title: Isolation and characterization of 10 SSR markers of Momordica charantia (Cucurbitaceae).
abstract: OBJECTIVE
  Microsatellite markers were isolated and characterized from the genome of Momordica charantia (bitter melon) to be applied in studies of genetic diversity and population structure.
  
  
  RESULTS
  Twenty-five microsatellite loci were isolated from the genome of bitter melon using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) method. Ten loci were polymorphic, and the number of alleles per locus ranged from three to seven, with the observed heterozygosity ranging from 0.46 to 0.65. The markers also amplified successfully in the related species M. cochinchinensis and Cucurbita pepo.
  
  
  CONCLUSIONS
  These markers will have potential utility for applications in genetic diversity evaluation, molecular fingerprinting, identification, comparative genomics analysis, and genetic mapping in Momordica species, as well as in C. pepo.
authors.affiliation: College of Forestry, Henan University of Science and Technology, Luoyang, People's Republic of China. guodalong@mail.haust.edu.cn
authors.forename: Da-Long
authors.initials: DL
authors.lastname: Guo
authors.forename: Ju-Ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Xue
authors.forename: Xiao-Gai
authors.initials: XG
authors.lastname: Hou
mesh_terms: D017931:DNA Primers / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D029777:Momordica charantia / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant