title: Oviposition and flight orientation response of Aedes aegypti to certain aromatic aryl hydrazono esters.
abstract: Aedes aegypti is a day-biting, highly anthropophilic mosquito and a potential vector of dengue and chikungunya in India. A. aegypti is a container breeder, generally oviposit in the stored and fresh water bodies, and discarded containers near residential areas that provide suitable habitats for oviposition by gravid females. The diurnal activity and endophilic nature of these mosquitoes have increased the frequency of contact with human being. Assured blood meal from human host in an infested area leads to increased disease occurrence. Gravid mosquitoes can potentially be lured to attractant-treated traps and could subsequently be killed with insecticides or growth regulators. In this direction, oviposition by A. aegypti females to aryl hydrazono esters (AHE)-treated bowls at 10 ppm concentration was tested in dual choice experiment, and their orientation response to these ester compounds was studied in Y-tube olfactometer. Among the esters tested, AHE-2, AHE-11 and AHE-12 elicited increased egg deposition with oviposition activity indices (OAI) of +0.39, +0.24 and +0.48, respectively, compared to control; in contrast, AHE-8, AHE-9 and AHE-10 showed negative oviposition response with OAI of -0.46, -0.35 and -0.29, respectively, at 10 mg/L. In the Y-tube olfactometer bioassay, AHE-2 attracted 60 % females compared to control, while to the odour of AHE-11 and AHE-12, about 70 % of the females were trapped in treated chambers. In contrast, only 27-30 % of gravid females entered the chamber releasing AHE-8, AHE-9 and AHE-10 odour plumes, while 70 % entered control chamber, evincing a possible non-preference of treatment odours as well as interference with olfactory receptors. These compounds have the potential for application as oviposition stimulants or deterrents for surveillance and control of mosquito population using ovitraps.
authors.affiliation: Defence Research & Development Establishment, Jhansi Road, Gwalior, 474 002, Madhya Pradesh, India.
authors.forename: Lopamudra
authors.initials: L
authors.lastname: Guha
authors.forename: T
authors.initials: T
authors.lastname: Seenivasagan
authors.forename: Prabal
authors.initials: P
authors.lastname: Bandyopadhyay
authors.forename: S Thanvir
authors.initials: ST
authors.lastname: Iqbal
authors.forename: Manisha
authors.initials: M
authors.lastname: Sathe
authors.forename: Pratibha
authors.initials: P
authors.lastname: Sharma
authors.forename: B D
authors.initials: BD
authors.lastname: Parashar
authors.forename: M P
authors.initials: MP
authors.lastname: Kaushik
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D005260:Female; D005426:Flight, Animal / Q000187:drug effects*; D006841:Hydrocarbons, Aromatic / Q000138:chemical synthesis* / Q000494:pharmacology*; D007306:Insecticides / Q000134:cerebrospinal fluid* / Q000494:pharmacology*; D015394:Molecular Structure; D010058:Oviposition / Q000187:drug effects*
chemical_list: D006841:Hydrocarbons, Aromatic; D007306:Insecticides