title: Magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG), potential application to early pregnancy diagnosis.
abstract: The objective of this study was to develop a magnetic particle-linked monoclonal antibody to hCG β for immunosorbent assay of human chorionic gonadotropin (hCG) with improved detection sensitivity. Monoclonal antibody against hCG β was found to be optimally cross-linked to the superparamagnetic particles (SPIO) using EDC and NHS as cross-linking reagents. This superparamagnetic particle-linked monoclonal antibody was able to concentrate hCG from a tested solution for further ELISA assay using horse radish peroxidase-labeled monoclonal antibody against hCG β. This hybrid technique had greatly decreased the detection limit to 0.1 mIU/mL, making an early detection of pregnancy possible. With an improved sensitivity and simple operation, the magnetic particle-linked anti hCG β antibody for immunoassay of human chorionic gonadotropin (hCG) has a great potential to supersede the traditional ELISA for pregnancy diagnosis.
authors.affiliation: Department of Food Science, National Pingtung University of Science and Technology, 1 Hseuh Fu Road, Neipei, Pingtung 91201 Taiwan.
authors.forename: Hsiao-Ting
authors.initials: HT
authors.lastname: Kuo
authors.forename: Jay Z
authors.initials: JZ
authors.lastname: Yeh
authors.forename: Chi-Ming
authors.initials: CM
authors.lastname: Jiang
authors.forename: Ming-Chang
authors.initials: MC
authors.lastname: Wu
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000032:analysis / Q000276:immunology*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D011247:Pregnancy; D011258:Pregnancy Tests / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D018997:Chorionic Gonadotropin, beta Subunit, Human; D058185:Magnetite Nanoparticles