title: A universal nucleoside with strong two-band switchable fluorescence and sensitivity to the environment for investigating DNA interactions.
abstract: With the aim of developing a new tool to investigate DNA interactions, a nucleoside analogue incorporating a 3-hydroxychromone (3HC) fluorophore as a nucleobase mimic was synthesized and incorporated into oligonucleotide chains. In comparison with existing fluorescent nucleoside analogues, this dye features exceptional environmental sensitivity switching between two well-resolved fluorescence bands. In labeled DNA, this nucleoside analogue does not alter the duplex conformation and exhibits a high fluorescence quantum yield. This probe is up to 50-fold brighter than 2-aminopurine, the fluorescent nucleoside standard. Moreover, the dual emission is highly sensitive to the polarity of the environment; thus, a strong shielding effect of the flanking bases from water was observed. With this nucleoside, the effect of a viral chaperone protein on DNA base stacking was site-selectively monitored.
authors.affiliation: Institut de Chimie de Nice, UMR 7272, Université de Nice Sophia Antipolis, CNRS, Parc Valrose, 06108 Nice cedex 2, France.
authors.forename: Dmytro
authors.initials: D
authors.lastname: Dziuba
authors.forename: Viktoriia Y
authors.initials: VY
authors.lastname: Postupalenko
authors.forename: Marie
authors.initials: M
authors.lastname: Spadafora
authors.forename: Andrey S
authors.initials: AS
authors.lastname: Klymchenko
authors.forename: Vincent
authors.initials: V
authors.lastname: Guérineau
authors.forename: Yves
authors.initials: Y
authors.lastname: Mély
authors.forename: Rachid
authors.initials: R
authors.lastname: Benhida
authors.forename: Alain
authors.initials: A
authors.lastname: Burger
mesh_terms: D002867:Chromones / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: C479534:3-hydroxychromone; D002867:Chromones; D005456:Fluorescent Dyes; D009705:Nucleosides; D004247:DNA