title: [Effect of jinmaitong capsule on inducible nitric oxide synthase and nitrotyrosine in diabetic rats].
abstract: OBJECTIVE
  To observe the effect of Jinmaitong capsule (JMT), a compound traditional Chinese medicine, on expressions of inducible nitric oxide synthase (iNOS) and nitro tyrosine (NT) protein in streptozocin-induced diabetic (STZ-DM) rats.
  
  
  METHODS
  Intraperitoneal injection of streptozocin in rats to establish a model. STZ-DM rats were randomly divided into the model control group (distilled water), the small-dose JMT group (JMT at dose of 0.45 g x kg(-1) x d(-1)), the medium-dose JMT group (JMT at dose of 0.88 g x kg(-1) x d(-1)), the large-dose JMT group (JMT at dose of 1.75 g x kg(-1) x d(-1)) and Vitamin C group (VC at dose of 0.05 g x kg(-1) x d(-1)). Ten normal rats with matching weight and age were selected as the normal control group (distilled water). After intragastric administration for 16 weeks, the expressions of iNOS and NT in sciatic nerve were detected by the immunohistochemistry method.
  
  
  RESULTS
  The expression levels of iNOS and NT protein in diabetic rats were higher than those in normal rats (P<0.05, P<0.01). Compared with the model group, the levels of iNOS and NT protein in JMT and VC groups were significantly decreased (P<0.05, P<0.01). Particularly, the medium-dose JMT group showed a better effect than the VC group (P<0.05, P<0.01).
  
  
  CONCLUSIONS
  JMT could down-regulate the expressions of iNOS and NT protein of sciatic nerve in diabetic rats.
authors.affiliation: Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Liang
authors.forename: Puyan
authors.initials: P
authors.lastname: Wang
authors.forename: Bowu
authors.initials: B
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Huang
authors.forename: Yandong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001786:Blood Glucose; D001835:Body Weight / Q000187:drug effects; D002214:Capsules; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D003929:Diabetic Neuropathies / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D002214:Capsules; D004365:Drugs, Chinese Herbal; C530084:xinmaitong; C002744:3-nitrotyrosine; D014443:Tyrosine; D052247:Nitric Oxide Synthase Type II