title: Basis for ligand discrimination between ON and OFF state riboswitch conformations: the case of the SAM-I riboswitch.
abstract: Riboswitches are RNA elements that bind to effector ligands and control gene expression. Most consist of two domains. S-Adenosyl Methionine (SAM) binds the aptamer domain of the SAM-I riboswitch and induces conformational changes in the expression domain to form an intrinsic terminator (transcription OFF state). Without SAM the riboswitch forms the transcription ON state, allowing read-through transcription. The mechanistic link between the SAM/aptamer recognition event and subsequent secondary structure rearrangement by the riboswitch is unclear. We probed for those structural features of the Bacillus subtilis yitJ SAM-I riboswitch responsible for discrimination between the ON and OFF states by SAM. We designed SAM-I riboswitch RNA segments forming "hybrid" structures of the ON and OFF states. The choice of segment constrains the formation of a partial P1 helix, characteristic of the OFF state, together with a partial antiterminator (AT) helix, characteristic of the ON state. For most choices of P1 vs. AT helix lengths, SAM binds with micromolar affinity according to equilibrium dialysis. Mutational analysis and in-line probing confirm that the mode of SAM binding by hybrid structures is similar to that of the aptamer. Altogether, binding measurements and in-line probing are consistent with the hypothesis that when SAM is present, stacking interactions with the AT helix stabilize a partially formed P1 helix in the hybrids. Molecular modeling indicates that continuous stacking between the P1 and the AT helices is plausible with SAM bound. Our findings raise the possibility that conformational intermediates may play a role in ligand-induced aptamer folding.
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70802, USA.
authors.forename: Vamsi Krishna
authors.initials: VK
authors.lastname: Boyapati
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Jessica
authors.initials: J
authors.lastname: Spedale
authors.forename: Fareed
authors.initials: F
authors.lastname: Aboul-Ela
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D001412:Bacillus subtilis; D008024:Ligands; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D059370:RNA Folding; D058928:Riboswitch; D012436:S-Adenosylmethionine / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D008024:Ligands; D058928:Riboswitch; D012313:RNA; D012436:S-Adenosylmethionine