title: Molecular cloning, tissue distribution, and functional analysis of porcine Akirin2.
abstract: Akirin2 is a recently discovered gene that is involved in innate immune response. In this study, the porcine Akirin2 gene was cloned. The full-length coding sequence (CDS) of porcine Akirin2 consists of 612 bp and encodes 203 amino acids with a molecular mass of 22493 kD. The homology tree analysis showed that the pig Akirin2 has closer genetic relationships and distance with the known mammalian Akirin2. Real time quantitative PCR analysis showed that the porcine Akirin2 transcript was most abundant in the lung, followed by the skeletal muscle, heart, liver, fat, thymus, lymph node, small intestine, kidney, and spleen. Overexpression of porcine Akirin2 increased expression of IL-6 in porcine jejunal epithelial cell line IPEC-J2 cells. Our data suggest that porcine Akirin2 could play an important role in intestinal immune regulation.
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, P. R. China. xlchen@sicau.edu.cn
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
authors.forename: Gang
authors.initials: G
authors.lastname: Jia
authors.forename: Xiuqun
authors.initials: X
authors.lastname: Wu
authors.forename: Caimei
authors.initials: C
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016415:Sequence Alignment; D013552:Swine
chemical_list: C559423:Akirin2 protein, mouse; D015850:Interleukin-6; D012333:RNA, Messenger; D012097:Repressor Proteins