title: Characterization of calcium, phosphate and peroxide interactions in activation of mitochondrial swelling using derivative of the swelling curves.
abstract: We describe a new method for the analysis of mitochondrial swelling curves. Using classical swelling curves, only the maximum extent of the swelling can be calculated in a numerical form. However, taking the derivative of the classical swelling curves enables the evaluation of two additional parameters of the swelling process in a numerical form, namely, the maximum swelling rate after the addition of the swelling inducer (as dA₅₂₀/10 s) and the time (in sec) at which the maximum swelling rate after the addition of the swelling inducer is obtained. The use of these three parameters enables the better characterization of the swelling process as demonstrated by the evaluation of calcium and phosphate interactions in the opening of the mitochondrial permeability transition pore and by the characterization of the peroxide potentiating action.
authors.affiliation: Institute of Physiology and Center for Applied Genomics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Drahota
authors.forename: René
authors.initials: R
authors.lastname: Endlicher
authors.forename: Pavla
authors.initials: P
authors.lastname: Staňková
authors.forename: David
authors.initials: D
authors.lastname: Rychtrmoc
authors.forename: Marie
authors.initials: M
authors.lastname: Milerová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Cervinková
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D008933:Mitochondrial Swelling / Q000502:physiology*; D010539:Permeability; D010545:Peroxides / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D010545:Peroxides; D010710:Phosphates; D002118:Calcium