title: [Abnormal expression of microRNA-124 in patients with leukemia or myelodysplastic syndrome and its significance].
abstract: This study was aimed to investigate the abnormal expression of microRNA-124 (miR-124) in bone marrow cells of patients with leukemia or myelodysplastic syndrome (MDS) and its significance. The relative expression levels of miR-124 in bone marrow mononuclear cells from 33 patients with newly diagnosed leukemia or MDS, and 10 normal donors (as controls) were detected by stem-loop fluorescence real-time quantitative RT-PCR. The methylation levels of miR-124 promoter were detected by quantitative methylation specific PCR in partial MDS samples. The results indicated that as compared with normal control, lower levels of miR-124 (≤ 1/3) were found in 2/18 of leukemia patients and in 5/15 of MDS patients (among them ≤ 1/4 in 3/15 MDS patients). No statistically significance difference was observed between leukemia patients and normal controls (P = 0.725). However the difference was statistically significant between MDS group and control group (P = 0.031). Furthermore, an elevated methylation level of miR-124 promoter region in some of MDS patients (7/11) was detected by using quantitative methylation-specific PCR. The expression level of miR-124 was related with methylation level of promoter region (R(2) = 0.339, P = 0.018). It is concluded that the expression of miR-124 in partial MDS patients is inhibited, which may be associated with the abnormal methylation of its promoter.
authors.affiliation: Department of Hematology, Shanghai East Hospital, Tongji University, Shanghai, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Xia
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Yue-Sheng
authors.initials: YS
authors.lastname: Meng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D019175:DNA Methylation; D005260:Female; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D055815:Young Adult
chemical_list: C509207:MIRN124 microRNA, human; D035683:MicroRNAs