title: Fluorometric detection of adenine in target DNA by exciplex formation with fluorescent 8-arylethynylated deoxyguanosine.
abstract: We demonstrated an intriguing method to discriminate adenine by incident appearance of an intense new emission via exciplex formation in hybridization of target DNA with newly designed fluorescent 8-arylethynylated deoxyguanosine derivatives. We described the synthesis of such highly electron donating fluorescent guanosine derivatives and their incorporation into DNA oligomers which may be used for the structural study and the fluorometric analysis of nucleic acids.
authors.affiliation: Department of Chemical Biology and Applied Chemistry, School of Engineering, Nihon University, Koriyama, Fukushima, Japan. saitoy@chem.ce.nihon-u.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Saito
authors.forename: Kenji
authors.initials: K
authors.lastname: Kugenuma
authors.forename: Makiko
authors.initials: M
authors.lastname: Tanaka
authors.forename: Azusa
authors.initials: A
authors.lastname: Suzuki
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
mesh_terms: D000225:Adenine / Q000032:analysis*; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D003849:Deoxyguanosine / Q000031:analogs & derivatives*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D004247:DNA; D003849:Deoxyguanosine; D000225:Adenine