title: Intracerebellar application of P19-derived neuroprogenitor and naive stem cells to Lurcher mutant and wild type B6CBA mice.
abstract: BACKGROUND
  Neurotransplantation has great potential for future treatments of various neurodegenerative disorders. Preclinically, the Lurcher mutant mouse represents an appropriate model of genetically-determined olivocerebellar degeneration. The aim of the present study was to assess survival of naïve and neurally differentiated P19 carcinoma stem cells following transplantation into the cerebellum of Lurcher mice and wild type littermates.
  
  
  METHODS
  Adult normal wild type (n=51) and Lurcher mutant mice (n=87) of the B6CBA strain were used. The mean age of the animals at the time of transplantation was 261.5 days. Suspension of naive and neurally differentiated P19 carcinoma stem cells was injected into the cerebellum of the mice. In the Lurcher mutants, 2 depths of graft injection were used. Three weeks after implantation the brains of experimental animals were examined histologically.
  
  
  RESULTS
  Survival of neuroprogenitor grafts at a depth of 1.6 mm was significantly higher in wild type vs. Lurcher mutant mice. In wild type mice, the typical graft localization was in the middle of the cerebellum, whereas in Lurcher mice the graft was never found inside the degenerated cerebellum and was primarily localized in the mesencephalon.
  
  
  CONCLUSIONS
  We conclude that the appearance and low survival rate of cerebellar P19 carcinoma stem cell grafts in the Lurcher mutant mice weigh against the therapeutic value of this cell line in preclinical studies of neurodegeneration.
authors.affiliation: Department of Pathophysiology, Faculty of Medicine in Pilsen, Charles University in Prague, Plzen, Czech Republic.
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Houdek
authors.forename: Jan
authors.initials: J
authors.lastname: Cendelín
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Kulda
authors.forename: Václav
authors.initials: V
authors.lastname: Babuška
authors.forename: Miroslava
authors.initials: M
authors.lastname: Cedíková
authors.forename: Milena
authors.initials: M
authors.lastname: Králíčková
authors.forename: Jiří
authors.initials: J
authors.lastname: Pacherník
authors.forename: George B
authors.initials: GB
authors.lastname: Stefano
authors.forename: František
authors.initials: F
authors.lastname: Vožeh
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000166:cytology*; D006085:Graft Survival; D051379:Mice; D008817:Mice, Mutant Strains; D014411:Neoplastic Stem Cells / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology*; D033581:Stem Cell Transplantation