title: Phycicoccus ochangensis sp. nov., isolated from soil of a potato cultivation field.
abstract: Two novel, Gram-positive, motile, coccal bacteria, strains L1b-b9(T) and B5a-b5, were isolated from a potato cultivation field in Ochang, Korea. These isolates grew at 10-45°C, pH 5.0-10.0, and in the presence of 8% (w/v) NaCl. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major menaquinone was MK-8(H(4)) and the main cellular fatty acids were iso-C(14:0), iso-C(15:0), and anteiso-C(15:0). Polar lipids in strain L1b-b9(T) consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and an unknown glyco-amino lipid. The G+C content of genomic DNA was 73.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains L1b-b9(T) and B5a-b5 shared 99.36% similarity and formed a robust clade with the type species of the genus Phycicoccus. Strain L1b-b9(T) is related most closely to Phycicoccus cremeus V2M29(T) (97.52% 16S rRNA gene sequence similarity). On the basis of phylogenetic characteristics, the name Phycicoccus ochangensis sp. nov. is proposed for strain LIb-b9(T) (=KCTC 19695(T) [corrected] =JCM 17595(T)).
authors.affiliation: Microbiological Resource Center, KRIBB, Daejeon, 305-806, Republic of Korea.
authors.forename: Hyangmi
authors.initials: H
authors.lastname: Kim
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Oh
authors.forename: Doo-Sang
authors.initials: DS
authors.lastname: Park
authors.forename: Kang Hyun
authors.initials: KH
authors.lastname: Lee
authors.forename: Sung Uk
authors.initials: SU
authors.lastname: Kim
authors.forename: Hee-Moon
authors.initials: HM
authors.lastname: Park
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Bae
mesh_terms: D000192:Actinomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D011198:Solanum tuberosum / Q000254:growth & development / Q000382:microbiology
chemical_list: D005227:Fatty Acids; D012965:Sodium Chloride