title: Modulation of immune response by interleukin-10 in systemic Corynebacterium kutscheri infection in mice.
abstract: Interleukin (IL)-10 is an anti-inflammatory cytokine that modulates sepsis by decreasing pro-inflammatory cytokine production and chemokine expression. In this study, IL-10-deficient and wild-type (WT) mice were infected with Corynebacterium kutscheri to determine if the absence of IL-10 altered the protective immunity and pathogenesis. After infection, IL-10 knockout (KO) mice had a higher survival rate than WT mice. The decrease of body weight and the increased weight of organs such as liver and spleen were greater in WT mice. Bacterial counts were significantly increased after inoculation in WT mice over those in IL-10 KO mice. WT mice had more granulomatous inflammation and coagulative necrosis in the liver and spleen, lymphocyte depletion in lymphoid follicles, and apoptosis of immune cells in the spleen. WT mice had significantly higher plasma concentrations of aspartate aminotransferase and alanine aminotransferase. Furthermore, more upregulation of tumor necrosis factor-α and IL-4 in the plasma, macrophage inflammatory protein-2, keratinocyte-derived chemokine, inducible nitric oxide synthase, and interferon-inducible protein 10 mRNA in the spleen were observed in WT mice after inoculation. These results suggest that the lack of IL-10 contributes to an increase in the systemic clearance of C. kutscheri, and that IL-10 plays a detrimental role in controlling systemic C. kutscheri infection.
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, 143-701, Republic of Korea.
authors.forename: Eui-Suk
authors.initials: ES
authors.lastname: Jeong
authors.forename: Kyoung-Sun
authors.initials: KS
authors.lastname: Lee
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Heo
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Seo
authors.forename: Yang-Kyu
authors.initials: YK
authors.lastname: Choi
mesh_terms: D000818:Animals; D003352:Corynebacterium / Q000276:immunology / Q000502:physiology*; D003354:Corynebacterium Infections / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology*; D008099:Liver / Q000276:immunology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D013154:Spleen / Q000276:immunology
chemical_list: D016753:Interleukin-10