title: Oligonucleotide-stabilized fluorescent silver nanoclusters for turn-on detection of melamine.
abstract: Fluorescence nanoclusters have been used for the determination of melamine for the first time. The method is based on the fluorescence turn-on of oligonucleotide-stabilized silver nanoclusters (DNA-Ag NCs) by melamine. The enhancement factors (I-I(0))/I(0) increase linearly with melamine concentrations over the range 5.0×10(-8)-7.0×10(-6) M (R(2)=0.998). The detection limit is 1.0×10(-8) M, which is approximately 2000 times lower than the US Food and Drug Administration estimated melamine safety limit of 20.0 μM. Furthermore, the milk samples spiked with melamine are analyzed with excellent recoveries.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Han
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zhu
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Lianzhe
authors.initials: L
authors.lastname: Hu
authors.forename: Saima
authors.initials: S
authors.lastname: Parveen
authors.forename: Guobao
authors.initials: G
authors.lastname: Xu
mesh_terms: D000818:Animals; D002417:Cattle; D005456:Fluorescent Dyes / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D008892:Milk / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D014227:Triazines / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides; D014227:Triazines; D012834:Silver; C011907:melamine