title: Antioxidant supplementation in pregnant women with low antioxidant status.
abstract: OBJECTIVE
  The aim of this study was to investigate the benefit of antioxidant supplementation in a cohort of women with low antioxidant status and determine the changes in cell-free mRNA.
  
  
  METHODS
  This study was a randomized, placebo-controlled trial of 8-12 weeks' pregnant women who had low antioxidant status treated with either antioxidants or control diets daily until 2 weeks' postpartum. The primary end-point was the risk of pre-eclampsia and the secondary end-point was the changes of angiogenic and anti-oxidant mRNA markers related to the outcome (ClinicalTrial.gov, number NCT01232205).
  
  
  RESULTS
  There were 110 women enrolled in the study, randomly assigned to the supplementation (n = 52) and control group (n = 58). The overall rate of pre-eclampsia was 8.7% (nine subjects). There were significant differences (P = 0.034) between the supplementation and control group in the incidence of pre-eclampsia (2.0% [one case] and 14.5% [eight cases], respectively) and mRNA level of superoxide-dismutase, heme oxygenase-1, vascular endothelial growth factor receptor-1, endoglin and placental growth factor after supplementation.
  
  
  CONCLUSIONS
  Supplementation of women with low antioxidant status with micronutrients containing antioxidants during early gestation might reduce the risk of pre-eclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, University of Indonesia, Cipto Mangunkusumo National Hospital, Jakarta, Indonesia.
authors.forename: Noroyono
authors.initials: N
authors.lastname: Wibowo
authors.forename: Yuditiya
authors.initials: Y
authors.lastname: Purwosunu
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sekizawa
authors.forename: Antonio
authors.initials: A
authors.lastname: Farina
authors.forename: Liana
authors.initials: L
authors.lastname: Idriansyah
authors.forename: Ika
authors.initials: I
authors.lastname: Fitriana
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism / Q000627:therapeutic use*; D019587:Dietary Supplements / Q000706:statistics & numerical data*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D011225:Pre-Eclampsia / Q000097:blood / Q000517:prevention & control*; D011247:Pregnancy; D012333:RNA, Messenger / Q000097:blood; D055815:Young Adult
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger