title: Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts.
abstract: The aim of this study was to investigate the inhibitory activity of Gentiana lutea extracts on the enzyme myeloperoxidase (MPO), as well as the antioxidant activity of these extracts and their correlation with the total polyphenol content. Extracts were prepared using methanol (100%), water and ethanol aqueous solutions (96, 75, 50 and 25%v/v) as solvents for extraction. Also, isovitexin, amarogentin and gentiopicroside, pharmacologically active constituents of G. lutea were tested as potential inhibitors of MPO. Antioxidant activity of extracts was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and also using cyclic voltammetry (CV). Among all extracts, the antioxidant capacity of 50% ethanol aqueous extract was the highest, both when measured using the DPPH test, with IC(50)=20.6 μg/ml, and when using CV. Also, 50% ethanol extract, showed the best inhibition of MPO activity in comparison with other extracts. In the group of the selected G. lutea constituents, gentiopicroside has proved to be the strongest inhibitor of MPO, with IC(50)=0.8 μg/ml. Also, the concentration of G. lutea constituents were determined in all extracts, using Ultra Performance Liquid Chromatography (UPLC).
authors.affiliation: Department of Physical Chemistry, Vinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia. branislav@vinca.rs
authors.forename: Branislav
authors.initials: B
authors.lastname: Nastasijević
authors.forename: Tamara
authors.initials: T
authors.lastname: Lazarević-Pašti
authors.forename: Suzana
authors.initials: S
authors.lastname: Dimitrijević-Branković
authors.forename: Igor
authors.initials: I
authors.lastname: Pašti
authors.forename: Ana
authors.initials: A
authors.lastname: Vujačić
authors.forename: Gordana
authors.initials: G
authors.lastname: Joksić
authors.forename: Vesna
authors.initials: V
authors.lastname: Vasić
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D030012:Gentiana / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D009195:Peroxidase / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000302:isolation & purification / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D010936:Plant Extracts; D059808:Polyphenols; D012997:Solvents; D009195:Peroxidase