title: N-3 PUFAs attenuate ischemia/reperfusion induced intestinal barrier injury by activating I-FABP-PPARγ pathway.
abstract: OBJECTIVE
  This study was designed to investigate whether n-3 PUFAs attenuate ischemia/reperfusion (I/R) induced intestinal barrier injury by activating I-FABP-PPARγ pathway.
  
  
  METHODS
  24 Male Sprague-Dawley rats were assigned to 4 groups: control group, I/R group, pretreated with n-3 PUFAs for 7 days before I/R (group 3), pretreated with peroxisome proliferator-activated receptor (PPARγ) agonist 30 min before I/R (group 4). The serum and intestinal mucosa samples were collected.
  
  
  RESULTS
  I/R disrupted the structure of intestinal tight junctions (TJs) and reduced occludin expression. The intestinal fatty acid binding protein (I-FABP) was elevated in plasma while decreased in cells. PPARγ expression in nucleus of intestinal mucosa was attenuated. N-3 PUFAs attenuated the damaged TJ structure and elevated occludin, intracellular I-FABP and PPARγ expression. A PPARγ agonist had the same effect as n-3 PUFAs.
  
  
  CONCLUSIONS
  The intestinal barrier is severely damaged after I/R, which is related to the redistribution of I-FABP. Our findings firstly indicate that n-3 PUFAs protect the intestinal barrier by modifying intracellular I-FABP, activating the PPARγ pathway, and then upregulating TJ protein expression.
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, 305 Zhongshan East Road, Nanjing 210002, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Wang
authors.forename: Liya
authors.initials: L
authors.lastname: Pan
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Jieshou
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology; D007422:Intestines / Q000187:drug effects* / Q000473:pathology*; D008297:Male; D046529:Microscopy, Electron, Transmission; D009698:Nucleoproteins / Q000302:isolation & purification / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C493407:Fabp2 protein, rat; D050556:Fatty Acid-Binding Proteins; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D009698:Nucleoproteins; D047495:PPAR gamma