title: Structural analysis of the O-polysaccharide of the lipopolysaccharide from Azospirillum brasilense Jm6B2 containing 3-O-methyl-D-rhamnose (D-acofriose).
abstract: Two types of neutral O-polysaccharides were obtained by mild acid degradation of the lipopolysaccharide isolated by phenol-water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense Jm6B2. The following structure of the major O-polysaccharide was established by composition and methylation (ethylation) analyses, Smith degradation, and 1D and 2D (1)H and (13)C NMR spectroscopy: [structure: see text] where a non-stoichiometric (~60%) 3-O-methylation of D-rhamnose is indicated by italics.
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia.
authors.forename: Alevtina S
authors.initials: AS
authors.lastname: Boyko
authors.forename: Andrey S
authors.initials: AS
authors.lastname: Dmitrenok
authors.forename: Yuliya P
authors.initials: YP
authors.lastname: Fedonenko
authors.forename: Evelina L
authors.initials: EL
authors.lastname: Zdorovenko
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Konnova
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Ignatov
mesh_terms: D015806:Azospirillum brasilense / Q000737:chemistry* / Q000254:growth & development; D002236:Carbohydrate Conformation; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D008358:Mannose / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D008070:Lipopolysaccharides; C006396:acofriose; D008358:Mannose