title: Sphingobium cupriresistens sp. nov., a copper-resistant bacterium isolated from copper mine soil, and emended description of the genus Sphingobium.
abstract: A gram-negative, aerobic, copper-resistant bacterium, designated strain CU4(T), was isolated from copper mine soil in Daye, China. Phylogenetic analysis based on 16S rRNA gene sequences showed highest similarity to Sphingobium rhizovicinum CC-FH12-1(T) (98.4 %), followed by Sphingobium francense Sp+(T) (97.2 %), Sphingobium japonicum UT26(T) (97.1 %), Sphingobium abikonense NBRC 16140(T) (97.0 %), Sphingobium xenophagum DSM 6383(T) (96.9 %) and Sphingobium yanoikuyae DSM 7462(T) (95.5 %). The major fatty acids (>5 %) were summed feature 7 (C(18 : 1)ω7c, C(18 : 1)ω9t and/or C(18 : 1)ω12t), summed feature 4 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH), C(16 : 0) and C(14 : 0) 2-OH, and the predominant quinone was ubiquinone Q-10. Spermidine was the major polyamine component. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidyldimethylethanolamine and phosphatidylcholine. The genomic DNA G+C content of strain CU4(T) was 64.9 mol%. Comparison of DNA-DNA hybridization, phenotypic and chemotaxonomic characteristics between strain CU4(T) and phylogenetically related strains revealed that the new isolate represents a novel species of the genus Sphingobium, for which the name Sphingobium cupriresistens sp. nov. is proposed. The type strain is CU4(T) ( = KCTC 23865(T) = CCTCC AB 2011146(T)). An emended description of the genus Sphingobium is also proposed.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Liqiong
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Zunji
authors.initials: Z
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Gejiao
authors.initials: G
authors.lastname: Wang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D003300:Copper; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008906:Mining; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D003300:Copper; C025204:Ubiquinone Q2