title: [Production of enzyme preparations on the basis of Penicillum canescens recombinant strains with a high ability for the hydrolysis of plant materials].
abstract: An enzyme preparation has been produced on the basis of Penicillium canescens strains with the activity of cellibiohydrolase I, II; endo-1,4-beta-gluconase of Penicillium verruculosum; and beta-glucosidase of Aspergillus niger. It was shown that for the most effective hydrolysis of aspen wood pulp the optimal ratio of cellobiohydrolase and endo- 1,4-3-gluconase in enzyme preparations was 8 : 2 (by protein). It was also established that the homologous xylanase secreted by the Penicillium canescens fungus is a required component for the enzyme complex for hydrolysis of the hemicellulose matrix of aspen wood.
authors.forename: P V
authors.initials: PV
authors.lastname: Volkov
authors.forename: A M
authors.initials: AM
authors.lastname: Rozhkova
authors.forename: A G
authors.initials: AG
authors.lastname: Pravil'nikov
authors.forename: R M
authors.initials: RM
authors.lastname: Andrianov
authors.forename: G S
authors.initials: GS
authors.lastname: Dotsenko
authors.forename: A O
authors.initials: AO
authors.lastname: Bekkarevich
authors.forename: A V
authors.initials: AV
authors.lastname: Koshelev
authors.forename: O N
authors.initials: ON
authors.lastname: Okunev
authors.forename: I N
authors.initials: IN
authors.lastname: Zorov
authors.forename: A P
authors.initials: AP
authors.lastname: Sinitsyn
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics; D002480:Cellulase / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D060847:Metabolic Engineering; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D011134:Polysaccharides / Q000378:metabolism; D032107:Populus / Q000737:chemistry*; D014934:Wood / Q000737:chemistry*; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides; C007916:hemicellulose; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D043366:Cellulose 1,4-beta-Cellobiosidase