title: [Use of real-time PCR for quantitative assessment of lactic acid bacteria and bifidobacteria in dairy products].
abstract: Composition of lactic acid bacteria and bifidobacteria in raw milk and home-made milk products has been analyzed using real-time PCR (quantitative PCR) with genus-specific primers to Enterococcus, Lactobacillus and Bifidobacteria. Bacteria belonging to these genera have been revealed in all samples analyzed (milk, sour cream, cottage cheese). It has been shown that the representatives of Enterococcus and Lactobacillus genera dominated in the samples analyzed (10(3)-10(7) genome equivalent/ml (mg)). The largest number of these microorganisms (10(7) genome equivalent/mg) has been detected in cottage cheese.
authors.forename: L B
authors.initials: LB
authors.lastname: Zelenaia
authors.forename: N K
authors.initials: NK
authors.lastname: Kovalenko
authors.forename: R V
authors.initials: RV
authors.lastname: Oblap
authors.forename: N B
authors.initials: NB
authors.lastname: Hovak
authors.forename: R A
authors.initials: RA
authors.lastname: Golubets
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000235:genetics* / Q000302:isolation & purification; D002138:Calibration; D002611:Cheese / Q000382:microbiology*; D015169:Colony Count, Microbial; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis*; D016983:Enterococcus / Q000235:genetics* / Q000302:isolation & purification; D005516:Food Microbiology; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000235:genetics* / Q000302:isolation & purification; D008892:Milk / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D019344:Lactic Acid