title: Effect of the umbilical cord blood acid-base status and gas values on the yield of mononuclear cells and CD34⁺ cells.
abstract: OBJECTIVE
  To investigate the influence of umbilical cord blood (CB) acid-base status and gas values on the yield of mononuclear cells and CD34⁺ cells, pH, pCO₂, pO₂, HCO₃⁻ and base excess were measured in arterial CB samples obtained from normal full-term deliveries. The relationship of these values with the yield of mononuclear cells and CD34⁺ cells detected in venous CB was analyzed.
  
  
  METHODS
  A total of 145 CB units were collected from full-term vaginal deliveries at a single hospital. Immediately after delivery, a segment of the umbilical cord was double clamped, and arterial CB was analyzed to determine the acid-base status and gases. Venous CB was collected in a sterile collection bag and processed for cell separation within 24 h of collection. The relationship between umbilical arterial acid-base status, each gas value, and the yield of mononuclear cells and CD34⁺ cells was analyzed.
  
  
  RESULTS
  Statistically significant correlations were observed between the net weight of CB and the total mononuclear and CD34⁺ cell counts. In addition, there was a negative correlation between the mononuclear cell counts and pH, but a positive correlation between the mononuclear cell counts and pCO₂. However, no significant differences were observed between the primipara and multipara groups in terms of the net weight of CB, total mononuclear cell counts and total CD34⁺ cell counts.
  
  
  CONCLUSIONS
  The findings of the present study show that the mononuclear cell counts are correlated with arterial CB pH and pCO₂, suggesting the involvement of fetal hypoxia on the yield of mononuclear cells.
authors.affiliation: Department of Disability and Health, Hirosaki University Graduate School of Health Sciences, Hirosaki, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Ebina
authors.forename: Atsuko
authors.initials: A
authors.lastname: Omori
authors.forename: Ayako
authors.initials: A
authors.lastname: Tarakida
authors.forename: Tomoka
authors.initials: T
authors.lastname: Ogasawara
authors.forename: Mami
authors.initials: M
authors.lastname: Manabe
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Katagiri
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kashiwakura
mesh_terms: D000136:Acid-Base Equilibrium; D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D001784:Blood Gas Analysis; D001800:Blood Specimen Collection / Q000379:methods*; D002469:Cell Separation; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D005311:Fetal Hypoxia / Q000097:blood / Q000276:immunology; D019337:Hematologic Neoplasms / Q000628:therapy; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007564:Japan; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000637:transplantation; D011247:Pregnancy; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D018952:Antigens, CD34