title: Rodent motor and neuropsychological behaviour measured in home cages using the integrated modular platform SmartCage™.
abstract: 1. To facilitate investigation of diverse rodent behaviours in rodents' home cages, we have developed an integrated modular platform, the SmartCage(™) system (AfaSci, Inc. Burlingame, CA, USA), which enables automated neurobehavioural phenotypic analysis and in vivo drug screening in a relatively higher-throughput and more objective manner. 2, The individual platform consists of an infrared array, a vibration floor sensor and a variety of modular devices. One computer can simultaneously operate up to 16 platforms via USB cables. 3. The SmartCage(™) detects drug-induced increases and decreases in activity levels, as well as changes in movement patterns. Wake and sleep states of mice can be detected using the vibration floor sensor. The arousal state classification achieved up to 98% accuracy compared with results obtained by electroencephalography and electromyography. More complex behaviours, including motor coordination, anxiety-related behaviours and social approach behaviour, can be assessed using appropriate modular devices and the results obtained are comparable with results obtained using conventional methods. 4. In conclusion, the SmartCage(™) system provides an automated and accurate tool to quantify various rodent behaviours in a 'stress-free' environment. This system, combined with the validated testing protocols, offers powerful a tool kit for transgenic phenotyping and in vivo drug screening.
authors.affiliation: SRI International, Menlo Park, CA, USA.
authors.forename: Taline V
authors.initials: TV
authors.lastname: Khroyan
authors.forename: Jingxi
authors.initials: J
authors.lastname: Zhang
authors.forename: Liya
authors.initials: L
authors.lastname: Yang
authors.forename: Bende
authors.initials: B
authors.lastname: Zou
authors.forename: James
authors.initials: J
authors.lastname: Xie
authors.forename: Conrado
authors.initials: C
authors.lastname: Pascual
authors.forename: Adam
authors.initials: A
authors.lastname: Malik
authors.forename: Julian
authors.initials: J
authors.lastname: Xie
authors.forename: Nurulain T
authors.initials: NT
authors.lastname: Zaveri
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Vazquez
authors.forename: Willma
authors.initials: W
authors.lastname: Polgar
authors.forename: Lawrence
authors.initials: L
authors.lastname: Toll
authors.forename: Jidong
authors.initials: J
authors.lastname: Fang
authors.forename: Xinmin
authors.initials: X
authors.lastname: Xie
mesh_terms: D000818:Animals; D057205:Automation, Laboratory / Q000295:instrumentation* / Q000379:methods; D001522:Behavior, Animal; D005260:Female; D006799:Housing, Animal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D009048:Motor Skills; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep; D014851:Wakefulness