title: An integrated, multiparametric flow cytometry chip using "microfluidic drifting" based three-dimensional hydrodynamic focusing.
abstract: In this work, we demonstrate an integrated, single-layer, miniature flow cytometry device that is capable of multi-parametric particle analysis. The device integrates both particle focusing and detection components on-chip, including a "microfluidic drifting" based three-dimensional (3D) hydrodynamic focusing component and a series of optical fibers integrated into the microfluidic architecture to facilitate on-chip detection. With this design, multiple optical signals (i.e., forward scatter, side scatter, and fluorescence) from individual particles can be simultaneously detected. Experimental results indicate that the performance of our flow cytometry chip is comparable to its bulky, expensive desktop counterpart. The integration of on-chip 3D particle focusing with on-chip multi-parametric optical detection in a single-layer, mass-producible microfluidic device presents a major step towards low-cost flow cytometry chips for point-of-care clinical diagnostics.
authors.forename: Xiaole
authors.initials: X
authors.lastname: Mao
authors.forename: Ahmad Ahsan
authors.initials: AA
authors.lastname: Nawaz
authors.forename: Sz-Chin Steven
authors.initials: SC
authors.lastname: Lin
authors.forename: Michael Ian
authors.initials: MI
authors.lastname: Lapsley
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Zhao
authors.forename: J Philip
authors.initials: JP
authors.lastname: McCoy
authors.forename: Wafik S
authors.initials: WS
authors.lastname: El-Deiry
authors.forename: Tony Jun
authors.initials: TJ
authors.lastname: Huang