title: Fibrillation of hen egg white lysozyme triggers reduction of copper(II).
abstract: Copper is known to exert diverse effects on the self-association of proteins and has been found in amyloid deposits that are involved in neurodegenerative disease processes. The effects of the metal ion on the protein during fibrillation were investigated by fluorescence, circular dichroism spectroscopy and fluorescence microscopy. We report for the first time, the complete reduction of Cu(II)→Cu(I) in vitro during fibrillation of hen egg white lysozyme at pH 7. This was confirmed by the lack of any signal for Cu(II) in electron paramagnetic resonance spectroscopy and quantification of Cu(I) was achieved by a bathocuproine disulfonate based assay.
authors.affiliation: Department of Chemistry, Indian Institute of Technology, Kharagpur, Kharagpur 721302, India.
authors.forename: Sudeshna
authors.initials: S
authors.lastname: Ghosh
authors.forename: Nitin K
authors.initials: NK
authors.lastname: Pandey
authors.forename: Susmita
authors.initials: S
authors.lastname: Bhattacharya
authors.forename: Anushree
authors.initials: A
authors.lastname: Roy
authors.forename: Swagata
authors.initials: S
authors.lastname: Dasgupta
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry*; D004529:Egg White / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D011485:Protein Binding; D013844:Thiazoles / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D013844:Thiazoles; C009462:thioflavin T; D003300:Copper; C421864:hen egg lysozyme; D009113:Muramidase