title: Unraveling the rationale behind organic solvent stability of lipases.
abstract: Organic solvent-stable lipases have pronounced impact on industrial economy as they are involved in synthesis by esterification, interesterification, and transesterification. However, very few of such natural lipases have been isolated till date. A study of the recent past provided few pillars to rely on for this work. The three-dimensional structure, inclusive of the surface and active site, of 29 organic solvent-stable lipases was analyzed by subfamily classification and protein solvent molecular docking based on fast Fourier transform correlation approach. The observations revealed that organic solvent stability of lipases is their intrinsic property and unique with respect to each lipase. In this paper, factors like surface distribution of charged, hydrophobic, and neutral residues, interaction of solvents with catalytically immutable residues, and residues interacting with essential water molecules required for lipase activity, synergistically and by mutualism contribute to render a stable lipase organic solvent. The propensity of surface charge in relation to stability in organic solvents by establishing repulsive forces to exclude solvent molecules from interacting with the surface and prohibiting the same from gaining entry to the protein core, thus stabilizing the active conformation, is a new finding. It was also interesting to note that lipases having equivalent surface-exposed positive and negative residues were stable in a wide range of organic solvents, irrespective of their LogP values.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Guwahati 781039, Assam, India.
authors.forename: Debamitra
authors.initials: D
authors.lastname: Chakravorty
authors.forename: Saravanan
authors.initials: S
authors.lastname: Parameswaran
authors.forename: Vikash Kumar
authors.initials: VK
authors.lastname: Dubey
authors.forename: Sanjukta
authors.initials: S
authors.lastname: Patra
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology; D020134:Catalytic Domain; D019295:Computational Biology; D004795:Enzyme Stability; D005658:Fungi / Q000201:enzymology; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D009930:Organic Chemicals / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
chemical_list: D009930:Organic Chemicals; D012997:Solvents; D008049:Lipase