title: Flexibility of a eukaryotic lipidome--insights from yeast lipidomics.
abstract: Mass spectrometry-based shotgun lipidomics has enabled the quantitative and comprehensive assessment of cellular lipid compositions. The yeast Saccharomyces cerevisiae has proven to be a particularly valuable experimental system for studying lipid-related cellular processes. Here, by applying our shotgun lipidomics platform, we investigated the influence of a variety of commonly used growth conditions on the yeast lipidome, including glycerophospholipids, triglycerides, ergosterol as well as complex sphingolipids. This extensive dataset allowed for a quantitative description of the intrinsic flexibility of a eukaryotic lipidome, thereby providing new insights into the adjustments of lipid biosynthetic pathways. In addition, we established a baseline for future lipidomic experiments in yeast. Finally, flexibility of lipidomic features is proposed as a new parameter for the description of the physiological state of an organism.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Klose
authors.forename: Michal A
authors.initials: MA
authors.lastname: Surma
authors.forename: Mathias J
authors.initials: MJ
authors.lastname: Gerl
authors.forename: Felix
authors.initials: F
authors.lastname: Meyenhofer
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
authors.forename: Kai
authors.initials: K
authors.lastname: Simons
mesh_terms: D002244:Carbon / Q000378:metabolism; D016000:Cluster Analysis; D050356:Lipid Metabolism; D008055:Lipids / Q000145:classification; D013058:Mass Spectrometry; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
chemical_list: D008055:Lipids; D002244:Carbon