title: Chlamydia trachomatis antigens recognized in women with tubal factor infertility, normal fertility, and acute infection.
abstract: OBJECTIVE
  To identify Chlamydia trachomatis antigens associated with tubal factor infertility and acute infection.
  
  
  METHODS
  A C trachomatis proteome array was used to compare antibody profiles among women with tubal factor infertility, normal fertility, and acute C trachomatis infection.
  
  
  RESULTS
  Thirteen immunodominant antigens reacted with 50% or more sera from all women (n=73). Six C trachomatis antigens were uniquely recognized in women with tubal factor infertility. Combining fragmentation of the six antigens with serum sample dilution, chlamydial antigens HSP60, CT376, CT557, and CT443 could discriminate between women with tubal factor infertility and women with normal fertility with a sensitivity of 63% (95% confidence interval [CI] 0.41-0.77) and specificity of 100% (95% CI 0.91-1), respectively. These antigens were designated as tubal factor infertility-associated antigens. However, these tubal factor antigens were unable to distinguish tubal factor infertility patients from those with acute infection. A combination of CT875 and CT147 distinguished women with acute infection from all other C trachomatis-exposed women with a detection sensitivity of 63% (95% CI 0.41-0.77) and specificity of 100% (95% CI 0.95-1), respectively. Thus, CT875 and CT147 were designated as acute infection-associated antigens.
  
  
  CONCLUSIONS
  A sequential screening of antibodies against panels of C trachomatis antigens can be used to identify women with tubal factor infertility and acute C trachomatis infection.
  
  
  METHODS
  II.
authors.affiliation: Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Budrys
authors.forename: Siqi
authors.initials: S
authors.lastname: Gong
authors.forename: Allison K
authors.initials: AK
authors.lastname: Rodgers
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Christopher
authors.initials: C
authors.lastname: Louden
authors.forename: Rochelle
authors.initials: R
authors.lastname: Shain
authors.forename: Robert S
authors.initials: RS
authors.lastname: Schenken
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhong
mesh_terms: D000208:Acute Disease; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers / Q000097:blood; D002690:Chlamydia Infections / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000276:immunology*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D040081:Protein Array Analysis; D020543:Proteome; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D020543:Proteome