title: Ag@AgI, core@shell structure in agarose matrix as hybrid: synthesis, characterization, and antimicrobial activity.
abstract: A novel in situ core@shell structure consisting of nanoparticles of Ag (Ag Nps) and AgI in agarose matrix (Ag@AgI/agarose) has been synthesized as a hybrid, in order to have an efficient antibacterial agent for repetitive usage with no toxicity. The synthesized core@shell structure is very well characterized by XRD, UV-visible, photoluminescence, and TEM. A detailed antibacterial studies including repetitive cycles are carried out on Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria in saline water, both in dark and on exposure to visible light. The hybrid could be recycled for the antibacterial activity and is nontoxic toward human cervical cancer cells (HeLa cells). The water insoluble Ag@AgI in agarose matrix forms a good coating on quartz, having good mechanical strength. EPR and TEM studies are carried out on the Ag@AgI/agarose and the bacteria, respectively, to elucidate a possible mechanism for killing of the bacteria.
authors.affiliation: Solid State and Structural Chemistry Unit, Indian Institute of Science, Bangalore-560 012, India.
authors.forename: Somnath
authors.initials: S
authors.lastname: Ghosh
authors.forename: A
authors.initials: A
authors.lastname: Saraswathi
authors.forename: S S
authors.initials: SS
authors.lastname: Indi
authors.forename: S L
authors.initials: SL
authors.lastname: Hoti
authors.forename: H N
authors.initials: HN
authors.lastname: Vasan
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D017743:Equipment Reuse; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006367:HeLa Cells; D006801:Humans; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D050296:Microbial Viability / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011791:Quartz / Q000737:chemistry; D054712:Salinity; D012685:Sepharose / Q000737:chemistry*; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D013053:Spectrophotometry; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors; D014867:Water; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D014867:Water; D011791:Quartz; D012834:Silver; D012685:Sepharose