title: An efficient protocol for isolating melanised chaetothyrialean anamorphic fungi associated with plant-ants.
abstract: Because of their ecological characteristics, slow growth rates and the presence of contaminants, Chaetothyriales fungi associated with structures built by tropical plant-ants can be difficult to isolate with standard procedures. Here, we describe an easy-to-use protocol for obtaining pure cultures by using cotton as a first substrate. We have further found by means of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the hyphae.
authors.affiliation: Université de Toulouse, UPS, UMR5174 EDB Laboratoire Evolution et Diversité Biologique, Toulouse, France. maruigon@upvnet.upv.es
authors.forename: Mario X
authors.initials: MX
authors.lastname: Ruiz-González
authors.forename: Jérémie
authors.initials: J
authors.lastname: Lauth
authors.forename: Céline
authors.initials: C
authors.lastname: Leroy
authors.forename: Alain
authors.initials: A
authors.lastname: Jauneau
authors.forename: Hervé
authors.initials: H
authors.lastname: Gryta
authors.forename: Patricia
authors.initials: P
authors.lastname: Jargeat
authors.forename: Alain
authors.initials: A
authors.lastname: Dejean
authors.forename: Jérôme
authors.initials: J
authors.lastname: Orivel
mesh_terms: D000818:Animals; D001000:Ants / Q000382:microbiology*; D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000737:chemistry; D032561:Cotton Fiber; D005456:Fluorescent Dyes / Q000737:chemistry; D005658:Fungi / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D025301:Hyphae / Q000166:cytology / Q000254:growth & development; D010860:Pigments, Biological
chemical_list: D005456:Fluorescent Dyes; D010860:Pigments, Biological