title: Comamonas guangdongensis sp. nov., isolated from subterranean forest sediment, and emended description of the genus Comamonas.
abstract: A facultatively anaerobic bacterium, strain CY01(T), isolated from subterranean forest sediment collected from Guangdong Province, China, was investigated using a polyphasic taxonomic approach. The cells were short rods, Gram-negative, non-sporulating and motile. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CY01(T) showed highest sequence similarities to Comamonas thiooxydans S23(T) (98.0 %), Comamonas testosteroni JCM 5832(T) (97.9 %), Comamonas koreensis KCTC 12005(T) (97.7 %) and Comamonas odontotermitis LMG 23579(T) (97.0 %). The major respiratory quinone was ubiquinone-8. The major cellular fatty acids were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0 and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). Based on the phylogenetic analysis, DNA-DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics, strain CY01(T) was clearly distinguishable from all recognized species of the genus Comamonas and should be classified as a representative of a novel species of the genus, for which the name Comamonas guangdongensis sp. nov. is proposed. The type strain is CY01(T) ( = CCTCC AB 2011133(T) = KACC 16241(T)).
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.|Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Shungui
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Chunyuan
authors.initials: C
authors.lastname: Wu
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Fangbai
authors.initials: F
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D020585:Comamonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8