title: A tetracycline inducible expression vector for Corynebacterium glutamicum allowing tightly regulable gene expression.
abstract: Here we report on the construction of a tetracycline inducible expression vector that allows a tightly regulable gene expression in Corynebacterium glutamicum which is used in industry for production of small molecules such as amino acids. Using the green fluorescent protein (GFP) as a reporter protein we show that this vector, named pCLTON1, is characterized by tight repression under non-induced conditions as compared to a conventional IPTG inducible expression vector, and that it allows gradual GFP synthesis upon gradual increase of anhydrotetracycline addition.
authors.affiliation: Institut für Bio- und Geowissenschaften, IBG1: Biotechnologie, Forschungszentrum Jülich GmbH, D-52425 Jülich, Germany. f.lausberg@fz-juelich.de
authors.forename: Frank
authors.initials: F
authors.lastname: Lausberg
authors.forename: Ava Rebecca
authors.initials: AR
authors.lastname: Chattopadhyay
authors.forename: Antonia
authors.initials: A
authors.lastname: Heyer
authors.forename: Lothar
authors.initials: L
authors.lastname: Eggeling
authors.forename: Roland
authors.initials: R
authors.lastname: Freudl
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D048230:Corynebacterium glutamicum / Q000187:drug effects* / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D023061:Gene Order; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics*; D009875:Operator Regions, Genetic; D011401:Promoter Regions, Genetic; D013752:Tetracycline / Q000494:pharmacology*; D015534:Trans-Activators / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D015534:Trans-Activators; D013752:Tetracycline