title: Identification of a Toll-like receptor 1 in guinea fowl (Agelastes niger).
abstract: Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns, thus playing important roles in host defense. This study determined the first sequence of a TLR1 type 1 in the guinea fowl (GFTLR1). The open reading frame of GFTLR1 type 1 contains 2,115 nucleotides and encodes 705 amino acids. Amino acid analysis indicated that GFTLR1 type 1 shares 92.3 % homology with the green jungle fowl, 92.1 % with the chicken, 90.4 % with the turkey, and 84.4 % with Cooper's hawk. Genetic patterns were identified within the TLR1 type 1 of the chicken and the guinea fowl. GFTLR1 type 1 was found to have 92 polymorphic amino acid sites, of which 16 were in the leucine-rich repeat (LRR) domain, 3 in a C-terminal LRR domain, and 6 in a Toll/interleukin-1 receptor domain. The data showed that avian TLR1 type 1 genes are under purifying selection and highly conserved, because dN/dS was less than 1.
authors.affiliation: Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China. wuyanhua@ccmu.edu.cn
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Wu
authors.forename: Wenke
authors.initials: W
authors.lastname: Ruan
authors.forename: Defeng
authors.initials: D
authors.lastname: Cui
authors.forename: Huanrong
authors.initials: H
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D046094:Galliformes / Q000235:genetics*; D000090263:Leucine-Rich Repeat Proteins; D016366:Open Reading Frames; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics; D017472:Receptors, Interleukin-1 / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017385:Sequence Homology; D013045:Species Specificity; D051194:Toll-Like Receptor 1 / Q000235:genetics*
chemical_list: D030161:Avian Proteins; D000090263:Leucine-Rich Repeat Proteins; D011506:Proteins; D017472:Receptors, Interleukin-1; D051194:Toll-Like Receptor 1