title: PDGFBB promotes PDGFRα-positive cell migration into artificial bone in vivo.
abstract: Bone defects caused by traumatic bone loss or tumor dissection are now treated with auto- or allo-bone graft, and also occasionally by artificial bone transplantation, particularly in the case of large bone defects. However, artificial bones often exhibit poor affinity to host bones followed by bony union failure. Thus therapies combining artificial bones with growth factors have been sought. Here we report that platelet derived growth factor bb (PDGFBB) promotes a significant increase in migration of PDGF receptor α (PDGFRα)-positive mesenchymal stem cells/pre-osteoblastic cells into artificial bone in vivo. Growth factors such as transforming growth factor beta (TGFβ) and hepatocyte growth factor (HGF) reportedly inhibit osteoblast differentiation; however, PDGFBB did not exhibit such inhibitory effects and in fact stimulated osteoblast differentiation in vitro, suggesting that combining artificial bones with PDGFBB treatment could promote host cell migration into artificial bones without inhibiting osteoblastogenesis.
authors.affiliation: Department of Dentistry and Oral Surgery, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yoshida
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Iwasaki
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Kawana
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Miyauchi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hoshi
authors.forename: Hiroya
authors.initials: H
authors.lastname: Miyamoto
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Mori
authors.forename: Hiroya
authors.initials: H
authors.lastname: Kanagawa
authors.forename: Eri
authors.initials: E
authors.lastname: Katsuyama
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Fujie
authors.forename: Wu
authors.initials: W
authors.lastname: Hao
authors.forename: Tami
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Yuiko
authors.initials: Y
authors.lastname: Sato
authors.forename: Kana
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Hideo
authors.initials: H
authors.lastname: Morioka
authors.forename: Morio
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Chiba
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Toyama
authors.forename: Taneaki
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyamoto
mesh_terms: D000818:Animals; D000077214:Becaplermin; D001842:Bone and Bones / Q000166:cytology*; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism*
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D020796:Receptor, Platelet-Derived Growth Factor alpha; D048049:Extracellular Signal-Regulated MAP Kinases