title: Effects of various salinities on Na(+)-K(+)-ATPase, Hsp70 and Hsp90 expression profiles in juvenile mitten crabs, Eriocheir sinensis.
abstract: Eriocheir  sinensis is a euryhaline crab migrating from sea to freshwater  habitats during the juvenile stage. We used quantitative real-time polymerase  chain reaction (qRT-PCR) to investigate the gene expression profile of Na(+)-K(+)-ATPase, Hsp70 (heat shock protein 70) and Hsp90 in megalopa exposed to salinities  of 0, 2, 5, 10, and 15 parts per thousand. Both low and high salinities  markedly stimulated expression of Na(+)-K(+)-ATPase, Hsp70 and Hsp90 genes of Chinese mitten crab megalopa; salinity had  different effects on Na(+)-K(+)-ATPase, Hsp70 and Hsp90 levels depending on the duration of salinity stress,  implying that Na(+)-K(+)-ATPase, Hsp70 and Hsp90 may play an important role in salinity tolerance in this  crab species.
authors.affiliation: Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: M
authors.initials: M
authors.lastname: Sun
authors.forename: K
authors.initials: K
authors.lastname: Jiang
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Zhang
authors.forename: A
authors.initials: A
authors.lastname: Shen
authors.forename: M
authors.initials: M
authors.lastname: Jiang
authors.forename: X
authors.initials: X
authors.lastname: Shen
authors.forename: L
authors.initials: L
authors.lastname: Ma
mesh_terms: D000818:Animals; D001483:Base Sequence; D003445:Crustacea / Q000235:genetics*; D017931:DNA Primers; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012965:Sodium Chloride / Q000032:analysis*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D014867:Water / Q000737:chemistry*
chemical_list: D017931:DNA Primers; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D014867:Water; D012965:Sodium Chloride; D000254:Sodium-Potassium-Exchanging ATPase