title: Purification of vetiver alcohols and esters for quantitative high-performance thin-layer chromatography determination in Haitian vetiver essential oils and vetiver acetates.
abstract: A simple, fast, and efficient High-Performance Thin-Layer Chromatography (HPTLC) method was developed for the simultaneous quantitative determination of alcohols and acetates in Haitian vetiver essential oils (Chryzopogon zizanioides) and its acetylated form. Analytes were separated by using a mixture of n-hexane-chloroform-ethyl acetate (8:6:0.5, v/v/v) as mobile phase under 47% humidity. Quantification was achieved by densitometric evaluation of the analytes in absorbance mode under visible light (λ=530 nm) after staining with a vanillin-sulfuric acid reagent. Reference mixtures of alcohols and acetates were obtained by fractionation of Haitian vetiver oil or vetiver acetates, followed by purification of the fractions of interest by means of Over-Pressured Layer Chromatography (OPLC). The chemical composition of each reference fraction was determined by using GC-MS and GC×GC-MS, and their overall purity was determined by GC/FID and HPTLC. The TLC method provided compact spots for alcohols (R(f2)=0.18±0.01 and R(f1)=0.28±0.01) and acetates (R(f3)=0.65±0.01). Calibration plots showed good linear correlation with r²=0.9995±0.0001 and r²=0.9995±0.0001 for alcohols and r²=0.9996±0.0001 for acetates in a 40-200 ng spot⁻¹ concentration range with respect to peak areas. The method was validated for precision and accuracy. Limit of detection (LOD) and quantification (LOQ) were determined. Method specificity was confirmed using retention factor (R(f)) and GC-MS control of the standards reference mixtures.
authors.affiliation: Charabot S.A., 10 Avenue Yves Emmanuel Baudoin, 06130 Grasse, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Paillat
authors.forename: Christine
authors.initials: C
authors.lastname: Périchet
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Pierrat
authors.forename: Sophie
authors.initials: S
authors.lastname: Lavoine
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Filippi
authors.forename: Uwe
authors.initials: U
authors.lastname: Meierhenrich
authors.forename: Xavier
authors.initials: X
authors.lastname: Fernandez
mesh_terms: D000085:Acetates; D000438:Alcohols / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer / Q000379:methods*; D004952:Esters / Q000032:analysis* / Q000302:isolation & purification; D006205:Haiti; D057230:Limit of Detection; D016014:Linear Models; D009822:Oils, Volatile / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D006109:Poaceae / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000085:Acetates; D000438:Alcohols; D004952:Esters; D009822:Oils, Volatile; D010936:Plant Extracts