title: Human placental decidua basalis-derived mesenchymal stem cells differentiate into dopamine neuron-like cells with no response to long-term culture in vitro.
abstract: Human placental decidua basalis-derived mesenchymal stem cells (DBMSCs) have been identified as valuable sources for cell transplantation. In this study, we found that DBMSCs could be induced to form neural stem cells in the form of neurospheres. These neurospheres were further differentiated into dopamine neuron-like cells with a cocktail of cytokines. The differentiated DBMSCs were verified through the presence of a neuron-like morphology, the expression of specific dopamine neuron makers, and the production of dopamine. In addition, this differentiation capacity of DBMSCs was not affected by long-term culture, and the cells maintained a normal karyotype in vitro. The dopamine neuronal differentiation and the relative safety transplantation potential of DBMSCs may facilitate stem cell therapeutic approaches to Parkinson's disease.
authors.affiliation: Department of Neurosurgery, Institute of Neurosurgery, Key Laboratory on Brain Function Repair and Regeneration of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Guo-hui
authors.initials: GH
authors.lastname: Lu
authors.forename: Wang-shi
authors.initials: WS
authors.lastname: Yong
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Xu
authors.forename: Yi-quan
authors.initials: YQ
authors.lastname: Ke
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Jiang
authors.forename: Shi-zhong
authors.initials: SZ
authors.lastname: Zhang
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003656:Decidua / Q000166:cytology*; D004298:Dopamine / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000502:physiology*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007621:Karyotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011247:Pregnancy
chemical_list: D015703:Antigens, CD; D009419:Nerve Tissue Proteins; D004298:Dopamine