title: Development of a dedicated peptide tandem mass spectral library for conservation science.
abstract: In recent years, the use of liquid chromatography tandem mass spectrometry (LC-MS/MS) on tryptic digests of cultural heritage objects has attracted much attention. It allows for unambiguous identification of peptides and proteins, and even in complex mixtures species-specific identification becomes feasible with minimal sample consumption. Determination of the peptides is commonly based on theoretical cleavage of known protein sequences and on comparison of the expected peptide fragments with those found in the MS/MS spectra. In this approach, complex computer programs, such as Mascot, perform well identifying known proteins, but fail when protein sequences are unknown or incomplete. Often, when trying to distinguish evolutionarily well preserved collagens of different species, Mascot lacks the required specificity. Complementary and often more accurate information on the proteins can be obtained using a reference library of MS/MS spectra of species-specific peptides. Therefore, a library dedicated to various sources of proteins in works of art was set up, with an initial focus on collagen rich materials. This paper discusses the construction and the advantages of this spectral library for conservation science, and its application on a number of samples from historical works of art.
authors.affiliation: Royal Institute for Cultural Heritage (KIK/IRPA), Jubelpark 1, B-1000 Brussels, Belgium. wim.fremout@kikirpa.be
authors.forename: Wim
authors.initials: W
authors.lastname: Fremout
authors.forename: Maarten
authors.initials: M
authors.lastname: Dhaenens
authors.forename: Steven
authors.initials: S
authors.lastname: Saverwyns
authors.forename: Jana
authors.initials: J
authors.lastname: Sanyova
authors.forename: Peter
authors.initials: P
authors.lastname: Vandenabeele
authors.forename: Dieter
authors.initials: D
authors.lastname: Deforce
authors.forename: Luc
authors.initials: L
authors.lastname: Moens
mesh_terms: D000269:Adhesives / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001530:Belgium; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D049672:History, 19th Century; D008969:Molecular Sequence Data; D010150:Paint / Q000032:analysis* / Q000266:history*; D010151:Paintings / Q000266:history*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D011817:Rabbits; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000269:Adhesives; D019151:Peptide Library; D010455:Peptides; D011506:Proteins