title: Significantly enhanced production of acarbose in fed-batch fermentation with the addition of S-adenosylmethionine.
abstract: Acarbose, a pseudo-oligosaccharide, is widely used clinically in therapies for non-insulin-dependent diabetes. In the present study, S-adenosylmethionine (SAM) was added to selected media in order to investigate its effect on acarbose fermentation by Actinoplanes utahensis ZJB- 08196. Acarbose titer was seen to increase markedly when concentrations of SAM were added over a period of time. The effects of glucose and maltose on the production of acarbose were investigated in both batch and fed-batch fermentation. Optimal acarbose production was observed at relatively low glucose levels and high maltose levels. Based on these results, a further fed-batch experiment was designed so as to enhance the production of acarbose. Fed-batch fermentation was carried out at an initial glucose level of 10 g/l and an initial maltose level of 60 g/l. Then, 12 h post inoculation, 100 micromol/l SAM was added. In addition, 8 g/l of glucose was added every 24 h, and 20 g/l of maltose was added at 96 h. By way of this novel feeding strategy, the maximum titer of acarbose achieved was 6,113 mg/l at 192 h. To our knowledge, the production level of acarbose achieved in this study is the highest ever reported.
authors.affiliation: Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China.
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Sun
authors.forename: Ming-Gang
authors.initials: MG
authors.lastname: Li
authors.forename: Yuan-Shan
authors.initials: YS
authors.lastname: Wang
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
mesh_terms: D020909:Acarbose / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D008848:Micromonosporaceae / Q000254:growth & development / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism*
chemical_list: D003470:Culture Media; D008320:Maltose; D012436:S-Adenosylmethionine; D005947:Glucose; D020909:Acarbose