title: HOXA10 expression is decreased by testosterone in luteinized granulosa             cells in vitro.
abstract: Polycystic ovary syndrome (PCOS) is perhaps the most prevalent endocrine             disorder in women of reproductive age, characterized by elevated levels of circulating             androgens or clinical manifestations of androgen excess. The specific cytokine             profile of PCOS patients is probably related to the lower implantation rate, since             follicular fluid appears to function as an embryotrophic agent. For a better understanding             of the local regulation of human follicles, the present study investigated the             protein expression levels and cellular localization of HOXA10 in granulosa cells             (GCs) from women with normal ovarian function undergoing IVF due to their husbands             suffering from azoospermia. We demonstrated by immunohistochemical studies that             the expression of HOXA10 was mainly localized in the cytoplasm of GCs. Our data             indicate that these alterations were associated with changes in the expression             of ovarian transcription factors of HOXA10. GC dose-responsive decreases in HOXA10             protein were observed in response to physiological or supraphysiological concentrations             (10-4 to 10-7 M) of testosterone. These data reveal that testosterone may be involved             in HOXA10 gene regulation in GCs. Decreased HOXA10 expression in GCs treated with             testosterone suggest that this androgen is responsible for the decreased expression             of HOXA10 in PCOS patients.
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: He
authors.forename: Tian
authors.initials: T
authors.lastname: Li
authors.forename: Delong
authors.initials: D
authors.lastname: Yin
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: Qionghua
authors.initials: Q
authors.lastname: Chen
authors.forename: Ji
authors.initials: J
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Zhong
authors.forename: Demin
authors.initials: D
authors.lastname: Pu
mesh_terms: D000328:Adult; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism*; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011085:Polycystic Ovary Syndrome / Q000235:genetics / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D011962:Receptors, FSH / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D011962:Receptors, FSH; C069634:HOXA10 protein, human; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol