title: Multifunctionality of indocyanine green-loaded biodegradable nanoparticles for enhanced optical imaging and hyperthermia intervention of cancer.
abstract: The aim of this study was to develop and characterize multifunctional biodegradable and biocompatible poly lactic-co-glycolic acid (PLGA) nanoparticles loaded with indocyanine green (ICG) as an optical-imaging contrast agent for cancer imaging and as a photothermal therapy agent for cancer treatment. PLGA-ICG nanoparticles (PIN) were synthesized with a particle diameter of 246±11 nm, a polydispersity index of 0.10±0.03, and ICG loading efficiency of 48.75±5.48%. PIN were optically characterized with peak excitation and emission at 765 and 810±5 nm, a fluorescence lifetime of 0.30±0.01 ns, and peak absorbance at 780 nm. The cytocompatibility study of PIN showed 85% cell viability till 1-mg/ml concentration of PIN. Successful cellular uptake of ligand conjugated PIN by prostate cancer cells (PC3) was also obtained. Both phantom-based and in vitro cell culture results demonstrated that PIN (1) have the great potential to induce local hyperthermia (i.e., temperature increase of 8 to 10°C) in tissue within 5 mm both in radius and in depth; (2) result in improved optical stability, excellent biocompatibility with healthy cells, and a great targeting capability; (3) have the ability to serve as an image contrast agent for deep-tissue imaging in diffuse optical tomography.
authors.affiliation: University of Texas, Department of Bioengineering, Joint Graduate Program between University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, Texas 76019, USA.
authors.forename: Ronak H
authors.initials: RH
authors.lastname: Patel
authors.forename: Aniket S
authors.initials: AS
authors.lastname: Wadajkar
authors.forename: Nimit L
authors.initials: NL
authors.lastname: Patel
authors.forename: Venkaiah C
authors.initials: VC
authors.lastname: Kavuri
authors.forename: Kytai T
authors.initials: KT
authors.lastname: Nguyen
authors.forename: Hanli
authors.initials: H
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry / Q000494:pharmacology*; D003952:Diagnostic Imaging; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D007208:Indocyanine Green / Q000737:chemistry / Q000494:pharmacology*; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D009369:Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000628:therapy*; D010316:Particle Size; D019047:Phantoms, Imaging; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013050:Spectrometry, Fluorescence; D013696:Temperature; D041622:Tomography, Optical / Q000379:methods
chemical_list: D003287:Contrast Media; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D007208:Indocyanine Green