title: Molecular typing of fecal eukaryotic microbiota of human infants and their respective mothers.
abstract: The micro-eukaryotic diversity from the human gut was investigated using universal primers directed towards 18S rRNA gene, fecal samples being the source of DNA. The subjects in this study included two breast-fed and two formula-milk-fed infants and their mothers. The study revealed that the infants did not seem to harbour any microeukaryotes in their gut. In contrast, there were distinct eukaryotic microbiota present in the mothers. The investigation is the first of its kind in the comparative study of the human feces to reveal the presence of micro-eukaryotic diversity variance in infants and adults from the Indian subcontinent. The micro-eukaryotes encountered during the investigation include known gut colonizers like Blastocystis and some fungi species. Some of these micro-eukaryotes have been speculated to  be involved in clinical manifestations of various diseases. The study is an attempt to highlight the importance of micro-eukaryotes  in the human gut.
authors.affiliation: Insect Molecular Biology Unit, National Centre for Cell Science, Pune University Campus, Ganeshkhind, Pune 411 007, Maharashtra, India.
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Pandey
authors.forename: Jay
authors.initials: J
authors.lastname: Siddharth
authors.forename: Pankaj
authors.initials: P
authors.lastname: Verma
authors.forename: Ashish
authors.initials: A
authors.lastname: Bavdekar
authors.forename: Milind S
authors.initials: MS
authors.lastname: Patole
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
mesh_terms: D000328:Adult; D044822:Biodiversity; D016844:Blastocystis / Q000235:genetics / Q000302:isolation & purification; D001942:Breast Feeding; D005243:Feces / Q000382:microbiology*; D005260:Female; D005658:Fungi / Q000235:genetics / Q000302:isolation & purification; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007223:Infant; D041943:Infant Formula; D054892:Metagenome; D058889:Molecular Typing / Q000379:methods; D009035:Mothers; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D012337:RNA, Ribosomal, 18S