title: Proteomic characterization of the human FTSJ3 preribosomal complexes.
abstract: In eukaryotes, ribosome biogenesis involves excision of transcribed spacer sequences from the preribosomal RNA, base and ribose covalent modification at specific sites, assembly of ribosomal proteins, and transport of subunits from the nucleolus to the cytoplasm where mature ribosomes engage in mRNA translation. The biochemical reactions throughout ribosome synthesis are mediated by factors that associate transiently to the preribosomal complexes. In this work, we describe the complexes containing the human protein FTSJ3. This protein functions in association with NIP7 in ribosome synthesis and contains a putative RNA-methyl-transferase domain (FtsJ) in the N-terminal region and two uncharacterized domains in the central (DUF3381) and C-terminal (Spb1_C) regions. FLAG-tagged FTSJ3 coimmunoprecipitates both RPS and RPL proteins, ribosome synthesis factors, and proteins whose function in ribosome synthesis has not been demonstrated yet. A similar set of proteins coimmunoprecipitates with the Spb1_C domain, suggesting that FTSJ3 interaction with the preribosome complexes is mediated by the Spb1_C domain. Approximately 50% of the components of FTSJ3 complexes are shared by complexes described for RPS19, Par14, nucleolin, and NOP56. A significant number of factors are also found in complexes described for nucleophosmin, SBDS, ISG20L2, and NIP7. These findings provide information on the dynamics of preribosome complexes in human cells.
authors.affiliation: Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais , Rua Giuseppe Maximo Scolfaro 10000, P.O. Box 6192, CEP 13083-970, Campinas SP, Brazil.
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Simabuco
authors.forename: Luis G
authors.initials: LG
authors.lastname: Morello
authors.forename: Annelize Zambon Barbosa
authors.initials: AZ
authors.lastname: Aragão
authors.forename: Adriana Franco
authors.initials: AF
authors.lastname: Paes Leme
authors.forename: Nilson I T
authors.initials: NI
authors.lastname: Zanchin
mesh_terms: D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D008780:Methyltransferases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009687:Nuclear Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D040901:Proteomics; D012269:Ribosomal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: C499553:NIP7 protein, human; D009687:Nuclear Proteins; D012269:Ribosomal Proteins; C040343:ribosomal protein S19; C574541:FTSJ3 protein, human; D008780:Methyltransferases