title: Is manganese (II) pentaazamacrocyclic superoxide dismutase mimic beneficial for human sperm mitochondria function and motility?
abstract: Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes in sperm redox milieu affect the functional status of sperm mitochondria, that is, sperm functionality. Compared with the control, incubation in Tyrode's medium for 3 h, under noncapacitating conditions, decreased sperm motility, the amount of nitric oxide ((•)NO), the number of MitoTracker(®) Green FM (MT-G) positive mitochondria, and the expression of complexes I and IV of the mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic (M40403) treatment restored/increased these parameters, as well as the expression of endothelial nitric oxide synthase, manganese SOD, and catalase. These data lead to the hypothesis that M40403 improves mitochondrial functional state and motility of spermatozoa, as well as (•)NO might be involved in the observed effects of the mimic.
authors.affiliation: Department of Physiology, Institute for Biological Research Sinisa Stankovic, University of Belgrade, Belgrade, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Otasevic
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Korac
authors.forename: Milica
authors.initials: M
authors.lastname: Vucetic
authors.forename: Biljana
authors.initials: B
authors.lastname: Macanovic
authors.forename: Eliana
authors.initials: E
authors.lastname: Garalejic
authors.forename: Ivana
authors.initials: I
authors.lastname: Ivanovic-Burmazovic
authors.forename: Milos R
authors.initials: MR
authors.lastname: Filipovic
authors.forename: Biljana
authors.initials: B
authors.lastname: Buzadzic
authors.forename: Ana
authors.initials: A
authors.lastname: Stancic
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Jankovic
authors.forename: Ksenija
authors.initials: K
authors.lastname: Velickovic
authors.forename: Igor
authors.initials: I
authors.lastname: Golic
authors.forename: Milica
authors.initials: M
authors.lastname: Markelic
authors.forename: Bato
authors.initials: B
authors.lastname: Korac
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D008297:Male; D008345:Manganese / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D018716:Molecular Mimicry; D009942:Organometallic Compounds / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D017931:DNA Primers; D009942:Organometallic Compounds; C121876:imisopasem manganese; D008345:Manganese; D013482:Superoxide Dismutase