title: Dengue virus identification by transmission electron microscopy and molecular methods in fatal dengue hemorrhagic fever.
abstract: Dengue virus is the most significant virus transmitted by arthropods worldwide and may cause a potentially fatal systemic disease named dengue hemorrhagic fever. In this work, dengue virus serotype 4 was detected in the tissues of one fatal dengue hemorrhagic fever case using electron immunomicroscopy and molecular methods. This is the first report of dengue virus polypeptides findings by electron immunomicroscopy in human samples. In addition, not-previously-documented virus-like particles visualized in spleen, hepatic, brain, and pulmonary tissues from a dengue case are discussed.
authors.affiliation: Arbovirus Laboratory, Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute (IPK), P.O. Box 601, Marianao 13, Havana, Cuba.
authors.forename: D
authors.initials: D
authors.lastname: Limonta
authors.forename: V
authors.initials: V
authors.lastname: Falcón
authors.forename: G
authors.initials: G
authors.lastname: Torres
authors.forename: V
authors.initials: V
authors.lastname: Capó
authors.forename: I
authors.initials: I
authors.lastname: Menéndez
authors.forename: D
authors.initials: D
authors.lastname: Rosario
authors.forename: Y
authors.initials: Y
authors.lastname: Castellanos
authors.forename: M
authors.initials: M
authors.lastname: Alvarez
authors.forename: R
authors.initials: R
authors.lastname: Rodríguez-Roche
authors.forename: M C
authors.initials: MC
authors.lastname: de la Rosa
authors.forename: A
authors.initials: A
authors.lastname: Pavón
authors.forename: L
authors.initials: L
authors.lastname: López
authors.forename: K
authors.initials: K
authors.lastname: González
authors.forename: G
authors.initials: G
authors.lastname: Guillén
authors.forename: J
authors.initials: J
authors.lastname: Diaz
authors.forename: M G
authors.initials: MG
authors.lastname: Guzmán
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D001921:Brain / Q000648:ultrastructure / Q000821:virology; D003462:Cuba; D004279:DNA, Viral / Q000032:analysis; D003716:Dengue Virus / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure*; D004797:Enzyme-Linked Immunosorbent Assay; D017809:Fatal Outcome; D005260:Female; D006321:Heart / Q000821:virology; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007668:Kidney / Q000648:ultrastructure / Q000821:virology; D008099:Liver / Q000648:ultrastructure / Q000821:virology; D046529:Microscopy, Electron, Transmission / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019595:Severe Dengue / Q000175:diagnosis* / Q000821:virology; D013154:Spleen / Q000648:ultrastructure / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007075:Immunoglobulin M