title: Altered patterns of filopodia production in CHO cells heterologously expressing zebra finch CB(1) cannabinoid receptors.
abstract: Recent findings indicate that cannabinoid-altered vocal development involves elevated densities of dendritic spines in a subset of brain regions involved in zebra finch song learning and production suggesting that cannabinoid receptor activation may regulate cell structure. Here we report that activation of zebra finch CB 1 receptors (zfCB 1, delivered by a lentivector to CHO cells) produces dose-dependent biphasic effects on the mean length of filopodia expressed: Low agonist concentrations (3 nM WIN55212-2) increase lengths while higher concentrations reduce them. In contrast, treatment of zfCB 1-expressing cells with the antagonist/inverse agonist SR141716A causes increases in both mean filopodia length and number at 30 and 100 nM. These results demonstrate that CB 1 receptor activation can differentially influence filiopodia elongation depending on dose, and demonstrate that manipulation of cannabinoid receptor activity is capable of modulating cell morphology.
authors.affiliation: Department of Pharmacology and Toxicology, Brody School of Medicine, East Carolina University, Greenville, NC, USA. soderstromk@ecu.edu
authors.forename: Ken
authors.initials: K
authors.lastname: Soderstrom
authors.forename: Yuguo
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ashley R
authors.initials: AR
authors.lastname: Wilson
mesh_terms: D000818:Animals; D048588:Benzoxazines / Q000494:pharmacology; D016466:CHO Cells; D006224:Cricetinae; D046369:Finches / Q000254:growth & development* / Q000378:metabolism*; D018613:Microscopy, Confocal; D009025:Morpholines / Q000494:pharmacology; D009281:Naphthalenes / Q000494:pharmacology; D011554:Pseudopodia / Q000187:drug effects / Q000378:metabolism*; D043884:Receptor, Cannabinoid, CB1 / Q000819:agonists / Q000378:metabolism*
chemical_list: D048588:Benzoxazines; D009025:Morpholines; D009281:Naphthalenes; D043884:Receptor, Cannabinoid, CB1; C070417:(3R)-((2,3-dihydro-5-methyl-3-((4-morpholinyl)methyl)pyrrolo-(1,2,3-de)-1,4-benzoxazin-6-yl)(1-naphthalenyl))methanone