title: Association of AFLP and SCAR markers with common  leafspot resistance in autotetraploid alfalfa (Medicago sativa).
abstract: To identify amplified fragment length polymorphism  (AFLP) markers associated with resistance or susceptibility of alfalfa to  common leafspot (CLS) caused by the fungus Pseudopeziza medicaginis (Dermateaceae),  bulked segregant analysis was conducted based on an F(1(M × M)) population of 93 plants and a BC(1)S population of 91 plants. Three AFLP markers, ACTCAA(R206), TAGCAC(R185),  and GGACTA(S264), were found to be associated with CLS  resistance or susceptibility. All three markers were found at significantly  different frequencies (71.9, 80.3 and 91.8%) compared to resistant or  susceptible plants in the original population. Subsequently, these three AFLP  markers were converted into three SCAR markers, ACTCAA(R136), TAGCAC(R128) and  GGACTA(S254), which are easier to employ in breeding  programs. The three SCAR markers were used in a randomly selected population  with 50% resistance; the probability of finding one resistant plant was  increased to 67.3, 66.7 and 90.0% with markers ACTCAA(R136), TAGCAC(R128) and  GGACTA(S254), independently. If two of the SCAR  markers were used simultaneously, the probability would be higher than 89%. The  three SCAR markers identified in this study would be applicable for selection  for CLS resistance in alfalfa breeding programs. Moreover, the genetic analysis  indicated that CLS resistance in alfalfa is conferred by a single dominant  gene.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Bi
authors.forename: Q H
authors.initials: QH
authors.lastname: Yuan
authors.forename: X L
authors.initials: XL
authors.lastname: Li
authors.forename: J M
authors.initials: JM
authors.lastname: Gao
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D017343:Genes, Plant; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D000455:Medicago sativa / Q000235:genetics* / Q000382:microbiology; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D015203:Reproducibility of Results
chemical_list: D005819:Genetic Markers