title: Experimental increase of testosterone levels in free-ranging juvenile male African striped mice (Rhabdomys pumilio) induces physiological, morphological, and behavioral changes.
abstract: Testosterone influences sexual differentiation in early development, and activates sexual maturation and sex-related behavior in males during puberty. Testosterone can also influence the expression of male alternative reproductive tactics, by either organizational effects (fixed tactics) or by activational effects (plastic tactics). However, the roles of testosterone in sexual maturation and at the same time the expression of alternative reproductive tactics have been little investigated experimentally, and studies of free-ranging mammals are lacking. We conducted a field experiment in free-ranging juvenile African striped mice (Rhabdomys pumilio), a species with alternative reproductive tactics. Juvenile male striped mice reaching puberty can remain in their family as philopatric group-living males with low testosterone levels, or they can disperse and become solitary living roamers with much higher testosterone levels. We tested whether experimentally increased testosterone levels in non-scrotal juvenile males induces puberty and leads to the expression of the roaming tactic. Testosterone-treated males received the hormone for 15days by silastic implants which were empty in control-treated males. When compared to control-treated males, testosterone-treated males had higher testosterone levels, lower corticosterone levels, and became scrotal with descended testes. Testosterone-treated males also had larger testes, larger epididymides, and showed indication of spermatogenesis. Testosterone-treated males did not become solitary-living roamers, but had larger home ranges than control males. We conclude that testosterone can induce sexual maturation and causes juvenile males to increase their home ranges, maybe to search for dispersal opportunities.
authors.affiliation: Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, Zurich, Switzerland. julien.raynaud@ieu.uzh.ch
authors.forename: Julien
authors.initials: J
authors.lastname: Raynaud
authors.forename: Karin
authors.initials: K
authors.lastname: Müller
authors.forename: Carsten
authors.initials: C
authors.lastname: Schradin
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D003345:Corticosterone / Q000097:blood; D008297:Male; D051379:Mice; D012098:Reproduction / Q000187:drug effects; D012726:Sexual Behavior, Animal / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects; D013739:Testosterone / Q000097:blood / Q000494:pharmacology*
chemical_list: D013739:Testosterone; D003345:Corticosterone