title: N-Glycolyl GM3 ganglioside immunoexpression in oral mucosal melanomas of Chinese.
abstract: OBJECTIVE
  The aim of this study was to determine the expression of N-Glycolyl GM3 (NeuGcGM3) ganglioside in oral mucosal melanomas.
  
  
  METHODS
  To assess the presence of cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) mRNA, an RT-PCR assay was performed in melanoma cell line (ME), an oral mucosal ME, and two fresh oral mucosal melanoma tissues. Expression of NeuGcGM3 ganglioside was evaluated by immunohistochemistry in 44 primary oral mucosal melanomas and 10 oral melanotic nevi. Also, the expression of NeuGcGM3 was examined in ME by immunocytochemistry.
  
  
  RESULTS
  We first checked the expression of CMAH in ME and two fresh oral mucosal melanoma tissues. Presence of NeuGcGM3 ganglioside was evident in 37 of 44 cases (84.1%), showing a diffuse cytoplasmic and membranous staining. Patients with primary occurrence showed high levels of NeuGcGM3 ganglioside compared to patients with secondary occurrence. On the other hand, negative immunoreaction was evidenced in oral melanotic nevi. ME also presented the expression of NeuGcGM3 by immunocytochemistry.
  
  
  CONCLUSIONS
  In this work, we for the first time evaluated the expression of 14F7 MAb immunorecognition in oral mucosal melanomas. Our results were in agreement with the assumption that NeuGcGM3 ganglioside may be considered as target for passive and active immunotherapy in oral mucosal melanomas expressing this molecule and indicate less risk of recurrence and a better prognosis. Moreover, ME provides a platform for more studies on the specific function of NeuGcGM3 in oral mucosal melanomas.
authors.affiliation: Shanghai Key Laboratory of Stomatology, Department of Oral & Maxillofacial-Head & Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhong
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Tang
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Ren
authors.forename: A
authors.initials: A
authors.lastname: Carr
authors.forename: R
authors.initials: R
authors.lastname: Pérez
authors.forename: W
authors.initials: W
authors.lastname: Guo
mesh_terms: D000911:Antibodies, Monoclonal; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000648:ultrastructure; D002681:China; D003593:Cytoplasm / Q000648:ultrastructure; D005260:Female; D005679:G(M3) Ganglioside / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G; D007150:Immunohistochemistry; D008297:Male; D008545:Melanoma / Q000473:pathology* / Q000556:secondary; D008875:Middle Aged; D006899:Mixed Function Oxygenases / Q000032:analysis; D009061:Mouth Mucosa / Q000473:pathology*; D009062:Mouth Neoplasms / Q000473:pathology*; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D009508:Nevus, Pigmented / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D005679:G(M3) Ganglioside; D007074:Immunoglobulin G; C044298:N-glycolylneuraminyllactosylceramide; D006899:Mixed Function Oxygenases; C074383:CMPacetylneuraminate monooxygenase