title: Differentiation of polycystic ovary syndrome-derived human embryonic stem cells into adipocytes and their glucose consumption.
abstract: BACKGROUND
  Polycystic ovary syndrome (PCOS) is related to genetic factors. Adipose tissue and insulin resistance (IR) may play an important role in the pathogenesis and progression of PCOS. We investigate glucose consumption and insulin response abilities in the adipocytes differentiated from PCOS-derived human embryonic stem cells (hESCs) in vitro in order to provide a new idea for exploring PCOS pathogenesis.
  
  
  METHODS
  hESC lines were established with the discarded embryos of non-PCOS or PCOS-women, and then were differentiated into adipocytes. The glucose consumption ability and insulin response ability for these adipocytes were detected.
  
  
  RESULTS
  There was no significant difference in glucose consumption ability between non-PCOS and PCOS-derived adipocytes at the absence of insulin. Insulin could significantly increase glucose consumption abilities of both non-PCOS and PCOS-derived adipocytes, but there was no significant difference in the increased glucose consumption ability between the two types of adipocytes.
  
  
  CONCLUSIONS
  The glucose consumption ability and insulin response ability in the PCOS-derived adipocytes are similar to that in non-PCOS-derived adipocytes.
authors.affiliation: Reproductive Medical Centre, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Kong
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Liu
authors.forename: Ying-pu
authors.initials: YP
authors.lastname: Sun
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000502:physiology*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism; D051379:Mice; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D007328:Insulin; D005947:Glucose