title: trans Arachidonic acid isomers inhibit NADPH-oxidase activity by direct interaction with enzyme components.
abstract: NADPH-oxidase is an enzyme that represents, when activated, the major source of non-mitochondrial reactive oxygen species. In phagocytes, this production is an indispensable event for the destruction of engulfed pathogens. The functional NADPH-oxidase complex consists of a catalytic membrane flavocytochrome b (Cytb(558)) and four cytosolic proteins p47(phox), p67(phox), Rac and p40(phox). The NADPH-oxidase activity is finely regulated spatially and temporally by cellular signaling events that trigger the translocation of the cytosolic subunits to its membrane partner involving post-translational modifications and activation by second messengers such as arachidonic acid (AA). Arachidonic acid in its natural cis-poly unsaturated form (C20:4) has been described to be an efficient activator of the enzyme in vivo and in vitro. In this work, we examined in a cell-free system whether a change of the natural cis geometry to the trans configuration, which could occur either by diet or be produced by the action of free radicals, may have consequences on the functioning of NADPH-oxidase. We showed the inability of mono-trans AA isomers to activate the NADPH-oxidase complex and demonstrated the inhibitory effect on the cis-AA-induced NADPH oxidase activation. The inhibition is mediated by a direct effect of the mono-trans AA which targets both the membrane fraction containing the cytb(558) and the cytosolic p67(phox). Our results suggest that the loss of the natural geometric feature (cis-AA) induces substantial structural modifications of p67(phox) that prevent its translocation to the complex.
authors.affiliation: UMR8000, CNRS, 91405 Orsay, France.
authors.forename: H
authors.initials: H
authors.lastname: Souabni
authors.forename: V
authors.initials: V
authors.lastname: Thoma
authors.forename: T
authors.initials: T
authors.lastname: Bizouarn
authors.forename: C
authors.initials: C
authors.lastname: Chatgilialoglu
authors.forename: A
authors.initials: A
authors.lastname: Siafaka-Kapadai
authors.forename: L
authors.initials: L
authors.lastname: Baciou
authors.forename: C
authors.initials: C
authors.lastname: Ferreri
authors.forename: C
authors.initials: C
authors.lastname: Houée-Levin
authors.forename: M A
authors.initials: MA
authors.lastname: Ostuni
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000737:chemistry*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D003573:Cytochrome b Group / Q000737:chemistry; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000737:chemistry; D008954:Models, Biological; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors* / Q000737:chemistry; D009504:Neutrophils / Q000378:metabolism; D010586:Phagocytes / Q000201:enzymology; D010750:Phosphoproteins / Q000737:chemistry / Q000502:physiology*; D010843:Pichia / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D013997:Time Factors; D014364:Tryptophan / Q000737:chemistry
chemical_list: D003573:Cytochrome b Group; D005227:Fatty Acids; D010750:Phosphoproteins; D011994:Recombinant Proteins; C085072:neutrophil cytosol factor 40K; C079789:neutrophil cytosol factor 67K; D016718:Arachidonic Acid; D014364:Tryptophan; C036888:cytochrome b558; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1