title: Comparative transcriptome analysis of transporters, phytohormone and lipid metabolism pathways in response to arsenic stress in rice (Oryza sativa).
abstract: • Arsenic (As) contamination of rice (Oryza sativa) is a worldwide concern and elucidating the molecular mechanisms of As accumulation in rice may provide promising solutions to the problem. Previous studies using microarray techniques to investigate transcriptional regulation of plant responses to As stress have identified numerous differentially expressed genes. However, little is known about the metabolic and regulatory network remodelings, or their interactions with microRNA (miRNA) in plants upon As(III) exposure. • We used Illumina sequencing to acquire global transcriptome alterations and miRNA regulation in rice under As(III) treatments of varying lengths of time and dosages. • We found that the response of roots was more distinct when the dosage was varied, whereas that of shoots was more distinct when the treatment time was varied. In particular, the genes involved in heavy metal transportation, jasmonate (JA) biosynthesis and signaling, and lipid metabolism were closely related to responses of rice under As(III) stress. Furthermore, we discovered 36 new As(III)-responsive miRNAs, 14 of which were likely involved in regulating gene expression in transportation, signaling, and metabolism. • Our findings highlight the significance of JA signaling and lipid metabolism in response to As(III) stress and their regulation by miRNA, which provides a foundation for subsequent functional research.
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Lu-jun
authors.initials: LJ
authors.lastname: Yu
authors.forename: Ying-feng
authors.initials: YF
authors.lastname: Luo
authors.forename: Bin
authors.initials: B
authors.lastname: Liao
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Xie
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Shi
authors.initials: S
authors.lastname: Xiao
authors.forename: Jin-tian
authors.initials: JT
authors.lastname: Li
authors.forename: Song-nian
authors.initials: SN
authors.lastname: Hu
authors.forename: Wen-sheng
authors.initials: WS
authors.lastname: Shu
mesh_terms: D001151:Arsenic / Q000633:toxicity*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects*; D019216:Metals, Heavy / Q000493:pharmacokinetics; D035683:MicroRNAs; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D002352:Carrier Proteins; D003517:Cyclopentanes; D019216:Metals, Heavy; D035683:MicroRNAs; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; C011006:jasmonic acid; D001151:Arsenic