title: A pentameric luminescent-conjugated oligothiophene for optical imaging of in vitro-formed amyloid fibrils and protein aggregates in tissue sections.
abstract: Luminescent-conjugated oligo- and polythiophenes (LCOs and LCPs) are valuable tools for optical imaging of a plethora of protein aggregates associated with amyloidoses. Here, we describe the utilization of an anionic pentameric LCO, p-FTAA, for staining of protein aggregates in a variety of platforms, including in vitro-formed amyloid fibrils and tissue sections.
authors.affiliation: Department of Chemistry, IFM, Linköping University, Linköping, Sweden. petni@ifm.liu.se
authors.forename: K Peter R
authors.initials: KP
authors.lastname: Nilsson
authors.forename: Mikael
authors.initials: M
authors.lastname: Lindgren
authors.forename: Per
authors.initials: P
authors.lastname: Hammarström
mesh_terms: D000085:Acetates / Q000378:metabolism; D000682:Amyloid / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology; D002417:Cattle; D004331:Drosophila melanogaster / Q000166:cytology; D005557:Formaldehyde / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D049408:Luminescent Agents / Q000378:metabolism*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D055620:Optical Phenomena; D016612:Paraffin Embedding; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011108:Polymers / Q000378:metabolism*; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013876:Thiophenes / Q000378:metabolism*; D016707:Tissue Fixation
chemical_list: D000085:Acetates; D000682:Amyloid; D016229:Amyloid beta-Peptides; D007328:Insulin; D049408:Luminescent Agents; D010446:Peptide Fragments; D011108:Polymers; D011994:Recombinant Proteins; D013876:Thiophenes; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42); C572917:pentamer formyl thiophene acetic acid; D005557:Formaldehyde; C066730:polythiophene