title: Calbindin controls release probability in ventral tegmental area dopamine neurons.
abstract: Relatively little is known about the molecular control of midbrain dopamine release. Using high-fidelity imaging of pHluorin-tagged vesicular monoamine transporter 2 in dopamine neurons, we found that exocytosis was more loosely coupled to calcium entry than in fast synapses. In ventral tegmental area neurons, this allows exocytosis to be efficiently controlled by a native fast calcium buffer, calbindin-D28k, maintaining a lower vesicular release probability compared with substantia nigra neurons.
authors.affiliation: Department of Biochemistry, Weill Cornell Medical College, New York, NY, USA. taryan@med.cornell.edu
authors.forename: Ping-Yue
authors.initials: PY
authors.lastname: Pan
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Ryan
mesh_terms: D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D004298:Dopamine / Q000378:metabolism*; D059290:Dopaminergic Neurons / Q000378:metabolism*; D005089:Exocytosis; D007150:Immunohistochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D014162:Transfection; D017557:Ventral Tegmental Area / Q000378:metabolism*
chemical_list: C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D004298:Dopamine