title: Development of microsatellite primers for Jatropha curcas (Euphorbiaceae) and transferability to congeners.
abstract: OBJECTIVE
  Microsatellite primers were developed for Jatropha curcas (Euphorbiaceae), a tree species with large potential for biofuel production, to investigate its natural genetic diversity and mating system to facilitate the establishment of tree improvement and conservation programs.
  
  
  RESULTS
  Using a protocol for genomic library enrichment, 104 clones containing 195 repeat motifs were identified. Primer pairs were developed for 40 microsatellite loci and validated in 41 accessions of J. curcas from six provenances. Nine loci were polymorphic revealing from two to eight alleles per locus, and six primers were able to amplify alleles in the congeners J. podagrica, J. pohliana, and J. gossypifolia, but not in other Euphorbiaceae species, such as Hevea brasiliensis, Manihot esculenta, or Ricinus communis.
  
  
  CONCLUSIONS
  The primers developed here revealed polymorphic loci that are suitable for genetic diversity and structure, mating system, and gene flow studies in J. curcas, and some congeners.
authors.affiliation: Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Av. Centenário, 303, CP 96, Piracicaba, São Paulo 13400-970, Brazil.
authors.forename: Eduardo de A
authors.initials: Ede A
authors.lastname: Bressan
authors.forename: Danielle C
authors.initials: DC
authors.lastname: Scotton
authors.forename: Renato R
authors.initials: RR
authors.lastname: Ferreira
authors.forename: Erika C
authors.initials: EC
authors.lastname: Jorge
authors.forename: Alexandre M
authors.initials: AM
authors.lastname: Sebbenn
authors.forename: Lee T S
authors.initials: LT
authors.lastname: Gerald
authors.forename: Antonio
authors.initials: A
authors.lastname: Figueira
mesh_terms: D000483:Alleles; D002367:Ricinus communis / Q000235:genetics; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D028482:Hevea / Q000235:genetics; D031286:Jatropha / Q000145:classification / Q000235:genetics*; D002365:Manihot / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant