title: Dynamics of endothelial progenitor cells following sevoflurane preconditioning.
abstract: There is relevant evidence concerning the involvement of endothelial progenitor cells in neovascularization and wound healing. In this study we investigated the effects of sevoflurane, a volatile anesthetic with proven cardioprotective virtues, on the mobilization of bone marrow mononuclear cells with endothelial progenitor markers (CD 34+, flk-1 +), an event that may account for the protective effects of delayed anesthetic preconditioning. Male Wistar rats were treated with a mixture of air and sevoflurane (1 MAC) in cycles of 5 minutes, alternating with 5-minutes wash-out periods (the preconditioned group), or ventilated for 30 minutes with room air (control group). Following flow cytometry and immunofluorescence measurements, a considerable increase in circulating CD34+, flk-1 + and CD34+/flk-1 + cells was observed in the preconditioned group beginning at 12 hours after treatment, with a peak value at 24 hours after sevoflurane administration. These cells are a potential source of myocardial regeneration in the context of perioperative or periprocedural ischemia in patients with coronary artery disease.
authors.affiliation: Physiology Department, "Carol Davila" UMPh, Bucharest, Romania.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Popescu
authors.forename: Adelina
authors.initials: A
authors.lastname: Munteanu
authors.forename: Gheorghiţa
authors.initials: G
authors.lastname: Isvoranu
authors.forename: Laura
authors.initials: L
authors.lastname: Suciu
authors.forename: Bogdan
authors.initials: B
authors.lastname: Pavel
authors.forename: Bogdan
authors.initials: B
authors.lastname: Marinescu
authors.forename: Leon
authors.initials: L
authors.lastname: Zagrean
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D042783:Endothelial Cells / Q000187:drug effects*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000077149:Sevoflurane; D013234:Stem Cells / Q000187:drug effects*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000032:analysis
chemical_list: D018685:Anesthetics, Inhalation; D018952:Antigens, CD34; D008738:Methyl Ethers; D000077149:Sevoflurane; D040301:Vascular Endothelial Growth Factor Receptor-2