title: Enhancing production of bio-isoprene using hybrid MVA pathway and isoprene synthase in E. coli.
abstract: The depleting petroleum reserve, increasingly severe energy crisis, and global climate change are reigniting enthusiasm for seeking sustainable technologies to replace petroleum as a source of fuel and chemicals. In this paper, the efficiency of the MVA pathway on isoprene production has been improved as follows: firstly, in order to increase MVA production, the source of the "upper pathway" which contains HMG-CoA synthase, acetyl-CoA acetyltransferase and HMG-CoA reductase to covert acetyl-CoA into MVA has been changed from Saccharomyces cerevisiae to Enterococcus faecalis; secondly, to further enhance the production of MVA and isoprene, a alanine 110 of the mvaS gene has been mutated to a glycine. The final genetic strain YJM25 containing the optimized MVA pathway and isoprene synthase from Populus alba can accumulate isoprene up to 6.3 g/L after 40 h of fed-batch cultivation.
authors.affiliation: Biomaterials Center, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Yang
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
authors.forename: Sizheng
authors.initials: S
authors.lastname: Su
authors.forename: Guang
authors.initials: G
authors.lastname: Zhao
authors.forename: Qingjuan
authors.initials: Q
authors.lastname: Nie
authors.forename: Xinglin
authors.initials: X
authors.lastname: Jiang
authors.forename: Yanning
authors.initials: Y
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D000101:Acetyl-CoA C-Acetyltransferase / Q000378:metabolism; D019883:Alkyl and Aryl Transferases / Q000378:metabolism*; D056804:Biofuels; D002070:Butadienes; D002849:Chromatography, Gas; D017931:DNA Primers / Q000235:genetics; D013293:Enterococcus faecalis / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005285:Fermentation; D045782:Hemiterpenes / Q000096:biosynthesis*; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000378:metabolism; D008798:Mevalonic Acid / Q000378:metabolism*; D010420:Pentanes; D010957:Plasmids / Q000235:genetics; D032107:Populus / Q000201:enzymology
chemical_list: D056804:Biofuels; D002070:Butadienes; D017931:DNA Primers; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene; D000101:Acetyl-CoA C-Acetyltransferase; D006904:Hydroxymethylglutaryl-CoA Synthase; D019883:Alkyl and Aryl Transferases; C093854:isoprene synthase; D008798:Mevalonic Acid