title: A single polar residue and distinct membrane topologies impact the function of the infectious bronchitis coronavirus E protein.
abstract: The coronavirus E protein is a small membrane protein with a single predicted hydrophobic domain (HD), and has a poorly defined role in infection. The E protein is thought to promote virion assembly, which occurs in the Golgi region of infected cells. It has also been implicated in the release of infectious particles after budding. The E protein has ion channel activity in vitro, although a role for channel activity in infection has not been established. Furthermore, the membrane topology of the E protein is of considerable debate, and the protein may adopt more than one topology during infection. We previously showed that the HD of the infectious bronchitis virus (IBV) E protein is required for the efficient release of infectious virus, an activity that correlated with disruption of the secretory pathway. Here we report that a single residue within the hydrophobic domain, Thr16, is required for secretory pathway disruption. Substitutions of other residues for Thr16 were not tolerated. Mutations of Thr16 did not impact virus assembly as judged by virus-like particle production, suggesting that alteration of secretory pathway and assembly are independent activities. We also examined how the membrane topology of IBV E affected its function by generating mutant versions that adopted either a transmembrane or membrane hairpin topology. We found that a transmembrane topology was required for disrupting the secretory pathway, but was less efficient for virus-like particle production. The hairpin version of E was unable to disrupt the secretory pathway or produce particles. The findings reported here identify properties of the E protein that are important for its function, and provide insight into how the E protein may perform multiple roles during infection.
authors.affiliation: Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Travis R
authors.initials: TR
authors.lastname: Ruch
authors.forename: Carolyn E
authors.initials: CE
authors.lastname: Machamer
mesh_terms: D000595:Amino Acid Sequence; D001692:Biological Transport; D045744:Cell Line, Tumor; D018352:Coronavirus Infections / Q000378:metabolism / Q000821:virology*; D006056:Golgi Apparatus / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D001351:Infectious bronchitis virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D009154:Mutation; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D055571:Secretory Pathway; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism*; D019065:Virus Assembly
chemical_list: D014759:Viral Envelope Proteins