title: Effects of 50 Hz electromagnetic fields on human epidermal stem cells cultured on collagen sponge scaffolds.
abstract: OBJECTIVE
  This study is to investigate the effects of electromagnetic fields (EMF) on proliferation of epidermal stem cells (ESC), which could present a viable clinical option for skin tissue engineering.
  
  
  METHODS
  The ESC obtained from human foreskin were grafted into type-I three-dimensional collagen sponge scaffolds, and then were exposed with EMF (frequency 50 Hz, intensity 5 mT) for 14 d (30 min per d). Meanwhile, the control group was set under the same conditions without EMF. The effects of EMF on growth and proliferation of ESC were analyzed with staining of hematoxylin and eosin (H&E) and 4',6-diamidino-2-phenylindole (DAPI) under microscope or scanning electron microscope. The data of DAPI staining for 2 d, 7 d, 10 d and 14 d were collected respectively to investigate the cells proliferation.
  
  
  RESULTS
  ESC cultured in collagen sponge scaffolds could be steady grown and EMF could promote ESC proliferation compared with control (P < 0.05).
  
  
  CONCLUSIONS
  EMF could significantly promote proliferation of ESC, which leads to a promising clinical option for skin tissue engineering.
authors.affiliation: Department of Rehabilitation Medicine, Guangdong General Hospital, Institute of Geriatrics, Guangdong Academy of Medical Sciences, Guangzhou, China.
authors.forename: Wen-Fang
authors.initials: WF
authors.lastname: Bai
authors.forename: Ming-Sheng
authors.initials: MS
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
authors.forename: Hong-Xiang
authors.initials: HX
authors.lastname: Zhu
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Xu
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D004560:Electricity; D004574:Electromagnetic Fields; D006801:Humans; D011829:Radiation Dosage; D012867:Skin / Q000166:cytology / Q000254:growth & development* / Q000528:radiation effects*; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D003094:Collagen