title: Effect of low level laser therapy and zoledronate on the viability and ALP activity of Saos-2 cells.
abstract: A limited number of clinical studies indicate the supportive role of low level laser therapy (LLLT) on medical and/or surgical approaches carried out in treatment modalities for bisphosphonate related necrosis of jaws (BRONJ), the most common side effect of bisphosphonates used to inhibit bone resorption. The purpose of this study was to investigate the effects of LLLT on cell proliferation and alkaline phosphatase (ALP) activity of human osteoblast-like cells (Saos-2) treated with different doses of zoledronate, the most potent bisphosphonate. Saos-2 cells were treated with different concentrations of zoledronate and were irradiated with diode laser (wavelength 808 nm, 10 s, 0.25 or 0.50 W). Cell numbers and ALP activity of the cells were determined. LLLT mildly increased the proliferation rate or ALP activity, while zoledronate reduced both. When applied together, LLLT lessened the detrimental effects of zoledronate and improved cell function and/or proliferation. Based on the results of this study, it was concluded that LLLT has biostimulative effects on Saos-2 cells, even after treatment with zoledronate. LLLT may serve as a useful supportive method for BRONJ treatment through enhancement of healing by osteoblasts.
authors.affiliation: Hacettepe University, Fac. of Dentistry, Dept. of OMFS, Ankara, Turkey. cetinhilal@yahoo.com
authors.forename: Hilal
authors.initials: H
authors.lastname: Bayram
authors.forename: Halime
authors.initials: H
authors.lastname: Kenar
authors.forename: Ferda
authors.initials: F
authors.lastname: Taşar
authors.forename: Vasıf
authors.initials: V
authors.lastname: Hasırcı
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects / Q000528:radiation effects*; D050071:Bone Density Conservation Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002452:Cell Count; D018929:Cell Culture Techniques; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D048430:Cell Shape / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004164:Diphosphonates / Q000008:administration & dosage / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007093:Imidazoles / Q000008:administration & dosage / Q000627:therapeutic use*; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D028022:Low-Level Light Therapy / Q000379:methods*; D010006:Osteoblasts / Q000187:drug effects / Q000528:radiation effects*; D011829:Radiation Dosage; D000077211:Zoledronic Acid
chemical_list: D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D007093:Imidazoles; D000077211:Zoledronic Acid; D000469:Alkaline Phosphatase