title: Evaluation of the constitutive properties of native, tissue engineered, and degenerated articular cartilage.
abstract: BACKGROUND
  Conventional models to evaluate degenerated cartilage do not consider nonlinear permeability and proteoglycan viscous effects. Some models also utilize spring elements to represent the viscous effects of the fibers, thus application tothe modeling of nonuniform deformations such as those that occur in indentation tests. The purpose of this study was to assess the changes in the mechanical behavior of tissue engineered and degraded cartilage while addressing these shortcomings and limitations.
  
  
  METHODS
  An inverse finite element method was used to determine the material properties of native and tissue engineered cartilage from indentation test data. The engineered cartilage was evaluated 3 and 9 months after implantation into osteochondral defects in the trochlear groove of sheep stifles. The strain rate dependent responses of the engineered and native cartilage in unconfined compression were also determined for strain rates ranging from 0 to 20% s(-1). The material properties of bovine cartilage before and after proteoglycan depletion and collagen degradation were also compared using unconfined compression test data from the literature.
  
  
  RESULTS
  For a given strain, the stiffness of the engineered cartilage was approximately one tenth of that of the native cartilage both at 3 and 9 months. The model appeared to be able to predict the equilibrium and transient mechanical function of degenerated cartilage, and discerned the proteoglycan loss from collagen degradation.
  
  
  CONCLUSIONS
  The model can be used for high strain and dynamic analysis of cartilage, and may in the future allow the determination of the load bearing capability of engineered cartilage.
authors.affiliation: Department of Mechanical and Industrial Engineering, Ryerson University, 350 Victoria Street, Toronto, Ontario, Canada, M5B2K3.
authors.forename: A
authors.initials: A
authors.lastname: Seifzadeh
authors.forename: D C D
authors.initials: DC
authors.lastname: Oguamanam
authors.forename: M
authors.initials: M
authors.lastname: Papini
mesh_terms: D000818:Animals; D002356:Cartilage / Q000473:pathology; D002358:Cartilage, Articular / Q000473:pathology / Q000503:physiopathology*; D002417:Cattle; D019902:Chondrocytes / Q000378:metabolism; D002810:Chondroitinases and Chondroitin Lyases / Q000378:metabolism; D003094:Collagen / Q000737:chemistry; D019245:Compressive Strength; D020342:Finite Element Analysis; D008962:Models, Theoretical; D010003:Osteoarthritis / Q000473:pathology / Q000503:physiopathology; D010539:Permeability; D011509:Proteoglycans / Q000737:chemistry; D012756:Sheep; D013314:Stress, Mechanical; D013718:Tensile Strength; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D014783:Viscosity
chemical_list: D011509:Proteoglycans; D003094:Collagen; D002810:Chondroitinases and Chondroitin Lyases