title: Microwave denture disinfection versus nystatin in treating patients with well-controlled type 2 diabetes and denture stomatitis: a randomized clinical trial.
abstract: OBJECTIVE
  The aim of this randomized clinical trial was to compare the effectiveness of microwave denture disinfection and nystatin in the treatment of well-controlled type 2 diabetic patients with denture stomatitis in terms of microbiologic and clinical outcomes.
  
  
  METHODS
  Diabetic patients wearing maxillary complete dentures with denture stomatitis (n = 40) were divided into two groups: NYS (patients treated with topical nystatin 4 times/day for 14 days) and MW (patients who had their dentures microwaved [650 W for 3 minutes] 3 times/week for 14 days). Mycologic samples were taken from the palates and dentures of the patients for quantification and identification of Candida, and standardized photographs of the palates were taken for clinical analysis. Evaluations were repeated at baseline, the end of treatment (day 14), and throughout follow-up (days 30, 60, and 90). Microbiologic data were evaluated by analysis of variance using a random effects statistical model, Tukey post hoc test, and chi-square test (α = .05). Clinical results were analyzed using Mann-Whitney and Fisher exact tests (α = .05).
  
  
  RESULTS
  Both treatments were considered successful in reducing the clinical signs of denture stomatitis and significantly reduced the values of colony-forming units/mL from the palates and dentures at days 14 and 30. In addition, 40% of treated patients were cured by the end of treatment. No significant differences in the microbiologic and clinical outcomes were revealed between the two groups (P > .05). C albicans was the most predominant species isolated (P < .01), followed by C tropicalis and C glabrata.
  
  
  CONCLUSIONS
  Denture microwave disinfection was as effective as nystatin for the treatment of diabetic patients with denture stomatitis.
authors.affiliation: Univ Estadual Paulista, Sao Paulo, Brazil.
authors.forename: Paula Volpato
authors.initials: PV
authors.lastname: Sanita
authors.forename: Ana Lucia
authors.initials: AL
authors.lastname: Machado
authors.forename: Ana Claudia
authors.initials: AC
authors.lastname: Pavarina
authors.forename: Elaine Maria Sgavioli
authors.initials: EM
authors.lastname: Massucato
authors.forename: Arnaldo Lopes
authors.initials: AL
authors.lastname: Colombo
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Vergani
mesh_terms: D000287:Administration, Topical; D000368:Aged; D000704:Analysis of Variance; D000935:Antifungal Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002175:Candida / Q000302:isolation & purification; D016009:Chi-Square Distribution; D015169:Colony Count, Microbial; D003824:Denture, Complete / Q000009:adverse effects / Q000382:microbiology*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications; D004203:Disinfection / Q000379:methods*; D005260:Female; D006801:Humans; D016013:Likelihood Functions; D008297:Male; D008872:Microwaves; D008875:Middle Aged; D009761:Nystatin / Q000008:administration & dosage / Q000627:therapeutic use*; D021362:Palate, Hard / Q000382:microbiology; D016037:Single-Blind Method; D013282:Stomatitis, Denture / Q000150:complications / Q000209:etiology / Q000382:microbiology / Q000628:therapy*
chemical_list: D000935:Antifungal Agents; D009761:Nystatin