title: Lentiviral expression of anti-microRNAs targeting miR-27a inhibits proliferation             and invasiveness of U87 glioma cells.
abstract: Glioma is a highly fatal malignant disease and its treatment options are limited. microRNAs represent a novel target for the treatment of cancer. In the present study, we used a lentiviral vector to stably express anti-microRNAs targeting the oncogenic miR-27a in U87 glioma cells. The stable expression of anti-miR-27a significantly reduced the proliferation and increased the accumulation of U87 cells in the sub-G1 phase as determined by Cell Counting kit-8 (CCK-8) assays and flow cytometry, respectively. Results from the Matrigel transwell assay also indicated that the inhibition of miR-27a substantially impaired the invasiveness of U87 cells. By combining bioinformatic and proteomic approaches, we identified the mRNAs of 8 proteins upregulated in anti-miR-27a-expressing U87 cells as putative direct targets of miR-27a. Collectively, these data suggest that the lentiviral expression of anti-miR-27a is a feasible approach for the suppression of malignant phenotypes of glioma cells.
authors.affiliation: Department of Neurosurgery, Wuxi People's Hospital of Nanjing Medical University, Wuxi 214023, PR China.
authors.forename: Su Y
authors.initials: SY
authors.lastname: Feng
authors.forename: Chang G
authors.initials: CG
authors.lastname: Dong
authors.forename: William K K
authors.initials: WK
authors.lastname: Wu
authors.forename: Xiao J
authors.initials: XJ
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Qiao
authors.forename: Jun F
authors.initials: JF
authors.lastname: Shao
mesh_terms: D000465:Algorithms; D017209:Apoptosis; D002452:Cell Count; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003001:Cloning, Molecular; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D005434:Flow Cytometry; D016193:G1 Phase; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D057809:HEK293 Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D010641:Phenotype; D040901:Proteomics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014162:Transfection; D015854:Up-Regulation
chemical_list: C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D012334:RNA, Neoplasm