title: Impact of 4HPR on the expression of E-Cad in human bladder transitional epithelial cancer cells T24.
abstract: Previous researches showed that the expression level of E-Cad in most infiltrating cancer cells was reduced or negative. This study explored whether 4HPR restrained the infiltration of bladder cancer cells through regulating the expression of E-Cad. The infiltrating bladder cancer cells T24 were cultured, and then treated by a proper dosage of drug. Their viability was a determined by MTT method. Western blotting and RT-PCR were adopted to detect the changes of E-Cad gene expression at both protein and mRNA levels. Moreover, immunofluorescent staining and confocal fluorescence microscopy were employed for the observation of the expression of E-Cad. The result showed that, at both mRNA and protein levels, the expression level of E-Cad in T24 cells treated by 4HPR was significantly higher than that of control group, while the β-Cat expression was also relocated from the cell nucleus to cytoplasm. Our findings suggested that the regulatory function of 4HPR on infiltration of bladder cancer cells T24 is at least partly achieved by regulating the expression of E-Cad.
authors.affiliation: School of Basic Medical Science, Wuhan University, Wuhan, 430071, China.|Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors.forename: Eyou
authors.initials: E
authors.lastname: Wang
authors.affiliation: School of Basic Medical Science, Wuhan University, Wuhan, 430071, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Basic Medical Science, Wuhan University, Wuhan, 430071, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Yang
authors.affiliation: School of Basic Medical Science, Wuhan University, Wuhan, 430071, China. zhongshan@whu.edu.cn.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhong
authors.affiliation: Zhongnan Hospital of Wuhan University, Wuhan, 430071, China. liutongzu@163.com.
authors.forename: Tongzu
authors.initials: T
authors.lastname: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects; D015820:Cadherins / Q000378:metabolism*; D002295:Carcinoma, Transitional Cell / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009361:Neoplasm Invasiveness; D014212:Tretinoin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D015820:Cadherins; D014212:Tretinoin; C058346:N-(4-methoxyphenyl)retinamide