title: Effects of genotype and environment on the contents of betaine, choline, and trigonelline in cereal grains.
abstract: This study examined the environmental and genetic variation in methyl donor contents and compositions of 200 cereal genotypes. Glycine betaine, choline, and trigonelline contents were determined by (1)H NMR, and significant differences were observed between cereal types (G) and across harvesting years and growing locations (E). Glycine betaine was the most abundant methyl donor in all of the 200 lines grown on a single site, and concentrations ranged from 0.43 ± 0.09 mg/g dm in oats to 2.57 ± 0.25 mg/g dm in diploid Einkorn varieties. In bread wheat genotypes there was a 3-fold difference in glycine betaine content. Choline contents, in the same lines, were substantially lower, and mean concentrations ranged from 0.17 mg/g dm in oats to 0.27 mg/g dm in durum wheat. Trigonelline was by far the least abundant of the methyl donors studied. Despite this, however, there were large differences between cereal types. Twenty-six wheat genotypes were grown in additional years at four European locations. The average glycine betaine content was highest in grains grown in Hungary and lowest in those grown in the United Kingdom. Across the six environments, there was a 3.8-fold difference in glycine betaine content. Glycine betaine levels, although moderately heritable (0.36), were found to be the most susceptible to the environmental conditions. Free choline concentrations were less variable across genotypes, but heritability of this component was the lowest of all methyl donor components (0.25) and showed a high G × E interaction. Trigonelline showed the most variation due to genotype. Heritability of this metabolite was the highest (0.59), but given that it is at a very low concentration in wheat, it is probably not attractive to plant breeders.
authors.affiliation: Plant Science Department, Rothamsted Research, West Common, Harpenden Herts AL5 2JQ, United Kingdom.
authors.forename: Delia-Irina
authors.initials: DI
authors.lastname: Corol
authors.forename: Catherine
authors.initials: C
authors.lastname: Ravel
authors.forename: Mariann
authors.initials: M
authors.lastname: Raksegi
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Bedo
authors.forename: Gilles
authors.initials: G
authors.lastname: Charmet
authors.forename: Michael H
authors.initials: MH
authors.lastname: Beale
authors.forename: Peter R
authors.initials: PR
authors.lastname: Shewry
authors.forename: Jane L
authors.initials: JL
authors.lastname: Ward
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000378:metabolism; D001622:Betaine / Q000032:analysis* / Q000378:metabolism; D002794:Choline / Q000032:analysis* / Q000378:metabolism; D017753:Ecosystem; D002523:Edible Grain / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004777:Environment; D005838:Genotype; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; C009560:trigonelline; D001622:Betaine; D002794:Choline