title: Intranasal immunization with live attenuated influenza vaccine plus chitosan as an adjuvant protects mice against homologous and heterologous virus challenge.
abstract: Our previous studies have proven the adjuvanticity of chitosan in mice when administered with inactivated and subunit influenza vaccine. In this study, we investigated the adjuvant effect of chitosan on the immunogenicity and protective efficacy of a live attenuated influenza vaccine. Mice were inoculated intranasally with live attenuated influenza vaccine plus chitosan and then challenged with a high, lethal dose of homologous or heterologous virus. Antibody responses, secretion of IFN-γ by spleen cells, body weight loss, survival rates, and residual lung virus titers were tested. The results demonstrated that live attenuated influenza vaccine with chitosan adjuvant not only protected mice completely against challenge with the homologous virus but also provided good cross-protection against a heterologous virus. In addition, chitosan as adjuvant could significantly increase the levels of antigen-specific antibodies and the population of IFN-γ-secreting T cells. These results reveal the potential of chitosan as a candidate adjuvant for use in a live attenuated influenza vaccine.
authors.affiliation: Shanghai Institute of Biological Products, Shanghai 200052, China.
authors.forename: Xueliang
authors.initials: X
authors.lastname: Wang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.forename: Fen
authors.initials: F
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Zheng
authors.forename: Dan
authors.initials: D
authors.lastname: Zheng
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Yinglei
authors.initials: Y
authors.lastname: Yi
authors.forename: Yahong
authors.initials: Y
authors.lastname: Ding
authors.forename: Jian
authors.initials: J
authors.lastname: Luo
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Dai
authors.forename: Hanzhong
authors.initials: H
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Sun
authors.forename: Ze
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D048271:Chitosan / Q000276:immunology*; D056738:Cross Protection / Q000276:immunology; D005260:Female; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D007371:Interferon-gamma; D048271:Chitosan