title: A comprehensive proteome of Mycoplasma genitalium.
abstract: Mycoplasma genitalium is a human pathogen associated with several sexually transmitted diseases. Proteomic technologies, along with other methods for global gene expression analysis, play a key role in understanding the mechanisms of bacterial pathogenesis and physiology. The proteome of M. genitalium, model of a minimal cell, has been extended using a combination of different proteomic approaches and technologies. The total proteome of this microorganism has been analyzed using gel-based and gel-free approaches, achieving the identification of 85.3% of the predicted ORFs. In addition, a comprehensive analysis of membrane subproteome has been performed. For this purpose, the TX-114 soluble fraction has been analyzed as well as the surface proteins, using cell-surface protein labeling with CyDye. Finally, the serological response of M. genitalium-infected patients and healthy donors has been analyzed to identify proteins that trigger immunological response. Here, we present the most extensive M. genitalium proteome analysis (85.3% of predicted ORFs), a comprehensive M. genitalium membrane analysis, and a study of the human serological response to M. genitalium.
authors.affiliation: Institut de Biotecnologia i de Biomedicina (IBB) and Dpt Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona (UAB), E-08193 Cerdanyola del Vallès (Barcelona), Spain.
authors.forename: Noemí
authors.initials: N
authors.lastname: Párraga-Niño
authors.forename: Nuria
authors.initials: N
authors.lastname: Colomé-Calls
authors.forename: Francesc
authors.initials: F
authors.lastname: Canals
authors.forename: Enrique
authors.initials: E
authors.lastname: Querol
authors.forename: Mario
authors.initials: M
authors.lastname: Ferrer-Navarro
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D016022:Case-Control Studies; D006801:Humans; D009175:Mycoplasma Infections / Q000097:blood / Q000276:immunology / Q000382:microbiology; D045704:Mycoplasma genitalium / Q000276:immunology / Q000378:metabolism*; D020543:Proteome / Q000276:immunology / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D020543:Proteome