title: Identification and location of symbionts associated with potato psyllid (Bactericera cockerelli) lifestages.
abstract: The potato psyllid (Bactericera cockerelli, Sulc) is an invasive pest of solenaceous plants including potatoes (Solanum tuberosum L.)and tomatoes (Solanum lycopersicum L.). The insect transmits the phytopathogen Candidatus Liberibacter solanacearum, which has been identified as the causal agent of Zebra Chip in potatoes. The microbiome of the potato psyllid provides knowledge of the insect's bacterial makeup which enables researchers to develop targeted biological control strategies. In this study, the microbes associated with four B. cockerelli life stages were evaluated by 16S bTEFAP pyrosequencing. The sequences were compared with a 16S-rDNA database derived from NCBI's GenBank. Some bacteria identified are initial discoveries. Species of Wolbachia, Rhizobium, Gordonia, Mycobacterium, Xanthomonas and others were also detected and an assessment of the microbiome associated with B. cockerelli was established.
authors.affiliation: University of Texas, Tyler 3900 University Blvd., Tyler, TX 75701-6699, USA. daymon.hail@gmail.com
authors.forename: Daymon
authors.initials: D
authors.lastname: Hail
authors.forename: Scot E
authors.initials: SE
authors.lastname: Dowd
authors.forename: Blake
authors.initials: B
authors.lastname: Bextine
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006430:Hemiptera / Q000382:microbiology*; D054892:Metagenome; D009758:Nymph / Q000382:microbiology; D010063:Ovum / Q000382:microbiology; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S