title: Differential patterns of synaptotagmin7 mRNA expression in rats with kainate- and pilocarpine-induced seizures.
abstract: Previous studies in rat models of neurodegenerative disorders have shown disregulation of striatal synaptotagmin7 mRNA. Here we explored the expression of synaptotagmin7 mRNA in the brains of rats with seizures triggered by the glutamatergic agonist kainate (10 mg/kg) or by the muscarinic agonist pilocarpine (30 mg/kg) in LiCl (3 mEq/kg) pre-treated (24 h) rats, in a time-course experiment (30 min-1 day). After kainate-induced seizures, synaptotagmin7 mRNA levels were transiently and uniformly increased throughout the dorsal and ventral striatum (accumbens) at 8 and 12 h, but not at 24 h, followed at 24 h by somewhat variable upregulation within different parts of the cerebral cortex, amigdala and thalamic nuclei, the hippocampus and the lateral septum. By contrast, after LiCl/pilocarpine-induced seizures, there was a more prolonged increase of striatal Synaptotagmin7 mRNA levels (at 8, 12 and 24 h), but only in the ventromedial striatum, while in some other of the aforementioned brain regions there was a decline to below the basal levels. After systemic post-treatment with muscarinic antagonist scopolamine in a dose of 2 mg/kg the seizures were either extinguished or attenuated. In scopolamine post-treated animals with extinguished seizures the striatal synaptotagmin7 mRNA levels (at 12 h after the onset of seizures) were not different from the levels in control animals without seizures, while in rats with attenuated seizures, the upregulation closely resembled kainate seizures-like pattern of striatal upregulation. In the dose of 1 mg/kg, scopolamine did not significantly affect the progression of pilocarpine-induced seizures or pilocarpine seizures-like pattern of striatal upregulation of synaptotagmin7 mRNA. In control experiments, equivalent doses of scopolamine per se did not affect the expression of synaptotagmin7 mRNA. We conclude that here described differential time course and pattern of synaptotagmin7 mRNA expression imply regional differences of pathophysiological brain activation and plasticity in these two models of seizures.
authors.affiliation: Brain Research Laboratory, Institute of Pathophysiology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Gordana
authors.initials: G
authors.lastname: Glavan
authors.forename: Ronald Eugene
authors.initials: RE
authors.lastname: See
authors.forename: Marko
authors.initials: M
authors.lastname: Živin
mesh_terms: D000818:Animals; D007608:Kainic Acid / Q000009:adverse effects*; D008297:Male; D018721:Muscarinic Agonists / Q000009:adverse effects*; D018727:Muscarinic Antagonists / Q000009:adverse effects*; D010862:Pilocarpine / Q000009:adverse effects*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced* / Q000235:genetics*; D050857:Synaptotagmins / Q000235:genetics*
chemical_list: D018721:Muscarinic Agonists; D018727:Muscarinic Antagonists; D012333:RNA, Messenger; D010862:Pilocarpine; D050857:Synaptotagmins; D007608:Kainic Acid