title: Development of screening assays for nanoparticle toxicity assessment in human blood: preliminary studies with charged Au nanoparticles.
abstract: As nanoparticles have found increased use in both consumer and medical applications, corresponding increases in possible exposure to humans necessitate studies examining the impacts of these nanomaterials in biological systems. This article examines the effects of approximately 30-nm-diameter gold nanoparticles, with positively and negatively charged surface coatings in human blood. Here, we study the exposure effects, with up to 72 h of exposure to 5, 15, 25 and 50 µg/ml nanoparticles on hemolysis, reactive oxygen species (ROS) generation and platelet aggregation in subsets of cells from human blood. Assessing viability with hemolysis, results show significant changes in a concentration-dependent fashion. Rates of ROS generation were investigated using the dichlorofluorscein diacetate-based assay as ROS generation is a commonly suspected mechanism of nanoparticle toxicity; herein, ROS was not a significant factor. Optical monitoring of platelet aggregation revealed that none of the examined nanoparticles induced aggregation upon short-term exposure.
authors.affiliation: Department of Chemistry, University of Minnesota, Kolthoff & Smith Halls, 207 Pleasant Street SE, Minneapolis, MN 55455, USA.
authors.forename: Sara A
authors.initials: SA
authors.lastname: Love
authors.forename: John W
authors.initials: JW
authors.lastname: Thompson
authors.forename: Christy L
authors.initials: CL
authors.lastname: Haynes
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D006046:Gold / Q000737:chemistry / Q000633:toxicity*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D010316:Particle Size; D010974:Platelet Aggregation / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000097:blood / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D006046:Gold