title: Construction of shRNA lentiviral vector.
abstract: Lentiviruses have been adapted as gene delivery vehicles. This article summarized shRNA lentiviral vector methods generally used in research laboratories. The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, (3) using packaging cells to produce shRNA lentivirus, and (4) transducing target cells with shRNA lentivirus.
authors.affiliation: Department of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: Hong
authors.initials: H
authors.lastname: Song
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Yang