title: Immunoexpression of claudin-1 and Nm23-H1 in metastatic and nonmetastatic lower lip squamous-cell carcinoma.
abstract: The aim of this study was to evaluate the immunoexpression of claudin-1 and Nm23-H1 in metastatic and nonmetastatic lower lip squamous-cell carcinoma (LLSCC). Twenty LLSCCs with regional nodal metastasis and 20 LLSCCs without metastases were selected. The percentage of claudin-1 staining and the staining intensity and percentage of Nm23-H1 staining in each tumor core were assessed. Metastatic tumors exhibited higher expression of claudin-1 than nonmetastatic tumors (P = 0.030). Similarly, stage III and IV LLSCCs showed higher expression of claudin-1 than stages I and II (P = 0.026). The percentage of claudin-1 staining was scored as 2 in most well-differentiated and moderately differentiated tumors, whereas poorly differentiated tumors showed a relatively similar distribution of scores 2, 1, and 0 (P = 0.648). Regarding Nm23-H1, there was a predominance of negative cases for both metastatic and nonmetastatic tumors (P = 0.235). In addition, no significant differences in the percentage of Nm23-H1-negative and Nm23-H1-positive cases were observed regarding the clinical staging (P = 0.430) and the histologic grading of malignancy (P = 0.702). The results of this study suggest an important role of claudin-1 in the development of metastasis in LLSCCs. In contrast, the present findings do not support a significant role of Nm23-H1 in metastasis suppression of LLSCC.
authors.affiliation: Oral Pathology Department, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Ana Rafaela Luz
authors.initials: AR
authors.lastname: de Aquino
authors.forename: Cyntia Helena Pereira
authors.initials: CH
authors.lastname: de Carvalho
authors.forename: Cassiano Francisco Weege
authors.initials: CF
authors.lastname: Nonaka
authors.forename: Roseana de Almeida
authors.initials: Rde A
authors.lastname: Freitas
authors.forename: Lélia Batista
authors.initials: LB
authors.lastname: de Souza
authors.forename: Leão Pereira
authors.initials: LP
authors.lastname: Pinto
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D062445:Claudin-1 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008048:Lip Neoplasms / Q000378:metabolism* / Q000473:pathology; D054778:NM23 Nucleoside Diphosphate Kinases / Q000378:metabolism*; D009362:Neoplasm Metastasis
chemical_list: C568108:CLDN1 protein, human; D062445:Claudin-1; D054778:NM23 Nucleoside Diphosphate Kinases