title: An endoglycosidase with alternative glycan specificity allows broadened glycoprotein remodelling.
abstract: Protein endoglycosidases are useful for biocatalytic alteration of glycans on protein surfaces, but the currently limited selectivity of endoglycosidases has prevented effective manipulation of certain N-linked glycans widely found in nature. Here we reveal that a bacterial endoglycosidase from  Streptococcus pyogenes , EndoS, is complementary to other known endoglycosidases (EndoA, EndoH) used for current protein remodeling. It allows processing of complex-type N-linked glycans +/- core fucosylation but does not process oligomannose- or hybrid-type glycans. This biocatalytic activity now addresses previously refractory antibody glycoforms.
authors.affiliation: Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK.
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Goodfellow
authors.forename: Kavitha
authors.initials: K
authors.lastname: Baruah
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Camille
authors.initials: C
authors.lastname: Bonomelli
authors.forename: Benjamin
authors.initials: B
authors.lastname: Krishna
authors.forename: David J
authors.initials: DJ
authors.lastname: Harvey
authors.forename: Max
authors.initials: M
authors.lastname: Crispin
authors.forename: Christopher N
authors.initials: CN
authors.lastname: Scanlan
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Davis
mesh_terms: D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D011134:Polysaccharides / Q000378:metabolism*; D011487:Protein Conformation; D013297:Streptococcus pyogenes / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D006023:Glycoproteins; D007074:Immunoglobulin G; D011134:Polysaccharides; D006026:Glycoside Hydrolases