title: Determination of gene expression patterns by in situ hybridization in sections.
abstract: In recent years, in situ RNA hybridization technique has found a widespread application in developmental biology. This method has frequently been used to determine gene expression patterns, which is a first step toward understanding of a gene function. Here, we provide a reliable and sensitive method for in situ RNA hybridization on frozen sections of mouse embryo using digoxigenin-labeled RNA probes. This technique can be used to study gene expression patterns at all stages of odontogenesis.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.
authors.forename: Kateryna
authors.initials: K
authors.lastname: Kyrylkova
authors.forename: Sergiy
authors.initials: S
authors.lastname: Kyryachenko
authors.forename: Chrissa
authors.initials: C
authors.lastname: Kioussi
authors.forename: Mark
authors.initials: M
authors.lastname: Leid
mesh_terms: D000818:Animals; D004076:Digoxigenin / Q000737:chemistry; D004622:Embryo, Mammalian / Q000378:metabolism*; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D015347:RNA Probes / Q000737:chemistry; D014070:Tooth / Q000196:embryology* / Q000378:metabolism*
chemical_list: D015347:RNA Probes; D004076:Digoxigenin