title: Immobilisation of quantum dots by bio-orthogonal PCR amplification and labelling for direct gene detection and quantitation.
abstract: A sensitive and versatile detection scheme based on quantum dot immobilisation on a solid support through bio-orthogonal PCR amplification and labelling has been developed for detection and quantification of gene targets in complex DNA mixtures.
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543.
authors.forename: Quan
authors.initials: Q
authors.lastname: Tian
authors.forename: Winnie
authors.initials: W
authors.lastname: Wong
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Yinthai
authors.initials: Y
authors.lastname: Chan
authors.forename: Han Kiat
authors.initials: HK
authors.lastname: Ho
authors.forename: Giorgia
authors.initials: G
authors.lastname: Pastorin
authors.forename: Wee Han
authors.initials: WH
authors.lastname: Ang
mesh_terms: D000906:Antibodies / Q000737:chemistry; D001681:Biological Assay; D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry; D004076:Digoxigenin / Q000737:chemistry; D006640:Histidine Decarboxylase / Q000032:analysis / Q000235:genetics; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D010957:Plasmids; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction / Q000379:methods*; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry
chemical_list: D000906:Antibodies; D017931:DNA Primers; D001710:Biotin; D004247:DNA; D019809:Streptavidin; D006640:Histidine Decarboxylase; D004076:Digoxigenin