title: Cryopreservation of winter-dormant apple: iii - bud water status and survival after cooling to -30°C and during recovery from cryopreservation.
abstract: In a continuing study to improve the efficiency of dormant bud cryopreservation for tissues hardened in maritime climates, the water status of dormant buds was monitored between -4 degree C and recovery from liquid nitrogen (LN). Measurement of water content, simple thermal analysis and differential scanning calorimetry were employed. Buds did not lose water during cooling to, or holding at -30 degree C indicating that cryodehydration and/or other adaptive responses contributed during this essential step. A bud exotherm that was an artefact of warming was detected due to necessary handling at -4 degree C before cooling to -30 degree C. There were no significant differences between cultivars with respect to water status at -30 degree C or immediately upon rewarming from LN despite significant differences in post-LN survival. Buds rehydrated in 5 days, but up to 14 days may be needed for recovery for some cultivars. In some instances buds could be grafted without rehydration, taking up water across the early graft union.
authors.affiliation: Department of Agriculture and Ecology, University of Copenhagen, Taastrup, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Vogiatzi
authors.forename: B W W
authors.initials: BW
authors.lastname: Grout
authors.forename: A
authors.initials: A
authors.lastname: Wetten
mesh_terms: D002152:Calorimetry, Differential Scanning; D015925:Cryopreservation; D027845:Malus / Q000502:physiology*; D018520:Plant Shoots / Q000502:physiology; D012621:Seasons; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water