title: Phytochemical and antimicrobial investigations of stilbenoids and flavonoids isolated from three species of Combretaceae.
abstract: The antimicrobial activity and chemistry of the African Combretaceae has been well studied in recent years. The present study aimed to investigate the phytochemistry and antimicrobial activity of lesser known members of this family viz. C. hereroense, C. apiculatum and C. collinum. Pulverized leaves of C. collinum and C. apiculatum, and the fruit of C. hereroense were extracted with organic solvents and subjected to preparative chromatography. Seventeen phenolic constituents including four phenanthrenes from the fruit of C. hereroense and two known bibenzyls (including a combretastatin) from the leaves of C. collinum were isolated. The compounds were then subsequently tested for their antimicrobial activity against Candida albicans, Mycobacterium fortuitum, Staphylococcus aureus, Escherichia coli and Proteus vulgaris. Pinocembrin showed excellent activity against C. albicans (MIC - 6.25 μg/ml), superior to that of the positive control, fluconazole and against S. aureus (MIC - 12.5 mg/ml). The phenanthrenes (compounds 1, 2, 3 and 5) showed some activity against M. fortuitum and S. aureus with a uniform MIC of 25 μg/ml. From this study it was evident that most stilbenoids and flavonoids from the selected Combretaceae have little or no antimicrobial activity.
authors.affiliation: Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, Scotland, UK. david.katerere@mrc.ac.za
authors.forename: David R
authors.initials: DR
authors.lastname: Katerere
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Gray
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Nash
authors.forename: Roger D
authors.initials: RD
authors.lastname: Waigh
mesh_terms: D000349:Africa; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D029764:Combretaceae / Q000737:chemistry*; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology*; D015725:Fluconazole / Q000494:pharmacology; D005638:Fruit; D008826:Microbial Sensitivity Tests; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D044950:Flavanones; D010616:Phenanthrenes; D010936:Plant Extracts; D013267:Stilbenes; C016063:pinocembrin; D015725:Fluconazole