title: Hydrogen sulfide prevents formaldehyde-induced neurotoxicity to PC12 cells by attenuation of mitochondrial dysfunction and pro-apoptotic potential.
abstract: Hydrogen sulfide (H(2)S) has been shown to act as a neuroprotectant and antioxidant. Numerous studies have demonstrated that exposure to formaldehyde (FA) causes neuronal damage and that oxidative stress is one of the most critical effects of FA exposure. Accumulation of FA is involved in the pathogenesis of Alzheimer's disease (AD). The aim of present study is to explore the inhibitory effects of H(2)S on FA-induced cytotoxicity and apoptosis and the molecular mechanisms underlying in PC12 cells. We show that sodium hydrosulfide (NaHS), a H(2)S donor, protects PC12 cells against FA-mediated cytotoxicity and apoptosis and that NaHS preserves the function of mitochondria by preventing FA-induced loss of mitochondrial membrane potential and release of cytochrome c in PC12 cells. Furthermore, NaHS blocks FA-exerted accumulation of intracellular reactive oxygen species (ROS), down-regulation of Bcl-2 expression, and up-regulation of Bax expression. These results indicate that H(2)S protects neuronal cells against neurotoxicity of FA by preserving mitochondrial function through attenuation of ROS accumulation, up-regulation of Bcl-2 level, and down-regulation of Bax expression. Our study suggests a promising future of H(2)S-based preventions and therapies for neuronal damage after FA exposure.
authors.affiliation: Key Laboratory for Arteriosclerology of Hunan Province, University of South China, Hengyang 421001, Hunan, PR China. txq01001@gmail.com
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Tang
authors.forename: Yan-Kai
authors.initials: YK
authors.lastname: Ren
authors.forename: Cheng-Fang
authors.initials: CF
authors.lastname: Zhou
authors.forename: Chun-Tao
authors.initials: CT
authors.lastname: Yang
authors.forename: Hong-Feng
authors.initials: HF
authors.lastname: Gu
authors.forename: Jian-Qin
authors.initials: JQ
authors.lastname: He
authors.forename: Rong-Qian
authors.initials: RQ
authors.lastname: Chen
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhuang
authors.forename: Heng-Rong
authors.initials: HR
authors.lastname: Fang
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D004789:Enzyme Activation; D005434:Flow Cytometry; D005557:Formaldehyde / Q000633:toxicity*; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D005557:Formaldehyde; D045304:Cytochromes c; D053148:Caspase 3; D006862:Hydrogen Sulfide