title: Copolymer nanoparticles composed of sulfobetaine and poly(ε-caprolactone) as novel anticancer drug carriers.
abstract: Novel ABA type amphiphilic copolymers (PCL-APS-PCL) consisting of polycaprolactone (PCL) (A) as hydrophobic block and N,N'-bis (2-hydroxyethyl) methylamine ammonium propane sulfonate (APS) (B) as hydrophilic segment, self-assembled into nanoparticles (NPs) with solvent evaporation method. The sizes and size distributions of NPs were characterized by dynamic light scattering. The morphology of NPs was observed by scanning electron microscopy (SEM). The critical micelle concentration (CMC) was determined by fluorescent probe. The drug loading content (DLC) and the drug release amount were characterized by UV-visible spectrophotometer. The cytotoxicity of the NPs was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazoliumbromide (MTT) assay. It was found that the NPs were spherical in shape with sizes around 100 nm. The CMCs of the copolymers were quite low (×10(-4) mg/mL). The DLC decreased with lengthening of hydrophobic PCL block. In vitro drug release experiment demonstrated that the release rate of paclitaxel sped with the decrease of PCL length. MTT results showed that NPs were nontoxic to osteoblast and human epithelial carcinoma (hela) cells. After drug loading, NPs could restrain the growth of hela or even kill hela cells. Therefore, these preliminary studies suggest that the novel PCL-APS-PCL NPs have a great potential application as anticancer drug-delivery carriers.
authors.affiliation: College of Polymer Science and Engineering of Sichuan University, Sichuan University, Sichuan 610065, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Ke-Mao
authors.initials: KM
authors.lastname: Xiu
authors.forename: Kun
authors.initials: K
authors.lastname: Zhu
authors.forename: Yuan-Wei
authors.initials: YW
authors.lastname: Chen
authors.forename: Xiang-Lin
authors.initials: XL
authors.lastname: Luo
mesh_terms: D017738:Alkanesulfonic Acids / Q000737:chemistry; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001622:Betaine / Q000031:analogs & derivatives* / Q000737:chemistry; D016923:Cell Death / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D017239:Paclitaxel / Q000494:pharmacology; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D017738:Alkanesulfonic Acids; D000970:Antineoplastic Agents; D004337:Drug Carriers; D008823:Micelles; D011091:Polyesters; C016240:polycaprolactone; C068559:propylsulfonic acid; D001622:Betaine; C483727:sulfobetaine; D017239:Paclitaxel