title: Molecular cloning and characterization of a nonsymbiotic hemoglobin gene (GLB1) from Malus hupehensis Rehd. with heterologous expression in tomato.
abstract: Nonsymbiotic hemoglobins (nsHbs) are involved in a variety of cellular processes in plants. Previous studies indicate that nsHb expression improves plant tolerance during waterlogging and hypoxia. In the present work, the nsHb class-1 coding sequence was cloned from Malus hupehensis Rehd. var. pinyiensis Jiang and subsequently named MhGLB1. The results elucidated the expressed characteristics and physiological effects of MhGLB1. The full-length cDNA contained a 477 bp open reading frame encoding a protein with a molecular mass of 17.8 KDa with 158 amino acids. Quantitative real-time PCR analysis showed that MhGLB1 expresses in roots, stems and leaves growing under normal and nitrate-induced conditions. Hypoxic stress induced accumulation of MhGLB1 within 12 h, and abscisic acid significantly induced expression of MhGLB1 in roots. The photosynthetic, transpiration and stomatal conductance rates of transgenic MhGLB1 tomato plants decreased more slowly than that of wild-type plants under waterlogging treatment. These results indicated that the MhGLB1 gene has an important role in hypoxia.
authors.affiliation: State Key Laboratory of Corp Biology/College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China.
authors.forename: Xingzheng
authors.initials: X
authors.lastname: Shi
authors.forename: Xinliang
authors.initials: X
authors.lastname: Wang
authors.forename: Futian
authors.initials: F
authors.lastname: Peng
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D003001:Cloning, Molecular; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006454:Hemoglobins / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D027845:Malus / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D030821:Plants, Genetically Modified; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological
chemical_list: D006454:Hemoglobins; D000040:Abscisic Acid