title: MRT letter: a fast and easy method for general fluorescent staining of cultured cells on transparent or opaque supports.
abstract: Development of cell-based therapy entails the use of different types of materials as support for cultured cells. Some of these materials are opaque. For a general microscope study of cell cultures prepared on transparent supports, Giemsa stain with bright field microscopy is useful. With opaque supports or scaffolds, epifluorescence microscopy is necessary. The method the authors describe uses eosin Y to stain cytoplasm and DAPI to stain nuclei under fluorescence microscopy. This method provides easy and fast fluorescent staining for a general morphological study of cultured cells on transparent or opaque supports.
authors.affiliation: Department of Cell Biology, Histology and Pharmacology, Instituto de Neurociencias de Castilla y León, University of Valladolid, Valladolid, Spain. gayoso@med.uva.es
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Gayoso
mesh_terms: D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D004801:Eosine Yellowish-(YS) / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; D004801:Eosine Yellowish-(YS)