title: Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling.
abstract: The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homoduplex or heteroduplex DNA. This approach allows stabilization of various transient MutS-DNA complexes and will enable their structural and functional analysis.
authors.affiliation: Institute for Biochemistry, FB 08, Justus Liebig University, Heinrich-Buff-Ring 58, D-35392 Giessen, Germany.
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Heinze
authors.forename: Svetlana
authors.initials: S
authors.lastname: Sekerina
authors.forename: Ines
authors.initials: I
authors.lastname: Winkler
authors.forename: Christian
authors.initials: C
authors.lastname: Biertümpfel
authors.forename: Tatiana S
authors.initials: TS
authors.lastname: Oretskaya
authors.forename: Elena
authors.initials: E
authors.lastname: Kubareva
authors.forename: Peter
authors.initials: P
authors.lastname: Friedhoff
mesh_terms: D020137:Base Pair Mismatch; D053843:DNA Mismatch Repair; D004269:DNA, Bacterial / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D051719:MutS DNA Mismatch-Binding Protein / Q000737:chemistry / Q000378:metabolism*; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D009692:Nucleic Acid Heteroduplexes; D051719:MutS DNA Mismatch-Binding Protein