title: The pharmacokinetics and metabolism study of sodium 7,4' -oxo-acetic acid daidzein in rat.
abstract: OBJECTIVE
  To Study the pharmacokinetics and metabolism of Sodium 7,4' -oxo-acetic acid daidzein in rat.
  
  
  METHODS
  Sodium 7,4' -oxo-acetic acid daidzein was determined by reversed-phase HPLC (column: German CenturySIL BDS C18 5 μm silica, 200 mm x 4.6 mm i.d; fluid: methanol-water-85% phosphoric acid(57:43:0.05, v/v/v)), with sodium benzoic acid as an internal standard. Biological samples were extracted with acetonitrile.
  
  
  RESULTS
  The calibration curve was linear over the range of 1.0-1000 μg/ml in rat plasma, urine and feces. The average extraction recoveries were 73.3% (plasma), 73.9% (urine) and 83.7% (feces) respectively and the intra-day and inter-day precisions were less than 8.34%. The assay was applied to the analysis of samples from a pharmacokinetic study. The absolute bioavailability of oral administration was 3.07%. DZ I original compound determined in 6h urine was 32.32%, in 24h urine was 32.71%, in 24h feces was 22.96%.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, No.103, Wenhua Road, Shenyang 110016, PR China. xiaohu6408_cn@sina.com
authors.forename: Shujun
authors.initials: S
authors.lastname: Wang
authors.forename: Ruixue
authors.initials: R
authors.lastname: Gao
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yi
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.forename: Chengyu
authors.initials: C
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Cheng
mesh_terms: D000085:Acetates / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry; D007529:Isoflavones / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: C581548:7,4' -oxo-acetic acid daidzein; D000085:Acetates; D007529:Isoflavones