title: Royal jelly modulates oxidative stress and tissue injury in gamma irradiated male Wister Albino rats.
abstract: BACKGROUND
  Royal jelly is a nutritive secretion produced by the worker bees, rich in proteins, carbohydrates, vitamins and minerals.
  
  
  OBJECTIVE
  The present study was designed to determine the possible protective effects of royal jelly against radiation induced oxidative stress, hematological, biochemical and histological alterations in male Wister albino rats.
  
  
  METHODS
  Male Wister albino rats were exposed to a fractionated dose of gamma radiation (2 Gy every 3 days up to 8 Gy total doses). Royal jelly was administrated (g/Kg/day) by gavages 14 days before exposure to the 1(st) radiation fraction and the treatment was continued for 15 days after the 1(st) irradiation fraction till the end of the experiment. The rats were sacrificed 3(rd), equivalent to 3rd post 2nd irradiation fraction, and equivalent to 3rd day post last irradiation fraction.
  
  
  RESULTS
  In the present study, gamma- irradiation induced hematological, biochemical and histological effects in male Wister albino rats. In royal jelly treated irradiated group, there was a noticeable decrease recorded in thiobarbituric reactive substances concentration when compared to γ-irradiated group. Also, the serum nitric oxide concentration was significantly improved. The administration of royal jelly to irradiated rats according to the current experimental design significantly ameliorates the changes induced in serum lipid profile. Moreover, in royal jelly treated irradiated group, there was a noticeable amelioration recorded in all hematological parameters along the three experimental intervals. The microscopic examination of cardiac muscle of royal jelly treated irradiated rats demonstrated structural amelioration, improved nuclei and normal features of capillaries and veins in endomysium when compared to gamma-irradiated rats.
  
  
  CONCLUSIONS
  It was suggested that the biochemical, hematological and histological amelioration observed in royal jelly (g/Kg/day) treated irradiated rats might be due to the antioxidant capacity of royal jelly active constituents.
authors.affiliation: Department of Radiation Biology, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
authors.forename: Khaled Shaaban
authors.initials: KS
authors.lastname: Azab
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bashandy
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Salem
authors.forename: Osama
authors.initials: O
authors.lastname: Ahmed
authors.forename: Zaki
authors.initials: Z
authors.lastname: Tawfik
authors.forename: Hamed
authors.initials: H
authors.lastname: Helal