title: A three-stage culture process for improved exopolysaccharide production by Tremella fuciformis.
abstract: Tremella fuciformis produces several bioactive secondary metabolites including exopolysaccharides. Cultivation of the fungus was carried out in a three-stage process consisting of a 1.5-day cultivation with orbital shaking at 200 rpm, a 1.5-day cultivation with reciprocal shaking at 200 strokes, and a 1.5-day cultivation with orbital shaking at 200 rpm. Exopolysaccharide production and specific production rate reached 5.80 g L(-1) and 0.15 d(-1), respectively, which is an increase of 260% and 200% compared with the corresponding values for fermentations with orbital shaking only, and of 243% and 150% compared with the corresponding values for fermentations with reciprocal shaking only. The three-stage culture method is time-saving and easy to operate.
authors.affiliation: Center for Bioengineering and Biotechnology, China University of Petroleum, East China, Qingdao 266555, PR China. zhuhu@upc.edu.cn
authors.forename: Hu
authors.initials: H
authors.lastname: Zhu
authors.forename: Baozhen
authors.initials: B
authors.lastname: Tian
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Shuaishuai
authors.initials: S
authors.lastname: Zhang
authors.forename: Chunxu
authors.initials: C
authors.lastname: Cao
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Weisheng
authors.initials: W
authors.lastname: Zou
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D005285:Fermentation / Q000502:physiology; D011134:Polysaccharides / Q000096:biosynthesis*; D013172:Spores, Fungal / Q000378:metabolism
chemical_list: D011134:Polysaccharides