title: RNA-dependent regulation of the cell wall stress response.
abstract: Stress response requires the precise modulation of gene expression in response to changes in growth conditions. This report demonstrates that selective nuclear mRNA degradation is required for both the cell wall stress response and the regulation of the cell wall integrity checkpoint. More specifically, the deletion of the yeast nuclear dsRNA-specific ribonuclease III (Rnt1p) increased the expression of the mRNAs associated with both the morphogenesis checkpoint and the cell wall integrity pathway, leading to an attenuation of the stress response. The over-expression of selected Rnt1p substrates, including the stress associated morphogenesis protein kinase Hsl1p, in wild-type cells mimicked the effect of RNT1 deletion on cell wall integrity, and their mRNAs were directly cleaved by the recombinant enzyme in vitro. The data supports a model for gene regulation in which nuclear mRNA degradation optimizes the cell response to stress and links it to the cell cycle.
authors.affiliation: RNA Group, Département de microbiologie et d'infectiologie, Faculté de médecine et des sciences de santé, Université de Sherbrooke, Pavillon de recherche appliquée sur cancer, Sherbrooke, Québec, J1E 4K8, Canada.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Catala
authors.forename: Leyla
authors.initials: L
authors.lastname: Aksouh
authors.forename: Sherif
authors.initials: S
authors.lastname: Abou Elela
mesh_terms: D059447:Cell Cycle Checkpoints / Q000235:genetics*; D002473:Cell Wall / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C106494:HSL1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; C468367:RNT1 protein, S cerevisiae; D043244:Ribonuclease III