title: Successful collection and engraftment of autologous peripheral blood progenitor cells in poorly mobilized patients receiving high-dose granulocyte colony-stimulating factor.
abstract: BACKGROUND
  Granulocyte Colony-Stimulating Factor (G-CSF) alone or in conjunction with chemotherapy is commonly used to mobilize hematopoietic progenitor cells (HPC) into peripheral blood for progenitor cell harvest for autologous HPC transplantation. However, in up to 30% of patients, HPC are not effectively mobilized. In this study, we report the efficacy and safety profiles of a mobilization strategy using high-dose (up to 36 μg/kg) G-CSF in poorly mobilized patients.
  
  
  METHODS
  Retrospective medical record reviews were performed for 392 patients who underwent autologous peripheral blood progenitor cell collection. A total of 56 patients were given high-dose G-CSF due to very ineffective mobilization and 35 of these patients underwent autologous HPC transplantation. The efficacy of mobilization, apheresis collection, and infusion were reviewed and analyzed.
  
  
  RESULTS
  More than 2.5 × 10(6) CD34/Kg were collected in 88% of patients (49 of 56) who were placed on high-dose G-CSF due to very ineffective mobilization. Of the 35 patients who underwent HPC transplantation using the progenitor cells that were mobilized with high-dose G-CSF due to very ineffective mobilization, all had rapid and complete neutrophil and platelet engraftment comparable with good mobilizers.
  
  
  CONCLUSIONS
  We conclude that collection of HPC using hyperstimulation with G-CSF is an effective alternative approach for HPC harvest for poorly mobilized patients.
authors.affiliation: Section of Medical Oncology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Dennis L
authors.initials: DL
authors.lastname: Cooper
authors.forename: Maria
authors.initials: M
authors.lastname: Proytcheva
authors.forename: Erin
authors.initials: E
authors.lastname: Medoff
authors.forename: Stuart E
authors.initials: SE
authors.lastname: Seropian
authors.forename: Edward L
authors.initials: EL
authors.lastname: Snyder
authors.forename: Diane S
authors.initials: DS
authors.lastname: Krause
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000096:biosynthesis; D001792:Blood Platelets / Q000166:cytology; D002648:Child; D002675:Child, Preschool; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology; D012189:Retrospective Studies; D013234:Stem Cells / Q000166:cytology*
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor