title: Rendering protein-based particles transiently insoluble for therapeutic applications.
abstract: Herein, we report the fabrication of protein (bovine serum albumin, BSA) particles which were rendered transiently insoluble using a novel, reductively labile disulfide-based cross-linker. After being cross-linked, the protein particles retain their integrity in aqueous solution and dissolve preferentially under a reducing environment. Our data demonstrates that cleavage of the cross-linker leaves no chemical residue on the reactive amino group. Delivery of a self-replicating RNA was achieved via the transiently insoluble PRINT protein particles. These protein particles can provide new opportunities for drug and gene delivery.
authors.affiliation: Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Luft
authors.forename: Shaomin
authors.initials: S
authors.lastname: Tian
authors.forename: Gary
authors.initials: G
authors.lastname: Owens
authors.forename: Ashish A
authors.initials: AA
authors.lastname: Pandya
authors.forename: Peter
authors.initials: P
authors.lastname: Berglund
authors.forename: Patrick
authors.initials: P
authors.lastname: Pohlhaus
authors.forename: Benjamin W
authors.initials: BW
authors.lastname: Maynor
authors.forename: Jonathan
authors.initials: J
authors.lastname: Smith
authors.forename: Bolyn
authors.initials: B
authors.lastname: Hubby
authors.forename: Mary E
authors.initials: ME
authors.lastname: Napier
authors.forename: Joseph M
authors.initials: JM
authors.lastname: DeSimone
mesh_terms: D000818:Animals; D002417:Cattle; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D055616:Microtechnology / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D012313:RNA / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D012995:Solubility; D013997:Time Factors; D014709:Vero Cells
chemical_list: D004220:Disulfides; D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D012313:RNA