title: Sertoli cell markers in the diagnosis of paediatric male hypogonadism.
abstract: During childhood, the pituitary-testicular axis is partially dormant: testosterone secretion decreases following a drop in luteinising hormone levels; follicle-stimulating hormone (FSH) levels also go down. Conversely, Sertoli cells are most active, as revealed by the circulating levels of anti-Müllerian hormone (AMH) and inhibin B. Therefore, hypogonadism can best be evidenced, without stimulation tests, if Sertoli cell function is assessed. Serum AMH is high from fetal life until mid-puberty. Testicular AMH production increases in response to FSH and is potently inhibited by androgens. Inhibin B is high in the first years of life, then decreases partially while remaining clearly higher than in females, and increases again at puberty. Serum AMH and inhibin B are undetectable in anorchid patients. In primary or central hypogonadism affecting the whole gonad established in fetal life or childhood, all testicular markers are low. Conversely, when hypogonadism only affects Leydig cells, serum AMH and inhibin B are normal. In males of pubertal age with central hypogonadism, AMH and inhibin B are low. Treatment with FSH provokes an increase in serum levels of both Sertoli cell markers, whereas human chorionic gonadotrophin (hCG) administration increases testosterone levels. In conclusion, measurement of serum AMH and inhibin B is helpful in assessing testicular function, without need for stimulation tests, and orientates the aetiological diagnosis of paediatric male hypogonadism.
authors.affiliation: Centro de Investigaciones Endocrinológicas (CEDIE, CONICET), Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina.
authors.forename: Romina P
authors.initials: RP
authors.lastname: Grinspon
authors.forename: Nazareth
authors.initials: N
authors.lastname: Loreti
authors.forename: Débora
authors.initials: D
authors.lastname: Braslavsky
authors.forename: Patricia
authors.initials: P
authors.lastname: Bedecarrás
authors.forename: Verónica
authors.initials: V
authors.lastname: Ambao
authors.forename: Silvia
authors.initials: S
authors.lastname: Gottlieb
authors.forename: Ignacio
authors.initials: I
authors.lastname: Bergadá
authors.forename: Stella M
authors.initials: SM
authors.lastname: Campo
authors.forename: Rodolfo A
authors.initials: RA
authors.lastname: Rey
mesh_terms: D054304:Anti-Mullerian Hormone / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002648:Child; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007006:Hypogonadism / Q000097:blood / Q000175:diagnosis*; D007265:Inhibins / Q000097:blood*; D008297:Male; D012708:Sertoli Cells / Q000378:metabolism*; D013737:Testis / Q000502:physiology
chemical_list: D015415:Biomarkers; C422364:inhibin B; D007265:Inhibins; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone