title: A PR-1-like protein of Fusarium oxysporum functions in virulence on mammalian hosts.
abstract: The pathogenesis-related PR-1-like protein family comprises secreted proteins from the animal, plant, and fungal kingdoms whose biological function remains poorly understood. Here we have characterized a PR-1-like protein, Fpr1, from Fusarium oxysporum, an ubiquitous fungal pathogen that causes vascular wilt disease on a wide range of plant species and can produce life-threatening infections in immunocompromised humans. Fpr1 is secreted and proteolytically processed by the fungus. The fpr1 gene is required for virulence in a disseminated immunodepressed mouse model, and its function depends on the integrity of the proposed active site of PR-1-like proteins. Fpr1 belongs to a gene family that has expanded in plant pathogenic Sordariomycetes. These results suggest that secreted PR-1-like proteins play important roles in fungal pathogenicity.
authors.affiliation: Departamento de Genetica, Facultad de Ciencias and Campus de Excelencia Internacional Agroalimentario ceiA3, Universidad de Cordoba, 14071 Cordoba, Spain.
authors.forename: Rafael C
authors.initials: RC
authors.lastname: Prados-Rosales
authors.forename: Raquel
authors.initials: R
authors.lastname: Roldán-Rodríguez
authors.forename: Carolina
authors.initials: C
authors.lastname: Serena
authors.forename: Manuel S
authors.initials: MS
authors.lastname: López-Berges
authors.forename: Josep
authors.initials: J
authors.lastname: Guarro
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Martínez-del-Pozo
authors.forename: Antonio
authors.initials: A
authors.lastname: Di Pietro
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D005670:Fusarium / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D020935:MAP Kinase Signaling System; D051379:Mice; D008957:Models, Genetic; D009696:Nucleic Acids / Q000737:chemistry; D010802:Phylogeny; D010843:Pichia / Q000378:metabolism; D010944:Plants / Q000382:microbiology; D010957:Plasmids / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D015398:Signal Transduction; D014774:Virulence; D037521:Virulence Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: C575023:Fpr1 protein, Fusarium oxysporum; D005656:Fungal Proteins; D009696:Nucleic Acids; D011994:Recombinant Proteins; D037521:Virulence Factors; D005947:Glucose