title: Lipoproteins of Actinomyces viscosus induce inflammatory responses through TLR2 in human gingival epithelial cells and macrophages.
abstract: Actinomyces viscosus has been suggested to be associated with periodontal disease. However, the pathogenicity of this bacterium is not known. In this study, we examined inflammation-inducing activity by A. viscosus. Whole cells and a lipophilic fraction of A. viscosus ATCC19246 induced production of interleukin-8 and tumor necrosis factor alpha from both human oral epithelial cells and human monocytoid cells. This cytokine production was blocked by lipoprotein lipase treatment of the lipophilic fraction. In addition, anti-Toll-like receptor 2 antibody blocked the cytokine production. These results suggest that lipoprotein of A. viscosus triggers inflammatory responses in periodontitis by activation of Toll-like receptor 2.
authors.affiliation: Department of Oral Microbiology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Shimada
authors.forename: Hideo
authors.initials: H
authors.lastname: Kataoka
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Miyazawa
authors.forename: Matsuo
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Igarashi
mesh_terms: D016924:Actinomyces viscosus / Q000737:chemistry / Q000276:immunology*; D000196:Actinomycosis / Q000276:immunology / Q000382:microbiology; D000704:Analysis of Variance; D001426:Bacterial Proteins / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology; D005881:Gingiva / Q000166:cytology / Q000276:immunology*; D005882:Gingival Diseases / Q000276:immunology / Q000382:microbiology; D057809:HEK293 Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D007249:Inflammation / Q000276:immunology; D008074:Lipoproteins / Q000276:immunology*; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D008074:Lipoproteins; D051195:Toll-Like Receptor 2