title: Stem cell-based photodynamic therapy.
abstract: We have transfected murine neural stem cells (NSCs) and rat umbilical cord matrix-derived stem cells (RUCMSCs) with a plasmid expressing gaussia luciferase (gLuc). These cells are engineered to secrete the luciferase. We have used gLuc containing supernatant from culturing the NSCs to perform in vitro photodynamic therapy of murine melanoma cells (B16F10), and RUCMSCs to perform in vivo PDT of lung melanomas in C57BL/6 mice. The treatment system was comprised of aminolevulic acid as a prodrug for the synthesis of the photosensitizer protoporphyrin IX, gaussia luciferase, and its' substrate coelenterazine. A significant reduction of the number of live melanoma cells in vitro and a borderline significant retardation of tumour growth in vivo was observed after coelenterazine-mediated PDT.
authors.affiliation: Kansas State University, Anatomy & Physiology, Manhattan, KS 66506, USA. tbs3@vet.k-state.edu
authors.forename: Tej B
authors.initials: TB
authors.lastname: Shrestha
authors.forename: Gwi M
authors.initials: GM
authors.lastname: Seo
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Basel
authors.forename: Mausam
authors.initials: M
authors.lastname: Kalita
authors.forename: Hongwang
authors.initials: H
authors.lastname: Wang
authors.forename: David
authors.initials: D
authors.lastname: Villanueva
authors.forename: Marla
authors.initials: M
authors.lastname: Pyle
authors.forename: Sivasai
authors.initials: S
authors.lastname: Balivada
authors.forename: Raja Shekar
authors.initials: RS
authors.lastname: Rachakatla
authors.forename: Heather
authors.initials: H
authors.lastname: Shinogle
authors.forename: Prem S
authors.initials: PS
authors.lastname: Thapa
authors.forename: David
authors.initials: D
authors.lastname: Moore
authors.forename: Deryl L
authors.initials: DL
authors.lastname: Troyer
authors.forename: Stefan H
authors.initials: SH
authors.lastname: Bossmann
mesh_terms: D000622:Aminolevulinic Acid / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D007093:Imidazoles / Q000737:chemistry / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy; D008545:Melanoma / Q000188:drug therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D010084:Oxidation-Reduction; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity; D010957:Plasmids / Q000378:metabolism; D011524:Protoporphyrins / Q000096:biosynthesis / Q000627:therapeutic use / Q000633:toxicity; D011719:Pyrazines / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014162:Transfection
chemical_list: D007093:Imidazoles; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D011719:Pyrazines; C017144:coelenterazine; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX; D008156:Luciferases