title: [Protective effect and mechanism of curcumin on ActD/TNF-α-induced synergistically apoptosis in PC12 cells].
abstract: OBJECTIVE
  To investigate the protective effect and mechanism of curcumin against ActD/TNF-α-induced synergistically apoptosis in PC12 cells.
  
  
  METHODS
  MTT assay was used to evaluate the optimal concentration of drugs; Hoechst 33258 fluorescent staining to observe apoptosis of PC12 cells, JC-1 was used to detect the mitochondrial membrane potential (MMP), real-time PCR was used to detect the expression of two apoptotic genes: Bcl-1 and Bax.
  
  
  RESULTS
  ActD/TNF-α can synergistically reduce viability of PC12 cells(P<0.05), increase the number of cells with pyknosis and karyorrhexis, increase apoptotic rate of cells (P<0.05), decrease MMP in cells, and downregulate expression of Bcl-2(P<0.05). After treating with curcumin(5 μmol/L), survival of PC12 cells was increased(P<0.05), the number of cells with pyknosis and karyorrhexis was reduced, MMP and expression of Bcl-2 were increased(P<0.05).
  
  
  CONCLUSIONS
  Curcumin can resist the ActD/TNF-α-induced synergistically apoptosis in PC12 cells, the mechanisms of which may be related to an increase in MMP and Bcl-2 expression.
authors.affiliation: Medical College of Jinan University, Guangzhou, China.
authors.forename: Jun-bing
authors.initials: JB
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Gong
authors.forename: Jun
authors.initials: J
authors.lastname: Dong
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Xing
authors.forename: Sai
authors.initials: S
authors.lastname: Xie
authors.forename: Qin-ying
authors.initials: QY
authors.lastname: Deng
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Tang
authors.forename: Rui
authors.initials: R
authors.lastname: Pan
authors.forename: Guo-yin
authors.initials: GY
authors.lastname: Xiong
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D003474:Curcumin / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000032:analysis
chemical_list: C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D014409:Tumor Necrosis Factor-alpha; D051028:bcl-2-Associated X Protein; D003609:Dactinomycin; D003474:Curcumin