title: The adenovirus E1A N-terminal repression domain represses transcription from a chromatin template in vitro.
abstract: The adenovirus repression domain of E1A 243R at the E1A N-terminus (E1A 1-80) transcriptionally represses genes involved in differentiation and cell cycle progression. E1A 1-80 represses transcription in vitro from naked DNA templates through its interaction with p300 and TFIID. E1A 1-80 can also interact with several chromatin remodeling factors and associates with chromatin in vivo. We show here that E1A 243R and E1A 1-80 can repress transcription from a reconstituted chromatin template in vitro. Temporal analysis reveals strong repression by E1A 1-80 when added at pre-activation, activation and early transcription stages. Interestingly, E1A 1-80 can greatly enhance transcription from chromatin templates, but not from naked DNA, when added at pre-initiation complex (PIC) formation and transcription-initiation stages. These data reveal a new dimension for E1A 1-80's interface with chromatin and may reflect its interaction with key players in PIC formation, p300 and TFIID, and/or possibly a role in chromatin remodeling.
authors.affiliation: St. Louis University School of Medicine, Institute for Molecular Virology, 1100 S. Grand Ave., Saint Louis, MO 63104, USA.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Loewenstein
authors.forename: Shwu-Yuan
authors.initials: SY
authors.lastname: Wu
authors.forename: Cheng-Ming
authors.initials: CM
authors.lastname: Chiang
authors.forename: Maurice
authors.initials: M
authors.lastname: Green
mesh_terms: D017500:Adenovirus E1A Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000258:Adenovirus Infections, Human / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D000260:Adenoviruses, Human / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020816:Amino Acid Motifs; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D006801:Humans; D014158:Transcription, Genetic
chemical_list: D017500:Adenovirus E1A Proteins; D002843:Chromatin