title: Antibacterial carbazole alkaloids from Clausena harmandiana twigs.
abstract: Three new carbazole alkaloids, harmandianamines A-C (1-3), together with fifteen known compounds (4-18) were isolated from the twigs of Clausena harmandiana. The structures were elucidated by spectroscopic methods, including UV, IR, NMR, and MS. The antibacterial activity against Escherichia coli TISTR 780, Salmonella typhimurium TISTR 292, Staphylococcus aureus TISTR 1466 and methicillin-resistant S. aureus (MRSA) SK1 of some isolated compounds was also evaluated. Compound 6 exhibited significant antibacterial activity against MRSA SK1 with an MIC value of 0.25 μg/mL which higher than that of standard drug, vancomycin (MIC value=1 μg/mL) whereas compounds 14 and 5 showed strong activity with MIC values of 4 and 8 μg/mL, respectively. Only compound 14 showed strong antibacterial activity against S. aureus TISTR 1466 with an MIC value of 4 μg/mL.
authors.affiliation: Natural Products Research Laboratory, School of Science, Mae Fah Luang University, Tasud, Muang, Chiang Rai 57100, Thailand.
authors.forename: Wisanu
authors.initials: W
authors.lastname: Maneerat
authors.forename: Wong
authors.initials: W
authors.lastname: Phakhodee
authors.forename: Thunwadee
authors.initials: T
authors.lastname: Ritthiwigrom
authors.forename: Sarot
authors.initials: S
authors.lastname: Cheenpracha
authors.forename: Trinop
authors.initials: T
authors.lastname: Promgool
authors.forename: Kulsiri
authors.initials: K
authors.lastname: Yossathera
authors.forename: Suwanna
authors.initials: S
authors.lastname: Deachathai
authors.forename: Surat
authors.initials: S
authors.lastname: Laphookhieo
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002227:Carbazoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D054700:Clausena / Q000737:chemistry*; D026121:Indole Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016106:Methicillin Resistance / Q000187:drug effects; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D002227:Carbazoles; D026121:Indole Alkaloids; D010936:Plant Extracts; D014640:Vancomycin