title: Algal fucoidan, unlike heparin, has thrombolytic activity in a murine arterial thrombosis model.
abstract: Thrombolytic effects of fucoidans were investigated in the FeCl3-induced arterial thrombus mouse model and compared with heparin and tissue plasminogen activator (t-PA). Thrombosis model was made by applying 5% FeCl3 on the carotid artery of a Balb/c mouse. Twenty minutes after complete occlusion, a couple of test agents including fucoidan were infused into each mouse group with various doses intravenously, before measuring the time to reperfusion. The occluded arteries were reperfused 37.5 ± 12.4 min after administration of unfractionated fucoidan from Undaria pinnatifida sporophylls (UPS-UF) with a dose of 100 mg/kg. In the mice given either a low-molecular-weight UPS fucoidan or fucoidan source from Fucus vesiculosus (FV-UF), reperfusion was delayed at 55.0 ± 8.0 min with a higher reperfusion effective dose (RED) of 1 g/kg or at 63.3 ± 7.2 at RED of 200 mg/kg, respectively. In the control mice given t-PA of 15 mg/kg, reperfusion occurred at 24.8 ± 6.5 min after administration. In contrast, reperfusion was not observed in the occluded mice given heparin (P < 0.001) in the range of 60-1000 mg/kg. Minimal injection of fucoidan in addition to a given t-PA-enabled restoration of blood flow in the blocked artery without reocclusion at 17.2 ± 2.3 min postinjection (P < 0.002). In conclusion, algal fucoidan has both thrombolytic activity and a stimulatory effect on the thrombolytic activity of t-PA in a dose-dependent manner at an arterial thrombosis model.
authors.affiliation: Department of Biomedical Engineering and Radiology, School of Medicine, Catholic University of Daegu, Daegu, Korea.
authors.forename: Soon-Ki
authors.initials: SK
authors.lastname: Min
authors.forename: Sung-Mi
authors.initials: SM
authors.lastname: Han
authors.forename: Hong-Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Oh-Choon
authors.initials: OC
authors.lastname: Kwon
authors.forename: Sub
authors.initials: S
authors.lastname: Lee
authors.forename: Jong-Ki
authors.initials: JK
authors.lastname: Kim
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000187:drug effects* / Q000503:physiopathology; D002712:Chlorides / Q000494:pharmacology; D003328:Coronary Thrombosis / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005290:Ferric Compounds / Q000494:pharmacology; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D006493:Heparin / Q000627:therapeutic use; D007275:Injections, Intravenous; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000627:therapeutic use*; D015912:Thrombolytic Therapy / Q000379:methods; D010959:Tissue Plasminogen Activator / Q000627:therapeutic use*; D044648:Undaria / Q000737:chemistry
chemical_list: D002712:Chlorides; D005290:Ferric Compounds; D005343:Fibrinolytic Agents; D011134:Polysaccharides; D006493:Heparin; C007789:fucoidan; D010959:Tissue Plasminogen Activator; C024555:ferric chloride