title: Histopathological and postoperative behavioral comparison of rodent oral tongue resection: fiber-enabled CO2 laser versus electrocautery.
abstract: OBJECTIVE
  To compare operative time and hemostasis of fiber-enabled CO(2) laser (FECL) energy to that of the electrocautery (EC) technique for oral tongue resection, to compare return to oral intake and preoperative weight after FECL and EC resection, and to compare histologic changes in adjacent tissue after FECL and EC resection.
  
  
  METHODS
  Prospective animal study.
  
  
  METHODS
  Research laboratory.
  
  
  METHODS
  The CO(2) laser fiber and the Bovie cautery were each used to resect the anterior tongue in 15 adult rats. Fixative perfusion and killing were performed on postoperative day 0 (n = 10), 3 (n = 10), or 7 (n = 10). Body weight, food intake, and water intake were recorded daily for 3- and 7-day survival rats. After preparation for histologic analysis, the tongue tissue was graded with a mucosal wound-healing scale (MWHS).
  
  
  RESULTS
  A higher incidence of intraoperative bleeding and shorter operative times were noted in the EC group. No statistically significant difference in postoperative food or water intake between the EC and FECL groups was noted. The FECL group returned to baseline weight by postoperative day 6. MWHS scores were lower in the EC group by postoperative day 3 and lower in the FECL group by postoperative day 7.
  
  
  CONCLUSIONS
  Both EC and FECL are effective for resection of the tongue in rats. EC has the advantage of shorter operative time and lower MWHS scores by postoperative day 3; FECL has the advantages of less intraoperative bleeding, faster return to baseline body weight, and lower MWHS score by postoperative day 7.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA. cshires1@gmail.com
authors.forename: Courtney B
authors.initials: CB
authors.lastname: Shires
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Saputra
authors.forename: Lauren
authors.initials: L
authors.lastname: King
authors.forename: Jerome W
authors.initials: JW
authors.lastname: Thompson
authors.forename: Detlef H
authors.initials: DH
authors.lastname: Heck
authors.forename: Merry E
authors.initials: ME
authors.lastname: Sebelik
authors.forename: John D
authors.initials: JD
authors.lastname: Boughter
mesh_terms: D000818:Animals; D016063:Blood Loss, Surgical / Q000706:statistics & numerical data; D004195:Disease Models, Animal; D004564:Electrocoagulation / Q000379:methods*; D054020:Lasers, Gas / Q000627:therapeutic use*; D016014:Linear Models; D061646:Operative Time; D011446:Prospective Studies; D051381:Rats; D014059:Tongue / Q000601:surgery*; D015431:Weight Loss; D014945:Wound Healing