title: Applicability and robustness of the hen's egg test for analysis of micronucleus induction (HET-MN): results from an inter-laboratory trial.
abstract: The hen's egg test for analysis of micronucleus formation (HET-MN) was developed several years ago to provide an alternative test system to the in vivo micronucleus test. In order to assess its applicability and robustness, a study was carried out at the University of Osnabrueck (lab A) and at the laboratories of Henkel AG & Co. KGaA (lab B). Following transfer of the method to lab B, a range of test substances that had been pre-tested at lab A, were tested at Henkel: the genotoxins cyclophosphamide, dimethylbenz(a)anthracene, methotrexate, acrylamide, azorubin, N-nitroso-dimethylamine and the non-genotoxins, orange G and isopropyl myristate. In a second phase, additional compounds with known in vivo properties were examined in both labs: the non-genotoxin, ampicillin, the "irrelevant" positives, isophorone and 2,4-dichlorophenol ("irrelevant" means positive in standard in vitro tests, but negative in vivo), the clastogen p-chloroaniline, and the aneugens carbendazim and vinorelbine. All substances were correctly predicted in both labs with respect to their in vivo genotoxic properties, indicating that the HET-MN may have an improved predictivity compared with current standard in vitro test systems. The results support the promising role of the HET-MN assay as a supplement to existing test batteries.
authors.affiliation: University of Osnabrueck, Albrechtstraße 28, 49076 Osnabrück, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Greywe
authors.affiliation: Henkel AG & Co KGaA, Henkelstr 67, 40589 Düsseldorf, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kreutz
authors.affiliation: Henkel AG & Co KGaA, Henkelstr 67, 40589 Düsseldorf, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Banduhn
authors.affiliation: Henkel AG & Co KGaA, Henkelstr 67, 40589 Düsseldorf, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Krauledat
authors.affiliation: Henkel AG & Co KGaA, Henkelstr 67, 40589 Düsseldorf, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Scheel
authors.affiliation: Henkel AG & Co KGaA, Henkelstr 67, 40589 Düsseldorf, Germany.
authors.forename: Klaus R
authors.initials: KR
authors.lastname: Schroeder
authors.affiliation: University of Osnabrueck, Albrechtstraße 28, 49076 Osnabrück, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Wolf
authors.affiliation: Henkel AG & Co KGaA, Henkelstr 67, 40589 Düsseldorf, Germany. Electronic address: kerstin.reisinger@henkel.com.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Reisinger
mesh_terms: D000818:Animals; D002645:Chickens; D004531:Eggs; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D015203:Reproducibility of Results
chemical_list: D009153:Mutagens