title: Peripheral leukocytapheresis attenuates acute lung injury induced by lipopolysaccharide in vivo.
abstract: The mortality of acute lung injury and acute respiratory distress syndrome (ALI/ARDS) remains high and efforts for prevention and treatments have shown little improvement over the past decades. The present study investigated the efficacy and mechanism of leukocytapheresis (LCAP) to partially eliminate peripheral neutrophils and attenuate lipopolysaccharide (LPS)-induced lung injury in dogs. A total of 24 healthy male mongrel dogs were enrolled and randomly divided into LPS, LCAP and LCAP-sham groups. All animals were injected with LPS to induce endotoxemia. The serum levels of leucocytes, neutrophil elastase, arterial blood gas, nuclear factor-kappa B (NF-κB) subunit p65 in lung tissues were measured. The histopathology and parenchyma apoptosis of lung tissues were examined. We found that 7, 3, and 7 animals in the LPS, LCAP, and sham-LCAP groups, respectively, developed ALI 36 h after LPS infusion. The levels of NF-κB p65 in lung tissue, neutrophils and elastase in blood, decreased significantly following LCAP. LCAP also alleviated apoptosis, and NF-κB p65 in lung tissues. Collectively, our results show that partial removal of leucocytes from peripheral blood decreases elastase level in serum. This, in turn, attenuates lung injuries and may potentially decrease the incidence of ALI.
authors.affiliation: Trauma Center & ICU, Daping Hospital & Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China.
authors.forename: Zhi-Gao
authors.initials: ZG
authors.lastname: He
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Shun-Gang
authors.initials: SG
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.forename: Fang-Xiang
authors.initials: FX
authors.lastname: Chen
authors.forename: Xian-Kai
authors.initials: XK
authors.lastname: Huang
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D001784:Blood Gas Analysis; D001992:Bronchoalveolar Lavage Fluid; D004285:Dogs; D019446:Endotoxemia / Q000378:metabolism; D007937:Leukapheresis / Q000379:methods*; D019272:Leukocyte Elastase / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D008954:Models, Biological; D016328:NF-kappa B / Q000378:metabolism; D011897:Random Allocation; D012128:Respiratory Distress Syndrome / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; D019272:Leukocyte Elastase