title: A laccase with HIV-1 reverse transcriptase inhibitory activity from the broth of mycelial culture of the mushroom Lentinus tigrinus.
abstract: A 59 kDa laccase with inhibitory activity against HIV-1 reverse transcriptase (IC(50) = 2.4 μM) was isolated from the broth of mycelial culture of the mushroom Lentinus tigrinus. The isolation procedure involved ion exchange chromatography on DEAE-cellulose and CM-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The laccase was adsorbed on both types of ion exchangers. About 95-fold purification was achieved with a 25.9% yield of the enzyme. The procedure resulted in a specific enzyme activity of 76.6 U/mg. Its N-terminal amino acid sequence was GIPDLHDLTV, which showed little similarity to other mushroom laccase and other Lentinus tigrinus strain laccase. Its characteristics were different from previously reported laccase of other Lentinus tigrinus strain. Maximal laccase activity was observed at a pH of 4 and at a temperature of 60°C, respectively. This study yielded the information about the potentially exploitable activities of Lentinus tigrinus laccase.
authors.affiliation: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China.
authors.forename: LiJing
authors.initials: L
authors.lastname: Xu
authors.forename: HeXiang
authors.initials: H
authors.lastname: Wang
authors.forename: TziBun
authors.initials: T
authors.lastname: Ng
mesh_terms: D000595:Amino Acid Sequence; D002852:Chromatography, Ion Exchange; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D042845:Laccase / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D020074:Lentinula / Q000737:chemistry / Q000201:enzymology*; D008969:Molecular Sequence Data; D025282:Mycelium / Q000737:chemistry / Q000201:enzymology; D018894:Reverse Transcriptase Inhibitors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D013696:Temperature
chemical_list: D005656:Fungal Proteins; D018894:Reverse Transcriptase Inhibitors; D042845:Laccase; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase