title: [Impact of clopidogrel on gene profile of human umbilical vein endothelial cell line and bioinformatics analysis].
abstract: This study was purposed to investigate the effect of clopidogrel on gene expression profile of cultured human umbilical vein endothelial cell (HUVEC) line (EA.hy926), and explore its potential molecule mechanism. A Affymetrix U133 plus 2.0 oligonucleotide microarray was applied to detect the alteration of gene expression profile induced by clopidogrel in HUVEC. Real time RT-PCR was used to verify the result of selected differentially expressing genes. The results showed that total 508 genes (including 139 up-regulated and 369 down-regulated genes) were obtained with differential expression more than 1.5-fold after clopidogrel (10 µmol/L) acted on HUVEC for 48 h. Clopidogrel affected the expression levels of genes involved protein binding, transcription factor activity, zinc ion binding, regulation of DNA-dependent transcription, transcription, RNA splicing and so on. It is concluded that the clopidogrel modulate function of endothelial cells by regulating sets of genes through different pathway.
authors.affiliation: Department of Geriatric Hematology, General Hospital of Chinese PLA, Beijing, China.
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Liu
authors.forename: Xue-Chun
authors.initials: XC
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Cong
authors.initials: C
authors.lastname: Ma
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
mesh_terms: D002460:Cell Line; D000077144:Clopidogrel; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D013988:Ticlopidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014158:Transcription, Genetic; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger; D000077144:Clopidogrel; D013988:Ticlopidine