title: The footprint of metabolism in the organization of mammalian genomes.
abstract: BACKGROUND
  At present five evolutionary hypotheses have been proposed to explain the great variability of the genomic GC content among and within genomes: the mutational bias, the biased gene conversion, the DNA breakpoints distribution, the thermal stability and the metabolic rate. Several studies carried out on bacteria and teleostean fish pointed towards the critical role played by the environment on the metabolic rate in shaping the base composition of genomes. In mammals the debate is still open, and evidences have been produced in favor of each evolutionary hypothesis. Human genes were assigned to three large functional categories (as well as to the corresponding functional classes) according to the KOG database: (i) information storage and processing, (ii) cellular processes and signaling, and (iii) metabolism. The classification was extended to the organisms so far analyzed performing a reciprocal Blastp and selecting the best reciprocal hit. The base composition was calculated for each sequence of the whole CDS dataset.
  
  
  RESULTS
  The GC3 level of the above functional categories was increasing from (i) to (iii). This specific compositional pattern was found, as footprint, in all mammalian genomes, but not in frog and lizard ones. Comparative analysis of human versus both frog and lizard functional categories showed that genes involved in the metabolic processes underwent the highest GC3 increment. Analyzing the KOG functional classes of genes, again a well defined intra-genomic pattern was found in all mammals. Not only genes of metabolic pathways, but also genes involved in chromatin structure and dynamics, transcription, signal transduction mechanisms and cytoskeleton, showed an average GC3 level higher than that of the whole genome. In the case of the human genome, the genes of the aforementioned functional categories showed a high probability to be associated with the chromosomal bands.
  
  
  CONCLUSIONS
  In the light of different evolutionary hypotheses proposed so far, and contributing with different potential to the genome compositional heterogeneity of mammalian genomes, the one based on the metabolic rate seems to play not a minor role. Keeping in mind similar results reported in bacteria and in teleosts, the specific compositional patterns observed in mammals highlight metabolic rate as unifying factor that fits over a wide range of living organisms.
authors.affiliation: Genome Evolution and Organization - Department Animal Physiology and Evolution, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Berná
authors.forename: Ankita
authors.initials: A
authors.lastname: Chaurasia
authors.forename: Claudia
authors.initials: C
authors.lastname: Angelini
authors.forename: Concetta
authors.initials: C
authors.lastname: Federico
authors.forename: Salvatore
authors.initials: S
authors.lastname: Saccone
authors.forename: Giuseppe
authors.initials: G
authors.lastname: D'Onofrio
mesh_terms: D000818:Animals; D001001:Anura / Q000235:genetics / Q000378:metabolism; D001482:Base Composition / Q000235:genetics; D033481:Chromosomes, Mammalian / Q000235:genetics; D030541:Databases, Genetic; D019143:Evolution, Molecular; D016678:Genome / Q000235:genetics*; D006801:Humans; D008116:Lizards / Q000235:genetics / Q000378:metabolism; D008322:Mammals / Q000235:genetics*; D055432:Metabolomics