title: A native chemical ligation approach for combinatorial assembly of affibody molecules.
abstract: Affinity molecules labeled with different reporter groups, such as fluorophores or radionuclides, are valuable research tools used in a variety of applications. One class of engineered affinity proteins is Affibody molecules, which are small (6.5 kDa) proteins that can be produced by solid phase peptide synthesis (SPPS), thereby allowing site-specific incorporation of reporter groups during synthesis. The Affibody molecules are triple-helix proteins composed of a variable part, which gives the protein its binding specificity, and a constant part, which is identical for all Affibody molecules. In the present study, native chemical ligation (NCL) has been applied for combinatorial assembly of Affibody molecules from peptide fragments produced by Fmoc SPPS. The concept is demonstrated for the synthesis of three different Affibody molecules. The cysteine residue introduced at the site of ligation can be used for directed immobilization and does not interfere with the function of the investigated proteins. This strategy combines a high-yield production method with facilitated preparation of proteins with different C-terminal modifications.
authors.affiliation: KTH Royal Institute of Technology, School of Biotechnology, Division of Molecular Biotechnology, AlbaNova University Center, 10691 Stockholm, Sweden.
authors.forename: Joel
authors.initials: J
authors.lastname: Lindgren
authors.forename: Caroline
authors.initials: C
authors.lastname: Ekblad
authors.forename: Lars
authors.initials: L
authors.lastname: Abrahmsén
authors.forename: Amelie
authors.initials: A
authors.lastname: Eriksson Karlström
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry* / Q000378:metabolism; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D020650:Combinatorial Chemistry Techniques; D006801:Humans; D055767:Immobilized Proteins / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D015202:Protein Engineering
chemical_list: D000906:Antibodies; D055767:Immobilized Proteins; D008024:Ligands; D010446:Peptide Fragments