title: Immunophenotyping of posttraumatic neutrophils on a routine haematology analyser.
abstract: BACKGROUND
  Flow cytometry markers have been proposed as useful predictors for the occurrence of posttraumatic inflammatory complications. However, currently the need for a dedicated laboratory and the labour-intensive analytical procedures make these markers less suitable for clinical practice. We tested an approach to overcome these limitations.
  
  
  METHODS
  Neutrophils of healthy donors were incubated with antibodies commonly used in trauma research: CD11b (MAC-1), L-selectin (CD62L), FcγRIII (CD16), and FcγRII (CD32) in active form (MoPhab A27). Flow cytometric analysis was performed both on a FACSCalibur, a standard flow cytometer, and on a Cell-Dyn Sapphire, a routine haematology analyser.
  
  
  RESULTS
  There was a high level of agreement between the two types of analysers, with 41% for FcγRIII, 80% for L-selectin, 98% for CD11b, and even a 100% agreement for active FcγRII. Moreover, analysis on the routine haematology analyser was possible in less than a quarter of the time in comparison to the flow cytometer.
  
  
  CONCLUSIONS
  Analysis of neutrophil phenotype on the Cell-Dyn Sapphire leads to the same conclusion compared to a standard flow cytometer. The markedly reduced time necessary for analysis and reduced labour intensity constitutes a step forward in implementation of this type of analysis in clinical diagnostics in trauma research.
authors.affiliation: Department of Trauma Surgery, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands. k.m.groeneveld@umcutrecht.nl
authors.forename: Kathelijne Maaike
authors.initials: KM
authors.lastname: Groeneveld
authors.forename: Marjolein
authors.initials: M
authors.lastname: Heeres
authors.forename: Loek Petrus Hendrikus
authors.initials: LP
authors.lastname: Leenen
authors.forename: Albert
authors.initials: A
authors.lastname: Huisman
authors.forename: Leo
authors.initials: L
authors.lastname: Koenderman
mesh_terms: D039481:CD11b Antigen / Q000378:metabolism; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000378:metabolism; D006405:Hematology / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D019041:L-Selectin / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology* / Q000276:immunology*; D017452:Receptors, IgG / Q000378:metabolism
chemical_list: D039481:CD11b Antigen; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D017452:Receptors, IgG; D019041:L-Selectin