title: Evaluation of hair roots for detection of methamphetamine and 3,4-methylenedioxymethamphetamine abuse by use of an HPLC-chemiluminescence method.
abstract: We describe the use of hair roots as a matrix for detection of methamphetamine (MP) and 3,4-methylenedioxymethamphetamine (MDMA) abuse. The concentration of drugs was determined in rat hair roots, hair shafts, and plasma after a single administration of MP or MDMA, by use of an HPLC-peroxyoxalate chemiluminescence (PO-CL) method involving column switching. Plasma and hair roots and shafts were collected from male Wistar rats before and after administration of MP (10 mg kg(-1), i.p.). In addition, the roots and shafts of pigmented and non-pigmented hair of male Lister hooded rats were collected after administration of MDMA (10 mg kg(-1), i.p.). The concentrations of MP and MDMA in plasma and hair were determined by use of the HPLC-PO-CL method, with satisfactory sensitivity and reproducibility. The concentration of MP in hair roots 1-14 days after administration ranged from 0.038 to 0.115 ng mg(-1) (n = 3). By use of the HPLC-PO-CL method, MP could be detected in hair roots for longer (up to 14 days) than it could be detected in conventional biological specimens, for example plasma (~1 day), and MDMA was detected in hair roots from 1 to 10 days after administration. The AUC(1-10) (ng day mg(-1)) for MDMA in roots of non-pigmented and pigmented hair was comparable (4.93 ± 2.09 vs. 6.67 ± 1.28, n = 3), whereas AUC(1-14) for hair shafts differed significantly (1.86 ± 0.93 vs. 4.58 ± 0.63, P < 0.05, n = 3). The window for detecting MP (or MDMA) in hair roots under our conditions was 1-14 (or 1-10) days.
authors.affiliation: Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan. m-wada@nagasaki-u.ac.jp
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Wada
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ochi
authors.forename: Kumi
authors.initials: K
authors.lastname: Nogami
authors.forename: Rie
authors.initials: R
authors.lastname: Ikeda
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D000697:Central Nervous System Stimulants / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006197:Hair / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008297:Male; D008694:Methamphetamine / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000295:instrumentation / Q000379:methods*
chemical_list: D000697:Central Nervous System Stimulants; D008694:Methamphetamine; D018817:N-Methyl-3,4-methylenedioxyamphetamine