title: Solution structure of the ESCRT-I and -II supercomplex: implications for membrane budding and scission.
abstract: The ESCRT-I and ESCRT-II supercomplex induces membrane buds that invaginate into the lumen of endosomes, a process central to the lysosomal degradation of ubiquitinated membrane proteins. The solution conformation of the membrane-budding ESCRT-I-II supercomplex from yeast was refined against small-angle X-ray scattering (SAXS), single-molecule Förster resonance energy transfer (smFRET), and double electron-electron resonance (DEER) spectra. These refinements yielded an ensemble of 18 ESCRT-I-II supercomplex structures that range from compact to highly extended. The crescent shapes of the ESCRT-I-II supercomplex structures provide the basis for a detailed mechanistic model, in which ESCRT-I-II stabilizes membrane buds and coordinates cargo sorting by lining the pore of the nascent bud necks. The hybrid refinement used here is general and should be applicable to other dynamic multiprotein assmeblies.
authors.affiliation: Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Evzen
authors.initials: E
authors.lastname: Boura
authors.forename: Bartosz
authors.initials: B
authors.lastname: Różycki
authors.forename: Hoi Sung
authors.initials: HS
authors.lastname: Chung
authors.forename: Dawn Z
authors.initials: DZ
authors.lastname: Herrick
authors.forename: Bertram
authors.initials: B
authors.lastname: Canagarajah
authors.forename: David S
authors.initials: DS
authors.lastname: Cafiso
authors.forename: William A
authors.initials: WA
authors.lastname: Eaton
authors.forename: Gerhard
authors.initials: G
authors.lastname: Hummer
authors.forename: James H
authors.initials: JH
authors.lastname: Hurley
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D018360:Crystallography, X-Ray; D056827:Endosomal Sorting Complexes Required for Transport / Q000737:chemistry* / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D053838:Scattering, Small Angle; D012996:Solutions
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D012996:Solutions