title: Effect of oxidized regenerated cellulose on the healing of pharyngeal wound: an experimental animal study.
abstract: BACKGROUND
  This study aimed to investigate the relationship between oxidized regenerated cellulose (ORC) and mucosa healing in an experimental animal model.
  
  
  METHODS
  Fifteen adult Sprague-Dawley rats were randomly divided into three groups that underwent different wound treatments. In Group 1, no pharyngeal wound was created. In Group 2, the pharyngeal wound was sutured with Prolene only. In Group 3, the pharyngeal wound was sutured with Prolene, and covered with one layer of ORC before closure of the skin wound. The animals were euthanized either 5 or 10 days after operation, and wound conditions were inspected and recorded. Specimens including sections of larynx and pharynx/upper esophagus were taken for microscopic and molecular biological examination.
  
  
  RESULTS
  The pharyngotomy/esophagotomy wounds achieved good healing outcomes 10 days after operation. Wounds treated with ORC had significantly diminished inflammatory cell infiltration in microscopic examination when compared with that of those without ORC 5 days after operation. The matrix metalloproteinases (MMP) expression level was higher in wounds of Group 2 and Group 3, when compared with that of group 1. In addition, the MMP expression level was lower in the ORC-treated wounds when compared with that of those without ORC. There was no significant difference in fibroblast proliferation, collagen deposition, endothelin-1, alpha-smooth muscle actin, and transforming growth factor beta 1 expression level between wounds treated with ORC and those without ORC.
  
  
  CONCLUSIONS
  Reduced inflammatory response and decreased MMP expression level was observed in ORC-treated wounds. Whether ORC facilitates mucosa healing requires further investigation.
authors.affiliation: Department of Otolaryngology, Taichung Veterans General Hospital, Taichung, Taiwan, ROC.
authors.forename: Shih-An
authors.initials: SA
authors.lastname: Liu
authors.forename: Ching-Chang
authors.initials: CC
authors.lastname: Cheng
authors.forename: Jiun-Sheng
authors.initials: JS
authors.lastname: Chen
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Hung
authors.forename: Fun-Jou
authors.initials: FJ
authors.lastname: Chen
authors.forename: Yung-Tsung
authors.initials: YT
authors.lastname: Chiu
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D002483:Cellulose, Oxidized / Q000494:pharmacology*; D019332:Endothelin-1 / Q000032:analysis; D004947:Esophagus / Q000473:pathology / Q000601:surgery*; D020782:Matrix Metalloproteinases / Q000032:analysis; D010614:Pharynx / Q000473:pathology / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000199:Actins; D002483:Cellulose, Oxidized; D019332:Endothelin-1; D020782:Matrix Metalloproteinases; D053773:Transforming Growth Factor beta1; C498862:Acta2 protein, rat