title: Protein tyrosine phosphatase profiling analysis of HIB-1B cells during brown adipogenesis.
abstract: A number of evidence have been accumulated that the regulation of reversible tyrosine phosphorylation, which can be regulated by the combinatorial activity of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs), plays crucial roles in various biological processes including differentiation. There are a total of 107 PTP genes in the human genome, collectively referred to as the "PTPome." In this study, we performed PTP profiling analysis of the HIB-1B cell line, a brown preadipocyte cell line, during brown adipogenesis. Through RT-PCR and real-time PCR, several PTPs showing differential expression pattern during brown adipogenesis were identified. In the case of PTP-RE, it was shown to decrease significantly until 4 days after brown adipogenic differentiation, followed by a dramatic increase at 6 days. The overexpression of PTP-RE led to decreased brown adipogenic differentiation via reducing the tyrosine phosphorylation of the insulin receptor, indicating that PTP-RE functions as a negative regulator at the early stage of brown adipogenesis.
authors.affiliation: Medical Proteomics Research Center, KRIBB, Daejeon 305-806, Korea.
authors.forename: Hye-Ryung
authors.initials: HR
authors.lastname: Choi
authors.forename: Won Kon
authors.initials: WK
authors.lastname: Kim
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Hyeyun
authors.initials: H
authors.lastname: Jung
authors.forename: Jeong-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Baek-Soo
authors.initials: BS
authors.lastname: Han
authors.forename: Kwan-Hee
authors.initials: KH
authors.lastname: You
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Lee
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
mesh_terms: D050156:Adipogenesis; D002454:Cell Differentiation; D002460:Cell Line; D020869:Gene Expression Profiling; D006801:Humans; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017027:Protein Tyrosine Phosphatases