title: Effects of recombinant gonadotropin hormones on the expression of vitellogenin, gonadotropin subunits and gonadotropin receptors in cinnamon clownfish, Amphiprion melanopus.
abstract: Gonadotropins (GTHs) are the key regulators of reproduction in vertebrates. The present study investigated autoregulatory effects of gonadotropins, using recombinant FSH (rFSH) and LH (rLH) in cinnamon clownfish (Amphiprion melanopus). Experiments were carried out to investigate the actions of cinnamon clownfish rFSH and rLH on expression of GTH subunits, GTH receptors, and vitellogenin (Vtg) mRNA in vivo and in vitro. Plasma estradiol-17β (E(2)) level was also measured in immature fish following treatments with rFSH and rLH. The results demonstrate increasing levels of GTH subunits, GTH-receptors, Vtg mRNA levels, as well as plasma E(2) levels following injection with rFSH and rLH. The findings support the hypothesis that LH and FSH stimulate reproduction, in part, by autoregulatory mechanisms leading to upregulation of GTH receptors and GTH hormone production in cinnamon clownfish. The results provide a framework for better understanding of the mechanisms of GTH-mediated control of reproduction in cinnamon clownfish and other vertebrates.
authors.affiliation: Division of Marine Environment & BioScience, Korea Maritime University, Busan 606-791, Republic of Korea.
authors.forename: Na Na
authors.initials: NN
authors.lastname: Kim
authors.forename: Hamid R
authors.initials: HR
authors.lastname: Habibi
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
authors.forename: Cheol Young
authors.initials: CY
authors.lastname: Choi
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000096:biosynthesis / Q000502:physiology; D037201:Follicle Stimulating Hormone, beta Subunit / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism; D007986:Luteinizing Hormone / Q000008:administration & dosage / Q000096:biosynthesis / Q000502:physiology; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics / Q000378:metabolism*; D008297:Male; D010473:Perciformes / Q000378:metabolism* / Q000502:physiology; D010902:Pituitary Gland / Q000378:metabolism; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism*; D011974:Receptors, LH / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000096:biosynthesis; D012098:Reproduction; D013737:Testis / Q000378:metabolism; D046509:Tissue Culture Techniques; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D037201:Follicle Stimulating Hormone, beta Subunit; D037101:Luteinizing Hormone, beta Subunit; D011962:Receptors, FSH; D011974:Receptors, LH; D011994:Recombinant Proteins; D014819:Vitellogenins; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone