title: Substrate spectrum extension of PenA in Burkholderia thailandensis with a single amino acid deletion, Glu168del.
abstract: We describe a deletion mutation in a class A β-lactamase, PenA, of Burkholderia thailandensis that extended the substrate spectrum of the enzyme to include ceftazidime. Glu168del was located in a functional domain called the omega loop causing expansion of the space in the loop, which in turn increased flexibility at the active site. This deletion mutation represents a rare but significant alternative mechanical path to substrate spectrum extension in PenA besides more common substitution mutations.
authors.affiliation: Department of Medicine, College of Medicine, Korea University, Anam-Dong, Seongbuk-Gu, Seoul, South Korea.
authors.forename: Hyojeong
authors.initials: H
authors.lastname: Yi
authors.forename: Karan
authors.initials: K
authors.lastname: Kim
authors.forename: Kwang-Hwi
authors.initials: KH
authors.lastname: Cho
authors.forename: Oksung
authors.initials: O
authors.lastname: Jung
authors.forename: Heenam Stanley
authors.initials: HS
authors.lastname: Kim
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019117:Burkholderia / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D002442:Ceftazidime / Q000494:pharmacology; D017384:Sequence Deletion / Q000235:genetics; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002442:Ceftazidime; D001618:beta-Lactamases