title: In-vitro culture studies for callus and root generation of boerhaavia diffusa linn.
abstract: Leaf and stem explants excised from young plant of B.diffusa  were cultured on Murashige and Skoog (MS) medium containing agar (0.8%), sucrose (2.5%) and varied concentration of Indole butyric acid (IBA), Napthyl acetic acid (NAA), 2,4 - dichloro phenoxy acetic acid (2,4-D) and Picrolam. Leaf explants has given better response for both rooting and callus formation. IBA in a concentration of 5μM has shown maximum regeneration (69.7%) with induction period of 7 days. The developed roots were similar to that of naturally grown roots with little anatomical changes. For callus formation 20μM 2, 4-D has given maximum amount and percentage response 979.5% with an induction period of 8 days. Picrolam (10μM) has shown 36.6% response and the average weight of callus was less as compared with 2,4-D. The callus obtained was friable and opaque in nature.
authors.affiliation: B.R. Nahata College of Pharmacy, Mandsaur-458 001, M.P., India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Jain
authors.forename: D
authors.initials: D
authors.lastname: Singh
authors.forename: Swarnalata
authors.initials: S
authors.lastname: Saraf
authors.forename: S
authors.initials: S
authors.lastname: Saraf