title: In vivo detection of 13C isotopomer turnover in the human brain by sequential infusion of 13C labeled substrates.
abstract: This study demonstrates the feasibility of simultaneously detecting human brain metabolites labeled by two substrates infused in a sequential order. In vivo (13)C spectra of carboxylic/amide carbons were acquired only during the infusion of the second substrate. This approach allowed dynamic detection of (13)C labeling from two substrates with considerably different labeling patterns. [2-(13)C]glucose and [U-(13)C(6)]glucose were used to generate singlet and doublet signals of the same carboxylic/amide carbon atom, respectively. Because of the large one-bond (13)C-(13)C homonuclear J coupling between a carboxylic/amide carbon and an aliphatic carbon (~50 Hz), the singlet and doublet signals of the same carboxylic/amide carbon were well distinguished. The results demonstrated that different (13)C isotopomer patterns could be simultaneously and distinctly measured in vivo in a clinical setting at 3T.
authors.affiliation: Magnetic Resonance Spectroscopy Core Facility, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-1527, USA.
authors.forename: Shizhe
authors.initials: S
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Maria
authors.initials: M
authors.lastname: Ferraris Araneta
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.forename: Christopher
authors.initials: C
authors.lastname: Johnson
authors.forename: Robert B
authors.initials: RB
authors.lastname: Innis
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
mesh_terms: D000328:Adult; D000577:Amides / Q000737:chemistry / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002247:Carbon Isotopes; D002264:Carboxylic Acids / Q000737:chemistry / Q000378:metabolism; D004574:Electromagnetic Fields; D005240:Feasibility Studies; D005260:Female; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D006801:Humans; D007262:Infusions, Intravenous; D007536:Isomerism; D008297:Male; D013269:Stochastic Processes
chemical_list: D000577:Amides; D000596:Amino Acids; D002247:Carbon Isotopes; D002264:Carboxylic Acids; D005947:Glucose