title: Manipulation and capture of Aβ amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP).
abstract: Here we report a novel method for the manipulation and concentration of Aβ amyloid fibrils, implicated in Alzheimer's disease, using DC insulating gradient dielectrophoresis (DC-iGDEP). Fibril enrichment was found to be ∼400%. Simulations suggest that capture of the full range of amyloid protein aggregates is possible with optimized device design.
authors.affiliation: Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ, USA.
authors.forename: Sarah J R
authors.initials: SJ
authors.lastname: Staton
authors.forename: Paul V
authors.initials: PV
authors.lastname: Jones
authors.forename: Ginger
authors.initials: G
authors.lastname: Ku
authors.forename: S Douglass
authors.initials: SD
authors.lastname: Gilman
authors.forename: Indu
authors.initials: I
authors.lastname: Kheterpal
authors.forename: Mark A
authors.initials: MA
authors.lastname: Hayes
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000302:isolation & purification*; D004553:Electric Conductivity; D017097:Electric Impedance; D004586:Electrophoresis / Q000379:methods*; D055503:Protein Multimerization; D017433:Protein Structure, Secondary
chemical_list: D016229:Amyloid beta-Peptides