title: Chloroplastic NADPH-dependent alkenal/one oxidoreductase contributes to the detoxification of reactive carbonyls produced under oxidative stress.
abstract: Lipid peroxide-derived reactive carbonyls (RCs) can cause serious damage to plant functions. A chloroplastic NADPH-dependent alkenal/one oxidoreductase (AOR) detoxifies RCs, but its physiological significance remains unknown. In this study, we investigated the biological impacts of AOR using an AOR-knock out Arabidopsis line (aor). Methyl viologen treatment, mainly to enhance photosystem (PS) I-originated reactive oxygen species (ROS) production, caused more severe damage to aor than wild type (Col-0). In contrast, the high light treatment used to enhance PSII-originated ROS production resulted in no difference in PSII damage between Col-0 and aor. In conclusion, AOR can contribute to detoxify stromal RCs produced under oxidative stress.
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan. yamauchi@kobe-u.ac.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yamauchi
authors.forename: Ayaka
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Masaharu
authors.initials: M
authors.lastname: Mizutani
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Sugimoto
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology / Q000378:metabolism*; D055786:Gene Knockout Techniques; D018384:Oxidative Stress; D050837:Oxidoreductases Acting on Aldehyde or Oxo Group Donors / Q000235:genetics / Q000378:metabolism*; D045331:Photosystem I Protein Complex / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D045331:Photosystem I Protein Complex; D017382:Reactive Oxygen Species; D000429:Alcohol Oxidoreductases; C082646:leukotriene B4 12-hydroxydehydrogenase; C000618213:AOR protein, Arabidopsis; D050837:Oxidoreductases Acting on Aldehyde or Oxo Group Donors