title: A stable panel comprising 18 urinary proteins in the human healthy population.
abstract: Just as biomarkers specific for diseases, biomarkers indicative of healthy conditions are valuable for the early diagnosis, monitoring, and prognosis of diseases. Our study focused on discovering via proteomics a stable panel of urinary proteins in the human healthy population. Urine samples were collected three times during 4 months from 100 male and 100 female healthy donors and analyzed through four different fractionation techniques (i.e. in-gel, 2D-LC, OFFGEL, and mRP) coupled with HPLC-Chip-MS/MS. Thus, 1641 urinary proteins were identified with a high confidence, among which 70 exhibiting an intergender/day variation <0.25 were selected and matched with the previously published five largest urinary proteomes to get 56 candidate proteins. Next, a panel comprising 18 intact urinary proteins was constructed by comparing the urinary proteomes via SDS-PAGE and 2DE. Finally, such 18 urinary proteins were validated via enzyme-linked immunosorbent assay in eight healthy individuals. Most of these proteins had been related to multiple rather than to single diseases. Therefore, we surmise that this protein set could be used as a biomarker to assess the human health status. Further determinations of the normal fluctuations of the single urinary proteins in this series using samples from large numbers of healthy individuals are required prior to any application in clinical settings.
authors.affiliation: Chongqing Key Laboratory for Disease Proteomics, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: He
authors.forename: Chibing
authors.initials: C
authors.lastname: Huang
authors.forename: Gaoxing
authors.initials: G
authors.lastname: Luo
authors.forename: Ilaria
authors.initials: I
authors.lastname: Dal Prà
authors.forename: Jiayu
authors.initials: J
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiwei
authors.initials: X
authors.lastname: Chen
authors.forename: Jiangling
authors.initials: J
authors.lastname: Tan
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Zhang
authors.forename: Ubaldo
authors.initials: U
authors.lastname: Armato
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D000704:Analysis of Variance; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D011507:Proteinuria / Q000652:urine*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D055815:Young Adult
chemical_list: D015415:Biomarkers; D020543:Proteome