title: The inhibitory effect of miRNA-1 on ET-1 gene expression.
abstract: There is a close correlation between endothelin-1 (ET-1) and microRNA-1 (miRNA-1) expression in the heart, but whether ET-1 expression is regulated by miRNA-1 warrants further research. Our results revealed multiple clues suggesting that miRNA-1 may participate in inhibiting ET-1 gene expression. The inhibitory effect of miRNA-1 on recombinant luciferase reporter gene was mediated by the target sequence at the 127th nucleotide on ET-1 3'UTR. We further confirmed that miRNA-1 could inhibit endogenous ET-1 gene expression at the post-transcriptional level. Our study provides a new perspective on the regulatory mechanism of ET-1 gene.
authors.affiliation: Department of Physiology, Second Military Medical University, Shanghai 200433, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.forename: Shi-Fu
authors.initials: SF
authors.lastname: Shan
authors.forename: Qing
authors.initials: Q
authors.lastname: Liang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Yuan
authors.forename: An-Jing
authors.initials: AJ
authors.lastname: Ren
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002478:Cells, Cultured; D015536:Down-Regulation; D019332:Endothelin-1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D008958:Models, Molecular; D050505:Mutant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D009154:Mutation; D032446:Myoblasts / Q000378:metabolism*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D019332:Endothelin-1; C544547:MIRN1 microRNA, human; D035683:MicroRNAs; C517731:Mirn1 microRNA, mouse; D050505:Mutant Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins