title: Isolation of porcine pancreatic islets for xenotransplantation.
abstract: This chapter deals with a technique for isolating intact islets of Langerhans from the pig pancreas based on our experience performing approximately 750 isolations. The procedure we describe involves identification of an optimal donor pancreas, purification and in vitro culture of islets, diabetes induction in recipients, and transplantation of islets and their immunomodulation. Besides the sophistication of the technical equipment employed, the major factors influencing the isolation outcome are the pig breed, the number and morphology of the islets in the donor pancreas, the quality of the collagenase/neutral protease, and the skill of the team members.
authors.affiliation: Experimental Transplantation Immunology and Experimental Surgery, Clinic of General, Visceral, Vascular, and Pediatric Surgery, University of Wuerzburg Hospital, Würzburg, Germany. ulrichs@chirurgie.uni-wuerzburg.de
authors.forename: Karin
authors.initials: K
authors.lastname: Ulrichs
authors.forename: Sibylle
authors.initials: S
authors.lastname: Eber
authors.forename: Bianca
authors.initials: B
authors.lastname: Schneiker
authors.forename: Sabine
authors.initials: S
authors.lastname: Gahn
authors.forename: Armin
authors.initials: A
authors.lastname: Strauß
authors.forename: Vasiliy
authors.initials: V
authors.lastname: Moskalenko
authors.forename: Irina
authors.initials: I
authors.lastname: Chodnevskaja
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D003921:Diabetes Mellitus, Experimental / Q000628:therapy; D005260:Female; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D051379:Mice; D051381:Rats; D013552:Swine; D046509:Tissue Culture Techniques; D020858:Tissue and Organ Harvesting / Q000379:methods; D014183:Transplantation, Heterologous / Q000379:methods*