title: Genetic incorporation of D-lysine into diketoreductase in Escherichia coli cells.
abstract: Pyrococcus horikoshii lysyl-tRNA synthetase/tRNA orthogonal pair exhibited high selectivity towards D-lysine in the presence of excess amount of D-lysine. Based on the observation, this orthogonal pair was employed to encode D-lysine, and D-lysine was site-specifically incorporated into the diketoreductase in E. coli cells.
authors.affiliation: Laboratory of Chemical Biology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tongjia Street, Nanjing, 210009, Jiangsu, People's Republic of China.
authors.forename: Zhizhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.forename: Denghuan
authors.initials: D
authors.lastname: Yi
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Wang
authors.forename: Yijun
authors.initials: Y
authors.lastname: Chen
mesh_terms: D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D008239:Lysine / Q000235:genetics*; D008958:Models, Molecular; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism
chemical_list: D010088:Oxidoreductases; D008239:Lysine