title: Transcriptome analysis using a high-density oligomicroarray under drought stress in various genotypes of cassava: an important tropical crop.
abstract: Cassava is an important crop that provides food security and income generation in many tropical countries and is known for its adaptability to various environmental conditions. Despite its global importance, the development of cassava microarray tools has not been well established. Here, we describe the development of a 60-mer oligonucleotide Agilent microarray representing ∼20,000 cassava genes and how it can be applied to expression profiling under drought stress using three cassava genotypes (MTAI16, MECU72 and MPER417-003). Our results identified about 1300 drought stress up-regulated genes in cassava and indicated that cassava has similar mechanisms for drought stress response and tolerance as other plant species. These results demonstrate that our microarray is a useful tool for analysing the cassava transcriptome and that it is applicable for various cassava genotypes.
authors.affiliation: Plant Genomic Network Research Team, RIKEN Plant Science Center, Tsurumi-ku, Yokohama, Kanagawa, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Utsumi
authors.forename: Maho
authors.initials: M
authors.lastname: Tanaka
authors.forename: Taeko
authors.initials: T
authors.lastname: Morosawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kurotani
authors.forename: Takuhiro
authors.initials: T
authors.lastname: Yoshida
authors.forename: Keiichi
authors.initials: K
authors.lastname: Mochida
authors.forename: Akihiro
authors.initials: A
authors.lastname: Matsui
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Umemura
authors.forename: Manabu
authors.initials: M
authors.lastname: Ishitani
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
mesh_terms: D018556:Crops, Agricultural / Q000235:genetics*; D055864:Droughts; D020869:Gene Expression Profiling; D014644:Genetic Variation; D018745:Genome, Plant; D005838:Genotype; D002365:Manihot / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D059467:Transcriptome / Q000235:genetics*