title: Metabonomic responses in rat urine following subacute exposure to propoxur.
abstract: Metabolic profiling of urine from pesticide-treated rats was investigated by the nuclear magnetic resonance (NMR)-based metabonomic strategy. Twenty-four-hour urine samples of rats were collected after administration with propoxur at doses of 0.85, 1.70, and 8.51 mg/kg, respectively, for 28 consecutive days. Liver tissue was fixed and the histopathological alterations were examined. The results showed that propoxur at high dose induced liver histopathological injury. Metabonomic analysis demonstrated that the levels of creatine and taurine markedly increased together with slight elevation of hippurate, glucose, and amino acids in low- and medium-dose groups. However, concentrations of urinary lactate, acetate, acetone, succinate, citrate, and 2-oxoglutarate increased in high-dose group. All these results suggested that propoxur could inhibit liver function through altering the energy and lipid metabolism. These data also supported the contention that the NMR-based metabonomic approach represents a promising new technology for the development of pesticide toxicity screening and mechanism exploration.
authors.affiliation: Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Beijing, People’s Republic of China.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Liang
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Wu
mesh_terms: D000818:Animals; D002264:Carboxylic Acids / Q000652:urine; D003401:Creatine / Q000652:urine; D005998:Glycine / Q000652:urine; D007306:Insecticides / Q000493:pharmacokinetics / Q000633:toxicity* / Q000652:urine*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics; D001074:Propoxur / Q000493:pharmacokinetics / Q000633:toxicity* / Q000652:urine*; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000652:urine
chemical_list: D002264:Carboxylic Acids; D007306:Insecticides; D013654:Taurine; D001074:Propoxur; D003401:Creatine; D005998:Glycine