title: Identification and characterization of polymeric and metallic wear debris from periprosthetic tissues after total hip revision surgery.
abstract: In spite of the growing interest in the field of orthopedic wear debris, there is no standardized technique to simultaneously isolate and analyze both ultra-high-molecular-weight polyethylene (UHMWPE) and metallic debris from periprosthetic tissues. Using a modification of the previously employed base-digestion protocol involving solvent and mechanical treatment, we were able to separate the wear particles from the tissue. Subsequently, using environmental scanning electron microscopy (ESEM) and energy-dispersive spectrometry (EDS), we characterized individual particulate species. Metallic debris, particularly Co, Cr, and Mo, appeared as irregular and amorphous-like structures, whereas UHMWPE and Ti appeared as crystalline-like structures, some as small as 15 nm. The investigation revealed that UHMWPE forms the bulk (~82%), followed by Ti (~8%-9%), Co (~5%-6%), and Cr (~3%-4%), along with many other trace elements, such as Mo, Pb, Fe, Pb, Fe, Si (~1%-2%).
authors.affiliation: Department of Orthopaedic Surgery and Rehabilitation Medicine, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA.
authors.forename: Mrinal K
authors.initials: MK
authors.lastname: Musib
authors.forename: Vijay
authors.initials: V
authors.lastname: Rasquinha
authors.forename: Subrata
authors.initials: S
authors.lastname: Saha
mesh_terms: D019644:Arthroplasty, Replacement, Hip; D006622:Hip Prosthesis; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008670:Metals; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D010014:Osteolysis / Q000150:complications; D011095:Polyethylenes; D011475:Prosthesis Failure; D013057:Spectrum Analysis
chemical_list: D008670:Metals; D011095:Polyethylenes; C111601:ultra-high molecular weight polyethylene