title: The stability and activity of respiratory Complex II is cardiolipin-dependent.
abstract: Respiratory Complex II of the mitochondrial inner membrane serves as a link between the tricarboxylic acid cycle and the electron transport chain. Complex II dysfunction has been implicated in a wide range of heritable mitochondrial diseases, including cancer, by a mechanism that likely involves the production of reactive oxygen species (ROS). Using Complex II enzymes reconstituted into nanoscale lipid bilayers (nanodiscs) with varying lipid composition, we demonstrate for the first time that the phospholipid environment, specifically the presence of cardiolipin, is critical for the assembly and enzymatic activity of the complex, as well as in the curtailment of ROS production.
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA. christine.schwall@uconn.edu
authors.forename: Christine T
authors.initials: CT
authors.lastname: Schwall
authors.forename: Victoria L
authors.initials: VL
authors.lastname: Greenwood
authors.forename: Nathan N
authors.initials: NN
authors.lastname: Alder
mesh_terms: D002308:Cardiolipins / Q000502:physiology*; D042963:Electron Transport Complex II / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D008928:Mitochondria / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D002308:Cardiolipins; D010715:Phosphatidylglycerols; D017382:Reactive Oxygen Species; C478189:respiratory complex II; D042963:Electron Transport Complex II