title: A comparison between different reference genes for expression studies in human hippocampal tissue.
abstract: The reliability of gene expression studies by mRNA quantification is highly dependent upon several experimental procedures, including the choice of reference genes used for data normalization. In order to contribute to gene expression studies in mesial temporal lobe epilepsy (MTLE) we used microarray data, followed by real time quantitative PCR validation of selected housekeeping genes, to determine the most appropriate reference genes to be used in human hippocampal tissue gene expression studies. Our results unequivocally showed a significant impact of the reference gene chosen for normalization on the overall results of expression studies, clearly demonstrating the importance of adequate validation using stable reference genes. In addition, we found that HPRT, NSE, SDHA and SYP are suitable genes to be used as reference for normalization in expression studies of hippocampal tissue obtained from patients with MTLE.
authors.affiliation: Department of Medical Genetics, University of Campinas-UNICAMP, Campinas, SP, Brazil.
authors.forename: Claudia V
authors.initials: CV
authors.lastname: Maurer-Morelli
authors.forename: Jaira F
authors.initials: JF
authors.lastname: de Vasconcellos
authors.forename: Fernanda C
authors.initials: FC
authors.lastname: Reis-Pinto
authors.forename: Cristiane de S
authors.initials: Cde S
authors.lastname: Rocha
authors.forename: Romenia R
authors.initials: RR
authors.lastname: Domingues
authors.forename: Clarissa L
authors.initials: CL
authors.lastname: Yasuda
authors.forename: Helder
authors.initials: H
authors.lastname: Tedeschi
authors.forename: Evandro
authors.initials: E
authors.lastname: De Oliveira
authors.forename: Fernando
authors.initials: F
authors.lastname: Cendes
authors.forename: Iscia
authors.initials: I
authors.lastname: Lopes-Cendes
mesh_terms: D004833:Epilepsy, Temporal Lobe / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards*; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods / Q000592:standards*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D009419:Nerve Tissue Proteins