title: The treatment of osteoporosis through transplantation of bone marrow stem cells in the experiments performed on rats.
abstract: The aim of our research was to study pathogenetic features of the development of experimental osteoporosis in rats and possibility of correction through stem cells of bone marrow. For modeling of osteoporosis was tested glucocorticoid model.  Experiments were conducted on 30 white male laboratory rats of Lewis line weighing 250-300 g. In the first group the experiment of reproduction of glucocorticoid model were performed (15 animals) by daily intraperitoneal injection of hydrocortisone at a dose of 40 mg/kg per day for 3 weeks. In the second group (15 animals) at 90 days after the creation of model of osteoporosis in the tail vain of the rat were injected 50 million cultured bone marrow stem cells. The control group of animals received intraperitoenal saline at the same volume. The maximum period of observation of the animals in both groups was 2 week, 1, 2, 3, 4, 6, months.  Pilot studies carried out at this stage have revealed that glucocorticoid model of osteoporosis is an adequate model for studying the mechanism of early development of osteoporosis and assessment of therapeutic effects. Cell therapy using bone marrow stem cells reduces the severity of osteoporosis, increases bone mineral density and increases strength of the bone by reducing the amount of the markers of resorption.
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology; D006854:Hydrocortisone / Q000008:administration & dosage*; D008297:Male; D010024:Osteoporosis / Q000139:chemically induced / Q000628:therapy*; D051381:Rats; D011917:Rats, Inbred Lew; D033581:Stem Cell Transplantation / Q000379:methods
chemical_list: D006854:Hydrocortisone