title: Rapid detection of intestinal carriage of Klebsiella pneumoniae producing KPC carbapenemase during an outbreak.
abstract: Two different approaches are described for rapid detection of intestinal carriage of Klebsiella pneumoniae producing KPC-type carbapenemase (KPC-KP), based on PCR amplification of DNA extracts from rectal swabs (K-PCR), and on direct plating of rectal swabs on to MacConkey agar with a meropenem disc and a meropenem plus 3-aminophenylboronic acid disc (direct KPC screening test, DKST). K-PCR and DKST were tested with a total of 101 samples from 65 patients, during an outbreak. Although less sensitive, DKST could detect high-level carriage, which appears to be common among infected and colonised patients, while being very cheap and easy to perform, and requiring only basic facilities.
authors.affiliation: Department of Biotechnologies, Section of Microbiology, University of Siena, Siena, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Giani
authors.forename: C
authors.initials: C
authors.lastname: Tascini
authors.forename: F
authors.initials: F
authors.lastname: Arena
authors.forename: I
authors.initials: I
authors.lastname: Ciullo
authors.forename: V
authors.initials: V
authors.lastname: Conte
authors.forename: A
authors.initials: A
authors.lastname: Leonildi
authors.forename: F
authors.initials: F
authors.lastname: Menichetti
authors.forename: G M
authors.initials: GM
authors.lastname: Rossolini
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002353:Carrier State / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D004196:Disease Outbreaks; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007710:Klebsiella Infections / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests / Q000191:economics / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013997:Time Factors; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase