title: The effect of the juvenile hormone analog, fenoxycarb, on ecdysone receptor B1 expression in the midgut of Bombyx mori during larval-pupal metamorphosis.
abstract: The Bombyx mori (Lepidoptera: Bombycidae) midgut undergoes remodeling during the larval-pupal metamorphosis. All metamorphic events in insects are controlled by mainly two hormones: 20-hydroxyecdysone (20E) and juvenile hormone (JH). Fenoxycarb, O-ethyl N-(2-(4-phenoxyphenoxy)-ethyl) carbamate, has been shown to be one of the most potent juvenile hormone analogs against a variety of insect species. In this study, the effect of fenoxycarb on EcR-B1 protein expression in the midgut of Bombyx mori during the remodeling processwas investigated. Fenoxycarb was topically treated to the beginning of the fifth instar Bombyx larvae. Its application prolonged the last instar and prevented metamorphic events. Analyses were performed from day 6 of the fifth instar to 24 hr after pupation in controls and to day 14 of the fifth instar in the fenoxycarb treated group. According to our results, the presence of EcR-B1 in the midguts of the fenoxycarb treated group during the feeding period suggested that EcR-B1 was involved in the functioning of larval cells and during this period fenoxycarb did not affect EcR-B1 status. Immediately after termination of the feeding stage, the amount of EcR-B1 protein increased, which indicated that it may strengthen the ecdysone signal for commitment of remodeling process. In the fenoxycarb treated group, its upregulation was delayed, which may be related to the inhibition of ecdysone secretion from the prothoracic gland.
authors.affiliation: Department of Biology, Ege University, Faculty of Science, 35100 Bornova-Izmir, Turkey. ebru.goncu@ege.edu.tr
authors.forename: Ebru
authors.initials: E
authors.lastname: Goncu
authors.forename: Osman
authors.initials: O
authors.lastname: Parlak
mesh_terms: D000818:Animals; D012831:Bombyx / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D016923:Cell Death; D007814:Larva / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008675:Metamorphosis, Biological; D048448:Phenylcarbamates / Q000494:pharmacology*; D011679:Pupa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011987:Receptors, Steroid / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D048448:Phenylcarbamates; D011987:Receptors, Steroid; C055469:ecdysone receptor; C052034:fenoxycarb