title: Characterization of silver nanoparticles synthesized by using marine isolate Streptomyces albidoflavus.
abstract: Silver nanoparticles production by the green chemistry approach was investigated using an isolated marine actinomycetes strain. The isolated strain was identified as Streptomyces albidoflavus based on chemotaxonomic and ribotyping properties. The strain revealed production of silver nanoparticles both extracellular and intracellularly. Surface Plasmon Resonance analysis with the function of time revealed that particle synthesis by this strain is reaction time dependent. The produced particles were spherical shaped and monodispersive in nature and showed a single surface plasmon resonance peak at 410 nm. Size distribution histograms indicated production of 10-40- nm-size nanoparticles with a mean size of 14.5 nm. FT-IR spectra of nanopartilces showed N-H, C-H, and C-N stretching vibrations, denoting the presence of amino acid/ peptide compounds on the surface of silver nanoparticles produced by S. albidoflavus. Synthesized nanoparticles revealed a mean negative zeta potential and electrophoretic mobility of -8.5 mV and -0.000066 cm2/Vs, respectively. The nanoparticles produced were proteinaceous compounds as capping agents with -8.5 mV zeta potential and revealed antimicrobial activity against both Gram-negative and -positive bacterial strains. Owing to their small size, these particles have greater impact on industrial application spectra.
authors.affiliation: Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, 500607, India. prakasam@iict.res.in
authors.forename: Reddy Shetty
authors.initials: RS
authors.lastname: Prakasham
authors.forename: Sudheer Kumar
authors.initials: SK
authors.lastname: Buddana
authors.forename: Sudheer Kumar
authors.initials: SK
authors.lastname: Yannam
authors.forename: Girija Shankar
authors.initials: GS
authors.lastname: Guntuku
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry*; D008969:Molecular Sequence Data; D010316:Particle Size; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*; D012834:Silver / Q000737:chemistry / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver