title: Fluorescence-based biosensors.
abstract: The field of optical sensors has been a growing research area over the last three decades. A wide range of books and review articles has been published by experts in the field who have highlighted the advantages of optical sensing over other transduction methods. Fluorescence is by far the method most often applied and comes in a variety of schemes. Nowadays, one of the most common approaches in the field of optical biosensors is to combine the high sensitivity of fluorescence detection in combination with the high selectivity provided by ligand-binding proteins. In this chapter we deal with reviewing our recent results on the implementation of fluorescence-based sensors for monitoring environmentally hazardous gas molecules (e.g. nitric oxide, hydrogen sulfide). Reflectivity-based sensors, fluorescence correlation spectroscopy-based (FCS) systems, and sensors relying on the enhanced fluorescence emission on silver island films (SIFs) coupled to the total internal reflection fluorescence mode (TIRF) for the detection of gliadin and other prolamines considered toxic for celiac patients are also discussed herein.
authors.affiliation: Department of Chemistry, University of Salerno, Salerno, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Strianese
authors.forename: Maria
authors.initials: M
authors.lastname: Staiano
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ruggiero
authors.forename: Tullio
authors.initials: T
authors.lastname: Labella
authors.forename: Claudio
authors.initials: C
authors.lastname: Pellecchia
authors.forename: Sabato
authors.initials: S
authors.lastname: D'Auria
mesh_terms: D015374:Biosensing Techniques; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005903:Gliadin / Q000032:analysis; D006801:Humans; D059206:Natural Gas / Q000032:analysis; D055315:Prolamins / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D059206:Natural Gas; D055315:Prolamins; D005903:Gliadin