title: Microsatellite markers for Grosmannia alacris (Ophiostomataceae, Ascomycota) and other species in the G. serpens complex.
abstract: OBJECTIVE
  Polymorphic microsatellite markers were developed for the pine-infecting fungus, Grosmannia alacris.
  
  
  RESULTS
  Sixteen microsatellite markers were developed by using inter-simple sequence repeat (ISSR)-PCRs and 454 sequencing methods. Seven of these markers showed polymorphisms for a South African population of G. alacris, and 13 markers showed polymorphism when European isolates were included. Most of the primer pairs also amplified four closely related species: G. serpens, Leptographium gibbsii, L. castellanum, and L. yamaokae.
  
  
  CONCLUSIONS
  These new markers will be useful for population studies of G. alacris and other species in the G. serpens complex.
authors.affiliation: Department of Genetics, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South Africa.
authors.forename: Tuan A
authors.initials: TA
authors.lastname: Duong
authors.forename: Z Wilhelm
authors.initials: ZW
authors.lastname: de Beer
authors.forename: James W
authors.initials: JW
authors.lastname: Zanzot
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Wingfield
authors.forename: Brenda D
authors.initials: BD
authors.lastname: Wingfield
mesh_terms: D056426:Genetic Loci / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D055155:Ophiostomatales / Q000235:genetics*; D013019:South Africa