title: CD25 is a novel marker of hepatic bile canaliculus.
abstract: OBJECTIVE
  Although many antigens have been investigated, the method for the bile canaliculus staining using optical microscopy needs to be improved. The aim of the present study was to assess the expression pattern of a candidate marker, CD25, in normal and diseased liver tissue.
  
  
  METHODS
  Immunohistochemistry, immunofluorescence, and immune electron microscopy assays were performed with 41 liver sections and 2 different anti-CD25 monoclonal antibodies. A polyclonal antibody against carcinoembryonic antigen (CEA) was also used to stain bile canaliculus as a control. CD25 expression levels in normal and diseased liver tissue were also determined.
  
  
  RESULTS
  CD25 was predominantly localized at the bile canaliculus of adult and infantile liver, evidenced by both immunohistochemistry and immunofluorescence assays. The electron microscopy assay showed that there were obvious amorphous electron-dense deposits at the bile canaliculus. In contrast, the CEA-positive area included bile canaliculus as well as basolateral aspects of hepatocytes. CD25 expression levels did not differ significantly among different disease states.
  
  
  CONCLUSIONS
  This study provides the first evidence that CD25 is a novel marker of bile canaliculus. Characteristics of CD25 expression may shed light on immunohistochemistry and immunofluorescence analysis of bile canaliculus in both basic and clinical hepatic investigations.
authors.affiliation: 1Hepatology Research Institute, Contagious Hospital, Wuxi, Jiangsu Province, China.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: -Chen
authors.forename: Chao-Xia
authors.initials: CX
authors.lastname: Ju
authors.forename: Jun
authors.initials: J
authors.lastname: -Den
authors.forename: Shou-gang
authors.initials: SG
authors.lastname: Kuai
authors.forename: Hao
authors.initials: H
authors.lastname: Pei
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Huang
authors.forename: Xi-Bing
authors.initials: XB
authors.lastname: Gu
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Ying
authors.forename: Xi-Qi
authors.initials: XQ
authors.lastname: Hu
authors.forename: Teng-Fang
authors.initials: TF
authors.lastname: Zhu
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Xing
authors.forename: Qin-He
authors.initials: QH
authors.lastname: Fan
mesh_terms: D001648:Bile Canaliculi / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006084:Graft Rejection / Q000378:metabolism / Q000473:pathology; D006509:Hepatitis B / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D008105:Liver Cirrhosis, Biliary / Q000378:metabolism / Q000473:pathology; D008107:Liver Diseases / Q000378:metabolism / Q000473:pathology; D016031:Liver Transplantation; D016253:Microscopy, Immunoelectron / Q000379:methods
chemical_list: D015415:Biomarkers; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit