title: The bactericidal and biocompatible characteristics of reinforced calcium phosphate cements.
abstract: Infection remains a serious medical problem in orthopaedic surgery. Antibiotic administration can be available either systemically via the blood stream or locally, directly into the infected bone. One of the main limitations of antibiotic administration is the development of multi-antibiotic-resistant bacterial strains. In this study, we developed bactericidal calcium phosphate cements (CPC) by incorporation of different molecular weight chitosan and hydroxypropyltrimethyl ammonium chloride chitosan (HACC). Two standard strains, S. epidermidis (ATCC35984) and S. aureus (ATCC25923), and one clinical isolate, methicillin-resistant S. epidermidis (MRSE), were selected to evaluate the antibacterial activity of these bone cements. Our data showed that the CPC loaded with low molecular weight chitosan and HACC significantly inhibited the bacterial adhesion and biofilm formation. In addition, HACC-containing CPC has no cytotoxic effects on both mouse pluripotent C3H10T1/2cell line and a murine L929 fibroblast cell line. We propose that HACC-containing CPC represents a promising polymer-based bactericidal bone scaffold in controlling orthopaedic surgery-related infection.
authors.affiliation: Department of Orthopaedic Surgery, 2nd Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing 210011, People's Republic of China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Wu
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Hua
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: He
authors.forename: Xinfu
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Yu
authors.forename: Weiping
authors.initials: W
authors.lastname: Ren
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry*; D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000737:chemistry*; D001843:Bone Cements / Q000737:chemistry / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D048271:Chitosan / Q000031:analogs & derivatives / Q000737:chemistry; D015195:Drug Design; D005347:Fibroblasts / Q000166:cytology; D051379:Mice; D008809:Mice, Inbred C3H; D018613:Microscopy, Confocal / Q000379:methods; D008970:Molecular Weight; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; D000644:Quaternary Ammonium Compounds; C576941:hydroxypropyltrimethyl ammonium chloride chitosan; D048271:Chitosan