title: Bioconversion of α-linolenic acid to n-3 LCPUFA and expression of PPAR-alpha, acyl Coenzyme A oxidase 1 and carnitine acyl transferase I are incremented after feeding rats with α-linolenic acid-rich oils.
abstract: High dietary intake of n-6 fatty acids in relation to n-3 fatty acids may generate health disorders, such as cardiovascular and other chronic diseases. Fish consumption rich in n-3 fatty acids is low in Latin America, it being necessary to seek other alternatives to provide α-linolenic acid (ALA), precursor of n-3 LCPUFA (EPA and DHA). Two innovative oils were assayed, chia (Salvia hispanica) and rosa mosqueta (Rosa rubiginosa). This study evaluated hepatic bioconversion of ALA to EPA and DHA, expression of PPAR-α, acyl-Coenzyme A oxidase 1 (ACOX1) and carnitine acyltransferase I (CAT-I), and accumulation of EPA and DHA in plasma and adipose tissue in Sprague-Dawley rats. Three experimental groups were fed 21 days: sunflower oil (SFO, control); chia oil (CO); rosa mosqueta oil (RMO). Fatty acid composition of total lipids and phospholipids from plasma, hepatic and adipose tissue was assessed by gas-liquid chromatography and TLC. Expression of PPAR-α (RT-PCR) and ACOX1 and CAT-I (Western blot). CO and RMO increased plasma, hepatic and adipose tissue levels of ALA, EPA and DHA and decreased n-6:n-3 ratio compared to SFO (p < 0.05, One-way ANOVA and Newman-Keuls test). CO increased levels of ALA and EPA compared to RMO (p < 0.05). No significant differences were observed for DHA levels. CO also increased the expression of PPAR-α, ACOX1 and CAT-I. Only CAT-I levels were increased by RO. CO and RMO may be a nutritional alternative to provide ALA for its bioconversion to EPA and DHA, and to increase the expression of PPAR-α, ACOX1 and CAT-I, especially CO-oil.
authors.affiliation: Oxidative Stress and Hepatotoxicity Laboratory, University of Chile, Santiago, Chile.
authors.forename: Daniel
authors.initials: D
authors.lastname: González-Mañán
authors.forename: Gladys
authors.initials: G
authors.lastname: Tapia
authors.forename: Juan Guillermo
authors.initials: JG
authors.lastname: Gormaz
authors.forename: Amanda
authors.initials: A
authors.lastname: D'Espessailles
authors.forename: Alejandra
authors.initials: A
authors.lastname: Espinosa
authors.forename: Lilia
authors.initials: L
authors.lastname: Masson
authors.forename: Patricia
authors.initials: P
authors.lastname: Varela
authors.forename: Alfonso
authors.initials: A
authors.lastname: Valenzuela
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Valenzuela
mesh_terms: D042962:Acyl-CoA Oxidase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001711:Biotransformation; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D006801:Humans; D047493:PPAR alpha / Q000235:genetics* / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027826:Rosa / Q000737:chemistry*; D027544:Salvia / Q000737:chemistry*; D015854:Up-Regulation; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D015525:Fatty Acids, Omega-3; D047493:PPAR alpha; D010938:Plant Oils; D017962:alpha-Linolenic Acid; D042962:Acyl-CoA Oxidase; D002334:Carnitine O-Palmitoyltransferase