title: Selected telomere length changes and aberrant three-dimensional nuclear telomere organization during fast-onset mouse plasmacytomas.
abstract: Mouse plasmacytoma (PCT) can develop within 45 days when induced by a v-abl/myc replication-deficient retrovirus. This fast-onset PCT development is always associated with trisomy of cytoband E2 of mouse chromosome 11 (11E2). Trisomy of 11E2 was identified as the sole aberration in all fast-onset mouse PCTs in [T38HxBALB/c]N congenic mice, with a reciprocal translocation between chromosome X and 11 (rcpT(X;11)) (Genes Cancer 2010;1:847-858). Using this mouse model, we have now examined the overall and individual telomere lengths in fast-onset PCTs compared with normal B cells using two-dimensional and three-dimensional quantitative fluorescent in situ hybridization of telomeres. We found fast-onset PCTs to have a significantly different three-dimensional telomere profile, compared with primary B cells of wild-type littermates with and without rcpT(X;11) (P < .0001 and P = .006, respectively). Our data also indicate for primary PCT cells, from the above mouse strain, that the translocation chromosome carrying 11E2 is the only chromosome with telomere lengthening (P = 4 x 10(-16)). This trend is not seen for T(X;11) in primary B cells of control [T38HxBALB/c]N mice with the rcpT(X;11). This finding supports the concept of individual telomere lengthening of chromosomes that are functionally important for the tumorigenic process.
authors.affiliation: Manitoba Institute of Cell Biology, The University of Manitoba, Cancer Care Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kuzyk
authors.forename: Sabine
authors.initials: S
authors.lastname: Mai
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics* / Q000648:ultrastructure; D004195:Disease Models, Animal; D021621:Imaging, Three-Dimensional; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma / Q000235:genetics* / Q000648:ultrastructure*; D016615:Telomere / Q000235:genetics / Q000473:pathology*; D059505:Telomere Homeostasis / Q000235:genetics*; D014178:Translocation, Genetic