title: EST-based identification of immune-relevant genes from spleen of Indian catfish, Clarias batrachus (Linnaeus, 1758).
abstract: A normalized cDNA library from spleen of Indian catfish, Clarias batrachus, was constructed with a redundancy factor of 2.29. A total of 2045 clones from the library were single-pass sequenced, which generated 1937 high quality ESTs with an average read length of approximately 700 bp. Based on sequence similarities, 65 ESTs were found to be associated with immune functions, which were mainly associated with response to stress, response to chemical stimulus, cellular response to stimulus, response to external stimulus, immune response and regulation of response to stimulus. The immune-relevant gene for CD141, thrombomodulin, has been identified in Teleosts for the first time. Six EST-SSRs and three SNPs were found associated with eight immune-relevant genes. These markers associated with important immune genes would be useful for the identification of trait associated alleles for marker-assisted selection. The identification of the putative immune-related genes provides a meaningful framework to understand the Indian catfish immune system and defense mechanisms.
authors.affiliation: National Bureau of Fish Genetic Resources, Canal Ring Road, PO Dilkusha, Lucknow-226002 U.P., India.
authors.forename: Akanksha
authors.initials: A
authors.lastname: Singh
authors.forename: Neeraj
authors.initials: N
authors.lastname: Sood
authors.forename: U K
authors.initials: UK
authors.lastname: Chauhan
authors.forename: Vindhya
authors.initials: V
authors.lastname: Mohindra
mesh_terms: D056704:Adaptive Immunity / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D002397:Catfishes / Q000235:genetics* / Q000276:immunology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D020224:Expressed Sequence Tags; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015723:Gene Library; D005819:Genetic Markers; D007113:Immunity, Innate / Q000235:genetics; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000276:immunology / Q000378:metabolism*; D018180:Thrombomodulin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D016207:Cytokines; D029941:Fish Proteins; D005819:Genetic Markers; D018180:Thrombomodulin; D009113:Muramidase