title: Comparative proteomic analysis reveals differentially expressed proteins in Caenorhabditis elegans pgl-1 mutants grown at 20°C and 25°C.
abstract: PGL-1 is an RNA-binding protein component of germ granules and essential for fertility in Caenorhabditis elegans. To clarify the molecular function of PGL-1, we performed comparative proteomic analysis using 2-D DIGE and LC-MS/MS. Five groups of synchronized adult hermaphrodites were analyzed: (1) wild-type N2 grown at 20°C, (2) pgl-1(bn101) mutants grown at 20°C, (3) pgl-1(bn101) mutants grown at 20°C then upshifted to 25°C after the L1 stage, (4) pgl-1(ct131) mutants grown at 20°C, and (5) pgl-1(ct131) mutants grown at 20°C then upshifted to 25°C after the L1 stage. The five groups were divided into two experimental sets for 2-D DIGE: set A included N2 and pgl-1(bn101) mutants, and set B included N2 and pgl-1(ct131) mutants. Dunnett's test indicated 90 and 100 specific spots, respectively, with significantly different expression levels from the rest of the experimental set (q≤0.1). Among them, 69 and 58 spots, respectively, were analyzed by LC-MS/MS. Finally, we identified 19 proteins from 24 specific spots common to both the experimental sets. RNAi analysis indicated that decreased eef-1G expression is strongly associated with the temperature-sensitive sterile phenotype of pgl-1. Our results suggest that PGL-1 is closely involved in translational processes during C. elegans germline development.
authors.affiliation: Department of Bioinformatics, College of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japan.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Tohsato
authors.forename: Kanami
authors.initials: K
authors.lastname: Monobe
authors.forename: Kenji
authors.initials: K
authors.lastname: Suzuki
authors.forename: Toshiya
authors.initials: T
authors.lastname: Hayano
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kawasaki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ito
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins; D005786:Gene Expression Regulation; D009154:Mutation; D014176:Protein Biosynthesis; D020543:Proteome / Q000096:biosynthesis* / Q000235:genetics; D016601:RNA-Binding Proteins
chemical_list: D029742:Caenorhabditis elegans Proteins; C114543:PGL-1 protein, C elegans; D020543:Proteome; D016601:RNA-Binding Proteins