title: Two new species of the genus Ochroconis, O. lascauxensis and O. anomala isolated from black stains in Lascaux Cave, France.
abstract: In the year 2001, some conspicuous black stains appeared on the walls of Lascaux Cave in France, which progressively disseminated throughout the cave. These black stains were so evident by 2007 that they have become one of the cave's major problems. In a mycological study of the black stains, Ochroconis strains were abundant among the isolates and constituted the major group of melanised fungi. Two new species of the genus Ochroconis, O. lascauxensis and O. anomala, were isolated and described. The description is based on the morphology of the fungi and the phylogenetic relationships of two of its gene regions internal transcribed spacer (ITS) and RNA polymerase II subunit B (RPB2). In addition, data on their physiology and cellular fatty acid profiles are reported. The development of these species was likely linked to the presence of unusual carbon and nitrogen organic sources provided by the intensive biocide treatments.
authors.affiliation: Instituto de Recursos Naturales y Agrobiología de Sevilla, Consejo Superior de Investigaciones Científicas, Av. Reina Mercedes, 10, 41012 Sevilla, Spain.
authors.forename: Pedro Maria
authors.initials: PM
authors.lastname: Martin-Sanchez
authors.forename: Alena
authors.initials: A
authors.lastname: Nováková
authors.forename: Fabiola
authors.initials: F
authors.lastname: Bastian
authors.forename: Claude
authors.initials: C
authors.lastname: Alabouvette
authors.forename: Cesareo
authors.initials: C
authors.lastname: Saiz-Jimenez
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D059552:Caves / Q000382:microbiology*; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005602:France; D008969:Molecular Sequence Data; D010151:Paintings; D010802:Phylogeny
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer