title: Phyllobacterium endophyticum sp. nov., isolated from nodules of Phaseolus vulgaris.
abstract: A strain, PEPV15(T), was isolated from a nodule on Phaseolus vulgaris grown in soil in northern Spain. Phylogenetic analyses of 16S rRNA and atpD genes showed that this strain belongs to the genus Phyllobacterium. The most closely related species were, in both cases, Phyllobacterium brassicacearum, Phyllobacterium bourgognense and Phyllobacterium trifolii, the type strains of which gave sequence similarities of 98.9, 98.6 and 98.4 %, respectively, in the 16S rRNA gene and 88.1, 87.5 and 88.7 %, respectively, in the atpD gene. PEPV15(T) contained Q-10 as the major quinone (88 %) and low amounts of Q-9 (12 %). It differed from its closest relatives in its growth in diverse culture conditions and in the assimilation of several carbon sources. The strain was not able to produce nodules in Phaseolus vulgaris. The results of DNA-DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genus Phyllobacterium for which the name Phyllobacterium endophyticum sp. nov. is proposed; the type strain is PEPV15(T) ( = LMG 26470(T) = CECT 7949(T)). An emended description of the genus Phyllobacterium is also provided.
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: José-David
authors.initials: JD
authors.lastname: Flores-Félix
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Carro
authors.affiliation: Unidad Asociada Universidad de Salamanca, IRNASA (CSIC), Salamanca, Spain.|Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Encarna
authors.initials: E
authors.lastname: Velázquez
authors.affiliation: Instituto de Recursos Naturales y Agrobiología, IRNASA CSIC, Salamanca, Spain.|Unidad Asociada Universidad de Salamanca, IRNASA (CSIC), Salamanca, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Valverde
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Cerda-Castillo
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: García-Fraile
authors.affiliation: Unidad Asociada Universidad de Salamanca, IRNASA (CSIC), Salamanca, Spain.|Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Rivas
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D027805:Phaseolus / Q000382:microbiology*; D059127:Phyllobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013030:Spain
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S