title: Atomic structure of the nuclear pore complex targeting domain of a Nup116 homologue from the yeast, Candida glabrata.
abstract: The nuclear pore complex (NPC), embedded in the nuclear envelope, is a large, dynamic molecular assembly that facilitates exchange of macromolecules between the nucleus and the cytoplasm. The yeast NPC is an eightfold symmetric annular structure composed of ~456 polypeptide chains contributed by ~30 distinct proteins termed nucleoporins. Nup116, identified only in fungi, plays a central role in both protein import and mRNA export through the NPC. Nup116 is a modular protein with N-terminal "FG" repeats containing a Gle2p-binding sequence motif and a NPC targeting domain at its C-terminus. We report the crystal structure of the NPC targeting domain of Candida glabrata Nup116, consisting of residues 882-1034 [CgNup116(882-1034)], at 1.94 Å resolution. The X-ray structure of CgNup116(882-1034) is consistent with the molecular envelope determined in solution by small-angle X-ray scattering. Structural similarities of CgNup116(882-1034) with homologous domains from Saccharomyces cerevisiae Nup116, S. cerevisiae Nup145N, and human Nup98 are discussed.
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA. psampath@aecom.yu.edu
authors.forename: Parthasarathy
authors.initials: P
authors.lastname: Sampathkumar
authors.forename: Seung Joong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Danalyn
authors.initials: D
authors.lastname: Manglicmot
authors.forename: Kevin T
authors.initials: KT
authors.lastname: Bain
authors.forename: Jeremiah
authors.initials: J
authors.lastname: Gilmore
authors.forename: Tarun
authors.initials: T
authors.lastname: Gheyi
authors.forename: Jeremy
authors.initials: J
authors.lastname: Phillips
authors.forename: Ursula
authors.initials: U
authors.lastname: Pieper
authors.forename: Javier
authors.initials: J
authors.lastname: Fernandez-Martinez
authors.forename: Josef D
authors.initials: JD
authors.lastname: Franke
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Matsui
authors.forename: Hiro
authors.initials: H
authors.lastname: Tsuruta
authors.forename: Shane
authors.initials: S
authors.lastname: Atwell
authors.forename: Devon A
authors.initials: DA
authors.lastname: Thompson
authors.forename: J Spencer
authors.initials: JS
authors.lastname: Emtage
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Wasserman
authors.forename: Michael P
authors.initials: MP
authors.lastname: Rout
authors.forename: Andrej
authors.initials: A
authors.lastname: Sali
authors.forename: J Michael
authors.initials: JM
authors.lastname: Sauder
authors.forename: Steven C
authors.initials: SC
authors.lastname: Almo
authors.forename: Stephen K
authors.initials: SK
authors.lastname: Burley
mesh_terms: D000595:Amino Acid Sequence; D041221:Candida glabrata / Q000737:chemistry; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000737:chemistry; D009685:Nuclear Envelope / Q000737:chemistry; D022022:Nuclear Pore / Q000737:chemistry*; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D005656:Fungal Proteins; D046912:Multiprotein Complexes; C077730:NUP116 protein, S cerevisiae; D028861:Nuclear Pore Complex Proteins; C465451:Nup98 protein, human; D029701:Saccharomyces cerevisiae Proteins