title: A new single nucleotide polymorphism in the ryanodine  gene of chicken skeletal muscle.
abstract: Some genes affect meat quality in chickens. We looked  for polymorphisms in the Gallus gallus α-RyR gene (homologous to RyR 1) that could  be associated with PSE (pale, soft and exudative) meat. Because RyR genes are  over 100,000 bp long and code for proteins with about 5000 amino acids, primers  were designed to amplify a fragment of hotspot region 2, a region with a high  density of mutations in other species. Total blood DNA was extracted from 50  birds, 25 that had PSE meat and 25 normal chickens. The DNA samples were  amplified by PCR, cloned, sequenced, and used to identify single nucleotide  polymorphisms (SNPs). The amplified fragment of α-RyR was 604 nucleotides in length; 181  nucleotides were similar to two exons from a hypothetical turkey cDNA sequence  for α-RyR. A non-synonymous nucleotide  substitution (G/A) was identified in at least one of the three sequenced clones  obtained from nine animals, six PSE (HAL+) birds and three normal (HAL-) birds;  they were heterozygous for this mutation. This SNP causes a change from Val to  Met in the α-RYR protein. Since the frequencies of  this SNP were not significantly different in the PSE versus normal chickens, it  appears that this mutation (in heterozygosity) does not alter the structure or  function of the muscle protein, making it an inappropriate candidate as a  genetic marker for PSE meat.
authors.affiliation: Departamento de Ciência e Tecnologia de Alimentos, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: A A
authors.initials: AA
authors.lastname: Droval
authors.forename: E
authors.initials: E
authors.lastname: Binneck
authors.forename: S R R
authors.initials: SR
authors.lastname: Marin
authors.forename: F G
authors.initials: FG
authors.lastname: Paião
authors.forename: A
authors.initials: A
authors.lastname: Oba
authors.forename: A L
authors.initials: AL
authors.lastname: Nepomuceno
authors.forename: M
authors.initials: M
authors.lastname: Shimokomaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D017931:DNA Primers; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D019837:Ryanodine Receptor Calcium Release Channel