title: Sperm DNA fragmentation index does not correlate with the sperm or embryo aneuploidy rate in recurrent miscarriage or implantation failure patients.
abstract: BACKGROUND
  The aneuploidy rate is higher in poor-quality sperm samples, which also have higher DNA fragmentation index values. The aim of this study was to assess the relationship between sperm DNA fragmentation in samples from infertile men belonging to couples with recurrent miscarriage or implantation failure and the aneuploidy rate in spermatozoa as well as in embryos from patients.
  
  
  METHODS
  This prospective study evaluated DNA damage and the aneuploidy rate in fresh and processed (density gradient centrifugation) ejaculated sperm as well as the aneuploidy rate in biopsied embryos from fertility cycles. Fluorescence in situ hybridization was used for the aneuploidy analysis. Results were compared using linear regression and analysis of variance.
  
  
  RESULTS
  A total of 154 embryos were evaluated from 38 patients undergoing PGD cycles; 35.2% of the embryos were chromosomally normal. Analysis of the same sperm samples showed an increased DNA fragmentation after sperm preparation in 76% of the patients. There was no correlation between DNA fragmentation and the aneuploidy rate in embryos or in fresh or processed sperm samples.
  
  
  CONCLUSIONS
  Sperm DNA fragmentation is not related to chromosomal anomalies in embryos from patients with recurrent miscarriage or implantation failure. However, we cannot rule out the possibility that a relationship between DNA fragmentation and aneuploidy exists for other causes of infertility. Furthermore, the different methods used to evaluate DNA fragmentation may produce different results.
authors.affiliation: Departamento de Diagnostico Genetico Preimplantacional, IVI Madrid, Madrid 28023, Spain. fernando.bronet@ivi.es
authors.forename: F
authors.initials: F
authors.lastname: Bronet
authors.forename: E
authors.initials: E
authors.lastname: Martínez
authors.forename: M
authors.initials: M
authors.lastname: Gaytán
authors.forename: A
authors.initials: A
authors.lastname: Liñán
authors.forename: D
authors.initials: D
authors.lastname: Cernuda
authors.forename: M
authors.initials: M
authors.lastname: Ariza
authors.forename: M
authors.initials: M
authors.lastname: Nogales
authors.forename: A
authors.initials: A
authors.lastname: Pacheco
authors.forename: M
authors.initials: M
authors.lastname: San Celestino
authors.forename: J A
authors.initials: JA
authors.lastname: Garcia-Velasco
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D000782:Aneuploidy; D002499:Centrifugation, Density Gradient; D004249:DNA Damage; D053938:DNA Fragmentation; D010064:Embryo Implantation; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D008423:Maternal Age; D009865:Oocytes / Q000166:cytology; D010062:Ovulation Induction; D010331:Paternal Age; D012107:Research Design; D013094:Spermatozoa / Q000473:pathology*