title: Acidic preconditioning protects against ischemia-induced brain injury.
abstract: Ischemic preconditioning protects against cerebral ischemia. Recent investigations indicated that acidic preconditioning (APC) protects against ischemia-induced cardiomyocytes injury. However, it is not clear whether APC can protect against cerebral ischemia. To address this issue, C57BL/6 mice were exposed 3 times at 10-min intervals to a normoxic atmosphere containing 20% CO(2) for 5 min before being further subjected to bilateral common carotid artery occlusion. APC reversed the ischemia-induced brain injury as revealed by improved performance in passive avoidance experiments and decreased neuron loss in the hippocampal CA1 region. Consistently, both APC-treated brain slices and primary cultured neurons were more resistant to oxygen-glucose-deprivation (OGD)-induced injury, in a pH- and time-dependent manner, as revealed by reversed cell/tissue viability. In addition, the APC treatment prevented OGD-induced mitochondrial transmembrane potential loss and apoptosis, which was inhibited by the mitochondrial permeability transport pore opener atractyloside. Taken together, these findings indicated that APC protects against ischemia-induced neuronal injury. The beneficial effects may be attributed, at least in part, to decreased mitochondria-dependent neuronal apoptosis.
authors.affiliation: Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical College, Wenzhou 325035, China.
authors.forename: Chen-hui
authors.initials: CH
authors.lastname: Zhang
authors.forename: Yan-ying
authors.initials: YY
authors.lastname: Fan
authors.forename: Xiao-fen
authors.initials: XF
authors.lastname: Wang
authors.forename: Jia-yan
authors.initials: JY
authors.lastname: Xiong
authors.forename: Ying-ying
authors.initials: YY
authors.lastname: Tang
authors.forename: Jie-qiong
authors.initials: JQ
authors.lastname: Gao
authors.forename: Zhe
authors.initials: Z
authors.lastname: Shen
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Song
authors.forename: Jing-ying
authors.initials: JY
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Shen
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Xiangnan
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000138:Acidosis; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D001930:Brain Injuries / Q000175:diagnosis / Q000503:physiopathology* / Q000628:therapy*; D002545:Brain Ischemia / Q000175:diagnosis / Q000503:physiopathology* / Q000628:therapy*; D002245:Carbon Dioxide / Q000008:administration & dosage*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016896:Treatment Outcome
chemical_list: D002245:Carbon Dioxide