title: Oleifolioside A mediates caspase-independent human cervical carcinoma HeLa cell apoptosis involving nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG.
abstract: Apoptosis, the main type of programmed cell death, plays an essential role in a variety of biological events. Whereas "classical" apoptosis is dependent on caspase activation, caspase-independent death is increasingly recognized as an alternative pathway. To develop new anticancer agents, oleifolioside A was isolated from Dendropanax morbifera Leveille and the biochemical mechanisms of oleifolioside A-induced apoptosis in HeLa cells were investigated. Exposure to oleifolioside A resulted in caspase activation and typical features of apoptosis, although cell death was not prevented by caspase inhibition. Oleifolioside A treatment induced up-regulation of Bad, loss of mitochondrial membrane potential, nuclear relocation of mitochondrial factors, apoptosis-inducing factor (AIF), endonuclease G (EndoG), and apoptosis induction. This is the first report of anticancer activity of oleifolioside A, and nuclear translocation of AIF and EndoG in oleifolioside A-treated HeLa cells might represent an alternative death signaling pathway in the absence of caspase activity.
authors.affiliation: College of Natural Resources and Life Science, BK21 Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: Hai Yang
authors.initials: HY
authors.lastname: Yu
authors.forename: Cheng-Yun
authors.initials: CY
authors.lastname: Jin
authors.forename: Kyoung-Sook
authors.initials: KS
authors.lastname: Kim
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
authors.forename: Shin-Hyung
authors.initials: SH
authors.lastname: Park
authors.forename: Gi-Young
authors.initials: GY
authors.lastname: Kim
authors.forename: Wun-Jae
authors.initials: WJ
authors.lastname: Kim
authors.forename: Hyung-In
authors.initials: HI
authors.lastname: Moon
authors.forename: Yung Hyun
authors.initials: YH
authors.lastname: Choi
authors.forename: Jai-Heon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D051033:Apoptosis Inducing Factor / Q000378:metabolism*; D027481:Araliaceae / Q000737:chemistry*; D020169:Caspases / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D021381:Protein Transport; D012503:Saponins / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D051033:Apoptosis Inducing Factor; D010936:Plant Extracts; D012503:Saponins; C546794:oleifolioside A; D004706:Endodeoxyribonucleases; C052391:endonuclease G; D020169:Caspases