title: Alpha-mannosidase activity in stallion epididymal fluid and spermatozoa.
abstract: The expression of α-D-mannosidase activity was fluorometrically and electrophoretically assessed in spermatozoa, epididymal fluid and homogenates of stallion epididymal tissue. Enzyme activity had regional differences; it was higher (P<0.05) in samples from the cauda epididymal region than in samples from the proximal caput region (largely composed of efferent ducts). Based on enzyme activity, as a function of pH of the assay substrate, electrophoretic analysis in native and native/SDS-PAGE conditions, and the effect of inhibitors or activators, we inferred the presence of at least two catalytically active forms of α-D-mannosidase. The neutral form of the enzyme (α-mannosidase II) was activated by Co2+, whereas the acid form (optimum pH 3.5 to 4.0) was sensitive to swainsonine (an inhibitor of α-mannosidase I), stabilized or stimulated by Zn2+, and not activated by Co2+ (activator of the neutral form). The activity of the acid form of the enzyme was highest in the epididymal fluid, where it seemed to be mainly in a secretory form. This form of the enzyme may have a role in plasma membrane remodeling associated with sperm maturation. In contrast, the activity of α-mannosidase II was higher in mature spermatozoa. It has been postulated that α-mannosidase II may act as a receptor in the recognition and binding of the complementary carbohydrate moieties present on the zona pellucida. With non-denaturing electrophoresis, α-D-mannosidase had an electrophoretic mobility of 0.35 and 0.24. When resolved by 1D and 2D SDS-PAGE (under denaturing conditions) the enzyme had a major protein band of molecular weight 154 kDa in spermatozoa and epididymal samples. Based on its properties under native conditions, we inferred that this enzyme might interact with other proteins and form transitory aggregates.
authors.affiliation: Setor Biologia da Reprodução, Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense and Av. Alberto Lamego 2000 Campos dos Goytacazes, RJ, Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: Retamal
authors.forename: A J B
authors.initials: AJ
authors.lastname: Dias
authors.forename: F C
authors.initials: FC
authors.lastname: Brasil
authors.forename: F R
authors.initials: FR
authors.lastname: Lanzana
authors.forename: M L
authors.initials: ML
authors.lastname: López
mesh_terms: D000818:Animals; D002712:Chlorides / Q000494:pharmacology; D003035:Cobalt / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004822:Epididymis / Q000201:enzymology / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006736:Horses / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D012661:Semen / Q000201:enzymology*; D013094:Spermatozoa / Q000201:enzymology*; D017026:Swainsonine / Q000494:pharmacology; D017967:Zinc Compounds / Q000494:pharmacology; D043323:alpha-Mannosidase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D002712:Chlorides; D004791:Enzyme Inhibitors; D017967:Zinc Compounds; D003035:Cobalt; C016837:zinc chloride; D043323:alpha-Mannosidase; C018021:cobaltous chloride; D017026:Swainsonine