title: Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdickás reaction and Quartz Crystal Microbalance.
abstract: Treatment strategies for tumour diseases are progressively focusing on personalization of medicine. However, this focus requires methods revealing the early general biological mechanisms, including the formation anti-cancer drugs' resistance. The low molecular mass protein metallothionein is thought to be the crucial for the formation of resistance in tumour treatment based on the platinum-cytostatics. The interactions between metallothionein (MT) and cisplatin were determined by the adsorptive transfer stripping technique coupled with the differential pulse votlammetry Brdickás reaction. The signals related to the MT-cisplatin complex appeared at -0.9 V. The formation of this complex depended on the time of interaction between cisplatin and MT. The complex formation was consequently confirmed by quartz crystal microbalance analyses. The formation of this complex was detectable even after a 20 s long interaction. Moreover, we detected presence of MT-cisplatin complex in the blood of male rats treated with this drug.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
authors.forename: Dalibor
authors.initials: D
authors.lastname: Huska
authors.forename: Ivo
authors.initials: I
authors.lastname: Fabrik
authors.forename: Jiri
authors.initials: J
authors.lastname: Baloun
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.forename: Michal
authors.initials: M
authors.lastname: Masarik
authors.forename: Jaromir
authors.initials: J
authors.lastname: Hubalek
authors.forename: Anna
authors.initials: A
authors.lastname: Vasku
authors.forename: Libuse
authors.initials: L
authors.lastname: Trnkova
authors.forename: Ales
authors.initials: A
authors.lastname: Horna
authors.forename: Ladislav
authors.initials: L
authors.lastname: Zeman
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek