title: A successful diagnostic case of Pneumocystis pneumonia by the loop-mediated isothermal amplification method in a patient with dermatomyositis.
abstract: Pneumocystis pneumonia (PCP) can occur in patients with many causes of the immunocompromised state other than human immunodeficiency virus (HIV). It is quite difficult to diagnose PCP without HIV because there is no method for detecting Pneumocystis jirovecii. Thus, non-HIV PCP continues to have high mortality. Recently, loop-mediated isothermal amplification (LAMP) is becoming an established nucleic acid amplification method offering rapid, accurate, and cost-effective diagnosis of infectious diseases. We report a non-HIV PCP case successfully diagnosed by the LAMP method. It was previously reported that PCR in BALF specimens had been the most sensitive method in the diagnosis of PCP without HIV. The LAMP method would be more sensitive than conventional PCR and an effective tool in the early diagnosis of PCP.
authors.affiliation: Department of Pulmonology, Kameda Medical Center, 929 Higashi-cho, Kamogawa, Chiba 296-8602, Japan. nobuhiro0204@hotmail.com
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Asai
authors.forename: Masahiro
authors.initials: M
authors.lastname: Aoshima
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ohkuni
authors.forename: Haruki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Ryo
authors.initials: R
authors.lastname: Matsunuma
authors.forename: Kei
authors.initials: K
authors.lastname: Nakashima
authors.forename: Naoko
authors.initials: N
authors.lastname: Katsurada
authors.forename: Hiroto
authors.initials: H
authors.lastname: Nakano
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Matsui
authors.forename: Norihiro
authors.initials: N
authors.lastname: Kaneko
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D003882:Dermatomyositis / Q000382:microbiology*; D006801:Humans; D008168:Lung / Q000473:pathology; D008297:Male; D008875:Middle Aged; D058889:Molecular Typing / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D045363:Pneumocystis carinii / Q000302:isolation & purification; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D013902:Radiography, Thoracic