title: [Susceptibility of Aedes albopictus and Culex pipiens quinquefasciatus to infection with bat Japanese encephalitis virus isolates].
abstract: OBJECTIVE
  To evaluate the susceptibility of Aedes albopictus and Culex pipiens quinquefasciatus to oral infection with bat Japanese encephalitis virus isolates (GD1 and HN2 strains).
  
  
  METHODS
  Aedes albopictus and Culex pipiens quinquefasciatus were infected orally by GD1 and HN2 strains of bat Japanese encephalitis virus. TaqMan real-time PCR was used to detect the virus and monitor the changes in the viral loads in Aedes albopictus and Culex pipiens quinquefasciatus at a 2-day interval, starting from 4 days till 20 days after the infection.
  
  
  RESULTS
  The infected Aedes albopictus and Culex pipiens quinquefasciatus were found positive for the Japanese encephalitis virus from day 4 to day 20. Both Aedes albopictus and Culex pipiens quinquefasciatus were susceptible to infection by GD1 and HN2 strains, but the latter showed a greater susceptibility. The HN2 strain virus appeared to have a greater virulence than the GD1 strain.
  
  
  CONCLUSIONS
  Aedes albopictus and Culex pipiens quinquefasciatus can carry GD1 and HN2 strains of bat Japanese encephalitis virus isolates.
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. graystar92@gmail.com
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.forename: Qionghua
authors.initials: Q
authors.lastname: Zhang
authors.forename: Junhua
authors.initials: J
authors.lastname: Zhou
authors.forename: Shouyi
authors.initials: S
authors.lastname: Yu
authors.forename: Xueli
authors.initials: X
authors.lastname: Zheng
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D002685:Chiroptera / Q000821:virology*; D003465:Culex / Q000821:virology*; D004198:Disease Susceptibility; D004664:Encephalitis Virus, Japanese / Q000302:isolation & purification*