title: The expression of prophenoloxidase mRNA in red swamp crayfish, Procambarus clarkii, when it was challenged.
abstract: The expression of the prophenoloxidase (proPO) gene was investigated in nine tissues of red swamp crayfish Procambarus clarkii, by real-time PCR after challenges by CpG oligodeoxynucleotide (ODN), Aeromonas hydrophila and white spot syndrome virus (WSSV). The results can be summarized as follows: (i) the expression level of the proPO gene in haemocytes was highest among nine studied tissues before the challenge; (ii) the expression of proPO increased in all studied tissues after stimulation by CpG ODN and WSSV, and also increased in all tissues, except the ovary, after the A. hydrophila challenge; (iii) the whole expression profiles were different, suggesting that different immune mechanisms may exist for crayfish that are resistant to WSSV and A. hydrophila, although the expression in haemocytes was similar before and after the WSSV and A. hydrophila challenges.
authors.affiliation: College of Fisheries, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Deng
authors.forename: Keli
authors.initials: K
authors.lastname: Yang
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
mesh_terms: D016980:Aeromonas hydrophila / Q000472:pathogenicity; D000818:Animals; D003400:Astacoidea / Q000201:enzymology* / Q000235:genetics*; D004156:Catechol Oxidase / Q000235:genetics* / Q000378:metabolism*; D004792:Enzyme Precursors / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D006434:Hemocytes / Q000378:metabolism / Q000382:microbiology / Q000821:virology; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism; D010053:Ovary / Q000378:metabolism / Q000382:microbiology / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015854:Up-Regulation; D046848:White spot syndrome virus 1 / Q000472:pathogenicity
chemical_list: D004792:Enzyme Precursors; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C038451:pro-phenoloxidase; D004156:Catechol Oxidase