title: Quantitative proteomic analysis of HIV-1 infected CD4+ T cells reveals an early host response in important biological pathways: protein synthesis, cell proliferation, and T-cell activation.
abstract: Human immunodeficiency virus (HIV-1) depends upon host-encoded proteins to facilitate its replication while at the same time inhibiting critical components of innate and/or intrinsic immune response pathways. To characterize the host cell response on protein levels in CD4+ lymphoblastoid SUP-T1 cells after infection with HIV-1 strain LAI, we used mass spectrometry (MS)-based global quantitation with iTRAQ (isobaric tag for relative and absolute quantification). We found 266, 60 and 22 proteins differentially expressed (DE) (P-value ≤ 0.05) at 4, 8, and 20 hours post-infection (hpi), respectively, compared to time-matched mock-infected samples. The majority of changes in protein abundance occurred at an early stage of infection well before the de novo production of viral proteins. Functional analyses of these DE proteins showed enrichment in several biological pathways including protein synthesis, cell proliferation, and T-cell activation. Importantly, these early changes before the time of robust viral production have not been described before.
authors.affiliation: Department of Microbiology, University of Washington, Seattle, WA 98195-8070, USA.
authors.forename: Arti T
authors.initials: AT
authors.lastname: Navare
authors.forename: Pavel
authors.initials: P
authors.lastname: Sova
authors.forename: David E
authors.initials: DE
authors.lastname: Purdy
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Weiss
authors.forename: Alejandro
authors.initials: A
authors.lastname: Wolf-Yadlin
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Korth
authors.forename: Stewart T
authors.initials: ST
authors.lastname: Chang
authors.forename: Sean C
authors.initials: SC
authors.lastname: Proll
authors.forename: Tahmina A
authors.initials: TA
authors.lastname: Jahan
authors.forename: Alexei L
authors.initials: AL
authors.lastname: Krasnoselsky
authors.forename: Robert E
authors.initials: RE
authors.lastname: Palermo
authors.forename: Michael G
authors.initials: MG
authors.lastname: Katze
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry* / Q000166:cytology / Q000276:immunology / Q000821:virology; D002460:Cell Line; D049109:Cell Proliferation; D053263:Gene Regulatory Networks; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology / Q000503:physiopathology / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D014176:Protein Biosynthesis; D040901:Proteomics; D013601:T-Lymphocytes / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism / Q000821:virology