title: Occurrence of Trypanosoma caninum in areas overlapping with leishmaniasis in Brazil: what is the real impact of canine leishmaniasis control?
abstract: Trypanosoma caninum is a parasite of the Trypanosoma genus recently described in the natural infection of dogs in the municipality of Rio de Janeiro, Brazil. Suspecting the existence of a natural cycle and the circulation of this new species, the objective of this study was the taxonomic identification of samples of Trypanosoma spp. isolated from dogs in different Brazilian regions. Parasites were solely obtained from skin fragments culture and characterized by nested-PCR targeting the partial sequence of 18S rRNA gene and PCR products were sequenced. Thirty-three samples, obtained in São Paulo, Minas Gerais, Goiás, Mato Grosso and Rio de Janeiro states were analyzed. PCR and sequencing showed that the isolates were genetically identical or closely similar and confirmed T. caninum identity. This report broadens the geographical distribution of T. caninum in Brazil and discusses the impact of the presence of this parasite in areas of canine leishmaniasis occurrence.
authors.affiliation: Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ, Brasil. juliana.helena@ipec.fiocruz.br
authors.forename: J H S
authors.initials: JH
authors.lastname: Barros
authors.forename: A B P F
authors.initials: AB
authors.lastname: Almeida
authors.forename: F B
authors.initials: FB
authors.lastname: Figueiredo
authors.forename: V R F
authors.initials: VR
authors.lastname: Sousa
authors.forename: A
authors.initials: A
authors.lastname: Fagundes
authors.forename: A G S
authors.initials: AG
authors.lastname: Pinto
authors.forename: C
authors.initials: C
authors.lastname: Baptista
authors.forename: M F
authors.initials: MF
authors.lastname: Madeira
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D014355:Chagas Disease / Q000453:epidemiology / Q000517:prevention & control / Q000662:veterinary*; D003140:Communicable Disease Control; D004283:Dog Diseases / Q000453:epidemiology* / Q000517:prevention & control; D004285:Dogs; D019353:Endemic Diseases / Q000662:veterinary; D037901:Euthanasia, Animal; D006801:Humans; D018314:Leishmania infantum / Q000235:genetics / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000453:epidemiology / Q000517:prevention & control / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods; D012337:RNA, Ribosomal, 18S / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D014345:Trypanosoma / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012337:RNA, Ribosomal, 18S