title: Human infant saliva peptidome is modified with age and diet transition.
abstract: In order to describe developmental changes in human salivary peptidome, whole saliva was obtained from 98 infants followed longitudinally at 3 and 6months of age. Data on teeth eruption and diet at the age of 6months were also recorded. Salivary peptide extracts were characterised by label-free MALDI-MS. Peptides differentially expressed between the two ages, and those significantly affected by teeth eruption or introduction of solid foods were identified by MALDI TOF-TOF and LC ESI MS-MS. Out of 81 peaks retained for statistical analysis, 26 were overexpressed at the age of 6months. Exposure to solid foods had a more pronounced effect on profiles (overexpression of nine peaks) than teeth eruption (overexpression of one peak). Differential peaks corresponded to fragments of acidic and basic PRPs, statherin and histatin. Comparison with existing knowledge on adult saliva peptidome revealed that proteolytic processing of salivary proteins is qualitatively quite comparable in infants and in adults. However, age and diet are modulators of salivary peptidome in human infants.
authors.affiliation: CNRS, UMR6265 Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France. Martine.Morzel@dijon.inra.fr
authors.forename: Martine
authors.initials: M
authors.lastname: Morzel
authors.forename: Aline
authors.initials: A
authors.lastname: Jeannin
authors.forename: Géraldine
authors.initials: G
authors.lastname: Lucchi
authors.forename: Caroline
authors.initials: C
authors.lastname: Truntzer
authors.forename: Delphine
authors.initials: D
authors.lastname: Pecqueur
authors.forename: Sophie
authors.initials: S
authors.lastname: Nicklaus
authors.forename: Christophe
authors.initials: C
authors.lastname: Chambon
authors.forename: Patrick
authors.initials: P
authors.lastname: Ducoroy
mesh_terms: D000328:Adult; D000375:Aging / Q000378:metabolism*; D005247:Feeding Behavior / Q000502:physiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D014078:Tooth Eruption / Q000502:physiology*
chemical_list: D010455:Peptides; D020543:Proteome; D012471:Salivary Proteins and Peptides