title: Vector-based miR-15a/16-1 plasmid inhibits colon cancer growth in vivo.
abstract: miR-15 (microRNA 15) and miR-16 are frequently deleted or down-regulated in many cancer cell lines and various tumour tissues, suggesting that miR-15a/16-1 plays important roles in tumour progression and might be a method for cancer treatment. We have developed a vector-based plasmid to explore the anti-tumour efficacy of miR-15a/16-1 in colon cancer in vivo. It is proposed that miR-15a and miR-16-1 target cyclin B1 (CCNB1), which associates with several tumorigenic features such as survival and proliferation. The levels of miR-15a and miR-16-1 in colon cancer cells were inversely correlated with CCNB1 expression, and there was consensus between miR-15a/16-1 and CCNB1 mRNA sequences by analysing homology. Vector-based miR-15a/16-1 expression plasmid was constructed and transfected into HCT 116 and SW620 colon cancer cells in vitro. The effects produced on cell viability and angiogenesis were analysed using flow cytometric analysis, colony formation analysis and tube formation analysis. CCNB1 expression down-regulation was checked by Western blotting. Systemic delivery of miR-15a/16-1 plasmids encapsulated in cationic liposome led to a significant inhibition of subcutaneous tumour growth and angiogenesis in tumour tissues, whereas no effects were observed with liposome carrying the non-specific plasmid. In summary, miR-15a/16-1 has been applied in colon cancer treatment in vivo, and resulted in effective colon tumour xenografts growth arrest and angiogenesis decrease. These findings suggest that systemic delivery of vector-based miR-15a/16-1 expression plasmid can be an approach to colon cancer therapy.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Dai
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Jiang
authors.forename: Ruibo
authors.initials: R
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Dai
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Wei
authors.forename: Hong-Xin
authors.initials: HX
authors.lastname: Deng
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D056744:Cyclin B1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D045325:HCT116 Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D014183:Transplantation, Heterologous
chemical_list: D020413:3' Untranslated Regions; C534699:CCNB1 protein, human; D056744:Cyclin B1; C505361:MIRN15 microRNA, human; C505362:MIRN16 microRNA, human; D035683:MicroRNAs