title: Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates.
abstract: Amino acid sequence variations have possible influences on the allergenicity of allergens and may be important factors in allergen standardization. This study was undertaken to investigate the sequence polymorphisms of group 1 and 2 allergens from Korean isolates of the house dust mites Dermatophagoides farinae and D. pteronyssinus. cDNA sequences encoding group 1 and 2 allergens were amplified by RT-PCR and compared the deduced amino acid sequences. Der f 1.0101, which appeared in 64.0 % of the 50 sequences analyzed, was found to be predominant. Among the Der p 1 sequences, Der p 1.0102 and 1.0105 were predominant (58 %). Among the Der f 2 sequences, Der f 2.0102 (40.7 %) and a new variant with Gly at position 42 (27.8 %) were predominant. The deduced amino acid sequences of 60 Der p 2 clones were examined, and 28 variants with 1-5 amino acid substitutions were found. Interestingly, all of the Der p 2 sequences had Thr instead of Lys at position 49. Two variants (Leu40, Thr49, and Asn114 (26.6 %); Val40, Thr49, and Asn114 (20.0 %)) were found to be the most predominant forms of Der p 2. Der p 1 has a high rate of sporadic substitutions and the group 2 allergens show a more regular pattern with orderly associations of amino acid substitutions. Der f 1 and Der p 2 from Korean mite isolates have unique amino acid sequence polymorphisms. These findings provide important data for house dust mite allergen standardization.
authors.affiliation: Department of Internal Medicine and Institute of Allergy, Yonsei University College of Medicine, 50 Yonei-ro, Seodaemun-gu, Seoul, 120-752, Korea. jeongky@yuhs.ac
authors.forename: Kyoung Yong
authors.initials: KY
authors.lastname: Jeong
authors.forename: In-Yong
authors.initials: IY
authors.lastname: Lee
authors.forename: Tai-Soon
authors.initials: TS
authors.lastname: Yong
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Kim
authors.forename: Joo-Shil
authors.initials: JS
authors.lastname: Lee
authors.forename: Chein-Soo
authors.initials: CS
authors.lastname: Hong
authors.forename: Jung-Won
authors.initials: JW
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D060829:Arthropod Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D040001:Dermatophagoides farinae / Q000378:metabolism*; D040002:Dermatophagoides pteronyssinus / Q000378:metabolism*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D056910:Republic of Korea
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451160:Dermatophagoides farinae antigen f 2; C451163:Dermatophagoides pteronyssinus antigen p 2; D003546:Cysteine Endopeptidases; C451159:Dermatophagoides farinae antigen f 1; C451162:Dermatophagoides pteronyssinus antigen p 1