title: Characterization of surface properties and cytocompatibility of ion-etched chitosan films.
abstract: Surface modification of biomaterials has been highlighted by biomedical engineers as a facile method for improving cell-biomaterial interactions without the expense and time required to develop new materials. In the present study, we investigated the influence of ion-etching on the surface characteristics of chitosan films using XPS and ATR FT-IR. The physiological behavior of human dermal fibroblasts (hDFs) grown on such surfaces was studied by evaluating adhesive and proliferative properties, and by examining surface morphologies of hDFs using AFM. hDFs displayed different shapes depending on the ion-etching time. hDFs grown on chitosan films ion-etched for 5 min displayed better development of lamellipodia and filopodia around the hDF periphery than did cells grown on nonmodified chitosan film, whereas hDFs did not spread well on films ion-etched for 20 min. Films ion-etched for 5 min or less had higher NH(2) and COOH contents, leading to enhanced hDF adhesion and proliferation.
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, 75 Nowon-gil, Nowon-gu, Seoul, 139-706, Korea.
authors.forename: Young Gun
authors.initials: YG
authors.lastname: Ko
authors.forename: Seong Mi
authors.initials: SM
authors.lastname: Yu
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Park
authors.forename: Heung Jae
authors.initials: HJ
authors.lastname: Chun
authors.forename: Chun-Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007477:Ions / Q000737:chemistry; D008567:Membranes, Artificial; D012867:Skin / Q000166:cytology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D007477:Ions; D008567:Membranes, Artificial; D048271:Chitosan