title: [Study on separation and analysis of anticoagulant compounds for anticoagulant activity in vitro on mixture of peptide from pepsin enzymolysis of centipede].
abstract: OBJECTIVE
  To separate anticoagulant components from the pepsin enzymolysis of centipede by gel filtration and reverse-phase C18 chromatography, and to detect the distribution range of their molecular mass.
  
  
  METHODS
  Cingula and 280 nm ultraviolet spectrometry were used to detect and collect the chromatographic solutions. The components' anticoagulant activity in vitro was detected with activated partial thromboplastin time (APTT) as the index, and the molecular mass range of the active composition was detected by MALDI-TOF-MS.
  
  
  RESULTS
  Anticoagulant active compounds were produced by gel filtration and reverse-phase C18 chromatography. The distribution range of relative molecular mass was determined to be from 597 to 1 146.
  
  
  CONCLUSIONS
  Gel filtration and reverse-phase C18 chromatography are feasible for separating and purifying the pepsin enzymolysis of Centipede. The anticoagulant active compounds are oligopeptides.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China. jerry858789@163.com
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Yurong
authors.initials: Y
authors.lastname: Wang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhao
authors.forename: Nengting
authors.initials: N
authors.lastname: Huang
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000302:isolation & purification* / Q000378:metabolism*; D001181:Arthropods / Q000737:chemistry* / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D008297:Male; D010314:Partial Thromboplastin Time; D010434:Pepsin A / Q000737:chemistry* / Q000378:metabolism*; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism*; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000925:Anticoagulants; D010455:Peptides; D010434:Pepsin A