title: Investigation on BRCA1 SNPs and its effects on mastitis in Chinese commercial cattle.
abstract: The main objective of this study was to investigate whether the bovine breast cancer 1 (BRCA1) gene was associated with mastitis resistance in Chinese commercial cattle. A total of 51 SNPs were screened from public data resources and DNA sequencing. Three SNPs (c.5682G>C,c.26198C>T and c.46126G>T) were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and created restriction site PCR (CRS-PCR) methods and 21 combinations of these SNPs were observed. The single SNP and their genetic effects on somatic cell score (SCS) were evaluated and a significant association with SCS was found in c.46126G>T. The mean SCS of individuals with genotype KK was significantly lower than those of genotypes KL and LL. The results of combined genotypes analysis of three SNPs showed that HHLLNN genotype with the highest SCS was easily for the mastitis susceptibility, whereas GGKKMM genotype with the lowest SCS was favorable for the mastitis resistance. The information provided in the present study will be very useful for improving mastitis resistance in dairy cattle by marker-assisted selection (MAS).
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, People's Republic of China. zhengrong_yuan@yahoo.com.cn
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Yuan
authors.forename: Junya
authors.initials: J
authors.lastname: Li
authors.forename: Jiao
authors.initials: J
authors.lastname: Li
authors.forename: Lupei
authors.initials: L
authors.lastname: Zhang
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Hui Jiang
authors.initials: HJ
authors.lastname: Gao
authors.forename: Shangzhong
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D019313:BRCA1 Protein / Q000235:genetics*; D002417:Cattle; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D008414:Mastitis, Bovine / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D019313:BRCA1 Protein