title: Oxidative stress status and DNA damage in saliva of human subjects with oral lichen planus and oral squamous cell carcinoma.
abstract: BACKGROUND
  The aim of this study was to evaluate oxidative stress status in the saliva of patients with oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  Thirty-two patients with OLP, 26 patients with OSCC, and 30 non-involved subjects were enrolled in this study. The study was conducted at the Cancer Department, Clinic of Oral Medicine, Tehran University of Medical Sciences. The unstimulated whole saliva malondialdehyde (MDA), as an indicator of lipid peroxidation, the total antioxidant capacity (TAC), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were assayed by thiobarbituric acid, ferric reducing antioxidant potential (FRAP), and ELISA method, respectively. The TAC/MDA ratio was used as an index of oxidative stress status. Data were analyzed by ANOVA followed by the Tukey's post hoc test.
  
  
  CONCLUSIONS
  There were no significant differences in saliva TAC and MDA levels between OLP and control, and also between OLP and OSCC patients. MDA and 8-OHdG were significantly higher but TAC was lower in OSCC patients than control. TAC/MDA ratio was significantly lower in patients with OSCC than both OLP patients and control. TAC/MDA ratio was significantly lower but 8-OHdG was higher in patients with OLP compared to control. This suggests that patients with OLP and OSCC are more susceptible to an imbalance of antioxidant-oxidative stress status.
authors.affiliation: Department of Oral Medicine, Dental Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. aghahose@sina.tums.ac.ir
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Agha-Hosseini
authors.forename: Iraj
authors.initials: I
authors.lastname: Mirzaii-Dizgah
authors.forename: Niloofar
authors.initials: N
authors.lastname: Farmanbar
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abdollahi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D004249:DNA Damage / Q000502:physiology; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D017676:Lichen Planus, Oral / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000502:physiology; D011230:Precancerous Conditions / Q000378:metabolism* / Q000473:pathology; D011237:Predictive Value of Tests; D012016:Reference Values; D018570:Risk Assessment; D012463:Saliva / Q000737:chemistry / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000378:metabolism; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D005609:Free Radicals; D012471:Salivary Proteins and Peptides; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine