title: A Validated Reverse Phase HPLC Analytical Method for Quantitation of Glycoalkaloids in Solanum lycocarpum and Its Extracts.
abstract: Solanum lycocarpum (Solanaceae) is native to the Brazilian Cerrado. Fruits of this species contain the glycoalkaloids solasonine (SN) and solamargine (SM), which display antiparasitic and anticancer properties. A method has been developed for the extraction and HPLC-UV analysis of the SN and SM in different parts of S. lycocarpum, mainly comprising ripe and unripe fruits, leaf, and stem. This analytical method was validated and gave good detection response with linearity over a dynamic range of 0.77-1000.00 μg mL(-1) and recovery in the range of 80.92-91.71%, allowing a reliable quantitation of the target compounds. Unripe fruits displayed higher concentrations of glycoalkaloids (1.04% ± 0.01 of SN and 0.69% ± 0.00 of SM) than the ripe fruits (0.83% ± 0.02 of SN and 0.60% ± 0.01 of SM). Quantitation of glycoalkaloids in the alkaloidic extract gave 45.09% ± 1.14 of SN and 44.37% ± 0.60 of SM, respectively.
authors.affiliation: Laboratório de Farmacognosia, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n, 14040-903 Ribeirão Preto, SP, Brazil.
authors.forename: Renata Fabiane Jorge
authors.initials: RF
authors.lastname: Tiossi
authors.forename: Mariza Abreu
authors.initials: MA
authors.lastname: Miranda
authors.forename: João Paulo Barreto
authors.initials: JP
authors.lastname: de Sousa
authors.forename: Fabíola Silva Garcia
authors.initials: FS
authors.lastname: Praça
authors.forename: Maria Vitória Lopes Badra
authors.initials: MV
authors.lastname: Bentley
authors.forename: James Dewey
authors.initials: JD
authors.lastname: McChesney
authors.forename: Jairo Kenupp
authors.initials: JK
authors.lastname: Bastos