title: Expression of genes for interleukins, neuropeptides, growth hormone receptor, and leptin receptor in adipose tissue from growing broiler chickens.
abstract: In this study, total RNA was collected from abdominal adipose tissue samples obtained from 10 broiler chickens at 3, 4, 5, and 6 wk of age and prepared for quantitative real-time PCR analysis. Quantitative real-time PCR analysis was used to examine the influence of age on the expression of the adipose tissue genes for IL-1β, -6, -10, -15, -18; brain-derived neurotropic factor; ciliary neurotropic factor; interferon γ, neuropeptide Y receptor Y1; neuropeptide Y; nucleobindin 2; growth hormone receptor; leptin receptor; and visfatin. Between 3 and 6 wk of age, leptin receptor expression decreased (P=0.013) with age, whereas expression of IL-15 (P=0.015) and growth hormone receptor (P=0.002) increased. Furthermore, IL-18 (P<0.001) and visfatin (P=0.007) expression increased between 4 and 6 wk of age. This is a unique exhibition of age-related changes in cytokine gene expression in chicken adipose tissue. Future studies are needed to elucidate the role of adipose tissue cytokines in growth and, possibly, disease resistance.
authors.affiliation: Poultry Processing and Swine Physiology Research, Agricultural Research Service, USDA, Athens, GA 30605, USA. gary.hausman@ars.usda.gov
authors.forename: G J
authors.initials: GJ
authors.lastname: Hausman
authors.forename: C R
authors.initials: CR
authors.lastname: Barb
authors.forename: B D
authors.initials: BD
authors.lastname: Fairchild
authors.forename: J
authors.initials: J
authors.lastname: Gamble
authors.forename: L
authors.initials: L
authors.lastname: Lee-Rutherford
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002645:Chickens / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D054411:Receptors, Leptin / Q000235:genetics / Q000378:metabolism*; D011986:Receptors, Somatotropin / Q000235:genetics / Q000378:metabolism*
chemical_list: D007378:Interleukins; D009479:Neuropeptides; D054411:Receptors, Leptin; D011986:Receptors, Somatotropin; D012313:RNA