title: Deferoxamine attenuates iron-induced long-term neurotoxicity in rats with traumatic brain injury.
abstract: This study investigated whether deferoxamine (DFO), an iron chelator attenuates iron-induced toxicity in rats with traumatic brain injury. In this study, three groups of Sprague-Dawley rats (sham, injury and DFO groups) were examined. Rats were killed on day 28 after Morris water maze testing and brains perfused for either non-heme brain binding or hemosiderin staining. Western blotting was used to measure protein levels of ferritin, transferrin and transient receptor potential canonical channel 6 (TRPC6). In TBI rats, there was a significant increase in brain iron on day 28, ferritin L, ferritin H, transferrin and TRPC6 levels were all significantly elevated post-TB1. There were also deficits in spatial learning and memory; however, DFO administration attenuated these effects in TBI rats supporting the notion that DFO may reduce brain injury accentuated by iron overload.
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Gaotanyan 30, Chongqing 400038, People's Republic of China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Meng
authors.forename: Gang
authors.initials: G
authors.lastname: Zhu
authors.forename: Jiangkai
authors.initials: J
authors.lastname: Lin
authors.forename: Hua
authors.initials: H
authors.lastname: Feng
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001052:Apoferritins / Q000378:metabolism; D001284:Atrophy / Q000188:drug therapy / Q000209:etiology; D001921:Brain / Q000187:drug effects / Q000473:pathology; D001930:Brain Injuries / Q000150:complications* / Q000188:drug therapy; D003676:Deferoxamine / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D007501:Iron / Q000633:toxicity*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D020258:Neurotoxicity Syndromes / Q000188:drug therapy* / Q000209:etiology* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017262:Siderophores / Q000627:therapeutic use*; D050052:TRPC Cation Channels / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism
chemical_list: D017262:Siderophores; D050052:TRPC Cation Channels; D014168:Transferrin; C492145:Trpc6 protein, rat; D001052:Apoferritins; D007501:Iron; D003676:Deferoxamine