title: Cardiotoxic effects of venom fractions from the Australian box jellyfish Chironex fleckeri on human myocardiocytes.
abstract: An investigation into the cardiotoxic effects in human cardiomyocytes of different fractions (as produced from an FPLC) of the venom from Chironex fleckeri showed that whole venom caused cardiac cell death in minutes, measured as cell detachment using xCELLigence technology. However, only one fraction of the venom was responsible for this effect. When all extracted venoms were recombined a similar result was seen for the toxic fraction, however these effects were slower than unfractionated venom alone even though the concentrations were similar. The difference in the results between fractioned and unfractionated venom may have been caused by compounds remaining in the FPLC column, which may interact with the toxic fraction to cause rapid cell detachment or death.
authors.affiliation: School of Marine and Tropical Biology, James Cook University, PO Box 6811, Cairns, QLD 4870, Australia.
authors.forename: Silvia L A
authors.initials: SL
authors.lastname: Saggiomo
authors.forename: Jamie E
authors.initials: JE
authors.lastname: Seymour
mesh_terms: D000818:Animals; D001315:Australia; D054715:Cardiotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003064:Cnidarian Venoms / Q000737:chemistry / Q000494:pharmacology*; D037462:Cubozoa / Q000378:metabolism*; D006801:Humans; D019156:Indian Ocean; D007700:Kinetics; D008387:Marine Toxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008970:Molecular Weight; D032383:Myocytes, Cardiac / Q000187:drug effects*; D058894:Nematocyst / Q000378:metabolism; D009994:Osmolar Concentration; D010140:Pacific Ocean; D015203:Reproducibility of Results
chemical_list: D054715:Cardiotoxins; D003064:Cnidarian Venoms; D008387:Marine Toxins