title: Nanophotonic lab-on-a-chip platforms including novel bimodal interferometers, microfluidics and grating couplers.
abstract: One of the main limitations for achieving truly lab-on-a-chip (LOC) devices for point-of-care diagnosis is the incorporation of the "on-chip" detection. Indeed, most of the state-of-the-art LOC devices usually require complex read-out instrumentation, losing the main advantages of portability and simplicity. In this context, we present our last advances towards the achievement of a portable and label-free LOC platform with highly sensitive "on-chip" detection by using nanophotonic biosensors. Bimodal waveguide interferometers fabricated by standard silicon processes have been integrated with sub-micronic grating couplers for efficient light in-coupling, showing a phase resolution of 6.6 × 10(-4)× 2π rad and a limit of detection of 3.3 × 10(-7) refractive index unit (RIU) in bulk. A 3D network of SU-8 polymer microfluidics monolithically assembled at the wafer-level was included, ensuring perfect sealing and compact packaging. To overcome some of the drawbacks inherent to interferometric read-outs, a novel all-optical wavelength modulation system has been implemented, providing a linear response and a direct read-out of the phase variation. Sensitivity, specificity and reproducibility of the wavelength modulated BiMW sensor has been demonstrated through the label-free immunodetection of the human hormone hTSH at picomolar level using a reliable biofunctionalization process.
authors.affiliation: Nanobiosensors and Bioanalytical Applications Group (CIN2) CSIC and CIBER-BBN, Campus UAB, Barcelona, Spain.
authors.forename: Daphné
authors.initials: D
authors.lastname: Duval
authors.forename: Ana Belén
authors.initials: AB
authors.lastname: González-Guerrero
authors.forename: Stefania
authors.initials: S
authors.lastname: Dante
authors.forename: Johann
authors.initials: J
authors.lastname: Osmond
authors.forename: Rosa
authors.initials: R
authors.lastname: Monge
authors.forename: Luis J
authors.initials: LJ
authors.lastname: Fernández
authors.forename: Kirill E
authors.initials: KE
authors.lastname: Zinoviev
authors.forename: Carlos
authors.initials: C
authors.lastname: Domínguez
authors.forename: Laura M
authors.initials: LM
authors.lastname: Lechuga
mesh_terms: D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques; D006801:Humans; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics / Q000295:instrumentation* / Q000379:methods; D012031:Refractometry; D013972:Thyrotropin / Q000032:analysis / Q000276:immunology
chemical_list: D000906:Antibodies; D013972:Thyrotropin