title: Synthesis of PECAM-1-specific 64Cu PET imaging agent: evaluation of myocardial infarction caused by ischemia-reperfusion injury in mouse.
abstract: A PECAM-1 specific PET imaging agent, PECAM-1-Ab-DOTA-(64)Cu, was synthesized by conjugating the anti-mouse PECAM-1 antibody with 2,2',2",2"'-(1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrayl)tetraacetic acid (DOTA) and subsequent labeling with (64)Cu. Positron Emission Tomography (PET) was successfully performed in a mouse model of myocardial infarction (MI) induced by an ischemia/reperfusion (I/R) injury, indicating the elevated expression of PECAM-1.
authors.affiliation: Department of Radiology, The University of Virginia, 480 Ray C. Hunt Dr., Snyder Bldg, Charlottesville, VA 22908, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zequan
authors.initials: Z
authors.lastname: Yang
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Xu
authors.forename: Bijoy
authors.initials: B
authors.lastname: Kundu
authors.forename: Mahendra D
authors.initials: MD
authors.lastname: Chordia
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Pan
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003301:Copper Radioisotopes; D005682:Gadolinium / Q000737:chemistry; D015870:Gene Expression; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry; D018796:Immunoconjugates / Q000737:chemistry* / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000209:etiology / Q000473:pathology*; D015428:Myocardial Reperfusion Injury / Q000150:complications / Q000000981:diagnostic imaging / Q000473:pathology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology; D049268:Positron-Emission Tomography / Q000379:methods*; D011863:Radioimmunoassay; D019275:Radiopharmaceuticals / Q000737:chemistry* / Q000493:pharmacokinetics; D013194:Staining and Labeling / Q000379:methods; D014018:Tissue Distribution
chemical_list: D000906:Antibodies; D003301:Copper Radioisotopes; D006573:Heterocyclic Compounds, 1-Ring; D018796:Immunoconjugates; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019275:Radiopharmaceuticals; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D005682:Gadolinium