title: Genetic diversity and frequency of bovine viral diarrhea virus (BVDV) detected in cattle in Turkey.
abstract: The aim of this study was to investigate the frequency and diversity of bovine viral diarrhea viruses (BVDV) infecting cattle in Turkey. A total of 1124 bovine blood samples from 19 farms in 4 different Turkish regions were tested by antigen capture ELISA (ACE). BVDV antigen was found in 26 samples from 13 farms. Only 20 of the 26 initial test positive cattle were available for retesting. Of these, 6 of 20 tested positive for BVDV, by ACE and real-time RT-PCR, one month after initial testing. Phylogenetic analysis, based on comparison of the E2 or the 5'UTR coding regions, from 19 of the 26 initial positive samples, indicated that 17 belonged to the BVDV-1 genotype and 2 to the BVDV-2 genotype. Comparison of 5'UTR sequences segregated 8 BVDV-1 strains (strains 5, 6, 10, 11, 12, 13, 17, and 19) to the BVDV1f, 1 strain (strain 8) to the BVDV1i and 1 strain (strain 14) to the BVDV1d subgenotypes. One strain (strain 4) did not group with other subgenotypes but was closer to the BVDV1f. The remaining 6 BVDV-1 strains (strains 1, 2, 3, 7, 9, and 18) segregated to a novel subgenotype. The E2 sequence comparison results were similar, with the exception that strain 5 grouped with the novel subgenotype rather than BVDV1f subgenotype. It appears that among the diverse BVDV strains in circulation there may be a subgenotype that is unique to Turkey. This should be considered in the design of diagnostics and vaccines to be used in Turkey.
authors.affiliation: University of Istanbul, Veterinary Faculty, Department of Virology, Avcilar, Istanbul, Turkey. hyilmaz@istanbul.edu.tr
authors.forename: Huseyin
authors.initials: H
authors.lastname: Yilmaz
authors.forename: Eda
authors.initials: E
authors.lastname: Altan
authors.forename: Julia
authors.initials: J
authors.lastname: Ridpath
authors.forename: Nuri
authors.initials: N
authors.lastname: Turan
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D000956:Antigens, Viral / Q000097:blood; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology / Q000821:virology; D002417:Cattle / Q000821:virology*; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004797:Enzyme-Linked Immunosorbent Assay; D014644:Genetic Variation; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods; D010802:Phylogeny; D014421:Turkey / Q000453:epidemiology; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D000956:Antigens, Viral; D014759:Viral Envelope Proteins