title: Establishment of a monoclonal anti-pan HLA class I antibody suitable for immunostaining of formalin-fixed tissue: unusually high frequency of down-regulation in breast cancer tissues.
abstract: A novel monoclonal anti-pan human leukocyte antigen (HLA) class I heavy chain antibody, EMR8-5, was established. It could detect HLA-A, -B, and -C antigens in formalin-fixed paraffin embedded tissues. By immunohistochemical staining using the EMR8-5 antibody, various cancer tissues from 246 cases were examined for HLA class I expression. It was found that HLA class I expression was decreased in 20% to 42% of the cases of lung cancer, hepatocellular carcinoma, colon cancer, renal cell carcinoma, and urothelial carcinoma. In contrast, 85% of breast cancer cases had loss of or decreased HLA class I expression. Of the 35 breast cancer cases that had decreased HLA class I heavy chain expression, 33 (94%) also had decreased beta2-microglobulin expression detected by immunohistochemical staining. It was suggested that HLA class I down-regulation might be a common characteristic of breast cancer mostly caused by the down-regulation of beta2-microglobulin expression.
authors.affiliation: Departments of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan. torigoe@sapmed.ac.jp
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Torigoe
authors.forename: Hiroko
authors.initials: H
authors.lastname: Asanuma
authors.forename: Emiri
authors.initials: E
authors.lastname: Nakazawa
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Tamura
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hirohashi
authors.forename: Eri
authors.initials: E
authors.lastname: Yamamoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kanaseki
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D005557:Formaldehyde; D005805:Genes, MHC Class I / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016707:Tissue Fixation; D001613:beta 2-Microglobulin / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015395:Histocompatibility Antigens Class I; D001613:beta 2-Microglobulin; D005557:Formaldehyde