title: Chlorogenic acid inhibits LPS-induced microglial activation and improves survival of dopaminergic neurons.
abstract: Pro-inflammatory factors released by activated microglia may contribute to the progression of neurodegenerative diseases. As a natural phenolic acid, chlorogenic acid (CGA) has been shown to have anti-inflammatory properties. However, it is unclear whether CGA has the ability to mediate microglial activation. The present study investigated the role of CGA in lipopolysaccharide (LPS)-stimulated microglia. Our data demonstrated that CGA significantly suppressed NO production and TNF-α release in LPS-stimulated primary microglia. In addition, CGA decreased LPS-stimulated phosphorylation and degradation of inhibitory kappa B-alpha (IκBα), and prevented translocation of nuclear factor-kappaB (NF-κB). Furthermore, CGA prevented neurotoxicity caused by microglial activation and ultimately improved survival of dopaminergic (DA) neuron. Finally, in vivo data showed that CGA pretreatment attenuated LPS-induced IL-1β and TNF-α release in substantia nigra (SN). Our results suggested that the pretreatment of CGA significantly inhibits the microglial activation, and CGA may be neuroprotective for pro-inflammatory factor-mediated neurodegenerative disorders.
authors.affiliation: Department of Pathophysiology, School of Medicine, Jinan University, Guangzhou, Guangdong 510632, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Shen
authors.forename: Renbin
authors.initials: R
authors.lastname: Qi
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.forename: Huadong
authors.initials: H
authors.lastname: Wang
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Hu
authors.forename: Yanru
authors.initials: Y
authors.lastname: Zhao
authors.forename: Man
authors.initials: M
authors.lastname: Bie
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Fu
authors.forename: Mengfei
authors.initials: M
authors.lastname: Chen
authors.forename: Daxiang
authors.initials: D
authors.lastname: Lu
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000494:pharmacology*; D059290:Dopaminergic Neurons / Q000187:drug effects* / Q000502:physiology*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000187:drug effects* / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides; D002726:Chlorogenic Acid