title: N-Latex CDT results in liver diseases.
abstract: OBJECTIVE
  The aim of this study was to test whether liver diseases of alcoholic and non-alcoholic origin cause false-positive carbohydrate-deficient transferrin (CDT) results when the particle-enhanced immunonephelometry for CDT assays is used and to assess the effect of liver disease severity on N-Latex CDT results.
  
  
  METHODS
  Blood was sampled from 245 newly admitted patients suffering from liver diseases: alcoholic and non-alcoholic cirrhosis (AC), chronic viral (B and C) and non-viral hepatitis, toxic and autoimmune hepatitis (AIH), hepatocellular carcinoma and primary biliary cirrhosis (PBC). CDT was determined by particle-enhanced imunononephelometry using the N-Latex CDT test.
  
  
  RESULTS
  There were significant differences in %CDT levels between liver diseases of various etiologies. The %CDT level in AC was higher than that in chronic hepatitis (non-viral and viral C). In turn, the %CDT level in chronic hepatitis C was lower than that in toxic hepatitis. The frequency of false-positive %CDT results in liver diseases of non-alcoholic origin was 13/146, and was highest in AIH (4/14). There were no CDT-positive results in PBC and chronic hepatitis B. The frequency of CDT-positive results in alcoholic liver diseases was 24/59 in cirrhosis and 10/34 in hepatitis. Serum levels of %CDT in cirrhotic patients are correlated with the severity of the disease assessed by the Child-Pugh score.
  
  
  CONCLUSIONS
  We concluded that the liver diseases affect the relative but not absolute values of CDT when using the assay with the monoclonal antibodies directed against CDT. The CDT results from N-Latex CDT test reflect the severity of liver dysfunction.
authors.affiliation: Department of Biochemical Diagnostics, Medical University, Waszyngtona 15A, 15-269 Bialystok, Poland. chrostek@umwb.edu.pl
authors.forename: Lech
authors.initials: L
authors.lastname: Chrostek
authors.forename: Bogdan
authors.initials: B
authors.lastname: Cylwik
authors.forename: Ewa
authors.initials: E
authors.lastname: Gruszewska
authors.forename: Anatol
authors.initials: A
authors.lastname: Panasiuk
authors.forename: Maciej
authors.initials: M
authors.lastname: Szmitkowski
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000437:Alcoholism / Q000150:complications / Q000175:diagnosis*; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D008107:Liver Diseases / Q000097:blood* / Q000150:complications; D008111:Liver Function Tests / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D018709:Statistics, Nonparametric; D014168:Transferrin / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D015415:Biomarkers; D014168:Transferrin; C056759:carbohydrate-deficient transferrin