title: Brevibacterium yomogidense sp. nov., isolated from a soil conditioner made from poultry manure.
abstract: A novel Gram-stain-positive rod-shaped actinobacterium was isolated from a soil conditioner made from poultry manure. The isolate, designated strain MN-6-a(T), contained anteiso-C(15 : 0) and anteiso-C(17 : 0) as the major fatty acids, and MK-7(H(2)) and MK-8(H(2)) as the major menaquinones. Phosphatidylglycerol was a major polar lipid. The G+C content of the genomic DNA was 67.4 mol%. Phylogenetic analysis showed that strain MN-6-a(T) was closely related to Brevibacterium salitolerans TRM 415(T) with 97.1 % 16S rRNA gene sequence similarity. DNA-DNA hybridization showed that strain MN-6-a(T) had 10.2 % genomic relatedness with B. salitolerans TRM 415(T). On the basis of phenotypic, phylogenetic and chemotaxonomic data obtained in this study, strain MN-6-a(T) represents a novel species of the genus Brevibacterium, for which the name Brevibacterium yomogidense sp. nov. is proposed. The type strain is MN-6-a(T) ( = JCM 17779(T) = DSM 24850(T)).
authors.affiliation: Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori, 036-8561, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Tonouchi
authors.affiliation: Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori, 036-8561, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kitamura
authors.affiliation: Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori, 036-8561, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujita
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001951:Brevibacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008372:Manure / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011200:Poultry / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D008372:Manure; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8; C062629:menaquinone 7