title: Protein arraying by cell-free expression and diffusion across a fluid-filled gap.
abstract: An improved system for cell-free expression of protein arrays based on DNA arrays is presented. Our technology uses an array of DNA constructs for cell-free expression, which acts as a template instructing the generation of the corresponding protein array. Proteins are expressed locally from these templates by a cell-free transcription and translation system, and are immobilised on a separate capture surface overlayed on the DNA array. By simplifying the setup to allow protein diffusion between the slides across a gap filled with the cell-free system, we have markedly improved the evenness of the resulting protein microarrays.
authors.affiliation: Protein Technology Group, Babraham Bioscience Technologies Ltd, Cambridge CB22 3AT, United Kingdom.
authors.forename: Oda
authors.initials: O
authors.lastname: Stoevesandt
mesh_terms: D002474:Cell-Free System; D004247:DNA / Q000378:metabolism; D004058:Diffusion; D008567:Membranes, Artificial; D044085:Microfluidics / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000378:metabolism
chemical_list: D008567:Membranes, Artificial; D011506:Proteins; D004247:DNA