title: The protective role of vitamin E on gill and liver tissue histopathology and micronucleus frequencies in peripheral erythrocytes of Oreochromis niloticus exposed to deltamethrin.
abstract: Deltamethrin, is a commonly used pyrethroid pesticide. Vitamin E is a antioxidant that plays an important role in protecting cells against toxicity by inactivating free radicals generated following pesticides exposure. Therefore, it was evaluated whether deltamethrin induced histopathological changes and nuclear abnormalities using micronucleus test in Oreochromis niloticus, and the possible protective effect of vitamin E against deltamethrin inducing adverse effects in O. niloticus were investigated. Fish was fed with no pesticide+control diet, no pesticide+vitamin E-supplemented diet, 1.45μg/l deltamethrin+control diet, 1.45μg/l deltamethrin+vitamin E-supplemented diet for 30 days. Pesticide and diet quality made an impact on histopathological lesions. In treatments of deltamethrin, group fed with control diet showed much greater damage in comparison with group fed with vitamin E supplemented diet. Vitamin E decreased some histopathological changes induced by deltamethrin, but did not confer complete protection. Deltamethrin treatment has been shown to results in a significant increase in the frequency of micronucleus. However, coadministration of deltamethrin and vitamin E showed decrease in the frequency of micronucleus as compared to deltamethrin treated fish. Our results indicate that, the MN assay and histopathology can be used as bioassays for monitoring pollution in aquatic medium. On the other hand, it was observed that vitamin E decreased the genotoxicity and histopathological changes induced by deltamethrin.
authors.affiliation: Dicle University, Institute of Natural and Applied Sciences, Department of Biology, TR-21280 Diyarbakir, Turkey.
authors.forename: Yeter
authors.initials: Y
authors.lastname: Kan
authors.affiliation: Dicle University, Faculty of Science, Department of Biology, Section of Hydrobiology, TR-21280 Diyarbakir, Turkey. Electronic address: ecengiz@dicle.edu.tr.
authors.forename: Elif Ipek
authors.initials: EI
authors.lastname: Cengiz
authors.affiliation: Dicle University, Institute of Natural and Applied Sciences, TR 21280 Diyarbakir, Turkey.
authors.forename: Pelin
authors.initials: P
authors.lastname: Ugurlu
authors.affiliation: Cukurova University, Department of Aquaculture, Faculty of Fisheries, TR-01330 Adana, Turkey.
authors.forename: Mahmut
authors.initials: M
authors.lastname: Yanar
mesh_terms: D000818:Animals; D023681:Cichlids; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D005880:Gills / Q000187:drug effects / Q000473:pathology; D007306:Insecticides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D015162:Micronucleus Tests; D009570:Nitriles / Q000633:toxicity*; D020011:Protective Agents / Q000494:pharmacology*; D011722:Pyrethrins / Q000633:toxicity*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D007306:Insecticides; D009570:Nitriles; D020011:Protective Agents; D011722:Pyrethrins; D014810:Vitamin E; C017180:decamethrin