title: RF-Cloning.org: an online tool for the design of restriction-free cloning projects.
abstract: Restriction-free cloning (RF-cloning) is a PCR-based technology that expands on the QuikChange™ mutagenesis process originally popularized by Stratagene in the mid-1990s, and allows the insertion of essentially any sequence into any plasmid at any location. While RF-cloning is a powerful tool for the design of custom plasmids when restriction sites are not conveniently situated, manually designing the requisite primers can be tedious and error prone. We present here a web-service that automates the primer design process, along with a user interface that includes a number of useful tools for managing both the input sequences and the resulting outputs. RF-Cloning is free and open to all users, and can be accessed at http://www.rf-cloning.org.
authors.affiliation: Department of Cellular and Physiological Science, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada. biologyguy@gmail.com
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Bond
authors.forename: Christian C
authors.initials: CC
authors.lastname: Naus
mesh_terms: D000465:Algorithms; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000737:chemistry*; D020407:Internet; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012984:Software
chemical_list: D017931:DNA Primers