title: Synthesis, characterization, and antimicrobial activity of nano-hydroxyapatite-zinc for bone tissue engineering applications.
abstract: The bone implants used in tissue repair are susceptible to infections caused by staphylococci, specifically Staphylococcus aureus. Hence, the development of better biological materials that provide antimicrobial activity in bone tissue engineering is required. The nanoparticles of hydroxyapatite (nHAp) and nHAp dopped with Zn (nHAp-Zn) were prepared by the wet chemical method and the ion exchange method, respectively. They were characterized using SEM, AFM, FTIR and XRD. The antibacterial activity of nHAp and nHAp-Zn was determined with Gram-negative and Gram-positive bacterial strains. The results indicated that nHAp alone was acting as an inert matrix and when substituted with Zn, it showed better antibacterial activity. The nHAp-Zn was found to be non-toxic to osteoprogenitor cells. Thus, due to the antimicrobial property of nHAp-Zn nanoparticles, we suggest that they would have potential applications towards bone tissue engineering.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Swetha
authors.forename: K
authors.initials: K
authors.lastname: Sahithi
authors.forename: A
authors.initials: A
authors.lastname: Moorthi
authors.forename: N
authors.initials: N
authors.lastname: Saranya
authors.forename: S
authors.initials: S
authors.lastname: Saravanan
authors.forename: K
authors.initials: K
authors.lastname: Ramasamy
authors.forename: N
authors.initials: N
authors.lastname: Srinivasan
authors.forename: N
authors.initials: N
authors.lastname: Selvamurugan
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D049329:Nanostructures / Q000008:administration & dosage* / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects*; D010316:Particle Size; D051381:Rats; D023822:Tissue Engineering / Q000379:methods; D015032:Zinc / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D018786:Bone Substitutes; D017886:Durapatite; D015032:Zinc