title: Phosphorylated modification and in vitro antioxidant activity of Radix Hedysari polysaccharide.
abstract: Phosphorylated modification of a polysaccharide obtained from Radix Hedysari (RHP) was studied. Three phosphorylated polysaccharides (RHPP) with variable degrees of substitution (DS(p)) were obtained with 4-dimethylaminopyridine (DMAP) and N, N' Dicyclocarbodiimide (DCC) as catalyst. The structures of RHPP were characterized by FT-IR spectra and (13)C NMR spectra. Depending on different reaction time, RHPP showed different DS(p) ranging from 0.30 to 0.66, and different Mw ranging from 86.6 to 89.7 KDa. Compared with RHP, RHPP exhibited superior antioxidant activities in vitro, which indicated that phosphorylated modification could enhance antioxidant activities of RHP. Furthermore, it was obvious that the DS(p) had a significant effect on the antioxidant activity.
authors.affiliation: School of Basic Medical Science, Lanzhou University, Lanzhou, 730000, China. weidf07@lzu.edu.cn
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Wei
authors.forename: Weidong
authors.initials: W
authors.lastname: Cheng
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Wei
authors.forename: Lifeng
authors.initials: L
authors.lastname: Zhang
mesh_terms: D015761:4-Aminopyridine / Q000031:analogs & derivatives / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D010084:Oxidation-Reduction; D010766:Phosphorylation; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D029626:Ranunculaceae / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D011134:Polysaccharides; D017665:Hydroxyl Radical; D015761:4-Aminopyridine; C003885:4-dimethylaminopyridine