title: NPSR1 gene is associated with reduced risk of rheumatoid arthritis.
abstract: OBJECTIVE
  Neuropeptide S receptor 1 (NPSR1) is a G protein-coupled receptor involved in immune response and is associated with several inflammatory diseases. We investigated the possible contribution of several polymorphisms in the intronic region of NPSR1 to rheumatoid arthritis (RA).
  
  
  METHODS
  Genotyping of 7 single-nucleotide polymorphisms (SNP) was performed in a total of 1232 patients with RA and 983 healthy controls of Spanish white origin by real-time polymerase chain reaction technology, using the TaqMan 5'-allele discrimination assay.
  
  
  RESULTS
  One out of the 7 SNP analyzed (rs740347) was associated with RA [p after Bonferroni correction (p(BNF)) = 1.2 × 10(-3), OR 0.73]. An association was also observed with rheumatoid factor-positive and shared epitope-positive RA (p(BNF) = 0.011, OR 0.73; p(BNF) = 0.037, OR 0.75, respectively).
  
  
  CONCLUSIONS
  Our results show that variations in the NPSR1 intronic region are associated with low risk in patients with RA, supporting other evidence that this locus represents a common genetic factor in inflammatory diseases.
authors.affiliation: Instituto de Parasitología y Biomedicina López-Neyra, CSIC, Granada, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: Robledo
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: González-Gay
authors.forename: Benjamín
authors.initials: B
authors.lastname: Fernández-Gutiérrez
authors.forename: José Ramón
authors.initials: JR
authors.lastname: Lamas
authors.forename: Alejandro
authors.initials: A
authors.lastname: Balsa
authors.forename: Dora
authors.initials: D
authors.lastname: Pascual-Salcedo
authors.forename: Santos
authors.initials: S
authors.lastname: Castañeda
authors.forename: Ricardo
authors.initials: R
authors.lastname: Blanco
authors.forename: Isidoro
authors.initials: I
authors.lastname: González-Alvaro
authors.forename: Antonio
authors.initials: A
authors.lastname: García
authors.forename: Enrique
authors.initials: E
authors.lastname: Raya
authors.forename: Carmen
authors.initials: C
authors.lastname: Gómez-Vaquero
authors.forename: Mario
authors.initials: M
authors.lastname: Delgado
authors.forename: Javier
authors.initials: J
authors.lastname: Martín
mesh_terms: D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D020641:Polymorphism, Single Nucleotide; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D012307:Risk Factors
chemical_list: C484532:NPSR1 protein, human; D043562:Receptors, G-Protein-Coupled