title: Secretory pathway research: the more experimental systems the better.
abstract: Transient gene expression, in plant protoplasts or specific plant tissues, is a key technique in plant molecular cell biology, aimed at exploring gene products and their modifications to examine functional subdomains, their interactions with other biomolecules, and their subcellular localization. Here, we highlight some of the major advantages and potential pitfalls of the most commonly used transient gene expression models and illustrate how ectopic expression and the use of dominant mutants can provide insights into protein function.
authors.affiliation: Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom.
authors.forename: Jurgen
authors.initials: J
authors.lastname: Denecke
authors.forename: Fernando
authors.initials: F
authors.lastname: Aniento
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Frigerio
authors.forename: Chris
authors.initials: C
authors.lastname: Hawes
authors.forename: Inhwan
authors.initials: I
authors.lastname: Hwang
authors.forename: Jaideep
authors.initials: J
authors.lastname: Mathur
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Neuhaus
authors.forename: David G
authors.initials: DG
authors.lastname: Robinson
mesh_terms: D001692:Biological Transport; D005456:Fluorescent Dyes / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D011523:Protoplasts / Q000378:metabolism / Q000648:ultrastructure; D012106:Research; D055571:Secretory Pathway
chemical_list: D005456:Fluorescent Dyes