title: Thymoquinone, the Nigella sativa bioactive compound, prevents circulatory oxidative stress caused by 1,2-dimethylhydrazine in erythrocyte during colon postinitiation carcinogenesis.
abstract: We have performed this study to investigate the modulatory effect of thymoquinone (TQ), the Nigella sativa active compound, on erythrocyte lipid peroxidation and antioxidant status during 1,2-dimethylhydrazine- (DMH-) induced colon carcinogenesis after initiation in male Wistar rats. Rats exposed to DMH showed an increase of malondialdehyde and conjugated diene levels, and an augmentation of enzyme activities like catalase, glutathione peroxidase, and superoxide dismutase activities was also noted. The TQ pretreatment restored the parameters cited above to near-normal values. However, the posttreatment shows an activity similar as that presented by DMH. Therefore, our investigation revealed that TQ was a useful compound preventing DMH-induced erythrocyte damages.
authors.affiliation: Research Unit of Cancer Biology and Genetics, Hematologic, and Autoimmune Diseases, Faculty of Pharmacy of Monastir, University of Monastir, 5000 Monastir, Tunisia. jrahhanene@yahoo.fr
authors.forename: Hanene Jrah
authors.initials: HJ
authors.lastname: Harzallah
authors.forename: Rahma
authors.initials: R
authors.lastname: Grayaa
authors.forename: Wafa
authors.initials: W
authors.lastname: Kharoubi
authors.forename: Aya
authors.initials: A
authors.lastname: Maaloul
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hammami
authors.forename: Touhami
authors.initials: T
authors.lastname: Mahjoub
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000037:antagonists & inhibitors* / Q000633:toxicity; D000818:Animals; D016227:Benzoquinones / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology*; D003110:Colonic Neoplasms / Q000097:blood* / Q000473:pathology*; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D031881:Nigella sativa / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016227:Benzoquinones; D006454:Hemoglobins; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D019813:1,2-Dimethylhydrazine; C003466:thymoquinone