title: Prebiotics and bioactive natural substances induce changes of composition and metabolic activities of the colonic microflora in cancerous rats.
abstract: Prebiotics are defined as selectively fermented food ingredients that induce specific changes in the composition and/or activity in the gastrointestinal microbiota beneficial to the host well-being and health. The aim of the presented experiment was to investigate the effect of a prebiotic applied alone or in combination with Hyppocastani extractum siccum, and Lini oleum virginale in rats with dimethylhydrazine induced colon cancer. Wistar albino rats were fed high fat diet supplemented with the prebiotic alone or in combination with Horse chestnut and flaxseed oil. The activity of faecal glycolytic enzymes, lipid parameters, bile acids, short chain fatty acids and counts of coliforms and lactobacilli were determined. Treatment with the prebiotic alone and in combination with selected substances significantly decreased the activity of glycolytic bacterial enzyme β-glucuronidase (P<0.001) and increased activities of β-galactosidase and β-glucosidase. Bile acids concentration was significantly decreased (P<0.01) except for the combination of the prebiotic with Horse chestnut. The prebiotic alone decreased the lipid parameters (P<0.001) and enhanced production of short chain fatty acids. Application of prebiotic and bioactive natural substances significantly reduced number of coliforms (P<0.05). Prebiotic alone significantly increased the count of lactobacilli (P<0.05). These results show that prebiotics have a protective effect and may be the useful for colon cancer prevention and treatment.
authors.affiliation: Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic. hijova@pobox.sk
authors.forename: Emília
authors.initials: E
authors.lastname: Hijová
authors.forename: Alojz
authors.initials: A
authors.lastname: Bomba
authors.forename: Izabela
authors.initials: I
authors.lastname: Bertková
authors.forename: Ladislav
authors.initials: L
authors.lastname: Strojný
authors.forename: Viktória
authors.initials: V
authors.lastname: Szabadosová
authors.forename: Alena
authors.initials: A
authors.lastname: Šoltésová
mesh_terms: D019813:1,2-Dimethylhydrazine; D031319:Aesculus / Q000737:chemistry; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001647:Bile Acids and Salts / Q000097:blood; D003106:Colon / Q000382:microbiology*; D003110:Colonic Neoplasms / Q000097:blood / Q000139:chemically induced / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005243:Feces / Q000737:chemistry / Q000201:enzymology / Q000382:microbiology; D005696:Galactosidases / Q000737:chemistry / Q000378:metabolism; D005959:Glucosidases / Q000737:chemistry / Q000378:metabolism; D005966:Glucuronidase / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007444:Inulin / Q000008:administration & dosage*; D007778:Lactobacillus / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008043:Linseed Oil / Q000008:administration & dosage*; D008055:Lipids / Q000097:blood; D010936:Plant Extracts / Q000008:administration & dosage*; D056692:Prebiotics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001426:Bacterial Proteins; D001647:Bile Acids and Salts; D005227:Fatty Acids; D008055:Lipids; D010936:Plant Extracts; D056692:Prebiotics; D008043:Linseed Oil; D007444:Inulin; D005696:Galactosidases; D005959:Glucosidases; D005966:Glucuronidase; D019813:1,2-Dimethylhydrazine