title: PRORP proteins support RNase P activity in both organelles and the nucleus in Arabidopsis.
abstract: RNase P is an essential enzyme that cleaves the 5' leader sequence of tRNA precursors. RNase Ps were believed until now to occur universally as ribonucleoproteins in organisms performing RNase P activity. Here we find that protein-only RNase P enzymes called PRORP (for proteinaceous RNase P) support RNase P activity in vivo in both organelles and the nucleus in Arabidopsis. Beyond tRNA, PRORP proteins are involved in the maturation of small nucleolar RNA (snoRNA) and mRNA. Finally, ribonucleoprotein RNase MRP is not involved in tRNA maturation in plants. Altogether, our results indicate that ribonucleoprotein enzymes have been entirely replaced by proteins for RNase P activity in plants.
authors.affiliation: Institut de Biologie moléculaire des plantes du CNRS, University of Strasbourg, 67084 Strasbourg, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Gutmann
authors.forename: Anthony
authors.initials: A
authors.lastname: Gobert
authors.forename: Philippe
authors.initials: P
authors.lastname: Giegé
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D020537:RNA, Small Nucleolar / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D043262:Ribonuclease P / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D012333:RNA, Messenger; D020537:RNA, Small Nucleolar; D012261:Ribonucleoproteins; D012343:RNA, Transfer; D043262:Ribonuclease P