title: Trypanocidal activity of human plasma on Trypanosoma evansi in mice.
abstract: This study aimed to test an alternative protocol with human plasma to control Trypanosoma evansi infection in mice. Plasma from an apparently 27-year-old healthy male, blood type A+, was used in the study. A concentration of 100 mg.dL(-1) apolipoprotein L1 (APOL1) was detected in the plasma. Forty mice were divided into four groups with 10 animals each. Group A comprised uninfected animals. Mice from groups B, C and D were inoculated with a T. evansi isolate. Group B was used as a positive control. At three days post-infection (DPI), the mice were administered intraperitoneally with human plasma. A single dose of 0.2 mL plasma was given to those in group C. The mice from group D were administered five doses of 0.2 mL plasma with a 24 hours interval between the doses. Group B showed high increasing parasitemia that led to their death within 5 DPI. Both treatments eliminated parasites from the blood and increased the longevity of animals. An efficacy of 50 (group C) and 80% (group D) of human plasma trypanocidal activity was found using PCR. This therapeutic success was likely achieved in the group D due to their higher levels of APOL1 compared with group C.
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria-UFSM, Prédio 20, Sala 4232, Campus Universitário, Camobi, CEP 97105-900, Santa Maria, RS, Brasil. aleksandro_ss@yahoo.com.br
authors.forename: Aleksandro Schafer
authors.initials: AS
authors.lastname: Da Silva
authors.forename: Marcos Rafael Kroeker
authors.initials: MR
authors.lastname: Duck
authors.forename: Vinicius da Rosa
authors.initials: Vda R
authors.lastname: Fanfa
authors.forename: Mateus Anderson
authors.initials: MA
authors.lastname: Otto
authors.forename: João Tomaz Schmitt
authors.initials: JT
authors.lastname: Nunes
authors.forename: Alexandre Alberto
authors.initials: AA
authors.lastname: Tonin
authors.forename: Jeandre Augusto
authors.initials: JA
authors.lastname: Jaques
authors.forename: Francine Chimelo
authors.initials: FC
authors.lastname: Paim
authors.forename: Marta Maria Medeiros Frescura
authors.initials: MM
authors.lastname: Duarte
authors.forename: Silvia Gonzalez
authors.initials: SG
authors.lastname: Monteiro
mesh_terms: D000328:Adult; D000818:Animals; D001790:Blood Physiological Phenomena; D006801:Humans; D008297:Male; D051379:Mice; D010949:Plasma; D014345:Trypanosoma