title: Colonizing ability of Pseudomonas fluorescens 2112, among collections of 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens spp. in pea rhizosphere.
abstract: Pseudomonas fluorescens 2112, isolated in Korea as an indigenous antagonistic bacteria, can produce 2,4- diacetylphloroglucinol (2,4-DAPG) and the siderophore pyoveridin2112 for the control of phytophthora blight of red-pepper. P. fluorescens 2112 was classified into a new genotype C among the 17 genotypes of 2,4-DAPG producers, by phlD restriction fragment length polymorphism (RFLP). The colonizing ability of P. fluorescens 2112 in pea rhizosphere was equal to the well-known pea colonizers, P. fluorescens Q8r1 (genotype D) and MVP1-4 (genotype P), after 6 cycling cultivations for 18 weeks. Four tested 2,4- DAPG-producing Pseudomonas spp. could colonize with about a 96% dominance ratio against total bacteria in pea rhizosphere. The strain P. fluorescens 2112 was as good a colonizer as other Pseudomonas spp. genotypes in pea plant growth-promoting rhizobacteria.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, South Korea. sdkim@ynu.ac.kr
authors.forename: Sang-Dal
authors.initials: SD
authors.lastname: Kim
authors.forename: Leonardo De La
authors.initials: Lde L
authors.lastname: Fuente
authors.forename: David M
authors.initials: DM
authors.lastname: Weller
authors.forename: Linda S
authors.initials: LS
authors.lastname: Thomashow
mesh_terms: D000898:Antibiosis; D058448:Biota; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D007723:Korea; D058889:Molecular Typing; D018532:Pisum sativum / Q000382:microbiology*; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D011551:Pseudomonas fluorescens / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D058441:Rhizosphere; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; C059817:2,4-diacetylphloroglucinol; D010696:Phloroglucinol