title: Investigation of immunogenicity of cryopreserved limbal stem cells.
abstract: OBJECTIVE
  To investigate changes in immunogenicity of cryopreserved limbal stem cells.
  
  
  METHODS
  Cryopreserved limbal stem cells, fresh primary limbal stem cells and blank controls were inoculated subcutaneously in C57BL-6 mice and the percentage of CD25 cells in limbal explants was determined by flow cytometry at day 21 post inoculation. Morphological studies were performed by light and electron microscopy of limbal explant sections.
  
  
  RESULTS
  The number of regional and systemic lymphocytes derived from cryopreserved limbal stem cells was lower than that from fresh primary limbal stem cells.
  
  
  CONCLUSIONS
  Lymphocytes derived from cryopreserved limbal stem cells showed changes in immunogenicity, but the significance is unknown. The cryopreservation and thawing methods await further study.
authors.affiliation: Department of Ophthalmology, First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Cui-Xia
authors.initials: CX
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Sun