title: Kinetics of advanced glycation end products formation on bovine serum albumin with various reducing sugars and dicarbonyl compounds in equimolar ratios.
abstract: Reducing sugars and reactive dicarbonyl compounds play a major role in glycation of proteins in vivo. Glycation of proteins is the first step in of a nonenzymatic reaction, resulting in advanced glycation end products (AGEs). AGEs can inactivate proteins or modify their biological activities. Therefore, it is important to understand the mechanism of AGE formation. Here, we systematically analyzed the kinetics of AGE formation in vitro by fluorescence and absorption measurements utilizing a microplate reader system and bovine serum albumin (BSA) as a model protein. Comparing different concentrations of BSA, we applied various reducing sugars and reactive dicarbonyl compounds as AGE-inducing agents at different concentrations. In summary, this experimental setup enabled us to measure the kinetics of AGE formation in an efficient and defined way.
authors.affiliation: Institut für Physikalische Biologie, Heinrich-Heine-Universität, Dusseldorf, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Luers
authors.forename: Karolina
authors.initials: K
authors.lastname: Rysiewski
authors.forename: Christian
authors.initials: C
authors.lastname: Dumpitak
authors.forename: Eva
authors.initials: E
authors.lastname: Birkmann
mesh_terms: D000042:Absorption; D000818:Animals; D001120:Arginine / Q000737:chemistry / Q000378:metabolism; D002241:Carbohydrates / Q000737:chemistry*; D002417:Cattle; D005632:Fructose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D019636:Neurodegenerative Diseases / Q000378:metabolism; D012266:Ribose / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D016622:Silver Staining; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors
chemical_list: D002241:Carbohydrates; D017127:Glycation End Products, Advanced; D012710:Serum Albumin, Bovine; D005632:Fructose; D012266:Ribose; D001120:Arginine; D005947:Glucose