title: A comparison of Val81Met and other polymorphisms of alcohol metabolising genes in patients and controls in Northern Spain.
abstract: The aim of this paper is to study polymorphism in the TH, ADH1B, ADH1C, ALDH2 and CYP2E1 genes so as to ascertain whether it is associated with excessive consumption of alcohol. The SNPs rs6356 of TH, rs1229984, rs2066702 of ADH1B; rs698, rs1693482 of ADH1C; rs671 of ALDH2; rs72559710, rs55897648, rs6413419, rs3813867, rs2031920, rs6413432 of CYP2E1 were studied in a sample of 172 high-level patients and 150 fully non-drinkers controls. Genotyping was performed using Rt-PCR with Taqman probes. SNPs located at ALDH2 and CYP2E1 showed no heterozygosity. Frequency distribution showed significant differences between the two groups studied for loci TH and ADH1B. The genotype Val/Val of TH locus increased in risk 1.988 times (95% CI: 1.006-3.930) that the subjects carrying the genotype Met/Met; and the genotype ADH1B*1/*1 of ADH1B locus increased in risk 3.811 times (CI: 1.660-8.749) that the subjects carrying the genotype ADH1B*1/*2. Alleles Val and ADH1B*1 may therefore increase the risk of the onset and development of this illness.
authors.affiliation: BIOMICs Research Group, CIBERehd, University of Basque Country UPV/EHU, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Celorrio
authors.forename: Luis
authors.initials: L
authors.lastname: Bujanda
authors.forename: Carlos
authors.initials: C
authors.lastname: Caso
authors.forename: Miguel
authors.initials: M
authors.lastname: Landabaso
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Oria
authors.forename: Javier
authors.initials: J
authors.lastname: Ogando
authors.forename: Marian M
authors.initials: MM
authors.lastname: de Pancorbo
mesh_terms: D000328:Adult; D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000437:Alcoholism / Q000453:epidemiology / Q000235:genetics*; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D013030:Spain / Q000453:epidemiology; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics; D044465:White People / Q000235:genetics
chemical_list: C489332:ADH1B protein, human; D000426:Alcohol Dehydrogenase; D019392:Cytochrome P-450 CYP2E1; D014446:Tyrosine 3-Monooxygenase