title: Kinetics of hydrogen peroxide decomposition by catalase: hydroxylic solvent effects.
abstract: The effect of water-alcohol (methanol, ethanol, propan-1-ol, propan-2-ol, ethane-1,2-diol and propane-1,2,3-triol) binary mixtures on the kinetics of hydrogen peroxide decomposition in the presence of bovine liver catalase is investigated. In all solvents, the activity of catalase is smaller than in water. The results are discussed on the basis of a simple kinetic model. The kinetic constants for product formation through enzyme-substrate complex decomposition and for inactivation of catalase are estimated. The organic solvents are characterized by several physical properties: dielectric constant (D), hydrophobicity (log P), concentration of hydroxyl groups ([OH]), polarizability (α), Kamlet-Taft parameter (β) and Kosower parameter (Z). The relationships between the initial rate, kinetic constants and medium properties are analyzed by linear and multiple linear regression.
authors.affiliation: Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, Nr. 4-12, 030018, Bucharest, Romania. adina.raducan@g.unibuc.ro
authors.forename: Adina
authors.initials: A
authors.lastname: Raducan
authors.forename: Anca Ruxandra
authors.initials: AR
authors.lastname: Cantemir
authors.forename: Mihaela
authors.initials: M
authors.lastname: Puiu
authors.forename: Dumitru
authors.initials: D
authors.lastname: Oancea
mesh_terms: D000438:Alcohols / Q000737:chemistry*; D000818:Animals; D002374:Catalase / Q000737:chemistry*; D002417:Cattle; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D012997:Solvents / Q000737:chemistry*
chemical_list: D000438:Alcohols; D012997:Solvents; D006861:Hydrogen Peroxide; D002374:Catalase