title: A one-step process in preparation of cationic nanoparticles with poly(lactide-co-glycolide)-containing polyethylenimine gives efficient gene delivery.
abstract: A one-step preparation of nanoparticles with poly(lactide-co-glycolide) (PLGA) pre-modified with polyethylenimine (PEI) is better in requirements for DNA delivery compared to those prepared in a two-step process (preformed PLGA nanoparticles and subsequently coated with PEI). The particles were prepared by emulsification of PLGA/ethyl acetate in an aqueous solution of PVA and PEI. DLS, AFM and SEM were used for the size characteristics. The cytotoxicity of PLGA/PEI nanoparticles was detected by MTT assay. The transfection activity of the particles was measured using pEGFP and pβ-gal plasmid DNA. Results showed that the PLGA/PEI nanoparticles were spherical and non-porous with a size of about 0.2 μm and a small size distribution. These particles had a positive zeta potential demonstrating that PEI was attached. Interestingly, the zeta potential of the particles (from one-step procedure) was substantially higher than that of two-step process and is ascribed to the conjugation of PEI to PLGA via aminolysis. The PLGA/PEI nanoparticles were able to bind DNA and the formed complexes had a substantially lower cytotoxicity and a higher transfection activity than PEI polyplexes. In conclusion, given their small size, stability, low cytotoxicity and good transfection activity, PLGA/PEI-DNA complexes are attractive gene delivery systems.
authors.affiliation: Department of Biotechnology, Chia-Nan University of Pharmacy and Science, 60 Erh-Jen Rd., Sec. 1, Jen-Te, Taiwan.
authors.forename: Min Da
authors.initials: MD
authors.lastname: Shau
authors.forename: Mei Fen
authors.initials: MF
authors.lastname: Shih
authors.forename: Chi Cheng
authors.initials: CC
authors.lastname: Lin
authors.forename: I Chuan
authors.initials: IC
authors.lastname: Chuang
authors.forename: Wei Chih
authors.initials: WC
authors.lastname: Hung
authors.forename: Wim E
authors.initials: WE
authors.lastname: Hennink
authors.forename: Jong Yuh
authors.initials: JY
authors.lastname: Cherng
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D019556:COS Cells; D002412:Cations; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry* / Q000633:toxicity; D011098:Polyglactin 910 / Q000737:chemistry* / Q000633:toxicity; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013499:Surface Properties; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000085:Acetates; D002412:Cations; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011098:Polyglactin 910; C007650:ethyl acetate; D011142:Polyvinyl Alcohol; D011094:Polyethyleneimine; D004247:DNA; D001616:beta-Galactosidase