title: Rapidly serum-degradable hydrogel templating fabrication of spherical tissues and curved tubular structures.
abstract: Development of the techniques for fabricating three-dimensional tissues still poses significant challenges for tissue engineering. We used hydrogels obtained from phenol-substituted amylopectin (AP-Ph) as templates for preparing multicellular spherical tissues (MSTs) and endothelialized curved tubular structures in type I collagen gel. AP-Ph hydrogel microparticles of diameter 200 µm and fibers of diameter 500 µm disappeared within hours of soaking in a serum-containing medium. HeLa cells and human endothelial cells were enclosed in the microparticles and hydrogel fibers, respectively, and then embedded in Ca-alginate microcapsules or the collagen gel. The enclosed cells were released in cavities formed by hydrogel degradation in the serum-containing medium. The released HeLa cells in the spherical cavities grew and formed MSTs, eventually filling the cavities. The spherical tissues were easily harvested by liquefying the Ca-alginate hydrogel microcapsule membrane by chelation using sodium citrate. The released endothelial cells grew on the tubular cavity surfaces and formed tubular structures. An endothelial cell network was formed by cell migration into the collagen gel. These results demonstrate the potential of serum-degradable AP-Ph hydrogels in constructing three-dimensional tissues.
authors.affiliation: Division of Chemical Engineering, Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka, Japan. sakai@cheng.es.osaka-u.ac.jp
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.forename: Hitomi
authors.initials: H
authors.lastname: Inagaki
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Matsuyama
authors.forename: Takanori
authors.initials: T
authors.lastname: Kihara
authors.forename: Jun
authors.initials: J
authors.lastname: Miyake
authors.forename: Koei
authors.initials: K
authors.lastname: Kawakami
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
mesh_terms: D020341:Absorbable Implants; D000464:Alginates / Q000378:metabolism; D000687:Amylopectin / Q000378:metabolism; D002478:Cells, Cultured; D002951:Citrates / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D042783:Endothelial Cells; D004847:Epithelial Cells; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000378:metabolism*; D044967:Serum / Q000378:metabolism*; D000077559:Sodium Citrate; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000464:Alginates; D002951:Citrates; D006603:Hexuronic Acids; D000077559:Sodium Citrate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid; D003094:Collagen; D000687:Amylopectin