title: Inhibition of 6-hydroxydopamine-induced endoplasmic reticulum stress             by sulforaphane through the activation of Nrf2 nuclear translocation.
abstract: Endoplasmic reticulum (ER) stress plays a key role in the development of             neurodegenerative diseases, including Parkinson's disease (PD). Sulforaphane (SF)             is a natural drug derived from isothiocyanate found in cruciferous vegetables.             Although there are reports indicating that SF is a potential candidate for PD             treatment, there have been no reports on the effects of SF on ER stress in PD.             In this study, we investigated the cytoprotective effects of SF on 6-hydroxydopamine             (6-OHDA)-induced ER stress in rat PC12 cells. Pre-treatment with SF elicited cytoprotection             against 6-OHDA-induced cytotoxicity. Consistent with its cytoprotective action,             SF significantly inhibited subsequent ER stress, including the expression of Bip             and the C/EBP homologous protein. We also found that transfection with NF-E2-related             factor-2 (Nrf2) siRNA reversed the inhibitory effects of SF on 6-OHDA-induced             ER stress responses. In conclusion, our results show that SF can prevent ER stress             response induced by 6-OHDA through the activation of Nrf2. SF may be a therapeutic             candidate for the treatment of ER stress-associated neural diseases, including             PD.
authors.affiliation: Deparment of Emergency, the People's Hospital of Guang Du District, Kunming 650200, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Deng
authors.forename: Ran
authors.initials: R
authors.lastname: Tao
authors.forename: Shun-Zhi
authors.initials: SZ
authors.lastname: Yu
authors.forename: Hua
authors.initials: H
authors.lastname: Jin
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D015870:Gene Expression; D020868:Gene Silencing; D017879:Isothiocyanates; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D016627:Oxidopamine / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D016716:PC12 Cells; D021381:Protein Transport; D051381:Rats; D013454:Sulfoxides; D013861:Thiocyanates / Q000494:pharmacology*
chemical_list: D017879:Isothiocyanates; D051267:NF-E2-Related Factor 2; D013454:Sulfoxides; D013861:Thiocyanates; D016627:Oxidopamine; C016766:sulforaphane