title: Antibacterial effect of a resin incorporating a novel polymerizable quaternary ammonium salt MAE-DB against Streptococcus mutans.
abstract: The antibacterial properties of resins incorporating MAE-DB and the underlying mechanisms of action were evaluated. Antibacterial effects against Streptococcus mutans were tested using the film contact method, with accumulation and membrane integrity observed by scanning electron microscopy and confocal laser scanning microscopy, respectively. Quantitative PCR was used to determine expression of the S. mutans glucosyltransferase B (gtfB) gene on the surface of resins containing 10% MAE-DB. Bacterial growth was inhibited on resin containing 10% MAE-DB as compared with the control at 1 day, 7 days, 30 days, or 180 days (p < 0.05). For the 10%-MAE-DB resin, no significant differences in bacterial viability were found regardless of the time of incubation (p > 0.05). The number of bacteria attached to resin containing 10% MAE-DB was considerably lower than the control. The proportion of bacteria with damaged cell membranes was increased in the experimental resin over controls. Expression of gtfB was reduced by 10% MAE-DB compared with the control (p < 0.05). These findings demonstrate that MAE-DB can be incorporated into resin materials at sufficient concentrations for long-term antibacterial effects against S. mutans after polymerization by attenuating gtfB expression and impairing membrane integrity.
authors.affiliation: Department of General Dentistry and Emergency, School of Stomatology, Fourth Military Medical University, Xi'an 710032, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Xiang
authors.initials: X
authors.lastname: Sun
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Xiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Zhong-Chun
authors.initials: ZC
authors.lastname: Tong
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Xing
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Chai
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Chen
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005964:Glucosyltransferases / Q000096:biosynthesis; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000494:pharmacology*; D012117:Resins, Synthetic / Q000737:chemistry / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000254:growth & development*; D013997:Time Factors
chemical_list: C560118:2-methacryloxylethyl dodecyl methyl ammonium bromide; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008689:Methacrylates; D000644:Quaternary Ammonium Compounds; D012117:Resins, Synthetic; D005964:Glucosyltransferases