title: High-capacity and high-intensity DNA microarray spots using oxygen-plasma nanotextured polystyrene slides.
abstract: Commercially available polystyrene (PS) slides were plasma nanotextured (nano-roughened) through treatment in oxygen plasma discharges to create substrates with increased surface area for microarray applications. Conditions of plasma treatment were determined for maximum and uniform oligonucleotide immobilization on these nanotextured PS slides. Oligonucleotides were immobilized onto the surface in the form of biotinylated oligonucleotide/streptavidin conjugates to take advantage of increased protein binding capacity of the substrate. It was found that the amount of oligonucleotides that could be immobilized was increased up to ten times on plasma treated as compared with untreated slides. The sensitivity of detection of labelled hybridized probes was improved by a factor of 20. Optimized nanotextured PS slides were subsequently used to develop a microarray for the detection of three deleterious BRCA1 gene mutations by immobilizing oligonucleotides corresponding to wild and mutant-type sequences. The microarray developed on the nanotextured PS slides provided higher specific hybridization signal and discrimination ratios as compared with flat untreated PS slides.
authors.affiliation: Institute of Microelectronics, NCSR Demokritos, Attiki, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Tsougeni
authors.forename: G
authors.initials: G
authors.lastname: Koukouvinos
authors.forename: P S
authors.initials: PS
authors.lastname: Petrou
authors.forename: A
authors.initials: A
authors.lastname: Tserepi
authors.forename: S E
authors.initials: SE
authors.lastname: Kakabakos
authors.forename: E
authors.initials: E
authors.lastname: Gogolides
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics; D001483:Base Sequence; D019921:Biotinylation; D004252:DNA Mutational Analysis / Q000295:instrumentation*; D006801:Humans; D009154:Mutation; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D010100:Oxygen / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D019313:BRCA1 Protein; D015345:Oligonucleotide Probes; D011137:Polystyrenes; D010100:Oxygen