title: Development of real-time PCR for the detection of Clostridium perfringens in meats and vegetables.
abstract: A real-time PCR assay was developed and validated inhouse specifically for the detection of Clostridium perfringens (Cl. perfringens) in meats and vegetables by comparing with the culture method. The detection limit of the real-time PCR assay in phosphate-buffered saline was 10² CFU/ml. When the two methods were compared in food samples inoculated with Cl. perfringens, the culture method detected 52 positives, whereas real-time PCR detected 51 positives out of 160 samples. The difference was without statistical significance (p>0.05). Real-time PCR assay is an option for quality assurance laboratories to perform standard diagnostic tests, considering its detection ability and time-saving efficiency.
authors.affiliation: KU Center for Food Safety, Veterinary Science Research Institute and College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.
authors.forename: Jung-Whan
authors.initials: JW
authors.lastname: Chon
authors.forename: Jong-Seok
authors.initials: JS
authors.lastname: Park
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Hyeon
authors.forename: Chankyu
authors.initials: C
authors.lastname: Park
authors.forename: Kwang-Young
authors.initials: KY
authors.lastname: Song
authors.forename: Kwang-Won
authors.initials: KW
authors.lastname: Hong
authors.forename: In-Gyun
authors.initials: IG
authors.lastname: Hwang
authors.forename: Hyo-Sun
authors.initials: HS
authors.lastname: Kwak
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
mesh_terms: D000818:Animals; D002417:Cattle; D003016:Clostridium perfringens / Q000235:genetics / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013552:Swine; D014675:Vegetables / Q000737:chemistry / Q000382:microbiology*