title: Metabonomic investigation on the protective effects of rosiglitazone and caloric restriction for renal senescence in a rat model.
abstract: OBJECTIVE
  A liquid chromatography coupled with mass spectrometry based metabonomics approach was applied to investigate the protective effects of rosiglitazone (RGTZ) and caloric restriction (CR) for renal senescence in a rat model.
  
  
  METHODS
  Kidney tissues and serum samples were collected from four groups of rats, including 12- month and 24-month ad libitum fed rats, RGTZ and CR treated 24-month rats. Multivariate data analysis was performed on the mass data of metabonomic profiles to detect the differences among the groups.
  
  
  RESULTS
  By metabolite profiling and partial least squares discriminate analysis, 23 renal senescence-related endogenous metabolites were discovered, including phospholipids, carnitine, acetylcarnitine, and creatinine, most of which were related to the oxidative stress and lipid metabolism.
  
  
  CONCLUSIONS
  Renal senescence is characterized by oxidative stress and changes in lipid metabolism, and RGTZ administration and CR treatment may have similar protective effects for renal senescence via restraining oxidative stress and lipid metabolism.
authors.affiliation: Kidney Institute of PLA, Division of Nephrology, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China.
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shikai
authors.initials: S
authors.lastname: Yan
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Weixing
authors.initials: W
authors.lastname: Dai
authors.forename: Senyan
authors.initials: S
authors.lastname: Liu
authors.forename: Huizi
authors.initials: H
authors.lastname: Jin
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.forename: Changlin
authors.initials: C
authors.lastname: Mei
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D031204:Caloric Restriction; D016922:Cellular Senescence; D004195:Disease Models, Animal; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D007668:Kidney / Q000378:metabolism / Q000503:physiopathology*; D016018:Least-Squares Analysis; D050356:Lipid Metabolism; D008297:Male; D055432:Metabolomics / Q000379:methods*; D015999:Multivariate Analysis; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000627:therapeutic use*; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D007004:Hypoglycemic Agents; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D001616:beta-Galactosidase