title: Biomimetic engineering of a generic cell-on-membrane architecture by microfluidic engraving for on-chip bioassays.
abstract: We develop a biomimetic cell-on-membrane architecture in close-volume format which allows the interfacial biocompatibility and the reagent delivery capability for on-chip bioassays. The key concept lies in the microfluidic engraving of lipid membranes together with biological cells on a supported substrate with topographic patterns. The simultaneous engraving process of a different class of fluids is promoted by the front propagation of an air-water interface inside a flow-cell. This highly parallel, microfluidic cell-on-membrane approach opens a door to the natural biocompatibility in mimicking cellular stimuli-response behavior essential for diverse on-chip bioassays that can be precisely controlled in the spatial and temporal manner.
authors.affiliation: School of Electrical Engineering #032, Seoul National University, Kwanak P.O. Box 34, Seoul 151-600, South Korea.
authors.forename: Sang-Wook
authors.initials: SW
authors.lastname: Lee
authors.forename: Ji-Yoon
authors.initials: JY
authors.lastname: Noh
authors.forename: Seung Chul
authors.initials: SC
authors.lastname: Park
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Byoungho
authors.initials: B
authors.lastname: Lee
authors.forename: Sin-Doo
authors.initials: SD
authors.lastname: Lee
mesh_terms: D000293:Adolescent; D000328:Adult; D057005:Bioengineering / Q000379:methods*; D001681:Biological Assay / Q000379:methods*; D032701:Biomimetics / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism*; D060833:Cellular Microenvironment; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D055815:Young Adult
chemical_list: D008051:Lipid Bilayers