title: A 50-kilodalton Cry2A peptide is lethal to Bombyx mori and Lymantria dispar.
abstract: The Cry2Aa3 gene was introduced into asporogenic Bacillus thuringiensis, and the synthesized protoxin killed Bombyx mori and Lymantria dispar larvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Val50 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides killed the larvae of both insects.
authors.affiliation: Laboratory of Applied Biochemistry, Graduate School of Science and Technology, Niigata University, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Ohsawa
authors.forename: Miki
authors.initials: M
authors.lastname: Tanaka
authors.forename: Kenta
authors.initials: K
authors.lastname: Moriyama
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Shimazu
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Asano
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Haginoya
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Mitsui
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Kouya
authors.forename: Masayuki
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Hori
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism / Q000633:toxicity*; D002918:Chymotrypsin / Q000378:metabolism; D004731:Endotoxins / Q000378:metabolism / Q000633:toxicity*; D006460:Hemolysin Proteins / Q000378:metabolism / Q000633:toxicity*; D006868:Hydrolysis; D007915:Lepidoptera / Q000187:drug effects* / Q000502:physiology*; D010455:Peptides / Q000633:toxicity; D016019:Survival Analysis
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D010455:Peptides; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D002918:Chymotrypsin