title: Protection and antibody response induced by intramuscular DNA vaccine encoding for viral haemorrhagic septicaemia virus (VHSV) G glycoprotein in turbot (Scophthalmus maximus).
abstract: Turbot (Scophthalmus maximus) is a high-value farmed marine flatfish with growing demand and production levels in Europe susceptible to turbot-specific viral haemorrhagic septicaemia virus (VHSV) strains. To evaluate the possibility of controlling the outbreaks of this infectious disease by means of DNA vaccination, the gpG of a VHSV isolated from farmed turbot (VHSV(860)) was cloned into an expression plasmid containing the human cytomegalovirus (CMV) promoter (pMCV1.4-G(860)). In our experimental conditions, DNA immunised turbots were more than 85% protected against VHSV(860) lethal challenge and showed both VHSV-gpG specific and neutralizing antibodies. To our knowledge this is the first report showing the efficacy of turbot genetic immunisation against a VHSV. Work is in progress to determine the contribution of innate and adaptive immunity to the protective response elicited by the immunization.
authors.affiliation: Instituto de Investigaciones Marinas (IIM), CSIC, Vigo 36208, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Pereiro
authors.forename: A
authors.initials: A
authors.lastname: Martinez-Lopez
authors.forename: A
authors.initials: A
authors.lastname: Falco
authors.forename: S
authors.initials: S
authors.lastname: Dios
authors.forename: A
authors.initials: A
authors.lastname: Figueras
authors.forename: J M
authors.initials: JM
authors.lastname: Coll
authors.forename: B
authors.initials: B
authors.lastname: Novoa
authors.forename: A
authors.initials: A
authors.lastname: Estepa
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology*; D005412:Flatfishes / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D031941:Hemorrhagic Septicemia, Viral / Q000276:immunology*; D007273:Injections, Intramuscular; D029141:Novirhabdovirus / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D019444:Vaccines, DNA; D014760:Viral Fusion Proteins