title: miRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR.
abstract: miRNAs are small molecules involved in gene regulation. Each tissue shows a characteristic miRNAs epression profile that could be altered during neoplastic transformation. Glioblastoma is the most aggressive brain tumour of the adult with a high rate of mortality. Recognizing a specific pattern of miRNAs for GBM could provide further boost for target therapy. The availability of fresh tissue for brain specimens is often limited and for this reason the possibility of starting from formalin fixed and paraffin embedded tissue (FFPE) could very helpful even in miRNAs expression analysis. We analysed a panel of 19 miRNAs in 30 paired samples starting both from FFPE and Fresh/Frozen material. Our data revealed that there is a good correlation in results obtained from FFPE in comparison with those obtained analysing miRNAs extracted from Fresh/Frozen specimen. In the few cases with a not good correlation value we noticed that the discrepancy could be due to dissection performed in FFPE samples. To the best of our knowledge this is the first paper demonstrating that the results obtained in miRNAs analysis using Real-Time PCR starting from FFPE specimens of glioblastoma are comparable with those obtained in Fresh/Frozen samples.
authors.affiliation: Department of Patologia Sperimentale, University of Bologna, Bologna, Italy. dario.debiase@unibo.it
authors.forename: Dario
authors.initials: D
authors.lastname: de Biase
authors.forename: Michela
authors.initials: M
authors.lastname: Visani
authors.forename: Luca
authors.initials: L
authors.lastname: Morandi
authors.forename: Gianluca
authors.initials: G
authors.lastname: Marucci
authors.forename: Cristian
authors.initials: C
authors.lastname: Taccioli
authors.forename: Serenella
authors.initials: S
authors.lastname: Cerasoli
authors.forename: Agostino
authors.initials: A
authors.lastname: Baruzzi
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pession
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics*; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D016612:Paraffin Embedding; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D005404:Fixatives; D035683:MicroRNAs; D005557:Formaldehyde