title: [Inhibitory effect and mechanism of procyanidin from vaccinium on isoprenaline-induced myocardial fibrosis in rats].
abstract: OBJECTIVE
  To detect the effect and mechanism of procyanidin foom vaccinium (PC) on myocardial fibrosis in rats.
  
  
  METHODS
  The myocardial fibrosis model in rats was built by subcutaneous injection of 5 mg x kg(-1) x d(-1) of isoprenaline (Iso) for 7 days in vivo while intragastrically administrating PC 100, 200 and 400 mg x kg(-1) x d(-1) for 14 days. Following the model was established, myocardial indexes (heart weight/body weight, HW/BW and left ventricalar weight/body weight, LVW/BW) were measured. Besides, angiotensin II and I , III collagen quantification levels in blood serum were determined respectively by ELISA. The change in the content of nitric oxide (NO) in blood serum was determined with colorimetry. The content of malondialdehyde (MDA) in left ventricle was assayed with spectrophotometry. The contents of lactate dehydrogenase (LDH) and creatine kinase (CK) in blood serum were detected with automatic biochemistry analyzer.
  
  
  RESULTS
  Compared with the control group, the myocardial indexes, the contents of I , III collagen quantification, angiotensin II in blood serum and malondialdehyde in left ventricle were markedly increased and the content of nitric oxide in blood serum was decreased, the contents of lactate dehydrogenase and creatine kinase in blood serum were markedly increased in Iso model group (P<0.05 or P<0.01). Compared with the model group, the myocardial indexes were decreased, the contents of I , III collagen quantification, angiotensin II in blood serum were reduced in PC 200 and 400 mg x kg(-1) x d(-1) groups (P<0.05 or P<0.01). The content of nitric oxide in blood serum was increased, the content of malondialdehyde in left ventricle was markedly decreased, the contents of lactate dehydrogenase and creatine kinase in blood serum were markedly decreased in PC three groups (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  PC could inhibit collagen synthesis by decreasing angiotensin II level and increasing the content of nitric oxide and antioxidation, and thereby inhibiting myocardial fibrosis and protect myocardium in rats.
authors.affiliation: Department of Pharmacology, Jilin Medical College of Basic Medicine, Jilin 132013, China. fanhongyan1975@yahoo.com.cn
authors.forename: Hongyan
authors.initials: H
authors.lastname: Fan
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Wang
authors.forename: Kuang
authors.initials: K
authors.lastname: Ren
authors.forename: Raosheng
authors.initials: R
authors.lastname: Gu
authors.forename: Nan
authors.initials: N
authors.lastname: Shen
authors.forename: Ying
authors.initials: Y
authors.lastname: An
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhong
mesh_terms: D000809:Angiotensins / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D044946:Biflavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D002392:Catechin / Q000008:administration & dosage / Q000494:pharmacology*; D004719:Endomyocardial Fibrosis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D005260:Female; D007545:Isoproterenol / Q000009:adverse effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000097:blood; D044945:Proanthocyanidins / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D029796:Vaccinium / Q000737:chemistry*; D020257:Ventricular Remodeling / Q000187:drug effects
chemical_list: D000809:Angiotensins; D000975:Antioxidants; D044946:Biflavonoids; D044945:Proanthocyanidins; D009569:Nitric Oxide; C017674:procyanidin; D008315:Malondialdehyde; D002392:Catechin; D007545:Isoproterenol