title: In vivo metabolism study of vaccarin in rat using HPLC-LTQ-MSn.
abstract: In order to illustrate the main biotransformation pathways of vaccarin in vivo, metabolites of vaccarin in rats were identified using a specific and sensitive high-performance liquid chromatography-electrospray ionization linear ion trap mass spectrometry (LTQ XL™) method. The rats were administered a single dose (200 mg/kg) of vaccarin by oral gavage. By comparing their changes in molecular masses (ΔM), retention times and spectral patterns with those of the parent drug, the parent compound and six metabolites were found in rat urine after oral administration of vaccarin. The parent compound and five metabolites were detected in rat plasma. In heart, liver and kidney samples, respectively, one, four and three metabolites were identified, in addition to the parent compound. Three metabolites, but no trace of parent drug, were found in the rat feces. This is the first systematic metabolism study of vaccarin in vivo. The biotransformation pathways of vaccarin involved methylation, hydroxylation, glycosylation and deglycosylation.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Qi
authors.forename: Mingsong
authors.initials: M
authors.lastname: Fan
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Li
authors.forename: Mingcang
authors.initials: M
authors.lastname: Chen
authors.forename: Zhaolin
authors.initials: Z
authors.lastname: Sun
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Chenggang
authors.initials: C
authors.lastname: Huang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry; D005419:Flavonoids / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics / Q000652:urine; D006027:Glycosides / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics / Q000652:urine; D006031:Glycosylation; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D008745:Methylation; D009206:Myocardium / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D005419:Flavonoids; D006027:Glycosides