title: Biocatalytic resolution of benzyl glycidyl ether and its derivates by Talaromyces flavus: effect of phenyl ring substituents on enantioselectivity.
abstract: Talaromyces flavus containing a constitutive epoxide hydrolase (EH) resolved racemic benzyl glycidyl ether and nine derivatives into their (R)-enantiomers. After optimization of the fermentation conditions, the specific EH activity and biomass concentration were improved from 13.5 U/g DCW and 14.8 g DCW/l to 26.2 U/g DCW and 31.3 g DCW/l, respectively, with final values for e.e. ( s ) of 96 % and E of 13 with (R)-benzyl glycidyl ether. Substituents on the phenyl ring, however, gave low enantioselectivities.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310014, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Wei
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Chen
authors.forename: Honglei
authors.initials: H
authors.lastname: Shen
authors.forename: Shan
authors.initials: S
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D001593:Benzyl Compounds / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D018533:Biomass; D002384:Catalysis; D004851:Epoxide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D004852:Epoxy Compounds / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation; D013237:Stereoisomerism; D032901:Talaromyces / Q000201:enzymology / Q000378:metabolism*
chemical_list: D001593:Benzyl Compounds; D004852:Epoxy Compounds; C020344:glycidyl ethers; D004851:Epoxide Hydrolases