title: Reduced Mid1 Expression and Delayed Neuromotor Development in daDREAM Transgenic Mice.
abstract: Downstream regulatory element antagonist modulator (DREAM) is a Ca(2+)-binding protein that binds DNA and represses transcription in a Ca(2+)-dependent manner. Previous work has shown a role for DREAM in cerebellar function regulating the expression of the sodium/calcium exchanger 3 (NCX3) in cerebellar granular neurons to control Ca(2+) homeostasis and survival of these neurons. To achieve a global view of the genes regulated by DREAM in the cerebellum, we performed a genome-wide analysis in transgenic cerebellum expressing a Ca(2+)-insensitive/CREB-independent dominant active mutant DREAM (daDREAM). Here we show that DREAM regulates the expression of the midline 1 (Mid1) gene early after birth. As a consequence, daDREAM mice exhibit a significant shortening of the rostro-caudal axis of the cerebellum and a delay in neuromotor development early after birth. Our results indicate a role for DREAM in cerebellar function.
authors.affiliation: Genomic Regulation Center, Parc de Recerca Biomèdica de Barcelona, Centro de Investigación Biomédica en Red de Enfermedades Raras Barcelona, Spain.
authors.forename: Mara
authors.initials: M
authors.lastname: Dierssen
authors.forename: Laura
authors.initials: L
authors.lastname: Fedrizzi
authors.forename: Rosa
authors.initials: R
authors.lastname: Gomez-Villafuertes
authors.forename: María Martinez
authors.initials: MM
authors.lastname: de Lagran
authors.forename: Alfonso
authors.initials: A
authors.lastname: Gutierrez-Adan
authors.forename: Ignasi
authors.initials: I
authors.lastname: Sahún
authors.forename: Belen
authors.initials: B
authors.lastname: Pintado
authors.forename: Juan C
authors.initials: JC
authors.lastname: Oliveros
authors.forename: Xose M
authors.initials: XM
authors.lastname: Dopazo
authors.forename: Paz
authors.initials: P
authors.lastname: Gonzalez
authors.forename: Marisa
authors.initials: M
authors.lastname: Brini
authors.forename: Britt
authors.initials: B
authors.lastname: Mellström
authors.forename: Ernesto
authors.initials: E
authors.lastname: Carafoli
authors.forename: Jose R
authors.initials: JR
authors.lastname: Naranjo