title: Rapid spoligotyping of Mycobacterium tuberculosis complex bacteria by use of a microarray system with automatic data processing and assignment.
abstract: Membrane-based spoligotyping has been converted to DNA microarray format to qualify it for high-throughput testing. We have shown the assay's validity and suitability for direct typing from tissue and detecting new spoligotypes. Advantages of the microarray methodology include rapidity, ease of operation, automatic data processing, and affordability.
authors.affiliation: Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Molecular Pathogenesis, Jena, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Ruettger
authors.forename: Johanna
authors.initials: J
authors.lastname: Nieter
authors.forename: Artem
authors.initials: A
authors.lastname: Skrypnyk
authors.forename: Ines
authors.initials: I
authors.lastname: Engelmann
authors.forename: Albrecht
authors.initials: A
authors.lastname: Ziegler
authors.forename: Irmgard
authors.initials: I
authors.lastname: Moser
authors.forename: Stefan
authors.initials: S
authors.lastname: Monecke
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
authors.forename: Konrad
authors.initials: K
authors.lastname: Sachse
mesh_terms: D001330:Electronic Data Processing / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013997:Time Factors