title: Self-assembled polymeric nanoparticles based on oleic acid-grafted chitosan oligosaccharide: biocompatibility, protein adsorption and cellular uptake.
abstract: Oleic acid-grafted chitosan oligosaccharide (OA-g-CSO) was synthesized to prepare self-assembled nanoparticles by sonication at physiological pH value (7.4). The nanoparticles appeared to be spherical in shape with a diameter of 158.1 ± 64.3 nm. The biocompatibility of OA-g-CSO nanoparticles was evaluated in vitro via MTT assay and hemolysis test. The nanoparticles showed no cytotoxicity to mouse embryo fibroblasts and the hemolysis rates came well within permissible limits (<2 %) in the tested conditions. When incubated with bovine calf serum, the protein adsorption on the surface of OA-g-CSO nanoparticles was concentration-dependent, and the amount of bovine serum albumin was in the highest proportion of the total amount of adsorbed proteins. Cellular uptake rate was evaluated by incubating fluorescence labeled OA-g-CSO nanoparticles with human lung carcinoma cells (A549). OA-g-CSO nanoparticles could be taken up by A549 cells, and the uptake rates increased with incubation time and particle concentration.
authors.affiliation: College of Pharmacy, Binzhou Medical University, Yantai, 264003, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Xi Guang
authors.initials: XG
authors.lastname: Chen
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
authors.forename: Jing Tian
authors.initials: JT
authors.lastname: Han
authors.forename: Xiao Fan
authors.initials: XF
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D051379:Mice; D053758:Nanoparticles; D019301:Oleic Acid / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D011506:Proteins; D019301:Oleic Acid; D048271:Chitosan