title: Influence of fatty acids on mitochondrial metabolism of adipocyte progenitors and endothelial cells.
abstract: BACKGROUND
  In obesity, the cells are exposed to excessive amounts of nutrients, especially free fatty acids (FFAs) that induce a variety of metabolic changes.
  
  
  OBJECTIVE
  We investigated the effect of FFAs on the mitochondrial function in different cell populations under stress conditions.
  
  
  METHODS
  Human adipose tissue progenitor cells (SVF) or endothelial cells (HUVECs) were incubated with 30μM of selected saturated or unsaturated FFA for 24 h, at times supplemented with 5ng/mL tumour necrosis factor alpha (TNFα) for the last 4 h. Changes in oxygen respiration rate, mitochondrial membrane potential (mitoMP) and total ATP content were monitored.
  
  
  RESULTS
  Saturated palmitic acid demonstrated no effect, while a selection of unsaturated FFAs ameliorated metabolism of the progenitor SVF cells. TNFα either did not affect or nullified some of the favourable FFA-induced effects.
  
  
  CONCLUSIONS
  The mitoMP was the most sensitive parameter reflecting positive impact of the unsaturated FFA on the adipose SVF cells' metabolism.
authors.affiliation: Department of Clinical Biochemistry, Jagiellonian University Medical College, Krakow, Poland. amknapp@cm-uj.krakow.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Knapp
authors.forename: Urszula
authors.initials: U
authors.lastname: Czech
authors.forename: Joanna
authors.initials: J
authors.lastname: Góralska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sliwa
authors.forename: Anna
authors.initials: A
authors.lastname: Gruca
authors.forename: Beata
authors.initials: B
authors.lastname: Kieć-Wilk
authors.forename: Magdalena
authors.initials: M
authors.lastname: Awsiuk
authors.forename: Christoph
authors.initials: C
authors.lastname: Thiele
authors.forename: Wojciech
authors.initials: W
authors.lastname: Dudek
authors.forename: Aldona
authors.initials: A
authors.lastname: Dembińska-Kieć
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019069:Cell Respiration / Q000187:drug effects; D002478:Cells, Cultured; D005227:Fatty Acids / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D019308:Palmitic Acid / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D014409:Tumor Necrosis Factor-alpha; D019308:Palmitic Acid; D000255:Adenosine Triphosphate