title: Engineering the third wave of biocatalysis.
abstract: Over the past ten years, scientific and technological advances have established biocatalysis as a practical and environmentally friendly alternative to traditional metallo- and organocatalysis in chemical synthesis, both in the laboratory and on an industrial scale. Key advances in DNA sequencing and gene synthesis are at the base of tremendous progress in tailoring biocatalysts by protein engineering and design, and the ability to reorganize enzymes into new biosynthetic pathways. To highlight these achievements, here we discuss applications of protein-engineered biocatalysts ranging from commodity chemicals to advanced pharmaceutical intermediates that use enzyme catalysis as a key step.
authors.affiliation: Institute of Biochemistry, Department of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Straße 4, D-17487 Greifswald, Germany. uwe.bornscheuer@uni-greifswald.de
authors.forename: U T
authors.initials: UT
authors.lastname: Bornscheuer
authors.forename: G W
authors.initials: GW
authors.lastname: Huisman
authors.forename: R J
authors.initials: RJ
authors.lastname: Kazlauskas
authors.forename: S
authors.initials: S
authors.lastname: Lutz
authors.forename: J C
authors.initials: JC
authors.lastname: Moore
authors.forename: K
authors.initials: K
authors.lastname: Robins
mesh_terms: D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods / Q000639:trends; D019295:Computational Biology / Q000379:methods / Q000639:trends; D019020:Directed Molecular Evolution; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism*; D055772:Green Chemistry Technology; D015202:Protein Engineering / Q000379:methods / Q000639:trends
chemical_list: D004798:Enzymes