title: A circular dichroism study uncovers a two-step interaction of antitumor azolato-bridged dinuclear platinum(II) complexes with calf thymus DNA.
abstract: The interactions of four antitumor azolato-bridged dinuclear platinum(II) complexes, [{cis-Pt(NH(3))(2)}(2)(μ-OH)(μ-azolato)](2+), with calf thymus DNA were monitored dose- and time-dependently, by using circular dichroism. Complexes 1-4 reacted with DNA via a two-step interaction that comprised a prompt diffusion-controlled reaction, which induced a B- to C-form transition, and a relatively slow temperature-dependent reaction.
authors.affiliation: Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka, Mie 513-8670, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Uemura
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Chikuma
authors.forename: Seiji
authors.initials: S
authors.lastname: Komeda
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002942:Circular Dichroism / Q000379:methods*; D002945:Cisplatin / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D019281:Dimerization; D010984:Platinum / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D010984:Platinum; D004247:DNA; C427203:calf thymus DNA; D002945:Cisplatin