title: HSF is the most important transcriptional factor for porcine MC4R promoter.
abstract: The melanocortin-4 receptor (MC4R) is involved in feed intake regulation. It is significantly associated with growth and fatness traits in most breeds and crosses. To understand the essential transcriptional regions of the porcine MC4R promoter, eleven primer pairs were designed to amplify different segment lengths of the MC4R promoter. The PCR products were then ligated along with the Firefly luciferase reporter gene into the PGL3-basic vector. The results showed that a 90 bp fragment could contain the essential regions for control of transcription. Further research found that a short sequence "AGAAAGAAG" (the recognition site of transcription factor HSF) was the most important sequence for supporting activity of the pig MC4R promoter.
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, China. zdj8109@163.com
authors.forename: D J
authors.initials: DJ
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: X H
authors.initials: XH
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Bie
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D017930:Genes, Reporter; D049409:Luciferases, Firefly / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics* / Q000378:metabolism*; D013552:Swine; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D044105:Receptor, Melanocortin, Type 4; D014157:Transcription Factors; D049409:Luciferases, Firefly