title: Antagonizing effects of soybean isoflavones on β-amyloid peptide-induced oxidative damage in neuron mitochondria of rats.
abstract: Soybean isoflavone (SIF) has been demonstrated to have neuroprotective effects induced by β-amyloid peptides (Aβ) through suppressing oxidative stress; however, the explicit mechanisms still remain uncovered. In the present study, 32 Wistar rats were randomly divided into four groups: an Aβ1-42-treated group, a SIF + Aβ1-42 group, a SIF-treated group and a control group. We measured the protein content of 8-hydroxydeoxyguanosine (8-OhdG) and mRNA expression of 8-oxoguanine DNA glycosylase (OGG1). The protein expression of OGG1, Bcl-xl, Bad, beta subunit of ATP synthase (ATPB) and pyruvate dehydrogenase (PDH) in brain was also measured. The results showed that the level of 8-OHdG in both SIF groups was significantly decreased compared to the Aβ1-42-treated group (p < 0.05), while the mRNA and protein expression of OGG1 in the SIF + Aβ1-42 groups were up-regulated compared with the Aβ1-42-treated groups (p < 0.05). The expression of Bcl-xl was up-regulated in the SIF-treated group compared with the Aβ1-42-treated groups (p < 0.05), while the expression of Bad was down-regulated in the two SIF-treated groups (p < 0.05). Aβ1-42 significantly down-regulated the expression of ATPase and PDH proteins compared with the control group (p < 0.05). SIF reversed the down-regulation effects on the mitochondrial energy metabolic enzymes induced by Aβ1-42 (p < 0.05) in the rats. These results suggest that SIF alleviate the oxidative stress in neurons and mitochondria of rat brains mediated by Aβ1-42, and these protective effects might be associated with the regulation of OGG1, Bad, Bcl-xl, ATPB and PDH.
authors.affiliation: Department of Nutrition & Food Hygiene, School of Public Health and Family Medicine, Capital Medical University, Beijing, China.
authors.forename: Jin-Fang
authors.initials: JF
authors.lastname: Feng
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: He
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Lin-Hong
authors.initials: LH
authors.lastname: Yuan
authors.forename: Huan-Ling
authors.initials: HL
authors.lastname: Yu
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Ma
authors.forename: Yue
authors.initials: Y
authors.lastname: Yang
authors.forename: Yuan-Di
authors.initials: YD
authors.lastname: Xi
authors.forename: Juan
authors.initials: J
authors.lastname: Ding
authors.forename: Yi-Xiu
authors.initials: YX
authors.lastname: Xiao
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D045647:DNA Glycosylases / Q000235:genetics / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D015536:Down-Regulation; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D010446:Peptide Fragments / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max / Q000737:chemistry*; D015854:Up-Regulation; D051025:bcl-Associated Death Protein / Q000235:genetics / Q000378:metabolism; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; C494821:Bad protein, rat; C494813:Bcl2l1 protein, rat; D007529:Isoflavones; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D012333:RNA, Messenger; C075222:amyloid beta-protein (1-42); D051025:bcl-Associated Death Protein; D051020:bcl-X Protein; D000080242:8-Hydroxy-2'-Deoxyguanosine; D045647:DNA Glycosylases; C483741:OGG1 protein, rat; D003849:Deoxyguanosine