title: A novel bFGF antagonist peptide inhibits breast cancer cell growth.
abstract: Breast cancer is the most common type of cancer in women worldwide. Elevated expression of the basic fibroblast growth factor (bFGF) has been found in patients suffering from breast cancer. We previously obtained a high-afﬁnity bFGF-binding peptide (named P7) from a phage-display random heptapeptide library. In this study, we show that P7 peptides significantly inhibits the proliferation of the bFGF-stimulated MDA-MB-231 breast cancer cell line. Additional experiments revealed that the mechanisms of the P7 peptide inhibition of the cell proliferation of breast cancer cells stimulated with bFGF in vitro involved cell cycle arrest at the G0/G1 phase, blockade of the activation of Erk and P38 cascades and the upregulation of the expression of the growth inhibitor, proliferation-associated protein 2G4. These results suggest that the bFGF-binding peptide may have therapeutic potential in breast cancer therapy.
authors.affiliation: Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou, Guangdong 510632, PR China.
authors.forename: Quchou
authors.initials: Q
authors.lastname: Li
authors.forename: Susu
authors.initials: S
authors.lastname: Gao
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Yu
authors.forename: Wenhui
authors.initials: W
authors.lastname: Wang
authors.forename: Xilei
authors.initials: X
authors.lastname: Chen
authors.forename: Ruixue
authors.initials: R
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D016601:RNA-Binding Proteins / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D006131:Growth Inhibitors; D009842:Oligopeptides; C101633:PA2G4 protein, human; D010455:Peptides; D016601:RNA-Binding Proteins; C574793:prolyl-leucyl-leucyl-glutaminyl-alanyl-threonyl-leucine; D016222:Fibroblast Growth Factor 2; D020928:Mitogen-Activated Protein Kinases