title: Labdane-type diterpenoids from hairy root cultures of Coleus forskohlii, possible intermediates in the biosynthesis of forskolin.
abstract: Significant attention has been devoted to studying hairy root cultures as a promising strategy for production of various valuable secondary metabolites. These offer many advantages, such as high growth rate, genetic stability and being hormone-free. In this study, a detailed phytochemical investigation of the secondary metabolites of Coleus forskohlii hairy root cultures was undertaken and which resulted in the isolation of 22 compounds, including four forskolin derivatives and a monoterpene. Their structures were elucidated by extensive spectroscopic analyses. These compounds could be classified into four groups viz.: labdane-type diterpenes, monoterpenes, triterpenes and phenylpropanoid dimers. Apart from one compound, all labdane type diterpenes are oxygenated at C-11 as in forskolin and a scheme showing their biosynthetic relationships is proposed.
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. asaday@rs.noda.tus.ac.jp
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Asada
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Terada
authors.forename: Xinzhu
authors.initials: X
authors.lastname: Kuang
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Takafumi
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Shogo
authors.initials: S
authors.lastname: Hamaguchi
authors.forename: Iyuki
authors.initials: I
authors.lastname: Namekata
authors.forename: Hikaru
authors.initials: H
authors.lastname: Tanaka
authors.forename: Kazuo
authors.initials: K
authors.lastname: Koike
mesh_terms: D031330:Coleus / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D005576:Colforsin / Q000031:analogs & derivatives / Q000378:metabolism*; D046508:Culture Techniques; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D039821:Monoterpenes / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004224:Diterpenes; D039821:Monoterpenes; C433017:labdane; D005576:Colforsin