title: A highly sensitive electrochemical biosensor based on zinc oxide nanotetrapods for L-lactic acid detection.
abstract: An amperometric biosensor based on zinc oxide (ZnO) nanotetrapods was designed to detect L-lactic acid. The lactate oxidase was immobilized on the surface of ZnO nanotetrapods by electrostatic adsorption. Unlike traditional detectors, the special four-leg individual ZnO nanostructure, as an adsorption layer, provides multiterminal charge transfer channels. Furthermore, a large amount of ZnO tetrapods are randomly stacked to form a three-dimensional network naturally that facilitates the exchange of electrons and ions in the phosphate buffer solution. Utilizing amperometric response measurements, the prepared ZnO nanotetrapod L-lactic acid biosensor displayed a detection limit of 1.2 μM, a low apparent Michaelis-Menten constant of 0.58 mM, a high sensitivity of 28.0 μA cm(-2) mM(-1) and a good linear relationship in the range of 3.6 μM-0.6 mM for the L-lactic acid detection. This study shows that the biosensor based on ZnO tetrapod nanostructures is highly sensitive and able to respond rapidly in detecting lactic acid.
authors.affiliation: State Key Laboratory for Advanced Metals and Materials, School of Materials Science & Engineering, University of Science & Technology Beijing, Beijing 100083, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lei
authors.forename: Ning
authors.initials: N
authors.lastname: Luo
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Yan
authors.forename: Yanguang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Gong
authors.initials: G
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D019344:Lactic Acid / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D005465:Fluorocarbon Polymers; D019344:Lactic Acid; C040402:perfluorosulfonic acid; D006046:Gold; D015034:Zinc Oxide