title: Genotype identification of Enterocytozoon bieneusi isolates from stool samples of HIV-infected Tunisian patients.
abstract: The microsporidian species Enterocytozoon bieneusi is a major cause of chronic diarrhea and malabsorption in patients with AIDS. Genotyping was performed on seven E. bieneusi strains for the first time in Tunisia. All the strains were isolated from stool samples of humans with immunodeficiency virus (HIV) infection. Analysis of the ribosomal RNA gene internal transcribed spacer (rDNA ITS) allowed the identification of three distinct genotypes previously described in other studies. Genotypes D and B were characterized in four and two respectively. The Peruvian genotype (Peru 8) was detected in the last isolate. These results indicate a genetic diversity in E. bieneusi strains from HIV Tunisian patients and suggest the coexistence of both zoonotic and anthroponotic route of transmission.
authors.affiliation: Laboratoire de recherche Parasitoses médicales, biotechnologie et bio-molécules LR 11-IPT-06 & Laboratoire de parasitologie, Institut Pasteur de Tunis, Tunisie.
authors.forename: N
authors.initials: N
authors.lastname: Chabchoub
authors.forename: R
authors.initials: R
authors.lastname: Abdelmalek
authors.forename: J
authors.initials: J
authors.lastname: Breton
authors.forename: F
authors.initials: F
authors.lastname: Kanoun
authors.forename: M
authors.initials: M
authors.lastname: Thellier
authors.forename: A
authors.initials: A
authors.lastname: Bouratbine
authors.forename: K
authors.initials: K
authors.lastname: Aoun
mesh_terms: D000328:Adult; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry; D021862:Enterocytozoon / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000150:complications* / Q000453:epidemiology; D006801:Humans; D016867:Immunocompromised Host; D007223:Infant; D008297:Male; D016881:Microsporidiosis / Q000453:epidemiology / Q000469:parasitology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014416:Tunisia / Q000453:epidemiology; D055815:Young Adult
chemical_list: D016054:DNA, Protozoan; D012335:RNA, Ribosomal