title: Absence of anionic phospholipids in  Kluyveromyces lactis  cells is fatal without F1-catalysed ATP hydrolysis.
abstract: We have shown in previous research that the loss of phosphatidylglycerol and cardiolipin caused by disruption of the PGS1 gene is lethal for the petite-negative yeast  Kluyveromyces lactis . This present study demonstrates the role and mechanism of atp2.1 in the suppression of pgs1 lethality in K. lactis cells. Phenotypic characterization has shown that a strain lacking the phosphatidylglycerolphosphate synthase (atp2.1pgs1Δ) possessed a markedly impaired respiratory chain, very low endogenous respiration, and uncoupled mitochondria. As a result the mutant strain was unable to generate a sufficient mitochondrial membrane potential via respiration. The atp2.1 suppressor mutation enabled an increase in the affinity of F(1)-ATPase for ATP in the hydrolytic reaction, resulting in the maintenance of sufficient membrane potential for the biogenesis of mitochondria and survival of cells lacking anionic phospholipid biosynthesis.
authors.affiliation: Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B-2, Bratislava 842 15, Slovak Republic.
authors.forename: Viktoria
authors.initials: V
authors.lastname: Palovicova
authors.forename: Annamaria
authors.initials: A
authors.lastname: Bardelcikova
authors.forename: Margita
authors.initials: M
authors.lastname: Obernauerova
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002384:Catalysis; D004579:Electron Transport / Q000235:genetics; D006868:Hydrolysis; D007716:Kluyveromyces / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D013489:Suppression, Genetic
chemical_list: D010743:Phospholipids; D000251:Adenosine Triphosphatases