title: Dissecting Arabidopsis Gβ signal transduction on the protein surface.
abstract: The heterotrimeric G-protein complex provides signal amplification and target specificity. The Arabidopsis (Arabidopsis thaliana) Gβ-subunit of this complex (AGB1) interacts with and modulates the activity of target cytoplasmic proteins. This specificity resides in the structure of the interface between AGB1 and its targets. Important surface residues of AGB1, which were deduced from a comparative evolutionary approach, were mutated to dissect AGB1-dependent physiological functions. Analysis of the capacity of these mutants to complement well-established phenotypes of Gβ-null mutants revealed AGB1 residues critical for specific AGB1-mediated biological processes, including growth architecture, pathogen resistance, stomata-mediated leaf-air gas exchange, and possibly photosynthesis. These findings provide promising new avenues to direct the finely tuned engineering of crop yield and traits.
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
authors.forename: Kun
authors.initials: K
authors.lastname: Jiang
authors.forename: Arwen
authors.initials: A
authors.lastname: Frick-Cheng
authors.forename: Yuri
authors.initials: Y
authors.lastname: Trusov
authors.forename: Magdalena
authors.initials: M
authors.lastname: Delgado-Cerezo
authors.forename: David M
authors.initials: DM
authors.lastname: Rosenthal
authors.forename: Justine
authors.initials: J
authors.lastname: Lorek
authors.forename: Ralph
authors.initials: R
authors.lastname: Panstruga
authors.forename: Fitzgerald L
authors.initials: FL
authors.lastname: Booker
authors.forename: José Ramón
authors.initials: JR
authors.lastname: Botella
authors.forename: Antonio
authors.initials: A
authors.lastname: Molina
authors.forename: Donald R
authors.initials: DR
authors.lastname: Ort
authors.forename: Alan M
authors.initials: AM
authors.lastname: Jones
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000383:Agriculture; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D005408:Flagellin / Q000494:pharmacology; D044387:GTP-Binding Protein beta Subunits / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D030821:Plants, Genetically Modified; D017510:Protein Folding / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013499:Surface Properties / Q000187:drug effects
chemical_list: C089590:AGB1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D044387:GTP-Binding Protein beta Subunits; D050505:Mutant Proteins; D017382:Reactive Oxygen Species; D005408:Flagellin; D000040:Abscisic Acid; D005947:Glucose