title: A novel label-free electrochemical aptasensor based on graphene-polyaniline composite film for dopamine determination.
abstract: A novel label-free electrochemical aptasensor based on graphene-polyaniline (GR-PANI) nanocomposites film for dopamine (DA) determination was reported. The resulting GR-PANI layer exhibited good current response for DA determination. The good electron transfer activity might be attributed to the effect of GR and PANI. The highly conductive and biocompatible nanostructure of GR-PANI nanocomposites was characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). To quantify the amount of DA, the peaks of square-wave voltammetry (SWV) were monitored using the redox couple of an [Fe(CN)(6)](4-/3-) probe. The electrochemical aptasensor showed a linear response to DA in the range 0.007-90 nmol/L and a limit of detection of 0.00198 nmol/L (S/N=3). The electrochemical aptasensor was successfully tested on human serum samples.
authors.affiliation: College of Resources and Environment, University of Jinan, Jinan 250022, PR China.
authors.forename: Su
authors.initials: S
authors.lastname: Liu
authors.forename: Xianrong
authors.initials: X
authors.lastname: Xing
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.forename: Wenjing
authors.initials: W
authors.lastname: Lian
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Cui
authors.forename: Jiadong
authors.initials: J
authors.lastname: Huang
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D058266:Dielectric Spectroscopy; D004298:Dopamine / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D052156:SELEX Aptamer Technique
chemical_list: D000814:Aniline Compounds; D052157:Aptamers, Nucleotide; C416807:polyaniline; D006108:Graphite; D004298:Dopamine