title: [Effects of norcantharidin on lipopolysaccharide-induced hepatocyte injury in vitro].
abstract: OBJECTIVE
  To study the effects of norcantharidin (NCTD) on lipopolysaccharide (LPS)-induced hepatocyte injury and the expression of TNF-α and IL-6 in vitro.
  
  
  METHODS
  Hepatocytes were isolated from male Sprague-Dawley rats by collagenase perfusion. LPS at concentration of 40 mg/L was used to induce injury to the cultured cells, and NCTD (0.5, 1.0, 2.5 μg/mL) was added at the same time. After 24 h of incubation, the cell proliferation rates were detected by MTT. LDH, TNF-α and IL-6 were measured by appropriate reagent kits.NF-κB DNA binding activity was measured.
  
  
  RESULTS
  40 mg/L LPS caused a 27% growth inhibition in primary hepatocytes. LDH leakage was 20- fold higher in NCTD-treated hepatocytes than in normal ones. TNF-α and IL-6 expression significantly increased. In cells treated with NCTD at doses of 0.5, 1.0 and 2.5 μg/mL, LDH leakage, TNF-α and IL-6 expression, and NF-κB DNA binding activity were attenuated in a dose dependent manner.
  
  
  CONCLUSIONS
  NCTD protects hepatocytes from injury induced by LPS; the protection is associated with suppression of the inflammatory cytokine TNF-α and IL-6.
authors.affiliation: Department of Minimal Invasive Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Qunwei
authors.initials: Q
authors.lastname: Wang
authors.forename: Wenbin
authors.initials: W
authors.lastname: Duan
mesh_terms: D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D049109:Cell Proliferation; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D061251:Primary Cell Culture; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D004268:DNA-Binding Proteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; C069741:norcantharidin; D007770:L-Lactate Dehydrogenase