title: Bovine viral diarrhea virus: correlation between herd seroprevalence and bulk tank milk antibody levels using 4 commercial immunoassays.
abstract: The aim of the present study was to establish a relationship between the results obtained using the enzyme-linked immunosorbent assay (ELISA) technique for antibodies in blood serum and milk at herd level. For this purpose, 325 samples of bulk tank milk were analyzed with 4 antibody ELISAs from dairy herds with a prevalence of seropositive animals; seroprevalence was also evaluated. Data were arranged to analyze the sensitivity of the bulk tank milk test to detect herds with high risk of active infection (>65% seroprevalence) and the specificity to detect those with very few (<5%) or no (0%) seropositive animals, respectively. The sensitivity values ranged from 0.92 to 0.70 and the specificity from 0.83 to 0.54 to detect free herds (0% seroprevalence) and from 0.88 to 0.77 to detect herds with <5% of seropositive animals. In a quantitative approach, Pearson correlation coefficients, reported as a measure of linear association between herd seroprevalence and transformed optical density values recorded in bulk tank milk, ranged from 0.71 to 0.86. According to these results, the 4 antibody ELISAs would be valid tests for carrying out a herd classification program using milk samples.
authors.affiliation: Laboratorio de Sanidade e Produción Animal de Galicia, Avda de Madrid 77, 27002 Lugo, Spain. carmen.eiras.ferreiro@xunta.es
authors.forename: Carmen
authors.initials: C
authors.lastname: Eiras
authors.forename: Ignacio
authors.initials: I
authors.lastname: Arnaiz
authors.forename: María L
authors.initials: ML
authors.lastname: Sanjuán
authors.forename: Eduardo
authors.initials: E
authors.lastname: Yus
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Diéguez
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008892:Milk / Q000821:virology*; D015995:Prevalence; D012680:Sensitivity and Specificity; D013030:Spain / Q000453:epidemiology; D018709:Statistics, Nonparametric
chemical_list: D000914:Antibodies, Viral