title: [Effect of EBV immediate-early protein Zta on the cell cycle of Daudi cells and its mechanisms].
abstract: OBJECTIVE
  To investigate the effect of EBV immediate-early protein Zta on cell cycle of Daudi cells and the involved mechanisms.
  
  
  METHODS
  The expression vector encoding Zta was constructed and electroporated into Daudi cells. Flow cytometric analysis was used to detect the cell cycle, Western blot to the protein levels of p21, Rb and E2F-1.
  
  
  RESULTS
  The vector was constructed successfully, the expression of Zta protein inhibited the proliferation of Daudi cells and promoted cell cycle from G(0)/G(1) phase \[(30.0 ± 3.4)%\] to S phase \[(47.7 ± 1.1)%\]. Meanwhile, Rb expression was significantly downregulated, E2F-1 and p21 expression upregulated by Zta.
  
  
  CONCLUSIONS
  Zta could promote G(0)/G(1) phase to S phase transition in Daudi cells, which might be associated with the reduced expression of Rb and increased expression of E2F-1 and p21 protein.
authors.affiliation: Qilu Children's Hospital of Shandong University, Jinan, China.
authors.forename: Qing-wei
authors.initials: QW
authors.lastname: Guo
authors.forename: Jin-dong
authors.initials: JD
authors.lastname: Guo
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Liu
authors.forename: Yun-ze
authors.initials: YZ
authors.lastname: Lang
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Zhang
authors.forename: Guo-sheng
authors.initials: GS
authors.lastname: Jiang
mesh_terms: D002453:Cell Cycle / Q000235:genetics*; D002455:Cell Division; D045744:Cell Line, Tumor; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D050687:E2F1 Transcription Factor / Q000378:metabolism; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D016160:Retinoblastoma Protein / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*; D015533:Transcriptional Activation; D014764:Viral Proteins / Q000235:genetics*
chemical_list: C063676:BZLF1 protein, Herpesvirus 4, Human; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D017874:Immediate-Early Proteins; D016160:Retinoblastoma Protein; D015534:Trans-Activators; D014764:Viral Proteins