title: Protective effect of celastrol in rat cerebral ischemia model: down-regulating p-JNK, p-c-Jun and NF-κB.
abstract: Oxidative stress and inflammatory damage play an important role in cerebral ischemic pathogenesis and may represent a target for treatment. Celastrol has been proved to elicit a vanity of biological effects through its anti-oxidant, anti-inflammatory properties in the treatment of Alzheimer's disease, systemic lupus erythematosus, and rheumatoid arthritis. However, little is known regarding the effect of celastrol in the acute phase of ischemic stroke. This study investigated the potential protective effects of celastrol and underlying mechanisms in cerebral ischemia. We used a permanent middle cerebral artery occlusion (pMCAO) model and administered celastrol intraperitoneally immediately after stroke. At 24h after stroke, we found that celastrol dramatically reduced neurological deficit, brain water content and infarct sizes, and downregulated the expression of p-JNK, p-c-Jun and NF-κB. The results indicated that celastrol may have the possibility of protective effect against ischemic injury, and this effect may be through downregulation of the expression of p-JNK, p-c-Jun and NF-κB.
authors.affiliation: Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, PR China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: He
authors.forename: Xiangjian
authors.initials: X
authors.lastname: Zhang
authors.forename: Zongjie
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhang
authors.forename: Lipeng
authors.initials: L
authors.lastname: Dong
authors.forename: Yinxue
authors.initials: Y
authors.lastname: Xing
authors.forename: Chaohui
authors.initials: C
authors.lastname: Wang
authors.forename: Huimin
authors.initials: H
authors.lastname: Qiao
authors.forename: Chunhua
authors.initials: C
authors.lastname: Zhu
authors.forename: Yulin
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation / Q000187:drug effects*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D053978:Pentacyclic Triterpenes; D010766:Phosphorylation / Q000187:drug effects; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014315:Triterpenes / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D016328:NF-kappa B; D018696:Neuroprotective Agents; D053978:Pentacyclic Triterpenes; D016755:Proto-Oncogene Proteins c-jun; D014315:Triterpenes; D048031:JNK Mitogen-Activated Protein Kinases; C050414:celastrol