title: Identification of Chinese cabbage sentrin as a suppressor of Bax-induced cell death in yeast.
abstract: Studies into the cell death program termed apoptosis have resulted in new information regarding how cells control and execute their own demise, including insights into the mechanism by which death-preventing factors can inhibit Bax-induced caspase activation. We investigated high temperature stress-induced cell death in Brassica rapa. Using a yeast functional screening from a Brassica rapa cDNA library, the BH5-127 EST clone encoding an apoptotic suppressor peptide was identified. However, a phylogenic tree showed that BH5-127 clusters within a clade containing SUMO-1 (Small Ubiquitin-like Modifier- 1). BH5-127 was confirmed similar to have function to SUMO-1 as Fas suppression. Expression of BH5-127 showed that substantial suppression of cell death survived on SD-galactose-Leu--Ura- medium. The results suggest that BrSE (Brassica rapa Sentrin EST, BH5-127) is one of the important regulatory proteins in programming cell death, especially in the seedling stage of Chinese cabbage.
authors.affiliation: School of Applied Ecological Resources, College of Ecology and Environmental Science, Kyungpook National University, Sangju, 742-711, Korea.
authors.forename: Widhi Dyah
authors.initials: WD
authors.lastname: Sawitri
authors.forename: Slameto
authors.initials: S
authors.lastname: Slameto
authors.forename: Bambang
authors.initials: B
authors.lastname: Sugiharto
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D029741:Brassica rapa / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism* / Q000494:pharmacology; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D025842:SUMO-1 Protein; D051028:bcl-2-Associated X Protein