title: Characterization of the antibiotic compound no. 70 produced by Streptomyces sp. IMV-70.
abstract: We describe the actinomycete strain IMV-70 isolated from the soils of Kazakhstan, which produces potent antibiotics with high levels of antibacterial activity. After the research of its morphological, chemotaxonomic, and cultural characteristics, the strain with potential to be developed further as a novel class of antibiotics with chemotherapeutics potential was identified as Streptomyces sp. IMV-70. In the process of fermentation, the strain Streptomyces spp. IMV-70 produces the antibiotic no. 70, which was isolated from the culture broth by extraction with organic solvents. Antibiotic compound no. 70 was purified and separated into individual components by HPLC, TLC, and column chromatography methods. The main component of the compound is the antibiotic 70-A, which was found to be identical to the peptolide etamycin A. Two other antibiotics 70-B and 70-C have never been described and therefore are new antibiotics. The physical-chemical and biological characteristics of these preparations were described and further researched. Determination of the optimal growth conditions to cultivate actinomycete-producer strain IMV-70 and development of methods to isolate, purify, and accumulate preparations of the new antibiotic no. 70 enable us to research further the potential of this new class of antibiotics.
authors.affiliation: Institute of Microbiology and Virology, Ministry of Education and Science Committee, 103, Bogenbay batyr Street, Almaty, Kazakhstan.
authors.forename: Lyudmila P
authors.initials: LP
authors.lastname: Trenozhnikova
authors.forename: Almagul K
authors.initials: AK
authors.lastname: Khasenova
authors.forename: Assya S
authors.initials: AS
authors.lastname: Balgimbaeva
authors.forename: Galina B
authors.initials: GB
authors.lastname: Fedorova
authors.forename: Genrikh S
authors.initials: GS
authors.lastname: Katrukha
authors.forename: Nina L
authors.initials: NL
authors.lastname: Tokareva
authors.forename: Boo H
authors.initials: BH
authors.lastname: Kwa
authors.forename: Azliyati
authors.initials: A
authors.lastname: Azizan
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D012988:Soil Microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000378:metabolism*; D047630:Depsipeptides
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; C004910:etamycin; D047630:Depsipeptides