title: Identification of the up-regulation of TP-alpha, collagen alpha-1(VI) chain, and S100A9 in esophageal squamous cell carcinoma by a proteomic method.
abstract: Esophageal squamous cell carcinoma (ESCC) is one of the most common primary malignant tumor of digestive tract. However, the early diagnosis and molecular mechanisms that underlie tumor formation and progression have been progressed less. To identify new biomarkers for ESCC, we performed a comparative proteomic research. Isobaric tags for relative and absolute quantitation-based proteomic method was used to screen biomarkers between ESCC and normal. 802 non-redundant proteins were identified, 39 of which were differentially expressed with 1.5-fold difference (29 up-regulated and 10 down-regulated). Through Swiss-Prot and GO database, the location and function of differential proteins were analyzed, which are related to the biological processes of binding, cell structure, signal transduction, cell adhesion, etc. Among the differentially expressed proteins, TP-alpha, collagen alpha-1(VI) chain and S100A9 were verified to be upregulated in 77.19%, 75.44% and 59.65% of ESCC by immunohistochemistry and western-blot. Diagnostic value of these three proteins was validated. These results provide new insights into ESCC biology and potential diagnostic and therapeutic biomarkers, which suggest that TP-alpha, collagen alpha-1(VI) chain and S100A9 are potential biomarkers of ESCC, and may play an important role in tumorigenesis and development of ESCC.
authors.affiliation: Institute of Anal-Colorectal Surgery, No. 150 Central Hospital of PLA, Luoyang, China.
authors.forename: Nai-Jun
authors.initials: NJ
authors.lastname: Fan
authors.forename: Chun-Fang
authors.initials: CF
authors.lastname: Gao
authors.forename: Chang-Song
authors.initials: CS
authors.lastname: Wang
authors.forename: Guang
authors.initials: G
authors.lastname: Zhao
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Lv
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Wang
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Chu
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.forename: Xu-Tao
authors.initials: XT
authors.lastname: Yuan
authors.forename: Nian-Long
authors.initials: NL
authors.lastname: Meng
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism; D015153:Blotting, Western; D040502:Calgranulin B / Q000096:biosynthesis / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D024142:Collagen Type VI / Q000096:biosynthesis / Q000097:blood*; D004938:Esophageal Neoplasms / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D063991:Mitochondrial Trifunctional Protein; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000097:blood*; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D040502:Calgranulin B; D024142:Collagen Type VI; D009097:Multienzyme Complexes; D063991:Mitochondrial Trifunctional Protein