title: Recent advances in sulfotransferase enzyme activity assays.
abstract: Sulfotransferases are enzymes that catalyze the transfer of sulfo groups from a donor, for example 3'-phosphoadenosine 5'-phosphosulfate, to an acceptor, for example the amino or hydroxyl groups of a small molecule, xenobiotic, carbohydrate, or peptide. These enzymes are important targets in the design of novel therapeutics for treatment of a variety of diseases. This review examines assays used for this important class of enzyme, paying particular attention to sulfotransferases acting on carbohydrates and peptides and the major challenges associated with their analysis.
authors.affiliation: Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Paul
authors.forename: Jiraporn
authors.initials: J
authors.lastname: Suwan
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Dordick
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D005470:Fluorometry; D013058:Mass Spectrometry; D011874:Radiometry; D015238:Sulfotransferases / Q000378:metabolism*
chemical_list: D015238:Sulfotransferases