title: Design and characterisation of doxorubicin-releasing chitosan microspheres for anti-cancer chemoembolisation.
abstract: The aims of this study were to design and characterise doxorubicin-loaded chitosan microspheres for anti-cancer chemoembolisation. Doxorubicin-loaded chitosan microspheres were prepared by emulsification and cross-linking methods. Doxorubicin-chitosan solution was initially complexed with tripolyphosphate (TPP) to improve drug loading capabilities. Doxorubicin-loaded chitosan microspheres were highly spherical and had approximately diameters of 130-160 µm in size. Drug loading amount and loading efficiency were in the range 3.7-4.0% and 68.5-85.8%, respectively, and affected by TPP concentration, drug levels and cross-linking time. Doxorubicin release was affected by TPP complexation, cross-linking time and release medium. Especially, lysozyme in release media considerably increased drug release. Synergistic anti-cancer activities of doxorubicin-releasing chitosan microspheres were confirmed to VX2 cells in the rabbit auricle model compared with blank microspheres. Doxorubicin-loaded chitosan microspheres can efficiently be prepared by TPP gelation and cross-linking method and developed as multifunctional anti-cancer embolic material.
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, Korea.
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Park
authors.forename: Su Yeon
authors.initials: SY
authors.lastname: Lee
authors.forename: Ga Hyeon
authors.initials: GH
authors.lastname: Lee
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Chung
authors.forename: Keun Min
authors.initials: KM
authors.lastname: Chang
authors.forename: Byung Kook
authors.initials: BK
authors.lastname: Kwak
authors.forename: Hyo-Jeong
authors.initials: HJ
authors.lastname: Kuh
authors.forename: Jaehwi
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D008297:Male; D008863:Microspheres; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011817:Rabbits
chemical_list: D000903:Antibiotics, Antineoplastic; D003692:Delayed-Action Preparations; D004317:Doxorubicin; D048271:Chitosan