title: Chloroplast subspecies-specific SNP detection and its maternal inheritance in Brassica oleracea L. by using a dCAPS marker.
abstract: Chloroplast simple sequence repeats amplicons in 5 subspecies of Brassica oleracea were sequenced, and one chloroplast SNP was detected in amplicon ACP43. Through the introduction of an RsaI recognition site by adding one mismatch in the forward primer, combined with the increased primer length and raised annealing temperature, the dCAPS (derived cleaved amplified polymorphic sequences) marker ACP43-93 RsaI was successfully developed. By using the dCAPS marker, the subspecies-specific SNP was assayed in 206 materials representing the wide distribution of B. oleracea. This is the first report of chloroplast DNA (cpDNA) variation in cultivated subspecies of B. oleracea, which showed that chloroplast diversity existed at the intersubspecies level. Unlike other subspecies, most of the broccoli and all of the cauliflower materials sharing the same haplotype showed closer relationships in cpDNA level. Furthermore, the dCAPS haplotype of the offspring from 7 male sterile backcross populations was the same as the female parents, indicating maternal inheritance.
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Yangyong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Fang
authors.forename: Qingbiao
authors.initials: Q
authors.lastname: Wang
authors.forename: Yumei
authors.initials: Y
authors.lastname: Liu
authors.forename: Limei
authors.initials: L
authors.lastname: Yang
authors.forename: Mu
authors.initials: M
authors.lastname: Zhuang
authors.forename: Peitian
authors.initials: P
authors.lastname: Sun
mesh_terms: D019607:Brassicaceae / Q000235:genetics*; D003433:Crosses, Genetic; D018742:DNA, Chloroplast / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers; D014644:Genetic Variation; D006239:Haplotypes; D020641:Polymorphism, Single Nucleotide; D013045:Species Specificity
chemical_list: D018742:DNA, Chloroplast; D005819:Genetic Markers