title: Capacitance-based assay for real-time monitoring of endocytosis and cell viability.
abstract: Label-free cell-based assays have emerged as a promising means for high-throughput screening. Most label-free sensors are based on impedance measurements that reflect the passive electrical properties of cells. Here we introduce a capacitance-based assay that measures the dielectric constant (capacitance) of biological cells, and demonstrate the feasibility of analyzing endocytosis and screening chemotherapeutic agents with this assay. Endocytosis induces a change in the zeta potential, leading to a change in the dielectric constant which enables real-time endocytosis monitoring using the capacitance sensor. Additionally, since the dielectric constant is proportional to cell radius and cell volume, cell viability can be estimated from the change in capacitance. Therefore, the capacitance sensor array can also be used for cytotoxicity testing for large-scale chemotherapeutic screening.
authors.affiliation: Graduate Program for Nanomedical Science and Technology, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Rimi
authors.initials: R
authors.lastname: Lee
authors.forename: Jihun
authors.initials: J
authors.lastname: Kim
authors.forename: Sook Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Seon Mi
authors.initials: SM
authors.lastname: Jang
authors.forename: Sun-Mi
authors.initials: SM
authors.lastname: Lee
authors.forename: In-Hong
authors.initials: IH
authors.lastname: Choi
authors.forename: Seung Woo
authors.initials: SW
authors.lastname: Park
authors.forename: Jeon-Soo
authors.initials: JS
authors.lastname: Shin
authors.forename: Kyung-Hwa
authors.initials: KH
authors.lastname: Yoo
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000970:Antineoplastic Agents / Q000633:toxicity; D045744:Cell Line, Tumor; D048429:Cell Size; D002470:Cell Survival / Q000187:drug effects*; D025802:Electric Capacitance; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004705:Endocytosis / Q000187:drug effects*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D010587:Phagocytosis; D010873:Pinocytosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology / Q000378:metabolism; D012834:Silver / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D018719:Erb-b2 Receptor Tyrosine Kinases; D012834:Silver; C508053:ERBB2 protein, human