title: Kidins220/ARMS interacts with Pdzrn3, a protein containing multiple binding domains.
abstract: We report the identification of a novel partner of Kidins220/ARMS (Kinase D-interacting substrate of 220 kDa/Ankyrin Repeat-rich Membrane Spanning) an adaptor of neurotrophin receptors playing crucial roles during neurogenesis. Screening a phage display library of brain cDNA products we found that D. rerio Pdzrn3, a protein containing RING-finger and PDZ-domains, interacts with Kidins220/ARMS through its first PDZ-domain. Both zebrafish proteins share high homology with the corresponding mammalian proteins and both genes are developmentally expressed in neural districts where early neurogenesis occurs. The interaction was also confirmed by biochemical assays and by co-localization at the tips of growing neurites of PC12 cells induced with nerve growth factor.
authors.affiliation: Department of Biology, University of Pisa, SS12 Abetone-Brennero 4, 56127 Pisa, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Andreazzoli
authors.forename: Gaia
authors.initials: G
authors.lastname: Gestri
authors.forename: Elena
authors.initials: E
authors.lastname: Landi
authors.forename: Beatrice
authors.initials: B
authors.lastname: D'Orsi
authors.forename: Manuela
authors.initials: M
authors.lastname: Barilari
authors.forename: Angela
authors.initials: A
authors.lastname: Iervolino
authors.forename: Marianna
authors.initials: M
authors.lastname: Vitiello
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Wilson
authors.forename: Luciana
authors.initials: L
authors.lastname: Dente
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D019295:Computational Biology; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D016716:PC12 Cells; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051381:Rats; D044767:Ubiquitin-Protein Ligases; D029961:Zebrafish Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C482591:KIDINS220 protein, human; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D029961:Zebrafish Proteins; C485992:PDZRN3 protein, human; D044767:Ubiquitin-Protein Ligases