title: Effect of annexin-A1 peptide treatment during lung inflammation induced by lipopolysaccharide.
abstract: Lung endotoxemia is characterized by neutrophil accumulation, increased vascular permeability and parenchymal injury. This can also affect the endogenous pathways that operate in the host to keep inflammation under control. Here, we demonstrate differential expression of annexin-A1 (AnxA1) protein in mice after the local or intraperitoneal administration of lipopolysaccharide (LPS; 1 mg/kg) in mice and the regulation of the endotoxemic inflammation after the pre-treatment with the AnxA1 peptidomimetic Ac2-26. The intranasal administration of LPS induced the leukocyte migration and cytokine release to the alveolar space, whereas the peritoneal administration of LPS generated a deregulated cellular and cytokine response, with a marked degree of leukocyte adhesion in the microcirculation. The peptide Ac2-26 pre-treatment inhibited the leukocyte migration and the pro-inflammatory cytokine release. Also, it induced the expression of endogenous AnxA1 and the anti-inflammatory cytokine IL-10. In conclusion, our data obtained from endotoxemia induced by local or intraperitoneal LPS administration suggested that the molecular mechanisms induced by AnxA1 peptidomimetic Ac2-26 lead to the regulation of leukocyte activation/migration and cytokine production induced by LPS.
authors.affiliation: Department of Biology, Instituto de Biociências, Letras e Ciências Exatas, São Paulo State University, 15054-000 São José do Rio Preto, SP, Brazil.
authors.forename: Esther Emanuella
authors.initials: EE
authors.lastname: da Cunha
authors.forename: Sonia Maria
authors.initials: SM
authors.lastname: Oliani
authors.forename: Amílcar Sabino
authors.initials: AS
authors.lastname: Damazo
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000378:metabolism / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002448:Cell Adhesion; D002465:Cell Movement; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D008168:Lung / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000494:pharmacology; D011014:Pneumonia / Q000139:chemically induced / Q000276:immunology / Q000503:physiopathology*
chemical_list: D017305:Annexin A1; D016207:Cytokines; D008070:Lipopolysaccharides; D010455:Peptides; C096153:annexin A1 peptide (2-26)