title: Live cell imaging of a fluorescent gentamicin conjugate.
abstract: Understanding cellular mechanisms of ototoxic and nephrotoxic drug uptake, intracellular distribution, and molecular trafficking across cellular barrier systems aids the study of potential uptake blockers that preserve sensory and renal function during critical life-saving therapy. Herein we report the design, synthesis characterization and evaluation of a fluorescent conjugate of the aminoglycoside antibiotic gentamicin. Live cell imaging results show the potential utility of this new material. Related gentamicin conjugates studied to date quench in live kindney cells, and have been largely restricted to use in fixed (delipidated) cells.
authors.affiliation: Department of Chemistry, Portland State University, Portland, OR 97201, USA.
authors.forename: Jorge O
authors.initials: JO
authors.lastname: Escobedo
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Chu
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Peter S
authors.initials: PS
authors.lastname: Steyger
authors.forename: Robert M
authors.initials: RM
authors.lastname: Strongin
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D005456:Fluorescent Dyes / Q000737:chemistry*; D005839:Gentamicins / Q000737:chemistry / Q000493:pharmacokinetics*; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000493:pharmacokinetics*; D014966:Xanthenes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D005839:Gentamicins; D011500:Protein Synthesis Inhibitors; D014966:Xanthenes; C034657:Texas red