title: The interaction of HspA1A with TLR2 and TLR4 in the response of neutrophils induced by ovarian cancer cells in vitro.
abstract: Inducible heat shock protein (HspA1A) promotes tumor cell growth and survival. It also interacts with effector cells of the innate immune system and affects their activity. Recently, we showed that the direct contact of ovarian cancer cells, isolated from tumor specimens, with neutrophils intensified their biological functions. Our current experiments demonstrate that the activation of neutrophils, followed by an increased production of reactive oxygen species, by cancer cells involves the interaction of HspA1A from cancer cells with Toll-like receptors 2 and 4 expressed on the neutrophils' surface. Our data may have a practical implication for targeted anticancer therapies based, among other factors, on the inhibition of HspA1A expression in the cancer cells.
authors.affiliation: Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, Lodz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Klink
authors.forename: Marek
authors.initials: M
authors.lastname: Nowak
authors.forename: Michał
authors.initials: M
authors.lastname: Kielbik
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Bednarska
authors.forename: Edyta
authors.initials: E
authors.lastname: Blus
authors.forename: Marian
authors.initials: M
authors.lastname: Szpakowski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szyllo
authors.forename: Zofia
authors.initials: Z
authors.lastname: Sulowska
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D018920:Coculture Techniques; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D018840:HSP70 Heat-Shock Proteins; C490786:HSPA1A protein, human; D017382:Reactive Oxygen Species; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4