title: heterogeneity of dna ploidy in endometrial carcinoma: comparison of different tissue samples obtained during diagnosis and treatment.
abstract: OBJECTIVE
  Comparison of DNA ploidy status of different tumour tissue samples (fresh/frozen vs. paraffin-embedded; curettage vs. hysterectomy samples) obtained during diagnosis and treatment of patients with endometrial carcinoma.
  
  
  METHODS
  DNA ploidy status and conventional prognostic parameters were recorded for 74 patients with endometrial carcinoma prospectively.
  
  
  RESULTS
  In 59 (79.7%) patients the DNA status was described as diploid in all analyzed tissue samples. The remaining 15 (20.3%) cases were described as DNA aneuploid in at least one of the corresponding tissue samples. The concordance between DNA ploidy status in fresh vs. paraffin-embedded hysterectomy samples as well as curettage vs. hysterectomy paraffin-embedded samples was high (kappa coefficient κ=0.6348, 95% confidence interval CI=0.3673-0.9023, and p=0.6408, 95% CI=0.3977-0.8838), however, the methods are not interchangeable.
  
  
  CONCLUSIONS
  The DNA ploidy discordance observed in our study group seems to document intratumoral heterogeneity that should be expected when applying DNA ploidy status in the clinical management of endometrial carcinoma.
authors.affiliation: Department of Gynaecology and Obstetrics, Third Faculty of Medicine of Charles University and The Kralovske Vinohrady University Hospital, Ruska 87, 100 34 Prague, Czech Republic. pavla.libalova@lf3.cuni.cz
authors.forename: Pavla
authors.initials: P
authors.lastname: Libalova
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Vernerova
authors.forename: Lucie
authors.initials: L
authors.lastname: Hubickova-Heringova
authors.forename: Daniela
authors.initials: D
authors.lastname: Pinterova
authors.forename: Katarina
authors.initials: K
authors.lastname: Kolostova
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bobek
authors.forename: Karel
authors.initials: K
authors.lastname: Tikovsky
authors.forename: Daniel
authors.initials: D
authors.lastname: Housa
authors.forename: Martina
authors.initials: M
authors.lastname: Kubecova
authors.forename: Ladislav
authors.initials: L
authors.lastname: Pecen
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Svoboda
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D001706:Biopsy; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D004247:DNA / Q000235:genetics; D004171:Diploidy; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D004717:Endometrium / Q000473:pathology; D005260:Female; D006801:Humans; D007044:Hysterectomy; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D013048:Specimen Handling
chemical_list: D004247:DNA