title: Increased expression of regulatory T cells and down-regulatory molecules in lepromatous leprosy.
abstract: T regulatory cells (Tregs) play an important role in the mechanism of host's failure to control pathogen dissemination in severe forms of different chronic granulomatous diseases, but their role in leprosy has not yet been elucidated; 28 newly diagnosed patients (16 patients with lepromatous leprosy and 12 patients with tuberculoid leprosy) and 6 healthy Mycobacterium leprae-exposed individuals (contacts) were studied. Tregs were quantified by flow cytometry (CD4+ CD25+ Foxp3+) in peripheral blood mononuclear cells stimulated in vitro with a M. leprae antigenic preparation and phytohemagglutinin as well as in skin lesions by immunohistochemistry. The lymphoproliferative (LPR), interleukin-10 (IL-10), and interferon-γ (IFN-γ) responses of the in vitro-stimulated peripheral blood mononuclear cells and the in situ expression of IL-10, transforming growth factor-β (TGF-β), and cytotoxic T-lymphocyte antigen 4 (CTLA-4) were also determined. We show that M. leprae antigens induced significantly lower LPR but significantly higher Treg numbers in lepromatous than tuberculoid patients and contacts. Mitogen-induced LPR and Treg frequencies were not significantly different among the three groups. Tregs were also more frequent in situ in lepromatous patients, and this finding was paralleled by increased expression of the antiinflammatory molecules IL-10 and CTLA-4 but not TGF-β. In lepromatous patients, Tregs were intermingled with vacuolized hystiocyte infiltrates all over the lesion, whereas in tuberculoid patients, Tregs were rare. Our results suggest that Tregs are present in increased numbers, and they may have a pathogenic role in leprosy patients harboring uncontrolled bacillary multiplication but not in those individuals capable of limiting M. leprae growth.
authors.affiliation: Laboratory of Medical Investigation Unit 56, Division of Clinical Dermatology, Medical School, University of Sao Paulo, Sao Paulo, Brazil. luli_neves@yahoo.com.br
authors.forename: Maria L
authors.initials: ML
authors.lastname: Palermo
authors.forename: Carla
authors.initials: C
authors.lastname: Pagliari
authors.forename: Maria Angela B
authors.initials: MA
authors.lastname: Trindade
authors.forename: Tania M
authors.initials: TM
authors.lastname: Yamashitafuji
authors.forename: Alberto José S
authors.initials: AJ
authors.lastname: Duarte
authors.forename: Camila R
authors.initials: CR
authors.lastname: Cacere
authors.forename: Gil
authors.initials: G
authors.lastname: Benard
mesh_terms: D000328:Adult; D060908:CTLA-4 Antigen / Q000378:metabolism; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D054884:Host-Pathogen Interactions; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015440:Leprosy, Lepromatous / Q000276:immunology* / Q000503:physiopathology; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009166:Mycobacterium leprae / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D060908:CTLA-4 Antigen; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D007371:Interferon-gamma