title: MiR-21 is enriched in the RNA-induced silencing complex and targets COL4A1 in human granulosa cell lines.
abstract: MicroRNAs (miRNAs) are noncoding small RNAs that play important roles in a variety of physiological and pathological events. In this study, we performed large-scale profiling of EIF2C2-bound miRNAs in 3 human granulosa-derived cell lines (ie, KGN, HSOGT, and GC1a) by high-throughput sequencing and found that miR-21 accounted for more than 80% of EIF2C2-bound miRNAs, suggesting that it was enriched in the RNA-induced silencing complex (RISC) and played a functional role in human granulosa cell (GC) lines. We also found high expression levels of miR-21 in primary human GCs. Assuming that miR-21 target mRNAs are enriched in RISC, we performed cDNA cloning of EIF2C2-bound mRNAs in KGN cells. We identified COL4A1 mRNA as a miR-21 target in the GC lines. These data suggest that miR-21 is involved in the regulation of the synthesis of COL4A1, a component of the basement membrane surrounding the GC layer and granulosa-embedded extracellular structure.
authors.affiliation: Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Mase
authors.forename: Osamu
authors.initials: O
authors.lastname: Ishibashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Takami
authors.initials: T
authors.lastname: Takizawa
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kiguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohba
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Katabuchi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takeshita
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Takizawa
mesh_terms: D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism; D002268:Carboxypeptidases / Q000378:metabolism*; D002460:Cell Line; D024141:Collagen Type IV / Q000096:biosynthesis*; D005260:Female; D020869:Gene Expression Profiling; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; C547475:COL4A1 protein, human; D024141:Collagen Type IV; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D002268:Carboxypeptidases; C436327:SCPEP1 protein, human