title: Analysis of polymorphisms in T(H)2-associated genes in Russian patients with atopic bronchial asthma.
abstract: BACKGROUND
  Bronchial asthma is a chronic respiratory disorder characterized by airway inflammation, airway hyperresponsiveness, and periodic reversible airway obstruction. Subtype 2 helperT cell (T(H)2) cytokines play an important role in the development of allergic airway inflammation in patients with bronchial asthma.
  
  
  OBJECTIVE
  To investigate whether the single-nucleotide polymorphisms (SNPs) Ile75Val and Gln576Arg in the IL4RA gene, -33C>T in the IL4 gene, and Gly237Glu in the FCER1B gene contribute to the development and severity of atopic bronchial asthma in Russian patients from Moscow.
  
  
  METHODS
  We analyzed DNA samples from 224 patients with atopic bronchial asthma and 172 healthy individuals. Genotyping was performed by primer extension followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
  
  
  RESULTS
  We observed a moderate association between the Arg/Arg genotype of Gln576Arg and protection against asthma (odds ratio [OR], 0.16; P < .012) and a strong association between the T allele and TT genotype of -33C> and atopic bronchial asthma (OR, 1.91 and 4.65, respectively; P < .0001). Carriers of the C allele had a reduced risk of asthma (OR, 0.53; P < .0001). Furthermore, we found that the TT genotype of -33C>T correlated with higher concentrations of total serum immunoglobulin E and interleukin 4 than the CC and CT genotypes.
  
  
  CONCLUSIONS
  We found an association between atopic bronchial asthma and the SNPs Gln576Arg in IL4RA and -33C>T in IL4. IL4RA and IL4 seem to be involved in the pathogenesis of asthma.
authors.affiliation: Laboratory of Chemical Genomics, Department for Proteomic Research, Research Institute of Biomedical Chemistry RAMS, Moscow, Russian Federation.
authors.forename: E V
authors.initials: EV
authors.lastname: Dmitrieva-Zdorova
authors.forename: O E
authors.initials: OE
authors.lastname: Voronko
authors.forename: E A
authors.initials: EA
authors.lastname: Latysheva
authors.forename: G I
authors.initials: GI
authors.lastname: Storozhakov
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D000328:Adult; D000402:Airway Obstruction / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000483:Alleles; D001249:Asthma / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics; D007249:Inflammation / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009031:Moscow; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D017455:Receptors, IgE / Q000235:genetics*; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D044465:White People / Q000235:genetics
chemical_list: C502447:MS4A2 protein, human; D017455:Receptors, IgE; D015847:Interleukin-4; D007073:Immunoglobulin E