title: Comparison and Optimization of Different Methods for the In Vitro Production of Plasmodium falciparum Gametocytes.
abstract: The generation of sexually committed parasites (gametocytogenesis) is poorly understood in malaria. If the mechanisms regulating this process were elucidated, new opportunities for blocking malaria transmission could be revealed. Here we compare several methods described previously for the in vitro production of Plasmodium falciparum gametocytes. Our approach relies on the combination of several factors that we demonstrated as impacting on or being critical to gametocytogenesis. An improved method has been developed for the in vitro production of P. falciparum gametocytes as the first step toward obtaining adequate numbers of pure gametocytes for in vitro studies, such as, for example, the identification of transmission blocking drugs.
authors.affiliation: Malaria Discovery Performance Unit, GlaxoSmithKline R&D, Tres Cantos Medicine Development Campus, C/Severo Ochoa No. 2, Tres Cantos, 28760 Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Roncalés
authors.forename: Jaume
authors.initials: J
authors.lastname: Vidal-Mas
authors.forename: Didier
authors.initials: D
authors.lastname: Leroy
authors.forename: Esperanza
authors.initials: E
authors.lastname: Herreros