title: Construction of adiponectin-encoding plasmid DNA and overexpression in mice in vivo.
abstract: The effects of elevated adiponectin (ADN) plasma levels on food intake, body weight, and lipid deposition of wild-type mice through ADN gene transfer using hydrodynamic based-gene delivery (HD) were investigated. The administration of pTarget/ADN significantly increased the blood ADN levels on days 1, 3, and 7 as well as food intake and body weight. Reverse transcription polymerase chain reaction (RT-PCR) was used to investigate the key-function genes involved in lipid deposition on epididymal fat, gastrocnemius, and extensor digitorum longus on days 1 and 7. The amounts of adipose triglyceride lipase, hormone-sensitive lipase, and lipoprotein lipase mRNA in the three samples significantly increased. We determined sirtuin 1 (SIRT1), forkhead box O3 (FOXO3a), and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) gene expression and protein level in these samples. The amounts of SIRT1, FOXO3a, and PGC-1α mRNA in epididymal fat, gastrocnemius, and extensor digitorum longus remarkably increased. However, a significant increase in SIRT1 and PGC-1α protein levels was only observed in extensor digitorum longus. These results suggest that high doses of ADN can increase food intake and body weight. Elevated ADN levels may also affect fat deposition on the adipose tissue and skeletal muscles of wild-type mice via SIRT1, FOXO3a, and its downstream targets, including PGC-1α.
authors.affiliation: Institute of Feed Science, Zhejiang University, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Yu Hang Tang Road 866, Hangzhou 310058, China.
authors.forename: Yan-Na
authors.initials: YN
authors.lastname: Huang
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Qi
authors.forename: Lan
authors.initials: L
authors.lastname: Xiang
authors.forename: Yi-Zhen
authors.initials: YZ
authors.lastname: Wang
mesh_terms: D000091162:AMP-Activated Protein Kinase Kinases; D052242:Adiponectin / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism; D052436:Adipose Tissue, White / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics; D004247:DNA / Q000235:genetics*; D004435:Eating / Q000235:genetics; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D018014:Gene Transfer Techniques; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D008055:Lipids / Q000235:genetics; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010957:Plasmids / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism; D002787:Sterol Esterase / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors
chemical_list: D052242:Adiponectin; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; C474033:FoxO3 protein, mouse; D008055:Lipids; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; D015534:Trans-Activators; D014157:Transcription Factors; C475419:adiponectin receptor 1, mouse; C475421:adiponectin receptor 2, mouse; D004247:DNA; D011494:Protein Kinases; D000091162:AMP-Activated Protein Kinase Kinases; D002787:Sterol Esterase; D008049:Lipase; D008071:Lipoprotein Lipase; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1