title: Recombinant glycoprotein 63 (Gp63) of Trypanosoma carassii suppresses antimicrobial responses of goldfish (Carassius auratus L.) monocytes and macrophages.
abstract: We previously reported that proteins secreted by Trypanosoma carassii play a role in evasion of fish host immune responses. To further understand how these parasites survive in the host, we cloned and expressed T. carassii glycoprotein 63 (Tcagp63), and generated a rabbit polyclonal antibody to the recombinant protein (rTcagp63). Tcagp63 was similar to gp63 of other trypanosomes and grouped with Trypanosoma cruzi and Trypanosoma brucei gp63 in phylogenetic analysis. We showed that rTcagp63 down-regulated Aeromonas salmonicida and recombinant goldfish TNFα2-induced production of reactive oxygen and nitrogen intermediates. Macrophages treated with rTcagp63 also exhibited significant reduction in the expression of inducible nitric oxide synthase (iNOS)-A, TNFα-1 and TNFα-2. Recombinant Tcagp63 bound to and was internalised by goldfish macrophages. The Tcagp63 may act by altering the signalling events important in downstream monocyte/macrophage antimicrobial and other cytokine-induced functions. We believe that this is the first report on downregulation of antimicrobial responses by trypanosome gp63.
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Ayoola
authors.initials: A
authors.lastname: Oladiran
authors.forename: Miodrag
authors.initials: M
authors.lastname: Belosevic
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006054:Goldfish / Q000276:immunology* / Q000469:parasitology; D006790:Host-Parasite Interactions; D057131:Immune Evasion; D008264:Macrophages / Q000276:immunology* / Q000469:parasitology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology* / Q000469:parasitology; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D026361:Reactive Nitrogen Species / Q000276:immunology / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000276:immunology / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014345:Trypanosoma / Q000276:immunology / Q000472:pathogenicity*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D016054:DNA, Protozoan; D006023:Glycoproteins; D015800:Protozoan Proteins; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D037521:Virulence Factors