title: The effects of dexmedetomidine on mesenteric arterial occlusion-associated gut ischemia and reperfusion-induced gut and kidney injury in rabbits.
abstract: OBJECTIVE
  We assessed the antioxidant activity of dexmedetomidine (Dex) administered during the ischemic period in a rabbit model of mesenteric ischemia/reperfusion (I/R) injury using biochemical and histopathological methods.
  
  
  METHODS
  A total of 24 male New Zealand white rabbits weighing between 2.5 and 3.0 kg were randomly divided into three groups: the sham group (Group S, n = 8), the I/R group (Group I/R, n = 8), and the I/R plus Dex treatment group (Group Dex, n = 8). In the I/R group, ischemia was achieved with 60 min of mesenteric occlusion. The sham group provided normal basal values. The rabbits in Group I/R were operated to achieve I/R. Group Dex received intravenous Dex 30 min after the commencement of reperfusion (10 μg/kg Dex was infused within 10 min, and then a maintenance dose of 10 μg/kg/h Dex was infused intravenously). For the measurement of tissue malondialdehyde, total antioxidant status, total oxidant status, lipid hydroperoxide levels, superoxide dismutase, catalase, and myeloperoxidase activity levels in the renal tissue samples of animals, the rabbits in each group were sacrificed 3 h after reperfusion. The histopathological examination scores were determined using the intestinal and renal tissues.
  
  
  RESULTS
  The mean malondialdehyde, total oxidant status, myeloperoxidase, and lipid hydroperoxide levels were significantly higher in Group I/R than in Groups S and Dex (P < 0.05). There also were significant decreases in the mean total antioxidant status, catalase, and superoxide dismutase activities in Group I/R compared with Groups S and Dex (P < 0.05). The histopathological examination scores of the intestinal and renal tissues were significantly higher in Group I/R compared with Groups S and Dex (P < 0.05).
  
  
  CONCLUSIONS
  Dex treatment may have biochemical and histopathological benefits by preventing I/R-related cellular damage of intestinal and renal tissues as shown in an experimental mesenteric ischemia model. The preference to use Dex for anesthesia during the mesenteric ischemia procedure may attenuate I/R injury in intestinal and renal tissues.
authors.affiliation: Department of Surgery, School of Medicine, Kars Kafkas University, Pasacayiri 36100, Kars, Turkey.
authors.forename: Kemal
authors.initials: K
authors.lastname: Kiliç
authors.forename: Volkan
authors.initials: V
authors.lastname: Hanci
authors.forename: Sahbettin
authors.initials: S
authors.lastname: Selek
authors.forename: Mahmut
authors.initials: M
authors.lastname: Sözmen
authors.forename: Nergiz
authors.initials: N
authors.lastname: Kiliç
authors.forename: Mehmet
authors.initials: M
authors.lastname: Citil
authors.forename: Derya Arslan
authors.initials: DA
authors.lastname: Yurtlu
authors.forename: B Serhan
authors.initials: BS
authors.lastname: Yurtlu
mesh_terms: D000208:Acute Disease; D058647:Adrenergic alpha-2 Receptor Agonists / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001157:Arterial Occlusive Diseases / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D020927:Dexmedetomidine / Q000494:pharmacology*; D004195:Disease Models, Animal; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008638:Mesenteric Arteries / Q000502:physiology; D016877:Oxidants / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011817:Rabbits; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D016896:Treatment Outcome
chemical_list: D058647:Adrenergic alpha-2 Receptor Agonists; D000975:Antioxidants; D016877:Oxidants; D008315:Malondialdehyde; D020927:Dexmedetomidine; D009195:Peroxidase