title: [Effects of advanced glycosylation end products and tetrandrine on proliferation of K562 and K562/A02 cells].
abstract: This study was aimed to investigate the effect of advanced glycosylation end products (AGE) on the proliferation of K562 and K562/A02 cells, the effect of tetrandrine (Tet) on proliferation of K562 and K562/A02 cells induced by AGE, and their mechanisms. The effects of AGE on proliferation of K562 and K562/A02 cells and Tet on the proliferation of AGE-induced K562 and K562/A02 cells were assayed by CCK8 kit, the apoptosis rate and the expression of receptor of advanced glycosylation end products (RAGE) in K562 and K562/A02 cells were determined by flow cytometry, the expression of RAGE mRNA was detected by semi-quantitative RT-PCR. The results showed that AGE could promote the proliferation of K562 and K562/A02 cells in a concentration-dependent manner, the cell proliferation was enhanced with time increasing in 0 - 48 h, and was higher than control group after 72 h. AGE up-regulated the RAGE mRNA and protein expressions of K562 and K562/A02 cells in a concentration-dependent manner. Treatment of Tet combined with AGE for 48 h could inhibit the proliferation of K562 and K562/A02 cells promoted by AGE in a concentration-dependent manner, which probably by inducing cell apoptosis, however, there was no obvious effect in the up-regulating expression of RAGE mRNA and protein induced by AGE. It is concluded that AGE can promote the proliferation of K562 and K562/A02 cells, which is probably induced by up-regulating the expression of RAGE mRNA and protein. Tet can inhibit the proliferation of K562 and K562/A02 cells induced by AGE, and the mechanism may be not closely associated with changes of the up-regulating expression of RAGE mRNA and protein induced by AGE.
authors.affiliation: Department of Hematology and Oncology, Southeast University Medical College, Jiangsu Province, China.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Yan
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Xia
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Cheng
authors.forename: Jia-Hua
authors.initials: JH
authors.lastname: Ding
authors.forename: Wen
authors.initials: W
authors.lastname: Bao
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D044182:Benzylisoquinolines / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D015973:Gene Expression Regulation, Leukemic; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D000067759:Receptor for Advanced Glycation End Products / Q000378:metabolism
chemical_list: D044182:Benzylisoquinolines; D017127:Glycation End Products, Advanced; D000067759:Receptor for Advanced Glycation End Products; C009438:tetrandrine