title: Response of Toll-like receptors in experimental Guillain-Barré syndrome: a kinetic analysis.
abstract: Guillain-Barré syndrome (GBS) is an autoimmune disorder caused by the interaction between cellular and humoral immune responses in the peripheral nervous system. Toll-like receptors (TLRs) are key players in innate and have regulatory functions in adaptive immunity. In this study, we systematically examined expression patterns of TLRs in sciatic nerve and lymphoid organs during the disease course of murine experimental autoimmune neuritis and in blood from Guillain-Barré patients. A kinetic response pattern was identified, characterized by a pronounced up-regulation of TLR2, 6 and 11 on T cells and TLR4 and 6 on APCs, while TLR1 expression was decreased. Moreover, an enhanced expression of the disease promoting cytokine Interleukin-(IL)17A was detected. Additional analysis of GBS patients revealed an up-regulation of TLR2, TLR4 and TLR6 mRNA, negatively correlated with disease severity. This first systematic analysis of TLR expression pattern may contribute to elucidating the role of TLRs in GBS pathophysiology.
authors.affiliation: Department of Neurology, Saarland University, 66421 Homburg/Saar, Germany.
authors.forename: Manuela
authors.initials: M
authors.lastname: Gries
authors.forename: Laura
authors.initials: L
authors.lastname: Davies
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Armin
authors.initials: A
authors.lastname: Bachhuber
authors.forename: Jörg
authors.initials: J
authors.lastname: Spiegel
authors.forename: Ulrich
authors.initials: U
authors.lastname: Dillmann
authors.forename: Tobias
authors.initials: T
authors.lastname: Hartmann
authors.forename: Klaus
authors.initials: K
authors.lastname: Fassbender
authors.forename: Silke
authors.initials: S
authors.lastname: Walter
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry; D020275:Guillain-Barre Syndrome / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D020381:Interleukin-17 / Q000032:analysis / Q000096:biosynthesis; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D053594:Myeloid Differentiation Factor 88 / Q000032:analysis / Q000096:biosynthesis; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012584:Sciatic Nerve / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D051193:Toll-Like Receptors / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D020381:Interleukin-17; D053594:Myeloid Differentiation Factor 88; D051193:Toll-Like Receptors