title: Determination of trace indium in urine after preconcentration with a chelating-resin-packed minicolumn.
abstract: A simple and rapid chelating-resin-packed column has been developed for preconcentration of trace indium in biological samples. A large-sized urine sample was pumped through a minicolumn at a flow rate of 1.0 mL/min by using a peristaltic pump, and the eluents were analyzed using graphite furnace atomic absorption spectrometry (GFAAS). Four commercially available chelating resins including Chelex-100, Amberlite IRC-50, Duolite GT-73, and Celite 545-AW were studied for evaluating the indium sorption performance. Several parameters, such as pH, resin amount, eluent volume, eluent flow rate, and the volume of sample, were investigated and optimized. A 100-200 mL of the sample was loaded into a column containing 1.2 g of wet Chelex-100 and subjected to the ion-exchange procedure. The retained analytes were eluted with 5.0 mL of 0.1 M HNO(3)  and quantified by GFAAS. The correlation coefficient in the range 10-250 ng/mL was of 0.9994. The limit of detection of the proposed method was 2.75 ng/mL. The method developed was successfully applied to analysis of spiked urine samples with good recoveries of 93-103% (n = 6) and reproducibility (relative standard deviation < 4.9%). The accuracy of procedure was confirmed by indium determination in spiked certified reference materials.
authors.affiliation: Department of Safety, Health and Environmental Engineering, Hungkuang University, Taichung Hsien, Taichung, Taiwan, R.O.C. hmliu@sunrise.hk.edu.tw
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Liu
authors.forename: Ching-Yao
authors.initials: CY
authors.lastname: Chang
authors.forename: Chia-Chan
authors.initials: CC
authors.lastname: Wu
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Wei
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Chen
authors.forename: Cheng-Tsung
authors.initials: CT
authors.lastname: Yeh
mesh_terms: D000327:Adsorption; D000328:Adult; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D007204:Indium / Q000302:isolation & purification / Q000652:urine*; D008297:Male; D012117:Resins, Synthetic / Q000737:chemistry*; D013054:Spectrophotometry, Atomic; D055815:Young Adult
chemical_list: D012117:Resins, Synthetic; D007204:Indium