title: Effect of pan-retinal laser photocoagulation on plasma VEGF, endothelin-1 and nitric oxide in PDR.
abstract: OBJECTIVE
  To study plasma levels of vascular endothelial growth factor (VEGF), endothelin-1(ET-1) and nitric oxide (NO) in patients with proliferative diabetic retinopathy (PDR) before and after pan-retinal photocoagulation (PRP).
  
  
  METHODS
  Forty patients (23 females and 17 males, mean age 48.5±12.2 years) with PDR without previous PRP therapy were studied. Blood samples were obtained before and 3 months after the last PRP session. Baseline (prelaser) plasma levels of VEGF, ET-1 and NO were compared with their levels in 30 healthy age- and sex- matched controls and also with plasma levels 3 months post-PRP.
  
  
  RESULTS
  Patients with PDR had significantly raised plasma VEGF (375±89ng/L), ET-1(20±5ng/L) and NO (135±53µmol/L) when compared with healthy control group (P<0.01). After PRP, there was a significant reduction in plasma VEGF (179±66ng/L), ET-1 (11±5ng/L) and NO (91±49µmol/L) levels at 3 months' follow-up but still significantly higher than healthy controls.
  
  
  CONCLUSIONS
  Patients with PDR demonstrate elevated VEGF, ET-1 and NO, which decrease after successful laser treatment.
authors.affiliation: Department of Ophthalmology, Assiut University Hospitals, Assiut University, Assiut, Egypt.
authors.forename: Tarek Ahmed
authors.initials: TA
authors.lastname: Mohamed
authors.forename: Sahar El-Deek
authors.initials: Sel-D
authors.lastname: Mohamed