title: Expression and correlation of Bcl-2 with pathological grades in human             glioma stem cells.
abstract: The anti-apoptotic gene, B-cell lymphoma-2 (Bcl-2), has been reported to             be overexpressed in gliomas and is related to tumor prognosis, suggesting a potential             therapeutic target. Additionally, recent studies have demonstrated the existence             of brain glioma stem cells (BGSCs) which are tumorigenic, self-renewable and dominate             the biological behavior of gliomas. Currently BGSCs are committed as a new target             of glioma therapies. However, few studies have focused on the expression of Bcl-2             in BGSCs. We performed a series of experiments to culture BGSCs from eight clinical             specimens, followed by real-time RT-PCR and immunoassays to compare the expression             levels of Bcl-2 in BGSCs and their corresponding primary glioma cells (PGCs).             The results showed that Bcl-2 mRNA and protein expression levels are higher in             BGSCs compared to their counterparts, and the expression levels are related to             glioma malignancies. As an anti-apoptotic gene, Bcl-2 assigns immortality characteristics             to cells, which coincide with the pivotal biological feature of BGSCs. The experimental             results indicated that BGSCs would evade apoptosis for higher Bcl-2 expression,             and may interpret the drug resistance of glioma to cytotoxic drugs and other pro-apoptotic             agents. New therapies targeting Bcl-2 must induce apoptosis in BGSCs, thus, resulting             in treatment or even eradication of glioma.
authors.affiliation: Department of Neurosurgery, First Hospital of China Medical University, Heping District, Shenyang, Liaoning 110001, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qiu
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Tao
authors.forename: Yunjie
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D018874:Spheroids, Cellular / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D010455:Peptides; D019253:Proto-Oncogene Proteins c-bcl-2