title: Therapeutic efficacy of an antibiotic-loaded nanosheet in a murine burn-wound infection model.
abstract: Polymeric ultra-thin films (nanosheets) possess unique properties that make them suitable materials for various biomedical applications. In our previous study, we assessed the use of an antibiotic (tetracycline, TC)-loaded nanosheet (or "TC-nanosheet") for the treatment of gastrointestinal tissue defects. The nanosheet consisted of three functional layers: layer-by-layer nanosheet as a stable platform, TC as an antimicrobial agent with autofluorescence for tracing, and a poly(vinyl acetate) nanosheet to act as a protecting layer. The TC-nanosheet has high flexibility, adhesive strength and transparency. Here, we evaluated the effectiveness of the TC-nanosheet in preventing full thickness burn-wound infections. In an in vivo study, murine dorsal skin was injured by full-thickness burns and then infected with Pseudomonas aeruginosa (P. aeruginosa), a common bacterium causing burn-associated infections. The wound site was treated either with a TC-nanosheet, TC-unloaded nanosheet or left untreated. Wound management was facilitated by the high transparency of the TC-nanosheet. The TC-nanosheet significantly improved burn-wound infection by P. aeruginosa in mice. Indeed, all mice treated with the TC-nanosheet survived, whereas the other treatment groups displayed increased rates of mortality due to bacterial infection. According to histological analyses and viable bacterial counting in the liver (bacterial translocation), the TC-nanosheets were able to prevent not only the local inflammation but also systemic inflammation. We conclude that the TC-nanosheet can act as an effective treatment for full-thickness burn-wound infection. Hence, the TC-nanosheet is a promising therapeutic tool for burn-wound management in severely burn-injured patients.
authors.affiliation: Department of Life Science and Medical BioScience, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo 162-8480, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Saito
authors.forename: Hiromi
authors.initials: H
authors.lastname: Miyazaki
authors.forename: Toshinori
authors.initials: T
authors.lastname: Fujie
authors.forename: Shinya
authors.initials: S
authors.lastname: Ohtsubo
authors.forename: Manabu
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Daizoh
authors.initials: D
authors.lastname: Saitoh
authors.forename: Shinji
authors.initials: S
authors.lastname: Takeoka
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002056:Burns / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D008099:Liver / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D050296:Microbial Viability / Q000187:drug effects; D053758:Nanoparticles / Q000627:therapeutic use* / Q000648:ultrastructure; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013752:Tetracycline / Q000494:pharmacology / Q000627:therapeutic use*; D016896:Treatment Outcome; D014946:Wound Infection / Q000150:complications / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline