title: 3D+t morphological processing: applications to embryogenesis image analysis.
abstract: We propose to directly process 3D + t image sequences with mathematical morphology operators using a new classification of the 3D+t structuring elements. Several methods (filtering, tracking, segmentation) dedicated to the analysis of 3D + t datasets of zebrafish embryogenesis are introduced and validated through a synthetic dataset. Then, we illustrate the application of these methods to the analysis of datasets of zebrafish early development acquired with various microscopy techniques. This processing paradigm produces spatio-temporal coherent results as it benefits from the intrinsic redundancy of the temporal dimension and minimizes the needs for human intervention in semi-automatic algorithms.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Luengo-Oroz
authors.forename: David
authors.initials: D
authors.lastname: Pastor-Escuredo
authors.forename: Carlos
authors.initials: C
authors.lastname: Castro-Gonzalez
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Faure
authors.forename: Thierry
authors.initials: T
authors.lastname: Savy
authors.forename: Benoit
authors.initials: B
authors.lastname: Lombardot
authors.forename: Jose L
authors.initials: JL
authors.lastname: Rubio-Guivernau
authors.forename: Louise
authors.initials: L
authors.lastname: Duloquin
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Ledesma-Carbayo
authors.forename: Paul
authors.initials: P
authors.lastname: Bourgine
authors.forename: Nadine
authors.initials: N
authors.lastname: Peyrieras
authors.forename: Andres
authors.initials: A
authors.lastname: Santos
mesh_terms: D000465:Algorithms; D000818:Animals; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000196:embryology*; D047108:Embryonic Development / Q000502:physiology*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D062211:Spatio-Temporal Analysis; D015027:Zebrafish / Q000033:anatomy & histology* / Q000196:embryology*