title: NF-κB p50 subunit knockout impairs late LTP and alters long term memory in the mouse hippocampus.
abstract: BACKGROUND
  Nuclear factor kappa B (NF-κB) is a transcription factor typically expressed with two specific subunits (p50, p65). Investigators have reported that NF-κB is activated during the induction of in vitro long term potentiation (LTP), a paradigm of synaptic plasticity and correlate of memory, suggesting that NF-κB may be necessary for some aspects of memory encoding. Furthermore, NF-κB has been implicated as a potential requirement in behavioral tests of memory. Unfortunately, very little work has been done to explore the effects of deleting specific NF-κB subunits on memory. Studies have shown that NF-κB p50 subunit deletion (p50-/-) leads to memory deficits, however some recent studies suggest the contrary where p50-/- mice show enhanced memory in the Morris water maze (MWM). To more critically explore the role of the NF-κB p50 subunit in synaptic plasticity and memory, we assessed long term spatial memory in vivo using the MWM, and synaptic plasticity in vitro utilizing high frequency stimuli capable of eliciting LTP in slices from the hippocampus of NF-κB p50-/- versus their controls (p50+/+).
  
  
  RESULTS
  We found that the lack of the NF-κB p50 subunit led to significant decreases in late LTP and in selective but significant alterations in MWM tests (i.e., some improvements during acquisition, but deficits during retention).
  
  
  CONCLUSIONS
  These results support the hypothesis that the NF-κ p50 subunit is required in long term spatial memory in the hippocampus.
authors.affiliation: Div'n, of Neurodegenerative Disorders, St, Boniface Hospital Research, Winnipeg, MB, Canada.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Oikawa
authors.forename: Gary L
authors.initials: GL
authors.lastname: Odero
authors.forename: Eric
authors.initials: E
authors.lastname: Platt
authors.forename: Melanie
authors.initials: M
authors.lastname: Neuendorff
authors.forename: Avril
authors.initials: A
authors.lastname: Hatherell
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bernstein
authors.forename: Benedict C
authors.initials: BC
authors.lastname: Albensi
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D017774:Long-Term Potentiation / Q000235:genetics* / Q000502:physiology; D018782:Maze Learning / Q000502:physiology; D057567:Memory, Long-Term / Q000502:physiology*; D051379:Mice; D057507:Mice, 129 Strain; D018345:Mice, Knockout; D052002:NF-kappa B p50 Subunit / Q000172:deficiency / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*
chemical_list: D052002:NF-kappa B p50 Subunit