title: Nitroxide sensing of a DNA microenvironment: mechanistic insights from EPR spectroscopy and molecular dynamics simulations.
abstract: The behavior of the nitroxide spin labels 1-oxyl-4-bromo-2,2,5,5-tetramethylpyrroline (R5a) and 1-oxyl-2,2,5,5-tetramethylpyrroline (R5) attached at a phosphorothioate-substituted site in a DNA duplex is modulated by the DNA in a site- and stereospecific manner. A better understanding of the mechanisms of R5a/R5 sensing of the DNA microenvironment will enhance our capability to relate information from nitroxide spectra to sequence-dependent properties of DNA. Toward this goal, electron paramagnetic resonance (EPR) spectroscopy and molecular dynamics (MD) simulations were used to investigate R5 and R5a attached as R(p) and S(p) diastereomers at phosphorothioate (pS)C(7) of d(CTACTG(pS)C(7)Y(8)TTAG). d(CTAAAGCAGTAG) (Y = T or U). X-band continuous-wave EPR spectra revealed that the dT(8) to dU(8) change alters nanosecond rotational motions of R(p)-R5a but produces no detectable differences for S(p)-R5a, R(p)-R5, and S(p)-R5. MD simulations were able to qualitatively account for these spectral variations and provide a plausible physical basis for the R5/R5a behavior. The simulations also revealed a correlation between DNA backbone B(I)/B(II) conformations and R5/R5a rotational diffusion, thus suggesting a direct connection between DNA local backbone dynamics and EPR-detectable R5/R5a motion. These results advance our understanding of how a DNA microenvironment influences nitroxide motion and the observed EPR spectra. This may enable use of R5/R5a for a quantitative description of the sequence-dependent properties of large biologically relevant DNA molecules.
authors.affiliation: Department of Chemistry, University of Southern California, Los Angeles, California 90089-0744, United States.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Popova
authors.forename: Ma'mon M
authors.initials: MM
authors.lastname: Hatmal
authors.forename: Maria P
authors.initials: MP
authors.lastname: Frushicheva
authors.forename: Eric A
authors.initials: EA
authors.lastname: Price
authors.forename: Peter Z
authors.initials: PZ
authors.lastname: Qin
authors.forename: Ian S
authors.initials: IS
authors.lastname: Haworth
mesh_terms: D004247:DNA / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D056004:Molecular Dynamics Simulation; D009589:Nitrogen Oxides / Q000737:chemistry*
chemical_list: D009589:Nitrogen Oxides; D004247:DNA