title: [Phamacokinetic study of bergapten in rats plasma by LC-MS/MS].
abstract: OBJECTIVE
  To determine bergapten's concentration in plasma and observe its pharmacokinetics in rats using a combined LC-MS/MS analytical method.
  
  
  METHODS
  Blood samples were separated on a Hypersil ODS column (4.6 mm x 250 mm, 5 mm) at a temperature of 30 degrees C, and mobile phase consisted of water and methanol (22.5: 77.5) at a flow rate of 0.8 mL x min(-1).
  
  
  RESULTS
  The methodological study showed a good linear relationship of 8.12-162.4 g x L(-1) (r = 0.9999). The inner and inter-days relative standard deviations were both less than 10% , indicating legitimate precise and accuracy to the requirement of biological sample analysis.
  
  
  CONCLUSIONS
  The method is suitable for in vivo quantitative analysis for bergapten due to its accuracy, sensitivity and specificity. The pharmacokinetic process in rats forms a two-compartment model with first-order absorption.
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Ma
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Fangdi
authors.initials: F
authors.lastname: Hu
authors.forename: Yikai
authors.initials: Y
authors.lastname: Shi
authors.forename: Yingyan
authors.initials: Y
authors.lastname: Bi
authors.forename: Shilan
authors.initials: S
authors.lastname: Feng
mesh_terms: D000078223:5-Methoxypsoralen; D000818:Animals; D002853:Chromatography, Liquid; D008297:Male; D008730:Methoxsalen / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D000078223:5-Methoxypsoralen; D008730:Methoxsalen