title: Cell surface glycoprotein profiling of cancer cells based on bioorthogonal chemistry.
abstract: Bioorthogonal chemistry refers to chemical reactions that can occur within a living system without altering native biochemical processes. Applications of this concept extend to studies on a group of biomolecules that includes glycans, proteins, and lipids. In this study, a strategy for isolating cell surface glycoproteins and based on bioorthogonal chemistry was employed to identify new cancer-related glycoproteins. A novel alkyne reagent containing one disulfide bond was synthesized for the enrichment of glycoproteins metabolized with peracetylated N-azidoacetylmannosamine, which was applied on three different cancer cell lines, and all isolated proteins were analyzed by high-performance liquid chromatography-tandem mass spectrometry. The strategy of purifying cell surface glycoproteins introduced in this article was shown to be reliable, and a total of 56 cell surface glycoproteins were identified. Neuronal cell adhesion molecule was found uniquely expressed in A549 lung adenocarcinoma, and its expression in non-small-cell lung carcinomas was detected by immunohistochemistry. Furthermore, a significant increase of neuronal cell adhesion molecule expression was identified in non-small-cell lung adenocarcinoma compared with adjacent noncancerous tissues, and could be a novel potential target and marker in cancer treatment and detection.
authors.affiliation: College of Pharmacy and College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Peng-Wei
authors.initials: PW
authors.lastname: Pan
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Hou
authors.forename: Ze
authors.initials: Z
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Bai
authors.forename: Mei-rong
authors.initials: MR
authors.lastname: Cao
authors.forename: Ting
authors.initials: T
authors.lastname: Sun
authors.forename: Gang
authors.initials: G
authors.lastname: Bai
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D008562:Membrane Glycoproteins