title: Hypertension strengthens δ-ALA-D activity inhibition and increases it reactivation index in type 2 diabetic patients.
abstract: OBJECTIVE
  To assess the effect of hypertension on δ-aminolevulinate dehydratase (δ-ALA-D) activity of type 2 diabetic patients (T2DM).
  
  
  METHODS
  δ-ALA-D activity and reactivation index, as well as markers of oxidative stress, biochemical and anthropometrics parameters were determined in T2DM (n=23), type 2 diabetic patients with hypertension (T2DM/HT) (n=30) and controls (n=30).
  
  
  RESULTS
  T2DM/HT presented a greater inhibition of δ-ALA-D activity, a higher reactivation index (p<0.05) and a greater depletion of plasma protein thiol groups (P-SH) when compared to T2DM. Moreover, δ-ALA-D activity was positively associated with SH groups and negatively associated with serum protein carbonyl (PC) while its reactivation index was negatively associated with SH groups and positively associated with PC.
  
  
  CONCLUSIONS
  These results point out that there is a possible interference of hypertension on the mechanism of the δ-ALA-D activity suggesting that this condition aggravated the oxidative stress of diabetes mellitus.
authors.affiliation: Postgraduate Program on Pharmacology, Department of Clinical and Toxicology Analysis, Center of Healthy Sciences, Federal University of Santa Maria (UFSM), 97105-900 - Santa Maria, RS, Brazil.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Bonfanti
authors.forename: Ronise B
authors.initials: RB
authors.lastname: Ceolin
authors.forename: Karine S
authors.initials: KS
authors.lastname: De Bona
authors.forename: Leidiane
authors.initials: L
authors.lastname: de Lucca
authors.forename: Maria Beatriz
authors.initials: MB
authors.lastname: Moretto
authors.forename: Thissiane de Lima
authors.initials: Tde L
authors.lastname: Gonçalves
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D015897:Comorbidity; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000453:epidemiology* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D050050:Protein Carbonylation; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D015415:Biomarkers; D013438:Sulfhydryl Compounds; D000623:Porphobilinogen Synthase