title: Proteomic and redox-proteomic analysis of berberine-induced cytotoxicity in breast cancer cells.
abstract: Berberine is a natural product isolated from herbal plants such as Rhizoma coptidis which has been shown to have anti-neoplastic properties. However, the effects of berberine on the behavior of breast cancers are largely unknown. To determine if berberine might be useful in the treatment of breast cancer and its cytotoxic mechanism, we analyzed the impact of berberine treatment on differential protein expression and redox regulation in human breast cancer cell line MCF-7 using lysine- and cysteine-labeling two-dimensional difference gel electrophoresis (2D-DIGE) combined with mass spectrometry (MS). This study demonstrated that 96 and 22 protein features were significantly changed in protein expression and thiol reactivity, respectively and revealed that berberine-induced cytotoxicity in breast cancer cells involves dysregulation of protein folding, proteolysis, redox regulation, protein trafficking, cell signaling, electron transport, metabolism and centrosomal structure. Our work shows that this combined proteomic strategy provides a rapid method to study the molecular mechanisms of berberine-induced cytotoxicity in breast cancer cells. The identified targets may be useful for further evaluation as potential targets in breast cancer therapy.
authors.affiliation: Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan.
authors.forename: Hsiu-Chuan
authors.initials: HC
authors.lastname: Chou
authors.forename: Ying-Chieh
authors.initials: YC
authors.lastname: Lu
authors.forename: Chao-Sheng
authors.initials: CS
authors.lastname: Cheng
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Chen
authors.forename: Ping-Chiang
authors.initials: PC
authors.lastname: Lyu
authors.forename: Cheng-Wen
authors.initials: CW
authors.lastname: Lin
authors.forename: John F
authors.initials: JF
authors.lastname: Timms
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Chan
mesh_terms: D001599:Berberine / Q000737:chemistry / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D030021:Pinellia / Q000737:chemistry; D040901:Proteomics
chemical_list: D003603:Cytotoxins; D009363:Neoplasm Proteins; D001599:Berberine