title: [Effect of Budesonide on   Smad4,  PDGF-A  and PAI-1 in a rat model of pulmonary fibrosis].
abstract: OBJECTIVE
  To observe the effect of Budesonide (BUD) on TGF-β1,  PDGF-A,  Smad4 and PAI-1 in lung tissue in pulmonary fibrosis rats.
  
  
  METHODS
  Forty-five  adult female Wistar rats were randomly divided  into normal solution (NS) group,  BUD group  and bleomycin (BLM)  group. 9 g/L NaCl solution was instilled into the tratracheaes in NS group,  and BLM were used in BUD group and BLM group. NS group and BLM group were inhaled with 9 g/L NaCl solution once everyday at day 0-6,  and BUD group were used with BUD. Five  rats in every group were killed at 7th, 14th 28th day. The appearances of alveolitis and fibrosis were displayed in HE and Masson staining. The expressions of TGF-β1,  PDGF-A,  Smad4 and PAI-1 in lung tissue were detected by immunohistochemistry.
  
  
  RESULTS
  The extent of  the alveolitis in   BUD group at 7th, 14th day were significantly lower than  that in  BLM group(P<0.05).  The fibrosis and the expressions of TGF-β1,  PDGF-A,  Smad4 and PAI-1 in lung tissues  at 7th, 14th,  28th day,  BUD group were significantly lower than BLM group(P<0.05).
  
  
  CONCLUSIONS
  After inhaled BUD,  the expressions of TGF-β1,  PDGF-A,  Smad4 and PAI-1 in lung tissue could be decreased,  and the extent of alveolitis and pulmonary fibrosis could be improved in bleomycin-induced pulmonary fibrosis rats.
authors.affiliation: Department of Respiratory, the Affliliated Hospital of Lu Zhou Medical College, Luzhou, China.
authors.forename: Ju-ping
authors.initials: JP
authors.lastname: Chen
authors.forename: Xian-ming
authors.initials: XM
authors.lastname: Fan
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Zhan
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D019819:Budesonide / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis*; D010982:Platelet-Derived Growth Factor / Q000032:analysis*; D011658:Pulmonary Fibrosis / Q000188:drug therapy* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051901:Smad4 Protein / Q000032:analysis*; D053773:Transforming Growth Factor beta1 / Q000032:analysis
chemical_list: D000893:Anti-Inflammatory Agents; D017395:Plasminogen Activator Inhibitor 1; D010982:Platelet-Derived Growth Factor; D051901:Smad4 Protein; C490811:Smad4 protein, rat; D053773:Transforming Growth Factor beta1; C075278:platelet-derived growth factor A; D019819:Budesonide