title: Complete genome organization of American hop latent virus and its relationship to carlaviruses.
abstract: The complete genomic sequence of American hop latent virus (AHLV; genus Carlavirus) was determined. The genome consists of 8,601 nucleotides plus a 3'-polyadenylate tail. The genome encompasses six potential open reading frames (ORF) in the positive sense, and their organization is typical of other carlaviruses. Analysis of the coat protein coding sequence at both the nucleic acid level and the amino acid level indicates that AHLV is only remotely related to the other carlaviruses known to infect common hop. Polyclonal antibodies were produced against the bacterially expressed coat protein of AHLV. These antibodies differentiated between AHLV and other carlaviruses of hop.
authors.affiliation: Department of Plant Pathology, Washington State University, IAREC, 24106 North Bunn Road, Prosser, WA 99350, USA. keastwell@wsu.edu
authors.forename: Kenneth C
authors.initials: KC
authors.lastname: Eastwell
authors.forename: Keri L
authors.initials: KL
authors.lastname: Druffel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017788:Carlavirus / Q000145:classification / Q000235:genetics*; D027465:Chenopodium quinoa / Q000821:virology; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D016679:Genome, Viral; D027582:Humulus / Q000821:virology; D016013:Likelihood Functions; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D011817:Rabbits
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D012367:RNA, Viral