title: HLA-C polymorphisms in two cohorts of donors for bone marrow transplantation.
abstract: The typing for HLA-C in transplantation was rather neglected in the past. However, several recent studies have emphasized its role in transplantation and its association with the outcome. Serological typing of HLA-C could identify only a limited number of HLA-C antigens, resulting in a number of HLA-C blanks. This was mainly due to the low expression of surface HLA-C and the small number of available specific anti-sera. Performing molecular methods has identified new HLA-C alleles and filled the blank of most serological typed antigens. In this study, we compared serological and molecular typing of HLA-C in two cohorts of healthy Saudis. Our serological typing method identified HLA-C1-7 with different frequencies, 23.5% of the alleles were not identified and thus defined as blank. Using the SSP molecular method, all samples were typed and all alleles were defined. Both methods showed that C∗07 and C∗06 have the highest frequency in the Saudi population. Our study emphasizes the importance of molecular methods in identifying all possible HLA-C alleles.
authors.affiliation: King Abdullah International Medical Research Centre, Kingdom of Saudi Arabia.
authors.forename: Dunia
authors.initials: D
authors.lastname: Jawdat
authors.forename: Salwa
authors.initials: S
authors.lastname: Al Saleh
authors.forename: Paul
authors.initials: P
authors.lastname: Sutton
authors.forename: Hanan
authors.initials: H
authors.lastname: Al Anazi
authors.forename: Abdullah
authors.initials: A
authors.lastname: Shubaili
authors.forename: F Aytul
authors.initials: FA
authors.lastname: Uyar
authors.forename: Ali H
authors.initials: AH
authors.lastname: Hajeer
mesh_terms: D016026:Bone Marrow Transplantation; D015331:Cohort Studies; D005787:Gene Frequency; D015236:HLA-C Antigens / Q000235:genetics* / Q000276:immunology; D006579:Heterozygote; D006650:Histocompatibility Testing / Q000379:methods; D006720:Homozygote; D006801:Humans; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012189:Retrospective Studies; D012529:Saudi Arabia; D012698:Serologic Tests; D014019:Tissue Donors
chemical_list: D015236:HLA-C Antigens