title: [Effect of berberine on HL-60 cell proliferation, apoptosis and vascular endothelial growth factor receptor 2 expression].
abstract: This study was aimed to investigate the effect of berberine on the proliferation and apoptosis of HL-60 cells, and the expression of vascular endothelial growth factor receptor 2 (VEGFR2) in HL-60 cells. Berberine (6 - 96 µg/ml) was added to the HL-60 cell line culture medium, the CCK-8 method was used to reveal the inhibitory effect on cell proliferation, the flow cytometry was used to determine the apoptosis rate and cell cycle in HL-60 cells treated with berberine. The expression of VEGFR2 mRNA and protein were examined by RT-PCR and Western blot respectively. The results showed that the berberine inhibited the proliferation of HL-60 cells and induced their apoptosis in dose- and time-dependent manners. With the increased concentration of berberine, the percentage of HL-60 cells in G(1) phase of cycle increased significantly, and the percentage of HL-60 cells in S phase decreased significantly. The expression of mRNA and protein of VEGFR2 decreased with the increased concentration of berberine. It is concluded that the berberine can inhibit HL-60 cell proliferation and induce HL-60 cell apoptosis. The expression of mRNA and proteins of VEGFR2 decreased after treatment with berberine.
authors.affiliation: Department of Pediatrics, Affliated Hospital of Guangdong Medical College, Zhanjiang, China.
authors.forename: Shao-Zhu
authors.initials: SZ
authors.lastname: Lin
authors.forename: Ming-Zhen
authors.initials: MZ
authors.lastname: Chen
authors.forename: Xiu-Lan
authors.initials: XL
authors.lastname: Huang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001599:Berberine / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D005434:Flow Cytometry; D015973:Gene Expression Regulation, Leukemic; D018922:HL-60 Cells; D006801:Humans; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism*
chemical_list: D001599:Berberine; D040301:Vascular Endothelial Growth Factor Receptor-2