title: Genetic characterization of a pigeon paramyxovirus type 1 isolated from Columba livia in Uruguay.
abstract: SUMMARY. The isolation and molecular characterization of pigeon paramyxovirus type 1 (PPMV-1) from a sick racing pigeon in Uruguay is reported for the first time. Hemagglutination inhibition (HI) tests were performed to detect antibodies against avian paramyxovirus serotype 1 (APMV-1), and a HI titer of 1/32 was obtained. Tracheal and cloacal swabs were processed by real-time reverse transcription-polymerase chain reaction (rRT-PCR) with the use of the National Veterinary Services Laboratory-U.S. Department of Agriculture validated matrix (M) gene assay and were positive for APMV-1. Viral isolation in embryonated chicken eggs confirmed the molecular detection of the isolate. A fragment corresponding to the 3' region of the fusion (F) protein gene was amplified by RT-PCR, and subsequently sequenced. The deduced amino acid sequence at the F protein cleavage site displayed the motif 112RRQKR/F117. Phylogenetic analysis of this part of the genome allowed the isolated virus to be grouped in the lineage VIb/ 4b, which suggests that it shares the same ecologic niche with other PPMV-1 that were found in the region, and it is not imported as other European or North American viruses.
authors.affiliation: Departamento Virologia, DILAVE "M. C. Rubino", DGSG-MGAP, Ruta 8, km 17.5, C.P. 12100, Montevideo, Uruguay. racastro@mgap.gub.uy
authors.forename: E R
authors.initials: ER
authors.lastname: Castro
authors.forename: F
authors.initials: F
authors.lastname: Zanetti
authors.forename: J
authors.initials: J
authors.lastname: Arbiza
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D010856:Columbidae; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D014583:Uruguay; D014760:Viral Fusion Proteins / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D014760:Viral Fusion Proteins