title: Salivary Protein Profiles among HER2/neu-Receptor-Positive and -Negative Breast Cancer Patients: Support for Using Salivary Protein Profiles for Modeling Breast Cancer Progression.
abstract: Purpose. The objective of this study was to compare the salivary protein profiles from individuals diagnosed with breast cancer that were either HER2/neu receptor positive or negative. Methods. Two pooled saliva specimens underwent proteomic analysis. One pooled specimen was from women diagnosed with stage IIa HER2/neu-receptor-positive breast cancer patients (n = 10) and the other was from women diagnosed with stage IIa HER2/neu-receptor-negative cancer patients (n = 10). The pooled samples were trypsinized and the peptides labeled with iTRAQ reagent. Specimens were analyzed using an LC-MS/MS mass spectrometer. Results. The results yielded approximately 71 differentially expressed proteins in the saliva specimens. There were 34 upregulated proteins and 37 downregulated proteins.
authors.affiliation: Department of Diagnostic Sciences, UTHSC, Dental Branch, Behavioral and Biomedical Sciences Building, Rm. 5322, Houston, TX 77054, USA.
authors.forename: Charles F
authors.initials: CF
authors.lastname: Streckfus
authors.forename: Daniel
authors.initials: D
authors.lastname: Arreola
authors.forename: Cynthia
authors.initials: C
authors.lastname: Edwards
authors.forename: Lenora
authors.initials: L
authors.lastname: Bigler