title: Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a potentially useful tool for the detection of antimicrobial resistance, especially that conferred by β-lactamases. Here we describe a modification of a previously reported MALDI-TOF MS meropenem hydrolysis assay. The modified method was validated on 108 carbapenemase-producing members of the Enterobacteriaceae, two NDM-1-producing Acinetobacter baumannii isolates, and 35 carbapenem-resistant enterobacteria producing no carbapenemase. The detection of carbapenemases by MALDI-TOF MS seems to be a powerful, quick, and cost-effective method for microbiological laboratories.
authors.affiliation: Department of Microbiology, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic. jaroslav.hrabak@lfp.cuni.cz
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Hrabák
authors.forename: Vendula
authors.initials: V
authors.lastname: Studentová
authors.forename: Radka
authors.initials: R
authors.lastname: Walková
authors.forename: Helena
authors.initials: H
authors.lastname: Zemlicková
authors.forename: Vladislav
authors.initials: V
authors.lastname: Jakubu
authors.forename: Eva
authors.initials: E
authors.lastname: Chudácková
authors.forename: Marek
authors.initials: M
authors.lastname: Gniadkowski
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Pfeifer
authors.forename: John D
authors.initials: JD
authors.lastname: Perry
authors.forename: Kathryn
authors.initials: K
authors.lastname: Wilkinson
authors.forename: Tamara
authors.initials: T
authors.lastname: Bergerová
mesh_terms: D040981:Acinetobacter baumannii / Q000201:enzymology*; D001426:Bacterial Proteins / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000201:enzymology*; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D006868:Hydrolysis; D000077731:Meropenem; D008826:Microbial Sensitivity Tests / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013845:Thienamycins / Q000378:metabolism*; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D013845:Thienamycins; D001618:beta-Lactamases; C063912:carbapenemase; D000077731:Meropenem