title: Phylogenetic analysis of partial RNA-polymerase blocks II and III of Rabies                      virus isolated from the main rabies reservoirs in Brazil.
abstract: This study describes the results of the sequencing and analysis of segments of Blocks II and III of the RNA polymerase L gene of Rabies virus isolates from different reservoir species of Brazil. The phylogenetic relations of the virus were determined and a variety of species-specific nucleotides were found in the analyzed areas, but the majority of these mutations were found to be synonymous. However, an analysis of the putative amino acid sequences were shown to have some characteristic mutations between some reservoir species of Brazil, indicating that there was positive selection in the RNA polymerase L gene of Rabies virus. On comparing the putative viral sequences obtained from the Brazilian isolates and other Lyssavirus, it was determined that amino acid mutations occurred in low-restriction areas. This study of the L gene of Rabies virus is the first to be conducted with samples of virus isolates from Brazil, and the results obtained will help in the determination of the phylogenetic relations of the virus.
authors.affiliation: Pasteur Institute, Av. Paulista 393, São Paulo, Brazil. pedrocarnielijunio@ymail.com
authors.forename: Pedro
authors.initials: P
authors.lastname: Carnieli
authors.forename: Rafael
authors.initials: R
authors.lastname: de Novaes Oliveira
authors.forename: Willian
authors.initials: W
authors.lastname: de Oliveira Fahl
authors.forename: Helena Beatriz
authors.initials: HB
authors.lastname: de Carvalho Ruthner Batista
authors.forename: Karin Corrêa
authors.initials: KC
authors.lastname: Scheffer
authors.forename: Keila
authors.initials: K
authors.lastname: Iamamoto
authors.forename: Juliana Galera
authors.initials: JG
authors.lastname: Castilho
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D046028:Canidae / Q000821:virology; D002685:Chiroptera / Q000821:virology; D004197:Disease Reservoirs / Q000821:virology*; D004285:Dogs; D018114:Lyssavirus / Q000145:classification / Q000235:genetics; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D011818:Rabies / Q000453:epidemiology* / Q000821:virology; D011820:Rabies virus / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D012324:RNA-Dependent RNA Polymerase