title: [Application of micro-column gel cards assay for direct Coombs test in diagnosis of autoimmune hemolytic anemia].
abstract: OBJECTIVE
  To evaluate the clinic value of micro-column gel cards direct Coombs test (gel test) in diagnosis of autoimmune hemolytic anemia (AIHA).
  
  
  METHODS
  Specimens of 128 suspected AIHA patients were performed direct Coombs test by conventional tube or gel cards. The results of the two tests were compared. The hemoglobin concentrations, proportions of reticulocyte, serum levels of bilirubin and free hemoglobin were detected simultaneously and compared in subgroups.
  
  
  RESULTS
  The positive detection rate of direct Coombs test performed by gel test or tube were 88.4% and 37.7%, respectively.
  
  
  CONCLUSIONS
  Compared with the tube tests, gel test assay is more sensitive, easy to perform and standardized in diagnosis of AIHA, and the gel card can be stored for a long time. The gel test is valuable for the diagnosis of AIHA.
authors.affiliation: Department of Blood Transfusion, Chinese Academy of Medical Sciences, Tianjin, China.
authors.forename: Xue-li
authors.initials: XL
authors.lastname: Zhou
authors.forename: Shi
authors.initials: S
authors.lastname: Yan
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Zhao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D003298:Coombs Test; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D055815:Young Adult