title: Nerve-targeted desensitizing toothpastes occlude dentin tubules and induce mineral precipitation.
abstract: OBJECTIVE
  To examine the laboratory dentin tubules occlusion and mineral precipitation capability of two potassium salts-containing desensitizing toothpastes.
  
  
  METHODS
  40 dentin disks were obtained and divided into four groups, including artificial saliva (AS), distilled water (DW), Sensodyne Freshmint (SF) and Colgate Sensitive (CS). Dentin permeability measurement was performed after EDTA etching, initial brush, 3-day brush, 7-day brush and citric acid challenge, respectively. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) was used to monitor the variation of mineral content before and after treatments. The ultra-morphology of dentin surface was examined using scanning electron microscopy (SEM) to evaluate tubule occlusion.
  
  
  RESULTS
  The two potassium salts-containing desensitizing toothpastes significantly reduced dentin permeability to less than 40% after 7-day treatment (P<0.05). The ATR-FTIR analysis showed increase of the intensity of the phosphate peak after a 7-day brushing period using both of the tested toothpastes. However, the dentin permeability significantly increased and the intensity of phosphate peak decreased after acid etching for all groups. The SEM examination revealed partially occluded dentin tubules after toothpaste treatments, but after the acid challenge the tubules were opened again.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, PR China.
authors.forename: Zhejun
authors.initials: Z
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sauro
authors.forename: Yining
authors.initials: Y
authors.lastname: Wang
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Xing
authors.forename: Shanshan
authors.initials: S
authors.lastname: Liang
authors.forename: Yue
authors.initials: Y
authors.lastname: Sa
authors.forename: Chengfei
authors.initials: C
authors.lastname: Zhang
authors.forename: Ya
authors.initials: Y
authors.lastname: Shen
authors.forename: Markus
authors.initials: M
authors.lastname: Haapasalo
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000328:Adult; D011232:Chemical Precipitation; D019343:Citric Acid / Q000494:pharmacology; D003804:Dentin / Q000187:drug effects* / Q000294:innervation / Q000648:ultrastructure; D057133:Dentin Desensitizing Agents / Q000494:pharmacology*; D003806:Dentin Permeability / Q000187:drug effects; D004492:Edetic Acid / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000737:chemistry; D009411:Nerve Endings / Q000187:drug effects; D009431:Neural Conduction / Q000187:drug effects; D009566:Nitrates / Q000494:pharmacology; D019357:Potassium Citrate / Q000494:pharmacology; D017680:Potassium Compounds / Q000494:pharmacology*; D012464:Saliva, Artificial / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014099:Toothbrushing; D014100:Toothpastes / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
chemical_list: D057133:Dentin Desensitizing Agents; D008903:Minerals; D009566:Nitrates; D017680:Potassium Compounds; D012464:Saliva, Artificial; D014100:Toothpastes; D014867:Water; D019343:Citric Acid; D004492:Edetic Acid; D019357:Potassium Citrate; C023844:potassium nitrate