title: Cell cycle dependent association of EBP50 with protein phosphatase 2A in endothelial cells.
abstract: Ezrin-radixin-moesin (ERM)-binding phosphoprotein 50 (EBP50) is a phosphorylatable PDZ domain-containing adaptor protein that is abundantly expressed in epithelium but was not yet studied in the endothelium. We report unusual nuclear localization of EBP50 in bovine pulmonary artery endothelial cells (BPAEC). Immunofluorescent staining and cellular fractionation demonstrated that EBP50 is present in the nuclear and perinuclear region in interphase cells. In the prophase of mitosis EBP50 redistributes to the cytoplasmic region in a phosphorylation dependent manner and during mitosis EBP50 co-localizes with protein phosphatase 2A (PP2A). Furthermore, in vitro wound healing of BPAEC expressing phospho-mimic mutant of EBP50 was accelerated indicating that EBP50 is involved in the regulation of the cell division. Cell cycle dependent specific interactions were detected between EBP50 and the subunits of PP2A (A, C, and Bα) with immunoprecipitation and pull-down experiments. The interaction of EBP50 with the Bα containing form of PP2A suggests that this holoenzyme of PP2A can be responsible for the dephosphorylation of EBP50 in cytokinesis. Moreover, the results underline the significance of EBP50 in cell division via reversible phosphorylation of the protein with cyclin dependent kinase and PP2A in normal cells.
authors.affiliation: Department of Medical Chemistry, University of Debrecen Medical and Health Science Center, Debrecen, Hungary.
authors.forename: Anita
authors.initials: A
authors.lastname: Boratkó
authors.forename: Pál
authors.initials: P
authors.lastname: Gergely
authors.forename: Csilla
authors.initials: C
authors.lastname: Csortos
mesh_terms: D000818:Animals; D002417:Cattle; D002453:Cell Cycle; D002460:Cell Line; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D054648:Protein Phosphatase 2 / Q000378:metabolism*; D011651:Pulmonary Artery / Q000166:cytology; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology
chemical_list: D010750:Phosphoproteins; D017923:Sodium-Hydrogen Exchangers; C093376:sodium-hydrogen exchanger regulatory factor; D054648:Protein Phosphatase 2