title: Mycoplasma hominis in Cuban Trichomonas vaginalis isolates: association with parasite genetic polymorphism.
abstract: Trichomonas vaginalis can be naturally infected with intracellular Mycoplasma hominis. This bacterial infection may have implications for trichomonal virulence and disease pathogenesis. The objective of the study was to report the presence of M. hominis in Cuban T. vaginalis isolates and to describe the association between the phenotype M. hominis infected with RAPD genetic polymorphism of T. vaginalis. The Random Amplified Polymorphic DNA (RAPD) technique was used to determine genetic differences among 40 isolates of T. vaginalis using a panel of 30 random primers and these genetic data were correlated with the infection of isolates with M. hominis. The trees drawn based on RAPD data showed no relations with metronidazole susceptibility and significantly association with the presence of M. hominis (P=0.043), which demonstrates the existence of concordance between the genetic relatedness and the presence of M. hominis in T. vaginalis isolates. This result could point to a predisposition of T. vaginalis for the bacterial enters and/or survival.
authors.affiliation: Departamento de Parasitología, Instituto de Medicina Tropical Pedro Kourí, Autopista Novia del Mediodía km 6½, Marianao 13, La Habana, Cuba. fraga@ipk.sld.cu
authors.forename: Jorge
authors.initials: J
authors.lastname: Fraga
authors.forename: Nadia
authors.initials: N
authors.lastname: Rodríguez
authors.forename: Carmen
authors.initials: C
authors.lastname: Fernández
authors.forename: Brian
authors.initials: B
authors.lastname: Mondeja
authors.forename: Idalia
authors.initials: I
authors.lastname: Sariego
authors.forename: Aymé
authors.initials: A
authors.lastname: Fernández-Calienes
authors.forename: Lazara
authors.initials: L
authors.lastname: Rojas
mesh_terms: D003462:Cuba; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D005260:Female; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D019535:Mycoplasma hominis / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D008973:Tenericutes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D014246:Trichomonas vaginalis / Q000235:genetics* / Q000382:microbiology*; D014566:Urogenital System / Q000382:microbiology / Q000469:parasitology
chemical_list: D004269:DNA, Bacterial