title: Isolation and characterization of a muskmelon cDNA encoding Lycopene Beta-cyclase.
abstract: Lycopene Beta-cyclase (LCY-B) is thought to play a critical role in Beta-carotene synthesis in fruit. A full-length cDNA clone encoding Lycopene Beta-cyclase was isolated from muskmelon (Cucumis melo L.) by RT-PCR and RACE. The clone, designated CmLcyb1, contains 1871 nucleotides, with an open reading frame of 1512 nucleotides. The deduced 504-amino-acid sequence showed high identities with other plant Lycopene Beta-cyclases. Real time quantitative RT-PCR analysis indicated that CmLcyb1 was expressed in all tissues and organs of muskmelon inbred M01-3 with white mesocarp and, 'Homoka', an orange mesocarp cultivar. The expression levels of CmLcyb1 in roots, stems, leaves and flowers in the two genotypes differed little. The expression level was highest in mature fruit of 'Homoka' and was much higher than that in mature fruit of M01-3. Moreover, the mRNA level of CmLcyb1 was very low in fruits before fruit-size fixation and increased dramatically in the size-fixed fruits of these two genotypes. The mRNA levels of CmLcyb1 during fruit development of 'Homoka' were all higher than those of M01-3. Interestingly, Beta-carotene content showed almost the same change trend as mRNA levels during fruit development in these two genotypes, suggesting that Beta-carotene accumulation may be linked to the CmLcyb1 transcript level in muskmelon fruit.
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Hao
authors.forename: Leyuan
authors.initials: L
authors.lastname: Ma
authors.forename: Hongzi
authors.initials: H
authors.lastname: Cong
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Xiyan
authors.initials: X
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D028463:Cucumis melo / Q000201:enzymology / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D019753:Intramolecular Lyases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D019753:Intramolecular Lyases; C070925:lycopene cyclase-isomerase