title: Evaluation of diel patterns of relative changes in cell turgor of tomato plants using leaf patch clamp pressure probes.
abstract: Relative changes in cell turgor of leaves of well-watered tomato plants were evaluated using the leaf patch clamp pressure probe (LPCP) under dynamic greenhouse climate conditions. LPCP changes, a measure for relative changes in cell turgor, were monitored at three different heights of transpiring and non-transpiring leaves of tomato plants on sunny and cloudy days simultaneously with whole plant water uptake. Clear diel patterns were observed for relative changes of cell turgor of both transpiring and non-transpiring leaves, which were stronger on sunny days than on cloudy days. A clear effect of canopy height was also observed. Non-transpiring leaves showed relative changes in cell turgor that closely followed plant water uptake throughout the day. However, in the afternoon the relative changes of cell turgor of the transpiring leaves displayed a delayed response in comparison to plant water uptake. Subsequent recovery of cell turgor loss of transpiring leaves during the following night appeared insufficient, as the pre-dawn turgescent state similar to the previous night was not attained.
authors.affiliation: Wageningen UR Greenhouse Horticulture, P.O. Box 644, 6700AP Wageningen, The Netherlands.
authors.forename: Kang M
authors.initials: KM
authors.lastname: Lee
authors.forename: Steven M
authors.initials: SM
authors.lastname: Driever
authors.forename: Ep
authors.initials: E
authors.lastname: Heuvelink
authors.forename: Simon
authors.initials: S
authors.lastname: Rüger
authors.forename: Ulrich
authors.initials: U
authors.lastname: Zimmermann
authors.forename: Arie
authors.initials: A
authors.lastname: de Gelder
authors.forename: Leo F M
authors.initials: LF
authors.lastname: Marcelis
mesh_terms: D001692:Biological Transport; D001696:Biomechanical Phenomena; D018551:Solanum lycopersicum / Q000502:physiology* / Q000528:radiation effects; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods*; D059828:Plant Cells / Q000502:physiology*; D018515:Plant Leaves / Q000502:physiology* / Q000528:radiation effects; D018526:Plant Transpiration; D011312:Pressure; D013472:Sunlight; D013696:Temperature; D014867:Water / Q000502:physiology
chemical_list: D014867:Water