title: Fast self-assembly kinetics of quantum dots and a dendrimeric peptide ligand.
abstract: Engineered peptide ligands with exceptionally high affinity for metal can self-assemble with nanoparticles in biological fluids. A high-affinity dendrimeric peptide ligand for CdSe-ZnS quantum dots (QDs) exhibited very fast association kinetics with QDs and reached equilibrium within 2 s. Here, we have combined a droplet-based microfluidic device with fluorescence detection based on Förster resonance energy transfer (FRET) to provide subsecond resolution in dissecting this fast self-assembly kinetics in solution. This work represents the first application of microfluidic devices to ligand-particle assembly for the measurement of fast assembly kinetics in solution.
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, China.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Wang
authors.forename: Pengju
authors.initials: P
authors.lastname: Jiang
authors.forename: Zuoyan
authors.initials: Z
authors.lastname: Han
authors.forename: Lin
authors.initials: L
authors.lastname: Qiu
authors.forename: Cheli
authors.initials: C
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.forename: Jiang
authors.initials: J
authors.lastname: Xia
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry; D019281:Dimerization; D007700:Kinetics; D008024:Ligands; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D008024:Ligands; D010455:Peptides; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C058667:cadmium selenide; C031238:zinc sulfide