title: Dye labelled monoclonal antibody assay for detection of Toxic Shock Syndrome Toxin -1 from Staphylococcus aureus.
abstract: OBJECTIVE
  The aim of study was to develop a rapid assay, dye labelled monoclonal antibody assay (DLMAA), using non-radioactive organic synthetic dyes for identification of Toxic Shock Syndrome Toxin-1 (TSST-1) producing strains of Staphylococcus aureus.
  
  
  METHODS
  The assay protocol required only two simple steps; addition of TSST-1 antigen to a nitrocellulose membrane and then adding a colloidal dye labelled antibody (D/A) suspension detection reagent.
  
  
  RESULTS
  The sensitivity and specificity of the assay was determined relative to positive and negative strains compared to an ELISA assay. Overall 100% agreement was found between both assays. The sensitivity for detection of TSST-1 was 30 ng.
  
  
  CONCLUSIONS
  The DLMAA did not require handling and disposal of radioactive materials. It is a rapid qualitative technique for detection of TSST-1 toxin at room temperature within a short time.
authors.affiliation: Department of Bacteriology, Guilan University of Medical Sciences, Faculty of Shahid Beheshty, Rasht, Iran.
authors.forename: Khojasteh V
authors.initials: KV
authors.lastname: Javid
authors.forename: Ha
authors.initials: H
authors.lastname: Foster