title: Fast-mode duplex qPCR for BCR-ABL1 molecular monitoring: innovation, automation, and harmonization.
abstract: Reverse transcription quantitative polymerase chain reaction (RTqPCR)is currently the most sensitive tool available for the routine monitoring of disease level in patients undergoing treatment for BCRABL1 associated malignancies. Considerable effort has been invested at both the local and international levels to standardise the methodology and reporting criteria used to assess this critical metric. In an effort to accommodate the demands of increasing sample throughput and greater standardization, we adapted the current best-practice guidelines to encompass automation platforms and improved multiplex RT-qPCR technology.
authors.affiliation: Imperial Molecular Pathology, Imperial College Academic Health Science Centre, London, United Kingdom. g.gerrard@imperial.ac.uk
authors.forename: Gareth
authors.initials: G
authors.lastname: Gerrard
authors.forename: Katherine
authors.initials: K
authors.lastname: Mudge
authors.forename: Pierre
authors.initials: P
authors.lastname: Foskett
authors.forename: David
authors.initials: D
authors.lastname: Stevens
authors.forename: Mary
authors.initials: M
authors.lastname: Alikian
authors.forename: Helen E
authors.initials: HE
authors.lastname: White
authors.forename: Nicholas C P
authors.initials: NC
authors.lastname: Cross
authors.forename: Jane
authors.initials: J
authors.lastname: Apperley
authors.forename: Letizia
authors.initials: L
authors.lastname: Foroni
mesh_terms: D057205:Automation, Laboratory; D015415:Biomarkers; D004059:Diffusion of Innovation; D016044:Fusion Proteins, bcr-abl / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D057166:High-Throughput Screening Assays / Q000592:standards; D006801:Humans; D007700:Kinetics; D057230:Limit of Detection; D015335:Molecular Probes / Q000378:metabolism; D060885:Multiplex Polymerase Chain Reaction; D009363:Neoplasm Proteins; D016315:Proto-Oncogene Proteins c-abl / Q000097:blood / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D015335:Molecular Probes; D009363:Neoplasm Proteins; C406060:abl-bcr fusion protein, human; D016044:Fusion Proteins, bcr-abl; D016315:Proto-Oncogene Proteins c-abl