title: Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast.
abstract: The mechanism of cargo sorting at the trans-Golgi network (TGN) for secretion is poorly understood. We previously reported the involvement of the actin-severing protein cofilin and the Ca(2+) ATPase secretory pathway calcium ATPase 1 (SPCA1) in the sorting of soluble secretory cargo at the TGN in mammalian cells. Now we report that cofilin in yeast is required for export of selective secretory cargo at the late Golgi membranes. In cofilin mutant (cof1-8) cells, the cell wall protein Bgl2 was secreted at a reduced rate and retained in a late Golgi compartment, whereas the plasma membrane H(+) ATPase Pma1, which is transported in the same class of carriers, reached the cell surface. In addition, sorting of carboxypeptidase Y (CPY) to the vacuole was delayed, and CPY was secreted from cof1-8 cells. Loss of the yeast orthologue of SPCA1 (Pmr1) exhibited similar sorting defects and displayed synthetic sickness with cof1-8. In addition, overexpression of PMR1 restored Bgl2 secretion in cof1-8 cells. These findings highlight the conserved role of cofilin and SPCA1/Pmr1 in sorting of the soluble secretory proteins at the TGN/late Golgi membranes in eukaryotes.
authors.affiliation: Department of Cell and Developmental Biology, Centre for Genomic Regulation, 08003 Barcelona, Spain.
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Curwin
authors.forename: Julia
authors.initials: J
authors.lastname: von Blume
authors.forename: Vivek
authors.initials: V
authors.lastname: Malhotra
mesh_terms: D051339:Actin Depolymerizing Factors / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D043402:Cathepsin A / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D051338:Cofilin 1 / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009154:Mutation; D021381:Protein Transport; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism; D050683:R-SNARE Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism*
chemical_list: D051339:Actin Depolymerizing Factors; C495813:COF1 protein, S cerevisiae; D051338:Cofilin 1; D050683:R-SNARE Proteins; C083241:SNC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; C080389:BGL2 protein, S cerevisiae; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D043402:Cathepsin A; C092477:PMA1 protein, S cerevisiae; D006180:Proton-Translocating ATPases