title: Determination of eprosartan mesylate and hydrochlorothiazide in tablets by derivative spectrophotometric and high-performance liquid chromatographic methods.
abstract: Two new simple and selective assay methods have been presented for the analysis of eprosartan mesylate (EPR) and hydrochlorothiazide (HCT) in pharmaceutical formulations. The first method is based on first-derivative ultraviolet spectrophotometry with zero-crossing measurements at 246 and 279 nm for EPR and HCT, respectively. The assay was linear over the concentration ranges 3.0-14.0 µg/mL for EPR and 1.0-12.0 µg/mL for HCT. The quantification limits for EPR and HCT were found to be 1.148 and 0.581 µg/mL, respectively, while the detection limits were 0.344 µg/mL for EPR and 0.175 µg/mL for HCT. The second method involved isocratic reversed-phase liquid chromatography using a mobile phase composed of acetonitrile-10 mM phosphoric acid (pH 2.5) (40:60, v/v). Olmesartan was used as internal standard and the substances were detected at 272 nm. The linearity ranges were found to be 0.5-30 and 0.3-15.0 µg/mL for EPR and HCT, respectively. The limits of detection were found to be 0.121 µg/mL for EPR and 0.045 µg/mL for HCT. The limits of quantification were found to be 0.405 and 0.148 µg/mL for EPR and HCT, respectively. The proposed methods were successfully applied to the determination of commercially available tablets with a high percentage of recovery and good accuracy and precision.
authors.affiliation: Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, 34116, Beyazit, Istanbul, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Hacioğlu
authors.forename: Armağan
authors.initials: A
authors.lastname: Onal
mesh_terms: D000179:Acrylates / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D004355:Drug Stability; D006852:Hydrochlorothiazide / Q000032:analysis* / Q000737:chemistry; D007093:Imidazoles / Q000032:analysis* / Q000737:chemistry; D013607:Tablets / Q000032:analysis / Q000737:chemistry; D013876:Thiophenes / Q000032:analysis* / Q000737:chemistry
chemical_list: D000179:Acrylates; D007093:Imidazoles; D013607:Tablets; D013876:Thiophenes; D006852:Hydrochlorothiazide; C068373:eprosartan