title: A conducting polymer with benzothiadiazole unit: cell based biosensing applications and adhesion properties.
abstract: Poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-benzo[1,2,5]thiadiazole) (PBDT) was electrochemically deposited on graphite electrodes and used as a matrix for microbial biosensing studies. Moreover, protein adsorption property of the surface was investigated using bovine serum albumin (BSA). For the biosensor preparation, after electrochemical deposition of the polymeric matrix, Gluconobacter oxydans cells were immobilized on the modified electrode. Glucose was used as the substrate and biosensor response was followed successfully at -0.7 V vs Ag/AgCl due to the respiratory activity of the cells which is directly proportional with the substrate concentration. Characterizations were carried out in terms of several parameters such as operational and storage stabilities and surface morphologies. Finally, the effect of antimicrobial agent on the cell based response was tested. As a matrix, conducting polymers enable the preparation of sensitive and stable electrochemical microbial biosensors.
authors.affiliation: Faculty of Science, Biochemistry Department, Ege University, 35100 Bornova, Izmir, Turkey. eylulbaskurt@gmail.com
authors.forename: Eylül
authors.initials: E
authors.lastname: Başkurt
authors.forename: Fulya
authors.initials: F
authors.lastname: Ekiz
authors.forename: Dilek Odacı
authors.initials: DO
authors.lastname: Demirkol
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
authors.forename: Levent
authors.initials: L
authors.lastname: Toppare
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D020600:Gluconobacter oxydans / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013830:Thiadiazoles / Q000737:chemistry*
chemical_list: D011108:Polymers; D013830:Thiadiazoles; C015700:benzo-1,2,3-thiadiazole; D012710:Serum Albumin, Bovine