title: Down-regulation of heat-shock protein 27-induced resistance to photodynamic therapy in oral cancer cells.
abstract: Photodynamic therapy (PDT) of cells is a new treatment modality involving selective delivery of a photosensitive dye into target cells, followed by visible light irradiation. PDT induces cell death by excessive ROS generation. The effects of multiple photosensitizers were owing to the difference in cell types involving sensitizer-specific protein changes linked to resistance. HSP27 is regulated in response to stress and is associated with apoptotic process. The effects of HSP27 on PDT resistance are controversial and unclear. The purpose of this study was to investigate the role of HSP27 down-regulation in the PDT-induced cells and HSP27 regulation in the resistance to PDT. KB cells transfected with HSP27 siRNA were exposed to hematoporphyrin (HP) followed by irradiation at 635 nm at an energy density of 4.5 mW/cm(2). After irradiation, the effects on HSP27 down-regulation were assessed by MTT assay, flow cytometry, confocal analysis, Western blotting and caspase activity. The results of this study showed that down-regulation of HSP27 restored cell survival in HP-PDT-induced apoptotic KB cells. HSP27 down-regulation attenuated PDT-induced apoptosis through caspase-mediated pathway in KB cells. Also, HSP27 silencing regulated Bax, Bcl-2, and PARP protein expression in PDT-induced cells. Therefore, HSP27 down-regulation confers resistance to PDT through interruption of apoptotic protein activity in PDT-induced cell death. HSP27 might contribute to regulating PDT-induced apoptosis in PDT-resistant cells.
authors.affiliation: Department of Oral Pathology, 2nd stage of Brain Korea 21 for School of Dentistry, Dental Science Research Institute, Chonnam National University, Bug-Gu, Gwangju, Korea.
authors.forename: Jisun
authors.initials: J
authors.lastname: Kim
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Jung
authors.forename: Wonbong
authors.initials: W
authors.lastname: Lim
authors.forename: Sangwoo
authors.initials: S
authors.lastname: Kim
authors.forename: Youngjong
authors.initials: Y
authors.lastname: Ko
authors.forename: Sandeep
authors.initials: S
authors.lastname: Karna
authors.forename: Oksu
authors.initials: O
authors.lastname: Kim
authors.forename: Yooduk
authors.initials: Y
authors.lastname: Choi
authors.forename: Hongran
authors.initials: H
authors.lastname: Choi
authors.forename: Okjoon
authors.initials: O
authors.lastname: Kim
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D020868:Gene Silencing; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000502:physiology; D006801:Humans; D007624:KB Cells; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics; D010778:Photochemotherapy; D034741:RNA, Small Interfering
chemical_list: D055551:HSP27 Heat-Shock Proteins; D034741:RNA, Small Interfering; D020169:Caspases