title: Proliferation and differentiation of neural stem cells irradiated with X-rays in logarithmic growth phase.
abstract: Exposure of the fetal brain to ionizing radiation causes congenital brain abnormalities. Normal brain formation requires regionally and temporally appropriate proliferation and differentiation of neural stem cells (NSCs) into neurons and glia. Here, we investigated the effects of X-irradiation on proliferating homogenous NSCs prepared from mouse ES cells. Cells irradiated with X-rays at a dose of 1Gy maintained the capabilities for proliferation and differentiation but stopped proliferation temporarily. In contrast, the cells ceased proliferation following irradiation at a dose of >5Gy. These results suggest that irradiation of the fetal brain at relatively low doses may cause congenital brain abnormalities as with relatively high doses.
authors.affiliation: Laboratory of Regenerative Neurosciences, Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Tokyo, Japan.
authors.forename: Mayu
authors.initials: M
authors.lastname: Isono
authors.forename: Masahiro
authors.initials: M
authors.lastname: Otsu
authors.forename: Teruaki
authors.initials: T
authors.lastname: Konishi
authors.forename: Kana
authors.initials: K
authors.lastname: Matsubara
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tanabe
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakayama
authors.forename: Nobuo
authors.initials: N
authors.lastname: Inoue
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology / Q000528:radiation effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects*; D005260:Female; D051379:Mice; D009421:Nervous System Malformations / Q000209:etiology / Q000473:pathology; D058953:Neural Stem Cells / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects*; D055495:Neurogenesis / Q000502:physiology / Q000528:radiation effects; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000528:radiation effects*; D011297:Prenatal Exposure Delayed Effects / Q000473:pathology* / Q000503:physiopathology; D011833:Radiation Injuries, Experimental / Q000473:pathology* / Q000503:physiopathology; D014965:X-Rays / Q000009:adverse effects