title: Adrenodoxin--a versatile ferredoxin.
abstract: Mammalian adrenodoxin (Adx) has been known for many years as an essential electron mediator in mitochondrial cytochrome P450 systems. Because of its ability to support several cytochrome P450 enzymes, it is involved not only in adrenal steroid hormone biosynthesis but also in vitamin D and bile acid metabolism. Recently, Adx is increasingly gaining attention because of its potential for pharmaceutical industry and biotechnology. With human cytochromes P450 becoming important drug targets, suitable Adx-based screening systems have to be developed to test putative new drugs. Moreover, in artificial systems, Adx has been shown to functionally interact with diverse bacterial cytochromes P450 catalyzing a variety of chemically interesting reactions. Putative biotechnological applications of such Adx-containing reconstituted systems are discussed.
authors.affiliation: Department of Biochemistry, Saarland University, Saarbrücken, Germany.
authors.forename: Kerstin Maria
authors.initials: KM
authors.lastname: Ewen
authors.forename: Michael
authors.initials: M
authors.lastname: Ringle
authors.forename: Rita
authors.initials: R
authors.lastname: Bernhardt
mesh_terms: D000325:Adrenodoxin / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology*; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000502:physiology; D003067:Coenzymes / Q000096:biosynthesis; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000502:physiology; D004353:Drug Evaluation, Preclinical; D005288:Ferredoxins / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D008928:Mitochondria / Q000201:enzymology; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003067:Coenzymes; D005288:Ferredoxins; D011994:Recombinant Proteins; D000325:Adrenodoxin; D003577:Cytochrome P-450 Enzyme System