title: [Screening and identification of genes associated with multi-drug resistance in colonic cancer].
abstract: OBJECTIVE
  To identify novel multi-drug resistance-related genes, and to explore the mechanisms of multi-drug resistance.
  
  
  METHODS
  Multi-drug resistant cell line Lovo/5-FU was established by incubation with increasing dose of 5-FU. The sensitivity to 5-FU and cis-diaminodichloroplatinum (CDDP) was measured by MTT assay. Two dimensional electrophoresis plus mass spectrum(2-DE/MS) was used to identify the differentially expressed protein between Lovo and Lovo/5-FU. The identified protein was then verified by Western blot analysis.
  
  
  RESULTS
  The IC50 concentrations of Lovo/5-FU to 5-FU and CDDP were increased by 31 and 3 times, compared with Lovo (both P<0.01). 2DE-MS showed that CAP-G and RhoGDI2 were up-regulated, whereas 6-PGL, DCI, Prdx-6 and Maspin were down-regulated in Lovo/5-FU. Western blot analysis confirmed that the expression levels of RhoGDI2 and CAP-G in Lovo/5-FU were increased by 6.14 and 2.98 fold respectively (both P<0.01), whereas Maspin was decreased to 5.2% of Lovo(P<0.01).
  
  
  CONCLUSIONS
  Multi-gene and multi-pathway are involved in the development of multi-drug resistance of colorectal cancer cells. CAP-G, RhoGDI2 and Maspin are potential multi-drug resistant genes.
authors.affiliation: Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai.
authors.forename: Jian-fang
authors.initials: JF
authors.lastname: Li
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zheng
authors.forename: Bei-qin
authors.initials: BQ
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Qu
authors.forename: Zheng-gang
authors.initials: ZG
authors.lastname: Zhu
authors.forename: Bing-ya
authors.initials: BY
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D015843:Serpins / Q000235:genetics; D062949:rho Guanine Nucleotide Dissociation Inhibitor beta / Q000235:genetics
chemical_list: C569658:ARHGDIB protein, human; D008840:Microfilament Proteins; D009687:Nuclear Proteins; C085344:SERPIN-B5; D015843:Serpins; D062949:rho Guanine Nucleotide Dissociation Inhibitor beta; C066575:CAPG protein, human