title: The responses of the hepatic and splanchnic vascular beds to vasopressin in rats.
abstract: Vasopressin, a vasoactive peptide, causes vasoconstriction via V1a vasopressin receptors. Unlike other vasoconstrictor agents, vasopressin also has vasodilatory properties. The purpose of this study was to determine the effect of vasopressin on hepatic and splanchnic circulation in Sprague- Dawley rats. The experiments were conducted in not only isolated blood- and constant flowperfused livers but also anesthetized spontaneously breathing rats. In anesthetized rats, portal venous pressure (Ppv), systemic arterial pressure (Psa), central venous pressure, and hepatic blood flow (HBF) of combined portal venous and hepatic arterial blood flow were continuously measured, and splanchnic vascular bed resistance (Rspl) defined by (Psa - Ppv) / HBF was determined. In perfused livers, vasopressin at 0.1-1,000 nM caused weak venoconstriction as evidenced by small increase in Ppv. In anesthetized rats, when vasopressin was injected into the portal vein as a bolus consecutively at 0.01-100 nmol/kg, Psa increased dose-dependently with the peak increment of 60 ± 18 mmHg at 100 nmol/kg. Ppv and HBF decreased, with resultant increase in Rspl, indicating splanchnic vasoconstriction. In conclusion, hepatic venoconstrictor action of vasopressin was weak in rats. Vasopressin causes splanchnic vasoconstriction, resulting in a decrease in HBF and Ppv in anesthetized rats.
authors.affiliation: Department of Physiology II, Kanazawa Medical University, Uchinada Ishikawa 920-0293, Japan.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Toshishige
authors.initials: T
authors.lastname: Shibamoto
authors.forename: Yuhichi
authors.initials: Y
authors.lastname: Kuda
authors.forename: Shohei
authors.initials: S
authors.lastname: Shinomiya
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Kurata
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure / Q000187:drug effects; D066298:In Vitro Techniques; D008099:Liver / Q000098:blood supply*; D008297:Male; D017082:Portal Pressure / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013152:Splanchnic Circulation / Q000187:drug effects*; D014662:Vasoconstrictor Agents / Q000494:pharmacology*; D014667:Vasopressins / Q000494:pharmacology*
chemical_list: D014662:Vasoconstrictor Agents; D014667:Vasopressins