title: Enhancement of endothelial cell migration by constitutively active LPA(1)-expressing tumor cells.
abstract: Lysophosphatidic acid (LPA) receptors belong to G protein-coupled transmembrane receptors (LPA receptors; LPA(1) to LPA(6)). They indicate a variety of cellular response by the interaction with LPA, including cell proliferation, migration and differentiation. Recently, we have reported that constitutive active mutated LPA(1) induced the strong biological effects of rat neuroblastoma B103 cells. In the present study, we examined the effects of mutated LPA(1) on the interaction between B103 cells and endothelial F-2 cells. Each LPA receptor expressing B103 cells were maintained in serum-free DMEM and cell motility assay was performed with a Cell Culture Insert. When F-2 cells were cultured with conditioned medium from Lpar1 and Lpar3-expressing cells, the cell motility of F-2 cells was significantly higher than control cells. Interestingly, the motile activity of F-2 cells was strongly induced by mutated LPA(1) than other cells, correlating with the expression levels of vascular endothelial growth factor (Vegf)-A and Vegf-C. Pretreatment of LPA signaling inhibitors inhibited F-2 cell motility stimulated by mutated LPA(1). These results suggest that activation of LPA signaling via mutated LPA(1) may play an important role in the promotion of angiogenesis in rat neuroblastoma cells.
authors.affiliation: Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502, Japan.
authors.forename: Misaho
authors.initials: M
authors.lastname: Kitayoshi
authors.forename: Kohei
authors.initials: K
authors.lastname: Kato
authors.forename: Eriko
authors.initials: E
authors.lastname: Tanabe
authors.forename: Kyohei
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Rie
authors.initials: R
authors.lastname: Fukui
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fukushima
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Tsujiuchi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D009154:Mutation; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology*; D009447:Neuroblastoma / Q000098:blood supply*; D051381:Rats; D049368:Receptors, Lysophosphatidic Acid / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics
chemical_list: D049368:Receptors, Lysophosphatidic Acid; D042461:Vascular Endothelial Growth Factor A; D042582:Vascular Endothelial Growth Factor C; C467498:vascular endothelial growth factor A, rat