title: A pantropic canine coronavirus genetically related to the prototype isolate CB/05.
abstract: We report the genetic and biological characterisation of a novel pantropic canine coronavirus (CCoV), strain 450/07, which caused the death of a 60-day-old miniature pinscher. At the genetic level, this virus was strictly related to the prototype strain CB/05, but displayed some unique features. After experimental infection with the new pantropic isolate, most inoculated dogs showed diarrhoea and acute lymphopenia. Gross lesions and histological changes were mainly evident in the gut and lymphoid tissues, although some animals showed remarkable changed also in parenchymatous organs. The viral RNA was detected in the faeces and/or internal organs of most pups. These findings seem to indicate that strain 450/07 is able to spread to internal organs (mainly lymphoid tissues), causing lymphopenia but inducing a mild disease.
authors.affiliation: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Valenzano, Bari, Italy. n.decaro@veterinaria.uniba.it
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Viviana
authors.initials: V
authors.lastname: Mari
authors.forename: Marcela
authors.initials: M
authors.lastname: von Reitzenstein
authors.forename: Maria Stella
authors.initials: MS
authors.lastname: Lucente
authors.forename: Francesco
authors.initials: F
authors.lastname: Cirone
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Vickie L
authors.initials: VL
authors.lastname: King
authors.forename: Antonio
authors.initials: A
authors.lastname: Di Bello
authors.forename: Katia
authors.initials: K
authors.lastname: Varello
authors.forename: Shucheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Maria
authors.initials: M
authors.lastname: Caramelli
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D018352:Coronavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D004283:Dog Diseases / Q000473:pathology / Q000821:virology; D004285:Dogs; D005243:Feces / Q000821:virology; D008231:Lymphopenia / Q000473:pathology / Q000662:veterinary / Q000821:virology; D019590:Nucleocapsid Proteins / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D014763:Viral Matrix Proteins / Q000235:genetics
chemical_list: D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D014763:Viral Matrix Proteins