title: Flavone ingredients can synergistically inhibit NDV infecting cell and improve ND vaccine's protective rate.
abstract: In order to screen better flavone prescriptions of anti-Newcastle disease virus (NDV), four flavone ingredients of epimedium flavones (EF), baikal skullcap root flavones (BSRF), wild dendranthema flower flavones (WDFF), and sanchi flavones (SF) screened in previous experiments and their prescriptions were added into chicken embryo fibroblast monolayer with three drug-adding modes respectively. The cellular A(570) values, the highest virus inhibitory rates and the score based on virus inhibitory rate were calculated to compare their antiviral activity. In immune protective test, the effects of three preparations (EF-BSRF, EF-SF-WDFF-BSRF and EF-WDFF-BSRF screened by the results in vitro experiment) on NDV infection were compared in chickens vaccinated with ND vaccine then challenged with NDV. Blood was regularly sampled for serum antibody titer determination. The pathogenic and dead statuses of chickens were clinically examined. The results indicated that the A(570) values of the nine prescriptions, especially the foresaid three prescriptions at almost all concentrations in three drug-adding modes were significantly higher than that of the virus control group. The foresaid three prescriptions presented at the top five of the highest virus inhibitory rate, and located at the highest three of the score rank. The antibody titers and protective rates of the three prescriptions groups were higher than that of VC group, especially EF-SF-WDFF-BSRF group showed significant difference. These results indicated that flavone prescriptions composed with suitable compatibility could possess synergistical action of antiviral effect, ES-SF-WDFF-BSRF prescription could inhibit the cellular infectivity of NDV, improve the protective effect of ND vaccine and would be expected to exploit into a new-type antiviral drug.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. liujiaguo@njau.edu.cn
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yuqing
authors.forename: Sun
authors.initials: S
authors.lastname: Zhan'ao
authors.forename: Liu
authors.initials: L
authors.lastname: Jiaguo
authors.forename: Wang
authors.initials: W
authors.lastname: Deyun
authors.forename: Zhang
authors.initials: Z
authors.lastname: Baokang
authors.forename: Yi
authors.initials: Y
authors.lastname: Fang
authors.forename: Fan
authors.initials: F
authors.lastname: Yunpeng
authors.forename: Liu
authors.initials: L
authors.lastname: Dan
authors.forename: Liu
authors.initials: L
authors.lastname: Xu
authors.forename: Liu
authors.initials: L
authors.lastname: Cui
authors.forename: Hu
authors.initials: H
authors.lastname: Yuanliang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000998:Antiviral Agents / Q000494:pharmacology*; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology / Q000821:virology; D047309:Flavones / Q000494:pharmacology*; D008297:Male; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000187:drug effects* / Q000276:immunology*; D010936:Plant Extracts / Q000494:pharmacology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000998:Antiviral Agents; D047309:Flavones; D010936:Plant Extracts; D014765:Viral Vaccines; C043562:flavone