title: Toward creating cell membrane glyco-landscapes with glycan lipid constructs.
abstract: Synthetic glycolipid-like constructs dispersible in biological media and capable of incorporating into cell membranes have the ability to create novel artificial glyco-landscapes on living cells. Using a variety of different glycans ranging from disaccharides to polysaccharides, together with different lengths and high hydrophilicity spacers, we created a series of synthetic glycolipid-like constructs. Contacting these constructs with live cells gave modified cells with controlled glycan density and/or altered biological function. The ability to also use these constructs as solutions to inhibit antibodies, toxins, and virions extends the potential diagnostic and therapeutic uses for these synthetic glycolipid-like constructs.
authors.affiliation: Shemyakin Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Korchagina
authors.forename: Alexander
authors.initials: A
authors.lastname: Tuzikov
authors.forename: Andrey
authors.initials: A
authors.lastname: Formanovsky
authors.forename: Inna
authors.initials: I
authors.lastname: Popova
authors.forename: Stephen
authors.initials: S
authors.lastname: Henry
authors.forename: Nicolai
authors.initials: N
authors.lastname: Bovin
mesh_terms: D000017:ABO Blood-Group System; D000906:Antibodies / Q000737:chemistry; D019921:Biotinylation; D002236:Carbohydrate Conformation; D060846:Cell Engineering / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry*; D004912:Erythrocytes / Q000737:chemistry*; D006017:Glycolipids / Q000138:chemical synthesis*; D006031:Glycosylation; D006386:Hemagglutination Tests; D006801:Humans; D009980:Influenza A virus / Q000737:chemistry; D009981:Influenza B virus / Q000737:chemistry; D009439:Neuraminidase / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry; D025942:Oligosaccharides, Branched-Chain; D010714:Phosphatidylethanolamines / Q000737:chemistry*
chemical_list: C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D000017:ABO Blood-Group System; D000906:Antibodies; D006017:Glycolipids; D009844:Oligosaccharides; D025942:Oligosaccharides, Branched-Chain; D010714:Phosphatidylethanolamines; C101335:blood group A trisaccharide; D009439:Neuraminidase