title: Kinetics of rebounding of lymphoid and myeloid cells in mouse peripheral blood, spleen and bone marrow after treatment with cyclophosphamide.
abstract: Recently, we showed that post cyclophosphamide (CTX) microenvironment benefits the function of transferred T cells. Analysis of the kinetics of cellular recovery after CTX treatment showed that a single 4 mg/mouse CTX treatment decreased the absolute number of leukocytes in the peripheral blood (PBL) at days 3-15, and in the spleen and bone marrow (BM) at days 3-6. The absolute numbers of CD11c(+)CD11b(-) and CD11c(+)CD11b(+) dendritic cells (DCs), CD11b(+) and Ly6G(+) myeloid cells, T and B cells, CD4(+)CD25(+) T regulatory (T(reg)) cells, and NK1.1(+) cells also decreased. The cell numbers returned to control levels during the recovery phase. The absolute numbers of B cells remained low for 3 weeks. The numbers of DCs increased in PBL and spleen at day 9 but returned to control levels at day 15. These data indicate that CTX alters the cellular microenvironment in kinetics that might be precisely targeted to benefit the host.
authors.affiliation: Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt. mohamed.labib@science.tanta.edu.eg
authors.forename: Mohamed L
authors.initials: ML
authors.lastname: Salem
authors.forename: Amir A
authors.initials: AA
authors.lastname: Al-Khami
authors.forename: Sabry A
authors.initials: SA
authors.lastname: El-Nagaar
authors.forename: Abdel-Aziz A
authors.initials: AA
authors.lastname: Zidan
authors.forename: Ismail M
authors.initials: IM
authors.lastname: Al-Sharkawi
authors.forename: C
authors.initials: C
authors.lastname: Marcela Díaz-Montero
authors.forename: David J
authors.initials: DJ
authors.lastname: Cole
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D001853:Bone Marrow / Q000187:drug effects* / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000187:drug effects* / Q000276:immunology; D013154:Spleen / Q000187:drug effects* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D003520:Cyclophosphamide