title: Phosphoproteomic analysis of electroacupuncture analgesia in an inflammatory             pain rat model.
abstract: The phosphorylation changes of nociceptive signaling proteins in the spinal             cord dorsal horn (SCDH) are important in creating exaggerated pain following peripheral             inflammation. Electroacupuncture (EA) has been widely used to relieve acute and             chronic inflammatory pain in human and experimental pain models. In the present             study, we performed a phosphoproteomic analysis to investigate whether EA alters             protein phosphorylation in SCDH to attenuate pain development. Inflammatory hyperalgesia             was induced by intraplantar injection of complete Freund's adjuvant (CFA) into             the rat hind paw. EA treatment at ST36 and SP6 acupoints alleviated thermal hyperalgesia             of the CFA-induced inflammatory pain model rats. The SCDH proteins from the control,             inflammatory pain model and EA treatment rats were separated by 2-dimensional             gel electrophoresis and the alterations in phosphoproteins were detected by Pro-Q             Diamond staining. Eight proteins were differentially phosphorylated following             EA treatment in the inflammatory pain model. Aldolase C, nascent polypeptide-associated             complex α, stress-induced phosphoprotein 1 and heat shock protein 90 were identified             as phosphoproteins whose expression was increased, whereas GDP dissociation inhibitor             1, thiamine triphosphatase, phosphoglycerate kinase 1 and 14-3-3 γ were phosphoproteins             whose expression was decreased. This is the first phosphoproteomic screening study             to elucidate the working mechanisms of EA analgesia. The results suggest that             the regulation of cellular pathways in which the identified proteins are involved             may be associated with an EA analgesic mechanism.
authors.affiliation: Division of Meridian and Structural Medicine, School of Korean                     Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
authors.forename: Si-Hyoung
authors.initials: SH
authors.lastname: Lee
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Ji-Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Hye-Yun
authors.initials: HY
authors.lastname: Jung
authors.forename: Jeong-Hee
authors.initials: JH
authors.lastname: Moon
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
authors.forename: Byung-Tae
authors.initials: BT
authors.lastname: Choi
mesh_terms: D000698:Analgesia; D000818:Animals; D004195:Disease Models, Animal; D015671:Electroacupuncture; D005620:Freund's Adjuvant / Q000009:adverse effects; D007249:Inflammation / Q000139:chemically induced / Q000628:therapy; D008297:Male; D010146:Pain / Q000139:chemically induced / Q000378:metabolism*; D059408:Pain Management; D010750:Phosphoproteins / Q000378:metabolism*; D020671:Posterior Horn Cells / Q000378:metabolism; D020543:Proteome; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010750:Phosphoproteins; D020543:Proteome; D005620:Freund's Adjuvant