title: NCPP treatment alleviates ConA-induced hepatitis via reducing CD4+T activation and NO production.
abstract: Corynebacterium parvum (CP), a kind of immunomodulator, has been well documented in many diseases. Non-cell C. parvum product (NCPP) is a newly-found nano-preparation. To investigate the effect of NCPP on Con A-induced murine severe hepatitis, we pretreated mice with NCPP intraperitoneally. After 12 h, ConA (25 μg/g body wt) was injected intravenously to provoke severe hepatitis and the degree of liver injury was evaluated by serum transaminase analysis and heptatic tissue pathology. Results have shown that levels of serum transaminase and degree of liver injury in ConA/NCPP groups had significantly declined than those in ConA/PBS groups. Notably, results of flow cytometry have demonstrated that activation of CD4+T cells in ConA/NCPP groups has been down-regulated, compared with ConA/PBS groups. Further, levels of serum and KC-related nitric oxide (NO) was displayed significantly lower in ConA/NCPP groups than those in ConA/PBS groups. The results indicate that NCPP may alleviate ConA-induced hepatitis by reducing CD4+T activation and NO production.
authors.affiliation: Department of Immunology, Shandong University School of Medicine, Jinan, China.
authors.forename: Chenghu
authors.initials: C
authors.lastname: Liu
authors.forename: Shangxian
authors.initials: S
authors.lastname: Gao
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Qu
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.forename: Faliang
authors.initials: F
authors.lastname: Zhu
authors.forename: Chun
authors.initials: C
authors.lastname: Guo
authors.forename: Li
authors.initials: L
authors.lastname: Hou
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Shi
authors.forename: Lining
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002457:Cell Extracts / Q000737:chemistry / Q000494:pharmacology*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D003208:Concanavalin A / Q000009:adverse effects* / Q000494:pharmacology; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000009:adverse effects* / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000276:immunology; D011425:Propionibacterium acnes / Q000737:chemistry*
chemical_list: D002457:Cell Extracts; D008934:Mitogens; D003208:Concanavalin A; D009569:Nitric Oxide