title: Proteomics-based identification of α-enolase as a potential prognostic marker in cholangiocarcinoma.
abstract: OBJECTIVE
  To investigate the association of expression status of α-enolase (ENO1) and clinicopathological outcomes of CCA patients.
  
  
  METHODS
  Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to compare differential expressed protein profiles of four human CCA cell lines and H69, a non-malignant biliary cell line, as a control. Immunohistochemical analysis was carried out in tissue-microarray of human CCA tissues (n=301).
  
  
  RESULTS
  We identified ENO1 in all CCA cell lines but not H69 by proteomics based. About 75% of patients with CCA showed over-expression of ENO1 in hyperplastic bile duct and the tumors compared with that in tumor-adjacent normal tissue counterparts. Moreover, over-expression of ENO1 is significantly associated with poor prognosis and tumor invasion of CCA patients.
  
  
  CONCLUSIONS
  ENO1 may serve as a prognostic marker to monitor the disease progression of these patients.
authors.affiliation: Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Ponlapat
authors.initials: P
authors.lastname: Yonglitthipagon
authors.forename: Chawalit
authors.initials: C
authors.lastname: Pairojkul
authors.forename: Vajarabhongsa
authors.initials: V
authors.lastname: Bhudhisawasdi
authors.forename: Jason
authors.initials: J
authors.lastname: Mulvenna
authors.forename: Alex
authors.initials: A
authors.lastname: Loukas
authors.forename: Banchob
authors.initials: B
authors.lastname: Sripa
mesh_terms: D000595:Amino Acid Sequence; D001650:Bile Duct Neoplasms / Q000378:metabolism* / Q000473:pathology; D001653:Bile Ducts, Intrahepatic; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D018281:Cholangiocarcinoma / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D010751:Phosphopyruvate Hydratase