title: A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus.
abstract: Yeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due to their homothallic character and variable ploidy level. Homothallism is determined by the HO gene in S. cerevisiae and the Sc-HO gene in S. pastorianus. In this work, we describe the construction of an HO disruption vector (pDHO) containing an HO disruption cassette and discuss its use in generating heterothallic yeast strains from homothallic Saccharomyces species.
authors.affiliation: Department of Microbiology, School of Biotechnology, Faculty of Pharmacy, University of Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Blasco
authors.forename: Patricia
authors.initials: P
authors.lastname: Veiga-Crespo
authors.forename: Miquel
authors.initials: M
authors.lastname: Viñas
authors.forename: Tomás G
authors.initials: TG
authors.lastname: Villa
mesh_terms: D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D017353:Gene Deletion; D055786:Gene Knockout Techniques / Q000379:methods*; D005822:Genetic Vectors; D012440:Saccharomyces / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C530845:HO protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific