title: Methanothermobacter tenebrarum sp. nov., a hydrogenotrophic, thermophilic methanogen isolated from gas-associated formation water of a natural gas field.
abstract: A thermophilic and hydrogenotrophic methanogen, strain RMAS(T), was isolated from gas-associated formation water of a gas-producing well in a natural gas field in Japan. Strain RMAS(T) grew solely on H(2)/CO(2) but required Casamino acids, tryptone, yeast extract or vitamins for growth. Growth of strain RMAS(T) was stimulated by acetate. Cells were non-motile, straight rods (0.5×3.5-10.5 µm) and occurred singly or in pairs. Bundles of fimbriae occurred at both poles of cells and the cell wall was thick (approximately 21 nm, as revealed by ultrathin section electron microscopy). Strain RMAS(T) grew at 45-80 °C (optimum, 70 °C), at pH 5.8-8.7 (optimum, pH 6.9-7.7) and with 0.001-20 g NaCl l(-1) (optimum, 2.5 g NaCl l(-1)). Phylogenetic analysis revealed that Methanothermobacter thermautotrophicus ΔH(T) was most closely related to the isolate (95.7 % 16S rRNA gene sequence similarity). On the basis of morphological, phenotypic and phylogenetic characteristics, it is clear that strain RMAS(T) represents a novel species of the genus Methanothermobacter, for which we propose the name Methanothermobacter tenebrarum sp. nov. The type strain is RMAS(T) ( = DSM 23052(T) = JCM 16532(T) = NBRC 106236(T)).
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.|Faculty of Applied Biological Sciences, Gifu University, Yanagido, Gifu 501-1193, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Graduate School of Applied Biological Sciences, Gifu University, Yanagido, Gifu 501-1193, Japan.
authors.forename: Azumi
authors.initials: A
authors.lastname: Takahashi
authors.affiliation: Graduate School of Applied Biological Sciences, Gifu University, Yanagido, Gifu 501-1193, Japan.
authors.forename: Chikahiro
authors.initials: C
authors.lastname: Mori
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tamaki
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Hanako
authors.initials: H
authors.lastname: Mochimaru
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nakamura
authors.affiliation: Faculty of Applied Biological Sciences, Gifu University, Yanagido, Gifu 501-1193, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takamizawa
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido 062-8517, Japan.|Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamagata
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D007564:Japan; D008055:Lipids / Q000032:analysis; D008697:Methane / Q000378:metabolism; D017013:Methanobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D059206:Natural Gas / Q000382:microbiology; D061329:Oil and Gas Fields / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D008055:Lipids; D059206:Natural Gas; D012336:RNA, Ribosomal, 16S; D008697:Methane