title: Determination of gene expression patterns by whole-mount in situ hybridization.
abstract: Whole-mount in situ hybridization (WISH) is a reliable and specific method to study three-dimensional patterns of gene expression. A labeled nucleic acid probe anneals to a complementary target sequence and is visualized and localized in the embryo. This chapter describes a sensitive method for WISH on mouse embryos using digoxigenin-labeled RNA probes. The technique can be used for the analysis of gene expression patterns during early stages of odontogenesis and in tooth explants.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.
authors.forename: Sergiy
authors.initials: S
authors.lastname: Kyryachenko
authors.forename: Kateryna
authors.initials: K
authors.lastname: Kyrylkova
authors.forename: Mark
authors.initials: M
authors.lastname: Leid
authors.forename: Chrissa
authors.initials: C
authors.lastname: Kioussi
mesh_terms: D000818:Animals; D004076:Digoxigenin / Q000737:chemistry; D004622:Embryo, Mammalian / Q000378:metabolism*; D015870:Gene Expression / Q000235:genetics*; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D015347:RNA Probes / Q000737:chemistry
chemical_list: D015347:RNA Probes; D004076:Digoxigenin