title: Investigation of the component of Lycopodium serratum extract that inhibits proliferation and mediates apoptosis of human HL-60 leukemia cells.
abstract: In this study, we investigate a plant commonly used in herbal medicines, Lycopodium serratum, which is believed to have anti-cancer properties. An alcoholic extract of L. serratum (LSE) was investigated for its ability to induce apoptosis in cultured human promyelocytic leukemia HL-60 cells. Treatment of HL-60 cells with various concentrations of LSE (6-100 μg/mL) resulted in a sequence of events characteristic of apoptosis, including loss of cell viability, morphological changes, and increased sub-G(1) DNA content. Serratenediol (SE), a known biologically active agent, was isolated from MC fraction of LSE and was able to demonstrate significant and dose-dependent growth inhibitory effects on HL-60 cells. Similar to the effects observed with the crude LSE, the SE-related effects included the formation of apoptotic bodies and fragmented DNA, as well as the accumulation of DNA in the sub-G(1) phase of the cell cycle. Analysis of the mechanism of these events indicated that SE treated cells had an increased ratio of Bax/Bcl-xL, released the cytochrome c, activated caspase-9, -3, and cleaved poly-ADP-ribose polymerase (PARP); these observations are hallmarks of apoptotic events. Thus, the results suggest that SE can induce apoptosis via regulating the ratio of Bax/Bcl-xL in HL-60 cell lines.
authors.affiliation: Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 699-943, Republic of Korea.
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Ham
authors.forename: Weon-Jong
authors.initials: WJ
authors.lastname: Yoon
authors.forename: Soo-Yeong
authors.initials: SY
authors.lastname: Park
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Jung
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
authors.forename: W A J P
authors.initials: WA
authors.lastname: Wijesinghe
authors.forename: Sung-Myung
authors.initials: SM
authors.lastname: Kang
authors.forename: Kil-Nam
authors.initials: KN
authors.lastname: Kim
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology; D032502:Lycopodium / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts