title: Ectopic overexpression of swine PPARγ2 upregulated adipocyte genes expression and triacylglycerol in skeletal muscle of mice.
abstract: Peroxisome proliferator-activated receptor γ2 (PPARγ2) is a key regulator of adipocyte differentiation, fatty acid uptake and storage in mammals. The primary goal of the present study was to investigate the consequences of PPARγ2 overexpression in the muscle. A swine muscle creatine kinase promoter was used to drive swine PPARγ2 (sPPARγ2) overexpression in the muscle of a transgenic mice model. The results showed that the mRNA of multiple adipocyte genes was increased in the skeletal muscle, as evidenced by the up-regulation of fatty acid synthase (2.11-fold, P < 0.05), lipoprotein lipase (2.08-fold, P < 0.01), fatty acid-binding protein 4 (14.30-fold, P < 0.01), and CD36 antigen (5.50-fold, P < 0.01). Meanwhile, skeletal muscle triacylglycerol was increased (P < 0.01) and the fatty acid profile of muscle fat was changed in that more polyunsaturated fats acid were augmented. The present study may further serve to develop transgenic pigs with higher intramuscular fat content and improved pork quality.
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Huang
authors.forename: Yuanzhu
authors.initials: Y
authors.lastname: Xiong
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Zijian
authors.initials: Z
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Zuo
authors.forename: Dequan
authors.initials: D
authors.lastname: Xu
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Ren
mesh_terms: D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D015153:Blotting, Western; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D005838:Genotype; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D050556:Fatty Acid-Binding Proteins; D005230:Fatty Acids, Nonesterified; D047495:PPAR gamma; D012333:RNA, Messenger; D014280:Triglycerides