title: Characterization of a chitinase (Chit62) from Serratia marcescens B4A and its efficacy as a bioshield against plant fungal pathogens.
abstract: Chitinases have been suggested to be involved in pathogen-antagonist interaction during biological control progress of plant pathogenic fungi. Here, a recombinant bacterial chitinase originally from Serratia marcescens B4A was produced, purified, and assayed biochemically to ascertain the activity and determine the kinetics parameters. Active enzyme was used to determine its biocontrol features against fungal phytopathogens. The results demonstrated that the optimum pH and temperature for the enzyme activity were 6.0 and 55 °C, respectively. The K(m) and V(max) values were 3.30 mg ml(-1) and 0.92 units, respectively. The recombinant chitinase was demonstrated to be highly active in controlling fungal pathogens.
authors.affiliation: National Institute of Genetic Engineering and Biotechnology (NIGEB), Shahrak-e Pajoohesh, Km 15 Tehran-Karaj Highway, PO Box 14155-6343, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Babashpour
authors.forename: S
authors.initials: S
authors.lastname: Aminzadeh
authors.forename: N
authors.initials: N
authors.lastname: Farrokhi
authors.forename: A
authors.initials: A
authors.lastname: Karkhane
authors.forename: K
authors.initials: K
authors.lastname: Haghbeen
mesh_terms: D000528:Alternaria / Q000187:drug effects / Q000254:growth & development; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D019295:Computational Biology; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D057075:Enzyme Assays; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D012232:Rhizoctonia / Q000187:drug effects / Q000254:growth & development; D012706:Serratia marcescens / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; D001688:Biological Products; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D002686:Chitin; D002688:Chitinases