title: High-yield production of a stable Vero cell-based vaccine candidate against the highly pathogenic avian influenza virus H5N1.
abstract: Highly pathogenic avian influenza (HPAI) viruses pose a global pandemic threat, for which rapid large-scale vaccine production technology is critical for prevention and control. Because chickens are highly susceptible to HPAI viruses, the supply of chicken embryos for vaccine production might be depleted during a virus outbreak. Therefore, developing HPAI virus vaccines using other technologies is critical. Meeting vaccine demand using the Vero cell-based fermentation process has been hindered by low stability and yield. In this study, a Vero cell-based HPAI H5N1 vaccine candidate (H5N1/YNVa) with stable high yield was achieved by reassortment of the Vero-adapted (Va) high growth A/Yunnan/1/2005(H3N2) (YNVa) virus with the A/Anhui/1/2005(H5N1) attenuated influenza vaccine strain (H5N1delta) using the 6/2 method. The reassorted H5N1/YNVa vaccine maintained a high hemagglutination (HA) titer of 1024. Furthermore, H5N1/YNVa displayed low pathogenicity and uniform immunogenicity compared to that of the parent virus.
authors.affiliation: No. 5, Department of Bioproducts, Institute of Medical Biology, Chinese Academy of Medical Science and Pecking Union Medical College, Jiaoling Avenue 935, Kunming, Yunnan Province 650102, People's Republic of China.
authors.forename: Fangye
authors.initials: F
authors.lastname: Zhou
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.forename: Shaohui
authors.initials: S
authors.lastname: Song
authors.forename: Xinwen
authors.initials: X
authors.lastname: Zhang
authors.forename: Weidong
authors.initials: W
authors.lastname: Li
authors.forename: Shude
authors.initials: S
authors.lastname: Jiang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Guoyang
authors.initials: G
authors.lastname: Liao
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000276:immunology*; D007251:Influenza, Human / Q000517:prevention & control* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D016865:Reassortant Viruses / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity; D014613:Vaccines, Attenuated / Q000276:immunology; D014709:Vero Cells; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D007252:Influenza Vaccines; D014613:Vaccines, Attenuated