title: Analysis of 3-methoxypterostilbene in biological fluids by high-performance liquid chromatography: application to pre-clinical pharmacokinetics.
abstract: A method of analysis for 3-methoxypterostilbene [trans-3,3'5-trimethoxy-4'hydroxystilbene] in biological fluids is necessary to study pharmacokinetics. A novel and simple high-performance liquid chromatographic method was developed for the determination of 3-methoxypterostilbene in rat serum and urine. The internal standard, pinosylvin, was added to 0.1 mL serum or urine (serum proteins were precipitated with cold acetonitrile at -20°C). Separation was achieved with a Phenomenex® C(18) (2) (5 µm, 250 × 4.60 mm) column with ultraviolet detection at 327 nm. The calibration curves in both matrices were linear ranging from 0.05 to 100 µg/mL, and the mean extraction efficiency was >99%. Precision of the assay for both matrices was <12% (RSD) and was within 13% for all points on the calibration curve. The limit of quantification for this method was 0.05 µg/mL. The assay was successfully applied to a preliminary study of 3-methoxypterostilbene pharmacokinetics in a rat.
authors.affiliation: Faculty of Pharmacy, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Stephanie E
authors.initials: SE
authors.lastname: Martinez
authors.forename: Casey L
authors.initials: CL
authors.lastname: Sayre
authors.forename: Neal M
authors.initials: NM
authors.lastname: Davies
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine*
chemical_list: C581108:3-methoxypterostilbene; D013267:Stilbenes