title: Transduced Tat-DJ-1 protein protects against oxidative stress-induced SH-SY5Y cell death and Parkinson disease in a mouse model.
abstract: Parkinson's disease (PD) is a well known neurodegenerative disorder characterized by selective loss of dopaminergic neurons in the substantia nigra pars compact (SN). Although the exact mechanism remains unclear, oxidative stress plays a critical role in the pathogenesis of PD. DJ-1 is a multifunctional protein, a potent antioxidant and chaperone, the loss of function of which is linked to the autosomal recessive early onset of PD. Therefore, we investigated the protective effects of DJ-1 protein against SH-SY5Y cells and in a PD mouse model using a cell permeable Tat-DJ-1 protein. Tat-DJ-1 protein rapidly transduced into the cells and showed a protective effect on 6-hydroxydopamine (6-OHDA)-induced neuronal cell death by reducing the reactive oxygen species (ROS). In addition, we found that Tat-DJ-1 protein protects against dopaminergic neuronal cell death in 1-methyl-4-phenyl-1,2,3,6,-tetrahydropyridine (MPTP)-induced PD mouse models. These results suggest that Tat-DJ-1 protein provides a potential therapeutic strategy for against ROS related human diseases including PD.
authors.affiliation: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Korea.
authors.forename: Hoon Jae
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Su Jung
authors.initials: SJ
authors.lastname: Woo
authors.forename: Hye Ri
authors.initials: HR
authors.lastname: Kim
authors.forename: So Mi
authors.initials: SM
authors.lastname: Kim
authors.forename: Hyo Sang
authors.initials: HS
authors.lastname: Jo
authors.forename: Meeyoung
authors.initials: M
authors.lastname: Park
authors.forename: Duk-Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Oh-Shin
authors.initials: OS
authors.lastname: Kwon
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Kyu Hyung
authors.initials: KH
authors.lastname: Han
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D016923:Cell Death / Q000235:genetics / Q000502:physiology; D059290:Dopaminergic Neurons / Q000378:metabolism / Q000502:physiology; D015696:Gene Products, tat / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018696:Neuroprotective Agents / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D010300:Parkinson Disease / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D054464:Peroxiredoxins; D000071617:Protein Deglycase DJ-1; D017382:Reactive Oxygen Species / Q000378:metabolism; D013378:Substantia Nigra / Q000378:metabolism / Q000502:physiology; D014161:Transduction, Genetic / Q000379:methods
chemical_list: D000975:Antioxidants; D015696:Gene Products, tat; D018696:Neuroprotective Agents; D015513:Oncogene Proteins; D017382:Reactive Oxygen Species; D054464:Peroxiredoxins; C503466:PARK7 protein, mouse; D000071617:Protein Deglycase DJ-1