title: High-throughput immunoassay through in-channel microfluidic patterning.
abstract: We have developed an integrated microfluidic immunoassay chip for high-throughput sandwich immunoassay tests. The chip creates an array of reactive patterns through mechanical protection by actuating monolithically embedded button valves. We have demonstrated that this chip can achieve highly sensitive immunoassay tests within an hour, and requires only microliter samples.
authors.affiliation: College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Zheng
authors.forename: Jingwen
authors.initials: J
authors.lastname: Wang
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Pang
authors.forename: Jianbin
authors.initials: J
authors.lastname: Wang
authors.forename: Wenbin
authors.initials: W
authors.lastname: Li
authors.forename: Zigang
authors.initials: Z
authors.lastname: Ge
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Huang
mesh_terms: D002272:Carcinoembryonic Antigen / Q000097:blood; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods
chemical_list: D002272:Carcinoembryonic Antigen; D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; C013830:baysilon; D016650:Fluorescein-5-isothiocyanate