title: Activation of rabbit TLR9 by different CpG-ODN optimized for mouse and human TLR9.
abstract: Synthetic CpG-oligodeoxynucleotides (CpG-ODN) are potent adjuvants that accelerate and boost antigen-specific immune responses. Toll-like receptor 9 (TLR9) is the cellular receptor for these CpG-ODN. Previous studies have shown species-specific activation of mouse TLR9 (mTLR9) and human TLR9 (hTLR9) by their optimized CpG-ODN. The interaction between rabbit TLR9 (rabTLR9) and CpG-ODN, however, has not been previously investigated. Here, we cloned and characterized rabTLR9 and comparatively investigated the activation of the rabbit, mouse, and human TLR9 by CpG-ODN. The complete open reading frame of rabTLR9 encodes 1028 amino acid residues, which share 70.6% and 75.5% of the identities of mTLR9 and hTLR9, respectively. Rabbit TLR9 is preferentially expressed in immune cells rich tissues, and is localized in intracellular vesicles. While mTLR9 and hTLR9 displayed species-specific recognition of their optimized CpG-ODN, rabbit TLR9 was activated by these CpG-ODN without any preference. This result suggests that rabTLR9 has a broader ligand-recognition profile than mouse and human TLR9.
authors.affiliation: Infectious and Inflammatory Disease Center, Sanford-Burnham Medical Research Institute, La Jolla, CA 92037, United States.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Congfeng
authors.initials: C
authors.lastname: Xu
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Liu
authors.forename: Hanako
authors.initials: H
authors.lastname: Matsuo
authors.forename: Rebecca Pe-feng
authors.initials: RP
authors.lastname: Hsieh
authors.forename: Jeng-Fan
authors.initials: JF
authors.lastname: Lo
authors.forename: Ping-Hui
authors.initials: PH
authors.lastname: Tseng
authors.forename: Chiun-Jye
authors.initials: CJ
authors.lastname: Yuan
authors.forename: Yunping
authors.initials: Y
authors.lastname: Luo
authors.forename: Rong
authors.initials: R
authors.lastname: Xiang
authors.forename: Tsung-Hsien
authors.initials: TH
authors.lastname: Chuang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000145:classification / Q000494:pharmacology*; D016366:Open Reading Frames; D010802:Phylogeny; D011817:Rabbits; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014018:Tissue Distribution; D051217:Toll-Like Receptor 9 / Q000378:metabolism*
chemical_list: C408982:CPG-oligonucleotide; D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; C495371:TLR9 protein, human; C495370:Tlr9 protein, mouse; D051217:Toll-Like Receptor 9