title: The effect of c-Fos demethylation on sodium fluoride-induced apoptosis in L-02 cells.
abstract: To investigate the effects of sodium fluoride (NaF) on apoptosis, c-Fos mRNA and protein expression levels, and methylation status as well as Dnmt1, Dnmt3a, and Dnmt3b mRNA expression levels in human embryo hepatocyte (L-02) which were exposed to different concentrations of NaF (0, 20, 40, and 80 mg/l) for 24 h in vitro. Results showed that the percentage of apoptosis and c-Fos mRNA and protein expression levels in 40 and 80 mg/l NaF-treated groups were higher than those in the control group (P<0.05). Further, Dnmt1 mRNA expression level was significantly decreased in the 80 mg/l NaF-treated groups compared to the control group (P<0.05); Dnmt3a and Dnmt3b mRNA expression levels were significantly decreased in 40 and 80 mg/l NaF-treated groups compared to the control group (P<0.05). c-Fos methylation levels, according to the bisulfite sequencing results, were decreased in 20, 40, and 80 mg/l NaF-treated groups against the control group. These results suggest that NaF could induce apoptosis and upregulate mRNA and protein expression level of c-Fos as well as decrease mRNA expression levels of Dnmt1, Dnmt3a, and Dnmt3b in L-02 cells. The decrease in c-Fos methylation levels might be involved in the early phase of apoptosis induced by NaF in L-02 cells.
authors.affiliation: Department of Environmental Health and MOE key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Niu
authors.forename: HongLiang
authors.initials: H
authors.lastname: Liu
authors.forename: ZhiZhong
authors.initials: Z
authors.lastname: Guan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zeng
authors.forename: ShuXia
authors.initials: S
authors.lastname: Guo
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.forename: LiJuan
authors.initials: L
authors.lastname: Guo
authors.forename: Ping
authors.initials: P
authors.lastname: Gao
authors.forename: BaYi
authors.initials: B
authors.lastname: Xu
authors.forename: ZhiXia
authors.initials: Z
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Xia
authors.forename: AiGuo
authors.initials: A
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018899:CpG Islands; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D000090205:DNA Methyltransferase 3A; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D012969:Sodium Fluoride / Q000009:adverse effects*; D000097584:DNA Methyltransferase 3B
chemical_list: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D012969:Sodium Fluoride; D000097584:DNA Methyltransferase 3B; C000717967:DNMT3A protein, human; C000619380:DNMT1 protein, human