title: Taurine postponed the replicative senescence of rat bone marrow-derived multipotent stromal cells in vitro.
abstract: The aging of many mammalian tissues is associated with replicative decline in somatic stem cells. Postponing this decline is a direct way of anti-aging. Bone marrow-derived multipotent stromal cells (BMSCs) hold promise for an increasing list of therapeutic uses due to their multilineage potential. Clinical application of BMSCs requires abundant cells that can be overcome by ex vivo expansion of cells, but often facing the replicative senescence problem. We demonstrated that taurine exhibited anti-replicative senescence effect on rat BMSCs by promoting colony forming unit-fibroblast formation and cell proliferation, shortening cell population doubling time, enormously inhibiting senescence-associated beta-galactosidase activity and slowing the loss of differentiation potential, while having no significant effect on the maximum passage number and total culture time, and slight influences on the cell surface CD molecules expressions. Taurine is a quite safe antioxidant and nutrient extensively used in food addition and clinical treatment. These suggested that taurine is a promising anti-replicative senescence additive for ex vivo expansion of BMSCs in experimental and clinical cell therapies.
authors.affiliation: College of Life Science, ZheJiang University, ZiJinGang Campus, Room 325, Hangzhou 310058, China.
authors.forename: Huijiao
authors.initials: H
authors.lastname: Ji
authors.forename: Guiyun
authors.initials: G
authors.lastname: Zhao
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Luo
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects*; D008297:Male; D051381:Rats; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013654:Taurine / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D013654:Taurine; D001616:beta-Galactosidase