title: The interplay between the disulfide bond formation pathway and cytochrome c maturation in Escherichia coli.
abstract: Heme attachment to c-type cytochromes in bacteria requires cysteine thiols in the CXXCH motif of the protein. The involvement of the periplasmic disulfide generation system in this process remains unclear. We undertake a systematic evaluation of the role of DsbA and DsbD in cytochrome c biogenesis in Escherichia coli and show unequivocally that DsbA is not essential for holocytochrome production under aerobic or anaerobic conditions. We also prove that DsbD is important but not essential for maturation of c-type cytochromes. We discuss the findings in the context of a model in which heme attachment to, and oxidation of, the apocytochrome are competing processes.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.
authors.forename: Despoina A I
authors.initials: DA
authors.lastname: Mavridou
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Ferguson
authors.forename: Julie M
authors.initials: JM
authors.lastname: Stevens
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003553:Cystine / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D055786:Gene Knockout Techniques; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D019897:Periplasm / Q000378:metabolism; D019704:Protein Disulfide-Isomerases / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C079503:DsbB protein, Bacteria; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D003553:Cystine; D045304:Cytochromes c; D010088:Oxidoreductases; C094848:DsbD electron transport protein, E coli; D019704:Protein Disulfide-Isomerases; C526648:dsbA protein, E coli; D010100:Oxygen