title: Tumor and non-tumor liver angiogenesis is traced and evaluated by hepatic arterial ultrasound in murine models.
abstract: We studied the relationships between hepatic and mesenteric mean blood-flow velocities (mBFVs) measured by ultrasound imaging and (1) downstream tumor angiogenesis during liver metastasis induced by spleen injection of LS174 human colon cells overexpressing the antiangiogenic Netrin4 (LS174-NT4) or not (LS174-WT) and (2) downstream normal angiogenesis during hepatic regeneration after 50% hepatectomy. Liver volume and mBFVs were measured before and after surgery, at day 30 in the first model and at days 2, 7 and 16 in the second model. LS174-NT-4 vs. LS174-WT mice presented fewer metastases (25% vs. 90%, p < 0.001) and decreased hepatic mBFVs (16.5 ± 0.8 vs. 21.8 ± 1.4 cm s(-1), p < 0.01), without difference in mesenteric mBFVs. After partial hepatectomy, hepatic and mesenteric mBFVs increased at day 7, from 12.4 ± 1.7 and 11.8 ± 2.6 to 19.1 ± 1.8 and 17.5 ± 2.4 cm s(-1), respectively, (p < 0.01) then returned to baseline as liver volume. Duplex Doppler ultrasonography reliably assesses normal or tumor angiogenesis and may provide follow-up functional evaluation.
authors.affiliation: Université Paris-Diderot, Sorbonne Paris Cité, INSERM, UMR-S 965, Paris, France.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Eveno
authors.forename: Carole
authors.initials: C
authors.lastname: Le Henaff
authors.forename: Raphaëlle
authors.initials: R
authors.lastname: Audollent
authors.forename: Philippe
authors.initials: P
authors.lastname: Soyer
authors.forename: Aurore
authors.initials: A
authors.lastname: Rampanou
authors.forename: Judith
authors.initials: J
authors.lastname: Nemeth
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Brouland
authors.forename: Evelyne
authors.initials: E
authors.lastname: Dupuy
authors.forename: Marc
authors.initials: M
authors.lastname: Pocard
authors.forename: Philippe
authors.initials: P
authors.lastname: Bonnin
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D006499:Hepatic Artery / Q000000981:diagnostic imaging*; D006801:Humans; D008113:Liver Neoplasms / Q000150:complications* / Q000000981:diagnostic imaging*; D051379:Mice; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014463:Ultrasonography