title: Dynamin II involves in cell migration and actin formation of NIH3T3 cells.
abstract: It was previously reported that in Ras transformed NIH3T3 cells, dynamin II acts as an intermediate messenger in the Ras signal transduction pathway leading to membrane ruffling and cell migration. However, these results do not provide sufficient evidence of a relationship between dynamin II and the Ras signal transduction pathway leading to membrane ruffling and cell migration. The results showed that a dynamin II association with myosin II as a signaling molecule is involved in NIH3T3 cell migration through the Ras/PI3K signaling pathway, and is associated with the p85 subunit of PI3K. Confocal microscopy also revealed co-localization between dynamin II and paxillin after PDGF stimulation. In addition, immunofluorescence results showed that dynamin II was colocalized with the actin filament. After stimulating the NIH3T3 cells with PDGF and treating them with an actin inhibitor, such as Cytochalasin D, it was observed that dynamin II with the myosin II complex inhibited binding to the actin. Therefore, dynamin II is localized in focal adhesion when cell migration is triggered and binds to the actin filament component, suggesting that it is a good candidate nanomolecule to regulate the cell attachment and migration to the materials such as implants etc.
authors.affiliation: Department of Oral Histology & Developmental Biology, School of Dentistry, Chosun University, Gwangju 501-759, Korea.
authors.forename: Do-Seon
authors.initials: DS
authors.lastname: Lim
authors.forename: Sang-Ryul
authors.initials: SR
authors.lastname: Kim
authors.forename: Baik-Dong
authors.initials: BD
authors.lastname: Choi
authors.forename: Soon-Jeong
authors.initials: SJ
authors.lastname: Jeong
authors.forename: Guanlin
authors.initials: G
authors.lastname: Wang
authors.forename: Hak-Kyun
authors.initials: HK
authors.lastname: Kim
authors.forename: Byung-Hoon
authors.initials: BH
authors.lastname: Kim
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Goo
authors.forename: Moon-Jin
authors.initials: MJ
authors.lastname: Jeong
mesh_terms: D000199:Actins / Q000096:biosynthesis*; D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D003599:Cytoskeleton / Q000502:physiology*; D034285:Dynamin II / Q000502:physiology*; D051379:Mice; D020409:Molecular Motor Proteins; D041681:NIH 3T3 Cells
chemical_list: D000199:Actins; D020409:Molecular Motor Proteins; D034285:Dynamin II