title: Endozoicomonas numazuensis sp. nov., a gammaproteobacterium isolated from marine sponges, and emended description of the genus Endozoicomonas Kurahashi and Yokota 2007.
abstract: Two non-motile, rod-shaped gammaproteobacteria were isolated from marine sponges collected from the coast of Japan at Numazu. The isolates were oxidase- and catalase-positive facultative anaerobes that fermented carbohydrates. They required sodium ions for growth and were slightly halophilic, growing in the presence of 1.0-5.0 % (w/v) NaCl (optimum of 2.0 % NaCl). Under aerobic conditions, the major isoprenoid quinones were ubiquinone-9 and menaquinone-9 and the minor quinones were ubiquinone-8 and menaquinone-8. The major cellular fatty acids were C(18 : 1)ω7c, C(16 : 1)ω7c and C(16 : 0) and the hydroxy acids were C(10 : 0) 3-OH and C(12 : 0) 3-OH. The DNA G+C content was 48.3-48.7 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the isolates within the radiation of the genus Endozoicomonas in a broad clade of uncultured clones recovered from various marine invertebrates. The isolates exhibited 96.5-96.9 % 16S rRNA gene sequence similarity with Endozoicomonas elysicola MKT110(T) and Endozoicomonas montiporae CL-33(T), with which the isolates formed a monophyletic cluster with 100 % bootstrap support. The phenotypic features (carbohydrate fermentation, quinone system and some major cellular fatty acids) differed from those of members of the genus Endozoicomonas, which are aerobic, produce little or no menaquinone under aerobic conditions and possess different amounts of C(14 : 0) and C(18 : 1)ω7c. Although some phenotypic differences were identified, the isolates should be assigned to the genus Endozoicomonas on the basis of congruity of phylogeny and should be classified as representatives of a novel species, for which the name Endozoicomonas numazuensis sp. nov. is proposed. The type strain is HC50(T) ( = NBRC 108893(T)  = DSM 25634(T)). An emended description of the genus Endozoicomonas is presented.
authors.affiliation: TechnoSuruga Laboratory Co. Ltd, Nagasaki, Shimizu-ku, Shizuoka 424-0065, Japan.|Marine Biotechnology Institute, Heita, Kamaishi 026-0001, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Nishijima
authors.affiliation: Marine Biotechnology Institute, Heita, Kamaishi 026-0001, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Adachi
authors.affiliation: Marine Biotechnology Institute, Heita, Kamaishi 026-0001, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Katsuta
authors.affiliation: Marine Biotechnology Institute, Heita, Kamaishi 026-0001, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Shizuri
authors.affiliation: Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.|Marine Biotechnology Institute, Heita, Kamaishi 026-0001, Japan.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Yamasato
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology*; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S