title: Bioenergetics for the growth of Staphylococcus lentus in biocompatible choline salts.
abstract: Choline-based biocompatible salts were used as "nutrients" for the growth of Staphylococcus lentus bacteria. Increase in the growth rate of bacteria was observed, compared to conventional carbon sources. In the case of the ionic liquid, choline lactate, the increase was pronounced. Bacterial growth was correlated with power-time curve in an investigation monitored online by reaction calorimetry. From the power-time curve, three phases of the growth can be distinctly seen. Heat yield coefficients estimated for the growth of S. lentus were found to match well with those reported hitherto. A comparative study of heat yields (catabolic) between glucose and choline lactate revealed significant information; the heat yield due to choline lactate (Y                 (Q/S)) consumption and oxygen (Y                 (Q/O)) were 23.4 kJ/g and 435 kJ/mol and whereas that for glucose with oxygen were 9.6 kJ/g and 427 kJ/mol, respectively, showing clearly the preferential affinity of choline lactate by the bacteria rather than glucose. This study also established that the use of ionic liquids as nutrients can be monitored using bioreaction calorimetry.
authors.affiliation: Thermochemical Lab, Chemical Engineering Department, Central Leather Research Institute-CLRI, Adyar, Chennai 600020 Tamil Nadu, India.
authors.forename: Sudharshan
authors.initials: S
authors.lastname: Sekar
authors.forename: Surianarayanan
authors.initials: S
authors.lastname: Mahadevan
authors.forename: Ranganathan
authors.initials: R
authors.lastname: Vijayaraghavan
authors.forename: Asit Baran
authors.initials: AB
authors.lastname: Mandal
authors.forename: D R
authors.initials: DR
authors.lastname: MacFarlane
mesh_terms: D002794:Choline / Q000378:metabolism*; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D013210:Staphylococcus / Q000737:chemistry / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D005947:Glucose; D002794:Choline