title: Indocyanine green-loaded biodegradable tumor targeting nanoprobes for in vitro and in vivo imaging.
abstract: Indocyanine green (ICG) is a near-infrared (NIR) ﬂuorescence dye for extensive biological application, but limited by its poor aqueous stability in vitro, concentration-dependent aggregation, rapid elimination from the body, and lack of target specificity. In this paper, to overcome these limitations, folate receptor-targeted, ICG dye-doped poly(d,l-lactide-co-glycolide) (PLGA) lipid nanoparticles (FA-ICG-PLGA-lipid NPs) were constructed by a single-step self-assemble and nanoprecipitation method. The prepared FA-ICG-PLGA-lipid NPs exhibited good biocompatibility, monodispersity, excellent NIR penetration ability, significant stability against photobleaching and long circulation time. The intracellular uptake experiment proved the targeting efficacy of the FA-ICG-PLGA-lipid NPs was more effective in folate receptor over-expressing MCF-7 cells than folate receptor negative A549 cells. Furthermore, the in vivo experiments showed the FA-ICG-PLGA-lipid NPs were specifically targeted to the tumor, and its circulation time was much longer than free ICG. These biocompatible and biodegradable NIR-NPs prove a potential application in tumor diagnosis and targeted imaging due to its high aqueous stability, excellent NIR optical properties and significantly targeting property in vivo.
authors.affiliation: CAS Key Lab of Health Informatics, Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
authors.forename: Cuifang
authors.initials: C
authors.lastname: Zheng
authors.forename: Mingbin
authors.initials: M
authors.lastname: Zheng
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Dongxue
authors.initials: D
authors.lastname: Jia
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhang
authors.forename: Bihua
authors.initials: B
authors.lastname: Shi
authors.forename: Zonghai
authors.initials: Z
authors.lastname: Sheng
authors.forename: Yifan
authors.initials: Y
authors.lastname: Ma
authors.forename: Lintao
authors.initials: L
authors.lastname: Cai
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000138:chemical synthesis; D005260:Female; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry
chemical_list: D003287:Contrast Media; D053769:Nanocapsules; D007208:Indocyanine Green