title: ESTs library from embryonic stages reveals tubulin and reflectin diversity in Sepia officinalis (Mollusca — Cephalopoda).
abstract: New molecular resources regarding the so-called “non-standard models” in biology extend the present knowledge and are essential for molecular evolution and diversity studies (especially during the development) and evolutionary inferences about these zoological groups, or more practically for their fruitful management. Sepia officinalis, an economically important cephalopod species, is emerging as a new lophotrochozoan developmental model. We developed a large set of expressed sequence tags (ESTs) from embryonic stages of S. officinalis, yielding 19,780 non-redundant sequences (NRS). Around 75% of these sequences have no homologs in existing available databases. This set is the first developmental ESTs library in cephalopods. By exploring these NRS for tubulin, a generic protein family, and reflectin, a cephalopod specific protein family,we point out for both families a striking molecular diversity in S. officinalis.
authors.affiliation: Muséum National d'Histoire Naturelle (MNHN), Département Milieux et Peuplements Aquatiques (DMPA), UMR Biologie des ORganismes et Ecosystèmes Aquatiques (BOREA), MNHN, CNRS 7208, IRD 207, UPMC. Paris, France. bassaglia@mnhn.fr
authors.forename: Yann
authors.initials: Y
authors.lastname: Bassaglia
authors.forename: Thomas
authors.initials: T
authors.lastname: Bekel
authors.forename: Corinne
authors.initials: C
authors.lastname: Da Silva
authors.forename: Julie
authors.initials: J
authors.lastname: Poulain
authors.forename: Aude
authors.initials: A
authors.lastname: Andouche
authors.forename: Sandra
authors.initials: S
authors.lastname: Navet
authors.forename: Laure
authors.initials: L
authors.lastname: Bonnaud
mesh_terms: D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D004625:Embryo, Nonmammalian / Q000502:physiology; D020224:Expressed Sequence Tags; D005260:Female; D015723:Gene Library; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D010802:Phylogeny; D049833:Sepia / Q000196:embryology / Q000235:genetics*; D014404:Tubulin / Q000235:genetics*
chemical_list: D000199:Actins; D014404:Tubulin