title: Purification of human and avian influenza viruses using cellulose sulfate ester (Cellufine Sulfate) in the process of vaccine production.
abstract: Affinity chromatography using sulfated, spherical cellulose beads (Cellufine Sulfate) was assessed for purification of influenza A and influenza B viruses. Recovery rates of viruses eluted from the beads were high for all tested virus strains. This method was also useful for removing chicken egg-derived impurities from allantoic fluids containing influenza viruses; the hemagglutination activity per amount of protein in the eluted sample was significantly higher than that in the applied sample. These results suggest that use of Cellufine Sulfate is a practical method for primary purification of influenza viruses in the process of influenza vaccine production.
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Okamatsu
authors.forename: Norikazu
authors.initials: N
authors.lastname: Isoda
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Koichi
authors.initials: K
authors.lastname: Ozaki
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Umeda
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Aoyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
mesh_terms: D000327:Adsorption; D000818:Animals; D001717:Birds; D002642:Chick Embryo; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009980:Influenza A virus / Q000737:chemistry / Q000302:isolation & purification*; D009981:Influenza B virus / Q000737:chemistry / Q000302:isolation & purification*; D007252:Influenza Vaccines / Q000737:chemistry / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000821:virology*; D007251:Influenza, Human / Q000821:virology*; D011108:Polymers / Q000737:chemistry; D011201:Poultry Diseases / Q000821:virology*
chemical_list: D007252:Influenza Vaccines; D011108:Polymers; C080965:sulfate cellufine