title: BM61 of Bombyx mori nucleopolyhedrovirus: its involvement in the egress of nucleocapsids from the nucleus.
abstract: All lepidopteran baculovirus genomes sequenced encode a homolog of the Bombyx mori nucleopolyhedrovirus orf61 gene (Bm61). To determine the role of Bm61 in the baculoviral life cycle, we constructed a Bm61 knockout virus and characterized it in cells. We observed that the Bm61 deletion bacmid led to a defect in production of infectious budded virus (BV). Quantitative PCR analysis of BV in the media culturing the transfected cell indicated that BV was not produced due to Bm61 deletion. Electron microscope analysis showed that in the knockout of Bm61, nucleocapsids were not transported from the nucleus to the cytoplasm. From these results we concluded that BM61 is required in the BV pathway for the egress of nucleocapsids from the nucleus to the cytoplasm.
authors.affiliation: School of Medical Science and Laboratory Medicine, Jiangsu University, China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Shen
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D017353:Gene Deletion; D055786:Gene Knockout Techniques; D017930:Genes, Reporter; D046529:Microscopy, Electron, Transmission; D009685:Nuclear Envelope / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology; D019251:Nucleocapsid / Q000378:metabolism* / Q000648:ultrastructure; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D000077183:Occlusion Body Matrix Proteins; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000077183:Occlusion Body Matrix Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus