title: Effect of Buddleia flavonoids drug-containing plasma on the expression of STAT1 phosphoprotein in lacrimal gland epithelial cells in vitro.
abstract: OBJECTIVE
  To explore the effect of Buddleia flavonoids drug-containing plasma and androgen receptor (AR) blocker on the expression of STAT1 phosphoprotein.
  
  
  METHODS
  In vitro lacrimal gland epithelial cells were cultivated with H(2)O(2) to establish the dry eye apoptosis state. Blank plasma group, Buddleia officinalis plasma total flavonoids interfere with drug-containing group, and the intervention group of testosterone propionate were set. The expressions of STAT1 phosphoprotein of each group were observed by Western blot. AR blocker flutamide was used to explore the intended androgen effect of Buddleia flavonoids.
  
  
  RESULTS
  After the intervention of drug-containing plasma, the expression of STAT1 phosphoprotein in Buddleja officinalis drug-containing plasma intervention group (0.353±0.494) and testosterone propionate intervention group (0.502±0.036) were enhanced and the differences between the two groups were significant (P<0.01). After using AR blocker, the expression of STAT1 phosphoprotein in each group (0.268±0.061, 0.283±0.106, 0.213±0.071) had no difference.
  
  
  CONCLUSIONS
  Buddleja officinalis drug-containing plasma total flavonoids can promote the expression of STAT1 phosphorylation.
authors.affiliation: Department of Ophthalmology, Second Affiliated Hospital of Guiyang University of Traditional Chinese Medicine, Guiyang 550003, Guizhou Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Peng
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Yao
authors.forename: Quan-Long
authors.initials: QL
authors.lastname: Wu
authors.forename: Dian
authors.initials: D
authors.lastname: Li