title: RAB-27 and its effector RBF-1 regulate the tethering and docking steps of DCV exocytosis in C. elegans.
abstract: The molecular mechanisms by which dense core vesicles (DCVs) translocate, tether, dock and prime are poorly understood. In this study, Caenorhabditis elegans was used as a model organism to study the function of Rab proteins and their effectors in DCV exocytosis. RAB-27/AEX-6, but not RAB-3, was found to be required for peptide release from neurons. By analyzing the movement of DCVs approaching the plasma membrane using total internal reflection fluorescence microscopy, we demonstrated that RAB-27/AEX-6 is involved in the tethering of DCVs and that its effector rabphilin/RBF-1 is required for the initial tethering and subsequent stabilization by docking.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: WanJuan
authors.initials: W
authors.lastname: Feng
authors.forename: Tao
authors.initials: T
authors.lastname: Liang
authors.forename: JunWei
authors.initials: J
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: YongDeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: ZhengXing
authors.initials: Z
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology; D005089:Exocytosis; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D022142:Secretory Vesicles / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D033921:Vesicular Transport Proteins / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D000075745:rab27 GTP-Binding Proteins; D000097624:Rabphilin-3A
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D029742:Caenorhabditis elegans Proteins; D049452:Green Fluorescent Proteins; D009419:Nerve Tissue Proteins; D010455:Peptides; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins; D000075745:rab27 GTP-Binding Proteins; D000097624:Rabphilin-3A; C443319:rbf-1 protein, C elegans; C534877:aex-6 protein, C elegans