title: A 15 nucleotide deletion mutation in coding region of the RIG-I lowers grass carp (Ctenopharyngodon idella) resistance to grass carp reovirus.
abstract: RIG-I (Retinoic acid-inducible gene I) is a pivotal receptor that detects numerous RNA and DNA viruses and plays crucial roles in the induction of type I interferons. In the present study, a deletion mutation in CiRIG-I (Ctenopharyngodon idella RIG-I) coding region was detected, its association with resistance/susceptibility to grass carp reovirus (GCRV) was examined, and possible mechanism was analyzed. A 15-bp deletion mutation was found, and the mutation results in a deletion of five amino acids. To investigate the genotypes and alleles, the relevant PCR products were electrophoresed on 2.5% agarose gel. Three genotypes and two alleles were discovered. The general allele was named as A and the deletion mutation allele was named as B. The deletion mutation cancels a predicted phosphorylation site and changes the secondary structure and the probability of peroxisomal targeting signal 1 in CiRIG-I. To explore the correlation between these genotypes and the resistance of grass carp to GCRV, a challenge experiment was carried out. The cumulative mortality in genotype AA (40.70%) and AB (52.73%) was significantly lower than that in genotype BB (71.43%) (P = 0.032). The result demonstrated that genotype AA and AB were resistant to GCRV, while genotype BB was susceptible. The 15-bp deletion mutation lowers the resistance of grass carp to GCRV. This result might provide a potential genetic marker for further investigation of selective breeding of resistant grass carp to GCRV.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, China.
authors.forename: Quanyuan
authors.initials: Q
authors.lastname: Wan
authors.forename: Jianguo
authors.initials: J
authors.lastname: Su
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.forename: Lijun
authors.initials: L
authors.lastname: Chen
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Chen
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000235:genetics*; D005393:Fish Diseases / Q000235:genetics* / Q000401:mortality; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D005838:Genotype; D008969:Molecular Sequence Data; D012087:Reoviridae; D012088:Reoviridae Infections / Q000235:genetics / Q000401:mortality / Q000662:veterinary*; D017384:Sequence Deletion / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D014157:Transcription Factors