title: [Reversing effects of curcumin on multi-drug resistance of Bel7402/5-fu cell line].
abstract: OBJECTIVE
  To investigate the reversing effects of curcumin on hepatocellular carcinoma drug resistance Bel7402/5-Fu cell line.
  
  
  METHODS
  Through the exposure to gradual increased concentrations of 5-fluorouracil (5-Fu), the cell line Bel7402 was induced to establish a multi-drug resistant sub-cell line Bel7402/5-Fu. The sensitivity to 6 chemotherapeutics of Bel7402 and Bel7402/5-Fu were detected using methyl thiazolyl tetrazolium (MTT) assay. The 50% inhibitory concentration (IC50) and resistant index (RI) were calculated. The differences of the inhibition ratio of Bel7402/5-Fu by curcumin, 5-Fu, curcumin combined with 5-Fu were detected using MTT assay. The effects of curcumin, 5-Fu, curcumin combined with 5-Fu on the Bel7402/5-Fu apoptosis were detected using flow cytometry.
  
  
  RESULTS
  The Bel7402/5-Fu cell line showed multi-drug resistance (MDR) to various chemotherapeutics, with the highest RI shown of 5-Fu (being 109.55 +/- 14.30 times). The inhibition ratio of 5, 10, and 20 microg/mL curcumin combined with 5-Fu (50% IC50) was respectively 21.47% +/- 1.49%, 27.10% +/- 2.32%, and 59.37% +/- 2.45%. The Bel7402/5-Fu apoptosis ratio of 5, 10, and 20 microg/mL curcumin combined with 5-Fu (50% IC50) was 30.92% +/- 2.10%, 44.87% +/- 2.24%, and 50.36% +/- 2.58%, respectively, which was obviously higher than that of the curcumin group and the 5-Fu group. Besides, the apoptosis rate increased along with increased curcumin concentration in the range of 0 -20 microg/mL.
  
  
  CONCLUSIONS
  Curcumin could induce the apoptosis of Bel7402/5-Fu. Meanwhile, it showed favorable reversing effects on MDR.
authors.affiliation: Department of Digestive Diseases, Affiliated Liyuan Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan (430077). caoshiqiong1965@126.com
authors.forename: Shi-Qiong
authors.initials: SQ
authors.lastname: Cao
authors.forename: Tai-Yong
authors.initials: TY
authors.lastname: Yin
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Yang
mesh_terms: D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans
chemical_list: D003474:Curcumin; D005472:Fluorouracil