title: Inhibition of HSV-1 by chemoattracted neutrophils: supernatants of corneal epithelial cells (HCE) and macrophages (THP-1) treated with virus components chemoattract neutrophils (PMN), and supernatants of PMN treated with these conditioned media inhibit viral growth.
abstract: The role of PMNs (neutrophils) in corneal herpes was studied using an in vitro system. Human corneal cells (HCE) and macrophages (THP-1) infected with HSV-1 or treated with virus components (DNA or virus immune complexes) released chemokines, which attracted PMNs. Highly reactive oxygen species were detected in PMNs. PMNs inhibited HSV when overlaid onto infected HCE cells (50:1). PMNs incubated with the supernatants of HCE cells treated with virus components released H(2)O(2) and myeloperoxidase. These inhibited virus growth. PMNs released NO and MIG, which may differentiate CD4 T cells to Th1. PMNs participate in innate immune responses, limit virus growth, and initiate immunopathology.
authors.affiliation: Immunology and Virology Section, Laboratory of Immunology, National Eye Institute, NIH, Bethesda, MD 20892, USA. kozaburo@yc4.so-net.ne.jp
authors.forename: Kozaburo
authors.initials: K
authors.lastname: Hayashi
authors.forename: Laura C
authors.initials: LC
authors.lastname: Hooper
authors.forename: Toshiomi
authors.initials: T
authors.lastname: Okuno
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Takada
authors.forename: John J
authors.initials: JJ
authors.lastname: Hooks
mesh_terms: D002478:Cells, Cultured; D002633:Chemotaxis; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D019573:Epithelium, Corneal / Q000166:cytology*; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D009195:Peroxidase / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
chemical_list: D017077:Culture Media, Conditioned; D006861:Hydrogen Peroxide; D009195:Peroxidase