title: Phenolic compounds isolated from Psoralea corylifolia inhibit IL-6-induced STAT3 activation.
abstract: Inhibiting interleukin-6 (IL-6) has been postulated as an effective therapy in the pathogenesis of several inflammatory diseases. In this study, seven flavonoids were isolated from the methanol extracts of Psoralea corylifolia by bioactivity-guided fractionation. The structures of bakuchiol (1), bavachinin (2), neobavaisoflavone (3), corylifol A (4), corylin (5), isobavachalcon (6), and bavachin (7) were determined by spectroscopic analysis (1H-, 13C- NMR and MS). We demonstrated that compounds 1-7 showed an inhibitory effect on IL-6-induced STAT3 promoter activity in Hep3B cells with IC50 values of 4.57 ± 0.45, 3.02 ± 0.53, 2.77 ± 0.02, 0.81 ± 0.15, 1.37 ± 0.45, 2.45 ± 0.13, and 4.89 ± 0.05 µΜ, respectively. These compounds also inhibited STAT3 phosphorylation induced by IL-6 in Hep3B cells. Overall, several flavonoids from P. corylifolia might be useful remedies for treating inflammatory diseases by inhibiting IL-6-induced STAT3 activation and phosphorylation.
authors.affiliation: Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Korea.
authors.forename: Seung Woong
authors.initials: SW
authors.lastname: Lee
authors.forename: Bo Ra
authors.initials: BR
authors.lastname: Yun
authors.forename: Mi Hwa
authors.initials: MH
authors.lastname: Kim
authors.forename: Chan Sun
authors.initials: CS
authors.lastname: Park
authors.forename: Woo Song
authors.initials: WS
authors.lastname: Lee
authors.forename: Hyun-Mee
authors.initials: HM
authors.lastname: Oh
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D004353:Drug Evaluation, Preclinical / Q000379:methods; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D015850:Interleukin-6 / Q000494:pharmacology*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D011401:Promoter Regions, Genetic; D029907:Psoralea / Q000737:chemistry*; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014443:Tyrosine / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D047309:Flavones; D005419:Flavonoids; D015850:Interleukin-6; D007529:Isoflavones; D010636:Phenols; D010936:Plant Extracts; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C459212:bavachin; C549829:corylifol A; C459211:corylin; C549830:neobavaisoflavone; D014443:Tyrosine; C012765:bakuchiol; C468752:bavachinin