title: Deep and clear optical imaging of thick inhomogeneous samples.
abstract: Inhomogeneity in thick biological specimens results in poor imaging by light microscopy, which deteriorates as the focal plane moves deeper into the specimen. Here, we have combined selective plane illumination microscopy (SPIM) with wavefront sensor adaptive optics (wao). Our waoSPIM is based on a direct wavefront measure using a Hartmann-Shack wavefront sensor and fluorescent beads as point source emitters. We demonstrate the use of this waoSPIM method to correct distortions in three-dimensional biological imaging and to improve the quality of images from deep within thick inhomogeneous samples.
authors.affiliation: University of Toulouse, ITAV-UMS3039, Toulouse, France.
authors.forename: Raphael
authors.initials: R
authors.lastname: Jorand
authors.forename: Gwénaële
authors.initials: G
authors.lastname: Le Corre
authors.forename: Jordi
authors.initials: J
authors.lastname: Andilla
authors.forename: Amina
authors.initials: A
authors.lastname: Maandhui
authors.forename: Céline
authors.initials: C
authors.lastname: Frongia
authors.forename: Valérie
authors.initials: V
authors.lastname: Lobjois
authors.forename: Bernard
authors.initials: B
authors.lastname: Ducommun
authors.forename: Corinne
authors.initials: C
authors.lastname: Lorenzo
mesh_terms: D005453:Fluorescence; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008027:Light; D008029:Lighting; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D055096:Optical Devices; D055095:Optics and Photonics / Q000295:instrumentation / Q000379:methods*