title: Amino acid differences in interferon-tau (IFN-τ) of Bos taurus Coreanae and Holstein.
abstract: Interferons (IFNs) are commonly grouped into type I and type II IFN. Type I IFNs are known as antiviral IFNs including IFN-α, IFN-β, and IFN-ω whereas type II IFN is referred to immune IFN and IFN-γ is only member of the type II IFN. Type I IFNs are induced by virus invading however type II IFN is produced by mitogenic or antigenic stimuli. IFN-τ was first identified in ruminant ungulates as a pregnancy recognition hormone, trophoblastin. IFN-τ constitutes a new class of type I IFN, which possesses the common features of type I IFN, such as the ability to prevent viral infection and to limit cell proliferation. In addition, IFN-τ is unique in that it is induced by pregnancy unlike other type I IFNs. We cloned Bos taurus (B. T.) Coreanae IFN-τ from peripheral blood mononuclear cells. The amino acid sequence of B. T. Coreanae IFN-τ shares only 90.3% identity with that of Holstein dairy cow. Recombinant B. T. Coreanae and Holstein IFN-τ proteins were expressed in Escherichia coli and the antiviral activity of IFN-τ proteins were examined. Both recombinant proteins were active and protected human WISH and bovine MDBK cells from the cytopathic effect of vesicular stomatitis virus. The recombinant IFN-τ protein of B. T. Coreanae and Holstein properly induced the expression of antiviral genes including 2',5'-oligoadenylate synthetase (OAS) and Mx GTPase 1 (Mx-1).
authors.affiliation: College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Dongjun
authors.initials: D
authors.lastname: Kang
authors.forename: Soyoon
authors.initials: S
authors.lastname: Ryoo
authors.forename: Byunghyun
authors.initials: B
authors.lastname: Chung
authors.forename: Joongbok
authors.initials: J
authors.lastname: Lee
authors.forename: Seungyoung
authors.initials: S
authors.lastname: Park
authors.forename: Jinsoo
authors.initials: J
authors.lastname: Han
authors.forename: Sangmin
authors.initials: S
authors.lastname: Jeong
authors.forename: Gyujin
authors.initials: G
authors.lastname: Rho
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Hong
authors.forename: Suyoung
authors.initials: S
authors.lastname: Bae
authors.forename: Taebong
authors.initials: T
authors.lastname: Kang
authors.forename: Soseob
authors.initials: S
authors.lastname: Kim
authors.forename: Soohyun
authors.initials: S
authors.lastname: Kim
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D001773:Blood Cells / Q000187:drug effects / Q000276:immunology; D002417:Cattle / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004285:Dogs; D020558:GTP Phosphohydrolases / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D011257:Pregnancy Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D000998:Antiviral Agents; D007370:Interferon Type I; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C020947:interferon tau; D015088:2',5'-Oligoadenylate Synthetase; D020558:GTP Phosphohydrolases