title: Differences in the structure and osteogenesis capacity of the periosteum from different parts of minipig mandibles.
abstract: OBJECTIVE
  To compare the structural and cellular differences of the periosteum from different parts of the mandible in minipigs by use of histologic and immunohistochemical methods to confirm the areas in which periosteal osteogenesis in situ can be used to treat mandible defects.
  
  
  METHODS
  Three minipigs were killed, and the left mandible of each was retrieved with the periosteum remaining and then fixed, decalcified, and embedded. The specimens were cut from the buccal and lingual sides of the ramus, angle, and body of the mandible and the mentum. Sections were stained with hematoxylin-eosin and antibodies for Stro-1 (stem cell marker) and vWF (endothelial cell marker). For each periosteal area, the thickness and number of positive cells for each antibody were measured and analyzed.
  
  
  RESULTS
  The mentum and mandibular angle periostea were thicker than those of the body and ramus. In addition, there were more blood vessels in the periostea of the mentum and mandibular body than the angle and ramus. There were more Stro-1-positive cells in the ramus periosteum than the mentum, body, and angle of the mandibles.
  
  
  CONCLUSIONS
  The structure and cell populations of the periosteum appear to be site specific. Therefore we suggest periosteal osteogenesis in situ to treat mentum and mandibular body defects. The periosteum should be preserved as much as possible to guarantee a good healing process.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Capital Medical University, Beijing, China.
authors.forename: Piao
authors.initials: P
authors.lastname: Wang
authors.forename: Fuwen
authors.initials: F
authors.lastname: Xie
authors.forename: Juli
authors.initials: J
authors.lastname: Pan
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Tang
mesh_terms: D053687:Adult Stem Cells / Q000737:chemistry; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D015415:Biomarkers; D002680:Chin / Q000098:blood supply / Q000502:physiology; D008334:Mandible / Q000033:anatomy & histology* / Q000098:blood supply; D009928:Organ Specificity; D010012:Osteogenesis; D010521:Periosteum / Q000033:anatomy & histology* / Q000098:blood supply / Q000502:physiology*; D017154:Stromal Cells / Q000737:chemistry; D013552:Swine; D013556:Swine, Miniature
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers