title: Characterization of cerebrospinal fluid aminoterminally truncated and oxidized amyloid-β peptides.
abstract: OBJECTIVE
  Carboxyterminally elongated and aminoterminally truncated Aβ peptides as well as their pyroglutamate and oxidized derivates are major constituents of human amyloid plaques. The objective of the present study was to characterize aminoterminally truncated or oxidized Aβ38, Aβ40, and Aβ42 peptide species in immunoprecipitated human cerebrospinal fluid (CSF).
  
  
  METHODS
  We invented a novel sequential aminoterminally and carboxyterminally specific immunoprecipitation protocol and used the Aβ-SDS-PAGE/immunoblot for subsequent analysis of CSF Aβ peptide patterns.
  
  
  RESULTS
  In the present study, we identified the aminoterminally truncated Aβ peptides 2-40 and 2-42 as well as oxidized forms of Aβ1-38 and Aβ1-42 in CSF. Our protocol allowed the quantification of a pattern of Aβ peptides 1-38(ox), 2-40, and 2-42 in addition to the well known panel of Aβ 1-37, 1-38, 1-39, 1-40, 1-40(ox), and 1-42 in a group of seven patients with peripheral polyneuropathy.
  
  
  CONCLUSIONS
  In the present approach, we could broaden the range of quantifiable Aβ peptides described in previous studies (i.e., 1-37, 1-38, 1-39, 1-40, 1-40(ox), and 1-42) by Aβ 1-38(ox), 2-40, and 2-42. An exact analysis of CSF Aβ peptides regarding their carboxy- and aminoterminus as well as posttranslational modification seems promising with respect to diagnostic and pathogenic aspects.
authors.affiliation: Department of Psychiatry, Psychotherapy and Addiction Medicine, Kliniken Essen-Mitte, University of Duisburg-Essen, Essen, Germany. m.bibl@kliniken-essen-mitte.de
authors.forename: Mirko
authors.initials: M
authors.lastname: Bibl
authors.forename: Marion
authors.initials: M
authors.lastname: Gallus
authors.forename: Volker
authors.initials: V
authors.lastname: Welge
authors.forename: Sabine
authors.initials: S
authors.lastname: Lehmann
authors.forename: Katrin
authors.initials: K
authors.lastname: Sparbier
authors.forename: Hermann
authors.initials: H
authors.lastname: Esselmann
authors.forename: Jens
authors.initials: J
authors.lastname: Wiltfang
mesh_terms: D000368:Aged; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid* / Q000378:metabolism; D015415:Biomarkers / Q000134:cerebrospinal fluid / Q000378:metabolism; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation / Q000379:methods*; D008875:Middle Aged; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000134:cerebrospinal fluid* / Q000378:metabolism; D010523:Peripheral Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D016229:Amyloid beta-Peptides; D015415:Biomarkers; D010446:Peptide Fragments; C078639:amyloid beta-protein (1-38); C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42); C442220:amyloid beta-protein (2-42)