title: New records of Anopheles homunculus in central and Serra do Mar biodiversity corridors of the Atlantic Forest, Brazil.
abstract: Two new records of Anopheles homunculus in the eastern part of the Atlantic Forest are reported. This species was found for the first time in Barra do Ouro district, Maquiné municipality, Rio Grande do Sul state, located in the southern limit of the Atlantic Forest. The 2nd new record was in the Serra Bonita Reserve, Camacan municipality, southeast Bahia state. These records extend the geographical distribution of An. homunculus, suggesting that the species may be widely distributed in coastal areas of the Atlantic Forest. It is hypothesized that the disjunct distribution of the species may be caused by inadequate sampling, and also difficulties in species identification based only on female external characteristics. Species identification was based on morphological characters of the male, larva, and pupa, and corroborated by DNA sequence analyses, employing data from both 2nd internal transcribed spacer of nuclear ribosomal DNA and of mitochondrial cytochrome c oxidase subunit I.
authors.affiliation: Divisão de Vigilância Ambiental em Saúde, Centro Estadual de Vigilância em Saúde, Secretaria da Saúde do Estado do Rio Grande do Sul, 90650-090 Porto Alegre, RS, Brazil.
authors.forename: Jader C
authors.initials: JC
authors.lastname: da Cardoso
authors.forename: Eduardo S
authors.initials: ES
authors.lastname: Bergo
authors.forename: Tatiane M P
authors.initials: TM
authors.lastname: Oliveira
authors.forename: Denise C
authors.initials: DC
authors.lastname: Sant'ana
authors.forename: Maysa T
authors.initials: MT
authors.lastname: Motoki
authors.forename: Maria Anice M
authors.initials: MA
authors.lastname: Sallum
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics; D044822:Biodiversity; D001938:Brazil; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D050259:Genes, Mitochondrial; D008297:Male
chemical_list: D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV