title: Isolation, molecular characterization, and phylogenetic analysis of porcine encephalomyocarditis virus strain HB10 in China.
abstract: Encephalomyocarditis virus (EMCV) can cause myocarditis, respiratory failure, reproductive failure, and mortality in pregnant sows, fetuses, and ablactating piglets. Diseases caused by EMCV currently affect the swine industry worldwide. A virus was isolated from organs of dead piglets that presented with acute myocarditis in northern China. The production of a specific cytopathic effect on susceptible cells and the results of hemagglutination inhibition (HI) assay, PCR, electron microscopy (EM), and sequencing indicated that the pathogen was EMCV; the strain was named HB10. Other pathogenic agents causing myocarditis and death were excluded as possible pathogenic agents. Phylogenetic analyses of the capsid coding region and the VP3/VP1 genes using the neighbour-joining method revealed that EMCV isolates cluster into two groups (groups 1 and 2) with two sub-clusters within group 1 (group 1a and b). HB10 belongs to group 1a, along with strains CBNU, GX0601, BJC3, NJ08, and BEL-2887A/91. Five strains isolated from Sus scrofa belong to group 2. The results of this and previous studies indicate that HB10 and other EMCV strains cause myocarditis of pigs in China.
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, Heilongjiang, China. liuyebing@ivdc.gov.cn
authors.forename: Wencheng
authors.initials: W
authors.lastname: Lin
authors.forename: Yebing
authors.initials: Y
authors.lastname: Liu
authors.forename: Shangjin
authors.initials: S
authors.lastname: Cui
authors.forename: Hongmei
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D018188:Cardiovirus Infections / Q000662:veterinary* / Q000821:virology; D002681:China; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000821:virology*