title: Virus scaffolds as enzyme nano-carriers.
abstract: The cooperative organization of enzymes by cells is a key feature for the efficiency of living systems. In the field of nanotechnologies, effort currently aims at mimicking this natural organization. Nanoscale resolution and high-registration alignment are necessary to control enzyme distribution in nano-containers or on the surface of solid supports. Virus capsid self-assembly is driven by precise supramolecular combinations of protein monomers, which have made them attractive building blocks to engineer enzyme nano-carriers (ENCs). We discuss some examples of what in our opinion constitute the latest advances in the use of plant viruses, bacteriophages and virus-like particles (VLPs) as nano-scaffolds for enzyme selection, enzyme confinement and patterning, phage therapy, raw material processing, and single molecule enzyme kinetics studies.
authors.affiliation: UMR 1332 Biologie du Fruit et Pathologie, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France.
authors.forename: Daniela
authors.initials: D
authors.lastname: Cardinale
authors.forename: Noëlle
authors.initials: N
authors.lastname: Carette
authors.forename: Thierry
authors.initials: T
authors.lastname: Michon
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002213:Capsid / Q000737:chemistry; D003001:Cloning, Molecular; D004798:Enzymes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D049329:Nanostructures; D036103:Nanotechnology / Q000379:methods*; D010942:Plant Viruses / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D004798:Enzymes