title: Novel quercetin glucosides from Helminthostachys zeylanica root and acceleratory activity of melanin biosynthesis.
abstract: Two novel quercetin glucosides, namely 4'-O-β-D-glucopyranosyl-quercetin-3-O-β-D-glucopyranosyl-(1→4)-β-D-glucopyranoside (compound 1) and 4'-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-quercetin-3-O-β-D-glucopyranosyl-(1→4)-β-D-glucopyranoside (compound 2), were isolated from Helminthostachys zeylanica root 50 % EtOH extract. Structural analysis of isolated compounds was achieved mainly by 600 MHz and 800 MHz NMR, UPLC-TOFMS and GC-MS. Of the two quercetin glucosides, compound 1 showed a high melanogenic acceleratory effect, 2.7 times higher than control, at 10 μM concentration in murine B16 melanoma cells, with no cytotoxic effect.
authors.affiliation: Faculty of Applied Biological Science, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Kosei
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Tohru
authors.initials: T
authors.lastname: Mitsunaga
authors.forename: Irmanida
authors.initials: I
authors.lastname: Batubara
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D029624:Ferns / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008543:Melanins / Q000378:metabolism*; D051379:Mice; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry*
chemical_list: D005960:Glucosides; D008543:Melanins; D011794:Quercetin