title: Taraxerone enhances alcohol oxidation via increases of alcohol dehyderogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities and gene expressions.
abstract: The present study, taraxerone (d-friedoolean-14-en-3-one) was isolated from Sedum sarmentosum with purity 96.383%, and its enhancing effects on alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities were determined: EC(50) values were 512.42 ± 3.12 and 500.16 ± 3.23 μM for ADH and ALDH, respectively. In order to obtain more information on taraxerone related with the alcohol metabolism, 40% ethanol (5 mL/kg body weight) with 0.5-1mM of taraxerone were administered to mice. The plasma alcohol and acetaldehyde concentrations of taraxerone-treated groups were significantly lowered than those of the control group (p<0.01): approximately 20-67% and 7-57% lowered for plasma alcohol and acetaldehyde, respectively. Compare to the control group, the ADH and ALDH expressions in the liver tissues were abruptly increased in the taraxerone-treated groups after ethanol exposure. In addition, taraxerone prevented catalase, superoxide dismutase, and reduced glutathione concentrations from the decrease induced by ethanol administration with the concentration dependent manner.
authors.affiliation: Department of Food Science and Technology, Chungnam National University, 305-764 Daejeon, Republic of Korea.
authors.forename: Chang-Keun
authors.initials: CK
authors.lastname: Sung
authors.forename: Seung-Mi
authors.initials: SM
authors.lastname: Kim
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Oh
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Yang
authors.forename: Byung-Hee
authors.initials: BH
authors.lastname: Han
authors.forename: Eun-Kyoung
authors.initials: EK
authors.lastname: Mo
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D010084:Oxidation-Reduction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000438:Alcohols; D017931:DNA Primers; D009828:Oleanolic Acid; D000426:Alcohol Dehydrogenase; D000445:Aldehyde Oxidoreductases; C018699:aldehyde dehydrogenase (NAD(P)+); C009379:taraxerone