title: Distinctive heat-shock response of bioleaching microorganism  Acidithiobacillus ferrooxidans  observed using genome-wide microarray.
abstract: Temperature plays an important role in the heap bioleaching. The maldistribution of ventilation in the heap leads to local hyperthermia, which does exert a tremendous stress on bioleaching microbes. In this study, the genome-wide expression profiles of  Acidithiobacillus ferrooxidans  at 40 °C were detected using the microarray. The results showed that some classic proteases like Lon and small heat-shock proteins were not induced, and heat-inducible membrane proteins were suggested to be under the control of σ(E). Moreover, expression changes of energy metabolism are noteworthy, which is different from that in heterotrophic bacteria upon heat stress. The induced enzymes catalyzed the central carbon metabolism pathway that might mainly provide precursors of amino acids for protein synthesis. These results will deepen the understanding of the mechanisms of heat-shock response on autotrophic bacteria.
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083 Hunan, China.
authors.forename: Huaqun
authors.initials: H
authors.lastname: Yin
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhou
authors.forename: Xian
authors.initials: X
authors.lastname: Fu
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Yili
authors.initials: Y
authors.lastname: Liang
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.forename: Xueduan
authors.initials: X
authors.lastname: Liu
mesh_terms: D042763:Acidithiobacillus / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D016680:Genome, Bacterial; D050888:Heat-Shock Proteins, Small / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D051877:RNA Polymerase Sigma 54 / Q000378:metabolism; D012808:Sigma Factor / Q000378:metabolism; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D050888:Heat-Shock Proteins, Small; D012808:Sigma Factor; C063202:sporulation-specific sigma factors; D002244:Carbon; D051877:RNA Polymerase Sigma 54