title: Sphingobium czechense sp. nov., isolated from a hexachlorocyclohexane dump site.
abstract: A yellow-pigmented bacterial strain, designated LL01(T), was isolated from hexachlorocyclohexane (HCH)-contaminated soil at Spolana Neratovice, a former Czech producer of lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL01(T) occupied a distinct phylogenetic position in the Sphingobium cluster, showing highest similarity to Sphingobium rhizovicinum CC-FH12-1(T) (98.5 %). The DNA G+C content of strain LL01(T) was 66.1 mol%. The predominant respiratory pigment was ubiquinone Q-10. The polar lipid profile of strain LL01(T) also corresponded to those reported for other Sphingobium species (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipids), supporting its identification as a member of the genus Sphingobium. Spermidine was the major polyamine observed. The results obtained from DNA-DNA hybridization and biochemical and physiological tests clearly distinguished strain LL01(T) from closely related species of the genus Sphingobium. Therefore, strain LL01(T) represents a novel species of the genus Sphingobium, for which the name Sphingobium czechense sp. nov. is proposed (type strain LL01(T) = CCM 7979(T) = DSM 25410(T)).
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi - 110007, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Niharika
authors.affiliation: International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Moskalikova
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi - 110007, India.
authors.forename: Jasvinder
authors.initials: J
authors.lastname: Kaur
authors.affiliation: IMTECH - Institute of Microbial Technology, Sector-39A, Chandigarh - 160036, India.
authors.forename: Fazlurrahman
authors.initials: F
authors.lastname: Khan
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Sedlackova
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Hampl
authors.affiliation: Loschmidt Laboratories and Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, 628 00 Brno, Czech Republic.|International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91 Brno, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Damborsky
authors.affiliation: Loschmidt Laboratories and Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, 628 00 Brno, Czech Republic.
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Prokop
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi - 110007, India.
authors.forename: Rup
authors.initials: R
authors.lastname: Lal
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D001556:Hexachlorocyclohexane; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D013095:Spermidine / Q000032:analysis; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D014451:Ubiquinone; D001556:Hexachlorocyclohexane; C025204:Ubiquinone Q2; D013095:Spermidine