title: Visible light-driven NADH regeneration sensitized by proflavine for biocatalysis.
abstract: Harvest time: Proflavine drives the reduction of NAD(+)  in the presence of a Rh-based electron mediator. Photoregenerated NADH was enzymatically active for oxidation by NADH-dependent L-glutamate dehydrogenase for the synthesis of L-glutamate. This work suggests that proflavine has the potential to become an efficient light-harvesting component in biocatalytic photosynthesis driven by solar energy.
authors.affiliation: KAIST Institute for the BioCentury, Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), 335 Science Road, Daejeon 305-701, South Korea.
authors.forename: Dong Heon
authors.initials: DH
authors.lastname: Nam
authors.forename: Chan Beum
authors.initials: CB
authors.lastname: Park
mesh_terms: D055162:Biocatalysis / Q000187:drug effects* / Q000528:radiation effects*; D018698:Glutamic Acid / Q000096:biosynthesis; D008027:Light; D009243:NAD / Q000378:metabolism*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D010788:Photosynthesis / Q000187:drug effects / Q000528:radiation effects; D011370:Proflavine / Q000494:pharmacology*
chemical_list: D017319:Photosensitizing Agents; D009243:NAD; D018698:Glutamic Acid; D011370:Proflavine