title: Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis.
abstract: The first step during bacillithiol (BSH) biosynthesis involves the formation of N-acetylglucosaminylmalate from UDP-N-acetylglucosamine and l-malate and is catalyzed by a GT4 class glycosyltransferase enzyme (BshA). Recombinant Staphylococcus aureus and Bacillus subtilis BshA were highly specific and active with l-malate but the former showed low activity with d-glyceric acid and the latter with d-malate. We show that BshA is inhibited by BSH and similarly that MshA (first enzyme of mycothiol biosynthesis) is inhibited by the final product MSH.
authors.affiliation: Department of Biology, California State University-Fresno, Fresno, CA 93740, USA.
authors.forename: Heather
authors.initials: H
authors.lastname: Upton
authors.forename: Gerald L
authors.initials: GL
authors.lastname: Newton
authors.forename: Melissa
authors.initials: M
authors.lastname: Gushiken
authors.forename: Kelly
authors.initials: K
authors.lastname: Lo
authors.forename: Dhiraj
authors.initials: D
authors.lastname: Holden
authors.forename: Robert C
authors.initials: RC
authors.lastname: Fahey
authors.forename: Mamta
authors.initials: M
authors.lastname: Rawat
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D016863:Bacillaceae Infections / Q000188:drug therapy; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000378:metabolism; D006020:Glycopeptides / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D007700:Kinetics; D008293:Malates / Q000378:metabolism; D008958:Models, Molecular; D058990:Molecular Targeted Therapy; D008970:Molecular Weight; D017351:N-Acetylglucosaminyltransferases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000201:enzymology*; D013379:Substrate Specificity; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D006020:Glycopeptides; D008293:Malates; D011994:Recombinant Proteins; C543521:bacillithiol; C089265:mycothiol; D007294:Inositol; D014537:Uridine Diphosphate N-Acetylglucosamine; C030298:malic acid; D017351:N-Acetylglucosaminyltransferases; D003545:Cysteine; D005944:Glucosamine