title: UPLC-MS/MS determination of paeoniflorin, naringin, naringenin and glycyrrhetinic acid in rat plasma and its application to a pharmacokinetic study after oral administration of Si-Ni-San decoction.
abstract: A UPLC-MS/MS method was developed for the simultaneous determination of paeoniflorin, naringin, naringenin and glycyrrhetinic acid in rat plasma. A Waters BEH C(18) column was used with a gradient mobile phase system of methanol-water containing 2 mM ammonium acetate. The analysis was performed on a positive ionization electrospray mass spectrometer via multiple reaction monitoring (MRM). One-step protein precipitation with acetonitrile was used to extract the analytes from plasma. The limits of quantification were 9.800 ng/ml for paeoniflorin, 5.100 ng/ml for naringin, 5.200 ng/ml for naringenin and 10.60 ng/ml for glycyrrhetinic acid, respectively. The intra- and inter-day precision (relative standard deviation, RSD) ranged 4.9-12% and 2.8-13%, respectively. The accuracy (relative error, RE) was from -7.3% to 7.5% at all quality control (QC) levels. The validated method was applied to a pharmacokinetic study in rats after oral administration of Si-Ni-San decoction.
authors.affiliation: Department of Analytical Chemistry, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.forename: Ying
authors.initials: Y
authors.lastname: Qiao
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
authors.forename: Famei
authors.initials: F
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001565:Benzoates / Q000097:blood; D001952:Bridged-Ring Compounds / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D044950:Flavanones / Q000097:blood; D005960:Glucosides / Q000097:blood; D006034:Glycyrrhetinic Acid / Q000097:blood; D057230:Limit of Detection; D008297:Male; D039821:Monoterpenes; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; D006034:Glycyrrhetinic Acid; D039821:Monoterpenes; C510945:shigyaku-san; C015423:peoniflorin; D006027:Glycosides; C005273:naringenin; C005274:naringin