title: Assays of PCB congeners and organochlorine insecticides with the transgenic Arabidopsis and tobacco plants carrying recombinant guinea pig AhR and GUS reporter genes.
abstract: Certain congeners of polychlorinated biphenyls (PCBs) and organochlorine insecticides are ligands of aryl hydrocarbon receptors (AhRs) in animals. A recombinant guinea pig (g) AhR, XgDV, was constructed by fusing the ligand-binding domain of gAhR, the DNA-binding domain of LexA, and the transactivating domain of VP16. Then, the expression unit of β-glucuronidase (GUS) reporter gene regulated by XgDV was introduced into Arabidopsis and tobacco plants. When the transgenic Arabidopsis XgDV plants were cultured on Murashige-Skoog (MS) medium containing PCB congeners, the GUS activity in the plants increased toxic equivalent (TEQ)-dependently. The GUS activity in the transgenic Arabidopsis XgDV plants cultured on MS medium containing the organochlorine insecticide dieldrin was also induced. On the other hand, in the case of DDT, the GUS activity induced by 3-methylcholanthere in the plants decreased. The transgenic Arabidopsis XgDV plants detected 1000 ng g(-1) PCB126 in 1 g of soils. Thus the XgDV plants seemed to be useful for convenient assays of PCB congeners and organochlorine insecticides, without any extraction and purification steps.
authors.affiliation: Research Center for Environmental Genomics, Kobe University, Kita-ku, Kobe, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Gion
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Inui
authors.forename: Hideaki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Utani
authors.forename: Susumu
authors.initials: S
authors.lastname: Kodama
authors.forename: Hideo
authors.initials: H
authors.lastname: Ohkawa
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D001681:Biological Assay / Q000379:methods*; D004784:Environmental Monitoring; D015870:Gene Expression; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D006168:Guinea Pigs / Q000235:genetics; D006843:Hydrocarbons, Chlorinated / Q000737:chemistry / Q000494:pharmacology*; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000737:chemistry*; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics* / Q000378:metabolism; D013237:Stereoisomerism; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D018336:Receptors, Aryl Hydrocarbon; D011078:Polychlorinated Biphenyls; D005966:Glucuronidase