title: Sustainable release of vancomycin, gentamicin and lidocaine from novel electrospun sandwich-structured PLGA/collagen nanofibrous membranes.
abstract: This study investigated the in vitro release of vancomycin, gentamicin, and lidocaine from novel electrospun sandwich-structured polylactide-polyglycolide (PLGA)/collagen nanofibrous membranes. For the electrospinning of biodegradable membranes, PLGA/collagen and PLGA/vancomycin/gentamicin/lidocaine were separately dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP). They were then electrospun into sandwich structured membranes, with PLGA/collagen for the surface layers and PLGA/drugs for the core layer. After electrospinning, an elution method and HPLC assay were employed to characterize the in vitro release rates of the pharmaceutics over a 30-day period. The experiment showed that biodegradable nanofibrous membranes released high concentrations of vancomycin and gentamicin (well above the minimum inhibition concentration) for 4 and 3 weeks, respectively, and lidocaine for 2 weeks. A bacterial inhibition test was carried out to determine the relative activity of the released antibiotics. The bioactivity of vancomycin and gentamicin ranged from 30% to 100% and 37% to 100%, respectively. In addition, results indicated that the nanofibrous membranes were functionally active in responses in human fibroblasts. By adopting the electrospinning technique, we will be able to manufacture biodegradable biomimetic nanofibrous extracellular membranes for long-term drug delivery of various pharmaceuticals.
authors.affiliation: Department of Mechanical Engineering, Chang Gung University, Tao-Yuan,Taiwan.
authors.forename: Dave W
authors.initials: DW
authors.lastname: Chen
authors.forename: Yung-Heng
authors.initials: YH
authors.lastname: Hsu
authors.forename: Jun-Yi
authors.initials: JY
authors.lastname: Liao
authors.forename: Shih-Jung
authors.initials: SJ
authors.lastname: Liu
authors.forename: Jan-Kan
authors.initials: JK
authors.lastname: Chen
authors.forename: Steve Wen-Neng
authors.initials: SW
authors.lastname: Ueng
mesh_terms: D000779:Anesthetics, Local / Q000008:administration & dosage / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000737:chemistry* / Q000633:toxicity; D003692:Delayed-Action Preparations; D052978:Disk Diffusion Antimicrobial Tests; D004337:Drug Carriers; D004339:Drug Compounding; D004355:Drug Stability; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005347:Fibroblasts / Q000187:drug effects; D005839:Gentamicins / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D007223:Infant; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry* / Q000633:toxicity; D008012:Lidocaine / Q000008:administration & dosage / Q000737:chemistry*; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests; D057139:Nanofibers; D036103:Nanotechnology; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000633:toxicity; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D020005:Propanols / Q000737:chemistry; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013678:Technology, Pharmaceutical / Q000379:methods; D014640:Vancomycin / Q000008:administration & dosage / Q000737:chemistry*; D006845:Hydrocarbons, Fluorinated
chemical_list: D000779:Anesthetics, Local; D000900:Anti-Bacterial Agents; D003094:Collagen; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005839:Gentamicins; D019344:Lactic Acid; D008012:Lidocaine; D008567:Membranes, Artificial; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D020005:Propanols; D012997:Solvents; D014640:Vancomycin; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated