title: Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus.
abstract: OBJECTIVE
  To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection.
  
  
  METHODS
  We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.
  
  
  RESULTS
  The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus.
  
  
  CONCLUSIONS
  This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.
authors.affiliation: Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Piyathida
authors.initials: P
authors.lastname: Pongsiri
authors.forename: Kesmanee
authors.initials: K
authors.lastname: Praianantathavorn
authors.forename: Apiradee
authors.initials: A
authors.lastname: Theamboonlers
authors.forename: Sunchai
authors.initials: S
authors.lastname: Payungporn
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D018354:Alphavirus Infections / Q000175:diagnosis*; D065632:Chikungunya Fever; D002646:Chikungunya virus / Q000302:isolation & purification*; D015342:DNA Probes; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes