title: Anti-inflammatory effects of Amomum compactum on RAW 264.7 cells via induction of heme oxygenase-1.
abstract: Amomum compactum is commonly used in Korean traditional medicine. In this study, we demonstrate that A. compactum ethanolic extract (ACEE) has anti-inflammatory effects in a lipopolysaccharide-induced RAW 264.7 cell model of inflammation. In this system, ACEE prominently inhibited the production of nitric oxide (NO), prostaglandin E(2) (PGE(2)), interleukin (IL)-6 and tumor necrosis factor (TNF)-α, and inhibited the protein expression of inducible nitric oxide synthase and cyclooxygenase-2. Furthermore, ACEE treatment inhibited the translocation of nuclear factor-kappaB (NF-κB) and the degradation of inhibitory factor-kappaB alpha, but enhanced the expression of heme oxygenase (HO)-1 and the nuclear translocation of nuclear factor-erythroid 2 (Nrf2). Treatment with tin protoporphyrin IX dichloride (SnPP), a selective HO-1 inhibitor, reversed the ACEE-induced suppression of NO production, suggesting that the induction of HO-1 is involved in the suppression of NO, TNF-α, and IL-6 production by ACEE. Taken together, these results suggest that ACEE have anti-inflammatory effects occurring through HO-1 induction, which leads to suppression of the blocking NF-κB.
authors.affiliation: Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, Daejeon, 305-811, Korea.
authors.forename: Jin-Ah
authors.initials: JA
authors.lastname: Lee
authors.forename: Mee-Young
authors.initials: MY
authors.lastname: Lee
authors.forename: In-Sik
authors.initials: IS
authors.lastname: Shin
authors.forename: Chang-Seob
authors.initials: CS
authors.lastname: Seo
authors.forename: HyeKyung
authors.initials: H
authors.lastname: Ha
authors.forename: Hyeun Kyoo
authors.initials: HK
authors.lastname: Shin
mesh_terms: D035801:Amomum / Q000737:chemistry*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000037:antagonists & inhibitors; D004790:Enzyme Induction; D005455:Fluorescent Antibody Technique; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D018613:Microscopy, Confocal; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors; D000093242:Interleukin-6 Inhibitors
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D051547:Heme Oxygenase-1; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D016328:NF-kappa B; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors; C496317:Nos2 protein, mouse; C496542:Hmox1 protein, mouse