title: In vitro selection of bacteriophage promoters employing a terminally capped template DNA and a streptavidin-binding aptamer.
abstract: In vitro selection system for the isolation of bacteriophage promoters was developed. Employing the system, one round of the selection achieved ∼20-30 fold enrichment of an active promoter from mixtures with an inactive one. In addition, active T7 promoter variants were successfully selected from a library with six randomized bases.
authors.affiliation: Department of Basic Medical Sciences, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. sohuchi@ims.u-tokyo.ac.jp
authors.forename: Shoji
authors.initials: S
authors.lastname: Ohuchi
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism; D017123:Bacteriophage T7 / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D019809:Streptavidin / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D004279:DNA, Viral; D009841:Oligonucleotides; D014764:Viral Proteins; D019809:Streptavidin; C070852:RNA polymerase SP6; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases