title: Adipose tissue secretion and expression of adipocyte-produced and stromavascular fraction-produced adipokines vary during multiple phases of weight-reducing dietary intervention in obese women.
abstract: BACKGROUND
  Obesity is associated with altered plasma levels of adipokines involved in the development of insulin resistance and obesity-related metabolic disturbances.
  
  
  OBJECTIVE
  The aim was to investigate diet-induced changes in adipokine production in sc abdominal adipose tissue (SAT) during a 6-month, multiphase, weight-reducing dietary intervention.
  
  
  METHODS
  Forty-eight obese women followed a dietary intervention consisting of a very low-calorie diet (VLCD) (1 month), followed by a weight-stabilization (WS) period, which consisted of a low-calorie diet (2 months), and a weight-maintenance diet (3 months).
  
  
  METHODS
  Before and at the end of the VLCD and WS, samples of plasma and SAT were obtained. In a subgroup of 26 women, secretion of adipokines was determined in SAT explants, and in a subgroup of 22 women, SAT mRNA expression was measured.
  
  
  RESULTS
  Body weight decreased and insulin sensitivity increased during the intervention. Plasma levels, SAT mRNA expression, and secretion rates of adipocyte-produced adipokines (leptin, serum amyloid A, and haptoglobin) decreased during the VLCD and increased during the WS period. Adipokines produced mainly from stroma-vascular cells (IL-6, IL-8, IL-10, IL-1Ra, TNFα, plasminogen activator inhibitor-1, and monocyte chemoattractant protein-1) increased or remained unchanged during VLCD and decreased to levels equal to or lower than prediet levels during the WS period. The diet-induced changes in homeostasis model assessment of insulin resistance correlated with changes in leptin plasma levels during VLCD, WS, and the entire dietary intervention period.
  
  
  CONCLUSIONS
  Diet-induced regulation of adipokine production in SAT differs according to their cellular origin (adipocytes vs. stroma-vascular cells) and diet phase (VLCD vs. WS). Insulin-sensitivity changes were associated only with those of plasma leptin.
authors.affiliation: Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, CZ-100 00, Czech Republic. msiklova@email.cz
authors.forename: Michaela
authors.initials: M
authors.lastname: Siklova-Vitkova
authors.forename: Eva
authors.initials: E
authors.lastname: Klimcakova
authors.forename: Jan
authors.initials: J
authors.lastname: Polak
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kovacova
authors.forename: Michaela
authors.initials: M
authors.lastname: Tencerova
authors.forename: Lenka
authors.initials: L
authors.lastname: Rossmeislova
authors.forename: Magda
authors.initials: M
authors.lastname: Bajzova
authors.forename: Dominique
authors.initials: D
authors.lastname: Langin
authors.forename: Vladimir
authors.initials: V
authors.lastname: Stich
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D054392:Adipokines / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000328:Adult; D031204:Caloric Restriction; D002458:Cell Fractionation; D004038:Diet, Reducing; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000178:diet therapy* / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D055815:Young Adult
chemical_list: D054392:Adipokines