title: Primary style protein expression in the self-incompatible/compatible apricot by the 2D-DIGE technique.
abstract: In order to explore the molecular mechanism underlying self-incompatibility (SI) in the apricot (Prunus armeniaca L.) at the proteome level, we examined the style proteomes at different stages of flower development: small bud, big bud, 24h after self-pollination and 24h after cross-pollination with cultivar Badanshui in the SI apricot cultivar Xinshiji and the self-compatible (SC) apricot cultivar Katy by 2D fluorescence difference gel electrophoresis (2D-DIGE) and mass spectrometry (MS). About 1500 style protein spots were detected; 66 were expressed differently in the four stages in Xinshiji. About 1600 style protein spots were detected; 143 were expressed differently in the four stages of flower development in Katy. In Xinshiji, one protein was expressed specifically, four proteins showed up-regulated expression and twenty-nine proteins showed down-regulated expression in the cross-pollinated style compared to the self-pollinated style. Thirteen proteins were identified unambiguously. In Katy, three proteins were expressed specifically, five proteins showed up-regulated expression and thirteen proteins showed down-regulated expression in the cross-pollinated style compared to self-pollinated style. Seven proteins were identified unambiguously. The different reactions of the style at the proteomic level were triggered in Xinshiji and Katy by self pollen and non-self pollen.
authors.affiliation: Horticultural Department of Shihezi University, Shihezi, Xinjiang, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Cao
authors.forename: Jianrong
authors.initials: J
authors.lastname: Feng
authors.forename: Dajiang
authors.initials: D
authors.lastname: Wang
authors.forename: Junli
authors.initials: J
authors.lastname: Sun
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Lu
authors.forename: Huaifeng
authors.initials: H
authors.lastname: Liu
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D054817:Pollination / Q000235:genetics; D020543:Proteome / Q000235:genetics; D027861:Prunus / Q000235:genetics*; D060436:Self-Incompatibility in Flowering Plants / Q000235:genetics*; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*
chemical_list: D010940:Plant Proteins; D020543:Proteome