title: Novosphingobium barchaimii sp. nov., isolated from hexachlorocyclohexane-contaminated soil.
abstract: A yellow-pigmented bacterial strain, designated LL02(T), was isolated from hexachlorocyclohexane-contaminated soil from Spolana Neratovice, a former Czech producer of lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL02(T) occupied a distinct phylogenetic position in the genus Novosphingobium and showed the highest sequence similarity with Novosphingobium resinovorum NCIMB 8767(T) (98.59 %). DNA-DNA relatedness between strain LL02(T) and its closest phylogenetic neighbours was <70 %, which indicated that strain LL02(T) represented a novel species of the genus Novosphingobium. The DNA G+C content of strain LL02(T) was 67.72±0 mol%. The major respiratory quinone was ubiquinone Q-10. The polar lipid profile of the isolate corresponded to those reported for other members of the genus Novosphingobium (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine and sphingoglycolipids), thus supporting its classification in the genus. Spermidine was the major polyamine. The major fatty acids were summed feature 3 (consisting of C(16 : 1)ω7c and/or C(16 : 1)ω6c; 40.13 %), summed feature 8 (consisting of C(18 : 1)ω7c and/or C(18 : 1)ω6c; 31.09 %) and C(14 : 0) 2-OH (23.16 %). The results obtained from DNA-DNA hybridization and biochemical and physiological tests clearly distinguished the isolate from its closest phylogenetic neighbours. Thus, strain LL02(T) represents a novel species of the genus Novosphingobium, for which the name Novosphingobium barchaimii sp. nov. is proposed. The type strain is LL02(T) ( = CCM 7980(T)  = DSM 25411(T)).
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Niharika
authors.affiliation: International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Moskalikova
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
authors.forename: Jasvinder
authors.initials: J
authors.lastname: Kaur
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Sedlackova
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic.|International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Hampl
authors.affiliation: Loschmidt Laboratories and Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, 628 00 Brno, Czech Republic.|International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Damborsky
authors.affiliation: Loschmidt Laboratories and Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, 628 00 Brno, Czech Republic.
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Prokop
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
authors.forename: Rup
authors.initials: R
authors.lastname: Lal
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D001556:Hexachlorocyclohexane; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D013095:Spermidine / Q000032:analysis; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D014451:Ubiquinone; D001556:Hexachlorocyclohexane; C024989:coenzyme Q10; D013095:Spermidine