title: A specific molecular beacon probe for the detection of human prostate cancer cells.
abstract: The small-molecule, water-soluble molecular beacon probe 1 is hydrolyzed by the lysate and living cells of human prostate cancer cell lines (LNCaP), resulting in strong green fluorescence. In contrast, probe 1 does not undergo significant hydrolysis in either the lysate or living cells of human nontumorigenic prostate cells (RWPE-1). These results, corroborated by UV-Vis spectroscopy and fluorescent microscopy, reveal that probe 1 is a sensitive and specific fluorogenic and chromogenic sensor for the detection of human prostate cancer cells among nontumorigenic prostate cells and that carboxylesterase activity is a specific biomarker for human prostate cancer cells.
authors.affiliation: Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN 37614, United States. jiangy@etsu.edu
authors.forename: Yu Lin
authors.initials: YL
authors.lastname: Jiang
authors.forename: Christopher A
authors.initials: CA
authors.lastname: McGoldrick
authors.forename: Deling
authors.initials: D
authors.lastname: Yin
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Vini
authors.initials: V
authors.lastname: Patel
authors.forename: Marianne F
authors.initials: MF
authors.lastname: Brannon
authors.forename: Janet W
authors.initials: JW
authors.lastname: Lightner
authors.forename: Koyamangalath
authors.initials: K
authors.lastname: Krishnan
authors.forename: William L
authors.initials: WL
authors.lastname: Stone
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D043182:Carboxylesterase / Q000378:metabolism; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006860:Hydrogen Bonding; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D015335:Molecular Probes / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D015335:Molecular Probes; D043182:Carboxylesterase