title: Transcriptional regulation of the genes encoding chitin and β-1,3-glucan synthases from Ustilago maydis.
abstract: Transcriptional regulation of genes encoding chitin synthases (CHS) and β-1,3-glucan synthase (GLS) from Ustilago maydis was studied. Transcript levels were measured during the growth curve of yeast and mycelial forms, in response to ionic and osmotic stress, and during infection of maize plants. Expression of the single GLS gene was constitutive. In contrast, CHS genes expression showed differences depending on environmental conditions. Transcript levels were slightly higher in the mycelial forms, the highest levels occurring at the log phase. Ionic and osmotic stress induced alterations in the expression of CHS genes, but not following a defined pattern, some genes were induced and others repressed by the tested compounds. Changes in transcripts were more apparent during the pathogenic process. At early infection stages, only CHS6 gene showed significant transcript levels, whereas at the period of tumor formation CHS7 and CHS8 genes were also were induced.
authors.affiliation: Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, GTO, Mexico.
authors.forename: Mariana
authors.initials: M
authors.lastname: Robledo-Briones
authors.forename: José
authors.initials: J
authors.lastname: Ruiz-Herrera
mesh_terms: D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D014158:Transcription, Genetic; D014588:Ustilago / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000382:microbiology; D047071:beta-Glucans / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D047071:beta-Glucans; C033363:beta-1,3-glucan; D005964:Glucosyltransferases; C040232:glucan synthase