title: Reactive gliosis and neuroinflammation in rats with communicating hydrocephalus.
abstract: Reactive gliosis has been implicated in injury and recovery patterns associated with hydrocephalus. The roles that these mechanisms play in the pathophysiology of hydrocephalus are still not clear in terms of cytopathology and gene expression. In this paper, we investigated the relationship between reactive gliosis and neuroinflammation of hydrocephalic rats of different severity at both cellular and molecular levels. Therefore 35 adult SD (standard deviation) rats were randomly divided into the normal group (n=5), the sham operation group (n=5) and the model group (n=25). Hydrocephalic rat models were induced by intraventricular injections of 3% kaolin, and the ventricular dilatation was examined by MRI (magnetic resonance imaging) at 2-week postoperation. Then the model group was subdivided into the mild group (n=5), the moderate group (n=7) and the severe group (n=9) according to the degree of ventricular dilatation. While IL-18 (interlukin 18), GFAP (glial fibrillary acidic protein), and Iba-1 (ionized calcium binding adaptor molecule-1) were detected by ELISA (enzyme-linked immunosorbent assay), RT-PCR, immunohistochemistry, Western blot and correlation analysis were conducted at the same time. According to the result comparison between the normal group and the sham operation group, the ventricle of model group was obviously enlarged (P<0.01). The expression of GFAP and Iba-1 was increased (P<0.05) in brain tissue of the model group and IL-18 was also increased in CSF (cerebrosinal fluid) sample of model group. It was revealed by correlation analysis that the increase was positively correlated with the severity of ventricular dilatation.
  
  
  CONCLUSIONS
  These results indicate that gliosis and inflammation continue to rise dramatically in experimental hydrocephalus and can be regarded as the main factors of hydrocephalus. Regulating the level of gliosis and alleviating inflammation may provide new therapeutic methods of hydrocephalus.
authors.affiliation: Department of Neurosurgery, First Affiliate Hospital of Xiamen University, Xiamen, Fujian Province 361003, China.
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.forename: S L
authors.initials: SL
authors.lastname: Zhang
authors.forename: G W
authors.initials: GW
authors.lastname: Tan
authors.forename: H W
authors.initials: HW
authors.lastname: Zhu
authors.forename: C Q
authors.initials: CQ
authors.lastname: Huang
authors.forename: F F
authors.initials: FF
authors.lastname: Zhang
authors.forename: Z X
authors.initials: ZX
authors.lastname: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005911:Gliosis / Q000473:pathology*; D006849:Hydrocephalus / Q000473:pathology* / Q000503:physiopathology*; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction