title: Intermediate frequency magnetic field at 23 kHz does not modify gene expression in human fetus-derived astroglia cells.
abstract: The increased use of induction heating (IH) cooktops in Japan and Europe has raised public concern on potential health effects of the magnetic fields generated by IH cooktops. In this study, we evaluated the effects of intermediate frequency (IF) magnetic fields generated by IH cooktops on gene expression profiles. Human fetus-derived astroglia cells were exposed to magnetic fields at 23 kHz and 100 µT(rms) for 2, 4, and 6 h and gene expression profiles in cells were assessed using cDNA microarray. There were no detectable effects of the IF magnetic fields at 23 kHz on the gene expression profile, whereas the heat treatment at 43 °C for 2 h, as a positive control, affected gene expression including inducing heat shock proteins. Principal component analysis and hierarchical analysis showed that the gene profiles of IF-exposed groups were similar to the sham-exposed group and were different than the heat treatment group. These results demonstrated that exposure of human fetus-derived astroglia cells to an IF magnetic field at 23 kHz and 100 µT(rms) for up to 6 h did not induce detectable changes in gene expression profile.
authors.affiliation: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Sakurai
authors.forename: Eijiro
authors.initials: E
authors.lastname: Narita
authors.forename: Naoki
authors.initials: N
authors.lastname: Shinohara
authors.forename: Junji
authors.initials: J
authors.lastname: Miyakoshi
mesh_terms: D001253:Astrocytes / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D002453:Cell Cycle / Q000528:radiation effects; D002460:Cell Line; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005333:Fetus / Q000166:cytology*; D006801:Humans; D025341:Principal Component Analysis; D013696:Temperature; D013997:Time Factors; D059467:Transcriptome / Q000528:radiation effects*