title: Antioxidant effect of Azadirachta indica on high fat diet induced diabetic Charles Foster rats.
abstract: Oxidative stress plays a major role in the pathogenesis of both types of diabetes mellitus. Excessively high levels of free radicals cause damage to cellular proteins, membrane lipids and nucleic acids, and eventually cell death. The present study was designed to investigate the possible effect of Azadirachta indica leaf extract in high fat diet induced diabetic Charles Foster rats. The increased level of lipidperoxidation and altered levels of enzymatic (superoxide dismutase, glutathione peroxidase and catalase) and non-enzymatic (glutathione) antioxidants were seen in high fructose fed animals. The treatment with A. indica leaf extract significantly normalized the altered levels of lipid peroxidation and antioxidant status at 400 mg/kg b.w. dose. The A. indica leaf extract was also tested for in vitro inhibition of generation of superoxide anion and hydroxyl free radical in both enzymatic and non-enzymatic systems. The A. indica leaf extract was found to inhibit generation of superoxide anion and hydroxyl free radical significantly at 200 μg/ml concentration. Data of present study demonstrated that the A. indica leaf extract has both antidiabetic and antioxidant properties.
authors.affiliation: Biochemistry Division, Central Drug Research Institute, 1 M. G. Marg, Lucknow 226001, India. cdri.atul@gmail.com
authors.forename: Atul
authors.initials: A
authors.lastname: Shrivastava
authors.forename: Upma
authors.initials: U
authors.lastname: Chaturvedi
authors.forename: Ravi
authors.initials: R
authors.lastname: Sonkar
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Khanna
authors.forename: J K
authors.initials: JK
authors.lastname: Saxena
authors.forename: Gitika
authors.initials: G
authors.lastname: Bhatia
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D031601:Azadirachta / Q000737:chemistry*; D003920:Diabetes Mellitus / Q000188:drug therapy* / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D004195:Disease Models, Animal; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats
chemical_list: D000975:Antioxidants; D010936:Plant Extracts