title: Isolation of heat-tolerant myoglobin from Asian swamp eel Monopterus albus.
abstract: Myoglobin from Asian swamp eel Monopterus albus was purified from fish muscle using salt fractionation followed by column chromatography and molecular filtration. The purified Mb of 0.68 mg/g wet weight of muscle was determined for its molecular mass by MALDI-TOF-MS to be 15,525.18 Da. Using isoelectric focusing technique, the purified Mb showed two derivatives with pI of 6.40 and 7.12. Six peptide fragments of this protein identified by LC-MS/MS were homologous to Mbs of sea raven Hemitripterus americanus, yellowfin tuna Thunnus albacores, blue marlin Makaira nigicans, common carp Cyprinus carpio, and goldfish Carassius auratus. According to the Mb denaturation, the swamp eel Mb had thermal stability higher than walking catfish Clarias batrachus Mb and striped catfish Pangasius hypophthalmus Mb, between 30 and 60 (°)C. For the thermal stability of Mb, the swamp eel Mb showed a biphasic behavior due to the O(2) dissociation and the heme orientation disorder, with the lowest increase in both Kd(f) and Kd(s). The thermal sensitivity of swamp eel Mb was lower than those of the other Mbs for both of fast and slow reaction stages. These results suggest that the swamp eel Mb globin structure is thermally stable, which is consistent with heat-tolerant behavior of the swamp eel particularly in drought habitat.
authors.affiliation: Department of Chemistry, Faculty of Science, Center of Excellence for Innovation in Chemistry, Khon Kaen University, 123 Mittrapab Road, T. Ni-Muang, A. Muang, Khon Kaen, 40002, Thailand. chot.chaya@gmail.com
authors.forename: Chatrachatchaya
authors.initials: C
authors.lastname: Chotichayapong
authors.forename: Kittipong
authors.initials: K
authors.lastname: Wiengsamut
authors.forename: Saksit
authors.initials: S
authors.lastname: Chanthai
authors.forename: Nison
authors.initials: N
authors.lastname: Sattayasai
authors.forename: Toru
authors.initials: T
authors.lastname: Tamiya
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kanzawa
authors.forename: Takahide
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005591:Chemical Fractionation; D002845:Chromatography / Q000379:methods; D004524:Eels / Q000378:metabolism*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000502:physiology; D009211:Myoglobin / Q000737:chemistry* / Q000378:metabolism*; D055550:Protein Stability; D013050:Spectrometry, Fluorescence
chemical_list: D009211:Myoglobin