title: Impregnation of plasmid DNA into three-dimensional PLGA scaffold enhances DNA expression of mesenchymal stem cells in vitro.
abstract: Current efforts had been made to undertake a three-dimensional (3-D) reverse transfection of bone marrow-derived mesenchymal stem cells (BM-MSCs) in PLGA scaffolds. As a kind of multipotent stem cells, BM-MSCs show great potential and tremendous capacity in the gene transfection field and PLGA 3-D scaffold has been shown to be a biomaterial that provides structural support to cells proliferation and tissue engineering. The objective of this study was to assess the transfection efficiency of BM-MSCs with a 3-D reverse transfection method by using PLGA scaffold and observe the SEM photographs of BM-MSCs cultured on PLGA scaffold. BM-MSCs were cultured in 3-D PLGA scaffold which was incorporated with pullulan-spermine/pGL3. It was shown that the gene expression duration of BM-MSCs transfected using 3D reverse method with pullulan-spermine/DNA in the presence of serum maintained 12 days at high levels as compared with the plasmid DNA in medium, and scanning electronic microscopy (SEM) photographs of BM-MSCs cultured on PLGA scaffold exhibited robust cell attachment and viability when cultured in PLGA scaffold in vitro. This study demonstrates that the 3-D reverse transfection method of BM-MSCs using PLGA scaffold could achieve long gene expression in a relatively high level, therefore this transfection system is promising in gene transfection and tissue engineering.
authors.affiliation: Department of Pharmacy, Zhejiang Provincial Corps Hospital of Chinese People's Armed Police Forces, Jiaxing, PR China.
authors.forename: Pei-Hong
authors.initials: PH
authors.lastname: Miao
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: He
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Hu
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Gao
authors.forename: Zhong-Jie
authors.initials: ZJ
authors.lastname: Hu
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry*; D005079:Excipients; D005936:Glucans / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010957:Plasmids / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D013096:Spermine / Q000737:chemistry; D014162:Transfection / Q000379:methods
chemical_list: D005079:Excipients; D005936:Glucans; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D013096:Spermine; D019344:Lactic Acid; C009109:pullulan; D004247:DNA