title: Strategy for catch and release of azide-tagged biomolecules utilizing a photolabile strained alkyne construct.
abstract: A photolabile o-nitrobenzyl linker-cyclooctyne conjugate was prepared, immobilized on poly(methacrylate) beads and utilized as a trap for azide-functionalized compounds. These could be released by 365 nm UV light irradiation in high yield and purity. The "reagent-free" and time economic catch and release protocol was deemed useful for chemical proteomics applications.
authors.affiliation: Institute of Organic Chemistry, University of Tübingen, Auf der Morgenstelle 18, 72076 Tübingen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Golkowski
authors.forename: Carlo
authors.initials: C
authors.lastname: Pergola
authors.forename: Oliver
authors.initials: O
authors.lastname: Werz
authors.forename: Thomas
authors.initials: T
authors.lastname: Ziegler
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D015394:Molecular Structure; D055668:Photochemical Processes; D014466:Ultraviolet Rays
chemical_list: D000480:Alkynes; D001386:Azides