title: [Establishment of a hydrogel chip for high-throughput detection of Y chromosome microdeletions].
abstract: OBJECTIVE
  To establish a high-sensitivity, high-specificity and low-cost hydrogel chip platform for the clinical screening of Y chromosome microdeletions.
  
  
  METHODS
  Site-specific extended primers with a common sequence at the 5' end were used for hybridizing with the target. The Cy5-dUTP was incorporated into the products by primer extension, and the products were labeled with fluorescence. Then the extended products were added to the chip for hybridizing with acrylamide-modified common probes immobilized on the chip. After removal of the free Cy5-dUTP by electrophoresis, the signals were obtained by fluorescence scanning. And the detecting conditions of this method were optimized.
  
  
  RESULTS
  SY254 of 9 samples was successfully detected with the hydrogel chip. The results showed that 3 were normal and the other 6 with microdeletions (1 female sample as a negative control), which coincided with the results of conventional multiplex PCR-electrophoresis.
  
  
  CONCLUSIONS
  The hydrogel chip platform we established has provided a new technique for the detection of Y chromosome microdeletions, and is beneficial to the diagnosis and treatment of male infertility.
authors.affiliation: College of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, China.
authors.forename: You-Zhi
authors.initials: YZ
authors.lastname: Li
authors.forename: Zhi-Yao
authors.initials: ZY
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Huan
authors.initials: H
authors.lastname: Huang
authors.forename: Qin-Xin
authors.initials: QX
authors.lastname: Song
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Zhou
mesh_terms: D002232:Carbocyanines; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics; D003856:Deoxyuracil Nucleotides; D006801:Humans; D020100:Hydrogels; D007248:Infertility, Male; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012729:Sex Chromosome Aberrations; D058533:Sex Chromosome Disorders of Sex Development / Q000175:diagnosis / Q000235:genetics*
chemical_list: C088942:3'-deoxy-5-(cyanine dye 5)uridine 5'-trisphosphate; D002232:Carbocyanines; D003856:Deoxyuracil Nucleotides; D020100:Hydrogels