title: Sediminihabitans luteus gen. nov., sp. nov., a new member of the family Cellulomonadaceae isolated from sea sediment.
abstract: Two novel Gram-positive actinobacteria, designated H97-3(T) and H83-5, were isolated from marine sediment samples and their taxonomic positions were investigated by a polyphasic approach. Both strains formed vegetative hyphae in the early phase of growth but the hyphae eventually fragmented into coccoid cells. The peptidoglycan type was found to be A4α. The predominant menaquinone was MK-9(H(4)), and the major fatty acids were anteiso-C(15:0), anteiso-C(17:0) and C(16:0). The DNA G+C content was 74.0-74.9 mol %. 16S rRNA gene sequencing analysis revealed that strains H97-3(T) and H83-5 represented novel members of the family Cellulomonadaceae. Their nearest phylogenetic neighbours were the members of the genus Oerskovia, with a similarity of 98.3-98.4 %. However, strains H97-3(T) and H83-5 were distinguishable from the members of the genus Oerskovia and the other genera of the family Cellulomonadaceae in terms of chemotaxonomic characteristics and phylogenetic relationship. The result of the DNA-DNA hybridization indicated that strains H97-3(T) and H83-5 belonged to the same species. Therefore, strains H97-3(T) and H83-5 represent a novel genus and species of the family Cellulomonadaceae, for which the name Sediminihabitans luteus gen. nov., sp. nov. is proposed. The type strain of S. lutes is H97-3(T) (=NBRC 108568(T) = DSM 25478(T)).
authors.affiliation: NITE Biological Resource Center, National Institute of Technology and Evaluation, Kisarazu, Chiba, Japan. hamada-moriyuki@nite.go.jp
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.forename: Chiyo
authors.initials: C
authors.lastname: Shibata
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamura
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hayakawa
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D005227:Fatty Acids / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D005227:Fatty Acids