title: Resonance Energy Transfer between protein and rhamnolipid capped ZnS quantum dots: application in in-gel staining of proteins.
abstract: The interaction of proteins with quantum dots is an interesting field of research. These interactions occur at the nanoscale. We have probed the interaction of Bovine Serum Albumin (BSA) and Candida rugosa lipase (CRL) with rhamnolipid capped ZnS (RhlZnSQDs) using absorption and fluorescence spectroscopy. Optical studies on mixtures of RhlZnSQDs and proteins resulted in Förster's Resonance Energy Transfer (FRET) from proteins to QDs. This phenomenon has been exploited to detect proteins in agarose gel electrophoresis. The activity of the CRL was unaffected on the addition of QDs as revealed by zymography.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai 600025, India.
authors.forename: Narayanan
authors.initials: N
authors.lastname: Janakiraman
authors.forename: Abhilash
authors.initials: A
authors.lastname: Mohan
authors.forename: Ashwin
authors.initials: A
authors.lastname: Kannan
authors.forename: Gautam
authors.initials: G
authors.lastname: Pennathur
mesh_terms: D000818:Animals; D002175:Candida / Q000201:enzymology; D002417:Cattle; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004735:Energy Transfer; D006017:Glycolipids / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D045663:Quantum Dots; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D013440:Sulfides / Q000378:metabolism*; D014466:Ultraviolet Rays; D017967:Zinc Compounds / Q000378:metabolism*
chemical_list: D006017:Glycolipids; D013440:Sulfides; D017967:Zinc Compounds; C418382:rhamnolipid; D012710:Serum Albumin, Bovine; D008049:Lipase; C031238:zinc sulfide