title: Evaluation of the solid phase competition ELISA for detecting antibodies against the six foot-and-mouth disease virus non-O serotypes.
abstract: The solid-phase competition ELISA (SPCE) has been evaluated in both screening and titration assay formats for detecting antibodies against foot-and-mouth disease virus (FMDV) for the six non-O serotypes A, C, SAT 1, SAT 2, SAT 3 and Asia 1. Cut-off values were determined as a percentage inhibition of 40 for the SAT serotypes and 50 for serotypes A, C and Asia 1, which gave rise to specificity values ranging from 99.41% to 99.9% for the different serotypes. The relative sensitivity between the SPCE and LPBE/virus neutralisation test was 100%/109%. Antiserum titres derived by the SPCE for samples of serotypes O, A(22) and Asia 1 were more than 11, 1 and 5 times of those determined by virus neutralisation test, respectively. This study indicated that the non-type O SPCEs have sufficient sensitivities and specificities for use as serological diagnostic tests for the qualitative and quantitative detection of antibodies against FMDV.
authors.affiliation: Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 0NF, United Kingdom. yanmin.li@iah.ac.uk
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Li
authors.forename: Kate G
authors.initials: KG
authors.lastname: Swabey
authors.forename: Debi
authors.initials: D
authors.lastname: Gibson
authors.forename: Phil J
authors.initials: PJ
authors.lastname: Keel
authors.forename: Pip
authors.initials: P
authors.lastname: Hamblin
authors.forename: Ginette
authors.initials: G
authors.lastname: Wilsden
authors.forename: Mandy
authors.initials: M
authors.lastname: Corteyn
authors.forename: Nigel P
authors.initials: NP
authors.lastname: Ferris
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000737:chemistry; D000914:Antibodies, Viral / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D001667:Binding, Competitive; D002417:Cattle / Q000276:immunology; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep / Q000276:immunology; D012757:Sheep Diseases / Q000276:immunology / Q000821:virology; D013552:Swine / Q000276:immunology; D013553:Swine Diseases / Q000276:immunology / Q000821:virology; D014611:Vaccination
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral