title: An orthogonal comparison of the proteome of human embryonic stem cells with that of human induced pluripotent stem cells of different genetic background.
abstract: Induced pluripotent stem cells (iPSCs) provide an invaluable resource for drug or toxicology screening, medical research and patient-specific cell therapy. However, the potential applications of iPSCs are largely dependent on the degree of similarity between iPSCs and embryonic stem cells (ESCs). In the present study, we analyzed the proteome of human ESCs and hiPSCs with different genetic background. We carried out an orthogonal contrast analysis of the proteome pattern of two human ESC lines (Royan H5 and Royan H6) and two hiPSC lines from a normal individual, three hiPSC lines from a normal individual with Bombay blood group phenotype, and two hiPSC lines from a patient with tyrosinemia. Forty-nine protein spots showed statistically significant differences between two human ESC lines and seven human iPSCs. Mass spectrometry analysis resulted in the identification of 48 proteins belonging to different biological processes, including cytoskeleton organization, energy and metabolic processes, protein synthesis and processing, signal transduction, cell growth and proliferation, cellular trafficking, transcription, calcium binding and immune response. Our results showed that hESCs and hiPSCs had subtle differences at the proteome level thus warranting more detailed and systematic examinations of these cells.
authors.affiliation: Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Mojtaba Zamani
authors.initials: MZ
authors.lastname: Faradonbeh
authors.forename: Javad
authors.initials: J
authors.lastname: Gharechahi
authors.forename: Sepideh
authors.initials: S
authors.lastname: Mollamohammadi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Pakzad
authors.forename: Adeleh
authors.initials: A
authors.lastname: Taei
authors.forename: Hassan
authors.initials: H
authors.lastname: Rassouli
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
authors.forename: Ghasem Hosseini
authors.initials: GH
authors.lastname: Salekdeh
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics; D002460:Cell Line; D002478:Cells, Cultured; D016000:Cluster Analysis; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D053595:Embryonic Stem Cells / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D010641:Phenotype; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D040901:Proteomics; D020176:Tyrosinemias / Q000235:genetics
chemical_list: D000017:ABO Blood-Group System; D020543:Proteome