title: Engraftment of human adipose derived stem cells delivered in a hyaluronic acid preparation in mice.
abstract: OBJECTIVE
  To evaluate the implant of human adipose derived stem cells (ADSC) delivered in hyaluronic acid gel (HA), injected in the subcutaneous of athymic mice.
  
  
  METHODS
  Control implants -HA plus culture media was injected in the subcutaneous of the left sub scapular area of 12 athymic mice. ADSC implants: HA plus ADSC suspended in culture media was injected in the subcutaneous, at the contra lateral area, of the same animals. With eight weeks, animals were sacrificed and the recovered implants were processed for extraction of genomic DNA, and histological study by hematoxilin-eosin staining and immunufluorescence using anti human vimentin and anti von Willebrand factor antibodies.
  
  
  RESULTS
  
  
  
  METHODS
  Not visualized at the injection site. An amorphous substance was observed in hematoxilin-eosin stained sections. Human vimentin and anti von Willebrand factor were not detected. No human DNA was detected. ADSC implants - A plug was visible at the site of injection. Fusiform cells were observed in sections stained by hematoxilin- eosin and both human vimentin and anti von Willebrand factor were detected by immunofluorescence. The presence of human DNA was confirmed.
  
  
  CONCLUSIONS
  The delivery of human adipose derived stem cells in preparations of hyaluronic acid assured cells engraftment at the site of injection.
authors.affiliation: Department of Surgery, Laboratory of Surgical Anatomy of Human Structural Topography, School of Medicine, Sao Paulo University, Brazil. isa.dietrich@isa.med.br
authors.forename: Isa
authors.initials: I
authors.lastname: Dietrich
authors.forename: Olivia
authors.initials: O
authors.lastname: Cochet
authors.forename: Phi
authors.initials: P
authors.lastname: Villageois
authors.forename: Consuelo Junqueira
authors.initials: CJ
authors.lastname: Rodrigues
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000637:transplantation*; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D020007:Implants, Experimental; D007279:Injections, Subcutaneous / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D023421:Models, Animal; D033581:Stem Cell Transplantation / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods; D014746:Vimentin / Q000032:analysis; D014841:von Willebrand Factor / Q000032:analysis / Q000037:antagonists & inhibitors
chemical_list: D014746:Vimentin; D014841:von Willebrand Factor; D006820:Hyaluronic Acid