title: Downregulation of DLEC1 in sinonasal inverted papilloma and squamous cell carcinoma.
abstract: OBJECTIVE
  Inverted papilloma is associated with sinonasal squamous cell carcinoma. This study aimed to investigate the epigenetic regulation of the tumour suppressor gene DLEC1 in inverted papilloma and sinonasal squamous cell carcinoma.
  
  
  METHODS
  DLEC1 gene expression was investigated by quantitative real-time polymerase chain reaction (QRT-PCR) in normal mucosa, inverted papilloma, and squamous cell carcinoma tissues. Methylation-specific PCR and subsequent autosequencing were also used to examine the methylation status of DLEC1 promoter and the involved mechanism. DLEC1 expression in a large validation set was evaluated by immunohistochemistry (n  =  25 in each group).
  
  
  RESULTS
  DLEC1 was downregulated in inverted papilloma and squamous cell carcinoma tissues compared to normal mucosa (p < .01 in QRT-PCR and immunohistochemistry), with squamous cell carcinoma more repressed (p < .05 in QRT-PCR and immunohistochemistry) than inverted papilloma tissues. DLEC1 promoter hypermethylation was found in squamous cell carcinoma tissues.
  
  
  CONCLUSIONS
  Repression of DLEC1 in squamous cell carcinoma tissues is associated with promoter hypermethylation. DLEC1 is downregulated in sinonasal squamous cell carcinoma and inverted papilloma and has a distinct mechanism.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Chang Gung Memorial Hospital, Chang Gung University, Taoyuan, 333, Taiwan.
authors.forename: Po-Hung
authors.initials: PH
authors.lastname: Chang
authors.forename: Chi-Che
authors.initials: CC
authors.lastname: Huang
authors.forename: Ta-Jen
authors.initials: TJ
authors.lastname: Lee
authors.forename: Yun-Shien
authors.initials: YS
authors.lastname: Lee
authors.forename: Chi-Neu
authors.initials: CN
authors.lastname: Tsai
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism / Q000473:pathology; D009669:Nose Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018308:Papilloma, Inverted / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010255:Paranasal Sinus Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D055815:Young Adult
chemical_list: C119064:DLEC1 protein, human; D012334:RNA, Neoplasm; D025521:Tumor Suppressor Proteins