title: SUMOylation of Blimp-1 is critical for plasma cell differentiation.
abstract: Transcriptional repressor B lymphocyte-induced maturation protein-1 (Blimp-1) is a master regulator of plasma cell differentiation. Here we show that Blimp-1 is covalently modified by SUMO1 at lysine 816, a modification mediated by SUMO E3 ligase PIAS1. Mutation of Blimp-1 lysine 816 reduces transcriptional repression--correlating with a reduced interaction with a histone deacetylase, HDAC2--and impairs differentiation of antibody-secreting cells. Thus, the SUMO pathway critically regulates Blimp-1 function during plasma cell differentiation.
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Hsia-Yuan
authors.initials: HY
authors.lastname: Ying
authors.forename: Shin-Tang
authors.initials: ST
authors.lastname: Su
authors.forename: Pang-Hung
authors.initials: PH
authors.lastname: Hsu
authors.forename: Che-Chang
authors.initials: CC
authors.lastname: Chang
authors.forename: I-Ying
authors.initials: IY
authors.lastname: Lin
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Tseng
authors.forename: Ming-Daw
authors.initials: MD
authors.lastname: Tsai
authors.forename: Hsiu-Ming
authors.initials: HM
authors.lastname: Shih
authors.forename: Kuo-I
authors.initials: KI
authors.lastname: Lin
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D005786:Gene Expression Regulation; D056464:Histone Deacetylase 2 / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D010950:Plasma Cells / Q000166:cytology* / Q000378:metabolism*; D000074462:Positive Regulatory Domain I-Binding Factor 1; D050828:Protein Inhibitors of Activated STAT / Q000378:metabolism; D025842:SUMO-1 Protein / Q000378:metabolism*; D058207:Sumoylation; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C494211:Pias1 protein, mouse; C513258:Prdm1 protein, mouse; D050828:Protein Inhibitors of Activated STAT; D025842:SUMO-1 Protein; D014157:Transcription Factors; D000074462:Positive Regulatory Domain I-Binding Factor 1; C533424:Hdac2 protein, mouse; D056464:Histone Deacetylase 2; D008239:Lysine