title: Evaluation of a pretreatment method for two-dimensional gel electrophoresis of synovial fluid using cartilage oligomeric matrix protein as a marker.
abstract: Osteoarthritis (OA) is the most common rheumatic pathology. One of the major objectives of OA research is the development of early diagnostic strategies such as those using proteomic technology. Synovial fluid (SF) in OA patients is a potential source of biomarkers for OA. The efficient and reliable preparation of SF proteomes is a critical step towards biomarker discovery. In this study, we have optimized a pretreatment method for two-dimensional gel electrophoresis (2DE) separation of the SF proteome, by enriching low-abundance proteins and simultaneously removing hyaluronic acid, albumin, and IgG. SF samples pretreated using this optimized method were then evaluated by 1DE and 2DE separation followed by immunodetection of cartilage oligomeric matrix protein (COMP), a known OA biomarker, and by the identification of 3 proteins (apolipoprotein, haptoglobin precursor, and fibrinogen D fragment) that are related to joint diseases.
authors.affiliation: Department of Orthopedic Surgery, Cell Therapy Center, Ajou University, Suwon 443-749, Korea.
authors.forename: Min Kyung
authors.initials: MK
authors.lastname: Kong
authors.forename: Byoung Hyun
authors.initials: BH
authors.lastname: Min
authors.forename: Pyung Cheon
authors.initials: PC
authors.lastname: Lee
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D064236:Cartilage Oligomeric Matrix Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D016326:Extracellular Matrix Proteins / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008297:Male; D064235:Matrilin Proteins; D008875:Middle Aged; D010003:Osteoarthritis / Q000175:diagnosis / Q000378:metabolism*; D040901:Proteomics; D013048:Specimen Handling / Q000379:methods*; D013582:Synovial Fluid / Q000737:chemistry* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D064236:Cartilage Oligomeric Matrix Protein; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins; C577553:TSP5 protein, human