title: Enhancing follicular growth as a prerequisite for GnRH treatment of true anestrum in buffalo.
abstract: A total of 140 true anestrous buffalo were divided on the basis of receiving short-term (20 days) nutritional supplementation (N, n=80) or not (WN, n=60). The animals in N group were subdivided into NQ (n=40) where the quantity of the offered diet was increased by 20% and NF (n=40) where the offered diet was supplemented by 3% of dry protected fat. Buffaloes in either NQ or NF were equally allotted on the following treatment regimens: Insulin/GnRH (NQi or NFi, n=10 for each); rbST/GnRH (NQr or NFr, n=10 for each); GnRH alone treated (NQG or NFG, n=10 for each) and saline-treated control (NQc or NFc, n=10 for each). Insulin-treated subgroups (NQi or NFi) received s/c injection of insulin at a dose of 0.25 I.U./kg on Days 21, 22 and 23 while rbST-treated subgroups (NQr or NFr) received single IM injection of rbST (500 mg Sometribove) on Day 21. GnRH was injected at a dose of 0.020 mg buserelin (5 ml Receptal(®)) on Day 24 in all subgroups except NQCand NFC where Day 1 was the first day of the short-term nutritional improvement. Buffalo in the WN (n=60) were equally allotted on the same treatment regimens applied in the first group insulin/GnRH (WNi, n=15), rbST/GnRH (WNr, n=15); GnRH alone treated (WNG, n=15) and saline-treated control (WNC, n=15). Ultrasonic scanning of ovaries was conducted on Day 24 to measure largest follicle diameter (LFD). The results showed increases (P<0.05) in the LFD following nutritional supplementation with insulin or rbST. The recorded EIRs for GnRH pre-treated with nutritional improvement - metabolic hormones combinations (9/10 and 8/10 for NQi and NFi or 8/10 for NQr) were greater (P<0.05) than those pre-treated with either metabolic hormone alone (7/15 for WNi and/or WNr) or nutritional improvement alone (6/10 for NQG and/or NFG) and control as well. The greatest CR was recorded in the NQi group. It could be concluded that pre-GnRH nutritional improvement plus administration of insulin or rbST increases LFD in true anestrous buffalo having LFD<8.5 mm thereby increasing their fertility response to GnRH in terms of EIRs and CRs.
authors.affiliation: Theriogenology Department, Faculty of Veterinary Medicine, Kafrelesheikh University, Kafr El-Sheikh, Egypt. ramoun2004@yahoo.com
authors.forename: A A
authors.initials: AA
authors.lastname: Ramoun
authors.forename: B H
authors.initials: BH
authors.lastname: Serur
authors.forename: El-S M
authors.initials: el-SM
authors.lastname: Fattouh
authors.forename: S A
authors.initials: SA
authors.lastname: Darweish
authors.forename: H A
authors.initials: HA
authors.lastname: Abou El-Ghait
mesh_terms: D000780:Anestrus / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002020:Buffaloes / Q000097:blood / Q000502:physiology*; D016009:Chi-Square Distribution; D019587:Dietary Supplements; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage*; D013006:Growth Hormone / Q000008:administration & dosage*; D007328:Insulin / Q000008:administration & dosage* / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000254:growth & development / Q000502:physiology*; D049590:Postpartum Period; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011897:Random Allocation; D014463:Ultrasonography
chemical_list: D001786:Blood Glucose; D007328:Insulin; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone