title: Identification and characterization of an aβ oligomer precipitating peptide that may be useful to explore gene therapeutic approaches to Alzheimer disease.
abstract: A key feature of Alzheimer disease (AD) is the pathologic self-association of the amyloid-β (Aβ) peptide, leading to the formation of diffusible toxic Aβ oligomers and extracellular amyloid plaques. Next to extracellular Aβ, intraneuronal Aβ has important pathological functions in AD. Agents that specifically interfere with the oligomerization processes either outside or inside of neurons are highly desired for the elucidation of the pathologic mechanisms of AD and might even pave the way for new AD gene therapeutic approaches. Here, we characterize the Aβ binding peptide L3 and its influence on Aβ oligomerization in vitro. Preliminary studies in cell culture demonstrate that stably expressed L3 reduces cell toxicity of externally added Aβ in neuroblastoma cells.
authors.affiliation: Institut of Complex Systems (ICS-6), Forschungszentrum Jülich, Jülich, Germany.
authors.forename: Susanne Aileen
authors.initials: SA
authors.lastname: Funke
authors.forename: Hongmei
authors.initials: H
authors.lastname: Liu
authors.forename: Torsten
authors.initials: T
authors.lastname: Sehl
authors.forename: Dirk
authors.initials: D
authors.lastname: Bartnik
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Brener
authors.forename: Luitgard
authors.initials: L
authors.lastname: Nagel-Steger
authors.forename: Katja
authors.initials: K
authors.lastname: Wiesehan
authors.forename: Dieter
authors.initials: D
authors.lastname: Willbold
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics* / Q000628:therapy*; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D000818:Animals; D052160:Benzothiazoles; D045744:Cell Line, Tumor; D002499:Centrifugation, Density Gradient; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D051379:Mice; D009391:Nephelometry and Turbidimetry; D009474:Neurons / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D017422:Sequence Analysis, DNA; D013844:Thiazoles / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D010455:Peptides; D013844:Thiazoles; C009462:thioflavin T