title: Lipopolysaccharides induces MUC5AC overproduction in human nasal epithelium.
abstract: Hyperproduction of mucin in the nasal epithelium is an important feature of nasal inflammatory diseases. We investigated the mechanism of lipopolysaccharides (LPS) involvement in mucin 5 subtype AC (MUC5AC) expression in human nasal epithelial cells. The primary human nasal epithelial cells were cultured in vitro, which were treated with LPS (10 nM/ml or 1 μM/ml) for 12 and 24 h. LPS-induced MUC5AC protein was determined in nasal epithelial cells. The levels of nuclear factor kappa B p65 (NF-κBp65) and its inhibitor kappa Bα (IκBα) protein were also detected, and interleukin-1β (IL-1β) mRNA was detected by real-time PCR. LPS up-regulated MUC5AC protein in human nasal epithelial cells, and we determined that the up-regulation of MUC5AC expression was due to a time- and dose-dependent degradation of IκBα protein, which resulted in the increase of NF-κBp65 nuclear translocation. Subsequently, we also determined that LPS can induce IL-1β mRNA in a time- and dose-dependent manner. These data show that LPS treatment activated NF-κB by promoting the degradation of IκBα and the nuclear localization of NF-κBp65, which induced MUC5AC overproduction.
authors.affiliation: Department of Anatomy, China Three Gorges University, Medical College, No.8, Daxue Road, Yichang 443002, Hubei, China. wwwhbqh@163.com
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.forename: Xianyan
authors.initials: X
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Zheng
authors.forename: Lingyan
authors.initials: L
authors.lastname: Wan
mesh_terms: D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008875:Middle Aged; D055271:Mucin 5AC / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D009297:Nasal Mucosa / Q000378:metabolism*; D055815:Young Adult
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D055271:Mucin 5AC; D016328:NF-kappa B