title: Osteogenic differentiation of MC3T3-E1 cells on different titanium surfaces.
abstract: mRNA expressions related to osteogenic differentiation of MC3T3-E1 cells on electro-polished smooth (S), sandblasted small-grit (SSG) and sandblasted large-grit (SLG) surfaces of titanium alloys were investigated in vitro. Gene expression profiles of cells were evaluated using the RT2 Profiler PCR microarray on day 7. Mineralizing tissue-associated proteins, differentiation factors and extracellular matrix enzymes mRNA expressions were measured using Q-PCR. SLG surface upregulated 23 genes over twofolds and downregulated 3 genes when compared to the S surface. In comparison to the SSG surface, at least a twofold increase in 25 genes was observed in the SLG surface. BSP, OCN, OPN, COL I and ALP mRNA expressions increased in the SLG group when compared to the S and the SSG groups. BMP-2, BMP-6 and TGF-β mRNA expressions increased in both the SSG and the SLG surfaces. MMP-2 and MMP-9 mRNA expressions increased as the surface roughness increased. This study demonstrated that surface roughness of titanium implants has a significant effect on cellular behavior and SLG surface apparently increased gene expressions related to osteogenesis when compared to the S and the SSG surfaces.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Selcuk University, Konya 42079, Turkey. sshakki@yahoo.com
authors.forename: Sema S
authors.initials: SS
authors.lastname: Hakki
authors.forename: S Buket
authors.initials: SB
authors.lastname: Bozkurt
authors.forename: Erdogan E
authors.initials: EE
authors.lastname: Hakki
authors.forename: Petek
authors.initials: P
authors.lastname: Korkusuz
authors.forename: Nuhan
authors.initials: N
authors.lastname: Purali
authors.forename: Nurşen
authors.initials: N
authors.lastname: Koç
authors.forename: Muharrem
authors.initials: M
authors.lastname: Timucin
authors.forename: Adnan
authors.initials: A
authors.lastname: Ozturk
authors.forename: Feza
authors.initials: F
authors.lastname: Korkusuz
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D005109:Extracellular Matrix / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008422:Materials Testing; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D010012:Osteogenesis / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D012333:RNA, Messenger; D014025:Titanium