title: Hybrid fluorescent curcumin loaded zein electrospun nanofibrous scaffold for biomedical applications.
abstract: Nanomedicine utilizes engineered nanodevices and nanostructures for monitoring, repair, construction and control of human biological systems at the molecular level. In this study, we investigated the feasibility and potential of zein nanofiber as a delivery vehicle for curcumin in biomedical applications. By optimizing the electrospinning parameters, ultrafine zein fluorescence nanofibers containing curcumin were developed with interconnected fibrous networks. We found that these nanofibers show an increase in fluorescence due to the incorporation of curcumin. The morphology and material properties of the resulting multifunctional nanofiber including the surface area were examined by a field emission-scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and confocal microscopy. The surface area and pore size were characterized by N(2) adsorption-desorption isotherm. SEM and fluorescence images showed that the uniform fibers with smooth surface had an average diameter of about 310 nm. An in vitro degradation study showed significant morphological changes. The in vitro evaluations suggested that the curcumin incorporated zein nanofibers showed sustained release of curcumin and maintained its free radical scavenging ability. It provides an attractive structure for the attachment and growth of fibroblast as cell culture surfaces. The results demonstrate that the curcumin loaded zein nanofiber could be a good candidate for soft tissue engineering scaffolds and has the potential for further applications in drug delivery system.
authors.affiliation: Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama, 350-8585, Japan.
authors.forename: Dhandayuthapani
authors.initials: D
authors.lastname: Brahatheeswaran
authors.forename: Anila
authors.initials: A
authors.lastname: Mathew
authors.forename: Ravindran Girija
authors.initials: RG
authors.lastname: Aswathy
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nagaoka
authors.forename: K
authors.initials: K
authors.lastname: Venugopal
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Toru
authors.initials: T
authors.lastname: Maekawa
authors.forename: D
authors.initials: D
authors.lastname: Sakthikumar
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques; D003474:Curcumin / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D005347:Fibroblasts / Q000166:cytology; D016166:Free Radical Scavengers; D005609:Free Radicals; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D057139:Nanofibers / Q000737:chemistry*; D050997:Nanomedicine / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013818:Thermogravimetry / Q000379:methods; D023822:Tissue Engineering; D015028:Zein / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D016166:Free Radical Scavengers; D005609:Free Radicals; D015028:Zein; D003474:Curcumin