title: Neuroprotective compounds isolated from Cynanchum paniculatum.
abstract: Ten compounds were isolated from the 80% methanol extract of roots of Cynanchum paniculatum Kitagawa (Asclepiadaceae) and identified as paeonol (1), 4-acetylphenol (2), 2,5-dihydroxy-4-methoxyacetophenone (3), 2,3-dihydroxy-4-methoxyacetophenone (4), acetoveratrone (5), 2,5-dimethoxyhydroquinone (6), vanillic acid (7), resacetophenone (8), m-acetylphenol (9) and 3,5-dimethoxyhydroquinone (10). The protective effect of compounds 1-10 against glutamate-induced cytotoxicity in hippocampal HT22 cell line was evaluated. Among them, compound 4 showed a relatively effective protection of 47.55% (at 10 μM). This result suggested that compounds 4 had neuroprotective effect on the glutamate-induced neurotoxicity in HT22 cells.
authors.affiliation: Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 200-701, Korea.
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.forename: Chul Young
authors.initials: CY
authors.lastname: Kim
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Yang
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D031105:Cynanchum / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000494:pharmacology; D051379:Mice; D015394:Molecular Structure; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020258:Neurotoxicity Syndromes / Q000517:prevention & control; D018517:Plant Roots / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D018698:Glutamic Acid