title: Osteopontin increases the proliferation of neural progenitor cells.
abstract: We examined the role of osteopontin in the proliferation of neural progenitor cells in vitro. Osteopontin increased the proliferation of neural progenitor cells in the presence of FGF2 as measured by cell proliferation assay and bromodeoxy uridine incorporation studies. In addition, immunoblot analysis demonstrated an increase in the phosphorylation of retinoblastoma protein with a concurrent increase in the content of phospho-Akt and cyclin D1. These results indicate that osteopontin can upregulate the content of phospho-Akt, cyclin D1 and phospho-Rb to subsequently enhance the proliferation of neural progenitor cells in the presence of FGF2.
authors.affiliation: Department of Neurosurgery, University of Wisconsin, Madison, WI 53792, United States. h.kalluri@neurosurg.wisc.edu
authors.forename: Haviryaji S G
authors.initials: HS
authors.lastname: Kalluri
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Dempsey
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D001973:Bromodeoxyuridine / Q000378:metabolism; D016203:CDC2 Protein Kinase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D058953:Neural Stem Cells / Q000187:drug effects*; D053495:Osteopontin / Q000494:pharmacology*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D016160:Retinoblastoma Protein / Q000378:metabolism
chemical_list: D016160:Retinoblastoma Protein; D016222:Fibroblast Growth Factor 2; D053495:Osteopontin; D051057:Proto-Oncogene Proteins c-akt; D016203:CDC2 Protein Kinase; D001973:Bromodeoxyuridine