title: Biological activity of SE-10, granulated powder of Sasa senanensis Rehder leaf extract.
abstract: BACKGROUND
  We have previously reported that alkaline extract of Sasa senanensis leaves (SE) showed potent anti-HIV, anti-UV and radical scavenging activity. In the present study, we investigated the biological activities of SE-10, a granulated powder of SE supplemented with lactose, lactitol, trehalose and tea extract.
  
  
  METHODS
  Cell viability of mock-infected, HIV-infected, and UV-irradiated cells was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Scavenging activity of superoxide anion and hydroxyl radicals was determined by electron-spin resonance spectroscopy. Cytochrome P-450 (CYP)3A4 activity was measured by β-hydroxylation of testosterone in human recombinant CYP3A4.
  
  
  RESULTS
  SE-10 had slightly higher anti-HIV and anti-UV activities, but slightly lower radical-scavenging and CYP3A4-inhibitory activities, as compared with SE.
  
  
  CONCLUSIONS
  The present study demonstrates that the biological activities of SE were well preserved during the manufacturing process of SE-10.
authors.affiliation: Division of Pharmacology, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan. sakagami@dent.meikai.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakagami
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Matsuta
authors.forename: Kazue
authors.initials: K
authors.lastname: Satoh
authors.forename: Sumiko
authors.initials: S
authors.lastname: Ohtsuki
authors.forename: Chiyako
authors.initials: C
authors.lastname: Shimada
authors.forename: Taisei
authors.initials: T
authors.lastname: Kanamoto
authors.forename: Shigemi
authors.initials: S
authors.lastname: Terakubo
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakashima
authors.forename: Yurika
authors.initials: Y
authors.lastname: Morita
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Tadashi
authors.initials: T
authors.lastname: Tsuda
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Sunaga
authors.forename: Jun
authors.initials: J
authors.lastname: Maki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sugiura
authors.forename: Madoka
authors.initials: M
authors.lastname: Kitajima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Oizumi
authors.forename: Takaaki
authors.initials: T
authors.lastname: Oizumi
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D016166:Free Radical Scavengers / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D031787:Sasa / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D019380:Anti-HIV Agents; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011837:Radiation-Protective Agents