title: Expression of transforming growth factor-β1 (TGF-β1) and E-cadherin in glioma.
abstract: Gliomas are the most common tumors in the central nervous system. This study aims to investigate the expressions of transforming growth factor-β1 (TGF-β1) and epithelial cadherin (E-cadherin) in human brain glioma tissues and the correlation between their expressions with clinical pathological features and clinical significance. The expressions of mRNA or protein of TGF-β1 and E-cadherin were detected by using reverse transcription polymerase chain reaction (RT-PCR) and Western blot in these tissues. Positive rates of the expression of TGF-β1 and E-cadherin were 62.9 % and 38.6 % in brain tissues of glioma patients. The expressions of mRNA or protein for TGF-β1 in brain glioma tissues were significantly higher than that in normal brain tissues (p < 0.01). Their expressions in well-differentiated glioma brain tissues were lower than those in poorly differentiated glioma brain tissues (p < 0.01). A negative correlation was found between TGF-β1 and E-cadherin in brain glioma tissues (r = -0.302, p < 0.011). The cell numbers of C6 glioma through Transwell chambers were decreased significantly (p < 0.01), and the expression of TGF-β1 was downregulated significantly (p < 0.01). However, the expression of E-cadherin was upregulated significantly (p < 0.01) after transfecting TGF-β1 siRNA. The expression changes of TGF-β1 and E-cadherin may be related to the emergence and the development of glioma. Downregulation of TGF-β1 expression using siRNA can decrease the invasive capability of C6 glioma cells.
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Chuanzong
authors.initials: C
authors.lastname: Deng
authors.forename: Zhikai
authors.initials: Z
authors.lastname: Gu
authors.forename: Yilu
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D002648:Child; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D034622:RNA Interference; D051381:Rats; D014158:Transcription, Genetic; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: D015820:Cadherins; D053773:Transforming Growth Factor beta1