title: Catecholaminergic signalling through thymic nerve fibres, thymocytes and stromal cells is dependent on both circulating and locally synthesized glucocorticoids.
abstract: Glucocorticoids have been shown to modulate the expression of noradrenaline metabolizing enzymes and β(2)- and α(1B)-adrenoceptors in a tissue- and cell- specific manner. In the thymus, apart from extensive sympathetic innervation, a regulatory network has been identified that encompasses catecholamine-containing non-lymphoid and lymphoid cells. We examined a putative role of adrenal- and thymus-derived glucocorticoids in modulation of rat thymic noradrenaline levels and adrenoceptor expression. Seven days postadrenalectomy, the thymic levels of mRNAs encoding tyrosine hydroxylase, dopamine β-hydroxylase, monoamine oxidase-A and, consequently, noradrenaline were decreased. Catecholamine content was diminished in autofluorescent nerve fibres (judging by the intensity of fluorescence) and thymocytes (considering HPLC measurements of noradrenaline and the frequency of tyrosine hydroxylase-positive cells), while it remained unaltered in non-lymphoid autofluorescent cells. In addition, adrenalectomy diminished the thymocyte expression of β(2)- and α(1B)-adrenoceptors at both mRNA and protein levels. Administration of ketoconazole (an inhibitor of glucocorticoid synthesis/action; 25 mg kg(-1) day(-1), s.c.) to glucocorticoid-deprived rats increased the thymic levels of tyrosine hydroxylase, dopamine β-hydroxylase and, consequently, noradrenaline. The increased intensity of the autofluorescent cell fluorescence in ketoconazole-treated rats indicated an increase in their catecholamine content, and suggested differential glucocorticoid-mediated regulation of catecholamines in thymic lymphoid and non-lymphoid cells. In addition, ketoconazole increased the thymocyte expression of α(1B)-adrenoceptors. Thus, this study indicates that in the thymus, as in some other tissues, glucocorticoids not only act in concert with cateholamines, but they may modulate catecholamine action by tuning thymic catecholamine metabolism and adrenoceptor expression in a cell-specific manner. Additionally, the study indicates a role of thymus-derived glucocorticoids in this modulation.
authors.affiliation: Immunology Research Centre ‘Branislav Jankovi´c’, Institute of Virology, Vaccines and Sera ‘Torlak’, Belgrade, Serbia.
authors.forename: I
authors.initials: I
authors.lastname: Pilipović
authors.forename: K
authors.initials: K
authors.lastname: Radojević
authors.forename: M
authors.initials: M
authors.lastname: Perišić
authors.forename: D
authors.initials: D
authors.lastname: Kosec
authors.forename: M
authors.initials: M
authors.lastname: Nacka-Aleksić
authors.forename: J
authors.initials: J
authors.lastname: Djikić
authors.forename: G
authors.initials: G
authors.lastname: Leposavić
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism; D000315:Adrenalectomy / Q000379:methods; D000818:Animals; D002395:Catecholamines / Q000235:genetics / Q000378:metabolism*; D004299:Dopamine beta-Hydroxylase / Q000235:genetics / Q000378:metabolism; D005938:Glucocorticoids / Q000235:genetics / Q000378:metabolism*; D007654:Ketoconazole / Q000494:pharmacology; D008297:Male; D008995:Monoamine Oxidase / Q000235:genetics / Q000378:metabolism; D009412:Nerve Fibers / Q000187:drug effects / Q000378:metabolism*; D009638:Norepinephrine / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011941:Receptors, Adrenergic / Q000235:genetics / Q000378:metabolism; D018340:Receptors, Adrenergic, alpha-1 / Q000235:genetics / Q000378:metabolism; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism*; D060168:Thymocytes / Q000187:drug effects / Q000378:metabolism*; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism
chemical_list: D002395:Catecholamines; D005938:Glucocorticoids; D012333:RNA, Messenger; D011941:Receptors, Adrenergic; D018340:Receptors, Adrenergic, alpha-1; D018343:Receptors, Adrenergic, beta-2; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D008995:Monoamine Oxidase; D007654:Ketoconazole; D009638:Norepinephrine