title: YrhB is a highly stable small protein with unique chaperone-like activity in Escherichia coli BL21(DE3).
abstract: Escherichia coli YrhB (10.6 kDa) from strain BL21(DE3) that is commonly used for protein overexpression is a stable chaperone-like protein and indispensable for supporting the growth of BL21(DE3) at 48 °C but not defined as conventional heat shock protein (HSP). YrhB effectively prevented heat-induced aggregation of ribonucleotide synthetase (PurK). Without ATP, YrhB alone promoted in vitro refolding of uridine phosphorylase (UDP) and protected thermal denaturation of the refolded UDP. As a cis-acting fusion partner, YrhB also significantly reduced inclusion body formation of nine aggregation-prone heterologous proteins in BL21(DE3). Unlike conventional small HSPs, YrhB remained monomer under heat shock condition.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Anam-Dong 5-1, Sungbuk-Gu, Seoul 136-713, Republic of Korea.
authors.forename: Keum-Young
authors.initials: KY
authors.lastname: Ahn
authors.forename: Jin-Seung
authors.initials: JS
authors.lastname: Park
authors.forename: Kyung-Yeon
authors.initials: KY
authors.lastname: Han
authors.forename: Jong-Am
authors.initials: JA
authors.lastname: Song
authors.forename: Jeewon
authors.initials: J
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000737:chemistry / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018869:Heat-Shock Response; D006358:Hot Temperature; D002479:Inclusion Bodies; D007700:Kinetics; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D009154:Mutation; D011401:Promoter Regions, Genetic; D011489:Protein Denaturation; D058849:Protein Refolding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism; D014543:Uridine Phosphorylase / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D012808:Sigma Factor; C572416:YrhB protein, E coli; D014543:Uridine Phosphorylase; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D002262:Carboxy-Lyases; C075015:purK protein, E coli