title: Real-time monitoring and control of microbial bioprocesses with focus on the specific growth rate: current state and perspectives.
abstract: Understanding the growth characteristics of microorganisms is an essential step in bioprocessing, not only because product formation may be growth-associated but also because they might influence cell physiology and thereby product quality. The specific growth rate, a key variable of many bioprocesses, cannot be measured directly and relies on the estimation through other measurable variables such as biomass, substrate, or product concentrations. Techniques for real-time estimation of the specific growth rate in microbial fed-batch cultures are discussed in the present paper. The advantages and limitations of different models and various monitoring techniques are discussed, highlighting the importance of the specific growth rate in the development of fast, reliable, and robust processes for the production of high-value products such as recombinant proteins.
authors.affiliation: Laboratory of Integrated Bioprocessing, Dublin City University, Dublin 9, Ireland. moira.schuler@dcu.ie
authors.forename: Moira Monika
authors.initials: MM
authors.lastname: Schuler
authors.forename: Ian William
authors.initials: IW
authors.lastname: Marison
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism; D061249:Batch Cell Culture Techniques / Q000379:methods / Q000639:trends; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media; D007700:Kinetics; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins