title: The effect of Cu(II)-loaded brushite scaffolds on growth and activity of osteoblastic cells.
abstract: Bone substitute materials such as calcium phosphate cements (CPC) are frequently used as growth factor carriers for the stimulation of osteoblast-formation around an implant. However, biological modification based on delicate protein factors like extracellular matrix proteins or growth factors is subject to a number of shortcomings like the need for storage below room temperature and cost of production. The aim of this study was to investigate ionic modification as an alternative bioinorganic route for implant modification. Although it is known that Cu(II) plays a role in angiogenesis and bone formation, not all involved processes are well understood yet. In this study the in vitro effect of Cu(II) on growth and activity of osteoblastic cells seeded on brushite (CaHPO(4) · 2 H(2) O) scaffolds as well as on glass discs was investigated. The results show that Cu(II) enhances cell activity and proliferation of osteoblastic cells on CPC and furthermore affects the expression of several bone specific proteins such as bone sialo protein or osteocalcin. Therefore, the modification of CPC with Cu(II) may offer a promising alternative to protein based modification to stimulate cellular activity for an improved bone healing.
authors.affiliation: Department for Functional Materials in Medicine and Dentistry, Dental School, University of Würzburg, Würzburg, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ewald
authors.forename: Christine
authors.initials: C
authors.lastname: Käppel
authors.forename: Elke
authors.initials: E
authors.lastname: Vorndran
authors.forename: Claus
authors.initials: C
authors.lastname: Moseke
authors.forename: Michael
authors.initials: M
authors.lastname: Gelinsky
authors.forename: Uwe
authors.initials: U
authors.lastname: Gbureck
mesh_terms: D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003300:Copper / Q000008:administration & dosage* / Q000494:pharmacology*; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002130:Calcium Phosphates; D015675:Osteocalcin; D003300:Copper; C494366:calcium phosphate, dibasic, dihydrate