title: Drosophila heparan sulfate, a novel design.
abstract: Heparan sulfate (HS) proteoglycans play critical roles in a wide variety of biological processes such as growth factor signaling, cell adhesion, wound healing, and tumor metastasis. Functionally important interactions between HS and a variety of proteins depend on specific structural features within the HS chains. The fruit fly (Drosophila melanogaster) is frequently applied as a model organism to study HS function in development. Previous structural studies of Drosophila HS have been restricted to disaccharide composition, without regard to the arrangement of saccharide domains typically found in vertebrate HS. Here, we biochemically characterized Drosophila HS by selective depolymerization with nitrous acid. Analysis of the generated saccharide products revealed a novel HS design, involving a peripheral, extended, presumably single, N-sulfated domain linked to an N-acetylated sequence contiguous with the linkage to core protein. The N-sulfated domain may be envisaged as a heparin structure of unusually low O-sulfate content.
authors.affiliation: Department of Biomedicine, University of Bergen, NO-5009 Bergen, Norway. marion.kusche@biomed.uib.no
authors.forename: Marion
authors.initials: M
authors.lastname: Kusche-Gullberg
authors.forename: Kent
authors.initials: K
authors.lastname: Nybakken
authors.forename: Norbert
authors.initials: N
authors.lastname: Perrimon
authors.forename: Ulf
authors.initials: U
authors.lastname: Lindahl
mesh_terms: D000818:Animals; D000838:Anions; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004187:Disaccharides / Q000737:chemistry; D004330:Drosophila / Q000378:metabolism*; D004586:Electrophoresis / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D006025:Glycosaminoglycans / Q000737:chemistry; D019812:Heparan Sulfate Proteoglycans / Q000737:chemistry*; D006497:Heparan Sulfate / Q000737:chemistry*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship
chemical_list: D000838:Anions; D004187:Disaccharides; D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate