title: Mechanisms of inhibition of elemene on human lens epithelial cell proliferation in vitro.
abstract: OBJECTIVE
  To study the effects of elemene (Ele) on proliferation and cell cycle of human lens epithelial cells B3 (HLE-B3) and the mechanisms of its signal transduction.
  
  
  METHODS
  Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 80mg/L Ele for 24 hours. The inhibitory effects of Ele on the proliferation of HLE-B3 cells were evaluated by MTT method. The effect of Ele on HLE-B3 cell cycle was analyzed by flow cytometry(FCM). The expressions of protein kinase A (PKA) and protein kinase G (PKG) of HLE-B3 were also analyzed by FCM.
  
  
  RESULTS
  Ele altered the cell cycle of HLE-B3 and effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. Ele up-regulated PKA and down-regulated the expression of PKG in HLE-B3 cell.
  
  
  CONCLUSIONS
  Ele inhibits HLE-B3 proliferation, making it an attractive potential agent in regimens to treat after-cataracts.
authors.affiliation: Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian College of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Hu
authors.forename: Xiu-Rong
authors.initials: XR
authors.lastname: Huang
authors.forename: Ming-Xin
authors.initials: MX
authors.lastname: Qi
authors.forename: Bu-Yuan
authors.initials: BY
authors.lastname: Hou