title: The effect of conventional and mini-invasive cardiopulmonary bypass on neutrophil activation in patients undergoing coronary artery bypass grafting.
abstract: Interleukin-10 (IL-10) is considered to be a cytokine with potent anti-inflammatory properties, which have been previously linked to increased incidence of sepsis. The level of IL-10 is elevated by cardiac surgery when cardiopulmonary bypass (CPB) and methylprednisolone are used. In our study, we compare the level of IL-10, IL-10 Receptor (IL-10R), and percentage of neutrophils between two groups of cardiac surgical patients undergoing Coronary Artery Bypass Grafting, both of which were not given methylprednisolone. The first group was operated with conventional CPB, while the second group was operated with minimally invasive CPB (mini-CPB). We detected enhanced level of IL-10 during surgery and at the end of surgery in both groups of patients. While no correlation between IL-10 and IL10R was found, IL-10 was positively correlated with increased percentage of neutrophils at the time points when the level of IL-10 peaked.
authors.affiliation: Department of Clinical Immunology, Faculty of Medicine, Charles University, 50005 Hradec Kralove, Czech Republic. kolackovam@lfhk.cuni.cz
authors.forename: Martina
authors.initials: M
authors.lastname: Kolackova
authors.forename: Jan
authors.initials: J
authors.lastname: Krejsek
authors.forename: Vladimir
authors.initials: V
authors.lastname: Svitek
authors.forename: Pavel
authors.initials: P
authors.lastname: Kunes
authors.forename: Jiri
authors.initials: J
authors.lastname: Mandak
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Holubcova
authors.forename: Vladimir
authors.initials: V
authors.lastname: Lonsky
mesh_terms: D000368:Aged; D002315:Cardiopulmonary Bypass / Q000379:methods*; D001026:Coronary Artery Bypass / Q000379:methods*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology; D008875:Middle Aged; D019060:Minimally Invasive Surgical Procedures; D018375:Neutrophil Activation; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism*; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D017472:Receptors, Interleukin-1; D016753:Interleukin-10; D008775:Methylprednisolone