title: Resistance to Bombyx mori nucleopolyhedrovirus via overexpression of an endogenous antiviral gene in transgenic silkworms.
abstract: Transgenic technology is a powerful tool for improving disease-resistant species. Bmlipase-1, purified from the midgut juice of Bombyx mori, showed strong antiviral activity against B. mori nucleopolyhedrovirus (BmNPV). In an attempt to create an antiviral silkworm strain for sericulture, a transgenic vector overexpressing the Bmlipase-1 gene was constructed under the control of a baculoviral immediate early-1 (IE1) promoter. Transgenic lines were generated via embryo microinjection. The mRNA level of Bmlipase-1 in the midguts of the transgenic line was 27.3 % higher than that of the non-transgenic line. After feeding the silkworm with different amounts of BmNPV, the mortality of the transgenic line decreased to approximately 33 % compared with the non-transgenic line when the virus dose was 10(6) OB/larva. These results imply that overexpressing endogenous antiviral genes can enhance the antiviral resistance of silkworms.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Jiang
authors.forename: Genhong
authors.initials: G
authors.lastname: Wang
authors.forename: Tingcai
authors.initials: T
authors.lastname: Cheng
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
authors.forename: Shengkai
authors.initials: S
authors.lastname: Jin
authors.forename: Gai
authors.initials: G
authors.lastname: Lu
authors.forename: Fuquan
authors.initials: F
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiao
authors.forename: Hanfu
authors.initials: H
authors.lastname: Xu
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D012831:Bombyx / Q000201:enzymology / Q000235:genetics* / Q000821:virology*; D015971:Gene Expression Regulation, Enzymologic; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000201:enzymology / Q000235:genetics / Q000821:virology; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D017924:Nucleopolyhedroviruses / Q000502:physiology*
chemical_list: D019476:Insect Proteins; D008049:Lipase