title: PNA HyBeacons for analysis of human mutations related to statin-induced myopathy.
abstract: Aminoalkyl and alkyne-tagged PNA HyBeacons have been synthesized, labeled with fluorescein via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA HyBeacons gave much better mismatch/mutant discrimination than conventional DNA HyBeacons but smaller fluorescence changes on melting.
authors.affiliation: School of Chemistry, University of Southampton, Southampton, UK.
authors.forename: Nittaya
authors.initials: N
authors.lastname: Gale
authors.forename: Petr
authors.initials: P
authors.lastname: Kocalka
authors.forename: Charlotte
authors.initials: C
authors.lastname: Mardle
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D057930:Click Chemistry; D004252:DNA Mutational Analysis / Q000379:methods*; D005456:Fluorescent Dyes; D060005:Genotyping Techniques / Q000379:methods; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000009:adverse effects*; D013058:Mass Spectrometry; D009135:Muscular Diseases / Q000139:chemically induced* / Q000235:genetics*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D044366:Transition Temperature
chemical_list: D005456:Fluorescent Dyes; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D015345:Oligonucleotide Probes; D020135:Peptide Nucleic Acids