title: Brassica oleracea L. Var. costata DC and Pieris brassicae L. aqueous extracts reduce methyl methanesulfonate-induced DNA damage in V79 hamster lung fibroblasts.
abstract: Brassica oleracea L. var. costata DC leaves and Pieris brassicae L. larvae aqueous extracts were assayed for their potential to prevent/induce DNA damage. None of them was mutagenic at the tested concentrations in the Ames test reversion assay using Salmonella His(+) TA98 strains, with and without metabolic activation. In the hypoxanthine-guanine phosphoribosyltransferase mutation assay using mammalian V79 fibroblast cell line, extracts at 500 μg/mL neither induced mutations nor protected against the mutagenicity caused by methyl methanesulfonate (MMS). In the comet assay, none of the extracts revealed to be genotoxic by itself, and both afforded protection, more pronounced for larvae extracts, against MMS-induced genotoxicity. As genotoxic/antigenotoxic effects of Brassica vegetables are commonly attributed to isothiocyanates, the extracts were screened for these compounds by headspace-solid-phase microextraction/gas chromatography-mass spectrometry. No sulfur compound was detected. These findings demonstrate that both extracts could be useful against damage caused by genotoxic compounds, the larvae extract being the most promising.
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, no. 228, 4050-313 Porto, Portugal.
authors.forename: Carla
authors.initials: C
authors.lastname: Sousa
authors.forename: Fátima
authors.initials: F
authors.lastname: Fernandes
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.forename: António Sebastião
authors.initials: AS
authors.lastname: Rodrigues
authors.forename: Marta
authors.initials: M
authors.lastname: Coelho
authors.forename: João P
authors.initials: JP
authors.lastname: Teixeira
authors.forename: Susana
authors.initials: S
authors.lastname: Silva
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: Paula
authors.initials: P
authors.lastname: Guedes de Pinho
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000494:pharmacology*; D001937:Brassica / Q000737:chemistry*; D002080:Butterflies / Q000737:chemistry*; D002460:Cell Line; D020552:Comet Assay; D006224:Cricetinae; D004249:DNA Damage / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D007814:Larva / Q000737:chemistry; D008168:Lung / Q000187:drug effects; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D001685:Biological Factors; D009153:Mutagens; D010936:Plant Extracts; D008741:Methyl Methanesulfonate