title: Molecular cytogenetic characterization and stripe rust response of a trigeneric hybrid involving Triticum, Psathyrostachys, and Thinopyrum.
abstract: Trigeneric hybrids offer opportunities to transfer alien traits into cultivated wheat. In this study, a new trigeneric hybrid involving species of Triticum, Psathyrostachys, and Thinopyrum was synthesized by crossing Triticum aestivum L. (wheat)--Thinopyrum intermedium (Host) Barkworth & D.R. Dewey amphiploid Zhong 3 with wheat--Psathyrostachys huashanica Keng ex Kuo amphiploid PHW-SA. Crossability of the two amphiploids was 19.74%, and the fertility of the hybrid was 16.20%. The mean meiotic configuration of the trigeneric hybrid (2n=56) was 13.06 I+17.24 IIring+3.73 IIrod+0.28 III+0.04 IV. GISH analysis indicated that the trigeneric F1 had seven P. huashanica chromosomes and seven Th. intermedium chromosomes. The mean chromosome numbers of F2, F3, and F4 progenies were 2n=49.24, 2n=48.13, and 2n=46.78, respectively, a gradual decrease. GISH analysis revealed that most F2 and F3 plants had 2–10 Th. intermedium chromosomes and 0–4 P. huashanica chromosomes. In the F4 progenies, 1–7 Th. intermedium chromosomes were labeled, but no P. huashanica chromosomes were detected. It seems that Th. intermedium chromosomes are more likely than P. huashanica chromosomes to be transmitted to the progenies. The stripe rust response of PHW-SA was expressed in the F1 and some F2 and F3 progenies. The trigeneric hybrid could be a useful bridge for transfering P. huashanica and Th. intermedium chromosomes to common wheat.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China.
authors.forename: Houyang
authors.initials: H
authors.lastname: Kang
authors.forename: Jian
authors.initials: J
authors.lastname: Zeng
authors.forename: Quan
authors.initials: Q
authors.lastname: Xie
authors.forename: Shan
authors.initials: S
authors.lastname: Tao
authors.forename: Meiyu
authors.initials: M
authors.lastname: Zhong
authors.forename: Haiqin
authors.initials: H
authors.lastname: Zhang
authors.forename: Xing
authors.initials: X
authors.lastname: Fan
authors.forename: Lina
authors.initials: L
authors.lastname: Sha
authors.forename: Lili
authors.initials: L
authors.lastname: Xu
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D002678:Chimera; D032461:Chromosomes, Plant; D006824:Hybridization, Genetic; D017403:In Situ Hybridization; D059785:Karyotype; D006109:Poaceae / Q000235:genetics*; D014908:Triticum / Q000235:genetics*