title: Comparative analysis of three methods for HPV DNA detection in cervical samples.
abstract: High risk HPV infection is considered to play a central role in cervical carcinogenesis. HPV DNA testing has shown to be a very useful tool for screening and following cervical infections. The aim of this study was to compare three methods for HPV DNA detection, along with cytology and colposcopy analysis. Cervical samples were collected from 100 sexually active women in Mérida, western Venezuela. HPV infection was screened using Hybrid-Capture 2 (HC2), L1-Nested-PCR and E6/E7-PCR assays. 40% of the samples (40/100) were HPV positive by at least one of the DNA detection methods. HC2 detected HPV in 12% specimens. L1- and E6/E7-PCRs showed 50% sensitivity and 77% specificity.The agreement rate between HC2 and both PCR assays was 65%. Kappa value showed moderate concordance between HC2 and both PCR methods (kappa=0.55; CI 95%). Also moderate concordance was seen when L1- and E6/E7-PCRs were compared (kappa=0.48; CI 95%). There was a significant association between the Schiller test and E6/E7-PCR (p=0.006) for HPV infection. An acceptable agreement between all three assays for HPV detection was observed. Nevertheless, different PCR formats need to be further analyzed in order to make the right choice of method for HPV testing.
authors.affiliation: Laboratorio de Microbiología y Salud Pública del Estado Mérida, Universidad de Los Andes, Mérida, Venezuela.
authors.forename: Elvia
authors.initials: E
authors.lastname: Michelli
authors.forename: Luis
authors.initials: L
authors.lastname: Téllez
authors.forename: José-Andrés
authors.initials: JA
authors.lastname: Mendoza
authors.forename: Claudia
authors.initials: C
authors.lastname: Jürgensen
authors.forename: Maritza
authors.initials: M
authors.lastname: Muñoz
authors.forename: Saberio
authors.initials: S
authors.lastname: Pérez
authors.forename: Noraida
authors.initials: N
authors.lastname: Mosqueda
authors.forename: Erick
authors.initials: E
authors.lastname: Hernández
authors.forename: María-Eugenia
authors.initials: ME
authors.lastname: Noguera
authors.forename: Diana
authors.initials: D
authors.lastname: Callejas
authors.forename: María
authors.initials: M
authors.lastname: Correnti
authors.forename: María-Eugenia
authors.initials: ME
authors.lastname: Cavazza
authors.forename: Silvana
authors.initials: S
authors.lastname: Vielma
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D002584:Cervix Uteri / Q000821:virology*; D003127:Colposcopy; D016384:Consensus Sequence; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D016679:Genome, Viral; D006801:Humans; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D009856:Oncogene Proteins, Viral / Q000235:genetics; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology; D002575:Uterine Cervicitis / Q000473:pathology / Q000821:virology; D014626:Vaginal Smears
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral