title: Assessment of microbial contamination of radiographic equipment and materials during intraoral imaging procedures.
abstract: OBJECTIVE
  Aim of the present study was to assess microbial contamination of radiology procedures.
  
  
  METHODS
  Patients who needed radiographic exams were selected and the bisecting technique was used: G1 - (control): absence of plastic barrier and overgloving or disinfectant solutions; G2 - alcohol spraying; G3 - protection of the film with a plastic barrier and alcohol spray; G4 - protection of film with plastic barrier, use of overgloving and alcohol spray. The following regions were assessed: trigger switch, X-ray tube, sleeve of the portable dark chamber, water, developer and fixer. The areas for microbiological sample collection were standardized with a label cut internally so that the hollow area was 5 cm long and 2 cm wide. One mL of the developer, water and fixer were also collected before and after developing the films. The samples were incubated under anaerobiosis and aerobiosis. The results were submitted to the Cochran's Q and Mann-Whitney tests.
  
  
  RESULTS
  The sleeve of the developing chamber showed greater anaerobic contamination followed by the X-ray tube and only the use of alcohol associated with mechanical barriers was efficient to control this microbiota. The trigger showed higher aerobic microbial contamination and the use of alcohol or alcohol associated with mechanical barriers was efficient to control this microbiota. The developing solutions presented no significant growth of anaerobic and aerobic bacteria.
  
  
  CONCLUSIONS
  The characteristic of an aerobic or anaerobic microbial strain influences microbial contamination while radiographic projections are being taken and the use of alcohol associated with a plastic barrier and overgloving is indicated to reduce this microbiota.
authors.affiliation: Restorative Dentistry, PUC-Campinas Dental School, Vinhedo, Campinas, São Paulo, Brazil. slpinho@puc-campinas.edu.br
authors.forename: S L
authors.initials: SL
authors.lastname: Pinheiro
authors.forename: S C
authors.initials: SC
authors.lastname: Martoni
authors.forename: R R
authors.initials: RR
authors.lastname: Ogera
mesh_terms: D001420:Bacteria, Aerobic / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D058491:Bacterial Load; D003764:Dental Materials; D004203:Disinfection; D004866:Equipment Contamination; D000431:Ethanol; D006801:Humans; D054892:Metagenome; D010969:Plastics; D011861:Radiography, Dental / Q000295:instrumentation*; D012996:Solutions; D014962:X-Ray Film
chemical_list: D003764:Dental Materials; D010969:Plastics; D012996:Solutions; D000431:Ethanol