title: Penetration of resveratrol into bovine aortic endothelial cells (BAEC): a possible passive diffusion.
abstract: Several studies have demonstrated that, in a context of oxidative stress, resveratrol, a polyphenol found in wine, could act as a protective agent on endothelial cells by various mechanisms but without showing that it could penetrate inside the cell. The aim of this study was to detect for the first time resveratrol inside bovine endothelial aortic cells and to determine which kind of transport mechanism was involved. Intracellular and membrane concentrations of resveratrol have been measured by high performance liquid chromatography after incubation of several concentrations of resveratrol with endothelial cells for 24h. Concentrations of resveratrol in the culture media have been determined by UV spectrophotometry and experiments of transport mechanisms have been performed. Our results showed that, for the concentrations tested (1, 5, 10 and 50 μM), resveratrol was detected inside the cells and suggested that it was able to penetrate into the cells through a passive diffusion mechanism.
authors.affiliation: EA 4466 Stress cellulaire : physiopathologie, stratégies nutritionnelles et thérapeutiques innovantes, UFR des sciences pharmaceutiques et biologiques, université Paris Descartes, Sorbonne Paris Cité, 4, avenue de l'Observatoire, 75006 Paris, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Frombaum
authors.forename: Solenn
authors.initials: S
authors.lastname: Le Clanche
authors.forename: Patrice
authors.initials: P
authors.lastname: Thérond
authors.forename: Esther
authors.initials: E
authors.lastname: Nubret
authors.forename: Dominique
authors.initials: D
authors.lastname: Bonnefont-Rousselot
authors.forename: Didier
authors.initials: D
authors.lastname: Borderie
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000633:toxicity; D001011:Aorta / Q000166:cytology / Q000378:metabolism; D001693:Biological Transport, Active / Q000502:physiology; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis; D003600:Cytosol / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D000077185:Resveratrol; D013056:Spectrophotometry, Ultraviolet; D013267:Stilbenes / Q000378:metabolism* / Q000633:toxicity; D013696:Temperature
chemical_list: D000975:Antioxidants; D003470:Culture Media; D011506:Proteins; D013267:Stilbenes; D000077185:Resveratrol