title: Protective role of Shenfu on ischemia-reperfusion injury of rat liver grafts.
abstract: OBJECTIVE
  We sought to investigate the protective role of Shenfu (SF),a traditional Chinese formulation comprising Radix Ginseng and Radix Aconitum Carmichaeli on ischemia-reperfusion injury in rat liver grafts.
  
  
  METHODS
  Ninety-six male Sprague Dawley rats were used as donors (n = 48) and recipients (n = 48) of orthotopic liver transplantation. They were randomly divided into a control group with donor livers injected with saline through the portal vein immediately after recovery versus the SF group, with livers injected with SF. Each group was further divided into 3 subgroups equally to obtain bood and hepatic tissues samples at 2, 4, and 6 hours reperfusion.
  
  
  RESULTS
  At each phase, the SF group, showed significantly higher bile production (P < .05) with lower serum levels of alanine aminotransferase and tumor necrosis factor-α, and nuclear factor-κB expression in the hepatic tissues. (P < .05). SF group hepatic tissues showed less injury compared with controls.
  
  
  CONCLUSIONS
  SF injection seemed to protect hepatocytes from injury during the early reperfusion phase and to improve subsequent rat liver graft function.
authors.affiliation: Department of general surgery, Yong Chuan affiliated hospital of Chongqing Medical University, Chongqing, PR. China.
authors.forename: T
authors.initials: T
authors.lastname: Chen
authors.forename: M
authors.initials: M
authors.lastname: Cheng
authors.forename: Z
authors.initials: Z
authors.lastname: Yuan
authors.forename: S
authors.initials: S
authors.lastname: Zhou
authors.forename: Z
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001646:Bile / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D019610:Cytoprotection; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008099:Liver / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D008107:Liver Diseases / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D020011:Protective Agents; C492793:Shen-Fu; D014409:Tumor Necrosis Factor-alpha; D000410:Alanine Transaminase