title: CSMD1 exhibits antitumor activity in A375 melanoma cells through activation of the Smad pathway.
abstract: In this work, we studied the effects of CUB and Sushi multiple domains 1 gene (CSMD1) expression in A375 melanoma cells in vivo and in vitro. CSDM1 expression decreased proliferation and migration, and increased apoptosis and G(1) arrest in A375 cells in vitro. Expression of CSDM1 in established xenografted tumors decreased tumor size and weight, and decreased the density of intratumor microvessels. The survival rate of mice with tumors expressing CSMD1 was significantly higher than mice with tumors that did not express CSDM1. These results confirm the role of CSDM1 as a tumor suppressor gene in melanoma cells. Furthermore, we found that CSMD1 can interact with Smad3, activate Smad1, Smad2, and Smad3, and increase the expression of Smad4. These results might prove helpful for the development of novel therapies for melanoma treatment.
authors.affiliation: Department of Plastic Surgery, The First Hospital of China Medical University, Nanjing North Street 155 Heping District, Shenyang City, 110001, China. doctor_tangmingrui@163.com
authors.forename: Ming-Rui
authors.initials: MR
authors.lastname: Tang
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Wang
authors.forename: Shu
authors.initials: S
authors.lastname: Guo
authors.forename: Si-Yuan
authors.initials: SY
authors.lastname: Han
authors.forename: Di
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic; D015398:Signal Transduction; D051898:Smad1 Protein / Q000235:genetics / Q000378:metabolism*; D051899:Smad2 Protein / Q000235:genetics / Q000378:metabolism*; D051900:Smad3 Protein / Q000235:genetics / Q000378:metabolism*; D051901:Smad4 Protein / Q000096:biosynthesis; D025521:Tumor Suppressor Proteins; D023041:Xenograft Model Antitumor Assays
chemical_list: C477880:CSMD1 protein, human; D008565:Membrane Proteins; C497399:SMAD1 protein, human; C497782:SMAD2 protein, human; C497790:SMAD3 protein, human; C497796:SMAD4 protein, human; D051898:Smad1 Protein; D051899:Smad2 Protein; D051900:Smad3 Protein; D051901:Smad4 Protein; D025521:Tumor Suppressor Proteins