title: A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China.
abstract: An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level of Shiga toxin 2 in Xuzhou21 induced by mitomycin C was 68.6 times of that under non-inducing conditions, twice of that induced in Sakai (32.7 times) and 15 times higher than that induced in EDL933 (4.5 times). Our study shows that ST96 is a novel clone and provided significant new insights into the evolution of virulence of E. coli O157:H7.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Changping, Beijing, China.
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Xiong
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Ruiting
authors.initials: R
authors.lastname: Lan
authors.forename: Changyun
authors.initials: C
authors.lastname: Ye
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Huaiqi
authors.initials: H
authors.lastname: Jing
authors.forename: Yiting
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhemin
authors.initials: Z
authors.lastname: Zhou
authors.forename: Xuemei
authors.initials: X
authors.lastname: Bai
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Cui
authors.forename: Xia
authors.initials: X
authors.lastname: Luo
authors.forename: Ailan
authors.initials: A
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Shaomin
authors.initials: S
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D002681:China / Q000453:epidemiology; D016207:Cytokines / Q000378:metabolism; D004196:Disease Outbreaks / Q000706:statistics & numerical data*; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D005843:Geography; D006463:Hemolytic-Uremic Syndrome / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D022641:Shiga Toxin 2 / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D016207:Cytokines; D022641:Shiga Toxin 2