title: Thymidylate synthase (TS) protein expression as a prognostic factor in advanced colorectal cancer: a comparison with TS mRNA expression.
abstract: OBJECTIVE
  The role of intratumoral thymidylate synthase (TS) mRNA or protein expression is still controversial and little has been reported regarding relation of them in colorectal cancer.
  
  
  METHODS
  Forty-six patients with advanced colorectal cancer who underwent surgical resection were included. TS mRNA expression was determined by the Danenberg tumor profile method based on laser-captured micro-dissection of the tumor cells. TS protein expression was evaluated using immunohistochemical staining.
  
  
  RESULTS
  TS mRNA expression tended to relate TS protein expression. Statistical significance was not found in overall survival between the TS mRNA high group and low group regardless of performing adjuvant chemotherapy. The overall survival in the TS protein negative group was significantly higher than that in positive group in all and the patients without adjuvant chemotherapy. Multivariate analysis showed TS protein expression was as an independent prognostic factor.
  
  
  CONCLUSIONS
  TS protein expression tends to be related TS mRNA expression and is an independent prognostic factor in advanced colorectal cancer.
authors.affiliation: Department of Surgery, the University of Tokushima, Tokushima, Japan.
authors.forename: Tateo
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Shimada
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Kurita
authors.forename: Takashi
authors.initials: T
authors.lastname: Iwata
authors.forename: Masanori
authors.initials: M
authors.lastname: Nishioka
authors.forename: Kozo
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Jun
authors.initials: J
authors.lastname: Higashijima
authors.forename: Tohru
authors.initials: T
authors.lastname: Utsunomiya
mesh_terms: D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D013940:Thymidylate Synthase / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D013940:Thymidylate Synthase