title: Influence of protic ionic liquids on the structure and stability of succinylated Con A.
abstract: We report the synthesis of a series of ionic liquids (ILs) from various ions having different kosmotropicity including dihydrogen phosphate (H(2)PO(4)(-)), hydrogen sulfate (HSO(4)(-)) and acetate (CH(3)COO(-)) as anions and chaotropic cation such as trialkylammonium cation. To characterize the biomolecular interactions of ILs with protein, we have explored the stability of succinylated Con A (S Con A) in the presence of these aqueous ILs, which are varied combinations of kosmotropic anion with chaotropic cation such as triethylammonium dihydrogen phosphate [(CH(3)CH(2))(3)NH][H(2)PO(4)] (TEAP), trimethylammonium acetate [(CH(3))(3)NH][CH(3)COO] (TMAA), trimethylammonium dihydrogen phosphate [(CH(3))(3)NH][H(2)PO(4)] (TMAP) and trimethylammonium hydrogen sulfate [(CH(3))(3)NH][HSO(4)] (TMAS). Circular dichroism (CD) and fluorescence experiments have been used to characterize the stability of S Con A by ILs. Our data distinctly demonstrate that the long alkyl chain IL TEAP is a strong stabilizer for S Con A. Further, our experimental results reveal that TEAP is an effective refolding enhancer for S Con A from a thermally denatured protein structure.
authors.affiliation: Department of Chemistry, University of Delhi, Delhi, India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Attri
authors.forename: Pannuru
authors.initials: P
authors.lastname: Venkatesu
mesh_terms: D003208:Concanavalin A / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D011489:Protein Denaturation; D058849:Protein Refolding; D055550:Protein Stability; D013816:Thermodynamics
chemical_list: D052578:Ionic Liquids; D003208:Concanavalin A; C019558:succinylconcanavalin A