title: Central ventilatory and cardiovascular actions of calcitonin gene-related peptide in unanesthetized trout.
abstract: Calcitonin gene-related peptide (CGRP) and its receptors are widely distributed in the tissues of teleost fish, including the brain, but little is known about the ventilatory and cardiovascular effects of the peptide in these vertebrates. The present study was undertaken to compare the central and peripheral actions of graded doses (5-50 pmol) of trout CGRP on ventilatory and cardiovascular variables in unanesthetized rainbow trout. Compared with vehicle, intracerebroventricular injection of CGRP significantly elevated the ventilation frequency (f(V)) and the ventilation amplitude (V(AMP)) and, consequently, the total ventilation (V(TOT)). The maximum hyperventilatory effect of CGRP (V(TOT): +300%), observed at a dose of 50 pmol, was mostly due to its stimulatory action on V(AMP) (+200%) rather than f(V) (+30%). In addition, CGRP produced a significant and dose-dependent increase in mean dorsal aortic blood pressure (P(DA)) (50 pmol: +40%) but the increase in heart rate (f(H)) was not significant. Intra-arterial injections of CGRP were without effect on the ventilatory variables but significantly and dose-dependently elevated P(DA) (50 pmol: +36%) without changing f(H). At the highest dose tested, this hypertensive phase was preceded by a rapid and transient hypotensive response. In conclusion, our study suggests that endogenous CGRP within the brain of the trout may act as a potent neurotransmitter and/or neuromodulator in the regulation of cardio-ventilatory functions. In the periphery, endogenous CGRP may act as a local and/or circulating hormone preferentially involved in vasoregulatory mechanisms.
authors.affiliation: Université Européenne de Bretagne, Université de Brest, INSERM UMR101, Brest, CHU de Brest, France. jean-claude.lemevel@univ-brest.fr
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Le Mével
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lancien
authors.forename: Nagi
authors.initials: N
authors.lastname: Mimassi
authors.forename: Marc
authors.initials: M
authors.lastname: Kermorgant
authors.forename: J Michael
authors.initials: JM
authors.lastname: Conlon
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000187:drug effects / Q000502:physiology; D015740:Calcitonin Gene-Related Peptide / Q000008:administration & dosage / Q000494:pharmacology* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D007269:Injections, Intra-Arterial; D007276:Injections, Intraventricular; D008297:Male; D017686:Oncorhynchus mykiss / Q000502:physiology*; D012119:Respiration / Q000187:drug effects
chemical_list: D015740:Calcitonin Gene-Related Peptide