title: Statistical organelle dissection of Arabidopsis guard cells using image database LIPS.
abstract: To comprehensively grasp cell biological events in plant stomatal movement, we have captured microscopic images of guard cells with various organelles markers. The 28,530 serial optical sections of 930 pairs of Arabidopsis guard cells have been released as a new image database, named Live Images of Plant Stomata (LIPS). We visualized the average organellar distributions in guard cells using probabilistic mapping and image clustering techniques. The results indicated that actin microfilaments and endoplasmic reticulum (ER) are mainly localized to the dorsal side and connection regions of guard cells. Subtractive images of open and closed stomata showed distribution changes in intracellular structures, including the ER, during stomatal movement. Time-lapse imaging showed that similar ER distribution changes occurred during stomatal opening induced by light irradiation or femtosecond laser shots on neighboring epidermal cells, indicating that our image analysis approach has identified a novel ER relocation in stomatal opening.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha Kashiwa, Chiba 277-8562, Japan. takumi.higaki@gmail.com
authors.forename: Takumi
authors.initials: T
authors.lastname: Higaki
authors.forename: Natsumaro
authors.initials: N
authors.lastname: Kutsuna
authors.forename: Yoichiroh
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: Kae
authors.initials: K
authors.lastname: Akita
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ebine
authors.forename: Takashi
authors.initials: T
authors.lastname: Ueda
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kondo
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Hasezawa
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008027:Light; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D015388:Organelles / Q000378:metabolism*; D054046:Plant Stomata / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D030821:Plants, Genetically Modified; D025341:Principal Component Analysis; D021381:Protein Transport / Q000528:radiation effects; D059008:Time-Lapse Imaging
chemical_list: D029681:Arabidopsis Proteins; D008164:Luminescent Proteins