title: Magnetic capture hybridization of Batrachochytrium dendrobatidis genomic DNA.
abstract: We hybridized biotinylated probes that anneal at multiple locations throughout the Batrachochytrium dendrobatidis (Bd) genome to selectively capture Bd genomic DNA (gDNA) by binding the probe-gDNA complex to streptavidin coated magnetic beads. We then whole genome amplified the captured gDNA. This method extends the usefulness of field-collected swabs for downstream PCR-based genomic applications.
authors.affiliation: Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY 14853, USA. dr376@cornell.edu
authors.forename: David
authors.initials: D
authors.lastname: Rodriguez
authors.forename: Ana V
authors.initials: AV
authors.lastname: Longo
authors.forename: Kelly R
authors.initials: KR
authors.lastname: Zamudio
mesh_terms: D000818:Animals; D001001:Anura / Q000382:microbiology; D001710:Biotin / Q000737:chemistry; D008411:Chytridiomycota / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D016681:Genome, Fungal; D055767:Immobilized Proteins / Q000737:chemistry; D060328:Magnetic Phenomena; D008863:Microspheres; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization; D012867:Skin / Q000382:microbiology; D019809:Streptavidin / Q000737:chemistry
chemical_list: D015342:DNA Probes; D004271:DNA, Fungal; D055767:Immobilized Proteins; D001710:Biotin; D019809:Streptavidin