title: In vitro ultraviolet A irradiation decreases both release ability and gene-expression of vascular endothelial growth factor-A from mast cells.
abstract: Ultraviolet A (UVA) rays penetrate the dermis, influencing the function of different cells, including mast cells, able to produce angiogenic factors. We investigated vascular endothelial growth factor (VEGF) release and gene-expression from mast cells (MCs), after UVA irradiation in vitro. The release of VEGF-A by Human MCs-1 (HMC-1) was induced by calcium ionophore A23187 and phorbol 12 myristate 13 acetate (PMA). Half of the cells received increasing doses of UVA (5, 25 and 50 J/cm(2)), the unirradiated HMC-1 served as controls. VEGF release and VEGF's messenger ribonucleic acid (mRNA) were detected respectively by enzyme-linked immunoabsorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) analysis. Results showed a UVA dose-dependent inhibitory effect on VEGF-A release from HMC-1. In particular, the release ability was reduced by 71.2% with 5 J/cm(2); 85% with 25 J/cm(2) and 86.3% with 50 J/cm(2). The VEGF-A RNA expression was reduced after UVA irradiation with 5 J/cm(2). We speculated that, at least in vitro and at our experimental conditions, UVA irradiation decreases mast cells-VEGF release and gene-expression.
authors.affiliation: Division of Allergy and Clinical Immunology, University of Naples Federico II, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Delfino
authors.forename: Luisa
authors.initials: L
authors.lastname: Di Costanzo
authors.forename: Amato
authors.initials: A
authors.lastname: de Paulis
authors.forename: Gabriella
authors.initials: G
authors.lastname: Fabbrocini
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Monfrecola
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D061207:Calcium Ionophores / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects*; D006801:Humans; D008407:Mast Cells; D012333:RNA, Messenger / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014466:Ultraviolet Rays; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*
chemical_list: D061207:Calcium Ionophores; D002273:Carcinogens; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate