title: Molecular epidemiology of Enterococcus sp. isolated in a university hospital in Algeria.
abstract: BACKGROUND
  The aim of this study was to describe the epidemiology of enterococci isolated from infections at an Algerian university hospital, and to evaluate the prevalence of vancomycin-resistant enterococci (VRE) and the clonal cluster present in this country.
  
  
  METHODS
  Patients who presented at Annaba University Hospital with Enterococcus infections were prospectively included over a 1-y period (2010). All Enterococcus sp. isolated were characterized by antibiotic resistance, van and erm genes, repetitive sequence-based polymerase chain reaction (rep-PCR), multi-locus sequence typing (MLST), and virulence genes.
  
  
  RESULTS
  A total of 125 Enterococcus isolates recovered from 125 patients (59% female; median age 54 y, range 2-86 y) were studied. No differences in epidemiological data were observed between infections by Enterococcus faecalis vs Enterococcus faecium. However a high proportion of E. faecium were resistant to ampicillin (95%). The prevalence of VRE, corresponding to 4 vanC1-Enterococcus gallinarum, was 3.2%. A high level of genomic diversity among strains was noted, with the importance of sequence type (ST) 78 (which belongs to clonal complex (CC) 17) in E. faecium and ST317 and CC2 in E. faecalis.
  
  
  CONCLUSIONS
  This first study on enterococci isolated in Algeria shows the low prevalence of VRE, but the presence of clonal complexes linked to VRE and vancomycin-sensitive enterococci associated with hospital infections. Moreover the high level of macrolide resistance and/or ampicillin resistance in E. faecium suggests close monitoring of the epidemiology of these strains.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Université Montpellier 1, UFR de Médecine, Nîmes, France.
authors.forename: Nassima
authors.initials: N
authors.lastname: Djahmi
authors.forename: Adeline
authors.initials: A
authors.lastname: Boutet-Dubois
authors.forename: Sabrina
authors.initials: S
authors.lastname: Nedjai
authors.forename: Mazouz
authors.initials: M
authors.lastname: Dekhil
authors.forename: Albert
authors.initials: A
authors.lastname: Sotto
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Lavigne
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000462:Algeria / Q000453:epidemiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology*; D006785:Hospitals, University; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D015995:Prevalence; D020713:Vancomycin Resistance; D014774:Virulence / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents