title: Involvement of ganglioside GT1b in glutamate release from neuroblastoma cells.
abstract: Since gangliosides play many important roles in neural systems, we investigated whether gangliosides are involved in glutamate release from neural cells. Differentiated neruro2a cells were treated with gangliosides, including GM3, GM1, GD1a, GD3, GD1b, or GT1b, for 30 min, and glutamate concentration in the culture media was measured using o-phthalaldehyde derivatization. Among the tested gangliosides, GT1b significantly increased the glutamate concentration when compared with untreated cells. Moreover, GT1b increased the glutamate concentration in the culture media of neuroblastoma × dorsal root ganglion neuron hybrid F11 cells. These results suggested that gangliosides are important in regulating extracellular glutamate concentration in the nervous system.
authors.affiliation: Institute for Environmental and Gender-Specific Medicine, Juntendo University School of Medicine, 2-1-1 Tomioka, Urayasu-shi, 279-0021 Chiba, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Watanabe
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Higashi
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Takamori
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Iwabuchi
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D005732:Gangliosides / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors; D005971:Glutamates / Q000378:metabolism*; D006801:Humans; D006822:Hybrid Cells; D009025:Morpholines / Q000494:pharmacology; D009447:Neuroblastoma / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D004791:Enzyme Inhibitors; D005732:Gangliosides; D005971:Glutamates; D009025:Morpholines; C016481:trisialoganglioside GT1; C033110:RV 538; D005964:Glucosyltransferases; C023246:ceramide glucosyltransferase