title: Photodynamic therapy using glycol chitosan grafted fullerenes.
abstract: Glycol chitosan (GC)-grafted fullerene (GC-g-C(60)) conjugates were developed for use in photodynamic therapy of tumor cells. GC-g-C(60) was synthesized in anhydrous benzene/dimethylsulfoxide (DMSO) co-solvent via the chemical conjugation of free amine groups of GC to CC double bonds of C(60). The GC-g-C(60) with 5×10(-4) C(60) molecules per one repeating unit of GC was soluble in water. As C(60) molecules conjugated to GC increased to 0.16 molecules per one repeating unit of GC, GC-g-C(60) started to form supramolecular assemblies (∼30 nm) stabilized in phosphate buffer saline (PBS, 150 mM, pH 7.4). Upon 670 nm light illumination, photo-responsive properties of GC-g-C(60) allowed tremendous singlet oxygen generation in tumor cells for super phototoxicity. GC-g-C(60) also showed highly increased tumor accumulation ability for in vivo tumor of KB tumor-bearing nude mice. It is expected that our GC-g-C(60) conjugate may be a good candidate for in vivo photodynamic therapy in various malignant tumor cells.
authors.affiliation: Division of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok 2-dong, Wonmi-gu, Bucheon-si, Gyeonggi-do 420-743, Republic of Korea.
authors.forename: Dong Sup
authors.initials: DS
authors.lastname: Kwag
authors.forename: Nam Muk
authors.initials: NM
authors.lastname: Oh
authors.forename: Young Taik
authors.initials: YT
authors.lastname: Oh
authors.forename: Kyung Taek
authors.initials: KT
authors.lastname: Oh
authors.forename: Yu Seok
authors.initials: YS
authors.lastname: Youn
authors.forename: Eun Seong
authors.initials: ES
authors.lastname: Lee
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan / Q000008:administration & dosage*; D005260:Female; D037741:Fullerenes / Q000008:administration & dosage*; D006801:Humans; D008027:Light; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000188:drug therapy*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage*
chemical_list: D037741:Fullerenes; D017319:Photosensitizing Agents; C118638:glycol-chitosan; D048271:Chitosan