title: Influence of pesticides on the pH regulatory enzyme, carbonic anhydrase, from European Seabass liver and bovine erythrocytes.
abstract: The objective of this study was to assess the inhibitory effects of six commonly used pesticides, cyhalothrin, cypermethrin, dichlorvos, methamidophos, chlorpyrifos and methylparathion, on the pH regulatory enzyme carbonic anhydrase (CA) of Dicentrarchus labrax (European Seabass) liver (dCA) and bovine erythrocytes (bCA). Results of the study showed that the pesticides displayed quite variable inhibition profiles with K(I) values ranging from 0.376 to 26.164μM against dCA, and from 1.174 to 53.281μM against bCA. Methylparathion was the most effective inhibitor for both enzymes. Overall data show that all of the tested pesticides inhibit both dCA and bCA at low concentrations indicating that indiscriminate use of these pesticides might cause disruption of acid base regulation resulting in animal deaths. Our results also point out that susceptibility to these pesticides varies among CAs from different organisms.
authors.affiliation: Ataturk University, Science Faculty, Department of Chemistry, 25240, Erzurum, Turkey.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Demirdağ
authors.affiliation: Ataturk University, Science Faculty, Department of Chemistry, 25240, Erzurum, Turkey.
authors.forename: Emrah
authors.initials: E
authors.lastname: Yerlikaya
authors.affiliation: Atatürk University, Agriculture Faculty, Department of Agricultural Biotechnology, 25240 Erzurum, Turkey.
authors.forename: Ercüment
authors.initials: E
authors.lastname: Aksakal
authors.affiliation: Ataturk University, Science Faculty, Department of Chemistry, 25240, Erzurum, Turkey. Electronic address: okufrevi@atauni.edu.tr.
authors.forename: Ömer Irfan
authors.initials: ÖI
authors.lastname: Küfrevioğlu
authors.affiliation: Ondokuz Mayıs University, Agricultural Faculty, Department of Agricultural Biotechnology, Enzyme and Microbial Biotechnology Division, 55139 Samsun, Turkey. Electronic address: deniz.ekinci@omu.edu.tr.
authors.forename: Deniz
authors.initials: D
authors.lastname: Ekinci
mesh_terms: D000818:Animals; D001492:Bass; D002257:Carbonic Anhydrase Inhibitors / Q000633:toxicity*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002417:Cattle; D004390:Chlorpyrifos / Q000633:toxicity; D004006:Dichlorvos / Q000633:toxicity; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D004950:Esterases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008743:Methyl Parathion / Q000633:toxicity; D009570:Nitriles / Q000633:toxicity; D009946:Organothiophosphorus Compounds / Q000633:toxicity; D010575:Pesticides / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D009570:Nitriles; D009946:Organothiophosphorus Compounds; D010575:Pesticides; D011722:Pyrethrins; C017160:cypermethrin; D008743:Methyl Parathion; D004006:Dichlorvos; C014655:methamidophos; D004950:Esterases; D002256:Carbonic Anhydrases; D004390:Chlorpyrifos; C037304:cyhalothrin