title: Solid-state fermentation: tool for bioremediation of adsorbed textile dyestuff on distillery industry waste-yeast biomass using isolated Bacillus cereus strain EBT1.
abstract: Bioremediation of textile dyestuffs under solid-state fermentation (SSF) using industrial wastes as substrate pose an economically feasible, promising, and eco-friendly alternative. The purpose of this study was to adsorb Red M5B dye, a sample of dyes mixture and a real textile effluent on distillery industry waste-yeast biomass (DIW-YB) and its further bioremediation using Bacillus cereus EBT1 under SSF. Textile dyestuffs were allowed to adsorb on DIW-YB. DIW-YB adsorbed dyestuffs were decolorized under SSF by using B. cereus. Enzyme analysis was carried out to ensure decolorization of Red M5B. Metabolites after dye degradation were analyzed using UV-Vis spectroscopy, FTIR, HPLC, and GC-MS. DIW-YB showed adsorption of Red M5B, dyes mixture and a textile wastewater sample up to 87, 70, and 81 %, respectively. DIW-YB adsorbed Red M5B was decolorized up to 98 % by B. cereus in 36 h. Whereas B. cereus could effectively reduce American Dye Manufacture Institute value from DIW-YB adsorbed mixture of textile dyes and textile wastewater up to 70 and 100 %, respectively. Induction of extracellular enzymes such as laccase and azoreductase suggests their involvement in dye degradation. Repeated utilization of DIW-YB showed consistent adsorption and ADMI removal from textile wastewater up to seven cycles. HPLC and FTIR analysis confirms the biodegradation of Red M5B. GC-MS analysis revealed the formation of new metabolites. B. cereus has potential to bioremediate adsorbed textile dyestuffs on DIW-YB. B. cereus along with DIW-YB showed enhanced decolorization performance in tray bioreactor which suggests its potential for large-scale treatment procedures.
authors.affiliation: Department of Biotechnology, Shivaji University, Kolhapur 416004, India.
authors.forename: Avinash A
authors.initials: AA
authors.lastname: Kadam
authors.forename: Jeevan D
authors.initials: JD
authors.lastname: Kamatkar
authors.forename: Rahul V
authors.initials: RV
authors.lastname: Khandare
authors.forename: Jyoti P
authors.initials: JP
authors.lastname: Jadhav
authors.forename: Sanjay P
authors.initials: SP
authors.lastname: Govindwar
mesh_terms: D000327:Adsorption; D001409:Bacillus cereus / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000378:metabolism* / Q000633:toxicity; D004798:Enzymes / Q000378:metabolism; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D012336:RNA, Ribosomal, 16S; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013783:Textile Industry; D062065:Wastewater; D015003:Yeasts / Q000378:metabolism
chemical_list: D004396:Coloring Agents; D004798:Enzymes; D007220:Industrial Waste; D009584:Nitrogen; D012336:RNA, Ribosomal, 16S; D062065:Wastewater