title: Construction of a genetically engineered microorganism for phenanthrene biodegradation.
abstract: The bacterium Pseudomonas sp. CGMCC2953, isolated from oil-polluted soil, was used as a recipient for a biodegradative gene encoding catechol 2,3-dioxygenase (C23O), which was successfully cloned into the plasmid pK4 derived from pRK415 with a broad host range. The apparent phenanthrene biodegradation parameters of the recombinant microorganism (Pseudomonas sp. CGMCC2953-pK) were determined and compared with those of the wild type. As the key enzyme of phenanthrene degradation, C23O, could be expressed constitutively in the recombinant strain, Pseudomonas sp. CGMCC2953-pK showed an increased ability to degrade phenanthrene. The excessive production of C23O in Pseudomonas sp. CGMCC2953-pK could serve as an effective approach to construct genetically engineered microorganisms for the bioremediation of environmental contaminations.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biochemistry, East China University of Science and Technology, Shanghai, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jiashi
authors.initials: J
authors.lastname: Wei
authors.forename: Naimin
authors.initials: N
authors.lastname: Shao
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
mesh_terms: D001711:Biotransformation; D050557:Catechol 2,3-Dioxygenase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D060847:Metabolic Engineering; D010616:Phenanthrenes / Q000378:metabolism*; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D010616:Phenanthrenes; D011994:Recombinant Proteins; C031181:phenanthrene; D050557:Catechol 2,3-Dioxygenase