title: Expression and analysis of a cytolethal distending toxin (cdt) gene operon in Campylobacter lari.
abstract: The present study examines the expression of cytolethal distending toxin (cdt) gene encoding a cytotoxin in Campylobacter lari (n=6 urease-negative [UN] C. lari; n=4 urease-positive thermophilic Campylobacter [UPTC]). When reverse transcription polymerase chain reaction (RT-PCR) was carried out with 10 C. lari isolates using a primer pair to amplify the cdtB gene transcript segment, an approximate 260 bp RT-PCR amplicon was generated with all the isolates. In addition, cdtA, cdtB and cdtC gene operon was identified to be polycistronicly transcribed in the C. lari cells. The cdtB gene translation in the C. lari cells was also confirmed by Western blot analysis. Thus, the cdt gene operon in C. lari organisms, including UN C. lari and UPTC, was expressed at the transcriptional and translational levels in the cells. The present results suggest that all three cdt genes may be functional in the cells.
authors.affiliation: Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Sagamihara, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: A
authors.initials: A
authors.lastname: Tazumi
authors.forename: J
authors.initials: J
authors.lastname: Hirayama
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: E
authors.initials: E
authors.lastname: Tasaki
authors.forename: M
authors.initials: M
authors.lastname: Asakura
authors.forename: S
authors.initials: S
authors.lastname: Yamasaki
authors.forename: J E
authors.initials: JE
authors.lastname: Moored
authors.forename: B C
authors.initials: BC
authors.lastname: Millar
authors.forename: K
authors.initials: K
authors.lastname: Matsubarak
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002169:Campylobacter Infections / Q000235:genetics / Q000382:microbiology; D044863:Campylobacter lari / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009876:Operon; D014176:Protein Biosynthesis; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001427:Bacterial Toxins; C067044:cytolethal distending toxin