title: Trichoderma biodiversity in China.
abstract: In the present study, we made further investigation into the diversity of Trichoderma in China than previous ones utilizing comprehensive approaches of morphological microscopic observation and phylogenetic analysis by detecting molecular markers. One thousand nine hundred ten Trichoderma strains were isolated from soil or other materials in China: East (Anhui, Fujian, Jiangsu, Jiangxi, Shandong, Zhejiang province and Shanghai municipality), South-West (Guizhou, Qinghai, Shanxi, Sichuan and Yunnan province, Tibet Autonomous Region and Chongqing municipality), South-East (Guangdong, Guangxi, Hainan province), and Middle China (Henan, Hubei and Hunan province). Representative isolates were verified at the species level by morphological characters and the oligonucleotide barcode program TrichoOKey v.10 and the custom BLAST server TrichoBLAST, using sequence of the ITS 1 and 2 region of the rDNA cluster and partial sequences of translation elongation factor 1-alpha(tef1-α). A total of 23 Trichoderma species were identified : T.asperellum, T.atrioviride, T.aureovriride, T.brevicompactum, T.citrioviride, T.erinaceum, T.gamsii, T.hamatum, T.harzianum (H.1ixii), T.intricatum, T.koningii (H.koningii), T.koningiopsis, T.longibranchiatum, T.pleuroticola, T.reeseii (H.jecorina), T.sinensis, T.spirale, T.stromaticum, T.tomentosum, T.velutinum, T.vermipilum, T.virens (H.virens), T.viride. Among them, 3 species: T.intricatum, T.stromaticum, T.vermipilum were first reported in China; T.harzianum (H,1ixii) was the most widely distributed species in China. This study further shows that, the highest biodiversity of Trichoderma population appeared in South-West China.
authors.affiliation: Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Rui-yan
authors.initials: RY
authors.lastname: Sun
authors.forename: Zhi-cheng
authors.initials: ZC
authors.lastname: Liu
authors.forename: Kehe
authors.initials: K
authors.lastname: Fu
authors.forename: Lili
authors.initials: L
authors.lastname: Fan
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D044822:Biodiversity; D002681:China; D062305:Geographic Mapping; D010802:Phylogeny; D013045:Species Specificity; D014242:Trichoderma / Q000235:genetics*