title: Kinetic analysis of biodesulfurization of model oil containing multiple alkyl dibenzothiophenes.
abstract: Biodesulfurization is regarded as a promising alternative technology for desulfurization from diesel oil due to its mild operating conditions and its ability to remove sulfur from alky dibenzothiophenes (C(x)-DBTs). The diesel oil contains complex mixtures of C(x)-DBTs in which individual microbial biodesulfurization may be altered. In this work, interactions among three typical C(x)-DBTs such as dibenzothiophenes (DBT), 4-methyldibenzothiophene (4-MDBT), and 4,6-dimethyldibenzothiophene (4,6-DMDBT) were investigated using Mycobacterium sp. ZD-19 in an airlift reactor. The experimental results indicated that the desulfurization rates would decrease in the multiple C(x)-DBTs system compared to the single C(x)-DBT system. The extent of inhibition depended upon the substrate numbers, concentrations, and affinities of the co-existing substrates. For example, compared to individual desulfurization rate (100 %), DBT desulfurization rate decreased to 75.2 % (DBT + 4,6-DMDBT), 64.8 % (DBT + 4-MDBT), and 54.7 % (DBT + 4,6-DMDBT + 4-MDBT), respectively. This phenomenon was caused by an apparent competitive inhibition of substrates, which was well predicted by a Michaelis-Menten competitive inhibition model.
authors.affiliation: Institute of Environmental Engineering, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
authors.forename: Shi-Han
authors.initials: SH
authors.lastname: Zhang
authors.forename: Han
authors.initials: H
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D001711:Biotransformation; D005742:Gasoline; D007700:Kinetics; D009161:Mycobacterium / Q000254:growth & development / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*
chemical_list: D005742:Gasoline; D013876:Thiophenes; D013455:Sulfur; C016366:dibenzothiophene