title: Is cardiac tissue more susceptible than lung to oxidative effects induced by chronic nasotropic instillation of residual oil fly ash (ROFA)?
abstract: The current study aimed to determine the role of oxidants in cardiac and pulmonary toxicities induced by chronic exposure to ROFA. Eighty Wistar rats were divided into four groups: G1 (10 µL Saline), G2 (ROFA 50 µg/10 µL), G3 (ROFA 250 µg/10 µL) and G4 (ROFA 500 µg/10 µL). Rats received ROFA by nasotropic instillation for 90 days. After that, they were euthanized and bronchoalveolar lavage (BAL) was performed for total count of leukocytes, protein and lactate dehydrogenase (LDH) determinations. Lungs and heart were removed to measure lipid peroxidation (MDA), catalase (CAT) and superoxide dismutase (SOD) activity. BAL presented an increase in leukocytes count in G4 in comparison to the Saline group (p = 0.019). In lung, MDA level was not modified by ROFA, while CAT was higher in G4 when compared to all other groups (p = 0.013). In heart, G4 presented an increase in MDA (p = 0.016) and CAT (p = 0.027) levels in comparison to G1. The present study demonstrated cardiopulmonary oxidative changes after a chronic ROFA exposure. More specifically, the heart tissue seems to be more susceptible to oxidative effects of long-term exposure to ROFA than the lung.
authors.affiliation: Laboratório de Estresse Oxidativo e Poluição Atmosférica, Universidade Federal de Ciências da Saúde de Porto Alegre, Brasil. marquesdamiani@gmail.com
authors.forename: Roberto Marques
authors.initials: RM
authors.lastname: Damiani
authors.forename: Marcella Ody
authors.initials: MO
authors.lastname: Piva
authors.forename: Marcelo Rafael
authors.initials: MR
authors.lastname: Petry
authors.forename: Paulo Hilário Nascimento
authors.initials: PH
authors.lastname: Saldiva
authors.forename: Alexandre
authors.initials: A
authors.lastname: Tavares Duarte de Oliveira
authors.forename: Cláudia Ramos
authors.initials: CR
authors.lastname: Rhoden
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002374:Catalase / Q000378:metabolism; D060729:Coal Ash / Q000633:toxicity*; D003627:Data Interpretation, Statistical; D004305:Dose-Response Relationship, Drug; D006321:Heart / Q000187:drug effects*; D007322:Instillation, Drug; D007958:Leukocyte Count; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011014:Pneumonia / Q000139:chemically induced / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D036401:Toxicity Tests, Chronic
chemical_list: D000393:Air Pollutants; D060729:Coal Ash; D002374:Catalase; D013482:Superoxide Dismutase