title: Enhanced cell uptake of superparamagnetic iron oxide nanoparticles through direct chemisorption of FITC-Tat-PEG₆₀₀-b-poly(glycerol monoacrylate).
abstract: Magnetic nanoparticles (MNPs) functionalized with specific ligands are emerging as a highly integrated platform for cancer targeting, drug delivery, and magnetic resonance imaging applications. In this study, we describe a multifunctional magnetic nanoparticle system (FITC-Tat MNPs) consisting of a fluorescently labeled cell penetrating peptide (FITC-Tat peptide), a biocompatible block copolymer PEG(600)-b-poly(glycerol monoacrylate) (PEG(600)-b-PGA), and a superparamagnetic iron oxide (SPIO) nanoparticle core. The particles were prepared by direct chemisorption of PEG(600)-b-PGA conjugated with FITC-Tat peptide on the SPIO nanoparticles. FITC-MNPs without Tat were prepared for comparison. Flow cytometry assays revealed significantly higher uptake of FITC-Tat MNPs compared to FITC-MNPs in Caco-2 cells. These results were confirmed using confocal laser scanning microscopy (LSCM), which further demonstrated that the FITC-Tat MNPs accumulated in the cytoplasm and nucleus while the FITC-MNPs were localized in the cell membrane compartments. The FITC-Tat MNPs did not exhibit observable cytotoxicity in MTS assays.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Chenhong
authors.initials: C
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Qiao
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Husheng
authors.initials: H
authors.lastname: Yan
authors.forename: Keliang
authors.initials: K
authors.lastname: Liu
mesh_terms: D000179:Acrylates / Q000737:chemistry* / Q000633:toxicity; D001692:Biological Transport; D018938:Caco-2 Cells; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D005990:Glycerol / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D007700:Kinetics; D058185:Magnetite Nanoparticles; D018613:Microscopy, Confocal; D010316:Particle Size; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000633:toxicity; D011108:Polymers / Q000737:chemistry* / Q000633:toxicity; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity
chemical_list: D000179:Acrylates; D058185:Magnetite Nanoparticles; D010446:Peptide Fragments; D011108:Polymers; D054322:tat Gene Products, Human Immunodeficiency Virus; C043941:polyglycerol; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate; D005990:Glycerol