title: Dual effects of silibinin treatment on autophagy-regulated dermal apoptosis retardation and epidermal apoptosis up-regulation in UVB-induced skin inflammation.
abstract: Skin inflammation induced by ultraviolet B (UVB) radiation is characterized by migration and chemotaxis of inflammatory cells, epidermic thickening and erythema. Apoptosis and autophagy of epidermal and dermal cells are involved in its development through the adjustment of balance between cell survival and death. In this study, the role of balance between cell survival and apoptosis in dermis and epidermis in UVB-induced skin inflammation and the effect of autophagy on the balance were elucidated, and the protective mechanism of silibinin was investigated through the examination of the influence of autophagy activation or inhibition on erythema, migration, and chemotaxis of inflammatory cells as well as apoptosis adjustments. In UVB-irradiated controls, dermal apoptosis was retarded and the survival of inflammatory cells was promoted through the up-regulation of dermal autophagic level; epidermal apoptosis was increased through the down-regulation of epidermal autophagic level, causing migration and chemotaxis of neutrophils and mast cells as well as skin erythema. In silibinin-treated group (50 mg/kg/day for 4 days), dermal apoptosis was increased through inhibiting dermal autophagy; improper adjustment of epidermal apoptosis was attenuated through promoting epidermal autophagy, presenting dual effects on the balance between autophagy and apoptosis of epidermal and dermal cells and the protection.
authors.affiliation: China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang, 110016, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.forename: Yuanchao
authors.initials: Y
authors.lastname: Ye
authors.forename: Weiwei
authors.initials: W
authors.lastname: Liu
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Tashiro
authors.forename: Satoshi
authors.initials: S
authors.lastname: Onodera
authors.forename: Fengying
authors.initials: F
authors.lastname: Gu
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Wang
authors.forename: Takashi
authors.initials: T
authors.lastname: Ikejima
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy; D015153:Blotting, Western; D020405:Dermis / Q000187:drug effects; D004817:Epidermis / Q000187:drug effects; D005260:Female; D008297:Male; D008407:Mast Cells / Q000187:drug effects; D051379:Mice; D020944:Silybum marianum / Q000737:chemistry; D015394:Molecular Structure; D012639:Seeds / Q000737:chemistry; D000077385:Silybin; D012838:Silymarin / Q000737:chemistry / Q000494:pharmacology*; D012867:Skin / Q000276:immunology / Q000473:pathology / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D012838:Silymarin; D000077385:Silybin