title: Reproducible discrimination between gram-positive and gram-negative bacteria using surface enhanced Raman spectroscopy with infrared excitation.
abstract: The on time diagnostics of bacterial diseases is one of the essential steps in the foregoing treatment of such pathogens. Here we sought to present an easy to use and robust method for the discrimination between Gram-positive (Enterococcus faecalis and Streptococcus pyogenes) and Gram-negative (Acinetobacter baumannii and Klebsiella pneumoniae) bacterial genera based on surface enhanced Raman scattering (SERS) spectroscopy. The robustness of our approach lies in the novel method for the production of the SER substrate based on silver nanoparticles and their subsequent re-crystallization in solutions containing high concentrations of chloride ions. The method presented here could be an interesting alternative both to commonly used histochemical approaches and commercial SERS substrates.
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacky University, Slechtitelu 11, 783 71, Olomouc, Czech Republic. robert.prucek@upol.cz
authors.forename: Robert
authors.initials: R
authors.lastname: Prucek
authors.forename: Václav
authors.initials: V
authors.lastname: Ranc
authors.forename: Libor
authors.initials: L
authors.lastname: Kvítek
authors.forename: Aleš
authors.initials: A
authors.lastname: Panáček
authors.forename: Radek
authors.initials: R
authors.lastname: Zbořil
authors.forename: Milan
authors.initials: M
authors.lastname: Kolář
mesh_terms: D016923:Cell Death; D016000:Cluster Analysis; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000166:cytology / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000166:cytology / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013055:Spectrophotometry, Infrared; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D013997:Time Factors