title: Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection.
abstract: Human adipose-derived stem cells (huADSC) were generated from fat tissue of a 65-year-old male donor. Flow cytometry and reverse transcription polymerase chain reaction (RT-PCR) analyses indicated that the huADSC express neural cell proteins (MAP2, GFAP, nestin and β-III tubulin), neurotrophic growth factors (BDNF and GDNF), and the chemotactic factor CXCR4 and its corresponding ligand CXCL12. In addition, huADSC expressed the characteristic mesenchymal stem cell (MSC) markers CD29, CD44, CD73, CD90, CD105 and HLA class I. The huADSC were employed, via a right femoral vein injection, to treat rats inflicted with experimental intracerebral hemorrhage (ICH). Behavioral measurement on the experimental animals, seven days after the huADSC therapy, showed a significant functional improvement in the rats with stem cell therapy in comparison with rats of the control group without the stem cell therapy. The injected huADSC were detectable in the brains of the huADSC treated rats as determined by histochemistry analysis, suggesting a role of the infused huADSC in facilitating functional recovery of the experimental animals with ICH induced stroke.
authors.affiliation: Buddhist Tzu Chi Stem Cells Center, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan. edward@tzuchi.com.tw
authors.forename: Kuo-Liang
authors.initials: KL
authors.lastname: Yang
authors.forename: Jiunn-Tat
authors.initials: JT
authors.lastname: Lee
authors.forename: Cheng-Yoong
authors.initials: CY
authors.lastname: Pang
authors.forename: Ting-Yi
authors.initials: TY
authors.lastname: Lee
authors.forename: Shee-Ping
authors.initials: SP
authors.lastname: Chen
authors.forename: Hock-Kean
authors.initials: HK
authors.lastname: Liew
authors.forename: Shin-Yuan
authors.initials: SY
authors.lastname: Chen
authors.forename: Tzu-Yung
authors.initials: TY
authors.lastname: Chen
authors.forename: Py-Yu
authors.initials: PY
authors.lastname: Lin
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D000368:Aged; D000818:Animals; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000628:therapy*; D017364:Collagenases / Q000008:administration & dosage; D005268:Femoral Vein; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D003012:Microbial Collagenase; D051381:Rats; D017207:Rats, Sprague-Dawley; D044968:Regenerative Medicine; D020521:Stroke / Q000628:therapy
chemical_list: D017364:Collagenases; C533954:collagenase VII-S, Clostridium histolyticum; D003012:Microbial Collagenase