title: Application of a full-genome microarray-based assay for the study of genetic variability of West Nile virus.
abstract: Viral adaptation through fixation of spontaneous mutations is an important factor potentially associated with reoccurrence of West Nile virus (WNV) outbreaks in the New World. The emergence of new genetic variants of WNV represents an important public health issue because it may affect the sensitivity of WNV screening and diagnostic assays, as well as the development and efficacy of WNV vaccines and anti-viral drugs. A microarray assay was developed and optimized to enable simple monitoring of WNV genetic variability and rapid detection of any nucleotide mutations within the entire viral genome. The assay was validated using 11 WNV isolates from the 2007 and 2009 U.S. epidemics. The developed microarray system can potentially serve as a high throughput, rapid, and effective approach for the detection of circulating WNV genetic variants.
authors.affiliation: Laboratory of Emerging Pathogens, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, Rockville, MD 20852, USA.
authors.forename: Andriyan
authors.initials: A
authors.lastname: Grinev
authors.forename: Zhong
authors.initials: Z
authors.lastname: Lu
authors.forename: Vladimir
authors.initials: V
authors.lastname: Chizhikov
authors.forename: Maria
authors.initials: M
authors.lastname: Rios
mesh_terms: D004252:DNA Mutational Analysis; D015342:DNA Probes / Q000235:genetics; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D025202:Molecular Diagnostic Techniques; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D014901:West Nile Fever / Q000175:diagnosis / Q000821:virology*; D014902:West Nile virus / Q000235:genetics*
chemical_list: D015342:DNA Probes