title: Age-associated declines in mitochondrial biogenesis and protein quality control factors are minimized by exercise training.
abstract: A decline in mitochondrial biogenesis and mitochondrial protein quality control in skeletal muscle is a common finding in aging, but exercise training has been suggested as a possible cure. In this report, we tested the hypothesis that moderate-intensity exercise training could prevent the age-associated deterioration in mitochondrial biogenesis in the gastrocnemius muscle of Wistar rats. Exercise training, consisting of treadmill running at 60% of the initial Vo(2max), reversed or attenuated significant age-associated (detrimental) declines in mitochondrial mass (succinate dehydrogenase, citrate synthase, cytochrome-c oxidase-4, mtDNA), SIRT1 activity, AMPK, pAMPK, and peroxisome proliferator-activated receptor gamma coactivator 1-α, UCP3, and the Lon protease. Exercise training also decreased the gap between young and old animals in other measured parameters, including nuclear respiratory factor 1, mitochondrial transcription factor A, fission-1, mitofusin-1, and polynucleotide phosphorylase levels. We conclude that exercise training can help minimize detrimental skeletal muscle aging deficits by improving mitochondrial protein quality control and biogenesis.
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
authors.forename: Erika
authors.initials: E
authors.lastname: Koltai
authors.forename: Nikolett
authors.initials: N
authors.lastname: Hart
authors.forename: Albert W
authors.initials: AW
authors.lastname: Taylor
authors.forename: Sataro
authors.initials: S
authors.lastname: Goto
authors.forename: Jenny K
authors.initials: JK
authors.lastname: Ngo
authors.forename: Kelvin J A
authors.initials: KJ
authors.lastname: Davies
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Radak
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000375:Aging / Q000378:metabolism*; D000818:Animals; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000378:metabolism*; D051266:Nuclear Respiratory Factor 1 / Q000378:metabolism; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C503403:Fis1 protein, rat; D008565:Membrane Proteins; C489709:Mfn1 protein, rat; D024101:Mitochondrial Proteins; D051266:Nuclear Respiratory Factor 1; C489030:Tfam protein, rat; D014157:Transcription Factors