title: Novel bioassay demonstrates attraction of the white potato cyst nematode Globodera pallida (Stone) to non-volatile and volatile host plant cues.
abstract: Potato cyst nematodes (PCNs) are a major pest of solanaceous crops such as potatoes, tomatoes, and eggplants and have been widely studied over the last 30 years, with the majority of earlier studies focusing on the identification of natural hatching factors. As a novel approach, we focused instead on chemicals involved in nematode orientation towards its host plant. A new dual choice sand bioassay was designed to study nematode responses to potato root exudates (PRE). This bioassay, conducted together with a traditional hatching bioassay, showed that biologically active compounds that induce both hatching and attraction of PCNs can be collected by water extraction of incised potato roots. Furthermore, our results demonstrated that PCN also were attracted by potato root volatiles. Further work is needed to fully understand how PCNs use host plant chemical cues to orientate towards hosts. Nevertheless, the simple attraction assay used in this study provides an important tool for the identification of host-emitted attractants.
authors.affiliation: Department of Plant Protection Biology, Swedish University of Agricultural Sciences, 230 53, Alnarp, Sweden. kfarnier@students.latrobe.edu.au
authors.forename: Kevin
authors.initials: K
authors.lastname: Farnier
authors.forename: Marie
authors.initials: M
authors.lastname: Bengtsson
authors.forename: Paul G
authors.initials: PG
authors.lastname: Becher
authors.forename: Johanna
authors.initials: J
authors.lastname: Witzell
authors.forename: Peter
authors.initials: P
authors.lastname: Witzgall
authors.forename: Sanja
authors.initials: S
authors.lastname: Manduríc
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D054884:Host-Pathogen Interactions; D009348:Nematoda / Q000254:growth & development / Q000502:physiology*; D018517:Plant Roots / Q000378:metabolism / Q000469:parasitology*; D011198:Solanum tuberosum / Q000378:metabolism / Q000469:parasitology*; D055549:Volatile Organic Compounds / Q000378:metabolism
chemical_list: D055549:Volatile Organic Compounds