title: Immunogenicity of Japanese encephalitis virus envelope protein by Hyphantria cunea nuclear polyhedrosis virus vector in guinea pig.
abstract: Japanese encephalitis virus (JEV) is an important pathogen causing febrile syndrome, encephalitis, and death. Envelop (E) glycoprotein is the major target of inducing neutralizing antibodies and protective immunity in host. In this study, E glycoprotein of JEV was expressed in Spodoptera frugiperd 9 cells as a fusion protein containing a gX signal sequence of pseudorabies virus. This purified HcE recombinant protein was evaluated for their immunogenicity and protective efficacy in guinea pig. The survival rates of guinea pig immunized with HcE protein was significantly increased over that of JE vaccine. This result indicates helpful information for developing a subunit vaccine against JEV.
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju 380-701, Korea.
authors.forename: Hyung-Hoan
authors.initials: HH
authors.lastname: Lee
authors.forename: Seung-Kuk
authors.initials: SK
authors.lastname: Hong
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Yoon
authors.forename: Sung-Jae
authors.initials: SJ
authors.lastname: Jang
authors.forename: Young-Yil
authors.initials: YY
authors.lastname: Bahk
authors.forename: Min-Dong
authors.initials: MD
authors.lastname: Song
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Chan-Gil
authors.initials: CG
authors.lastname: Kim
authors.forename: Bokyung
authors.initials: B
authors.lastname: Kim
authors.forename: Tae-Kyu
authors.initials: TK
authors.lastname: Park
authors.forename: Hyun
authors.initials: H
authors.lastname: Kang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology / Q000821:virology*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; C080628:glycoprotein E, Japanese encephalitis virus