title: Involvement of protein kinase C in reduction of aggregated protein and phosphorylation of CREB in glomeruli.
abstract: We previously demonstrated the cAMP-PKA pathway to be associated with the reduction in aggregated proteins such as immune complex in glomeruli. The aim of this study was to clarify whether PKC is involved in the reduction of aggregated protein and phosphorylation of CREB in aggregated protein-loaded glomeruli. Mice were injected with aggregated bovine serum albumin (a-BSA), and glomeruli were isolated. The a-BSA-injected mice produced more cyclic AMP and had more phosphorylated serine and phosphorylated CREB in their glomeruli than the controls. The expression of phospho-CREB increased with the accumulation of a-BSA. KT5720 and H7 suppressed the increase in phosphorylated CREB in a-BSA-loaded glomeruli and the decrease in accumulated a-BSA in the glomeruli. These findings suggest that PKC is associated with the reduction of aggregated protein and phosphorylation of CREB in aggregated protein-loaded glomeruli.
authors.affiliation: Laboratory of Pharmacobiology and Therapeutics, Faculty of Pharmacy, Meijo University, Nagoya 468-8503, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hirasawa
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nishiyama
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Nagao
authors.forename: Yibin
authors.initials: Y
authors.lastname: Feng
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nagamatsu
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000818:Animals; D050882:CREB-Binding Protein / Q000378:metabolism*; D002227:Carbazoles / Q000494:pharmacology; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007678:Kidney Glomerulus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010085:Oxidative Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011758:Pyrroles / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000378:metabolism / Q000494:pharmacology
chemical_list: D002227:Carbazoles; D004791:Enzyme Inhibitors; D011758:Pyrroles; D012710:Serum Albumin, Bovine; C057416:KT 5720; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000242:Cyclic AMP; D050882:CREB-Binding Protein; C494364:Crebbp protein, mouse; D011493:Protein Kinase C