title: Organization and roles of nucleosomes at mouse meiotic recombination hotspots.
abstract: Meiotic double strand breaks (DSBs) occur at discrete regions in the genome coined hotspots. Precisely what directs site selection of these DSBs is hotly debated and in particular it is unclear which chromatin features, and regulatory factors are necessary for a genomic region to initiate and resolve DSBs as a crossover (CO) event. In human and mouse, one layer of hotspot selection control is a recognition sequence element present at these sites that is bound by the Prdm9 zinc-finger protein. Furthermore, an overall open chromatin structure is thought to be required to allow access of the recombination machinery, and this is often dictated by the packaging of DNA around nucleosomes. We recently defined the nucleosome occupancy maps of four mouse recombination hotspots throughout meiosis. These analyses revealed no obvious dynamic changes in nucleosome occupancy, suggesting an intrinsic nature of recombinogenic sites, yet they also revealed that nucleosomes define zones of exclusion for CO resolution. Here, we discuss new evidence implicating nucleosome occupancy in recombinogenic repair and its potential roles in controlling chromatin structure at mouse meiotic hotspots.
authors.affiliation: Genome Plasticity Laboratory, The Department of Cancer Biology, The Scripps Research Institute, Scripps Florida, Jupiter, FL, USA. igetun@scripps.edu
authors.forename: Irina V
authors.initials: IV
authors.lastname: Getun
authors.forename: Zhen K
authors.initials: ZK
authors.lastname: Wu
authors.forename: Philippe R J
authors.initials: PR
authors.lastname: Bois
mesh_terms: D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D008540:Meiosis; D051379:Mice; D009707:Nucleosomes / Q000378:metabolism*; D059372:Nucleotide Motifs; D059767:Recombinational DNA Repair
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D011495:Histone-Lysine N-Methyltransferase; C534829:prdm9 protein, mouse