title: A new insect cell line from pupal ovary of Spodoptera exigua established by stimulation with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
abstract: A continuous cell line derived from the pupal ovary of Spodoptera exigua was established by treating primary cells with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Three days after treating cells with 3.0 μg/ml of MNNG, the cells formed a monolayer and were initially subcultured 60 d after the MNNG was removed, followed by subculturing for 30 passages. The established cell line, designated IOZCAS-Spex 12, consisted of a mixture of three types of cells, including spherical, spindle-shaped, and oval cells. The population doubling time of the cell line during its logarithmic growth phase was found to be 71 h. DNA amplification fingerprinting polymerase chain reaction analysis confirmed that the new cell line originated from S. exigua. Susceptibility of IOZCAS-Spex 12 cells to infection by certain nucleopolyhedroviruses was investigated. The results showed that the cell line was highly susceptible to infection by S. exigua nucleopolyhedrovirus and Autographa californica multiple nucleopolyhedrovirus, slightly susceptible to infection by Spodoptera litura nucleopolyhedrovirus, and not susceptible to infection by Helicoverpa armigera nucleopolyhedroviruses or Hyphantria cunea nucleopolyhedroviruses. The results of this study suggest that MNNG treatment may overcome existing limitations to obtaining continually proliferating cells and may open up the possibilities for immortalizing isolated insect cells.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. lix@ioz.ac.cn
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.forename: Qilian
authors.initials: Q
authors.lastname: Qin
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Jihong
authors.initials: J
authors.lastname: Zhang
authors.forename: Hongtuo
authors.initials: H
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Miao
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line / Q000166:cytology / Q000187:drug effects / Q000821:virology; D049109:Cell Proliferation; D005260:Female; D007304:Insect Viruses / Q000254:growth & development; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D010053:Ovary / Q000166:cytology; D018411:Spodoptera / Q000166:cytology* / Q000821:virology
chemical_list: D008769:Methylnitronitrosoguanidine