title: The manipulation of natural killer cells to target tumor sites using magnetic nanoparticles.
abstract: The present work demonstrates that Cy5.5 conjugated Fe(3)O(4)/SiO(2) core/shell nanoparticles could allow us to control movement of human natural killer cells (NK-92MI) by an external magnetic field. Required concentration of the nanoparticles for the cell manipulation is as low as ~20 μg Fe/mL. However, the relative ratio of the nanoparticles loaded NK-92MI cells infiltrated into the target tumor site is enhanced by 17-fold by applying magnetic field and their killing activity is still maintained as same as the NK-92MI cells without the nanoparticles. This approach allows us to open alternative clinical treatment with reduced toxicity of the nanoparticles and enhanced infiltration of immunology to the target site.
authors.affiliation: Molecular Imaging Program at Stanford (MIPS) and Bio-X Program, Department of Radiology, Stanford University, 1201 Welch Rd, Stanford, CA 94305, USA.
authors.forename: Eue-Soon
authors.initials: ES
authors.lastname: Jang
authors.forename: June-Ho
authors.initials: JH
authors.lastname: Shin
authors.forename: Gang
authors.initials: G
authors.lastname: Ren
authors.forename: Mi-Jin
authors.initials: MJ
authors.lastname: Park
authors.forename: Kai
authors.initials: K
authors.lastname: Cheng
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
authors.forename: Joseph C
authors.initials: JC
authors.lastname: Wu
authors.forename: John B
authors.initials: JB
authors.lastname: Sunwoo
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000528:radiation effects; D018189:Immunomagnetic Separation / Q000379:methods*; D007694:Killer Cells, Natural / Q000528:radiation effects* / Q000637:transplantation*; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles; D009374:Neoplasms, Experimental / Q000473:pathology* / Q000628:therapy*; D051381:Rats; D016896:Treatment Outcome
chemical_list: D058185:Magnetite Nanoparticles