title: Serum concentrations and tissue expression of components of insulin-like growth factor-axis in females with type 2 diabetes mellitus and obesity: the influence of very-low-calorie diet.
abstract: We explored serum concentrations and mRNA expression of insulin-like-growth factor-1 (IGF-1) axis components in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM) of 18 healthy females, 11 obese non-diabetic females (OB) and 13 obese women with type 2 diabetes (T2DM) examined at baseline and after very-low-calorie diet (VLCD). T2DM women had decreased expression of IGF-1, IGF-1 receptor (IGF-1R), IGFBP-2 (IGF binding protein-2) and IGFBP-3 in SCAT and increased expression of IGF-1R in PM compared to control group. IGF-1R and IGFBP-3 mRNA expression in SCAT of OB was comparable to control group. In T2DM women VLCD increased serum levels and SCAT expression of IGFBP-2 and PM expression of IGFBP-3. We conclude that decreased IGF-1, IGF-1R and IGFBP-3 expression in SCAT and increased IGF-1R expression in PM of T2DM subjects might contribute to changes of fat differentiation capacity and to regulation of subclinical inflammation by PM, respectively. Increased SCAT and circulating IGFBP-2 and IGFBP-3 in PM might participate in metabolic improvements after VLCD.
authors.affiliation: Third Department of Medicine, General University Hospital and First Faculty of Medicine, Charles University, Prague 2, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Touskova
authors.forename: P
authors.initials: P
authors.lastname: Trachta
authors.forename: P
authors.initials: P
authors.lastname: Kavalkova
authors.forename: J
authors.initials: J
authors.lastname: Drapalova
authors.forename: D
authors.initials: D
authors.lastname: Haluzikova
authors.forename: M
authors.initials: M
authors.lastname: Mraz
authors.forename: Z
authors.initials: Z
authors.lastname: Lacinova
authors.forename: J
authors.initials: J
authors.lastname: Marek
authors.forename: M
authors.initials: M
authors.lastname: Haluzik
mesh_terms: D031204:Caloric Restriction; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications* / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006728:Hormones / Q000097:blood; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D009765:Obesity / Q000097:blood* / Q000150:complications* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D050151:Subcutaneous Fat / Q000378:metabolism
chemical_list: D006728:Hormones; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I