title: Epigenetic inactivation of PLCD1 in chronic myeloid leukemia.
abstract: Phospholipase C δ1 (PLCD1), is located at the important tumor suppressor             locus 3p22. It encodes an enzyme that mediates regulatory signaling of energy             metabolism, calcium homeostasis and intracellular movements. PLCD1 has been studied             in some human solid tumors relating to the CpG island methylation of the gene             promoter as a functional tumor suppressor. However, no such information is available             in chronic myeloid leukemia (CML). In this study, we investigated PLCD1 expression             in the CML K562 cell line (0/1) and 15% (2/13) of bone marrow mononuclear cells             with CML by using semi-quantitative PCR. The CpG island (CGI) methylation status             of the PLCD1 promoter was detected in K562 (0/1) and 56% (23/41) of CML patients             by methylation-specific PCR (MSP), but not in the normal adult bone marrow mononuclear             cells. Furthermore, the DNA demethylation agent 5'-aza-2'deoxycytidine restored             the expression of PLCD1 in K562 cells. Functional studies showed that ectopic             expression of PLCD1 in K562 cells was able to dramatically inhibit their colony             formation and induce cell cycle G1 arrest, suggesting that PLCD1 acts as a functional             tumor suppressor and may serve as a biomarker for possible early detection and             prognosis of CML.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Chongqing                     Medical University, Chongqing, P.R. China.
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Song
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Ze-Song
authors.initials: ZS
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Ting-Xiu
authors.initials: TX
authors.lastname: Xiang
authors.forename: Guo-Sheng
authors.initials: GS
authors.lastname: Ren
authors.forename: Jian-Bin
authors.initials: JB
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000235:genetics; D001854:Bone Marrow Cells; D018899:CpG Islands; D019175:DNA Methylation; D000077209:Decitabine; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D054803:Phospholipase C delta / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; D000077209:Decitabine; C518024:PLCD1 protein, human; D054803:Phospholipase C delta; D001374:Azacitidine