title: DNA-templated silver nanoclusters-graphene oxide nanohybrid materials: a platform for label-free and sensitive fluorescence turn-on detection of multiple nucleic acid targets.
abstract: In this study, we develop an efficient method for multiple DNA detection by exploring silver nanoclusters (AgNCs)-graphene oxide (GO) nanohybrid materials. Because of the extraordinarily high quenching efficiency of GO, the ssDNA-AgNCs probe exhibits minimal background fluorescence, while strong emission is observed when it forms a double helix with the specific target DNA, leading to a high signal-to-background ratio. Therefore the AgNCs-GO nanohybrid materials can be successfully applied for DNA detection. The system described here exhibits not only high sensitivity with a detection limit of 1 nM, but also an excellent differentiation ability for single-base mismatched sequences. In addition, by exploring AgNCs as signal reporters and GO as the nanoquencher, this approach avoids labeling the probe DNA or target DNA, which offers the advantages of simplicity and cost efficiency. Moreover, the large planar surface of GO allows adsorption of different DNA-AgNCs probes, each with a distinct emission, leading to a multicolor sensor for the detection of multiple DNA targets in the same solution.
authors.affiliation: Laboratory of Chemical Biology, Graduate School of the Chinese Academy of Sciences, Changchun Institute of Applied Chemistry, Jilin, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tao
authors.forename: Youhui
authors.initials: Y
authors.lastname: Lin
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Huang
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010087:Oxides / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D015342:DNA Probes; D010087:Oxides; D012834:Silver; D006108:Graphite; D004247:DNA