title: Comparison of alpha-factor preprosequence and a classical mammalian signal peptide for secretion of recombinant xylanase xynB from yeast Pichia pastoris.
abstract: The secretory efficiency of recombinant xylanase xynB from yeast Pichia pastoris between the alpha-factor preprosequence and a classical mammalian signal peptide derived from bovine beta-casein was compared. The results showed that although the bovine beta-casein signal peptide could direct highlevel secretion of recombinant xylanase, it was relatively less efficient than the alpha-factor preprosequence. In contrast, the bovine beta-casein signal peptide caused remarkably more recombinant xylanase trapped intracellularly. Realtime RT-PCR analysis indicated that the difference in the secretory level between the two signal sequences was not due to the difference in the transcriptional efficiency.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, People's Republic of China.
authors.forename: Zuyong
authors.initials: Z
authors.lastname: He
authors.forename: Yuankai
authors.initials: Y
authors.lastname: Huang
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Qin
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Liu
authors.forename: Delin
authors.initials: D
authors.lastname: Mo
authors.forename: Peiqing
authors.initials: P
authors.lastname: Cong
authors.forename: Yaosheng
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002364:Caseins / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D000072235:Mating Factor; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics*; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D015202:Protein Engineering; D021382:Protein Sorting Signals; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*; D001617:beta-Glucosidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002364:Caseins; D010455:Peptides; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D000072235:Mating Factor; D001617:beta-Glucosidase; D043364:Endo-1,4-beta Xylanases; C090001:XynB xylanase