title: Evidence of antiapoptotic properties of Pleurotus florida lectin against chronic arsenic toxicity in renal cells of rats.
abstract: Chronic arsenic exposure results in toxicity in humans and causes many toxicologic manifestations. Apoptosis was measured by cell adhesion, morphologic alterations, cell proliferation, terminal deoxyuridine triphosphate nick-end labeling (TUNEL), and caspase-3/CPP32 fluorometric protease assay. Results of the present study suggested that arsenic administration in rats caused apoptosis by elevating morphologic alterations, TUNEL-positive nuclei, caspase-3 activity, and DNA damage and by reducing cell adhesion and cell proliferation in a time-dependent manner. The apoptosis in renal cells of arsenic-exposed rats reverted to normal values after coadministration of mushroom lectin. This study provided significant evidence that Pleurotus florida lectin has an antiapoptotic property by protecting from arsenic-induced toxicity. The beneficial effect of Pleurotus florida lectin was proportional to its duration of exposure. This finding might be of therapeutic benefit in people suffering from chronic exposure to arsenic from natural sources, a global problem that is especially relevant to millions of people on the Indian subcontinent.
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, Kolkata, India.
authors.forename: Tanmoy
authors.initials: T
authors.lastname: Rana
authors.forename: Asit Kumar
authors.initials: AK
authors.lastname: Bera
authors.forename: Debasis
authors.initials: D
authors.lastname: Bhattacharya
authors.forename: Subhashree
authors.initials: S
authors.lastname: Das
authors.forename: Diganta
authors.initials: D
authors.lastname: Pan
authors.forename: Subrata Kumar
authors.initials: SK
authors.lastname: Das
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001151:Arsenic / Q000494:pharmacology / Q000633:toxicity*; D020261:Arsenic Poisoning / Q000517:prevention & control*; D053148:Caspase 3 / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002908:Chronic Disease; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D037102:Lectins / Q000494:pharmacology* / Q000627:therapeutic use*; D008297:Male; D023421:Models, Animal; D020076:Pleurotus; D051381:Rats; D017208:Rats, Wistar
chemical_list: D037102:Lectins; D053148:Caspase 3; D001151:Arsenic