title: Cold atmospheric air plasma sterilization against spores and other microorganisms of clinical interest.
abstract: Physical cold atmospheric surface microdischarge (SMD) plasma operating in ambient air has promising properties for the sterilization of sensitive medical devices where conventional methods are not applicable. Furthermore, SMD plasma could revolutionize the field of disinfection at health care facilities. The antimicrobial effects on Gram-negative and Gram-positive bacteria of clinical relevance, as well as the fungus Candida albicans, were tested. Thirty seconds of plasma treatment led to a 4 to 6 log(10) CFU reduction on agar plates. C. albicans was the hardest to inactivate. The sterilizing effect on standard bioindicators (bacterial endospores) was evaluated on dry test specimens that were wrapped in Tyvek coupons. The experimental D(23)(°)(C) values for Bacillus subtilis, Bacillus pumilus, Bacillus atrophaeus, and Geobacillus stearothermophilus were determined as 0.3 min, 0.5 min, 0.6 min, and 0.9 min, respectively. These decimal reduction times (D values) are distinctly lower than D values obtained with other reference methods. Importantly, the high inactivation rate was independent of the material of the test specimen. Possible inactivation mechanisms for relevant microorganisms are briefly discussed, emphasizing the important role of neutral reactive plasma species and pointing to recent diagnostic methods that will contribute to a better understanding of the strong biocidal effect of SMD air plasma.
authors.affiliation: Max Planck Institute for Extraterrestrial Physics, Garching, Germany. klaempfl@mpe.mpg.de
authors.forename: Tobias G
authors.initials: TG
authors.lastname: Klämpfl
authors.forename: Georg
authors.initials: G
authors.lastname: Isbary
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Shimizu
authors.forename: Yang-Fang
authors.initials: YF
authors.lastname: Li
authors.forename: Julia L
authors.initials: JL
authors.lastname: Zimmermann
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Stolz
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schlegel
authors.forename: Gregor E
authors.initials: GE
authors.lastname: Morfill
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Schmidt
mesh_terms: D001272:Atmosphere / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D015169:Colony Count, Microbial; D058626:Plasma Gases / Q000032:analysis / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D013171:Spores, Bacterial / Q000187:drug effects*; D013242:Sterilization / Q000379:methods*
chemical_list: D058626:Plasma Gases