title: Anti-PRRSV effect and mechanism of sodium tanshinone IIA sulfonate in vitro.
abstract: This experiment was conducted to study the antiviral activities of sodium tanshinone IIA sulfonate (STS) against porcine reproductive and respiratory syndrome virus (PRRSV) and its mechanism. Anti-PRRSV activities of STS were observed on Marc-145 cells by using visualization of cytopathologic effect assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, and its antiviral mechanism was determined by time-of-addition assay, adsorption inhibition assay, and virucidal assay. The results showed that STS could reduce the damage of PRRSV to Marc-145 cells, with the inhibition ratio exceeding to 100%, at the maximum non-cytotoxic concentration. The time-of-addition and virucidal assays indicated that the anti-PRRSV activities of STS could be due to inhibiting the virus replication or/and inactivating the virus directly. The inhibition of the virus attachment was not discovered in adsorption inhibition assay. The results proved that STS had strong anti-PRRSV activity and encouraged for further exploration of STS.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Xi-Yun
authors.initials: XY
authors.lastname: Bai
authors.forename: Li
authors.initials: L
authors.lastname: Niu
authors.forename: Mei-Qin
authors.initials: MQ
authors.lastname: Song
authors.forename: Yao-Gui
authors.initials: YG
authors.lastname: Sun
authors.forename: Jun-Bing
authors.initials: JB
authors.lastname: Jiang
authors.forename: Hong-Quan
authors.initials: HQ
authors.lastname: Li
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D010616:Phenanthrenes / Q000737:chemistry / Q000494:pharmacology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects*; D012964:Sodium / Q000494:pharmacology; D013552:Swine
chemical_list: D000998:Antiviral Agents; D010616:Phenanthrenes; C024894:tanshinone II A sodium sulfonate; D012964:Sodium