title: Expression and role of nestin in human cervical intraepithelial neoplasia             and cervical cancer.
abstract: Nestin expression reportedly correlates with aggressive growth, metastasis,             poor prognosis and presence of cancer stem cells (CSCs) in various tumors. In             this study, we determined the expression and role of nestin in cervical intraepithelial             neoplasia (CIN) and cervical cancer. We performed immunohistochemical and in situ             hybridization analyses of nestin in 26 cases for each stage of CIN and 55 cervical             cancer tissue samples. To examine the role of nestin in cervical cancer cells,             we stably transfected expression vectors containing nestin cDNA into ME-180 cells.             We studied the effects of increased nestin expression on cell proliferation, cell             motility, invasion as well as sphere and soft agar formation. Nestin was not localized             in the squamous epithelium in normal cervical tissues, but it was weakly expressed             in the basal squamous epithelium of CIN 1. In CIN 2, nestin was localized to the             basal to lower 2/3 of the squamous epithelium, whereas in CIN 3, it was localized             to the majority of the squamous epithelium. Nestin was detected in all cases of             invasive cervical cancer. Nestin mRNA was expressed in both ME-180 and CaSki cells.             Growth rate, cell motility and invasion ability of stably nestin-transfected ME-180             cells were not different from empty vector-transfected ME-180 (mock cells). However,             the nestin-transfected ME-180 cells formed more colonies and spheres compared             to the mock cells. These findings suggest that nestin plays important roles in             carcinogenesis and tumor formation of cervical cancer cells. Nestin may closely             correlate with regulation of CSCs.
authors.affiliation: Department of Pathology and Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan.
authors.forename: Atsuki
authors.initials: A
authors.lastname: Sato
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ishiwata
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Hirobumi
authors.initials: H
authors.lastname: Asakura
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takeshita
authors.forename: Zenya
authors.initials: Z
authors.lastname: Naito
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D064231:Nestin; D018874:Spheroids, Cellular / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000473:pathology
chemical_list: D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin