title: Experimental arthritis exacerbates Aggregatibacter actinomycetemcomitans-induced periodontitis in mice.
abstract: OBJECTIVE
  This study aimed to investigate whether chronic antigen-induced arthritis (AIA) influences infection-induced periodontitis (PD) in mice and whether PD modifies the clinical course of AIA. The contribution of anti-TNF-α therapy was also evaluated.
  
  
  METHODS
  The PD was induced in C57BL/6 mice by oral infection with Aggregatibacter actinomycetemcomitans. AIA was induced after infection. Anti-TNF-α and chlorhexidine therapies were used to investigate the role of TNF-α and oral infection on PD and AIA interaction. Maxillae, knee joints, lymph nodes and serum samples were used for histomorphometric, immunoenzymatic and/or real time-PCR analyses.
  
  
  RESULTS
  Antigen-induced arthritis exacerbated alveolar bone loss triggered by PD infection. In contrast, PD did not influence AIA in the evaluated time-points. PD exacerbation was associated with enhanced production of IFN-γ in maxillae and expression of the Th1 transcription factor tBET in submandibular lymph nodes. Increased serum levels of IL-6 and C-reactive protein were also detected. Anti-TNF-α and antiseptic therapies prevented the development and exacerbation of infectious-PD. Anti-TNF-α therapy also resulted in reduced expression of IFN-γ, TNF-α and IL-17 in maxillae.
  
  
  CONCLUSIONS
  Altogether, the current results indicate that the exacerbation of infection-induced PD by arthritis is associated with an alteration in lymphocyte polarization pattern and increased systemic immunoreactivity. This process was ameliorated by anti-TNF-α and antiseptic therapies.
authors.affiliation: Department of Oral Surgery and Pathology, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Celso Martins
authors.initials: CM
authors.lastname: Queiroz-Junior
authors.forename: Mila Fernandes Moreira
authors.initials: MF
authors.lastname: Madeira
authors.forename: Fernanda Matos
authors.initials: FM
authors.lastname: Coelho
authors.forename: Camila Ribeiro
authors.initials: CR
authors.lastname: de Oliveira
authors.forename: Luíza Castro Menezes
authors.initials: LC
authors.lastname: Cândido
authors.forename: Gustavo Pompermaier
authors.initials: GP
authors.lastname: Garlet
authors.forename: Mauro Martins
authors.initials: MM
authors.lastname: Teixeira
authors.forename: Daniele da Glória
authors.initials: Dda G
authors.lastname: de Souza
authors.forename: Tarcília Aparecida da
authors.initials: TA
authors.lastname: Silva
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000189:Actinobacillus Infections / Q000188:drug therapy / Q000382:microbiology*; D016976:Aggregatibacter actinomycetemcomitans / Q000502:physiology*; D016301:Alveolar Bone Loss / Q000276:immunology / Q000382:microbiology; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use; D001169:Arthritis, Experimental / Q000188:drug therapy / Q000276:immunology* / Q000382:microbiology; D002097:C-Reactive Protein / Q000032:analysis; D002710:Chlorhexidine / Q000627:therapeutic use; D024042:Collagen Type I / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000032:analysis; D020381:Interleukin-17 / Q000032:analysis; D015850:Interleukin-6 / Q000097:blood; D007527:Isoenzymes / Q000032:analysis; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008437:Maxilla / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010010:Osteoclasts / Q000473:pathology; D010518:Periodontitis / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology*; D020825:T-Box Domain Proteins / Q000032:analysis; D000071681:Tartrate-Resistant Acid Phosphatase; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors; D000098815:T-bet Transcription Factor
chemical_list: D000891:Anti-Infective Agents, Local; D024042:Collagen Type I; D007074:Immunoglobulin G; D020381:Interleukin-17; D015850:Interleukin-6; D007527:Isoenzymes; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D002097:C-Reactive Protein; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002710:Chlorhexidine