title: Approaches to measurement of vitamin D concentrations - mass spectrometry.
abstract: Mass spectrometry today is the only analytical technology which allows the specific determination of all known vitamin D metabolites. During the last few years the number of published methods rapidly increased and quantitative HPLC-MS/MS procedures are described for all the major metabolites including 25(OH)D3, 3-epi25(OH)D3, 24,25(OH)(2)D3, 25(OH)D2 and 1,25(OH)(2)D3. For the first time these new methods have made the systematic study of the clinical relevance of vitamin D metabolites possible. In parallel to the development of methods for new metabolites, significant progress was made in improving the performance of HPLC-MS/MS for quantifying 25(OH)D3 concentrations which has resulted in very short run times and thus enabling high throughput analysis for routine use and large sample sets.
authors.affiliation: Roche Diagnostic GmbH, Nonnenwald 2, D-82377 Penzberg - Germany. uwe.kobold@roche.com
authors.forename: Uwe
authors.initials: U
authors.lastname: Kobold
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007118:Immunoassay; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D014807:Vitamin D; C104450:25-hydroxyvitamin D