title: Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24.
abstract: Rhodococcus sp. CPZ24 was isolated from swine wastewater and identified. Batch (0.25 L flask) experiments of nitrogen removal under aerobic growth conditions showed complete removal of 50 mg L(-1) ammonium nitrogen within 20 h, while nitrate nitrogen removal reached 67%. A bioreactor (50 L) was used to further assess the heterotrophic nitrification and aerobic denitrification abilities of Rhodococcus sp. CPZ24. The results showed that 85% of the ammonium nitrogen (100 mg L(-1)) was transformed to nitrification products (NO(3)(-)-N and NO(2)(-)-N) (13%), intracellular nitrogen (24%), and gaseous denitrification products (48%) within 25 h. The ammonium nitrogen removal rate was 3.4 mg L(-1)h(-1). The results indicate that the strain CPZ24 carries out simultaneous nitrification and denitrification, demonstrating a potential use of the strain for wastewater treatment.
authors.affiliation: College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Peizhen
authors.initials: P
authors.lastname: Chen
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.forename: Qing X
authors.initials: QX
authors.lastname: Li
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Wang
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Li
authors.forename: Tianzhi
authors.initials: T
authors.lastname: Ren
authors.forename: Ligang
authors.initials: L
authors.lastname: Wang
mesh_terms: D000332:Aerobiosis; D000641:Ammonia / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D004275:DNA, Ribosomal / Q000235:genetics; D058440:Denitrification / Q000502:physiology*; D052836:Heterotrophic Processes / Q000502:physiology*; D008969:Molecular Sequence Data; D009566:Nitrates / Q000302:isolation & purification; D058465:Nitrification / Q000502:physiology*; D010084:Oxidation-Reduction; D010802:Phylogeny; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012240:Rhodococcus / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D004275:DNA, Ribosomal; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D000641:Ammonia