title: A thiol-mediated active membrane transport of selenium by erythroid anion exchanger 1 protein.
abstract: In this paper, we describe a thiol-mediated and energy-dependent membrane transport of selenium by erythroid anion exchanger 1 (AE1, also known as band 3 protein). The AE1 is the most abundant integral protein of red cell membranes and plays a critical role in the carbon dioxide transport system in which carbon dioxide is carried as bicarbonate in the plasma. This protein mediates the membrane transport of selenium, an essential antioxidant micronutrient, from red cells to the plasma in a manner that is distinct from the already known anion exchange mechanism. In this pathway, selenium bound to the cysteine 93 of the hemoglobin β chain (Hb-Cysβ93) is transported by the relay mechanism to the Cys317 of the amino-terminal cytoplasmic domain of the AE1 on the basis of the intrinsic interaction between the two proteins and is subsequently exported to the plasma via the Cys843 of the membrane-spanning domain. The selenium export did not occur in plain isotonic buffer solutions and required thiols, such as albumin, in the outer medium. Such a membrane transport mechanism would also participate in the export pathways of the nitric oxide vasodilator activity and other thiol-reactive substances bound to the Hb-Cysβ93 from red cells to the plasma and/or peripherals.
authors.affiliation: Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Hongoh
authors.forename: Mamoru
authors.initials: M
authors.lastname: Haratake
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fuchigami
authors.forename: Morio
authors.initials: M
authors.lastname: Nakayama
mesh_terms: D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry / Q000378:metabolism*; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D010949:Plasma / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012643:Selenium / Q000378:metabolism*; D018036:Selenium Compounds / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D018036:Selenium Compounds; D013438:Sulfhydryl Compounds; D013440:Sulfides; C059755:selenotrisulfide; D012643:Selenium