title: Desulfatitalea tepidiphila gen. nov., sp. nov., a sulfate-reducing bacterium isolated from tidal flat sediment.
abstract: A novel sulfate-reducing bacterium, strain S28bF(T), was isolated from tidal flat sediment from Tokyo Bay, Japan. Cells of strain S28bF(T) were rod-shaped (0.5-0.6×1.7-3.8 µm), motile and Gram-stain-negative. For growth, the optimum pH was pH 6.8-7.3 and the optimum temperature was 34-42 °C. Strain S28bF(T) used sulfate and thiosulfate as electron acceptors, but not nitrate. The G+C content of the genomic DNA was 56.6 mol%. The fatty acid profile of strain S28bF(T) was characterized by the presence of anteiso-C(15 : 0) and C(16 : 0) as the major components. Phylogenetic analyses based on genes for 16S rRNA, the alpha subunit of dissimilatory sulfite reductase (dsrA) and adenosine-5'-phosphosulfate reductase (aprA) revealed that the isolated strain belonged to the class Deltaproteobacteria. Its closest relative was Desulfosarcina cetonica DSM 7267(T) with a 16S rRNA gene sequence similarity of 93.3 %. Two other strains, S28OL1 and S28OL2 were also isolated from the same sediment. These strains were closely related to S28bF(T) with 16S rRNA gene sequence similarities of 99 %, and the same physiological characteristics were shared with strain S28bF(T). On the basis of phylogenetic and phenotypic characterization, a novel species in a new genus, Desulfatitalea tepidiphila gen. nov., sp. nov., is proposed to accommodate the strains obtained in this study. The type strain is S28bF(T) ( = NBRC 107166(T) = DSM 23472(T)).
authors.affiliation: Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Higashioka
authors.affiliation: Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan.
authors.forename: Hisaya
authors.initials: H
authors.lastname: Kojima
authors.affiliation: Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido 060-0819, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Fukui
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D020564:Deltaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D016999:Sulfur-Reducing Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S