title: Genome shuffling of Clostridium acetobutylicum CICC 8012 for improved production of acetone-butanol-ethanol (ABE).
abstract: Genome shuffling was applied to increase ABE production of the strict anaerobe C. acetobutylicum CICC 8012. By using physical and chemical mutagenesis, strains with superior streptomycin sulfate, 2-deoxy-D-glucose and butanol tolerance levels were isolated. These strains were used for genome shuffling. The best performing strain F2-GA was screened after two rounds of genome shuffling. With 55 g glucose/l as carbon source, F2-GA produced 22.21 g ABE/l in 72 h and ABE yield reached 0.42 g/g which was about 34.53 % improvement compared to the wild type. Fermentation parameters and gene expression of several key enzymes in ABE metabolic pathways were varied significantly between F2-GA and the wild type. These results demonstrated the potential use of genome shuffling to microbial breeding which were difficult to deal with traditional methods.
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, China. xiaofenggao1981@163.com
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Gao
authors.forename: Hai
authors.initials: H
authors.lastname: Zhao
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhang
authors.forename: Kaize
authors.initials: K
authors.lastname: He
authors.forename: Yanling
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000096:Acetone / Q000378:metabolism*; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000235:genetics* / Q000378:metabolism*; D032621:DNA Shuffling; D004269:DNA, Bacterial / Q000235:genetics*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D016680:Genome, Bacterial; D060847:Metabolic Engineering / Q000379:methods
chemical_list: D000440:Butanols; D004269:DNA, Bacterial; D000096:Acetone; D000431:Ethanol