title: Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion.
abstract: Riboflavin binding protein (RfBP) is a minor protein in hen egg; its potential involvement in egg allergy has seldom been studied. The aim of this work was to investigate the IgE binding capacity of RfBP before and after simulated gastrointestinal digestion. It was shown that digestion of RfBP mainly occurred during the gastric phase. The protein fragments resulting from the subsequent duodenal phase remained linked through disulfide bonds. Both the intact protein and its digests were subjected to inhibition ELISA with sera obtained from patients allergic to egg. The results revealed significant IgE binding to intact RfBP, whereas the digests showed reduced but substantial IgE binding levels, with serum-to-serum variability. The RfBP digests were then subjected to immunoblot with allergic patients' sera, and the IgE-reactive peptides were further analyzed by MALDI-TOF/TOF mass spectrometry for sequence determination. The RfBP sequence 41-84 was identified as a novel IgE binding peptide in patients allergic to egg.
authors.affiliation: Institute of Food Science Research,  CIAL (UAM-CSIC) , Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Martos
authors.forename: Carlos
authors.initials: C
authors.lastname: Pineda-Vadillo
authors.forename: Beatriz
authors.initials: B
authors.lastname: Miralles
authors.forename: Elena
authors.initials: E
authors.lastname: Alonso-Lebrero
authors.forename: Rosina
authors.initials: R
authors.lastname: López-Fandiño
authors.forename: Elena
authors.initials: E
authors.lastname: Molina
authors.forename: Josefina
authors.initials: J
authors.lastname: Belloque
mesh_terms: D000485:Allergens / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D004063:Digestion; D021181:Egg Hypersensitivity / Q000097:blood / Q000276:immunology; D004527:Egg Proteins / Q000737:chemistry*; D004531:Eggs / Q000032:analysis; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology* / Q000378:metabolism
chemical_list: D000485:Allergens; D004527:Egg Proteins; D026901:Membrane Transport Proteins; D010455:Peptides; C019079:riboflavin-binding protein; D007073:Immunoglobulin E