title: [Diagnostic assessment of peritoneal fluid cytology in horses with abdominal neoplasia].
abstract: OBJECTIVE
  To evaluate the diagnostic value of peritoneal fluid (PF) cytology for clinical diagnosis of abdominal neoplasia in horses.
  
  
  METHODS
  Ten horses with histopathologically confirmed abdominal neoplasia, in which a PF analysis was performed, were included in this retrospective study. PF was analyzed for total protein concentration and a nucleated cell count was performed. Using cytological criteria of malignancy, the PF samples were evaluated regarding their probability of malignancy.
  
  
  RESULTS
  Cytologic classification of cells according to criteria of malignancy allowed a positive cytologic diagnosis of neoplasia in 5 out of 10 peritoneal fluid samples. Malignant lymphoma was the most commonly diagnosed neoplasia (3/10) and could be identified by cytology in 2/3 cases. In 1/2 horses with plasma cell myeloma neoplastic cells were similarly found. Malignant melanoma (2/10) was diagnosed using cytology in one case (presence of melanin-containing cells). Cytological diagnosis of malignant neoplasia was established in the only horse with gastric squamous cell carcinoma, but the morphology of the identified tumour cells did not allow a specific diagnosis. Thus, a definitive diagnosis was achieved in 4/5 horses with proven abdominal neoplasia. The horses with adenocarcinoma (1/10) and haemangiosarcoma (1/10) had no evidence of neoplasia based on cytological findings. No relationship between total protein concentration or the nucleated cell count with the histolopathological diagnosis of abdominal neoplasia was found. Abnormal mitotic figures were considered of greater diagnostic value than the overall mitotic rate.
  
  
  CONCLUSIONS
  The implementation of nuclear criteria of malignancy in the cytologic evaluation of PF samples allows the identification of neoplastic cells to an acceptable degree. For this purpose, the knowledge of the highly variable morphological features of mesothelial cells is essential. The absence of malignant cells does not rule out abdominal neoplasia.
  
  
  CONCLUSIONS
  PF cytology should be considered as a valuable, minimally invasive, simple, and rapid diagnostic technique in horses with suspected abdominal neoplasia.
authors.affiliation: Medizinische Tierklinik, der Universität Leipzig, Leipzig. recknagel@vetmed.uni-leipzig.de
authors.forename: S
authors.initials: S
authors.lastname: Recknagel
authors.forename: M
authors.initials: M
authors.lastname: Nicke
authors.forename: G F
authors.initials: GF
authors.lastname: Schusser
mesh_terms: D000008:Abdominal Neoplasms / Q000175:diagnosis / Q000662:veterinary*; D000230:Adenocarcinoma / Q000175:diagnosis / Q000662:veterinary; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000662:veterinary; D004379:Duodenal Neoplasms / Q000175:diagnosis / Q000662:veterinary; D006394:Hemangiosarcoma / Q000175:diagnosis / Q000662:veterinary; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D008223:Lymphoma / Q000175:diagnosis / Q000662:veterinary; D008545:Melanoma / Q000175:diagnosis / Q000662:veterinary; D008643:Mesentery; D009101:Multiple Myeloma / Q000175:diagnosis / Q000662:veterinary; D010534:Peritoneal Neoplasms / Q000175:diagnosis / Q000662:veterinary; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000662:veterinary