title: A resource for the conditional ablation of microRNAs in the mouse.
abstract: The importance of miRNAs during development and disease processes is well established. However, most studies have been done in cells or with patient tissues, and therefore the physiological roles of miRNAs are not well understood. To unravel in vivo functions of miRNAs, we have generated conditional, reporter-tagged knockout-first mice for numerous evolutionarily conserved miRNAs. Here, we report the generation of 162 miRNA targeting vectors, 64 targeted ES cell lines, and 46 germline-transmitted miRNA knockout mice. In vivo lacZ reporter analysis in 18 lines revealed highly tissue-specific expression patterns and their miRNA expression profiling matched closely with published expression data. Most miRNA knockout mice tested were viable, supporting a mechanism by which miRNAs act redundantly with other miRNAs or other pathways. These data and collection of resources will be of value for the in vivo dissection of miRNA functions in mouse models.
authors.affiliation: UCSF Diabetes Center, UCSF, San Francisco, CA 94143, USA.
authors.forename: Chong Yon
authors.initials: CY
authors.lastname: Park
authors.forename: Lukas T
authors.initials: LT
authors.lastname: Jeker
authors.forename: Karen
authors.initials: K
authors.lastname: Carver-Moore
authors.forename: Alyssia
authors.initials: A
authors.lastname: Oh
authors.forename: Huey Jiin
authors.initials: HJ
authors.lastname: Liu
authors.forename: Rachel
authors.initials: R
authors.lastname: Cameron
authors.forename: Hunter
authors.initials: H
authors.lastname: Richards
authors.forename: Zhongmei
authors.initials: Z
authors.lastname: Li
authors.forename: David
authors.initials: D
authors.lastname: Adler
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yoshinaga
authors.forename: Maria
authors.initials: M
authors.lastname: Martinez
authors.forename: Michael
authors.initials: M
authors.lastname: Nefadov
authors.forename: Abul K
authors.initials: AK
authors.lastname: Abbas
authors.forename: Art
authors.initials: A
authors.lastname: Weiss
authors.forename: Lewis L
authors.initials: LL
authors.lastname: Lanier
authors.forename: Pieter J
authors.initials: PJ
authors.lastname: de Jong
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Bluestone
authors.forename: Deepak
authors.initials: D
authors.lastname: Srivastava
authors.forename: Michael T
authors.initials: MT
authors.lastname: McManus
mesh_terms: D000818:Animals; D053595:Embryonic Stem Cells; D020869:Gene Expression Profiling; D017930:Genes, Reporter; D005838:Genotype; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D023421:Models, Animal; D008957:Models, Genetic
chemical_list: D035683:MicroRNAs