title: Immunogenic analysis of two DNA vaccines of avian reovirus mediated by attenuated Salmonella typhimurium in chickens.
abstract: Avian reovirus (ARV) is an important pathogen in poultry industry and causes great economic losses. As attenuated Salmonella typhimurium is already being used as an effective vehicle for the transfer of DNA vaccines, so in this study we evaluated two DNA vaccines mediated by S. typhimurium on their ability of eliciting antibody production. SPF chickens were respectively immunized with SL7207 (pVAX-σB), SL7207 (pVAX-σC) and SL7027 (pVAX-σB-σC) three times. The results showed that the antibody production was highly dependent on the immunizing times, detectable antibodies of serum antibody IgG and small intestinal mucosal antibody IgA were generated at week 4 and were further improved at week 6 and antibody titers in group SL7207 (pVAX-σC) were higher than that in group SL7207 (pVAX-σB), demonstrating that SL7207 (pVAX-σC) was more powerful than SL7207 (pVAX-σB) in antibody production. The higher antibody titer in SL7027 (pVAX-σB-σC) than that in SL7207 (pVAX-σC) group showed that co-expressing σB and σC could improve antibody production. IFN-γ detection showed that significant higher IFN-γ was generated both in groups SL7027 (pVAX-σB-σC) and SL7207 (pVAX-σC). Subsequent challenge showed that SL7207 (pVAX-σB), SL7207 (pVAX-σC) and SL7027 (pVAX-σB-σC) conferred 50%, 75% and 87.5% respectively.
authors.affiliation: Laboratory of Animal Infectious Disease and Microarray, Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Wan
authors.forename: Xintian
authors.initials: X
authors.lastname: Wen
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Qigui
authors.initials: Q
authors.lastname: Yan
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhao
authors.forename: Sanjie
authors.initials: S
authors.lastname: Cao
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001483:Base Sequence; D001715:Bird Diseases / Q000276:immunology / Q000517:prevention & control*; D002645:Chickens / Q000276:immunology* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D018928:Immunity, Mucosal; D007371:Interferon-gamma / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000276:immunology; D030101:Orthoreovirus, Avian / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D012088:Reoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014765:Viral Vaccines; D007371:Interferon-gamma