title: In vitro and in vivo anti-Staphylococcus aureus activities of a new disinfection system utilizing photolysis of hydrogen peroxide.
abstract: The present study aimed to evaluate in vitro and in vivo antibacterial activity of hydroxyl radical generation system by photolysis of H(2)O(2), which is a new disinfection system for the treatment of oral infection diseases such as periodontitis developed in our laboratory. Firstly, generation of the hydroxyl radical by the photolysis of H(2)O(2) in which 1 mol l(-1) H(2)O(2) was irradiated with a dual wavelength-light emitting diode (LED) at wavelengths of 400 and 465 nm was confirmed by applying an electron spin resonance-spin trapping technique. Secondly, the bactericidal effect of the system was examined under a similar condition in which Staphylococcus aureus suspended in 1 mol l(-1) H(2)O(2) was irradiated with LED light, resulting in substantial reduction of the colony forming unit (CFU) of the bacteria within a short time as 2 min. Finally, in vivo antibacterial effect of the photolysis of H(2)O(2) on a rat model of S. aureus infection was evaluated by a culture study. Since a significant reduction of recovered CFU of S. aureus was obtained, it is expected that in vitro antibacterial effect attributable to hydroxyl radicals generated by photolysis of H(2)O(2) could be well reflected in in vivo superficial bacterial infection.
authors.affiliation: Tohoku University Graduate School of Dentistry, Aoba-ku, Sendai, Japan.
authors.forename: Eisei
authors.initials: E
authors.lastname: Hayashi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Mokudai
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Yamada
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nakamura
authors.forename: Taro
authors.initials: T
authors.lastname: Kanno
authors.forename: Keiichi
authors.initials: K
authors.lastname: Sasaki
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Niwano
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004203:Disinfection / Q000379:methods*; D004578:Electron Spin Resonance Spectroscopy; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000528:radiation effects*; D017665:Hydroxyl Radical / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D010782:Photolysis / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000382:microbiology / Q000628:therapy; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide