title: Single-injection HPLC method for rapid analysis of a combination drug delivery system.
abstract: Developing combination drug delivery systems (CDDS) is a challenging but necessary task to meet the needs of complex therapy regimes for patients. As the number of multi-drug regimens being administered increases, so does the difficulty of characterizing the CDDS as a whole. We present a single-step method for quantifying three model therapeutics released from a model hydrogel scaffold using high-performance liquid chromatography (HPLC). Poly(ethylene glycol) dimethacrylate (PEGDMA) hydrogel tablets were fabricated via photoinitiated crosslinking and subsequently loaded with model active pharmaceutical ingredients (APIs), namely, porcine insulin (PI), fluorescein isothiocyanate-labeled bovine serum albumin (FBSA), prednisone (PSE), or a combination of all three. The hydrogel tablets were placed into release chambers and sampled over 21 days, and APIs were quantified using the method described herein. Six compounds were isolated and quantified in total. Release kinetics based on chemical properties of the APIs did not give systematic relationships; however, PSE was found to have improved device loading versus PI and FBSA. Rapid analysis of three model APIs released from a PEGDMA CDDS was achieved with a direct, single-injection HPLC method. Development of CDDS platforms is posited to benefit from such analytical approaches, potentially affording innovative solutions to complex disease states.
authors.affiliation: Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California 94158, USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Tucker
authors.forename: Benjamin W
authors.initials: BW
authors.lastname: Parcher
authors.forename: Ella F
authors.initials: EF
authors.lastname: Jones
authors.forename: Tejal A
authors.initials: TA
authors.lastname: Desai
mesh_terms: D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004338:Drug Combinations; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D020100:Hydrogels; D057927:Hydrophobic and Hydrophilic Interactions; D061389:Insulin, Regular, Pork / Q000008:administration & dosage / Q000737:chemistry*; D007700:Kinetics; D008689:Methacrylates / Q000737:chemistry* / Q000528:radiation effects; D008970:Molecular Weight; D055668:Photochemical Processes; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000528:radiation effects; D011241:Prednisone / Q000008:administration & dosage / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry*; D012995:Solubility; D013607:Tablets; D013678:Technology, Pharmaceutical / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004338:Drug Combinations; D020100:Hydrogels; D061389:Insulin, Regular, Pork; D008689:Methacrylates; D013607:Tablets; C039217:fluorescein isothiocyanate bovine serum albumin; C421283:poly(ethylene glycol)-dimethacrylate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate; D011241:Prednisone