title: Bioactivity-guided fractionation of the lipoxygenase and cyclooxygenase inhibiting constituents from Chisocheton polyandrus Merr.
abstract: Lipoxygenase (LOX)-inhibiting compounds from the leaves of Chisocheton polyandrus Merr. were isolated in this study using a bioactivity-guided fractionation technique. Two dammarane triterpenoids, dammara-20,24-dien-3-one (1) (IC(50)=0.69±0.07 μM) and 24-hydroxydammara-20,25-dien-3-one (2) (IC(50)=1.11±0.38 μM), were isolated and identified based on the soybean LOX assay. Dammara-20,24-dien-3-one (1) exhibited dual inhibition of both human 5-LOX (IC(50)=24.27±2.92 μM) and cyclooxygenase-2 (COX-2) (IC(50)=3.17±0.90 μM), whereas 24-hydroxydammara-20,25-dien-3-one (2) did not exhibit any significant inhibitory effects. This report is the first to detail the inhibition of LOX and COX by both C. polyandrus and its isolated compounds.
authors.affiliation: Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Kit Yee
authors.initials: KY
authors.lastname: Chan
authors.forename: Khalit
authors.initials: K
authors.lastname: Mohamad
authors.forename: Audrey J A
authors.initials: AJ
authors.lastname: Ooi
authors.forename: Zamrie
authors.initials: Z
authors.lastname: Imiyabir
authors.forename: Lip Yong
authors.initials: LY
authors.lastname: Chung
mesh_terms: D005591:Chemical Fractionation / Q000379:methods; D051546:Cyclooxygenase 2 / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008084:Lipoxygenase / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D029582:Meliaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D014315:Triterpenes; C575154:dammara-20,24-dien-3-one; D008084:Lipoxygenase; D051546:Cyclooxygenase 2