title: Molecular characterization of bacterial colonization in the preterm and term infant's intestine.
abstract: OBJECTIVE
  To further define patterns of colonising intestinal microflora in newborn infants utilising molecular methods.
  
  
  METHODS
  Ten term and 5 preterm (<32 wk) infants born at the Royal Hospital for Women, Sydney, Australia were enrolled in the present study and followed for 6 mo post partum. Serial stools were collected, DNA was extracted and subjected to PCR-Denaturing Gradient Gel Electrophoresis using a range of primers and sequencing. The effect of gestational length, feeding and delivery method was compared to the pattern of bacterial acquisition.
  
  
  RESULTS
  Intestinal bacterial diversity was lower in preterm compared with term infants. For term infants, bacterial DNA detection rates were not associated with feeding or delivery method, although Enterobacteria and Clostridia were commonly identified. The detection rate of Bifidobacteria was lower in preterm infants than term infants. Potential pathogens were detected in preterm infant samples.
  
  
  CONCLUSIONS
  Preterm infants frequently have aberrant bacterial colonization of the intestine. Further research is now required to determine if this may contribute to adverse health outcomes.
authors.affiliation: School of Biochemistry and Biotechnology, University of New South Wales, Sydney, Australia.
authors.forename: J C
authors.initials: JC
authors.lastname: Hallab
authors.forename: S T
authors.initials: ST
authors.lastname: Leach
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: H M
authors.initials: HM
authors.lastname: Mitchell
authors.forename: J
authors.initials: J
authors.lastname: Oei
authors.forename: K
authors.initials: K
authors.lastname: Lui
authors.forename: A S
authors.initials: AS
authors.lastname: Day
mesh_terms: D001315:Australia; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007234:Infant, Premature; D007422:Intestines / Q000382:microbiology*; D008297:Male