title: Comparison of the Fc glycosylation of fetal and maternal immunoglobulin G.
abstract: Human immunoglobulin G (IgG) molecules are composed of two Fab portions and one Fc portion. The glycans attached to the Fc portions of IgG are known to modulate its biological activity as they influence interaction with both complement and various cellular Fc receptors. IgG glycosylation changes significantly with pregnancy, showing a vast increase in galactosylation and sialylation and a concomitant decrease in the incidence of bisecting GlcNAc. Maternal IgGs are actively transported to the fetus by the neonatal Fc receptor (FcRn) expressed in syncytiotrophoblasts in the placenta, providing the fetus and newborn with immunological protection. Two earlier reports described significant differences in total glycosylation between fetal and maternal IgG, suggesting a possible glycosylation-selective transport via the placenta. These results might suggest an alternative maternal transport pathway, since FcRn binding to IgG does not depend on Fc-glycosylation. These early studies were performed by releasing N-glycans from total IgG. Here, we chose for an alternative approach analyzing IgG Fc glycosylation at the glycopeptide level in an Fc-specific manner, providing glycosylation profiles for IgG1 and IgG4 as well as combined Fc glycosylation profiles of IgG2 and 3. The analysis of ten pairs of fetal and maternal IgG samples revealed largely comparable Fc glycosylation for all the analyzed subclasses. Average levels of galactosylation, sialylation, bisecting GlcNAc and fucosylation were very similar for the fetal and maternal IgGs. Our data suggest that the placental IgG transport is not Fc glycosylation selective.
authors.affiliation: Department of Experimental Immunohematology, Sanquin Research, and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Helga K
authors.initials: HK
authors.lastname: Einarsdottir
authors.forename: Maurice H J
authors.initials: MH
authors.lastname: Selman
authors.forename: Rick
authors.initials: R
authors.lastname: Kapur
authors.forename: Sicco
authors.initials: S
authors.lastname: Scherjon
authors.forename: Carolien A M
authors.initials: CA
authors.lastname: Koeleman
authors.forename: André M
authors.initials: AM
authors.lastname: Deelder
authors.forename: C Ellen
authors.initials: CE
authors.lastname: van der Schoot
authors.forename: Gestur
authors.initials: G
authors.lastname: Vidarsson
authors.forename: Manfred
authors.initials: M
authors.lastname: Wuhrer
mesh_terms: D002853:Chromatography, Liquid; D005260:Female; D006031:Glycosylation; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D007231:Infant, Newborn; D013058:Mass Spectrometry; D011247:Pregnancy; D011961:Receptors, Fc / Q000737:chemistry / Q000378:metabolism*
chemical_list: D007074:Immunoglobulin G; D011961:Receptors, Fc