title: Effects of antifungal agents alone and in combination against Candida glabrata strains susceptible or resistant to fluconazole.
abstract: The rise of Candida spp. resistant to classic triazole antifungal agents has led to a search for new therapeutic options. Here, we evaluated combinations of antifungals in a checkerboard assay against two groups of Candida glabrata strains: one containing fluconazole-susceptible clinical isolates (FS) and another containing fluconazole-resistant laboratory derivative (FR). The most synergistic combination observed was amphotericin B + flucytosine (synergistic for 61.77 % of FS strains and 76.47 % of FR strains). The most antagonistic combination observed was ketoconazole + flucytosine (FS 61.77 % and FR 55.88 %). Surprisingly, most combinations evidenced indifferent interactions, and the best synergism appeared when amphotericin B and flucytosine were combined against both groups of isolates.
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Izabel Almeida
authors.initials: IA
authors.lastname: Alves
authors.forename: Laíssa Arévalo
authors.initials: LA
authors.lastname: Bandeira
authors.forename: Débora Alves Nunes
authors.initials: DA
authors.lastname: Mario
authors.forename: Laura Bedin
authors.initials: LB
authors.lastname: Denardi
authors.forename: Louise Vignoles
authors.initials: LV
authors.lastname: Neves
authors.forename: Janio Morais
authors.initials: JM
authors.lastname: Santurio
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D041221:Candida glabrata / Q000187:drug effects*; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology; D004347:Drug Interactions; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole