title: Association between urinary 8-hydroxydeoxyguanosine, an indicator of oxidative stress, and the cardio-ankle vascular index in hypertensive patients.
abstract: OBJECTIVE
  Oxidative stress has been recently postulated to be an important factor in the pathogenesis and development of arteriosclerosis. Although urinary 8-hydroxydeoxyguanosine (8-OHdG) is clinically used as a marker of oxidative stress, its usefulness in diagnosing arteriosclerosis has not been fully examined. This study aimed to evaluate the association between urinary 8-OHdG and the cardioankle vascular index (CAVI) as a marker of arterial stiffness in hypertensive patients.
  
  
  METHODS
  We enrolled 100 hypertensive patients (70 ± 10 years) who had been taking antihypertensive medications for at least one year. Urinary 8-OHdG levels were measured by an immunochromatographic assay (ICR-001; Selista Inc., Tokyo, Japan). CAVIs were measured at the same visit.
  
  
  RESULTS
  Urinary 8-OHdG was correlated with smoking habits (r=0.382, p<0.001) and CAVIs (r= 0.223, p= 0.026). Multiple linear regression analysis revealed two independent determinants of urinary 8-OHdG: smoking habits (β=0.501, p<0.001) and CAVI (β=0.325, p=0.001). In addition, CAVIs were correlated with age (r= 0.600, p<0.001), BMI (r=-0.348, p<0.001), systolic blood pressure (r= 0.343, p<0.001), pulse pressure (r= 0.358, p<0.001), serum creatinine level (r=0.408, p<0.001), urinary 8-OHdG level (r= 0.223, p= 0.026), and diabetes (r= 0.210, p=0.036). Multiple linear regression analysis revealed two independent determinants of CAVI: age (β= 0.568, p<0.001) and 8-OHdG (β=0.357, p<0.001).
  
  
  CONCLUSIONS
  Elevated CAVI is independently associated with an elevated urinary 8-OHdG level in hypertensive patients.
authors.affiliation: Department of Integrated Medicine, Kagawa University, Kagawa, Japan. masugata@med.kagawa-u.ac.jp
authors.forename: Hisashi
authors.initials: H
authors.lastname: Masugata
authors.forename: Shoichi
authors.initials: S
authors.lastname: Senda
authors.forename: Koji
authors.initials: K
authors.lastname: Murao
authors.forename: Michio
authors.initials: M
authors.lastname: Inukai
authors.forename: Takashi
authors.initials: T
authors.lastname: Himoto
authors.forename: Naohisa
authors.initials: N
authors.lastname: Hosomi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Okada
authors.forename: Fuminori
authors.initials: F
authors.lastname: Goda
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D055109:Ankle Brachial Index; D000843:Ankle Joint / Q000098:blood supply* / Q000473:pathology; D015415:Biomarkers / Q000032:analysis*; D003430:Cross-Sectional Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D006973:Hypertension / Q000473:pathology / Q000652:urine*; D007564:Japan; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011379:Prognosis; D059289:Vascular Stiffness / Q000502:physiology*
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine