title: Preparation of carboxyl group-modified palladium nanoparticles in an aqueous solution and their conjugation with DNA.
abstract: The use of nanomaterials in biomolecular labeling and their corresponding detection has been attracting much attention, recently. There are currently very few studies on palladium nanoparticles (Pd NPs) due to their lack of appropriate surface functionalities for conjugation with DNA. In this paper, we thus firstly present an approach to prepare carboxyl group-modified Pd NPs (with an average size of 6 nm) by the use of 11-mercaptoundecanoic acid (MUDA) as a stabilizer in the aqueous solution. The effect of the various reducing reaction conditions on the morphology of the Pd NPs was investigated. The particles were further characterized by TEM, UV-vis, FT-IR and XPS techniques. DNA was finally covalently conjugated to the surface of the Pd NPs through the activation of the carboxyl group, which was confirmed by agarose gel electrophoresis and fluorescence analysis. The resulting Pd NPs-DNA conjugates show high single base pair mismatch discrimination capabilities. This work therefore sets a good foundation for further applications of Pd NPs in bio-analytical research.
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China. zfwang@seu.edu.cn
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Wang
authors.forename: Hongying
authors.initials: H
authors.lastname: Li
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhen
authors.forename: Nongyue
authors.initials: N
authors.lastname: He
mesh_terms: D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D020411:Oligonucleotide Array Sequence Analysis; D010165:Palladium / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: C505222:11-mercaptoundecanoic acid; D005227:Fatty Acids; D013438:Sulfhydryl Compounds; D014867:Water; D010165:Palladium; D004247:DNA