title: Formulation Strategies, Characterization, and In Vitro Evaluation of Lecithin-Based Nanoparticles for siRNA Delivery.
abstract: The aim of the present work was to take advantage of lecithin's biocompatibility along with its physicochemical properties for the preparation of lecithin-based nanocarriers for small interfering RNA (siRNA) delivery. Water lecithin dispersions were prepared in different conditions, loaded with siRNA at different N/P ratios, and evaluated for loading capacity. The most appropriate ones were then assayed for cytotoxicity and characterized in terms of particle size distribution, zeta potential, and morphology. Results demonstrated that formulations prepared at pH 5.0 and 7.0 were able to load siRNA at broad N/P ratios, and cellular uptake assays showed an efficient delivery of oligos in MCF-7 human breast cancer cells; fluorescent-labeled dsRNA mainly located next to its target, near the nucleus of the cells. No signs of toxicity were observed for broad compositions of lecithin. The physicochemical characterization of the siRNA-loaded dispersions exhibited particles of nanometric sizes and pH-dependant shapes, which make them suitable for ex vivo and in vivo further evaluation.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Junín 954 1113AAD Buenos Aires, Argentina.
authors.forename: Sebastián Ezequiel
authors.initials: SE
authors.lastname: Pérez
authors.forename: Yamila
authors.initials: Y
authors.lastname: Gándola
authors.forename: Adriana Mónica
authors.initials: AM
authors.lastname: Carlucci
authors.forename: Lorena
authors.initials: L
authors.lastname: González
authors.forename: Daniel
authors.initials: D
authors.lastname: Turyn
authors.forename: Carlos
authors.initials: C
authors.lastname: Bregni