title: Microsatellite primers in the endangered quillwort Isoetes hypsophila (Isoetaceae) and cross-amplification in I. sinensis.
abstract: OBJECTIVE
  The first microsatellite primers were developed for Isoetes hypsophila, an endangered quillwort species endemic to the Qinghai-Tibetan Plateau in China, to further describe its genetic variability and population structure. We also examined their cross-amplification in a congeneric species, I. sinensis.
  
  
  RESULTS
  Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila. The primers amplified di- and hexanucleotide repeats with three to 11 alleles per locus. Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus.
  
  
  CONCLUSIONS
  The microsatellite loci primers will be useful for studies of genetic diversity and gene flow in natural populations of Isoetes species.
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, People's Republic of China. lizz@wbgcas.cn
authors.forename: Zuozhou
authors.initials: Z
authors.lastname: Li
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Han
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D002681:China; D017931:DNA Primers / Q000378:metabolism*; D017753:Ecosystem; D056727:Endangered Species; D029624:Ferns / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers