title: [Effects of AZT on leukemia cell line KG-1a proliferation and telomerase activity].
abstract: This study was purposed to investigate the effect of 3'-azido-2', 3'-dideoxythymidine (AZT)on the proliferation and telomerase activity of human acute myeloid leukemia cell line KG-1a. The effect of proliferation was detected by MTT assay after the KG-1a cell were stimulated for 24, 48 and 72 h with different concentrations of AZT; telomerase activity was detected with TRAP-PCR-ELISA assay; RT-PCR was used to detect telomerase hTERT mRNA expression. The results showed that the proliferation of KG-1a cells was inhibited in a time and concentration dependent manner after exposure to AZT for 24, 48 and 72 h; the KG-1a cells decreased in S phase and increased in G(2)/M phase with the increasing of the concentration of AZT; telomerase activity and hTERT-mRNA expression in the experimental groups decreased after treated with AZT, which was positively correlated with concentration of AZT. It is concluded that AZT inhibits KG-1a cell proliferation and induces apoptosis, which maybe related with its decreasing the telomerase activity and hTERT mRNA expression.
authors.affiliation: Department of Hematology, The First Clinical Hospital of Lanzhou University, Lanzhou, China.
authors.forename: Rui-Rui
authors.initials: RR
authors.lastname: Jin
authors.forename: Rong
authors.initials: R
authors.lastname: Chao
authors.forename: Ya-Ming
authors.initials: YM
authors.lastname: Xi
authors.forename: Che
authors.initials: C
authors.lastname: Chen
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Chu
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015215:Zidovudine / Q000494:pharmacology*
chemical_list: D015215:Zidovudine; D019098:Telomerase