title: MicroRNA-223 regulates FOXO1 expression and cell proliferation.
abstract: In HCT116 colorectal cancer cells, HeLa cervical cancer cells and HuH-7 hepatoma cells, miR-223 is expressed at a low level. Through infection with lentivirus containing miR-223 precursor, miR-223 [corrected] was overexpressed in all these cells. Interestingly, the expression levels of FOXO1 mRNA and protein, and phosphorylation levels became significantly lower than those of their control. FOXO1 was down-regulated mainly in the cytoplasm, while the nuclear FOXO1 level became relatively high compared to the cytoplasm. As the unphosphorylated active form of FOXO1 increased in the cells, cyclin D1/p21/p27 were up-regulated at either mRNA or protein level. Proliferation of the cells was also greatly inhibited when miR-223 was over-expressed. Therein, our data suggest that miR-223 regulates FOXO1 expression and cell proliferation.
authors.affiliation: Department of Children's Health Care, Yu Ying Children's Hospital, Wenzhou Medical College, Wenzhou, PR China. lhwu@wzmc.net
authors.forename: Lihui
authors.initials: L
authors.lastname: Wu
authors.forename: Huihui
authors.initials: H
authors.lastname: Li
authors.forename: Cheng You
authors.initials: CY
authors.lastname: Jia
authors.forename: Wei
authors.initials: W
authors.lastname: Cheng
authors.forename: Mei
authors.initials: M
authors.lastname: Yu
authors.forename: Min
authors.initials: M
authors.lastname: Peng
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Zhu
authors.forename: Qianlei
authors.initials: Q
authors.lastname: Zhao
authors.forename: Yi Wei
authors.initials: YW
authors.lastname: Dong
authors.forename: Kang
authors.initials: K
authors.lastname: Shao
authors.forename: Anle
authors.initials: A
authors.lastname: Wu
authors.forename: Xing Zhong
authors.initials: XZ
authors.lastname: Wu
mesh_terms: D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000378:metabolism
chemical_list: D019938:Cyclin D1; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C530104:CCND1 protein, human; C085126:FOXO1 protein, human; C525067:MIR223, human