title: Nuclear distributions of NUP62 and NUP214 suggest architectural diversity and spatial patterning among nuclear pore complexes.
abstract: The shape of nuclei in many adherent cultured cells approximates an oblate ellipsoid, with contralateral flattened surfaces facing the culture plate or the medium. Observations of cultured cell nuclei from orthogonal perspectives revealed that nucleoporin p62 (NUP62) and nucleoporin 214 (NUP214) are differentially distributed between nuclear pore complexes on the flattened surfaces and peripheral rim of the nucleus. High resolution stimulated emission depletion (STED) immunofluorescence microscopy resolved individual NPCs, and suggested both heterogeneity and microheterogeneity in NUP62 and NUP214 immunolabeling among in NPC populations. Similar to nuclear domains and interphase chromosome territories, architectural diversity and spatial patterning of NPCs may be an intrinsic property of the nucleus that is linked to the functions and organization of underlying chromatin.
authors.affiliation: Department of Pathology, Mount Sinai School of Medicine, New York, New York, USA.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: Tamara
authors.initials: T
authors.lastname: Kalir
authors.forename: Peter
authors.initials: P
authors.lastname: Dottino
authors.forename: D Stave
authors.initials: DS
authors.lastname: Kohtz
mesh_terms: D002451:Cell Compartmentation; D002452:Cell Count; D045744:Cell Line, Tumor; D053144:Cell Nucleus Shape; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000378:metabolism; D013194:Staining and Labeling; D014162:Transfection
chemical_list: D008562:Membrane Glycoproteins; C085914:NUP214 protein, human; D028861:Nuclear Pore Complex Proteins; D034741:RNA, Small Interfering; C049312:nuclear pore protein p62