title: Charge-conversional poly(amino acid)s derivatives as a drug delivery carrier in response to the tumor environment.
abstract: A charge-converting and pH-dependent nanocarrier was achieved by conjugating 2,3-dimethylmaleic anhydride (DMMA) to the amino group of an octadecyl grafted poly (2-hydroxyethyl aspartamide) (PHEA-g-C(18)-NH(2)) backbone, thereby forming a spherical micelle. PHEA, a poly(amino acid)s derivative, was derived from poly(succinimide), which is biocompatible and biodegradable. DMMA, a detachable component at the tumor site, was added, preventing aggregation with negative blood serum and enhancing the nanocarrier's cellular uptake. The polymeric micelle was comprehensively characterized and doxorubicin was encapsulated successively. The cellular uptake and anticancer therapeutic effect were evaluated by flow cytometry, confocal laser scanning microscopy, and a MTT assay. The properties of the nanocarrier can further be exploited to develop an early detection module for cancer. The present work is also expected to advance the study of designing smart carriers for drug and gene delivery.
authors.affiliation: Department of Chemical and Biomolecular Engineering (BK21 Graduate Program), Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Se Rim
authors.initials: SR
authors.lastname: Yoon
authors.forename: Hee-Man
authors.initials: HM
authors.lastname: Yang
authors.forename: Chan Woo
authors.initials: CW
authors.lastname: Park
authors.forename: Sujin
authors.initials: S
authors.lastname: Lim
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
authors.forename: Jong-Duk
authors.initials: JD
authors.lastname: Kim
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008299:Maleic Anhydrides / Q000737:chemistry; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D012542:Scattering, Radiation; D059016:Tumor Microenvironment / Q000187:drug effects
chemical_list: D000596:Amino Acids; D004337:Drug Carriers; D008299:Maleic Anhydrides; D011108:Polymers; C007474:2,3-dimethylmaleic anhydride; D004317:Doxorubicin