title: Direct evidence that HSV DNA damaged by ultraviolet (UV) irradiation can be repaired in a cell type-dependent manner.
abstract: Infection of permissive cells, in tissue culture, with herpes simplex virus (HSV) has been reported to induce host DNA damage repair responses that are necessary for efficient viral replication. However, direct repair of the damaged viral DNA has not, to our knowledge, been shown. In this report, we detect and determine the amount of damaged HSV-1 DNA, following introduction of experimentally damaged HSV genomes into tissue cultures of permissive Vero, NGF differentiated PC12 cells and primary rat neurons, using a method of detection introduced here. The results show that HSV-1 strain 17 DNA containing UV-induced DNA damage is efficiently repaired, in Vero, but not NGF differentiated PC12 cells. The primary rat neuronal cultures were capable of repairing the damaged viral DNA, but with much less efficiency than did the permissive Vero cells. Moreover, by conducting the experiments with either an inhibitor of the HSV polymerase (phosphonoacetic acid [PAA]) or with a replication defective DNA polymerase mutant virus, HP66, the results suggest that repair can occur in the absence of a functional viral polymerase, although polymerase function seems to enhance the efficiency of the repair, in a replication independent manner. The possible significance of varying cell type mediated repair of viral DNA to viral pathogenesis is discussed.
authors.affiliation: Drexel Institute for Biotechnology and Virology Research and Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, USA. scott.millhouse@ihvr.org
authors.forename: Scott
authors.initials: S
authors.lastname: Millhouse
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Wang
authors.forename: Nigel W
authors.initials: NW
authors.lastname: Fraser
authors.forename: Lisa
authors.initials: L
authors.lastname: Faber
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Block
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004249:DNA Damage; D004260:DNA Repair; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006561:Herpes Simplex / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D058507:Host Specificity; D054884:Host-Pathogen Interactions; D009474:Neurons / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D019384:Nucleic Acid Synthesis Inhibitors; D016716:PC12 Cells; D010746:Phosphonoacetic Acid / Q000494:pharmacology; D061251:Primary Cell Culture; D051381:Rats; D018139:Simplexvirus / Q000235:genetics* / Q000378:metabolism; D014466:Ultraviolet Rays; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D004791:Enzyme Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D004259:DNA-Directed DNA Polymerase; D010746:Phosphonoacetic Acid