title: A role for anti-CD45RB monoclonal antibody treatment upon dendritic cells.
abstract: Selective interference with CD45RB isoform by monoclonal antibody (anti-CD45RBmAb) reliably induces donor-specific tolerance. Dendritic cells (DCs) are the most potent antigen-presenting cells that are capable of activating naïve T cells. The purposes of the present study were to investigate the roles of anti-CD45RBmAb on the phenotypes and functioning of DCs and to further illustrate the mechanism of anti-CD45RBmAb-inducing immunologic tolerance. DCs from C57BL/6 mice were cultured and treated with various doses of anti-CD45RB monoclonal antibody. Cell phenotype, cycle and phagocytic ability were detected by flow cytometry. The production of IL-10 and IL-12 in the supernatants of mature DCs was measured with ELISA. Exosomes (Dex) were recovered from the supernatant of DCs cultured for 6 days in depleted medium, and effects of DCs and Dex on the ability of T-cell proliferation were detected by mixed lymphocyte culture. Anti-CD45RBmAb could inhibit DCs maturation in a dose-dependent manner, and the effects of exosomes (Dex) on DCs enhance or inhibition proliferation of T cells were also in a dose-dependent manner. Anti-CD45RBmAb could profoundly inhibit the maturation and functioning of DCs and generate tolerogenic dendritic cells (tDCs) as well as Dex, suggesting mechanistic contributions to tolerance development from the DCs through interactions with T cells.
authors.affiliation: Clinical Medical Research Center, The Second Clinical Medical College, Shenzhen People's Hospital, Ji'nan University, No. 1017 Dongmen North Road, Shenzhen, 518020, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Qi
authors.forename: Jin-Peng
authors.initials: JP
authors.lastname: Liu
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Deng
authors.forename: Han-Xin
authors.initials: HX
authors.lastname: Zhou
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Deng
authors.forename: Fu-Rong
authors.initials: FR
authors.lastname: Li
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000276:immunology / Q000494:pharmacology*; D002450:Cell Communication / Q000187:drug effects / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D007108:Immune Tolerance / Q000187:drug effects* / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000037:antagonists & inhibitors / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; C556062:IL10 protein, mouse; D016753:Interleukin-10; D018664:Interleukin-12; D017493:Leukocyte Common Antigens