title: Oxidative stress and antioxidant defense in patients with chronic hepatitis B.
abstract: BACKGROUND
  Oxidative stress is defined as a disturbance of balance between free radicals and antioxidant defense system. This study investigated oxidative stress in patients with chronic hepatitis B.
  
  
  METHODS
  Sixty nine patients with chronic hepatitis B admitted to the Department of the Infectious Diseases and Clinical Microbiology of Medical Faculty of Ondokuz Mayis University were enrolled into study. Twenty healthy persons were included as a control group. The study group was divided into three groups: healthy controls (group 1), chronic hepatitis B (group 2), and inactive hepatitis B carriers (group 3). Antioxidant status of plasma, including glutathione, glutathione peroxidase, vitamin E, and vitamin C levels were measured. Carbonyl and lipid peroxidation levels were measured as parameters of oxidative stress.
  
  
  RESULTS
  Glutathione, glutathione peroxidase, vitamin E, and vitamin C levels were found to be significantly decreased in the chronic hepatitis B group when compared with the control group (9.5 vs. 13.8, p < 0.05; 22.98 vs. 32.4, p < 0.05; 15.1 vs. 16.4, p < 0.05; 12.9 vs. 18.4, p < 0.05, respectively). Carbonyl and lipid peroxidation levels were significantly increased in the chronic hepatitis B group compared to controls (0.7 vs. 0.5, p < 0.05; 2 vs. 0.7, p < 0.05, respectively). However, whereas the glutathione and carbonyl level correlation with HBV DNA levels were mild to moderate (glutathione vs. HBV DNA, r:-0.288, p < 0.05; carbonyl vs. HBV DNA, r:0.317, p < 0.05), the lipid peroxidation levels were strongly related with HBV DNA levels in chronic hepatitis B (r:0.545, p < 0.05).
  
  
  CONCLUSIONS
  Oxidative stress was significantly increased in hepatitis B patients. Consequently, decreases were seen at the level of protective antioxidative parameters in the blood of these patients.
authors.affiliation: Ondokuz Mayis University, Faculty of Medicine, Department of Clinical Microbiology and Infectious Diseases, Samsun, Turkey. nuriyetf@omu.edu.tr
authors.forename: Nuriye
authors.initials: N
authors.lastname: Tasdelen Fisgin
authors.forename: Birsen Kilicoglu
authors.initials: BK
authors.lastname: Aydin
authors.forename: Hanife
authors.initials: H
authors.lastname: Sarikaya
authors.forename: Esra
authors.initials: E
authors.lastname: Tanyel
authors.forename: Saban
authors.initials: S
authors.lastname: Esen
authors.forename: Mustafa
authors.initials: M
authors.lastname: Sunbul
authors.forename: Hakan
authors.initials: H
authors.lastname: Leblebicioglu
mesh_terms: D000328:Adult; D001205:Ascorbic Acid / Q000097:blood; D002353:Carrier State / Q000378:metabolism* / Q000821:virology; D016022:Case-Control Studies; D005260:Female; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D019694:Hepatitis B, Chronic / Q000378:metabolism* / Q000821:virology; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D050050:Protein Carbonylation; D019562:Viral Load; D014810:Vitamin E / Q000097:blood
chemical_list: D014810:Vitamin E; D005979:Glutathione Peroxidase; D005978:Glutathione; D001205:Ascorbic Acid