title: Survivin regulates Plk1 localization to kinetochore in mouse oocyte meiosis.
abstract: Survivin is a member of inhibitors of apoptosis proteins (IAPs), and also belongs to be a member of the chromosomal passenger complex (CPC) which has multiple functions including inhibition of apoptosis and regulation of cell division and SAC activity. Plk1 (polo-like kinase 1) associates with the spindle poles and also distributes to the kinetochores and is shown to involve in spindle organization, APC/C activation and cytokinesis in many models. Our recent work has shown that Survivin is a critical regulator of chromosome segregation and spindle assembly checkpoint (SAC) in meiosis. In the present study, we found that Plk1 co-localized with Survivin at metaphase I (MI) and telophase I (TI) stage after GVBD. Plk1 dispersed into the oocyte cytoplasm or accumulated near the chromosomes after the depletion of Survivin by morpholino (MO) injection. Our results showed that the localization of Plk1 to kinetochores required the involvement of Survivin.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. sunsc@njau.edu.cn
authors.forename: Shao-Chen
authors.initials: SC
authors.lastname: Sun
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Liu
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D055785:Gene Knockdown Techniques; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D018386:Kinetochores / Q000201:enzymology*; D008540:Meiosis; D008677:Metaphase; D051379:Mice; D060172:Morpholinos / Q000494:pharmacology; D009865:Oocytes / Q000166:cytology* / Q000201:enzymology; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D008941:Spindle Apparatus / Q000201:enzymology; D000077022:Survivin; D013692:Telophase; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D051034:Inhibitor of Apoptosis Proteins; D060172:Morpholinos; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D000077022:Survivin; D000097869:Polo-Like Kinase 1; C117749:Birc5 protein, mouse