title: In vivo administration of fluorescent dextrans for the specific and sensitive localization of brain vascular pericytes and their characterization in normal and neurotoxin exposed brains.
abstract: We have aimed to develop novel histochemical markers for the labeling of brain pericytes and characterize their morphology in the normal and the excitotoxin-exposed brain, as this class of cells has received little attention until recently. Pericyte labeling was accomplished by the intracerebroventricular injection of certain fluorescent dextran conjugates, such as Fluoro-Gold-dextran, FR-dextran, FITC-dextran and Fluoro-Turquoise (FT)-dextran. 1-7 days after the tracer injection, extensive labeling of vascular pericytes was seen throughout the entire brain. These cells were found distal to the endothelial cells and exhibited large dye containing vacuoles. The morphology of the pericytes was somewhat variable, exhibiting round or amoeboid shapes within larger intracellular vesicles, while those wrapping around capillaries exhibited a more elongated appearance with finger-like projections. The use of FG-dextran resulted in bluish yellow fluorescently labeled pericytes, while FR-dextran resulted in red fluorescent labeled pericytes, FITC-dextran exhibited green fluorescent pericytes and FT-dextran showed fluorescent blue pericytes in the brain. We have used these tracers to study possible changes in morphology and pericyte number following kainic acid insult, observing that the number of pericytes in the injured or lesioned areas of the brain is dramatically reduced compared to the non-injured areas. These novel fluorochromes should be of use for studies involving the detection and localization of pericytes in both normal and pathological brain tissues.
authors.affiliation: Division of Neurotoxicology, National Center for Toxicological Research/FDA, Jefferson, AR 72079, USA.
authors.forename: Sumit
authors.initials: S
authors.lastname: Sarkar
authors.forename: Larry
authors.initials: L
authors.lastname: Schmued
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D048430:Cell Shape / Q000187:drug effects; D003911:Dextrans / Q000008:administration & dosage* / Q000378:metabolism; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage / Q000633:toxicity*; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000031:analogs & derivatives; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007276:Injections, Intraventricular; D007608:Kainic Acid / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D008856:Microscopy, Fluorescence; D020286:Pericytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012235:Rhodamines / Q000008:administration & dosage; D013194:Staining and Labeling / Q000379:methods*; D013266:Stilbamidines / Q000008:administration & dosage; D013997:Time Factors
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D003911:Dextrans; D018690:Excitatory Amino Acid Agonists; D005456:Fluorescent Dyes; D012235:Rhodamines; D013266:Stilbamidines; C015219:fluorescein isothiocyanate dextran; C009434:tetramethylrhodamine isothiocyanate; D016650:Fluorescein-5-isothiocyanate; D007608:Kainic Acid