title: Effectivity of freeze-dried form of Lactobacillus fermentum AD1-CCM7421 in dogs.
abstract: The impact of probiotic supplementation of canine-derived strain Lactobacillus fermentum AD1-CCM7421 in freeze-dried form on quantitative composition of microbiota and short-chain fatty acid profile in feces of dogs was demonstrated by two independent studies (straightforward repeated-measures model; study I: a dose of 2 g per dog for 2 weeks, 10(8) CFU/g, n = 12; study II: 1 g per dog for 1 week, 10(7) CFU/g, n = 11. The results revealed a significant increase of lactic acid bacteria population persisting also after the cessation of probiotic application in both studies. A reduction of clostridia (study I, p                         (sum) < 0.01) and tested Gram-negative bacterial genera (coliforms, Aeromonas sp., Pseudomonas sp., study II, p < 0.05) was also detected. The strain AD1-CCM7421 colonized the canine digestive tract in sufficient numbers (10(5)-10(6) CFU/g) and it persisted in the majority of dogs after cessation of probiotic application. An increase of short-chain fatty acid concentrations (study I: butyric, succinic, valeric, formic acid) especially in the early post-treatment phase (p < 0.05) most likely led to a decrease of fecal pH value (p < 0.05) without negative influence on fecal consistency throughout the studies.
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia. strompfv@saske.sk
authors.forename: V
authors.initials: V
authors.lastname: Strompfová
authors.forename: A
authors.initials: A
authors.lastname: Lauková
authors.forename: S
authors.initials: S
authors.lastname: Gancarčíková
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D019587:Dietary Supplements / Q000032:analysis; D004283:Dog Diseases / Q000378:metabolism / Q000382:microbiology / Q000517:prevention & control*; D004285:Dogs; D005227:Fatty Acids / Q000378:metabolism; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D005612:Freeze Drying; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology; D052197:Limosilactobacillus fermentum / Q000737:chemistry / Q000502:physiology*; D054892:Metagenome; D019936:Probiotics / Q000737:chemistry*
chemical_list: D005227:Fatty Acids