title: Electrospun nanostructured chitosan-poly(vinyl alcohol) scaffolds: a biomimetic extracellular matrix as dermal substitute.
abstract: Electrospinning is a versatile technique to make biomimetic and nanostructured scaffolds for skin tissue engineering. In this study we have electrospun and characterized chitosan (C)-poly(vinyl alcohol) (PVA) blend nanofibers as dermal substitutes and compared with 2D C-PVA films. The in vitro characterization of the C-PVA nanofibers and 2D films were evaluated using mouse 3T3 fibroblast cells and our results demonstrated that the cells adhered and proliferated on the surface of C-PVA nanofibers. In our animal studies, the implantation of C-PVA nanofibers along with topical administration of growth factor R-Spondin 1 on full thickness wounds created on rats showed 98.6% wound closure after two weeks post-surgery. The catalase and superoxide dismutase activity of the healing tissue was significantly higher in the groups treated with topical administration of growth factor and C-PVA nanofibers (p < 0.05). Thus these C-PVA nanofibers along with novel growth factor are promising new biomaterials that could be used as dermal substitutes for accelerated wound healing.
authors.affiliation: Centre for Nanotechnology and Advanced Biomaterials, SASTRA University, Thanjavur, Tamil Nadu, India.
authors.forename: Dhakshinamoorthy
authors.initials: D
authors.lastname: Sundaramurthi
authors.forename: Kirthanashri Srinivasan
authors.initials: KS
authors.lastname: Vasanthan
authors.forename: Purushothaman
authors.initials: P
authors.lastname: Kuppan
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Krishnan
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethuraman
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D032701:Biomimetics; D002448:Cell Adhesion; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D017768:Skin, Artificial; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013314:Stress, Mechanical; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D011142:Polyvinyl Alcohol; D048271:Chitosan