title: Cyclic stretch stimulates recruitment of active Na⁺/K⁺-ATPase subunits to the plasma membrane of skeletal muscle cells.
abstract: Cyclic stretch increases Na(+)/K(+)-ATPase activity and abundance in several tissues, including skeletal muscle cells. The present study was undertaken to investigate whether Na(+)/K(+)-ATPase undergoes acute changes in its catalytic activity in response to cyclic stretch. Na(+)/K(+)-ATPase activity increased after continuously stretched for 6 h, and reached the maximum at 24 h. The inhibition of gene transcription (actinomycin D) had no effect on stretch-induced Na(+)/K(+)-ATPase activity. Cyclic stretch also increases the plasma membrane content of α(1)- and α(2)-subunit of Na(+)/K(+)-ATPase. Brefeldin A could completely abolished the stretch-induced recruitment of α-subunits to the plasma membrane and Na(+)/K(+)-ATPase activity. In conclusion, cyclic stretch directly stimulates Na(+)/K(+)-ATPase activity in skeletal muscle cells through post-transcriptional activation, likely by increasing translocation of Na(+)/K(+)-ATPase molecules to plasma membrane.
authors.affiliation: Department of Periodontics, College of Stomatology, School of Medicine, Wu Han University, 237 Luoyu Road, Wuhan 430079, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Yan
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.forename: Chengzhang
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D020126:Brefeldin A / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology*; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D018482:Muscle, Skeletal / Q000166:cytology; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D014158:Transcription, Genetic
chemical_list: D021122:Protein Subunits; D020126:Brefeldin A; D000254:Sodium-Potassium-Exchanging ATPase