title: 3,4,5-Trihydroxycinnamic Acid Inhibits LPS-Induced iNOS Expression by Suppressing NF-κB Activation in BV2 Microglial Cells.
abstract: Although various derivatives of caffeic acid have been reported to possess a wide variety of biological activities such as neuronal protection against excitotoxicity and anti-inflammatory property, the biological activity of 3,4,5-trihydroxycinnamic acid (THC), a derivative of hydroxycinnamic acids, has not been clearly examined. The objective of the present study is to evaluate the anti-inflammatory effects of THC on lipopolysaccharide (LPS)-stimulated BV2 microglial cells. THC significantly suppressed LPS-induced excessive production of nitric oxide (NO) and expression of iNOS, which is responsible for the production of iNOS. THC also suppressed LPS-induced overproduction of pro-inflammatory cytokines such as IL-1β and TNF-α in BV2 microgilal cells. Furthermore, THC significantly suppressed LPS-induced degradation of IκB, which retains NF-κB in the cytoplasm. Therefore, THC attenuated nuclear translocation of NF-κB, a major pro-inflammatory transcription factor. Taken together, the present study for the first time demonstrates that THC exhibits anti-inflammatory activity through the suppression of NF-κB transcriptional activation in LPS-stimulated BV2 microglial cells.
authors.affiliation: Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Chang Jun
authors.initials: CJ
authors.lastname: Bae
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Choi
authors.forename: Song-In
authors.initials: SI
authors.lastname: Kim
authors.forename: Nam-Ho
authors.initials: NH
authors.lastname: Kim
authors.forename: Hee Jae
authors.initials: HJ
authors.lastname: Lee
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Kwon
authors.forename: Wanjoo
authors.initials: W
authors.lastname: Chun