title: Development of antibiotic and debriding enzyme-loaded PLGA microspheres entrapped in PVA-gelatin hydrogel for complete wound management.
abstract: A biocompatible moist system was developed for effective and complete wound healing. Optimized PLGA microspheres of gentamicin (GM) and serratiopeptidase (STP) were incorporated into PVA-gelatin slurry and casted into films to prepare multiphase hydrogel. The prepared system was characterized by in vitro and in vivo studies. Results revealed the uniform dispersion of microspheres in three-dimensional matrix of the hydrogel. The in vitro release data showed a typical biphasic release pattern. All parameters such as wound contraction, tensile strength, histopathological and biochemical parameters were observed significant (p < 0.05) in comparison to the control group. Results suggested an accelerated re-epithelialization with minimum disturbance of wound bed.
authors.affiliation: University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, India. deependraiop@gmail.com
authors.forename: Deependra
authors.initials: D
authors.lastname: Singh
authors.forename: Manju Rawat
authors.initials: MR
authors.lastname: Singh
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000378:metabolism; D003646:Debridement / Q000379:methods*; D004338:Drug Combinations; D005780:Gelatin / Q000378:metabolism; D005839:Gentamicins / Q000008:administration & dosage* / Q000378:metabolism; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D020100:Hydrogels / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D051379:Mice; D008863:Microspheres; D009119:Muscle Contraction; D009924:Organ Culture Techniques; D010447:Peptide Hydrolases / Q000008:administration & dosage* / Q000378:metabolism; D011100:Polyglycolic Acid / Q000378:metabolism; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011142:Polyvinyl Alcohol / Q000378:metabolism; D062326:Re-Epithelialization; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D004338:Drug Combinations; D005839:Gentamicins; D020100:Hydrogels; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005780:Gelatin; D011142:Polyvinyl Alcohol; D010447:Peptide Hydrolases; C022640:serratiopeptidase