title: Comparison of five DNA  extraction methods for molecular analysis of Jerusalem artichoke (Helianthus  tuberosus).
abstract: DNA  extraction is an essential step for molecular analysis of an organism, but it  is difficult to acquire a sufficient amount of pure DNA from plant tissue with  high levels of phenolic compounds, carbohydrates, proteins, and secondary  metabolites. Jerusalem artichoke (Helianthus tuberosus) has high levels  of such substances. We compared five commonly used methods of extracting  genomic DNA in tests made with leaves and seed of four Jerusalem artichoke genotypes:  1) modified method of Tai and Tanksley, 2) method of Doyle and Doyle, 3) method  of Porebski, 4) modified method of Štorchová, and 5) Plant DNA Kit of Omega  Bio-tek. The quality and quantity of extracted DNAs were assessed by  photometric assay, electrophoresis on 1% agarose gel and a PCR-based  technique. The modified method of Tai and Tanksley was found to be superior for  both young leaves and seed. The quality of the extracted DNA was confirmed by  sequence-related amplified polymorphism. This information will be useful for  molecular analyses of Jerusalem artichoke and other related Helianthus species.
authors.affiliation: Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: T
authors.initials: T
authors.lastname: Mornkham
authors.forename: P P
authors.initials: PP
authors.lastname: Wangsomnuk
authors.forename: P
authors.initials: P
authors.lastname: Wangsomnuk
authors.forename: S
authors.initials: S
authors.lastname: Jogloy
authors.forename: A
authors.initials: A
authors.lastname: Pattanothai
authors.forename: Y B
authors.initials: YB
authors.lastname: Fu
mesh_terms: D018744:DNA, Plant / Q000302:isolation & purification*; D005821:Genetic Techniques; D006368:Helianthus / Q000737:chemistry / Q000235:genetics*; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics
chemical_list: D018744:DNA, Plant