title: [Influence of S-nitrosoglutathione on agglutination and nitric oxide concentration in frozen platelets].
abstract: The aim of this study was to investigate the influence of S-nitrosoglutathione (GSNO) on agglutination and nitric oxide (NO) concentration in frozen platelets. The agglutination of platelets was detected by using platelet agglutination apparatus, the level of NO in platelets was detected by the nitrate enzyme reduction method. The results showed that the rates of agglutination in freeze platelets and frozen platelets treated with GSNO were (35.47 ± 2.93) and (24.43 ± 3.07), which were significantly lower than that in fresh liquid platelets (63.44 ± 2.96). The level of NO concentration in frozen platelets was (22.16 ± 6.38), which was significantly lower than that in fresh liquid platelets (31.59 ± 16.88). The level of NO concentration in frozen platelets treated with GSNO was (45.64 ± 6.31), which was significantly higher than that in fresh liquid platelets (P < 0.01). It is concluded that GSNO increases the concentration of NO in frozen platelets, inhibits platelet activation and maintains platelet function, thus GSNO can be used as a frozen protective agent.
authors.affiliation: Department of Blood Transfusion, Chinese PLA General Hospital, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Jing-Han
authors.initials: JH
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Wu
authors.initials: W
authors.lastname: Zhou
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Wang
mesh_terms: D001792:Blood Platelets / Q000187:drug effects; D005615:Freezing; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism*; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects*; D010976:Platelet Count; D026422:S-Nitrosoglutathione / Q000494:pharmacology*
chemical_list: D009569:Nitric Oxide; D026422:S-Nitrosoglutathione