title: Catalases negatively regulate methyl jasmonate signaling in guard cells.
abstract: Methyl jasmonate (MeJA)-induced stomatal closure is accompanied by the accumulation of hydrogen peroxide (H₂O₂) in guard cells. In this study, we investigated the roles of catalases (CATs) in MeJA-induced stomatal closure using cat mutants cat2, cat3-1 and cat1 cat3, and the CAT inhibitor, 3-aminotriazole (AT). When assessed with 2',7'-dichlorodihydrofluorescein, the reduction of catalase activity by means of mutations and the inhibitor accumulated higher basal levels of H₂O₂ in guard cells whereas they did not affect stomatal aperture in the absence of MeJA. In contrast, the cat mutations and the treatment with AT potentiated MeJA-induced stomatal closure and MeJA-induced H₂O₂ production. These results indicate that CATs negatively regulate H₂O₂ accumulation in guard cells and suggest that inducible H₂O₂ production rather than constitutive elevation modulates stomatal apertures in Arabidopsis.
authors.affiliation: Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan.
authors.forename: Rayhanur
authors.initials: R
authors.lastname: Jannat
authors.forename: Misugi
authors.initials: M
authors.lastname: Uraji
authors.forename: Mohammad Anowar
authors.initials: MA
authors.lastname: Hossain
authors.forename: Mohammad Muzahidul
authors.initials: MM
authors.lastname: Islam
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Izumi C
authors.initials: IC
authors.lastname: Mori
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Murata
mesh_terms: D000085:Acetates / Q000378:metabolism* / Q000494:pharmacology; D000640:Amitrole / Q000494:pharmacology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D002374:Catalase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology; D054046:Plant Stomata / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D004791:Enzyme Inhibitors; D054883:Oxylipins; D017382:Reactive Oxygen Species; C072239:methyl jasmonate; D002374:Catalase; D000640:Amitrole