title: Analysis of deoxynucleosides in bacteriophages ϕEF24C and K and the frequency of a specific restriction site in the genomes of members of the bacteriophage subfamily Spounavirinae.
abstract: The genera SPO1-like and Twort-like viruses in the subfamily Spounavirinae of the family Myoviridae have been newly proposed, with the reorganization of the SPO1-related bacteriophages (phages). A criterion defining these viral genera is the presence/absence of DNA modifications. In this study, liquid chromatography/mass spectrometry showed that phages ϕEF24C and K of the subfamily Spounavirinae have unmodified DNA, which classifies them as Twort-like viruses. Moreover, in the subfamily Spounavirinae, DNA modification and elimination of a particular DNA sequence were suggested to be the major antirestriction strategies of the SPO1-like and Twort-like viruses, respectively.
authors.affiliation: Department of Microbiology and Infection, Kochi University, Kohasu, Oko-cho, Nankoku City, Kochi 783-8505, Japan. jumpeiu@kochi-u.ac.jp
authors.forename: Jumpei
authors.initials: J
authors.lastname: Uchiyama
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Maeda
authors.forename: Iyo
authors.initials: I
authors.lastname: Takemura
authors.forename: Keiji
authors.initials: K
authors.lastname: Gamoh
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: Masanori
authors.initials: M
authors.lastname: Daibata
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D017900:Myoviridae / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D015183:Restriction Mapping; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins