title: Saponins with neuroprotective effects from the roots of Pulsatilla cernua.
abstract: Four new oleanene-type triterpenoid saponins together with six known saponins were isolated from the roots of Pulsatilla cernua and their structures were elucidated on the basis of spectroscopic data, including 2D NMR spectra and chemical evidence. Among these one of the aglycones (gypsogenin) is reported for the first time from this genus. Some of these compounds showed significant neuroprotective effects against the cytotoxicity induced by β-amyloid(25-35) (Aβ(25-35)) on human neuroblastoma SH-SY5Y cells.
authors.affiliation: Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. burningice@126.com
authors.forename: Jian-Yu
authors.initials: JY
authors.lastname: Liu
authors.forename: Ying-Li
authors.initials: YL
authors.lastname: Guan
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Zou
authors.forename: Yi-Xia
authors.initials: YX
authors.lastname: Gong
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Hua
authors.forename: Yong-Nan
authors.initials: YN
authors.lastname: Xu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Zong-Gui
authors.initials: ZG
authors.lastname: Yu
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Fan
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002681:China; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D018696:Neuroprotective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal; D031946:Pulsatilla / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes