title: Cell density-dependent acetylation of ΔNp63α is associated with p53-dependent cell cycle arrest.
abstract: ΔNp63α is a p63 isoform that is predominantly expressed in the epidermal stem cells and in cancer. To find the regulatory pathways of ΔNp63α, we assessed whether ΔNp63α is acetylated and determined the functional implications of acetylation. First, the hinge region of p63 was shown to be acetylated by PCAF, similarly to other p53 family members. Second, acetylation synergistically induced cytoplasmic localization of ΔNp63α. Finally, acetyl-ΔNp63α was induced during high-density culture, suggesting that acetylation of ΔNp63α may reinforce cell cycle arrest upon cell contact. Altogether, these findings suggest that acetylation of ΔNp63α contributes to the epidermal homeostasis.
authors.affiliation: Department of Pathology, Korea University School of Medicine, Seoul, Republic of Korea.
authors.forename: Yang-Seok
authors.initials: YS
authors.lastname: Chae
authors.forename: Hyunji
authors.initials: H
authors.lastname: Kim
authors.forename: Dongsung
authors.initials: D
authors.lastname: Kim
authors.forename: Hyunsook
authors.initials: H
authors.lastname: Lee
authors.forename: Hae-ock
authors.initials: HO
authors.lastname: Lee
mesh_terms: D000107:Acetylation; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; C115051:TP63 protein, human; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins