title: Malachite green derivatives for two-photon RNA detection.
abstract: The design, preparation and characterisation of a library of malachite green (MG) derivatives for two-photon RNA labelling is described. Some of these MG derivatives exhibit an increased affinity for an MG-aptamer, as well as improved two-photon sensitivity when compared to the classical malachite green chloride. The underlying mechanisms and potential benefits for in vivo RNA visualisation are discussed.
authors.affiliation: Laboratoire de Biophotonique et Pharmacologie, UMR 7213 UdS/CNRS, 74 route du Rhin, 67401 Illkirch, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Lux
authors.forename: Eduardo José
authors.initials: EJ
authors.lastname: Peña
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bolze
authors.forename: Manfred
authors.initials: M
authors.lastname: Heinlein
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Nicoud
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D017785:Photons; D012313:RNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D012394:Rosaniline Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D012394:Rosaniline Dyes; C005095:malachite green; D012313:RNA