title: Strategies for enrichment and selection of stem cell-derived tissue precursors.
abstract: Human embryonic stem cells have the capacity for self-renewal and pluripotency and thus are a primary candidate for tissue engineering and regenerative therapies. These cells also provide an opportunity to study the development of human tissues ex vivo. To date, numerous human embryonic stem cell lines have been derived and characterized. In this review, we will detail the strategies used to direct tissue-specific differentiation of embryonic stem cells. We also will discuss how these strategies have produced new sources of tissue-specific progenitor cells. Finally, we will describe the next generation of methods being developed to identify and select stem cell-derived tissue precursors for experimental study and clinical use.
authors.affiliation: Department of Pediatrics (Cardiology), University of California San Francisco, San Francisco, CA 94143-1346, USA. harold.bernstein@ucsf.edu
authors.forename: Harold S
authors.initials: HS
authors.lastname: Bernstein
authors.forename: William C
authors.initials: WC
authors.lastname: Hyun
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry*; D053595:Embryonic Stem Cells / Q000737:chemistry / Q000166:cytology*; D044127:Epigenesis, Genetic; D005434:Flow Cytometry / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005855:Germ Layers / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D039904:Pluripotent Stem Cells / Q000737:chemistry / Q000166:cytology*; D011956:Receptors, Cell Surface / Q000737:chemistry
chemical_list: D003470:Culture Media; D011956:Receptors, Cell Surface