title: In vitro and in vivo identification of a novel cytotoxic T lymphocyte epitope from Rv3425 of Mycobacterium tuberculosis.
abstract: The identification of novel cytotoxic T lymphocyte (CTL) epitopes is important to analysis of the involvement of CD8(+) T cells in Mycobacterium tuberculosis infection as well as to the development of peptide vaccines. In this study, a novel CTL epitope from region of difference 11 encoded antigen Rv3425 was identified. Epitopes were predicted by the reversal immunology approach. Rv3425-p118 (LIASNVAGV) was identified as having relatively strong binding affinity and stability towards the HLA-A*0201 molecule. Peripheral blood mononuclear cells pulsed by this peptide were able to release interferon-γ in healthy donors (HLA-A*02(+) purified protein derivative(+)). In cytotoxicity assays in vitro and in vivo, Rv3425-p118 induced CTLs to specifically lyse the target cells. Therefore, this epitope could provide a subunit component for designing vaccines against Mycobacterium tuberculosis.
authors.affiliation: Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Ming-xia
authors.initials: MX
authors.lastname: Zhai
authors.forename: Yu-huang
authors.initials: YH
authors.lastname: Zhu
authors.forename: Yuan-ming
authors.initials: YM
authors.lastname: Qi
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Zhai
authors.forename: Yan-feng
authors.initials: YF
authors.lastname: Gao
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D019295:Computational Biology; D003601:Cytotoxicity Tests, Immunologic; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011485:Protein Binding; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D018984:Epitopes, T-Lymphocyte; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D007371:Interferon-gamma