title: The dynamic expression of Mash1 in the hippocampal subgranular zone after fimbria-fornix transection.
abstract: Mash1, a member of the basic helix-loop-helix (bHLH) transcription factor family, has previously been considered essential for neuronal differentiation and specification in the nervous system. In this study, we investigated the expression of Mash1 in the hippocampus after fimbria-fornix (FF) transection. Western blot showed that protein of Mash1 increased significantly and peaked at day 7 after FF transection. Immunofluorescence indicated that after FF transection, more newborn cells differentiated into Mash1 positive cells in the deafferented side than that in the normal side, and we investigated that in the neurogenic area, subgranular zone (SGZ), a part of Mash1 positive cells were NeuN positive, and more Mash1/NeuN double positive neurons were identified in the deafferented side than that in the normal side. Additionally, the number of Mash1/NeuN double positive neurons in SGZ increased significantly and peaked at day 7 after FF transection. In vitro, immunofluorescence revealed that extracts of the deafferented hippocampus promoted neuronal differentiation to a greater extent than extracts from normal hippocampus. Deafferented extracts also enhanced Mash1 expression in MAP-2 positive neurons. This study concludes that after FF transection, Mash1 expression in the deafferented hippocampus increased and might play an important role in inducing local progenitors to differentiate into neurons.
authors.affiliation: Department of Anatomy and Neurobiology, the Jiangsu Key Laboratory of Neuroregeneration, Nantong University, People's Republic of China.
authors.forename: Chuanming
authors.initials: C
authors.lastname: Dong
authors.forename: Heyan
authors.initials: H
authors.lastname: Zhao
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhang
authors.forename: Jinhong
authors.initials: J
authors.lastname: Shi
authors.forename: Haoming
authors.initials: H
authors.lastname: Li
authors.forename: Guohua
authors.initials: G
authors.lastname: Jin
mesh_terms: D000344:Afferent Pathways / Q000166:cytology; D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D005260:Female; D020712:Fornix, Brain / Q000293:injuries; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D034961:Antigens, Nuclear; D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; C499318:Ascl1 protein, rat; C546339:Rbfox3 protein, rat