title: Systems biotechnology of animal cells: the road to prediction.
abstract: A central concern of biopharmaceutical R&D is the production of sufficient quantities of recombinant products from manufacturing processes based on animal cell culture. The way in which bioprocess researchers have addressed this question experienced a tremendous shift over the years, progressing from almost empirical to more rational approaches. A step further is the application of systems biotechnology: recent technological advances for large-scale cell state characterization and creative methods for host cell modeling are becoming crucial for next-generation bioprocess optimization. Here we provide an overview of the main trends towards this goal, with a focus on metabolic models as central scaffolds for data integration and prediction of bioprocess outcomes.
authors.affiliation: Instituto de Tecnologia Química e Biológica-Universidade Nova de Lisboa, 2780-901 Oeiras, Portugal.
authors.forename: Nuno
authors.initials: N
authors.lastname: Carinhas
authors.forename: Rui
authors.initials: R
authors.lastname: Oliveira
authors.forename: Paula Marques
authors.initials: PM
authors.lastname: Alves
authors.forename: Manuel J T
authors.initials: MJ
authors.lastname: Carrondo
authors.forename: Ana P
authors.initials: AP
authors.lastname: Teixeira
mesh_terms: D000818:Animals; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D023281:Genomics / Q000379:methods*; D049490:Systems Biology / Q000379:methods*; D013678:Technology, Pharmaceutical