title: [Tumor necrosis factor-α enhances radiosensitivity of A549 cells].
abstract: OBJECTIVE
  To assess the effects of tumor necrosis factor-α (TNF-α) in enhancing the radiosensitivity of lung cancer cells in vitro.
  
  
  METHODS
  A549 cells were exposed to γ-ray with or without TNF-α treatment. MTT assay was used to evaluate the cell viability, and flow cytometry was performed to assess the cell apoptosis. Western blotting was used to observe the expression of caspase-3 protein in the exposed cells.
  
  
  RESULTS
  Compared with the exposed cells without TNF-α treatment, the cells treated with TNF-α showed significantly suppressed cell proliferation, increased the cell apoptosis, altered cell cycle, and increased caspase-3 protein expression after γ-ray exposure.
  
  
  CONCLUSIONS
  TNF-α can enhance the radiosensitivity of A549 cells to increase the efficiency of radiotherapy with γ-ray irradiation.
authors.affiliation: Department of Cardiothoracic Surgery, First Affiliated Hospital of General Hospital of PLA, Beijing 100048, China. 304xiahui@sina.com
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.forename: Changhai
authors.initials: C
authors.lastname: Yu
authors.forename: Yiming
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jianqi
authors.initials: J
authors.lastname: Yu
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D002453:Cell Cycle / Q000187:drug effects* / Q000528:radiation effects; D045744:Cell Line, Tumor; D005720:Gamma Rays; D006801:Humans; D008175:Lung Neoplasms; D011836:Radiation Tolerance / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D014409:Tumor Necrosis Factor-alpha