title: Sequence variants in the  bovine PRDM16 gene associated with body weight in Chinese cattle breeds.
abstract: As a  zinc-finger protein, PR domain containing 16 (PRDM16) controls brown fat  determination by stimulating brown fat cell production while suppressing the  expression of genes for production of white fat cells; mutations in this domain  are associated with myelodysplastic syndrome and leukemogenesis. In our study,  polymorphisms in exons 2, 3, 4, 5, 7, 8, and 9 of the PRDM16 gene were  detected by PCR-SSCP, DNA sequencing and CRS-PCR-RFLP methods in 1031 cattle of  the Chinese breeds: Jiaxian, Nanyang, Qinchuan, and Chinese Holstein. Three  mutations (NC_007314.3: g.577 G>T, 614 T>C, 212237 T>C) were detected.  Animals with the homozygote genotype had lower body weight and average daily  gain than those with the other genotypes. PRDM16 gene-specific SNPs may  be useful markers for growth traits for marker-assisted selection programs.
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Tian
authors.forename: Y-Z
authors.initials: YZ
authors.lastname: Huang
authors.forename: X-S
authors.initials: XS
authors.lastname: Lai
authors.forename: X-Y
authors.initials: XY
authors.lastname: Lan
authors.forename: J-Q
authors.initials: JQ
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Chen
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D002681:China; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D009154:Mutation; D019655:Quantitative Trait, Heritable; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers
chemical_list: D014157:Transcription Factors