title: Assessment of the mosaic structure in the Helicobacter pylori cagA gene 3'-region using an improved polymerase chain reaction-based assay.
abstract: The mosaic structure of the cagA gene has been suggested to affect Helicobacter pylori CagA-associated pathogenesis. An improved polymerase chain reaction assay allowed for a rapid and detailed molecular analysis of the cagA gene 3'-region in a single amplification step, followed by amplicon sequencing using universal M13 and T7 sequencing primers.
authors.affiliation: Division of Clinical and Experimental Medicine, Department of Clinical Microbiology, Faculty of Health Sciences, Linköping University, County Council of Östergötland, Linköping, Sweden. hans-jurg.monstein@liu.se
authors.forename: Hans-Jürg
authors.initials: HJ
authors.lastname: Monstein
authors.forename: Anna
authors.initials: A
authors.lastname: Ryberg
authors.forename: Anneli
authors.initials: A
authors.lastname: Karlsson
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; C080866:cagA protein, Helicobacter pylori