title: Formation of engineered bone with adipose stromal cells from buccal fat pad.
abstract: A robust method for inducing bone formation from adipose-derived stromal cells (ADSCs) has not been established. Moreover, the efficacy of strong osteogenic inducers including BMP-2 for ADSC-mediated bone engineering remains controversial. Meanwhile, the buccal fat pad (BFP), which is found in the oral cavity as an adipose-encapsulated mass, has been shown to have potential as a new accessible source of ADSCs for oral surgeons. However, to date, there have been no reports that define the practical usefulness of ADSCs from BFP (B-ADSCs) for bone engineering. Here, we report an efficient method of generating bone from B-ADSCs using rhBMP-2. The analyses show that B-ADSCs can differentiate in vitro toward the osteoblastic lineage by the addition of rhBMP-2 to culture medium, regardless of the presence of osteoinductive reagents (OSR), as demonstrated by measurements of ALP activity, in vitro calcification, and osteogenic gene expression. Interestingly, adipogenic genes were clearly detectable only in cultures with rhBMP-2 and OSR. However, in vivo bone formation was most substantial when B-ADSCs cultured in this condition were transplanted. Thus, B-ADSCs reliably formed engineered bone when pre-treated with rhBMP-2 for inducing mature osteoblastic differentiation. This study supports the potential translation for B-ADSC use in the clinical treatment of bone defects.
authors.affiliation: Department of Regenerative Oral Surgery, Unit of Translational Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shiraishi
authors.forename: Y
authors.initials: Y
authors.lastname: Sumita
authors.forename: Y
authors.initials: Y
authors.lastname: Wakamastu
authors.forename: K
authors.initials: K
authors.lastname: Nagai
authors.forename: I
authors.initials: I
authors.lastname: Asahina
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000293:Adolescent; D000328:Adult; D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D001861:Bone Regeneration; D002113:Calcification, Physiologic; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002610:Cheek; D017077:Culture Media, Conditioned; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010006:Osteoblasts / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D017154:Stromal Cells / Q000187:drug effects* / Q000637:transplantation*; D023822:Tissue Engineering / Q000379:methods*; D014746:Vimentin / Q000096:biosynthesis; D055815:Young Adult
chemical_list: D000954:Antigens, Surface; D055396:Bone Morphogenetic Protein 2; D017077:Culture Media, Conditioned; D011994:Recombinant Proteins; C525591:STRO-1 antigen, human; D014746:Vimentin