title: [Study on protective effect of salvianolic acid B on glutamate-induced excitotoxicity in pheochromocytoma PC12 cells].
abstract: OBJECTIVE
  To study the protective effect and mechanism of salvianolic acid B (Sal B) on glutamate-induced excito-toxicity.
  
  
  METHODS
  Glutamate-induced PC12 cell injury model was established to detect the cell survival rate by MTT, the leakage rate of lactic dehydrogenases using LDH, and the cell apoptosis by using AO/EB double staining for fluorescence microscope and PI single staining flow cytometry which was also used to detect the content of intracellular reactive oxygen species. The expression of Caspase-3 protein was also detected by the Western blotting method.
  
  
  RESULTS
  Sal B is proved to inhibit glutamate-induced PC12 cells from injury and prevent them from releasing LDH within the range from 50 micromol x L(-1) to 200 micromol x L(-1). Meanwhile, Sal B has an effect on significantly reducing the expression of inhibit glutamate-induced active Caspase-3 protein, inhibiting accumulated glutamate-induced ROS and decreasing PC12 cell apoptosis rate within the range from 50 micromol x L(-1) to 200 micromol x L(-1).
  
  
  CONCLUSIONS
  The study proves that Sal B prevented against glutamate-induced cell injury via inhibiting ROS formation and Caspase-3 pathway-dependent apoptosis in PC12 cells.
authors.affiliation: Key Laboratory of Xin'an Medicine, Ministry of Education, Hefei 230038, China. wangxuncui@163.com
authors.forename: Xuncui
authors.initials: X
authors.lastname: Wang
authors.forename: Guoqi
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001572:Benzofurans / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D018698:Glutamic Acid / Q000009:adverse effects*; D045022:Lactate Dehydrogenases / Q000378:metabolism; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000378:metabolism*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D053630:Depsides
chemical_list: D001572:Benzofurans; D053148:Caspase 3; D004365:Drugs, Chinese Herbal; D018691:Excitatory Amino Acid Antagonists; D018698:Glutamic Acid; D045022:Lactate Dehydrogenases; D017382:Reactive Oxygen Species; C076944:salvianolic acid B; D053630:Depsides