title: Effects of SEMA3G on migration and invasion of glioma cells.
abstract: Glioblastoma multiforme is the most aggressive type of brain tumor with             a strong ability to invade and migrate into surrounding normal brain tissues,             leading to high tumor recurrence and mortality. Most of class-3 semaphorins, especially             SEMA3A, SEMA3B and SEMA3F, have been reported to have strong tumor inhibition             ability, but the role of SEMA3G in tumor biology is largely unknown. We report             here that SEMA3G possesses anti-migration and anti-invasion ability. To determine             the potential effects of SEMA3G on migratory and invasive ability, we generated             stable SEMA3G expression U251MG cells. We found that stably overexpressed SEMA3G             inhibited the migratory and invasive behavior of U251MG cells. In addition, treatment             with SEMA3G conditioned media also decreased the migratory and invasive ability             of parental U251MG cells. Furthermore, SEMA3G also inhibited the activity of MMP2,             an index of tumor invasion ability. Thus, our results suggest that SEMA3G inhibited             tumor cell migration and invasion, which may be obtained through cell autonomous             or paracrine mechanisms, and SEMA3G is a potential target for antitumor migration             and invasion.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, PR China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Jia
authors.initials: J
authors.lastname: Gu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Shi
authors.forename: Rutong
authors.initials: R
authors.lastname: Yu
mesh_terms: D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D017077:Culture Media, Conditioned; D015870:Gene Expression; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned; D011994:Recombinant Proteins; D039961:Semaphorins; C569384:semaphorin 3G, human; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2