title: Effect of sericin on preimplantation development of bovine embryos cultured individually.
abstract: The silk protein sericin has been identified as a potent antioxidant in mammalian cells. This study was conducted to examine the effects of sericin on preimplantation development and quality of bovine embryos cultured individually. When two-cell-stage embryos were cultured individually for 7 days in CR1aa medium supplemented with 0, 0.1, 0.5, or 1% sericin, rates of total blastocyst formation and development to expanded blastocysts from embryos cultured with 0.5% sericin were higher (P < 0.05) than those from embryos cultured with 0 or 1% sericin. When embryos were cultured individually for 7 days in the CR1aa medium supplemented with 0 or 0.5% sericin under two oxidative stress conditions (50 or 100 μm H(2)O(2)), the addition of sericin significantly improved the blastocyst formation rate of embryos exposed to 100 μm H(2)O(2). However, the protective effect of sericin was not observed in development of embryos exposed to 50 μm H(2)O(2). When embryos were exposed to 100 μm H(2)O(2) during culture, the DNA fragmentation index of total blastocysts from embryos cultured with 0.5% sericin was lower than blastocysts derived from embryos cultured without sericin (4.4 vs. 6.8%; P < 0.01). In conclusion, the addition of 0.5% sericin to in vitro culture medium improved preimplantation development and quality of bovine embryos cultured individually by preventing oxidative stress.
authors.affiliation: Laboratory of Animal Reproduction, Department of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Isobe
authors.forename: Y
authors.initials: Y
authors.lastname: Ikebata
authors.forename: T
authors.initials: T
authors.lastname: Onitsuka
authors.forename: M
authors.initials: M
authors.lastname: Wittayarat
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Taniguchi
authors.forename: T
authors.initials: T
authors.lastname: Otoi
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000187:drug effects*; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D046149:Embryo Culture Techniques / Q000379:methods / Q000662:veterinary; D047108:Embryonic Development / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007206:Individuality; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D011786:Quality Control; D047030:Sericins / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D047030:Sericins; D006861:Hydrogen Peroxide