title: STIM1 regulates calcium signaling in taste bud cells and preference for fat in             mice.
abstract: Understanding the mechanisms underlying oro-gustatory detection of dietary fat is critical for the prevention and treatment of obesity. The lipid-binding glycoprotein CD36, which is expressed by circumvallate papillae (CVP) of the mouse tongue, has been implicated in oro-gustatory perception of dietary lipids. Here, we demonstrate that stromal interaction molecule 1 (STIM1), a sensor of Ca(2+) depletion in the endoplasmic reticulum, mediates fatty acid-induced Ca(2+) signaling in the mouse tongue and fat preference. We showed that linoleic acid (LA) induced the production of arachidonic acid (AA) and lysophosphatidylcholine (Lyso-PC) by activating multiple phospholipase A2 isoforms via CD36. This activation triggered Ca(2+) influx in CD36-positive taste bud cells (TBCs) purified from mouse CVP. LA also induced the production of Ca(2+) influx factor (CIF). STIM1 was found to regulate LA-induced CIF production and the opening of multiple store-operated Ca(2+) (SOC) channels. Furthermore, CD36-positive TBCs from Stim1-/- mice failed to release serotonin, and Stim1-/- mice lost the spontaneous preference for fat that was observed in wild-type animals. Our results suggest that fatty acid-induced Ca(2+) signaling, regulated by STIM1 via CD36, might be implicated in oro-gustatory perception of dietary lipids and the spontaneous preference for fat.
authors.affiliation: Unité Propre de L’Enseignement Supérieure Lipides and Signalisation Cellulaire Equipe d’Accueil 4183, Faculté des sciences de la vie, terre et environnement, Dijon, France.
authors.forename: Gado
authors.initials: G
authors.lastname: Dramane
authors.forename: Souleymane
authors.initials: S
authors.lastname: Abdoul-Azize
authors.forename: Aziz
authors.initials: A
authors.lastname: Hichami
authors.forename: Timo
authors.initials: T
authors.lastname: Vögtle
authors.forename: Simon
authors.initials: S
authors.lastname: Akpona
authors.forename: Christophe
authors.initials: C
authors.lastname: Chouabe
authors.forename: Hassimi
authors.initials: H
authors.lastname: Sadou
authors.forename: Bernhard
authors.initials: B
authors.lastname: Nieswandt
authors.forename: Philippe
authors.initials: P
authors.lastname: Besnard
authors.forename: Naim Akhtar
authors.initials: NA
authors.lastname: Khan
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000235:genetics / Q000378:metabolism; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology*; D004041:Dietary Fats / Q000494:pharmacology*; D005518:Food Preferences / Q000502:physiology*; D019787:Linoleic Acid / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D012701:Serotonin / Q000235:genetics / Q000378:metabolism; D000071737:Stromal Interaction Molecule 1; D013650:Taste Buds / Q000378:metabolism*; D055697:Taste Perception / Q000502:physiology*
chemical_list: D018955:CD36 Antigens; D015220:Calcium Channels; D004041:Dietary Fats; D008562:Membrane Glycoproteins; C100757:Stim1 protein, mouse; D000071737:Stromal Interaction Molecule 1; D016718:Arachidonic Acid; D012701:Serotonin; D019787:Linoleic Acid