title: [Construction of eukaryotic expression vectors for different domains of the extracellular region of RAGE and their expression in prostate cancer cells].
abstract: OBJECTIVE
  To construct eukaryotic expression vectors for different domains  (V and VC1) of the extracellular region of the receptor of advanced glycation end products (RAGE) and investigate the roles of these domains in prostate cancer.
  
  
  METHODS
  The coding sequence of V and VC1 domains was amplified from the plasmid pcDNA3-HA-RAGE by PCR and cloned into the pcDNA3-HA vector following routine procedures. After identification by PCR and sequencing, the vectors including V and VC1 domains were transfected into PC-3 cells. Western blotting and immunofluorescence were used to detect the expression and distribution of the expressed products in transfected PC-3 cells.
  
  
  RESULTS
  The expression vectors containing V and VC1 domains of RAGE were successfully constructed as confirmed by PCR and DNA sequence analysis. The V and VC1 domains of RAGE were highly expressed and showed a cytoplasmic distribution in transfected PC-3 cells.
  
  
  CONCLUSIONS
  The constructed eukaryotic expression vectors for V and VC1 domains of RAGE can be efficiently expressed in prostate cancer cells.
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. stevensmilelee@163.com
authors.forename: Jucong
authors.initials: J
authors.lastname: Li
authors.forename: Xianlu
authors.initials: X
authors.lastname: Song
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Li
authors.forename: Yingqia
authors.initials: Y
authors.lastname: Hong
authors.forename: Peng
authors.initials: P
authors.lastname: Deng
authors.forename: Chubiao
authors.initials: C
authors.lastname: Zhao
authors.forename: Haihua
authors.initials: H
authors.lastname: Luo
authors.forename: Shanchao
authors.initials: S
authors.lastname: Zhao
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D010957:Plasmids; D011471:Prostatic Neoplasms / Q000235:genetics*; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014162:Transfection
chemical_list: D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins