title: Dynamic spectroscopic phase microscopy for quantifying hemoglobin concentration and dynamic membrane fluctuation in red blood cells.
abstract: We report a technique for simultaneous label-free quantification of cytoplasmic hemoglobin Hb concentration and dynamic membrane fluctuation in individual red blood cells (RBCs). Spectroscopic phase microscopy equipped with three different coherent laser sources and a color detector records three wavelength-dependent quantitative phase images in a single shot of a color-coded hologram. Using molecular specific dispersion, we demonstrate the extraction of Hb concentration and the dynamic membrane fluctuation from individual RBCs.
authors.affiliation: Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon, 305-701 South Korea.
authors.forename: Yunhun
authors.initials: Y
authors.lastname: Jang
authors.forename: Jaeduck
authors.initials: J
authors.lastname: Jang
authors.forename: YongKeun
authors.initials: Y
authors.lastname: Park
mesh_terms: D002478:Cells, Cultured; D003124:Colorimetry / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004910:Erythrocyte Membrane / Q000502:physiology* / Q000648:ultrastructure*; D006454:Hemoglobins / Q000032:analysis*; D006696:Holography / Q000295:instrumentation; D006801:Humans; D008560:Membrane Fluidity; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation*; D057054:Molecular Imaging / Q000295:instrumentation*; D013057:Spectrum Analysis / Q000295:instrumentation*
chemical_list: D006454:Hemoglobins