title: Dioxinodehydroeckol inhibits melanin synthesis through PI3K/Akt signalling pathway in α-melanocyte-stimulating hormone-treated B16F10 cells.
abstract: Antimelanogenic activity has previously been reported in ethyl acetate fraction of Ecklonia stolonifera. In this study, using the isolated dioxinodehydroeckol from the fraction, we sought to investigate an antimelanogenic signalling pathway in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells. Treatment with dioxinodehydroeckol inhibited the cellular melanin contents and expression of melanogenesis-related proteins, including microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related proteins TRP-1 and TRP-2. Moreover, dioxinodehydroeckol stimulated phosphorylation of Akt in a dose-dependent manner without affecting phosphorylation of ERK. These data suggest that dioxinodehydroeckol reduces melanin synthesis through the MITF regulation dependent upon PI3K/Akt signalling pathway.
authors.forename: Min-Sup
authors.initials: MS
authors.lastname: Lee
authors.forename: Ho-Dong
authors.initials: HD
authors.lastname: Yoon
authors.forename: Jae-Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Jae-Sue
authors.initials: JS
authors.lastname: Choi
authors.forename: Dae-Seok
authors.initials: DS
authors.lastname: Byun
authors.forename: Hyeung-Rak
authors.initials: HR
authors.lastname: Kim
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004147:Dioxins / Q000302:isolation & purification / Q000494:pharmacology*; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D051379:Mice; D000459:Phaeophyceae / Q000737:chemistry; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D000521:alpha-MSH
chemical_list: D004147:Dioxins; D008543:Melanins; C550453:dioxinodehydroeckol; D000521:alpha-MSH; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt