title: Assessment of a neurophysiological model of the mandibular branch of the facial nerve in rats by electromyography.
abstract: OBJECTIVE
  Our objective was to develop an experimental model for the noninvasive and objective evaluation of facial nerve regeneration in rats using a motor nerve conduction test (electromyography).
  
  
  METHODS
  Twenty-two rats were submitted to neurophysiological evaluation using motor nerve conduction of the mandibular branch of the facial nerve to obtain the compound muscle action potentials (CMAPs). To record the CMAPs, we used two needle electrodes that were inserted into the lower lip muscle of the rat. A supramaximal electrical stimulus was applied, and the values of CMAP latency, amplitude, length, area, and stimulus intensity obtained from each side were compared by use of the Wilcoxon test.
  
  
  RESULTS
  There was no significant difference (all p > 0.05) in latency, amplitude, duration, area, or intensity of stimuli between the two sides. The amplitudes ranged between 1.61 and 8.30 mV, the latencies between 1.03 and 1.97 ms, and the stimulus intensities between 1.50 and 2.90 mA.
  
  
  CONCLUSIONS
  This is a noninvasive, easy, and highly reproducible method that contributes to an improvement of the techniques previously described and may contribute to future studies of the degeneration and regeneration of the facial nerve.
authors.affiliation: Department of Otolaryngology, University of São Paulo School of Medicine, São Paulo, Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: Salomone
authors.forename: Heloisa Juliana Zabeu Rossi
authors.initials: HJ
authors.lastname: Costa
authors.forename: José Ricardo Ferreira
authors.initials: JR
authors.lastname: Rodrigues
authors.forename: Samanta Marques
authors.initials: SM
authors.lastname: Reis e Silva
authors.forename: Patrícia Camacho
authors.initials: PC
authors.lastname: Ovando
authors.forename: Patricia Camacho
authors.initials: PC
authors.lastname: Orando
authors.forename: Ricardo Ferreira
authors.initials: RF
authors.lastname: Bento
mesh_terms: D000200:Action Potentials; D000818:Animals; D004558:Electric Stimulation; D004576:Electromyography; D005154:Facial Nerve / Q000502:physiology*; D008297:Male; D008334:Mandible / Q000294:innervation*; D023421:Models, Animal; D009416:Nerve Regeneration / Q000502:physiology*; D009431:Neural Conduction / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar