title: Usefulness of 16S rDNA sequencing for the diagnosis of infective endocarditis caused by Corynebacterium diphtheriae.
abstract: We report a rare case of infective endocarditis caused by Corynebacterium diphtheriae in an 8-year-old boy, 2 years after a right ventricular outflow tract reconstruction with a bovine Contegra valved conduit. The patient recovered well after an RV-PA conduit enblock explantation and replacement with an aortic homograft with antibiotic treatment. All bacteriological cultures of excised tissue and blood were negative. The aetiological agent was identified as C. diphtheriae subsp. gravis by 16s rDNA sequencing.
authors.affiliation: Department of Microbiology, Frontier Lifeline Hospital, Mogappair, Chennai, India.
authors.forename: Padmaja
authors.initials: P
authors.lastname: Pathipati
authors.affiliation: Department of Microbiology, University of Madras, Dr ALM Post Graduate Institute of Basic Medical Sciences, Taramani, Chennai, India.
authors.forename: Thangam
authors.initials: T
authors.lastname: Menon
authors.affiliation: Department of Microbiology, University of Madras, Dr ALM Post Graduate Institute of Basic Medical Sciences, Taramani, Chennai, India.
authors.forename: Naveen
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Department of Microbiology, Frontier Lifeline Hospital, Mogappair, Chennai, India.
authors.forename: Thara
authors.initials: T
authors.lastname: Francis
authors.affiliation: Department of Paediatric Cardiology, Frontier Lifeline Hospital, Mogappair, Chennai, India.
authors.forename: Prem
authors.initials: P
authors.lastname: Sekar
authors.affiliation: Department of Cardiothoracic Surgery, Frontier Lifeline Hospital, Mogappair, Chennai, India.
authors.forename: Kotturathu Mammen
authors.initials: KM
authors.lastname: Cherian
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001431:Bacteriological Techniques / Q000379:methods*; D002648:Child; D003354:Corynebacterium Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology* / Q000601:surgery; D003353:Corynebacterium diphtheriae / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004696:Endocarditis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology* / Q000601:surgery; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014694:Ventricular Outflow Obstruction / Q000601:surgery
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S