title: Establishing developmental genetics in a self-fertilizing fish (Krytolebias marmoratus).
abstract: Kryptolebias marmoratus is a synchronous hermaphroditic vertebrate that utilizes an ovotestis for reproduction. This fish develops externally, is easy to maintain, and has about a 100-day life cycle, making it a desirable developmental genetic model organism. Here, we present a pilot zygotic mutant screen utilizing the common chemical mutagen, N-ethyl-N-nitrosourea (ENU) to establish genetics in this model species. Selection of clonal stocks and optimal conditions for mutagenizing this fish are presented and the types and frequencies of zygotic mutants are documented in comparison to other fish models. Kryptolebias marmoratus is an exemplar model organism that will complement future developmental genetic screens in vertebrates.
authors.affiliation: Department of Biology, Valdosta State University, Valdosta, GA 31698, USA.
authors.forename: Ginger L
authors.initials: GL
authors.lastname: Moore
authors.forename: Sofia
authors.initials: S
authors.lastname: Sucar
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Newsome
authors.forename: Melissa E
authors.initials: ME
authors.lastname: Ard
authors.forename: Lynda
authors.initials: L
authors.lastname: Bernhardt
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bland
authors.forename: Brian C
authors.initials: BC
authors.lastname: Ring
mesh_terms: D000818:Animals; D001947:Breeding / Q000379:methods*; D003532:Cyprinodontiformes / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D005038:Ethylnitrosourea / Q000494:pharmacology*; D005260:Female; D005820:Genetic Testing / Q000379:methods*; D057850:Hermaphroditic Organisms / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D008297:Male; D016296:Mutagenesis; D015053:Zygote / Q000187:drug effects / Q000254:growth & development
chemical_list: D005038:Ethylnitrosourea