title: Activity-dependent neuroprotector homeobox protein: A candidate protein identified in serum as diagnostic biomarker for Alzheimer's disease.
abstract: Alzheimer's disease (AD) is the most common cause of dementia of late life. To enhance our understanding of AD proteome, the serum proteins were analyzed using two-dimensional gel electrophoresis (2DE) combined with nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC-ESI-MS/MS) followed by peptide fragmentation patterning. In this study, six protein spots with differential expression were identified. Five up-regulated proteins were identified as actin, apolipoprotein A-IV (Apo A-IV), inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), alpha-1-antitrypsin (AAT), and antithrombin-III (AT-III); one protein, activity-dependent neuroprotector homeobox protein (ADNP) was down-regulated in AD patients. These proteins with differential expression in the serum may serve as potential indicators of AD. Our results suggested that ADNP may play an important role in slowing the progression of clinical symptoms of AD.
authors.affiliation: Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan.
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Yang
authors.forename: Yuan-Han
authors.initials: YH
authors.lastname: Yang
authors.forename: Chi-Yu
authors.initials: CY
authors.lastname: Lu
authors.forename: Shiang-Bin
authors.initials: SB
authors.lastname: Jong
authors.forename: Li-Jhen
authors.initials: LJ
authors.lastname: Chen
authors.forename: Yu-Fen
authors.initials: YF
authors.lastname: Lin
authors.forename: Shyh-Jong
authors.initials: SJ
authors.lastname: Wu
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Chu
authors.forename: Tze-Wen
authors.initials: TW
authors.lastname: Chung
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Tyan
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood* / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D018398:Homeodomain Proteins / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: C492178:ADNP protein, human; D015415:Biomarkers; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins