title: Effect of liposome-encapsulated nisin and bacteriocin-like substance P34 on Listeria monocytogenes growth in Minas frescal cheese.
abstract: The efficacy of liposome-encapsulated nisin and bacteriocin-like substance (BLS) P34 to control growth of Listeria monocytogenes in Minas frescal cheese was investigated. Nisin and BLS P34 were encapsulated in partially purified soybean phosphatidylcholine (PC-1) and PC-1-cholesterol (7:3) liposomes. PC-1 nanovesicles were previously characterized. PC-1-cholesterol encapsulated nisin and BLS P34 presented, respectively, 218 nm and 158 nm diameters, zeta potential of -64 mV and -53 mV, and entrapment efficiency of 88.9% and 100%. All treatments reduced the population of L. monocytogenes compared to the control during 21 days of storage of Minas frescal cheese at 7°C. However, nisin and BLS P34 encapsulated in PC-1-cholesterol liposomes were less efficient in controlling L. monocytogenes growth in comparison with free and PC-1 liposome-encapsulated bacteriocins. The highest inhibitory effect was observed for nisin and BLS P34 encapsulated in PC-1 liposomes after 10 days of storage of the product. The encapsulation of bacteriocins in liposomes of partially purified soybean phosphatidylcholine may be a promising technology for the control of foodborne pathogens in cheeses.
authors.affiliation: Laboratório de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Patrícia da Silva
authors.initials: Pda S
authors.lastname: Malheiros
authors.forename: Voltaire
authors.initials: V
authors.lastname: Sant'Anna
authors.forename: Matheus de Souza
authors.initials: Mde S
authors.lastname: Barbosa
authors.forename: Adriano
authors.initials: A
authors.lastname: Brandelli
authors.forename: Bernadette Dora Gombossy de Melo
authors.initials: BD
authors.lastname: Franco
mesh_terms: D001430:Bacteriocins / Q000494:pharmacology*; D002611:Cheese / Q000382:microbiology*; D005506:Food Contamination / Q000517:prevention & control*; D008081:Liposomes / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development*; D009561:Nisin / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000737:chemistry; D013025:Glycine max
chemical_list: D001430:Bacteriocins; D008081:Liposomes; D010713:Phosphatidylcholines; D009561:Nisin