title: Altered nucleotide cofactor-dependent properties of the mutant [S240K]RecA protein.
abstract: Two mutant Escherichia coli RecA proteins were prepared in which the ATP active site residue, Ser240, was replaced with asparagine and lysine (these amino acids are found in the corresponding positions in other bacterial RecA proteins). The S240N mutation had no discernible effect on the ATP-dependent activities of the RecA protein, indicating that serine and asparagine are functionally interchangeable at position 240. The S240K mutation, in contrast, essentially eliminated the ability of the RecA protein to utilize ATP as a nucleotide cofactor. The [S240K]RecA protein was able to catalyze the hydrolysis of dATP, however, suggesting that the absence of the 2'-hydroxyl group reduced an inhibitory interaction with the Lys240 side chain. Interestingly, the [S240K]RecA protein was able to promote an efficient LexA cleavage reaction but exhibited no strand exchange activity when dATP was provided as the nucleotide cofactor. This apparent separation of function may be attributable to the elevated S(0.5) value for dATP for the [S240K]RecA protein (490 μM, compared to 20-30 μM for the wild type and [S240N]RecA proteins), and may reflect a differential dependence of the LexA co-protease and DNA strand exchange activities on the nucleotide cofactor-mediated stabilization of the functionally-active state of the RecA-ssDNA complex.
authors.affiliation: Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21205, United States.
authors.forename: Scott E
authors.initials: SE
authors.lastname: Steffen
authors.forename: Floyd R
authors.initials: FR
authors.lastname: Bryant
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000378:metabolism; D003838:Deoxyadenine Nucleotides / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D006868:Hydrolysis; D008239:Lysine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009711:Nucleotides / Q000378:metabolism*; D059748:Proteolysis; D011938:Rec A Recombinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012694:Serine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D000099160:LexA Repressor Protein
chemical_list: D000255:Adenosine Triphosphate; D001426:Bacterial Proteins; D003838:Deoxyadenine Nucleotides; D008239:Lysine; D009711:Nucleotides; D011938:Rec A Recombinases; D012694:Serine; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein; C026600:2'-deoxyadenosine triphosphate