title: Development and characterization of microsatellite markers for Berberis thunbergii (Berberidaceae).
abstract: OBJECTIVE
  Microsatellite markers were isolated and characterized in Berberis thunbergii, an invasive and ornamental shrub in the eastern United States, to assess genetic diversity among populations and potentially identify horticultural cultivars.
  
  
  RESULTS
  A total of 12 loci were identified for the species. Eight of the loci were polymorphic and were screened in 24 individuals from two native (Tochigi and Ibaraki prefectures, Japan) and one invasive (Connecticut, USA) population and 21 horticultural cultivars. The number of alleles per locus ranged from three to seven, and observed heterozygosity ranged from 0.048 to 0.636.
  
  
  CONCLUSIONS
  These new markers will provide tools for examining genetic relatedness of B. thunbergii plants in the native and invasive range, including phylogeographic studies and assessment of rapid evolution in the invasive range. These markers may also provide tools for examining hybridization with other related species in the invasive range.
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT 06269, USA. jenica.allen@uconn.edu
authors.forename: Jenica M
authors.initials: JM
authors.lastname: Allen
authors.forename: Samuel G
authors.initials: SG
authors.lastname: Obae
authors.forename: Mark H
authors.initials: MH
authors.lastname: Brand
authors.forename: John A
authors.initials: JA
authors.lastname: Silander
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Jones
authors.forename: Schyler O
authors.initials: SO
authors.lastname: Nunziata
authors.forename: Stacey L
authors.initials: SL
authors.lastname: Lance
mesh_terms: D028142:Berberis / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism; D058865:Introduced Species; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers