title: Gene polymorphism of interleukin-1 alpha and beta in keratocystic odontogenic tumors.
abstract: OBJECTIVE
  Odontogenic keratocysts have a different growth mechanism and biologic behavior in comparison with more common dentigerous and radicular cysts. It was reclassified as keratocystic odontogenic tumor (KCOT). The proliferative activity of the epithelial cells of KCOT has a close relationship with tissue levels of interleukin-1 (IL-1). Moreover, IL-1 increases the expression of several matrix metalloproteinases in the fibroblasts of adjacent stroma and activates the osteoclastogenesis process. So it plays an important role in the activity, spread, and local aggressiveness of this tumor. Therefore, it seems that the gene polymorphism of the cytokines of the IL-1 family is influential in the pathogenesis of KCOT and the patients' susceptibility to disease.
  
  
  METHODS
  A total of 38 blood samples of patients suffering from KCOT and 150 blood samples of healthy patients were assessed using PCR-SSP. The blood samples were assessed for the following polymorphisms: interleukin-1 alpha (-889) and interleukin-1 beta (-511). Following up the patients, we found six recurrent and one syndromic cases.
  
  
  RESULTS
  By comparing the case and control groups, we observed the significant dominance of allele T over C, and genotype TT over CC and CT in IL-1α, although no significant difference was seen in the allele frequency and genotypes regarding IL-1β.
  
  
  CONCLUSIONS
  The function of IL-1α has a significant relationship with KCOT. Its effective genotype associated with pathogenesis, growth, local invasion, and recurrence is TT.
authors.affiliation: Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Eshghyar
authors.forename: Behrouz
authors.initials: B
authors.lastname: Nikbin
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Amirzargar
authors.forename: Ali
authors.initials: A
authors.lastname: Dehghani Nazhvani
authors.forename: Yadollah
authors.initials: Y
authors.lastname: Shakiba
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D003596:Cytosine; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006720:Homozygote; D006801:Humans; D053582:Interleukin-1alpha / Q000097:blood / Q000235:genetics*; D053583:Interleukin-1beta / Q000097:blood / Q000235:genetics*; D008297:Male; D008339:Mandibular Neoplasms / Q000097:blood / Q000235:genetics; D008441:Maxillary Neoplasms / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000473:pathology; D009808:Odontogenic Tumors / Q000097:blood / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D013577:Syndrome; D013941:Thymine; D055815:Young Adult
chemical_list: D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D003596:Cytosine; D013941:Thymine