title: Cardiac repair achieved by bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid patches in a rat of myocardial infarction model.
abstract: Bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid (BMSC/SH) patches were implanted into myocardial infarction (MI) rat hearts to investigate the efficacies of them on enhancing left ventricular (LV) remodeling and cardiac repair. 45 rats were divided into four groups: Sham, MI (MI hearts, induced by a cryo-injury technique), SH and BMSC/SH (MI hearts with implantations of SH and BMSC/SH patches, respectively). After eight weeks of post-implantation, the patches for the SH and BMSC/SH groups were intact and well adhered on the MI zones with no and minor immunological responses, respectively, examined by a CD68 marker, while severe inflammation on the zones was observed for the MI group. The SH group showed the efficacy of cardiac repair on MI zones. Moreover, BMSC/SH group significantly improved the wall thickness of LV, assessed by echocardiography, and had high viability of delivery BMSC, largely reduced apoptosis, significantly promoted neo-vascularization and stimulated the secretions of various paracrine factors such as VEGF, examined by real-time PCR, in MI zones compared with those of the SH and MI groups. In conclusion, the therapeutic efficacies of using BMSC/SH patches for repairing MI hearts were demonstrated by showing the advantages of both bioactive SH patches and BMSC-based therapy.
authors.affiliation: Department of Surgery, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei 100, Taiwan, ROC.
authors.forename: Nai-Hsin
authors.initials: NH
authors.lastname: Chi
authors.forename: Ming-Chia
authors.initials: MC
authors.lastname: Yang
authors.forename: Tze-Wen
authors.initials: TW
authors.lastname: Chung
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Chen
authors.forename: Nai-Kuan
authors.initials: NK
authors.lastname: Chou
authors.forename: Shoei-Shen
authors.initials: SS
authors.lastname: Wang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use; D016026:Bone Marrow Transplantation / Q000379:methods*; D002478:Cells, Cultured; D005349:Fibroins / Q000737:chemistry / Q000627:therapeutic use*; D048091:Guided Tissue Regeneration / Q000379:methods*; D006820:Hyaluronic Acid / Q000737:chemistry / Q000627:therapeutic use*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D001672:Biocompatible Materials; C482288:fibroin, silkworm; D006820:Hyaluronic Acid; D005349:Fibroins