title: A peptide fraction from germinated soybean protein down-regulates PTTG1 and TOP2A mRNA expression, inducing apoptosis in cervical cancer cells.
abstract: The aim of this study was to evaluate the effect of a peptide fraction, obtained from a germinated soybean protein hydrolysate, on the viability, apoptosis and cancer related gene expression in HeLa cells. Soybean was germinated for 0-6 days and proteins were isolated from the seeds. Protein isolates, without ethanol-soluble phytochemicals, were hydrolyzed with digestive enzymes and their effect on growth in HeLa cells was evaluated. The most active hydrolysate was separated by ultrafiltration into five peptide fractions. A >10 kDa fraction was the most active against cancer cells. This fraction down-regulated PTTG1 and TOP2A mRNA expression (two genes considered as therapeutic targets) and induced apoptosis in cancer cells activating the caspase cascade and causing DNA fragmentation. Germinated soy protein isolates could be a bioactive ingredient of functional food.
authors.affiliation: Departamento de Graduados en Alimentos, Escuela Nacional de Ciencias Biologicas, I.P.N. Carpio y Plan de Ayala, Col. Sto.Tomas, 11340, Mexico, D.F.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Robles-Ramírez
authors.forename: Eva
authors.initials: E
authors.lastname: Ramón-Gallegos
authors.forename: Rosalva
authors.initials: R
authors.lastname: Mora-Escobedo
authors.forename: Nimbe
authors.initials: N
authors.lastname: Torres-Torres
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D004250:DNA Topoisomerases, Type II / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D018525:Germination; D006367:HeLa Cells; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D000075223:Poly-ADP-Ribose Binding Proteins; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D064249:Securin; D030262:Soybean Proteins / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*
chemical_list: D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D000075223:Poly-ADP-Ribose Binding Proteins; D012333:RNA, Messenger; D064249:Securin; D030262:Soybean Proteins; C578002:pituitary tumor-transforming protein 1, human; D004250:DNA Topoisomerases, Type II; C000618811:TOP2A protein, human