title: Flow injection chemiluminescence determination of 2-methoxyestradiol based on inhibition of luminol-potassium ferricyanide reaction.
abstract: A novel flow-injection chemiluminescence (FI-CL) method is described for the determination of 2-methoxyestradiol (2-ME). The method is based on the inhibitory effect of 2-ME on the CL reaction of luminol and potassium ferricyanide in alkaline solution. Under optimal conditions, net CL intensity was proportional to 2-ME concentration in synthetic and mouse plasma samples. Corresponding linear regression equations were 8.0 x 10(-9) -1.0 x 10(-7) g/mL for synthetic samples and 2.0 x 10(-9) -1.0 x 10(-7) g/mL for plasma samples. Detection limit for synthetic samples and limits for quantification of plasma samples were 8.4 x 10(-10) g/mL (3σ) for synthetic samples and 4.0 x 10(-9) g/mL for mouse samples. A complete analysis was performed for 60 s, including washing and sampling, resulting in a throughput of ≈ 60/h. The proposed method was applied for the determination of 2-ME in synthetic and mouse plasma samples. Percentage recoveries were 101.0-102.8% and 98.0-105.0%, respectively. A possible mechanism responsible for CL reaction is proposed.
authors.affiliation: School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, Henan, 450001, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Jin
authors.forename: Hanchun
authors.initials: H
authors.lastname: Yao
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Pang
authors.forename: Bin
authors.initials: B
authors.lastname: Du
mesh_terms: D000077584:2-Methoxyestradiol; D000818:Animals; D004958:Estradiol / Q000031:analogs & derivatives* / Q000097:blood; D005292:Ferricyanides / Q000037:antagonists & inhibitors / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D051379:Mice
chemical_list: D005292:Ferricyanides; D004958:Estradiol; D008165:Luminol; D000077584:2-Methoxyestradiol; C028033:potassium ferricyanide