title: An image analysis toolbox for high-throughput C. elegans assays.
abstract: We present a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and imaging systems. This WormToolbox is available through the open-source CellProfiler project and enables objective scoring of whole-worm high-throughput image-based assays of C. elegans for the study of diverse biological pathways that are relevant to human disease.
authors.affiliation: Imaging Platform, Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts, USA. carolina@broadinstitute.org
authors.forename: Carolina
authors.initials: C
authors.lastname: Wählby
authors.forename: Lee
authors.initials: L
authors.lastname: Kamentsky
authors.forename: Zihan H
authors.initials: ZH
authors.lastname: Liu
authors.forename: Tammy
authors.initials: T
authors.lastname: Riklin-Raviv
authors.forename: Annie L
authors.initials: AL
authors.lastname: Conery
authors.forename: Eyleen J
authors.initials: EJ
authors.lastname: O'Rourke
authors.forename: Katherine L
authors.initials: KL
authors.lastname: Sokolnicki
authors.forename: Orane
authors.initials: O
authors.lastname: Visvikis
authors.forename: Vebjorn
authors.initials: V
authors.lastname: Ljosa
authors.forename: Javier E
authors.initials: JE
authors.lastname: Irazoqui
authors.forename: Polina
authors.initials: P
authors.lastname: Golland
authors.forename: Gary
authors.initials: G
authors.lastname: Ruvkun
authors.forename: Frederick M
authors.initials: FM
authors.lastname: Ausubel
authors.forename: Anne E
authors.initials: AE
authors.lastname: Carpenter
mesh_terms: D000465:Algorithms; D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology*; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D010641:Phenotype; D012984:Software