title: [Changes in the expression of T lymphocyte subsets in ageing mice induced by D-galactose].
abstract: OBJECTIVE
  To explore the changes and significance of spleen T cell subsets in ageing mice induced by D-galactose.
  
  
  METHODS
  Ageing mice model was successfully established by 100 g/L D-galactose. The content of IFN-γ and IL-4 in serum was measured by ELISA. T cell subsets were detected by Immunofluorescence technique and flow cytometry.
  
  
  RESULTS
  The content of IFN-γ and IL-4 in the serum was decreased of model group (P<0.01).   The naive T cell related  molecule, CD45RA was decreased(P<0.05).  T cell activation-related molecule, CD25 was decreased(P<0.05).   The Foxp3 in CD4(+);CD25(+); T cell was increased(P<0.01).
  
  
  CONCLUSIONS
  In spleen of the ageing mice, the percentage of naive and active T cell are decreased, but The percentage of CD4(+); Tr subset is increased.
authors.affiliation: Department of Immunology, Soochow University, Suzhou, China. zhangyj0815@126.com
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Hua-ting
authors.initials: HT
authors.lastname: Zhu
authors.forename: Sai-nan
authors.initials: SN
authors.lastname: Huang
authors.forename: Yong-jie
authors.initials: YJ
authors.lastname: Xia
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Shen
authors.forename: Meng
authors.initials: M
authors.lastname: An
authors.forename: Yu-hua
authors.initials: YH
authors.lastname: Qiu
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005690:Galactose / Q000633:toxicity*; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008213:Lymphocyte Activation; D051379:Mice; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*
chemical_list: D015847:Interleukin-4; D007371:Interferon-gamma; D005690:Galactose