title: Dendrimer type bio-reducible polymer for efficient gene delivery.
abstract: Arginine-grafted bio-reducible poly(disulfide amine) (ABP) was incorporated into the poly(amido amine) (PAMAM) dendrimer, creating a high molecular weight bio-reducible polymer, PAM-ABP, to overcome the limitations of the low molecular weight ABP. The newly synthesized PAM-ABP was studied to determine its efficacy as a gene delivery carrier. The PAM-ABP demonstrated superior condensing ability for plasmid DNA through the formation of compact nanosized polyplexes. These compact polyplexes enhanced cellular uptake and were less susceptible to reducing agents, resulting in greater transfection efficiency compared to ABP alone. Based on these results, this newly developed PAM-ABP polyplex is a promising delivery system for clinical gene therapy.
authors.affiliation: Center for Controlled Chemical Delivery, Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT 84112, United States.
authors.forename: Hye Yeong
authors.initials: HY
authors.lastname: Nam
authors.forename: Kihoon
authors.initials: K
authors.lastname: Nam
authors.forename: Minhyung
authors.initials: M
authors.lastname: Lee
authors.forename: Sung Wan
authors.initials: SW
authors.lastname: Kim
authors.forename: David A
authors.initials: DA
authors.lastname: Bull
mesh_terms: D000818:Animals; D001120:Arginine / Q000008:administration & dosage* / Q000737:chemistry; D001583:Benzoxazoles / Q000008:administration & dosage / Q000737:chemistry; D019328:Buthionine Sulfoximine; D002460:Cell Line; D002470:Cell Survival; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry; D050091:Dendrimers / Q000008:administration & dosage* / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D010957:Plasmids; D011073:Polyamines / Q000008:administration & dosage* / Q000737:chemistry; D011806:Quinolinium Compounds / Q000008:administration & dosage / Q000737:chemistry; D014162:Transfection / Q000379:methods*
chemical_list: D001583:Benzoxazoles; D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines; D011806:Quinolinium Compounds; C534803:arginine-grafted bioreducible poly(disulfide amine)polymer; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium; D049452:Green Fluorescent Proteins; D019328:Buthionine Sulfoximine; D004247:DNA; D001120:Arginine