title: Preparation of icariside II-phospholipid complex and its absorption across Caco-2 cell monolayers.
abstract: In the present study, an icariside II-phospholipid complex was prepared, and its physicochemical properties including UV spectrum, IR spectrum, differential scanning calorimetry (DSC) were tested. Furthermore, the absorption of icariside II and icariside II-phospholipid complex was compared in a Caco-2 cell culture model. The results show that icariside II-phospholipid complex could significantly increase the A-B transport of icariside II (P < 0.01), with A-B Papp of (3.92 +/- 0.50) x 10(-6) cm/s compared to control (2.05 +/- 0.18) x 10(-6) cm/s (increased by 91%). Likewise, the B-A transport of icariside II was significantly enhanced (P < 0.01), with Papp of (15.3 +/- 0.72) x 10(-6) cm/s (control) to (22.4 +/- 1.4) x 10(-6) cm/s (46% increase). Efflux ratio of icariside II decreased by 23.5% after forming icariside II-phospholipid complex. The therefore, it could be concluded that phospholipid complexation can increase the intestinal absorption of icariside II.
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Materia Medical, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Zhen-Hai
authors.initials: ZH
authors.lastname: Zhang
authors.forename: E
authors.initials: E
authors.lastname: Sun
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Tan
authors.forename: Fen-Xia
authors.initials: FX
authors.lastname: Zhu
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Li
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Jia
mesh_terms: D000042:Absorption; D001693:Biological Transport, Active; D018938:Caco-2 Cells; D002152:Calorimetry, Differential Scanning; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003627:Data Interpretation, Statistical; D017097:Electric Impedance; D005419:Flavonoids / Q000737:chemistry*; D006801:Humans; D010743:Phospholipids / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005419:Flavonoids; D010743:Phospholipids; C060988:baohuoside I