title: A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein.
abstract: In this study, we report a facile method for the preparation of core-shell magnetic molecularly imprinted polymers (MIPs) for protein recognition. Uniform carboxyl group functionalized Fe(3)O(4) nanoparticles (NPs) were synthesized using a solvothermal method. Magnetic MIPs were synthesized by self-polymerization of dopamine in the presence of template protein on the surface of the Fe(3)O(4) NPs. A thin layer of polydopamine can be coated on Fe(3)O(4) NPs via dopamine self-polymerization and the imprinted polydopamine shells can be controlled by the mass ratio of Fe(3)O(4) NPs and dopamine. More importantly, there is a critical value of polydopamine shell thickness for the maximum rebinding capacity. The as-prepared lysozyme-imprinted Fe(3)O(4)@polydopamine NPs show high binding capacity and acceptable specific recognition behavior towards template proteins. This method provides the possibility for the separation and enrichment of abundant proteins in proteomic analysis.
authors.affiliation: State Key Laboratory of Medical Chemical Biology, Nankai University, Tianjin 300071, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Xihao
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiwen
authors.initials: X
authors.lastname: He
authors.forename: Langxing
authors.initials: L
authors.lastname: Chen
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D052203:Ferrosoferric Oxide / Q000737:chemistry; D007211:Indoles / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D054802:Molecular Imprinting; D009113:Muramidase / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D007211:Indoles; D058185:Magnetite Nanoparticles; D011108:Polymers; C568283:polydopamine; D009113:Muramidase; D052203:Ferrosoferric Oxide