title: Synthesis and characterization of a paramagnetic sialic acid conjugate as probe for magnetic resonance applications.
abstract: Magnetic Resonance Imaging (MRI) using paramagnetic systems as contrast agents is receiving increased attention as diagnostic tool in the clinic. At the same time, NMR of paramagnetic systems can also be applied in biochemical fields; for example, the use of Paramagnetic Relaxation Enhancement (PRE) allows structure refinement and the analysis of transient dynamic processes involved in macromolecular complex formation. Herein we report the synthesis and computational characterization of a new DOTA-like sialic acid conjugate, which can be used both in MRI and PRE applications when coordinated to a suitable paramagnetic metal.
authors.affiliation: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Bini
authors.forename: Maria
authors.initials: M
authors.lastname: Gregori
authors.forename: Ugo
authors.initials: U
authors.lastname: Cosentino
authors.forename: Giorgio
authors.initials: G
authors.lastname: Moro
authors.forename: Angeles
authors.initials: A
authors.lastname: Canales
authors.forename: Alice
authors.initials: A
authors.lastname: Capitoli
authors.forename: Jesús
authors.initials: J
authors.lastname: Jiménez-Barbero
authors.forename: Laura
authors.initials: L
authors.lastname: Cipolla
mesh_terms: D003287:Contrast Media / Q000138:chemical synthesis / Q000737:chemistry*; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012794:Sialic Acids / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D006573:Heterocyclic Compounds, 1-Ring; D009942:Organometallic Compounds; D012794:Sialic Acids; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid