title: A new index for acute rejection after renal transplant: Notch receptor-1.
abstract: OBJECTIVE
  This study aimed at investigating the relation between expression of Notch receptor-1 (Notch 1) in peripheral blood mononuclear cells and acute rejection after renal transplant.
  
  
  METHODS
  Ninety-seven patients receiving a renal transplant were randomly selected. Peripheral blood samples before transplant and days 1, 3, 5, 7, 10, 14, 21, and 30 after transplant were retrospectively observed. Expression of Notch 1 was detected by flow cytometry and real-time quantitative polymerase chain reaction.
  
  
  RESULTS
  Expression of Notch 1 was correlated with acute rejection and long-term renal function after transplant (as detected by the level of serum creatinine 6 months after transplant). Expression of Notch 1 in peripheral blood mononuclear cells increased before serum creatinine increased. Expression of Notch 1 can reveal the immune state of recipients after transplant, and Notch 1 expression at early time points after transplant can predict long-term renal function.
  
  
  CONCLUSIONS
  Notch 1 can serve as an important index for acute rejection and long-term renal function after transplant.
authors.affiliation: Organ Transplant Institute, Fuzhou General Hospital, No.156 Xi'erhuan North Road, Fuzhou, 350025, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zheng
authors.forename: Xinghui
authors.initials: X
authors.lastname: Sun
authors.forename: Weizhen
authors.initials: W
authors.lastname: Wu
authors.forename: Shunliang
authors.initials: S
authors.lastname: Yang
authors.forename: Jinquan
authors.initials: J
authors.lastname: Cai
authors.forename: Jianming
authors.initials: J
authors.lastname: Tan
mesh_terms: D000208:Acute Disease; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D042241:Early Diagnosis; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006084:Graft Rejection / Q000175:diagnosis* / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000502:physiology; D016030:Kidney Transplantation / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D051881:Receptor, Notch1 / Q000235:genetics* / Q000276:immunology*; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D055815:Young Adult
chemical_list: D015415:Biomarkers; C497761:NOTCH1 protein, human; D012333:RNA, Messenger; D051881:Receptor, Notch1; D003404:Creatinine