title: Siansivirga zeaxanthinifaciens gen. nov., sp. nov., a novel zeaxanthin-producing member of the family Flavobacteriaceae isolated from coastal seawater of Taiwan.
abstract: A strictly aerobic, Gram-negative, rod-shaped bacterium (strain CC-SAMT-1(T)) showing gliding motility was isolated from coastal seawater of China Sea, Taiwan. Strain CC-SAMT-1(T) synthesizes all-trans-zeaxanthin (6.5 ± 0.5 mg g(-1) dry biomass) as a predominant xanthophyll carotenoid. As determined by 16S rRNA gene analysis, strain CC-SAMT-1(T) shared very high sequence similarity to the members of the genera Mariniflexile (96.1-95.3%) and Gaetbulibacter (96.0-95.9%); however, it formed a distinct phyletic lineage distantly associated with Mariniflexile species. Polar lipid profile constitutes phosphatidylethanolamine, four unidentified aminolipids, four unidentified lipids, and an unidentified glycolipid. Strain CC-SAMT-1(T) contains excessive unidentified aminolipid lipid (AL2-4) and glycolipid contents, and therefore clearly distinct from Mariniflexile species. Major fatty acids (> 5% of total fatty acids) were iso-C(15:0) (14.8%), iso-C(17:0) 3-OH (11.8%), iso-C(15:1) G (10.6%), anteiso-C(15:0) (9.7%), C(16:0) (8.1%), iso-C(16:0) 3-OH (7.9%), iso-C(15:0) 3-OH (7.5%), and summed feature 3 (containing C(16:1) ω6c and/or C(16:1) ω7c) (7.5%). Menaquinone-6 (MK-6) was major respiratory quinone. DNA G+C content was 33.7 mol%. Based on polyphasic taxonomy, strain CC-SAMT-1(T) represents a novel genus and species in the family Flavobacteriaceae for which the name Siansivirga zeaxanthinifaciens gen. nov., sp. nov. is proposed. The type strain is CC-SAMT-1(T) (= BCRC 80315(T) = JCM 17682(T)).
authors.affiliation: Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Asif
authors.initials: A
authors.lastname: Hameed
authors.forename: Mariyam
authors.initials: M
authors.lastname: Shahina
authors.forename: Shih-Yao
authors.initials: SY
authors.lastname: Lin
authors.forename: Kandikere Ramaiah
authors.initials: KR
authors.lastname: Sridhar
authors.forename: Li-Sen
authors.initials: LS
authors.lastname: Young
authors.forename: Maw-Rong
authors.initials: MR
authors.lastname: Lee
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.forename: Jui-Hsing
authors.initials: JH
authors.lastname: Chou
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
mesh_terms: D001482:Base Composition; D002338:Carotenoids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005798:Genes, Bacterial; D020459:Genes, rRNA; D006017:Glycolipids / Q000737:chemistry; D008124:Locomotion; D010641:Phenotype; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D013045:Species Specificity; D013624:Taiwan; D014871:Water Microbiology; D024341:Xanthophylls / Q000096:biosynthesis* / Q000737:chemistry; D065146:Zeaxanthins
chemical_list: D006017:Glycolipids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024341:Xanthophylls; D065146:Zeaxanthins; D002338:Carotenoids