title: Simple strategy of repeated batch cultivation for enhanced production of 1,3-propanediol using Clostridium diolis.
abstract: Repeated batch cultivation (empty-and-fill protocol) using obligate anaerobe Clostridium diolis was attempted in the present study to improve the production of 1,3-propanediol (1,3-PD). In repeated batch operation, 20 % (v/v) culture broth was removed from the bioreactor and supplemented with an equal volume of fresh nutrient medium when the residual glycerol concentration in the bioreactor decreased below 15 g/L. Four cycles of culture broth withdrawal and subsequent replacement resulted in achieving a 1,3-PD concentration of 67.8 g/L with a productivity of 1.04 g/L/h at the end of 65 h. This represented a 1,3-PD concentration and productivity enhancement by 2.6-fold and 1.5-fold, respectively, as compared to batch 1,3-PD fermentation. This is the first report on the use of repeated batch mode of bioreactor operation for enhanced 1,3-PD production.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Guneet
authors.initials: G
authors.lastname: Kaur
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Srivastava
authors.forename: Subhash
authors.initials: S
authors.lastname: Chand
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D003013:Clostridium / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D005285:Fermentation; D007700:Kinetics; D011409:Propylene Glycols / Q000378:metabolism*
chemical_list: D011409:Propylene Glycols; C041787:1,3-propanediol