title: Rapid and specific detection of Lassa virus by reverse transcription-PCR coupled with oligonucleotide array hybridization.
abstract: To facilitate sequence-specific detection of DNA amplified in a diagnostic reverse transcription (RT)-PCR for Lassa virus, we developed an array featuring 47 oligonucleotide probes for post-PCR hybridization of the amplicons. The array procedure may be performed with low-tech equipment and does not take longer than agarose gel detection.
authors.affiliation: Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Olschläger
authors.forename: Stephan
authors.initials: S
authors.lastname: Günther
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007835:Lassa Fever / Q000175:diagnosis* / Q000821:virology; D007836:Lassa virus / Q000235:genetics / Q000302:isolation & purification*; D046228:Microarray Analysis / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D014773:Virology / Q000379:methods*
chemical_list: D015345:Oligonucleotide Probes