title: Predicted Relative Metabolomic Turnover (PRMT): determining metabolic turnover from a coastal marine metagenomic dataset.
abstract: BACKGROUND
  The world's oceans are home to a diverse array of microbial life whose metabolic activity helps to drive the earth's biogeochemical cycles. Metagenomic analysis has revolutionized our access to these communities, providing a system-scale perspective of microbial community interactions. However, while metagenome sequencing can provide useful estimates of the relative change in abundance of specific genes and taxa between environments or over time, this does not investigate the relative changes in the production or consumption of different metabolites.
  
  
  RESULTS
  We propose a methodology, Predicted Relative Metabolic Turnover (PRMT) that defines and enables exploration of metabolite-space inferred from the metagenome. Our analysis of metagenomic data from a time-series study in the Western English Channel demonstrated considerable correlations between predicted relative metabolic turnover and seasonal changes in abundance of measured environmental parameters as well as with observed seasonal changes in bacterial population structure.
  
  
  CONCLUSIONS
  The PRMT method was successfully applied to metagenomic data to explore the Western English Channel microbial metabalome to generate specific, biologically testable hypotheses. Generated hypotheses linked organic phosphate utilization to Gammaproteobactaria, Plantcomycetes, and Betaproteobacteria, chitin degradation to Actinomycetes, and potential small molecule biosynthesis pathways for Lentisphaerae, Chlamydiae, and Crenarchaeota. The PRMT method can be applied as a general tool for the analysis of additional metagenomic or transcriptomic datasets.
authors.affiliation: Argonne National Laboratory, 9700, S, Cass Ave, Argonne, Illinois, USA. plarsen@anl.gov.
authors.forename: Peter E
authors.initials: PE
authors.lastname: Larsen
authors.forename: Frank R
authors.initials: FR
authors.lastname: Collart
authors.forename: Dawn
authors.initials: D
authors.lastname: Field
authors.forename: Folker
authors.initials: F
authors.lastname: Meyer
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Keegan
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Henry
authors.forename: John
authors.initials: J
authors.lastname: McGrath
authors.forename: John
authors.initials: J
authors.lastname: Quinn
authors.forename: Jack A
authors.initials: JA
authors.lastname: Gilbert