title: Determination of tryptophan in raw materials, rat brain and human plasma by RP-HPLC technique.
abstract: This paper describes tryptophan (TRP) estimation in raw human plasma and rat brain by reversed-phase high-performance liquid chromatography (RP-HPLC). Estimation was carried out on a Purospher STAR C18 column using water-acetonitrile (90:10 v/v, at pH 2.7) mixture at a rate of 1.5 mL/min as mobile phase. Eluents were monitored at 273 nm by an ultraviolet detector. The method was linear (R(2) > 0.999), precise (intra-day and inter-day precision <2%) in the range of 0.25-20 µg/mL. The detection and quantification limits were 0.0144 µg/mL and 0.0437 µg/mL, respectively. In human plasma, Day 1 and Day 2 precision were 0.054-2.29% and 1.66-3.7%; whereas precisions in rat brain were 1.23-2.3% and 0.677-4.2%, respectively. The method was applied to study TRP level in human smokers and in arthritic rat brain. An efficient RP-HPLC method was developed for TRP determination that worked for clinical and research purposes.
authors.affiliation: United Biotechnologies, Gulistan-e-Jauhar, 75290 Karachi, Pakistan.
authors.forename: Najma
authors.initials: N
authors.lastname: Sultana
authors.forename: M Saeed
authors.initials: MS
authors.lastname: Arayne
authors.forename: Moona Mehboob
authors.initials: MM
authors.lastname: Khan
authors.forename: Darakhshan M
authors.initials: DM
authors.lastname: Saleem
authors.forename: Agha Zeeshan
authors.initials: AZ
authors.lastname: Mirza
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014364:Tryptophan / Q000032:analysis* / Q000097:blood
chemical_list: D014364:Tryptophan