title: On-target labeling of intracellular metabolites combined with chemical mapping of individual hyphae revealing cytoplasmic relocation of isotopologues.
abstract: A microscale analytical platform integrating microbial cell culture, isotopic labeling, along with visual and mass spectrometric imaging with single-cell resolution has been developed and applied in the monitoring of cellular metabolism in fungal mycelium. The method implements open chips with a two-dimensional surface pattern composed of hydrophobic and hydrophilic zones. Two hydrophilic islands are used as medium reservoirs, while the hydrophobic area constitutes the support for the growing aerial hyphae, which do not have direct contact with the medium. The first island, containing (12)C(6)-glucose medium, was initially inoculated with the mycelium (Neurospora crassa), and following the initial incubation period, the hyphae progressed toward the second medium island, containing an isotopically labeled substrate ((13)C(6)-glucose). The (13)C atoms were gradually incorporated into cellular metabolites, which was revealed by MALDI-MS. The fate of the chitin-biosynthesis precursor, uridine diphosphate N-acetylglucosamine (UDP-GlcNAc), was monitored by recording mass spectra with characteristic isotopic patterns, which indicated the presence of various (12)C/(13)C isotopologues. The method enabled mapping the (13)C-labeled UDP-GlcNAc in fungal mycelium and recording its redistribution in hyphae, directly on the chip.
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd, Hsinchu, 300, Taiwan.
authors.forename: Jie-Bi
authors.initials: JB
authors.lastname: Hu
authors.forename: Yu-Chie
authors.initials: YC
authors.lastname: Chen
authors.forename: Pawel L
authors.initials: PL
authors.lastname: Urban
mesh_terms: D002686:Chitin / Q000032:analysis* / Q000096:biosynthesis; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D025301:Hyphae / Q000737:chemistry* / Q000378:metabolism; D007553:Isotope Labeling; D056656:Lab-On-A-Chip Devices; D055432:Metabolomics; D009492:Neurospora crassa / Q000737:chemistry* / Q000378:metabolism; D059010:Single-Cell Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000032:analysis* / Q000378:metabolism
chemical_list: D003470:Culture Media; D002686:Chitin; D014537:Uridine Diphosphate N-Acetylglucosamine; D005947:Glucose