title: Membrane-assisted extractive butanol fermentation by Clostridium saccharoperbutylacetonicum N1-4 with 1-dodecanol as the extractant.
abstract: A polytetrafluoroethylene (PTFE) membrane was used in membrane-assisted extractive (MAE) fermentation of acetone-butanol-ethanol (ABE) by Clostridium saccharoperbutylacetonicum N1-4. The growth inhibition effects of 1-dodecanol, which has a high partition coefficient for butanol, can be prevented by employing 1-dodecanol as an extractant when using a PTFE membrane. Compared to conventional fermentation, MAE-ABE fermentation with 1-dodecanol decreased butanol inhibition and increased glucose consumption from 59.4 to 86.0 g/L, and total butanol production increased from 16.0 to 20.1g/L. The maximum butanol production rate increased from 0.817 to 0.979 g/L/h. The butanol productivity per membrane area was remarkably high with this system, i.e., 78.6g/L/h/m(2). Therefore, it is expected that this MAE fermentation system can achieve footprint downsizing.
authors.affiliation: Laboratory of Applied Microbiology, Department of Applied Biochemistry and Food Science, Faculty of Agriculture, Saga University, Honjo-cho 1, Saga 840-8502, Japan.
authors.forename: Shigemitsu
authors.initials: S
authors.lastname: Tanaka
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Tashiro
authors.forename: Genta
authors.initials: G
authors.lastname: Kobayashi
authors.forename: Toru
authors.initials: T
authors.lastname: Ikegami
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Negishi
authors.forename: Keiji
authors.initials: K
authors.lastname: Sakaki
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D000096:Acetone / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D003013:Clostridium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D007851:Dodecanol / Q000494:pharmacology* / Q000633:toxicity; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000502:physiology*; D059625:Liquid-Liquid Extraction; D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene
chemical_list: D008567:Membranes, Artificial; D000096:Acetone; D007851:Dodecanol; D000431:Ethanol; D020001:1-Butanol; D011138:Polytetrafluoroethylene