title: Synthesis of chiral cyanohydrins by recombinant Escherichia coli cells in a micro-aqueous reaction system.
abstract: Synthesis of chiral cyanohydrins is performed in a monophasic micro-aqueous reaction system using whole recombinant Escherichia coli cells expressing the Arabidopsis thaliana hydroxynitrile lyase (AtHNL). Microscopy studies employing a fusion of AtHNL with a flavin-based fluorescent protein (FbFP) reveal that the cells remain intact in the reaction system.
authors.affiliation: Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf and Forschungszentrum Jülich, Jülich, Germany.
authors.forename: Kathrin Emmi
authors.initials: KE
authors.lastname: Scholz
authors.forename: Daniel
authors.initials: D
authors.lastname: Okrob
authors.forename: Benita
authors.initials: B
authors.lastname: Kopka
authors.forename: Alexander
authors.initials: A
authors.lastname: Grünberger
authors.forename: Martina
authors.initials: M
authors.lastname: Pohl
authors.forename: Karl-Erich
authors.initials: KE
authors.lastname: Jaeger
authors.forename: Ulrich
authors.initials: U
authors.lastname: Krauss
mesh_terms: D000446:Aldehyde-Lyases / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D009570:Nitriles / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D009570:Nitriles; D011993:Recombinant Fusion Proteins; C430593:cyanohydrin; D000446:Aldehyde-Lyases; C021541:mandelonitrile lyase