title: Comparison of electrospray ionization, atmospheric pressure chemical ionization and atmospheric pressure photoionization for a lipidomic analysis of Leishmania donovani.
abstract: A comparison of electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI) for the analysis of a wide range of lipids has been performed on standard mixtures and extracts of Leishmania donovani promastigotes resistant to Amphotericin B (AmB). Calibration model, precision, limits of detection and quantification (LOD and LOQ) were assessed for each source. APPI provided the highest signal, signal-to-noise (S/N), and sensitivity for non-polar and low-polarity lipids, while ESI and APCI gave better results for the most polar ones. The linear model was valid for all lipids, except for one class with APPI, six classes with ESI, and eleven classes with APCI. LODs ranged from 0.2 to 20 μg mL(-1) for ESI, from 0.1 to 10 μg mL(-1) for APCI, and from 0.02 to 9.5 μg mL(-1) for APPI. LOQs ranged from 0.2 to 61 μg mL(-1) for ESI, from 0.4 to 31 μg mL(-1) for APCI, and from 0.1 to 29 μg mL(-1) for APPI. Each source provided similar lipid composition and variations in a comparison of three different L. donovani samples: miltefosine-treated, miltefosine-resistant and treated miltefosine-resistant parasites. A treated miltefosine-resistant sample was finally analyzed with each ion source in order to verify that the same lipid molecular species are detected.
authors.affiliation: Université Paris-Sud, Groupe de Chimie Analytique de Paris-Sud, EA4041, Faculté de Pharmacie, Châtenay-Malabry, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Imbert
authors.forename: Mathieu
authors.initials: M
authors.lastname: Gaudin
authors.forename: Danielle
authors.initials: D
authors.lastname: Libong
authors.forename: David
authors.initials: D
authors.lastname: Touboul
authors.forename: Sonia
authors.initials: S
authors.lastname: Abreu
authors.forename: Philippe M
authors.initials: PM
authors.lastname: Loiseau
authors.forename: Olivier
authors.initials: O
authors.lastname: Laprévote
authors.forename: Pierre
authors.initials: P
authors.lastname: Chaminade
mesh_terms: D001274:Atmospheric Pressure; D004351:Drug Resistance; D007893:Leishmania donovani / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D057230:Limit of Detection; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D055442:Metabolome / Q000187:drug effects; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000494:pharmacology; D010777:Photochemistry / Q000379:methods*; D015203:Reproducibility of Results; D059629:Signal-To-Noise Ratio; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D008055:Lipids; D010767:Phosphorylcholine; C039128:miltefosine