title: Binding of dynein intermediate chain 2 to paxillin controls focal adhesion dynamics and migration.
abstract: In migrating NRK cells, aPKCs control the dynamics of turnover of paxillin-containing focal adhesions (FA) determining migration rate. Using a proteomic approach (two-dimensional fluorescence difference gel electrophoresis), dynein intermediate chain 2 (dynein IC2) was identified as a protein that is phosphorylated inducibly during cell migration in a PKC-regulated manner. By gene silencing and co-immunoprecipitation studies, we show that dynein IC2 regulates the speed of cell migration through its interaction with paxillin. This interaction is controlled by serine 84 phosphorylation, which lies on the aPKC pathway. The evidence presented thus links aPKC control of migration to the dynein control of FA turnover through paxillin.
authors.affiliation: Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, London WC2A 3PX, UK. carine.rosse@curie.fr
authors.forename: Carine
authors.initials: C
authors.lastname: Rosse
authors.forename: Katrina
authors.initials: K
authors.lastname: Boeckeler
authors.forename: Mark
authors.initials: M
authors.lastname: Linch
authors.forename: Simone
authors.initials: S
authors.lastname: Radtke
authors.forename: David
authors.initials: D
authors.lastname: Frith
authors.forename: Karin
authors.initials: K
authors.lastname: Barnouin
authors.forename: Ali Sayed
authors.initials: AS
authors.lastname: Morsi
authors.forename: Majid
authors.initials: M
authors.lastname: Hafezparast
authors.forename: Michael
authors.initials: M
authors.lastname: Howell
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Parker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D056445:Cytoplasmic Dyneins; D004398:Dyneins / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000201:enzymology; D008969:Molecular Sequence Data; D051419:Paxillin / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats
chemical_list: D051419:Paxillin; C424131:DYNC1LI1 protein, human; D056445:Cytoplasmic Dyneins; D004398:Dyneins