title: Contact-dependent interference with invariant NKT cell activation by herpes simplex virus-infected cells.
abstract: Invariant CD1d-restricted NKT (iNKT) cells play important roles in generating protective immune responses against infections. In this study, we have investigated the role of human iNKT cells in HSV-1 infection and their interaction with epidermal keratinocytes. These cells express CD1d and are the primary target of the virus. Keratinocytes loaded with α-galactosyl ceramide (α-GalCer) could stimulate IFN-γ production and CD25 upregulation by iNKT cells. However, both α-GalCer-dependent and cytokine-dependent activation of iNKT cells was impaired after coculture with HSV-1-infected cells. Notably, CD1d downregulation was not observed on infected keratinocytes, which were also found to inhibit TCR-independent iNKT cell activation. Further examination of the cytokine profile of iNKT-keratinocyte cocultures showed inhibition of IFN-γ, IL-5, IL-10, IL-13, and IL-17 secretion but upregulation of IL-4 and TNF-α after the infection. Moreover, cell-to-cell contact between infected keratinocytes and iNKT cells was required for the inhibition of activation, as the cell-free supernatants containing virus did not affect activation. Productive infection of iNKT cells was however not required for the inhibitory effect. After coculture with infected cells, iNKT cells were no longer responsive to further stimulation with α-GalCer-loaded CD1d-expressing cells. We found that exposure to HSV-1-infected cells resulted in impaired TCR signaling downstream of ZAP70. Additionally, infected cells upregulated the expression of the negative T cell regulator, galectin-9; however, blocking experiments indicated that the impairment of iNKT cell responses was independent of galectin-9. Thus, interference with activation of human iNKT cells by HSV-1 may represent a novel immunoevasive strategy used by the virus to avoid immune clearance.
authors.affiliation: Department of Medicine, Center for Infectious Medicine, Karolinska Institute, Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden. lidija.bosnjak@ki.se
authors.forename: Lidija
authors.initials: L
authors.lastname: Bosnjak
authors.forename: Peter
authors.initials: P
authors.lastname: Sahlström
authors.forename: Dominic
authors.initials: D
authors.lastname: Paquin-Proulx
authors.forename: Edwin
authors.initials: E
authors.lastname: Leeansyah
authors.forename: Markus
authors.initials: M
authors.lastname: Moll
authors.forename: Johan K
authors.initials: JK
authors.lastname: Sandberg
mesh_terms: D002450:Cell Communication / Q000276:immunology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis; D006561:Herpes Simplex / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000276:immunology / Q000378:metabolism / Q000821:virology*; D008213:Lymphocyte Activation / Q000276:immunology*; D055611:Natural Killer T-Cells / Q000276:immunology* / Q000378:metabolism; D018139:Simplexvirus / Q000276:immunology
chemical_list: D016207:Cytokines