title: Genetic modulation in Be-78 and Y Trypanosoma cruzi strains after long-term infection in Beagle dogs revealed by molecular markers.
abstract: The genetic profile of Trypanosoma cruzi was evaluated in parasite populations isolated from Beagle dogs experimentally infected with Be-78 and Y strains that present distinct biological and genetic characteristics. Molecular characterization of the isolates obtained 30days and 2years after infection was carried out. For typing MLEE, sequence polymorphisms of the mitochondrial cytochrome oxidase subunit II gene (COII) and RAPD profiles were used. The profiles of MLEE were the same for the parental Be-78 strains as their respective isolates. However, changes of MLEE profile were observed in two T. cruzi isolates from dogs inoculated with Y strain. Changes in the mitochondrial DNA (COII) and RAPD profiles of the Y strain were also observed. The dendogram constructed by UPGMA with RAPD results indicated two major branches. Global data show that the genetic modulation in polyclonal strains during the long-term infection occurred and was strain-dependent. This study still suggests that each host (here each dog) harbors a determinate T. cruzi population that may change or be modulated throughout long-term infection. This might to hinder the observation of correlation between the genetics of T. cruzi and their biological properties and behavior in different host species due to the complexity of the parasite-host interaction in which probably the genetic background of both should be considered.
authors.affiliation: Departamento de Farmácia, Escola de Farmácia, Universidade Federal de Ouro Preto, Rua Costa Sena 171, Centro, CEP 35400-000 Ouro Preto, MG, Brazil. vanjaveloso@ef.ufop.br
authors.forename: Vanja Maria
authors.initials: VM
authors.lastname: Veloso
authors.forename: Paulo Marcos da Matta
authors.initials: PM
authors.lastname: Guedes
authors.forename: Marta
authors.initials: M
authors.lastname: de Lana
authors.forename: Helen Rodrigues
authors.initials: HR
authors.lastname: Martins
authors.forename: Cláudia Martins
authors.initials: CM
authors.lastname: Carneiro
authors.forename: Antônia Cláudia Jácome
authors.initials: AC
authors.lastname: da Câmara
authors.forename: Daniella Alchaar
authors.initials: DA
authors.lastname: D'Ávila
authors.forename: Ivo Santana
authors.initials: IS
authors.lastname: Caldas
authors.forename: Lúcia Maria da Cunha
authors.initials: LM
authors.lastname: Galvão
authors.forename: Egler
authors.initials: E
authors.lastname: Chiari
authors.forename: Maria Terezinha
authors.initials: MT
authors.lastname: Bahia
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D016000:Cluster Analysis; D004285:Dogs; D003576:Electron Transport Complex IV / Q000235:genetics; D004586:Electrophoresis; D005260:Female; D050259:Genes, Mitochondrial / Q000235:genetics; D005819:Genetic Markers; D006790:Host-Parasite Interactions; D008297:Male; D015800:Protozoan Proteins / Q000235:genetics; D014349:Trypanosoma cruzi / Q000145:classification / Q000201:enzymology / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D015800:Protozoan Proteins; D003576:Electron Transport Complex IV