title: Cytotoxic effects of denture adhesives on primary human oral keratinocytes, fibroblasts and permanent L929 cell lines.
abstract: BACKGROUND
  To date, there have been very little data on the cytotoxic responses of different cell lines to denture adhesives.
  
  
  OBJECTIVE
  To determine the cytotoxicity of three denture adhesives on primary human oral keratinocytes (HOKs), fibroblasts (HOFs) and permanent mouse fibroblasts cell lines (L929).
  
  
  METHODS
  Three commercial denture adhesives (two creams and one powder) were prepared for indirect contact using the agar diffusion test, as well as extracts in MTT assay. The results of the MTT assay were statistically analysed by one-way anova and Tukey's test (p < 0.05).
  
  
  RESULTS
  All of the tested denture adhesives showed mild to moderate cytotoxicity to primary HOKs (p < 0.001), whereas none of three was toxic to L929 cells (p > 0.05) in both assays. For primary HOFs cultures, slight cytotoxicity was observed for one of the products from the agar diffusion test and undiluted eluates of all tested adhesives with MTT assay (p < 0.01).
  
  
  CONCLUSIONS
  Denture adhesives are toxic to the primary HOKs and HOFs cultures, whereas non-toxic to L929 cells. The results suggest that primary human oral mucosal cells may provide more valuable information in toxicity screening of denture adhesives.
authors.affiliation: Key Lab for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Fengying
authors.initials: F
authors.lastname: Chen
authors.forename: Tianfu
authors.initials: T
authors.lastname: Wu
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Cheng
mesh_terms: D000269:Adhesives / Q000633:toxicity*; D000464:Alginates / Q000633:toxicity; D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D003781:Denture Retention; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology* / Q000187:drug effects; D020723:Glucuronic Acid / Q000633:toxicity; D006603:Hexuronic Acids / Q000633:toxicity; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D008298:Maleates / Q000633:toxicity; D051379:Mice; D009061:Mouth Mucosa / Q000166:cytology* / Q000187:drug effects; D011095:Polyethylenes / Q000633:toxicity; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000269:Adhesives; D000464:Alginates; D004396:Coloring Agents; D006603:Hexuronic Acids; D008298:Maleates; D011095:Polyethylenes; D013778:Tetrazolium Salts; D013844:Thiazoles; D020723:Glucuronic Acid; C043105:poly(methyl vinyl ether-co-maleic anhydride); C022616:thiazolyl blue; D002266:Carboxymethylcellulose Sodium