title: Polymer-based scaffold designs for in situ vascular tissue engineering: controlling recruitment and differentiation behavior of endothelial colony forming cells.
abstract: In situ vascular tissue engineering has been proposed as a promising approach to fulfill the need for small-diameter blood vessel substitutes. The approach comprises the use of a cell-free instructive scaffold to guide and control cell recruitment, differentiation, and tissue formation at the locus of implantation. Here we review the design parameters for such scaffolds, with special emphasis on differentiation of recruited ECFCs into the different lineages that constitute the vessel wall. Next to defining the target properties of the vessel, we concentrate on the target cell source, the ECFCs, and on the environmental control of the fate of these cells within the scaffold. The prospects of the approach are discussed in the light of current technical and biological hurdles.
authors.affiliation: Soft Tissue Biomechanics and Tissue Engineering, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB Eindhoven, The Netherlands. e.s.fioretta@tue.nl
authors.forename: Emanuela S
authors.initials: ES
authors.lastname: Fioretta
authors.forename: Joost O
authors.initials: JO
authors.lastname: Fledderus
authors.forename: Ewelina A
authors.initials: EA
authors.lastname: Burakowska-Meise
authors.forename: Frank P T
authors.initials: FP
authors.lastname: Baaijens
authors.forename: Marianne C
authors.initials: MC
authors.lastname: Verhaar
authors.forename: Carlijn V C
authors.initials: CV
authors.lastname: Bouten
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000503:physiopathology / Q000637:transplantation; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D002465:Cell Movement / Q000187:drug effects; D018925:Chemokines / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D019344:Lactic Acid / Q000138:chemical synthesis / Q000494:pharmacology*; D011100:Polyglycolic Acid / Q000138:chemical synthesis / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D018925:Chemokines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid