title: Sensitive, accurate and simple liquid chromatography-tandem mass spectrometric method for the quantitation of amphotericin B in human or minipig plasma.
abstract: Amphotericin B (AMB) is still the standard care for systemic fungal infections. This paper describes a sensitive, accurate and simple liquid chromatography-tandem mass spectrometry method to quantify AMB in human or minipig plasma. Samples were prepared through protein precipitation by adding methanol-acetonitrile (1:3, v/v) to either human or minipig plasma. High-performance liquid chromatography separation was conducted on a 10-cm Gemini C18 column with a 7-min gradient of mobile phase comprised of buffer A (0.1% formic acid aqueous solution) and buffer B (methanol-acetonitrile, 2:3, v/v). AMB was detected through multiple reaction monitoring (MRM) with a mass transition of 924.60 → 743.30 and the internal standard paclitaxel was detected through MRM with a mass transition of 854.30 → 286.10. The method had a linear range between 5 and 2500 ng/mL with lower limit of quantitation of 3 ng/mL. The overall recovery was 113 ± 4.06% in human plasma and 94.8 ± 7.38% in minipig plasma. The method has been validated and applied for AMB pharmacokinetic study in both human and minipig plasma.
authors.affiliation: Analytical Pharmacology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.
authors.forename: WeiGe
authors.initials: W
authors.lastname: Qin
authors.forename: Hui
authors.initials: H
authors.lastname: Tao
authors.forename: Yoryu
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhaoyuan
authors.initials: Z
authors.lastname: Chen
authors.forename: Nian
authors.initials: N
authors.lastname: Wu
mesh_terms: D000666:Amphotericin B / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D013552:Swine; D013556:Swine, Miniature; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000666:Amphotericin B