title: Effects of PPAR-delta agonist and zinc on vaginal smooth muscle cells collagen and tropoelastin production.
abstract: OBJECTIVE
  The objective was to measure the effects of peroxisome proliferator-activated receptor delta (PPAR-delta) agonist and zinc sulfate (ZS) on vaginal smooth muscle cell (SMC) proliferation, tropoelastin production, and collagen production.
  
  
  METHODS
  SMC cultures were performed from vaginal wall biopsies and were incubated with ZS and PPAR-delta agonist GW501516 (GW). Cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Supernatants and cell lysates were collected. Tropoelastin production was measured by Fastin Elastin Assay and collagen was measured with Sircol Collagen Assay.
  
  
  RESULTS
  SMC proliferation was similar with 20 μM ZS or 10 nM GW compared to control. Tropoelastin production was significantly increased by 20 μM ZS and by 10 nM GW. Cell culture surface deposited elastin production was significantly increased by 20 μM ZS and by the combination of 20 μM ZS with 10 nM GW, and collagen production was significantly increased by 10 nM GW and by the combination of 20 μM ZS with 10 nM GW.
  
  
  CONCLUSIONS
  PPAR-delta agonist and ZS increases vaginal SMC tropoelastin and collagen production.
authors.affiliation: Department of Obstetrics and Gynecology, Division of Female Pelvic Medicine and Reconstructive Surgery, University of Miami, Miller School of Medicine, PO Box 016960, Miami, FL 33010, USA. ptakacs@med.miami.edu
authors.forename: Peter
authors.initials: P
authors.lastname: Takacs
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
authors.forename: Keith
authors.initials: K
authors.lastname: Candiotti
authors.forename: Sindy
authors.initials: S
authors.lastname: Jaramillo
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Medina
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism*; D047494:PPAR delta / Q000494:pharmacology*; D014333:Tropoelastin / Q000378:metabolism*; D014621:Vagina / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019287:Zinc Sulfate / Q000494:pharmacology*
chemical_list: D047494:PPAR delta; D014333:Tropoelastin; D019287:Zinc Sulfate; D003094:Collagen