title: High-resolution melting analysis for rapid detection of linezolid resistance (mediated by G2576T mutation) in Staphylococcus epidermidis.
abstract: We describe a novel HRM-PCR (high-resolution melting) assay capable of the accurate identification of the G2576T point mutation in domain V of the 23S rRNA genes attributed to linezolid resistance in Staphylococcus epidermidis. This rapid method demonstrated 100% correlation with the previously established NheI restriction digest assay.
authors.affiliation: Cork Institute of Technology, Cork, Ireland.
authors.forename: Emma M
authors.initials: EM
authors.lastname: Gabriel
authors.forename: Pierre E
authors.initials: PE
authors.lastname: Douarre
authors.forename: Siobhan
authors.initials: S
authors.lastname: Fitzgibbon
authors.forename: James
authors.initials: J
authors.lastname: Clair
authors.forename: Brigid
authors.initials: B
authors.lastname: Lucey
authors.forename: Aidan
authors.initials: A
authors.lastname: Coffey
authors.forename: Jim M
authors.initials: JM
authors.lastname: O'Mahony
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D024881:Drug Resistance, Bacterial; D006801:Humans; D000069349:Linezolid; D008826:Microbial Sensitivity Tests / Q000379:methods; D023303:Oxazolidinones / Q000494:pharmacology*; D017354:Point Mutation; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000235:genetics*; D044366:Transition Temperature
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D023303:Oxazolidinones; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D000069349:Linezolid