvious synergism after Sjp40 RNAi. The expression profiles of Sjp40, SjHSP60, SjHSP70, and SjHSP90 showed significant differences in different stages of S. japonicum, and the expression level of Sjp40 mRNA in the egg stage was much higher than that of other HSP genes.
  
  
  CONCLUSIONS
  dsSjp40-RNAi can induce effective suppression of Sjp40 gene expression at both mRNA and protein levels, but no obvious synergism occurs in the mRNA expressions of SjHSP60, SjHSP70, and SjHSP90.
authors.affiliation: Department of Parasitology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. minmin2736@163.com
authors.forename: Minfang
authors.initials: M
authors.lastname: Chen
authors.forename: Rongjia
authors.initials: R
authors.lastname: Mai
authors.forename: Qianzhen
authors.initials: Q
authors.lastname: Mo
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D050888:Heat-Shock Proteins, Small / Q000235:genetics*; D015801:Helminth Proteins / Q000235:genetics*; D034622:RNA Interference; D012330:RNA, Double-Stranded; D012333:RNA, Messenger / Q000235:genetics; D012549:Schistosoma japonicum / Q000235:genetics*
chemical_list: D050888:Heat-Shock Proteins, Small; D015801:Helminth Proteins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger