title: Design and fabrication of N-alkyl-polyethylenimine-stabilized iron oxide nanoclusters for gene delivery.
abstract: With the rapid development of nanotechnology, inorganic magnetic nanoparticles, especially iron oxide nanoparticles (IOs), have emerged as great vehicles for biomedical diagnostic and therapeutic applications. In order to rationally design IO-based gene delivery nanovectors, surface modification is essential and determines the loading and release of the gene of interest. Here we highlight the basic concepts and applications of nonviral gene delivery vehicles based on low molecular weight N-alkyl polyethylenimine-stabilized IOs. The experimental protocols related to these topics are described in this chapter.
authors.affiliation: Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
authors.forename: Seulki
authors.initials: S
authors.lastname: Lee
authors.forename: Hua
authors.initials: H
authors.lastname: Ai
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D005290:Ferric Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D018014:Gene Transfer Techniques; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D018625:Microscopy, Atomic Force; D053761:Nanocomposites / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry* / Q000633:toxicity; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
chemical_list: D005290:Ferric Compounds; D034741:RNA, Small Interfering; C000499:ferric oxide; D011094:Polyethyleneimine