title: Prognostic value of miR-93 overexpression in resectable gastric adenocarcinomas.
abstract: OBJECTIVE
  MicroRNAs (miRNAs) have been shown to be aberrantly expressed in many human carcinomas. Emerging evidence indicates that miR-93 plays important oncogenic roles in human carcinogenesis and is often up-regulated. However, its relationship with the clinicopathological features and prognosis of human gastric cancer (GC) has yet to be addressed. In this study, we investigate the expression and clinical significance of miR-93 in human gastric cancer.
  
  
  METHODS
  158 patients with gastric adenocarcinoma who had undergone gastrectomy were enrolled. Specimens including the tumor and non-neoplastic were detected for the expression of miR-93 by Real-Time reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, the correlation of miR-93 levels with clinicopathologic variables and prognosis was analyzed.
  
  
  RESULTS
  miR-93 was significantly up-regulated in 128 cases (81%) of the 158 gastric cancer (P < 0.05). Furthermore, the elevated expression of miR-93 was significantly associated with advanced disease stage (P < 0.001), deep invasion level (P < 0.001) and the presence of nodal metastases (P < 0.001). Moreover, gastric cancer patients with high miR-93 expression levels had shorter overall survival (P = 0.001) and disease-free survival (P = 0.006) than that with low miR-93 expression levels.
  
  
  CONCLUSIONS
  miR-93 is highly elevated in gastric cancer, especially in advanced and metastasized gastric cancer, suggesting miR-93 may play critical roles in carcinogenesis of gastric cancer. Overexpression of miR-93 can serve as a novel prognostic marker for gastric cancer.
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Meng
authors.initials: M
authors.lastname: Jiang
authors.forename: Weijie
authors.initials: W
authors.lastname: Yuan
authors.forename: Huihuan
authors.initials: H
authors.lastname: Tang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality*; D000328:Adult; D000368:Aged; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality*; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs