title: Almost all human gastric mucin O-glycans harbor blood group A, B or H antigens and are potential binding sites for Helicobacter pylori.
abstract: Helicobacter pylori infects more than half of the world's population. Although most patients are asymptomatic, persistent infection may cause chronic gastritis and gastric cancer. Adhesion of the bacteria to the gastric mucosa is a necessary prerequisite for the pathogenesis of H. pylori-related diseases and is mediated by mucin O-glycans. In order to define which glycans may be implicated in the binding of the bacteria to the gastric mucosa in humans, we have characterized the exact pattern of glycosylation of gastric mucins. We have identified that the major component was always a core 2-based glycan carrying two blood group H antigens, whatever was the blood group of individuals. We have also demonstrated that around 80% of O-glycans carried blood group A, B or H antigens, suggesting that the variation of gastric mucin glycosylation between individuals is partly due to the blood group status. This study will help better understanding the role of O-glycans in the physiology and homeostasis of gastric mucosa. Overall, the results reported here give us the necessary background information to begin studies to determine whether individuals who express certain carbohydrate epitopes on specific mucins are predisposed to certain gastric diseases.
authors.affiliation: Univ Lille Nord de France, F-59000 Lille,  France.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Rossez
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Maes
authors.forename: Tony
authors.initials: T
authors.lastname: Lefebvre Darroman
authors.forename: Pierre
authors.initials: P
authors.lastname: Gosset
authors.forename: Chantal
authors.initials: C
authors.lastname: Ecobichon
authors.forename: Marie
authors.initials: M
authors.lastname: Joncquel Chevalier Curt
authors.forename: Ivo G
authors.initials: IG
authors.lastname: Boneca
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Michalski
authors.forename: Catherine
authors.initials: C
authors.lastname: Robbe-Masselot
mesh_terms: D000017:ABO Blood-Group System / Q000737:chemistry* / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D001665:Binding Sites; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004198:Disease Susceptibility; D005260:Female; D005752:Gastric Mucins / Q000737:chemistry* / Q000378:metabolism; D005753:Gastric Mucosa / Q000737:chemistry* / Q000378:metabolism / Q000382:microbiology; D006031:Glycosylation; D016481:Helicobacter Infections / Q000378:metabolism / Q000382:microbiology; D016480:Helicobacter pylori / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding
chemical_list: D000017:ABO Blood-Group System; D005752:Gastric Mucins; D007983:Lewis Blood Group Antigens; D011134:Polysaccharides