title: Elastic chitosan conduits with multiple channels and well defined microstructure.
abstract: Four kinds of chitosan conduits with longitudinal multi-channels and controlled internal microstructures were prepared using a special mold and a freeze-drying method. One of the conduits was fabricated from a chitosan solution (ab NC), while the other three groups were made from a pre-gelled chitosan solution using genipin as a chemical cross-linker (ab gNC), dibasic sodium phosphate as a physical cross-linker (ab pNC) or a combined ionic and covalent co-cross-linker (ab gpNC), respectively. The porosity of the chitosan conduits ranged from 88 to 90%. The gpNC showed highly interconnected and uniformly distributed pores compared to NC, the gNC and pNC. In contrast, the gNC and gpNC showed about 10% of the volume swelling ratio in 37°C PBS solution, although the gpNC scaffold's water uptake was the highest, at more than 17 times its original mass. Compressive tests showed that gpNC had significant elasticity and maintained its physical integrity even after compressing them down to 20% of their original height. The elastic modulus of gpNC reached 80 kPa, which was more than twice that of the other groups. Adhesion and proliferation of PC12 cells on chitosan gpNC scaffolds showed excellent properties by MTT and SEM observation, which indicated the potential of gpNC scaffolds for nerve tissue engineering applications.
authors.affiliation: DSAPM Laboratory, PCFM Laboratory, Institute of Polymer Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, China.
authors.forename: Jixiang
authors.initials: J
authors.lastname: Zhu
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiong
authors.forename: Chenguang
authors.initials: C
authors.lastname: Zeng
authors.forename: Na
authors.initials: N
authors.lastname: Qiang
authors.forename: Daping
authors.initials: D
authors.lastname: Quan
authors.forename: Jun
authors.initials: J
authors.lastname: Wan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D048271:Chitosan / Q000737:chemistry*; D004548:Elasticity; D039823:Iridoids / Q000737:chemistry; D008422:Materials Testing; D016716:PC12 Cells; D016062:Porosity; D051381:Rats; D012996:Solutions / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D039823:Iridoids; D012996:Solutions; D048271:Chitosan; C007834:genipin