title: A study of the glycoantigens of neonatal porcine islet-like cell clusters using a lectin microarray.
abstract: BACKGROUND
  The pig pancreas is considered to be the most suitable source of islets for clinical xenotransplantation. Two types of islet transplantation are: adult pig islets and neonatal porcine islet-like cell clusters (NPCC). However, besides a-Gal expression, differences in glycosylation and xenoantigenicity between both types were not clear so fat to date. In this study, we performed lectin microarray analyses of NPCCs cultured for 1, 5, or 9 days.
  
  
  METHODS
  We studied differences in gycoantigens among several kinds of wild-type NPCCs isolated from 1- to 3-day-old neonatal wild-type pigs (Large White/Landrace × Duroc) and cultured for 1, 5 and 9 days in Ham's 10 in the presence of nicotinamide, using a previously published technique. After sonication and centrifugation, supernatant proteins from each islet were labeled with Cy3, applied to a lectin array and scanned with an SC-Profiler for evaluation using an Array Pro Analyzer.
  
  
  RESULTS
  The overall signals of NPCC at days 5 and 9, showed almost the same values to most lectins, whereas those on day 1 showed differences, suggesting that the NPCC on day 1 contain immature cells that gradually turn to mature NPCCs in culture.
authors.affiliation: Division of Organ Transplantation, Department of Surgery, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nakatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Takama
authors.forename: T
authors.initials: T
authors.lastname: Ueno
authors.forename: C
authors.initials: C
authors.lastname: Inuinaka
authors.forename: S
authors.initials: S
authors.lastname: Takeishi
authors.forename: A
authors.initials: A
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Okitsu
authors.forename: H
authors.initials: H
authors.lastname: Nagashima
authors.forename: M
authors.initials: M
authors.lastname: Fukuzawa
authors.forename: S
authors.initials: S
authors.lastname: Miyagawa
mesh_terms: D000606:Amino Sugars / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D000941:Antigens; D005453:Fluorescence; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D006031:Glycosylation; D007515:Islets of Langerhans / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D040081:Protein Array Analysis; D013552:Swine; D013997:Time Factors
chemical_list: D000606:Amino Sugars; D000941:Antigens; D006023:Glycoproteins; D037102:Lectins; C032233:lactosamine; D005643:Fucose; D008358:Mannose