title: Metagenomic analysis of novel lignocellulose-degrading enzymes from higher termite guts inhabiting microbes.
abstract: A metagenomic fosmid library was constructed from genomic DNA isolated from the microbial community residing in hindguts of a wood-feeding higher termite (Microcerotermes sp.) collected in Thailand. The library was screened for clones expressing lignocellulolytic activities. Fourteen independent active clones (2 cellulases and 12 xylanases) were obtained by functional screening at pH 10.0. Analysis of shotgun-cloning and pyrosequencing data revealed six ORFs, which shared less than 59% identity and 73% similarity of their amino acid sequences with known cellulases and xylanases. Conserved domain analysis of these ORFs revealed a cellulase belonging to the glycoside hydrolase family 5, whereas the other five xylanases showed significant identity to diverse families including families 8, 10, and 11. Interestingly, one fosmid clone was isolated carrying three contiguous xylanase genes that may comprise a xylanosome operon. The enzymes with the highest activities at alkaline pH from the initial activity screening were characterized biochemically. These enzymes showed a broad range of enzyme activities from pH 5.0 to 10.0, with pH optimal of 8.0 retaining more than 70% of their respective activities at pH 9.0. The optimal temperatures of these enzymes ranged from 50 degrees C to 55 degrees C. This study provides evidence for the diversity and function of lignocellulose-degrading enzymes in the termite gut microbial community, which could be of potential use for industrial processes such as pulp biobleaching and denim biostoning.
authors.affiliation: Enzyme Technology Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology-BIOTEC, Thailand Science Park, Pathumthani 12120, Thailand. thidarat.nim@biotec.or.th
authors.forename: Thidarat
authors.initials: T
authors.lastname: Nimchua
authors.forename: Taksawan
authors.initials: T
authors.lastname: Thongaram
authors.forename: Tanaporn
authors.initials: T
authors.lastname: Uengwetwanit
authors.forename: Somchai
authors.initials: S
authors.lastname: Pongpattanakitshote
authors.forename: Lily
authors.initials: L
authors.lastname: Eurwilaichitr
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D044602:Cellulases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004795:Enzyme Stability; D041981:Gastrointestinal Tract / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D020049:Isoptera / Q000378:metabolism / Q000382:microbiology*; D008031:Lignin / Q000378:metabolism*; D056186:Metagenomics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D014995:Xylosidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C036909:lignocellulose; D008031:Lignin; D044602:Cellulases; D014995:Xylosidases