title: Human erythrocytes and neuroblastoma cells are in vitro affected by sodium orthovanadate.
abstract: Research on biological influence of vanadium has gained major importance because it exerts potent toxic, mutagenic, and genotoxic effects on a wide variety of biological systems. However, hematological toxicity is one of the less studied effects. The lack of information on this issue prompted us to study the structural effects induced on the human erythrocyte membrane by vanadium (V). Sodium orthovanadate was incubated with intact erythrocytes, isolated unsealed human erythrocyte membranes (IUM) and molecular models of the erythrocyte membrane. The latter consisted of bilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. This report presents evidence in order that orthovanadate interacted with red cell membranes as follows: a) in scanning electron microscopy (SEM) studies it was observed that morphological changes on human erythrocytes were induced; b) fluorescence spectroscopy experiments in isolated unsealed human erythrocyte membranes (IUM) showed that an increase in the molecular dynamics and/or water content at the shallow depth of the lipids glycerol backbone at concentrations as low as 50μM was produced; c) X-ray diffraction studies showed that orthovanadate 0.25-1mM range induced increasing structural perturbation to DMPE; d) somewhat similar effects were observed by differential scanning calorimetry (DSC) with the exception of the fact that DMPC pretransition was shown to be affected; and e) fluorescence spectroscopy experiments performed in DMPC large unilamellar vesicles (LUV) showed that at very low concentrations induced changes in DPH fluorescence anisotropy at 18°C. Additional experiments were performed in mice cholinergic neuroblastoma SN56 cells; a statistically significant decrease of cell viability was observed on orthovanadate in low or moderate concentrations.
authors.affiliation: Faculty of Chemical Sciences, University of Concepción, Concepción, Chile. msuwalsk@udec.cl
authors.forename: M
authors.initials: M
authors.lastname: Suwalsky
authors.forename: P
authors.initials: P
authors.lastname: Fierro
authors.forename: F
authors.initials: F
authors.lastname: Villena
authors.forename: L F
authors.initials: LF
authors.lastname: Aguilar
authors.forename: C P
authors.initials: CP
authors.lastname: Sotomayor
authors.forename: M
authors.initials: M
authors.lastname: Jemiola-Rzeminska
authors.forename: K
authors.initials: K
authors.lastname: Strzalka
authors.forename: S
authors.initials: S
authors.lastname: Gul-Hinc
authors.forename: A
authors.initials: A
authors.lastname: Ronowska
authors.forename: A
authors.initials: A
authors.lastname: Szutowicz
mesh_terms: D000105:Acetyl Coenzyme A / Q000737:chemistry; D000818:Animals; D016880:Anisotropy; D002152:Calorimetry, Differential Scanning / Q000379:methods; D045744:Cell Line, Tumor; D002470:Cell Survival; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009447:Neuroblastoma / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D012964:Sodium / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013696:Temperature; D053835:Unilamellar Liposomes / Q000737:chemistry; D014638:Vanadates / Q000494:pharmacology*; D014639:Vanadium / Q000494:pharmacology
chemical_list: D008055:Lipids; D010714:Phosphatidylethanolamines; D053835:Unilamellar Liposomes; D014639:Vanadium; D014638:Vanadates; D000105:Acetyl Coenzyme A; D012964:Sodium; D004134:Dimyristoylphosphatidylcholine; C018525:1,2-dimyristoylphosphatidylethanolamine