title: Measuring the kinetics of amyloid fibril elongation using quartz crystal microbalances.
abstract: Kinetic measurements of amyloid growth provide insight into the free energy landscape of this supramolecular process and are crucial in the search for potent inhibitors of the main disorders with which it is associated, including Alzheimer's and Parkinson's diseases and Type II diabetes. In recent years, a new class of surface-bound biosensor assays, e.g., those based on surface plasmon resonance (SPR) and the quartz crystal microbalance (QCM) have been established as extremely valuable tools for kinetic measurements of amyloid formation. Here we describe detailed protocols of how QCM techniques can be used to monitor the elongation of amyloid fibrils in real time and to study the influence of external factors on the kinetics of amyloid growth with unprecedented accuracy.
authors.affiliation: Nanoscience Centre, University of Cambridge, Cambridge, UK. ab761@cam.ac.uk
authors.forename: Alexander K
authors.initials: AK
authors.lastname: Buell
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Dobson
authors.forename: Mark E
authors.initials: ME
authors.lastname: Welland
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D016229:Amyloid beta-Peptides / Q000737:chemistry; D000818:Animals; D002417:Cattle; D003538:Cystamine / Q000737:chemistry; D004789:Enzyme Activation; D006046:Gold / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000737:chemistry; D007700:Kinetics; D010446:Peptide Fragments / Q000737:chemistry; D019869:Phosphatidylinositol 3-Kinases / Q000737:chemistry / Q000378:metabolism; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D058525:Quartz Crystal Microbalance Techniques / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties; D051844:alpha-Synuclein / Q000737:chemistry; D018909:src Homology Domains
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D007328:Insulin; D010446:Peptide Fragments; D051844:alpha-Synuclein; C075222:amyloid beta-protein (1-42); D006046:Gold; D019869:Phosphatidylinositol 3-Kinases; D003538:Cystamine