title: A new unusual delta11(12)-oleane triterpene and anti-complementary triterpenes from Prunella vulgaris spikes.
abstract: Anti-complementary activity-guided fractionation of the ethanolic extract of Prunella vulgaris spikes led to the isolation of a new, unusual delta11(12) triterpene, 3beta,13beta-dihydroxyolic-11-ene-28-oic acid (1), along with thirteen known triterpenes (2-14). The structure of the new compound was established by 1D and 2D NMR spectroscopic analysis. All the isolates were evaluated for their anti-complementary activity against the classical pathway (CP) and alternative pathway (AP). Eight triterpenes (1-8) showed anti-complementary activity against CP and AP, with CH50 and AP50 values of 0.15-0.37 mg/mL, and 0.29-0.53 mg/mL, respectively. Mechanism study using complement-depleted sera showed that oleanolic acid (2) acted selectively on C1q, C5, and C9 components, while 2alpha-hydroxy oleanolic acid (3) interacted with C1q, C3, C5 and C9, and 2alpha,3alpha-dihydroxyolic-12-ene-28-oic acid (4) blocked C1q, C2, C3, C5 and C9.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, PR China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Du
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cheng
authors.forename: Daofeng
authors.initials: D
authors.lastname: Chen
mesh_terms: D003165:Complement System Proteins / Q000737:chemistry*; D015394:Molecular Structure; D031347:Prunella / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D014315:Triterpenes; D003165:Complement System Proteins