title: Plasticity and banking potential of cultured adipose tissue derived mesenchymal stem cells.
abstract: The present day research on stem cells is yet not filled to the gunwales. The correlation of stem cell technology with tissue repair still has a long way to go. Since Embryonic stem cells are a kind of thorn inside when it comes to therapeutics, there emerged few potent contemporary sources of stem cells. Though bone marrow proves to be the pioneer among these, they lose themselves to adipose tissue in various aspects. The major shortcoming of bone marrow lies in lieu of its loss in potency with age. Adipose tissue puts up a tough competition among leading edge stem cell sources like cord blood and cord matrix. Adipose tissue wins over its counterparts in that it possesses astounding proliferation potency in vitro and holds a prominent stand in showcasing in vivo tissue repair efficacy. In spite of its precedence, the whole enchilada of adipose derived stem cells is still in its salad days. In our work we aim at excogitating the Mesenchymal stem cell population present in cultured adipose derived stem cells, in a wide perspective. Furthermore, the coalition of cell adhesion molecules with the proliferation potency of MSC and analysis of growth curve of ADSC was also paid accolade. The presence of robust MSC with immense differentiation and transdifferentiation potency was endorsed by lucrative differentiation of P3 cells into mesodermal and neuronal lineages. Additionally, mesenchymal stem cells exhibiting coherent expression of surface markers at P3 in all samples can be cryopreserved for therapeutic applications.
authors.affiliation: Loyola College, Nungambakkam, Chennai, Tamilnadu, India. sekarbiochem@yahoo.co.in
authors.forename: M
authors.initials: M
authors.lastname: Dhanasekaran
authors.forename: S
authors.initials: S
authors.lastname: Indumathi
authors.forename: R
authors.initials: R
authors.lastname: Poojitha
authors.forename: A
authors.initials: A
authors.lastname: Kanmani
authors.forename: J S
authors.initials: JS
authors.lastname: Rajkumar
authors.forename: D
authors.initials: D
authors.lastname: Sudarsanam
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D000328:Adult; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D054338:Cell Transdifferentiation / Q000502:physiology*; D002478:Cells, Cultured; D015925:Cryopreservation; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D008875:Middle Aged; D014015:Tissue Banks
chemical_list: D015815:Cell Adhesion Molecules; D008565:Membrane Proteins