title: Nonenzymatic ligation of DNA with a reversible step and a final linkage that can be used in PCR.
abstract: Nonenzymatic DNA ligation chemistries containing a reversible step allow thermodynamic control of product formation, but they are not necessarily compatible with polymerase enzymes. We report a ligation system that uses commercially available reagents, includes a reversible step, and results in a linkage that can function as a template for PCR amplification with accurate sequence transfer.
authors.affiliation: School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.
authors.forename: Aaron E
authors.initials: AE
authors.lastname: Engelhart
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Cafferty
authors.forename: C Denise
authors.initials: CD
authors.lastname: Okafor
authors.forename: Michael C
authors.initials: MC
authors.lastname: Chen
authors.forename: Loren Dean
authors.initials: LD
authors.lastname: Williams
authors.forename: David G
authors.initials: DG
authors.lastname: Lynn
authors.forename: Nicholas V
authors.initials: NV
authors.lastname: Hud
mesh_terms: D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism; D009025:Morpholines / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D015342:DNA Probes; D009025:Morpholines; D004247:DNA; D004259:DNA-Directed DNA Polymerase