title: Polyvalent display of RGD motifs on turnip yellow mosaic virus for enhanced stem cell adhesion and spreading.
abstract: Turnip yellow mosaic virus (TYMV) is a stable 28 nm icosahedral plant virus that can be isolated in gram quantities. In order to study the polyvalent effect of Arg-Gly-Asp (RGD) clustering on the response of bone marrow stem cells (BMSCs), an RGD motif was genetically displayed on the coat protein of the TYMV capsid. Composite films composed of either wild-type TYMV or TYMV-RGD44, in combination with poly(allylamine hydrochloride) (PAH), were fabricated by a layer-by-layer adsorption of virus and PAH. The deposition process was studied by quartz crystal microbalance, UV-visible spectroscopy and atomic force microscopy. BMSC adhesion assays showed enhanced cell adhesion and spreading on TYMV-RGD44 coated substrates compared to native TYMV. These results demonstrate the potential of TYMV as a viable scaffold for bioactive peptide display and cell culturing studies.
authors.affiliation: Department of Chemistry and Biochemistry and Nanocenter, University of South Carolina, Columbia, SC 29208, USA.
authors.forename: Xingjie
authors.initials: X
authors.lastname: Zan
authors.forename: Pongkwan
authors.initials: P
authors.lastname: Sitasuwan
authors.forename: Joshua
authors.initials: J
authors.lastname: Powell
authors.forename: Theo W
authors.initials: TW
authors.lastname: Dreher
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008297:Male; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000494:pharmacology*; D011073:Polyamines / Q000494:pharmacology; D058525:Quartz Crystal Microbalance Techniques; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D017806:Tymovirus / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D014461:Ultracentrifugation
chemical_list: D009842:Oligopeptides; D011073:Polyamines; C063994:polyallylamine; C047981:arginyl-glycyl-aspartic acid