title: Activation of the mammalian cells by using light-sensitive ion channels.
abstract: With advances in molecular biology and gene cloning techniques, it is now possible to selectively stimulate living cells of interest by using an external light source. This is done by transfecting the cells of interest with a plasmid carrying the channelrhodopsin (ChR2) gene. By stimulating these transfected cells with laser, the light-sensitive ion channels ChR2 are opened, followed by an influx of cation resulting in cell activation. This combination of optical and genetic technique is known in the literature as optogenetics. It is particularly useful in the functional studies of excitable cells, such as neurons, muscle and endocrine cells, to mimic the stimulation from action potentials to trigger the release neurotransmitters and hormones. Here, we describe the methods needed to make selected mammalian cells (PC12) respond to light excitation.
authors.affiliation: Australian School of Advanced Medicine, Macquarie University, North Ryde, NSW, Australia.
authors.forename: Mandy Siu Yu
authors.initials: MS
authors.lastname: Lung
authors.forename: Paul
authors.initials: P
authors.lastname: Pilowsky
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Goldys
mesh_terms: D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000379:methods; D016716:PC12 Cells; D010957:Plasmids; D051381:Rats; D012243:Rhodopsin / Q000378:metabolism*; D014162:Transfection; D014169:Transformation, Bacterial
chemical_list: D007473:Ion Channels; D012243:Rhodopsin; D002118:Calcium