title: Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses.
abstract: Unlike the situation in animals, the final morphology of the plant body is highly modulated by the environment. During Arabidopsis development, intrinsic factors provide the framework for basic patterning processes. CLASS III HOMEODOMAIN LEUCINE ZIPPER (HD-ZIPIII) transcription factors are involved in embryo, shoot and root patterning. During vegetative growth HD-ZIPIII proteins control several polarity set-up processes such as in leaves and the vascular system. We have identified several direct target genes of the HD-ZIPIII transcription factor REVOLUTA (REV) using a chromatin immunoprecipitation/DNA sequencing (ChIP-Seq) approach. This analysis revealed that REV acts upstream of auxin biosynthesis and affects directly the expression of several class II HD-ZIP transcription factors that have been shown to act in the shade-avoidance response pathway. We show that, as well as involvement in basic patterning, HD-ZIPIII transcription factors have a critical role in the control of the elongation growth that is induced when plants experience shade. Leaf polarity is established by the opposed actions of HD-ZIPIII and KANADI transcription factors. Finally, our study reveals that the module that consists of HD-ZIPIII/KANADI transcription factors controls shade growth antagonistically and that this antagonism is manifested in the opposed regulation of shared target genes.
authors.affiliation: Center for Plant Molecular Biology-ZMBP, University of Tübingen, 72076 Tübingen, Germany.
authors.forename: Ronny
authors.initials: R
authors.lastname: Brandt
authors.forename: Mercè
authors.initials: M
authors.lastname: Salla-Martret
authors.forename: Jordi
authors.initials: J
authors.lastname: Bou-Torrent
authors.forename: Thomas
authors.initials: T
authors.lastname: Musielak
authors.forename: Mark
authors.initials: M
authors.lastname: Stahl
authors.forename: Christa
authors.initials: C
authors.lastname: Lanz
authors.forename: Felix
authors.initials: F
authors.lastname: Ott
authors.forename: Markus
authors.initials: M
authors.lastname: Schmid
authors.forename: Thomas
authors.initials: T
authors.lastname: Greb
authors.forename: Martina
authors.initials: M
authors.lastname: Schwarz
authors.forename: Sang-Bong
authors.initials: SB
authors.lastname: Choi
authors.forename: M Kathryn
authors.initials: MK
authors.lastname: Barton
authors.forename: Brenda J
authors.initials: BJ
authors.lastname: Reinhart
authors.forename: Tie
authors.initials: T
authors.lastname: Liu
authors.forename: Marcel
authors.initials: M
authors.lastname: Quint
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Palauqui
authors.forename: Jaime F
authors.initials: JF
authors.lastname: Martínez-García
authors.forename: Stephan
authors.initials: S
authors.lastname: Wenkel
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D019521:Body Patterning; D047369:Chromatin Immunoprecipitation; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D018546:Hypocotyl / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D017403:In Situ Hybridization; D007210:Indoleacetic Acids / Q000032:analysis / Q000378:metabolism; D008027:Light; D009154:Mutation; D010802:Phylogeny; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; C402597:IFL1 protein, Arabidopsis; D007210:Indoleacetic Acids; C430846:KANADI protein, Arabidopsis; D014157:Transcription Factors