title: The Phyllanthus emblica L. infusion carries immunostimulatory activity in a mouse model.
abstract: BACKGROUND
  Phyllanthus emblica L. (Indian gooseberry, Ma khaam pom) has been an herbal component of Thai traditional recipes proposed to slow down the aging process. A number of methodologies have been employed to investigate the immunological aspects of the so called "anti-aging effects" of P. emblica in a BALB/c mice model.
  
  
  OBJECTIVE
  1) To investigate the immunological efficacy of the anti-aging effects of P. emblica infusion in a BALB/c mice model. 2) To verify the safety for the consumption of P. emblica infusion in BALB/c mice.
  
  
  METHODS
  For in vitro studies, splenocytes were isolated from mice and examined in comparison with the human umbilical endothelial cells, fibroblasts and YAC-1 (mouse lymphoma) cells for proliferative activity upon the exposure to P. emblica infusion. For in vivo studies, mice were orally administered with P. emblica infusion at a dose range of 0, 50, 100, 200 mg/kg BW for 14 days. After the treatments, splenocytes isolated from these mice examined for proliferative and NK cell activities.
  
  
  RESULTS
  For in vitro studies, the infusion of P. emblica could directly drive the proliferation of mouse splenocytes in a dose-dependent manner. The P. emblica infusion itself was already cytotoxic to YAC-1 in the studied dose, while sparing the human umbilical endothelial cells and fibroblasts. For in vivo studies, splenocytes isolated from these mice exhibited dose-dependent proliferative activities. Only the isolated splenocytes from mice ingesting 100 mg/kg BW exhibited an enhancement in NK cell activity.
  
  
  CONCLUSIONS
  P. emblica infusion could drive proliferative activity of splenocyte in vitro and in vivo, with an enhancement in the NK cell-induced cytotoxic activity. The infusion in the aforementioned dose was safe throughout the study.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Sukit
authors.initials: S
authors.lastname: Huabprasert
authors.forename: Kanda
authors.initials: K
authors.lastname: Kasetsinsombat
authors.forename: Kaew
authors.initials: K
authors.lastname: Kangsadalampai
authors.forename: Adisak
authors.initials: A
authors.lastname: Wongkajornsilp
authors.forename: Pravit
authors.initials: P
authors.lastname: Akarasereenont
authors.forename: Uraiwan
authors.initials: U
authors.lastname: Panich
authors.forename: Tawee
authors.initials: T
authors.lastname: Laohapand
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D029842:Phyllanthus emblica / Q000276:immunology*; D010936:Plant Extracts / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology* / Q000276:immunology
chemical_list: D010936:Plant Extracts