title: NMR-based isotopologue profiling of microbial carotenoids.
abstract: C-Isotopologue profiling is a powerful tool to determine on a quantitative basis the biosynthetic origin of carotenoids in microorganisms. To this aim, the carotenoid-producing microorganism is grown in medium containing (13)C-labeled glucose. After growth, the (13)C-isotopologue distribution in a given biosynthetic carotenoid is determined by quantitative NMR spectroscopy. The labeling pattern provides a fingerprint of processes involved in the metabolism of glucose and the formation of the carotenoid. For example, the (13)C-profile shows whether the isoprenoid precursors, isopentenyl diphosphate and dimethylallyl diphosphate, are formed by the mevalonate or the non-mevalonate pathway. The labeling data also specify the pathways of glucose utilization, e.g., via the Entner-Doudoroff pathway or glycolysis. The method is exemplified with the analysis of zeaxanthin biosynthesis in the Alphaproteobacterium, Paracoccus zeaxanthinifaciens.
authors.affiliation: Lehrstuhl für Biochemie, Technische Universität München, München, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Eylert
authors.forename: Adelbert
authors.initials: A
authors.lastname: Bacher
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Eisenreich
mesh_terms: D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D002338:Carotenoids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*
chemical_list: D002338:Carotenoids