title: Interkingdom signaling: integration, conformation, and orientation of N-acyl-L-homoserine lactones in supported lipid bilayers.
abstract: N-Acyl-L-homoserine lactones (AHLs) are small cell-to-cell signaling molecules involved in the regulation of population density and local gene expression in microbial communities. Recent evidence shows that contact of this signaling system, usually referred to as quorum sensing, to living eukaryotes results in interactions of AHL with host cells in a process termed "interkingdom signaling". So far details of this process and the binding site of the AHLs remain unknown; both an intracellular and a membrane-bound receptor seem possible, the first of which requires passage through the cell membrane. Here, we used sum-frequency-generation (SFG) spectroscopy to investigate the integration, conformation, orientation, and translocation of deuterated N-acyl-L-homoserine lactones (AHL-d(n)) with varying chain length (8, 12, and 14 C atoms) in lipid bilayers consisting of a 1:1 mixture of POPC:POPG supported on SiO(2) substrates (prepared by vesicle fusion). We found that all AHL-d(n) derivatives are well-ordered within the supported lipid bilayer (SLB) in a preferentially all-trans conformation of the deuterated alkyl chain and integrated into the upper leaflet of the SLB with the methyl terminal groups pointing downward. For the bilayer system described above, no flip-flop of AHL-d(n) from the upper leaflet to the lower one could be observed. Spectral assignments and interpretations were further supported by Fourier transform infrared and Raman spectroscopy.
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Postfach 3640, 76021 Karlsruhe, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Barth
authors.forename: Dorota
authors.initials: D
authors.lastname: Jakubczyk
authors.forename: Adam
authors.initials: A
authors.lastname: Kubas
authors.forename: Frances
authors.initials: F
authors.lastname: Anastassacos
authors.forename: Gerald
authors.initials: G
authors.lastname: Brenner-Weiss
authors.forename: Karin
authors.initials: K
authors.lastname: Fink
authors.forename: Ute
authors.initials: U
authors.lastname: Schepers
authors.forename: Stefan
authors.initials: S
authors.lastname: Bräse
authors.forename: Patrick
authors.initials: P
authors.lastname: Koelsch
mesh_terms: D054742:Acyl-Butyrolactones / Q000737:chemistry*; D003903:Deuterium; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D008968:Molecular Conformation; D010713:Phosphatidylcholines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D053038:Quorum Sensing; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D054742:Acyl-Butyrolactones; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D012822:Silicon Dioxide; C060037:1-palmitoyl-2-oleoylglycero-3-phosphoglycerol; D003903:Deuterium; C028694:1-palmitoyl-2-oleoylphosphatidylcholine