title: [Protective effects of ischemic postconditioning on non-heart-beating donor in rat lung transplantation].
abstract: OBJECTIVE
  To explore the protective effects of ischemic postconditioning on non-heart-beating donor (NHBD) in rat NHBD lung transplantation model.
  
  
  METHODS
  Forty Sprague-Dawley rats were randomized into the ischemic postconditioning group (IPO group) and the control group (C group), 10 pairs in each group in which left lung orthotopic transplantations from NHBDs were done with " two-cuff-one-stent technique". In the C group, perfusion was resumed by declamping pulmonary artery immediately after transplantation, whereas in the IPO group, 5 cycles of 1-min reperfusion and 1-min reocclusion of pulmonary artery were applied as postcontioning before full recovery of perfusion.
  
  
  RESULTS
  Compared with the C group, water content of donor lungs was lower and pathological changes were milder in the IPO group, meanwhile compliance, structure and function of donor lungs were better preserved. Furthermore, the expression of cell apoptosis and MDA content in donor lungs were lower in the IPO group, while SOD content was higher.
  
  
  CONCLUSIONS
  Ischemic postconditioning can reduce ischemic reperfusion injury of NHBD lung transplantation and preserve the structure and function of donor lungs. It can inhibit lipid peroxidation and cell apoptosis in NHBD lungs after transplantation.
authors.affiliation: Department of Cardiothoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China. drhqh240@163.com
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Hu
authors.forename: Shengxi
authors.initials: S
authors.lastname: Chen
authors.forename: Fanyan
authors.initials: F
authors.lastname: Luo
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D006323:Heart Arrest; D057775:Ischemic Postconditioning; D008168:Lung / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D016040:Lung Transplantation; D008297:Male; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D013482:Superoxide Dismutase