title: Protective effect of blackcurrant on liver cell membrane of rats intoxicated with ethanol.
abstract: Chronic ethanol intoxication oxidative stress participates in the development of many diseases. Nutrition and the interaction of food nutrients with ethanol metabolism may modulate alcohol toxicity. One such compound is blackcurrant, which also has antioxidant abilities. We investigated the effect of blackcurrant as an antioxidant on the composition and electrical charge of liver cell membranes in ethanol-intoxicated rats. Qualitative and quantitative phospholipid composition and the presence of integral membrane proteins were determined by high-performance liquid chromatography. Electrophoresis was used to determine the surface charge density of the rat liver cell membranes. Ethanol intoxication is characterized by changes in cell metabolism that alter the structure and function of cell membrane components. Ethanol increased phospholipid levels and altered the level of integral proteins as determined by decreased phenylalanine, cysteine, and lysine. Ethanol significantly enhanced changes in the surface charge density of the liver cell membranes. Administration of blackcurrant to rats intoxicated with ethanol significantly protected lipids and proteins against oxidative modifications. It is possible that the beneficial effect of blackcurrant is connected with its abilities to scavenge free radicals and to chelate metal ions.
authors.affiliation: Institute of Chemistry, University in Białystok, Al. Piłsudskiego 11/4, 15-443 Białystok, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Szachowicz-Petelska
authors.forename: Izabela
authors.initials: I
authors.lastname: Dobrzyńska
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Skrzydlewska
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Figaszewski
mesh_terms: D000435:Alcoholic Intoxication / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D002478:Cells, Cultured; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome; D017965:gamma-Linolenic Acid / Q000494:pharmacology*
chemical_list: D017965:gamma-Linolenic Acid; C084865:black currant oil