title: Micronized acellular dermal matrix as an efficient expansion substrate and delivery vehicle of adipose-derived stem cells for vocal fold regeneration.
abstract: OBJECTIVE
  Cell therapy has been shown to prevent vocal fold scarring and atrophy. However, problems that include the expansion of large numbers of cells in vitro and the poor survival of transplanted cells in vivo must be solved. The aim of this study was to use micronized acellular dermal matrix (MADM) as an expansion substrate of rabbit allogeneic adipose-derived stem cells (ADSCs) and to apply the combination of the matrix and cells, ADSC-MADM, to vocal fold regeneration.
  
  
  METHODS
  Animal experiment.
  
  
  METHODS
  The proliferation of ADSCs that were cultured on the MADM was evaluated using 3-(4,5-dimethylthizazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfonyl)-2H-tetrazolium, and a rabbit acute vocal fold injury model was established by laser injury. Eighteen New Zealand white rabbits were randomly divided into three groups, which were injected with ADSC-MADM, ADSCs, and MADM, respectively. Morphological analysis was performed by laryngoscope, and histological analyses were indicated by hematoxylin and eosin staining, van Gieson staining, and immunofluorescence. Additionally, the in vivo survival of the ADSCs was determined by CM-Dil cell labeling.
  
  
  RESULTS
  When compared with a two-dimensional culture, the MADM significantly promoted proliferation of ADSCs. Morphological and histological analyses indicated that, when compared to only using of MAMD or the nontreatment sample, the use of ADSC-MADM or only using ADSCs successfully prevent scarring and atrophy. Moreover, ADSC-MADM exhibited a better therapeutic effect than when only using ADSCs, which was probably due to the MADM significantly enhancing the survival of transplanted ADSCs.
  
  
  CONCLUSIONS
  MADM could be used as an efficient expansion substrate and delivery vehicle for ADSCs in vocal fold regeneration.
authors.affiliation: Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liang
authors.forename: Shiyu
authors.initials: S
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Han
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Deng
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D017667:Adipocytes / Q000637:transplantation*; D000818:Animals; D001284:Atrophy; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002921:Cicatrix / Q000473:pathology / Q000517:prevention & control; D005109:Extracellular Matrix; D008562:Membrane Glycoproteins; D011817:Rabbits; D012038:Regeneration / Q000502:physiology*; D033581:Stem Cell Transplantation / Q000379:methods*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014827:Vocal Cords / Q000473:pathology / Q000601:surgery*
chemical_list: D008562:Membrane Glycoproteins; D013778:Tetrazolium Salts; D013844:Thiazoles; C048022:substrate adhesion molecules; C070380:3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium