title: [Effects of tumor necrosis factor-α on nutritional status and proteolysis of respiratory muscles in rats with chronic obstructive pulmonary disease].
abstract: OBJECTIVE
  To investigate the effect of tumor necrosis factor-α (TNF-α) on nutritional status and proteolysis of respiratory muscle in a rat model of chronic obstructive pulmonary disease (COPD).
  
  
  METHODS
  Ninety healthy male adult Wistar rats were randomly divided into model group (A) and normal control group (B). COPD malnutrition rat models were established by cigarettes smoke and nutrient limitation and divided into normal nutrition COPD group (A(1)), malnutrition COPD group (A(2)), and malnutrition COPD intervention group (A(3)). In group A(3), the rats received intravenous injection of TNF-α mAb (0.1 mg/kg). TNF-α levels in the serum and respiratory muscle homogenates were measured using enzyme-linked immunosorbent assay (ELISA), and plasma levels of glucose, albumin, and triglyceride were measured with an automatic biochemistry analyzer. High-performance liquid chromatography was used to measure the contents of 3-methylhistidine and tyrosine in the respiratory muscle homogenates.
  
  
  RESULTS
  The serum TNF-α level and plasma levels of glucose and triglyceride were significantly higher but the plasma albumin level was significantly lower in group A(2) than in groups B, A(1), and A(3) (P<0.01). The contents of 3-MH and Tyr in the respiratory muscle homogenates were significantly higher in group A(2) than in the other 3 groups (P<0.01, P<0.01). TNF-α in the respiratory muscle showed a strong positive correlation to 3-MH and Tyr.
  
  
  CONCLUSIONS
  TNF-α is one of the causes of increased proteolysis of the respiratory muscle.
authors.affiliation: Department of Respiratory Diseases, Third Xiangya Hospital of Central South University, Changsha 410013, China. liujianming101@sina.com
authors.forename: Jianming
authors.initials: J
authors.lastname: Liu
authors.forename: Qiande
authors.initials: Q
authors.lastname: Liao
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Tang
authors.forename: Shenghua
authors.initials: S
authors.lastname: Sun
authors.forename: Beizhan
authors.initials: B
authors.lastname: Liu
authors.forename: Xinmin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D008168:Lung / Q000473:pathology; D008297:Male; D009752:Nutritional Status; D059748:Proteolysis; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D012132:Respiratory Muscles / Q000378:metabolism*; D062789:Tobacco Products; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D014409:Tumor Necrosis Factor-alpha