title: Can dead bacterial cells be defined and are genes expressed after cell death?
abstract: There is a paucity of knowledge on gene expression in dead bacterial cells. Why would this knowledge be useful? The cells are dead. However, the time duration of gene expression following cell death is often unknown, and possibly in the order of minutes. In addition, it is a challenge to determine if bacterial cells are dead, or viable but non-culturable (VBNC), and what is an agreed upon correct definition of dead bacteria. Cells in the bacterial population or community may die at different rates or times and this complicates both the viability and gene expression analysis. In this article, the definition of dead bacterial cells is discussed and its significance in continued gene expression in cells following death. The definition of living and dead has implications for possible, completely, synthetic bacterial cells that may be capable of growth and division.
authors.affiliation: Laboratory of Microbiology, School of Environmental Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1. jtrevors@uoguelph.ca
authors.forename: J T
authors.initials: JT
authors.lastname: Trevors
mesh_terms: D001419:Bacteria / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D050296:Microbial Viability
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media