title: Trichostatin A and ascorbic acid assist in the development of porcine handmade cloned embryos via different physiologic pathways.
abstract: The objective was to determine the effects of ascorbic acid (AA), trichostatin A (TSA), and their combined treatment (TA) on reprogramming and development of cloned porcine embryos. Embryos treated with AA (50 and 100 µg/mL) had a higher blastocyst rate than controls (49.6% and 44.0% vs 30.7%, P < .05). Blastocyst rates of handmade cloned (HMC) embryos were nearly 60% in both the 30 and 40 nmol/L TSA treatment groups, which were higher (P < .05) than the control (29.4%). The TA treatment groups had a higher blastocyst rate compared with the AA treatment alone (58.9% vs 43.5%, P < .05). Histone acetylation was much higher in the TSA and TA treatments (primarily in 2- and 4-celled embryos) but was not significantly different between AA-treated and untreated embryos. Both AA and TA treatments reduced apoptotic rates of blastocysts. In conclusion, AA supplementation improved blastocyst development in porcine HMC embryos mainly by a traditional antioxidant pathway rather than by cellular reprogramming.
authors.affiliation: Department of Animal Science, National Chung Hsing University, Taichung, Taiwan, Republic of China.
authors.forename: Siriboon
authors.initials: S
authors.lastname: Chawalit
authors.forename: Ngoc Tan
authors.initials: NT
authors.lastname: Nguyen
authors.forename: Jung-Kai
authors.initials: JK
authors.lastname: Tseng
authors.forename: Neng-Wen
authors.initials: NW
authors.lastname: Lo
authors.forename: Ching-Fu
authors.initials: CF
authors.lastname: Tu
authors.forename: Jyh-Cherng
authors.initials: JC
authors.lastname: Ju
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001205:Ascorbic Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods*; D046149:Embryo Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian / Q000187:drug effects / Q000196:embryology; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology*; D005260:Female; D006877:Hydroxamic Acids / Q000494:pharmacology*; D013552:Swine
chemical_list: D006877:Hydroxamic Acids; C012589:trichostatin A; D001205:Ascorbic Acid