title: Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides.
abstract: Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, 4072, Australia.
authors.forename: Camille
authors.initials: C
authors.lastname: Dubois
authors.forename: Meghan A
authors.initials: MA
authors.lastname: Campbell
authors.forename: Stacey L
authors.initials: SL
authors.lastname: Edwards
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
authors.forename: Rakesh N
authors.initials: RN
authors.lastname: Veedu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods; D013379:Substrate Specificity; D019712:Thermococcus / Q000737:chemistry / Q000201:enzymology*
chemical_list: D052157:Aptamers, Nucleotide; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase