title: Long working distance fluorescence lifetime imaging with stimulated emission and electronic time delay.
abstract: In this work, long working distance fluorescence lifetime imaging is realized with stimulated emission in combination with electronic time delay control. Spatial coherence, as a result of stimulated emission, supports unattenuated fluorescence detection at extended distance, using low NA optics. An electronic time delayed trigger provides an advantageous way in adjusting the pulse separation and probing the fluorescence lifetime in the nanosecond ranges. The fluorescence lifetime of selected fluorophores is accurately determined through the pump-probe configuration. The characteristics and applications in fluorescence lifetime measurement of stimulated emission are investigated and summarized succinctly here.
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Po-Yen
authors.initials: PY
authors.lastname: Lin
authors.forename: Shin-Shian
authors.initials: SS
authors.lastname: Lee
authors.forename: Chia-Seng
authors.initials: CS
authors.lastname: Chang
authors.forename: Fu-Jen
authors.initials: FJ
authors.lastname: Kao
mesh_terms: D003198:Computer Simulation; D004581:Electronics; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008027:Light; D055095:Optics and Photonics; D010777:Photochemistry / Q000379:methods; D012666:Semiconductors; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes