title: Comprehensive proteome analysis in Cenococcum geophilum Fr. as a tool to discover drought-related proteins.
abstract: Cenococcum geophilum is a widely distributed ectomycorrhizal fungus potentially playing a significant role in resistance and resilience mechanisms of its tree hosts exposed to drought stress. In this study, we performed a large scale protein analysis in pure cultures of C. geophilum in order to gain first global insights into the proteome assembly of this fungus. Using 1-D gel electrophoresis coupled with ESI-MS/MS, we indentified 638 unique proteins. Most of these proteins were related to the metabolic and cellular processes, and the transport machinery of cells. In a second step, we examined the influence of water deprivation on the proteome of C. geophilum pure cultures at three time points of gradually imposed drought. The results indicated that 12 proteins were differentially abundant in mycelia subjected to drought compared to controls. The induced responses in C. geophilum point towards regulation of osmotic stress, maintainance of cell integrity, and counteracting increased levels of reactive oxygen species formed during water deprivation.
authors.affiliation: Section of Forest Genetics, Technische Universität München, Hans-Carl-von-Carlowitz-Platz 2, D-85354 Freising, Germany. renekerner@hotmail.com
authors.forename: René
authors.initials: R
authors.lastname: Kerner
authors.forename: Edgar
authors.initials: E
authors.lastname: Delgado-Eckert
authors.forename: Estela
authors.initials: E
authors.lastname: Del Castillo
authors.forename: Gerhard
authors.initials: G
authors.lastname: Müller-Starck
authors.forename: Martina
authors.initials: M
authors.lastname: Peter
authors.forename: Bernhard
authors.initials: B
authors.lastname: Kuster
authors.forename: Emilie
authors.initials: E
authors.lastname: Tisserant
authors.forename: Karin
authors.initials: K
authors.lastname: Pritsch
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D055864:Droughts; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D020543:Proteome / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D020543:Proteome