title: Disinfection effect of dental impression tray adhesives.
abstract: OBJECTIVE
  Iatrogenic infections are serious problems in dental offices. Impression tray adhesives are delivered in glass containers with a fixed brush attached inside the cap. Using the brush for application of the impression tray adhesive on a contaminated impression tray or prostheses, pathogen transmission by replacing the cap with the brush is possible.
  
  
  METHODS
  Bacterial strains (patient strains and in vitro strains) were supervaccinated on Columbia agar. The bacterial solution was diluted with TSB and aerobically grown, and starting concentration was 1 × 10(7) cfu/ml. The stock solution was placed on Columbia agar. Alginate, polyether, and silicon impression tray adhesives were applied to the center of the particular blood agar plates and incubated for 48 h. The expansion of the inhibition zone assays were measured using a microscope.
  
  
  RESULTS
  Twenty-one different bacterial strains were selected in the saliva samples of 20 patients. The growth inhibition for alginate impression tray adhesive was 1.1 % (±0.3) of the patient strains. The overgrowth of polyether impression tray adhesive was 30.6 % (±9.3) and for silicon impression tray adhesive 11.8 % (±5.0). In in vitro strains, alginate impression tray adhesive performed an inhibition of 0.7 % (±0.3). The overgrowth of polyether impression tray adhesive was 7.0 % (±1.6) and for silicon impression tray adhesive was 6.5 % (±1.3).
  
  
  CONCLUSIONS
  Using the fixed brush for application of the impression tray adhesive on multiple patients, a cross-contamination cannot be ruled out.
  
  
  CONCLUSIONS
  An application of the impression tray adhesive with a pipette and a single-use brush would eliminate the contamination.
authors.affiliation: Department of Prosthodontics, University of Halle, Große Steinstraße 19, 06108 Halle, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Bensel
authors.forename: Rita
authors.initials: R
authors.lastname: Pollak
authors.forename: Michael
authors.initials: M
authors.lastname: Stimmelmayr
authors.forename: Jeremias
authors.initials: J
authors.lastname: Hey
mesh_terms: D000269:Adhesives / Q000737:chemistry*; D000464:Alginates / Q000737:chemistry; D001419:Bacteria / Q000254:growth & development; D001431:Bacteriological Techniques; D003352:Corynebacterium / Q000254:growth & development; D003760:Dental Impression Materials / Q000737:chemistry; D003761:Dental Impression Technique / Q000295:instrumentation*; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination; D004926:Escherichia coli / Q000254:growth & development; D004987:Ethers / Q000737:chemistry; D005260:Female; D006801:Humans; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000254:growth & development; D008875:Middle Aged; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D012463:Saliva / Q000382:microbiology; D017655:Silicon Compounds / Q000737:chemistry; D013210:Staphylococcus / Q000254:growth & development; D013211:Staphylococcus aureus / Q000254:growth & development; D013291:Streptococcus / Q000254:growth & development; D013295:Streptococcus mutans / Q000254:growth & development; D013499:Surface Properties
chemical_list: D000269:Adhesives; D000464:Alginates; D003760:Dental Impression Materials; D004987:Ethers; D017655:Silicon Compounds