title: [Distribution specificity of human fucosyltransferase 5 and its expression and localization in spermatids].
abstract: OBJECTIVE
  To investigate distribution specificity of human fucosyltransferase 5 (FUT5) as well as its expression and localization in spermatids.
  
  
  METHODS
  Human semen, vaginal swab, saliva and venous blood from healthy individuals were collected. The spermatids were isolated and the spermatid membrane protein was then extracted. Expression levels of FUT5 from human spermatid membrane, seminal plasma, vaginal fluid, saliva and serum were detected by immunoblotting technique. The expression and localization of FUT5 in spermatids were analyzed by immunofluorescent method.
  
  
  RESULTS
  Immunoblotting technique showed that FUT5 was expressed on spermatid membranes and in serum, but not in seminal plasma, vaginal fluid and saliva. The expressed FUT5 on spermatids was mostly localized on head of spermatids by fluorescent microscopy, suggesting that there was certain amount of FUT5 on human spermatid membrane, and the spermatids might be isolated from mixed stains with vaginal fluid by antigen-antibody reaction.
  
  
  CONCLUSIONS
  Human FUT5 shows a characteristic distribution specificity, and this feature may be used for identification of mixed stain involved in criminal sexual offence in future forensic practice.
authors.affiliation: Department of Forensic Serology, School of Forensic Medicine, China Medical University, Shenyang 110001, China. rui6826@yahoo.com.cn
authors.forename: Feng-Rui
authors.initials: FR
authors.lastname: Li
authors.forename: Yi-Shu
authors.initials: YS
authors.lastname: Zhou
authors.forename: Lan-Hui
authors.initials: LH
authors.lastname: Zhu
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Cui
authors.forename: Bao-Jie
authors.initials: BJ
authors.lastname: Wang
authors.forename: Mei
authors.initials: M
authors.lastname: Ding
authors.forename: Hao
authors.initials: H
authors.lastname: Pang
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods; D053803:Forensic Genetics / Q000379:methods*; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D008297:Male; D012463:Saliva / Q000378:metabolism; D012661:Semen / Q000166:cytology / Q000378:metabolism*; D013087:Spermatids / Q000378:metabolism*; D014621:Vagina / Q000378:metabolism
chemical_list: C553868:FUT5 protein, human; D005647:Fucosyltransferases