title: [Effect of schizandrin B on H(2)O(2)-induced apoptosis of human hepatocytes in vitro: role of Fas pathway].
abstract: OBJECTIVE
  To investigate the role of Fas pathway in H(2)O(2)-induced apoptosis of L02 human hepatocytes and the effect of schisandrin B on Fas pathway.
  
  
  METHODS
  Real-time quantitative PCR was used to detect the expressions of FAS, fas associated death domain protein (FADD) and caspase-8 mRNA in L02 cells exposed to H(2)O(2). Flow cytometry was employed to assess the cell apoptosis. ELISA, Western blotting and spectrophotometric assay were performed to determine the expressions of FAS protein, FADD protein and caspase-8 activity.
  
  
  RESULTS
  Within the dose range of 5-15 mol/L, schisandrin B dose-dependently inhibited FAS and FADD expressions and caspase-8 activation.
  
  
  CONCLUSIONS
  Schisandrin B can partially inhibit H(2)O(2)-induced L02 cell apoptosis possibly by affecting the FAS-FADD-caspase-8 pathway.
authors.affiliation: Department of Pharmacy, Nanfang Hospital, School of Pharmaceutical Sciences3, Southern Medical University, Guangzhou 510515, China. wantoflyinsky@gmail.com
authors.forename: Jing
authors.initials: J
authors.lastname: Cai
authors.forename: Qiaobing
authors.initials: Q
authors.lastname: Huang
authors.forename: Debiao
authors.initials: D
authors.lastname: Chi
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053181:Caspase 8 / Q000378:metabolism; D002460:Cell Line; D034242:Cyclooctanes / Q000494:pharmacology; D053200:Fas-Associated Death Domain Protein / Q000378:metabolism*; D005434:Flow Cytometry; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects; D017705:Lignans / Q000494:pharmacology*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D015398:Signal Transduction; D019014:fas Receptor / Q000378:metabolism
chemical_list: D034242:Cyclooctanes; C505533:FADD protein, human; C491901:FAS protein, human; D053200:Fas-Associated Death Domain Protein; D017705:Lignans; D011083:Polycyclic Compounds; D019014:fas Receptor; C015499:schizandrin B; D006861:Hydrogen Peroxide; C505530:CASP8 protein, human; D053181:Caspase 8