title: An improved medium for growing Staphylococcus aureus biofilm.
abstract: A medium (Brain Heart Infusion plus 10% human plasma) was developed, tested, and validated for growing Staphylococcus aureus biofilm in vitro. With this medium, S. aureus forms reproducible and robust biofilms in flow chambers under controlled shear flow and with increased viability recovery in static well plates.
authors.affiliation: Microbiology Branch, US Army Dental and Trauma Research Detachment, Institute of Surgical Research, Fort Sam Houston, TX 78234, USA. ping.chen@us.army.mil
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Johnathan J
authors.initials: JJ
authors.lastname: Abercrombie
authors.forename: Nicole R
authors.initials: NR
authors.lastname: Jeffrey
authors.forename: Kai P
authors.initials: KP
authors.lastname: Leung
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D018441:Biofilms / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D050296:Microbial Viability; D010949:Plasma / Q000378:metabolism; D013211:Staphylococcus aureus / Q000254:growth & development / Q000502:physiology*
chemical_list: D003470:Culture Media