title: Liposomes from hydrogenated soya lecithin formed in sintered glass pores.
abstract: Possible complete closure of hydrophilic drug solutions in liposomes with required dimensions is the aim of variety liposome techniques. The ease of separating medication-loaded liposomes from liposome suspension to achieve an appropriate drug concentration in the final preparation is also desired. This paper describes the use of liposome preparation method, called reverse-phase evaporation, which leads to practical achievement of the earlier mentioned objectives. Preparation process is performed in an appropriately designed device. In optimal conditions of liposome preparation the final encapsulation efficiency of hydrophilic drug solution amounted to 50% in liposomes with a diameter in the range of a few micrometers up to 250 nm. The diameter of terminal liposomes is a simple function of relative amount of the lipid used and the degree of emulsion emulsification w/o at the beginning of liposome preparation. The density of the concentrated drug solution trapped in liposomes is usually higher than that of the buffer. Therefore, the loaded liposomes may be easily separated from non-trapped material by using of a simple sedimentation at 30000 x g. Density of aqueous drug solution insufficient to effective centrifugation can be magnified with an appropriate quantity of sucrose solution before encapsulation.
authors.affiliation: Department of Physical Pharmacy, Medical University of Silesia, 4, Jagiellońska St., 41-200 Sosnowiec, Poland. zzawada@sum.edu.pl
authors.forename: Zygmunt H
authors.initials: ZH
authors.lastname: Zawada
mesh_terms: D002626:Chemistry, Pharmaceutical; D004339:Drug Compounding; D004867:Equipment Design; D005898:Glass / Q000737:chemistry*; D006865:Hydrogenation; D057927:Hydrophobic and Hydrophilic Interactions; D054709:Lecithins / Q000737:chemistry* / Q000302:isolation & purification; D008081:Liposomes; D010316:Particle Size; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D016062:Porosity; D011312:Pressure; D012997:Solvents / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000295:instrumentation* / Q000379:methods; D014461:Ultracentrifugation
chemical_list: D054709:Lecithins; D008081:Liposomes; D004364:Pharmaceutical Preparations; D012997:Solvents