title: PBN fails to suppress in delayed neuronal death of hippocampal CA1 injury following transient forebrain ischemia in gerbils.
abstract: Free radicals have been suggested to be involved in the genesis of ischemic brain damage, as shown by the protective effects of alpha-phenyl-N-tert-butyl nitrone (PBN), a spin trapping agent, in ischemic cerebral injury. However, the involvement of free radicals in transient ischemic-induced delayed neuronal death is not fully understood. To clarify this, in the present study, we evaluated the effect of PBN on delayed neuronal death and on the levels of free radicals in hippocampal CA1 region in the gerbil. The administration of PBN (10 mg/kg, i.v.) failed to show any preventive effect on the delayed neuronal death, examined by hematoxylin and eosin staining and the TUNEL method. Furthermore, we observed no free radical formation in delayed neuronal death, determined immunohistochemically using a specific 8-OHdG antibody, after transient ischemic insult. These results suggest that free radical formation may not contribute to the formation of delayed neuronal death.
authors.affiliation: Medical Science Division, United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Japan.
authors.forename: Wael
authors.initials: W
authors.lastname: Goda
authors.forename: Kunio
authors.initials: K
authors.lastname: Satoh
authors.forename: Masaya
authors.initials: M
authors.lastname: Nakashima
authors.forename: Akira
authors.initials: A
authors.lastname: Hara
authors.forename: Masayuki
authors.initials: M
authors.lastname: Niwa
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000473:pathology; D016923:Cell Death / Q000187:drug effects; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D005849:Gerbillinae; D002546:Ischemic Attack, Transient / Q000188:drug therapy*; D008297:Male; D015427:Reperfusion Injury / Q000188:drug therapy
chemical_list: D003497:Cyclic N-Oxides; D005609:Free Radicals; C029217:phenyl-N-tert-butylnitrone