title: Optimization of a microwave-coupled enzymatic digestion process to prepare peanut peptides.
abstract: The best enzyme to prepare peanut peptides, papain, coupled with microwave irradiation was selected from five common proteases according to the results of the yield of peanut peptides [nitrogen solution index (NSI) in trichloroacetic acid (TCA), TCA-NSI] and the degree of hydrolysis (DH). The main factors that influenced the microwave-coupled enzymatic digestion method were optimized by response surface analysis. The optimal conditions obtained were as follows: microwave extraction time, 9.5 min; power, 600 W; substrate concentration, 4%; enzymatic reaction temperature, 50 °C; enzyme quantity, 6,500 U/g; pH, 7.1 (phosphate buffer, 0.05 mol/L). Under these conditions, TCA-NSI was 62.00% and DH was 25.89%, which is higher than that obtained with either protease hydrolysis or microwave hydrolysis alone.
authors.affiliation: Shandong Normal University, Jingshi Road No. 167, Lixia District, Jinan 250014, China.
authors.forename: Huicui
authors.initials: H
authors.lastname: Zhang
authors.forename: Lina
authors.initials: L
authors.lastname: Yu
authors.forename: Qingli
authors.initials: Q
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Bi
authors.forename: Shaofang
authors.initials: S
authors.lastname: Liu
authors.forename: Chushu
authors.initials: C
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
mesh_terms: D010367:Arachis / Q000737:chemistry*; D005163:Factor Analysis, Statistical; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008872:Microwaves; D010206:Papain / Q000378:metabolism*; D010455:Peptides / Q000032:analysis / Q000737:chemistry*; D013696:Temperature; D014238:Trichloroacetic Acid / Q000737:chemistry
chemical_list: D010455:Peptides; D014238:Trichloroacetic Acid; D010206:Papain