title: Migration of monocytes after intracerebral injection.
abstract: Recently, we monitored green fluorescent protein (GFP)-expressing monocytes after injection at the entorhinal cortex lesion (ECL) site in mice. We followed their migration out of the central nervous system (CNS) along olfactory nerve fibers penetrating the lamina cribrosa, within the nasal mucosa, and their subsequent appearance within the deep cervical lymph nodes (CLN), with numbers peaking at day 7. This is the same route activated T cells use for reaching the CLN, as we have shown before. Interestingly, GFP cells injected into the brain and subsequently found in the CLN exhibited ramified morphologies, which are typical of microglia and dendritic cells. To gain more insight into immunity and regeneration within the CNS we want to monitor injected monocytes using magnetic resonance imaging (MRI) after labeling with very small superparamagnetic iron oxide particles (VSOP). Due to their small size, nanoparticles have huge potential for magnetic labeling of different cell populations and their MRI tracking in vivo. So far we have verified that incubation with VSOP particles does not alter their migration pattern after ECL.
authors.affiliation: Institute of Cell Biology and Neurobiology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Kaminski
authors.forename: Ingo
authors.initials: I
authors.lastname: Bechmann
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kiwit
authors.forename: Jana
authors.initials: J
authors.lastname: Glumm
mesh_terms: D000818:Animals; D002465:Cell Movement; D058948:Cell Tracking; D002540:Cerebral Cortex / Q000166:cytology; D005290:Ferric Compounds / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D008279:Magnetic Resonance Imaging; D051379:Mice; D009000:Monocytes / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D053758:Nanoparticles / Q000737:chemistry; D013194:Staining and Labeling
chemical_list: D005290:Ferric Compounds; D049452:Green Fluorescent Proteins; C000499:ferric oxide