title: GFP-expressing influenza A virus for evaluation of the efficacy of antiviral agents.
abstract: To address its value as a screening tool in the development of antiviral drugs, a recombinant influenza virus expressing green fluorescent protein (rPR8-GFP virus) was investigated in vitro and in vivo. The inhibition of viral growth by a neuraminidase inhibitor in the cells or lower respiratory tracts of mice could be visualized by the level of fluorescence. In addition, the rPR8-GFP virus exhibited high pathogenicity in mice. Taken together, these results suggest that the rPR8-GFP virus can be a useful tool for the rapid identification of antiviral drugs active against influenza viruses.
authors.affiliation: Department of Microbiology, Hallym University, Chuncheon, 200-702, Republic of Korea.
authors.forename: Jin Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Sehee
authors.initials: S
authors.lastname: Park
authors.forename: Ilseob
authors.initials: I
authors.lastname: Lee
authors.forename: Sangmoo
authors.initials: S
authors.lastname: Lee
authors.forename: Saem
authors.initials: S
authors.lastname: Shin
authors.forename: Yongkwan
authors.initials: Y
authors.lastname: Won
authors.forename: Min-Woong
authors.initials: MW
authors.lastname: Hwang
authors.forename: Joon-Yong
authors.initials: JY
authors.lastname: Bae
authors.forename: Jun
authors.initials: J
authors.lastname: Heo
authors.forename: Hye-Eun
authors.initials: HE
authors.lastname: Hyun
authors.forename: Hyejin
authors.initials: H
authors.lastname: Jun
authors.forename: Soon Sung
authors.initials: SS
authors.lastname: Lim
authors.forename: Man-Seong
authors.initials: MS
authors.lastname: Park
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D009980:Influenza A virus / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D007251:Influenza, Human / Q000188:drug therapy / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014774:Virulence
chemical_list: D000998:Antiviral Agents; D004791:Enzyme Inhibitors; D049452:Green Fluorescent Proteins