title: Immunological role of thiol-dependent peroxiredoxin gene in Macrobrachium rosenbergii.
abstract: In this study, we have reported a full length of peroxiredoxin (designated MrPrdx) gene, identified from the transcriptome of freshwater prawn Macrobrachium rosenbergii. The complete gene sequence of the MrPrdx is 940 base pairs in length, and encodes 186 amino acids. MrPrdx contains a long thioredoxin domain in the amino acid sequence between 34 and 186. The gene expressions of MrPrdx in healthy and the infectious hypodermal and hematopoietic necrosis virus (IHHNV) challenged M. rosenbergii were examined using quantitative real time polymerase chain reaction. MrPrdx is highly expressed in all the other tissues of M. rosenbergii considered for analysis and the highest in gills. The expression is strongly up-regulated in gills after IHHNV infection. To understand MrPrdx functional properties, the recombinant MrPrdx protein was expressed in Escherichia coli BL21 (DE3) and purified. A peroxidise activity assay was conducted using recombinant MrPrdx protein at different concentrations. This peroxidises activity showed that the recombinant MrPrdx is a thiol-dependant protein. Additionally, this result showed that recombinant MrPrdx protein, as a secretory protein can remove H₂O₂ and protect DNA damage. This finding leads a possible way to propose the recombinant MrPrdx protein as an effective medicine for reactive oxygen species (ROS) related diseases.
authors.affiliation: Centre for Biotechnology in Agriculture Research, Division of Genetics & Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Jesu
authors.initials: J
authors.lastname: Arockiaraj
authors.forename: Sarasvathi
authors.initials: S
authors.lastname: Easwvaran
authors.forename: Puganeshwaran
authors.initials: P
authors.lastname: Vanaraja
authors.forename: Arun
authors.initials: A
authors.lastname: Singh
authors.forename: Rofina Yasmin
authors.initials: RY
authors.lastname: Othman
authors.forename: Subha
authors.initials: S
authors.lastname: Bhassu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019223:Densovirinae / Q000276:immunology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005880:Gills; D008969:Molecular Sequence Data; D033541:Palaemonidae / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D054464:Peroxiredoxins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D011994:Recombinant Proteins; D054464:Peroxiredoxins