title: Graphene oxide-based biosensor for sensitive fluorescence detection of DNA based on exonuclease III-aided signal amplification.
abstract: Based on the super fluorescence quenching efficiency of graphene oxide and exonuclease III aided signal amplification, we develop a facile, sensitive, rapid and cost-effective method for DNA detection. In the presence of target DNA, the target-probe hybridization forms a double-stranded structure and exonuclease III catalyzes the stepwise removal of mononucleotides from the blunt 3' termini of probe, resulting in the recycling of the target DNA and signal amplification. Therefore, our proposed sensor exhibits a high sensitivity towards target DNA with a detection limit of 20 pM, which was even lower than previously reported GO-based DNA sensors without enzymatic amplification, and provides a universal sensing platform for sensitive detection of DNA.
authors.affiliation: School of Pharmacology, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, PR China.
authors.forename: Xu-Hua
authors.initials: XH
authors.lastname: Zhao
authors.forename: Qiu-Juan
authors.initials: QJ
authors.lastname: Ma
authors.forename: Xiang-Xiang
authors.initials: XX
authors.lastname: Wu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhu
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D055664:Electrochemical Techniques; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005453:Fluorescence; D006108:Graphite / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D010087:Oxides; D006108:Graphite; D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III