title: Analysis of alphalactalbumin and betalactoglobulin from the rehydration of bovine colostrum powder using cloud point extraction and mass spectrometry.
abstract: Alphalactalbumin (α-La) and betalactoglobulin (β-Lg) in the rehydration of bovine colostrum powder were successfully separated by cloud point extraction using a nonionic surfactant Triton X-114. The effects of different factors, including the surfactant concentration, sample volume, electrolyte, and pH were discussed. The optimized conditions for cloud point extraction of alphalactalbumin (α-La) and betalactoglobulin (β-Lg) can be concluded that the best surfactant is 1% (w/v) Triton X-114, 200 μL of sample volume, 150 mmol/L NaCl, and 6% (w/v) sucrose. After cloud point extraction, the capillary electrophoresis is used to check the efficiency of the extraction procedure. The results had been effectively confirmed by the characterization with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
authors.affiliation: Chinese Academy of Inspection and Quarantine, A3, North Gaobeidian Road, Chaoyang District, Beijing 100123, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Qi
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Zou
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Li