title: Lobe-specific heterogeneity and matrix metalloproteinase activation after ischemia/reperfusion injury in rat livers.
abstract: Studies assessing the effects of partial-hepatic ischemia/reperfusion (I/R) injury focused on the damage to the ischemic-lobe, whereas few data are available on non-ischemic lobe. This study investigated whether acute liver I/R does affect non-ischemic lobe function via modulation of extracellular matrix remodeling. Male Sprague-Dawley rats underwent left lateral- and median-lobe ischemia for 30 min and reperfusion for 60 min or sham operation. After reperfusion, blood samples and hepatic biopsies from both the ischemic (left-lobe, LL) and the non-ischemic lobe (right-lobe, RL) were collected. Serum hepatic enzymes and TNF-alpha, tissue matrix metalloproteinases (MMP-2, MMP-9), liver morphology, malondialdehyde (MDA), and myeloperoxidase (MPO) were evaluated. Liver I/R injury was confirmed by altered increased hepatic enzymes and TNF-alpha. I/R induced an altered morphology and an increase in MMP-2 and MMP-9 activity not only in left-ischemic lobe (LL) but also in the right-non-ischemic (RL) lobe. A lobar difference was detected for MDA formation and MPO activity in both sham and I/R submitted rats, with higher levels in the left lobe for both groups. This study indicates that an increase in MMPs, which may be TNF-alpha-mediated, occurs in both the ischemic- and the non-ischemic lobes; the heterogeneous lobe concentrations of MDA and MPO suggest that the random sampling of liver tissue should be avoided.
authors.affiliation: Department of Internal Medicine and Therapeutics, University of Pavia, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Palladini
authors.forename: Andrea
authors.initials: A
authors.lastname: Ferrigno
authors.forename: Vittoria
authors.initials: V
authors.lastname: Rizzo
authors.forename: Eleonora
authors.initials: E
authors.lastname: Boncompagni
authors.forename: Plinio
authors.initials: P
authors.lastname: Richelmi
authors.forename: Isabel
authors.initials: I
authors.lastname: Freitas
authors.forename: Stefano
authors.initials: S
authors.lastname: Perlini
authors.forename: Mariapia
authors.initials: M
authors.lastname: Vairetti
mesh_terms: D000818:Animals; D005109:Extracellular Matrix / Q000378:metabolism; D007511:Ischemia / Q000473:pathology; D008099:Liver / Q000201:enzymology / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D020778:Matrix Metalloproteinase 2 / Q000097:blood*; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D009195:Peroxidase / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D015854:Up-Regulation
chemical_list: D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D009195:Peroxidase; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat