title: Reaction-based two-photon probes for mercury ions: fluorescence imaging with dual optical windows.
abstract: For fluorescent imaging of mercury ions in living species, two-photon probes with dual optical windows are in high demand but remain unexplored. Several dithioacetals were evaluated, and a probe was found, which, upon reaction with mercury species, yielded a two-photon dye; this conversion accompanies ratiometric emission changes with a 97-nm shift, enabling fluorescent imaging of both the probe and mercury ions in cells by one- and two-photon microscopy for the first time.
authors.affiliation: Department of Chemistry and the Center for Electro-Photo Behaviors in Advanced Molecular Systems, Department of Mechanical Engineering and Division of Integrative Biosciences and Biotechnology, POSTECH , San 31, Hyoja-dong, Pohang, 790-784, Republic of Korea.
authors.forename: Alla Sreenivasa
authors.initials: AS
authors.lastname: Rao
authors.forename: Dokyoung
authors.initials: D
authors.lastname: Kim
authors.forename: Taejun
authors.initials: T
authors.lastname: Wang
authors.forename: Ki Hean
authors.initials: KH
authors.lastname: Kim
authors.forename: Sekyu
authors.initials: S
authors.lastname: Hwang
authors.forename: Kyo Han
authors.initials: KH
authors.lastname: Ahn
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D008628:Mercury / Q000032:analysis*; D015394:Molecular Structure; D017785:Photons
chemical_list: D005456:Fluorescent Dyes; D008628:Mercury