title: A Preliminary Investigation of NSCL/P Plasma and Urine in Guizhou Province in China Using NMR-Based Metabonomics.
abstract: Objective : To assess the feasibility of metabonomics in clinical studies. This is a pilot study introducing nuclear magnetic resonance (NMR)-based metabonomics to elucidate and compare the metabolism of patients with nonsyndromic cleft lip and/or palate (NSCL/P) and children without orofacial clefts. Methods : High-resolution (1)H NMR spectroscopy was performed on plasma and urine samples obtained from NSCL/P and healthy children. The (1)H NMR spectra were further analyzed with principal component analysis. Results : Compared to the control group, the level of low-molecular-weight metabolites in plasma such as asparagine was higher in NSCL/P patients, while arginine, lysine, acetate, lactate, proline, glutamine, pyruvate, creatinine, choline, and β-glucose were lower. The carnitine, citrate, and formate excretion in urine appeared to be higher in the healthy children, while the NSCL/P group excreted higher concentrations of aspartic acid and phenylalanine in urine. Conclusion : The present study clearly demonstrated the great potential of NMR-based metabonomics in elucidating NSCL/P plasma metabolism and the possible application of this technology in clinical diagnosis and screening.
authors.forename: Huang Guang
authors.initials: HG
authors.lastname: Lei
authors.forename: Luo
authors.initials: L
authors.lastname: Hong
authors.forename: Song Ju
authors.initials: SJ
authors.lastname: Kun
authors.forename: Yin Xin
authors.initials: YX
authors.lastname: Hai
authors.forename: Wang Ya
authors.initials: WY
authors.lastname: Dong
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ke
authors.forename: Xu
authors.initials: X
authors.lastname: Ping
authors.forename: Chen
authors.initials: C
authors.lastname: Hao
mesh_terms: D016022:Case-Control Studies; D002681:China; D006801:Humans; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D010865:Pilot Projects