title: Aspergillus niger DLFCC-90 rhamnoside hydrolase, a new type of flavonoid glycoside hydrolase.
abstract: A novel rutin-α-L-rhamnosidase hydrolyzing α-L-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the α-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13.
authors.affiliation: College of Biotechnology, Dalian Polytechnic University, China.
authors.forename: Tingqiang
authors.initials: T
authors.lastname: Liu
authors.forename: Hongshan
authors.initials: H
authors.lastname: Yu
authors.forename: Chunzhi
authors.initials: C
authors.lastname: Zhang
authors.forename: Mingchun
authors.initials: M
authors.lastname: Lu
authors.forename: Yongzhe
authors.initials: Y
authors.lastname: Piao
authors.forename: Masashi
authors.initials: M
authors.lastname: Ohba
authors.forename: Minqian
authors.initials: M
authors.lastname: Tang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Yuan
authors.forename: Shenghua
authors.initials: S
authors.lastname: Wei
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
authors.forename: Anzhou
authors.initials: A
authors.lastname: Ma
authors.forename: Xue
authors.initials: X
authors.lastname: Feng
authors.forename: Siqing
authors.initials: S
authors.lastname: Qin
authors.forename: Chisato
authors.initials: C
authors.lastname: Mukai
authors.forename: Akira
authors.initials: A
authors.lastname: Tsuji
authors.forename: Fengxie
authors.initials: F
authors.lastname: Jin
mesh_terms: D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D044950:Flavanones / Q000378:metabolism*; D015870:Gene Expression; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D006569:Hesperidin / Q000378:metabolism*; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics; D012431:Rutin / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004271:DNA, Fungal; D044950:Flavanones; D012431:Rutin; D006569:Hesperidin; D006026:Glycoside Hydrolases; C005274:naringin