title: Involvement of spinal monocyte chemoattractant protein-1 (MCP-1) in cancer-induced bone pain in rats.
abstract: In this study, we examined the involvement of chemokine monocyte chemoattractant protein-1 (MCP-1) in the spinal cord of a rat model of cancer-induced bone pain (CIBP). In this model, CIBP was established by an intramedullary injection of Walker 256 cells into the tibia of rats. We observed a significant increase in expression levels of MCP-1 and its receptor CCR2 in the spinal cord of CIBP rats. Furthermore, the intrathecal administration of an anti-MCP-1 neutralizing antibody attenuated the mechanical allodynia established in CIBP rats. Likewise, an intrathecal injection of exogenous recombinant MCP-1 induced a striking mechanical allodynia in naïve rats. These results suggest that increases in spinal MCP-1 and CCR2 expression are involved in the development of mechanical allodynia associated with bone cancer rats.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Hu
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zheng
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Yang
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Wang
authors.forename: Fu-Hai
authors.initials: FH
authors.lastname: Ji
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism* / Q000556:secondary; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006930:Hyperalgesia / Q000150:complications / Q000628:therapy; D007278:Injections, Spinal; D010146:Pain / Q000209:etiology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D054390:Receptors, CCR2 / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D054390:Receptors, CCR2