title: [Isolation and purification of Mn-peroxidase from Azospirillum brasilense Sp245].
abstract: Homogenous Mn-peroxidase of a 26-fold purity grade was isolated from a culture of Azospirillum brasilense Sp245 cultivated on a medium containing 0.1 mM pyrocatechol. The molecular weight of the enzyme is 43 kD as revealed by electrophoresis in SDS-PAAG. It was shown that the use of pyrocatechol and 2,2'-azino-bis(3-ethylbenzotiazoline-6-sulfonate) at concentrations of 0.1 and I mM as inductors increased the Mn-peroxidase activity by a factor of 3.
authors.forename: M A
authors.initials: MA
authors.lastname: Kupriashina
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Selivanov
authors.forename: V E
authors.initials: VE
authors.lastname: Nikitina
mesh_terms: D015806:Azospirillum brasilense / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D052160:Benzothiazoles / Q000494:pharmacology; D002396:Catechols / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D007700:Kinetics; D008970:Molecular Weight; D010544:Peroxidases / Q000096:biosynthesis* / Q000302:isolation & purification; D012988:Soil Microbiology; D013451:Sulfonic Acids / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D052160:Benzothiazoles; D002396:Catechols; D003470:Culture Media; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D010544:Peroxidases; C051129:manganese peroxidase; C034221:catechol