title: Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging.
abstract: The levels of the oxidised forms of guanosine in leukocytes, plasma and urine of Macaca mulatta were determined using a sensitive method based on high-performance liquid chromatography-triple quadruple mass spectrometry (LC-MS/MS). The amounts of 8-oxo-7,8-dihydrodeoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosin (8-oxoGsn), derived from DNA and RNA, respectively, increased with age in leukocytes. The measurement of the free forms of oxidised guanosine revealed similar age-dependent increases of 8-oxo-dGsn and 8-oxoGsn in both plasma and urine, which showed considerably larger amounts of 8-oxoGsn than 8-oxo-dGsn. The 8-oxoGsn content of urine could be a useful biomarker for evaluating aging, as age-dependent increases of 8-oxoGsn are more evident in urine compared to plasma and because urine samples are readily available.
authors.affiliation: Graduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing , PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shi
authors.forename: Ben
authors.initials: B
authors.lastname: Nie
authors.forename: Wei
authors.initials: W
authors.lastname: Gan
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Zhou
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Takagi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hayakawa
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Sekiguchi
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Cai
mesh_terms: D000375:Aging / Q000378:metabolism / Q000652:urine*; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism*; D005260:Female; D006151:Guanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D007962:Leukocytes / Q000737:chemistry / Q000378:metabolism*; D008253:Macaca mulatta; D018384:Oxidative Stress; D012313:RNA / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D006151:Guanosine; C046215:8-hydroxyguanosine; D012313:RNA; D004247:DNA