title: Impact of H-aggregation on activatable MMP-2-specific probes for optical imaging.
abstract: In order to target and image MMP-2 activity using optical imaging, we developed a panel of new MMP-2 probes based on Cy5 and QSY21 as fluorophore/quencher FRET partners, separated by various MMP-2 specific peptide substrates. We compared these probes for their specificity against other MMPs, their rate of activation by MMP-2 and their initial quenching.
authors.affiliation: Département de médecine nucléaire et de radiobiologie, Centre d'imagerie moléculaire de Sherbrooke, Université de Sherbrooke, Sherbrooke, QC, Canada.
authors.forename: Réjean
authors.initials: R
authors.lastname: Lebel
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Bonin
authors.forename: Riadh
authors.initials: R
authors.lastname: Zriba
authors.forename: Adrianna
authors.initials: A
authors.lastname: Radulska
authors.forename: Witold
authors.initials: W
authors.lastname: Neugebauer
authors.forename: Martin
authors.initials: M
authors.lastname: Lepage
mesh_terms: D002232:Carbocyanines; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D006801:Humans; D013058:Mass Spectrometry; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020782:Matrix Metalloproteinases / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes; D010446:Peptide Fragments / Q000378:metabolism*
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D015335:Molecular Probes; D010446:Peptide Fragments; C085321:cyanine dye 5; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2