title: Array-comparative genomic hybridization characterization of human pluripotent stem cells.
abstract: During culture adaptation, human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) tend to acquire chromosomal aberrations. Generally, stem cell lines are screened for large-scale chromosomal changes using low resolution karyotype analysis. Recent studies characterizing human stem cells using array-comparative genomic hybridization (aCGH) suggests most abnormalities acquired during culture are under the resolution of karyotype analysis and therefore are routinely missed. Here, we describe a custom-designed stem cell focused microarray utilizing 44K probes, with increased resolution in relevant stem cell-associated and cancer-related genes.
authors.affiliation: Ambry Genetics, Aliso Viejo, CA, USA. aelliott@ambrygen.com
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Elliott
authors.forename: Kristi A Hohenstein
authors.initials: KA
authors.lastname: Elliott
authors.forename: Anja
authors.initials: A
authors.lastname: Kammesheidt
mesh_terms: D055028:Comparative Genomic Hybridization / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D042822:Genomic Instability / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D059785:Karyotype; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism