title: [Effects of smoking and tumor process on the contents of key proteins of apoptosis and activity of antioxidant enzymes in blood].
abstract: The effects of smoking on the contents of the apoptosis markers Bcl-2 and p53 proteins in blood plasma; the activity of the antioxidant (AO) enzymes Cu, Zn-superoxide dismutase (SOD), glutathione peroxidase (GP), glutathione reductase (GR), glutathione S-transferase (GST), and catalase; and the content of malondialdehyde (MDA) in erythrocytes from healthy donors and cancer patients were studied. Two groups of donors were revealed among healthy smokers: one with high SOD and GP activities and high Bcl-2 protein levels and the other with lower Bcl-2 levels compared with those found in nonsmokers. In the group of cancer patients (both smokers and nonsmokers), significantly increased p53 protein levels and increased activity of GST were found. A negative correlation between MDA and GST in the group of smoking healthy donors and a positive correlation between MDA and p53 in cancer patients were found. The results suggest a relationship between the components of enzymatic defence and lipid peroxidation and the content of apoptosis regulator proteins in healthy smokers and cancer patients.
authors.forename: E M
authors.initials: EM
authors.lastname: Mil'
authors.forename: S M
authors.initials: SM
authors.lastname: Gurevich
authors.forename: A I
authors.initials: AI
authors.lastname: Kozachenko
authors.forename: L G
authors.initials: LG
authors.lastname: Nagler
authors.forename: A A
authors.initials: AA
authors.lastname: Albantova
authors.forename: L D
authors.initials: LD
authors.lastname: Fatkullina
authors.forename: E B
authors.initials: EB
authors.lastname: Burlakova
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism*; D005980:Glutathione Reductase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation; D008175:Lung Neoplasms / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000097:blood*; D012016:Reference Values; D012907:Smoking / Q000097:blood*; D013482:Superoxide Dismutase / Q000097:blood; D016159:Tumor Suppressor Protein p53 / Q000097:blood*
chemical_list: D000975:Antioxidants; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase