title: Altered Prion protein expression pattern in CSF as a biomarker for Creutzfeldt-Jakob disease.
abstract: Creutzfeldt-Jakob disease (CJD) is the most frequent human Prion-related disorder (PrD). The detection of 14-3-3 protein in the cerebrospinal fluid (CSF) is used as a molecular diagnostic criterion for patients clinically compatible with CJD. However, there is a pressing need for the identification of new reliable disease biomarkers. The pathological mechanisms leading to accumulation of 14-3-3 protein in CSF are not fully understood, however neuronal loss followed by cell lysis is assumed to cause the increase in 14-3-3 levels, which also occurs in conditions such as brain ischemia. Here we investigated the relation between the levels of 14-3-3 protein, Lactate dehydrogenase (LDH) activity and expression of the prion protein (PrP) in CSF of sporadic and familial CJD cases. Unexpectedly, we found normal levels of LDH activity in CJD cases with moderate levels of 14-3-3 protein. Increased LDH activity was only observed in a percentage of the CSF samples that also exhibited high 14-3-3 levels. Analysis of the PrP expression pattern in CSF revealed a reduction in PrP levels in all CJD cases, as well as marked changes in its glycosylation pattern. PrP present in CSF of CJD cases was sensitive to proteases. The alterations in PrP expression observed in CJD cases were not detected in other pathologies affecting the nervous system, including cases of dementia and tropical spastic paraparesis/HTLV-1 associated myelopathy (HAM/TSP). Time course analysis in several CJD patients revealed that 14-3-3 levels in CSF are dynamic and show a high degree of variability during the end stage of the disease. Post-mortem analysis of brain tissue also indicated that 14-3-3 protein is upregulated in neuronal cells, suggesting that its expression is modulated during the course of the disease. These results suggest that a combined analysis of 14-3-3 and PrP expression pattern in CSF is a reliable biomarker to confirm the clinical diagnosis of CJD patients and follow disease progression.
authors.affiliation: Neuroscience Biomedical Institute, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Torres
authors.forename: Luis
authors.initials: L
authors.lastname: Cartier
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Matamala
authors.forename: Nancy
authors.initials: N
authors.lastname: Hernández
authors.forename: Ute
authors.initials: U
authors.lastname: Woehlbier
authors.forename: Claudio
authors.initials: C
authors.lastname: Hetz
mesh_terms: D048948:14-3-3 Proteins / Q000134:cerebrospinal fluid; D015415:Biomarkers / Q000134:cerebrospinal fluid / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000201:enzymology / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D005786:Gene Expression Regulation; D006031:Glycosylation; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged; D011328:Prions / Q000134:cerebrospinal fluid* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D048948:14-3-3 Proteins; D015415:Biomarkers; D011328:Prions; D007770:L-Lactate Dehydrogenase