title: Genetic variation of Haemonchus contortus (Trichostrongylidae) in sheep and goats from Malaysia and Yemen.
abstract: The large stomach worm, Haemonchus contortus, commonly known as "the barber's pole worm", is a blood-sucking nematode found in the abomasa of sheep and goats. This work is the first documentation on the ND4 sequences of H. contortus from sheep and goats in Malaysia and Yemen and the results provide a preliminary insight on the genetic differences of H. contortus found in the two countries. In general, this study showed a high degree of diversity and low population structure of this species within the same country in comparison with higher genetic structuring at a wider geographical scale. The results also showed that the majority of genetic variance was within H. contortus populations. The Malaysian sheep and goat populations investigated appeared to share the same isolate of H. contortus while different isolates may be found in Yemen which must be taken into account in the design of an effective control strategy. Analysis of the internal transcribed spacer-2 (ITS-2) confirmed that all samples investigated in this study belonged to H. contortus. However presence of other Haemonchus species parasitizing these two hosts can only be confirmed by further detailed studies.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. ghrama77@yahoo.com
authors.forename: A A
authors.initials: AA
authors.lastname: Gharamah
authors.forename: M N Siti
authors.initials: MN
authors.lastname: Azizah
authors.forename: W A
authors.initials: WA
authors.lastname: Rahman
mesh_terms: D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D006041:Goats; D006188:Haemonchiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000145:classification / Q000235:genetics*; D008296:Malaysia / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011897:Random Allocation; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000469:parasitology*; D015006:Yemen / Q000453:epidemiology
chemical_list: D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer