title: The autophagy-associated factors DRAM1 and p62 regulate cell migration and invasion in glioblastoma stem cells.
abstract: The aggressiveness of glioblastoma multiforme (GBM) is defined by local invasion and resistance to therapy. Within established GBM, a subpopulation of tumor-initiating cells with stem-like properties (GBM stem cells, GSCs) is believed to underlie resistance to therapy. The metabolic pathway autophagy has been implicated in the regulation of survival in GBM. However, the status of autophagy in GBM and its role in the cancer stem cell fraction is currently unclear. We found that a number of autophagy regulators are highly expressed in GBM tumors carrying a mesenchymal signature, which defines aggressiveness and invasion, and are associated with components of the MAPK pathway. This autophagy signature included the autophagy-associated genes DRAM1 and SQSTM1, which encode a key regulator of selective autophagy, p62. High levels of DRAM1 were associated with shorter overall survival in GBM patients. In GSCs, DRAM1 and SQSTM1 expression correlated with activation of MAPK and expression of the mesenchymal marker c-MET. DRAM1 knockdown decreased p62 localization to autophagosomes and its autophagy-mediated degradation, thus suggesting a role for DRAM1 in p62-mediated autophagy. In contrast, autophagy induced by starvation or inhibition of mTOR/PI-3K was not affected by either DRAM1 or p62 downregulation. Functionally, DRAM1 and p62 regulate cell motility and invasion in GSCs. This was associated with alterations of energy metabolism, in particular reduced ATP and lactate levels. Taken together, these findings shed new light on the role of autophagy in GBM and reveal a novel function of the autophagy regulators DRAM1 and p62 in control of migration/invasion in cancer stem cells.
authors.affiliation: Samantha Dickson Brain Cancer Unit, UCL Cancer Institute, London, UK.
authors.forename: S
authors.initials: S
authors.lastname: Galavotti
authors.forename: S
authors.initials: S
authors.lastname: Bartesaghi
authors.forename: D
authors.initials: D
authors.lastname: Faccenda
authors.forename: M
authors.initials: M
authors.lastname: Shaked-Rabi
authors.forename: S
authors.initials: S
authors.lastname: Sanzone
authors.forename: A
authors.initials: A
authors.lastname: McEvoy
authors.forename: D
authors.initials: D
authors.lastname: Dinsdale
authors.forename: F
authors.initials: F
authors.lastname: Condorelli
authors.forename: S
authors.initials: S
authors.lastname: Brandner
authors.forename: M
authors.initials: M
authors.lastname: Campanella
authors.forename: R
authors.initials: R
authors.lastname: Grose
authors.forename: C
authors.initials: C
authors.lastname: Jones
authors.forename: P
authors.initials: P
authors.lastname: Salomoni
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D001343:Autophagy / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics*; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008565:Membrane Proteins / Q000502:physiology*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D000071456:Sequestosome-1 Protein; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C512709:DRAM1 protein, human; D008565:Membrane Proteins; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein