title: Assessment of genetic fidelity in Rauvolfia serpentina plantlets grown from synthetic (encapsulated) seeds following in vitro storage at 4 °C.
abstract: An efficient method was developed for plant regeneration and establishment from alginate encapsulated synthetic seeds of Rauvolfia serpentina. Synthetic seeds were produced using in vitro proliferated microshoots upon complexation of 3% sodium alginate prepared in Llyod and McCown woody plant medium (WPM) and 100 mM calcium chloride. Re-growth ability of encapsulated nodal segments was evaluated after storage at 4 °C for 0, 1, 2, 4, 6 and 8 weeks and compared with non-encapsulated buds. Effects of different media viz; Murashige and Skoog medium; Lloyd and McCown woody Plant medium, Gamborg’s B5 medium and Schenk and Hildebrandt medium was also investigated for conversion into plantlets. The maximum frequency of conversion into plantlets from encapsulated nodal segments stored at 4 °C for 4 weeks was achieved on woody plant medium supplement with 5.0 μM BA and 1.0 μM NAA. Rooting in plantlets was achieved in half-strength Murashige and Skoog liquid medium containing 0.5 μM indole-3-acetic acid (IAA) on filter paper bridges. Plantlets obtained from stored synseeds were hardened, established successfully ex vitro and were morphologically similar to each other as well as their mother plant. The genetic fidelity of Rauvolfia clones raised from synthetic seeds following four weeks of storage at 4 °C were assessed by using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. All the RAPD and ISSR profiles from generated plantlets were monomorphic and comparable to the mother plant, which confirms the genetic stability among the clones. This synseed protocol could be useful for establishing a particular system for conservation, short-term storage and production of genetically identical and stable plants before it is released for commercial purposes.
authors.affiliation: Department of Botany & Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. faisalm15@yahoo.com
authors.forename: Mohammad
authors.initials: M
authors.lastname: Faisal
authors.forename: Abdulrahman A
authors.initials: AA
authors.lastname: Alatar
authors.forename: Naseem
authors.initials: N
authors.lastname: Ahmad
authors.forename: Mohammad
authors.initials: M
authors.lastname: Anis
authors.forename: Ahmad K
authors.initials: AK
authors.lastname: Hegazy
mesh_terms: D000464:Alginates; D002122:Calcium Chloride; D003470:Culture Media; D018744:DNA, Plant / Q000032:analysis*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007210:Indoleacetic Acids / Q000494:pharmacology; D018895:Microsatellite Repeats / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000502:physiology; D019105:Random Amplified Polymorphic DNA Technique; D011926:Rauwolfia / Q000235:genetics* / Q000254:growth & development; D020127:Recovery of Function; D012034:Refrigeration; D012639:Seeds / Q000235:genetics* / Q000254:growth & development
chemical_list: D000464:Alginates; D003470:Culture Media; D018744:DNA, Plant; D006603:Hexuronic Acids; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D020723:Glucuronic Acid; D002122:Calcium Chloride