title: Centrally administered bombesin activates COX-containing spinally projecting neurons of the PVN in anesthetized rats.
abstract: The paraventricular nucleus (PVN) of the hypothalamus has a heterogenous structure containing different types of output neurons that project to the median eminence, posterior pituitary, brain stem autonomic centers and sympathetic preganglionic neurons in the spinal cord. Presympathetic neurons in the PVN send mono- and poly-synaptic projections to the spinal cord. In the present study using urethane-anesthetized rats, we examined the effects of centrally administered bombesin (a homologue of the mammalian gastrin-releasing peptide) on the mono-synaptic spinally projecting PVN neurons pre-labeled with a retrograde tracer Fluoro-Gold (FG) injected into T8 level of the spinal cord, with regard to the immunoreactivity for cyclooxygenase (COX) isozymes (COX-1/COX-2) and Fos (a marker of neuronal activation). FG-labeled spinally projecting neurons were abundantly observed in the dorsal cap, ventral part and posterior part of the PVN. The immunoreactivity of each COX-1 and COX-2 was detected in FG-labeled spinally projecting PVN neurons in the vehicle (10 μl of saline/animal, i.c.v.)-treated group, while bombesin (1 nmol/animal, i.c.v.) had no effect on the number of these immunoreactive neurons for each COX isozyme with labeling of FG. On the other hand, the peptide significantly increased the number of double-immunoreactive neurons for Fos and COX-1/COX-2 with FG-labeling in the PVN (except triple-labeled neurons for FG, COX-2 and Fos in the dorsal cap of the PVN), as compared to those of vehicle-treated group. These results suggest that centrally administered bombesin activates spinally projecting PVN neurons containing COX-1 and COX-2 in rats.
authors.affiliation: Department of Pharmacology, School of Medicine, Kochi University, Nankoku, Kochi 783-8505, Japan. tanakak@kochi-u.ac.jp
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Tanaka
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shimizu
authors.forename: Lianyi
authors.initials: L
authors.lastname: Lu
authors.forename: Kumiko
authors.initials: K
authors.lastname: Nakamura
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Yokotani
mesh_terms: D000818:Animals; D017776:Autonomic Pathways / Q000201:enzymology; D001839:Bombesin / Q000008:administration & dosage / Q000494:pharmacology*; D051545:Cyclooxygenase 1 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D007276:Injections, Intraventricular; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000201:enzymology; D010286:Paraventricular Hypothalamic Nucleus / Q000033:anatomy & histology / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology
chemical_list: D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D001839:Bombesin