title: In vitro activity of mastoparan-AF alone and in combination with clinically used antibiotics against multiple-antibiotic-resistant Escherichia coli isolates from animals.
abstract: The in vitro activity of mastoparan-AF, an amphipathic antimicrobial peptide isolated from the hornet venom of Vespa affinis, alone and in combination with various clinically used antibiotics, was investigated against 21 Escherichia coli isolates/strains. Most E. coli isolates tested were detected containing multiple-antimicrobial resistance genes. Antimicrobial activity of mastoparan-AF was measured by MIC, MBC, time-kill kinetic assay and chequerboard titration method. Mastoparan-AF exhibited potent antimicrobial activity against most multiple-antibiotic-resistant E. coli isolates at the concentrations ranging from 4 to 16 μg/ml. Combination studies showed that mastoparan-AF acts synergistically with certain antibiotics, i.e., cephalothin or gentamicin, against some multiple-antibiotic-resistant E. coli isolates. In conclusion, mastoparan-AF alone or in combination with other antibiotics could be promising as alternatives for combating multiple-antibiotic-resistant E. coli in future clinical applications.
authors.affiliation: Department of Entomology, National Chung Hsing University, Taichung 40227, Taiwan, ROC.
authors.forename: Chun-Hsien
authors.initials: CH
authors.lastname: Lin
authors.forename: Roger F
authors.initials: RF
authors.lastname: Hou
authors.forename: Ching-Lin
authors.initials: CL
authors.lastname: Shyu
authors.forename: Wei-Yau
authors.initials: WY
authors.lastname: Shia
authors.forename: Chuen-Fu
authors.initials: CF
authors.lastname: Lin
authors.forename: Wu-Chun
authors.initials: WC
authors.lastname: Tu
mesh_terms: D000595:Amino Acid Sequence; D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002512:Cephalothin / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005798:Genes, Bacterial; D005839:Gentamicins / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009355:Neomycin / Q000494:pharmacology; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D013752:Tetracycline / Q000494:pharmacology; D014862:Wasp Venoms / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D005839:Gentamicins; D014862:Wasp Venoms; C575417:mastoparan-AF; D002701:Chloramphenicol; D000667:Ampicillin; D013752:Tetracycline; D009355:Neomycin; D002512:Cephalothin