title: Single-cell enzymatic dissociation of hESC lines OxF1-OxF4 and culture in feeder-free conditions.
abstract: Experimental manipulation of hESCs has been hampered by their fragility and susceptibility to apoptosis when dissociated into single cells. The OxF lines are particularly robust and may be successfully passaged as single cells, with the inclusion of ROCK inhibitor in the medium. The protocols here describe the enzymatic dissociation of hESCs into a single-cell suspension and the plating of these cells onto either feeder cells or a protein-coated surface.
authors.affiliation: Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Women's Centre, John Radcliffe Hospital, Headington, Oxford, UK. frances.brook@obs-gyn.ox.ac.uk
authors.forename: Frances A
authors.initials: FA
authors.lastname: Brook
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology*; D004798:Enzymes / Q000378:metabolism*; D061252:Feeder Cells / Q000166:cytology; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D019096:Vitronectin / Q000378:metabolism
chemical_list: D004798:Enzymes; D005353:Fibronectins; D019096:Vitronectin