title: A novel BTK-like protein involved in immune response in Lethenteron japonicum.
abstract: Bruton's tyrosine kinase (BTK) plays an important role in the development, differentiation and signal transduction of B-lymphocyte. Lymphocyte-like cells and unique variable lymphocyte receptors have been found in the Japanese lamprey Lethenteron japonicum, an agnatha known to be lacking T or B lymphocyte, and the oldest agantha in existence. In this study a BTK-like gene from L. japonicum was identified and characterized. It contained a 1992-bp open reading frame (ORF), a 157-bp 5'-untranslated region (UTR) and a 567-bp 3'-UTR. Phylogenetic analysis indicated it had high sequence homology with BTKs from other vertebrates. Real-time PCR analysis showed that the transcript of the L. japonicum BTK-like gene was distributed in various immune associated tissues, and the level of which was upregulated in leukocytes after the animal was stimulated with LPS. This observation was further confirmed by FACS and western blot analyses. These results indicated that the BTK-like gene identified in L. japonicum may play an important role in the immune response of lamprey.
authors.affiliation: College of Life Science, Liaoning Normal University, Dalian 116029, China.
authors.forename: Fenfang
authors.initials: F
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Liyong
authors.initials: L
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Su
authors.forename: Yinglun
authors.initials: Y
authors.lastname: Han
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000077329:Agammaglobulinaemia Tyrosine Kinase; D000595:Amino Acid Sequence; D000818:Animals; D004926:Escherichia coli; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D007113:Immunity, Innate; D007798:Lampreys / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D029941:Fish Proteins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase