title: Molecular identification of human hookworm infections in economically disadvantaged communities in Peninsular Malaysia.
abstract: Species identification of human hookworm infections among eight communities in rural areas of Peninsular Malaysia was determined during 2009-2011. Fecal samples were examined by microscopy and subsequently, the internal transcribed spacer 2 and 28S ribosomal RNA region of Necator americanus and Ancylostoma spp. were sequenced. Overall, 9.1% (58 of 634) were identified positive by microscopy for hookworm infection, and 47 (81.0%) of 58 were successfully amplified and sequenced. Sequence comparison found that N. americanus (87.2%) was the most predominant hookworm identified, followed by Ancylostoma ceylanicum (23.4%). No A. duodenale infection was detected in this study. Detection of A. ceylanicum in humans highlighted the zoonotic transmission among humans living near dogs. Thus, implementation of effective control measures for hookworm infections in future should seriously consider this zoonotic implication.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur Malaysia. skyromano@gmail.com
authors.forename: Romano
authors.initials: R
authors.lastname: Ngui
authors.forename: Lee Soo
authors.initials: LS
authors.lastname: Ching
authors.forename: Tan Tiong
authors.initials: TT
authors.lastname: Kai
authors.forename: Muhammad Aidil
authors.initials: MA
authors.lastname: Roslan
authors.forename: Yvonne A L
authors.initials: YA
authors.lastname: Lim
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000722:Ancylostoma / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D000818:Animals; D002648:Child; D002675:Child, Preschool; D018509:DNA, Helminth / Q000235:genetics* / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005260:Female; D006725:Hookworm Infections / Q000175:diagnosis* / Q000453:epidemiology* / Q000469:parasitology* / Q000662:veterinary; D054884:Host-Pathogen Interactions; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D017221:Necator americanus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D012959:Socioeconomic Factors; D055815:Young Adult
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D012339:RNA, Ribosomal, 28S