title: Effects of margarine supplemented with t10c12 and C9T11 CLA on atherosclerosis and steatosis in apoE/LDLR -/- mice.
abstract: OBJECTIVE
  The objective of this study was to evaluate functional effects of margarine supplemented with individual CLA isomers trans-10, cis-12 and cis-9, trans-11 in apoE/LDLR -/- mice.
  
  
  METHODS
  In LONG experiment (LONG), two-month old mice with no atherosclerosis were assigned to experimental groups and fed for the next 4 months. In SHORT experiment (SHORT), four-month old mice, with pre-established atherosclerosis, were assigned to experimental groups and fed for the next 2 months. The experimental diets were: AIN-93G (margarine), AIN-93G + 0.5% trans-10, cis-12 CLA (t10c12), and AIN-93G + 0.5% cis-9, trans-11 CLA (c9t11).
  
  
  RESULTS
  In both experiments (LONG and SHORT), liver weight was significantly (P<0.05) increased in mice fed t10c12 CLA. Hepatic steatosis was found in animals fed t10c12 diet and no signs of the steatosis was observed in mice fed c9t11 CLA. Dietary treatments with t10c12 CLA significantly increased total plasma cholesterol and plasma triacylglycerols. There were no isomer-specific effects of CLA isomers on area of atherosclerotic plaque in aortic root.
  
  
  CONCLUSIONS
  In conclusion, t10c12 CLA significantly increased liver weight in mice in LONG and SHORT experiments. Our results do not support the notion that CLA isomer supplementation to the margarine possess anti-atheroclerotic effect. Therefore, no isomer-specific effects of CLA on development of atherosclerosis were observed.
authors.affiliation: Department of Human Nutrition, Faculty of Food Technology, Agricultural University of Kraków, ul. Balicka 122, 30-149 Kraków, Poland. rkostogrys@ar.krakow.pl
authors.forename: R B
authors.initials: RB
authors.lastname: Kostogrys
authors.forename: M
authors.initials: M
authors.lastname: Franczyk-Żarów
authors.forename: E
authors.initials: E
authors.lastname: Maślak
authors.forename: M
authors.initials: M
authors.lastname: Gajda
authors.forename: Ł
authors.initials: Ł
authors.lastname: Mateuszuk
authors.forename: S
authors.initials: S
authors.lastname: Chłopicki
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology; D050197:Atherosclerosis / Q000097:blood* / Q000209:etiology; D002784:Cholesterol / Q000097:blood*; D004041:Dietary Fats / Q000008:administration & dosage; D019587:Dietary Supplements; D005234:Fatty Liver / Q000097:blood / Q000139:chemically induced*; D005527:Food, Fortified; D007536:Isomerism; D044243:Linoleic Acids, Conjugated / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008383:Margarine / Q000009:adverse effects*; D051379:Mice; D018345:Mice, Knockout; D009929:Organ Size; D058226:Plaque, Atherosclerotic / Q000473:pathology; D014280:Triglycerides / Q000097:blood*
chemical_list: D004041:Dietary Fats; D044243:Linoleic Acids, Conjugated; D014280:Triglycerides; D008383:Margarine; D002784:Cholesterol