title: Piscidin-1, an antimicrobial peptide from fish (hybrid striped bass morone saxatilis x M. chrysops), induces apoptotic and necrotic activity in HT1080 cells.
abstract: Piscidin-1, a 22-residue cationic peptide isolated from mast cells of a hybrid striped bass, has potent antimicrobial activities against both gram-positive and -negative bacteria. To date, there is no report of its antitumor activity on any tumor cell lines. In this study, we examined the antitumor activity of a synthetic piscidin-1 peptide against several human cancer cell lines using an MTS assay and soft-agar colony-formation assay. We found that a low dose of piscidin induces both apoptosis and necrosis in HT1080 cells, as shown by annexin-V/propidium iodide and acridine orange/ethidium bromide staining, and triggers a necrotic cell death pathway in a short period with high-dose treatment. The destruction of cell membranes by piscidin-1 was demonstrated by transmission electron microscopy. Furthermore, piscidin-1 also inhibits the migration of HT1080 cells in a dose-dependent manner. This study provides the first evidence of the anticancer activity of the antimicrobial peptide, piscidin-1, with potential implications for the treatment of cancer.
authors.affiliation: Graduate Institute of Engineering Technology-Doctoral, National Taipei University of Technology, 1 Chung-Hsiao E. Rd., Sec. 3, Taipei 10608, Taiwan.
authors.forename: Heng-Ju
authors.initials: HJ
authors.lastname: Lin
authors.forename: Tsui-Chin
authors.initials: TC
authors.lastname: Huang
authors.forename: Sasikala
authors.initials: S
authors.lastname: Muthusamy
authors.forename: Jheng-Fong
authors.initials: JF
authors.lastname: Lee
authors.forename: Yeh-Fang
authors.initials: YF
authors.lastname: Duann
authors.forename: Cheng-Hui
authors.initials: CH
authors.lastname: Lin
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000378:metabolism* / Q000494:pharmacology*; D001492:Bass / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; D029941:Fish Proteins; C449802:moronecidin protein, Morone saxatilis