title: MicroRNA-181a promotes gastric cancer by negatively regulating tumor suppressor KLF6.
abstract: MicroRNAs have emerged as crucial regulators of tumorigenesis. However, it remains unknown whether miR-181a is involved in the pathogenesis of gastric cancer. In this study, we found that miR-181a is overexpressed in human gastric cancer tissues. Ectopic expression of miR-181a mimic promoted the proliferation, colony formation, migration, and invasion and inhibited the apoptosis of SGC-7901 gastric cancer cells, whereas ectopic expression of miR-181a inhibitor inhibited the malignant phenotypes of SGC-7901 cells. Site-directed mutagenesis and luciferase reporter assay demonstrated that miR-181a repressed KLF6 expression by targeting its 3'-UTR. Western blot analysis further showed that KLF6 protein was significantly decreased or increased when miR-181a mimic or inhibitor was transfected into SGC-7901 cells, respectively. In summary, these data suggest that KLF6 gene is a direct target of miR-181a and miR-181a functions as an oncomir in gastric cancer by repressing the expression of tumor suppressor KLF6.
authors.affiliation: Guangzhou Key Laboratory of Digestive Disease, Department of Gastroenterology, First Municipal People's Hospital of Guangzhou, Guangzhou Medical University, No.1 Panfu Road, Guangzhou, 510180, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhang
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Nie
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Du
authors.forename: Jie
authors.initials: J
authors.lastname: Cao
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.forename: Yuyuang
authors.initials: Y
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C105465:KLF6 protein, human; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins