title: Dental pulp stem cells from primary teeth quality analysis: laboratory procedures.
abstract: OBJECTIVE
  To present details of isolation, processing and differentiation of stem cells from inflamed dental pulp of primary teeth.
  
  
  METHODS
  Tissue sample was collected from teeth indicated for a single visit pulp therapy. Samples were transported and processed in the laboratory which included culturing of cells, isolation and in vitro differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow.
  
  
  RESULTS
  There was no statistically significant difference found in the expression of various surface markers between dental pulp and bone marrow. The stem cells from dental pulp were differentiated into multiple lineages.
  
  
  CONCLUSIONS
  Isolation of cells from oral tissues is technique sensitive.
authors.affiliation: Pedodontics & Preventive Dentistry, ITS-CDSR, Muradnagar. rooposhi@yahoo.com
authors.forename: R
authors.initials: R
authors.lastname: Saha
authors.forename: S
authors.initials: S
authors.lastname: Tandon
authors.forename: R
authors.initials: R
authors.lastname: Rajendran
authors.forename: R
authors.initials: R
authors.lastname: Nayak
mesh_terms: D017667:Adipocytes / Q000166:cytology; D053687:Adult Stem Cells / Q000166:cytology*; D015703:Antigens, CD / Q000032:analysis; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D019902:Chondrocytes / Q000166:cytology; D003782:Dental Pulp / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology; D011671:Pulpitis / Q000473:pathology; D014094:Tooth, Deciduous / Q000166:cytology*
chemical_list: D015703:Antigens, CD