title: Effects of human exogenous DNA on production of perforin-containing CD8+ cytotoxic lymphocytes in laboratory setting and clinical practice.
abstract: We investigated the influence of Panagen DNA preparations on laboratory animals and IFN-induced human dendritic cells, as well as analyzed the data from a phase II clinical trial in the therapy of breast cancer. It was shown that this treatment resulted in increased number of CD8+/perforin+ T cells in peripheral lymphoid organs of experimental animals, in mixed lymphocyte culture population and in peripheral blood of breast cancer patients. Moreover, we demonstrated that when Panagen DNA preparations are used in combination with the standard FAC-based breast cancer therapies, non-specific immune response activity remains at the same levels as observed prior to therapy, whereas in FAC-placebo patients, non-specific immunity is greatly diminished.
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Alyamkina
authors.forename: Olga Y
authors.initials: OY
authors.lastname: Leplina
authors.forename: Alexandr A
authors.initials: AA
authors.lastname: Ostanin
authors.forename: Elena R
authors.initials: ER
authors.lastname: Chernykh
authors.forename: Valeriy P
authors.initials: VP
authors.lastname: Nikolin
authors.forename: Nelly A
authors.initials: NA
authors.lastname: Popova
authors.forename: Anastasia S
authors.initials: AS
authors.lastname: Proskurina
authors.forename: Tatiana S
authors.initials: TS
authors.lastname: Gvozdeva
authors.forename: Evgenia V
authors.initials: EV
authors.lastname: Dolgova
authors.forename: Konstantin E
authors.initials: KE
authors.lastname: Orishchenko
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Rogachev
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Sidorov
authors.forename: Nikolay A
authors.initials: NA
authors.lastname: Varaksin
authors.forename: Tatiana G
authors.initials: TG
authors.lastname: Ryabicheva
authors.forename: Sergey S
authors.initials: SS
authors.lastname: Bogachev
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Shurdov
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D017322:Clinical Trials, Phase II as Topic; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004247:DNA / Q000494:pharmacology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D054353:Perforin / Q000096:biosynthesis / Q000276:immunology*
chemical_list: D054353:Perforin; D004247:DNA