title: Reprocessing of dental instruments in washer-disinfectors: does a representative test soil exist in dentistry?
abstract: BACKGROUND
  Reprocessing of medical devices, being classified as semi-critical B is recommended to be performed in a washer-disinfector. In order to estimate, whether the expected contaminants of the various medical disciplines can be effectively removed by this washer-disinfector, different so called "test soils" have been proposed to be tested as a marker of cleaning efficacy of the disinfector. Todays described test soils are optimised for the testing of contaminations occurring in surgical procedures, but not for dental procedures.
  
  
  METHODS
  In this study the test soils being proposed in the EN 15883-5 (e.g. KMNE soil, recipe by Koller and coagulated sheep's blood) were compared with 8 reference substances used in the conservative-prosthetic dental practice. The success of the cleaning efficacy in the washer-disinfector was checked visually and by determining the residual protein concentration on the contaminated instruments after the cleaning procedure.
  
  
  RESULTS
  It could be shown that in contrast to the proposed test soils of the EN 15883-5, the used reference substances of the dental practice could not be removed by the washer-disinfector. Removal of these reference substances was only possible after manual or ultrasonic cleaning.
  
  
  CONCLUSIONS
  Since blood plays a subordinate role as a contaminant of instruments during conservative-prosthetic dental treatments, testing of the cleaning efficacy of the washer-disinfector with test soils according to the proposals of the EN 15883-5 is not representative in this discipline of dentistry. Most of the materials used in dental practice can only be removed manually or with the help of the ultrasound bath.
authors.affiliation: Bernhard Gottlieb University Clinic of Dentistry, Central Research Unit, Medical University of Vienna, Vienna, Austria.
authors.forename: Alexander
authors.initials: A
authors.lastname: Franz
authors.forename: Margit
authors.initials: M
authors.lastname: Bristela
authors.forename: Fritz
authors.initials: F
authors.lastname: Stauffer