title: Accumulation of trospectomycin by strains of Salmonella typhimurium, Escherichia coli and Haemophilus influenzae.
abstract: Trospectomycin, unlike aminoglycosidic aminocyclitols, is accumulated by a nonsaturable, energy-independent, diffusional process in Salmonella typhimurium, Escherichia coli and Haemophilus influenzae. A deep rough mutant of S typhimurium was more susceptible and accumulated the drug faster, and F porin deficient mutants of E coli were more resistant than parental strains. Trospectomycin likely uses both porin and nonporin pathways to cross the outer membrane. An E coli strain effectively accumulated the drug anaerobically, explaining its anaerobic activity. An H influenzae strain accumulated trospectomycin at concentrations below those for which detectable uptake could be observed with E coli or S typhimurium strains, consistent with greater activity in Haemophilus species.
authors.affiliation: Departments of Microbiology and Infectious Diseases, University of Calgary and Foothills Hospital, Calgary, Alberta.
authors.forename: S
authors.initials: S
authors.lastname: Wong
authors.forename: L
authors.initials: L
authors.lastname: Bryan