title: Validation of methylation-sensitive high resolution melting for the detection of DNA methylation in cholangiocarcinoma.
abstract: OBJECTIVE
  To validate methylation-sensitive high resolution melting (MS-HRM) for detection of DNA methylation.
  
  
  METHODS
  Methylation of two independent loci, OPCML and DcR1, was analyzed in cholangiocarcinoma and adjacent normal samples by using MS-HRM, methylation-specific PCR and pyrosequencing.
  
  
  RESULTS
  There was significant agreement between methods at both loci.
  
  
  CONCLUSIONS
  MS-HRM represents the excellent potential and reliability for quantifying DNA methylation levels in clinical samples.
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Rattaya
authors.initials: R
authors.lastname: Amornpisutt
authors.forename: Ruethairat
authors.initials: R
authors.lastname: Sriraksa
authors.forename: Temduang
authors.initials: T
authors.lastname: Limpaiboon
mesh_terms: D001650:Bile Duct Neoplasms / Q000737:chemistry / Q000235:genetics; D001653:Bile Ducts, Intrahepatic / Q000737:chemistry; D015815:Cell Adhesion Molecules / Q000235:genetics; D018281:Cholangiocarcinoma / Q000737:chemistry / Q000235:genetics*; D018899:CpG Islands; D019175:DNA Methylation; D058851:GPI-Linked Proteins / Q000737:chemistry / Q000235:genetics; D056426:Genetic Loci; D015894:Genome, Human; D006801:Humans; D016014:Linear Models; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods*; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D053319:Tumor Necrosis Factor Decoy Receptors / Q000737:chemistry / Q000235:genetics
chemical_list: D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; C059785:OPCML protein, human; D053320:Receptors, Tumor Necrosis Factor, Member 10c; C108661:TNFRSF10C protein, human; D053319:Tumor Necrosis Factor Decoy Receptors