title: Seasons can influence the results of the methacholine challenge test.
abstract: OBJECTIVE
  This study tried to evaluate whether a methacholine test may be influenced by the seasons.
  
  
  METHODS
  We considered 4826 consecutive subjects with normal spirometry (50.53% males; age: 35.1±16.2; forced expiratory volume in one second: 99.5±13.0%) who underwent a methacholine test for suspected asthma symptoms between 2000 and 2010. They were subdivided into four groups, like the seasons, according to the test dates.
  
  
  RESULTS
  A total of 1981 (41%) resulted normal (no PD(20) was obtained with 2400 μg of methacholine); the others showed a mean LogPD(20) of 2.52±0.5 μg. The number of subjects with bronchial hyper-responsiveness (BHR) found in autumn (789, 62.3%) was higher than in summer (583, 56.7%; P=0.03). A higher number of females and overweight/obese subjects showed a BHR in autumn compared with the other seasons. The spring mean LogPD(20) value (2.48±0.48 μg) was lower if compared with the one measured in summer (2.59±0.49 μg; P=0.05). LogPD(20) value was lower in females and non-smokers in spring compared with summer (P<0.05). Overweight/obese non-smokers showed a lower LogPD(20) in spring and autumn compared with that in summer (P<0.05). Autumn was a risk factor (OR: 1.378; P=0.001) for BHR (using a PD(20) <2 400 μg as BHR limit), while spring (OR: 1.330; P=0.021) and autumn (OR: 1.331; P=0.020) were risk factors for a more severe BHR (using a PD(20) <400 μg as BHR limit).
  
  
  CONCLUSIONS
  There was a higher probability of finding BHR in outpatients with suspected asthma in autumn and spring compared with summer. Spring is the season where BHR may be more severe. Females and overweight/obese subjects were those mainly involved in this seasonal variability of BHR.
authors.affiliation: Unit of Pneumology, "Misericordia" Hospital, Grosseto, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Sposato
authors.forename: Marco
authors.initials: M
authors.lastname: Scalese
authors.forename: Andrea
authors.initials: A
authors.lastname: Pammolli
authors.forename: Raffaele
authors.initials: R
authors.lastname: Scala
authors.forename: Mario
authors.initials: M
authors.lastname: Naldi