title: PpPIF-1: first isolated  full-length PIF-like element from the bamboo Phyllostachys pubescens.
abstract: PIF-like  elements are the first-described members of a recently discovered and  widespread superfamily of DNA transposons, named PIF/Harbinger. Complete  and partial PIF-like elements have been isolated from hundreds of plant  species. Previously, we identified 139 partial PIF-like transposases in  the Bambusoideae, of which three were from the bamboo species Phyllostachys  pubescens. Here we report identification and isolation of the first  full-length PIF-like element (PpPIF-1) from P. pubescens;  identification was made by chromosome walking, based on a modified magnetic  enrichment procedure that allows efficient cloning of flanking sequences up to  3 kb in length. PpPIF-1 is 5953 bp in length, with 20-bp imperfect  inverted terminal repeats and 3-bp target site duplications. This element  contains two open reading frames, one encoding a putative transposase,  including the complete DDE-domain typical of PIF/Harbinger elements from  plants, and the other encoding a DNA-binding protein. There are seven  termination codons and two frameshift mutations in the open reading frames,  probably due to vertical inactivation.
authors.affiliation: The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, LinAn, Zhejiang Province, PR China.
authors.forename: M B
authors.initials: MB
authors.lastname: Zhou
authors.forename: X M
authors.initials: XM
authors.lastname: Liu
authors.forename: D Q
authors.initials: DQ
authors.lastname: Tang
mesh_terms: D000595:Amino Acid Sequence; D031723:Bambusa / Q000235:genetics*; D001483:Base Sequence; D016386:Chromosome Walking; D017931:DNA Primers; D016368:Frameshift Mutation; D017343:Genes, Plant; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D019895:Transposases / Q000737:chemistry / Q000235:genetics
chemical_list: D017931:DNA Primers; D019895:Transposases