title: An optimized two-finger archive for ZFN-mediated gene targeting.
abstract: The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.
authors.affiliation: Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, USA.
authors.forename: Ankit
authors.initials: A
authors.lastname: Gupta
authors.forename: Ryan G
authors.initials: RG
authors.lastname: Christensen
authors.forename: Amy L
authors.initials: AL
authors.lastname: Rayla
authors.forename: Abirami
authors.initials: A
authors.lastname: Lakshmanan
authors.forename: Gary D
authors.initials: GD
authors.lastname: Stormo
authors.forename: Scot A
authors.initials: SA
authors.lastname: Wolfe
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D053903:DNA Breaks, Double-Stranded; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D015027:Zebrafish; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D004706:Endodeoxyribonucleases