title: 6-Methoxyluteolin from Chrysanthemum zawadskii var. latilobum suppresses histamine release and calcium influx via down-regulation of FcεRI α chain expression.
abstract: Mast cells and basophils are important effector cells in immunoglobulin-E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of 6-methoxyluteolin, isolated from Chrysanthemum zawadskii, in the FcεRI-mediated allergic reaction. We determined that 6-methoxyluteolin inhibited anti-FcεRI α chain antibody (CRA-1)-induced histamine release, as well as elevation of intracellular calcium concentration [Ca2+]i in a dose-dependent manner. Moreover, the inhibitory effects of 6-methoxyluteolin on the cell surface expression and the mRNA level of the FcεRI α chain were determined by flow cytometric analysis and reverse transcription-polymerase chain reaction (RTPCR), respectively. Therefore, these results show that 6- methoxyluteolin is a potent inhibitor of histamine release and calcium influx via down-regulation of the FcεRI α chain.
authors.affiliation: Institute of Marine Life Science, Pukyong National University, Busan, 608-737, Korea.
authors.forename: Sun-Yup
authors.initials: SY
authors.lastname: Shim
authors.forename: Jeong-Ro
authors.initials: JR
authors.lastname: Park
authors.forename: Dae-Seok
authors.initials: DS
authors.lastname: Byun
mesh_terms: D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002118:Calcium / Q000276:immunology*; D002460:Cell Line; D031202:Chrysanthemum / Q000737:chemistry*; D015536:Down-Regulation / Q000187:drug effects*; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy / Q000235:genetics / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D047311:Luteolin / Q000302:isolation & purification / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D017455:Receptors, IgE / Q000235:genetics* / Q000276:immunology
chemical_list: D018926:Anti-Allergic Agents; D010936:Plant Extracts; D017455:Receptors, IgE; D007073:Immunoglobulin E; D047311:Luteolin; D002118:Calcium