title: On-chip continuous monitoring of motile microorganisms on an ePetri platform.
abstract: Self-imaging Petri dish platforms with microscopy resolution, which we term 'ePetri', can significantly streamline cell cultures and/or other longitudinal biological studies. In this paper, we demonstrate high-resolution imaging and long-term culture of motile microorganisms in a specialized ePetri platform by taking advantage of the inherent motion. By applying a super-resolution algorithm to a set of low-resolution images of the microorganisms as they move across the sensing area of a complementary metal oxide semiconductor (CMOS) image sensor chip, we can render an improved-resolution image of the microorganisms. We perform a longitudinal study of Euglena gracilis cultured in an ePetri platform, and image-based analysis on the motion and morphology of the cells. As a miniaturized and automated culture monitoring platform, this ePetri technology can greatly improve studies and experiments with motile microorganisms.
authors.affiliation: Department of Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA. salee30@caltech.edu
authors.forename: Seung Ah
authors.initials: SA
authors.lastname: Lee
authors.forename: Guoan
authors.initials: G
authors.lastname: Zheng
authors.forename: Nandini
authors.initials: N
authors.lastname: Mukherjee
authors.forename: Changhuei
authors.initials: C
authors.lastname: Yang
mesh_terms: D000465:Algorithms; D005056:Euglena gracilis / Q000166:cytology*; D007091:Image Processing, Computer-Assisted; D008137:Longitudinal Studies; D008670:Metals / Q000737:chemistry; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D010087:Oxides / Q000737:chemistry; D012666:Semiconductors
chemical_list: D008670:Metals; D010087:Oxides