title: 3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture.
abstract: In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Wan-Geun
authors.initials: WG
authors.lastname: La
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Bhang
authors.forename: Jung-Youn
authors.initials: JY
authors.lastname: Shin
authors.forename: Hee Hun
authors.initials: HH
authors.lastname: Yoon
authors.forename: Jooyeon
authors.initials: J
authors.lastname: Park
authors.forename: Hee Seok
authors.initials: HS
authors.lastname: Yang
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Yu
authors.forename: Yung-Eun
authors.initials: YE
authors.lastname: Sung
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D017209:Apoptosis; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000295:instrumentation*; D049109:Cell Proliferation; D002470:Cell Survival; D016895:Culture Media, Serum-Free / Q000378:metabolism*; D004295:Dihydroxyphenylalanine / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006801:Humans
chemical_list: D015815:Cell Adhesion Molecules; D016895:Culture Media, Serum-Free; D004295:Dihydroxyphenylalanine