title: Long-term results of cell-free biodegradable scaffolds for in situ tissue-engineering vasculature: in a canine inferior vena cava model.
abstract: We have developed a new biodegradable scaffold that does not require any cell seeding to create an in-situ tissue-engineering vasculature (iTEV). Animal experiments were conducted to test its characteristics and long-term efficacy. An 8-mm tubular biodegradable scaffold, consisting of polyglycolide knitted fibers and an L-lactide and ε-caprolactone copolymer sponge with outer glycolide and ε-caprolactone copolymer monofilament reinforcement, was implanted into the inferior vena cava (IVC) of 13 canines. All the animals remained alive without any major complications until euthanasia. The utility of the iTEV was evaluated from 1 to 24 months postoperatively. The elastic modulus of the iTEV determined by an intravascular ultrasound imaging system was about 90% of the native IVC after 1 month. Angiography of the iTEV after 2 years showed a well-formed vasculature without marked stenosis or thrombosis with a mean pressure gradient of 0.51 ± 0.19 mmHg. The length of the iTEV at 2 years had increased by 0.48 ± 0.15 cm compared with the length of the original scaffold (2-3 cm). Histological examinations revealed a well-formed vessel-like vasculature without calcification. Biochemical analyses showed no significant differences in the hydroxyproline, elastin, and calcium contents compared with the native IVC. We concluded that the findings shown above provide direct evidence that the new scaffold can be useful for cell-free tissue-engineering of vasculature. The long-term results revealed that the iTEV was of good quality and had adapted its shape to the needs of the living body. Therefore, this scaffold would be applicable for pediatric cardiovascular surgery involving biocompatible materials.
authors.affiliation: Cardiovascular Surgery, The Heart Institute of Japan, Tokyo Women's Medical University, Shinjuku, Tokyo, Japan. smatumur@hij.twmu.ac.jp
authors.forename: Goki
authors.initials: G
authors.lastname: Matsumura
authors.forename: Naotaka
authors.initials: N
authors.lastname: Nitta
authors.forename: Shojiro
authors.initials: S
authors.lastname: Matsuda
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Noriko
authors.initials: N
authors.lastname: Isayama
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Ikada
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D002208:Caproates; D004285:Dogs; D007783:Lactones; D011100:Polyglycolic Acid; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D058017:Vascular Grafting / Q000379:methods*; D014682:Vena Cava, Inferior / Q000601:surgery*
chemical_list: D001672:Biocompatible Materials; D002208:Caproates; D007783:Lactones; D011100:Polyglycolic Acid; C121056:caprolactone