title: A rhodamine chromene-based turn-on fluorescence probe for selectively imaging Cu²+ in living cell.
abstract: We describe the development of a rhodamine chromene-based turn-on fluorescence probe to monitor the intracellular Cu(2+) level in living cells. The new fluorescent probe with a chlorine group in chromene moiety exhibits good membrane-permeable property than previous reported because the predicted lipophilicity of present probe 4 is stronger than that of methoxyl substituted probe in our previous work (CLogP of 4: 8.313, CLogP of methoxyl substituted probe: 7.706), and a fluorescence response toward Cu(2+) under physiological conditions with high sensitivity and selectivity, and facilitates naked-eye detection of Cu(2+). The fluorescence intensity was remarkably increased upon the addition of Cu(2+) within 1 or 2 min, while the other sixteen metal ions caused no significant effect.
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Wei-Yong
authors.initials: WY
authors.lastname: Liu
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Li
authors.forename: Hong-Shui
authors.initials: HS
authors.lastname: Lv
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
mesh_terms: D000042:Absorption; D001578:Benzopyrans / Q000138:chemical synthesis / Q000737:chemistry*; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival; D005110:Extracellular Space / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D057054:Molecular Imaging / Q000379:methods*; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D001578:Benzopyrans; D005456:Fluorescent Dyes; D007477:Ions; D012235:Rhodamines; D002118:Calcium