title: Factors influencing extract of Hibiscus sabdariffa staining of rat testes.
abstract: Some plant extracts can be used in biology and medicine to reveal or identify cellular components and tissues. We investigated the effects of time and concentration on staining of histological sections of rat testes by an acidified extract of Hibiscus sabdariffa. An ethanolic extract of H. sabdariffa was diluted using 1% acetic acid in 70% ethanol to stain histological sections of testes at concentrations of 0.2, 0.1 and 0.05 g/ml for 5, 10, 15, 30, 45 and 60 min. The sections of testes were stained deep red. The staining efficiency of H. sabdariffa was greater at a high concentration and required less time to achieve optimal staining. H. sabdariffa is a strongly basic dye that can be used for various diagnostic purposes. Staining time and concentration must be considered to achieve optimal results.
authors.affiliation: Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo, Akwa Ibom State, Nigeria. rosemary_bassey@yahoo.com
authors.forename: R B
authors.initials: RB
authors.lastname: Bassey
authors.forename: A A
authors.initials: AA
authors.lastname: Bakare
authors.forename: A I
authors.initials: AI
authors.lastname: Peter
authors.forename: A A
authors.initials: AA
authors.lastname: Oremosu
authors.forename: A A
authors.initials: AA
authors.lastname: Osinubi
mesh_terms: D000818:Animals; D031584:Hibiscus / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*; D013737:Testis / Q000737:chemistry*; D013997:Time Factors
chemical_list: D010936:Plant Extracts