title: Effect of phosphate deficiency on the content and biosynthesis of anthocyanins and the expression of related genes in suspension-cultured grape (Vitis sp.) cells.
abstract: The effect of phosphate (Pi) starvation on anthocyanin biosynthesis was examined in suspension-cultured grape cells derived from Bailey Alicante A grapes. Cyanindin-3-O-glucoside, peonidin-3-O-glucoside, cyanidin-3-O-(6-O״-p-coumaroyl)-glucoside and peonidin-3-O-(6-O״-p-coumaroyl)-glucoside were detected in cells in both the complete (+Pi)- and Pi-deficient Murashige-Skoog (-Pi) medium but accumulation was much more substantial cells in -Pi cultures. Highest incorporation of [ring-(14)C]phenylalanine into anthocyanins was found at days 3-14 in the cells in -Pi medium. Time-course expression profiles of anthocyanin biosynthetic enzyme genes and a regulatory gene were examined after cells were transferred to the +Pi and -Pi medium. The transcript levels of UFGT and VvmybA1 in -Pi cells were higher than in +Pi cells 24 h after transfer coinciding with the start of anthocyanin accumulation in -Pi cells.
authors.affiliation: Department of Biological Sciences, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Yin
authors.forename: Gina
authors.initials: G
authors.lastname: Borges
authors.forename: Masaaki
authors.initials: M
authors.lastname: Sakuta
authors.forename: Alan
authors.initials: A
authors.lastname: Crozier
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ashihara
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000378:metabolism / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D010649:Phenylalanine / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D027843:Vitis / Q000166:cytology / Q000235:genetics / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D003470:Culture Media; D010710:Phosphates; D010940:Plant Proteins; D010649:Phenylalanine; D005964:Glucosyltransferases; C023249:flavonol 3-O-glucosyltransferase