title: Enhancement of long-term angiogenic efficacy of adipose stem cells by delivery of FGF2.
abstract: Stem cell transplantation can induce neovascularization. Regenerated blood vessels should remain stable for a long-term period in order to function as new blood vessels in ischemic tissues. Here we show that local delivery of FGF2 enhances the long-term (12weeks) angiogenic efficacy of human adipose-derived stem cells (hADSCs) implanted into mouse ischemic hindlimbs. Following transplantation of hADSCs into ischemic hindlimbs of mice, hADSC viability was significantly higher in the hADSC+FGF2 group at 4 and 12weeks post-transplantation than in the hADSC only group. Furthermore, hADSCs produced higher levels of angiogenic growth factors (i.e., fibroblast growth factor 2, vascular endothelial growth factor, hepatocyte growth factor, and platelet-derived growth factor) at both time points. As a result, the density of arterioles in the ischemic hindlimb muscle was significantly higher in the hADSC+FGF2 group than in either hADSC or FGF2 only group at both time points. The number of arterioles with larger diameters was significantly greater in the hADSC+FGF2 group than in the other groups at 12weeks, and increased in the hADSC+FGF2 group as the time period increased from 4weeks to 12weeks post-transplantation. This suggests that FGF2 delivery to hADSC transplantation sites enhances long-term angiogenic efficacy of hADSCs transplanted into ischemic tissues.
authors.affiliation: Department of Bioengineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul, 133-791, Republic of Korea.
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Lee
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Bhang
authors.forename: Hee Seok
authors.initials: HS
authors.lastname: Yang
authors.forename: Wan-Guen
authors.initials: WG
authors.lastname: La
authors.forename: Hee Hun
authors.initials: HH
authors.lastname: Yoon
authors.forename: Jung-Youn
authors.initials: JY
authors.lastname: Shin
authors.forename: Jun Yeup
authors.initials: JY
authors.lastname: Seong
authors.forename: Heungsoo
authors.initials: H
authors.lastname: Shin
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001160:Arterioles / Q000187:drug effects / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D005346:Fibroblast Growth Factors / Q000008:administration & dosage* / Q000378:metabolism; D006614:Hindlimb / Q000473:pathology; D007511:Ischemia / Q000628:therapy; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D009132:Muscles / Q000098:blood supply / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000502:physiology*; D014183:Transplantation, Heterologous
chemical_list: D015415:Biomarkers; C416003:FGF20 protein, human; D005346:Fibroblast Growth Factors