title: Effect of smoking on semen quality, FSH, testosterone level, and CAG repeat length in androgen receptor gene of infertile men in an Indian city.
abstract: This study was conducted as part of an epidemiological survey of 126 nonsmokers and 178 smokers, showing primary infertility residing around Kolkata region of Eastern India. Their lifestyle history including smoking habits along with semen and blood were collected. The study examined the association of cigarette smoking with the risk of infertility, by determining the semen quality, follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone levels, and androgen receptor (AR)-CAG repeat length in a group of smokers compared with a control group (non smokers). Based on conventional WHO criteria, lower sperm motility (P < 0.001) and increased sperm morphological defects (P < 0.0001) were associated with smoking habits. Binary logistic regression analysis for the effect of smoking status on sperm DNA integrity demonstrated significant positive correlation (p = 0.006). Serum FSH and LH levels were higher for smokers compared with non-smokers while the testosterone level decreased significantly with the increasing smoking habit. The mean length of CAG repeats in AR gene was significantly higher for smokers with low testosterone compared to non-smokers. The study suggested that smoking is associated with altered semen quality, endocrine hormonal status, and number of CAG repeats in the AR gene.
authors.affiliation: Institute of Reproductive Medicine, Kolkata, India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Mitra
authors.forename: Baidyanath
authors.initials: B
authors.lastname: Chakraborty
authors.forename: Dyutiman
authors.initials: D
authors.lastname: Mukhopadhay
authors.forename: Manisha
authors.initials: M
authors.lastname: Pal
authors.forename: Sanjit
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Samir
authors.initials: S
authors.lastname: Banerjee
authors.forename: Keya
authors.initials: K
authors.lastname: Chaudhuri
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016009:Chi-Square Distribution; D043373:Follicle Stimulating Hormone, Human / Q000097:blood*; D006801:Humans; D007194:India / Q000453:epidemiology; D007248:Infertility, Male / Q000097:blood / Q000453:epidemiology* / Q000208:ethnology / Q000235:genetics / Q000473:pathology; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D011944:Receptors, Androgen / Q000235:genetics*; D018570:Risk Assessment; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects* / Q000208:ethnology; D013081:Sperm Motility; D013094:Spermatozoa / Q000473:pathology*; D013739:Testosterone / Q000097:blood*; D018911:Trinucleotide Repeats; D014504:Urban Health / Q000208:ethnology
chemical_list: C410798:AR protein, human; D015415:Biomarkers; D043373:Follicle Stimulating Hormone, Human; D011944:Receptors, Androgen; D013739:Testosterone