title: In situ evaluation of the genotoxic potential of the river Nile: II. Detection of DNA strand-breakage and apoptosis in Oreochromis niloticus niloticus (Linnaeus, 1758) and Clarias gariepinus (Burchell, 1822).
abstract: This work is part of a wider eco-toxicological study proposed to evaluate the biological impact of contaminants along the whole course of the river Nile, Egypt. Here we present data on the presence of DNA strand-breaks and apoptotic cells assessed by use of comet and diffusion assays in erythrocytes of Nile tilapia (Oreochromis niloticus niloticus) and African catfish (Clarias gariepinus). The results showed high degrees of DNA damage and increased frequencies of apoptotic nuclei in blood of fish collected from downstream compared with those sampled from upstream river Nile. Qualitative analysis revealed a shift in the frequency of DNA-damage classes towards higher damage levels correlating with the increasing pollution gradient. The degree of DNA damage measured by use of comet assay and diffusion assay exhibited seasonal variations. Both fish species showed significant increases in DNA damage during the summer. The results of our study indicated that the alkaline comet assay seems to be a useful technique for in situ genotoxic monitoring. At the same time the diffusion assay is sensitive enough to detect low frequencies of apoptotic nuclei. The results reveal species-specific differences in sensitivities, suggesting that Nile tilapia may serve as a more sensitive test species compared with the African catfish. Based on the outcome of the comet and diffusion assays, it can be concluded that the water quality of the river Nile with respect to the presence of genotoxic compounds needs to be improved, especially in its estuaries. As far as we know this is the first time that the comet and diffusion assays are used for genotoxic monitoring of the river Nile.
authors.affiliation: Department of Zoology, Faculty of Science, Al-Azhar University (Assiut Branch), 71524 Assiut, Egypt; Department of Eco-physiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, 12587 Berlin, Germany. Electronic address: osman@igb-berlin.de.
authors.forename: Alaa G M
authors.initials: AGM
authors.lastname: Osman
authors.affiliation: The Egyptian Environmental Affairs Agency (EEAA), Assiut, Egypt.
authors.forename: Khaled Y
authors.initials: KY
authors.lastname: Abuel-Fadl
authors.affiliation: Department of Eco-physiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, 12587 Berlin, Germany; Department of Endocrinology, Humboldt University, Berlin, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Kloas
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002397:Catfishes / Q000235:genetics; D023681:Cichlids / Q000235:genetics; D020552:Comet Assay; D053960:DNA Breaks / Q000187:drug effects*; D004534:Egypt; D004784:Environmental Monitoring / Q000379:methods*; D009152:Mutagenicity Tests / Q000379:methods; D045483:Rivers; D012621:Seasons; D013045:Species Specificity; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical