title: [Protective effect of genistein on hypoxic injuries of osteoblasts cultivated in vitro].
abstract: OBJECTIVE
  To investigate the effect of genistein on osteoblast proliferation, cellular cycle, apoptosis and differentiation of osteoblasts cultivated under hypoxia conditions.
  
  
  METHODS
  Rat osteoblasts were isolated from calvarias by enzyme digestion and a hypoxic model was established by in a triple-gas incubator. Rat osteoblasts were grouped into the normoxic control group, the hypoxia control group and the hypoxia administration group which was subdivided into Ge-6 group, Ge-5 group and Ge-4 group, to which genistein was administered at doses of 1 x 10(-6), 1 x 10(-5), 1 x 10(-4) mol x L(-1). The cell survival rate, lactic dehydrogenase leakage rate, apoptosis and differentiation of osteoblasts were observed for each group at 3 h after hypoxia, and the gene expression of HIF-1alpha, Bcl-2, Caspase-3 was detected by Real time RT-PCR. Forty-eight hours after hypoxia, osteogenic differentiation markers including alkaline phosphatase activity and nodules were detected.
  
  
  RESULTS
  Compared with the hypoxia control group, the hypoxia administration group displays a significant increase in the survival rate and a decreased in LDH leakage rate, apoptosis rate and percentage of S + G2 phases. Besides, the mRNA level of HIF-1alpha and Bcl-2 were enhanced, the mRNA level of Caspase-3 was inhibited.
  
  
  CONCLUSIONS
  Genistein has an effect on protecting osteoblasts from hypoxia.
authors.affiliation: Institute of Orthopedics, Lanzhou General Hospital, Lanzhou 730050, China. jnhanqiu@163.com
authors.forename: Guiqiu
authors.initials: G
authors.lastname: Han
authors.forename: Baofeng
authors.initials: B
authors.lastname: Ge
authors.forename: Keming
authors.initials: K
authors.lastname: Chen
authors.forename: Huiping
authors.initials: H
authors.lastname: Ma
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019254:Genes, bcl-2; D019833:Genistein / Q000494:pharmacology*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D019833:Genistein; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase; D053148:Caspase 3