title: Inhibitory effect of melanogenesis by 5-pentyl-2-furaldehyde isolated from Clitocybe sp.
abstract: In the continued search for melanogenesis inhibitors from microbial metabolites, we found that the culture broth of Clitocybe sp. MKACC 53267 inhibited melanogenesis in B16F10 melanoma cells. The active component was purified by solvent extraction, silica gel chromatography, Sephadex LH-20 column chromatography, and finally by preparative HPLC. Its structure was determined as 5- pentyl-2-furaldehyde on the basis of the UV, NMR, and MS spectroscopic analysis. The 5-pentyl-2-furaldehyde potently inhibited melanogenesis in B16F10 cells with an IC50 value of 8.4 microgram/ml, without cytotoxicity.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Choo
authors.forename: In-Ja
authors.initials: IJ
authors.lastname: Ryoo
authors.forename: Bo Yeon
authors.initials: BY
authors.lastname: Kim
authors.forename: Jong Seog
authors.initials: JS
authors.lastname: Ahn
authors.forename: Ick-Dong
authors.initials: ID
authors.lastname: Yoo
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000963:Antimetabolites / Q000302:isolation & purification / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000187:drug effects*; D045744:Cell Line, Tumor; D002845:Chromatography / Q000379:methods; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism*; D020128:Inhibitory Concentration 50; D008543:Melanins / Q000096:biosynthesis*; D051379:Mice; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D000963:Antimetabolites; D008543:Melanins; D005662:Furaldehyde