title: Comparison of cytotoxicity of pristine and covalently functionalized multi-walled carbon nanotubes in RAW 264.7 macrophages.
abstract: Carbon nanotubes may be applied in different fields including biomedicine and mechanical engineering. It is important to understand the potential hazards of carbon nanotubes. In the present study, the toxicological effects of the pristine multi-walled carbon nanotubes (p-MWCNTs) and taurine functionalized multi-walled carbon nanotubes (tau-MWCNTs) were assessed on RAW 264.7 macrophages. We tested cell viability, GSH/GSSG ratio, apoptosis, intracellular calcium concentration, ultrastructural changes of cell morphology, and the release of IL-8. We observed the loss of cell viability, decline in the cellular GSH/GSSG ratio, increase of IL-8, and the increase of intracellular calcium concentration in RAW 264.7 macrophages when exposed to p-MWCNTs at high dosage. Additionally, exposure to p-MWCNTs resulted in ultrastructural and morphological changes in RAW 264.7 macrophages. In contrast, the RAW 264.7 macrophages exposed to the tau-MWCNTs did not exhibit altered morphology. Our results conclude that the tau-MWCNTs show lower toxicity than that of p-MWCNTs.
authors.affiliation: Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Luo
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Deng
authors.forename: Xizhong
authors.initials: X
authors.lastname: Shen
authors.forename: Ling
authors.initials: L
authors.lastname: Dong
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000473:pathology*; D008422:Materials Testing; D051379:Mice; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000633:toxicity* / Q000648:ultrastructure
chemical_list: D037742:Nanotubes, Carbon