title: Allelic diversity at the DLA-88 locus in Golden Retriever and Boxer breeds is limited.
abstract: In the dog, previous analyses of major histocompatibility complex class I genes suggest a single polymorphic locus, dog leukocyte antigen (DLA)-88. While 51 alleles have been reported, estimates of prevalence have not been made. We hypothesized that, within a breed, DLA-88 diversity would be restricted, and one or more dominant alleles could be identified. Accordingly, we determined allele usage in 47 Golden Retrievers and 39 Boxers. In each population, 10 alleles were found; 4 were shared. Seven novel alleles were identified. DLA-88*05101 and *50801 predominated in Golden Retrievers, while most Boxers carried *03401. In these breeds, DLA-88 polymorphisms are limited and largely non-overlapping. The finding of highly prevalent alleles fulfills an important prerequisite for studying canine CD8+ T-cell responses.
authors.affiliation: Department of Clinical Sciences, and Immunology Program, North Carolina State University College of Veterinary Medicine, Raleigh, NC 27607, USA.
authors.forename: P
authors.initials: P
authors.lastname: Ross
authors.forename: A S
authors.initials: AS
authors.lastname: Buntzman
authors.forename: B G
authors.initials: BG
authors.lastname: Vincent
authors.forename: E N
authors.initials: EN
authors.lastname: Grover
authors.forename: G S
authors.initials: GS
authors.lastname: Gojanovich
authors.forename: E J
authors.initials: EJ
authors.lastname: Collins
authors.forename: J A
authors.initials: JA
authors.lastname: Frelinger
authors.forename: P R
authors.initials: PR
authors.lastname: Hess
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001947:Breeding; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D004285:Dogs / Q000235:genetics / Q000276:immunology*; D005787:Gene Frequency; D056426:Genetic Loci; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D006720:Homozygote; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016415:Sequence Alignment
chemical_list: D015395:Histocompatibility Antigens Class I