title: Mitochondrial lysyl-tRNA synthetase independent import of tRNA lysine into yeast mitochondria.
abstract: Aminoacyl tRNA synthetases play a central role in protein synthesis by charging tRNAs with amino acids. Yeast mitochondrial lysyl tRNA synthetase (Msk1), in addition to the aminoacylation of mitochondrial tRNA, also functions as a chaperone to facilitate the import of cytosolic lysyl tRNA. In this report, we show that human mitochondrial Kars (lysyl tRNA synthetase) can complement the growth defect associated with the loss of yeast Msk1 and can additionally facilitate the in vitro import of tRNA into mitochondria. Surprisingly, the import of lysyl tRNA can occur independent of Msk1 in vivo. This suggests that an alternative mechanism is present for the import of lysyl tRNA in yeast.
authors.affiliation: Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, Andhra Pradesh, India. nareshuohyd@gmail.com
authors.forename: Naresh Babu V
authors.initials: NB
authors.lastname: Sepuri
authors.forename: Madhavi
authors.initials: M
authors.lastname: Gorla
authors.forename: Michael P
authors.initials: MP
authors.lastname: King
mesh_terms: D006801:Humans; D008250:Lysine-tRNA Ligase / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology*; D012357:RNA, Transfer, Lys / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D038744:Ribosomal Protein S6 Kinases, 90-kDa / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D012357:RNA, Transfer, Lys; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D038744:Ribosomal Protein S6 Kinases, 90-kDa; C116919:mitogen and stress-activated protein kinase 1; D008250:Lysine-tRNA Ligase