title: The utility of mitochondrial DNA fragments for genetic identification of forensically important sarcophagid flies (Diptera: Sarcophagidae) in China.
abstract: Species-diagnostic anatomical characters of fleshflies are not known for most   immature stages or even adults, and an existing key may be incomplete or difûcult for   nonspecialists to use. The use of sarcophagids for PMI estimations has been greatly hampered   by their highly similar morphological characters. DNA-based method can be used as a   supplemental means of morphological method in identification of forensically important   sarcophagid flies. However, relying solely on single DNA fragment for delimiting species is   considered to be unreliable, especially when the fragment was small. Sequence data of   selected regions of the cytochrome oxidase subunit two (COII) and 16S ribosomal RNA (16S   rRNA) genes of the most important Chinese fleshfly taxa associated with cadavers are   presented, which can be instrumental for implementation of the Chinese Sarcophagidae   database. Phylogenetic analysis of the sequenced segments showed that all sarcophagid   specimens were properly assigned into five species, which indicated the possibility of   separation congeneric species with the short fragments.
authors.affiliation: Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, Hunan, China.
authors.forename: Y D
authors.initials: YD
authors.lastname: Guo
authors.forename: J F
authors.initials: JF
authors.lastname: Cai
authors.forename: F
authors.initials: F
authors.lastname: Xiong
authors.forename: H J
authors.initials: HJ
authors.lastname: Wang
authors.forename: J F
authors.initials: JF
authors.lastname: Wen
authors.forename: J B
authors.initials: JB
authors.lastname: Li
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002681:China; D016172:DNA Fingerprinting / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D004772:Entomology / Q000379:methods*; D044707:Forensic Sciences / Q000379:methods*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058493:Sarcophagidae / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D003576:Electron Transport Complex IV