title: A new lupane-type triterpene from the seeds of Panax ginseng with its inhibition of NF-κB.
abstract: A new lupane-triterpene, 3β-cis-feruloyloxy-16β-hydroxylup-20(29)-ene (1) were isolated from the ethyl acetate extract of Panax ginseng seeds along with the known compound, 3β-transferuloyloxy-16β-hydroxylup-20(29)-ene (2). Compound 2 was isolated from this plant for the first time. Their chemical structures were determined by mass spectroscopy and one-dimensional and two-dimensional magnetic resonance spectra. The bioactive effects of these compounds on TNF-α-induced NF-κB transcription were evaluated in transfected HepG2 cells. Effects on the expression of NF-κB target genes were also examined using a reverse transcription-polymerase chain reaction. Both compounds 1 and 2 were inhibited NF-κB activity in HepG2 cells by decreasing the cellular concentrations of inflammatory factors iNOS and COX2.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 305-764, Korea.
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Son
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Yang
authors.forename: Seok Bean
authors.initials: SB
authors.lastname: Song
authors.forename: Gyu Yong
authors.initials: GY
authors.lastname: Song
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D005894:Panax / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014162:Transfection; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D053978:Pentacyclic Triterpenes
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D051546:Cyclooxygenase 2; D016328:NF-kappa B; D052247:Nitric Oxide Synthase Type II; D014315:Triterpenes; C480546:lupane; D053978:Pentacyclic Triterpenes; C496319:NOS2 protein, human