title: Promotion of diabetic wound healing by collagen scaffold with collagen-binding vascular endothelial growth factor in a diabetic rat model.
abstract: OBJECTIVE
  Studies show that VEGF can promote tissue regeneration in diabetic wounds. The aim of this study was to evaluate the effects of a new composite biomaterial, a collagen scaffold with CBD-VEGF, for wound healing in a diabetic rat model.
  
  
  METHODS
  We produced a collagen scaffold loaded with CBD-VEGF, which allowed VEGF to bind to the collagen scaffold. The diabetic rat model was constructed by injecting streptozocin (STZ) peritoneally and removing a 2 x 2.5 cm thick slice of skin from the back of the animal. Animals were randomly divided into 4 groups: blank control (BC Group, n = 24), collagen scaffold loaded with PBS (PBS Group, n = 24), collagen scaffold loaded with NAT-VEGF (NAT-VEGF Group, n = 24), and collagen scaffold loaded with CBD-VEGF (CBD-VEGF Group, n = 24). Wounds of the BC Group were covered with gauze and those of the PBS, NAT-VEGF and CBD-VEGF Groups were grafted by corresponding collagen scaffolds, respectively. Healing rates were calculated and compared among groups. Wound tissue was evaluated by histologic analysis.
  
  
  RESULTS
  The CBD-VEGF group showed a higher wound healing rate, better vascularization and higher level of VEGF in the granulation tissue wound compared with NAT-VEGF and PBS groups.
  
  
  CONCLUSIONS
  The collagen scaffold with CBD-VEGF promoted wound healing in a diabetic rat model, which could potentially provide better therapeutic options for the treatment of diabetic wounds.
authors.affiliation: Department of burns and plastic surgery, Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Tan
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.forename: Yue
authors.initials: Y
authors.lastname: Lin
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Xianglin
authors.initials: X
authors.lastname: Hou
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001786:Blood Glucose / Q000032:analysis / Q000737:chemistry; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D048909:Diabetes Complications / Q000628:therapy*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004195:Disease Models, Animal; D006639:Histidine / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000473:pathology; D016038:Skin Transplantation; D013311:Streptozocin; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D001786:Blood Glucose; D042461:Vascular Endothelial Growth Factor A; C033223:polyhistidine; D006639:Histidine; D013311:Streptozocin; D003094:Collagen