title: Biogenesis of Y RNA-derived small RNAs is independent of the microRNA pathway.
abstract: Y RNAs are approximately 100 nucleotide long conserved cytoplasmic non-coding RNAs, which produce smaller RNA fragments during apoptosis. Here we show that these smaller RNA molecules are also produced in non-stressed cells and in a range of human cancerous and non-cancerous cell types. Recent reports have speculated that the cleavage products of Y RNAs enter the microRNA pathway. We tested this hypothesis and found that Y5 and Y3 RNA fragments are Dicer independent, they are in different complexes than microRNAs and that they are not co-immunoprecipitated with Ago2. Therefore we conclude that Y RNA fragments do not enter the microRNA pathway.
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich, UK.
authors.forename: Francisco Esteban
authors.initials: FE
authors.lastname: Nicolas
authors.forename: Adam E
authors.initials: AE
authors.lastname: Hall
authors.forename: Tibor
authors.initials: T
authors.lastname: Csorba
authors.forename: Carly
authors.initials: C
authors.lastname: Turnbull
authors.forename: Tamas
authors.initials: T
authors.lastname: Dalmay
mesh_terms: D060565:Argonaute Proteins / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000502:physiology; D045325:HCT116 Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D022661:RNA, Untranslated / Q000378:metabolism*; D043244:Ribonuclease III / Q000502:physiology; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; D008565:Membrane Proteins; D035683:MicroRNAs; D022661:RNA, Untranslated; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases