title: Sirt1 exerts anti-inflammatory effects and promotes steroidogenesis in Leydig cells.
abstract: OBJECTIVE
  To investigate the anti-inflammatory effects of Sirt1 and its roles in steroidogenesis in Leydig cells.
  
  
  METHODS
  In vitro cell culture study.
  
  
  METHODS
  Reproductive medical center.
  
  
  METHODS
  C57BL/6 male mice.
  
  
  METHODS
  TM3 Leydig cells were treated with proinflammatory cytokines including tumor necrosis factor-α, interleukin-6, or interleukin-1β.
  
  
  METHODS
  Sirt1 mRNA and protein levels were measured by real-time polymerase chain reaction and Western blotting in TM3 Leydig cells treated with proinflammatory cytokines. The cell viability was determined using MTT and BrdU incorporation assays. The effect of Sirt1 on the coactivation of steroidogenic factor 1 (SF-1) was characterized by coimmunoprecipitation and luciferase reporter assays.
  
  
  RESULTS
  Sirt1 mRNA and protein levels were significantly down-regulated by proinflammatory cytokines treatment in TM3 Leydig cells. Sirt1 agonist or overexpression could efficiently protect cytotoxicity induced by proinflammatory cytokines. Sirt1 promoted steroidogenesis through its coactivation of SF-1.
  
  
  CONCLUSIONS
  Sirt1 plays protective roles and promotes steroidogenesis in Leydig cells through its anti-inflammatory actions and coactivation of SF-1.
authors.affiliation: Reproductive Medical Center of Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wu
authors.forename: Aijun
authors.initials: A
authors.lastname: Zhang
authors.forename: Yijuan
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Zhu
authors.forename: Weimin
authors.initials: W
authors.lastname: Fan
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Lu
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000378:metabolism; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000494:pharmacology; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D034741:RNA, Small Interfering / Q000494:pharmacology; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D054339:Steroidogenic Factor 1 / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis*; D014162:Transfection; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics / Q000502:physiology
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D034741:RNA, Small Interfering; D054339:Steroidogenic Factor 1; D013256:Steroids; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1