title: Investigation of the network of preferred interactions in an artificial coiled-coil association using the peptide array technique.
abstract: We screened a randomized library and identified natural peptides that bound selectively to a chimeric peptide containing α-, β- and γ-amino acids. The SPOT arrays provide a means for the systematic study of the possible interaction space accessible to the αβγ-chimera. The mutational analysis reveals the dependence of the binding affinities of α-peptides to the αβγ-chimera, on the hydrophobicity and bulkiness of the side chains at the corresponding hydrophobic interface. The stability of the resulting heteroassemblies was further confirmed in solution by CD and thermal denaturation.
authors.affiliation: Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany.
authors.forename: Raheleh
authors.initials: R
authors.lastname: Rezaei Araghi
authors.forename: Carsten C
authors.initials: CC
authors.lastname: Mahrenholz
authors.forename: Rudolf
authors.initials: R
authors.lastname: Volkmer
authors.forename: Beate
authors.initials: B
authors.lastname: Koksch