title: A novel approach to signal normalisation in atmospheric pressure ionisation mass spectrometry.
abstract: The aim of our study was to test an alternative principle of signal normalisation in LC-MS/MS. During analyses, post column infusion of the target analyte is done via a T-piece, generating an "area under the analyte peak" (AUP). The ratio of peak area to AUP is assessed as assay response. Acceptable analytical performance of this principle was found for an exemplary analyte. Post-column infusion may allow normalisation of ion suppression not requiring any additional standard compound. This approach can be useful in situations where no appropriate compound is available for classical internal standardisation.
authors.affiliation: Institute of Clinical Chemistry, University of Munich, Munich, Germany. michael.vogeser@med.uni-muenchen.de
authors.forename: Michael
authors.initials: M
authors.lastname: Vogeser
authors.forename: Fabian
authors.initials: F
authors.lastname: Kirchhoff
authors.forename: Roland
authors.initials: R
authors.lastname: Geyer
mesh_terms: D001274:Atmospheric Pressure; D002853:Chromatography, Liquid / Q000379:methods*; D003437:Crotonates / Q000032:analysis*; D006885:Hydroxybutyrates; D007166:Immunosuppressive Agents / Q000032:analysis; D009570:Nitriles; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014052:Toluidines / Q000032:analysis*
chemical_list: D003437:Crotonates; D006885:Hydroxybutyrates; D007166:Immunosuppressive Agents; D009570:Nitriles; D014052:Toluidines; C527525:teriflunomide