title: [Study on the molecular-epidemiological characteristics of HIV-1 in Shenzhen, 1992-2008].
abstract: OBJECTIVE
  To investigate the epidemiological characteristics of HIV-1 subtype in Shenzhen from 1992 to 2008.
  
  
  METHODS
  489 HIV-1 positive plasma samples were collected from 1992 to 2008 in Shenzhen. HIV-1 env genes were amplified by nested-PCR from RNA. Phylogenetic analysis was performed on data regarding the nucleotide sequence.
  
  
  RESULTS
  A total of 464 sequences were amplified and genotyped. Data from this study revealed that CRF01_AE was a predominant HIV-1 subtype in Shenzhen (64.4%, 299/464), followed by subtypes CRF_BC (17.5%, 81/464), B' (14.7%, 68/464) and B (2.4%, 11/464). Subtype C (0.4%, 2/464), A1 (0.2%, 1/464), CRF02_AG (0.2%, 1/464) and CRF06_cpx (0.2%, 1/464) were also prevalent in Shenzhen. CRF01_AE and CRF_BC were predominant among heterosexuals, homosexuals and injection drug users, while B' was predominant among blood donors. Results from phylogenetic tree analysis showed that some of the HIV-1 clusters had been defined in CRF01_AE strains at different time or groups with different transmission routes. Cross-infections were also seen.
  
  
  CONCLUSIONS
  CRF01_AE was the predominant HIV-1 subtype in Shenzhen while CRF_BC, B, B', C, A1, CRF02_AG and a small amount of CRF06_cpx or recombinant subtypes were prevalent in this city. Different subtypes showed great variation in the process of epidemics.
authors.affiliation: Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China.
authors.forename: Guang-lu
authors.initials: GL
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Juan-juan
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Tie-jian
authors.initials: TJ
authors.lastname: Feng
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Fu-chang
authors.initials: FC
authors.lastname: Hong
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
mesh_terms: D002681:China / Q000453:epidemiology; D005260:Female; D015497:HIV-1 / Q000145:classification / Q000235:genetics; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology