title: Antifungal activities of different extracts of marine macroalgae against dermatophytes and Candida species.
abstract: Algae are bioactive natural resources, and due to the medical importance of superficial mycoses, we focused the action of macroalgae extracts against dermatophytes and Candida species. Seaweed obtained from the Riacho Doce beach, Alagoas (Brazil), were screened for the antifungal activity, through crude extracts using dichloromethane, chloroform, methanol, ethanol, water and chloroform and hexane fractions of green, brown and red algae in assays with standard strains of the dermatophytes Trichophyton rubrum, T. tonsurans, T. mentagrophytes, Microsporum canis, M. gypseum and yeasts Candida albicans, C. krusei, C. guilliermondi and C. parapsilosis. The M44-A and M27-A2/M38A manuals by CLSI were followed, and the minimum inhibitory concentration (MIC) ranged from 0.03 to 16.00 μg ml(-1), and an inhibition halo of 10.00-25.00 mm was observed for dermatophytes, while for yeast, it was from 8.00 to 16.00 μg ml(-1) and 10.00-15.00 mm. M. canis showed MIC of 0.03 μg ml(-1) and the largest inhibition halo in T. rubrum (25.00 mm) through the use of the methanol extract. For C. albicans, dichloromethane, methanol and ethanol extracts formed the largest inhibition halo. The ethanol extract was shown to be the best inhibiting fungi growth, and chloroform and hexane fractions of H. musciformis inhibited the growth of all dermatophytes and C. albicans, yielding the conclusion that apolar extracts obtained from algae presented the best activity against important pathogenic fungi.
authors.affiliation: Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió, Alagoas, Brazil. eac.guedes@gmail.com
authors.forename: Elica Amara Cecilia
authors.initials: EA
authors.lastname: Guedes
authors.forename: Maria Anilda dos Santos
authors.initials: MA
authors.lastname: Araújo
authors.forename: Aryanna Kelly Pinheiro
authors.initials: AK
authors.lastname: Souza
authors.forename: Larissa Isabela Oliveira
authors.initials: LI
authors.lastname: de Souza
authors.forename: Lurdiana Dayse
authors.initials: LD
authors.lastname: de Barros
authors.forename: Fernanda Cristina de Albuquerque
authors.initials: FC
authors.lastname: Maranhão
authors.forename: Antônio Euzébio Goulart
authors.initials: AE
authors.lastname: Sant'Ana
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects*; D001938:Brazil; D002175:Candida / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D012624:Seaweed / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents