title: Efficacy of Fungiflora Y staining for the diagnosis of oral erythematous candidiasis.
abstract: OBJECTIVE
  The purpose of this study was to investigate the efficacy of Fungiflora Y staining (fluorescent stain) for the diagnosis of erythematous candidiasis.
  
  
  METHODS
  This study enrolled 48 patients who were diagnosed with erythematous candidiasis and who underwent fungal culture and microscopic examination of a smear specimen stained with CytoQuick (modification of the Giemsa stain) and Fungiflora Y. Fungiflora Y staining was observed using a portable fluorescent microscope (CyScope(®)). The sensitivity, specificity and positive and negative predictive values were calculated using fungal culture as the gold standard test. Accuracy was calculated, and the difference between the CytoQuick and Fungiflora Y groups was examined using contingency tables and the chi-square test.
  
  
  RESULTS
  The sensitivity and specificity of the CytoQuick stain was 0.51 and 0.91, respectively; the positive predictive value was 0.95, and the negative predictive value was 0.36. The sensitivity and specificity of the Fungiflora Y stain was 0.84 and 1.0, respectively; the positive predictive value was 1.00, and the negative predictive value was 0.65. The accuracy of Fungiflora Y (0.88) was superior to that of CytoQuick (0.60) (p = 0.0052).
  
  
  CONCLUSIONS
  Microscopic examinations of smear specimens using a combination of Fungiflora Y staining and the CyScope(®) portable fluorescent microscope was found to be useful for the diagnosis of oral erythematous candidiasis.
authors.affiliation: Department of Clinical Pathophysiology, Tsurumi University School of Dental Medicine, Tsurumi-ku, Yokohama, Japan.
authors.forename: Mariko R
authors.initials: MR
authors.lastname: Okamoto
authors.forename: Miho
authors.initials: M
authors.lastname: Kamoi
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yamachika
authors.forename: Akihisa
authors.initials: A
authors.lastname: Tsurumoto
authors.forename: Takahiro
authors.initials: T
authors.lastname: Imamura
authors.forename: Ken
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kadomatsu
authors.forename: Ichiro
authors.initials: I
authors.lastname: Saito
authors.forename: Nobuko
authors.initials: N
authors.lastname: Maeda
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nakagawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001399:Azure Stains; D002176:Candida albicans / Q000302:isolation & purification; D041221:Candida glabrata / Q000302:isolation & purification; D041022:Candida tropicalis / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000175:diagnosis*; D004396:Coloring Agents; D003581:Cytodiagnosis / Q000379:methods; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D008875:Middle Aged; D009172:Mycology / Q000379:methods; D009930:Organic Chemicals; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014060:Tongue Diseases / Q000382:microbiology
chemical_list: D001399:Azure Stains; D004396:Coloring Agents; D005456:Fluorescent Dyes; C102655:Fungiflora Y; D009930:Organic Chemicals