title: The distribution of a germline methylation marker suggests a regional mechanism of LINE-1 silencing by the piRNA-PIWI system.
abstract: BACKGROUND
  A defense system against transposon activity in the human germline based on PIWI proteins and piRNA has recently been discovered. It represses the activity of LINE-1 elements via DNA methylation by a largely unknown mechanism. Based on the dispersed distribution of clusters of piRNA genes in a strand-specific manner on all human chromosomes, we hypothesized that this system might work preferentially on local and proximal sequences. We tested this hypothesis with a methylation-associated SNP (mSNP) marker which is based on the density of C-T transitions in CpG dinucleotides as a surrogate marker for germline methylation.
  
  
  RESULTS
  We found significantly higher density of mSNPs flanking piRNA clusters in the human genome for flank sizes of 1-16 Mb. A dose-response relationship between number of piRNA genes and mSNP density was found for up to 16 Mb of flanking sequences. The chromosomal density of hypermethylated LINE-1 elements had a significant positive correlation with the chromosomal density of piRNA genes (r = 0.41, P = 0.05). Genome windows of 1-16 Mb containing piRNA clusters had significantly more hypermethylated LINE-1 elements than windows not containing piRNA clusters. Finally, the minimum distance to the next piRNA cluster was significantly shorter for hypermethylated LINE-1 compared to normally methylated elements (14.4 Mb vs 16.1 Mb).
  
  
  CONCLUSIONS
  Our observations support our hypothesis that the piRNA-PIWI system preferentially methylates sequences in close proximity to the piRNA clusters and perhaps physically adjacent sequences on other chromosomes. Furthermore they suggest that this proximity effect extends up to 16 Mb. This could be due to an unknown localization signal, transcription of piRNA genes near the nuclear membrane or the presence of an unknown RNA molecule that spreads across the chromosome and targets the methylation directed by the piRNA-PIWI complex. Our data suggest a region specific molecular mechanism which can be sought experimentally.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Iceland, Reykjavík IS-101, Iceland.
authors.forename: Martin I
authors.initials: MI
authors.lastname: Sigurdsson
authors.forename: Albert V
authors.initials: AV
authors.lastname: Smith
authors.forename: Hans T
authors.initials: HT
authors.lastname: Bjornsson
authors.forename: Jon J
authors.initials: JJ
authors.lastname: Jonsson
mesh_terms: D060565:Argonaute Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D019175:DNA Methylation; D004251:DNA Transposable Elements; D020868:Gene Silencing; D006801:Humans; D020084:Long Interspersed Nucleotide Elements; D020641:Polymorphism, Single Nucleotide; D034741:RNA, Small Interfering
chemical_list: D060565:Argonaute Proteins; D004251:DNA Transposable Elements; C421088:PIWIL1 protein, human; D034741:RNA, Small Interfering