title: Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale.
abstract: A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.
authors.affiliation: F. Hoffmann-La Roche Ltd., 4070 Basel, Switzerland.
authors.forename: Steven P
authors.initials: SP
authors.lastname: Hanlon
authors.forename: Andrea
authors.initials: A
authors.lastname: Camattari
authors.forename: Sandra
authors.initials: S
authors.lastname: Abad
authors.forename: Anton
authors.initials: A
authors.lastname: Glieder
authors.forename: Matthias
authors.initials: M
authors.lastname: Kittelmann
authors.forename: Stephan
authors.initials: S
authors.lastname: Lütz
authors.forename: Beat
authors.initials: B
authors.lastname: Wirz
authors.forename: Margit
authors.initials: M
authors.lastname: Winkler
mesh_terms: D004798:Enzymes; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism*; D005415:Flavins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000378:metabolism; D020912:Moclobemide / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D004798:Enzymes; D005415:Flavins; D006899:Mixed Function Oxygenases; D020912:Moclobemide