title: L-leucine 5-hydroxylase of Nostoc punctiforme is a novel type of Fe(II)/α-ketoglutarate-dependent dioxygenase that is useful as a biocatalyst.
abstract: L-Leucine 5-hydroxylase (LdoA) previously found in Nostoc punctiforme PCC 73102 is a novel type of Fe(II)/α-ketoglutarate-dependent dioxygenase. LdoA catalyzed regio- and stereoselective hydroxylation of L-leucine and L-norleucine into (2S,4S)-5-hydroxyleucine and (2S)-5-hydroxynorleucine, respectively. Moreover, LdoA catalyzed sulfoxidation of L-methionine and L-ethionine in the same manner as previously described L-isoleucine 4-hydroxylase. Therefore LdoA should be a promising biocatalyst for effective production of industrially useful amino acids.
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hibi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawashima
authors.forename: Pavel M
authors.initials: PM
authors.lastname: Sokolov
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Smirnov
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kodera
authors.forename: Masakazu
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
authors.forename: Kenzo
authors.initials: K
authors.lastname: Yokozeki
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
mesh_terms: D049308:Dioxygenases / Q000302:isolation & purification* / Q000378:metabolism*; D005001:Ethionine / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000378:metabolism*; D007930:Leucine / Q000031:analogs & derivatives* / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D009646:Norleucine / Q000378:metabolism; D046937:Nostoc / Q000201:enzymology*; D012451:Safrole / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D007656:Ketoglutaric Acids; D009646:Norleucine; D008715:Methionine; D007501:Iron; D049308:Dioxygenases; D007930:Leucine; D012451:Safrole; C005746:sulfoxide; D005001:Ethionine