title: Preparation and characterization of caffeic acid-grafted electrospun poly(L-lactic acid) fiber mats for biomedical applications.
abstract: Caffeic acid (CA) was chemically immobilized onto the surfaces of the individual electrospun poly(l-lactic acid) (PLLA) fibers to enhance the hydrophilicity and impart the antioxidant activity to the obtained fibrous membranes. This was done in two sequential steps. First, amino groups were covalently introduced onto the surfaces through the reaction with 1,6-hexamethylenediamine (HMD). In the second step, the amino moieties reacted with CA, which had been preactivated sequentially with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The success of the reactions was confirmed by the ninhydrin assay and X-ray photoelectron spectroscopic analysis (XPS). Indirect cytotoxicity evaluation with murine dermal fibroblasts (L929) and human dermal fibroblasts (HDFa) revealed that the neat and the modified PLLA fibrous matrices released no substances in the levels that were harmful to the cells. Direct culturing of HDFa on these fibrous substrates indicated that they supported the proliferation of the cells on days 2 and 3 very well and that the CA-immobilized substrates exhibited the highest cell viability. Lastly, the antioxidant activity of the CA-immobilized substrates, as revealed by the 1,1-diphenyl-2-picryldrazyl (DPPH) radical scavenging assay, was as high as 88% on average.
authors.affiliation: The Petroleum Petrochemical College, The Center of Excellence on Petrochemical and Materials Technology, ‡Department of Anatomy, Faculty of Dentistry, Chulalongkorn University , Bangkok 10330, Thailand.
authors.forename: Piyachat
authors.initials: P
authors.lastname: Chuysinuan
authors.forename: Prasit
authors.initials: P
authors.lastname: Pavasant
authors.forename: Pitt
authors.initials: P
authors.lastname: Supaphol
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002109:Caffeic Acids / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003959:Diamines / Q000737:chemistry; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D013388:Succinimides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D003959:Diamines; D011091:Polyesters; D011108:Polymers; D013388:Succinimides; D019344:Lactic Acid; C033616:poly(lactide); C001426:N-hydroxysuccinimide; D005022:Ethyldimethylaminopropyl Carbodiimide; C007297:1,6-diaminohexane