title: Efficient production of L-lactic acid with high optical purity by alkaliphilic Bacillus sp. WL-S20.
abstract: Highly efficient polymer-grade L-lactic acid production was achieved by an alkaliphilic strain Bacillus sp. WL-S20 using inexpensive peanut meal as nitrogen source and sodium hydroxide as neutralizing agent. In multi-pulse fed-batch fermentation of Bacillus sp. WL-S20, a L-lactic acid concentration of 225 g/l with a yield of 99.3% was obtained. In single-pulse fed-batch fermentation, a concentration of 180 g/l was obtained with a yield of 98.6%. No D-isomers of lactic acid were detected. The production of a high concentration of optically pure L-lactic acid by alkaliphilic Bacillus sp. WL-S20, combined with a low-cost nutrient and environment-friendly NaOH-based process, represent a potentially novel way for L-lactic acid production at an industrial scale.
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Meng
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Xue
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Chenghua
authors.initials: C
authors.lastname: Gao
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D000468:Alkalies / Q000494:pharmacology*; D001407:Bacillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D019344:Lactic Acid / Q000096:biosynthesis*; D009584:Nitrogen / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D013696:Temperature
chemical_list: D000468:Alkalies; D019344:Lactic Acid; D002244:Carbon; D005947:Glucose; D009584:Nitrogen; D010100:Oxygen