title: Proteomic analysis of proteins increased or reduced by ethanol of Lactobacillus plantarum ST4 isolated from Makgeolli, traditional Korean rice wine.
abstract: LAB were isolated from makgeolli locally produced around Jinju, Gyeongnam, S. Korea during spring of 2011. Randomly selected 11 isolates from MRS agar plates were identified first by API CHL 50 kits and then 16S rRNA gene sequencing. All 11 isolates were identified as Lactobacillus plantarum. Among them, ST4 grew in MRS broth with ethanol up to 10%, showing the highest alcohol resistance. L. plantarum ST4 was moderately resistant against acid and bile salts. When cellular proteins of L. plantarum ST4 under ethanol stress were analyzed by two-dimensional gel electrophoresis (2DE), the intensities of 6 spots increased, whereas 22 spots decreased at least 2-fold. Those 28 spots were identified by peptide mass fingerprinting (PMF). FusA2 (elongation factor G) increased 18.8-fold (6% ethanol) compared with control. Other proteins were AtpD (ATP synthase subunit beta), DnaK, GroEL, Tuf (elongation factor Tu), and Npr2 (NADH peroxidase), respectively. Among the 22 proteins decreased in intensities, lactate dehydrogenases (LdhD and LdhL1) were included.
authors.affiliation: Division of Applied Life Science (BK21), Graduate school, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Seung Gyu
authors.initials: SG
authors.lastname: Lee
authors.forename: Kang Wook
authors.initials: KW
authors.lastname: Lee
authors.forename: Tae Heung
authors.initials: TH
authors.lastname: Park
authors.forename: Ji Yeong
authors.initials: JY
authors.lastname: Park
authors.forename: Nam Soo
authors.initials: NS
authors.lastname: Han
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism / Q000382:microbiology*; D040901:Proteomics; D014920:Wine / Q000032:analysis / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D000431:Ethanol