title: A novel HPLC method for the concurrent analysis and quantitation of seven water-soluble vitamins in biological fluids (plasma and urine): a validation study and application.
abstract: An HPLC method was developed and validated for the concurrent detection and quantitation of seven water-soluble vitamins (C, B(1), B(2), B(5), B(6), B(9), B(12)) in biological matrices (plasma and urine). Separation was achieved at 30°C on a reversed-phase C18-A column using combined isocratic and linear gradient elution with a mobile phase consisting of 0.01% TFA aqueous and 100% methanol. Total run time was 35 minutes. Detection was performed with diode array set at 280 nm. Each vitamin was quantitatively determined at its maximum wavelength. Spectral comparison was used for peak identification in real samples (24 plasma and urine samples from abstinent alcohol-dependent males). Interday and intraday precision were <4% and <7%, respectively, for all vitamins. Recovery percentages ranged from 93% to 100%.
authors.affiliation: Centre for Health Services Studies, University of Kent, Canterbury, Kent CT2 7NF, UK. mg209@kent.ac.uk
authors.forename: Margherita Grotzkyj
authors.initials: MG
authors.lastname: Giorgi
authors.forename: Kevin
authors.initials: K
authors.lastname: Howland
authors.forename: Colin
authors.initials: C
authors.lastname: Martin
authors.forename: Adrian B
authors.initials: AB
authors.lastname: Bonner
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014815:Vitamins / Q000097:blood* / Q000652:urine*; D014867:Water
chemical_list: D014815:Vitamins; D014867:Water