title: Expression of osmotic stress protein 94 in murine endolymphatic hydrops model.
abstract: CONCLUSIONS
  The up-regulation of osmotic stress protein 94 (OSP94) in the murine endolymphatic hydrops (EH) models suggests that OSP94 might be involved in cellular adaptation in response to ionic and osmotic stress in the murine inner ear.
  
  
  OBJECTIVE
  The purpose of the present study was to investigate the expression of OSP94 in cochlear tissues of the murine EH models and control animals.
  
  
  METHODS
  Nine adult BALB/c mice were treated with both intratympanic injection of lipopolysaccharide and intraperitoneal administration of aldosterone to induce EH. Nine mice were used as control animals. The expression level of OSP94 in the EH and control groups was compared using immunohistochemistry and real-time RT-PCR.
  
  
  RESULTS
  Immunohistochemical staining of tissues in the EH group showed an up-regulation of OSP94 expression in the cochlea, especially in the stria vascularis and Reissner's membrane. Quantitative real-time PCR analysis also showed that transcription of the OSP94 gene in the cochlea was significantly up-regulated in the EH group.
authors.affiliation: Department of Physiology, Seoul National University, College of Medicine, Seoul, Korea.
authors.forename: Chang-Hee
authors.initials: CH
authors.lastname: Kim
authors.forename: Young Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Byung Yoon
authors.initials: BY
authors.lastname: Choi
authors.forename: Ho Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Seung-Ha
authors.initials: SH
authors.lastname: Oh
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D003053:Cochlear Duct; D018159:Endolymphatic Hydrops / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D007150:Immunohistochemistry; D051379:Mice; D012313:RNA / Q000096:biosynthesis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012313:RNA; C099678:Hspa4l protein, mouse