title: Normalizing the metabolic phenotype after myocardial infarction: impact of subchronic high fat feeding.
abstract: The normal heart relies primarily on the oxidation of fatty acids (FA) for ATP production, whereas during heart failure (HF) glucose utilization increases, implying pathological changes to cardiac energy metabolism. Despite the noted lipotoxic effects of elevating FA, our work has demonstrated a cardioprotective effect of a high fat diet (SAT) when fed after myocardial infarction (MI), as compared to normal chow (NC) fed cohorts. This data has suggested a mechanistic link to energy metabolism. The goal of this study was to determine the impact of SAT on the metabolic phenotype of the heart after MI. Male Wistar rats underwent coronary ligation surgery (MI) and were evaluated after 8 weeks of SAT. Induction of MI was verified by echocardiography. LV function assessed by in vivo hemodynamic measurements revealed improvements in the MI-SAT group as compared to MI-NC. Perfused working hearts revealed a decrease in cardiac work in MI-NC that was improved in MI-SAT. Glucose oxidation was increased and FA oxidation decreased in MI-NC compared to shams suggesting an alteration in the metabolic profile that was ameliorated by SAT. (31)P NMR analysis of Langendorff perfused hearts revealed no differences in PCr:ATP indicating no overt energy deficit in MI groups. Phospho-PDH and PDK(4) were increased in MI-SAT, consistent with a shift towards fatty acid oxidation (FAO). Overall, these results support the hypothesis that SAT post-infarction promotes a normal metabolic phenotype that may serve a cardioprotective role in the injured heart.
authors.affiliation: Dept of Physiology & Biophysics, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106-4970, USA.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Berthiaume
authors.forename: Martin E
authors.initials: ME
authors.lastname: Young
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Chen
authors.forename: Tracy A
authors.initials: TA
authors.lastname: McElfresh
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.forename: Margaret P
authors.initials: MP
authors.lastname: Chandler
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D004452:Echocardiography; D004734:Energy Metabolism; D006439:Hemodynamics; D008297:Male; D055442:Metabolome; D009203:Myocardial Infarction / Q000378:metabolism* / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism; D010641:Phenotype; D010725:Phosphocreatine / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D051381:Rats
chemical_list: D011768:Pyruvate Dehydrogenase Complex; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D011494:Protein Kinases; C403287:pyruvate dehydrogenase kinase 4