title: Quantitative phase contrast optimised cancerous cell differentiation via ptychography.
abstract: This paper shows that visible-light ptychography can be used to distinguish quantitatively between healthy and tumorous unstained cells. Advantages of ptychography in comparison to conventional phase-sensitive imaging techniques are highlighted. A novel procedure to automatically refocus ptychographic reconstructions is also presented, which improves quantitative analysis.
authors.affiliation: Kroto Research Institute, University of Sheffield,S3 7HQ, Sheffield, UK. d.claus@sheffield.ac.uk
authors.forename: Daniel
authors.initials: D
authors.lastname: Claus
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Maiden
authors.forename: Fucai
authors.initials: F
authors.lastname: Zhang
authors.forename: Francis G R
authors.initials: FG
authors.lastname: Sweeney
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Humphry
authors.forename: Hermann
authors.initials: H
authors.lastname: Schluesener
authors.forename: John M
authors.initials: JM
authors.lastname: Rodenburg
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D003937:Diagnosis, Differential; D005910:Glioma / Q000473:pathology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D051381:Rats; D012031:Refractometry / Q000379:methods*