title: Effect of natural mediators on the stability of Trametes trogii laccase during the decolourization of textile wastewaters.
abstract: The purpose of the present study was to determine the effect of natural mediators on the stability of the Trametes trogii crude laccase in the process of decolourization of textile effluents. Acetosyringone allowed the highest wastewaters decolourization rate of 25%. At higher concentrations of acetosyringone, the relative activity of laccase decreased approximately by between 38% and 88% after 5 days of incubation. T. trogii laccase was strongly inactivated at 3 mM syringaldehyde, after 3 days of incubation. However, laccase activity is more stable in the presence of the vanillin and m-coumarate. The T. trogii growth on solid effluent-based-medium was examined and evaluated by measuring the colony diameter in cm. T. trogii was completely inhibited on 100:0 and 80:20 effluent:water solid medium, however, colony diameter reached 5 cm on 60:40 effluent:water solid medium after 13-14 days incubation. When the textile effluent was pre-treated with laccase and laccase-acetosyringone system, the colony diameter of 2 cm of T. trogii on 80:20 effluent:water solid medium was reached after 14 and 10 days of incubation respectively.
authors.affiliation: Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Sidi Mansour Road Km 6, PB 1177, 3018, Sfax, Tunisia. rimkhlifi@yahoo.fr
authors.forename: Rim
authors.initials: R
authors.lastname: Khlifi
authors.forename: Rim
authors.initials: R
authors.lastname: Khlifi-Slama
authors.forename: Tahar
authors.initials: T
authors.lastname: Mechichi
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
authors.forename: Abdelhafidh
authors.initials: A
authors.lastname: Dhouib
mesh_terms: D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D007220:Industrial Waste / Q000032:analysis; D007700:Kinetics; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D012722:Sewage / Q000032:analysis*; D013783:Textile Industry; D055454:Trametes / Q000737:chemistry / Q000201:enzymology*
chemical_list: D004396:Coloring Agents; D005656:Fungal Proteins; D007220:Industrial Waste; D012722:Sewage; D042845:Laccase