title: SRM targeted proteomics in search for biomarkers of HCV-induced progression of fibrosis to cirrhosis in HALT-C patients.
abstract: The current gold standard for diagnosis of hepatic fibrosis and cirrhosis is the traditional invasive liver biopsy. It is desirable to assess hepatic fibrosis with noninvasive means. Targeted proteomic techniques allow an unbiased assessment of proteins and might be useful to identify proteins related to hepatic fibrosis. We utilized selected reaction monitoring (SRM) targeted proteomics combined with an organ-specific blood protein strategy to identify and quantify 38 liver-specific proteins. A combination of protein C and retinol-binding protein 4 in serum gave promising preliminary results as candidate biomarkers to distinguish patients at different stages of hepatic fibrosis due to chronic infection with hepatitis C virus (HCV). Also, alpha-1-B glycoprotein, complement factor H and insulin-like growth factor binding protein acid labile subunit performed well in distinguishing patients from healthy controls.
authors.affiliation: Institute for Systems Biology, Seattle, Washington 98109, USA.
authors.forename: Shizhen
authors.initials: S
authors.lastname: Qin
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Anna S
authors.initials: AS
authors.lastname: Lok
authors.forename: Alex
authors.initials: A
authors.lastname: Tsodikov
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Yan
authors.forename: Li
authors.initials: L
authors.lastname: Gray
authors.forename: Min
authors.initials: M
authors.lastname: Yuan
authors.forename: Robert L
authors.initials: RL
authors.lastname: Moritz
authors.forename: David
authors.initials: D
authors.lastname: Galas
authors.forename: Gilbert S
authors.initials: GS
authors.lastname: Omenn
authors.forename: Leroy
authors.initials: L
authors.lastname: Hood
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000032:analysis* / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D017242:Complement Factor H / Q000032:analysis; D005260:Female; D005355:Fibrosis / Q000097:blood / Q000150:complications / Q000175:diagnosis; D006023:Glycoproteins / Q000032:analysis; D019698:Hepatitis C, Chronic / Q000097:blood / Q000150:complications / Q000175:diagnosis*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D018969:Insulin-Like Growth Factor Binding Proteins / Q000032:analysis / Q000097:blood; D008099:Liver / Q000737:chemistry / Q000473:pathology; D008103:Liver Cirrhosis / Q000097:blood / Q000150:complications / Q000175:diagnosis*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011486:Protein C / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D054839:Retinol-Binding Proteins, Plasma / Q000032:analysis
chemical_list: C048566:A1BG protein, human; D015415:Biomarkers; D001798:Blood Proteins; D006023:Glycoproteins; D007136:Immunoglobulins; D018969:Insulin-Like Growth Factor Binding Proteins; D011486:Protein C; C101326:RBP4 protein, human; D054839:Retinol-Binding Proteins, Plasma; D017242:Complement Factor H