title: Molecular detection and incidence of human papillomavirus in neonates: methodology and a pilot study in a medical center.
abstract: OBJECTIVE
  Human papillomavirus (HPV) infection can cause laryngeal papillomas in children. Vertical transmission has been confirmed. This study aimed to establish a sensitive molecular diagnostic method and understand the incidence of the HPV-6 and HPV-11 in neonates with intubation.
  
  
  METHODS
  We enrolled 108 newborns between October 2007 and January 2010. All neonates were intubated due to underlying disease. The specimens were collected via endotracheal aspiration. DNA of HPV types 6 and 11 was detected by real-time PCR and nested PCR.
  
  
  RESULTS
  HPV-DNA was detected in eight of the 108 newborns studied. Seven respiratory specimens tested positive for HPV-11 and one was positive for HPV-6. The HPV 6/11 detection rate in neonates was 7.4% (8/108).
  
  
  CONCLUSIONS
  A rapid, sensitive, specific, and reproducible RT-PCR method and nest PCR were developed for the detection and differentiation of HPV-6 and HPV-11 genomic variants in a single PCR reaction. The assays are of great value for clinical and epidemiologic studies of HPV-6 and HPV-11 infections. Neonatal HPV colonization may be related to juvenile-onset recurrent respiratory papillomatosis. The transmission route may be from mother to child. The clinical significance of neonatal carriage of HPV-6 or HPV-11 warrants further study.
authors.affiliation: Department of Pediatrics, National Taiwan University Hospital, College of Medicine, Taipei, Taiwan.
authors.forename: Chun-Fu
authors.initials: CF
authors.lastname: Tai
authors.forename: Tsung-Pei
authors.initials: TP
authors.lastname: Tsou
authors.forename: Wu-Shiun
authors.initials: WS
authors.lastname: Hsieh
authors.forename: Chien-Yi
authors.initials: CY
authors.lastname: Chen
authors.forename: Hung-Chieh
authors.initials: HC
authors.lastname: Chou
authors.forename: Po-Nien
authors.initials: PN
authors.lastname: Tsao
authors.forename: Chien-Hui
authors.initials: CH
authors.lastname: Hsu
authors.forename: Yi-Jen
authors.initials: YJ
authors.lastname: Liau
authors.forename: Chun-Yi
authors.initials: CY
authors.lastname: Lu
authors.forename: Pei-Lan
authors.initials: PL
authors.lastname: Shao
authors.forename: Luan-Yin
authors.initials: LY
authors.lastname: Chang
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Huang
mesh_terms: D000046:Academic Medical Centers; D001483:Base Sequence; D015331:Cohort Studies; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D052140:Human papillomavirus 11 / Q000235:genetics / Q000302:isolation & purification*; D052163:Human papillomavirus 6 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D015994:Incidence; D007231:Infant, Newborn; D007442:Intubation, Intratracheal / Q000706:statistics & numerical data; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D030361:Papillomavirus Infections / Q000821:virology*; D010865:Pilot Projects; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D016415:Sequence Alignment
chemical_list: D004279:DNA, Viral