title: Comparative study of different alcohol sensors based on Screen-Printed Carbon Electrodes.
abstract: Different very simple single-use alcohol enzyme sensors were developed using alcohol oxidase (AOX) from three different yeast, Hansenula sp., Pichia pastoris and Candida boidinii, and employing three different commercial mediator-based Screen-Printed Carbon Electrodes as transducers. The mediators tested, Prussian Blue, Ferrocyanide and Co-phthalocyanine were included into the ink of the working electrode. The procedure to obtain these sensors consists of the immobilization of the enzyme on the electrode surface by adsorption. For the immobilization, an AOX solution is deposited on the working electrode and left until dried (1h) at room temperature. The best results were obtained with the biosensor using Screen-Printed Co-phthalocyanine/Carbon Electrode and AOX from Hansenula sp. The reduced cobalt-phthalocyanine form is amperometrically detected at +0.4V (vs. Ag pseudo reference electrode). This sensor shows good sensitivity (1211 nA mM(-1)), high precision (2.1% RSD value for the slope value of the calibration plot) and wide linear response (0.05-1.00 mM) for ethanol determination. The sensor provides also accurate results for ethanol quantification in alcoholic drinks.
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, 33006 Oviedo, Spain.
authors.forename: Estefanía
authors.initials: E
authors.lastname: Costa Rama
authors.forename: Julien
authors.initials: J
authors.lastname: Biscay
authors.forename: María Begoña
authors.initials: MB
authors.lastname: González García
authors.forename: A
authors.initials: A
authors.lastname: Julio Reviejo
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Pingarrón Carrazón
authors.forename: Agustín
authors.initials: A
authors.lastname: Costa García
mesh_terms: D000429:Alcohol Oxidoreductases; D000434:Alcoholic Beverages / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D002175:Candida / Q000201:enzymology*; D002244:Carbon / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D000431:Ethanol / Q000032:analysis* / Q000378:metabolism; D005295:Ferrocyanides / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D010843:Pichia / Q000201:enzymology*; D012680:Sensitivity and Specificity
chemical_list: D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D007211:Indoles; D009942:Organometallic Compounds; C063633:cobalt phthalocyanine; D000431:Ethanol; D002244:Carbon; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; C000170:ferric ferrocyanide