title: Development of mannosylated liposomes using synthesized N-octadecyl-D-mannopyranosylamine to enhance gastrointestinal permeability for protein delivery.
abstract: The lysozyme (LZ)-entrapped mannosylated liposomes were prepared in this study by the use of N-octadecyl-D-mannopyranosylamine (SAMAN), which had been synthesized in-house and confirmed by characterization with FTIR and NMR. The reactant residues of synthesized SAMAN were found to be less than 1%. The mean sizes, zeta potentials, drug entrapment efficiencies, and loading capacities of all liposomal formulations were in the ranges of 234.7 to 431.0 nm, -10.97 to -25.80 mV, 7.52 to 14.10%, and 1.44 to 2.77%, respectively. The permeability of mannosylated LZ liposomes across Caco-2 cell monolayers was significantly enhanced to about 2.5- and 7-folds over those of conventional liposomes and solution, respectively, which might be due to the role of mannose receptor or mannose-binding protein on the intestinal enterocytes.
authors.affiliation: Department of Manufacturing Pharmacy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhya Road, Bangkok, 10400, Thailand.
authors.forename: Wasu
authors.initials: W
authors.lastname: Witoonsaridsilp
authors.forename: Ornlaksana
authors.initials: O
authors.lastname: Paeratakul
authors.forename: Busaba
authors.initials: B
authors.lastname: Panyarachun
authors.forename: Narong
authors.initials: N
authors.lastname: Sarisuta
mesh_terms: D000606:Amino Sugars / Q000737:chemistry / Q000494:pharmacology*; D001692:Biological Transport; D018938:Caco-2 Cells; D002626:Chemistry, Pharmaceutical; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D007700:Kinetics; D037181:Lectins, C-Type / Q000378:metabolism; D008055:Lipids / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D000090323:Mannose Receptor; D037601:Mannose-Binding Lectin / Q000378:metabolism; D037241:Mannose-Binding Lectins / Q000378:metabolism; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism*; D053758:Nanoparticles; D010316:Particle Size; D010539:Permeability; D011956:Receptors, Cell Surface / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000606:Amino Sugars; D037181:Lectins, C-Type; D008055:Lipids; D000090323:Mannose Receptor; D037601:Mannose-Binding Lectin; D037241:Mannose-Binding Lectins; C575355:N-octadecylmannopyranosylamine; D011956:Receptors, Cell Surface; D009113:Muramidase