title: Chemoselective sequential "click" ligation using unsymmetrical bisazides.
abstract: Unsymmetrical bisazides containing chelating and nonchelating azido groups undergo chemoselective three-component copper(I)-catalyzed azide-alkyne conjugation reactions with two different alkyne molecules. In conjunction with the reactivity gap between aromatic and aliphatic alkynes, a bistriazole molecule can be generated with excellent regioselectivity by mixing two alkynes and a bisazide in a single reaction container. This method is applicable in aqueous solutions at neutral pH, which may lend utilities in bioconjugation applications.
authors.affiliation: Department of Chemistry and Biochemistry, Florida State University , Tallahassee, Florida 32306-4390, United States.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yuan
authors.forename: Gui-Chao
authors.initials: GC
authors.lastname: Kuang
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Clark
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu