title: [Microbiological diagnosis of mycoplasma infections].
abstract: The microbiological diagnosis of mycoplasma and ureaplasma infections has always been limited due to the fastidious growth of these microorganisms, as well as the lack of commercially prepared growth media, absence of rapid diagnostic procedures, and the clinical perception that these organisms are less significant in the infectious diseases setting. During the last few years, this situation has substantially improved due to the commercial availability of culture media, the development of rapid serological techniques, and, in particular, to the introduction of nucleic acid amplification assays, commercially available or "in-house" preparations. Despite the lack of proper standardisation and validation of the molecular and serological techniques, methodological advances have led to an increased detection of these microorganisms and, consequently, a greater appreciation of their clinical relevance.
authors.affiliation: Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Madrid, España. mmeseguer.hrc@salud.madrid.org
authors.forename: María Antonia
authors.initials: MA
authors.lastname: Meseguer-Peinado
authors.forename: Beatriz
authors.initials: B
authors.lastname: Acosta-Boga
authors.forename: Lurdes
authors.initials: L
authors.lastname: Matas-Andreu
authors.forename: Gemma
authors.initials: G
authors.lastname: Codina-Grau
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D001431:Bacteriological Techniques; D003470:Culture Media; D004269:DNA, Bacterial / Q000097:blood; D005260:Female; D052776:Female Urogenital Diseases / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases / Q000175:diagnosis / Q000382:microbiology; D013058:Mass Spectrometry; D009174:Mycoplasma / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis / Q000382:microbiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction; D012698:Serologic Tests / Q000379:methods; D013045:Species Specificity; D013048:Specimen Handling; D013059:Spectrum Analysis, Raman; D014509:Ureaplasma / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity; D016869:Ureaplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D014774:Virulence
chemical_list: D000907:Antibodies, Bacterial; D003470:Culture Media; D004269:DNA, Bacterial