title: B16-F10 melanoma cells contribute to the new formation of blood vessels in the chick embryo chorioallantoic membrane through vasculogenic mimicry.
abstract: Grafting of mammalian cells and tissues to the chick embryo chorioallantoic membrane (CAM) is a well-established experimental system to evaluate many different parameters of tumor growth, and B16-F10 murine melanoma cell line has been successfully used to study metastatic process in the CAM assay. The aim of this study was to demonstrate the capability of B16-F10 melanoma cells to contribute to the new formation of host blood vessels through a vasculogenic mimicry mode. Results have shown that B16-F10 melanoma cells are able to form in 4 days macroscopic tumor masses and induce a strong angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2. Moreover, tumor cells are able to cross the chorionic epithelium, and to move beneath in the mesenchyme to form tumor masses immunoreactive to specific antibodies anti-S100 and anti-MART-1/Melan-A. Finally, we have shown that CAMs new-formed blood vessels are lined by both pigmented melanoma cells and cells immunoreactive to MART-1/Melan-A and PAS, suggesting the occurrence of a vasculogenic mimicry process.
authors.affiliation: Section of Human Anatomy and Histology, Department of Basic Medical Sciences, University of Bari Medical School, Piazza G. Cesare, 11, Policlinico, 70124 Bari, Italy. ribatti@anatomia.uniba.it
authors.forename: Domenico
authors.initials: D
authors.lastname: Ribatti
authors.forename: Beatrice
authors.initials: B
authors.lastname: Nico
authors.forename: Anca Maria
authors.initials: AM
authors.lastname: Cimpean
authors.forename: Marius
authors.initials: M
authors.lastname: Raica
authors.forename: Enrico
authors.initials: E
authors.lastname: Crivellato
authors.forename: Simona
authors.initials: S
authors.lastname: Ruggieri
authors.forename: Angelo
authors.initials: A
authors.lastname: Vacca
mesh_terms: D000482:Allantois / Q000098:blood supply / Q000378:metabolism; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002465:Cell Movement; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D004727:Endothelium / Q000378:metabolism; D058965:MART-1 Antigen / Q000378:metabolism; D008546:Melanoma, Experimental; D008648:Mesoderm / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D009418:S100 Proteins / Q000378:metabolism; D013997:Time Factors
chemical_list: D014408:Biomarkers, Tumor; D058965:MART-1 Antigen; D009418:S100 Proteins