title: Identification and phylogenetic analysis of orf viruses isolated from outbreaks in goats of Assam, a northeastern state of India.
abstract: Two outbreaks of orf virus (ORFV) (a parapoxvirus) infection in goats, which occurred in Golaghat and Kamrup districts of Assam, a northeastern part of India, were investigated. The disease was diagnosed by standard virological and molecular techniques. The entire protein-coding region of B2L gene of two isolates were cloned and sequenced. Phylogenetic analysis based on B2L amino acid sequences showed that the ORFVs identified in these outbreaks were closely related to each other and both were closer to ORFV-Shahjahanpur 82/04 isolate from north India. The present study revealed that the precise characterization of the genomic region (B2L gene) might provide evidence for the genetic variation and movement of circulating ORFV strains in India.
authors.affiliation: Pox Virus Laboratory, Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital District, Kumaon, 263 138 Uttarakhand, India. drdpbora@sify.com
authors.forename: Durlav Prasad
authors.initials: DP
authors.lastname: Bora
authors.forename: Nagendra Nath
authors.initials: NN
authors.lastname: Barman
authors.forename: Sailendra Kumar
authors.initials: SK
authors.lastname: Das
authors.forename: Veerakyathappa
authors.initials: V
authors.lastname: Bhanuprakash
authors.forename: Revanaiah
authors.initials: R
authors.lastname: Yogisharadhya
authors.forename: Gnanavel
authors.initials: G
authors.lastname: Venkatesan
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.forename: Gitika
authors.initials: G
authors.lastname: Rajbongshi
authors.forename: Elina
authors.initials: E
authors.lastname: Khatoon
authors.forename: Apurba
authors.initials: A
authors.lastname: Chakraborty
authors.forename: Kamal Malla
authors.initials: KM
authors.lastname: Bujarbaruah
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004196:Disease Outbreaks; D004474:Ecthyma, Contagious / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D015511:Goat Diseases / Q000453:epidemiology* / Q000821:virology; D006041:Goats; D007194:India / Q000453:epidemiology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009923:Orf virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D017422:Sequence Analysis, DNA