title: [Component I from Agkistrodon acutus venom induces apoptosis of K562/A02 cells by promoting caspase 3 expression].
abstract: To investigate the effects of component I from Agkistrodon acutus venom (AAVC-I) on the biological features of chronic myeloid leukemia cells, K562/A02 leukemia cells were cultured in the presence of AAVC-I (6.25 - 100 µg/ml) and the proliferation status was assayed by CCK-8 method. Morphological changes were observed by inversed microscope after Giemsa and Hochest 33258 staining, and cell apoptosis was detected by flow cytometry. Caspase 3 activity was tested by using Chromogenic Activity Assay Kit. The results showed that AAVC-I inhibited the growth of K562/A02 cells in time- and concentration-dependant manners, and the IC(50) at 48 h was 30.988 µg/ml. Giemsa and Hochest 33258 staining showed the typical apoptotic features in K562/A02 cells after induction with AAVC-I for 48 h. Flow cytometric analysis revealed that the percentage of the apoptotic cells reached from 0.88 up to 53.66 as the treated concentration was elevated from 0 to 50 µg/ml. Compared with the control group, the expression of caspase 3 in the tested group was enhanced in a dose-dependent manner (P < 0.05). It is concluded that AAVC-I can effectively inhibit the growth and promote apoptosis of K562/A02 cells. Elevated expression of caspase-3 may be attributed to the apoptosis of K562/A02 cells.
authors.affiliation: Department of Pathophysioloy, Wuhu Medical College, Wuhu, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhou
authors.forename: Gen-Bao
authors.initials: GB
authors.lastname: Zhang
authors.forename: Ting
authors.initials: T
authors.lastname: Duan
authors.forename: Jue
authors.initials: J
authors.lastname: Zhou
authors.forename: Juan
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D003435:Crotalid Venoms / Q000494:pharmacology*; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology
chemical_list: C031442:Agkistrodon venoms; D003435:Crotalid Venoms; C505407:CASP3 protein, human; D053148:Caspase 3