title: [Study on genetic transformation of antigen G3VP7 gene of human rotavirus in peanut].
abstract: In order to increase the expression level of target gene and to simplify the purifying process of separation and purification, we performed the transgenetic research of antigen VP7 gene into peanut via Agrobacterium tumefaciens. The plant binary expression vector is pBOG3VP7 harboring fusion gene oleosin-vp7, which is promoted by ole-promoter. Cotyledon nodes were used as transformation recipients. Transformed individuals were obtained through selection on medium containing 125 mg L-1 Kan. Integration of transgenes was assessed by PCR amplification and PCR-Southern blot hybridization. Taking pBOG3VP7 plasmid as positive control, non-transformed peanut as negative control. 6 plants among 11 plants grown up through seletion medium were detected by PCR and the rate of positive plants is 54.5%. PCR positive plants were further analysed by PCR-Southern blot hybridization. The results showed that 3 plants have DNA bloting bands. The results also showed that the foreign gene was integrated into genome of transformed peanuts. Elevated expression of rotavirus VP7 antigen in transgenic peanuts was a critical factor in the development of efficient and cheap plant oral vaccine.
authors.affiliation: Research Center of Molecular Biology, Inner Mongolia Medical College, Hohhot 010059, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Jia
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Xue
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Shaowei
authors.initials: S
authors.lastname: Li
authors.forename: Dehu
authors.initials: D
authors.lastname: Liu
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics*; D010367:Arachis / Q000235:genetics* / Q000378:metabolism; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D012401:Rotavirus / Q000235:genetics / Q000276:immunology*; D014170:Transformation, Genetic; D014614:Vaccines, Synthetic
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus; D014614:Vaccines, Synthetic