title: P65 truncation impacts P30 dynamics during Mycoplasma pneumoniae gliding.
abstract: The cell wall-less prokaryote Mycoplasma pneumoniae is a major cause of community-acquired bronchitis and pneumonia in humans. Colonization is mediated largely by a differentiated terminal organelle, which is also the leading end in gliding motility. Cytadherence-associated proteins P30 and P65 appear to traffic concurrently to the distal end of developing terminal organelles. Here, truncation of P65 due to transposon insertion in the corresponding gene resulted in lower gliding velocity, reduced cytadherence, and decreased steady-state levels of several terminal organelle proteins, including P30. Utilizing fluorescent protein fusions, we followed terminal organelle development over time. New P30 foci appeared at nascent terminal organelles in P65 mutants, as in the wild type. However, with forward cell motility, P30 in the P65 mutants appeared to drag toward the trailing cell pole, where it was released, yielding a fluorescent trail to which truncated P65 colocalized. In contrast, P30 was only rarely observed at the trailing end of gliding wild-type cells. Complementation with the recombinant wild-type P65 allele by transposon delivery restored P65 levels and stabilized P30 localization to the terminal organelle.
authors.affiliation: Department of Microbiology, University of Georgia, Athens, GA, USA.
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Hasselbring
authors.forename: Edward S
authors.initials: ES
authors.lastname: Sheppard
authors.forename: Duncan C
authors.initials: DC
authors.lastname: Krause
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D009177:Mycoplasma pneumoniae / Q000737:chemistry / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism; D021381:Protein Transport; D017384:Sequence Deletion
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; C093832:Mycoplasma protein 65-kDa; C096205:P30 adhesin, Mycoplasma