title: Role of PKCα-p(38)MAPK-G(i)α axis in NADPH oxidase derived O(2)(·-)-mediated activation of cPLA(2) under U46619 stimulation in pulmonary artery smooth muscle cells.
abstract: We have recently reported that treatment of bovine pulmonary artery smooth muscle cells with the thromboxane A(2) mimetic, U46619 stimulated NADPH oxidase derived O(2)(·-) level, which subsequently caused marked increase in [Ca(2+)](i)[17]. Herein, we demonstrated that O(2)(·-)-mediated increase in [Ca(2+)](i) stimulates an aprotinin sensitive proteinase activity, which proteolytically activates PKC-α under U46619 treatment to the cells. The activated PKC-α then phosphorylates p(38)MAPK and that subsequently caused G(i)α phosphorylation leading to stimulation of cPLA(2) activity in the cell membrane.
authors.affiliation: Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, West Bengal, India. sajal_chakraborti@yahoo.com
authors.forename: Sajal
authors.initials: S
authors.lastname: Chakraborti
authors.forename: Soumitra
authors.initials: S
authors.lastname: Roy
authors.forename: Amritlal
authors.initials: A
authors.lastname: Mandal
authors.forename: Kuntal
authors.initials: K
authors.lastname: Dey
authors.forename: Animesh
authors.initials: A
authors.lastname: Chowdhury
authors.forename: Soni
authors.initials: S
authors.lastname: Shaikh
authors.forename: Tapati
authors.initials: T
authors.lastname: Chakraborti
mesh_terms: D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / Q000494:pharmacology*; D000818:Animals; D040761:Biomimetic Materials / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism*; D054510:Phospholipases A2, Cytosolic / Q000378:metabolism*; D051571:Protein Kinase C-alpha / Q000235:genetics / Q000378:metabolism; D011651:Pulmonary Artery / Q000166:cytology*; D013481:Superoxides / Q000378:metabolism*; D013928:Thromboxane A2 / Q000378:metabolism; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D014662:Vasoconstrictor Agents; D013481:Superoxides; D013928:Thromboxane A2; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D019255:NADPH Oxidases; D051571:Protein Kinase C-alpha; D048051:p38 Mitogen-Activated Protein Kinases; D054510:Phospholipases A2, Cytosolic; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D002118:Calcium