title: Origins and formation of histone methylation across the human cell cycle.
abstract: The connections between various nuclear processes and specific histone posttranslational modifications are dependent to a large extent on the acquisition of those modifications after histone synthesis. The reestablishment of histone posttranslational modifications after S phase is especially critical for H3K9 and H3K27 trimethylation, both of which are linked with epigenetic memory and must be stably transmitted from one cellular generation to the next. This report uses a proteomic strategy to interrogate how and when the cell coordinates the formation of histone posttranslational modifications during division. Paramount among the findings is that H3K9 and H3K27 trimethylation begins during S phase but is completed only during the subsequent G(1) phase via two distinct pathways from the unmodified and preexisting dimethylated states. In short, we have systematically characterized the temporal origins and methylation pathways for histone posttranslational modifications during the cell cycle.
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
authors.forename: Barry M
authors.initials: BM
authors.lastname: Zee
authors.forename: Laura-Mae P
authors.initials: LM
authors.lastname: Britton
authors.forename: Daniel
authors.initials: D
authors.lastname: Wolle
authors.forename: Devorah M
authors.initials: DM
authors.lastname: Haberman
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Garcia
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D044127:Epigenesis, Genetic; D016193:G1 Phase / Q000235:genetics / Q000502:physiology; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008715:Methionine / Q000737:chemistry; D008745:Methylation; D008954:Models, Biological; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D016196:S Phase / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction
chemical_list: D017931:DNA Primers; D006657:Histones; D008715:Methionine