title: Arachidonate-enriched triglyceride oil does not promote tumor development in a rat medium-term multi-organ carcinogenesis model.
abstract: The modifying potential on tumor development of arachidonate-enriched triglyceride oil (ARA-oil) containing approximately 40% arachidonic acid was investigated in a medium-term multi-organ carcinogenesis bioassay using male and female F344 rats. The animals were sequentially given five carcinogens with different target sites in the first 4 weeks, and then administered ARA-oil for 24 weeks at dietary levels of 0% (control), 1.25%, 2.5% or 5.0%. No statistically significant differences in incidences and multiplicities of hyperplastic and neoplastic lesions were showed in the large intestine in either sex. In the liver, kidney, and lung in both sexes, and the mammary gland and uterus in females, tumor promoting potential was not evident with ARA-oil treatment. ARA-oil did not affect the quantitative data for glutathione S-transferase placental form positive foci of the liver. Increased induction of hyperplastic or neoplastic lesions in the urinary bladder and thyroid in ARA-oil-treated groups was without dose dependence. In addition, a second experiment with ARA-oil only administration for 8-week revealed no effects on cellular proliferation in the urinary bladder or thyroid in either sex. These results indicate that ARA-oil has no tumor promoting potential in any organs or tissues initiated with the five carcinogens applied in the present study.
authors.affiliation: DIMS Institute of Medical Science Inc., 64 Goura, Nishiazai, Azai-cho, Ichinomiya, Aichi 491-0113, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Imai
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kawabe
authors.forename: Seiko
authors.initials: S
authors.lastname: Tamano
authors.forename: Yuko
authors.initials: Y
authors.lastname: Doi
authors.forename: Hironao
authors.initials: H
authors.lastname: Nakashima
authors.forename: Mayuko
authors.initials: M
authors.lastname: Suguro
authors.forename: Takamasa
authors.initials: T
authors.lastname: Numano
authors.forename: Tomomi
authors.initials: T
authors.lastname: Hara
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hagiwara
authors.forename: Fumio
authors.initials: F
authors.lastname: Furukawa
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Kaneda
authors.forename: Norifumi
authors.initials: N
authors.lastname: Tateishi
authors.forename: Wataru
authors.initials: W
authors.lastname: Fujii
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawashima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shibata
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sakakibara
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000008:administration & dosage*; D001835:Body Weight; D002471:Cell Transformation, Neoplastic; D004327:Drinking Behavior; D005247:Feeding Behavior; D005260:Female; D008297:Male; D009374:Neoplasms, Experimental / Q000473:pathology*; D009929:Organ Size; D051381:Rats; D011916:Rats, Inbred F344; D014280:Triglycerides / Q000008:administration & dosage*
chemical_list: D014280:Triglycerides; D016718:Arachidonic Acid