title: A novel oncogenic pathway by TLS-CHOP involving repression of MDA-7/IL-24 expression.
abstract: BACKGROUND
  Translocated in liposarcoma-CCAAT/enhancer binding protein homologous protein (TLS-CHOP) (also known as FUS-DDIT3) chimeric oncoprotein is found in the majority of human myxoid liposarcoma (MLS), but its molecular function remains unclear.
  
  
  METHODS
  We knockdowned TLS-CHOP expression in MLS-derived cell lines by a specific small interfering RNA, and analysed the gene expression profiles with microarray.
  
  
  RESULTS
  TLS-CHOP knockdown inhibited growth of MLS cells, and induced an anticancer cytokine, melanoma differentiation-associated gene 7 (MDA-7)/interleukin-24 (IL-24) expression. However, double knockdown of TLS-CHOP and MDA-7/IL-24 did not inhibit MLS cell growth.
  
  
  CONCLUSIONS
  Repression of MDA-7/IL-24 expression by TLS-CHOP is required for MLS tumour growth, and TLS-CHOP may become a promising therapeutic target for MLS treatment.
authors.affiliation: Department of Molecular Pathology, Tokyo Medical University, Shinjuku-ku, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Oikawa
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Itoh
authors.forename: M
authors.initials: M
authors.lastname: Takanashi
authors.forename: T
authors.initials: T
authors.lastname: Ozaki
authors.forename: Y
authors.initials: Y
authors.lastname: Muragaki
authors.forename: M
authors.initials: M
authors.lastname: Kuroda
mesh_terms: D015870:Gene Expression; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D018208:Liposarcoma, Myxoid / Q000235:genetics / Q000378:metabolism*; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000378:metabolism*; D034702:RNA-Binding Protein FUS / Q000235:genetics / Q000378:metabolism*; D051742:Transcription Factor CHOP / Q000235:genetics / Q000378:metabolism*; D000099180:Interleukin-24
chemical_list: D007378:Interleukins; D015514:Oncogene Proteins, Fusion; D034702:RNA-Binding Protein FUS; D051742:Transcription Factor CHOP; D000099180:Interleukin-24; C106030:TLS-CHOP fusion protein, human