title: Development of an immunochromatographic assay for rapid detection of AAC(6')-Ib-producing Pseudomonas aeruginosa.
abstract: To detect aminoglycoside 6'-N-acetyltransferase-Ib [AAC(6')-Ib]-producing, Pseudomonas aeruginosa isolates which are a frequent cause of nosocomial infections in Japan, an immunochromatographic assay was developed using two kinds of monoclonal antibodies (mAbs) recognizing AAC(6')-Ib. The results of the assessment were fully consistent with those of aac(6')-Ib PCR analyses.
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tada
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.forename: Masashi
authors.initials: M
authors.lastname: Tanaka
authors.forename: Kenji
authors.initials: K
authors.lastname: Narahara
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shimojima
authors.forename: Tomoe
authors.initials: T
authors.lastname: Kitao
authors.forename: Kayo
authors.initials: K
authors.lastname: Shimada
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
mesh_terms: D000123:Acetyltransferases / Q000032:analysis*; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007564:Japan; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000123:Acetyltransferases; C056532:aminoglycoside N(6')-acetyltransferase