title: Processing and intracellular localization of rice stripe virus Pc2 protein in insect cells.
abstract: Rice stripe virus (RSV) belongs to the genus Tenuivirus and its genome consists of four single-stranded RNAs encoding seven proteins. Here, we have analyzed the processing and membrane association of Pc2 encoded by vcRNA2 in insect cells. The enhanced green fluorescent protein (eGFP) was fused to the Pc2 and used for the detection of Pc2 fusion proteins. The results showed that Pc2 was cleaved to produce two proteins named Pc2-N and Pc2-C. When expressed alone, either Pc2-N or Pc2-C could transport to the Endoplasmic reticulum (ER) membranes independently. Further mutagenesis studies revealed that Pc2 contained three ER-targeting domains. The results led us to propose a model for the topology of the Pc2 in which an internal signal peptide immediately followed a cleavage site, and two transmembrane regions are contained.
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, People's Republic of China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Zhao
authors.forename: Gaozhan
authors.initials: G
authors.lastname: Zhang
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Dai
authors.forename: Yanling
authors.initials: Y
authors.lastname: Hou
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Liang
authors.forename: Changyong
authors.initials: C
authors.lastname: Liang
mesh_terms: D000818:Animals; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007313:Insecta; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling; D029364:Tenuivirus / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins