title: Identification of food-derived elastin peptide, prolyl-glycine (Pro-Gly), in human blood after ingestion of elastin hydrolysate.
abstract: Elastin hydrolysate has apparent beneficial effects, and the food-derived peptide prolyl-glycine (Pro-Gly) is present in human blood after oral ingestion. Following ingestion of elastin hydrolysate (10 g/60 kg body weight) by healthy human volunteers, peripheral blood was used to prepare plasma samples from which peptides were extracted by solid phase extraction and fractionated by size-exclusion chromatography (SEC). Peptides in the SEC fractions were derivatized with phenyl isothiocyanate (PITC) and resolved by reversed phase (RP)-HPLC. Pro-Gly was the major food-derived elastin peptide, reaching a maximum (18 μM) at 30 min after ingestion, and decreasing to approximately 20% at 4 h after ingestion. Finally, in cell culture, levels of Pro-Gly in the medium above 0.1 μg/mL significantly enhanced elastin synthesis of normal human dermal fibroblasts (NHDF) without affecting the rate of cell proliferation.
authors.affiliation: Department of Nutrition, Faculty of Domestic Science, Tokyo Kasei University, 1-18-1 Kaga, Itabashi-ku, Tokyo, 173-8602, Japan.
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Shigemura
authors.forename: Misako
authors.initials: M
authors.lastname: Nakaba
authors.forename: Eri
authors.initials: E
authors.lastname: Shiratsuchi
authors.forename: Masashi
authors.initials: M
authors.lastname: Suyama
authors.forename: Michio
authors.initials: M
authors.lastname: Yamada
authors.forename: Tamami
authors.initials: T
authors.lastname: Kiyono
authors.forename: Kazuna
authors.initials: K
authors.lastname: Fukamizu
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Park
authors.forename: Yasusi
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Kenji
authors.initials: K
authors.lastname: Sato
mesh_terms: D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D004151:Dipeptides / Q000097:blood*; D004549:Elastin / Q000008:administration & dosage* / Q000097:blood*; D005347:Fibroblasts / Q000378:metabolism; D005502:Food; D006801:Humans; D051379:Mice; D008875:Middle Aged; D011492:Protein Hydrolysates / Q000008:administration & dosage*
chemical_list: D004151:Dipeptides; D011492:Protein Hydrolysates; C057749:prolylglycine; D004549:Elastin