title: Co-localization of hippocampal cholinergic neurostimulating peptide precursor with collapsin response mediator protein-2 at presynaptic terminals in hippocampus.
abstract: Hippocampal cholinergic neurostimulating peptide (HCNP) induces the synthesis of acetylcholine in medial septal nucleus in vitro and in vivo. HCNP precursor protein (HCNP-pp) is a multifunctional protein that participates in a number of signaling pathways, including MAPK/extracellular signal and G-protein-coupled receptor kinase 2. We recently demonstrated that the amount of collapsin response mediator protein-2 (CRMP-2) is increased in hippocampus of HCNP-pp transgenic mice. To clarify the interaction between HCNP/HCNP-pp and CRMP-2 and its role in synaptic function, we investigated whether HCNP-pp is localized to the synapse and if it affects protein expression. Here, we demonstrate that HCNP-pp co-localizes with CRMP-2 at presynaptic terminals. Furthermore, HCNP-pp overexpression increases synaptophysin levels. These findings suggest that HCNP-pp, in association with CRMP-2, plays an important role in presynaptic function in the hippocampus.
authors.affiliation: Department of Neurology, Nagoya City University, 1 Kawasumi, Nagoya 467-8601, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kato
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Mitake
authors.forename: Masayuki
authors.initials: M
authors.lastname: Mizuno
authors.forename: Tetsuko
authors.initials: T
authors.lastname: Kanamori
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kosei
authors.initials: K
authors.lastname: Ojika
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Matsukawa
mesh_terms: D000818:Animals; D015153:Blotting, Western; D000076122:Disks Large Homolog 4 Protein; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016253:Microscopy, Immunoelectron; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000378:metabolism; D016708:Synaptophysin / Q000096:biosynthesis
chemical_list: D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D016708:Synaptophysin; C095114:collapsin response mediator protein-2; C075274:hippocampal cholinergic neurostimulating peptide