title: Development of polymorphic microsatellite markers in Camellia chekiangoleosa (Theaceae) using 454-ESTs.
abstract: OBJECTIVE
  A set of microsatellite markers for Camellia chekiangoleosa was developed and characterized using 454 sequencing technology to study the population genetic structure and the diversity of germplasm collections.
  
  
  RESULTS
  Eighteen polymorphic microsatellite markers were identified and tested in 150 individuals from three natural populations of C. chekiangoleosa. Alleles numbered from two to seven, and the observed and expected heterozygosities ranged from 0.100 to 0.760 and 0.133 to 0.809, respectively.
  
  
  CONCLUSIONS
  These markers will potentially be conducive to further genetic studies on C. chekiangoleosa.
authors.affiliation: The Key Laboratory of Forest Genetics and Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wen
authors.forename: Linchu
authors.initials: L
authors.lastname: Xu
authors.forename: Yincong
authors.initials: Y
authors.lastname: Gu
authors.forename: Minren
authors.initials: M
authors.lastname: Huang
authors.forename: Lian
authors.initials: L
authors.lastname: Xu
mesh_terms: D028244:Camellia / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism; D020224:Expressed Sequence Tags; D056426:Genetic Loci / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D017931:DNA Primers