title: [The preparation procedure of tests for the gas chromatographic determination of fat acids without preliminary extraction of lipids].
abstract: The enhancement of the procedure of quantitative gas chromatographic determination of fit acids in biologic liquids samples is proposed. Instead of the conventional Folch procedure of extraction of lipids with subsequent ablution, concentration and methylation of extracts the direct saponification and methylation of vacuum dried liquid samples (50-200 mkl) can be applied. To compare the effectiveness of the proposed and conventional procedures both of them had been applied to evaluate how converge the results of determination of composition of fat acids in whole blood, blood plasma, packed red blood cells, homogenates of hepatic and muscular tissues. The proposed procedure is applied to determine the characteristics of fat acids composition inpatients with ischemic heart disease.
authors.forename: A V
authors.initials: AV
authors.lastname: Aripovskiĭ
authors.forename: P O
authors.initials: PO
authors.lastname: Kolesnik
authors.forename: M I
authors.initials: MI
authors.lastname: Vezhdel
authors.forename: V N
authors.initials: VN
authors.lastname: Titov
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002849:Chromatography, Gas / Q000379:methods; D005227:Fatty Acids / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008745:Methylation; D009203:Myocardial Infarction / Q000378:metabolism
chemical_list: D005227:Fatty Acids