title: Antibody-directed lentiviral gene transduction for live-cell monitoring and selection of human iPS and hES cells.
abstract: The identification of stem cells within a mixed population of cells is a major hurdle for stem cell biology--in particular, in the identification of induced pluripotent stem (iPS) cells during the reprogramming process. Based on the selective expression of stem cell surface markers, a method to specifically infect stem cells through antibody-conjugated lentiviral particles has been developed that can deliver both visual markers for live-cell imaging as well as selectable markers to enrich for iPS cells. Antibodies recognizing SSEA4 and CD24 mediated the selective infection of the iPS cells over the parental human fibroblasts, allowing for rapid expansion of these cells by puromycin selection. Adaptation of the vector allows for the selective marking of human embryonic stem (hES) cells for their removal from a population of differentiated cells. This method has the benefit that it not only identifies stem cells, but that specific genes, including positive and negative selection markers, regulatory genes or miRNA can be delivered to the targeted stem cells. The ability to specifically target gene delivery to human pluripotent stem cells has broad applications in tissue engineering and stem cell therapies.
authors.affiliation: Department of Pharmacology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey, United States of America.
authors.forename: Dai-tze
authors.initials: DT
authors.lastname: Wu
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Seita
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.forename: Chi-Wei
authors.initials: CW
authors.lastname: Lu
authors.forename: Monica J
authors.initials: MJ
authors.lastname: Roth
mesh_terms: D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D005434:Flow Cytometry; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D006801:Humans; D018796:Immunoconjugates / Q000235:genetics* / Q000378:metabolism; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D016086:Lentivirus / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D014161:Transduction, Genetic; D019076:Transgenes; D014771:Virion / Q000235:genetics; D053586:Virus Internalization
chemical_list: D000954:Antigens, Surface; D018796:Immunoconjugates; D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins