title: Polyoxometalates as peroxidase mimetics and their applications in H2O2 and glucose detection.
abstract: Polyoxometalates (H(3)PW(12)O(40), H(4)SiW(12)O(40) and H(3)PMo(12)O(40)) have been proven to possess intrinsic peroxidase-like activity for the first time, which can catalyze oxidation of the peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) by H(2)O(2) to form a blue color in aqueous solution. Among them, H(3)PW(12)O(40) (PW(12)) exhibits higher catalytic activity to TMB than natural enzyme HRP and other two POMs. In addition, H(3)PW(12)O(40)/graphene exhibited higher activity than H(3)PW(12)O(40) in this catalytic oxidation reaction due to the effect of graphene in promoting the electron transfer between the substrate and catalyst. POMs/H(2)O(2)/TMB system provides a simple, accurate approach to colorimetric detection for H(2)O(2) or glucose. The colorimetric method based on POMs showed good response toward H(2)O(2) and glucose detection with a linear range from 1.34×10(-7) to 6.7×10(-5) mol/L and 1×10(-7) to 1×10(-4) mol/L, respectively. The results showed that it is a simple, cheap, more convenient, highly selective, sensitive, and easy handling colorimetric assay.
authors.affiliation: Key Laboratory of Polyoxometalate Science of Ministry of Education, Faculty of Chemistry, Northeast Normal University, Changchun 130024, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.forename: Dongxue
authors.initials: D
authors.lastname: Han
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Qi
authors.forename: Meisheng
authors.initials: M
authors.lastname: Zhao
mesh_terms: D001560:Benzidines; D032701:Biomimetics; D003124:Colorimetry / Q000379:methods; D005947:Glucose / Q000302:isolation & purification*; D006108:Graphite / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000302:isolation & purification*; D009195:Peroxidase / Q000737:chemistry; D017973:Tungsten Compounds / Q000737:chemistry*; D000099294:Polyoxometalates
chemical_list: D001560:Benzidines; D005947:Glucose; D006108:Graphite; D006861:Hydrogen Peroxide; D009195:Peroxidase; D017973:Tungsten Compounds; C102296:polyoxometalate I; D000099294:Polyoxometalates; C021758:3,3',5,5'-tetramethylbenzidine