title: Increased levels of conditioned fear and avoidance behavior coincide with changes in phosphorylation of the protein kinase B (AKT) within the amygdala in a mouse model of extremes in trait anxiety.
abstract: Patients diagnosed for anxiety disorders often display faster acquisition and slower extinction of learned fear. To gain further insights into the mechanisms underlying these phenomenona, we studied conditioned fear in mice originating form a bi-directional selective breeding approach, which is based on elevated plus-maze behavior and results in CD1-derived high (HAB), normal (NAB), and low (LAB) anxiety-related behavior mice. HAB mice displayed pronounced cued-conditioned fear compared to NAB/CD1 and LAB mice that coincided with increased phosphorylation of the protein kinase B (AKT) in the basolateral amygdala 45 min after conditioning. No similar changes were observed after non-associative immediate shock presentations. Fear extinction of recent but not older fear memories was preserved. However, HAB mice were more prone to relapse of conditioned fear with the passage of time. HAB mice also displayed higher levels of contextual fear compared to NAB and LAB mice and exaggerated avoidance following step-down avoidance training. Interestingly, HAB mice showed lower and LAB mice higher levels of acoustic startle responses compared to NAB controls. The increase in arousal observed in LAB mice coincided with the general absence of conditioned freezing. Taken together, our results suggest that the genetic predisposition to high anxiety-related behavior may increase the risk of forming traumatic memories, phobic-like fear and avoidance behavior following aversive encounters, with a clear bias towards passive coping styles. In contrast, genetic predisposition to low anxiety-related and high risk-taking behavior seems to be associated with an increase in active coping styles. Our data imply changes in AKT phosphorylation as a therapeutic target for the prevention of exaggerated fear memories.
authors.affiliation: Max Planck Institute of Psychiatry, Kraepelinstr. 2, D-80804 Munich, Germany. yen@mpipsykl.mpg.de
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Yen
authors.forename: Christoph P
authors.initials: CP
authors.lastname: Mauch
authors.forename: Maik
authors.initials: M
authors.lastname: Dahlhoff
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Micale
authors.forename: Mirjam
authors.initials: M
authors.lastname: Bunck
authors.forename: Simone B
authors.initials: SB
authors.lastname: Sartori
authors.forename: Nicolas
authors.initials: N
authors.lastname: Singewald
authors.forename: Rainer
authors.initials: R
authors.lastname: Landgraf
authors.forename: Carsten T
authors.initials: CT
authors.lastname: Wotjak
mesh_terms: D000161:Acoustic Stimulation; D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D001007:Anxiety / Q000378:metabolism* / Q000523:psychology; D001362:Avoidance Learning / Q000502:physiology*; D001522:Behavior, Animal / Q000502:physiology*; D003213:Conditioning, Psychological / Q000502:physiology*; D003463:Cues; D004195:Disease Models, Animal; D004597:Electroshock; D005108:Extinction, Psychological / Q000502:physiology; D005239:Fear / Q000502:physiology*; D008297:Male; D051379:Mice; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*
chemical_list: D051057:Proto-Oncogene Proteins c-akt