title: Biomedical evaluation of cortisol, cortisone, and corticosterone along with testosterone and epitestosterone applying micellar electrokinetic chromatography.
abstract: The validated micellar electrokinetic chromatography (MEKC) was proposed for the determination of five steroid hormones in human urine samples. That technique allowed for the separation and quantification of cortisol, cortisone, corticosterone, testosterone, and epitestosterone and was sensitive enough to detect low concentrations of these searched steroids in urine samples at the range of 2-300 ng/mL. The proposed MEKC technique with solid-phase extraction (SPE) procedure was simple, rapid, and has been successfully applied as a routine procedure to analyze steroids in human urine samples. The MEKC method offered a potential in clinical routine practice because of the short analysis time (8 min), low costs, and simultaneous analysis of five endogenous hormones. Due to its simplicity, speed, accuracy, and high recovery, the proposed method could offer a tool to determine steroid hormones as potential biomarkers in biomedical investigations, what was additionally revealed with healthy volunteers.
authors.affiliation: Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland. tbaczek@amg.gda.pl
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bączek
authors.forename: Ilona
authors.initials: I
authors.lastname: Olędzka
authors.forename: Lucyna
authors.initials: L
authors.lastname: Konieczna
authors.forename: Piotr
authors.initials: P
authors.lastname: Kowalski
authors.forename: Alina
authors.initials: A
authors.lastname: Plenis
mesh_terms: D000328:Adult; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003345:Corticosterone / Q000652:urine*; D003348:Cortisone / Q000652:urine*; D004845:Epitestosterone / Q000652:urine*; D006801:Humans; D006854:Hydrocortisone / Q000652:urine*; D008297:Male; D013739:Testosterone / Q000652:urine*
chemical_list: D013739:Testosterone; D004845:Epitestosterone; D003348:Cortisone; D003345:Corticosterone; D006854:Hydrocortisone