title: Distribution of resveratrol metabolites in liver, adipose tissue, and skeletal muscle in rats fed different doses of this polyphenol.
abstract: This study aimed to characterize resveratrol metabolite profiles in liver, skeletal muscle, and adipose tissue in rats treated for 6 weeks with 6, 30, or 60 mg of trans-resveratrol/kg body weight/d. Resveratrol metabolites were quantified by liquid chromatography-tandem mass spectrometry. The greatest number of metabolites was found in liver followed by adipose tissue. A great number of metabolites in muscle was below the limit of detection. The amounts of sulfate conjugates tended to increase when resveratrol dosage was enhanced, while the glucuronide ones increased only between 6 and 30 mg/kg/d. Microbiota metabolites were detected in higher amounts than resveratrol conjugates in liver, while the opposite occurred in adipose tissue and muscle. So, the largest amounts of resveratrol metabolites were found in liver, intermediate amounts in adipose tissue, and the lowest amounts in muscle. Sulfate conjugates, but not glucuronides, showed a dose-response pattern. Microbiota metabolites were predominant in liver.
authors.affiliation: Department of Nutrition and Food Science, XaRTA, INSA, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Andres-Lacueva
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Macarulla
authors.forename: Maria
authors.initials: M
authors.lastname: Rotches-Ribalta
authors.forename: María
authors.initials: M
authors.lastname: Boto-Ordóñez
authors.forename: Mireia
authors.initials: M
authors.lastname: Urpi-Sarda
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Rodríguez
authors.forename: María P
authors.initials: MP
authors.lastname: Portillo
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D000818:Animals; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D008297:Male; D054892:Metagenome; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D059808:Polyphenols / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*
chemical_list: D059808:Polyphenols; D013267:Stilbenes; D000077185:Resveratrol