title: Urinary recovery of orally administered chromium 51-labeled EDTA, lactulose, rhamnose, d-xylose, 3-O-methyl-d-glucose, and sucrose in healthy adult male Beagles.
abstract: Objective-To provide values for gastrointestinal permeability and absorptive function tests (GIPFTs) with chromium 51 ((51)Cr)-labeled EDTA, lactulose, rhamnose, d-xylose, 3-O-methyl-d-glucose, and sucrose in Beagles and to evaluate potential correlations between markers.   Animals-19 healthy adult male Beagles.   Procedures-A test solution containing 3.7 MBq of (51)Cr-labeled EDTA, 2 g of lactulose, 2 g of rhamnose, 2 g of d-xylose, 1 g of 3-O-methyl-d-glucose, and 8 g of sucrose was administered intragastrically to each dog. Urinary recovery of each probe was determined 6 hours after administration.   Results-Mean ± SD (range) percentage urinary recovery was 6.3 ± 1.6% (4.3% to 9.7%) for (51)Cr-labeled EDTA, 3.3 ± 1.1% (1.7% to 5.3%) for lactulose, 25.5 ± 5.0% (16.7% to 36.9%) for rhamnose, and 58.8% ± 11.0% (40.1% to 87.8%) for 3-O-methyl-d-glucose. Mean (range) recovery ratio was 0.25 ± 0.06 (0.17 to 0.37) for (51)Cr-labeled EDTA to rhamnose, 0.13 ± 0.04 (0.08 to 0.23) for lactulose to rhamnose, and 0.73 ± 0.09 (0.60 to 0.90) for d-xylose to 3-O-methyl-d-glucose. Median (range) percentage urinary recovery was 40.3% (31.6% to 62.7%) for d-xylose and 0% (0% to 0.8%) for sucrose.   Conclusions and Clinical Relevance-Reference values in healthy adult male Beagles for 6 of the most commonly used GIPFT markers were determined. The correlation between results for (51)Cr-labeled EDTA and lactulose was not as prominent as that reported for humans and cats; thus, investigators should be cautious in the use and interpretation of GIPFTs performed with sugar probes in dogs with suspected intestinal dysbiosis.
authors.affiliation: Central Animal Laboratory, University of Turku, Turku, Finland. raffri@utu.fi
authors.forename: Rafael
authors.initials: R
authors.lastname: Frias
authors.forename: Jörg M
authors.initials: JM
authors.lastname: Steiner
authors.forename: David A
authors.initials: DA
authors.lastname: Williams
authors.forename: Satu
authors.initials: S
authors.lastname: Sankari
authors.forename: Elias
authors.initials: E
authors.lastname: Westermarck
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002241:Carbohydrates / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine; D002614:Chelating Agents / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D003938:Diagnostic Techniques, Digestive System; D004285:Dogs / Q000502:physiology*; D004492:Edetic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine; D005772:Gastrointestinal Transit; D007408:Intestinal Absorption; D008297:Male; D010539:Permeability; D012016:Reference Values
chemical_list: D002241:Carbohydrates; D002614:Chelating Agents; D004492:Edetic Acid