title: A bi-cistronic baculovirus expression vector for improved recombinant protein production.
abstract: Baculoviruses are one of the most studied insect viruses both in basic virology research and in biotechnology applications. Incorporating an internal ribosome entry site (IRES) into the baculovirus genome generates bi-cistronic baculoviruses expression vectors that produce two genes of interest. The bi-cistronic baculoviruses also facilitate recombinant virus isolation and titer determination when the green fluorescent protein was co-expressed. Furthermore, when the secretion proteins were co-expressed with the cytosolic green fluorescent protein, the cell lysis and cytosolic protein released into the culture medium could be monitored by the green fluorescence, thus facilitating purification of the secreted proteins.
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, Chungli, Taiwan. tywu@cycu.edu.tw
authors.forename: Tzong-Yuan
authors.initials: TY
authors.lastname: Wu
authors.forename: Ying-Ju
authors.initials: YJ
authors.lastname: Chen
authors.forename: Chao-Yi
authors.initials: CY
authors.lastname: Teng
authors.forename: Wen-Shuo
authors.initials: WS
authors.lastname: Chen
authors.forename: Oliver
authors.initials: O
authors.lastname: Villaflores
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D018411:Spodoptera / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins