title: Influence of 825 C>T polymorphism of G protein β3 subunit gene (GNB3) on hemodynamic response during dobutamine stress echocardiography.
abstract: There is a wide interpersonal difference to dobutamine response during dobutamine stress echocardiography (DSE). The aim of this study was to determine an association between GNB3 825C>T gene polymorphism, encoding the β3-subunit of G protein, and heart rate (HR), systolic (SBP) and diastolic blood pressure (DBP) response to dobutamine during DSE. The study involved 119 patients with clinical indications for DSE. Genotyping of GNB3 825C>T (rs5443) polymorphism was based on PCR-RFLP method with BseDI restriction enzyme. Significantly higher levels of both resting SBP and DBP in 825T allele carriers vs. 825CC patients were revealed. HR of 825CC vs. CT + TT subjects was slower along the test period reaching marked difference at dobutamine level 30 μg/kg/min (109 ± 20 vs. 116 ± 18 bpm, respectively, p = 0.047). SBP and DBP were markedly lower in 825CC homozygotes compared to 825T allele carriers throughout DSE. It can be concluded that GNB3 825C>T polymorphism is associated with resting SBP and DBP in Polish Caucasian patients subjected for diagnostic DSE. The polymorphism also modulate HR, SBP and DBP response during DSE.
authors.affiliation: Department of Experimental and Clinical Pharmacology, Pomeranian Medical University, Powstańcow Wlkp. 72, PL 70-111, Szczecin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Banaś
authors.forename: Edyta
authors.initials: E
authors.lastname: Płońska
authors.forename: Barbara
authors.initials: B
authors.lastname: Larysz
authors.forename: Mateusz
authors.initials: M
authors.lastname: Kurzawski
authors.forename: Marek
authors.initials: M
authors.lastname: Droździk
mesh_terms: D000483:Alleles; D001794:Blood Pressure / Q000235:genetics; D003971:Diastole / Q000235:genetics; D004280:Dobutamine; D025401:Echocardiography, Stress / Q000379:methods; D004453:Echoencephalography / Q000379:methods; D005260:Female; D006339:Heart Rate / Q000235:genetics; D006439:Hemodynamics / Q000235:genetics*; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D013599:Systole / Q000235:genetics
chemical_list: C454420:G-protein beta3 subunit; D004280:Dobutamine; D020962:Heterotrimeric GTP-Binding Proteins