title: The effects of Ce on the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells in vitro.
abstract: The effects of Ce on the proliferation, osteogenic differentiation and mineralization function of a murine preosteoblast cell line MC3T3-E1 in vitro were investigated at cell and molecular levels. The results showed that Ce promoted the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells at concentrations of 0.0001, 0.001, 0.01, 0.1 and 1 μM, but turned to inhibit the proliferation, osteogenic differentiation and mineralization function at concentrations of 10, 100 and 1000 μM. Ce displayed the up-regulation of Runx2, BMP2, ALP, BSP, Col I and OCN genes at concentrations of 0.0001 and 0.1 μM; these genes were down-regulated in the MC3T3-E1 cells treated with 1000 μM Ce. The expression of BMP2, Runx2 and OCN proteins was promoted by Ce at concentrations of 0.0001 and 0.1 μM, but these proteins were down-regulated after 1000 μM Ce treatment. The results suggest that Ce likely up-regulates or down-regulates the expression of Runx2, which subsequently up- or down-regulates OB marker genes Col I and BMP2 at early stages and ALP and OCN at later stages of differentiation, thus causing to promote or inhibit the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells.
authors.affiliation: College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, People's Republic of China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
authors.forename: Jinchao
authors.initials: J
authors.lastname: Zhang
authors.forename: Yaping
authors.initials: Y
authors.lastname: Li
authors.forename: Shuxiang
authors.initials: S
authors.lastname: Wang
authors.forename: MengSu
authors.initials: M
authors.lastname: Yang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002563:Cerium / Q000494:pharmacology*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; C493716:Runx2 protein, mouse; D002563:Cerium