title: [Differential expression of proteins in rat mesenchymal stem cells undergoing endothelial differentiation].
abstract: OBJECTIVE
  To screen and identify differentially expressed proteins of mesenchymal stem cells (MSC) during endothelial differentiation.
  
  
  METHODS
  MSCs were induced to endothelial differentiation with vascular endothelial growth factor (VEGF) and epithelial growth factor (EGF) mixture. The whole cell proteins were extracted and isolated by two-dimensional gel electrophoresis. After gel was analyzed by Imagemaster 5.0 software, differentially expressed proteins were partially selected and identified by MALDI-TOF-MS.
  
  
  RESULTS
  The differentiated MSC highly expressed endothelial cells related markers, CD31, CD34 and FVIIIAg were 56.8%, 38.8% and 14.5% respectively by flow cytometer. Compared with the primary cultured MSC, the differentiated cells differentially expressed 91 proteins. Among the 19 identified proteins, 11 up-regulated and 8 down-regulated, which include cytoskeletal proteins, such as myosin, filamin, vimentin and vinculin; cell metabolism enzymes, such as ORP-150, ERO1-α, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, protein disulfide-isomerase A3, FAS and enolase 3; nuclear factors, such as TAR DNA binding protein, guanine nucleotide binding protein and hypoxia up-regulated protein 1; VEGF receptors, such as KDR and so on.
  
  
  CONCLUSIONS
  Cytoskeletal proteins, metabolism enzymes and KDR were all involved in endothelial differentiation of MSC. These proteins may be the regulatory targets for endothelial differentiation of MSC.
authors.affiliation: Department of Pathology and Pathophysiology, Zhejiang Medical College, Hangzhou, China.
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Liu
authors.forename: Li-qiong
authors.initials: LQ
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Rong
authors.initials: R
authors.lastname: Qiu
authors.forename: Yu-lin
authors.initials: YL
authors.lastname: Li
authors.forename: Yue-zeng
authors.initials: YZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D020543:Proteome