title: Development of an immunoassay for the derived-peptide of chromogranin A, vasostatin-I (1-76): assessment of severity in patients with sepsis.
abstract: BACKGROUND
  Proteolytic fragments of chromogranin A (CgA) including the CgA 1-76 fragment (called vasostatin-I [VS-I]) could be a useful biomarker of sepsis, but there is no available immunoassay.
  
  
  METHODS
  A sandwich ELISA for VS-I was developed, and plasma VS-I was measured in 30 healthy controls and 60 critically ill patients with sepsis.
  
  
  RESULTS
  The ELISA showed intra- and inter-assay coefficients of variations (CVs) below 4 and 9%. Plasma VS-I was significantly increased compared with controls in patients with sepsis, severe sepsis, and sepsis shock (p < 0.0001). Receiver operating curve (ROC) analyses indicated that plasma VS-I was more sensitive and specific than plasma CgA to diagnose sepsis and to assess its severity.
  
  
  CONCLUSIONS
  The measurements of plasma VS-I with this new ELISA may be useful for the clinical investigation of patients with sepsis.
authors.affiliation: Cisbio Bioassays, Codolet, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Chung
authors.forename: Angelo
authors.initials: A
authors.lastname: Corti
authors.forename: Luca
authors.initials: L
authors.lastname: Crippa
authors.forename: Francis
authors.initials: F
authors.lastname: Schneider
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Metz-Boutigue
authors.forename: Patrick
authors.initials: P
authors.lastname: Garnero
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D053379:Chromogranin A / Q000097:blood*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood*; D012372:ROC Curve; D015203:Reproducibility of Results; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis; D012720:Severity of Illness Index; D012772:Shock, Septic / Q000097:blood / Q000175:diagnosis
chemical_list: D015415:Biomarkers; D053379:Chromogranin A; D010446:Peptide Fragments; C064110:vasostatin I