title: Abnormal expression pattern of the ASPP family of proteins in human             non-small cell lung cancer and regulatory functions on apoptosis through p53 by             iASPP.
abstract: The p53 protein is one of the best-known tumor suppressors. Recently discovered             ASPP1 and ASPP2 are specific activators and iASPP is an inhibitor of p53. In the             present study, we found that of 37 NSCLC patients, p53 alterations were detected             in 20 tumors (54.1%), the mRNA expression of ASPP1 and ASPP2 was frequently dowregulated             in tumor tissues, and this decreased significantly in samples expressing wild-type             p53. The expression of ASPP1 and ASPP2 was downregulated and that of iASPP was             upregulated in two NSCLC cell lines (the NCI-H157 cell line with altered p53 and             the A549 cell line with wild-type p53). The NCI-H157 cell with higher ASPP1 and             ASPP2 levels was more sensitive to cisplatin than the A549 cells with lower ASPP1             and ASPP2 levels. Downregulation of iASPP by siRNA stimulated apoptosis through             p53 in two NSCLC cell lines. These findings provide new insights into the molecular             mechanisms of action of the ASPP family in NSCLC and may have potent therapeutic             applications.
authors.affiliation: Department of Clinical Laboratory, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, PR China. lishijun@dl.cn
authors.forename: Shijun
authors.initials: S
authors.lastname: Li
authors.forename: Guangxia
authors.initials: G
authors.lastname: Shi
authors.forename: Hong
authors.initials: H
authors.lastname: Yuan
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D015536:Down-Regulation; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D017385:Sequence Homology; D014158:Transcription, Genetic / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; C498406:PPP1R13B protein, human; C119612:PPP1R13L protein, human; D012097:Repressor Proteins; C495901:TP53 protein, human; C090468:TP53BP2 protein, human; D016159:Tumor Suppressor Protein p53; D002945:Cisplatin