title: Integrated detection of intrinsic fluorophores in live microbial cells using an array of thin film amorphous silicon photodetectors.
abstract: Two-dimensional fluorescence spectroscopy (2D FS) provides a non-invasive means to assess cell condition without the introduction of changes to the cell environment. The method relies on the measurement of the excitation-emission fluorescence intensity matrix of key intrinsic fluorophores, like aromatic amino acids, enzyme cofactors, and vitamins. Commonly used detection systems are complex, with multiple bandpass filters, and are hard to miniaturize. Here, an amorphous silicon photodetector array system integrated with amorphous silicon-carbon alloy filters designed to detect three key fluorophores - tryptophan (Trp), reduced nicotine adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) - is demonstrated. These intrinsic fluorophores were detected in pure solutions and also in suspended yeast cells. The array system was used to monitor changes in intrinsic fluorophore concentration when a yeast cell solution was subject to a thermal shock stress.
authors.affiliation: INESC Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Lisbon, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Jóskowiak
authors.forename: N
authors.initials: N
authors.lastname: Stasio
authors.forename: V
authors.initials: V
authors.lastname: Chu
authors.forename: D M F
authors.initials: DM
authors.lastname: Prazeres
authors.forename: J P
authors.initials: JP
authors.lastname: Conde
mesh_terms: D005182:Flavin-Adenine Dinucleotide / Q000032:analysis*; D005453:Fluorescence; D009243:NAD / Q000032:analysis*; D012440:Saccharomyces / Q000737:chemistry / Q000166:cytology; D012825:Silicon / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D014364:Tryptophan / Q000032:analysis*
chemical_list: D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D014364:Tryptophan; D012825:Silicon