title: Effects of endothelin-1 on the cytoskeleton protein F-actin of human trabecular meshwork cells in vitro.
abstract: OBJECTIVE
  To observe the effect of endothelin-1 (ET-1) on the cytoskeleton protein F-actin of cultured human trabecular meshwork (HTM) cells.
  
  
  METHODS
  CULTURED HTM CELLS WERE RANDOMLY DIVIDED INTO FOUR GROUPS: control group, low-dose ET-1 (10(-9) mol/L) treatment group, middle-dose ET-1 (10(-8) mol/L) treatment group, and high-dose ET-1(10(-7) mol/L) treatment group. After treated with ET-1, the expression of cytoskeleton protein F-actin in trabecular meshwork was analyzed with Western-blot and the distribution of F-actin was detected with FITC-Phalloidin probe.
  
  
  RESULTS
  ET-1 dose-dependently and significantly increased F-actin in trabecular meshwork cells (P<0.05). The F-actin stress fiber and periphery actin fiber highly increased and manifested mild reorganization after treated with ET-1; and there were much more cell-to-cell and cell-to-extracellular matrix attachments formation in ET-1 treated HTM cells than that in the untreated HTM cells.
  
  
  CONCLUSIONS
  ET-1 promotes the expression of cytoske- leton protein F-actin and induced the trabecular meshwork actin cytoskeleton reorganization.
authors.affiliation: Department of Ophthalmology, Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiang
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Gui-Gang
authors.initials: GG
authors.lastname: Li
authors.forename: Rui-Lin
authors.initials: RL
authors.lastname: Wang
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Ling-Juan
authors.initials: LJ
authors.lastname: Xu
authors.forename: Lan
authors.initials: L
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang