title: HSP DNAJB8 controls tumor-initiating ability in renal cancer stem-like cells.
abstract: Cancer stem-like cells (CSC) are a small population of cancer cells with superior tumor initiating, self-renewal, and differentiation properties. In this study, we show that the cancer-testis antigen and HSP40 family member DNAJB8 contributes to the CSC phenotype in renal cell carcinoma (RCC). DNAJB8 overexpression increased the percentage of side population (SP) cells representing CSCs in RCC cells, enhancing their tumor-initiating ability. Conversely, attenuation of DNAJB8 decreased SP cells and reduced tumor-initiating ability. The utility of DNAJB8 as an immunologic target was established in DNA vaccination experiments. Compared with immunization with the tumor-associated antigen survivin, which was expressed in both CSCs and non-CSCs in RCC, immunization with Dnajb8 expression plasmids yielded stronger antitumor effects. Together, our findings suggest that DNAJB8 plays a role in CSC maintenance and that it offers a candidate for CSC-targeting immunotherapy in RCC.
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hirohashi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Torigoe
authors.forename: Akari
authors.initials: A
authors.lastname: Takahashi
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Tamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Mori
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kanaseki
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Kamiguchi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Asanuma
authors.forename: Rena
authors.initials: R
authors.lastname: Morita
authors.forename: Alice
authors.initials: A
authors.lastname: Sokolovskaya
authors.forename: Junichi
authors.initials: J
authors.lastname: Matsuzaki
authors.forename: Ren
authors.initials: R
authors.lastname: Yamada
authors.forename: Reona
authors.initials: R
authors.lastname: Fujii
authors.forename: Harm H
authors.initials: HH
authors.lastname: Kampinga
authors.forename: Toru
authors.initials: T
authors.lastname: Kondo
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Isao
authors.initials: I
authors.lastname: Hara
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000502:physiology; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007114:Immunization; D007680:Kidney Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014411:Neoplastic Stem Cells / Q000502:physiology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D050956:HSP40 Heat-Shock Proteins