title: Proteomic analysis of purified Newcastle disease virus particles.
abstract: BACKGROUND
  Newcastle disease virus (NDV) is an enveloped RNA virus, bearing severe economic losses to the poultry industry worldwide. Previous virion proteomic studies have shown that enveloped viruses carry multiple host cellular proteins both internally and externally during their life cycle. To address whether it also occurred during NDV infection, we performed a comprehensive proteomic analysis of highly purified NDV La Sota strain particles.
  
  
  RESULTS
  In addition to five viral structural proteins, we detected thirty cellular proteins associated with purified NDV La Sota particles. The identified cellular proteins comprised several functional categories, including cytoskeleton proteins, annexins, molecular chaperones, chromatin modifying proteins, enzymes-binding proteins, calcium-binding proteins and signal transduction-associated proteins. Among these, three host proteins have not been previously reported in virions of other virus families, including two signal transduction-associated proteins (syntenin and Ras small GTPase) and one tumor-associated protein (tumor protein D52). The presence of five selected cellular proteins (i.e., β-actin, tubulin, annexin A2, heat shock protein Hsp90 and ezrin) associated with the purified NDV particles was validated by Western blot or immunogold labeling assays.
  
  
  CONCLUSIONS
  The current study presented the first standard proteomic profile of NDV. The results demonstrated the incorporation of cellular proteins in NDV particles, which provides valuable information for elucidating viral infection and pathogenesis.
authors.affiliation: School of Environmental Science and Public Health, Wenzhou Medical College, Wenzhou, 325035, Peoples Republic of China.|State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, Peoples Republic of China.
authors.forename: Xiangpeng
authors.initials: X
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, Peoples Republic of China.
authors.forename: Chunyi
authors.initials: C
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, Peoples Republic of China.
authors.forename: Qingming
authors.initials: Q
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, Peoples Republic of China.
authors.forename: Chengwen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, 510642, Peoples Republic of China.
authors.forename: Yingzuo
authors.initials: Y
authors.lastname: Bi
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, Peoples Republic of China.
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Cao