title: Paracrine effects of adipose-derived stem cells on keratinocytes and dermal fibroblasts.
abstract: BACKGROUND
  Adipose-derived stem cells (ASCs) are mesenchymal stem cells that have recently been applied to tissue repair and regeneration. Keratinocytes and dermal fibroblasts play key roles in cutaneous wound healing.
  
  
  OBJECTIVE
  We investigated the paracrine effects of ASCs on HaCaT cells (i.e., immortalized human keratinocytes) and human dermal fibroblasts to explore the mechanism of the effects of ASCs on cutaneous wound healing.
  
  
  METHODS
  HaCaT cells and primary cultured human dermal fibroblasts were treated with 50% conditioned medium of ASCs (ASC-CM). Viability, in vitro wound healing, and fibroblast-populated collagen lattice contraction assays were conducted, and reverse transcription-polymerase chain reaction (RT-PCR) for the type I procollagen α1 chain gene was performed.
  
  
  RESULTS
  The proliferation of HaCaT cells and fibroblasts was increased by ASC-CM in the viability assay. ASC-CM promoted in vitro wound healing of HaCaT cells and increased the contraction of the fibroblast-populated collagen lattice. RT-PCR showed that the transcription of the type I procollagen α1 chain gene in fibroblasts was upregulated by ASC-CM.
  
  
  CONCLUSIONS
  The stimulatory effect of ASC on cutaneous wound healing may be partially mediated by paracrine effects of ASCs on other skin cells. Application of ASCs or ASC-derived molecules could be an innovative therapeutic approach in the treatment of chronic wounds and other conditions.
authors.affiliation: Department of Dermatology, Dongguk University Ilsan Hospital, Goyang, Korea.
authors.forename: Seung Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Sang Yun
authors.initials: SY
authors.lastname: Jin
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Song
authors.forename: Kyle K
authors.initials: KK
authors.lastname: Seo
authors.forename: Kwang Hyun
authors.initials: KH
authors.lastname: Cho