title: Improved purity and immunostimulatory activity of β-(1→3)(1→6)-glucan from Pleurotus sajor-caju using cell wall-degrading enzymes.
abstract: The objective of this work was to improve the purity of β-(1→3)(1→6)-glucan in the native triple helical structure from the fruiting bodies of Pleurotus sajor-caju for effective biological function using cell wall-degrading enzymes. A crude carbohydrate was extracted with hot water, then treated with crude xylanase and cellulase from Paenibacillus curdlanolyticus B-6. β-Glucan in the extract was purified to homogeneity with a single and symmetrical peak using 650M DEAE Toyopearl and Sepharose CL-6B column chromatography. The purity of β-glucan was confirmed by high-performance size-exclusion chromatography. Purified β-glucan was obtained at a purity of up to 90.2%. The Congo red reaction and atomic force microscopy indicated that the purified β-glucan exhibited a triple helix conformation. Purified β-glucan was able to effectively up-regulate the functions of macrophages such as nitric oxide (NO) and tumor necrosis factor (TNF-α) production.
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
authors.forename: Saranya
authors.initials: S
authors.lastname: Satitmanwiwat
authors.forename: Khanok
authors.initials: K
authors.lastname: Ratanakhanokchai
authors.forename: Natta
authors.initials: N
authors.lastname: Laohakunjit
authors.forename: Louis Kuoping
authors.initials: LK
authors.lastname: Chao
authors.forename: Shui-Tein
authors.initials: ST
authors.lastname: Chen
authors.forename: Patthra
authors.initials: P
authors.lastname: Pason
authors.forename: Chakrit
authors.initials: C
authors.lastname: Tachaapaikoon
authors.forename: Khin Lay
authors.initials: KL
authors.lastname: Kyu
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry*; D002460:Cell Line; D002473:Cell Wall / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005936:Glucans / Q000737:chemistry / Q000494:pharmacology*; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D056507:Paenibacillus / Q000201:enzymology*; D020076:Pleurotus / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D005936:Glucans; D007155:Immunologic Factors; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases