title: Changes in the level of serum high-temperature requirement A1 (HtrA1) during pregnancy and its relationship to preeclampsia.
abstract: OBJECTIVE
  To investigate the expression and changes of high-temperature requirement A1 (HtrA1) during pregnancy and the use of this value in predicting preeclampsia.
  
  
  METHODS
  Serum samples were collected from pregnant mothers at different gestational weeks, and double-antibody sandwich enzyme-linked immunosorbent assay was employed to describe the changes in HtrA1 in serum during pregnancy.
  
  
  RESULTS
  (i) In Xi'an area of China, the incidence of preeclampsia was 4.95%, including 0.85% of early-onset type and 4.10% of late-onset type; (ii) the HtrA1 showed a lognormal distribution during pregnancy in the maternal serum, with the peak at 17-20 weeks of pregnancy; (iii) the HtrA1 levels in preeclampsia mothers peaked at 13-16 weeks of pregnancy, followed by acute decline until 21-24 weeks, then remained stable; (iv) the HtrA1 levels in preeclampsia mothers were higher than the control group in 13-16 weeks and lower than that in 21-24 weeks (p < 0.05); (v) the criteria using lgHtrA1 level at 1.684 during 13-16 weeks of pregnancy could predict hypertension disorders complicating pregnancy (HDCP) with sensitivity of 62.1% and specificity of 53.7%. This could be improved to 85 and 83%, respectively, when combined with body mass index as well as education background of the mother.
  
  
  CONCLUSIONS
  (i) HtrA1 showed lognormal distribution during pregnancy for all populations; (ii) HtrA1 level peaked at 17-20 weeks in normal group of pregnant mothers, and the increase of HtrA1 level in 13-16 weeks could predict the risk of preeclampsia; (iii) the risk calculation formula for preeclampsia: p (%) = eY/(1 + eY) (Y = -15.87 + 3.706 × lgHtrA1 + 0.134 × mean arterial pressure (MAP) - 1.4 × education level code); e = 2.718.
authors.affiliation: Department of Obstetrics & Gynecology, First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zong
authors.forename: Wenli
authors.initials: W
authors.lastname: Gou
authors.forename: Wenyu
authors.initials: W
authors.lastname: Shao
authors.forename: Pu
authors.initials: P
authors.lastname: Huang
authors.forename: Chunfang
authors.initials: C
authors.lastname: Li
mesh_terms: D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D000074289:High-Temperature Requirement A Serine Peptidase 1; D006801:Humans; D007231:Infant, Newborn; D011225:Pre-Eclampsia / Q000097:blood* / Q000453:epidemiology; D011247:Pregnancy; D012016:Reference Values; D012697:Serine Endopeptidases / Q000097:blood*
chemical_list: D015415:Biomarkers; D000074289:High-Temperature Requirement A Serine Peptidase 1; C474012:HTRA1 protein, human; D012697:Serine Endopeptidases