title: Process technology for multi-enzymatic reaction systems.
abstract: In recent years, biocatalysis has started to provide an important green tool in synthetic organic chemistry. Currently, the idea of using multi-enzymatic systems for industrial production of chemical compounds becomes increasingly attractive. Recent examples demonstrate the potential of enzymatic synthesis and fermentation as an alternative to chemical-catalysis for the production of pharmaceuticals and fine chemicals. In particular, the use of multiple enzymes is of special interest. However, many challenges remain in the scale-up of a multi-enzymatic system. This review summarizes and discusses the technology options and strategies that are available for the development of multi-enzymatic processes. Some engineering tools, including kinetic models and operating windows, for developing and evaluating such processes are also introduced.
authors.affiliation: Center for Process Engineering and Technology, Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Rui
authors.initials: R
authors.lastname: Xue
authors.forename: John M
authors.initials: JM
authors.lastname: Woodley
mesh_terms: D055162:Biocatalysis; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D004798:Enzymes / Q000378:metabolism*; D008954:Models, Biological
chemical_list: D004798:Enzymes