title: Reverse genetics of influenza viruses.
abstract: The ability to modify influenza viruses at will has revolutionized influenza research. Reverse genetics has been used to generate mutant or reassortant influenza viruses to assess their replication, virulence, pathogenicity, host range, and transmissibility. Moreover, this technology is now being used to generate approved influenza virus vaccines and develop novel vaccines to combat seasonal and (future) pandemic influenza viruses. Several variations of the original system have been established, all of which are considerably robust and efficient.
authors.affiliation: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Neumann
authors.forename: Makoto
authors.initials: M
authors.lastname: Ozawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
mesh_terms: D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D058507:Host Specificity; D006801:Humans; D007251:Influenza, Human / Q000635:transmission / Q000821:virology; D009698:Nucleoproteins / Q000235:genetics*; D009975:Orthomyxoviridae / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics*; D059386:Reverse Genetics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: D017931:DNA Primers; D009698:Nucleoproteins; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases