title: Argon-plasma-induced ultrathin thermal grafting of thermoresponsive pNIPAm coating for contractile patterned human SMC sheet engineering.
abstract: A new method for ultrathin grafting of pNIPAm on PDMS surfaces is introduced that employs plasma activation of the surface followed by thermal polymerization. This method is optimized for human primary SMC attachment and subsequent intact cell sheet detachment by lowering the temperature. The contractile gene expression of the cells showed that the contractile phenotype of the SMCs which is induced by aligning the cells through micropatterning is more preserved after thermoresponsive cell sheet detachment in contrast with enzymatic detachment. Given its simplicity and low cost, this thermoresponsive grafting method can be utilized for engineering patterned cell sheets for future bottom-up tissue engineering techniques.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore.
authors.forename: Shahrzad
authors.initials: S
authors.lastname: Rayatpisheh
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Mary B
authors.initials: MB
authors.lastname: Chan-Park
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001128:Argon; D015415:Biomarkers / Q000378:metabolism; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000494:pharmacology; D003285:Contractile Proteins / Q000235:genetics / Q000378:metabolism; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D015870:Gene Expression; D006801:Humans; D018625:Microscopy, Atomic Force; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D058626:Plasma Gases; D058105:Polymerization; D013499:Surface Properties; D013696:Temperature; D054457:Tissue Scaffolds
chemical_list: D000180:Acrylic Resins; D015415:Biomarkers; D020099:Coated Materials, Biocompatible; D003285:Contractile Proteins; D004129:Dimethylpolysiloxanes; D058626:Plasma Gases; C052970:poly-N-isopropylacrylamide; C013830:baysilon; D001128:Argon