title: Intranasal inoculation with an adenovirus vaccine encoding ten repeats of Aβ3-10 reduces AD-like pathology and cognitive impairment in Tg-APPswe/PSEN1dE9 mice.
abstract: To develop a safe and efficient vaccine for AD treatment, we constructed an adenovirus vector vaccine encoding ten repeats of Aβ3-10 and CpG motif as a molecular adjuvant. We demonstrated that therapeutic immunization with Ad-10×Aβ3-10-CpG elicits Aβ3-10 specific Th2-polarized immune response with high titers of anti-Aβ antibodies in APPswe/PSEN1dE9 mice, which in turn reduced Aβ deposits in brains and cognitive impairment. In addition, Ad-10×Aβ3-10-CpG reduced astrocytosis without increasing the incidence of microhemorrhage. Our findings of this study raise the possibility that the adenovirus vaccine Ad-10×Aβ3-10-CpG would be a safe and effective alternative for AD immunotherapy.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of China Medical University, Shenyang, Liao Ning Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Zong
authors.forename: Xiao-Na
authors.initials: XN
authors.lastname: Xing
authors.forename: Rong
authors.initials: R
authors.lastname: Guo
authors.forename: Tong-Zi
authors.initials: TZ
authors.lastname: Jiang
authors.forename: Sha
authors.initials: S
authors.lastname: Sha
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Cao
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000281:Administration, Intranasal; D000544:Alzheimer Disease / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D023582:Alzheimer Vaccines / Q000008:administration & dosage* / Q000276:immunology; D016229:Amyloid beta-Peptides / Q000008:administration & dosage* / Q000276:immunology*; D000818:Animals; D001921:Brain / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D003072:Cognition Disorders / Q000209:etiology / Q000517:prevention & control*; D018899:CpG Islands / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors; D007150:Immunohistochemistry; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D023582:Alzheimer Vaccines; D016229:Amyloid beta-Peptides; D010446:Peptide Fragments