title: High HSP27 and HSP70 expression levels are independent adverse prognostic factors in primary resected colon cancer.
abstract: BACKGROUND
  The expression of Heat Shock Proteins (HSPs) is increased in various cancers and has been shown to correlate with biological tumor behaviour. This study aimed to investigate the impact of HSP70, HSP60 and HSP27 expression in colon cancer.
  
  
  METHODS
  HSP expression was determined by immunohistochemistry on a tissue microarray with 355 primary resected colon carcinomas of all stages. Expression patterns were correlated with pathologic features (UICC pTNM category, tumor grading) and survival.
  
  
  RESULTS
  Expression of HSP27, HSP60 and HSP70 ranged from negative to high. There was no correlation between HSP27, HSP60 and HSP70 expression among each other and with UICC pT category, presence of lymph node or distant metastases or tumor grading. High HSP70 expression was associated with worse overall survival (p < 0.001) and was an independent prognostic factor (p = 0.004) in multivariate analysis including the pathological parameters mentioned above. For patients without lymph node or distant metastases (UICC stages I/II) and with complete tumor excision, HSP70 expression was the only independent prognostic factor for survival (p = 0.001) and superior to UICC pT category. In left sided UICC stage I/II carcinomas, high HSP27 expression also had adverse prognostic impact and was an independent prognostic factor (p = 0.016) besides HSP70 (p = 0.002).
  
  
  CONCLUSIONS
  High HSP70 and HSP27 expression is associated with worse clinical outcome in colon cancer. Determination of tumoral HSP70 and HSP27 may be used as additional biomarker for risk stratification especially for UICC stage I/II patients.
authors.affiliation: Institute of Pathology, Technische Universität München, Trogerstr. 18, 81675 Munich, Germany.
authors.forename: Karina
authors.initials: K
authors.lastname: Bauer
authors.forename: Ulrich
authors.initials: U
authors.lastname: Nitsche
authors.forename: Julia
authors.initials: J
authors.lastname: Slotta-Huspenina
authors.forename: Enken
authors.initials: E
authors.lastname: Drecoll
authors.forename: Claus Hann
authors.initials: CH
authors.lastname: von Weyhern
authors.forename: Robert
authors.initials: R
authors.lastname: Rosenberg
authors.forename: Heinz
authors.initials: H
authors.lastname: Höfler
authors.forename: Rupert
authors.initials: R
authors.lastname: Langer
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018834:Chaperonin 60 / Q000378:metabolism; D003110:Colonic Neoplasms / Q000473:pathology* / Q000601:surgery*; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D015999:Multivariate Analysis; D011379:Prognosis; D016019:Survival Analysis
chemical_list: D018834:Chaperonin 60; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones