title: Depletion of mitochondrial DNA stabilizes C1qTNF-related protein 6 mRNA in muscle cells.
abstract: Mutation and reduction of mitochondrial DNA (mtDNA) have been suggested as factors in the pathogenesis of several metabolic diseases. Recently, we demonstrated that C1qTNF-related protein-6 (CTRP6) is involved in fatty acid metabolism in muscle cells. In this study, we showed that expression of CTRP6 was up-regulated in mtDNA-depleted C2C12 cells, which displayed a marked decrease in cellular mtDNA and ATP content. Replacement of mtDNA normalized the expression level of CTRP6 similar to that in normal C2C12 cells, indicating that CTRP6 expression was up-regulated by mtDNA depletion. However, CTRP6 promoter activity remained unchanged in mtDNA-depleted cells. We also found that mtDNA depletion inhibited decay of CTRP6 mRNA. Taken together, mtDNA depletion induces an increase in CTRP6 expression by increasing mRNA stability.
authors.affiliation: Department of Biochemistry, Dongguk University School of Medicine, Gyeongju, Korea.
authors.forename: Mi-Jin
authors.initials: MJ
authors.lastname: Kim
authors.forename: Wan
authors.initials: W
authors.lastname: Lee
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Park
authors.forename: Seung-Yoon
authors.initials: SY
authors.lastname: Park
mesh_terms: D052242:Adiponectin / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D004272:DNA, Mitochondrial / Q000378:metabolism*; D051379:Mice; D011401:Promoter Regions, Genetic; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D052242:Adiponectin; C543616:C1QTNF8 protein, human; D004272:DNA, Mitochondrial; D012333:RNA, Messenger