title: Screening for a single-chain variable-fragment antibody that can effectively neutralize the cytotoxicity of the Vibrio parahaemolyticus thermolabile hemolysin.
abstract: Vibrio parahaemolyticus is  a halophilic bacterium that is widely distributed in water resources. The bacterium causes lethal food-borne diseases and poses a serious threat to human and animal health all over the world. The major pathogenic factor of V. parahaemolyticus is thermolabile hemolysin (TLH), encoded by the tlh gene, but its toxicity mechanisms are unknown. A high-affinity antibody that can neutralize TLH activity effectively is not available. In this study, we successfully expressed and purified the TLH antigen and discovered a high-affinity antibody to TLH, named scFv-LA3, by phage display screening. Cytotoxicity analysis showed that scFv-LA3 has strong neutralization effects on TLH-induced cell toxicity.
authors.affiliation: The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology and the College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Rongzhi
authors.initials: R
authors.lastname: Wang
authors.forename: Sui
authors.initials: S
authors.lastname: Fang
authors.forename: Dinglong
authors.initials: D
authors.lastname: Wu
authors.forename: Junwei
authors.initials: J
authors.lastname: Lian
authors.forename: Jue
authors.initials: J
authors.lastname: Fan
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shihua
authors.initials: S
authors.lastname: Wang
authors.forename: Wenxiong
authors.initials: W
authors.lastname: Lin
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000915:Antibody Affinity; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D002460:Cell Line; D061505:Cell Surface Display Techniques; D006367:HeLa Cells; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D057127:Single-Chain Antibodies / Q000276:immunology*; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D057134:Antibodies, Neutralizing; D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D057127:Single-Chain Antibodies; C073845:hemolysin, Vibrio