title: Transcriptional analysis of Arabidopsis thaliana response to lima bean volatiles.
abstract: BACKGROUND
  Exposure of plants to herbivore-induced plant volatiles (HIPVs) alters their resistance to herbivores. However, the whole-genome transcriptional responses of treated plants remain unknown, and the signal pathways that produce HIPVs are also unclear.
  
  
  RESULTS
  Time course patterns of the gene expression of Arabidopsis thaliana exposed to Lima bean volatiles were examined using Affymetrix ATH1 genome arrays. Results showed that A. thaliana received and responded to leafminer-induced volatiles from Lima beans through up-regulation of genes related to the ethylene (ET) and jasmonic acid pathways. Time course analysis revealed strong and partly qualitative differences in the responses between exposure at 24 and that at 48 h. Further experiments using either A. thaliana ET mutant ein2-1 or A. thaliana jasmonic acid mutant coi1-2 indicated that both pathways are involved in the volatile response process but that the ET pathway is indispensable for detecting volatiles. Moreover, transcriptional comparisons showed that plant responses to larval feeding do not merely magnify the volatile response process. Finally, (Z)-3-hexen-ol, ocimene, (3E)-4,8-dimethyl-1,3,7-nonatriene, and (3E,7E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene triggered responses in A. thaliana similar to those induced by the entire suite of Lima bean volatiles after 24 and 48 h.
  
  
  CONCLUSIONS
  This study shows that the transcriptional responses of plants to HIPVs become stronger as treatment time increases and that ET signals are critical during this process.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Zhang
authors.forename: Jianing
authors.initials: J
authors.lastname: Wei
authors.forename: Le
authors.initials: L
authors.lastname: Kang
mesh_terms: D000475:Alkenes / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D003517:Cyclopentanes / Q000737:chemistry / Q000378:metabolism; D004175:Diptera / Q000502:physiology; D005030:Ethylenes / Q000737:chemistry / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D000441:Hexanols / Q000737:chemistry / Q000494:pharmacology; D007814:Larva / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins / Q000737:chemistry / Q000378:metabolism; D027805:Phaseolus / Q000737:chemistry* / Q000378:metabolism / Q000469:parasitology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013729:Terpenes / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D055549:Volatile Organic Compounds / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C500824:4,8,12-trimethyl-1,3,7,11-tridecatetraene; C411254:4,8-dimethyl-1,3,7-nonatriene; D000475:Alkenes; D003517:Cyclopentanes; D005030:Ethylenes; D000441:Hexanols; D054883:Oxylipins; D010936:Plant Extracts; D013729:Terpenes; D055549:Volatile Organic Compounds; C051918:3-hexen-1-ol; C011006:jasmonic acid; C036216:ethylene