title: Exposure of Staphylococcus aureus to silver(I) induces a short term protective response.
abstract: The Ag(I) ion has well established anti-bacterial and antifungal properties. Exposure of Staphylococcus aureus to MIC(80) AgNO(3) (3 μg/ml) lead to an increase in the activity of superoxide dismutase, glutathione reductase and catalase at 30 min but activity declined by 60 min. In addition, exposure of cells to this metal ion for 1 h lead to increased expression of a number of proteins such as elongation factors Ts, Tu and G, fructose-bisphosphate aldolase and triosephosphate isomerase but their expression declined following 4 h exposure. ATP binding cassette transporter protein and oligoendopeptidase F showed increased expression at 4 h. While Ag(I) is a potent antimicrobial agent this work demonstrates that S. aureus can mount a short-term protective response to exposure to the metal ion but that this is eventually overcome.
authors.affiliation: Medical Mycology Unit, NICB, Department of Biology, National University of Ireland Maynooth, Maynooth, Co. Kildare, Ireland.
authors.forename: Alanna
authors.initials: A
authors.lastname: Smith
authors.forename: Raymond
authors.initials: R
authors.lastname: Rowan
authors.forename: Malachy
authors.initials: M
authors.lastname: McCann
authors.forename: Kevin
authors.initials: K
authors.lastname: Kavanagh
mesh_terms: D002374:Catalase / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D012834:Silver / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D012834:Silver; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005634:Fructose-Bisphosphate Aldolase