title: Impact of hepatic arterial reconstruction on orthotopic liver transplantation in the rat.
abstract: Orthotopic liver transplantation (OLT) models in rats have been investigated in many studies, but detailed information on the impact of hepatic artery (HA) reconstruction on postoperative factors remains to be investigated. HA reconstruction also requires advanced skills. The effect of the reconstruction of the HA by a hand-suture technique in rats with a whole-liver syngeneic graft was investigated. Long-term survival, histopathological assessment, immunohistological evaluation, and blood biochemistry were investigated until postoperative day (POD) 28. From the early postoperative period, significant differences between OLTs with or without HA reconstruction were found in graft parenchymal damage, induction of apoptosis, and transaminase levels, though survival curves and the coagulation profile showed no differences. In OLT without HA reconstruction, biliary proliferation was decreased at POD 5-14, and total bilirubin level was increased at PODs 10 and 14. The study indicates that HA reconstruction is required for reliable OLT in rats.
authors.affiliation: Department of Neuroscience, Mayo Clinic, Jacksonville, Florida, USA. hori.tomohide@mayo.edu
authors.forename: Tomohide
authors.initials: T
authors.lastname: Hori
authors.forename: Lindsay B
authors.initials: LB
authors.lastname: Gardner
authors.forename: Florence
authors.initials: F
authors.lastname: Chen
authors.forename: Ann-Marie T
authors.initials: AM
authors.lastname: Baine
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Hata
authors.forename: Shinji
authors.initials: S
authors.lastname: Uemoto
authors.forename: Justin H
authors.initials: JH
authors.lastname: Nguyen
mesh_terms: D000818:Animals; D017209:Apoptosis; D001652:Bile Ducts / Q000473:pathology; D001777:Blood Coagulation; D006499:Hepatic Artery / Q000601:surgery*; D008099:Liver / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D019651:Plastic Surgery Procedures