title: An AFLP-based approach for the identification of  sex-linked markers in blunt snout bream, Megalobrama amblycephala (Cyprinidae).
abstract: Sex-specific DNA markers are useful for studying  sex-determination mechanisms and establishment of monosex populations. Three  widely spaced geographical populations (Liangzi, Poyang and Yuni Lakes in  China) of blunt snout bream (Megalobrama amblycephala) were screened  with AFLPs to search for sex-linked markers. Female and male pools (10  individuals in each pool) from each population were screened using 64 different  primer combinations. A total of 4789 genomic fragments were produced, with a  mean frequency of 75 bands per primer pair. Three different primer combinations  produced putative sex-associated amplifications and were selected for  individual screening in the three populations. However, none showed sex  specificity when we converted these three markers into sequence characterized  amplified region markers and evaluated all the individuals from the three  populations.
authors.affiliation: College of Fishery, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: H O
authors.initials: HO
authors.lastname: Rao
authors.forename: J C
authors.initials: JC
authors.lastname: Deng
authors.forename: W M
authors.initials: WM
authors.lastname: Wang
authors.forename: Z-X
authors.initials: ZX
authors.lastname: Gao
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001483:Base Sequence; D003530:Cyprinidae / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D012737:Sex Factors
chemical_list: D017931:DNA Primers; D005819:Genetic Markers