title: The effects of lesion of the olfactory epithelium on morphine-induced sensitization and conditioned place preference in mice.
abstract: Animals attain information about their environment through different sense organs. For example, the dominant external resource about the environment for rodents is obtained through olfaction. Many environmental conditions (stress or enriched environment) are known to affect an animal's susceptibility to drug addiction. However, it is not known how external information is integrated and paired with drug stimuli to develop into addictive behavior. Here, we investigated the effects of olfactory epithelium lesions induced with ZnSO4 effusion (ZnE) on morphine-induced sensitization and conditioned place preference in mice. We found that the lesion of the olfactory epithelium attenuated the repeated morphine (40 mg/kg)-induced behavioral sensitization and morphine-induced conditioned place preference (CPP) behaviors, such as hyper-locomotion during morphine (40 mg/kg) conditioned training. Additionally, the expression of FosB-like proteins, transcription factors associated with behavioral alterations, in the nucleus accumbens of the brain was attenuated in morphine administered mice treated by ZnE. Taken together, these results indicated that lesion of the olfactory epithelium lead to a decrease in morphine sensitization and CPP behavior in mice as well as modulate specific molecular markers of neuroadaption to drugs of abuse. These findings also suggest that olfaction plays an important role in the development of addictive behaviors that can be modulated by external actions.
authors.affiliation: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, PR China.
authors.forename: Haichen
authors.initials: H
authors.lastname: Niu
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Zheng
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Rizak
authors.forename: Yaodong
authors.initials: Y
authors.lastname: Fan
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Yuanye
authors.initials: Y
authors.lastname: Ma
authors.forename: Hao
authors.initials: H
authors.lastname: Lei
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003216:Conditioning, Operant / Q000187:drug effects* / Q000502:physiology; D004192:Discrimination, Psychological / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009020:Morphine / Q000494:pharmacology*; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D009294:Narcotics / Q000494:pharmacology*; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism; D009812:Odorants; D009831:Olfactory Mucosa / Q000293:injuries* / Q000502:physiology*; D010775:Photic Stimulation; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D013997:Time Factors; D014110:Touch / Q000502:physiology; D019287:Zinc Sulfate / Q000494:pharmacology
chemical_list: C483819:Fosb protein, mouse; D009294:Narcotics; D016760:Proto-Oncogene Proteins c-fos; D009020:Morphine; D019287:Zinc Sulfate