title: MMP-2 gene polymorphisms in type 2 diabetes mellitus diabetic retinopathy.
abstract: OBJECTIVE
  To study the association between polymorphisms of the MMP-2 gene and diabetic retinopathy (DR).
  
  
  METHODS
  MMP-2 C-1306T and C-735T SNPs was genotyped by polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) analysis in 151 DR patients and 150 healthy individuals served as control.
  
  
  RESULTS
  There is no significant difference between the patient and control groups in allele or genotype distributions of MMP-2 C-735T (P=0.263 and P=0.248). Also, there is no significant difference between the patient and control in allele of MMP-2 C-1306T (P=0.03). However the result has significant deviation of C/C, C/T, T/T genotypic frequencies between the patient and control groups in MMP-2 C-1306T (P=0.008). We found that subjects with the MMP-2 C-1306T genotype had an overall 2-fold increase in the risk of developing DR [adjusted odds ratio (OR)=2.446; 95% confidence interval (CI)=1.239-4.829] compared with those with the T-1306T or C-1306T genotype. Stratification analysis showed that the MMP-2 -1306C/T and -735C/T SNPs are not associated with the development of NPDR to PDR of DR in North Chinese Han population.
  
  
  CONCLUSIONS
  MMP-2 C-1306T genotypes may be associated with DR development in the Chinese population. However, there is no relationship between the MMP-2 C-735T genotypes with the development of DR.
authors.affiliation: Department of Ophthalmology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Fan
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Guan
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Xin-Zhi
authors.initials: XZ
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Liu