title: Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor.
abstract: Transplantation of bioengineered elastic cartilage is considered to be a promising approach for patients with craniofacial defects. We have previously shown that human ear perichondrium harbors a population of cartilage progenitor cells (CPCs). The aim of this study was to examine the use of a rotating wall vessel (RWV) bioreactor for CPCs to engineer 3-D elastic cartilage in vitro. Human CPCs isolated from ear perichondrium were expanded and differentiated into chondrocytes under 2-D culture conditions. Fully differentiated CPCs were seeded into recently developed pC-HAp/ChS (porous material consisted of collagen, hydroxyapatite, and chondroitinsulfate) scaffolds and 3-D cultivated utilizing a RWV bioreactor. 3-D engineered constructs appeared shiny with a yellowish, cartilage-like morphology. The shape of the molded scaffold was maintained after RWV cultivation. Hematoxylin and eosin staining showed engraftment of CPCs inside pC-HAp/ChS. Alcian blue and Elastica Van Gieson staining showed of proteoglycan and elastic fibers, which are unique extracellular matrices of elastic cartilage. Thus, human CPCs formed elastic cartilage-like tissue after 3-D cultivation in a RWV bioreactor. These techniques may assist future efforts to reconstruct complicate structures composed of elastic cartilage in vitro.
authors.affiliation: Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Kanazawa-ku, Yokohama, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takebe
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Kan
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Yabuki
authors.forename: M
authors.initials: M
authors.lastname: Mizuno
authors.forename: T
authors.initials: T
authors.lastname: Adegawa
authors.forename: T
authors.initials: T
authors.lastname: Yoshioka
authors.forename: J
authors.initials: J
authors.lastname: Tanaka
authors.forename: J
authors.initials: J
authors.lastname: Maegawa
authors.forename: H
authors.initials: H
authors.lastname: Taniguchi
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D019149:Bioreactors; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism* / Q000473:pathology; D020219:Chondrogenesis; D002809:Chondroitin Sulfates / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D000013:Congenital Abnormalities / Q000473:pathology; D065817:Congenital Microtia; D017886:Durapatite / Q000378:metabolism; D004423:Ear / Q000002:abnormalities / Q000473:pathology; D051472:Elastic Cartilage / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D012399:Rotation; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D015415:Biomarkers; D002809:Chondroitin Sulfates; D003094:Collagen; D017886:Durapatite