title: Small-molecule dissection of brassinosteroid signaling.
abstract: The growth-promoting hormones, the brassinosteroids (BRs), are perceived at the plant cell surface by receptor kinases that transduce the signal to the nucleus by an intracellular cascade of phosphorylation-mediated protein-protein interactions. BR signaling is also regulated by the plant endocytic machinery because the increased endosomal localization of the BR receptor enhances the BR responses. Chemical genetics is a powerful approach to identify new components in redundant signaling networks and to characterize highly dynamic processes, such as endocytosis. Here, we describe a screen in Arabidopsis thaliana seedlings for small molecules that affect hypocotyl elongation under continuous light conditions, indicative for an effect on BR responses. The compounds identified in this screen were used to dissect endomembrane trafficking of the BR receptor, BR INSENSITIVE1, a process that is essential for BR signal transduction.
authors.affiliation: Department of Plant Systems Biology, VIB, Ghent, Belgium.
authors.forename: Mirela-Corina
authors.initials: MC
authors.lastname: Codreanu
authors.forename: Dominique
authors.initials: D
authors.lastname: Audenaert
authors.forename: Long
authors.initials: L
authors.lastname: Nguyen
authors.forename: Tom
authors.initials: T
authors.lastname: Beeckman
authors.forename: Eugenia
authors.initials: E
authors.lastname: Russinova
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism; D060406:Brassinosteroids / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology; D036226:Seedlings / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D060406:Brassinosteroids