title: Synthesis of a ratiometric fluorescent peptide sensor for the highly selective detection of Cd2+.
abstract: A novel ratiometric fluorescent peptidyl chemosensor (Dansyl-Cys-Pro-Gly-Cys-Trp-NH(2), D-P5) for metal ions detection has been synthesized via Fmoc solid-phase peptide synthesis. The chemosensor exhibited a high selectivity for Cd(2+) over other metal ions including competitive transition and Group I and II metal ions in neutral pH. The fluorescence emission intensity of D-P5 was significantly enhanced in the presence of Cd(2+) by fluorescent resonance energy transfer (FRET) and chelation enhanced fluorescence (CHEF) effects. The binding stoichiometry, detection limit, binding affinity, reversibility and pH sensitivity of the sensor for Cd(2+) were investigated.
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Lianzhi
authors.initials: L
authors.lastname: Li
authors.forename: Xuewei
authors.initials: X
authors.lastname: Pu
authors.forename: Guolin
authors.initials: G
authors.lastname: Ma
authors.forename: Erqiong
authors.initials: E
authors.lastname: Wang
authors.forename: Jinming
authors.initials: J
authors.lastname: Kong
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Liu
authors.forename: Yangzhong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D002104:Cadmium / Q000032:analysis*; D002413:Cations, Divalent; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis*; D010713:Phosphatidylcholines; D011485:Protein Binding; D012680:Sensitivity and Specificity; D060327:Solid-Phase Synthesis Techniques; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: C496906:1-myristoyl-2-(12-((5-dimethylamino-1-naphthalenesulfonyl)amino)dodecanoyl)-sn-glycero-3-phosphocholine; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D010455:Peptides; D010713:Phosphatidylcholines; D014874:Water Pollutants, Chemical; D002104:Cadmium