title: Interleukin-6 enhances porcine parthenote development in vitro, through the IL-6/Stat3 signaling pathway.
abstract: Signal transducer and activator of transcription-3 (Stat3) plays a central role in interleukin-6 (IL-6)-mediated cell proliferation by inhibiting apoptosis in a variety of cell types. The Stat3 pathway is essential for embryonic development. The aim of this study was to determine the effects of recombinant IL-6 on the viability and development of porcine diploid parthenotes cultured in vitro. Four-cell parthenotes, derived in vitro, were cultured to the blastocyst stage, with or without recombinant IL-6. The addition of 10 or 100 ng/ml of recombinant swine IL-6 into PZM3 medium increased the development rate of parthenotes to the blastocyst stage (P<0.05). When supplemented with 10 ng/ml of recombinant swine IL-6, the number of parthenotes at the blastocyst stage increased (P<0.05) and apoptosis decreased (P<0.05). Real-time RT-PCR experiments revealed that the addition of recombinant swine IL-6 decreased the mRNA expression of the pro-apoptotic gene Caspase3 (P<0.01) but increased the expression levels of the anti-apoptotic genes Bcl2l1 and Survivin. IL-6 receptors and Stat3 mRNA expression were upregulated after treatment with 10 ng/ml recombinant swine IL-6. Immunoblots and fluorescence labeling experiments showed that the levels of phosphorylated Stat3 were upregulated. These results suggest that recombinant swine IL-6 prevents apoptosis of porcine parthenotes and enhances porcine embryo viability through the IL-6/Stat3 signaling pathway in vitro.
authors.affiliation: Department of Histology and Embryology, Harbin Medical University, Heilongjiang Province, China.
authors.forename: Xing-Hui
authors.initials: XH
authors.lastname: Shen
authors.forename: Xiang-Shun
authors.initials: XS
authors.lastname: Cui
authors.forename: Sung-Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001755:Blastocyst / Q000502:physiology*; D054885:Cumulus Cells / Q000502:physiology; D004477:Ectogenesis; D005260:Female; D018507:Gene Expression Regulation, Developmental; D059471:In Vitro Oocyte Maturation Techniques; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000502:physiology*; D010312:Parthenogenesis; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism; D019947:Receptors, Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D034421:Sus scrofa
chemical_list: D051017:Apoptosis Regulatory Proteins; D015850:Interleukin-6; D012333:RNA, Messenger; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor