title: [Dynamic changes between osteopontin and collagen I expression in viral myocarditis mice].
abstract: OBJECTIVE
  To study the mechanism of osteopontin (OPN) in viral myocarditis by observing the expression of OPN and collagen I (Col I) in mice myocardium.
  
  
  METHODS
  The viral myocarditis models were achieved by infection with myocarditic coxsackievirus B3 (CVB3). The myocardium of mice was stained by HE and Masson staining, and the pathological scores and the collagen volume fraction (CVF )of myocardium were tabulated. The expression of Col I mRNA was measured by RT-PCR. The expression of OPN was detected by RT-PCR and ELISA.
  
  
  RESULTS
  The histopathological examination revealed a prevalence of myocardial cell necrosis and obvious inflammation changes at the 7th day post-infection. Subsequently the inflammatory lesions were gradually absorbed. At the 28th day, the inflammatory cells had almost disappeared and obvious fibrosis occurred. The pathological scores and the expression of OPN mRNA were higher than those of the control group (P<0.05), and reached the highest level at the 7th day (P<0.05). From the 14th day, these parameters decreased,reflected also in the ELISA results. At the 7th day and the 14th day, the Col I expression was similar to that of control. Col I expression at the 21th and 28th days was higher than those of the control (P<0.05), and correlated positively to the CVF results.
  
  
  CONCLUSIONS
  The OPN mRNA expression increased in acute stage of VMC, and higher than that of the control group when in recovery stage, suggesting that OPN might be related to the inflammatory response in acute stage of, and promote the collagen synthesis of recovery stage.
authors.affiliation: Emergency Center of Hunan Children's Hospital, Changsha 410000, China. ccyzdh@126.com
authors.forename: Zili
authors.initials: Z
authors.lastname: Cai
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Linfeng
authors.initials: L
authors.lastname: Huang
authors.forename: Liang
authors.initials: L
authors.lastname: Cheng
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Chunyuan
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism*; D003384:Coxsackievirus Infections / Q000378:metabolism*; D029822:Enterovirus B, Human / Q000302:isolation & purification; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000378:metabolism / Q000821:virology*; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D012333:RNA, Messenger; C506896:Spp1 protein, mouse; D053495:Osteopontin