title: Association of CLCNKB haplotypes and hypertension in Mongolian and Han populations.
abstract: We investigated a possible association between genetic variations in chloride channel Kb (CLCNKB) gene and essential hypertension (EH) in the Mongolian and Han populations in Inner Mongolia. Our study included 414 unrelated Mongolian herdsmen and 524 Han farmers. Two tagSNPs of CLCNKB (rs945393 and rs10803414) were identified from the Chinese HapMap database based on pairwise r(2) ≥ 0.5 and minor allele frequency ≥0.05. Genotyping was performed using the PCR/ligase detection reaction assay. There was significant difference in allele frequency of rs10803414 between the EH group (35%) and the control group (26%) in the Mongolian population (P < .05). Significant association was identified between rs10803414 and EH in the Mongolian population (P < .05) and rs945393 and EH in the Han population (P < .01). The frequency of haplotype CC in the EH group (9.4%) was significantly higher than in the control group (4.6%) in the Mongolian population; individuals who possessed the CC haplotype had a significantly higher risk of EH in the Mongolian population. There was no association between haplotype and EH in the Han population. After adjusting for age, sex, and other confounding risk factors, only rs10803414 was the risk factor of hypertension in Mongolians. Our results indicate that rs10803414 in CLCNKB confers a significant risk of EH in the Mongolian population and haplotype CC of CLCNKB is a genetic factor for EH in the Mongolian population. Our study expands the association between CLCNKB and EH to a non-European ancestry population and provides the first evidence of a cross-race susceptibility of EH locus.
authors.affiliation: Clinical Medical Research Center of the Affiliated Hospital, Inner Mongolia Medical College, Hohhot, China. xlsu@hotmail.com
authors.forename: Xiulan
authors.initials: X
authors.lastname: Su
authors.forename: Peiye
authors.initials: P
authors.lastname: Chang
authors.forename: Zhiyue
authors.initials: Z
authors.lastname: Liu
authors.forename: Meirong
authors.initials: M
authors.lastname: Yan
authors.forename: Guirong
authors.initials: G
authors.lastname: Liu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Cui
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D018118:Chloride Channels / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D008986:Mongolia; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012307:Risk Factors; D055815:Young Adult
chemical_list: C489932:CLCNKA protein, human; D018118:Chloride Channels