title: Effect of chronic excess iodine intake on thyroid function and oxidative stress in hypothyroid rats.
abstract: Our objective was to investigate the effects of chronic excess iodine intake on thyroid functions and thyroid oxidative stress state in hypothyroid rats. Sixty rats were divided into euthyroid and hypothyroid (thiocyanate-induced) groups with or without administration of excess iodine (3000 or 6000 μg/L) for 8 weeks. Serum thyroxine (T(4)), triiodothyronine (T(3)), thyroid-stimulating hormone (TSH), thyroid antioxidants (catalase, superoxide dismutase enzymes, and total antioxidants), and lipid peroxide (malondialdehyde; MDA) were measured. Reverse transcription - PCR gene expression for thyroidal Na(+)/I(-) symporter (NIS), D1 deiodinase, and thyroid peroxidase (TPO) were performed. Thiocyanate significantly decreased thyroid hormones (T(3), T(4)), increased lipid peroxides and antioxidants, and increased gene expression of NIS, D1 deiodinase, and TPO. Excess iodine intake in hypothyroid rats increased T3 and T4. Also, high iodine intake by hypothyroid rats significantly decreased NIS, D1 deiodinase, and TPO genes expression. Excess iodine significantly increased MDA and antioxidants in euthyroid and hypothyroid rats. In conclusion, thiocyanate-hypothyroidism increases gene expression of NIS, TPO, and TPO and induces oxidative stress. High iodine intake decreases NIS and D1 deiodinase gene expression in hypothyroid rats. Moreover, excess iodine increase thyroid hormones, lipid peroxides, and antioxidants in hypothyroid rats.
authors.affiliation: Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt. zizomenna@yahoo.com
authors.forename: Abd El-Aziz M
authors.initials: Ael-A
authors.lastname: Hussein
authors.forename: Amr M
authors.initials: AM
authors.lastname: Abbas
authors.forename: Gehan A
authors.initials: GA
authors.lastname: El Wakil
authors.forename: Ayman Z
authors.initials: AZ
authors.lastname: Elsamanoudy
authors.forename: Azza Abd
authors.initials: AA
authors.lastname: El Aziz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D007037:Hypothyroidism / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D007453:Iodide Peroxidase / Q000235:genetics / Q000378:metabolism; D007455:Iodine / Q000008:administration & dosage* / Q000633:toxicity / Q000652:urine; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D027981:Symporters / Q000235:genetics / Q000378:metabolism; D013861:Thiocyanates / Q000378:metabolism; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism*; D013972:Thyrotropin / Q000097:blood / Q000378:metabolism; D013974:Thyroxine / Q000097:blood / Q000378:metabolism; D014284:Triiodothyronine / Q000097:blood / Q000378:metabolism; D000099209:Sodium-Iodide Symporters
chemical_list: D000975:Antioxidants; D002374:Catalase; D007453:Iodide Peroxidase; D007455:Iodine; D008054:Lipid Peroxides; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D027981:Symporters; D013861:Thiocyanates; D013972:Thyrotropin; D013974:Thyroxine; D014284:Triiodothyronine; D000099209:Sodium-Iodide Symporters; C031760:thiocyanate