title: [Decreased mRNA expressions of T-type channel alpha1H and alpha1G in the sperm of varicocele patients and their implication].
abstract: OBJECTIVE
  To study the relationship of varicocele (VC) with the expressions of T-type channel alpha1H and alpha1G in the sperm of VC patients.
  
  
  METHODS
  Based on the WHO criteria, we examined the semen samples by computer-aided sperm analysis (CASA), and divided the samples into groups A (normal semen from volunteers, n = 20), B (normal semen from VC patients, n = 16) and C (abnormal semen from VC patients, n = 44). We optimized the semen by discontinuous Percoll grade centrifugation, and determined the mRNA expressions of T-type channel alpha1H and alpha1G in the three groups using using reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Compared with group A, the mRNA expressions of alpha1H and alpha1G showed with no significant decrease in group B (P>0.05), but were remarkably reduced in group C (P<0.01).
  
  
  CONCLUSIONS
  The abnormal mRNA expressions of T-type channel alpha1H and alpha1G may be one of the causes of declined semen quality and consequently infertility in VC patients, which has pointed out a new direction for the studies of the causes and treatment of VC-related infertility.
authors.affiliation: Department of Urology, Laiwu People's Hospital, Laiwu, Shandong 271100, China. shipingwang719@163.com
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Wang
authors.forename: Pei-Tao
authors.initials: PT
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Zhong-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Xiu-Yan
authors.initials: XY
authors.lastname: Fu
authors.forename: Hua-Qin
authors.initials: HQ
authors.lastname: Li
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D020747:Calcium Channels, T-Type / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D006801:Humans; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D055101:Semen Analysis; D013094:Spermatozoa / Q000378:metabolism*; D014646:Varicocele / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: C121855:CACNA1G protein, human; C479952:CACNA1H protein, human; D020747:Calcium Channels, T-Type; D012333:RNA, Messenger