title: Overexpression of the Qc-SNARE gene OsSYP71 enhances tolerance to oxidative stress and resistance to rice blast in rice (Oryza sativa L.).
abstract: OsSYP71 is an oxidative stress and rice blast response gene that encodes a Qc-SNARE protein in rice. Qc-SNARE proteins belong to the superfamily of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), which function as important components of the vesicle trafficking machinery in eukaryotic cells. In this paper, 12 Qc-SNARE genes were isolated from rice, and expression patterns of 9 genes were detected in various tissues and in seedlings challenged with oxidative stresses and inoculated with rice blast. The expression of OsSYP71 was clearly up-regulated under these stresses. Overexpression of OsSYP71 in rice showed more tolerance to oxidative stress and resistance to rice blast than wild-type plants. These results indicate that Qc-SNAREs play an important role in rice response to environmental stresses, and OsSYP71 is useful in engineering crop plants with enhanced tolerance to oxidative stress and resistance to rice blast.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing, China
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Bao
authors.forename: Shu-Jing
authors.initials: SJ
authors.lastname: Sun
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Wen-Lei
authors.initials: WL
authors.lastname: Cao
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.forename: Hai-Juan
authors.initials: HJ
authors.lastname: Tang
authors.forename: Ji
authors.initials: J
authors.lastname: Huang
authors.forename: Zhou-Fei
authors.initials: ZF
authors.lastname: Wang
authors.forename: Jian-Fei
authors.initials: JF
authors.lastname: Wang
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000382:microbiology*; D018384:Oxidative Stress; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D030821:Plants, Genetically Modified; D050600:SNARE Proteins / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D010940:Plant Proteins; D050600:SNARE Proteins