title: [Survey on the transmission of HIV drug resistance in Kunming, Yunnan province in 2010].
abstract: OBJECTIVE
  To study the HIV drug resistance (HIVDR) transmission in Kunming city of Yunnan province in 2010.
  
  
  METHODS
  Referring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS) set by WHO, 62 plasma samples of recently reported HIV-infected individuals who were older than 25 years of age, were collected from January to August 2010. Genotyping of pol genetic mutations associated with HIVDR with reverse transcriptional PCR was performed and the prevalence of HIV-1 drug resistance transmission was evaluated.
  
  
  RESULTS
  Of the 62 plasma samples, 54 were successfully sequenced and genotyped on pol sequence. Based on the pol sequences, HIV subtypes including CRF08_BC (53.2%), CRF07_BC (25.5%), CRF01_AE (19.1%) and C (2.1%) were identified. According to the time of sampling, the first 47 sequenced samples were used for drug resistance prevalence analysis. A protease inhibitor (PI) relative mutation was found in one sample. Based on the WHO standard, the prevalence of transmitted HIV-1 drug resistance was < 5%.
  
  
  CONCLUSIONS
  HIV-1 drug resistant strains transmission was still catalogued as low prevalence level in Kunming. To prevent the increase of HIVDR prevalence, normative treatment and scientific management to AIDS patients seemed to be quite important.
authors.affiliation: Yunnan Center for Disease Control and Prevention, Kunming 650022, China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Ma
authors.forename: Cheng-xia
authors.initials: CX
authors.lastname: Chu
authors.forename: Hui
authors.initials: H
authors.lastname: Xing
authors.forename: Yan-sheng
authors.initials: YS
authors.lastname: Xu
authors.forename: Ying-zhen
authors.initials: YZ
authors.lastname: Su
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Hui-chao
authors.initials: HC
authors.lastname: Chen
authors.forename: Hong-bing
authors.initials: HB
authors.lastname: Luo
authors.forename: Man-hong
authors.initials: MH
authors.lastname: Jia
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
mesh_terms: D000328:Adult; D002681:China; D024882:Drug Resistance, Viral; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans