title: Specificity, sensitivity, and operability of RSID™-urine for forensic identification of urine: comparison with ELISA for Tamm-Horsfall protein.
abstract: In this study, the specificity, sensitivity, and operability of RSID™-Urine, a new immunochromatographic test for urine identification, was evaluated and compared with ELISA detection of Tamm-Horsfall protein (THP). Urine was successfully identified among other body fluids using RSID™-Urine and ELISA detection of THP. The detection limit of RSID™-Urine equated to 0.5 μL of urine; although the sensitivity of RSID™-Urine may be lower than that of ELISA detection of THP, it is thought to be sufficient for application to casework samples. However, results from RSID™-Urine must be interpreted with caution when the sample may have been contaminated with blood or vaginal fluid, because this might inhibit urine detection. The RSID™-Urine assay can be performed in just 15 min by dropping the extracted sample onto the test cassette. Therefore, RSID™-Urine should be an effective tool for the forensic identification of urine, in addition to ELISA detection of THP.
authors.affiliation: Third Biology Section, First Department of Forensic Science, National Research Institute of Police Science, Kashiwa, Chiba, Japan. tomoko@nrips.go.jp
authors.forename: Tomoko
authors.initials: T
authors.lastname: Akutsu
authors.forename: Ken
authors.initials: K
authors.lastname: Watanabe
authors.forename: Koichi
authors.initials: K
authors.lastname: Sakurada
mesh_terms: D000906:Antibodies / Q000032:analysis; D001774:Blood Chemical Analysis; D002846:Chromatography, Affinity / Q000191:economics; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D012463:Saliva / Q000737:chemistry; D012661:Semen / Q000737:chemistry; D012680:Sensitivity and Specificity; D013542:Sweat / Q000737:chemistry; D058949:Uromodulin / Q000276:immunology / Q000652:urine*; D014621:Vagina / Q000737:chemistry
chemical_list: D000906:Antibodies; C548612:UMOD protein, human; D058949:Uromodulin