title: [Role of galectin-1 on epithelial-to-mesenchymal transition induced by high glucose peritoneal dialysate in human peritoneal mesothelial cells].
abstract: OBJECTIVE
  To investigate the expression of galectin-1 with the stimulation of peritoneal dialysis solution (PDS) and its role in the epithelial-to-mesenchymal transition (EMT) in human peritoneal mesothelial cells (HPMCs).
  
  
  METHODS
  HPMCs were stimulated with PDS containing different concentrations of high glucose (1.5%, 2.5%, and 4.25%). After 24 h, mRNA and protein expressions of galectin-1,vimentin, and zo-1 were analyzed with real-time PCR and Western blot, respectively. Liposome transfected siRNA technique was used to knock down the expression of galectin-1 and the effect of galectin-1 siRNA on the EMT of HPMCs was also observed under 4.25% PDS condition.
  
  
  RESULTS
  mRNA expression of galectin-1 in HPMCs increased in PDS groups, especially in group with 4.25% PDS (P<0.05). Protein expression of galectin-1 in HPMCs significantly increased in PDS groups with a dose dependent manner (P<0.05).Expression of vimentin in HPMCs significantly increased in PDS groups, especially in groups of 2.5% PDS and 4.25% PDS (P<0.05), but zo-1 expression markedly decreased (P<0.05). The expression of galectin-1 correlated positively with vimentin (P<0.05) but negatively with zo-1 (P<0.05). Expression of vimentin in groups of 4.25% PDS was markedly inhibited (P<0.05) by galectin-1 siRNA, whereas zo-1 expression was significantly increased (P<0.05).
  
  
  CONCLUSIONS
  Galectin-1 can mediate high glucose PDS-induced EMT in HPMCs and may be a new target for the prevention and treatment of peritoneal fibrosis.
authors.affiliation: Department of Nephropathy, Central South University, Changsha, China. liuyingh2002@yahoo.com.cn
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Liu
authors.forename: Hongqin
authors.initials: H
authors.lastname: Dai
authors.forename: Fuyou
authors.initials: F
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D002478:Cells, Cultured; D015314:Dialysis Solutions / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D037483:Galectin 1 / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D010530:Peritoneal Dialysis / Q000009:adverse effects; D056627:Peritoneal Fibrosis / Q000209:etiology; D010537:Peritoneum / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D015314:Dialysis Solutions; D037483:Galectin 1; C445499:LGALS1 protein, human; D012333:RNA, Messenger; D005947:Glucose