title: Assessment of sphingosine-1-phosphate activity in biological samples by receptor internalization and adherens junction formation.
abstract: Sphingosine-1-phosphate (S1P) is a bioactive lipid mediator involved in many biological actions, including vascular homeostasis and immune cell trafficking. S1P activity is mediated by specific G protein-coupled receptors, leading to multiple physiological responses including adherens junction formation in endothelial cells. Here, we describe bioassays for rapidly assessing S1P activity in biological fluids based on ligand-induced receptor internalization in transfected HEK293 cells and consequent adherens junction formation of vascular endothelial cells.
authors.affiliation: Laboratory Medicine, Department of Pathology, Center for Vascular Biology, Weill Cornell Medical College, Cornell University, New York, NY, USA.
authors.forename: Hideru
authors.initials: H
authors.lastname: Obinata
authors.forename: Timothy
authors.initials: T
authors.lastname: Hla
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D057809:HEK293 Cells; D006801:Humans; D008246:Lysophospholipids / Q000737:chemistry / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
chemical_list: D008246:Lysophospholipids; D043562:Receptors, G-Protein-Coupled; C060506:sphingosine 1-phosphate; D013110:Sphingosine