title: [Effect of mycophenolate on expression of MCP-1 and fibronectin in human mesangial cells induced by high glucose].
abstract: OBJECTIVE
  To investigate the effect of high glucose and mycophenolate (MMF) on the expression of MCP-1 in human mesangial cells (HMCs) and fibronectin (FN).
  
  
  METHODS
  The HMCs were divided randomly into five groups:  control group (5 mmol/L glucose),  high glucose group (30 mmol/L glucose),  mannitol group (5 mmol/L glucose and 25 mmol/L mannitol),  high glucose+MMF-10 group (30 mmol/L glucose plus 10 μg/mL  mycophenolate) and high glucose+MMF-100 group (30 mmol/L glucose plus 100 μg/mL mycophenolate). We detected the levels of MCP-1 and fibronectin in each group at 24 h,  48 h and 72 h, respectively. The expression levels of the MCP-1 mRNA were detected by RT-PCR, and the protein expression of MCP-1 and fibronectin was measured by ELISA.
  
  
  RESULTS
  Compared with the control group,  the levels of the MCP-1 and FN in high glucose group were significantly increased  with the expression peak at 48 h (P<0.01). The MMF with different concentration could inhibit the expression of MCP-1 and FN in time- and dose-dependent manner (P<0.05).
  
  
  CONCLUSIONS
  Mycophenolate could inhibit the expressions of MCP-1 and FN in human mesangial cells and it might be expected to delay the development and progression of glomerular sclerosis and interstitial fibrosis.
authors.affiliation: Department of Geriatrics, the First Affiliated Hospital, China Medical University, Shenyang, China.
authors.forename: Fen-qin
authors.initials: FQ
authors.lastname: Chen
authors.forename: Qiu-yue
authors.initials: QY
authors.lastname: Wang
authors.forename: Guo-zhu
authors.initials: GZ
authors.lastname: Wei
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Ma
authors.forename: Dong-wei
authors.initials: DW
authors.lastname: Ma
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Deng
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Sun
mesh_terms: D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000032:analysis* / Q000235:genetics; D005353:Fibronectins / Q000032:analysis*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D050527:Mesangial Cells / Q000737:chemistry / Q000187:drug effects*; D009173:Mycophenolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D005353:Fibronectins; D012333:RNA, Messenger; D009173:Mycophenolic Acid; D005947:Glucose