title: [Simultaneous determination of trillin and desgalactotigonin contents in Solanum lyratum by HPLC-ELSD].
abstract: OBJECTIVE
  To establish a HPLC-ELSD method for simultaneous determination of trillin and desgalactotigonin contents in Solanum lyratum.
  
  
  METHODS
  A Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was adopted, with the mobile phase consisting of acetonitrile-10 mmol x L(-1) ammonium acetate (52: 48). The temperature was 25 degrees C, the flow rate was set at 0.6 mL x min(-1), and the sample size is 20 microL. The temperature of drift tubes and gas flow rate of the detector were set at 95 degrees C and 2.3 L x min(-1), respectively.
  
  
  RESULTS
  With in the linear ranges of 20-200 mg x L(-1) and 10-100 mg x L(-1), trillin and desgalactotigonin show a good linear relationship. The average recovery was 99.4% (RSD 0.90%) for trillin and 100.3% (RSD 1.1%) for desgalactotigonin.
  
  
  CONCLUSIONS
  The method is so accurate and easily reproducible that it is suitable for the quality control of S. lyratum medicinal materials.
authors.affiliation: College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016. jiayurong8511@163.com
authors.forename: Yurong
authors.initials: Y
authors.lastname: Jia
authors.forename: Changshan
authors.initials: C
authors.lastname: Sun
authors.forename: Yanxiu
authors.initials: Y
authors.lastname: Sun
authors.forename: Wenwen
authors.initials: W
authors.lastname: Yang
authors.forename: Lixin
authors.initials: L
authors.lastname: Sun
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D032322:Solanum / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal