title: Histological staining of amyloid and pre-amyloid peptides and proteins in mouse tissue.
abstract: The increased availability of transgenic mouse models for studying human diseases has shifted the focus of many laboratories from in vitro to in vivo assays. Herein, methods are described to allow investigators to obtain well-preserved mouse tissue to be stained with the standard histological dyes for amyloid, Congo Red, and Thioflavin S. These sections can as well be used for immunohistological procedures that allow detection of tissue amyloid and pre-amyloid, such as those composed of the amyloid-β peptide, the tau protein, and the islet amyloid polypeptide.
authors.affiliation: Department of Physiology, New York University School of Medicine, New York, NY, USA.
authors.forename: Hameetha B
authors.initials: HB
authors.lastname: Rajamohamedsait
authors.forename: Einar M
authors.initials: EM
authors.lastname: Sigurdsson
mesh_terms: D016229:Amyloid beta-Peptides / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D052160:Benzothiazoles; D004396:Coloring Agents / Q000378:metabolism; D003224:Congo Red / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D058228:Islet Amyloid Polypeptide / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D010477:Perfusion; D013194:Staining and Labeling / Q000379:methods*; D013844:Thiazoles / Q000378:metabolism; D016875:tau Proteins / Q000276:immunology / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D000911:Antibodies, Monoclonal; D052160:Benzothiazoles; D004396:Coloring Agents; D058228:Islet Amyloid Polypeptide; D013844:Thiazoles; D016875:tau Proteins; C009462:thioflavin T; D003224:Congo Red