title: The OPFOS Microscopy Family: High-Resolution Optical Sectioning of Biomedical Specimens.
abstract: We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light. Fluorescence originating in the cross-section of the sheet and sample is recorded orthogonally with a camera. In this paper, the first implementation of LSFM to image biomedical tissue in three dimensions-orthogonal-plane fluorescence optical sectioning microscopy (OPFOS)-is discussed. Since then many similar and derived methods have surfaced, (SPIM, ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM, etc.) which we all briefly discuss. All these optical sectioning methods create images showing histological detail. We illustrate the applicability of LSFM on several specimen types with application in biomedical and life sciences.
authors.affiliation: Laboratory of Biomedical Physics, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
authors.forename: Jan A N
authors.initials: JA
authors.lastname: Buytaert
authors.forename: Emilie
authors.initials: E
authors.lastname: Descamps
authors.forename: Dominique
authors.initials: D
authors.lastname: Adriaens
authors.forename: Joris J J
authors.initials: JJ
authors.lastname: Dirckx