title: Immunological characteristics and response to lipopolysaccharide of mouse lines selectively bred with natural and acquired immunities.
abstract: Genetic improvement of resistance to infectious diseases is a challenging goal in animal breeding. Infection resistance involves multiple immunological characteristics, including natural and acquired immunity. In the present study, we developed an experimental model based on genetic selection, to improve immunological phenotypes. We selectively established three mouse lines based on phagocytic activity, antibody production and the combination of these two phenotypes. We analyzed the immunological characteristics of these lines using a lipopolysaccharide (LPS), which is one of the main components of Gram-negative bacteria. An intense immunological reaction was induced in each of the three mouse lines. Severe loss of body weight and liver damage were observed, and a high level of cytokine messenger RNA was detected in the liver tissue. The mouse line established using a combination of the two selection standards showed unique characteristics relative to the mouse lines selected on the basis of a single phenotype. Our results indicate that genetic selection and breeding is effective, even for immunological phenotypes with a relatively low heritability. Thus, it may be possible to improve resistance to infectious diseases by means of genetic selection.
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Aoba, Sendai, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Narahara
authors.forename: Eri
authors.initials: E
authors.lastname: Sakai
authors.forename: Masafumi
authors.initials: M
authors.lastname: Katayama
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ohtomo
authors.forename: Kanako
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Miki
authors.initials: M
authors.lastname: Takemoto
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
authors.forename: Shyuichi
authors.initials: S
authors.lastname: Ohwada
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Mohri
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Nishimori
authors.forename: Emiko
authors.initials: E
authors.lastname: Isogai
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Fukuda
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D000917:Antibody Formation / Q000502:physiology; D001424:Bacterial Infections; D016207:Cytokines / Q000032:analysis; D004198:Disease Susceptibility; D020022:Genetic Predisposition to Disease / Q000235:genetics; D007113:Immunity, Innate; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D010587:Phagocytosis; D010641:Phenotype; D060888:Real-Time Polymerase Chain Reaction; D012641:Selection, Genetic; D000637:Transaminases / Q000378:metabolism
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D000637:Transaminases