title: Production and in vitro antioxidant activity of exopolysaccharide by a mutant, Cordyceps militaris SU5-08.
abstract: Cordyceps militaris SU5-08 was derived from an initial strain (C. militaris SU5) by ultraviolet mutagenesis of protoplasts, and the extraction parameters for C. militaris SU5-08 exopolysaccharide (EPS) produced during submerged culture were optimized. The extraction rate of EPS was 1919.16±165.27 mg/l, which was 120.38±11.36% higher than that of C. militaris SU5. The in vitro scavenging effects of EPS of C. militaris SU5-08 on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals at a dosage of 5 g/l were 63.64±3.52%, 75.27±5.16%, and 6.46±5.03%, respectively. The reducing power of EPS of C. militaris SU5-08 was 0.21±0.01. The results suggest that the EPS of C. militaris SU5-08 can be used as a potential antioxidant which enhances adaptive immune responses.
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Rongshan
authors.initials: R
authors.lastname: Lin
authors.forename: Honghong
authors.initials: H
authors.lastname: Liu
authors.forename: Suqian
authors.initials: S
authors.lastname: Wu
authors.forename: Lifei
authors.initials: L
authors.lastname: Pang
authors.forename: Mengshi
authors.initials: M
authors.lastname: Jia
authors.forename: Keming
authors.initials: K
authors.lastname: Fan
authors.forename: Shouhua
authors.initials: S
authors.lastname: Jia
authors.forename: Le
authors.initials: L
authors.lastname: Jia
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D032661:Cordyceps / Q000235:genetics / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism; D009154:Mutation; D010084:Oxidation-Reduction / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D013481:Superoxides; D017665:Hydroxyl Radical