title: Angustibacter peucedani sp. nov., isolated from rhizosphere soil.
abstract: A strictly aerobic, Gram-stain-positive actinobacterial strain was isolated from a rhizosphere soil of a wild plant (Peucedanum japonicum Thumb.) collected on Mara Island, Jeju, Republic of Korea. Cells of strain RS-50(T) were oxidase-negative, catalase-positive, short rods and motile by means of a polar flagellum; the colonies were orange, circular, smooth and convex. meso-Diaminopimelic acid and glucose were the diagnostic diamino acid in the cell wall and the whole-cell sugar, respectively. The major menaquinone was MK-9(H(4)). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, two unknown phospholipids and an unknown lipid. The major fatty acids were iso-C(15 : 0), iso-C(16 : 0,) anteiso-C(15 : 0) and anteiso-C(17 : 0). The DNA G+C content was 73.6 mol%. In 16S rRNA gene sequence-based phylogenetic trees, strain RS-50(T) formed a tight cluster with Angustibacter luteus (99.2 % sequence similarity); both were loosely related to the suborders Kineosporiineae and Micrococcineae. The DNA-DNA relatedness value of the isolate to A. luteus KACC 14249(T) was 22.3±0.9 %. On the basis of the results of phenotypic analyses and DNA-DNA hybridization experiments, strain RS-50(T) is considered to represent a novel species of the genus Angustibacter, for which the name Angustibacter peucedani sp. nov. is proposed. The type strain is RS-50(T) ( = KCTC 19628(T) = DSM 45329(T)). The description of the genus Angustibacter is emended.
authors.affiliation: Department of Science Education, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Soon Dong
authors.initials: SD
authors.lastname: Lee
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D019661:Apiaceae / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9