title: miR-15a and miR-16 regulate serotonin transporter expression in human placental and rat brain raphe cells.
abstract: The serotonin transporter (SERT) is a key regulatory molecule in serotonergic transmission implicated in numerous biological processes relevant to human disorders. Recently, it was shown that SERT expression is controlled by miR-16 in mouse brain. Here, we show that SERT expression is regulated additionally by miR-15a as well as miR-16 in human and rat tissues. This post-transcriptional regulation was observed and characterized in reporter assays and likewise when endogenous SERT expression was evaluated in human placental choriocarcinoma JAR cells and rat brain raphe RN46A cells - two cell lines that endogenously express SERT. Similar effects for miR-16 to those of miR-15a were found in both human and rat cell lines. The effects of miR-15a and miR-16 were comparable in extent to those originally reported for miR-16 in mice. These findings represent a novel layer of complexity for SERT expression regulation exerted by the mir-15a/16 cluster, whose genes are adjacently located at human chromosome 13q14.3.
authors.affiliation: Laboratory of Clinical Science, NIMH Intramural Research Program, NIH, Bethesda, MD 20892, USA. moyap@mail.nih.gov
authors.forename: Pablo R
authors.initials: PR
authors.lastname: Moya
authors.forename: Jens R
authors.initials: JR
authors.lastname: Wendland
authors.forename: Jennifer
authors.initials: J
authors.lastname: Salemme
authors.forename: Ruby L
authors.initials: RL
authors.lastname: Fried
authors.forename: Dennis L
authors.initials: DL
authors.lastname: Murphy
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D008969:Molecular Sequence Data; D010920:Placenta / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D011903:Raphe Nuclei / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D050486:Serotonin Plasma Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C505361:MIRN15 microRNA, human; C505362:MIRN16 microRNA, human; D035683:MicroRNAs; C493230:SLC6A4 protein, human; D050486:Serotonin Plasma Membrane Transport Proteins