title: Fluid dynamics modeling for synchronizing surface plasmon resonance and quartz crystal microbalance as tools for biomolecular and targeted drug delivery studies.
abstract: We have used computational fluid dynamics modeling (CFD) to synchronize the flow conditions in the flow channels of two complementary surface-sensitive characterization techniques: surface plasmon resonance (SPR) and quartz crystal microbalance (QCM). Since the footprint of the flow channels of the two devices is specified by their function, the flow behavior can only be varied either by altering the height of the flow channel, or altering the volumetric rate of flow (flow rate) through the channel. The relevant quantity that must be calibrated is the shear strain on the measurement surface (center and bottom) of the flow channel. Our CFD modeling shows that the flow behavior is in the Stokes flow regime. We were thus able to generate a scaling expression with parameters for flow rate and flow channel height for each of the two devices: f(QCM)=2.64f(SPR)(h(QCM)/h(SPR)(2), where f(QCM) and f(SPR) are the flow rates in the SPR and QCM flow channels, respectively, and h(QCM)/h(SPR) is the ratio of the heights of the two channels. We demonstrate the success of our calibration procedure through the combined use of commercially available SPR and QCM flow channel devices on both a biomolecular interaction system of surface immobilized biotin and streptavidin and a targeted drug delivery model system of biotinylated liposomes interacting with a streptavidin functionalized surface.
authors.affiliation: Division of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Helsinki, Finland.
authors.forename: Tapani
authors.initials: T
authors.lastname: Viitala
authors.forename: Huamin
authors.initials: H
authors.lastname: Liang
authors.forename: Mayur
authors.initials: M
authors.lastname: Gupta
authors.forename: Thomas
authors.initials: T
authors.lastname: Zwinger
authors.forename: Marjo
authors.initials: M
authors.lastname: Yliperttula
authors.forename: Alex
authors.initials: A
authors.lastname: Bunker
mesh_terms: D001710:Biotin / Q000737:chemistry; D002138:Calibration; D016503:Drug Delivery Systems / Q000379:methods* / Q000592:standards; D008081:Liposomes; D008956:Models, Chemical; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods / Q000592:standards*; D012212:Rheology; D033081:Shear Strength; D019809:Streptavidin / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods* / Q000592:standards*
chemical_list: D008081:Liposomes; D001710:Biotin; D019809:Streptavidin