title: Functional characterizations of malonyl-CoA:acyl carrier protein transacylase (MCAT) in Eimeria tenella.
abstract: Eimeria tenella, an apicomplexan parasite in chickens, possesses an apicoplast and its associated metabolic pathways including the Type II fatty acid synthesis (FAS II). Malonyl-CoA:acyl-carry protein transacylase (MCAT) encoded by the fabD gene is one of the essential enzymes in the FAS II system. In the present study, the entire E. tenella MCAT gene (EtfabD) was cloned and sequenced. Immunolabeling located this protein in the apicoplast organelle in coccidial sporozoites. Functional replacement of the fabD gene with amber mutation of E. coli temperature-sensitive LA2-89 strain by E. tenella EtMCAT demonstrated that EcFabD and EtMCAT perform the same biochemical function. The recombinant EtMCAT protein was expressed and its general biochemical features were also determined. An alkaloid natural product corytuberine (CAS: 517-56-6) could specifically inhibit the EtMCAT activity (IC(50)=16.47μM), but the inhibition of parasite growth in vitro by corytuberine was very weak (the predicted MIC(50)=0.65mM).
authors.affiliation: Institute of Veterinary Medicine, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
authors.forename: Mingfei
authors.initials: M
authors.lastname: Sun
authors.forename: Guan
authors.initials: G
authors.lastname: Zhu
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Qin
authors.forename: Caiyan
authors.initials: C
authors.lastname: Wu
authors.forename: Minna
authors.initials: M
authors.lastname: Lv
authors.forename: Shenquan
authors.initials: S
authors.lastname: Liao
authors.forename: Nanshan
authors.initials: N
authors.lastname: Qi
authors.forename: Mingquan
authors.initials: M
authors.lastname: Xie
authors.forename: Jianping
authors.initials: J
authors.lastname: Cai
mesh_terms: D051080:Acyl-Carrier Protein S-Malonyltransferase / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D016786:Eimeria tenella / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D005816:Genetic Complementation Test; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D015388:Organelles / Q000737:chemistry; D015800:Protozoan Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D016054:DNA, Protozoan; D005227:Fatty Acids; D015800:Protozoan Proteins; D051080:Acyl-Carrier Protein S-Malonyltransferase