title: PDE4 control on cAMP/PKA compartmentation revealed by biosensor imaging in neurons.
abstract: Electrophysiological recordings (using the slow-AHP potassium current) together with novel biosensor imaging methods (with AKAR and Epac sensors) were used in preparations of rodent brain slices to record PKA activation in real time and in individual neurons. The experiments revealed the propagation of the PKA signal from the membrane to the cytosol and eventually to the nucleus. The experiments show how the geometry of the neurons combined with phosphodiesterase activities (mostly rolipram-sensitive PDE4) contributes to a functional compartmentation of the cAMP in subcellular domains.
authors.affiliation: Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France. pierre.vincent@upmc.fr
authors.forename: P
authors.initials: P
authors.lastname: Vincent
authors.forename: L R V
authors.initials: LR
authors.lastname: Castro
authors.forename: N
authors.initials: N
authors.lastname: Gervasi
authors.forename: E
authors.initials: E
authors.lastname: Guiot
authors.forename: M
authors.initials: M
authors.lastname: Brito
authors.forename: D
authors.initials: D
authors.lastname: Paupardin-Tritsch
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002451:Cell Compartmentation / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4 / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D012377:Rodentia; D020889:Rolipram / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4; D020889:Rolipram