title: High spatial and temporal resolution wide-field imaging of neuron activity using quantum NV-diamond.
abstract: A quantitative understanding of the dynamics of biological neural networks is fundamental to gaining insight into information processing in the brain. While techniques exist to measure spatial or temporal properties of these networks, it remains a significant challenge to resolve the neural dynamics with subcellular spatial resolution. In this work we consider a fundamentally new form of wide-field imaging for neuronal networks based on the nanoscale magnetic field sensing properties of optically active spins in a diamond substrate. We analyse the sensitivity of the system to the magnetic field generated by an axon transmembrane potential and confirm these predictions experimentally using electronically-generated neuron signals. By numerical simulation of the time dependent transmembrane potential of a morphologically reconstructed hippocampal CA1 pyramidal neuron, we show that the imaging system is capable of imaging planar neuron activity non-invasively at millisecond temporal resolution and micron spatial resolution over wide-fields.
authors.affiliation: School of Physics, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: L T
authors.initials: LT
authors.lastname: Hall
authors.forename: G C G
authors.initials: GC
authors.lastname: Beart
authors.forename: E A
authors.initials: EA
authors.lastname: Thomas
authors.forename: D A
authors.initials: DA
authors.lastname: Simpson
authors.forename: L P
authors.initials: LP
authors.lastname: McGuinness
authors.forename: J H
authors.initials: JH
authors.lastname: Cole
authors.forename: J H
authors.initials: JH
authors.lastname: Manton
authors.forename: R E
authors.initials: RE
authors.lastname: Scholten
authors.forename: F
authors.initials: F
authors.lastname: Jelezko
authors.forename: Jörg
authors.initials: J
authors.lastname: Wrachtrup
authors.forename: S
authors.initials: S
authors.lastname: Petrou
authors.forename: L C L
authors.initials: LC
authors.lastname: Hollenberg
mesh_terms: D000465:Algorithms; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D001921:Brain / Q000502:physiology*; D001931:Brain Mapping / Q000379:methods*; D056547:CA1 Region, Hippocampal / Q000502:physiology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D060526:Magnetic Fields; D008959:Models, Neurological; D036103:Nanotechnology / Q000379:methods; D009415:Nerve Net / Q000502:physiology; D009474:Neurons / Q000502:physiology*