title: Protective role of simvastatin on isolated rabbit atrioventricular node during experimental atrial fibrillation model: role in rate control of ventricular beats.
abstract: The purpose of the present study was to determine (1) whether simvastatin (SV) modifies the rate-dependent conduction time and refractoriness of the atrioventricular (AV) node and (2) how it can change the protective mechanism of the AV node during atrial fibrillation (AF). Predefined stimulation protocols were applied to detect the electrophysiological parameters of the AV node, including atrial-His conduction time, effective refractory period (ERP), functional refractory period (FRP), concealed conduction, excitable index, and fatigue in two groups of isolated, perfused rabbit AV nodal preparations (N=16). The stimulation protocols (fatigue, recovery) were carried out during control and in the presence of SV (0.5, 0.8, 3, and 10 μM). Simulated AF was executed in a separate group (N=8), and specific indexes, including H-H mean, zone of concealment (ZOC), and concealed beats were recorded. SV, in a concentration-dependent manner, prolonged ERP, FRP, and Wenckebach cycle lengths. It (10 μM) significantly increased fatigue and the excitable index. In addition, SV elicited prolongation of ZOC and H-H mean at 3 and 10 μM. SV-evoked prolongation of nodal refractoriness and concealed conduction caused rate-dependent ventricular slowing effects during AF. The ability of simvastatin to decrease the excitable gap by its heterogeneous effects on nodal dual pathways proposes its protective role in AF.
authors.affiliation: Golestan Cardiovascular research center, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Khori
authors.forename: Soroosh Aminolsharieh
authors.initials: SA
authors.lastname: Najafi
authors.forename: Ali Mohammad
authors.initials: AM
authors.lastname: Alizadeh
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Moheimani
authors.forename: Delaram
authors.initials: D
authors.lastname: Shakiba
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Alizadeh
authors.forename: Mohsen
authors.initials: M
authors.lastname: Nayebpour
mesh_terms: D000818:Animals; D001281:Atrial Fibrillation / Q000503:physiopathology*; D001283:Atrioventricular Node / Q000187:drug effects* / Q000502:physiology; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D006339:Heart Rate / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits; D019821:Simvastatin / Q000494:pharmacology*
chemical_list: D002316:Cardiotonic Agents; D019821:Simvastatin