title: Electrospinning collagen and hyaluronic acid nanofiber meshes.
abstract: Collagen and hyaluronic acid (HA) are main components of the extracellular matrix and have been utilized in electrospinning; a technique that creates nanosized fibers for tissue scaffolds. A collagen/HA polymer solution was electrospun into a scaffold material for osteoporosis patients who have reduced bone strength. To synthesize nanofibers, a high voltage was applied to the polymer solution to draw out nanofibers that were collected on a ground plate as a uniform mesh. The meshes were then crosslinked to render them insoluble and conjugated with gold nanoparticles to promote biocompatibility. Characterization of the mesh was performed using scanning electron microscope, electron dispersive spectroscopy and fourier transform infrared spectroscopy. A WST-1 assay determined the potential biocompatibility. The results show that collagen/HA scaffolds were developed that were insoluble in aqueous solutions and promoted cellular attachment that could be used as a tissue engineered scaffold to promote cell growth.
authors.affiliation: Department of Biological Engineering, University of Missouri, Room 148 Agricultural Engineering Building, Columbia, MO 65211, USA. rlfwvd@mail.missouri.edu
authors.forename: Rachael L
authors.initials: RL
authors.lastname: Fischer
authors.forename: Michael G
authors.initials: MG
authors.lastname: McCoy
authors.forename: Sheila A
authors.initials: SA
authors.lastname: Grant
mesh_terms: D001672:Biocompatible Materials; D003094:Collagen / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D006820:Hyaluronic Acid; D003094:Collagen