title: Effect of AndoSan™ on expression of adhesion molecules and production of reactive oxygen species in human monocytes and granulocytes in vivo.
abstract: BACKGROUND
  Oral intake (60 ml daily) over 12 days in eight healthy volunteers of an immunostimulatory extract based on the medicinal mushroom Agaricus blazei Murill (AbM (AndoSan™)), reduced the monocyte and granulocyte release of mainly proinflammatory cytokines in vivo, suggesting an anti-inflammatory effect. In this foremost in vivo study, the aim was to examine the effect of such AndoSan™ consumption on the expression of adhesion molecules CD11b, CD11c and CD62L and production of reactive oxygen species (ROS) in leukocytes.
  
  
  RESULTS
  As shown by flow cytometry, there was a significant increase of CD62L expression on monocytes and granulocytes from before (day 0) compared with 12 days after daily AndoSan™ consumption. However, only minor alterations and no clear trend in the expression of CD11b and CD11c were detected. Intracellular ROS (mainly superoxide ion) were significantly reduced in these cells from days 0 to 12.
  
  
  CONCLUSIONS
  These results support that oral intake of AndoSan™ exhibits an anti-inflammatory effect in humans in vivo.
authors.affiliation: Department of Gastroenterological Surgery, Oslo University Hospital, Oslo, Norway. egil.johnson@ulleval.no
authors.forename: Egil
authors.initials: E
authors.lastname: Johnson
authors.forename: Dag Tidemann
authors.initials: DT
authors.lastname: Førland
authors.forename: Geir
authors.initials: G
authors.lastname: Hetland
authors.forename: Lisbeth
authors.initials: L
authors.lastname: Sætre
authors.forename: Ole Kristoffer
authors.initials: OK
authors.lastname: Olstad
authors.forename: Torstein
authors.initials: T
authors.lastname: Lyberg
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000364:Agaricus / Q000737:chemistry*; D000704:Analysis of Variance; D039481:CD11b Antigen / Q000378:metabolism; D039521:CD11c Antigen / Q000378:metabolism; D045424:Complex Mixtures; D005260:Female; D005434:Flow Cytometry; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D019041:L-Selectin / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014020:Tissue Extracts / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: C579023:AndoSan; D039481:CD11b Antigen; D039521:CD11c Antigen; D045424:Complex Mixtures; D017382:Reactive Oxygen Species; D014020:Tissue Extracts; D019041:L-Selectin