title: Increased vertical dimension effects on masseter muscle fiber phenotype during maturation.
abstract: OBJECTIVE
  To determine changes in mouse myosin heavy chain (MyHC) protein expression that may occur with a clinically relevant vertical dimension of occlusion (VDO) increase.
  
  
  METHODS
  Six CD-1 male mice (age: 6 weeks) underwent a 10% bite opening to replicate the clinical condition using composite on the maxillary molars and were compared to six age-matched controls. Mice were sacrificed at day 7 and 14 after bite opening. A representative masseter transverse cryosection from each animal was examined in selected sampling regions (anterior, posterior, posterior-deep, and posterior-intermediate) to assay fiber phenotype proportions and fiber size.
  
  
  RESULTS
  In control masseter muscles, the proportion of muscle fibers containing MyHC IIb increased in the posterior-intermediate and posterior-deep regions between 7 and 14 days (ANOVA, P < .05). The increase in the proportion of MyHC IIb fibers in the bite opening group did not occur when compared to the control group (P < .05). In addition, after 14 days of bite opening, the proportion of fibers positive for MyHC IIa was decreased in the anterior region compared to control masseter muscles. Muscle fiber diameter remained unchanged in both groups (experimental and control) and over time (P > .10).
  
  
  CONCLUSIONS
  These data are consistent with a selective plasticity of the expression of MyHC IIb protein in the deep regions of the male masseter muscle in response to a clinically relevant VDO increase.
authors.affiliation: Department of Orthodontics, University of Florida College of Dentistry, Gainesville, FL 32610-0444, USA. widmer@dental.ufl.edu
authors.forename: Charles
authors.initials: C
authors.lastname: Widmer
authors.forename: Vo Danh
authors.initials: VD
authors.lastname: Nguyen
authors.forename: Harmeet
authors.initials: H
authors.lastname: Chiang
authors.forename: Joyce
authors.initials: J
authors.lastname: Morris-Wiman
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015870:Gene Expression; D008297:Male; D008406:Masseter Muscle / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D024343:Open Bite / Q000378:metabolism*; D010641:Phenotype; D014716:Vertical Dimension
chemical_list: D018995:Myosin Heavy Chains