title: Effect of the surface charge of artificial model membranes on the aggregation of amyloid β-peptide.
abstract: The neurotoxicity effect of the β-amyloid (Aβ) peptide, the primary constituent of senile plaques in Alzheimer's disease, occurs through interactions with neuronal membranes. Here, we attempt to clarify the mechanisms and consequences of the interaction of Aβ with lipid membranes. We have used liposomes as a model of biological membrane, and have devoted particular attention to the bilayer charge effect. Our results show that insertion and surface association of peptide with membrane, increased in a membrane charge-dependent manner, lead to a reduction of Aβ soluble species, lag time elongation and an increase in the inter-molecular β-sheet ratio of amyloid fibrils. In addition, our findings suggest that the fine balance between peptide insertion and surface association modulates Aβ aggregation, influencing the amyloid fibrils concentration as well as their morphology.
authors.affiliation: Departament de Bioquímica i Biologia Molecular and Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193-Bellaterra, Barcelona, Spain. raimon.sabate@uab.cat
authors.forename: Raimon
authors.initials: R
authors.lastname: Sabaté
authors.forename: Alba
authors.initials: A
authors.lastname: Espargaró
authors.forename: Lucyanna
authors.initials: L
authors.lastname: Barbosa-Barros
authors.forename: Salvador
authors.initials: S
authors.lastname: Ventura
authors.forename: Joan
authors.initials: J
authors.lastname: Estelrich
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D000682:Amyloid / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002463:Cell Membrane Permeability; D006801:Humans; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D008567:Membranes, Artificial; D058225:Plaque, Amyloid / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D008081:Liposomes; D008563:Membrane Lipids; D008567:Membranes, Artificial