title: Spectroscopic analyses on sonocatalytic damage to bovine serum albumin (BSA) induced by ZnO/hydroxylapatite (ZnO/HA) composite under ultrasonic irradiation.
abstract: ZnO/hydroxylapatite (ZnO/HA) composite with HA molar content of 6.0% was prepared by the method of precipitation and heat-treated at 500°C for 40min and was characterized by powder X-ray diffraction (XRD). The sonocatalytic activities of ZnO/HA composite was carried out through the damage of bovine serum albumin (BSA) in aqueous solution. Furthermore, the effects of several factors on the damage of BSA molecules were evaluated by means of UV-vis and fluorescence spectra. Experimental results indicated that the damage degree of BSA aggravated with the increase of ultrasonic irradiation time, irradiation power and ZnO/HA addition amount, but weakened with the increase of solution acidity and ionic strength. In addition, the damage site to BSA was also studied by synchronous fluorescence technology and the damage site was mainly at tryptophan (Trp) residue. This paper provides a valuable reference for driving sonocatalytic method to treat tumor in clinic application.
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Zhiqiu
authors.initials: Z
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Mingming
authors.initials: M
authors.lastname: Zou
authors.forename: Jingqun
authors.initials: J
authors.lastname: Gao
authors.forename: Yumei
authors.initials: Y
authors.lastname: Kong
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Guangxi
authors.initials: G
authors.lastname: Han
mesh_terms: D000818:Animals; D002384:Catalysis / Q000528:radiation effects; D002417:Cattle; D017886:Durapatite / Q000737:chemistry*; D006358:Hot Temperature; D011208:Powders; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000528:radiation effects*; D012965:Sodium Chloride / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014465:Ultrasonics / Q000379:methods*; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D011208:Powders; D012996:Solutions; D012710:Serum Albumin, Bovine; D012965:Sodium Chloride; D017886:Durapatite; D015034:Zinc Oxide