title: Identification and apoptotic potential of T-2 toxin metabolites in human cells.
abstract: The mycotoxin T-2 toxin, produced by various  Fusarium  species, is a widespread contaminant of grain and grain products. Knowledge about its toxicity and metabolism in the human body is crucial for any risk assessment as T-2 toxin can be detected in processed and unprocessed food samples. Cell culture studies using cells of human origin represent a potent model system to study the metabolic fate of T-2 toxin as well as the cytotoxicity in vitro. In this study the metabolism of T-2 toxin was analyzed in a cell line derived from human colon carcinoma cells (HT-29) and primary human renal proximal tubule epithelial cells (RPTEC) using high-performance liquid chromatography coupled with Fourier transformation mass spectrometry (HPLC-FTMS). Both cell types metabolized T-2 toxin to a variety of compounds. Furthermore, cell cycle analysis in RPTEC proved the apoptotic effect of T-2 toxin and its metabolites HT-2 toxin and neosolaniol in micromolar concentrations.
authors.affiliation: Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Münster, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Weidner
authors.forename: Tanja
authors.initials: T
authors.lastname: Welsch
authors.forename: Florian
authors.initials: F
authors.lastname: Hübner
authors.forename: Gerald
authors.initials: G
authors.lastname: Schwerdt
authors.forename: Michael
authors.initials: M
authors.lastname: Gekle
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Humpf
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle; D002478:Cells, Cultured; D002523:Edible Grain; D005506:Food Contamination; D005670:Fusarium / Q000378:metabolism*; D019073:HT29 Cells; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism; D013605:T-2 Toxin / Q000378:metabolism*
chemical_list: D013605:T-2 Toxin