title: Low-intensity pulsed ultrasound enhances BMP-7-induced osteogenic differentiation of human fracture hematoma-derived progenitor cells in vitro.
abstract: OBJECTIVE
  To investigate the effect of the combined application of bone morphogenetic protein-7 (BMP-7) and low-intensity pulsed ultrasound (LIPUS) on human fracture hematoma-derived progenitor cells (HCs).
  
  
  METHODS
  HCs were isolated from 6 patients. The cells were then divided into 4 groups and cultured: (1) control group, HCs cultured in growth medium without LIPUS; (2) LIPUS group, HCs cultured in growth medium with LIPUS; (3) BMP-7 group, HCs cultured in osteogenic medium containing BMP-7 without LIPUS; and (4) BMP-7 + LIPUS group, HCs cultured in osteogenic medium with LIPUS. Osteogenic differentiation potential and proliferation of HCs were compared among 4 groups.
  
  
  RESULTS
  Alkaline phosphatase activity, the expression of osteogenic genes, and the mineralization of HCs in BMP-7 + LIPUS group were shown to be significantly increased compared with the other groups. However, LIPUS did not affect the proliferation of HCs in the presence or absence of BMP-7.
  
  
  CONCLUSIONS
  These findings demonstrated for the first time the significant effect of LIPUS on the osteogenic differentiation of HCs in the presence of BMP-7. This study may provide significant evidence for the clinical combined application of BMP-7 and LIPUS for the treatment of acute bone fractures.
authors.affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Sang Yang
authors.initials: SY
authors.lastname: Lee
authors.forename: Akihiro
authors.initials: A
authors.lastname: Koh
authors.forename: Takahiro
authors.initials: T
authors.lastname: Niikura
authors.forename: Keisuke
authors.initials: K
authors.lastname: Oe
authors.forename: Takaaki
authors.initials: T
authors.lastname: Koga
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Dogaki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kurosaka
mesh_terms: D000328:Adult; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D050723:Fractures, Bone / Q000473:pathology*; D006406:Hematoma / Q000473:pathology*; D006801:Humans; D008875:Middle Aged; D010012:Osteogenesis / Q000187:drug effects*; D013234:Stem Cells; D014463:Ultrasonography; D055815:Young Adult
chemical_list: D055419:Bone Morphogenetic Protein 7