title: Live imaging of mitosomes and hydrogenosomes by HaloTag technology.
abstract: Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes.
authors.affiliation: Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Martincová
authors.forename: Luboš
authors.initials: L
authors.lastname: Voleman
authors.forename: Vladimíra
authors.initials: V
authors.lastname: Najdrová
authors.forename: Maximiliano
authors.initials: M
authors.lastname: De Napoli
authors.forename: Shiri
authors.initials: S
authors.lastname: Eshar
authors.forename: Melisa
authors.initials: M
authors.lastname: Gualdron
authors.forename: Christine S
authors.initials: CS
authors.lastname: Hopp
authors.forename: David E
authors.initials: DE
authors.lastname: Sanin
authors.forename: Dumizulu L
authors.initials: DL
authors.lastname: Tembo
authors.forename: Daria
authors.initials: D
authors.lastname: Van Tyne
authors.forename: Dawn
authors.initials: D
authors.lastname: Walker
authors.forename: Michaela
authors.initials: M
authors.lastname: Marcinčiková
authors.forename: Jan
authors.initials: J
authors.lastname: Tachezy
authors.forename: Pavel
authors.initials: P
authors.lastname: Doležal
mesh_terms: D000693:Anaerobiosis; D002470:Cell Survival; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D016829:Giardia lamblia / Q000166:cytology / Q000235:genetics; D006867:Hydrolases / Q000235:genetics; D008024:Ligands; D008928:Mitochondria / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods*; D015388:Organelles / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014246:Trichomonas vaginalis / Q000166:cytology / Q000235:genetics
chemical_list: D008024:Ligands; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D006867:Hydrolases; C046137:haloalkane dehalogenase