title: Human granulosa luteal cell oxidative phosphorylation function is not affected by age or ovarian response.
abstract: OBJECTIVE
  To safely prepare a functional autologous mitochondrial concentrate (MC) from follicular fluid (FF) cells, and to determine the effect of age and ovarian response on the oxidative phosphorylation (OXPHOS).
  
  
  METHODS
  The nontoxicity of the MC was confirmed in human and mouse oocytes. The OXPHOS function was assessed by measuring the activity of succinate dehydrogenase (SDH) and cytochrome c oxidase (COX), and adenosine triphosphate (ATP) production in comparison with citrate synthase. The integrity of the mitochondrial DNA (mtDNA) was demonstrated by polymerase chain reaction (PCR).
  
  
  METHODS
  Tertiary hospital.
  
  
  METHODS
  A total of 40 patients undergoing IVF of heterogeneous ages and ovarian response.
  
  
  METHODS
  Superovulated 8- to 12-week-old female B(6)C(3)F(1) mice.
  
  
  METHODS
  None.
  
  
  METHODS
  A system for the preparation of functional nontoxic MC was established. The correlation between the mitochondrial mass and function to age and ovarian response was calculated. The integrity of mtDNA was demonstrated.
  
  
  RESULTS
  After injection into mouse oocytes, the MC did not interfere with parthenogenetic development. The MC OXPHOS function was intact. Total activity of SDH and COX was in correlation with the retrieved oocytes number, and in reverse correlation with age. However, after correction to the mitochondrial mass, COX and SDH activities were constant, unaffected by age or ovarian response. The mtDNA was intact in all samples, regardless of age and ovarian response.
  
  
  CONCLUSIONS
  The function of the respiratory chain in mitochondria of FF cells is constant, unaffected by age or ovarian response.
authors.affiliation: IVF Unit, Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel. yoelsh@ekmd.huji.ac.il
authors.forename: Yoel
authors.initials: Y
authors.lastname: Shufaro
authors.forename: Meital
authors.initials: M
authors.lastname: Lebovich
authors.forename: Einat
authors.initials: E
authors.lastname: Aizenman
authors.forename: Chaya
authors.initials: C
authors.lastname: Miller
authors.forename: Alex
authors.initials: A
authors.lastname: Simon
authors.forename: Neri
authors.initials: N
authors.lastname: Laufer
authors.forename: Ann
authors.initials: A
authors.lastname: Saada
mesh_terms: D000328:Adult; D000367:Age Factors; D000818:Animals; D002457:Cell Extracts / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008184:Luteal Cells / Q000378:metabolism* / Q000502:physiology; D008423:Maternal Age; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D008928:Mitochondria / Q000737:chemistry; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D010053:Ovary / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010062:Ovulation Induction; D010085:Oxidative Phosphorylation / Q000187:drug effects; D055815:Young Adult
chemical_list: D002457:Cell Extracts