title: Optimal timing for oocyte denudation and intracytoplasmic sperm injection.
abstract: Objectives. To analyze the impact of oocyte denudation and microinjection timings on intracytoplasmic sperm injection (ICSI) outcomes. Study Design. We included ICSI cycles with the following parameters: rank 1 or 2, female age <36 years, male factor infertility, long protocol using GnRH agonist and rFSH for ovarian stimulation, and use of freshly ejaculated sperm (n = 110). Several ICSI parameters were analyzed according to the time between oocyte retrieval and denudation (T(1)) and the time between denudation and ICSI (T(2)) using a statistical logistic regression analysis. Results. Neither T(1) nor T(2) had a significant influence on the Metaphase II (MII) rate but the fertilisation rate (FR) showed a significant improvement when T(1) was longer (optimal results at T(1) = 3 hours) while FR significantly decreased with the increase of T(2). Optimal implantation (IR) and pregnancy (PR) rates were obtained when T(1) was around 2 hours. Conclusion. Incubation of oocytes around 2 hours between retrieval and denudation may not increase MII rate but appears to lead to the optimal combination of FR and IR.
authors.affiliation: Laboratoire de Biologie de la Reproduction, AP-HP, Hôpital Bichat Université Paris Diderot, 46 Rue Henri Huchard, 75018 Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Patrat
authors.forename: Aida
authors.initials: A
authors.lastname: Kaffel
authors.forename: Lucie
authors.initials: L
authors.lastname: Delaroche
authors.forename: Juliette
authors.initials: J
authors.lastname: Guibert
authors.forename: Pierre
authors.initials: P
authors.lastname: Jouannet
authors.forename: Sylvie
authors.initials: S
authors.lastname: Epelboin
authors.forename: Dominique
authors.initials: D
authors.lastname: De Ziegler
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Wolf
authors.forename: Patricia
authors.initials: P
authors.lastname: Fauque