title: Protective effects of Lycium barbarum polysaccharide on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion.
abstract: This study investigated the protective effects of Lycium barbarum polysaccharide (LBP) on alleviating injury from oxygen-glucose deprivation/reperfusion (OGD/RP) in primary cultured rat hippocampal neurons. Cultured hippocampal neurons were exposed to oxygen-glucose deprivation (OGD) for 2 h followed by a 24 h re-oxygenation. The MTT assay and the lactate dehydrogenase (LDH) release were used to determine the neuron viability. Superoxide dismutase (SOD), Glutathione peroxidase (GSH-PX), malondialdehyde (MDA) were determined by spectrophotometry using commercial kits. Mitochondrial membrane potential (MMP) and the intracellular free calcium concentration ([Ca(2+)](i)) in hippocampal neurons were measured using the confocal laser scanning microscope (CLSM). Treatment with LBP (10-40 mg/l) significantly attenuated neuronal damage and inhibited LDH release in a dose-dependent manner. Furthermore, LBP enhanced activities of SOD and GSH-PX but it decreased their MDA content, inhibited [Ca(2+)](i) elevation and decrease of MMP in ischemia-reperfusion treated hippocampal neurons. These findings suggested that LBP may be a potential neuroprotective agent for cerebral ischemia-reperfusion injury.
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Rui
authors.forename: Li
authors.initials: L
authors.lastname: Yuxiang
authors.forename: Hao
authors.initials: H
authors.lastname: Yinju
authors.forename: Zhu
authors.initials: Z
authors.lastname: Qingluan
authors.forename: Wu
authors.initials: W
authors.lastname: Yang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Qipeng
authors.forename: Wang
authors.initials: W
authors.lastname: Hao
authors.forename: Ma
authors.initials: M
authors.lastname: Lin
authors.forename: Liu
authors.initials: L
authors.lastname: Juan
authors.forename: Zhao
authors.initials: Z
authors.lastname: Chengjun
authors.forename: Jiang
authors.initials: J
authors.lastname: Yuanxu
authors.forename: Wang
authors.initials: W
authors.lastname: Yanrong
authors.forename: Dai
authors.initials: D
authors.lastname: Xiuying
authors.forename: Zhang
authors.initials: Z
authors.lastname: Wannian
authors.forename: Sun
authors.initials: S
authors.lastname: Tao
authors.forename: Yu
authors.initials: Y
authors.lastname: Jianqiang
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005947:Glucose / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D020011:Protective Agents / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy*
chemical_list: D003470:Culture Media; D004365:Drugs, Chinese Herbal; D020011:Protective Agents; C500223:lycium barbarum polysaccharide; D005947:Glucose; D010100:Oxygen