title: Separation and purification of furanocoumarins from Toddalia asiatica (L.) Lam. using microwave-assisted extraction coupled with high-speed counter-current chromatography.
abstract: A method of microwave-assisted extraction coupled with high-speed counter-current chromatography was established for separation and purification of isopimpinellin, pimpinellin and phellopterin from Toddalia asiatica (L.) Lam. The conditions of MAE including the extraction solvent, size of sample, solid/liquid ratio, extraction temperature and extraction time were optimized by a mono-factor test. That is, 2.0 g dried powder of T. asiatica (L.) Lam of 0.30-0.15 mm size was extracted with 20 mL (solid/liquid ratio of 1:10, g/mL) methanol under 50 °C for 1 min. The crude extract was separated and purified by high-speed counter-current chromatography with hexane-ethyl acetate-methanol-water (5:5:5.5:4.5, v/v/v/v) solvent system. 0.85 mg/g of isopimpinellin, 2.55 mg/g of pimpinellin and 0.95 mg/g of phellopterin were obtained from original sample in one-step within 240 min, the purity determined by high performance liquid chromatography was 95.0%, 99.1% and 96.4%, respectively. Their chemical structures were further identified by mass spectroscopy and nuclear magnetic resonance spectroscopy. The results demonstrated that microwave-assisted extraction coupled with high-speed counter-current chromatography was a feasible, economical and efficient technique for rapid extraction, separation and purification of effective compounds from natural products.
authors.affiliation: Department of Chemistry, Hanshan Normal University, Chaozhou, P. R. China.
authors.forename: Heyuan
authors.initials: H
authors.lastname: Qiu
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Xiao
authors.forename: Gongke
authors.initials: G
authors.lastname: Li
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D011564:Furocoumarins / Q000032:analysis / Q000302:isolation & purification*; D008872:Microwaves; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D027821:Rutaceae / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D011564:Furocoumarins; D010936:Plant Extracts