title: Characterization of three pheromone-binding proteins (PBPs) of Helicoverpa armigera (Hübner) and their binding properties.
abstract: Three pheromone-binding proteins of Helicoverpa armigera were cloned and expressed in Escherichia coli. In order to characterize their physiological properties, ligand-binding experiments were performed using five biologically relevant substances including sex pheromones and interspecific signals. The results showed that one of the pheromone-binding proteins, HarmPBP1, binds strongly to each of the two principal pheromone components of H. armigera, (Z)-11-tetradecenal and (Z)-9-hexadecenal, but not to the interspecific signal (Z)-9-tetracecenal. The two remaining pheromone-binding proteins, HarmPBP2 and HarmPBP3, showed only weak affinities with the ligands tested. The 3-D structure of HarmPBP1 was predicted and the docking experiments indicate that the key binding site of (Z)-9-hexadecenal to HarmPBP1 includes Thr112, Lys111, and Phe119 whereas that of (Z)-11-tetradecenal includes Ser9, Trp37, Phe36, and Phe119.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Tian-Tao
authors.initials: TT
authors.lastname: Zhang
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Mei
authors.forename: Ji-Nian
authors.initials: JN
authors.lastname: Feng
authors.forename: Bente G
authors.initials: BG
authors.lastname: Berg
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Yu-Yuan
authors.initials: YY
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010675:Pheromones / Q000378:metabolism*; D011485:Protein Binding; D016415:Sequence Alignment
chemical_list: D002352:Carrier Proteins; D019476:Insect Proteins; D010675:Pheromones