title: Human adipose stem cell-conditioned medium increases survival of Friedreich's ataxia cells submitted to oxidative stress.
abstract: Friedreich's ataxia (FA) is a multisystemic disorder characterized by progressive gait, ataxia, and cardiomyopathy. There are few treatments for this disease; thus, we analyzed in vitro the possible beneficial effect of adult stem cells in FA. To this end, human adipose stem cells from healthy individuals and periodontal ligament cells from FA patients were isolated and cultured. FA cells are especially vulnerable to oxidative stress; thus, they were submitted to this condition and cultured in adipose stem cell-conditioned medium. This resulted in increased cell survival and upregulation of oxidative-stress-related genes as well as frataxin, among other genes. A number of trophic factors were shown to be expressed by the adipose stem cells, especially brain-derived neurotrophic factor (BDNF), which was also identified in the conditioned medium. The culture of the ataxic cells under oxidative stress and in the presence of this trophic factor confirmed its protective effect. Thus, this work demonstrates that adipose stem cell-conditioned medium from healthy individuals is capable of changing the transcription levels of oxidative-stress-related genes in cells that are particularly susceptible to this condition, avoiding cellular degeneration. Also, this work shows how neurotrophic factors, particularly BDNF, are capable of increasing cell survival in response to oxidative stress, which occurs in many neurodegenerative diseases.
authors.affiliation: Neuroscience Institute UMH-CSIC, University Miguel Herna´ndez, San Juan, Spain. jon@umh.es
authors.forename: Jonathan
authors.initials: J
authors.lastname: Jones
authors.forename: Alicia
authors.initials: A
authors.lastname: Estirado
authors.forename: Carolina
authors.initials: C
authors.lastname: Redondo
authors.forename: Carlos
authors.initials: C
authors.lastname: Bueno
authors.forename: Salvador
authors.initials: S
authors.lastname: Martínez
mesh_terms: D000273:Adipose Tissue / Q000473:pathology*; D053687:Adult Stem Cells / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004789:Enzyme Activation; D005621:Friedreich Ataxia / Q000473:pathology*; D015870:Gene Expression; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D010513:Periodontal Ligament / Q000473:pathology; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D017077:Culture Media, Conditioned; D036341:Intercellular Signaling Peptides and Proteins; D016877:Oxidants; D006861:Hydrogen Peroxide; C505407:CASP3 protein, human; D053148:Caspase 3