title: Cloning and function analysis of a drought-inducible gene associated with resistance to Curvularia leaf spot in maize.
abstract: ZmDIP was cloned and its function against Curvularia lunata was analyzed, according to a previous finding on a drought-inducible protein in resistant maize identified through MALDI-TOF-MS/MS. The ZmDIP expression varied in roots, leaf sheaths, and young, as well as old, leaves of different maize inbred lines. The ZmDIP transcript level changed in leaves over the course of time after inoculation with C. lunata. A prokaryotic expression analysis demonstrated that the gene can regulate the salt stress tolerance of Escherichia coli. The ZmDIP transient expression in the maize leaf showed that the gene was also linked to leaf resistance against the C. lunata infection. ZmDIP-mediated ROS and ABA signaling pathways were inferred to be closely associated with maize leaf resistance to the pathogen infection.
authors.affiliation: Department of Plant Science, School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Jiewei
authors.initials: J
authors.lastname: Zhu
authors.forename: Xiuli
authors.initials: X
authors.lastname: Huang
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.forename: Shigang
authors.initials: S
authors.lastname: Gao
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D003001:Cloning, Molecular; D055864:Droughts; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007178:Inbreeding; D009928:Organ Specificity / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology*; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D003313:Zea mays / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins