title: Partial purification of a Bacillus licheniformis levansucrase producing levan with antitumor activity.
abstract: The extracellular fructosyltransferase (FTase) of a novel strain of Bacillus licheniformis capable of producing fructooligosaccharides (FOS) and a polysaccharide type levan was obtained and partially purified. The purification was achieved by ammonium sulfate precipitation, DEAE cellulose and gel filtration chromatographies. The enzyme was partially purified as determined by SDS-PAGE, and the specific activity reached was 67.5, representing a purification factor of 177 and yield of 40%. Levan was isolated from the cultures of B. licheniformis. The levan was composed mainly of fructose residues when analyzed by TLC after acid hydrolysis and NMR analysis. In a previous study, the levan produced exhibited a hypoglycemiant activity. The present paper deals with the study of the antitumor and anti-cytotoxic effect of levan produced by B. licheniformis strain. In the in vitro antitumor activity test of levan against some tumor cell lines, relatively the significantly high activity was observed against the HepG(2).
authors.affiliation: Biochemistry Laboratory, Faculty of Sciences of Sfax, PB 802, 3018 Sfax, Tunisia. imenbiologie@yahoo.fr
authors.forename: Imen
authors.initials: I
authors.lastname: Dahech
authors.forename: Karima Srih
authors.initials: KS
authors.lastname: Belghith
authors.forename: Hafedh
authors.initials: H
authors.lastname: Belghith
authors.forename: Hafedh
authors.initials: H
authors.lastname: Mejdoub
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D001407:Bacillus / Q000201:enzymology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005630:Fructans / Q000138:chemical synthesis / Q000494:pharmacology*; D006602:Hexosyltransferases / Q000302:isolation & purification* / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D009844:Oligosaccharides / Q000138:chemical synthesis
chemical_list: D000970:Antineoplastic Agents; D005630:Fructans; D009844:Oligosaccharides; C116580:fructooligosaccharide; C072599:levan; D006602:Hexosyltransferases; C021513:levansucrase