title: High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans.
abstract: We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.
authors.affiliation: Department of Genetics, Bio21 Institute, The University of Melbourne, Victoria, Australia. ronaldl@unimelb.edu.au
authors.forename: Siu F
authors.initials: SF
authors.lastname: Lee
authors.forename: Vanessa L
authors.initials: VL
authors.lastname: White
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Weeks
authors.forename: Ary A
authors.initials: AA
authors.lastname: Hoffmann
authors.forename: Nancy M
authors.initials: NM
authors.lastname: Endersby
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D004330:Drosophila / Q000382:microbiology*; D005453:Fluorescence; D057166:High-Throughput Screening Assays / Q000191:economics / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D020577:Wolbachia / Q000235:genetics* / Q000302:isolation & purification*