title: Reduced graphene oxide/PAMAM-silver nanoparticles nanocomposite modified electrode for direct electrochemistry of glucose oxidase and glucose sensing.
abstract: Reduced graphene oxide/PAMAM-silver nanoparticles nanocomposite (RGO-PAMAM-Ag) was synthesized by self-assembly of carboxyl-terminated PAMAM dendrimer (PAMAM-G3.5) on graphene oxide (GO) as growing template, and in-situ reduction of both AgNO(3) and GO under microwave irradiation. The RGO-PAMAM-Ag nanocomposite was used as a novel immobilization matrix for glucose oxidase (GOD) and exhibited excellent direct electron transfer properties for GOD with the rate constant (K(s)) of 8.59 s(-1). The fabricated glucose biosensor based on GOD electrode modified with RGO-PAMAM-Ag nanocomposite displayed satisfactory analytical performance including high sensitivity (75.72 μA mM(-1) cm(-2)), low detection limit (4.5 μM), an acceptable linear range from 0.032 mM to 1.89 mM, and also preventing the interference of some interfering species usually coexisting with glucose in human blood at the work potential of -0.25 V. These results indicated that RGO-PAMAM-Ag nanocomposite is a promising candidate material for high-performance glucose biosensors.
authors.affiliation: Laboratory of Advanced Materials, Fudan University, Shanghai 200433, PR China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Luo
authors.forename: Lihui
authors.initials: L
authors.lastname: Yuwen
authors.forename: Yujie
authors.initials: Y
authors.lastname: Han
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.forename: Xingrong
authors.initials: X
authors.lastname: Zhu
authors.forename: Lixing
authors.initials: L
authors.lastname: Weng
authors.forename: Lianhui
authors.initials: L
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D050091:Dendrimers / Q000737:chemistry; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry*; D012834:Silver / Q000737:chemistry
chemical_list: D050091:Dendrimers; D004800:Enzymes, Immobilized; C522260:PAMAM G3.5 dendrimer; D012834:Silver; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose