title: Nitrergic response to Clostridium perfringens infection in the rat brain regions: effect of red light irradiation.
abstract: A single intraperitoneal injection of a gram-positive pathogen Clostridium perfringens (Cp) causes a remarkable down-regulation the constitutive nitric oxide synthase (cNOS) with a simultaneous increase in the activity of inducible NOS (iNOS) and the level of reactive nitrogen species in the rat brain major regions (cortex, striatum, hippocampus and hypothalamus) at 48 h post-administration of Cp. Treatment by both a semiconductor laser (SCL) and/or a light-emitting diode (LED) with same wavelength, energy density and time exposure (continuous wave, λ=654 nm, fluence=1.27 J/cm(2), time exposure=600 s) could modulate brain nitrergic response following Cp-infection. Besides, unlike the LED, the SCL-irradiation prevents the cNOS inhibition in all the studied brain regions and might be useful in restoring its function in neurotransmission and cerebral blood flow, along with providing a protective effect against nitrosative stress-induced iNOS-mediated injury in the brain regions.
authors.affiliation: H.Buniatian Institute of Biochemistry NAS RA, 5/1 P. Sevak St., 0014, Yerevan, Republic of Armenia.
authors.forename: H A
authors.initials: HA
authors.lastname: Movsesyan
authors.forename: N Kh
authors.initials: NKh
authors.lastname: Alchujyan
authors.forename: N H
authors.initials: NH
authors.lastname: Movsesyan
authors.forename: A G
authors.initials: AG
authors.lastname: Guevorkian
authors.forename: H L
authors.initials: HL
authors.lastname: Hairapetyan
authors.forename: K A
authors.initials: KA
authors.lastname: Barsegyan
authors.forename: G A
authors.initials: GA
authors.lastname: Kevorkian
mesh_terms: D000818:Animals; D003015:Clostridium Infections / Q000201:enzymology / Q000532:radiotherapy*; D003016:Clostridium perfringens / Q000528:radiation effects*; D007031:Hypothalamus / Q000201:enzymology / Q000382:microbiology / Q000528:radiation effects*; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D008297:Male; D026602:Nitrergic Neurons / Q000378:metabolism / Q000528:radiation effects*; D052248:Nitric Oxide Synthase Type I / Q000009:adverse effects / Q000037:antagonists & inhibitors / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D026361:Reactive Nitrogen Species / Q000096:biosynthesis / Q000528:radiation effects; D016896:Treatment Outcome
chemical_list: D026361:Reactive Nitrogen Species; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II