title: [Establishment of detection system of CNVs HMGR, SQS1, beta-AS synthase gene of Glycyrrhiza uralensis].
abstract: OBJECTIVE
  To establish a stabilized and reliable detection system of CNVs of HMGR, SQS1, beta-AS gene of Glycyrrhiza uralensis.
  
  
  METHODS
  Real time PCR was used to detect the CNVs of HMGR, SQS1, beta-AS gene of G. uralensis.
  
  
  RESULTS
  In the quantitative detection experiments of HMGR, SQS1, beta-AS gene of G. uralensis, the change of value of C(t) was 25.82-25.88, 29.01-29. 08, 15.52-15.56, 19.06-19.08 respectively, the alue of SD was 0.033, 0.032, 0.024, 0.011 respectively, and the value of CV was 0.12%, 0.22%, 0.16%, 0.06% respectively.
  
  
  CONCLUSIONS
  The repeatability of detection system of Real time PCR was stabilized and reliable, and the method could be used to detect the CNVs of HMGR, SQS1, beta-AS gene of G. uralensis.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. liuyliwd@vip.sina.com.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Dongji
authors.initials: D
authors.lastname: Liu
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Liu
mesh_terms: D056915:DNA Copy Number Variations; D018744:DNA, Plant; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000235:genetics*; D029882:Glycyrrhiza uralensis / Q000201:enzymology / Q000235:genetics*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D058889:Molecular Typing / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D006903:Hydroxymethylglutaryl CoA Reductases; D013186:Farnesyl-Diphosphate Farnesyltransferase