title: Papuamine and haliclonadiamine, obtained from an Indonesian sponge Haliclona sp., inhibited cell proliferation of human cancer cell lines.
abstract: The extract of an Indonesian marine sponge Haliclona sp. showed potent cytotoxicity against human solid cancer cell lines, MCF-7 (breast), LNCap (prostate), Caco-2 (colon) and HCT-15 (colon) cells. Study on nuclear morphological changes and flow cytometric analysis suggested that the component(s) in the extract would induce an apoptosis to these cancer cells. Bioassay-guided isolation yielded two pentacyclic alkaloids, papuamine (1) and haliclonadiamine (2), which inhibited cell proliferation of six human cancer cell lines with IC50 values of 0.93-1.50 and 1.00-4.44 µM, respectively. Compounds 1 and 2 accumulated lymphoma U937 cells at sub-G1 phase and induced a condensation of chromatin and fragmentation of nucleus.
authors.affiliation: Faculty of Pharmaceutical Sciences, Tohoku Pharmaceutical University, Sendai , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Defny S
authors.initials: DS
authors.lastname: Wewengkang
authors.forename: Syu-Ichi
authors.initials: S
authors.lastname: Kanno
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Henki
authors.initials: H
authors.lastname: Rotinsulu
authors.forename: Remy E P
authors.initials: RE
authors.lastname: Mangindaan
authors.forename: Michio
authors.initials: M
authors.lastname: Namikoshi
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D011161:Porifera / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; C585612:haliclonadiamine; C585611:papuamine