title: Microsatellite marker-based identification and genetic  relationships of olive cultivars from the Extremadura region of Spain.
abstract: Seventy-seven olive accessions corresponding to 25  cultivars from the Extremadura region of Spain were studied using four  microsatellite or SSR markers in order to fingerprint them, and evaluate  genetic similarity and relationships between local and introduced olive  cultivars. The number of alleles per locus ranged from 4 to 8, with a mean of  6.25 alleles per primer pair (a total of 25 alleles). The observed  heterozygosity ranged from 0.58 to 0.95, while the expected heterozygosity  varied between 0.68 and 0.83. The polymorphism information content values  ranged from 0.63 to 0.79. The mean polymorphism information content value of  0.70 for the SSR loci provided sufficient discriminating ability to evaluate  the genetic diversity among the cultivars. The SSR data allowed unequivocal  identification of all the cultivars; a combination of three SSR markers was  sufficient to discriminate all 25 olive cultivars. A dendrogram was prepared,  using the unweighted pair-group method with arithmetic mean clustering  algorithm; it depicted the pattern of relationships between the cultivars. Most  of the local cultivars grouped according to their geographic origin. No clear  clustering trends were observed when the morphological traits of fruit  endocarps or fruit use of cultivars were employed as analysis criteria. We  conclude that there is a high level of variability among local olive cultivars  from the Extremadura region at both the morphological and molecular levels;  these data should be useful for identifying and distinguishing local germplasm.
authors.affiliation: Plant Physiology, Faculty of Science, University of Extremadura, Badajoz, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Delgado-Martinez
authors.forename: I
authors.initials: I
authors.lastname: Amaya
authors.forename: J F
authors.initials: JF
authors.lastname: Sánchez-Sevilla
authors.forename: M C
authors.initials: MC
authors.lastname: Gomez-Jimenez
mesh_terms: D000483:Alleles; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D031658:Olea / Q000235:genetics*; D011110:Polymorphism, Genetic; D013030:Spain
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers