title: New insights into the water-solubilisation of fluorophores by post-synthetic "click" and Sonogashira reactions.
abstract: New synthetic methodologies for the efficient chemical conversion of hydrophobic fluorescent dyes into bioconjugable and water-soluble derivatives are described. The combined use of an original sulfonated terminal alkyne and a metal-mediated reaction, namely the copper-catalysed Huisgen 1,3-dipolar cycloaddition ("click" reaction) or the Sonogashira cross-coupling, is the cornerstone of these novel post-synthetic sulfonation approaches.
authors.affiliation: Université de Rouen, Laboratory COBRA UMR 6014 and FR 3038, IRCOF, 1 Rue Tesnière, 76821 Mont St Aignan Cedex, France.
authors.forename: Cédrik
authors.initials: C
authors.lastname: Massif
authors.forename: Sébastien
authors.initials: S
authors.lastname: Dautrey
authors.forename: Alexandre
authors.initials: A
authors.lastname: Haefele
authors.forename: Raymond
authors.initials: R
authors.lastname: Ziessel
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Renard
authors.forename: Anthony
authors.initials: A
authors.lastname: Romieu
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D014867:Water