title: Quantitative analysis of topoisomerase II alpha and evaluation of its effects on cell proliferation and apoptosis in glioblastoma cancer stem cells.
abstract: DNA topoisomerase II alpha (Topo-IIα) is an essential nuclear enzyme with its up-regulation demonstrated in different tumors. This study is to evaluate the expression of Topo-IIα in glioblastoma cancer stem cells (CSCs) and its effects on cell proliferation and apoptosis. We observed the expression differences of Topo-IIα gene transcript in CSCs and non-CSCs in glioblastoma cell line U87 by quantitative real-time PCR and western blot; we silenced Topo-IIα expression in U87 CSCs using Topo-IIα-specific siRNA to investigate the role of Topo-IIα in cell proliferation, cell cycle, and apoptosis. We found higher expression of Topo-IIα in CSCs than in non-CSCs. After using Topo-IIα-specific siRNA to silence the expression of Topo-IIα gene, we observed decreasing cell proliferation, cell cycle arrest, and induction of apoptosis in CSCs, which suggest that Topo-IIα contributes to both cell proliferation and/or cell survival. Topo-IIα may be a novel marker of cell proliferation and/or survival factor in glioblastoma CSCs. It may be a potential target that will open up new opportunities to develop therapeutic strategies for gliomas.
authors.affiliation: Department of Neurosurgery, Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
authors.forename: Meng
authors.initials: M
authors.lastname: Sang
authors.forename: Chao
authors.initials: C
authors.lastname: Shang
authors.forename: Yi-xue
authors.initials: YX
authors.lastname: Xue
authors.forename: Yun-hui
authors.initials: YH
authors.lastname: Liu
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004250:DNA Topoisomerases, Type II / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D005434:Flow Cytometry; D005909:Glioblastoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D006801:Humans; D014411:Neoplastic Stem Cells / Q000201:enzymology* / Q000473:pathology; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; D004250:DNA Topoisomerases, Type II