title: [The β-amylase polymorphism of winter common wheat grains].
abstract: The polymorphism of winter common wheat with respect to β-amylase isoenzymes has been analyzed using electrophoresis in polyacrylamide gel (PAAG) buffered with a Tris-glycine system (pH 8.3). Seven β-amylase isoenzymes have been found in wheat cultivars and the breeding stock. Isoenzymes A, B, and C are the most frequent in Russian and Ukrainian cultivars (51.7 4.7, 30.7 3.8, and 11.9 2.5%, respectively). Two alleles of the β-Amy-D1 locus of the long arm of chromosome 4D have been identified. The substrate-enzyme affine effect can be used to locate the zones of activity of this enzyme by means of staining for proteins. It has been determined that β-amylase zymotypes may play a role in the aggregating capacity of the grain protein complex via the formation of S-S bonds.
authors.forename: V P
authors.initials: VP
authors.lastname: Netsvetaev
authors.forename: O V
authors.initials: OV
authors.lastname: Akinshina
authors.forename: L S
authors.initials: LS
authors.lastname: Bondarenko
authors.forename: I P
authors.initials: IP
authors.lastname: Motorina
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics* / Q000378:metabolism; D056426:Genetic Loci / Q000502:physiology*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D014908:Triticum / Q000201:enzymology / Q000235:genetics*; D001614:beta-Amylase / Q000235:genetics* / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D001614:beta-Amylase