title: Comparison of three immunosensor methods (surface plasmon resonance, screen-printed and classical amperometric immunosensors) for immunoglobulin G determination in human serum and animal or powdered milks.
abstract: Within the framework of research carried out by our team aimed at developing new immunological methods to determine proteins such as immunoglobulins G in different biological matrixes, for instance, serum and milk, tests were performed on several immunosensors based on different transducer types, i.e. amperometric (classical or screen-printed) electrodes for hydrogen peroxide. Lastly the feasibility of constructing immunosensors based on surface plasmon resonance (SPR) was investigated. "Competitive" immunological procedures were used in the first two cases. Conversely, the surface plasmon resonance transduction technique allowed a "direct" measurement. Applications were performed on human serum, powdered milks for babies and particularly on several animal milks, in the case of buffalo milk seeking a routine control method to identify possible inflammatory affections in the animals.
authors.affiliation: Dipartimento di Chimica, Università di Roma "La Sapienza", P.le Aldo Moro 5, 00185 Rome, Italy. mauro.tomassetti@uniroma1.it
authors.forename: Mauro
authors.initials: M
authors.lastname: Tomassetti
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Martini
authors.forename: Luigi
authors.initials: L
authors.lastname: Campanella
authors.forename: Gabriele
authors.initials: G
authors.lastname: Favero
authors.forename: Luciano
authors.initials: L
authors.lastname: Carlucci
authors.forename: Franco
authors.initials: F
authors.lastname: Mazzei
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019921:Biotinylation; D002020:Buffaloes; D004867:Equipment Design; D006041:Goats; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood*; D041943:Infant Formula / Q000737:chemistry; D008892:Milk / Q000737:chemistry* / Q000276:immunology; D011208:Powders; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D014159:Transducers
chemical_list: D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; D011208:Powders