title: Determination of 17 macrolide antibiotics and avermectins residues in meat with accelerated solvent extraction by liquid chromatography-tandem mass spectrometry.
abstract: A method has been developed for simultaneous determination of 17 kinds of macrolide antibiotics and avermectins residues in animal origin foods. Samples were extracted with acetonitrile-methanol using accelerated solvent extraction (ASE) instrument. Parameters such as extraction temperature and pressure were investigated by a fractional factorial design (FFD) and the selected extraction (60 °C, 1500 psi for 10 min in two cycles) was most effective. High correlation coefficients (r > 0.999) of 17 macrolide antibiotics and avermectins were obtained within their respective linear ranges (2-400 μg/kg) using roxithromycin as internal standard. The recoveries of them were above 75% at different spiked levels in various samples. Using ASE the method was featured as short extraction times, reduction use of extraction solvent, high extraction yields, with high level of automation.
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues, HZAU/MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Tao
authors.forename: Gang
authors.initials: G
authors.lastname: Yu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Chen
authors.forename: Yuanhu
authors.initials: Y
authors.lastname: Pan
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Liu
authors.forename: Huimin
authors.initials: H
authors.lastname: Wei
authors.forename: Dapeng
authors.initials: D
authors.lastname: Peng
authors.forename: Lingli
authors.initials: L
authors.lastname: Huang
authors.forename: Yulian
authors.initials: Y
authors.lastname: Wang
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D059625:Liquid-Liquid Extraction / Q000379:methods*; D018942:Macrolides / Q000032:analysis* / Q000302:isolation & purification; D008460:Meat / Q000032:analysis*; D011312:Pressure; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D007559:Ivermectin; C019264:avermectin