title: Alginate and alginate/gelatin microspheres for human adipose-derived stem cell encapsulation and differentiation.
abstract: Human adipose-derived stem cells (hADSC) encapsulated in alginate and alginate/gelatin microspheres with adjustable properties were fabricated via an improved microsphere generating device. The mechanism of the device, porous property, swelling behavior of the microspheres and hADSC proliferation as well as adipogenic differentiation were studied extensively. Microspheres with high-ratio evenly distributed adipocytes could be obtained by utilizing the proper matrix material and manufacturing parameters. The adipocyte/hADSC microspheres were a sound in vitro mimicking of a natural fat lobule and therefore a good candidate for adipose tissue engineering and regenerative medicine.
authors.affiliation: Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. yaorui@tsinghua.edu.cn
authors.forename: Rui
authors.initials: R
authors.lastname: Yao
authors.forename: Renji
authors.initials: R
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Luan
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000273:Adipose Tissue / Q000166:cytology*; D000464:Alginates / Q000737:chemistry* / Q000494:pharmacology; D002214:Capsules; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008863:Microspheres; D010316:Particle Size; D016062:Porosity; D013234:Stem Cells / Q000166:cytology*
chemical_list: D000464:Alginates; D002214:Capsules; D004337:Drug Carriers; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005780:Gelatin