title: Caveolin-1 interferes cell growth of lung cancer NCI-H446 cell through the interactions with phospho-ERK1/2, estrogen receptor and progestin receptor.
abstract: Caveolin-1 (CAV-1) either functions as a tumor suppressor gene or as an oncogene depending on the types of tumor cells and tumors. In current work, we investigated the influences of CAV-1 on the proliferation and cell cycle of small cell lung cancer (SCLC) cell NCI-H446, empty vector transfected NCI-H446 (NCI-H446-neo) and wild-type CAV-1 gene stably transfected NCI-H446 (NCI-H446-CAV-1) cells and explored the potential underlying mechanism. The colony formation capacity of NCI-H446-CAV-1 cell was 58.5% of that for NCI-H446 cell and 57.0% of that for NCI-H446-neo cell. CAV-1 inhibited the cell growth and cell cycle distribution of NCI-H446 cell in vitro. CAV-1 over-expression decreased the population of NCI-H446 cell at S phase and blocked NCI-H446 cell at G2/M phase without apparent effect on G1/G0 cell population. The level of phosphoryalted extracellular signal-regulated kinases (p-ERK1/2) instead of whole ERK1/2 in NCI-H446 cell was dramatically decreased following the stable expression of CAV-1. ERK1/2 phosphorylation might be critical for NCI-H446 cell growth. This work also revealed CAV-1 potentially regulated NCI-H446 growth in a hormone-dependant manner. Estrogen receptor (ER) and progestin receptor (PR) were significantly down-regulated in NCI-H446-CAV-1 cell comparing to NCI-H446 and NCI-H446-neo cells. Taken together, CAV-1 affected cell growth of lung cancer NCI-H446 cell through the interactions with p-ERK1/2, ER and PR.
authors.affiliation: Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, 9 West Lvshun Southern Road, Dalian 116044, PR China.
authors.forename: Ming-Zhong
authors.initials: MZ
authors.lastname: Sun
authors.forename: Zhuzhu
authors.initials: Z
authors.lastname: Guan
authors.forename: Shuqing
authors.initials: S
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Shujuan
authors.initials: S
authors.lastname: Shao
authors.forename: Dongmei
authors.initials: D
authors.lastname: Lin
mesh_terms: D051242:Caveolin 1 / Q000235:genetics*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010766:Phosphorylation; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D055752:Small Cell Lung Carcinoma / Q000473:pathology*; D014162:Transfection
chemical_list: D051242:Caveolin 1; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3