title: Cerium oxide nanoparticles protect cardiac progenitor cells from oxidative stress.
abstract: Cardiac progenitor cells (CPCs) are a promising autologous source of cells for cardiac regenerative medicine. However, CPC culture in vitro requires the presence of microenvironmental conditions (a complex array of bioactive substance concentration, mechanostructural factors, and physicochemical factors) closely mimicking the natural cell surrounding in vivo, including the capability to uphold reactive oxygen species (ROS) within physiological levels in vitro. Cerium oxide nanoparticles (nanoceria) are redox-active and could represent a potent tool to control the oxidative stress in isolated CPCs. Here, we report that 24 h exposure to 5, 10, and 50 μg/mL of nanoceria did not affect cell growth and function in cardiac progenitor cells, while being able to protect CPCs from H(2)O(2)-induced cytotoxicity for at least 7 days, indicating that nanoceria in an effective antioxidant. Therefore, these findings confirm the great potential of nanoceria for controlling ROS-induced cell damage.
authors.affiliation: Laboratory of Cellular and Molecular Cardiology, Department of Internal Medicine, University of Rome Tor Vergata, Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Pagliari
authors.forename: Corrado
authors.initials: C
authors.lastname: Mandoli
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Forte
authors.forename: Eugenio
authors.initials: E
authors.lastname: Magnani
authors.forename: Stefania
authors.initials: S
authors.lastname: Pagliari
authors.forename: Giorgia
authors.initials: G
authors.lastname: Nardone
authors.forename: Silvia
authors.initials: S
authors.lastname: Licoccia
authors.forename: Marilena
authors.initials: M
authors.lastname: Minieri
authors.forename: Paolo
authors.initials: P
authors.lastname: Di Nardo
authors.forename: Enrico
authors.initials: E
authors.lastname: Traversa
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002563:Cerium / Q000737:chemistry*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D009206:Myocardium / Q000166:cytology*; D053758:Nanoparticles; D018384:Oxidative Stress; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D002563:Cerium; C030583:ceric oxide