title: Pilot-scale ethanol production from rice straw hydrolysates using xylose-fermenting Pichia stipitis.
abstract: Ethanol was produced at pilot scale from rice straw hydrolysates using a Pichia stipitis strain previously adapted to NaOH-neutralized hydrolysates. The highest ethanol yield was 0.44 ± 0.02 g(p)/g(s) at an aeration rate of 0.05 vvm using overliming-detoxified hydrolysates. The yield with hydrolysates conditioned by ammonia and NaOH was 0.39 ± 0.01 and 0.34 ± 0.01 g(p)/g(s), respectively, were achieved at the same aeration rate. The actual ethanol yield from hydrolysate fermentation with ammonia neutralization was similar to that with overliming hydrolysate after taking into account the xylose loss resulting from these conditioning processes. Moreover, the ethanol yield from ammonia-neutralized hydrolysates could be further enhanced by increasing the initial cell density by two-fold or reducing the combined concentration of furfural and 5-hydroxymethyl furfural to 0.6g/L by reducing the severity of operational conditions in pretreatment. This study demonstrated the potential for commercial ethanol production from rice straw via xylose fermentation.
authors.affiliation: Department of Life Sciences, National Central University, No 300, Jhongda Rd, Jhongli City, Taoyuan County 32001, Taiwan, ROC.
authors.forename: Ting-Hsiang
authors.initials: TH
authors.lastname: Lin
authors.forename: Chiung-Fang
authors.initials: CF
authors.lastname: Huang
authors.forename: Gia-Luen
authors.initials: GL
authors.lastname: Guo
authors.forename: Wen-Song
authors.initials: WS
authors.lastname: Hwang
authors.forename: Shir-Ly
authors.initials: SL
authors.lastname: Huang
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D000641:Ammonia / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects / Q000502:physiology*; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism; D012275:Oryza / Q000737:chemistry*; D010843:Pichia / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D010865:Pilot Projects; D012972:Sodium Hydroxide / Q000494:pharmacology; D014866:Waste Products / Q000032:analysis*; D014994:Xylose / Q000378:metabolism*
chemical_list: D014866:Waste Products; C036909:lignocellulose; D000431:Ethanol; D012972:Sodium Hydroxide; C008046:5-hydroxymethylfurfural; D000641:Ammonia; D008031:Lignin; D014994:Xylose; D005662:Furaldehyde