title: A novel thermotolerant and acidotolerant peptide produced by a Bacillus strain newly isolated from a fermented food (kimchi) shows activity against multidrug-resistant bacteria.
abstract: In an attempt to isolate effective antimicrobial peptides (AMPs) from a microbial source for the treatment of multidrug-resistant (MDR) bacteria, BCP61 was purified from Bacillus sp. CS61 newly isolated from the traditional fermented food kimchi. BCP61 (ca. 1100 Da) was purified to homogeneity using sequential chromatographic steps. It was found to be stable at pH 2.0-10.0 and up to 80 °C. BCP61 displayed antimicrobial activity against MDR bacteria such as meticillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant S. aureus (VRSA) and vancomycin-resistant enterococci (VRE). Minimal inhibitory concentrations of BCP61 for MRSA, VRSA and VRE were in the range 0.625-20μg/mL. The N-terminal amino acid sequence of BCP61 was A-I-N-X-D-A-A-Y-L, which differed from reported AMPs. The fourth unidentified amino acid was replaced and several peptides were synthesised. Among them, only cysteine replacement displayed antimicrobial activity. BCP61 from a food-borne strain may be useful in therapeutic applications.
authors.affiliation: Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju 501-759, South Korea.
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Choi
authors.forename: Seung Sik
authors.initials: SS
authors.lastname: Cho
authors.forename: Jaya Ram
authors.initials: JR
authors.lastname: Simkhada
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Yoo
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry* / Q000302:isolation & purification; D001419:Bacteria; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002845:Chromatography / Q000379:methods; D016983:Enterococcus / Q000187:drug effects; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D055550:Protein Stability; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; C000617975:BCP61 peptide, Bacillus; D001426:Bacterial Proteins; D001688:Biological Products; D010455:Peptides