title: Visualization of candidate division OP3 cocci in limonene-degrading methanogenic cultures.
abstract: Members of candidate division OP3 were detected in 16S rRNA gene clone libraries from methanogenic enrichment cultures that utilized limonene as a carbon and energy source. We developed probes for the visualization of OP3 cells. In situ hybridization experiments with newly designed OP3-specific probes [OP3-565 and Eub-338(VI)] revealed abundant small OP3 cocci attached to larger cells. Syntrophic Deltaproteobacteria, OP3 cells, and methanogens affiliating with Methanoculleus and Methanosaeta formed the limonenedegrading community.
authors.affiliation: Department of Microbiology, Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D-28359 Bremen, Germany.
authors.forename: Amelia-Elena
authors.initials: AE
authors.lastname: Rotaru
authors.forename: Regina
authors.initials: R
authors.lastname: Schauer
authors.forename: Christina
authors.initials: C
authors.lastname: Probian
authors.forename: Marc
authors.initials: M
authors.lastname: Mussmann
authors.forename: Jens
authors.initials: J
authors.lastname: Harder
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D053138:Cyclohexenes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D000077222:Limonene; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013729:Terpenes / Q000378:metabolism*
chemical_list: D053138:Cyclohexenes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D013729:Terpenes; D000077222:Limonene; D008697:Methane