title: RmtF, a new member of the aminoglycoside resistance 16S rRNA N7 G1405 methyltransferase family.
abstract: Multidrug-resistant clinical isolate Klebsiella pneumoniae BM4686 was highly resistant to 4,6-disubstituted 2-deoxystreptamines and to fortimicin. Resistance was due to the presence, on the 40-kb non-self-transferable plasmid pIP849, of the rmtF gene which was cotranscribed with the upstream aac(6')-Ib gene. The deduced RmtF protein had 25 to 46% identity with members of the N7 G1405 family of aminoglycoside resistance 16S rRNA methyltransferases.
authors.affiliation: Institut Pasteur, Unité des Agents Antibactériens, Paris, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Galimand
authors.forename: Patrice
authors.initials: P
authors.lastname: Courvalin
authors.forename: Thierry
authors.initials: T
authors.lastname: Lambert
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000378:metabolism*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; C023521:fortimicins; D008780:Methyltransferases