title: Assessing iPSC reprogramming methods for their suitability in translational medicine.
abstract: The discovery of the ability to induce somatic cells to a pluripotent state through the overexpression of specific transcription factors has the potential to transform the ways in which pharmaceutical agents and cellular transplantation therapies are developed. Proper utilization of the technology to generate induced pluripotent stem cells (iPSCs) requires that researchers select the appropriate reprogramming method for generating iPSCs so that the resulting iPSCs can be transitioned towards clinical applications effectively. This article reviews all of the currently available reprogramming techniques with a focus on critiquing them on the basis of their utility in translational medicine.
authors.affiliation: National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Laboratory of Stem Cell Biology, Bethesda, MD, USA. raomah@mail.nih.gov
authors.forename: Mahendra S
authors.initials: MS
authors.lastname: Rao
authors.forename: Nasir
authors.initials: N
authors.lastname: Malik
mesh_terms: D002454:Cell Differentiation; D060846:Cell Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044968:Regenerative Medicine; D015203:Reproducibility of Results; D014162:Transfection; D019076:Transgenes; D057170:Translational Research, Biomedical / Q000379:methods*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger