title: A highly sensitive, multiplex immunoassay using gold nanoparticle-enhanced signal amplification.
abstract: We describe a highly sensitive, multiplex immunoassay utilizing gold nanoparticles for conjugation of detection antibodies with signal generators. The method has been compared with conventional methods and evaluated for simultaneous detection of two different biomarkers in human serum.
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Ki-Cheol
authors.initials: KC
authors.lastname: Han
authors.forename: Eun Gyeong
authors.initials: EG
authors.lastname: Yang
authors.forename: Dae-Ro
authors.initials: DR
authors.lastname: Ahn
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015415:Biomarkers / Q000097:blood; D004247:DNA / Q000737:chemistry; D050556:Fatty Acid-Binding Proteins / Q000097:blood; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D000906:Antibodies; D015415:Biomarkers; D050556:Fatty Acid-Binding Proteins; D000509:alpha-Fetoproteins; D006046:Gold; D004247:DNA