title: Saikosaponin-d inhibits β-conglycinin induced activation of rat basophilic leukemia-2H3 cells.
abstract: β-Conglycinin is one of the major storage proteins in soybean and has been identified as a potential diagnostic marker for severe allergic reactions to soybean. Unfortunately, there is a lack of information on the signal transduction pathways of β-conglycinin induced mast cell activation and how to alleviate these allergic reactions. Bupleurum falcatum, a traditional oriental medicine, has been widely utilized in the treatment of influenza, fever, malaria and menstrual disorders. Furthermore, it has been reported that saikosaponins, the important principle of B. falcatum, possesses anti-allergic activities. Therefore, the present study investigated whether or not saikosaponin-d, an extract of B. falcatum, was effective in the treatment of allergic reactions cased by β-conglycinin, using a rat basophilic leukemia-2H3 cell line. There were multiple signaling pathways contributing to the development of β-conglycinin-mediated rat basophilic leukemia-2H3 cell activation. The intracellular calcium mobilization and tyrosine phosphorylation were early events, which in turn elicited reactive oxygen species production, gene activation of Cdc42 and c-Fos, and ultimately led to β-hexosaminidase release. Saikosaponin-d inhibited rat basophilic leukemia-2H3 cell degranulation by suppressing these critical incidents in the signal transduction pathway. These results suggest that saikosaponin-d exhibited anti-allergic activity and could become an effective herbal therapy for alleviating soybean allergy.
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Hao
authors.forename: Xiangshu
authors.initials: X
authors.lastname: Piao
authors.forename: Xianglan
authors.initials: X
authors.lastname: Piao
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology; D052179:Antigens, Plant / Q000276:immunology*; D020013:Calcium Signaling / Q000187:drug effects; D015550:Cell Degranulation / Q000187:drug effects / Q000276:immunology; D045744:Cell Line, Tumor; D005512:Food Hypersensitivity / Q000188:drug therapy / Q000276:immunology; D005916:Globulins / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D015471:Leukemia, Basophilic, Acute / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology* / Q000502:physiology; D008516:Medicine, Chinese Traditional; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology*; D055314:Seed Storage Proteins / Q000276:immunology*; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D030262:Soybean Proteins / Q000276:immunology*; D013025:Glycine max / Q000009:adverse effects / Q000276:immunology; D014443:Tyrosine / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D052179:Antigens, Plant; D005916:Globulins; D017382:Reactive Oxygen Species; D012503:Saponins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D007073:Immunoglobulin E; D014443:Tyrosine; D009828:Oleanolic Acid; D001619:beta-N-Acetylhexosaminidases; C025759:saikosaponin D