title: Unique short tandem repeat nucleotide sequences in Entamoeba histolytica isolates from China.
abstract: A few PCR-based DNA typing methods using repetitive elements contained within both protein-coding genes and noncoding DNAs have been reported for Entamoeba histolytica over the years. The serine-rich E. histolytica protein and tRNA-linked short tandem repeats (STRs) are most commonly used to investigate the relationship between parasite genotype and E. histolytica infection outcome. Many E. histolytica infections in China have been reported; however, little genome information has been provided. In the current paper, five Chinese E. histolytica samples were reported: three amoebic liver abscess cases, one combined case and one asymptomatic case. Our study is the first to report on the DNA typing information of E. histolytica in China. We included two city, one imported, and two country cases. Sequence analysis of serine-rich protein genes confirmed the presence of seven sequence types in five isolates. The STRs amplified from the samples revealed five STR variations in the A-L, four in the N-K2, and R-R loci, three in D-A, S(TGA)-D and S-Q loci. Two country patients were found to have a different outcome of infection with the same genotypes of E. histolytica, whereas in a city case, one E. histolytica strain had led to different outcome of the infection in one patient. Analyses of the results suggest that more genome information of E. histolytica strains from China through accurate methods is needed to interpret how the parasite genome plays a role in determining the outcome of E. histolytica infections.
authors.affiliation: Department of Medical Microbiology and Parasitology, Shanghai Medical College of Fudan University, Shanghai, 200032, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Feng
authors.forename: Junlong
authors.initials: J
authors.lastname: Cai
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Fu
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Min
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tachibana
authors.forename: Xunjia
authors.initials: X
authors.lastname: Cheng
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D004748:Entamoeba histolytica / Q000235:genetics*; D006801:Humans; D008100:Liver Abscess / Q000453:epidemiology / Q000469:parasitology; D008101:Liver Abscess, Amebic / Q000453:epidemiology / Q000469:parasitology*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods