title: Nuclear organization of cholinergic, putative catecholaminergic, serotonergic and orexinergic systems in the brain of the African pygmy mouse (Mus minutoides): organizational complexity is preserved in small brains.
abstract: This study investigated the nuclear organization of four immunohistochemically identifiable neural systems (cholinergic, catecholaminergic, serotonergic and orexinergic) within the brain of the African pygmy mouse (Mus minutoides). The African pygmy mice studied had a brain mass of around 275 mg, making these the smallest rodent brains to date in which these neural systems have been investigated. In contrast to the assumption that in this small brain there would be fewer subdivisions of these neural systems, we found that all nuclei generally observed for these systems in other rodent brains were also present in the brain of the African pygmy mouse. As with other rodents previously studied in the subfamily Murinae, we observed the presence of cortical cholinergic neurons and a compactly organized locus coeruleus. These two features of these systems have not been observed in the non-Murinae rodents studied to date. Thus, the African pygmy mouse displays what might be considered a typical Murinae brain organization, and despite its small size, the brain does not appear to be any less complexly organized than other rodent brains, even those that are over 100 times larger such as the Cape porcupine brain. The results are consistent with the notion that changes in brain size do not affect the evolution of nuclear organization of complex neural systems. Thus, species belonging to the same order generally have the same number and complement of the subdivisions, or nuclei, of specific neural systems despite differences in brain size, phenotype or time since evolutionary divergence.
authors.affiliation: School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown 2193, Johannesburg, South Africa.
authors.forename: Jean-Leigh
authors.initials: JL
authors.lastname: Kruger
authors.forename: Nina
authors.initials: N
authors.lastname: Patzke
authors.forename: Kjell
authors.initials: K
authors.lastname: Fuxe
authors.forename: Nigel C
authors.initials: NC
authors.lastname: Bennett
authors.forename: Paul R
authors.initials: PR
authors.lastname: Manger
mesh_terms: D000109:Acetylcholine / Q000502:physiology*; D000818:Animals; D001479:Basal Ganglia / Q000502:physiology / Q000648:ultrastructure; D001921:Brain / Q000033:anatomy & histology* / Q000502:physiology* / Q000648:ultrastructure; D002395:Catecholamines / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000502:physiology / Q000648:ultrastructure; D003342:Corpus Striatum / Q000502:physiology / Q000648:ultrastructure; D004027:Diencephalon / Q000502:physiology / Q000648:ultrastructure; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000502:physiology*; D008297:Male; D008526:Medulla Oblongata / Q000502:physiology / Q000648:ultrastructure; D008636:Mesencephalon / Q000502:physiology / Q000648:ultrastructure; D051379:Mice / Q000502:physiology*; D009479:Neuropeptides / Q000502:physiology*; D000068797:Orexins; D012249:Rhombencephalon / Q000502:physiology / Q000648:ultrastructure; D012701:Serotonin / Q000502:physiology*
chemical_list: D002395:Catecholamines; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins; D012701:Serotonin; D000109:Acetylcholine