title: Comparison of the simple cyclic voltammetry (CV) and DPPH assays for the determination of antioxidant capacity of active principles.
abstract: Antioxidant activity of a number of small (low molecular weight) natural compounds found in spices, condiments or drugs (gallic acid, sesamol, eugenol, thymol, carvacrol, vanillin, salicylaldehyde, limonene, geraniol, 4-hexylresorcinol, etc.) has been evaluated using electrochemical and DPPH• radical scavenging measurements. Structural analysis of the tested compound suggest a remarkable activity for phenol derivatives and the importance of the -R groups located on the phenolic ring in the molecule's ability to act as free radical scavenging as well as their influence in the electrochemical behavior. The voltammetric method can be used for the determination of the antioxidant capability in the same manner as the DPPH• radical scavenging because of the correlation found between oxidation potentials and anti-radical power (ARP = 1/EC₅₀). Such electrochemical determination is fast and cheap and allows making measurements under a variety of experimental conditions. The accuracy of the electrochemical measurements is the same for all the compounds, irrespective of their scavenging activity, the opposite of what occurs in the DPPH• test.
authors.affiliation: Department of Chemical Engineering, Physical Chemistry and Organic Chemistry, University of Huelva, Campus de El Carmen, 21071 Huelva, Spain. jesus.fernandez@diq.uhu.es
authors.forename: Jesús F
authors.initials: JF
authors.lastname: Arteaga
authors.forename: Mercedes
authors.initials: M
authors.lastname: Ruiz-Montoya
authors.forename: Alberto
authors.initials: A
authors.lastname: Palma
authors.forename: Gema
authors.initials: G
authors.lastname: Alonso-Garrido
authors.forename: Sara
authors.initials: S
authors.lastname: Pintado
authors.forename: José M
authors.initials: JM
authors.lastname: Rodríguez-Mellado
mesh_terms: D000080462:Acyclic Monoterpenes; D000447:Aldehydes / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry*; D001547:Benzaldehydes / Q000737:chemistry; D052117:Benzodioxoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors*; D003212:Condiments; D053138:Cyclohexenes / Q000737:chemistry; D000081007:Cymenes; D055664:Electrochemical Techniques; D005054:Eugenol / Q000737:chemistry; D005609:Free Radicals / Q000037:antagonists & inhibitors*; D005707:Gallic Acid / Q000737:chemistry; D006586:Hexanes / Q000737:chemistry; D006604:Hexylresorcinol; D000077222:Limonene; D039821:Monoterpenes / Q000737:chemistry; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry; D010851:Picrates / Q000037:antagonists & inhibitors*; D055553:Prescription Drugs; D012118:Resorcinols / Q000737:chemistry; D017365:Spices; D013729:Terpenes / Q000737:chemistry; D013943:Thymol / Q000737:chemistry
chemical_list: D000080462:Acyclic Monoterpenes; D000447:Aldehydes; D000975:Antioxidants; D001547:Benzaldehydes; D052117:Benzodioxoles; D001713:Biphenyl Compounds; D053138:Cyclohexenes; D000081007:Cymenes; D005609:Free Radicals; D006586:Hexanes; D039821:Monoterpenes; D010636:Phenols; D010851:Picrates; D055553:Prescription Drugs; D012118:Resorcinols; D013729:Terpenes; C013243:salicylaldehyde; D013943:Thymol; D005054:Eugenol; D005707:Gallic Acid; C025583:sesamol; C073316:carvacrol; D000077222:Limonene; C100058:vanillin; C004931:1,1-diphenyl-2-picrylhydrazyl; C007836:geraniol; D006604:Hexylresorcinol