title: A basic consideration for porcine liver preservation using a novel continuous machine perfusion device.
abstract: BACKGROUND
  The aims of this study were to compare extracellular and intracellular-type University of Wisconsin (UW) solutions for liver grafts and to assess oxygenation in this perfusion system.
  
  
  METHODS
  The organ preservation system consisted of 3 circulating systems for the portal vein, hepatic artery, and maintenance of the perfusion solution. The portal vein or hepatic artery system had a roller pump, a flow meter, and a pressure sensor. In this study, we perfused livers with UW or extracellular type UW-gluconate at 4°C-6°C for 4 hours. The flow rates at the entrance were 0.5 mL/min/g liver in the portal vein and 0.2 mL/min/liver in the hepatic artery. Orthotopic liver transplantation was performed in pigs: group 1-a, grafts procured after acute hemorrhagic shock were preserved by a solution without O(2); group 1-b, grafts were preserved with O(2); group 2-a, grafts were perfused using intracellular type solution (UW); and group 2-b, grafts were perfused using extracellular-type solution (UW-gluconate).
  
  
  RESULTS
  Effluent aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) levels in group 1-b were lower than those in group 1-a. Survival rates in group 2-a and group 2-b were 1/4 and 3/3, respectively. Effluent AST and LDH levels in the perfusate of group 2-b were lower than group 2-a. Histological study revealed necrosis of hepatocytes and sinusoidal congestion in group 2-a.
  
  
  CONCLUSIONS
  A beneficial effect of extracellular-type solution with oxygenation in a novel continuous machine preservation system yielded well-preserved liver graft function.
authors.affiliation: Transplantation and Innovative Surgery, National Center for Child Health and Development, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shigeta
authors.forename: N
authors.initials: N
authors.lastname: Matsuno
authors.forename: H
authors.initials: H
authors.lastname: Huai-Che
authors.forename: H
authors.initials: H
authors.lastname: Obara
authors.forename: H
authors.initials: H
authors.lastname: Mizunuma
authors.forename: T
authors.initials: T
authors.lastname: Hirano
authors.forename: S
authors.initials: S
authors.lastname: Uemoto
authors.forename: S
authors.initials: S
authors.lastname: Enosawa
mesh_terms: D000241:Adenosine / Q000008:administration & dosage; D000493:Allopurinol / Q000008:administration & dosage; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D003080:Cold Temperature; D004867:Equipment Design; D005942:Gluconates / Q000008:administration & dosage*; D005978:Glutathione / Q000008:administration & dosage; D006499:Hepatic Artery / Q000601:surgery*; D007328:Insulin / Q000008:administration & dosage; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000098:blood supply / Q000201:enzymology / Q000473:pathology / Q000601:surgery*; D016031:Liver Transplantation; D009336:Necrosis; D009926:Organ Preservation / Q000295:instrumentation*; D019188:Organ Preservation Solutions / Q000008:administration & dosage*; D010100:Oxygen / Q000008:administration & dosage*; D010477:Perfusion / Q000295:instrumentation*; D011169:Portal Vein / Q000601:surgery*; D011887:Raffinose / Q000008:administration & dosage; D034421:Sus scrofa; D013997:Time Factors
chemical_list: D005942:Gluconates; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; C030691:gluconic acid; D010100:Oxygen