title: Anti-aging effects of deuterium depletion on Mn-induced toxicity in a C. elegans model.
abstract: Work with sub-natural levels of deuterium (D) in animals has demonstrated an anti-cancer effect of low D-concentration in water. Our objective was to investigate whether deuterium-depleted water (DDW) can overturn reverse manganese (Mn)-induced reduction in life span, using the Caenorhabditis elegans (C. elegans) as a model system. DDW per se had no effect on worm's life span 48 h after treatment; however, it reversed the Mn-induced decrease in C. elegans life span. Mn reduced DAF-16 levels, a transcription factor strongly associated with life-span regulation. Low D-concentration (90 ppm) restored the Mn-induced changes in DAF-16 to levels indistinguishable from controls, suggesting DDW can regulate the DAF-16 pathway. We further show that insulin-like receptor DAF-2 levels were unaltered by Mn exposure, tAKT levels increased, whilst superoxide dismutase (SOD-3) levels were decreased by Mn. DDW (90 ppm) restored the levels of tAKT and superoxide dismutase (SOD) to control values without changing DAF-2 levels. Treatment of Mn exposed worms with DDW (90 ppm) restored life-span, DAF-16 and SOD-3 levels to control levels, strongly suggesting that low D concentrations can protect against Mn toxic effects.
authors.affiliation: Universidade Federal do Pampa, BR 472 Km 585, CEP 97500-970, Uruguaiana, RS, Brazil.
authors.forename: Daiana Silva
authors.initials: DS
authors.lastname: Avila
authors.forename: Gábor
authors.initials: G
authors.lastname: Somlyai
authors.forename: Ildikó
authors.initials: I
authors.lastname: Somlyai
authors.forename: Michael
authors.initials: M
authors.lastname: Aschner
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D003903:Deuterium / Q000633:toxicity*; D051858:Forkhead Transcription Factors; D008136:Longevity / Q000187:drug effects; D020149:Manganese Poisoning / Q000473:pathology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D016019:Survival Analysis; D014157:Transcription Factors / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D029742:Caenorhabditis elegans Proteins; D051858:Forkhead Transcription Factors; D014157:Transcription Factors; C108831:daf-16 protein, C elegans; D014867:Water; D003903:Deuterium; D051057:Proto-Oncogene Proteins c-akt