title: Comparative characterisation of genotypically different clones of MRSA in the production of biofilms.
abstract: The ability to adhere and produce biofilms is characteristic of enhanced virulence among isolates of methicillin-resistant Staphylococcus aureus (MRSA). The aim of the study is to find out whether these characteristics are consistently similar among isolates variations of MRSA. The study used 30 various isolates of MRSA belong to 13 spa types and 5 MLST types and determined the aggregation, the adherence, and the production of biofilms and slime for each isolate. The methods used to evaluate these characteristics were a modified Congo red agar assay (MCRA), a microtiter plate assay (MPA), high-magnification light microscopy, scanning electron microscopy (SEM), and PCR. The study found that isolates belonging to similar Spa, SCCmec, and ST types have similar abilities to produce biofilms; however, their ability to produce slime on CRA was found to be different. Moreover, isolates that have different Spa types showed high variation in their ability to produce biofilms. The results of light microscope revealed the isolates that produced strong and weak biofilms and formed similar aggregation on the glass surfaces. SEM results showed that all 30 MRSA isolates that were tested were 100% positive for biofilm formation, although to varying degrees. Further testing using PCR confirmed that 100% of the 30 isolates tested were positive for the presence of the icaADBC, fnbA, eno, ebps, clfA, and clfB genes. The prevalence of fib, cna, fnbB, and bbp in MRSA clones was 90, 93.33, 53.33, and 10%, respectively. This study indicate that differences in biofilm production capacities are caused by the differences in surface protein A (Spa) type and are not due to differences in MLST and SCCmec types.
authors.affiliation: Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, Selangor, Serdang, Malaysia. salmanatshan@yahoo.com
authors.forename: Salman Sahab
authors.initials: SS
authors.lastname: Atshan
authors.forename: Mariana Nor
authors.initials: MN
authors.lastname: Shamsudin
authors.forename: Leslie Than Thian
authors.initials: LT
authors.lastname: Lung
authors.forename: Zamberi
authors.initials: Z
authors.lastname: Sekawi
authors.forename: Ehsanollah
authors.initials: E
authors.lastname: Ghaznavi-Rad
authors.forename: Chong Pei
authors.initials: CP
authors.lastname: Pei
mesh_terms: D000704:Analysis of Variance; D001422:Bacterial Adhesion / Q000235:genetics; D015373:Bacterial Typing Techniques; D018441:Biofilms / Q000145:classification*; D003224:Congo Red; D005798:Genes, Bacterial / Q000235:genetics; D005838:Genotype; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000502:physiology*; D008853:Microscopy; D058885:Multilocus Sequence Typing; D013203:Staphylococcal Infections / Q000382:microbiology; D013205:Staphylococcal Protein A / Q000235:genetics
chemical_list: D013205:Staphylococcal Protein A; D003224:Congo Red