title: Mouse gastrocnemius muscle regeneration after mechanical or cardiotoxin injury.
abstract: The goal of our study was to compare the skeletal muscle regeneration induced by two types of injury: either crushing, that causes muscle degeneration as a result of mechanical devastation of myofibers, or the injection of a cardiotoxin that is a myotoxic agent causing myolysis of myofibers leading to muscle degeneration. Regenerating muscles were analyzed at selected intervals, until the 14th day following the injury. We analyzed their weight and morphology. We also studied the expression of different myosin heavy chain isoforms as a molecular marker of the regeneration progress. Histological analysis revealed that inflammatory response and myotube formation in crushed muscles was delayed compared to cardiotoxin-injected ones. Moreover, the expression of myosin heavy chain isoforms was observed earlier in cardiotoxin-injured versus crushed muscles. We conclude that the dynamics of skeletal muscle regeneration depends on the method of injury.
authors.affiliation: Department of Cytology, Institute of Zoology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
authors.forename: Areta M
authors.initials: AM
authors.lastname: Czerwinska
authors.forename: Wladyslawa
authors.initials: W
authors.lastname: Streminska
authors.forename: Maria A
authors.initials: MA
authors.lastname: Ciemerych
authors.forename: Iwona
authors.initials: I
authors.lastname: Grabowska
mesh_terms: D000818:Animals; D054715:Cardiotoxins / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000254:growth & development / Q000293:injuries / Q000473:pathology; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D009929:Organ Size; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects
chemical_list: D054715:Cardiotoxins; D020033:Protein Isoforms; D012333:RNA, Messenger; D018995:Myosin Heavy Chains