title: Intra-specific genetic diversity in wild olives (Olea  europaea ssp cuspidata) in Hormozgan Province, Iran.
abstract: Wild olive (O. europaea ssp cuspidata)  plants grow in various regions of Iran and are expected to have considerable  genetic diversity due to adaptation to the various environmental conditions. We  examined the genetic diversity of four populations of wild olive growing in  Hormozgan Province located in southern Iran by using 30 RAPDs and 10 ISSR  markers. The mean value of polymorphism for RAPD loci was 73.71%, while the  value for ISSR loci was 81.74%. The Keshar population had the highest value of  intra-population polymorphism for both RAPD and ISSR loci (66.86 and 62.71%,  respectively), while the Tudar population had the lowest values (20.35 and  28.81%, respectively). Similarly, the highest and lowest number of effective  alleles, Shannon index and Nei's genetic diversity were also found for these  two populations. The highest value of H(pop)/H(sp) within  population genetic diversity for RAPD and ISSR loci was found for the Keshar  population (H(pop) = 0.85 and H(sp) = 0.90). OPA04-750, OPA13-650 and OPA02-350 RAPD bands  were specific for Tudar, Bondon and Keshar populations, respectively, while no  specific ISSR bands were observed. Analysis of molecular variance as well as  the pairwise F(ST) test showed significant differences for  RAPD and ISSR markers among the populations. The NJ and UPGMA trees also  separated the wild olive populations from each other, indicating their genetic  distinctness. UPGMA clustering of the four wild olive populations placed the  Tudar population far from the other populations; Keshar and Bokhoon population samples  revealed more similarity and were grouped together. We conclude that there is  high genetic diversity among O. europaea ssp cuspidata populations  located in southern Iran. We also found RAPD and ISSR markers to be useful  molecular tools to discriminate and evaluate genetic variations in wild olive  trees.
authors.affiliation: Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. z-nouri@srbiau.ac.ir
authors.forename: Z
authors.initials: Z
authors.lastname: Noormohammadi
authors.forename: H
authors.initials: H
authors.lastname: Samadi-Molayousefi
authors.forename: M
authors.initials: M
authors.lastname: Sheidai
mesh_terms: D014644:Genetic Variation; D007492:Iran; D018895:Microsatellite Repeats; D031658:Olea / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D058974:Phylogeography; D019105:Random Amplified Polymorphic DNA Technique