title: Protection of pigs against Taenia solium cysticercosis by immunization with novel recombinant antigens.
abstract: Recombinant antigens from the oncosphere stage of the parasite Taenia solium were expressed in Escherichia coli. The TSOL16, TSOL45-1A and TSOL45-1B recombinant antigens, each consisting of fibronectin type III (FnIII) domain S, were produced as fusion proteins with glutathione S-transferase (GST) and maltose binding protein (MBP). Groups of pigs were immunized twice with the GST fusions of the antigens and boosted a third time with the MBP fusions prior to receiving a challenge infection with T. solium eggs. The TSOL16 antigen was found to be capable of inducing high levels of immunity in pigs against a challenge infection with T. solium. Immunological investigations identified differences in immune responses in the pigs vaccinated with the various antigens. The results demonstrate that the TSOL16 antigen could be a valuable adjunct to current porcine vaccination approaches and may allow the further development of new vaccination strategies against T. solium cysticercosis.
authors.affiliation: University of Melbourne, Faculty of Veterinary Science, Werribee, Victoria 3030, Australia. charlesg@unimelb.edu.au
authors.forename: Charles G
authors.initials: CG
authors.lastname: Gauci
authors.forename: César M
authors.initials: CM
authors.lastname: Jayashi
authors.forename: Armando E
authors.initials: AE
authors.lastname: Gonzalez
authors.forename: Julia
authors.initials: J
authors.lastname: Lackenby
authors.forename: Marshall W
authors.initials: MW
authors.lastname: Lightowlers
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D003551:Cysticercosis / Q000517:prevention & control / Q000662:veterinary*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D007114:Immunization / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D041201:Taenia solium / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic