title: Inheritance and fine mapping of a restorer-of-fertility (Rf) gene for the cytoplasmic male sterility in soybean.
abstract: The cytoplasmic male sterility (CMS) line FuCMS5A and its restorer line FuHui9 were crossed to produce a segregating F(2) population for pollen fertility assay and the genetic mapping of restorer-of-fertility (Rf) gene. Results showed that the individual F(2) plants were fertile or semi-fertile based on their pollen fertility characteristics. The average ratios of viable pollen were 96.90% and 50.00% for each class of individuals. The segregation of F(2) plants showed a good fit to a 1:1 ratio, which reflects a typical heredity pattern of gametophytic CMS with fertility restorer being controlled by a single dominant gene. Using bulk segregation analysis (BSA) and genetic mapping, the Rf gene was mapped on molecular linkage group J (chromosome 16), between the simple sequence repeat (SSR) makers BARCSOYSSR-16-1064 and BARCSOYSSR-16-1082 with the distances of 0.59 and 0.83 cM, respectively. Four SSR markers (BARCSOYSSR-16-1070, Sctt011, BARCSOYSSR-16-1076 and BARCSOYSSR-16-1077) were cosegregating with this Rf gene in the mapping population. These makers will greatly facilitate the maker assisted selection procedures in CMS breeding programs and it lays a foundation for further map-base cloning of the Rf gene.
authors.affiliation: Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, PR China.
authors.forename: D K
authors.initials: DK
authors.lastname: Dong
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: F J
authors.initials: FJ
authors.lastname: Yuan
authors.forename: S L
authors.initials: SL
authors.lastname: Zhu
authors.forename: P
authors.initials: P
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Yu
authors.forename: Q H
authors.initials: QH
authors.lastname: Yang
authors.forename: X J
authors.initials: XJ
authors.lastname: Fu
authors.forename: X M
authors.initials: XM
authors.lastname: Yu
authors.forename: B Q
authors.initials: BQ
authors.lastname: Li
authors.forename: D H
authors.initials: DH
authors.lastname: Zhu
mesh_terms: D001947:Breeding; D002874:Chromosome Mapping; D003593:Cytoplasm / Q000235:genetics* / Q000502:physiology; D035264:Flowers / Q000235:genetics / Q000502:physiology; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D018895:Microsatellite Repeats; D051479:Plant Infertility / Q000235:genetics*; D011058:Pollen / Q000235:genetics / Q000502:physiology; D013025:Glycine max / Q000235:genetics* / Q000502:physiology
chemical_list: D005819:Genetic Markers