title: Inhibitory effects of Carpinus tschonoskii leaves extract on CpG-stimulated pro-inflammatory cytokine production in murine bone marrow-derived macrophages and dendritic cells.
abstract: This report describes the anti-inflammatory effects of MeOH extract from leaves of Carpinus tschonoskii (CE) on primary bone marrow-derived macrophage (BMDMs) and dendritic cells (BMDCs). Primary BMDMs and BMDCs were used for pro-inflammatory cytokine production and Western blot analysis. Human embryonic kidney cell line 293 T (HEK293 T) was used to access NF-κB activity. In all cases, CpG DNA was used to stimulate the cells. The CE (0-150 μg/ml) was treated to BMDMs, BMDCs, and HEK293T cells. CE pre-treatment in CpG-stimulated BMDMs and BMDCs showed a dose-dependent inhibitory effect on pro-inflammatory cytokine (e.g., IL-12 p40, IL-6, and TNF-α) production as compared to non-treated controls. The CE pre-treatment had no significant inhibition on mitogen-activated protein kinases (MAPKs) phosphorylation but strongly inhibited IκBα degradation. In NF-κB reporter gene assay, the CE pre-treatment inhibited NF-κB-dependent luciferase activity in a dose-dependent manner. Taken together, these data suggest that CE has significant inhibitory effect on pro-inflammatory cytokine production and warrant further studies concerning potentials of CE for medicinal uses.
authors.affiliation: School of Medicine, Jeju National University, Jeju, South Korea.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Koo
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Hong
authors.forename: Vivek Bhakta
authors.initials: VB
authors.lastname: Mathema
authors.forename: Hee-Kyoung
authors.initials: HK
authors.lastname: Kang
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Hyun
authors.forename: Gi-Young
authors.initials: GY
authors.lastname: Kim
authors.forename: Young-Ree
authors.initials: YR
authors.lastname: Kim
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Maeng
authors.forename: Chang-Lim
authors.initials: CL
authors.lastname: Hyun
authors.forename: Weon-Young
authors.initials: WY
authors.lastname: Chang
authors.forename: Young-Sang
authors.initials: YS
authors.lastname: Koh
mesh_terms: D000818:Animals; D029401:Betulaceae / Q000737:chemistry*; D016207:Cytokines / Q000378:metabolism*; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: C408982:CPG-oligonucleotide; D016207:Cytokines; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D010936:Plant Extracts