title: Evaluation of the cytotoxicity of Satureja spicigera and its main compounds.
abstract: Satureja spicigera (Lamiaceae) grows wildly in Northwest of Iran. In this study, bioassay-guided isolation and identification of the main compounds has been reported using various chromatographic methods and comparison of their spectral data with those reported in the literature. Brine shrimp lethality and four cancerous cell lines HT29/219, Caco(2), NIH-3T3, and T47D were used for cytotoxicity evaluations. From the aerial parts of S. spicigera, nine known compounds including two flavanones, 5,7,3',5'-tetrahydroxy flavanone (8) and 5,4'-dihydroxy-3'-methoxyflavanone-7-(6''-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside (9), one dihydrochalcone, nubigenol (7), together with thymoquinone (1), thymol (2), carvacrol (3), β-sitosterol (4), ursolic acid (5) and oleanolic acid (6) were identified. Among the isolated chalcone and flavanones, compound 8 was effective against Artemia salina larva (LC(50)= 2 μg/mL) and only the compound 9 demonstrated IC(50) value of 98.7 μg/mL on the T47D (human, breast, ductal carcinoma). Other compounds did not show significant inhibition of the cell growth.
authors.affiliation: Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ahmad Reza
authors.initials: AR
authors.lastname: Gohari
authors.forename: Seyed Nasser
authors.initials: SN
authors.lastname: Ostad
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Moradi-Afrapoli
authors.forename: Maryam
authors.initials: M
authors.lastname: Malmir
authors.forename: Shohreh
authors.initials: S
authors.lastname: Tavajohi
authors.forename: Hassan
authors.initials: H
authors.lastname: Akbari
authors.forename: Soodabeh
authors.initials: S
authors.lastname: Saeidnia
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000633:toxicity*; D001156:Artemia / Q000187:drug effects; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D044950:Flavanones / Q000633:toxicity*; D035264:Flowers / Q000737:chemistry; D019073:HT29 Cells; D006801:Humans; D007928:Lethal Dose 50; D051379:Mice; D041681:NIH 3T3 Cells; D010936:Plant Extracts / Q000633:toxicity*; D031362:Satureja / Q000737:chemistry*; D018675:Toxicity Tests
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D044950:Flavanones; D010936:Plant Extracts