title: The ribosome modulates the structural dynamics of the conserved GTPase HflX and triggers tight nucleotide binding.
abstract: The universally conserved GTPase HflX is a putative translation factor whose GTPase activity is stimulated by the 70S ribosome as well as the 50S but not the 30S ribosomal subunit. However, the details and mechanisms governing this interaction are only poorly understood. In an effort to further elucidate the functional mechanism of HflX, we examined its interaction with the 70S ribosome, the two ribosomal subunits (50S and 30S), as well as its ability to interact with guanine nucleotides in the respective ribosomal complexes using a highly purified in vitro system. Binding studies reported here demonstrate that HflX not only interacts with 50S and 70S particles, but also with the 30S subunit, independent of the nucleotide-bound state. A detailed pre-steady-state kinetic analysis of HflX interacting with a non-hydrolyzable analog of mant-GTP, coupled with an enzymatic probing assay utilizing limited trypsinolysis, reveal that HflX·GTP exists in a structurally distinct 50S- and 70S-bound form that stabilizes GTP binding up to 70 000-fold and that may represent the "GTPase-activated" state. This activation is likely required for efficient GTP-hydrolysis, and may be similar to that observed in elongation factor G. Results reported here address the surprising low affinity of free HflX for GTP and suggest that cellular HflX will mainly exist in the HflX·GTP·ribosome-bound form. A minimal model for the functional cycle of HflX is proposed.
authors.affiliation: Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge, Alberta T1K 3M4, Canada.
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Fischer
authors.forename: Mackenzie L
authors.initials: ML
authors.lastname: Coatham
authors.forename: Shey Eagle
authors.initials: SE
authors.lastname: Bear
authors.forename: Harland E
authors.initials: HE
authors.lastname: Brandon
authors.forename: Evelina I
authors.initials: EI
authors.lastname: De Laurentiis
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Shields
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Wieden
mesh_terms: D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000737:chemistry*; D019204:GTP-Binding Proteins / Q000737:chemistry*; D006150:Guanine Nucleotides / Q000737:chemistry* / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D020653:Peptide Elongation Factor G / Q000737:chemistry; D011487:Protein Conformation; D054681:Ribosome Subunits, Large, Bacterial / Q000737:chemistry / Q000378:metabolism; D054680:Ribosome Subunits, Small, Bacterial / Q000737:chemistry / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D006150:Guanine Nucleotides; D020653:Peptide Elongation Factor G; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins; C535071:HflX protein, E coli