title: Transfer of the UAP56 interaction motif of human cytomegalovirus pUL69 to its murine cytomegalovirus homolog converts the protein into a functional mRNA export factor that can substitute for pUL69 during viral infection.
abstract: Nucleocytoplasmic shuttling and interaction with the cellular mRNA export factor UAP56 are prerequisites for the mRNA export activity of human cytomegalovirus (HCMV) pUL69. Although the murine cytomegalovirus homolog pM69 shuttles, it fails to export mRNAs due to its inability to recruit UAP56. However, chimeric proteins comprising pM69 fused to N-terminal pUL69 fragments, including its UAP56 interaction motif, acquire mRNA export activity. Importantly, growth curves of recombinant HCMVs illustrate that such a chimeric protein, but not pM69, substitutes for pUL69 during HCMV infection.
authors.affiliation: Institute for Clinical and Molecular Virology, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Zielke
authors.forename: Nadine
authors.initials: N
authors.lastname: Wagenknecht
authors.forename: Caroline
authors.initials: C
authors.lastname: Pfeifer
authors.forename: Katrin
authors.initials: K
authors.lastname: Zielke
authors.forename: Marco
authors.initials: M
authors.lastname: Thomas
authors.forename: Thomas
authors.initials: T
authors.lastname: Stamminger
mesh_terms: D000818:Animals; D001692:Biological Transport; D003587:Cytomegalovirus / Q000235:genetics / Q000502:physiology; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D006801:Humans; D051379:Mice; D018146:Muromegalovirus / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D011994:Recombinant Proteins; D015534:Trans-Activators; C446019:pUL69 protein, Human herpesvirus 5; C487886:DDX39B protein, human; D053487:DEAD-box RNA Helicases