title: Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation.
abstract: Osteoporosis arises from an imbalance between osteoblastic bone formation and osteoclastic bone resorption. In this study, we screened molecules from marine natural products that stimulate osteoblast differentiation. We found that phorbaketal A significantly stimulates osteoblast differentiation in mesenchymal cells. Increased interaction of TAZ and Runx2 stimulated phorbaketal A-induced expression of osteoblastic marker genes. The activation of ERK was important for the stimulation of differentiation because an inhibitor of ERK blocked phorbaketal A-induced osteogenic differentiation. Taken together, the results showed that phorbaketal A stimulates TAZ-mediated osteoblast differentiation through the activation of ERK.
authors.affiliation: School of Life Science and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Mi Ran
authors.initials: MR
authors.lastname: Byun
authors.forename: A Rum
authors.initials: AR
authors.lastname: Kim
authors.forename: Jun-Ha
authors.initials: JH
authors.lastname: Hwang
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Sung
authors.forename: Yeon Kyung
authors.initials: YK
authors.lastname: Lee
authors.forename: Buyng Su
authors.initials: BS
authors.lastname: Hwang
authors.forename: Jung-Rae
authors.initials: JR
authors.lastname: Rho
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Hwang
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Hong
mesh_terms: D000217:Acyltransferases; D000818:Animals; D002454:Cell Differentiation; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D054830:Sesterterpenes / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C493716:Runx2 protein, mouse; D054830:Sesterterpenes; D014157:Transcription Factors; C546752:phorbaketal A; D000217:Acyltransferases; C486363:tafazzin protein, mouse; D048049:Extracellular Signal-Regulated MAP Kinases