title: Surface modified liposomes by mannosylated conjugates anchored via the adamantyl moiety in the lipid bilayer.
abstract: The aim of the present study was to encapsulate mannosylated 1-aminoadamantane and mannosylated adamantyltripeptides, namely [(2R)-N-(adamant-1-yl)-3-(α,β-d-mannopyranosyloxy)-2-methylpropanamide and (2R)-N-[3-(α-d-mannopyranosyloxy)-2-methylpropanoyl]-d,l-(adamant-2-yl)glycyl-l-alanyl-d-isoglutamine] in liposomes. The characterization of liposomes, size and surface morphology was performed using dynamic light scattering (DLS) and atomic force microscopy (AFM). The results have revealed that the encapsulation of examined compounds changes the size and surface of liposomes. After the concanavalin A (ConA) was added to the liposome preparation, increase in liposome size and their aggregation has been observed. The enlargement of liposomes was ascribed to the specific binding of the ConA to the mannose present on the surface of the prepared liposomes. Thus, it has been shown that the adamantyl moiety from mannosylated 1-aminoadamantane and mannosylated adamantyltripeptides can be used as an anchor in the lipid bilayer for carbohydrate moiety exposed on the liposome surface.
authors.affiliation: Institute of Immunology, Inc., Zagreb, Croatia.
authors.forename: Adela
authors.initials: A
authors.lastname: Stimac
authors.forename: Suzana
authors.initials: S
authors.lastname: Segota
authors.forename: Maja
authors.initials: M
authors.lastname: Dutour Sikirić
authors.forename: Rosana
authors.initials: R
authors.lastname: Ribić
authors.forename: Leo
authors.initials: L
authors.lastname: Frkanec
authors.forename: Vesna
authors.initials: V
authors.lastname: Svetličić
authors.forename: Srđanka
authors.initials: S
authors.lastname: Tomić
authors.forename: Branka
authors.initials: B
authors.lastname: Vranešić
authors.forename: Ruža
authors.initials: R
authors.lastname: Frkanec
mesh_terms: D001703:Biophysics / Q000379:methods; D002845:Chromatography / Q000379:methods; D003208:Concanavalin A / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D008956:Models, Chemical; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry*; D012542:Scattering, Radiation; D055672:Static Electricity; D013499:Surface Properties; D014461:Ultracentrifugation
chemical_list: D037102:Lectins; D008051:Lipid Bilayers; D008081:Liposomes; D010455:Peptides; D003208:Concanavalin A; D008358:Mannose