title: Nanoliter hemolymph sampling and analysis of individual adult Drosophila melanogaster.
abstract: The fruit fly (Drosophila melanogaster) is an extensively used and powerful, genetic model organism. However, chemical studies using individual flies have been limited by the animal's small size. Introduced here is a method to sample nanoliter hemolymph volumes from individual adult fruit-flies for chemical analysis. The technique results in an ability to distinguish hemolymph chemical variations with developmental stage, fly sex, and sampling conditions. Also presented is the means for two-point monitoring of hemolymph composition for individual flies.
authors.affiliation: Department of Chemistry MC 111, University of Illinois at Chicago, Chicago, Illinois 60607, United States.
authors.forename: Sujeewa C
authors.initials: SC
authors.lastname: Piyankarage
authors.forename: David E
authors.initials: DE
authors.lastname: Featherstone
authors.forename: Scott A
authors.initials: SA
authors.lastname: Shippy
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D004331:Drosophila melanogaster / Q000254:growth & development / Q000378:metabolism; D019075:Electrophoresis, Capillary; D005260:Female; D006458:Hemolymph / Q000737:chemistry*; D007814:Larva / Q000737:chemistry; D008297:Male; D036103:Nanotechnology; D012737:Sex Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000596:Amino Acids