title: Transcription factor binding to a DNA zip code controls interchromosomal clustering at the nuclear periphery.
abstract: Active genes in yeast can be targeted to the nuclear periphery through interaction of cis-acting "DNA zip codes" with the nuclear pore complex. We find that genes with identical zip codes cluster together. This clustering was specific; pairs of genes that were targeted to the nuclear periphery by different zip codes did not cluster together. Insertion of two different zip codes (GRS I or GRS III) at an ectopic site induced clustering with endogenous genes that have that zip code. Targeting to the nuclear periphery and interaction with the nuclear pore is a prerequisite for gene clustering, but clustering can be maintained in the nucleoplasm. Finally, we find that the Put3 transcription factor recognizes the GRS I zip code to mediate both targeting to the NPC and interchromosomal clustering. These results suggest that zip-code-mediated clustering of genes at the nuclear periphery influences the three-dimensional arrangement of the yeast genome.
authors.affiliation: Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Donna Garvey
authors.initials: DG
authors.lastname: Brickner
authors.forename: Sara
authors.initials: S
authors.lastname: Ahmed
authors.forename: Lauren
authors.initials: L
authors.lastname: Meldi
authors.forename: Abbey
authors.initials: A
authors.lastname: Thompson
authors.forename: Will
authors.initials: W
authors.lastname: Light
authors.forename: Matthew
authors.initials: M
authors.lastname: Young
authors.forename: Taylor L
authors.initials: TL
authors.lastname: Hickman
authors.forename: Feixia
authors.initials: F
authors.lastname: Chu
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Fabre
authors.forename: Jason H
authors.initials: JH
authors.lastname: Brickner
mesh_terms: D015825:Chromosomes, Fungal / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D006032:Glycine-tRNA Ligase / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D022022:Nuclear Pore / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C068399:PUT3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D004247:DNA; C478769:GRS1 protein, S cerevisiae; D006032:Glycine-tRNA Ligase