title: Mouse model for efficacy testing of antituberculosis agents via intrapulmonary delivery.
abstract: Here we describe an experimental murine model that allows for aerosolized antituberculosis drug efficacy testing. Intrapulmonary aerosol delivery of isoniazid, capreomycin, and amikacin to mice with pulmonary infection of Mycobacterium tuberculosis demonstrated efficacy in reducing pulmonary bacterial loads similar to that seen by standard drug delivery methods, even when lower concentrations of drugs and fewer doses were used in the aerosolized drug regimens. Interestingly, intrapulmonary delivery of isoniazid also reduced the bacterial load in the spleen.
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Gonzalez-Juarrero
authors.forename: Lisa K
authors.initials: LK
authors.lastname: Woolhiser
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Brooks
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: DeGroote
authors.forename: Anne J
authors.initials: AJ
authors.lastname: Lenaerts
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D000995:Antitubercular Agents / Q000008:administration & dosage* / Q000627:therapeutic use*; D005260:Female; D007538:Isoniazid / Q000008:administration & dosage* / Q000627:therapeutic use*; D008168:Lung / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000472:pathogenicity; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy*
chemical_list: D000995:Antitubercular Agents; D007538:Isoniazid