title: Utility of an oxidation reaction for the spectrophotometric determination of acarbose in controlled release tablets at various simulated gastrointestinal media.
abstract: A sensitive kinetic method for spectrophotometric determination of acarbose is developed and validated for the determination of the drug in bulk and pharmaceutical formulations. The drug was estimated in simulated gastrointestinal media i.e., 0.1 M HCl (pH 1.2) and phosphate buffer (pH 6.8). The method involves the oxidation of acarbose by treating it with a strong oxidizing agent (potassium permanganate (1 x 10(-2) M)) in alkaline media. The reaction kinetics was determined for 20 min at room temperature. The reaction followed first order kinetics and the absorbance of the corresponding manganate ions produced was determined at 610 nm. The absorbance-concentration plot was found to be rectilinear over the concentration range of 2-20 microg/mL. The proposed method was used for estimation of the drug in a novel controlled release dosage form. Thus, the method developed was simple, reproducible and can be successfully applied for the determination of the drug in simulated gastrointestinal fluid.
authors.affiliation: University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, 160014, India. sinha_vr@rediffmail.com
authors.forename: Vivek Ranjan
authors.initials: VR
authors.lastname: Sinha
authors.forename: Ruchita V
authors.initials: RV
authors.lastname: Kumar
mesh_terms: D020909:Acarbose / Q000032:analysis*; D002021:Buffers; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D003692:Delayed-Action Preparations; D004339:Drug Compounding; D004791:Enzyme Inhibitors / Q000032:analysis*; D005750:Gastric Juice / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007419:Intestinal Secretions / Q000737:chemistry*; D007700:Kinetics; D010084:Oxidation-Reduction; D011196:Potassium Permanganate / Q000737:chemistry; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000737:chemistry; D013053:Spectrophotometry / Q000592:standards; D013607:Tablets; D013678:Technology, Pharmaceutical / Q000379:methods* / Q000592:standards
chemical_list: D002021:Buffers; D003692:Delayed-Action Preparations; D004791:Enzyme Inhibitors; D013607:Tablets; D011196:Potassium Permanganate; D012972:Sodium Hydroxide; D006851:Hydrochloric Acid; D020909:Acarbose