title: Epigallocatechin-3-gallate Inhibits LPS-Induced NF-κB and MAPK Signaling Pathways in Bone Marrow-Derived Macrophages.
abstract: OBJECTIVE
  Epigallocatechin-3-gallate (EGCG), the primary catechin in green tea, has anti-inflammatory and anti-oxidative properties. The aim of the current study was to characterize the impact of EGCG on lipopolysaccharide (LPS)-induced innate signaling in bone marrow-derived macrophages (BMMs) isolated from ICR mice.
  
  
  METHODS
  The effect of EGCG on LPS-induced pro-inflammatory gene expression and nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling was examined using reverse transcription-polymerase chain reaction, Western blotting, immunofluorescence, and the electrophoretic mobility shift assay.
  
  
  RESULTS
  EGCG inhibited accumulation of LPS-induced IL-12p40, IL-6, MCP-1, ICAM-1, and VCAM-1 mRNA in BMMs. EGCG blocked LPS-induced IκBα degradation and RelA nuclear translocation. EGCG blocked the DNA-binding activity of NF-κB. LPS-induced phosphorylation of ERK1/2, JNK, and p38 was inhibited by EGCG. U0126 (an inhibitor of MEK-1/2) suppressed the LPS-induced IL-12p40, IL-6, MCP-1, ICAM-1, and VCAM-1 mRNA accumulation in BMMs.
  
  
  CONCLUSIONS
  These results indicate that EGCG may prevent LPS-induced pro-inflammatory gene expression through blocking NF-κB and MAPK signaling pathways in BMMs.
authors.affiliation: Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: So-Young
authors.initials: SY
authors.lastname: Joo
authors.forename: Young-A
authors.initials: YA
authors.lastname: Song
authors.forename: Young-Lan
authors.initials: YL
authors.lastname: Park
authors.forename: Eun
authors.initials: E
authors.lastname: Myung
authors.forename: Cho-Yun
authors.initials: CY
authors.lastname: Chung
authors.forename: Kang-Jin
authors.initials: KJ
authors.lastname: Park
authors.forename: Sung-Bum
authors.initials: SB
authors.lastname: Cho
authors.forename: Wan-Sik
authors.initials: WS
authors.lastname: Lee
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Jong-Sun
authors.initials: JS
authors.lastname: Rew
authors.forename: Nack-Sung
authors.initials: NS
authors.lastname: Kim
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Joo