title: [Analysis on changes of purgative biopotency in different processed products of rhubarb].
abstract: OBJECTIVE
  To determine the difference on purgative biopotency of different processed products of rhubarb and compare rhubarb before and after preparation.
  
  
  METHODS
  The prime biopotency of rhubarb reference substance was determined by comparing with the control substance of sennoside B using rat purgative model. Comparing with calibrated rhubarb reference substance, the bioactivity different processed products of rhubarb was determined by the 2,2',2" method.
  
  
  RESULTS
  The purgative biopotency of crude rhubarb was 825.22 U x g(-1). The purgative biopotency of alcohol-processed rhubarb was 699.05 U x g(-1), The purgative biopotency of steamed rhubarb were 459.76 U x g(-1). Carbonized rhubarb cannot be determinate.
  
  
  CONCLUSIONS
  Crude rhubarb showed a significantly declineing purgative biopotency after being processed, alcohol-processed rhubarb showed less than crude rhubarb and followed by steamed rhubarb. Carbonized rhubarb lost almost all purgative bioactivity.
authors.affiliation: Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Li
authors.forename: Jiabo
authors.initials: J
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Qu
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002400:Cathartics / Q000008:administration & dosage / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D012250:Rheum / Q000737:chemistry*; D012676:Senna Extract / Q000494:pharmacology; D000081226:Sennosides
chemical_list: D002400:Cathartics; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D000081226:Sennosides; D012676:Senna Extract