title: Quantitative analysis of the redox states of cytochromes in a living L929 (NCTC) cell by resonance Raman microspectroscopy.
abstract: Raman spectra and images of a living L929 (NCTC) cell have been measured with 532 nm excitation. Both reduced and oxidized forms of cytochromes b and c (cyt b and cyt c) have been observed in situ without any pretreatment. The redox states of cyts b and c have been assessed quantitatively with a spectral analysis. It has been found that reduced cyt c is more abundant than oxidized cyt c, while oxidized cyt b is slightly more abundant than reduced cyt b in a living cell.
authors.affiliation: Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo, Toyo 113-0033, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Kakita
authors.forename: Masanari
authors.initials: M
authors.lastname: Okuno
authors.forename: Hiro-o
authors.initials: HO
authors.lastname: Hamaguchi
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival; D045303:Cytochromes b / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism*; D006801:Humans; D051379:Mice; D015591:Microspectrophotometry / Q000379:methods*; D010084:Oxidation-Reduction; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D045304:Cytochromes c; D045303:Cytochromes b