title: Molecular and kinetic characterization of histamine transport into adult rat cultured astrocytes.
abstract: Astrocytes have a key role in the clearance and inactivation of histamine in the adult central nervous system, but transporters which mediate histamine uptake into astrocytes have not been fully characterized. We therefore investigated the kinetic and molecular characteristics of histamine uptake into cultured adult rat astrocytes. [(3)H]-histamine was taken up by astrocytes in a temperature-, time- and concentration-dependent manner and was inhibited up to 60-70% by 1mM ouabain or by substitution of NaCl with choline chloride. Specific [(3)H]-histamine uptake, determined as the difference between transport at 37 and 4°C, displayed saturation kinetics with the apparent Michaelis-Menten constant (K(m)) of 141 and 101μM and the apparent maximal uptake rate (V(max)) of 22.5 and 17.8pmol/min/mg protein, as estimated from the Woolf and the Eadie-Hofstee plots, respectively. Since our data suggested the presence of a carrier-operated histamine uptake system, we assessed the possible involvement of the organic cation transporters (OCT) 1, 2 and 3, which have been previously described to play a role in histamine transport in the central nervous system. Low level mRNA expression of all OCT isoforms was detected, but in contrast to rat brain cortex homogenate, where OCT3 was the most prominently expressed OCT isoform, OCT2 mRNA was the predominant OCT species in cultured astrocytes. However, OCT inhibitors corticosterone and decynium 22 (D22) had no effect or only modestly reduced [(3)H]-histamine uptake. Thus, our data indicate that adult rat astrocytes possess an efficient high-capacity, low-affinity carrier-operated histamine uptake system, which does not seem to involve OCTs.
authors.affiliation: Department of Pharmacology and Experimental Toxicology, Faculty of Medicine, University of Ljubljana, Korytkova 2, Si-1001 Ljubljana, Slovenia. katja.perdan@mf.uni-lj.si
authors.forename: Katja
authors.initials: K
authors.lastname: Perdan-Pirkmajer
authors.forename: Sergej
authors.initials: S
authors.lastname: Pirkmajer
authors.forename: Katarina
authors.initials: K
authors.lastname: Černe
authors.forename: Mojca
authors.initials: M
authors.lastname: Kržan
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001692:Biological Transport; D002478:Cells, Cultured; D006632:Histamine / Q000378:metabolism*; D007700:Kinetics; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D013696:Temperature
chemical_list: D006632:Histamine