title: [FTIR analysis of the impact of covalent coupling on the secondary structure of antibody protein].
abstract: The immunolatex was prepared by covalent coupling. FTIR technology combined with substractive spectroscopy, deconvolution, derivation and curve-fitting methods were used to study the structure of the antibody protein on the immunolatex. The result demonstrates that the alpha-helix strcture of antibody increases with the increase in the pH value and the concentration of latex. So it is concluded that covalent coupling has a great impact on the secondary structure of antibody protein.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China. tayiya@126.com
authors.forename: Si-ming
authors.initials: SM
authors.lastname: Yu
authors.forename: Yun-ping
authors.initials: YP
authors.lastname: Peng
authors.forename: Shu-juan
authors.initials: SJ
authors.lastname: Yu
authors.forename: Huan
authors.initials: H
authors.lastname: Lü
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000906:Antibodies