title: [Transcriptional activities of tumor-specific survivin promoter and PSMA promoter and enhancer in human prostate cancer: evaluation and comparison].
abstract: OBJECTIVE
  To detect and compare the transcriptional activities of prostate-specific membrane antigen (PSMA) promoter and enhancer and survivin promoter in different human prostate cancer cell lines, and to search for some evidence for the targeting gene therapy of human prostate cancer.
  
  
  METHODS
  The fragments of the PSMA promoter and enhancer and survivin promoter were amplified by PCR and inserted into pGL3-Basic. The recombinant plasmids were transiently transfected into human prostate cancer cell lines and normal Chang liver cells, and, their transcriptional activities in various cells were determined by measuring the expression of luciferase.
  
  
  RESULTS
  The survivin promoter exhibited a higher transcriptional activity than PSMA promoter and enhancer in tumor cell lines, and the S2pro promoter showed the highest activity, reaching one third of that of the CMV promoter.
  
  
  CONCLUSIONS
  The survivin promoter is highly activated in prostate cancer cell lines and may serve as a new tool for the transcriptional targeting gene therapy of prostate cancer.
authors.affiliation: Department of Clinical Laboratory, The 97th Hospital of PLA, Xuzhou, Jiangsu 221004, China. xiaomeiluo@163.com
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Luo
authors.forename: Jia-Yun
authors.initials: JY
authors.lastname: Liu
authors.forename: Ming-Quan
authors.initials: MQ
authors.lastname: Su
authors.forename: Xiao-Ke
authors.initials: XK
authors.lastname: Hao
mesh_terms: D000954:Antigens, Surface / Q000235:genetics*; D045744:Cell Line, Tumor; D043425:Glutamate Carboxypeptidase II / Q000235:genetics*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D008297:Male; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000628:therapy; D000077022:Survivin; D024363:Transcription Initiation Site; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D000954:Antigens, Surface; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II