title: A Reporter System for Translational Readthrough of Stop Codons in Human Cells.
abstract: Agents to induce readthrough of premature termination codons (PTCs) are useful research tools and potential therapeutics. Reporters used to detect PTC readthrough are gene-specific and thus are not suited to for general assessment of readthrough activity or in cases where PTC-inactivated genes are unknown. Here we describe a GFP-based reporter construct pMHG-W57* which is capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry. pMHG-W57* may be used as a general indicator of PTC readthrough in living cells and obviates the need for gene-specific recoding sequences in reporter constructs.
authors.affiliation: Department of Biochemistry, Vanderbilt University School of Medicine, Nashville TN 37232-6350, USA.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Halvey
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Liebler
authors.forename: Robbert J C
authors.initials: RJ
authors.lastname: Slebos