title: Improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles.
abstract: The activity and half-life of pectate lyase (PL) from Bacillus megaterium were nine- and 60-fold, respectively, higher at 90 °C in the presence of hydroxyapatite nanoparticles (NP-PLs) than in the presence of 1mM CaCl(2). Thermodynamic analysis of the nanoparticle-induced stability revealed an enhanced entropy-enthalpy compensation by the NP-PLs since a reciprocal linearity of the enthalpy-entropy change to 90 °C was observed. Without nanoparticles, the linearity range was 70 °C. Such compensation reflected the maintenance of the native structure of proteins. The remarkable enhancement of activity and stability of the NP-PL system at high temperatures may be utilized commercially e.g. in the food industry or the processing of natural fibers that may require a thermotolerant enzyme.
authors.affiliation: Department of Biochemistry, University College of Science, Calcutta University, 35 Ballygunge Circular Road, West Bengal, Kolkata 700 019, India.
authors.forename: Arka
authors.initials: A
authors.lastname: Mukhopadhyay
authors.forename: Anjan Kumar
authors.initials: AK
authors.lastname: Dasgupta
authors.forename: Dhrubajyoti
authors.initials: D
authors.lastname: Chattopadhyay
authors.forename: Krishanu
authors.initials: K
authors.lastname: Chakrabarti
mesh_terms: D001410:Bacillus megaterium / Q000201:enzymology; D002118:Calcium / Q000378:metabolism; D017886:Durapatite / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D019277:Entropy; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D006207:Half-Life; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry*; D011133:Polysaccharide-Lyases / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature
chemical_list: D017886:Durapatite; D011133:Polysaccharide-Lyases; C023911:pectate lyase; D002118:Calcium