title: McrA and 16S rRNA gene analysis suggests a novel lineage of Archaea phylogenetically affiliated with Thermoplasmatales in human subgingival plaque.
abstract: Based on the molecular analysis of human subgingival plaque samples from 30 periodontitis patients a novel lineage of Archaea within the phylogenetic radiation of Thermoplasmatales was identified in 10% of cases. Co-occurrence of unique 16S rRNA gene and mcrA gene sequences suggests that this lineage corresponds to a hitherto unknown group of methanogens.
authors.affiliation: Division of Oral Microbiology and Immunology, Department of Medical Microbiology, RWTH Aachen University Hospital, Germany. hhorz@ukaachen.de
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Horz
authors.forename: Ilse
authors.initials: I
authors.lastname: Seyfarth
authors.forename: Georg
authors.initials: G
authors.lastname: Conrads
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D001105:Archaea / Q000235:genetics / Q000302:isolation & purification; D019843:Archaeal Proteins / Q000235:genetics*; D017124:Conserved Sequence; D003773:Dental Plaque / Q000382:microbiology*; D019143:Evolution, Molecular; D005260:Female; D006801:Humans; D016013:Likelihood Functions; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D058889:Molecular Typing; D010518:Periodontitis / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D054749:Ribosome Subunits, Small, Archaeal / Q000235:genetics*; D017422:Sequence Analysis, DNA; D019603:Thermoplasmales / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D019843:Archaeal Proteins; D012336:RNA, Ribosomal, 16S