title: Influence of exposure time on gene expression by human intestinal epithelial cells exposed to Lactobacillus acidophilus.
abstract: Analysis of global temporal gene expression by human intestinal cells when exposed to Lactobacillus acidophilus revealed induction of immune-related pathways and NF-κB target genes after a 1-h exposure, compared to a 4- or 8-h exposure. Additionally, an L. acidophilus derivative expressing covalently bound flagellin resulted in increased induction of il8, cxc1, and cxcl2 compared to the parent L. acidophilus.
authors.affiliation: Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: O'Flaherty
authors.forename: Todd R
authors.initials: TR
authors.lastname: Klaenhammer
mesh_terms: D018938:Caco-2 Cells; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D005408:Flagellin / Q000378:metabolism / Q000494:pharmacology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000276:immunology / Q000382:microbiology*; D007779:Lactobacillus acidophilus / Q000276:immunology*; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D019936:Probiotics / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D013997:Time Factors
chemical_list: D016328:NF-kappa B; D011994:Recombinant Proteins; D005408:Flagellin