title: Identification of OASL d, a splice variant of human OASL, with antiviral activity.
abstract: The 2',5'-oligoadenylate synthetases (OASs) are IFN-induced antiviral proteins and are upregulated by infection of viral and some bacterial pathogens. There are at least 2 transcripts of approximately 1.8 and 2.0 kb in interferon-beta treated samples that are recognized by a probe for human OASL in Northern blot assay. By RT-PCR amplification we have isolated a previously undescribed splice variant of human OASL, named OASL d. The new variant was derived from deletion of exons 4 and 5 and encodes a protein of 384 aa residues that shares the N-terminal 219 aa residues with OASL a. Sequence analysis indicates that OASL d also contains the entire ubiquitin-like domain identified in human OASL a. OASL d was strongly induced by IFNγ in THP-1 monocytic cells and in A549 epithelial cells by interferon-beta as detected by immunoblotting assay. Ectopic expression of OASL a or OASL d, but not OASL b that shares the N-terminus with OASL a and d, partially inhibited EV71 and VSV infection. No effect against HSV-2 infection was observed. Therefore, OASL d is a novel isoform of human OASL that possesses antiviral activity against RNA viruses.
authors.affiliation: Jiangsu Laboratory of Molecular Medicine, School of Medicine, China.
authors.forename: Xuancheng
authors.initials: X
authors.lastname: Guo
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Xu
authors.forename: Tian
authors.initials: T
authors.lastname: Sun
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wu
authors.forename: Erguang
authors.initials: E
authors.lastname: Li
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D005091:Exons; D017353:Gene Deletion; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology; D020033:Protein Isoforms; D014162:Transfection; D014709:Vero Cells
chemical_list: D020033:Protein Isoforms; D016899:Interferon-beta; C115020:OASL protein, human; D015088:2',5'-Oligoadenylate Synthetase