title: Structural alterations of the mucosa stroma in the Barrett's esophagus metaplasia-dysplasia-adenocarcinoma sequence.
abstract: OBJECTIVE
  Accumulating evidence suggests that the extracellular matrix play important roles in intercellular communications and contribute to the development of a number of diseases, including diseases of the gastrointestinal tract. The present study examined the structural characteristics and alterations of the extracellular matrix of the mucosa stroma in the Barrett's esophagus metaplasia-dysplasia-adenocarcinoma sequence.
  
  
  METHODS
  A total of 41 esophageal tissue specimens (15 esophageal adenocarcinoma, 10 Barrett's esophagus intestinal metaplasia, seven dysplasia and nine normal esophagus) were studied. The present study used transmission electron microscopy and computerized quantitative electron-microscopic analysis in order to investigate the characteristics of the extracellular matrix of the mucosa.
  
  
  RESULTS
  The study revealed that marked structural alterations of the mucosa stroma, relating to changes in the distribution and appearance of collagen fibers as well as to changes in numbers of matrix microvesicles, occur in Barrett's esophagus and esophageal adenocarcinoma. It was found that there were 3.1 times more microvesicles in the stroma in Barrett's esophagus than in the stroma of the normal esophagus (P<0.0001) and that there were 5.8 times more microvesicles in esophageal adenocarcinoma than in the normal esophagus (P<0.0001). There were 1.9 times more microvesicles in esophageal adenocarcinoma than in Barrett's esophagus (P=0.0043).
  
  
  CONCLUSIONS
  The study demonstrates distinctive alterations of the mucosa stroma extracellular matrix in the metaplasia-dysplasia-adenocarcinoma sequence. The findings suggest that the redistribution of collagen fibers and increases in numbers of matrix microvesicles may play roles in the formation of specialized intestinal metaplasia and the development of adenocarcinoma.
authors.affiliation: St. Vincent's Centre for Applied Medical Research and Department of Surgery, St Vincent's Hospital Sydney, University of New South Wales, Sydney, Australia. y.bobryshev@unsw.edu.au
authors.forename: Yuri V
authors.initials: YV
authors.lastname: Bobryshev
authors.forename: Murray C
authors.initials: MC
authors.lastname: Killingsworth
authors.forename: Reginald V N
authors.initials: RV
authors.lastname: Lord
mesh_terms: D000230:Adenocarcinoma / Q000648:ultrastructure*; D001471:Barrett Esophagus / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000648:ultrastructure; D003094:Collagen / Q000648:ultrastructure; D004938:Esophageal Neoplasms / Q000648:ultrastructure*; D004947:Esophagus / Q000473:pathology* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000648:ultrastructure*; D006801:Humans; D008679:Metaplasia / Q000473:pathology; D046529:Microscopy, Electron, Transmission; D009092:Mucous Membrane / Q000648:ultrastructure*; D017154:Stromal Cells / Q000648:ultrastructure
chemical_list: D003094:Collagen