title: Silencing of fat-1 transgene expression in sheep may result from hypermethylation of its driven cytomegalovirus (CMV) promoter.
abstract: The fat-1 gene was isolated from roundworm Caenorhabditis elegans, and built into pIRES2-EGFP expression vectors driven by cytomegalovirus (CMV) promoter or cytomegalovirus enhancer and chickenβ-actin (CAG) promoter. Both CMV- and CAG-driven expression vectors were transfected to sheep fetal fibroblast cells. Positive transfected cells were used as donors for somatic cell nuclear transfer (SCNT) and the cloned embryos were transferred into the oviducts of synchronized recipient sheep. Two lambs derived from CMV vector and three lambs derived from CAG vector developed to term. Although Southern analyses using tissues from the two lambs derived from CMV vectors indicated integration of fat-1 gene into the genome, fat-1 mRNAs were not detected by RT-PCR. However, there was fat-1 expression (detected by RT-PCR) in tissues from transgenic lambs driven by CAG vectors. To investigate potential mechanisms involved in the two transgene models, methylation state of the vector promoters were examined. In CMV-driven transgenics, CMV promoters had almost no methylation in transfected cells and the resultant cloned embryos, whereas high methylations were detected in tissues and organs in transgenic lambs. In the CAG-driven transgenics, there were almost no methylations in transgenic cells and transgenic cloned embryos, and cloned lambs expressed fat-1 mRNA (detected by RT-PCR). Moreover, although SV40 promoters which drove neo/kan marker gene in CMV vectors were highly methylated in tissues from transgenic lambs, they were without methylation in cells and embryos. Therefore, we concluded that highly methylated CMV promoters induced the silence of fat-1 transgene expression in sheep. Furthermore, CAG promoter, but not CMV promoter was suitable for generation of fat-1 transgenic sheep.
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner mongolia University, Hohhot 010021, China.
authors.forename: B
authors.initials: B
authors.lastname: Duan
authors.forename: L
authors.initials: L
authors.lastname: Cheng
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: F X
authors.initials: FX
authors.lastname: Yin
authors.forename: G H
authors.initials: GH
authors.lastname: Su
authors.forename: Q Y
authors.initials: QY
authors.lastname: Shen
authors.forename: K
authors.initials: K
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Hu
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: G P
authors.initials: GP
authors.lastname: Li
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000831:Animals, Newborn; D017173:Caenorhabditis elegans / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary; D003587:Cytomegalovirus / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics / Q000502:physiology; D044943:Fatty Acid Desaturases / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing / Q000502:physiology*; D011247:Pregnancy; D011401:Promoter Regions, Genetic / Q000235:genetics; D012756:Sheep / Q000235:genetics*; D019076:Transgenes / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D029742:Caenorhabditis elegans Proteins; C466741:fat-1 protein, C elegans; D044943:Fatty Acid Desaturases