title: DNA binding studies of Vinca alkaloids: experimental and computational evidence.
abstract: Fluorescence studies on the indole alkaloids vinblastine sulfate, vincristine sulfate, vincamine and catharanthine have demonstrated the DNA binding ability of these molecules. The binding mode of these molecules in the minor groove of DNA is non-specific. A new parameter of the purine-pyrimidine base sequence specificty was observed in order to define the non-specific DNA binding of ligands. Catharanthine had shown 'same' pattern of 'Pu-Py' specificity while evaluating its DNA binding profile. The proton resonances of a DNA decamer duplex were assigned. The models of the drug:DNA complexes were analyzed for DNA binding features. The effect of temperature on the DNA binding was also evaluated.
authors.affiliation: Department of Chemistry, Faculty of Engineering, Dayalbagh Educational Institute, Dayalbagh, Agra-282 110, India.
authors.forename: Prateek
authors.initials: P
authors.lastname: Pandya
authors.forename: Surendra P
authors.initials: SP
authors.lastname: Gupta
authors.forename: Kumud
authors.initials: K
authors.lastname: Pandav
authors.forename: Ritu
authors.initials: R
authors.lastname: Barthwal
authors.forename: B
authors.initials: B
authors.lastname: Jayaram
authors.forename: Surat
authors.initials: S
authors.lastname: Kumar
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D011687:Purines / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry; D031053:Vinca / Q000737:chemistry; D014748:Vinca Alkaloids / Q000737:chemistry*
chemical_list: D011687:Purines; D011743:Pyrimidines; D014748:Vinca Alkaloids; D004247:DNA