title: NMR characterization of the interaction between the PUB domain of peptide:N-glycanase and ubiquitin-like domain of HR23.
abstract: PUB domains are identified in several proteins functioning in the ubiquitin (Ub)-proteasome system and considered as p97-binding modules. To address the further functional roles of these domains, we herein characterized the interactions of the PUB domain of peptide:N-glycanase (PNGase) with Ub and Ub-like domain (UBL) of the proteasome shuttle factor HR23. NMR data indicated that PNGase-PUB exerts an acceptor preferentially for HR23-UBL, electrostatically interacting with the UBL surface employed for binding to other Ub/UBL motifs. Our findings imply that PNGase-PUB serves not only as p97-binding module but also as a possible activator of HR23 in endoplasmic reticulum-associated degradation mechanisms.
authors.affiliation: Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Aichi, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kamiya
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Uekusa
authors.forename: Akira
authors.initials: A
authors.lastname: Sumiyoshi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sasakawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hirao
authors.forename: Tadashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
mesh_terms: D001665:Binding Sites; D045643:DNA Repair Enzymes / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000737:chemistry* / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D017434:Protein Structure, Tertiary; D025801:Ubiquitin / Q000737:chemistry* / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes / Q000737:chemistry / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; C513287:RAD23B protein, human; D025801:Ubiquitin; C086985:RAD23A protein, human; C100879:UBE2K protein, human; D044763:Ubiquitin-Conjugating Enzymes; D046988:Proteasome Endopeptidase Complex; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D045643:DNA Repair Enzymes