title: [The molecular mechanisms of resistance to B-lactams of pathogens of hospital-acquired infections].
abstract: The data of local microbiologic monitoring was used to study the profiles and mechanisms of resistance to beta-lactams antibiotics of gram-negative isolates of bacteria, pathogens of hospital-acquired infections in hospital reanimation and surgical departments. The study included 210 clinical isolates of pathogens of hospital-acquired infections: Pseudomonas aeruginosa--86 (40.9%), Acinetobacter baumannii--45 (21.4%), Klebsiella pneumoniae--52 (24.8%), Escherichia coli-23 (11.0%), Enterobacter spp.--4 (1.9%). The profiles of resistance to antibiotics were analyzed using the technique of serial micro-dilutions. The detection of the most common and clinically significant gens of beta-lactamases of gram-negative bacteria was implemented using the PCR technique and sequence analysis. The most activity was detected among carbapenems and cephaperazone/sulbactam. The local characteristics of prevalence of gens coding beta-lactamases (TEM, SHV, CTX) in K. pneumoniae and E. coli were established The study detected 11 isolates of P aeruginosa resistant to carbapenems and having genetic determinants of VIM-group and coding metal-beta-lactamases. The discussed data permits to assess the indicators of resistance to beta-lactams antibiotics and basic mechanisms of resistance of causative agents of hospital-acquired infections. The studies of this kind are unique for every type of hospital-acquired infection. The results can be used in the development of the concept of etiotropic and empiric therapy.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Mironov
authors.forename: I V
authors.initials: IV
authors.lastname: Krapivina
authors.forename: D E
authors.initials: DE
authors.lastname: Mudrak
authors.forename: D V
authors.initials: DV
authors.lastname: Ivanov
mesh_terms: D000900:Anti-Bacterial Agents; D001419:Bacteria / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D001424:Bacterial Infections / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D018550:Cephalosporin Resistance / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D007049:Iatrogenic Disease; D008826:Microbial Sensitivity Tests / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001618:beta-Lactamases