title: Methanol extract of Desmodium gangeticum DC root mimetic post-conditioning effect in isolated perfused rat heart by stimulating muscarinic receptors.
abstract: OBJECTIVE
  To evaluate pharmacological mimetic action of herbal extract Desmodium gangeticum (DG) roots on ischemia reperfusion injury.
  
  
  METHODS
  With the help of Langendroff perfusion technique, ischemic post condition (POC) mimetic action of DG methanol root extract was evaluated and compared by using standard drugs that acts as muscarinic receptor agonist and antagonist, namely acetylcholine (Ach) and atropine (Atr) respectively in an isolated rat heart.
  
  
  RESULTS
  The physiological parameters like left ventricular developed pressure, end diastolic pressure and working index of isolated rat heart showed significant recovery in DG root extract administrated rat heart, similar to the recovery by POC. Kymogram results showed muscarinic receptor agonist like action for DG methanol root extract, confirmed in rat heart by muscarnic receptor agonist (acetylcholine) and anatoginst (atropine). Administration of DG root extract prior to reperfusion showed better antioxidant status in myocardial tissue homogenate and mitochondrial, complemented by the levels of cardiac specific marker proteins in myocardial tissue and perfusate. Even though DG methanol root extract mimics its action similar to that of Ach, the myocardial protection mediated by the extract was superior to Ach, due to the presence of antioxidants in the crude extract.
  
  
  CONCLUSIONS
  DG methanol root extract provides myocardial protection towards IRI by stimulating muscarinic receptors.
authors.affiliation: Medical Biochemistry, School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India. ginokurian@hotmail.com
authors.forename: Gino A
authors.initials: GA
authors.lastname: Kurian
authors.forename: Jose
authors.initials: J
authors.lastname: Paddikkala
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D007887:Fabaceae; D066298:In Vitro Techniques; D057775:Ischemic Postconditioning / Q000379:methods*; D008297:Male; D000432:Methanol / Q000494:pharmacology; D008929:Mitochondria, Heart / Q000378:metabolism; D018721:Muscarinic Agonists / Q000494:pharmacology; D018727:Muscarinic Antagonists / Q000494:pharmacology; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D015221:Potassium Channels / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000187:drug effects*
chemical_list: D002316:Cardiotonic Agents; C521776:Desmodium gangeticum extract; D018721:Muscarinic Agonists; D018727:Muscarinic Antagonists; D010936:Plant Extracts; D015221:Potassium Channels; D011976:Receptors, Muscarinic; C117780:mitochondrial K(ATP) channel; D000432:Methanol