title: Human ES- and iPS-derived myogenic progenitors restore DYSTROPHIN and improve contractility upon transplantation in dystrophic mice.
abstract: A major obstacle in the application of cell-based therapies for the treatment of neuromuscular disorders is obtaining the appropriate number of stem/progenitor cells to produce effective engraftment. The use of embryonic stem (ES) or induced pluripotent stem (iPS) cells could overcome this hurdle. However, to date, derivation of engraftable skeletal muscle precursors that can restore muscle function from human pluripotent cells has not been achieved. Here we applied conditional expression of PAX7 in human ES/iPS cells to successfully derive large quantities of myogenic precursors, which, upon transplantation into dystrophic muscle, are able to engraft efficiently, producing abundant human-derived DYSTROPHIN-positive myofibers that exhibit superior strength. Importantly, transplanted cells also seed the muscle satellite cell compartment, and engraftment is present over 11 months posttransplant. This study provides the proof of principle for the derivation of functional skeletal myogenic progenitors from human ES/iPS cells and highlights their potential for future therapeutic application in muscular dystrophies.
authors.affiliation: Department of Medicine, Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Radbod
authors.initials: R
authors.lastname: Darabi
authors.forename: Robert W
authors.initials: RW
authors.lastname: Arpke
authors.forename: Stefan
authors.initials: S
authors.lastname: Irion
authors.forename: John T
authors.initials: JT
authors.lastname: Dimos
authors.forename: Marica
authors.initials: M
authors.lastname: Grskovic
authors.forename: Michael
authors.initials: M
authors.lastname: Kyba
authors.forename: Rita C R
authors.initials: RC
authors.lastname: Perlingeiro
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D016189:Dystrophin / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000502:physiology*; D005240:Feasibility Studies; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D051379:Mice; D018101:Mice, Inbred mdx; D009119:Muscle Contraction; D009136:Muscular Dystrophies / Q000628:therapy*; D051763:PAX7 Transcription Factor / Q000235:genetics / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology*; D033581:Stem Cell Transplantation; D014183:Transplantation, Heterologous
chemical_list: D016189:Dystrophin; D051763:PAX7 Transcription Factor; C497321:PAX7 protein, human