title: Comparison of cardiac stem cells and mesenchymal stem cells transplantation on the cardiac electrophysiology in rats with myocardial infarction.
abstract: BACKGROUND
  Whether transplanted cardiac stem cells (CSCs) and mesenchymal stem cells (MSCs) improved ventricular fibrillation threshold (VFT) similarly is still unclear. We sought to compare the effects of the CSC and MSC transplantation on the electrophysiological characteristics and VFT in rats with myocardial infarction (MI).
  
  
  METHODS
  MI was induced in 30 male Sprague-Dawley rats. Two weeks later, animals were randomized to receive 5 × 10(6) CSCs labeled with PKH26 in PBS or 5 × 10(6) MSCs labeled with PKH26 in phosphate buffer solution(PBS) or PBS alone injection into the infarcted anterior ventricular free wall. Six weeks after the injection, electrophysiological characteristics and VFT were measured. Labeled CSCs and MSCs were observed in 5 μm cryostat sections from each heart.
  
  
  RESULTS
  Malignant ventricular arrhythmias were significantly (P = 0.0055) less inducible in the CSC group than the MSC group. The VFTs were improved in the CSC group compared with the MSC group. Labeled CSCs and MSCs were identified in the infarct zone and infarct marginal zone. Labeled CSCs expressed Connexin-43, von Willebrand factor, α-smooth muscle actin and α-sarcomeric actin,while the Labeled MSCs expressed von Willebrand factor, α-smooth muscle actin and α-sarcomeric actin in vivo.
  
  
  CONCLUSIONS
  After 6 weeks of cell transplantation, CSCs are superior to MSCs in modulating the electrophysiological abnormality and improving the VFT in rats with MI. CSCs and MSCs express markers that suggest muscle, endothelium and vascular smooth muscle phenotypes in vivo, but MSCs rarely express Connexin-43.
authors.affiliation: Cardiovascular Medicine, The Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, 107 Yanjiang Xi Road, Guangzhou, 510120, China.
authors.forename: Shao-Xin
authors.initials: SX
authors.lastname: Zheng
authors.forename: Yin-Lun
authors.initials: YL
authors.lastname: Weng
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Zhou
authors.forename: Zhu-Zhi
authors.initials: ZZ
authors.lastname: Wen
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Jing-Feng
authors.initials: JF
authors.lastname: Wang
authors.forename: Tong
authors.initials: T
authors.lastname: Wang
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000628:therapy; D053840:Brugada Syndrome; D000075224:Cardiac Conduction System Disease; D054849:Cardiac Electrophysiology; D002454:Cell Differentiation; D064987:Cell- and Tissue-Based Therapy; D018031:Connexin 43 / Q000096:biosynthesis; D006321:Heart / Q000502:physiology / Q000503:physiopathology; D006329:Heart Conduction System / Q000002:abnormalities; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D009203:Myocardial Infarction / Q000628:therapy*; D032383:Myocytes, Cardiac; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells; D014693:Ventricular Fibrillation / Q000628:therapy*; D014841:von Willebrand Factor / Q000096:biosynthesis
chemical_list: D000199:Actins; D018031:Connexin 43; D014841:von Willebrand Factor