title: Resistance to linezolid in a methicillin-susceptible Staphylococcus aureus clinical isolate without previous exposure to oxazolidinones.
abstract: A linezolid-resistant, methicillin-susceptible isolate of Staphylococcus aureus was obtained from an infected surgical wound in an ambulatory patient. The isolate belonged to ST125 and was susceptible to all the antibiotics tested except linezolid (MIC 8 μg/ml) and levofloxacin. Linezolid resistance could be ascribed to the presence of the mutation G2576T in 2 of the 23S rRNA alleles. The mutant alleles were stably maintained in the absence of antibiotic selection.
authors.affiliation: Servicio de Microbiología, Hospital Universitario La Paz. IdiPAZ, Paseo de La Castellana, 261, Madrid 28046, Spain.
authors.forename: Immaculada
authors.initials: I
authors.lastname: Quiles-Melero
authors.forename: Adela
authors.initials: A
authors.lastname: García-Perea
authors.forename: Manuela
authors.initials: M
authors.lastname: de Pablos
authors.forename: Rosa
authors.initials: R
authors.lastname: Gómez-Gil
authors.forename: Jesús
authors.initials: J
authors.lastname: Mingorance
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D000069349:Linezolid; D008712:Methicillin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010045:Outpatients; D023303:Oxazolidinones / Q000494:pharmacology* / Q000627:therapeutic use; D017354:Point Mutation; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D013530:Surgical Wound Infection / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D023303:Oxazolidinones; D012338:RNA, Ribosomal, 23S; D000069349:Linezolid; D008712:Methicillin