title: Honeycomb form β-tricalcium phosphate induces osteogenesis by geometrical property with BMSC.
abstract: To establish an effective method for bone augmentation, we introduced a new honeycomb-like β-tricalcium phosphate (H-β-TCP) with BMP-2 as a scaffold, whose unique geometrical properties induce osteoblastic differentiation of autologous bone marrow mesenchymal stem cells (BMSCs). A total of six beagle dogs from 6 to 7 years old were used for this study. BMSCs were cultured with autologous serum and BMP-2 on H-β-TCP. Differentiation to osteoblasts was demonstrated in vitro and exo vivo. Scanning electron microscopy revealed formation and calcification of a matrix-like structure within the H-β-TCP tunnels in BMSC culture. Moreover, treatment of BMP-2 promoted osteoblastic differentiation of BMSCs in H-β-TCP in a diffusion chamber. These results indicated that H-β-TCP may be a useful tool for construction of functional artificial bone.
authors.affiliation: Department of Social Dentistry, Matsumoto Dental University, Nagano, Japan. kyagami@po.mdu.ac.jp
authors.forename: Kimitoshi
authors.initials: K
authors.lastname: Yagami
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shirota
authors.forename: Satoru
authors.initials: S
authors.lastname: Shintani
authors.forename: Mitsuori
authors.initials: M
authors.lastname: Mayahara
authors.forename: Mikio
authors.initials: M
authors.lastname: Nishizawa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yanagisawa
authors.forename: Rachel
authors.initials: R
authors.lastname: Sammons
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kuboki
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry* / Q000494:pharmacology*; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008954:Models, Biological; D008968:Molecular Conformation; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D016062:Porosity; D013499:Surface Properties; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate