title: Heteroglycan from an alkaline extract of a somatic hybrid mushroom (PfloVv1aFB) of Pleurotus florida and Volvariella volvacea: structural characterization and study of immunoenhancing properties.
abstract: A water soluble heteroglycan, isolated from the alkaline extract of the fruit bodies of the somatic hybrid mushroom (PfloVv1aFB), raised through protoplast fusion between the strains of Pleorutus florida and Volverilla volvacea, was found to consist of (1→3)-, (1→6)-, (1→3,4)-linked, and terminal β-D-Glcp along with (1→2,6)-α-D-Galp and terminal α-D-Manp in a relative proportion of approximately 1:1:1:1:1:1. This polysaccharide exhibited strong immunostimulating activity of macrophages as well as splenocytes and thymocytes. Structural investigation was carried out using sugar analysis, methylation analysis; periodate oxidation study, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above mentioned experiments, the structure of the repeating unit of the polysaccharide was established as: [formula see text].
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Sanjoy Kumar
authors.initials: SK
authors.lastname: Bhunia
authors.forename: Biswajit
authors.initials: B
authors.lastname: Dey
authors.forename: Kankan K
authors.initials: KK
authors.lastname: Maity
authors.forename: Sukesh
authors.initials: S
authors.lastname: Patra
authors.forename: Soumitra
authors.initials: S
authors.lastname: Mandal
authors.forename: Swatilekha
authors.initials: S
authors.lastname: Maiti
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Samir R
authors.initials: SR
authors.lastname: Sikdar
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000468:Alkalies / Q000737:chemistry; D000818:Animals; D002236:Carbohydrate Conformation; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D020076:Pleurotus / Q000737:chemistry* / Q000276:immunology; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D017382:Reactive Oxygen Species / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013329:Structure-Activity Relationship; D060168:Thymocytes / Q000187:drug effects / Q000276:immunology; D055367:Volvariella / Q000737:chemistry* / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000468:Alkalies; D011134:Polysaccharides; D017382:Reactive Oxygen Species