title: Pretreatment with anti-flagellin serum delays acute lung injury in rats with sepsis.
abstract: OBJECTIVE
  This study examined the reduction of sepsis-induced ALI by inhibition of flagellin-stimulated TLR5 signaling.
  
  
  METHODS
  Rats were randomly divided into three groups: one group served as the sham-operated group (control group), and the other two groups received the induction of sepsis (sepsis and treatment groups). The treatment group was injected with anti-flagellin serum before induction of sepsis. At 2, 4, 6, 12, 24, and 48 h following induction of sepsis (six time-point subgroups, n = 10 per subgroup), arterial PaO(2), wet/dry (W/D) lung weight ratios, levels of serum and BALF flagellin and TNF-α, pulmonary pathological alterations, and TLR5 mRNA expression in the lungs were examined.
  
  
  RESULTS
  Compared to sham-operated rats, septic rats had: increased levels of serum and BALF flagellin at 6, 12, 24, and 48 h; reduced arterial PaO(2); elevated W/D lung weight ratio; increased serum and BALF TNF-α levels; and up-regulated TLR5 mRNA expression at 12, 24, and 48 h (P < 0.01). Pretreatment with anti-flagellin serum, however, significantly inhibited sepsis-associated declines in arterial PaO(2), increased W/D lung weight ratios, elevated serum and BALF TNF-α levels, and up-regulated TLR5 mRNA expression at 24 and 48 h (P < 0.01).
  
  
  CONCLUSIONS
  Neutralizing the actions of circulating flagellin with anti-flagellin serum delayed the development of ALI in rats with sepsis.
authors.affiliation: Institute of Respiratory Diseases of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing, China. huhanshandd@yahoo.com.cn
authors.forename: Ming-Dong
authors.initials: MD
authors.lastname: Hu
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Chang-Xi
authors.initials: CX
authors.lastname: Zhou
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Wang
authors.initials: W
authors.lastname: Yi
authors.forename: Gui-Sheng
authors.initials: GS
authors.lastname: Qian
authors.forename: Mei
authors.initials: M
authors.lastname: Mao
authors.forename: Jian-Cheng
authors.initials: JC
authors.lastname: Xu
mesh_terms: D055371:Acute Lung Injury / Q000097:blood / Q000276:immunology* / Q000473:pathology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D005408:Flagellin / Q000097:blood / Q000276:immunology*; D008297:Male; D012333:RNA, Messenger / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000097:blood / Q000276:immunology* / Q000473:pathology; D044967:Serum; D051198:Toll-Like Receptor 5 / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000276:immunology
chemical_list: D012333:RNA, Messenger; D051198:Toll-Like Receptor 5; D014409:Tumor Necrosis Factor-alpha; D005408:Flagellin