title: A novel Th cell epitope of Candida albicans mediates protection from fungal infection.
abstract: Fungal pathogens are a frequent cause of opportunistic infections. They live as commensals in healthy individuals but can cause disease when the immune status of the host is altered. T lymphocytes play a critical role in pathogen control. However, specific Ags determining the activation and function of antifungal T cells remain largely unknown. By using an immunoproteomic approach, we have identified for the first time, to our knowledge, a natural T cell epitope from Candida albicans. Isolation and sequencing of MHC class II-bound ligands from infected dendritic cells revealed a peptide that was recognized by a major population of all Candida-specific Th cells isolated from infected mice. Importantly, human Th cells also responded to stimulation with the peptide in an HLA-dependent manner but without restriction to any particular HLA class II allele. Immunization of mice with the peptide resulted in a population of epitope-specific Th cells that reacted not only with C. albicans but also with other clinically highly relevant species of Candida including the distantly related Candida glabrata. The extent of the reaction to different Candida species correlated with their degree of phylogenetic relationship to C. albicans. Finally, we show that the newly identified peptide acts as an efficient vaccine when used in combination with an adjuvant inducing IL-17A secretion from peptide-specific T cells. Immunized mice were protected from fatal candidiasis. Together, these results uncover a new immune determinant of the host response against Candida ssp. that could be exploited for the development of antifungal vaccines and immunotherapies.
authors.affiliation: Institute of Microbiology, Swiss Federal Institute of Technology Zürich, 8093 Zürich, Switzerland.
authors.forename: Eva
authors.initials: E
authors.lastname: Bär
authors.forename: André
authors.initials: A
authors.lastname: Gladiator
authors.forename: Sonia
authors.initials: S
authors.lastname: Bastidas
authors.forename: Bernd
authors.initials: B
authors.lastname: Roschitzki
authors.forename: Hans
authors.initials: H
authors.lastname: Acha-Orbea
authors.forename: Annette
authors.initials: A
authors.lastname: Oxenius
authors.forename: Salomé
authors.initials: S
authors.lastname: LeibundGut-Landmann
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology / Q000517:prevention & control*; D002460:Cell Line; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005657:Fungal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000382:microbiology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
chemical_list: D018984:Epitopes, T-Lymphocyte; D005657:Fungal Vaccines; D022223:Vaccines, Subunit