title: In vitro antiplasmodial activity of spiro benzofuran compound from mangrove plant of Southern India.
abstract: OBJECTIVE
  To find out the in vitro antiplasmodial activities of mangrove leaf extracts.
  
  
  METHODS
  In vitro antiplasmodial assay was carried out with 13 different mangrove plants. Column chromatography was performed with the most potent Agecerious corniculatum (A. corniculatum) by using various solvent extractions. GC-MS was also preformed with the most potent ethanolic fraction of the A. corniculatum extract.
  
  
  RESULTS
  Of the 13 mangroves plants, A. corniculatum showed maximum percentage of parasitemia suppression (94.98 ± 1.16)%. Column chromatography was performed with A. corniculatum with different solvents and the methanolic extract showed maximum percentage (99.73±1.63)% of parasitemia inhibition at 150 μg/mL concentration with the IC(50) value of (29.28±3.23) μg/mL concentration. The results of the GC-MS analysis observed that, the most potent methanolic extract showed maximum retention time (30.687 RT) and the chemical class was identified as Spiro [benzofuran-2(3 H), 1'(3 cyclohexane)-2',3-dione, 7-chloro-4',6] which was responsible for the antiplasmodial activity.
  
  
  CONCLUSIONS
  It is concluded from the present study that, the chemical constituents of A. corniculatum collected from Pichavaram mangrove forest can be used as a putative antiplasmodial drugs in future.
authors.affiliation: School of Marine Sciences, Department of Oceanography and Coastal Area Studies, Alagappa University, Thondi Campus, Thondi, Ramanathapuram District, Tamil Nadu, India. ravibiotech201321@gmail.com
authors.forename: Ravikumar
authors.initials: R
authors.lastname: Sundaram
authors.forename: Ramanathan
authors.initials: R
authors.lastname: Ganesan
authors.forename: Gnanadesigan
authors.initials: G
authors.lastname: Murugesan
mesh_terms: D000962:Antimalarials / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D002845:Chromatography; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D016778:Malaria, Falciparum / Q000188:drug therapy; D018512:Parasitemia / Q000188:drug therapy; D021261:Parasitic Sensitivity Tests / Q000379:methods; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D010963:Plasmodium falciparum / Q000187:drug effects*; D029623:Primulaceae
chemical_list: D000962:Antimalarials; D001572:Benzofurans; D010936:Plant Extracts