title: Sequencing by hybridization of long targets.
abstract: Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.
authors.affiliation: School of Engineering and Applied Sciences and Kavli Institute for Bionano Science and Technology, Harvard University, Cambridge, Massachusetts, United States of America.
authors.forename: Yu
authors.initials: Y
authors.lastname: Qin
authors.forename: Tobias M
authors.initials: TM
authors.lastname: Schneider
authors.forename: Michael P
authors.initials: MP
authors.lastname: Brenner
mesh_terms: D004247:DNA / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D015342:DNA Probes; D004247:DNA