title: Structural and ultrastructural study of the rabbit testes exposed to carbamate insecticide.
abstract: The present study was conducted to investigate the impact of carbamate insecticide - bendiocarb on the testicular structure of adult rabbits. Bendiocarb was perorally administered daily for 10 and 30 days, at a dose 5 mg/kg of body weight. After the histological sampling the tissues were investigated and compared with control. After the bendiocarb administration the absolute and relative testicular weight decreased significantly (P < 0.001) in both time periods. The testicular parenchyma showed structural changes such the sloughing of developing sex cells, occurrence of vacuoles within Sertoli cells and inside various spermatogenic cells. The interstitial Leydig cells were smaller than their control counterpart and possessed shrivelled nuclei and strongly vacuolar dark cytoplasm. The rate of changes was directly proportional on duration of the experiment. The ultrastructural examination proved presence of various cellular defects across the germinal epithelium as well as within the interstitial Leydig cells in both experimental periods. Morphometric analysis manifested decrease in diameters of seminiferous tubules, increase of the diameters of the tubular lumina due to reduction of height of the seminiferous epithelium. Results of this study show distinct negative effects of bendiocarb on structure of rabbit testes.
authors.affiliation: Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic. almasiova@uvlf.sk
authors.forename: Viera
authors.initials: V
authors.lastname: Almasiova
authors.forename: Katarina
authors.initials: K
authors.lastname: Holovska
authors.forename: Lucia
authors.initials: L
authors.lastname: Tarabova
authors.forename: Viera
authors.initials: V
authors.lastname: Cigankova
authors.forename: Agnesa
authors.initials: A
authors.lastname: Lukacinova
authors.forename: Frantisek
authors.initials: F
authors.lastname: Nistiar
mesh_terms: D000818:Animals; D002219:Carbamates / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D011817:Rabbits; D013737:Testis / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D002219:Carbamates; D007306:Insecticides