title: Evaluation of the nitrate reductase assay for the rapid detection of resistance to first-line medications in Mycobacterium tuberculosis strains isolated from patients in a general hospital.
abstract: We compared the nitrate reductase assay with the proportion method, which is considered the gold standard, in 57 Mycobacterium tuberculosis strains isolated from patients treated at the Federal University of Minas Gerais Hospital das Clínicas, located in the city of Belo Horizonte, Brazil. For rifampin and isoniazid, the sensitivity, specificity, and accuracy of the nitrate reductase assay were all 100%, whereas they were 100%, 88.9%, and 66.7%, respectively, for streptomycin and 98.0%, 100%, and 98.2%, respectively, for ethambutol. The mean time to results was ten days. In the study sample, the nitrate reductase assay proved highly accurate and showed excellent concordance with the gold standard.
authors.affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. ffafilardi@gmail.com
authors.forename: Maria de Fátima Filardi Oliveira
authors.initials: Mde F
authors.lastname: Mansur
authors.forename: Wânia da Silva
authors.initials: Wda S
authors.lastname: Carvalho
authors.forename: Raquel Bandeira da
authors.initials: RB
authors.lastname: Silva
authors.forename: Rodrigo Gonçalves Cata
authors.initials: RG
authors.lastname: Preta
authors.forename: Lucas Almeida
authors.initials: LA
authors.lastname: Fernandes Junior
authors.forename: Silvana Spíndola de
authors.initials: SS
authors.lastname: Miranda
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D006769:Hospitals, General; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000201:enzymology*; D050901:Nitrate Reductase / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D000995:Antitubercular Agents; D015415:Biomarkers; D050901:Nitrate Reductase