title: Monitoring microcirculation changes in port wine stains during vascular targeted photodynamic therapy by laser speckle imaging.
abstract: This study was conducted to test laser speckle perfusion imaging (LSPI) for imaging microcirculation and monitoring microcirculatory changes of port wine stains (PWS) during vascular targeted photodynamic therapy (V-PDT). Before and 5 min after V-PDT, PWS lesions and the corresponding contralateral healthy skins of 24 PWS patients were scanned, whereas seven PWS patients were scanned throughout V-PDT. V-PDT was conducted immediately after intravenous injection of photocarcinorin (4-5 mg kg(-1)). A 532 nm laser was used for irradiation (power density: 80-100 mW cm(-2), exposure time: 20-50 min). Before V-PDT, all 24 PWS patients demonstrated a significant difference in perfusion between the PWS lesion and the contralateral healthy control skin (1132 ± 724 and 619 ± 478 PU, respectively, P < 0.01). Five minutes after V-PDT, the mean perfusion value of the 24 PWS lesions was 1246 ± 754 PU. There was no significant difference compared to the perfusion before V-PDT (P > 0.05). During V-PDT, the perfusion of seven PWS patients increased rapidly after initiation of V-PDT, reached a maximum within 10 min, lasted for several minutes, and slowly returned to a relatively lower level at the end of V-PDT. On the basis of these results, LSPI is capable of imaging PWS microvasculature and monitoring microvascular reactivity to V-PDT.
authors.affiliation: Department of Laser Medicine, Chinese PLA General Hospital, Beijing, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Qiu
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
authors.forename: Qing
authors.initials: Q
authors.lastname: Ang
authors.forename: ShiYong
authors.initials: S
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Zeng
authors.forename: Naiyan
authors.initials: N
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D006415:Hematoporphyrins / Q000008:administration & dosage; D006801:Humans; D007834:Lasers; D008027:Light; D008297:Male; D008833:Microcirculation / Q000528:radiation effects*; D055806:Microvessels / Q000473:pathology / Q000528:radiation effects*; D008875:Middle Aged; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage; D019339:Port-Wine Stain / Q000188:drug therapy* / Q000473:pathology; D012867:Skin / Q000098:blood supply / Q000473:pathology / Q000528:radiation effects*
chemical_list: D006415:Hematoporphyrins; D017319:Photosensitizing Agents; C052552:photocarcinorin