title: Immunoassay-based proteome profiling of 24 pancreatic cancer cell lines.
abstract: Pancreatic ductal adenocarcinoma is one of the most deadly forms of cancers, with a mortality that is almost identical to incidence. The inability to predict, detect or diagnose the disease early and its resistance to all current treatment modalities but surgery are the prime challenges to changing the devastating prognosis. Also, relatively little is known about pancreatic carcinogenesis. In order to better understand relevant aspects of pathophysiology, differentiation, and transformation, we analysed the cellular proteomes of 24 pancreatic cancer cell lines and two controls using an antibody microarray that targets 741 cancer-related proteins. In this analysis, 72 distinct disease marker proteins were identified that had not been described before. Additionally, categorizing cancer cells in accordance to their original location (primary tumour, liver metastases, or ascites) was made possible. A comparison of the cells' degree of differentiation (well, moderately, or poorly differentiated) resulted in unique marker sets of high relevance. Last, 187 proteins were differentially expressed in primary versus metastatic cancer cells, of which the majority is functionally related to cellular movement.
authors.affiliation: Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum (DKFZ), Im Neuenheimer Feld 580, Heidelberg, Germany. m.alhamdani@dkfz.de
authors.forename: Mohamed Saiel Saeed
authors.initials: MS
authors.lastname: Alhamdani
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Youns
authors.forename: Malte
authors.initials: M
authors.lastname: Buchholz
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Gress
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: Beckers
authors.forename: Daniel
authors.initials: D
authors.lastname: Maréchal
authors.forename: Andrea
authors.initials: A
authors.lastname: Bauer
authors.forename: Christoph
authors.initials: C
authors.lastname: Schröder
authors.forename: Jörg D
authors.initials: JD
authors.lastname: Hoheisel
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry; D021441:Carcinoma, Pancreatic Ductal / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D009363:Neoplasm Proteins / Q000032:analysis* / Q000737:chemistry; D010190:Pancreatic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D020543:Proteome