title: [DNA mutations associated to rifampicin or isoniazid resistance in M. tuberculosis clinical isolates from Sonora, Mexico].
abstract: OBJECTIVE
  To perform the analysis of specific regions of the major genes associated with resistance to isoniazid or rifampin.
  
  
  METHODS
  Twenty two M. tuberculosis strains, isolated from human samples obtained in Sonora, Mexico. Specific primers for hotspots of the rpoB, katG, inhA genes and the ahpC-oxyR intergenic region were used. The purified PCR products were sequenced.
  
  
  RESULTS
  Mutations in the promoter of inhA, the ahpC-oxyR region, and codon 315 of katG and in 451 or 456 codons of rpoB, were identified.
  
  
  CONCLUSIONS
  Detection of mutations not previously reported requires further genotypic analysis of Mycobacterium tuberculosis isolates in Sonora.
authors.affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, México. ebolado@guayacan.uson.mx
authors.forename: Enrique
authors.initials: E
authors.lastname: Bolado-Martínez
authors.forename: Ansix
authors.initials: A
authors.lastname: Pérez-Mendoza
authors.forename: Francisco Monserrat
authors.initials: FM
authors.lastname: Alegría-Morquecho
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Candia-Plata
authors.forename: María del Rosario
authors.initials: Mdel R
authors.lastname: Aguayo-Verdugo
authors.forename: Gerardo
authors.initials: G
authors.lastname: Alvarez-Hernández
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D005838:Genotype; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008800:Mexico; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D012293:Rifampin / Q000494:pharmacology*
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial; D007538:Isoniazid; D012293:Rifampin