title: Full-term development of gaur-bovine interspecies somatic cell nuclear transfer embryos: effect of trichostatin A treatment.
abstract: Trichostatin A (TSA) has previously been used in somatic cell nuclear transfer (SCNT) to improve the cloning efficiency in several species, which led our team to investigate the effects of TSA on the full-term development of bovine SCNT and gaur-bovine interspecies SCNT (gaur iSCNT; gaur somatic cells as donors and bovine oocytes as recipients) embryos. Treatment with 50 nM TSA for 10 h after fusion had no positive effects on the rates of fusion, cleavage, or the development to eight-cell or morula stages in both bovine SCNT and gaur iSCNT embryos. However, TSA treatment significantly enhanced the blastocyst formation rate in bovine SCNT embryos (44 vs. 32-34% in the TSA-treated and TSA-untreated groups, respectively), but had no effects on gaur iSCNT embryos. The fresh blastocysts derived from bovine SCNT and gaur iSCNT embryos (fresh groups), as well as vitrified bovine SCNT blastocysts (vitrified group), were transferred to bovine recipients. We found that TSA treatment increased the pregnancy rates only in recipients receiving fresh bovine SCNT embryos. In recipients receiving TSA-treated bovine SCNT embryos, three cloned calves from the fresh group and twin cloned calves from the vitrified group were delivered; however, no calf was born from the TSA-untreated bovine SCNT embryos. In contrast, one gaur iSCNT calf was born from a recipient receiving blastocysts from the TSA-untreated group. In summary, TSA improved the preimplantation development and pregnancy rates of bovine SCNT embryos, but did not have any beneficial effect on gaur iSCNT embryos. However, one gaur iSCNT calf reached full-term development.
authors.affiliation: Embryo Technology and Stem Cell Research Center and School of Biotechnology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
authors.forename: Kanokwan
authors.initials: K
authors.lastname: Srirattana
authors.forename: Sumeth
authors.initials: S
authors.lastname: Imsoonthornruksa
authors.forename: Chuti
authors.initials: C
authors.lastname: Laowtammathron
authors.forename: Anawat
authors.initials: A
authors.lastname: Sangmalee
authors.forename: Wanchai
authors.initials: W
authors.lastname: Tunwattana
authors.forename: Thamnoon
authors.initials: T
authors.lastname: Thongprapai
authors.forename: Chockchai
authors.initials: C
authors.lastname: Chaimongkol
authors.forename: Mariena
authors.initials: M
authors.lastname: Ketudat-Cairns
authors.forename: Rangsun
authors.initials: R
authors.lastname: Parnpai
mesh_terms: D000818:Animals; D016164:Bison / Q000196:embryology* / Q000235:genetics / Q000254:growth & development; D001755:Blastocyst / Q000187:drug effects; D002417:Cattle / Q000196:embryology* / Q000235:genetics / Q000254:growth & development; D002678:Chimera / Q000196:embryology* / Q000254:growth & development; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D005260:Female; D005313:Fetal Death / Q000662:veterinary; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008297:Male; D018895:Microsatellite Repeats / Q000187:drug effects; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D011247:Pregnancy; D047929:Term Birth; D058989:Vitrification
chemical_list: D006877:Hydroxamic Acids; C012589:trichostatin A