title: Alternative oxidase modulates leaf mitochondrial concentrations of superoxide and nitric oxide.
abstract: • The nonenergy-conserving alternative oxidase (AOX) has been hypothesized to modulate the amount of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in plant mitochondria but there is sparse direct in planta evidence to support this. • Laser scanning fluorescent confocal microscopy and biochemical methods were used to directly estimate in planta leaf concentrations of superoxide (O2(-)), nitric oxide (NO), peroxynitrite (ONOO(-)) and hydrogen peroxide (H(2)O(2)) in wildtype (Wt) tobacco (Nicotiana tabacum) and transgenic tobacco with altered amounts of AOX. • We found that plants lacking AOX have increased concentrations of leaf mitochondrial-localized O2(-) and leaf NO in comparison to the Wt, while leaf concentrations of H(2)O(2) were similar or lower in the AOX-suppressed plants. • Based on our results, we suggest that AOX respiration acts to reduce the generation of ROS and RNS in plant mitochondria by dampening the leak of single electrons from the electron transport chain to O(2) or nitrite. This may represent a universal role for AOX in plants. More work is now needed to establish the functional implications of this role, such as during abiotic and biotic stress.
authors.affiliation: Department of Biological Sciences, University of Toronto Scarborough, Toronto, ON, Canada.
authors.forename: Marina
authors.initials: M
authors.lastname: Cvetkovska
authors.forename: Greg C
authors.initials: GC
authors.lastname: Vanlerberghe
mesh_terms: D006861:Hydrogen Peroxide / Q000378:metabolism; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D030421:Peroxynitrous Acid / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D000099188:Alternative Oxidase
chemical_list: D006861:Hydrogen Peroxide; D024101:Mitochondrial Proteins; D009569:Nitric Oxide; D010088:Oxidoreductases; D030421:Peroxynitrous Acid; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D013481:Superoxides; D000099188:Alternative Oxidase