title: Quantification of proteins by functionalized gold nanoparticles using click chemistry.
abstract: This letter presents a click-chemistry-based assay for proteins (CAP) that allows quantitative determination of the concentration of proteins, using azide- and alkyne-functionalized gold nanoparticles (AuNPs). Compared with conventional methods, CAP has a broader linear range for detection of proteins with good selectivity. CAP enables the analysis of total proteins in various sera and milk samples.
authors.forename: Kui
authors.initials: K
authors.lastname: Zhu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Sha
authors.initials: S
authors.lastname: He
authors.forename: Wenwen
authors.initials: W
authors.lastname: Chen
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wang
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000480:Alkynes / Q000737:chemistry; D000818:Animals; D001386:Azides / Q000737:chemistry; D002417:Cattle; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000480:Alkynes; D001386:Azides; D011506:Proteins; D006046:Gold; D003300:Copper