title: Derivation and characterization of progenitor stem cells from canine allantois and amniotic fluids at the third trimester of gestation.
abstract: Fetal tissues are frequently discarded before (amniocentesis) or after birth, which both facilitates stem cell access and helps to overcome ethical concerns. In the present study, we aimed to isolate and characterize stem cells from the allantoic and amniotic fluids (ALF; AMF) of third trimester canine fetuses. This gestation age has not been previously explored for stem cells isolation. The gestational age, cell culture conditions and method of isolation used in this study allowed for the establishment and efficient expansion of ALF and AMF cells. We showed that the majority of ALF and ALF cells express the stem cell markers, such as vimentin, nestin and cytokeratin 18 (CK18). Under appropriate culture conditions AMF derived cells can undergo differentiation into osteogenic, adipogenic, chondrogenic and neuron-like lineages. ALF derived cells showed adipogenic, and chondrogenic potential. Therefore, ALF and AMF cells derived at the third gestation trimester can be qualified as progenitor stem cells, accordingly referred as (alantoic fluid progenitor/stem) ALF PS cells and (amniotic fluid progenitor/stem) AMF PS cells.
authors.affiliation: University of São Paulo, Faculty of Veterinary Medicine, Department of Surgery and Department of Pathology, São Paulo, Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Fernandes
authors.forename: C V
authors.initials: CV
authors.lastname: Wenceslau
authors.forename: A L
authors.initials: AL
authors.lastname: Reginato
authors.forename: I
authors.initials: I
authors.lastname: Kerkis
authors.forename: M A
authors.initials: MA
authors.lastname: Miglino
mesh_terms: D050156:Adipogenesis; D000482:Allantois / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D000653:Amniotic Fluid / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D003470:Culture Media / Q000378:metabolism; D004285:Dogs; D005260:Female; D005865:Gestational Age; D016130:Immunophenotyping; D007381:Intermediate Filament Proteins / Q000378:metabolism; D053538:Keratin-18 / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D010012:Osteogenesis; D011247:Pregnancy; D057905:Stem Cell Research; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D003470:Culture Media; D007381:Intermediate Filament Proteins; D053538:Keratin-18; D009419:Nerve Tissue Proteins; D064231:Nestin; D014746:Vimentin