title: Enhanced recovery and purification of P(3HB-co-3HHx) from recombinant Cupriavidus necator using alkaline digestion method.
abstract: A simple, efficient and economical method for the recovery of P(3HB-co-3HHx) was developed using various chemicals and parameters. The initial content of P(3HB-co-3HHx) in bacterial cells was 50-60 wt%, whereas the monomer composition of 3HHx used in this experiments was 3-5 mol%. It was found that sodium hydroxide (NaOH) was the most effective chemical for the recovery of biodegradable polymer. High polyhydroxyalkanoate purity and recovery yield both in the range of 80-90 wt% were obtained when 10-30 mg/ml of cells were incubated in NaOH at the concentration of 0.1 M for 60-180 min at 30 °C and polished using 20 % (v/v) of ethanol.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
authors.forename: Siti Nor Syairah
authors.initials: SN
authors.lastname: Anis
authors.forename: M I
authors.initials: MI
authors.lastname: Nurhezreen
authors.forename: K
authors.initials: K
authors.lastname: Sudesh
authors.forename: A A
authors.initials: AA
authors.lastname: Amirul
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D000217:Acyltransferases / Q000235:genetics; D002208:Caproates / Q000737:chemistry* / Q000302:isolation & purification*; D005591:Chemical Fractionation / Q000379:methods*; D020118:Cupriavidus necator / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D004274:DNA, Recombinant / Q000235:genetics*; D055772:Green Chemistry Technology / Q000191:economics / Q000379:methods*; D000073878:Palm Oil; D010938:Plant Oils / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D012972:Sodium Hydroxide / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D000217:Acyltransferases; D002208:Caproates; D004274:DNA, Recombinant; D000073878:Palm Oil; D010938:Plant Oils; D012972:Sodium Hydroxide; D012997:Solvents; D014867:Water; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C077225:poly(3-hydroxyalkanoic acid) synthase