title: Characterization and cell behavior of titanium surfaces with PLL/DNA modification via a layer-by-layer technique.
abstract: This study describes the fabrication of two types of multilayered films onto titanium by layer-by-layer (LBL) self-assembly, using poly-L-lysine (PLL) as the cationic polyelectrolyte and deoxyribonucleic acid (DNA) as the anionic polyelectrolyte. The assembling process of each component was studied using atomic force microscopy (AFM) and quartz crystal balance (QCM). Zeta potential of the LBL-coated microparticles was measured by dynamic light scattering. Titanium substrates with or without multilayered films were used in osteoblast cell culture experiments to study cell proliferation, viability, differentiation, and morphology. Results of AFM and QCM indicated the progressive build-up of the multilayered coatings. The surface morphology of three types of multilayered films showed elevations in the nanoscale range. The data of zeta potential showed that the surface terminated with PLL displayed positive charge while the surface terminated with DNA displayed negative charge. The proliferation of osteoblasts on modified titanium films was found to be greater than that on control (p < 0.05) after 3 and 7 days culture, respectively. Alamar blue measurement showed that the PLL/DNA-modified films have higher cell viability (p < 0.05) than the control. Still, the alkaline phosphatase activity assay revealed a better differentiated phenotype on three types of multilayered surfaces compared to noncoated controls. Collectively our results suggest that PLL/DNA were successfully used to surface engineer titanium via LBL technique, and enhanced its cell biocompatibility.
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Gao
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.forename: Xiong
authors.initials: X
authors.lastname: Lu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Wang
authors.forename: Shuxin
authors.initials: S
authors.lastname: Qu
authors.forename: Jie
authors.initials: J
authors.lastname: Weng
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D021621:Imaging, Three-Dimensional; D008422:Materials Testing / Q000379:methods*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D010316:Particle Size; D011107:Polylysine / Q000494:pharmacology*; D058525:Quartz Crystal Microbalance Techniques; D051381:Rats; D055672:Static Electricity; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000494:pharmacology*
chemical_list: D011107:Polylysine; D004247:DNA; D014025:Titanium; D000469:Alkaline Phosphatase