title: Preparation and in vitro characterization of dexamethasone-loaded poly(D,L-lactic acid) microspheres embedded in poly(ethylene glycol)-poly({varepsilon}-caprolactone)-poly(ethylene glycol) hydrogel for orthopedic tissue engineering.
abstract: The corium is decreased to about half of its thickness in skin defects and wrinkles due to gravity and environment. In this study, dexamethasone/poly(d,l-lactic acid) (Mn = 160,000) microspheres were incorporated into poly(ethylene glycol)-poly(ε-caprolactone)-poly(ethylene glycol) (Mn = 3300) hydrogel to prepare an injectable hydrogel composite. The composite was designed to increase the thickness of the corium. Dexamethasone/poly(d,l-lactic acid) microspheres were prepared by oil-in-water emulsion/solvent evaporation technique. The properties of microspheres were investigated by size distribution measurement, scanning electron microscope and x-ray diffraction. Drug loading, encapsulation efficiency, and drug delivery behavior of microspheres were also studied in detail. Cell adhesion of microspheres was investigated by NIH3T3 cell in vitro. The properties of hydrogel composite were investigated by scanning electron microscope, rheological measurements and methyl thiazolyl tetrazolium assay. Drug release from composite was determined by HPLC-UV analysis. These results suggested that poly(d,l-lactic acid) microspheres encapsulating dexamethasone embedded in poly(ethylene glycol)-poly(ε-caprolactone)-poly(ethylene glycol) hydrogel might have prospective application in orthopedic tissue engineering field.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
authors.forename: Min
authors.initials: M
authors.lastname: Fan
authors.forename: QingFa
authors.initials: Q
authors.lastname: Guo
authors.forename: JingCong
authors.initials: J
authors.lastname: Luo
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
authors.forename: XiaoHai
authors.initials: X
authors.lastname: Tang
authors.forename: ZhiYong
authors.initials: Z
authors.lastname: Qian
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D003907:Dexamethasone / Q000008:administration & dosage*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D008863:Microspheres; D041681:NIH 3T3 Cells; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D023822:Tissue Engineering
chemical_list: D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; C502908:poly(ethylene glycol)-poly(caprolactone)-poly(ethylene glycol); D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); D003907:Dexamethasone