title: Enterococcus ureilyticus sp. nov. and Enterococcus rotai sp. nov., two urease-producing enterococci from the environment.
abstract: A set of 25 urease-producing, yellow-pigmented enterococci was isolated from environmental sources. Phenotypic classification divided the isolates into two phena. Both phena were characterized using 16S rRNA gene sequence analysis, DNA base composition, rep-PCR fingerprinting and automated ribotyping. The obtained data distinguished the isolates from all members of the genus Enterococcus with validly published names and placed them in the Enterococcus faecalis species group. DNA-DNA hybridization experiments, pheS and rpoA sequencing and whole-cell protein electrophoresis provided conclusive evidence for the classification of each phenon as a novel species of the genus Enterococcus, for which the names Enterococcus ureilyticus sp. nov. (type strain CCM 4629(T)  = LMG 26676(T)  = CCUG 48799(T)), inhabiting water and plants, and Enterococcus rotai sp. nov. (type strain CCM 4630(T)  = LMG 26678(T)  = CCUG 61593(T)), inhabiting water, insects (mosquitoes) and plants, are proposed.
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Ivo
authors.initials: I
authors.lastname: Sedláček
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Pavla
authors.initials: P
authors.lastname: Holochová
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Ivana
authors.initials: I
authors.lastname: Mašlaňová
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Marcel
authors.initials: M
authors.lastname: Kosina
authors.affiliation: DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Bryndová
authors.affiliation: Laboratory of Microbiology, Faculty of Sciences, Gent University, B-9000 Gent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.affiliation: Institute of Vertebrate Biology, Academy of Sciences CR, v.v.i., Květná 8, 603 65 Brno, Czech Republic.
authors.forename: Ivo
authors.initials: I
authors.lastname: Rudolf
authors.affiliation: Institute of Vertebrate Biology, Academy of Sciences CR, v.v.i., Květná 8, 603 65 Brno, Czech Republic.
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Hubálek
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Švec
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics; D060766:Drinking Water / Q000382:microbiology; D016983:Enterococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004783:Environmental Microbiology; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014510:Urease / Q000096:biosynthesis*
chemical_list: D004269:DNA, Bacterial; D060766:Drinking Water; D012336:RNA, Ribosomal, 16S; D014510:Urease