title: Degradation of 17α-methyltestosterone by Rhodococcus sp. and Nocardioides sp. isolated from a masculinizing pond of Nile tilapia fry.
abstract: 17α-Methyltestosterone (MT), a synthetic anabolic androgenic steroid, is widely used in aquafarming for the production of an all male fish population such as Nile tilapia. This study isolated, identified and characterized MT-degrading bacteria in the sediment and water from a masculinizing pond of Nile tilapia fry. Based on the phylogeny, physiological properties and cell morphology, the three isolated MT-degrading bacteria were related closely to Rhodococcus equi, Nocardioides aromaticivorans, and Nocardioides nitrophenolicus. Growth of the three isolated strains was found to be inhibited for MT concentrations in the range of 1.0-10mg/L. The inhibition of cell growth was found to be modeled using the Haldane's substrate inhibition model. The kinetic constants ranged from 0.13 to 0.19h(-1) for μ(max), 0.7-24.8mg/L for K(s) and 19.6-76.2mg/L for K(i). Androgenic activity using β-galactosidase assay showed that all strains degraded MT to the products with no androgenic potency.
authors.affiliation: International Postgraduate Programs in Environmental Management, Graduate School, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Supreeda
authors.initials: S
authors.lastname: Homklin
authors.forename: Say Kee
authors.initials: SK
authors.lastname: Ong
authors.forename: Tawan
authors.initials: T
authors.lastname: Limpiyakorn
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D008777:Methyltestosterone / Q000378:metabolism*; D009615:Nocardia / Q000378:metabolism*; D012240:Rhodococcus / Q000378:metabolism*; D017210:Tilapia / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D008777:Methyltestosterone