title: Feed-back regulation of disabled-2 (Dab2) p96 isoform for GATA-4 during differentiation of F9 cells.
abstract: F9 embryonic carcinoma (EC) cells undergo extra-embryonic endodermal (ExE) differentiation in response to retinoic acid (RA) treatment, which induces the expression of two isoforms (p96 and p67) of the adaptor protein, Disabled-2 (Dab2). In the current study, constitutive and ectopic expression of the p96 isoform induced ExE differentiation in F9 EC cells in the absence of RA treatment via the activation of GATA-4 by p96. During the RA-induced differentiation process, Dab2 expression is induced by the GATA factors in a coherent feed-forward loop; on the other hand, we showed that p96 regulates GATA-4 in a positive feed-back manner in this study. Our results indicate that p96 Dab2 plays a key role in the ExE differentiation process.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Jung Ah
authors.initials: JA
authors.lastname: Kim
authors.forename: Seong Ho
authors.initials: SH
authors.lastname: Bae
authors.forename: Young Joon
authors.initials: YJ
authors.lastname: Choi
authors.forename: Kyung Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Sung Soo
authors.initials: SS
authors.lastname: Park
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D018236:Carcinoma, Embryonal / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D045744:Cell Line, Tumor; D046508:Culture Techniques; D025461:Feedback, Physiological; D050991:GATA4 Transcription Factor / Q000378:metabolism*; D051379:Mice; D034741:RNA, Small Interfering / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D051017:Apoptosis Regulatory Proteins; C478773:Dab2 protein, mouse; D050991:GATA4 Transcription Factor; D034741:RNA, Small Interfering; D014212:Tretinoin