title: EUCAST technical note on the EUCAST definitive document EDef 7.2: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST-AFST).
abstract: The European Committee on Antimicrobial Susceptibility Testing-Subcommittee on Antifungal Susceptibility Testing (EUCAST-AFST) has revised the EDef 7.1 document on the method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for fermentative yeasts. Changes are: dimethylsulphoxide is now the recommended solvent for caspofungin, micafungin and fluconazole; the shelf-life of plates containing the echinocandins prepared from stock solutions in dimethylsulphoxide is extended to 6 months at -80°C; testing of amphotericin and Cryptococcus has been incorporated; and minimum inhibitory concentration ranges for quality control strains and anidulafungin are included.
authors.affiliation: Unit of Mycology, Department of Microbiological Surveillance and Research, Statens Serum Institute, Copenhagen, Denmark. maiken@arendrup.dk
authors.forename: Maiken C
authors.initials: MC
authors.lastname: Arendrup
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
authors.forename: Cornelia
authors.initials: C
authors.lastname: Lass-Flörl
authors.forename: William
authors.initials: W
authors.lastname: Hope
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D005060:Europe; D005615:Freezing; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards*; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D015003:Yeasts / Q000187:drug effects*
chemical_list: D000935:Antifungal Agents; D012997:Solvents