title: Synthetic peptide arrays for investigating protein interaction domains.
abstract: Synthetic peptide array technology was first developed in the early 1990s by Ronald Frank. Since then the technique has become a powerful tool for high throughput approaches in biology and biochemistry. Here, we focus on peptide arrays applied to investigate the binding specificity of protein interaction domains such as WW, SH3, and PDZ domains. We describe array-based methods used to reveal domain networks in yeast, and briefly review rules as well as ideas about the synthesis and application of peptide arrays. We also provide initial results of a study designed to investigate the nature and evolution of SH3 domain interaction networks in eukaryotes.
authors.affiliation: Institut für Medizinische Immunologie Berlin, Molecular Libraries and Recognition Group, Charité-Universitätsmedizin Berlin, Hessische Str. 3-4, 10115 Berlin, Germany. rve@charite.de
authors.forename: Rudolf
authors.initials: R
authors.lastname: Volkmer
authors.forename: Victor
authors.initials: V
authors.lastname: Tapia
authors.forename: Christiane
authors.initials: C
authors.lastname: Landgraf
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000379:methods; D002482:Cellulose / Q000737:chemistry; D004867:Equipment Design; D005656:Fungal Proteins / Q000737:chemistry; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping / Q000379:methods*; D017434:Protein Structure, Tertiary
chemical_list: D005656:Fungal Proteins; D019151:Peptide Library; D010455:Peptides; D002482:Cellulose