title: [Finding targets of transcriptional regulators--chromatin immunoprecipitation assay (ChIP)].
abstract: The protein-DNA interactions play a crucial role in many cellular processes such as DNA replication, recombination, repair, or gene transcription. Here we describe a chromatin immunoprecipitation (ChIP) technique, a tool for identifying DNA regions, which are in vivo associated with protein of interest. The main steps of ChIP are: fixation, chromatin fragmentation, immunoprecipitation with a specific antibody, and analysis of the immunoprecipitated DNA. Different analytical tools combined with ChIP (ChIP-chip, ChIP-seq) enable researchers to map protein binding in a truly genome-wide manner with high resolution.
authors.affiliation: Biotechnology Department, Branch Campus of the Faculty of Biotechnology, Rzeszów University, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kus-Liśkiewicz
authors.forename: Wiesława
authors.initials: W
authors.lastname: Widłak
mesh_terms: D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004247:DNA / Q000378:metabolism; D010449:Peptide Mapping; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D002843:Chromatin; D014157:Transcription Factors; D004247:DNA