title: Protein identification on archived 2-D gels.
abstract: Because of the availability of genome information combined with proteomics techniques, it is possible to determine the identity of a protein which had been isolated many years ago on a two-dimensional gel electrophoresis and stored in a dry state as a data archive. The protocol described in this chapter will assist researchers who want to know the identity of a protein separated decades ago when no techniques were available to determine the identity of the protein.
authors.affiliation: University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. hiroyuki-matsumoto@ouhsc.edu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Takemori
authors.forename: Naoka
authors.initials: N
authors.lastname: Komori
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods*; D011309:Preservation, Biological; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D059748:Proteolysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014357:Trypsin / Q000737:chemistry
chemical_list: D010446:Peptide Fragments; D011506:Proteins; D014357:Trypsin