title: Little evidence of systemic and adipose tissue inflammation in overweight individuals(†).
abstract: BACKGROUND
  The effect of weight loss by diet alone or diet in conjunction with exercise on low-grade inflammation in non-obese (overweight) individuals is not known.
  
  
  OBJECTIVE
  Test the hypothesis that 24 weeks of moderate calorie restriction (CR; 25%) by diet only or with aerobic exercise would reduce markers of systemic inflammation and attenuate inflammation gene expression in subcutaneous adipose tissue.
  
  
  METHODS
  Randomized controlled trial.
  
  
  METHODS
  Institutional Research Center.
  
  
  METHODS
  Thirty-five overweight (body mass index: 27.8 ± 0.7 kg/m(2)) but otherwise healthy participants (16M/19F) completed the study.
  
  
  METHODS
  PARTICIPANTS were randomized to either CR (25% reduction in energy intake, n = 12), caloric restriction + exercise (CR + EX: 12.5% reduction in energy intake + 12.5% increase in exercise energy expenditure, n = 12), or control (healthy weight-maintenance diet, n = 11) for 6 months.
  
  
  METHODS
  Fasting serum markers of inflammation [leptin, highly sensitive C-reactive protein (hsCRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), adiponectin] and inflammation-related genes [CD68, IL-6, TNF-α, macrophage migration inhibitory factor (MIF), monocyte chemoattractant protein-1 (MCP-1), adiponectin, plasminogen activator inhibitor-1 (PAI-1)] in subcutaneous adipose tissue.
  
  
  RESULTS
  CR and CR + EX lost similar amounts of body weight (-10 ± 1%), fat mass (-24 ± 3%), visceral fat (-27 ± 3%), and had increased insulin sensitivity (CR: 40 ± 20%, CR + EX: 66 ± 22%). Leptin was significantly decreased from baseline (p < 0.001) in both groups however TNF-α and IL-6 were not changed. hsCRP was decreased in CR + EX. There was no change in the expression of genes involved in macrophage infiltration (CD68, MIF MCP-1, PAI-1) or inflammation (IL-6, TNF-α, adiponectin) in either CR or CR + EX.
  
  
  CONCLUSIONS
  A 10% weight loss with a 25% CR diet alone or with exercise did not impact markers of systemic inflammation or the expression of inflammation-related adipose genes in overweight individuals.
authors.affiliation: Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
authors.forename: Charmaine S
authors.initials: CS
authors.lastname: Tam
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Covington
authors.forename: Eric
authors.initials: E
authors.lastname: Ravussin
authors.forename: Leanne M
authors.initials: LM
authors.lastname: Redman