title: The effects of siRNA against RPL22 on ET-1-induced proliferation of             human pulmonary arterial smooth muscle cells.
abstract: The aim of this study was to investigate the effects of small interference RNA (siRNA) against ribosomal protein L22 (RPL22) on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells (HPASMCs). HPASMCs were transfected with siRNA against RPL22, incubated in smooth muscle cell culture medium (SMCM) and ET-1. RPL22 gene expression and protein levels of HPASMCs were measured by real-time PCR and western blotting, respectively. PCNA, CCK-8 immunohistochemistry and flow cytometry analysis were used to evaluate HPASMC proliferation. Cyclin D1 (CCND1) expression was also assayed. Following transfection with siRNA against RPL22, RPL22 expression was significantly inhibited in the control and ET-1 groups. HPASMC proliferation was significantly suppressed by transfection with siRNA against RPL22. Moreover, CCND1 was also downregulated by inhibiting RPL22 expression. In conclusion, these data suggest that inhibition of RPL22 expression can suppress HPASMC proliferation and CCND1 expression. The effects of siRNA against RPL22 on HPASMC proliferation are considered to be mediated through inhibition of CCND1 expression.
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital                     of Nanjing Medical University, Nanjing 210029, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Xue
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiang-Rong
authors.initials: XR
authors.lastname: Zuo
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Xie
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
mesh_terms: D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D019332:Endothelin-1 / Q000494:pharmacology*; D015870:Gene Expression; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D011651:Pulmonary Artery / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D019332:Endothelin-1; D018809:Proliferating Cell Nuclear Antigen; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; C067169:RPL22 protein, human; D019938:Cyclin D1