title: Functional analysis of the putative tumor suppressor PTPRD in neuroblastoma cells.
abstract: The gene encoding PTPδ is mutated or downregulated in human cancers including neuroblastoma. Here, we functionally tested the tumor-suppressive potential of PTPδ in neuroblastoma cell lines by reconstitution of both short and long PTPδ isoforms. We did not observe any significant difference in colony forming ability between cells expressing wild-type or catalytically inactive PTPδ. Although endogenous PTPδ expression was very low in neuroblastoma cells, it was also low in mouse embryo adrenal glands, suggesting that PTPδ may have little developmental function in early adrenal neuroblasts. This study, therefore, questions the significance of PTPδ as a tumor suppressor protein in neuroblastoma.
authors.affiliation: Neural Development Unit, UCL Institute of Child Health, University College London, London, UK.
authors.forename: O
authors.initials: O
authors.lastname: Clark
authors.forename: F
authors.initials: F
authors.lastname: Schmidt
authors.forename: C H
authors.initials: CH
authors.lastname: Coles
authors.forename: V
authors.initials: V
authors.lastname: Tchetchelnitski
authors.forename: A W
authors.initials: AW
authors.lastname: Stoker
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology*; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2 / Q000502:physiology*; D025521:Tumor Suppressor Proteins / Q000502:physiology*
chemical_list: D025521:Tumor Suppressor Proteins; C090899:PTPRD protein, human; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2