title: Structural and biochemical characterization of native and recombinant single insulin-like growth factor-binding domain protein (SIBD-1) from the Central American hunting spider Cupiennius salei (Ctenidae).
abstract: Cupiennius salei single insulin-like growth factor binding domain protein (SIBD-1) is an 8.6 kDa Cys-, Pro-, and Gly-rich protein, discovered in the hemocytes of the Central American hunting spider Cupiennius salei. SIBD-1 exhibits high sequence similarity to the N-terminal domain of the insulin-like growth factor-binding protein superfamily and has been reported to play an important role in the spider's immune system. Here, the recombinant expression and the elucidation of the three-dimensional structure of recombinant SIBD-1 and the characterization of the sugar moiety at Thr2 of native SIBD-1 is described in detail.
authors.affiliation: Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland.
authors.forename: Christian
authors.initials: C
authors.lastname: Trachsel
authors.forename: Christine
authors.initials: C
authors.lastname: Widmer
authors.forename: Urs
authors.initials: U
authors.lastname: Kämpfer
authors.forename: Claudia
authors.initials: C
authors.lastname: Bühr
authors.forename: Tommy
authors.initials: T
authors.lastname: Baumann
authors.forename: Lucia
authors.initials: L
authors.lastname: Kuhn-Nentwig
authors.forename: Stefan
authors.initials: S
authors.lastname: Schürch
authors.forename: Johann
authors.initials: J
authors.lastname: Schaller
authors.forename: Ulrich
authors.initials: U
authors.lastname: Baumann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry* / Q000378:metabolism; D002489:Central America; D018969:Insulin-Like Growth Factor Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism; D013112:Spiders
chemical_list: D060829:Arthropod Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D011994:Recombinant Proteins