title: Hybridization-triggered isothermal signal amplification coupled with MutS for label-free and sensitive fluorescent assay of SNPs.
abstract: Combining the specific recognization of MutS protein for mismatched DNA sequences with the target-driven molecular switch that acts as both the template and primer of the polymerization reaction, a new label-free and sensitive fluorescent assay strategy for specific single-stranded DNA sequences or SNPs is proposed.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and College of Materials and Engineering, Hunan University, Changsha, 410082, PR China.
authors.forename: Anli
authors.initials: A
authors.lastname: Jiao
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.forename: Yaping
authors.initials: Y
authors.lastname: Hu
authors.forename: Guizhi
authors.initials: G
authors.lastname: Zhu
authors.forename: Jishan
authors.initials: J
authors.lastname: Li
authors.forename: Huimin
authors.initials: H
authors.lastname: Li
authors.forename: Ronghua
authors.initials: R
authors.lastname: Yang
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D052160:Benzothiazoles; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D003959:Diamines; D005456:Fluorescent Dyes / Q000032:analysis; D051719:MutS DNA Mismatch-Binding Protein / Q000378:metabolism*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009930:Organic Chemicals / Q000032:analysis; D020641:Polymorphism, Single Nucleotide; D011804:Quinolines; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D004247:DNA; D051719:MutS DNA Mismatch-Binding Protein