title: Synthesis of nucleoside phosphosulfates.
abstract: We describe an efficient and scalable procedure for the chemical synthesis of nucleoside 5'-phosphosulfates (NPS) from nucleoside 5'-phosphorimidazolides and sulfate bis(tributylammonium) salt. Using this method we obtained various NPS with yields ranging from 70-90%, including adenosine 5'-phosphosulfate (APS) and 2',3'-cyclic precursor of 3'-phosphoadenosine 5'-phosphosulfate (PAPS), which are the key intermediates in the assimilation and metabolism of sulfur in all living organisms.
authors.affiliation: Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kowalska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Osowniak
authors.forename: Joanna
authors.initials: J
authors.lastname: Zuberek
authors.forename: Jacek
authors.initials: J
authors.lastname: Jemielity
mesh_terms: D000250:Adenosine Phosphosulfate / Q000138:chemical synthesis / Q000737:chemistry*; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism; D009705:Nucleosides / Q000737:chemistry*; D010724:Phosphoadenosine Phosphosulfate / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D009705:Nucleosides; D010724:Phosphoadenosine Phosphosulfate; D000250:Adenosine Phosphosulfate