title: Seroprevalence and isolation of Toxoplasma gondii from free-range chickens from Espírito Santo state, southeastern Brazil.
abstract: Prevalence of Toxoplasma gondii infection in 510 free-range (FR) chickens (380 from 33 small farms, and 130 from a slaughter house for FR chickens) from Espírito Santo state, southeastern Brazil, was investigated. Antibodies to T. gondii were sought using commercial indirect haemagglutination (IHAT, Imuno-HAI Toxo(®), Wama Diagnóstica, São Paulo, Brazil, cut-off 1:16) and the modified agglutination test (MAT, cut-off 1:25) tests. Attempts were made to isolate viable T. gondii from seropositive chickens by bioassay in mice. Pooled samples of brain, heart and quadriceps muscle of one thigh (total 40 g) from 64 chickens with IHAT titers of ≥ 1:16 were minced, digested in pepsin and bioassayed in mice. Antibodies to T. gondii were found in 40.4% (206/510) FR chickens by IHAT (titer ≥ 1:16) and 38.8% (198/510) by MAT (titer ≥ 1:25); concordance between IHAT and MAT was 81.6% (kappa index=0.614). Viable T. gondii was isolated (designated TgCkBr234-281) from 48 of 64 (75%) seropositive (IHAT titers ≥ 1:32) FR chickens. Most isolates of T. gondii were virulent for mice; 100% of mice inoculated with 44 of 48 isolates died of toxoplasmosis within 30 days post inoculation (p.i). An epidemiological investigation revealed that people living in rural areas have little knowledge about the parasite and about the risk of acquiring it from raw meat. Results indicated that the locally available IHAT was useful for screening of chicken sera for T. gondii antibodies.
authors.affiliation: Curso de Medicina Veterinária, Universidade de Vila Velha, Rua Viana, s/n, Bairro Boa Vista, Vila Velha, CEP 29102-690, ES, Brazil.
authors.forename: M A V
authors.initials: MA
authors.lastname: Beltrame
authors.forename: H F J
authors.initials: HF
authors.lastname: Pena
authors.forename: N C
authors.initials: NC
authors.lastname: Ton
authors.forename: A J B
authors.initials: AJ
authors.lastname: Lino
authors.forename: S M
authors.initials: SM
authors.lastname: Gennari
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: F E L
authors.initials: FE
authors.lastname: Pereira
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001681:Biological Assay / Q000662:veterinary; D001921:Brain / Q000469:parasitology; D001938:Brazil / Q000453:epidemiology; D002645:Chickens / Q000469:parasitology*; D005260:Female; D006321:Heart / Q000469:parasitology; D006386:Hemagglutination Tests / Q000662:veterinary; D051379:Mice; D009132:Muscles / Q000469:parasitology; D011201:Poultry Diseases / Q000453:epidemiology / Q000469:parasitology*; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000453:epidemiology / Q000469:parasitology*
chemical_list: D000913:Antibodies, Protozoan