title: Neuroprotective effect of fucoidin on lipopolysaccharide accelerated cerebral ischemic injury through inhibition of cytokine expression and neutrophil infiltration.
abstract: In our previous study, we reported that lipopolysaccharide (LPS) activated microglia and accelerated cerebral ischemic injury in the rat brain through the overexpression of cytokines in microglia. In the present study, we investigated the effect of the intraperitoneal administration of fucoidin, a potent inhibitor of leukocyte rolling and anti-inflammatory agent, against accelerated cerebral ischemic injury by LPS pretreatment using rats. We found that fucoidin treatment inhibited the expressions of some brain cytokine or chemokine mRNA such as IL-8, TNF-α and iNOS in the brain of the rats treated only with LPS. We also observed that fucoidin treatment dramatically decreased the infarct size in accelerated cerebral ischemic injury induced by LPS treatment at an early time after ischemic injury. In addition, the immunoreactivity of myleoperoxidase (MPO), a marker for quantifying neutrophil accumulation, was distinctively decreased in the ischemic brain of the fucoidin-treated rat. In brief, our results indicate that fucoidin showed a neuroprotective effect on LPS accelerated cerebral ischemic injury through inhibiting the expression of some cytokine/chemokine and neutrophil recruitments.
authors.affiliation: Department of Emergency Medicine, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, 150-950, Republic of Korea.
authors.forename: Gu Hyun
authors.initials: GH
authors.lastname: Kang
authors.forename: Bing Chun
authors.initials: BC
authors.lastname: Yan
authors.forename: Geum-Sil
authors.initials: GS
authors.lastname: Cho
authors.forename: Won-Ki
authors.initials: WK
authors.lastname: Kim
authors.forename: Choong Hyun
authors.initials: CH
authors.lastname: Lee
authors.forename: Jun Hwi
authors.initials: JH
authors.lastname: Cho
authors.forename: Missok
authors.initials: M
authors.lastname: Kim
authors.forename: Il-Jun
authors.initials: IJ
authors.lastname: Kang
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Lee
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000473:pathology* / Q000503:physiopathology*; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000235:genetics; D004195:Disease Models, Animal; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D020556:Neutrophil Infiltration / Q000187:drug effects* / Q000502:physiology; D011134:Polysaccharides / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016207:Cytokines; D018696:Neuroprotective Agents; D011134:Polysaccharides; C007789:fucoidan