title: Successful circumferential free tracheal transplantation in a large animal model.
abstract: BACKGROUND
  Long segment tracheobronchial stenoses are associated with high morbi-mortality rates and difficult treatment. Transplantation hasn't proved to be useful yet. Currently, the successful results achieved in small animal models couldn't be satisfactorily accomplished or extrapolated in large mammals. We aimed to evaluate the viability of orthotopic tracheal autoimplantation in an ovine model.
  
  
  METHODS
  All animals underwent tracheal transplantation of 4 cm (5-7 rings) of the cervical trachea and were divided randomly in two groups: isolated autoimplantation (Group A/6) and autoimplantation with omental wrapping (Group B/6). Clinical follow up and weekly bronchoscopical examinations were performed. The grafts were macroscopically, histologically, and bacteriologically analyzed.
  
  
  RESULTS
  In group A, four animals achieved their planed survival and were sacrificed up to 60 days after transplantation with viable grafts. In group B, only two sheep had successful results. Graft failure with infection, necrosis and severe stenosis was observed in the rest of the animals from both groups. Pseudomonas aeruginose was isolated in all cases. The main complication of the omental pedicle was vascular congestion and peritracheal hemorrhage.
  
  
  CONCLUSIONS
  Contrary to the data reported to date, we found that tracheal transplantation is viable in a large mammal like the sheep. The main complication observed in this animal model was graft infection. The use of an omental pedicle with the technique applied worsened the grafts survival. The encouraging results obtained in this investigation justify further research in order to manage graft infection, leading us to establish a suitable large animal model for allotransplantation.
authors.affiliation: Cardiovascular and Thoracic Surgery Division, Favaloro Foundation University Hospital,   Buenos Aires,   Argentina.
authors.forename: Alejandro M
authors.initials: AM
authors.lastname: Bertolotti
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Alvarez
authors.forename: Sebastián
authors.initials: S
authors.lastname: Defranchi
authors.forename: Mónica
authors.initials: M
authors.lastname: Alvarez
authors.forename: Rubén P
authors.initials: RP
authors.lastname: Laguens
authors.forename: Roberto R
authors.initials: RR
authors.lastname: Favaloro
mesh_terms: D000818:Animals; D001999:Bronchoscopy; D008297:Male; D023421:Models, Animal; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012756:Sheep; D014132:Trachea / Q000473:pathology / Q000637:transplantation*; D014182:Transplantation, Autologous