title: Inactivation of Dicer1 has a severe cumulative impact on the formation of mature germ cells in mouse testes.
abstract: Dicer1, an RNase III endonuclease, is indispensable for the maturation of miRNA and siRNA, which control gene expression through the RNAi pathway. The diverse functions of miRNA involving multiple developmental processes have been elucidated, but the role of Dicer1 in spermatogenesis is just beginning to be revealed. Mice lacking Dicer1 were reported to be embryonic lethal at E7.5. In the present study, mice with a Dicer1 conditional allele were crossed with Vasa-cre transgenic mice to delete Dicer1 as early as the prospermatogonia stage (at E15). At P40, seminiferous tubules of Dicer1 deficient mice showed several aberrant phenotypes. A large number of apoptotic germ cells were detected by the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) assay, but several events in meiosis of spermatocytes appeared unaffected. The mutant mice were found to be sterile, likely due to the extensive decrease in number and morphological abnormalities of mature sperm in the epididymis, which, together with the numerous haploid cells in the testis, indicated a severely affected transition from round to functional elongated spermatozoa. Additionally, we found milder phenotypes when Dicer1 was inactivated in later stages of spermatogenesis in Stra8-cre and Pgk2-cre transgenic mice. In conclusion, our findings suggest that the loss of Dicer1 has a continuous and cumulative effect on the process of spermatogenesis and blocks the germ cells in the stage of round spermatids to a large extent, ultimately leading to the generation of abnormal sperm.
authors.affiliation: Key Laboratory of National Reproductive Medicine, Department of Cell Biology and Medical Genetics, Nanjing Medical University, Nanjing, Jiangsu 210029, China. liunightelf@yahoo.cn
authors.forename: Dekang
authors.initials: D
authors.lastname: Liu
authors.forename: Liangyun
authors.initials: L
authors.lastname: Li
authors.forename: Heling
authors.initials: H
authors.lastname: Fu
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Li
authors.forename: Jianmin
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D053487:DEAD-box RNA Helicases / Q000235:genetics*; D005260:Female; D005298:Fertility / Q000235:genetics; D008297:Male; D008540:Meiosis / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D043244:Ribonuclease III / Q000235:genetics*; D013090:Spermatocytes / Q000166:cytology / Q000201:enzymology; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000166:cytology* / Q000201:enzymology; D013737:Testis / Q000166:cytology* / Q000201:enzymology
chemical_list: C509781:Dicer1 protein, mouse; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases