title: Discovery of potent antimicrobial peptide analogs of Ixosin-B.
abstract: Antimicrobial peptides (AMPs) represent the first defense line against infection when organisms are infected by pathogens. These peptides are generally good targets for the development of antimicrobial agents. Peptide amide analogs of Ixosin-B, an antimicrobial peptide with amino acid sequence of QLKVDLWGTRSGIQPEQHSSGKSDVRRWRSRY, were designed, synthesized and examined for antimicrobial activities against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Within the peptides synthesized, we discovered an 11-mer peptide, KRLRRVWRRWR-amide, which exhibited potent antimicrobial activity while very little hemolytic activity in human erythrocytes was observed even at high dose level (100 μM). With further modifications, this peptide could be developed into a potent antimicrobial agent in the future.
authors.affiliation: Department of Chemistry, Tunghai University, Taichung 407, Taiwan, ROC. fdlung@thu.edu.tw
authors.forename: Feng-Di T
authors.initials: FD
authors.lastname: Lung
authors.forename: Kai-Shiuan
authors.initials: KS
authors.lastname: Wang
authors.forename: Zih-Jie
authors.initials: ZJ
authors.lastname: Liao
authors.forename: Sheng-Kai
authors.initials: SK
authors.lastname: Hsu
authors.forename: Fei-Yi
authors.initials: FY
authors.lastname: Song
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Liou
authors.forename: Yu-Shan
authors.initials: YS
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis* / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006461:Hemolysis; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides