title: Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5.
abstract: Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA-amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and determination of the affinity constant.
authors.affiliation: Département de Chimie Moléculaire, UMR CNRS 5250, Université Joseph Fourier, BP53, 38041 Grenoble cedex 9, France. hajra.basit@ujf-grenoble.fr angeline.van-der-heyden@ujf-grenoble.fr
authors.forename: Hajra
authors.initials: H
authors.lastname: Basit
authors.forename: K
authors.initials: K
authors.lastname: Shivaji Sharma
authors.forename: Angéline
authors.initials: A
authors.lastname: Van der Heyden
authors.forename: Chantal
authors.initials: C
authors.lastname: Gondran
authors.forename: Cécile
authors.initials: C
authors.lastname: Breyton
authors.forename: Pascal
authors.initials: P
authors.lastname: Dumy
authors.forename: Françoise M
authors.initials: FM
authors.lastname: Winnik
authors.forename: Pierre
authors.initials: P
authors.lastname: Labbé
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D001435:Bacteriophages / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism*; D013604:T-Phages / Q000737:chemistry* / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; D014764:Viral Proteins