title: Status of high-risk oncogenic human papillomavirus subtypes harbored in the prepuce of prepubertal boys.
abstract: OBJECTIVE
  To evaluate whether the high-risk human papillomavirus (HPV) subtypes that are strongly related to cervical cancer are harbored in the prepuce of the circumcised tissue of prepubertal boys in the period just before active sexual life.
  
  
  METHODS
  The present study enrolled 30 healthy boys (age range 4-11 years, mean age 8.1 ± 1.6) who underwent a standard circumcision procedure, with hypospadias repair in 3 patients. All prepuceal samples were studied using real-time polymerase chain reaction and grouped according to HPV subtype prevalence as groups 1 (types 16 and 18), 2 (types 31, 33, 45, 52, and 58), and 3 (types 35, 39, 51, 56, 59, 66, and 68).
  
  
  RESULTS
  HPV DNA was reported in 25 (83.3%) of the 30 subjects. All samples showed a negative result for group 2. Although most of the positive findings were for group 3 (25 [83.3%] of 30), a positive result was reported for only 1 subject for group 1 (3.3%).
  
  
  CONCLUSIONS
  The results of the present study have shown that the prepuce harbored the rarest HPV types, including types 35, 39, 51, 56, 59, 66, and 68 in preadolescence boys with a high rate (83%). These findings are in contrast to the common knowledge of HPV prevalence in adults that points to the dominance of HPV subtypes 16 and 18.
authors.affiliation: Department of Urology, Harran University School of Medicine, Sanliurfa, Turkey. veritayhan@yahoo.com
authors.forename: Ayhan
authors.initials: A
authors.lastname: Verit
authors.forename: Fadile Yildiz
authors.initials: FY
authors.lastname: Zeyrek
authors.forename: Cengiz
authors.initials: C
authors.lastname: Mordeniz
authors.forename: Halil
authors.initials: H
authors.lastname: Ciftci
authors.forename: Murat
authors.initials: M
authors.lastname: Savas
mesh_terms: D002648:Child; D002675:Child, Preschool; D015344:DNA Probes, HPV; D052816:Foreskin / Q000821:virology*; D006801:Humans; D008297:Male; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics*; D011627:Puberty; D012307:Risk Factors
chemical_list: D015344:DNA Probes, HPV