title: Directed assembly of DNA-functionalized gold nanoparticles using pyrrole-imidazole polyamides.
abstract: Traditional methods for the construction of nanoparticle arrays and lattices exploit Watson-Crick base pairing of single-stranded DNA sequences as a proxy for self-assembly. Although this approach has been utilized in a variety of applications in nanoassembly, diagnostics, and biomedicine, the diversity of this recognition lexicon could be considerably increased by developing strategies that recognize the base-pairing landscape of double-stranded DNA (dsDNA) sequences. Herein we describe the first report of programmed gold nanoparticle (GNP) aggregation directed by the recognition of dsDNA sequences using pyrrole-imidazole polyamide-GNP (PA-GNP) conjugates. We demonstrate the reversibility and selectivity of this strategy for forming GNP aggregates in the presence of fully matched dsDNA sequences relative to dsDNA sequences containing one- and two-base-pair mismatches.
authors.affiliation: Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, The University of Strathclyde, Glasgow, United Kingdom.
authors.forename: Zeljka
authors.initials: Z
authors.lastname: Krpetić
authors.forename: Ishwar
authors.initials: I
authors.lastname: Singh
authors.forename: Wu
authors.initials: W
authors.lastname: Su
authors.forename: Luca
authors.initials: L
authors.lastname: Guerrini
authors.forename: Karen
authors.initials: K
authors.lastname: Faulds
authors.forename: Glenn A
authors.initials: GA
authors.lastname: Burley
authors.forename: Duncan
authors.initials: D
authors.lastname: Graham
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009757:Nylons / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D007093:Imidazoles; D009757:Nylons; D011758:Pyrroles; D006046:Gold; D004247:DNA