title: Stakelama sediminis sp. nov., isolated from tidal flat sediment.
abstract: A novel bacterial strain designated CJ70(T) was isolated from tidal flat sediment in Korea. A polyphasic approach was used to identify this strain taxonomically. The isolate was Gram-stain-negative, strictly aerobic, yellow-pigmented, rod-shaped and non-motile. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain CJ70(T) was related most closely to Stakelama pacifica JLT832(T) with 95.7 % similarity and formed an independent phyletic line from recognized species of the genus Sphingomonas, comprising a clade with Stakelama pacifica, which is the only recognized species of the genus Stakelama. The predominant cellular fatty acids of strain CJ70(T) were C(18 : 1)ω7c (60.0 %), C(16 : 0) (21.2 %) and C(14 : 0) 2-OH (5.8 %). The major isoprenoid quinone was ubiquinone-10. The G+C content of the genomic DNA was 61.4 mol%. The results obtained from this study suggested that strain CJ70(T) represents a novel species of the genus Stakelama, for which the name Stakelama sediminis sp. nov. is proposed. The type strain is CJ70(T) ( = KACC 16559(T) = JCM 18079(T)).
authors.affiliation: Department of Biotechnology (BK21 Program), Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: Cung Nawl
authors.initials: CN
authors.lastname: Thawng
authors.affiliation: Department of Biotechnology (BK21 Program), Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: Sung-Joon
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Biotechnology (BK21 Program), Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: Ju-Hee
authors.initials: JH
authors.lastname: Cha
authors.affiliation: Department of Biotechnology (BK21 Program), Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Cha
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2