title: Agitation during lipoplex formation harmonizes the interaction of siRNA to cationic liposomes.
abstract: We recently demonstrated that agitation during lipoplex formation (vorLTsiR) improves the gene knockdown effect of siRNA because the resultant decrease in lipoplex size leads to an enhanced uptake by cells. In furthering this line of research, the present study was focused on the interaction of siRNA to cationic liposomes during lipoplex preparation. A fluorescence resonance energy transfer (FRET) study indicated that the application of agitation in the presence of siRNA effectively reorganized positively charged lipids (DC-6-14 and DOPE) in an order that effectively promoted further electrostatic interaction between the negatively charged phosphate backbone of siRNA and the positively charged lipids in the cationic liposome membrane. A circular dichroism (CD) study indicated that the agitation did not bring about a change in the A-form helix of siRNA, therefore the interactions between the lateral anionic groups of siRNA - responsible for the characteristic bands of the A-form helix - and cationic liposomes were effectively promoted. Factorial design coupled with response surface methodology was used to statistically analyze the influence of vortex speed and time and siRNA dose on the in vitro gene knockdown effects of siRNA-lipoplex that were spontaneously formulated (spoLTsiR) along with that formulated under agitation (vorLTsiR). The analysis indicated that vortex speed plays the most important role in enhancing the gene knockdown effect of siRNA among the three variables, although all three are important. It was concluded that the high energy transmitted by applying agitation during lipoplex formation harmonized the interaction of siRNA to positively charged lipids (DC-6-14 and DOPE) in cationic liposomes, resulting in a superior gene knockdown efficacy of vorLTsiR compared to spoLTsiR. Our study suggests that the preparation procedure is one of the critical factors in producing the enhanced gene knockdown effect of siRNA.
authors.affiliation: Department of Pharmacokinetics and Biopharmaceutics, Institute of Health Biosciences, The University of Tokushima, 1-78-1 Sho-machi, Tokushima, Japan.
authors.forename: José Mario
authors.initials: JM
authors.lastname: Barichello
authors.forename: Shinji
authors.initials: S
authors.lastname: Kizuki
authors.forename: Tatsuaki
authors.initials: T
authors.lastname: Tagami
authors.forename: Luiz Alberto Lira
authors.initials: LA
authors.lastname: Soares
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Ishida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kikuchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiwada
mesh_terms: D002412:Cations; D002942:Circular Dichroism; D004983:Ethanolamines / Q000737:chemistry*; D005163:Factor Analysis, Statistical; D031541:Fluorescence Resonance Energy Transfer; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D009038:Motion; D009226:Myristates / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection / Q000379:methods*
chemical_list: D002412:Cations; D004983:Ethanolamines; D008081:Liposomes; D009226:Myristates; C478049:O,O'-detetradecanoyl-N-(alpha-trimethylammonioacetyl)diethanolamine chloride; D010714:Phosphatidylethanolamines; D034741:RNA, Small Interfering; C094877:dioleoyl phosphatidylethanolamine; D008156:Luciferases