title: Use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro.
abstract: Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potential pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and Madin-Darby canine kidney cells were detected by confocal microscopy and flow cytometry.
authors.affiliation: Erasmus Medical Centre, Dr. Molewaterplein 50, 3015GE Rotterdam, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Bodewes
authors.affiliation: Erasmus Medical Centre, Dr. Molewaterplein 50, 3015GE Rotterdam, The Netherlands.
authors.forename: N J
authors.initials: NJ
authors.lastname: Nieuwkoop
authors.affiliation: Erasmus Medical Centre, Dr. Molewaterplein 50, 3015GE Rotterdam, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Verburgh
authors.affiliation: Erasmus Medical Centre, Dr. Molewaterplein 50, 3015GE Rotterdam, The Netherlands.
authors.forename: R A M
authors.initials: RAM
authors.lastname: Fouchier
authors.affiliation: ViroClinics Biosciences, Dr. Molewaterplein 50, 3015GE Rotterdam, The Netherlands.|Erasmus Medical Centre, Dr. Molewaterplein 50, 3015GE Rotterdam, The Netherlands.
authors.forename: A D M E
authors.initials: ADME
authors.lastname: Osterhaus
authors.affiliation: ViroClinics Biosciences, Dr. Molewaterplein 50, 3015GE Rotterdam, The Netherlands.|Erasmus Medical Centre, Dr. Molewaterplein 50, 3015GE Rotterdam, The Netherlands.
authors.forename: G F
authors.initials: GF
authors.lastname: Rimmelzwaan
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D004285:Dogs; D005434:Flow Cytometry; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D005814:Genes, Viral; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000378:metabolism; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D018613:Microscopy, Confocal; D016865:Reassortant Viruses / Q000235:genetics / Q000378:metabolism; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein; C485184:enhanced green fluorescent protein