title: Early stage foreign body reaction against biodegradable polymer scaffolds affects tissue regeneration during the autologous transplantation of tissue-engineered cartilage in the canine model.
abstract: To overcome the weak points of the present cartilage regenerative medicine, we applied a porous scaffold for the production of tissue-engineered cartilage with a greater firmness and a 3D structure. We combined the porous scaffolds with atelocollagen to retain the cells within the porous body. We conducted canine autologous chondrocyte transplants using biodegradable poly-L-lactic acid (PLLA) or poly-DL-lactic-co-glycolic acid (PLGA) polymer scaffolds, and morphologically and biochemically evaluated the time course changes of the transplants. The histological findings showed that the tissue-engineered constructs using PLLA contained abundant cartilage 1, 2, and 6 months after transplantation. However, the PLGA constructs did not possess cartilage and could not maintain their shapes. Biochemical measurement of the proteoglycan and type II collagen also supported the superiority of PLLA. The biodegradation of PLGA progressed much faster than that of PLLA, and the PLGA had almost disappeared by 2 months. The degraded products of PLGA may evoke a more severe tissue reaction at this early stage of transplantation than PLLA. The PLLA scaffolds were suitable for cartilage tissue engineering under immunocompetent conditions, because of the retarded degradation properties and the decrease in the severe tissue reactions during the early stage of transplantation.
authors.affiliation: Department of Cartilage & Bone Regeneration (Fujisoft), Tokyo University Graduate School of Medicine, Tokyo, Japan.
authors.forename: Yukiyo
authors.initials: Y
authors.lastname: Asawa
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Komura
authors.forename: Makoto
authors.initials: M
authors.lastname: Watanabe
authors.forename: Satoru
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takazawa
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takato
authors.forename: Kazuto
authors.initials: K
authors.lastname: Hoshi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*; D002356:Cartilage / Q000166:cytology / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology / Q000637:transplantation; D024043:Collagen Type II / Q000378:metabolism; D004285:Dogs; D005549:Foreign-Body Reaction / Q000473:pathology*; D006025:Glycosaminoglycans / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism; D008297:Male; D011091:Polyesters; D011100:Polyglycolic Acid / Q000737:chemistry / Q000378:metabolism; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D012038:Regeneration; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014182:Transplantation, Autologous
chemical_list: D001672:Biocompatible Materials; D024043:Collagen Type II; D006025:Glycosaminoglycans; D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide)