title: A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells.
abstract: A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Tahara
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kaneko
authors.forename: Riki
authors.initials: R
authors.lastname: Toita
authors.forename: Chiharu
authors.initials: C
authors.lastname: Yoshiyama
authors.forename: Takuya
authors.initials: T
authors.lastname: Kitaoka
authors.forename: Takuro
authors.initials: T
authors.lastname: Niidome
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Katayama
authors.forename: Noriho
authors.initials: N
authors.lastname: Kamiya
authors.forename: Masahiro
authors.initials: M
authors.lastname: Goto
mesh_terms: D000818:Animals; D016466:CHO Cells; D002784:Cholesterol / Q000737:chemistry; D006224:Cricetinae; D003412:Cricetulus; D045304:Cytochromes c / Q000008:administration & dosage*; D004247:DNA / Q000008:administration & dosage; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000008:administration & dosage*; D036103:Nanotechnology / Q000379:methods; D009821:Oils / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry; D010047:Ovalbumin / Q000008:administration & dosage*; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D013228:Stearates / Q000737:chemistry; D014162:Transfection / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D004337:Drug Carriers; D009821:Oils; D009842:Oligopeptides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013228:Stearates; C487882:stearyl-octaarginine; D014867:Water; D010047:Ovalbumin; D045304:Cytochromes c; D004247:DNA; D002784:Cholesterol; D006735:Horseradish Peroxidase