title: Will a peripheral blood (PB) sample yield the same diagnostic and prognostic cytogenetic data as the concomitant bone marrow (BM) in myelodysplasia?
abstract: In patients with myelodysplastic syndromes (MDS), chromosome anomalies are detected by conventional cytogenetic studies (CCS) and/or interphase fluorescence in situ hybridization (FISH) of bone marrow (BM) samples and provide prognostic and diagnostic information, which can direct therapy. Whether peripheral blood (PB) can be substituted for bone marrow in these cases and can provide the same information remains unknown. Concurrent BM and PB specimens collected from 100 patients with recently diagnosed MDS were studied using both CCS and FISH. While 68% of BM samples showed an abnormal karyotype by CCS, only 31% of PB samples were abnormal by CCS. In 12% of patients, FISH and CCS were discordant due to the inability of the FISH panel to detect all possible abnormalities. However, only one case (1%) had a cryptic abnormality detected by FISH. BM and PB FISH were discordant in 3% of cases, most likely due to the smaller clone size in PB vs. BM. While PB should not be substituted for BM at diagnosis, it is a viable alternative for monitoring patients using the appropriate FISH probe(s).
authors.affiliation: Stanford Hospitals and Clinics, Palo Alto, CA 94304, USA. acherry@stanfordmed.org
authors.forename: Athena M
authors.initials: AM
authors.lastname: Cherry
authors.forename: Marilyn L
authors.initials: ML
authors.lastname: Slovak
authors.forename: Lynda J
authors.initials: LJ
authors.lastname: Campbell
authors.forename: Kathy
authors.initials: K
authors.lastname: Chun
authors.forename: Virginie
authors.initials: V
authors.lastname: Eclache
authors.forename: Detlef
authors.initials: D
authors.lastname: Haase
authors.forename: Claudia
authors.initials: C
authors.lastname: Haferlach
authors.forename: Barbara
authors.initials: B
authors.lastname: Hildebrandt
authors.forename: Anwar M
authors.initials: AM
authors.lastname: Iqbal
authors.forename: Suresh C
authors.initials: SC
authors.lastname: Jhanwar
authors.forename: Kazuma
authors.initials: K
authors.lastname: Ohyashiki
authors.forename: Francesc
authors.initials: F
authors.lastname: Sole
authors.forename: Peter
authors.initials: P
authors.lastname: Vandenberghe
authors.forename: Daniel L
authors.initials: DL
authors.lastname: VanDyke
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
authors.forename: Gordon W
authors.initials: GW
authors.lastname: Dewald
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle; D001856:Bone Marrow Examination / Q000379:methods; D002648:Child; D020732:Cytogenetic Analysis / Q000379:methods*; D003582:Cytogenetics / Q000379:methods; D005260:Female; D006403:Hematologic Tests / Q000379:methods; D006801:Humans; D007621:Karyotyping / Q000379:methods; D008297:Male; D008495:Medical Oncology / Q000379:methods*; D008875:Middle Aged; D008954:Models, Biological; D009190:Myelodysplastic Syndromes / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D011379:Prognosis; D055815:Young Adult