title: Generation of nitric oxide and reactive oxygen species by neutrophils and monocytes from septic patients and association with outcomes.
abstract: In this study, our aims were to evaluate the reactive oxygen species (ROS) and nitric oxide (NO) generation by monocytes and neutrophils from septic patients and to correlate their levels with clinical outcomes. Forty-nine septic patients and 19 healthy volunteers were enrolled in the study. The ROS and NO production was quantified in monocytes and neutrophils in whole blood by flow cytometry, constitutively, and after stimulation with Staphylococcus aureus and Pseudomonas aeruginosa. Nitric oxide production by monocytes was higher in septic patients compared with healthy volunteers for all conditions and by neutrophils at baseline, and ROS generation in monocytes and neutrophils was higher in septic patients than in healthy volunteers for all conditions. Nitric oxide production by monocytes and neutrophils was decreased at day 7 compared with that at admission (day 0) in survivors at baseline and after stimulation with S. aureus. Reactive oxygen species production by the monocytes and neutrophils was decreased in survivors at day 7 compared with day 0 under all conditions, except by neutrophils at baseline. No difference was found in NO and ROS generation by monocytes and neutrophils between day 7 and day 0 in nonsurvivors. Generation of NO and ROS by neutrophils and monocytes is increased in septic patients, and their persistence is associated with poor outcome.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Hospital São Paulo, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Sidnéia Sousa
authors.initials: SS
authors.lastname: Santos
authors.forename: Milena Karina Colo
authors.initials: MK
authors.lastname: Brunialti
authors.forename: Otelo
authors.initials: O
authors.lastname: Rigato
authors.forename: Flavia Ribeiro
authors.initials: FR
authors.lastname: Machado
authors.forename: Eliezer
authors.initials: E
authors.lastname: Silva
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Salomao
mesh_terms: D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D011379:Prognosis; D011550:Pseudomonas aeruginosa / Q000276:immunology; D017382:Reactive Oxygen Species / Q000097:blood*; D016897:Respiratory Burst / Q000276:immunology; D018805:Sepsis / Q000097:blood* / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D015415:Biomarkers; D017382:Reactive Oxygen Species; D009569:Nitric Oxide