title: Impact of coculture with ischemic preconditioned hepatocellular carcinoma cell line (Hep-G2) cells on insulin secreting function of rat insulin-secreting cell line (RIN-5F) cells.
abstract: BACKGROUND
  Although Islet cell isolation and culture have been well developed, there has been little progress to prolong transplanted islet survival. Hepatic ischemia and insufficient neovascularization of islets are considered to be the barriers to long-term survival, Hepatocytes that survive ischemic injury have been reported to protect themeslves and regenerate using the IL-6 interleukin 6 and STAT3 pathways.
  
  
  METHODS
  The hepatocellular carcinoma (Hep-G2) cell line preconditioned for 0, 2, 4, 6, and 24 hours in a hypoxic chamber, was cocultured with rat insulin-secreting celline (RIN-5F) cells. We measured cell viabilities, insulin secretion, and p-STAT3, IL-6, and NF-κB levels.
  
  
  RESULTS
  Cocultured Hep-G2 and RIN-5F cells aggregated to form spheroids. Viabilities of Hep-G2 cells were no different after various ischemic preconditioning times, but insulin secretion increased in a time-dependent fashion with preconditioning. Western blotting showed p-STAT3, NF-κB, and IL-6 levels to increase with preconditioning time.
  
  
  CONCLUSIONS
  The IL-6/STAT3 pathway of Hep-G2 cells after ischemic injury showed beneficial effects on insulin secretion of RIN-5f cells cocultured with themselves.
authors.affiliation: The Research Institute for Transplantation, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: D J
authors.initials: DJ
authors.lastname: Joo
authors.forename: J Y
authors.initials: JY
authors.lastname: Kim
authors.forename: J I
authors.initials: JI
authors.lastname: Lee
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: Y H
authors.initials: YH
authors.lastname: Fang
authors.forename: J H
authors.initials: JH
authors.lastname: Jeong
authors.forename: M S
authors.initials: MS
authors.lastname: Kim
authors.forename: K H
authors.initials: KH
authors.lastname: Huh
mesh_terms: D000818:Animals; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D002450:Cell Communication; D015687:Cell Hypoxia; D002470:Cell Survival; D018920:Coculture Techniques; D056945:Hep G2 Cells; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000378:metabolism* / Q000473:pathology; D015850:Interleukin-6 / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D050796:STAT3 Transcription Factor / Q000378:metabolism; D018874:Spheroids, Cellular; D013997:Time Factors
chemical_list: C508600:IL6 protein, human; D007328:Insulin; D015850:Interleukin-6; D016328:NF-kappa B; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human