title: Optimization of cold-adapted lysozyme production from the psychrophilic yeast Debaryomyces hansenii using statistical experimental methods.
abstract: Statistical experimental designs were employed to optimize culture conditions for cold-adapted lysozyme production of a psychrophilic yeast Debaryomyces hansenii. In the first step of optimization using Plackett-Burman design (PBD), peptone, glucose, temperature, and NaCl were identified as significant variables that affected lysozyme production, the formula was further optimized using a four factor central composite design (CCD) to understand their interaction and to determine their optimal levels. A quadratic model was developed and validated. Compared to the initial level (18.8 U/mL), the maximum lysozyme production (65.8 U/mL) observed was approximately increased by 3.5-fold under the optimized conditions.
  
  
  CONCLUSIONS
  Cold-adapted lysozymes production was first optimized using statistical experimental methods. A 3.5-fold enhancement of microbial lysozyme was gained after optimization. Such an improved production will facilitate the application of microbial lysozyme. Thus, D. hansenii lysozyme may be a good and new resource for the industrial production of cold-adapted lysozymes.
authors.affiliation: School of Marine and Technology, Harbin Inst of Technology, Weihai 264209, PR China.
authors.forename: Quanfu
authors.initials: Q
authors.lastname: Wang
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Hou
authors.forename: Peisheng
authors.initials: P
authors.lastname: Yan
mesh_terms: D000864:Antarctic Regions; D003080:Cold Temperature; D055165:Debaryomyces / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D005285:Fermentation; D005520:Food Preservatives / Q000378:metabolism*; D005524:Food Technology; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D009113:Muramidase / Q000096:biosynthesis* / Q000378:metabolism; D009994:Osmolar Concentration; D010461:Peptones / Q000378:metabolism; D012623:Seawater / Q000382:microbiology; D012965:Sodium Chloride / Q000378:metabolism; D013223:Statistics as Topic
chemical_list: D005520:Food Preservatives; D005656:Fungal Proteins; D010461:Peptones; D012965:Sodium Chloride; D009113:Muramidase; D005947:Glucose