title: Comparison and functionalization study of microemulsion-prepared magnetic iron oxide nanoparticles.
abstract: Magnetic iron oxide nanoparticles (MION) for protein binding and separation were obtained from water-in-oil (w/o) and oil-in-water (o/w) microemulsions. Characterization of the prepared nanoparticles have been performed by TEM, XRD, SQUID magnetometry, and BET. Microemulsion-prepared magnetic iron oxide nanoparticles (ME-MION) with sizes ranging from 2 to 10 nm were obtained. Study on the magnetic properties at 300 K shows a large increase of the magnetization ~35 emu/g for w/o-ME-MION with superparamagnetic behavior and nanoscale dimensions in comparison with o/w-ME-MION (10 emu/g) due to larger particle size and anisotropic property. Moringa oleifera coagulation protein (MOCP) bound w/o- and o/w-ME-MION showed an enhanced performance in terms of coagulation activity. A significant interaction between the magnetic nanoparticles and the protein can be described by changes in fluorescence emission spectra. Adsorbed protein from MOCP is still retaining its functionality even after binding to the nanoparticles, thus implying the extension of this technique for various applications.
authors.affiliation: Environmental Microbiology, Royal Institute of Technology (KTH), 106 91 Stockholm, Sweden.
authors.forename: Chuka
authors.initials: C
authors.lastname: Okoli
authors.forename: Margarita
authors.initials: M
authors.lastname: Sanchez-Dominguez
authors.forename: Magali
authors.initials: M
authors.lastname: Boutonnet
authors.forename: Sven
authors.initials: S
authors.lastname: Järås
authors.forename: Concepción
authors.initials: C
authors.lastname: Civera
authors.forename: Conxita
authors.initials: C
authors.lastname: Solans
authors.forename: Gunaratna Rajarao
authors.initials: GR
authors.lastname: Kuttuva
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry; D000077215:Clay; D004655:Emulsions; D005290:Ferric Compounds / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D060306:Magnetometry; D046529:Microscopy, Electron, Transmission; D029588:Moringa oleifera / Q000737:chemistry*; D010316:Particle Size; D010940:Plant Proteins / Q000737:chemistry*; D011485:Protein Binding; D012639:Seeds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014867:Water; D014961:X-Ray Diffraction
chemical_list: D000538:Aluminum Silicates; D004655:Emulsions; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D010940:Plant Proteins; D014867:Water; C000499:ferric oxide; D000077215:Clay