title: Zebrafish p53 protein enhances the translation of its own mRNA in response to UV irradiation and CPT treatment.
abstract: p53 protein is an important regulatory factor involved in cell growth and development. In our previous study, we demonstrated that recombined zebrafish p53 protein could specifically bind to its own mRNA in vitro. To determine if a similar interaction exists in zebrafish and if this interaction affects zebrafish development, in the present study, we investigated the interaction of p53 protein and its mRNA in zebrafish embryos. Our results revealed that expressed zebrafish p53 protein could bind with its own mRNA in zebrafish embryos. Furthermore, the endogenous activated or ectopically expressed p53 protein could enhance the relative activity of Renilla luciferase fused with p53 3'UTR in response to UV irradiation and CPT treatment, and retarded development of zebrafish embryos was observed.
authors.affiliation: Key Laboratory for Rare Diseases of Shandong Province, Institute of Basic Medicine, Shandong Academy of Medical Science, Jinan 250062, China.
authors.forename: Xiangzhong
authors.initials: X
authors.lastname: Zhao
authors.forename: Ning
authors.initials: N
authors.lastname: Wu
authors.forename: Lili
authors.initials: L
authors.lastname: Ding
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Haizhou
authors.initials: H
authors.lastname: Liu
authors.forename: Xiukun
authors.initials: X
authors.lastname: Lin
mesh_terms: D000818:Animals; D002166:Camptothecin / Q000494:pharmacology*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism; D014466:Ultraviolet Rays; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D029961:Zebrafish Proteins; D002166:Camptothecin