title: Jacaric acid, a linolenic acid isomer with a conjugated triene system, has a strong antitumor effect in vitro and in vivo.
abstract: In this study, we compared the cytotoxic effects of natural conjugated linolenic acids (CLnAs) on human adenocarcinoma cells (DLD-1) in vitro, with the goal of finding CLnA isomers with strong cytotoxic effects. The antitumor effect of the CLnA with the strongest cytotoxic effect was then examined in mice. The results showed that all CLnA isomers have strong cytotoxic effects on DLD-1 cells, with jacaric acid (JA) having the strongest effect. Examination of the mechanism of cell death showed that CLnAs induce apoptosis in DLD-1 cells via lipid peroxidation. The intracellular levels of incorporated CLnAs were measured to examine the reason for differences in cytotoxic effects. These results showed that JA was taken into cells efficiently. Collectively, these results suggest that the cytotoxic effect of CLnAs is dependent on intracellular incorporation and induction of apoptosis via lipid peroxidation. JA also had a strong preventive antitumor effect in vivo in nude mice into which DLD-1 cells were transplanted. These results suggest that JA can be used as a dietary constituent for prevention of cancer.
authors.affiliation: Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Japan.
authors.forename: Nahoko
authors.initials: N
authors.lastname: Shinohara
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tsuduki
authors.forename: Junya
authors.initials: J
authors.lastname: Ito
authors.forename: Taro
authors.initials: T
authors.lastname: Honma
authors.forename: Ryo
authors.initials: R
authors.lastname: Kijima
authors.forename: Soko
authors.initials: S
authors.lastname: Sugawara
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Arai
authors.forename: Masao
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Aya
authors.initials: A
authors.lastname: Ikezaki
authors.forename: Marino
authors.initials: M
authors.lastname: Yokoyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nishiyama
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
authors.forename: Ikuo
authors.initials: I
authors.lastname: Ikeda
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001692:Biological Transport / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007536:Isomerism; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D017962:alpha-Linolenic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: D017962:alpha-Linolenic Acid