title: AtSUC2 has a role for sucrose retrieval along the phloem pathway: evidence from carbon-11 tracer studies.
abstract: The location of the phloem within a plant, and its vulnerability to disruption, make it a difficult tissue to study and therefore non-invasive studies of phloem functionality are important. Here we compare, phloem transport, measured non-invasively, in wild type Arabidopsis thaliana, and transposon-insertion mutants for AtSUC1 or AtSUC2, giving in vivo information on the importance of these sucrose transporters for phloem transport. The suc2 mutant showed an increase in both phloem leakage and transport time, consistent with reduced sucrose uptake into both transport and collection phloem. The results are consistent with the AtSUC2 transporter being important for retrieval of leaked sucrose in the transport phloem of Arabidopsis. There was no difference in phloem transport properties between the wild type and the suc1 mutants, implying that the AtSUC1 transporter does not play a significant role within the transport phloem of Arabidopsis under the conditions of our study.
authors.affiliation: The New Zealand Institute for Plant & Food Research Limited, Private Bag 3230, Waikato Mail Centre, Hamilton 3240, New Zealand. nick.gould@plantandfood.co.nz
authors.forename: N
authors.initials: N
authors.lastname: Gould
authors.forename: M R
authors.initials: MR
authors.lastname: Thorpe
authors.forename: J
authors.initials: J
authors.lastname: Pritchard
authors.forename: J T
authors.initials: JT
authors.lastname: Christeller
authors.forename: L E
authors.initials: LE
authors.lastname: Williams
authors.forename: G
authors.initials: G
authors.lastname: Roeb
authors.forename: U
authors.initials: U
authors.lastname: Schurr
authors.forename: P E H
authors.initials: PE
authors.lastname: Minchin
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002244:Carbon / Q000378:metabolism; D002250:Carbon Radioisotopes; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D055786:Gene Knockout Techniques; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D052585:Phloem / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D026901:Membrane Transport Proteins; D010940:Plant Proteins; C078729:sucrose transport protein, plant; D013395:Sucrose; D002244:Carbon