title: Conjunctival and corneal tolerability assessment of ocular naltrexone niosomes and their ingredients on the hen's egg chorioallantoic membrane and excised bovine cornea models.
abstract: This study aimed at combining the hen's egg test-chorioallantoic membrane (HET-CAM), bovine corneal opacity and permeability (BCOP) test and histological examination of excised corneas to evaluate the conjunctival and corneal toxicity of niosomes and their ingredients. Various surfactant/lipid combinations and concentrations (1-10%, w/v) were investigated for the ocular delivery of an ambitious drug (naltrexone hydrochloride) for treatment of diabetic keratopathy. Four niosomal formulations were investigated and found to be non irritant to the 10 days old HET-CAMs (an acceptable conjunctival model). Only one of the tested ingredients (sodium cholate - CH) showed moderate irritation, however such an effect was diminished when incorporated into niosomes. Corneal opacity and fluorescein permeability scores for the test substances correlated well with the HET-CAM test results. Corneal erosion and stromal thickness were found to be in agreement with the HET-CAM and BCOP results, which discriminated well between moderately and mildly irritant test substances. Corneal histological examination revealed toxicity signs included epithelial erosion, stromal condensation and stromal vacuolisation, which allowed better discrimination between strong and moderate irritants. It is concluded that the prepared niosomes possess good ocular tolerability and minimal ocular tissue irritation. They can be further investigated as ocular delivery systems using appropriate animal models.
authors.affiliation: School of Pharmacy, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand. h.abdelkader@pharm.miniauniv.edu.eg
authors.forename: Hamdy
authors.initials: H
authors.lastname: Abdelkader
authors.forename: Sayed
authors.initials: S
authors.lastname: Ismail
authors.forename: Amal
authors.initials: A
authors.lastname: Hussein
authors.forename: Zimei
authors.initials: Z
authors.lastname: Wu
authors.forename: Raida
authors.initials: R
authors.lastname: Al-Kassas
authors.forename: Raid G
authors.initials: RG
authors.lastname: Alany
mesh_terms: D060433:Administration, Ophthalmic; D000818:Animals; D002417:Cattle; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000187:drug effects; D003228:Conjunctiva / Q000187:drug effects; D003315:Cornea / Q000187:drug effects / Q000473:pathology; D003318:Corneal Opacity / Q000139:chemically induced / Q000473:pathology; D005260:Female; D066298:In Vitro Techniques; D007509:Irritants / Q000633:toxicity*; D008081:Liposomes; D009271:Naltrexone / Q000633:toxicity*; D009292:Narcotic Antagonists / Q000633:toxicity*
chemical_list: D007509:Irritants; D008081:Liposomes; D009292:Narcotic Antagonists; D009271:Naltrexone