title: Simultaneous determination of diclofenac potassium and drotaverine hydrochloride in human plasma using reversed-phase high-performance liquid chromatography.
abstract: A simple high-performance liquid chromatographic method with ultraviolet detection is proposed for the estimation of diclofenac potassium and drotaverine hydrochloride in human plasma. Liquid-liquid extraction was carried out with a mixture of dichloromethane-isopropyl alcohol (80:20, v/v). Chromatographic separation of the analytes and internal standard was achieved on an analytical 250 × 4.6 mm i.d. reversed-phase Thermo BDS Hypersil C8 (5 µm particle size) column using a mobile phase of acetonitrile-0.02M ammonium acetate buffer (53:47, v/v) at pH 3.5. The run time was less than 15 min. Column eluate was monitored at 230 nm. The linearity over the concentration ranges of 25-1500 ng/mL and 32-960 ng/mL was obtained for diclofenac potassium and drotaverine hydrochloride, respectively. The limit of quantification was 25 and 32 ng/mL for diclofenac potassium and drotaverine hydrochloride, respectively. Recoveries of diclofenac potassium and drotaverine hydrochloride from plasma were 97.45% and 98.27%, respectively.
authors.affiliation: Department of Pharmaceutical Analysis, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, India. raj17579@rediffmail.com
authors.forename: Prasad P
authors.initials: PP
authors.lastname: Dahivelkar
authors.forename: Suvarna I
authors.initials: SI
authors.lastname: Bhoir
authors.forename: Sanjay B
authors.initials: SB
authors.lastname: Bari
authors.forename: Sanjay J
authors.initials: SJ
authors.lastname: Surana
authors.forename: Ashok M
authors.initials: AM
authors.lastname: Bhagwat
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004008:Diclofenac / Q000097:blood* / Q000737:chemistry; D006801:Humans; D016018:Least-Squares Analysis; D059625:Liquid-Liquid Extraction; D010208:Papaverine / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004008:Diclofenac; C005317:drotaverin; D010208:Papaverine