title: Synergism of Staphylococcus aureus colonization and allergic reaction in the nasal cavity in mice.
abstract: BACKGROUND
  The aim of this study is to investigate the reciprocal effect of Staphylococcus aureus colonization and allergic rhinitis in an allergy model of mice.
  
  
  METHODS
  BALB/c mice with intraperitoneal ovalbumin (OVA) sensitization and/or intranasal S. aureus inoculation were prepared. The following 4 groups were designed: an OVA-sensitized S. aureus-inoculated (AR-SA) group, an OVA-sensitized uninoculated (AR) group, a nonsensitized S. aureus-inoculated (SA) group, and a nonsensitized uninoculated (control) group. After intranasal OVA challenge, nasal lavage fluid, peripheral blood, and nasal mucosa were collected. Polymorphonuclear cells in the nasal lavage fluid were counted, serum OVA-specific IgE and IgG1 were measured by enzyme immunoassays, and IL-4, IL-5, and IFN-γ mRNAs in the nasal mucosa were assessed by quantitative real-time reverse transcription-PCR. The number of S. aureus in the nasal mucosa and lavage fluid was counted.
  
  
  RESULTS
  Both eosinophil and neutrophil counts were larger in the AR-SA group than in the other groups. Both IgE and IgG1 levels were higher in the AR and AR-SA groups than in the SA and control groups, and the IgG1 level was higher in the AR-SA group than in the AR group. The expression of IL-4 mRNA was higher in the AR-SA group than in the other groups, and the expression of IL-5 mRNA was higher in the AR-SA group than in the SA group. The AR-SA group showed higher counts of S. aureus in the nasal mucosa than the SA group.
  
  
  CONCLUSIONS
  These results indicate the mutually potentiating effect of S. aureus colonization and allergic rhinitis.
authors.affiliation: Department of Otorhinolaryngology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Nobusuke
authors.initials: N
authors.lastname: Hohchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Hashida
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Ohkubo
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Wakasugi
authors.forename: Takanori
authors.initials: T
authors.lastname: Mori
authors.forename: Khac-Hung
authors.initials: KH
authors.lastname: Nguyen
authors.forename: Etsushi
authors.initials: E
authors.lastname: Kuroda
authors.forename: Takako
authors.initials: T
authors.lastname: Ikeno
authors.forename: Hatsumi
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D058491:Bacterial Load; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D004804:Eosinophils / Q000276:immunology; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009296:Nasal Cavity / Q000276:immunology* / Q000382:microbiology; D017817:Nasal Lavage Fluid / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D009297:Nasal Mucosa / Q000276:immunology* / Q000382:microbiology; D009504:Neutrophils / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D013203:Staphylococcal Infections / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification
chemical_list: D000485:Allergens; D016207:Cytokines; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D010047:Ovalbumin