title: Curcumin inhibits proliferation of human lens epithelial cells: a proteomic analysis.
abstract: OBJECTIVE
  The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells (LECs) is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) cells.
  
  
  METHODS
  Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO(2) incubator for 24 h.
  
  
  RESULTS
  We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower compared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF. Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge (m/z) ratios of 8093 and 13767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group.
  
  
  CONCLUSIONS
  These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8093 and 13767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation. Cur may be a reliable and effective drug for prevention and treatment of polymerase chain reaction (PCR).
authors.affiliation: Department of Ophthalmology, the Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, China. 12212022@163.com
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Hu
authors.forename: Xiu-rong
authors.initials: XR
authors.lastname: Huang
authors.forename: Ming-xin
authors.initials: MX
authors.lastname: Qi
authors.forename: Bu-yuan
authors.initials: BY
authors.lastname: Hou
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D020543:Proteome / Q000378:metabolism*
chemical_list: D020543:Proteome; D003474:Curcumin