title: Human serum albumin-coated lipid nanoparticles for delivery of siRNA to breast cancer.
abstract: Human serum albumin (HSA)-coated lipid nanoparticles (HSA-LNPs) loaded with phrGFP-targeted siRNA (HSA-LNPs-siRNA) were prepared and evaluated for gene downregulation effect in phrGFP-transfected breast cancer cells and the corresponding xenograft tumor model. HSA-LNPs-siRNA were successfully prepared with a particle size of 79.5±5.5 nm. In phrGFP-transfected MCF-7 cells, HSA-LNPs-siRNA significantly decreased cell fluorescence even in the presence of fetal bovine serum (FBS). Moreover, cell fluorescence and phrGFP mRNA expression were significantly downregulated by HSA-LNPs-siRNA in phrGFP-transfected MCF-7, MDA-MB-231, and SK-BR-3 cells in comparison with control or HSA-LNPs-siRNA (scrambled). In phrGFP-transfected MCF-7 xenograft tumor model, tumor fluorescence was significantly decreased after three IV administrations of HSA-LNPs-siRNA at a dose of 3 mg/kg in comparison with siRNA alone. HSA-LNPs-siRNA demonstrated a superior pharmacokinetic profile in comparison with siRNA at a dose of 1mg/kg. These results show that the novel nonviral carrier, HSA-LNPs, may be used for the delivery of siRNA to breast cancer cells.
  
  
  BACKGROUND
  Targeted delivery of siRNA to cancer cells may be a viable anti-cancer strategy with low toxicity. In this study the novel nonviral carrier, human serum albumin-coated lipid nanoparticles (HSA-LNP) were demonstrated as an efficient delivery agent of siRNA to breast cancer cells.
authors.affiliation: Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Longzhu
authors.initials: L
authors.lastname: Piao
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Lesheng
authors.initials: L
authors.lastname: Teng
authors.forename: Bryant C
authors.initials: BC
authors.lastname: Yung
authors.forename: Yasuro
authors.initials: Y
authors.lastname: Sugimoto
authors.forename: Robert W
authors.initials: RW
authors.lastname: Brueggemeier
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D061986:MCF-7 Cells; D053758:Nanoparticles; D034741:RNA, Small Interfering / Q000235:genetics*; D012709:Serum Albumin / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D034741:RNA, Small Interfering; D012709:Serum Albumin