title: Isolation and characterization of an oxygen radical absorbance activity peptide from defatted peanut meal hydrolysate and its antioxidant properties.
abstract: Defatted peanut meal hydrolysate (DPMH) was purified using ultrafiltration, gel filtration chromatography, and high-performance liquid chromatography. A tripeptide with strong oxygen radical absorbance capacity (ORAC) was isolated and identified as Tyr-Gly-Ser by ESI-MS/MS. It was then synthesized to measure its antioxidant properties in different systems. The ORAC value of Tyr-Gly-Ser was 3-fold higher than that of glutathione (GSH), and it displayed a stronger protective effect on linoleic acid peroxidation and H(2)O(2)-induced oxidative injury in rat pheochromocytoma line PC12 cells than GSH (p < 0.05). However, Tyr-Gly-Ser showed negligible DPPH radical scavenging activity, reducing power, and no metal chelating ability. The results suggested that Tyr-Gly-Ser displayed antioxidant activity via the hydrogen atom transfer mechanism, and the Tyr at the N-terminal was the hydrogen donor. The ORAC assay was recommended as a reliable and effective method to measure the antioxidant activity in the course of antioxidant peptide isolation.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zheng
authors.forename: Guowan
authors.initials: G
authors.lastname: Su
authors.forename: Jiaoyan
authors.initials: J
authors.lastname: Ren
authors.forename: Longjian
authors.initials: L
authors.lastname: Gu
authors.forename: Lijun
authors.initials: L
authors.lastname: You
authors.forename: Mouming
authors.initials: M
authors.lastname: Zhao
mesh_terms: D010367:Arachis / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D011492:Protein Hydrolysates / Q000737:chemistry* / Q000302:isolation & purification; D017382:Reactive Oxygen Species / Q000737:chemistry
chemical_list: D016166:Free Radical Scavengers; D010455:Peptides; D010936:Plant Extracts; D011492:Protein Hydrolysates; D017382:Reactive Oxygen Species