title: Stimulation of liver regeneration after hepatectomy in mice by injection of bone marrow mesenchymal stem cells via the portal vein.
abstract: OBJECTIVE
  To investigate whether mouse bone marrow mesenchymal stem cells (BMC) stimulate liver regeneration after partial hepatectomy.
  
  
  METHODS
  Isolated BMCs were purified by density gradient centrifugation. We performed a 70% hepatectomy in male BALB/c mice followed by injection of BMCs into the portal vein (PV-BMC group), or the tail vein (IV-BMC group), or of saline into the portal vein (control group).
  
  
  RESULTS
  The wet weight of the liver remnant increased significantly in the PV-BMC group at 3 and 5 days after hepatectomy compared with the IV-BMC and control groups. The Ki-67 labeling index revealed that the increase to result from stimulation of DNA synthesis. The constitutive interleukin-6 and hepatocyte growth factor mRNAs in the remnant liver tended to increase in the PV-BMC group at 3 days after hepatectomy.
  
  
  CONCLUSIONS
  These results demonstrated that BMC injection into the portal vein enhanced liver growth after partial hepatectomy in mice.
authors.affiliation: Department of Surgery, Kansai Medical University, Osaka, Japan. kaibori@hirakata.kmu.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Kaibori
authors.forename: Y
authors.initials: Y
authors.lastname: Adachi
authors.forename: T
authors.initials: T
authors.lastname: Shimo
authors.forename: M
authors.initials: M
authors.lastname: Ishizaki
authors.forename: K
authors.initials: K
authors.lastname: Matsui
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Ohishi
authors.forename: Y
authors.initials: Y
authors.lastname: Araki
authors.forename: T
authors.initials: T
authors.lastname: Okumura
authors.forename: M
authors.initials: M
authors.lastname: Nishizawa
authors.forename: A H
authors.initials: AH
authors.lastname: Kwon
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D049109:Cell Proliferation; D004261:DNA Replication; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000235:genetics; D007275:Injections, Intravenous; D015850:Interleukin-6 / Q000235:genetics; D019394:Ki-67 Antigen / Q000378:metabolism; D008099:Liver / Q000098:blood supply / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D008115:Liver Regeneration; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011169:Portal Vein; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D015850:Interleukin-6; D019394:Ki-67 Antigen; D012333:RNA, Messenger; D017228:Hepatocyte Growth Factor