title: Cryopreservation does not  alter karyotype, multipotency, or NANOG/SOX2 gene expression of amniotic fluid  mesenchymal stem cells.
abstract: Cryopreservation  of mesenchymal stem cells from amniotic fluid is of clinical importance, as  these cells can be harvested during the prenatal period and stored for use in  treatments. We examined the behavior of mesenchymal stem cells from human  amniotic fluid in culture that had been subjected to cryopreservation. We  assessed chromosomal stability through karyotype analysis, determined whether  multipotent capacity (differentiation into adipogenic, chondrogenic, and  osteogenic cells) is maintained, and analyzed SOX2 and NANOG expression after  thawing. Five amniotic fluid samples were cryopreserved for 150 days. No  chromosomal aberrations were observed. The expression levels of NANOG and SOX2  also were quite similar before and after cryopreservation. Capacity for  differentiation into adipogenic, chondrogenic, and osteogenic tissues also  remained the same. We conclude that cryopreservation of amniotic fluid does not  alter karyotype, NANOG/SOX2 gene expression, or multipotent capacity of stem  cells that have been collected from amniotic fluid during pregnancy.
authors.affiliation: Instituto Hermes Pardini, Belo Horizonte, MG, Brasil.
authors.forename: P C
authors.initials: PC
authors.lastname: Angelo
authors.forename: A C S
authors.initials: AC
authors.lastname: Ferreira
authors.forename: V D
authors.initials: VD
authors.lastname: Fonseca
authors.forename: S P
authors.initials: SP
authors.lastname: Frade
authors.forename: C S
authors.initials: CS
authors.lastname: Ferreira
authors.forename: F S V
authors.initials: FS
authors.lastname: Malta
authors.forename: A K
authors.initials: AK
authors.lastname: Pereira
authors.forename: H V
authors.initials: HV
authors.lastname: Leite
authors.forename: A P
authors.initials: AP
authors.lastname: Brum
authors.forename: V C
authors.initials: VC
authors.lastname: Pardini
authors.forename: K B
authors.initials: KB
authors.lastname: Gomes
authors.forename: A C V
authors.initials: AC
authors.lastname: Cabral
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology / Q000378:metabolism*; D001483:Base Sequence; D002454:Cell Differentiation; D015925:Cryopreservation; D017931:DNA Primers; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D000071317:Nanog Homeobox Protein; D011247:Pregnancy; D055748:SOXB1 Transcription Factors / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors