title: Microsatellite development for the relictual conifer Araucaria araucana (Araucariaceae) using next-generation sequencing.
abstract: OBJECTIVE
  In this study, the 454 GS-FLX genome sequence system was used for the identification and characterization of microsatellites in Araucaria araucana, one of the most important and endangered species of Chilean and Argentinean native forests.
  
  
  RESULTS
  A total of 35876 reads were identified, 96% of which were within the size range selected for further analysis. Of these, 1563 contained a microsatellite insert suitable for primer design. Ten simple sequence repeat (SSR) markers provided easily interpretable patterns and were used to evaluate the genetic diversity in four populations of the species. The 10 microsatellites showed high polymorphism levels, with a total of 99 alleles and 32 private alleles. The observed heterozygosity was high and ranged from 0.513 to 0.723.
  
  
  CONCLUSIONS
  The primers presented in this study display high genetic diversity and may provide useful information for the design of conservation strategies in Araucaria araucana.
authors.affiliation: Departamento de Genética, Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario (ceiA3), Córdoba, Spain.
authors.forename: M Angela
authors.initials: MA
authors.lastname: Martín
authors.forename: Claudia
authors.initials: C
authors.lastname: Mattioni
authors.forename: Ilaria
authors.initials: I
authors.lastname: Lusini
authors.forename: Fernando
authors.initials: F
authors.lastname: Drake
authors.forename: Marcello
authors.initials: M
authors.lastname: Cherubini
authors.forename: M Angel
authors.initials: MA
authors.lastname: Herrera
authors.forename: Fiorella
authors.initials: F
authors.lastname: Villani
authors.forename: Luis M
authors.initials: LM
authors.lastname: Martín
mesh_terms: D056426:Genetic Loci / Q000235:genetics; D005828:Genetics, Population; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D064028:Tracheophyta / Q000235:genetics*