title: Molecular characterization of a catalase from Hydra vulgaris.
abstract: Catalase, an antioxidant and hydroperoxidase enzyme protects the cellular environment from harmful effects of hydrogen peroxide by facilitating its degradation to oxygen and water. Molecular information on a cnidarian catalase and/or peroxidase is, however, limited. In this work an apparent full length cDNA sequence coding for a catalase (HvCatalase) was isolated from Hydra vulgaris using 3'- and 5'- (RLM) RACE approaches. The 1859 bp HvCatalase cDNA included an open reading frame of 1518 bp encoding a putative protein of 505 amino acids with a predicted molecular mass of 57.44 kDa. The deduced amino acid sequence of HvCatalase contained several highly conserved motifs including the heme-ligand signature sequence RLFSYGDTH and the active site signature FXRERIPERVVHAKGXGA. A comparative analysis showed the presence of conserved catalytic amino acids [His(71), Asn(145), and Tyr(354)] in HvCatalase as well. Homology modeling indicated the presence of the conserved features of mammalian catalase fold. Hydrae exposed to thermal, starvation, metal and oxidative stress responded by regulating its catalase mRNA transcription. These results indicated that the HvCatalase gene is involved in the cellular stress response and (anti)oxidative processes triggered by stressor and contaminant exposure.
authors.affiliation: Faculty of Toxicology, Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Bhagirathi
authors.initials: B
authors.lastname: Dash
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Phillips
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002374:Catalase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D006358:Hot Temperature; D006829:Hydra / Q000201:enzymology* / Q000235:genetics; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D018384:Oxidative Stress / Q000502:physiology; D016415:Sequence Alignment; D013217:Starvation / Q000378:metabolism
chemical_list: D008670:Metals; D002374:Catalase