title: Associations of yeasts with spotted-wing Drosophila (Drosophila suzukii; Diptera: Drosophilidae) in cherries and raspberries.
abstract: A rich history of investigation documents various Drosophila-yeast mutualisms, suggesting that Drosophila suzukii similarly has an association with a specific yeast species or community. To discover candidate yeast species, yeasts were isolated from larval frass, adult midguts, and fruit hosts of D. suzukii. Terminal restriction fragment length polymorphism (TRFLP) technology and decimal dilution plating were used to identify and determine the relative abundance of yeast species present in fruit juice samples that were either infested with D. suzukii or not infested. Yeasts were less abundant in uninfested than infested samples. A total of 126 independent yeast isolates were cultivated from frass, midguts, and fruit hosts of D. suzukii, representing 28 species of yeasts, with Hanseniaspora uvarum predominating. This suggests an association between D. suzukii and H. uvarum that could be utilized for pest management of the highly pestiferous D. suzukii.
authors.affiliation: Department of Entomology and Nematology, University of California, Davis, Davis, CA, USA. kahamby@ucdavis.edu
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Hamby
authors.forename: Alejandro
authors.initials: A
authors.lastname: Hernández
authors.forename: Kyria
authors.initials: K
authors.lastname: Boundy-Mills
authors.forename: Frank G
authors.initials: FG
authors.lastname: Zalom
mesh_terms: D000818:Animals; D018556:Crops, Agricultural / Q000254:growth & development / Q000469:parasitology*; D004330:Drosophila / Q000145:classification / Q000254:growth & development / Q000382:microbiology*; D005638:Fruit / Q000254:growth & development / Q000469:parasitology*; D010935:Plant Diseases / Q000469:parasitology; D012150:Polymorphism, Restriction Fragment Length; D004718:Saccharomycetales / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D013559:Symbiosis; D015003:Yeasts / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification