title: A phytochemical approach to experimental metabolic syndrome-associated renal damage and oxidative stress.
abstract: The aim of this study was to evaluate the effect of DTS-phytocompound on oxidant-antioxidant balance and protein damage in the kidneys of rats administered high doses of fructose. Adult male Wistar rats were divided into four groups. Group A received a control diet, whereas groups B and C were fed a high-fructose diet (60 g/100 g), the latter with additional DTS (50 mg/kg per day) for 60 days. Lipo- and nitro-peroxidation together with α-smooth muscle actin (α-SMA) expression in the glomerular and interstitial tissue of the kidneys were measured after 60 days. Fructose-fed rats showed significantly higher lipoperoxidation, 2,4-dinitrophenol and 3-nitrotyrosine protein adducts, and upregulation of α-SMA in the kidney. DTS significantly decreased such redox unbalance in renal tissue, while partially downregulating α-SMA (p<0.01). These data suggest the potential clinical benefit of DTS in protecting the kidneys from metabolic syndrome-associated changes; gender-related analysis is under way.
authors.affiliation: ReGenera Res Group for Aging Intervention, Milan, Italy. fmarchimede@libero.it
authors.forename: Francesco
authors.initials: F
authors.lastname: Marotta
authors.forename: Archana
authors.initials: A
authors.lastname: Kumari
authors.forename: Roberto
authors.initials: R
authors.lastname: Catanzaro
authors.forename: Umberto
authors.initials: U
authors.lastname: Solimene
authors.forename: Shalini
authors.initials: S
authors.lastname: Jain
authors.forename: Emilio
authors.initials: E
authors.lastname: Minelli
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Harada
mesh_terms: D019297:2,4-Dinitrophenol / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D007668:Kidney / Q000196:embryology; D007674:Kidney Diseases / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D024821:Metabolic Syndrome / Q000150:complications / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D008517:Phytotherapy / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005632:Fructose; C002744:3-nitrotyrosine; D014443:Tyrosine; D019297:2,4-Dinitrophenol