title: Effect of basic fibroblast growth factor on cat corneal endothelial cell proliferation.
abstract: OBJECTIVE
  To investigate the function of basic fibroblast growth factor (bFGF) on cat corneal endothelial cells proliferation.
  
  
  METHODS
  Cat corneal endothelial cells were primarily cultured, stimulated with bFGF for different period, the proliferation of cells was assayed by modified tertrozalium salt (MTT) method, and the morphologic changes were observed with inverted phase contrast microscope and transmission electron microscope.
  
  
  RESULTS
  At 1, 3 and 5 days after bFGF was added to cat corneal endothelial cells, the result of MTT in 490nm showed significant difference than that in control group, and the difference was most significant in 10ng/mL group.
  
  
  CONCLUSIONS
  bFGF can promote proliferation of cat corneal endothelial cells. 10ng/mL is the relatively most effective dose.
authors.affiliation: Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Luo
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Mei-Guang
authors.initials: MG
authors.lastname: Liu
authors.forename: Chuan-Fu
authors.initials: CF
authors.lastname: Wang