title: Increased inducible heat shock protein 72 expression associated with PBMC isolated from patients with haematological tumours.
abstract: BACKGROUND
  Heat shock protein 72 (Hsp72) is a highly inducible stress protein and molecular chaperone. Cancers have been shown to be associated with increased Hsp72 expression within the tumour itself and this may lead to resistance to apoptosis.
  
  
  METHODS
  Peripheral blood mononuclear cells (PBMC) were isolated from patients diagnosed with chronic lymphocytic leukaemia (CLL) (n = 27) and chronic myelomonocytic leukaemia (CMML) (n = 16) and Hsp72 expression was characterized on both the cell surface and intracellularly by flow cytometry. To allow for comparison PBMC from breast cancer patients (n = 25) and healthy volunteers (n = 19) were included.
  
  
  RESULTS
  Both lymphocytes and monocytes from CLL and CMML patients showed high levels of total Hsp72 expression (4-6 fold increase) in comparison to breast cancer and healthy subjects. The majority of Hsp72 in these tumours was determined to be cell-surface expressed (64-93% of cell total Hsp72).
  
  
  CONCLUSIONS
  A correlation was observed between lymphocyte and monocyte total Hsp72 expression (p < 0.001) suggesting a common stress response pathway may exist in these blood cells and there are stress conditions present within the circulation. Hsp72 expression was not found to be related to white blood cell count.
authors.affiliation: Postgraduate Medical Institute, University of Hull, Hull, UK. l.a.madden@hull.ac.uk
authors.forename: Leigh A
authors.initials: LA
authors.lastname: Madden
authors.forename: Yvette A
authors.initials: YA
authors.lastname: Hayman
authors.forename: Corrinna
authors.initials: C
authors.lastname: Underwood
authors.forename: Rebecca V
authors.initials: RV
authors.lastname: Vince
authors.forename: John
authors.initials: J
authors.lastname: Greenman
authors.forename: David
authors.initials: D
authors.lastname: Allsup
authors.forename: Sahra
authors.initials: S
authors.lastname: Ali
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D001943:Breast Neoplasms / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D050884:HSP72 Heat-Shock Proteins / Q000097:blood*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood*; D015477:Leukemia, Myelomonocytic, Chronic / Q000097:blood* / Q000401:mortality; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D012189:Retrospective Studies; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D050884:HSP72 Heat-Shock Proteins; D008565:Membrane Proteins