title: Do serum hepcidin-25 levels correlate with oxidative stress in patients with chronic kidney disease not receiving dialysis?
abstract: OBJECTIVE
  Iron metabolism is an important factor of anemia in chronic kidney disease (CKD). Hepcidin is a regulator of iron homeostasis and has a major role in the anemia of chronic disease (ACD). Oxidative stress (OS) is also associated with iron metabolism. However, the clinical utility of hepcidin, especially its association with OS, in CKD patients not receiving dialysis is still unclear.
  
  
  METHODS
  We recruited 117 patients (62 ± 15 years, 85 males, and median estimated glomerular filtration rate (eGFR) 22 ml/min/1.73 m2) with CKD not receiving dialysis. Serum 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker of DNA oxidative injury, and serum hepcidin-25 were measured by ELISA and by liquid chromatography tandem mass spectrometry, respectively.
  
  
  RESULTS
  Hepcidin-25 was associated positively with ferritin, high-sensitive C-reactive protein (hsCRP) and 8-OHdG, and negatively with eGFR and hemoglobin. Sex, oral iron, hemoglobin, transferrin saturation (TSAT), ferritin, and hsCRP were independently associated with hepcidin-25 in a multiple regression model. In contrast, neither eGFR nor 8-OHdG independently affected hepcidin- 25.
  
  
  CONCLUSIONS
  The close association between hepcidin and serum ferritin, oral iron and hsCRP indicates that it plays a key role in the pathogenesis of anemia in patients with CKD not receiving dialysis. In contrast, effects of eGFR and OS were not apparent.
authors.affiliation: Division of Kidney and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan. maruyama@td5.so-net.ne.jp
authors.forename: Yukio
authors.initials: Y
authors.lastname: Maruyama
authors.forename: Keitaro
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Masaaki
authors.initials: M
authors.lastname: Nakayama
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Hosoya
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D023181:Antimicrobial Cationic Peptides / Q000097:blood*; D002097:C-Reactive Protein / Q000032:analysis; D002908:Chronic Disease; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D004921:Erythropoietin / Q000627:therapeutic use; D005260:Female; D064451:Hepcidins; D006801:Humans; D007674:Kidney Diseases / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D006435:Renal Dialysis
chemical_list: D023181:Antimicrobial Cationic Peptides; D064451:Hepcidins; C545475:hepcidin 25, human; D004921:Erythropoietin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002097:C-Reactive Protein; D003849:Deoxyguanosine