title: Resistance to voriconazole due to a G448S substitution in Aspergillus fumigatus in a patient with cerebral aspergillosis.
abstract: A voriconazole-resistant isolate of Aspergillus fumigatus was recovered from an immunocompetent patient receiving long-term antifungal therapy for cerebral aspergillosis. A G448S amino acid substitution in the azole target (Cyp51A) was identified as the cause of the resistance phenotype. This article describes the first isolation of a voriconazole-resistant A. fumigatus isolate from an immunocompetent patient in Spain.
authors.affiliation: Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Universidad Complutense, Madrid, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Pelaez
authors.forename: Paloma
authors.initials: P
authors.lastname: Gijón
authors.forename: Eleonora
authors.initials: E
authors.lastname: Bunsow
authors.forename: Emilio
authors.initials: E
authors.lastname: Bouza
authors.forename: Waldo
authors.initials: W
authors.lastname: Sánchez-Yebra
authors.forename: Maricela
authors.initials: M
authors.lastname: Valerio
authors.forename: Beatriz
authors.initials: B
authors.lastname: Gama
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
authors.forename: Emilia
authors.initials: E
authors.lastname: Mellado
mesh_terms: D019943:Amino Acid Substitution; D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D025141:Drug Resistance, Fungal; D005260:Female; D005656:Fungal Proteins / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D020125:Mutation, Missense; D020953:Neuroaspergillosis / Q000382:microbiology*; D011743:Pyrimidines / Q000494:pharmacology*; D013030:Spain; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D011743:Pyrimidines; D014230:Triazoles; D003577:Cytochrome P-450 Enzyme System; C465526:cytochrome P-450 CYP51A, Aspergillus; D065819:Voriconazole