title: Analysis of expressed sequence tags from the antarctic psychrophilic green algae, Pyramimonas gelidicola.
abstract: Expressed sequence tags (ESTs) from the Antarctic green algae Pyramimonas gelidicola were analyzed to obtain molecular information on cold acclimation of psychrophilic microorganisms. A total of 2,112 EST clones were sequenced, generating 222 contigs and 219 singletons, and 200 contigs and 391 singletons from control (4 degrees C) and cold-shock conditions (-2 degrees C), respectively. The complete EST sequences were deposited to the DDBJ EST database (http:// www.ddbj.nig.ac.jp/index-e.html) and the nucleotide sequences reported in this study are available in the DDBJ/EMBL/ GenBank. These EST databases of Antarctic green algae can be used in a wide range of studies on psychrophilic genes expressed by polar microorganisms.
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 406-840, Korea.
authors.forename: Woongsic
authors.initials: W
authors.lastname: Jung
authors.forename: Sung Gu
authors.initials: SG
authors.lastname: Lee
authors.forename: Se Won
authors.initials: SW
authors.lastname: Kang
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Lee
authors.forename: Jun Hyuck
authors.initials: JH
authors.lastname: Lee
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Kang
authors.forename: Eon Seon
authors.initials: ES
authors.lastname: Jin
authors.forename: Hak Jun
authors.initials: HJ
authors.lastname: Kim
mesh_terms: D000864:Antarctic Regions; D000460:Chlorophyta / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects*; D003080:Cold Temperature; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological