title: Protective effect of sauchinone against regional myocardial ischemia/reperfusion injury: inhibition of p38 MAPK and JNK death signaling pathways.
abstract: Sauchinone has been known to have anti-inflammatory and antioxidant effects. We determined whether sauchinone is beneficial in regional myocardial ischemia/reperfusion (I/R) injury. Rats were subjected to 20 min occlusion of the left anterior descending coronary artery, followed by 2 hr reperfusion. Sauchinone (10 mg/kg) was administered intraperitoneally 30 min before the onset of ischemia. The infarct size was measured 2 hr after resuming the perfusion. The expression of cell death kinases (p38 and JNK) and reperfusion injury salvage kinases (phosphatidylinositol-3-OH kinases-Akt, extra-cellular signal-regulated kinases [ERK1/2])/glycogen synthase kinase (GSK)-3β was determined 5 min after resuming the perfusion. Sauchinone significantly reduced the infarct size (29.0% ± 5.3% in the sauchinone group vs 44.4% ± 6.1% in the control, P < 0.05). Accordingly, the phosphorylation of JNK and p38 was significantly attenuated, while that of ERK1/2, Akt and GSK-3β was not affected. It is suggested that sauchinone protects against regional myocardial I/R injury through inhibition of phosphorylation of p38 and JNK death signaling pathways.
authors.affiliation: Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Seok Jai
authors.initials: SJ
authors.lastname: Kim
authors.forename: Cheol Won
authors.initials: CW
authors.lastname: Jeong
authors.forename: Hong Beom
authors.initials: HB
authors.lastname: Bae
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Kwak
authors.forename: Jong-Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Chang-Seob
authors.initials: CS
authors.lastname: Seo
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: JongUn
authors.initials: J
authors.lastname: Lee
authors.forename: Kyung Yeon
authors.initials: KY
authors.lastname: Yoo
mesh_terms: D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D004149:Dioxoles / Q000494:pharmacology*; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D010766:Phosphorylation; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D001578:Benzopyrans; D004149:Dioxoles; D020011:Protective Agents; C410958:sauchinone; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D048031:JNK Mitogen-Activated Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048051:p38 Mitogen-Activated Protein Kinases; D038362:Glycogen Synthase Kinase 3