title: A highly selective and sensitive fluorescence "turn-on" probe for Ag+ in aqueous solution and live cells.
abstract: A naphthalimide-based fluorescent probe, NPQ, that contains a novel receptor was successfully developed. NPQ exhibited "turn-on" fluorescence and excellent selectivity toward Ag(+) in the presence of various other metal ions in aqueous solution. A series of control compounds were designed and synthesized in order to explore the photoinduced electron transfer (PET) quenching mechanism of NPQ and binding mode of NPQ with Ag(+). Moreover, with the NPQ-Ag(+) complex, I(-) was easily selectively recognized by a marked fluorescence quenching. The live cell imaging experiments demonstrate that NPQ can be used as a fluorescent probe for monitoring Ag(+) in living cells.
authors.affiliation: Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Yufang
authors.initials: Y
authors.lastname: Xu
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhu
authors.forename: Chunmei
authors.initials: C
authors.lastname: Yang
authors.forename: Le
authors.initials: L
authors.lastname: Han
authors.forename: Xuhong
authors.initials: X
authors.lastname: Qian
mesh_terms: D002470:Cell Survival; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D015195:Drug Design; D004579:Electron Transport; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053644:Naphthalimides / Q000138:chemical synthesis / Q000737:chemistry*; D055668:Photochemical Processes; D012834:Silver / Q000032:analysis* / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D012996:Solutions; D014867:Water; D012834:Silver