title: Biosynthesis of piperazic acid via N5-hydroxy-ornithine in Kutzneria spp. 744.
abstract: Which came first? We have investigated the biosynthesis of the piperazic acid (Piz) building blocks in the kutzneride family of metabolites. The flavin-dependent oxygenase KtzI was shown to convert ornithine to N(5)-OH-Orn. LC-MS/MS showed (13)C(5)-labeled versions of these two amino acids to be direct precursors of piperazic acid in vivo.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Neumann
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: John R
authors.initials: JR
authors.lastname: Heemstra
authors.forename: Erin A
authors.initials: EA
authors.lastname: Gontang
authors.forename: Roberto
authors.initials: R
authors.lastname: Kolter
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Walsh
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000378:metabolism*; D000596:Amino Acids / Q000737:chemistry; D009952:Ornithine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D011724:Pyridazines / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000596:Amino Acids; D011724:Pyridazines; C014756:piperazic acid; C004976:delta-N-hydroxyornithine; D009952:Ornithine