title: Deep 16S rRNA metagenomics and quantitative PCR analyses of the premature infant fecal microbiota.
abstract: Metagenomic studies on the gut microbiota of preterm infants are scarce. We characterized the microbiota of 10 days-old neonates by deep 16S rRNA gene metagenomic analysis and compare the results with those obtained by qPCR. Both techniques lead to similar conclusions, allowing differentiating between preterm and full-term infants.
authors.affiliation: Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Villaviciosa, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Arboleya
authors.forename: Li
authors.initials: L
authors.lastname: Ang
authors.forename: Abelardo
authors.initials: A
authors.lastname: Margolles
authors.forename: Li
authors.initials: L
authors.lastname: Yiyuan
authors.forename: Zhang
authors.initials: Z
authors.lastname: Dongya
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Solís
authors.forename: Nuria
authors.initials: N
authors.lastname: Fernández
authors.forename: Clara G
authors.initials: CG
authors.lastname: de Los Reyes-Gavilán
authors.forename: Miguel
authors.initials: M
authors.lastname: Gueimonde
mesh_terms: D001644:Bifidobacterium / Q000235:genetics; D004755:Enterobacteriaceae / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D019102:Infant, Very Low Birth Weight; D007777:Lactobacillaceae / Q000235:genetics; D008297:Male; D054892:Metagenome; D056186:Metagenomics; D058889:Molecular Typing; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013289:Streptococcaceae / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S