title: Neurotrophin expression in neural stem cells grafted acutely to transected spinal cord of adult rats linked to functional improvement.
abstract: It is well known that neural stem cells (NSC) could promote the repairment after spinal cord injury, but the underlying mechanism remains to be elucidated. This study showed that the transplantation of NSC significantly improved hindlimb locomotor functions in adult rats subjected to transection of the spinal cord. Biotin dextran amine tracing together with the stimulus experiment in motor sensory area showed that little CST regeneration existed and functional synaptic formation in the injury site. Immunocytochemistry and RT-PCR demonstrated the secretion of NGF, BDNF, and NT-3 by NSC in vitro and in vivo, respectively. However, only mRNA expression of BDNF and NT-3 but not NGF in injury segment following NSC transplantation was upregulated remarkably, while caspase-3, a crucial apoptosis gene, was downregulated simultaneously. These provided us a clue that the functional recovery was correlated with the regulation of BDNF, NT-3, and caspase-3 in spinal cord transected rats following NSC transplantation.
authors.affiliation: Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Ying-Li
authors.initials: YL
authors.lastname: Gu
authors.forename: Lu-Wei
authors.initials: LW
authors.lastname: Yin
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Su-Juan
authors.initials: SJ
authors.lastname: Liu
authors.forename: Lian-Feng
authors.initials: LF
authors.lastname: Zhang
authors.forename: Ting-Hua
authors.initials: TH
authors.lastname: Wang
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis*; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006085:Graft Survival / Q000502:physiology; D009414:Nerve Growth Factors / Q000096:biosynthesis; D009416:Nerve Regeneration / Q000502:physiology; D058953:Neural Stem Cells / Q000637:transplantation*; D020933:Neurotrophin 3 / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000601:surgery; D033581:Stem Cell Transplantation / Q000379:methods; D015854:Up-Regulation / Q000502:physiology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D020933:Neurotrophin 3