title: Endothelial membrane reorganization during leukocyte extravasation.
abstract: Leukocyte trafficking from the bloodstream to inflamed tissues across the endothelial barrier is an essential response in innate immunity. Leukocyte adhesion, locomotion, and diapedesis induce signaling in endothelial cells and this is accompanied by a profound reorganization of the endothelial cell surfaces that is only starting to be unveiled. Here we review the current knowledge on the leukocyte-mediated alterations of endothelial membrane dynamics and their role in promoting leukocyte extravasation. The formation of protein- and lipid-mediated cell adhesion nanodomains at the endothelial apical surface, the extension of micrometric apical membrane docking structures, which are derived from microvilli and embrace adhered leukocytes, as well as the vesicle-trafficking pathways that are required for efficient leukocyte diapedesis, are discussed. The coordination between these different endothelial membrane-remodeling events probably provides the road map for transmigrating leukocytes to find exit points in the vessel wall, in a context of severe mechanical and inflammatory stress. A better understanding of how vascular endothelial cells respond to immune cell adhesion should enable new therapeutic strategies to be developed that can abrogate uncontrolled leukocyte extravasation in inflammatory diseases.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, CSIC-Universidad Autónoma de Madrid, C/Nicolás Cabrera 1, Cantoblanco, 28049, Madrid, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Reglero-Real
authors.forename: Beatriz
authors.initials: B
authors.lastname: Marcos-Ramiro
authors.forename: Jaime
authors.initials: J
authors.lastname: Millán
mesh_terms: D000199:Actins / Q000502:physiology; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D021941:Caveolae / Q000378:metabolism; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007962:Leukocytes / Q000502:physiology* / Q000648:ultrastructure; D021962:Membrane Microdomains / Q000378:metabolism; D008871:Microvilli; D059470:Tetraspanins / Q000378:metabolism; D057705:Transendothelial and Transepithelial Migration / Q000502:physiology*; D014746:Vimentin / Q000502:physiology
chemical_list: D000199:Actins; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; C065289:ICAM2 protein, human; D059470:Tetraspanins; D014746:Vimentin; D018799:Intercellular Adhesion Molecule-1