title: Glioma immunotherapy with combined autologous tumor cell and endothelial cell vaccine in vivo.
abstract: Combined vaccines containing GL261 murine glioma cells and F-2 murine endothelial cells fixed with glutaraldehyde-phosphate buffered saline were injected into the intradermal tissue of the tail base of C57BL/6 mice. After the vaccination, GL261 cells were injected subcutaneously into the left flank of the mice. Vaccination with fixed F-2 cells induced the development of relatively high amounts of interferon-gamma-releasing cells after in vitro re-stimulation with vascular endothelial growth factor-receptor 2 peptide. Tumor growth was inhibited after preventive use of the combined vaccine, prepared from GL261 and F-2 cells. Tumor specimens obtained from the combined vaccine group in a therapeutic experiment showed significantly decreased vessel count. Glioma immunotherapy with a combined vaccine prepared from tumor cells and endothelial cells might represent a new clinical strategy, as such combinations may theoretically affect both high-grade glioma cells and their environment.
authors.affiliation: Department of Neurosurgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Youji
authors.initials: Y
authors.lastname: Uemae
authors.forename: Eiichi
authors.initials: E
authors.lastname: Ishikawa
authors.forename: Shingo
authors.initials: S
authors.lastname: Takano
authors.forename: Kei
authors.initials: K
authors.lastname: Nakai
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Alexander
authors.initials: A
authors.lastname: Zaboronok
authors.forename: Akira
authors.initials: A
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000098:blood supply / Q000276:immunology / Q000628:therapy*; D019496:Cancer Vaccines / Q000276:immunology / Q000494:pharmacology*; D045744:Cell Line, Tumor; D017690:Cell Transplantation / Q000379:methods*; D005910:Glioma / Q000098:blood supply / Q000276:immunology / Q000628:therapy*; D007167:Immunotherapy / Q000379:methods; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation / Q000379:methods; D009374:Neoplasms, Experimental / Q000276:immunology / Q000503:physiopathology / Q000628:therapy*; D061251:Primary Cell Culture; D017778:Vaccines, Combined / Q000276:immunology / Q000494:pharmacology*
chemical_list: D019496:Cancer Vaccines; D017778:Vaccines, Combined