title: One-step labeling of degenerative neurons in unfixed brain tissue samples using Fluoro-Jade C.
abstract: Neurodegeneration is the underlying cause of a vast majority of neurological disorders and often a result of brain trauma, stroke, or neurotoxic insult. Here we describe a simple method for labeling degenerating neurons in unfixed brain tissue samples. This method could provide a new avenue for identifying and harvesting degenerative neurons from unfixed brain tissues for subsequent molecular analyses.
authors.affiliation: Division of Neurotoxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, United States. qiang.gu@fda.hhs.gov
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gu
authors.forename: Larry C
authors.initials: LC
authors.lastname: Schmued
authors.forename: Sumit
authors.initials: S
authors.lastname: Sarkar
authors.forename: Merle G
authors.initials: MG
authors.lastname: Paule
authors.forename: Bryan
authors.initials: B
authors.lastname: Raymick
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D019636:Neurodegenerative Diseases / Q000473:pathology*; D009930:Organic Chemicals; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C435731:fluoro jade