title: Detection of apoptosis by TUNEL assay.
abstract: Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.
authors.forename: Kateryna
authors.initials: K
authors.lastname: Kyrylkova
authors.forename: Sergiy
authors.initials: S
authors.lastname: Kyryachenko
authors.forename: Mark
authors.initials: M
authors.lastname: Leid
authors.forename: Chrissa
authors.initials: C
authors.lastname: Kioussi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D004622:Embryo, Mammalian / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections / Q000379:methods*; D020287:In Situ Nick-End Labeling / Q000379:methods*; D051379:Mice