title: Identification of factors regulating Escherichia coli 2,3-butanediol production by continuous culture and metabolic flux analysis.
abstract: 2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coli was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/ Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Korea.
authors.forename: Mingshou
authors.initials: M
authors.lastname: Lu
authors.forename: Soojin
authors.initials: S
authors.lastname: Lee
authors.forename: Borim
authors.initials: B
authors.lastname: Kim
authors.forename: Changhun
authors.initials: C
authors.lastname: Park
authors.forename: Minkyu
authors.initials: M
authors.lastname: Oh
authors.forename: Kyungmoon
authors.initials: K
authors.lastname: Park
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D053898:Biosynthetic Pathways; D002072:Butylene Glycols / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D007711:Klebsiella pneumoniae / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D002072:Butylene Glycols; D003470:Culture Media; C026978:2,3-butylene glycol