title: Improved semen collection method for wild felids: urethral catheterization yields high sperm quality in African lions (Panthera leo).
abstract: For wild and domestic felids, electroejaculation (EE) is the most common semen collection method. However, the equipment is expensive, there is a risk of urine contamination and animals usually show strong muscular contraction despite general anesthesia. Accordingly, we tested the feasibility of a different approach using urethral catheterization (UC) in seven African lions, previously described for domestic cats only. After general anesthesia with the α2-agonist medetomidine (which also stimulates semen release into the urethra) and ketamine, a transrectal ultrasound was performed to locate the prostate. A commercial dog urinary catheter (2.6 or 3.3 mm in diameter) was advanced approximately 30 cm into the urethra to allow semen collection into the lumen of the catheter by capillary forces. After retraction, sperm volumes between of 422.86 ± 296.07 μl yielded motility of 88.83 ± 13.27% (mean ± SD) with a mean sperm concentration of 1.94 × 10(9)/ml. Here we describe a simple, field friendly and effective method to attain highly concentrated semen samples with excellent motility in lions and potentially other wild felid species as an alternative to electroejaculation.
authors.affiliation: Geolifes-Animal Fertility and Reproductive Research, Frohmestr 7, 22 457 Hamburg, Germany. imke.lueders@gmail.com
authors.forename: I
authors.initials: I
authors.lastname: Lueders
authors.forename: I
authors.initials: I
authors.lastname: Luther
authors.forename: G
authors.initials: G
authors.lastname: Scheepers
authors.forename: G
authors.initials: G
authors.lastname: van der Horst
mesh_terms: D000768:Anesthesia, General / Q000662:veterinary; D000818:Animals; D004542:Ejaculation; D004558:Electric Stimulation; D008045:Lions; D008297:Male; D033401:Microscopy, Acoustic / Q000662:veterinary; D012661:Semen; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility; D020858:Tissue and Organ Harvesting / Q000379:methods / Q000662:veterinary*; D014546:Urinary Catheterization / Q000295:instrumentation / Q000662:veterinary