title: Network-based identification of novel connections among apoptotic signaling pathways in cancer.
abstract: MicroRNAs (miRNAs), highly conserved, non-coding endogenous RNA and nearly ~22 nucleotides (nt) in length, are well-known to regulate several apoptotic pathways in cancer. In this study, we computationally constructed the initial human apoptotic PPI network by several online databases, and further integrated these high-throughput datasets into a Naïve Bayesian model to predict protein functional connections. Based on the modified apoptotic network, we identified several apoptotic hub proteins such as TP53, SRC, M3K3/5/8, cyclin-dependent kinase2/6, TNFR16/19, and TGF-β receptor 1/2. Subsequently, we identified some microRNAs that could target the aforementioned apoptotic hub proteins by using TargetScan, PicTar, and Diana-MicroH. In conclusion, these results demonstrate the PPI network-based identification of new connections amongst apoptotic pathways in cancer, which may shed new light on the intricate relationships between core apoptotic pathways and some targeted miRNAs in human cancers.
authors.affiliation: School of Life Sciences and The State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610064, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Huai-long
authors.initials: HL
authors.lastname: Xu
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Wen
authors.forename: Feng-tian
authors.initials: FT
authors.lastname: Wang
authors.forename: Shu-ya
authors.initials: SY
authors.lastname: Wang
authors.forename: Lei-lei
authors.initials: LL
authors.lastname: Fu
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Jin-ku
authors.initials: JK
authors.lastname: Bao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D060066:Protein Interaction Maps; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D035683:MicroRNAs; D016159:Tumor Suppressor Protein p53; D016392:Proto-Oncogene Proteins pp60(c-src)