title: A self-ligation method for PCR-sequencing the telomeres of Streptomyces and Mycobacterium linear replicons.
abstract: Actinomycete species from many genera often harbor linear plasmids and some contain linear chromosomes. A self-ligation and PCR-sequencing method was developed for identifying three novel telomere sequences of linear plasmids of Streptomyces and Mycobacterium. This and four previously described methods for actinomycetes telomere cloning and sequencing are discussed.
authors.affiliation: Key laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Fan
authors.forename: Yumei
authors.initials: Y
authors.lastname: Dai
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Cheng
authors.forename: Guangjun
authors.initials: G
authors.lastname: Zhang
authors.forename: Dongshu
authors.initials: D
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Fang
authors.forename: Hang
authors.initials: H
authors.lastname: Wu
authors.forename: Linquan
authors.initials: L
authors.lastname: Bai
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Qin
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D008026:Ligation / Q000379:methods*; D009161:Mycobacterium / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012093:Replicon; D017422:Sequence Analysis, DNA / Q000379:methods; D013302:Streptomyces / Q000235:genetics*; D016615:Telomere