title: Developing an antibody-binding protein cage as a molecular recognition drug modular nanoplatform.
abstract: We genetically introduced the Fc-binding peptide (FcBP) into the loop of a self-assembled protein cage, ferritin, constituting four-fold symmetry at the surface to use it as a modular delivery nanoplatform. FcBP-presenting ferritin (FcBP-ferritin) formed very stable non-covalent complexes with both human and rabbit IgGs through the simple molecular recognition between the Fc region of the antibodies and the Fc-binding peptide clusters inserted onto the surface of FcBP-ferritin. This approach realized orientation-controlled display of antibodies on the surfaces of the protein cages simply by mixing without any complicated chemical conjugation. Using trastuzumab, a human anti-HER2 antibody used to treat patients with breast cancer, and a rabbit antibody to folate receptor, along with fluorescently labeled FcBP-ferritin, we demonstrated the specific binding of these complexes to breast cancer cells and folate receptor over-expressing cells, respectively, by fluorescent cell imaging. FcBP-ferritin may be potentially used as modular nanoplatforms for active targeted delivery vehicles or molecular imaging probes with a series of antibodies on demand.
authors.affiliation: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) Daejeon 305-806, Republic of Korea.
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Kang
authors.forename: Young Ji
authors.initials: YJ
authors.lastname: Kang
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Shin
authors.forename: Sang J
authors.initials: SJ
authors.lastname: Chung
authors.forename: Sebyung
authors.initials: S
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology*; D045744:Cell Line, Tumor; D002850:Chromatography, Gel; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D011817:Rabbits
chemical_list: D000906:Antibodies; D002352:Carrier Proteins; D010455:Peptides