title: Essential role of mast cells in the visceral hyperalgesia induced by T. spiralis infection and stress in rats.
abstract: Mast cells (MCs) deficient rats (Ws/Ws) were used to investigate the roles of MCs in visceral hyperalgesia. Ws/Ws and wild control (+/+) rats were exposed to T. spiralis or submitted to acute cold restraint stress (ACRS). Levels of proteinase-activated receptor 2 (PAR2) and nerve growth factor (NGF) were determined by immunoblots and RT-PCR analysis, and the putative signal pathways including phosphorylated extracellular-regulated kinase (pERK1/2) and transient receptor potential vanilloid receptor 1 (TRPV1) were further identified. Visceral hyperalgesia triggered by ACRS was observed only in +/+ rats. The increased expression of PAR2 and NGF was observed only in +/+ rats induced by T. spiralis and ACRS. The activation of pERK1/2 induced by ACRS occurred only in +/+ rats. However, a significant increase of TRPV1 induced by T. spiralis and ACRS was observed only in +/+ rats. The activation of PAR2 and NGF via both TRPV1 and pERK1/2 signal pathway is dependent on MCs in ACRS-induced visceral hyperalgesia rats.
authors.affiliation: Department of Gastroenterology, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, China.
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Yang
authors.forename: Yan-Yu
authors.initials: YY
authors.lastname: Wei
authors.forename: Chan-Juan
authors.initials: CJ
authors.lastname: Zhong
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Duan
mesh_terms: D000818:Animals; D003080:Cold Temperature; D003092:Colitis / Q000469:parasitology*; D003106:Colon / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006930:Hyperalgesia / Q000378:metabolism / Q000469:parasitology*; D008297:Male; D008407:Mast Cells / Q000166:cytology* / Q000469:parasitology*; D008856:Microscopy, Fluorescence / Q000379:methods; D020932:Nerve Growth Factor / Q000378:metabolism; D010766:Phosphorylation; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D055647:Rats, Transgenic; D044464:Receptor, PAR-2 / Q000378:metabolism; D015398:Signal Transduction; D017160:Trichinella spiralis / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D044464:Receptor, PAR-2; D020932:Nerve Growth Factor; D048049:Extracellular Signal-Regulated MAP Kinases