title: Proteomic analysis of the pinworm Syphacia muris (Nematoda: Oxyuridae), a parasite of laboratory rats.
abstract: Syphacia muris (Nematoda: Oxyuridae) is a ubiquitous nematode that commonly infects rats in the laboratory which can interfere in the development of biological assays. The somatic extract of S. muris adults collected from infected rats was investigated using a proteomic approach. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 359 proteins were accurately identified from the worms. The largest protein families consisted of metabolic enzymes and those involved in the nucleic metabolism and cell cycle. Proteins of transmembrane receptors and those involved in protein metabolism, chaperones, structural and motor, signalling and calcium-binding proteins also were identified in the proteome of S. muris. Proteome array of S. muris may contribute to further elucidation of biological system of S. muris as well as host-parasite relationships.
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Sotillo
authors.forename: María
authors.initials: M
authors.lastname: Trelis
authors.forename: Alba
authors.initials: A
authors.lastname: Cortés
authors.forename: María Luz
authors.initials: ML
authors.lastname: Valero
authors.forename: Manuel Sánchez
authors.initials: MS
authors.lastname: del Pino
authors.forename: José
authors.initials: J
authors.lastname: Guillermo Esteban
authors.forename: Antonio
authors.initials: A
authors.lastname: Marcilla
authors.forename: Rafael
authors.initials: R
authors.lastname: Toledo
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism; D007756:Laboratory Animal Science; D009349:Nematode Infections / Q000469:parasitology / Q000662:veterinary*; D010124:Oxyuroidea / Q000235:genetics* / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D012376:Rodent Diseases / Q000469:parasitology*; D059467:Transcriptome
chemical_list: D015801:Helminth Proteins