title: Antimicrobial resistance patterns and their encoding genes among Acinetobacter baumannii strains isolated from burned patients.
abstract: The purpose of this study was to determine the mechanisms and patterns of antimicrobial resistance among the isolates obtained from burned patients with wound infections at a teaching hospital in Tehran, Iran. A total of 23 Acinetobacter baumannii isolates were collected from patients with burn wound infections between August 2009 and July 2010 from a hospital in Tehran. The susceptibility of these strains against 11 antimicrobial agents was determined by E-test according to the CLSI guidelines. All the resistant strains were then subjected to PCR assay for 28 distinct resistance genes. The most active antimicrobial agent was colistin with 100% sensitivity followed by gentamicin, amikacin and imipenem with 69.5%, 52.1% and 51.1% sensitivity, respectively. The most frequent resistance genes detected were bla(OXA-51-like) genes (n=23; 100%) that was intrinsic to A. baumannii isolates, gyrA (n=23; 100%), carO (n=23; 100%), tetA (n=22; 95.5%), tetB (n=15; 65.2%), intI (n=13; 56.5%) and PER (n=12; 52.1%), respectively. In order to make a proper choice of antibiotic for burn patients, it would be beneficial to physicians to identify drug resistance patterns in A. baumannii isolates.
authors.affiliation: Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Parisa
authors.initials: P
authors.lastname: Asadollahi
authors.forename: Mahdi
authors.initials: M
authors.lastname: Akbari
authors.forename: Setareh
authors.initials: S
authors.lastname: Soroush
authors.forename: Morovat
authors.initials: M
authors.lastname: Taherikalani
authors.forename: Khairollah
authors.initials: K
authors.lastname: Asadollahi
authors.forename: Kourosh
authors.initials: K
authors.lastname: Sayehmiri
authors.forename: Abbas
authors.initials: A
authors.lastname: Maleki
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Maleki
authors.forename: Parviz
authors.initials: P
authors.lastname: Karimi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Emaneini
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002056:Burns / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D007492:Iran; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents