title: Construction of novel bifunctional chimeric proteins possessing antitumor and thrombolytic activities.
abstract: Based on their respective antitumor and thrombolytic activities, the superantigen staphylococcal enterotoxin C2 (SEC2) and staphylokinase (Sak) were chosen for the construction of the novel chimeric proteins Sak-linker- SEC2 and SEC2-linker-Sak using a linker composed of nine Ala residues. Both chimeric proteins possessed nearly the same PBMC proliferation stimulating activity and antitumor activity as SEC2 and thrombolytic activity as Sak. Neither the SEC2 or Sak component of each chimeric protein affected the activity of the other component. The results presented in this study provide a possible strategy to prevent and cure tumor thrombus.
authors.affiliation: Laboratory of Biomaterials and Biopharmaceuticals, School of Life Sciences, Liaoning University, Shenyang, 110036, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hui
authors.forename: Youjin
authors.initials: Y
authors.lastname: Dai
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Bian
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Cui
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Yu
authors.forename: Song
authors.initials: S
authors.lastname: You
authors.forename: Fengqing
authors.initials: F
authors.lastname: Hu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D002460:Cell Line; D004768:Enterotoxins / Q000235:genetics / Q000494:pharmacology*; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics / Q000494:pharmacology*; D009369:Neoplasms / Q000150:complications / Q000628:therapy; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000494:pharmacology; D013927:Thrombosis / Q000517:prevention & control
chemical_list: D000970:Antineoplastic Agents; D001688:Biological Products; D004768:Enterotoxins; D005343:Fibrinolytic Agents; D011993:Recombinant Fusion Proteins; C034959:enterotoxin C, staphylococcal; D008666:Metalloendopeptidases; C022653:auR protein, Staphylococcus aureus