title: Culture and PCR detection of Haemophilus influenzae and Haemophilus haemolyticus in Australian Indigenous children with bronchiectasis.
abstract: A PCR for protein D (hpd#3) was used to differentiate nontypeable Haemophilus influenzae (NTHI) from Haemophilus haemolyticus. While 90% of nasopharyngeal specimens and 100% of lower-airway specimens from 84 Indigenous Australian children with bronchiectasis had phenotypic NTHI isolates confirmed as H. influenzae, only 39% of oropharyngeal specimens with phenotypic NTHI had H. influenzae. The nasopharynx is therefore the preferred site for NTHI colonization studies, and NTHI is confirmed as an important lower-airway pathogen.
authors.affiliation: Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia. kim.hare@menzies.edu.au
authors.forename: K M
authors.initials: KM
authors.lastname: Hare
authors.forename: M J
authors.initials: MJ
authors.lastname: Binks
authors.forename: K
authors.initials: K
authors.lastname: Grimwood
authors.forename: A B
authors.initials: AB
authors.lastname: Chang
authors.forename: A J
authors.initials: AJ
authors.lastname: Leach
authors.forename: H
authors.initials: H
authors.lastname: Smith-Vaughan
mesh_terms: D001315:Australia; D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D001987:Bronchiectasis / Q000150:complications*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006190:Haemophilus / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006192:Haemophilus Infections / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D007223:Infant; D008074:Lipoproteins / Q000235:genetics; D008297:Male; D009305:Nasopharynx / Q000382:microbiology; D009960:Oropharynx / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D044382:Population Groups; D012137:Respiratory System / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins