title: Delayed reproductive dysfunction in female rats induced by early life exposure to low-dose diethylstilbestrol.
abstract: A one-lifespan test was carried out to establish a test protocol for endocrine-disrupting chemicals (EDCs). Diethylstilbestrol was administered by oral gavage to neonatal rats at doses of 0.05, 0.5 and 5 μg/kg/day for 5 days after birth. Abnormal estrous cycles were observed throughout the study in all females from the 5 μg/kg group, and in 40% from the 0.5 μg/kg group from 24 weeks of age. The conception rate of 12-week-old females in the 5 μg/kg group was 0%, and that of the 23-week-old females in the 0.5 μg/kg group was 33.3%. No effect of DES was observed at the first parturition in any group, except for the 5 μg/kg group. However, litter size was significantly reduced in the 0.5 μg/kg group at the second parturition. These results indicated that a prolonged period of observation of reproductive function is necessary to determine EDCs reliably.
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, 729-5 Ochiai, Hadano, Kanagawa 257-8523, Japan. ohta.r@fdsc.or.jp
authors.forename: Ryo
authors.initials: R
authors.lastname: Ohta
authors.forename: Hideo
authors.initials: H
authors.lastname: Ohmukai
authors.forename: Hideki
authors.initials: H
authors.lastname: Marumo
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shindo
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nagata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ono
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004912:Erythrocytes / Q000276:immunology; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D005298:Fertility / Q000187:drug effects; D008098:Litter Size / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects*; D012741:Sexual Maturation / Q000187:drug effects; D012756:Sheep; D013076:Sperm Count
chemical_list: D004968:Estrogens, Non-Steroidal; D004054:Diethylstilbestrol