title: Altered subcellular localization of transcription factor TEAD4 regulates first mammalian cell lineage commitment.
abstract: In the preimplantation mouse embryo, TEAD4 is critical to establishing the trophectoderm (TE)-specific transcriptional program and segregating TE from the inner cell mass (ICM). However, TEAD4 is expressed in the TE and the ICM. Thus, differential function of TEAD4 rather than expression itself regulates specification of the first two cell lineages. We used ChIP sequencing to define genomewide TEAD4 target genes and asked how transcription of TEAD4 target genes is specifically maintained in the TE. Our analyses revealed an evolutionarily conserved mechanism, in which lack of nuclear localization of TEAD4 impairs the TE-specific transcriptional program in inner blastomeres, thereby allowing their maturation toward the ICM lineage. Restoration of TEAD4 nuclear localization maintains the TE-specific transcriptional program in the inner blastomeres and prevents segregation of the TE and ICM lineages and blastocyst formation. We propose that altered subcellular localization of TEAD4 in blastomeres dictates first mammalian cell fate specification.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA.
authors.forename: Pratik
authors.initials: P
authors.lastname: Home
authors.forename: Biswarup
authors.initials: B
authors.lastname: Saha
authors.forename: Soma
authors.initials: S
authors.lastname: Ray
authors.forename: Debasree
authors.initials: D
authors.lastname: Dutta
authors.forename: Sumedha
authors.initials: S
authors.lastname: Gunewardena
authors.forename: Byunggil
authors.initials: B
authors.lastname: Yoo
authors.forename: Arindam
authors.initials: A
authors.lastname: Pal
authors.forename: Jay L
authors.initials: JL
authors.lastname: Vivian
authors.forename: Melissa
authors.initials: M
authors.lastname: Larson
authors.forename: Margaret
authors.initials: M
authors.lastname: Petroff
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Gallagher
authors.forename: Vincent P
authors.initials: VP
authors.lastname: Schulz
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: White
authors.forename: Thaddeus G
authors.initials: TG
authors.lastname: Golos
authors.forename: Barry
authors.initials: B
authors.lastname: Behr
authors.forename: Soumen
authors.initials: S
authors.lastname: Paul
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D053624:Blastocyst Inner Cell Mass / Q000166:cytology / Q000378:metabolism; D001757:Blastomeres / Q000166:cytology / Q000378:metabolism; D015153:Blotting, Western; D000071616:CDX2 Transcription Factor; D002417:Cattle; D019070:Cell Lineage; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008253:Macaca mulatta; D051379:Mice; D008822:Mice, Transgenic; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000091262:TEA Domain Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000071616:CDX2 Transcription Factor; C487514:Cdx2 protein, mouse; D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D018398:Homeodomain Proteins; D009124:Muscle Proteins; D000091262:TEA Domain Transcription Factors; C101204:Tead4 protein, mouse; D014157:Transcription Factors; D049452:Green Fluorescent Proteins