title: [siRNA-induced down-regulation of Livin expression increases spontaneous apoptosis in K562 cell line].
abstract: This study was aimed to observe the effects of siRNA on Livin expression and function in K562 cells. Livin siRNA were designed and synthesized, then were transfected into K562 cells by using AMAXA nucle transfactor. Expressions of Livin mRNA and protein in transfected K562 cells was detected by RT-PCR and Western blot respectively. Non-transfected cells were used as control. The enhanced green fluorescent protein plasmid was used as positive control and the transfection efficiency was detected by flow cytometry. Cell apoptosis was measured by flow cytometry with Annexin V-FITC/PI double staining. The results showed that the transfection efficiency of electroporation method was about 50. The synthesized siRNA inhibited livin expression at both mRNA and protein levels. The rate of K562 cell apoptosis increased from (9.63 ± 0.89) in control group to (12.07 ± 1.39) and (27.41 ± 2.30) at 24 h and 48 h after transfection, respectively (P < 0.05). It is concluded that the siRNA can inhibit anti-apoptosis of livin gene via down-regulating livin gene expression, which may provide the new method for anti-leukemia study.
authors.affiliation: Department of Hematology, Linyi Cancer Hospital, Shandong Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lü
authors.forename: Zhi-Chao
authors.initials: ZC
authors.lastname: Chen
authors.forename: Qiu-Bai
authors.initials: QB
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.forename: Yong
authors.initials: Y
authors.lastname: You
authors.forename: Ping
authors.initials: P
authors.lastname: Zou
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D020014:K562 Cells; D009363:Neoplasm Proteins / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C419866:BIRC7 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering