title: [Study on andrographolide-induced apoptosis of Candida albicans biofilm dispersion cells].
abstract: OBJECTIVE
  To detect the effect of andrographolide on apoptosis of Candida albicans biofilm dispersion cells.
  
  
  METHODS
  The morphological changes of apoptotic C. albicans biofilm cells were observed by using Hoechst 33258 staining Fluorescence microscope; changes of mitochondrial membrane potential (MMP) of C. albicans biofilm cells were detected by rhodamine 123 staining flow cytometry; and reactive oxygen species (ROS) was detected by DHR staining flow cytometry.
  
  
  RESULTS
  1 000, 100 micromol x L(-1) of andrographolide could cause pyknosis and dense staining of C. albicans biofilm cells, 1 000, 100, 10 micromol x L(-1) of andrographolide could decrease MMP and increase ROS of C. albicans biofilm cells.
  
  
  CONCLUSIONS
  Andrographolide of appropriate concentrations could induce apoptosis of dispersion cells of C. albicans biofilms.
authors.affiliation: School of Integrated Traditional and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China. ahwcz63@sina.com
authors.forename: Changzhong
authors.initials: C
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Han
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Xu
authors.forename: Huijuan
authors.initials: H
authors.lastname: Cheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Guan
authors.forename: Yun
authors.initials: Y
authors.lastname: Yun
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D018441:Biofilms; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology*; D004224:Diterpenes / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D004224:Diterpenes; D017382:Reactive Oxygen Species; C030419:andrographolide