title: Two-dimensional proteome reference map of Rhizobium tropici PRF 81 reveals several symbiotic determinants and strong resemblance with agrobacteria.
abstract: Rhizobium tropici strain PRF 81 is used in commercial inoculants for common-bean crops in Brazil because of its high efficiency in nitrogen fixation and, as in other strains belonging to this species, its tolerance of environmental stresses, representing a useful biological alternative to chemical nitrogen fertilizers. In this study, a proteomic reference map of PRF 81 was obtained by two-dimensional gel electrophoresis and MALDI-TOF/TOF-TOF mass spectrometry. In total, 115 spots representing 109 different proteins were successfully identified, contributing to a better understanding of the rhizobia-legume symbiosis and supporting, at proteomics level, a strong resemblance with agrobacteria.
authors.affiliation: Embrapa Soja, Londrina, PR, Brazil.
authors.forename: Douglas Fabiano
authors.initials: DF
authors.lastname: Gomes
authors.forename: Jesiane Stefania da Silva
authors.initials: JS
authors.lastname: Batista
authors.forename: Adalgisa Ribeiro
authors.initials: AR
authors.lastname: Torres
authors.forename: Diva
authors.initials: D
authors.lastname: de Souza Andrade
authors.forename: Lygia Vitoria
authors.initials: LV
authors.lastname: Galli-Terasawa
authors.forename: Mariangela
authors.initials: M
authors.lastname: Hungria
mesh_terms: D060054:Agrobacterium / Q000737:chemistry*; D001426:Bacterial Proteins / Q000032:analysis*; D018556:Crops, Agricultural / Q000382:microbiology; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D042162:Rhizobium tropici / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013559:Symbiosis
chemical_list: D001426:Bacterial Proteins; D020543:Proteome