title: Breadth of cellular and humoral immune responses elicited in rhesus monkeys by multi-valent mosaic and consensus immunogens.
abstract: To create an HIV-1 vaccine that generates sufficient breadth of immune recognition to protect against the genetically diverse forms of the circulating virus, we have been exploring vaccines based on consensus and mosaic protein designs. Increasing the valency of a mosaic immunogen cocktail increases epitope coverage but with diminishing returns, as increasingly rare epitopes are incorporated into the mosaic proteins. In this study we compared the immunogenicity of 2-valent and 3-valent HIV-1 envelope mosaic immunogens in rhesus monkeys. Immunizations with the 3-valent mosaic immunogens resulted in a modest increase in the breadth of vaccine-elicited T lymphocyte responses compared to the 2-valent mosaic immunogens. However, the 3-valent mosaic immunogens elicited significantly higher neutralizing responses to Tier 1 viruses than the 2-valent mosaic immunogens. These findings underscore the potential utility of polyvalent mosaic immunogens for eliciting both cellular and humoral immune responses to HIV-1.
authors.affiliation: Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
authors.forename: Sampa
authors.initials: S
authors.lastname: Santra
authors.forename: Mark
authors.initials: M
authors.lastname: Muldoon
authors.forename: Sydeaka
authors.initials: S
authors.lastname: Watson
authors.forename: Adam
authors.initials: A
authors.lastname: Buzby
authors.forename: Harikrishnan
authors.initials: H
authors.lastname: Balachandran
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Carlson
authors.forename: Linh
authors.initials: L
authors.lastname: Mach
authors.forename: Wing-Pui
authors.initials: WP
authors.lastname: Kong
authors.forename: Krisha
authors.initials: K
authors.lastname: McKee
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Yang
authors.forename: Srinivas S
authors.initials: SS
authors.lastname: Rao
authors.forename: John R
authors.initials: JR
authors.lastname: Mascola
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Nabel
authors.forename: Bette T
authors.initials: BT
authors.lastname: Korber
authors.forename: Norman L
authors.initials: NL
authors.lastname: Letvin
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D004195:Disease Models, Animal; D000939:Epitopes / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D008253:Macaca mulatta; D054299:env Gene Products, Human Immunodeficiency Virus / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D000939:Epitopes; D054299:env Gene Products, Human Immunodeficiency Virus