title: Crystal structure of periplasmic catecholate-siderophore binding protein VctP from Vibrio cholerae at 1.7 Å resolution.
abstract: VctP, one of the two essential siderophore-binding PBPs from the pathogen Vibrio cholerae, plays an important role in the transport of enterobactin and vibriobactin, which have quite different configurations of iron coordination, from the periplasm to the inner membrane. The current study reports the crystal structure of VctP from V. cholerae N16961 at 1.7Å resolution. A structural comparison of VctP with its homologues and the results of molecular docking indicate that enterobactin and vibriobactin share the same binding pocket. Significantly, a basic triad consisting of Arg137, Arg226 and Arg270 is used to balance the three negative charges of ferric-enterobactin, while a basic dyad consisting of Arg137 and Arg270 is used to balance the two negative charges of ferric-vibriobactin.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Du
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.forename: Shiheng
authors.initials: S
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Chunyuan
authors.initials: C
authors.lastname: Zhu
authors.forename: Deyu
authors.initials: D
authors.lastname: Zhu
authors.forename: Tiandi
authors.initials: T
authors.lastname: Wei
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Sujuan
authors.initials: S
authors.lastname: Xu
authors.forename: Lichuan
authors.initials: L
authors.lastname: Gu
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D002396:Catechols / Q000378:metabolism; D018360:Crystallography, X-Ray; D004758:Enterobactin / Q000378:metabolism; D008969:Molecular Sequence Data; D010080:Oxazoles / Q000378:metabolism; D033902:Periplasmic Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D017262:Siderophores / Q000378:metabolism*; D014734:Vibrio cholerae / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002396:Catechols; D010080:Oxazoles; D033902:Periplasmic Binding Proteins; D017262:Siderophores; D004758:Enterobactin; C040227:vibriobactin