title: HPV 9G DNAChip: Based on the 9G DNAChip technology.
abstract: The novel HPV 9G DNAChips were developed for the detection and discrimination of the HPV genotypes in the clinical samples. The HPV 9G DNAChip established high SBR of 50-70 and 100% target-specific hybridization after 30min hybridization and 2×2min washing at 25°C. We compared the genotyping results of the 959 HPV positive and 82 HPV negative clinical samples by the HPV 9G DNAChip and the sequencing; the results are in 100% agreement. The HPV 9G DNAChip efficiently discriminate 19 HPV genotypes in the 959 HPV positive clinical samples. The results of HPV 9G DNAChip were 100% identical with the sequencing analysis in the detection and discrimination of HPV genotypes in the HPV negative clinical samples. The high SBR, 100% target-specific hybridization, and 25°C hybridization and washing makes the HPV 9G DNAChip a promising diagnostic tool for the accurate HPV genotyping.
authors.affiliation: Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Republic of Korea.
authors.forename: Keum-Soo
authors.initials: KS
authors.lastname: Song
authors.forename: Satish Balasaheb
authors.initials: SB
authors.lastname: Nimse
authors.forename: Heejung
authors.initials: H
authors.lastname: An
authors.forename: Junghoon
authors.initials: J
authors.lastname: Kim
authors.forename: Van-Thuan
authors.initials: VT
authors.lastname: Nguyen
authors.forename: Van-Thao
authors.initials: VT
authors.lastname: Ta
authors.forename: Taisun
authors.initials: T
authors.lastname: Kim
mesh_terms: D052685:Alphapapillomavirus / Q000235:genetics*; D015344:DNA Probes, HPV / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006801:Humans; D057230:Limit of Detection; D025202:Molecular Diagnostic Techniques; D058889:Molecular Typing; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D017422:Sequence Analysis, DNA; D059629:Signal-To-Noise Ratio
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral