title: The biological behaviors of rat dermal fibroblasts can be inhibited by high levels of MMP9.
abstract: OBJECTIVE
  To explore the effects of the high expression of MMP9 on biological behaviors of fibroblasts.
  
  
  METHODS
  High glucose and hyperhomocysteine were used to induce MMP9 expression in skin fibroblasts. Cell proliferation was detected by flow cytometry and cell viability by CCK-8. ELISA assay was used to detect collagen (hydroxyproline) secretion. Scratch test was employed to evaluate horizontal migration of cells and transwell method to evaluate vertical migration of cells.
  
  
  RESULTS
  The mRNA and protein expressions of MMP9 and its protease activity were significantly higher in cells treated with high glucose and hyperhomocysteine than those in control group. At the same time, the S-phase cell ratio, proliferation index, cell viability, collagen (hydroxyproline) secretion, horizontal migration rate, and the number of vertical migration cells decreased in high-glucose and hyperhomocysteine-treated group. Tissue inhibitor of metalloproteinase 1 (TIMP1), which inhibits the activity of MMP9, recovered the above biological behaviors.
  
  
  CONCLUSIONS
  High expression of MMP9 in skin fibroblasts could be induced by cultureing in high glucose and hyperhomocysteine medium, which inhibited cell biological behaviors. Inhibitions could be reversed by TIMP1. The findings suggested that MMP9 deters the healing of diabetic foot ulcers by inhibiting the biological behaviors of fibroblasts.
authors.affiliation: Department of Endocrinology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang West Road, Guangzhou 510120, China.
authors.forename: Sheng-Neng
authors.initials: SN
authors.lastname: Xue
authors.forename: Juan
authors.initials: J
authors.lastname: Lei
authors.forename: Chuan
authors.initials: C
authors.lastname: Yang
authors.forename: Diao-Zhu
authors.initials: DZ
authors.lastname: Lin
authors.forename: Li
authors.initials: L
authors.lastname: Yan
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D006710:Homocysteine / Q000494:pharmacology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051381:Rats; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D006710:Homocysteine; D020780:Matrix Metalloproteinase 9; D005947:Glucose