title: Solution-based targeted genomic enrichment for precious DNA samples.
abstract: BACKGROUND
  Solution-based targeted genomic enrichment (TGE) protocols permit selective sequencing of genomic regions of interest on a massively parallel scale. These protocols could be improved by: 1) modifying or eliminating time consuming steps; 2) increasing yield to reduce input DNA and excessive PCR cycling; and 3) enhancing reproducible.
  
  
  RESULTS
  We developed a solution-based TGE method for downstream Illumina sequencing in a non-automated workflow, adding standard Illumina barcode indexes during the post-hybridization amplification to allow for sample pooling prior to sequencing. The method utilizes Agilent SureSelect baits, primers and hybridization reagents for the capture, off-the-shelf reagents for the library preparation steps, and adaptor oligonucleotides for Illumina paired-end sequencing purchased directly from an oligonucleotide manufacturing company.
  
  
  CONCLUSIONS
  This solution-based TGE method for Illumina sequencing is optimized for small- or medium-sized laboratories and addresses the weaknesses of standard protocols by reducing the amount of input DNA required, increasing capture yield, optimizing efficiency, and improving reproducibility.
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA.
authors.forename: Aiden Eliot
authors.initials: AE
authors.lastname: Shearer
authors.forename: Michael S
authors.initials: MS
authors.lastname: Hildebrand
authors.forename: Richard J H
authors.initials: RJ
authors.lastname: Smith
mesh_terms: D004247:DNA / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA