title: Molecular cloning and characterization of a cyclophilin A homologue from Schistosoma japonicum.
abstract: Cyclophilins belong to a group of proteins that have peptidyl-prolyl cis-trans isomerase activity and have been identified in all cell types and all organisms studied. In both prokaryotes and eukaryotes, they have been characterized as functional chaperones and involved in cell signaling. In the present study, Sj cyclophilin A (CyPA) was cloned, characterized, and subcloned into a prokaryotic expression vector to produce soluble recombinant rSjCyPA protein. qPCR analysis revealed that SjCyPA was expressed at each schistosome developmental stage tested, but reached its highest levels at days 7 and 13. In addition, the gene was also found to be significantly downregulated in adult worms from Microtus fortis. The SjCyPA protein was located on the subtegumental musculature of Schistosoma japonicum as determined by immunohistochemical staining analysis. Direct administration of recombinant SjCyPA to mice induced partial protective efficacy against subsequent schistosome infection. Length and width of adult worms and expression of SjCyPA were significantly decreased in the immunized groups, at 42 days post-infection, indicating that immunization with recombinant SjCyPA may suppress the schistosomes development. rSjCyPA can also react with sera from S. japonicum-infected rabbits at different time points. The data presented here suggest that SjCyPA may be an important molecule in the schistosome life-cycle and may be useful as a therapeutic target to treat schistosomiasis infection or as a potential diagnostic antigen.
authors.affiliation: College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, Jiangsu 225009, China.
authors.forename: Hongxiao
authors.initials: H
authors.lastname: Han
authors.forename: Jinbiao
authors.initials: J
authors.lastname: Peng
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Fu
authors.forename: Jinjun
authors.initials: J
authors.lastname: Xu
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Lin
authors.forename: Jianping
authors.initials: J
authors.lastname: Tao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth; D000947:Antigens, Helminth / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular; D021984:Cyclophilin A / Q000737:chemistry*; D018076:DNA, Complementary; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000276:immunology* / Q000378:metabolism*; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010802:Phylogeny; D011817:Rabbits; D011897:Random Allocation; D011994:Recombinant Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology / Q000378:metabolism*; D012554:Schistosomiasis japonica / Q000517:prevention & control; D014612:Vaccines / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D015801:Helminth Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014612:Vaccines; D021984:Cyclophilin A