title: [FISH analysis of meiotic segregation results of the spermatozoa from male pericentric inversion carriers].
abstract: OBJECTIVE
  To analyze the meiotic segregation results of the spermatozoa from male pericentric inversion carriers by fluorescence in-situ hybridization (FISH).
  
  
  METHODS
  Using chemical depolymerization and multicolor FISH, we analyzed the meiotic segregation results of the spermatozoa from 4 male pericentric inversion carriers.
  
  
  RESULTS
  Of the 4 males studied, 46,XY,inv(9) (p11q12) was found in 2, 46,XY,inv(9) (p11q13) in 1 and 46,XY,inv(6) (p22q24) in the other; the lengths of the inverted segments represented 16.0, 16.0, 21.0 and 76.0% of the size of the whole chromosome involved; and the frequencies of recombinant sperm were 0.2, 0.4, 0.3 and 43.9%, del(p)/dup(q) accounting for 22.4% and del(q)/dup(p) 21.5%, respectively.
  
  
  CONCLUSIONS
  Males with pericentric inversion may produce spermatozoa with recombinant chromosomes and the rate of recombination varies principally according to the size proportion to the whole chromosome involved. The results of FISH analysis of chromosomal unbalanced spermatozoa can provide accurate personalized information on the genetic risk of fertility.
authors.affiliation: Center of Reproductive Medicine, The First Hospital Affiliated to Wenzhou Medical College, Wenzhou, Zhejiang 325000, China. pana44@163.com
authors.forename: Cheng-Shuang
authors.initials: CS
authors.lastname: Pan
authors.forename: Xiu-Fang
authors.initials: XF
authors.lastname: Qiu
authors.forename: Xi-Xi
authors.initials: XX
authors.lastname: Huang
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Weng
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Huang
mesh_terms: D000328:Adult; D007446:Chromosome Inversion / Q000235:genetics*; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D008540:Meiosis; D013094:Spermatozoa