title: Synthesis and synergistic antifungal activities of a pyrazoline based ligand and its copper(II) and nickel(II) complexes with conventional antifungals.
abstract: A pyrazoline based ligand; (5-(4-chlorophenyl)-3-phenyl-4, 5-dihydro-1H-pyrazole-1-carbothioamide) has been synthesized by Claisen-Schmidt condensation of acetophenone with p-chlorobenzaldehyde, followed by sodium hydroxide assisted cyclization of the resulting chalcone with thiosemicarbazide. Metal ion complexes of the synthesized ligand were prepared with Cu(II) and Ni(II) metal ions, separately and respectively. Ligand and the metal complexes were characterized by elemental analysis, FT-IR, UV-Vis, (1)HNMR, ESI-MS and (13)CNMR spectroscopic techniques. Molar conductance measurements in DMSO suggested non-electrolytic nature of the complexes. Tetragonally distorted octahedral geometry for copper and octahedral geometry for the nickel complexes was proposed on the basis of UV-Vis spectroscopic studies and magnetic moment measurements. The complexes were investigated for their ability to kill human fungal pathogen Candida by determining MICs (Minimum inhibitory concentrations), inhibition in solid media and ability to produce a possible synergism with conventional most clinically practiced antifungals by disc diffusion assay and FICI (fractional inhibitory concentration index).
authors.affiliation: Department of Chemistry, Jamia Millia Islamia, New Delhi 110025, India. drimran_ali@yahoo.com
authors.forename: Imran
authors.initials: I
authors.lastname: Ali
authors.forename: Waseem A
authors.initials: WA
authors.lastname: Wani
authors.forename: Amber
authors.initials: A
authors.lastname: Khan
authors.forename: Ashanul
authors.initials: A
authors.lastname: Haque
authors.forename: Aijaz
authors.initials: A
authors.lastname: Ahmad
authors.forename: Kishwar
authors.initials: K
authors.lastname: Saleem
authors.forename: Nikhat
authors.initials: N
authors.lastname: Manzoor
mesh_terms: D000935:Antifungal Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects; D002177:Candidiasis / Q000382:microbiology; D003300:Copper / Q000737:chemistry / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D008024:Ligands; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009532:Nickel / Q000737:chemistry / Q000494:pharmacology*; D011720:Pyrazoles / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D008024:Ligands; D011720:Pyrazoles; D003300:Copper; D009532:Nickel