title: Isolation of proteins from polyacrylamide gels.
abstract: Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here, we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.
authors.affiliation: Carl Gustav Carus University TU Dresden, Dresden, Germany.
authors.forename: Irene
authors.initials: I
authors.lastname: Michalk
authors.forename: Stefanie
authors.initials: S
authors.lastname: Koristka
authors.forename: Claudia
authors.initials: C
authors.lastname: Arndt
authors.forename: Michael
authors.initials: M
authors.lastname: Bachmann
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification*; D002021:Buffers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007114:Immunization / Q000379:methods; D051379:Mice; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000180:Acrylic Resins; D000941:Antigens; D002021:Buffers; D011506:Proteins; C016680:polyacrylamide gels