title: PhnY and PhnZ comprise a new oxidative pathway for enzymatic cleavage of a carbon-phosphorus bond.
abstract: The sequential activities of PhnY, an α-ketoglutarate/Fe(II)-dependent dioxygenase, and PhnZ, a Fe(II)-dependent enzyme of the histidine-aspartate motif hydrolase family, cleave the carbon-phosphorus bond of the organophosphonate natural product 2-aminoethylphosphonic acid. PhnY adds a hydroxyl group to the α-carbon, yielding 2-amino-1-hydroxyethylphosphonic acid, which is oxidatively converted by PhnZ to inorganic phosphate and glycine. The PhnZ reaction represents a new enzyme mechanism for metabolic cleavage of a carbon-phosphorus bond.
authors.affiliation: Department of Chemistry, Queen's University, Kingston, Ontario, Canada.
authors.forename: Fern R
authors.initials: FR
authors.lastname: McSorley
authors.forename: Peter B
authors.initials: PB
authors.lastname: Wyatt
authors.forename: Asuncion
authors.initials: A
authors.lastname: Martinez
authors.forename: Edward F
authors.initials: EF
authors.lastname: DeLong
authors.forename: Bjarne
authors.initials: B
authors.lastname: Hove-Jensen
authors.forename: David L
authors.initials: DL
authors.lastname: Zechel