title: Enhanced antiviral activity of acyclovir loaded into nanoparticles.
abstract: The activity of antivirals can be enhanced by their incorporation in nanoparticulate delivery systems. Peculiar polymeric nanoparticles, based on a β-cyclodextrin-poly(4-acryloylmorpholine) monoconjugate (β-CD-PACM), are proposed as acyclovir carriers. The experimental procedure necessary to obtain the acyclovir-loaded nanoparticles using the solvent displacement preparation method will be described in this chapter. Fluorescent labeled nanoparticles are prepared using the same method for cellular trafficking studies. The biocompatibility assays necessary to obtain safe nanoparticles are reported. Section 4 of this chapter describes the assessment of the antiviral activity of the acyclovir-loaded nanoparticles.
authors.affiliation: Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Torino, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Cavalli
authors.forename: Manuela
authors.initials: M
authors.lastname: Donalisio
authors.forename: Agnese
authors.initials: A
authors.lastname: Bisazza
authors.forename: Andrea
authors.initials: A
authors.lastname: Civra
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Ranucci
authors.forename: Paolo
authors.initials: P
authors.lastname: Ferruti
authors.forename: David
authors.initials: D
authors.lastname: Lembo
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000212:Acyclovir / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D003167:Complement Activation / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry*; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000187:drug effects; D013499:Surface Properties; D046509:Tissue Culture Techniques; D014709:Vero Cells; D019562:Viral Load; D010948:Viral Plaque Assay; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D000998:Antiviral Agents; D053769:Nanocapsules; D017382:Reactive Oxygen Species; D047392:beta-Cyclodextrins; C015460:poly(acryloylmorpholine); D000212:Acyclovir