title: Chromatin immunoprecipitation identifies genes under direct VraSR regulation in  Staphylococcus aureus.
abstract: Transcriptional profiling of  Staphylococcus aureus  treated with cell wall-active antibiotics identified the 2-component system, VraSR, as one of the key players in response to antibiotic stress. Although it has been shown that a number of genes are regulated by the VraSR system, it has not been shown which genes are under direct VraSR regulation and which genes are not. In this study, chromatin immunoprecipitation techniques were used to identify the genes which are regulated by the direct interaction of VraR with their promoter regions. The results showed for the first time, that the VraSR mediated regulation of cell wall biosynthesis-associated genes, pbp2, murZ, and sgtB are facilitated by the direct binding of VraR to their respective promoters. Conversely, fmtA, indicated previously to be under VraSR regulation did not exhibit direct regulation by the binding of VraR to its promoter. The VraSR system plays a very important role in antibiotic resistance against cell wall-active antibiotics, and hence, it is essential to understand its complete regulatory mechanism.
authors.affiliation: School of Biological Sciences, Illinois State University, Normal, IL 61790-4120, USA.
authors.forename: Mrittika
authors.initials: M
authors.lastname: Sengupta
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Jain
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Wilkinson
authors.forename: Radheshyam K
authors.initials: RK
authors.lastname: Jayaswal
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D006801:Humans; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism; D020713:Vancomycin Resistance / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C478170:VraR protein, Staphylococcus aureus; C478169:VraS protein, Staphylococcus aureus