title: Clinical and pathological insights into Johne's disease in buffaloes.
abstract: Alternative diagnostic tools and interesting epidemiological assumptions were associated with an outbreak of Johne's disease. In a buffalo herd infected with paratuberculosis, seven clinically affected animals and 21 animals with anti-Mycobacterium avium ELISA reactions were identified. Total herd included 203 buffaloes. Most lesions were comparable to those described in buffaloes and cattle affected by Johne's disease. Water buffalo behaviors such as communal nursing and allosuckling may be additional risk factors for this disease. Detection of positive Ziehl-Neelsen staining and anti-M. avium immunolabeling in rectal biopsies from one buffalo with paratuberculosis are highlighted as auxiliary diagnostic tools for Johne's disease in live animals.
authors.affiliation: Setor de Patologia Veterinária, Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, n° 9090, Bairro Agronomia, CEP 91540-000 Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: André Cabrera
authors.initials: AC
authors.lastname: Dalto
authors.forename: Paulo Mota
authors.initials: PM
authors.lastname: Bandarra
authors.forename: Saulo Petinatti
authors.initials: SP
authors.lastname: Pavarini
authors.forename: Fabiana Marques
authors.initials: FM
authors.lastname: Boabaid
authors.forename: Ana Paula Gobbi
authors.initials: AP
authors.lastname: de Bitencourt
authors.forename: Marcos Pereira
authors.initials: MP
authors.lastname: Gomes
authors.forename: José
authors.initials: J
authors.lastname: Chies
authors.forename: David
authors.initials: D
authors.lastname: Driemeier
authors.forename: Cláudio Estêvão Farias
authors.initials: CE
authors.lastname: da Cruz
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001938:Brazil / Q000453:epidemiology; D002020:Buffaloes; D004196:Disease Outbreaks / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012007:Rectum / Q000382:microbiology / Q000473:pathology*; D012307:Risk Factors; D012621:Seasons
chemical_list: D000907:Antibodies, Bacterial