title: Cultivable bacteria populations associated with leaves of banana and plantain plants and their antagonistic activity against Mycosphaerella fijiensis.
abstract: Mycosphaerella fijiensis is the etiological agent of Black Sigatoka, a fungal disease that affects production of banana and plantain crops in tropical regions. The sizes of cultivable epiphytic and endophytic bacterial populations, aerobic endospore forming bacteria (AEFB), and antagonist bacteria against M. fijiensis isolated from three Musa spp. cultivars from Urabá (Colombia) were studied, in order to find a suitable screening strategy to isolate antagonistic bacteria. Most of the variability found in the epiphytic and endophytic bacterial community sizes among fruit trees was explained by the cultivar differences. We found population sizes ranging from 1.25 × 10(3) to 9.64 × 10(5) CFU/g of fresh leaf and found that 44 % of total cultivable bacteria belong to the AEFB group. We isolated 648 AEFB from three different cultivars and assessed their antagonistic activity against M. fijiensis using the cell-free supernatant obtained from bacterial liquid cultures in three different in vitro assays. Five percent of those bacteria showed higher percent inhibition than the positive control Bacillus subtilis UA321 has (percent inhibition = 84 ± 5) in the screening phase. Therefore, they were selected as antagonistic bacteria against the pathogen. The strains with the highest percentage of antagonism were found in older leaves for the three cultivars, given support to recommend this group of leaves for future samplings. Some of these isolated bacteria affected the mycelium and ascospores morphology of the fungus. They also presented in vitro characteristics related to a successful colonization of the phylloplane such as indolic compounds, surfactant production, and biofilm formation, which makes them possible, potential candidates as biological control agents.
authors.affiliation: Grupo GIPAB. Departamento de Ingeniería de Procesos, Universidad EAFIT, Carrera 49 No. 7 Sur-50, Medellín, Colombia.
authors.forename: Isabel
authors.initials: I
authors.lastname: Ceballos
authors.forename: Sandra
authors.initials: S
authors.lastname: Mosquera
authors.forename: Mónica
authors.initials: M
authors.lastname: Angulo
authors.forename: John J
authors.initials: JJ
authors.lastname: Mira
authors.forename: Luz Edith
authors.initials: LE
authors.lastname: Argel
authors.forename: Daniel
authors.initials: D
authors.lastname: Uribe-Velez
authors.forename: Magally
authors.initials: M
authors.lastname: Romero-Tabarez
authors.forename: Sergio
authors.initials: S
authors.lastname: Orduz-Peralta
authors.forename: Valeska
authors.initials: V
authors.lastname: Villegas
mesh_terms: D000898:Antibiosis; D001203:Ascomycota / Q000254:growth & development / Q000472:pathogenicity*; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D028521:Musa / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology*; D010943:Plantago / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial / Q000254:growth & development
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S