title: Generation of human-induced pluripotent stem cells by a nonintegrating RNA Sendai virus vector in feeder-free or xeno-free conditions.
abstract: The generation of induced pluripotent stem cells (iPSCs) from somatic cells has enabled the possibility of providing unprecedented access to patient-specific iPSC cells for drug screening, disease modeling, and cell therapy applications. However, a major obstacle to the use of iPSC for therapeutic applications is the potential of genomic modifications caused by insertion of viral transgenes in the cellular genome. A second concern is that reprogramming often requires the use of animal feeder layers and reagents that contain animal origin products, which hinder the generation of clinical-grade iPSCs. Here, we report the generation of iPSCs by an RNA Sendai virus vector that does not integrate into the cells genome, providing transgene-free iPSC line. In addition, reprogramming can be performed in feeder-free condition with StemPro hESC SFM medium and in xeno-free (XF) conditions. Generation of an integrant-free iPSCs generated in xeno-free media should facilitate the safe downstream applications of iPSC-based cell therapies.
authors.affiliation: Primary and Stem Cell Systems, Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008, USA.
authors.forename: Chad C
authors.initials: CC
authors.lastname: Macarthur
authors.forename: Andrew
authors.initials: A
authors.lastname: Fontes
authors.forename: Namritha
authors.initials: N
authors.lastname: Ravinder
authors.forename: David
authors.initials: D
authors.lastname: Kuninger
authors.forename: Jasmeet
authors.initials: J
authors.lastname: Kaur
authors.forename: Matthew
authors.initials: M
authors.lastname: Bailey
authors.forename: Antje
authors.initials: A
authors.lastname: Taliana
authors.forename: Mohan C
authors.initials: MC
authors.lastname: Vemuri
authors.forename: Pauline T
authors.initials: PT
authors.lastname: Lieu