title: Proteomic analysis of the impact of static culturing on the expansion of rat bone marrow mesenchymal stem cells.
abstract: The clinical potential of mesenchymal stem cells (MSC) in tissue engineering and regenerative medicine is due to their self-renewal, proliferation and multi-lineage differentiation potential. Clinical use requires large cell numbers; which can, theoretically, be generated by ex vivo expansion of plastic adherent, MSC subpopulation, of bone marrow cells (BMC). Effects of serial culture on MSC phenotype were investigated using non-gel based quantitative proteomic methodology for static monolayer cultures of rat BMC. In total, 382 proteins were relatively quantified (≥ 2 peptides). Nine proteins were up-regulated and seven down-regulated at passage 4 relative to passage 2 (p ≤ 0.05). We propose that serial culture impacts on MSC expansion (observed decline in colony forming potential and colony size) is through a combination of osteogenic differentiation and ageing/senescence and propose six novel protein biomarkers as candidates for quality control purposes in bioprocessing.
authors.affiliation: Academic Urology Unit and Institute for Cancer Studies, Medical School, The University of Sheffield, Beech Hill Road, Sheffield, S10 2RX, UK. stefan.peter@sheffield.ac.uk
authors.forename: Stefan
authors.initials: S
authors.lastname: Peter
authors.forename: Caroline
authors.initials: C
authors.lastname: Evans
authors.forename: Saw Y
authors.initials: SY
authors.lastname: Ow
authors.forename: Andy M
authors.initials: AM
authors.lastname: Scutt
authors.forename: Phillip C
authors.initials: PC
authors.lastname: Wright
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Biggs
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D016922:Cellular Senescence; D007553:Isotope Labeling; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010012:Osteogenesis; D010641:Phenotype; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D051381:Rats
chemical_list: D015415:Biomarkers; D020543:Proteome