title: [Postcolostral immunoglobulin G (IgG) levels in newborn piglets using three different ELISA detection systems].
abstract: Objective  of the study was to evaluate whether the Colostrum Quality Counter (CQC), a new test method for immunoglobulin G (IgG) levels in newborn piglets, is easy to handle and provides comparable results to established testing regimes.
  
  
  METHODS
  Blood samples from 219 piglets from four different farms were tested for their IgG-concentrations using three different ELISA tests. Furthermore, double samples from 30 piglets were taken from both the anterior vena cava and from the tail to determine whether the collection site affects the results. The three tests used were the Colostrum Quality Counter (CQC; FarmulaONE, NL-Best), the internal IgG-ELISA from our laboratory (MUC) and a commercially-available IgG-ELISA (NAT; NatuTec, Frankfurt/Main, Germany).
  
  
  RESULTS
  MUC and NAT showed a higher correlation to each other than to the CQC when referring to the individual results per single piglet. The results from the CQC were higher and the standard deviation was significantly greater. The sampling site had no significant effect on the IgG concentrations measured.
  
  
  CONCLUSIONS
  The CQC is a straightforward and simple test, being very convenient for sampling a large number of piglets. CQC results were inhomogeneous with some unusually high IgG-concentrations. MUC and NAT provided comparable results to one another and the IgG-concentrations showed a good correlation.
authors.affiliation: Lehrstuhl für Tierschutz, Verhaltenskunde, Tierhygiene und Tierhaltung, Tierärztliche Fakultät der Ludwig-Maximilians-Universität München. pausiemausie@arcor.de
authors.forename: A
authors.initials: A
authors.lastname: Pausenberger
authors.forename: K
authors.initials: K
authors.lastname: Schuster
authors.forename: M H
authors.initials: MH
authors.lastname: Erhard
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D001800:Blood Specimen Collection / Q000379:methods* / Q000592:standards; D001835:Body Weight; D003126:Colostrum / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005260:Female; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D013552:Swine / Q000097:blood / Q000276:immunology*; D013623:Tail / Q000098:blood supply; D014682:Vena Cava, Inferior
chemical_list: D007074:Immunoglobulin G