title: Dynamic sieving capillary electrophoresis analysis of xylitol selenite-induced apoptosis in SMMC-7221 cells.
abstract: DNA ladder fragments, regarded as a biochemical hallmark of apoptosis, have been separated quickly and successfully by capillary electrophoresis. Inter-nucleosomal DNA fragmentations induced by xylitol selenite were determined for the first time, while hydroxypropylmethylcellulose (HPMC) was served as the sieving matrix in dynamic sieving capillary electrophoresis. The calibration curve (r(2) = 0.991) was established and multiples of two different nucleosomes (140 and 180 bp) were formed in the presence of xylitol selenite. Selenium compounds inhibited carcinogenesis in animal models, SMMC-7221 cells and several other cells by increasing apoptosis. The described method was useful in elucidating the anticancer activities of xylitol selenite and other selenium compounds, which was more effective to detect small fragments than slab gel electrophoresis.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation & Integrative Biology, College of Life Science, Central China Normal University, No. 152, Luoyu Road of Wuhan, Wuhan, Hubei Province, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.forename: Didi
authors.initials: D
authors.lastname: Chen
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Deng
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Layue
authors.initials: L
authors.lastname: Chen
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Hancao
authors.initials: H
authors.lastname: Yao
authors.forename: Guomei
authors.initials: G
authors.lastname: Xiong
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.forename: Jihong
authors.initials: J
authors.lastname: Yang
authors.forename: Chao
authors.initials: C
authors.lastname: Qi
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D053938:DNA Fragmentation; D019075:Electrophoresis, Capillary / Q000379:methods*; D005374:Filtration / Q000379:methods; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D065347:Hypromellose Derivatives; D008747:Methylcellulose / Q000031:analogs & derivatives / Q000737:chemistry; D018038:Sodium Selenite / Q000633:toxicity*; D014993:Xylitol / Q000633:toxicity*
chemical_list: D065347:Hypromellose Derivatives; D008747:Methylcellulose; D018038:Sodium Selenite; D014993:Xylitol