title: [Cyclic digene system as a control element of a bacterial biosensor].
abstract: The Escherichia coil JC1 58(pCIA12/pG FK5) strain carrying a cyclic digene system with a negative feedback on the pCIA12 plasmid reacting to the DNA damage by changing the synthesis level of reporter genes-GFP and beta-galactosidase-was tested. The acquired phenotype was inherited by the next generations after the removal of the genotoxic action when the concentration of the DNA-damaging compounds was above the threshold level. A potential has been shown for the application of bacterial biosensors to monitor the presence of genotoxicants in the environment and to test the consequences of short-term exposures to toxic compounds.
authors.forename: E É
authors.initials: EÉ
authors.lastname: Stupak
authors.forename: I V
authors.initials: IV
authors.lastname: Stupak
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D015169:Colony Count, Microbial; D004249:DNA Damage / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D006861:Hydrogen Peroxide / Q000032:analysis / Q000494:pharmacology; D016685:Mitomycin / Q000032:analysis / Q000494:pharmacology; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis* / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013050:Spectrometry, Fluorescence; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D009153:Mutagens; D049452:Green Fluorescent Proteins; D016685:Mitomycin; D006861:Hydrogen Peroxide; D001616:beta-Galactosidase