title: Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis.
abstract: Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K+ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.
authors.affiliation: Laboratory of Plant Physiology and Biophysics, Institute of Molecular, Cell and Systems Biology, University of Glasgow, Glasgow, G12 8QQ, UK. Michael.Blatt@glasgow.ac.uk.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Chen
authors.forename: Cornelia
authors.initials: C
authors.lastname: Eisenach
authors.forename: Xin-Qin
authors.initials: XQ
authors.lastname: Xu
authors.forename: Adrian
authors.initials: A
authors.lastname: Hills
authors.forename: Michael R
authors.initials: MR
authors.lastname: Blatt