title: Isolation of bioactive components from Flaveria bidentis (L.) Kuntze using high-speed counter-current chromatography and time-controlled collection method.
abstract: Semipreparative high-speed counter-current chromatography (HSCCC) by time-controlled collection method was successfully applied for isolation and purification of α-terthienyl, 5-(3-buten-1-ynyl)-2,2'-bithienyl, and 5-(3-penten-1-ynyl)-2,2'-bithienyl from Flaveria bidentis (L.) Kuntze for the first time. The two-phase solvent system composed of n-hexane and acetonitrile at the volume ratio of 1:1 (v/v) was used for the semipreparative HSCCC. The 5.2 mg α-terthienyl, 2.2 mg 5-(3-buten-1-ynyl)-2,2'-bithienyl, and 4.3 mg 5-(3-penten-1-ynyl)-2,2'-bithienyl with the purity of 99.9, 90.2, and 92.1% were produced from 265.6 mg crude extract, respectively, and 5-(3-penten-1-ynyl)-2,2'-bithienyl was first isolated from Flaveria bidentis (L.) Kuntze. The structures of the separated compounds were identified by electrospray-ionization mass spectrometry and proton and carbon nuclear magnetic resonance ((1)H- and (13)C-NMR).
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, P. R. China. weiyun@mail.buct.edu.cn
authors.forename: Yun
authors.initials: Y
authors.lastname: Wei
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Yin
authors.forename: Jilin
authors.initials: J
authors.lastname: Du
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhang
mesh_terms: D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D037142:Flaveria / Q000737:chemistry*; D059625:Liquid-Liquid Extraction / Q000295:instrumentation / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D010936:Plant Extracts