title: Relationship between thresholds to convulsions induced by a benzodiazepine inverse agonist and [³H]-L-glutamate binding in the membranes of brain regions.
abstract: Although some studies have investigated the influence of kindling model of epilepsy on the glutamatergic neurotransmission, the relation between glutamatergic receptors and seizure susceptibility remains unclear. The present study sought to determine if rats with high (HTR) and low (LTR) thresholds to clonic convulsions induced by the benzodiazepine inverse agonist DMCM differed in the [(3)H]-L-glutamate binding to membranes from discrete brain regions. Compared to the HTR subgroup, the LTR subgroup presented a lower binding of [(3)H]-L-glutamate in the hippocampus, frontal cortex and amygdala plus limbic cortex, suggesting that glutamatergic receptors in these brain regions may underlie the susceptibility to DMCM-induced convulsions.
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, Escola Paulista de Medicina (UNIFESP/EPM), Rua Botucatu 862, 1° andar, Vila Clementino, São Paulo, São Paulo 04023-062, Brazil. mmmarcos@uol.com.br
authors.forename: Marcos Brandão
authors.initials: MB
authors.lastname: Contó
authors.forename: José Gilberto Barbosa
authors.initials: JG
authors.lastname: de Carvalho
authors.forename: Marco Antonio Campana
authors.initials: MA
authors.lastname: Venditti
mesh_terms: D000818:Animals; D001569:Benzodiazepines / Q000633:toxicity*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002243:Carbolines / Q000494:pharmacology; D003292:Convulsants / Q000633:toxicity*; D004195:Disease Models, Animal; D018698:Glutamic Acid / Q000493:pharmacokinetics*; D008297:Male; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced* / Q000473:pathology*; D014316:Tritium / Q000493:pharmacokinetics
chemical_list: D002243:Carbolines; D003292:Convulsants; D014316:Tritium; D001569:Benzodiazepines; C034818:methyl 6,7-dimethoxy-4-ethyl-beta-carboline-3-carboxylate; D018698:Glutamic Acid