title: Crystal structure of the lectin of Camptosema pedicellatum: implications of a conservative substitution at the hydrophobic subsite.
abstract: Lectins have been used as models for studies of the molecular basis of protein-carbohydrate interaction and specificity by deciphering codes present in the glycan structures. The purpose of the present study was to purify and solve the complete primary and crystal structure of the lectin of Camptosema pedicellatum (CPL) complexed with 5-bromo-4-chloro-3-indolyl-α-d-mannose (X-Man) using tandem mass spectrometry. CPL was purified by single-step affinity chromatography. Mass spectrometry findings revealed that purified CPL features a combination of chains weighing 25,298 ± 2 (α-chain), 12,835 ± 2 (β-chain) and 12,481 ± 2 Da (γ-chain). The solved crystal structure of CPL features a conservative mutation in the hydrophobic subsite, a constituent of the carbohydrate recognition domain (CRD), indicating the relevance of hydrophobic interactions in the establishment of interactions with carbohydrates. The substitution and the analysis of the interactions with X-Man also revealed that the hydrophobic effect caused by a minor change in the hydrophobic subsite interferes in the formation of H-bonds due to the reorientation of the indolyl group in the CRD.
authors.affiliation: BioMol-Lab, Laboratório de Moléculas Biologicamente Ativas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará 60440-970, Brazil.
authors.forename: Claudener
authors.initials: C
authors.lastname: Souza Teixeira
authors.forename: Helton Colares
authors.initials: HC
authors.lastname: da Silva
authors.forename: Tales Rocha
authors.initials: TR
authors.lastname: de Moura
authors.forename: Francisco N
authors.initials: FN
authors.lastname: Pereira-Júnior
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: do Nascimento
authors.forename: Celso Shiniti
authors.initials: CS
authors.lastname: Nagano
authors.forename: Alexandre Holanda
authors.initials: AH
authors.lastname: Sampaio
authors.forename: Plinio
authors.initials: P
authors.lastname: Delatorre
authors.forename: Bruno Anderson Matias
authors.initials: BA
authors.lastname: Rocha
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D007887:Fabaceae / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D007211:Indoles / Q000737:chemistry / Q000378:metabolism; D008358:Mannose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry* / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: C510192:5-bromo-4-chloro-3-indolylmannose; D007211:Indoles; D037121:Plant Lectins; D008358:Mannose