title: Effect of HSPC016 gene expression on the aggregative growth of dermal papillae cells.
abstract: In previous studies, HSPC016 was identified as a differentially expressed gene in dermal papilla cells (DPC) with aggregative behaviour. To clarify its role in the regulation of DPC, the recombinant HSPC016 protein was expressed using the Pichia pastoris expression system, and three small interfering ribonucleic acid duplexes (siRNA) were designed and transfected into DPC. Results showed that DPC with the HSPC016 gene inhibited, exhibit non-aggregative behaviour and grow much slower than normal DPC, and that the recombinant HSPC016 protein could promote the proliferation of high-passage DPC and induce its aggregative behaviour.
authors.affiliation: Department of Dermatology, Southwest Hospital, Chongqing, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Song
authors.forename: Chunli
authors.initials: C
authors.lastname: Zhou
authors.forename: Jiwen
authors.initials: J
authors.lastname: Wang
authors.forename: Weibin
authors.initials: W
authors.lastname: Yang
authors.forename: Fei
authors.initials: F
authors.lastname: Hao
mesh_terms: D002449:Cell Aggregation / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D015870:Gene Expression; D018859:Hair Follicle / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D011506:Proteins / Q000235:genetics*
chemical_list: D011506:Proteins; C502622:TMA7 protein, human