title: Automated whole-cell patch-clamp electrophysiology of neurons in vivo.
abstract: Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput and quality of automated intracellular recording in mouse cortex and hippocampus.
authors.affiliation: Media Lab, Massachusetts Institute of Technology, Cambridge, USA.
authors.forename: Suhasa B
authors.initials: SB
authors.lastname: Kodandaramaiah
authors.forename: Giovanni Talei
authors.initials: GT
authors.lastname: Franzesi
authors.forename: Brian Y
authors.initials: BY
authors.lastname: Chow
authors.forename: Edward S
authors.initials: ES
authors.lastname: Boyden
authors.forename: Craig R
authors.initials: CR
authors.lastname: Forest
mesh_terms: D000465:Algorithms; D000818:Animals; D057205:Automation, Laboratory / Q000379:methods*; D002540:Cerebral Cortex / Q000166:cytology; D055724:Electrophysiological Phenomena; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods*; D012371:Robotics