title: OIC-A006-loaded true bone ceramic heals rabbit critical-sized segmental radial defect.
abstract: It has been reported that OIC-A006, an osteogenically inducible compound, is able to promote osteogenesis in vitro and in vivo. In this study, we used a rabbit critical-sized segmental radial defect model (CSD) (15 mm) to analyse the osteogenic activity of OIC-A006 in non-cell-seeded tissue engineering bone substitutes. The scaffold carrier was bovine sintered bone "true bone ceramic (TBC)". OIC-A006 was delivered by PLGA (D,L-lactide-co-glycolide acid) microspheres. Drug-free PLGA microspheres and rhBMP-2-loaded PLGA microspheres were used as negative and positive control groups, respectively. Three kinds of composite were fabricated by coupling TBC, type-I collagen and the corresponding microspheres. The animals were randomly divided into 4 groups: (1) Group A: defect only, (2) group B:TBC/Collagen/drug-free-microspheres, (3) group C:TBC/Collagen/ OIC-A006-microspheres, (4) group D: TBC/Collagen/rhBMP-2-microspheres. The samples were analysed by histology, X-ray,microcomputed tomography (micro-CT), and biomechanical analyses. The results showed that OIC-A006 promoted bone regeneration to a remarkable extent. It is suggested that the application of OIC-A006 might be a valuable method in bone tissue engineering for healing large segmental defects of long bones. However, the biomechanical strength was a little inferior to that of BMPs.
authors.affiliation: Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and Orthopaedics, Department of Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Huarong
authors.initials: H
authors.lastname: Shao
authors.forename: Jin
authors.initials: J
authors.lastname: Shao
authors.forename: Hailin
authors.initials: H
authors.lastname: Bian
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Jin
authors.initials: J
authors.lastname: Qi
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Wang
authors.forename: Niandong
authors.initials: N
authors.lastname: Qian
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Lianfu
authors.initials: L
authors.lastname: Deng
mesh_terms: D000588:Amines / Q000627:therapeutic use*; D000818:Animals; D001696:Biomechanical Phenomena; D001846:Bone Development / Q000187:drug effects; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000473:pathology; D002516:Ceramics / Q000627:therapeutic use*; D005456:Fluorescent Dyes; D019344:Lactic Acid; D008297:Male; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D011884:Radius / Q000000981:diagnostic imaging / Q000473:pathology*; D015398:Signal Transduction; D023822:Tissue Engineering; D014057:Tomography, X-Ray Computed
chemical_list: D000588:Amines; D019485:Bone Morphogenetic Proteins; D005456:Fluorescent Dyes; C571980:OIC-A006; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid