title: Solubility evaluation of murine hybridoma antibodies.
abstract: The successful development of antibody therapeutics depends on the molecules having properties that are suitable for manufacturing, as well as use by patients. Because high solubility is a desirable property for antibodies, screening for solubility has become an essential step during the early candidate selection process. In considering the screening process, we formed a hypothesis that hybridoma antibodies are filtered by nature to possess high solubility and tested this hypothesis using a large number of murine hybridoma-derived antibodies. Using the cross-interaction chromatography (CIC) method, we screened the solubility of 92 murine hybridoma-derived monoclonal antibodies and found that all of these molecules exhibited CIC profiles that are indicative of high solubility (> 100mg/mL). Further investigations revealed that variable region N-linked glycosylation or isoelectric parameters are unlikely to contribute to the high solubility of these antibodies. These results support the general hypothesis that hybridoma monoclonal antibodies are highly soluble.
authors.affiliation: Biotechnology Center of Excellence, Janssen Research & Development, LLC, Radnor, PA, USA.
authors.forename: Stacey
authors.initials: S
authors.lastname: Spencer
authors.forename: Deidra
authors.initials: D
authors.lastname: Bethea
authors.forename: T Shantha
authors.initials: TS
authors.lastname: Raju
authors.forename: Jill
authors.initials: J
authors.lastname: Giles-Komar
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000302:isolation & purification; D006825:Hybridomas / Q000378:metabolism*; D007163:Immunosorbent Techniques; D007525:Isoelectric Focusing; D051379:Mice; D012995:Solubility
chemical_list: D000911:Antibodies, Monoclonal