title: Identification and characterization of differentially expressed transcripts in the gills of freshwater prawn (Macrobrachium rosenbergii) under salt stress.
abstract: The giant freshwater prawn, Macrobrachium rosenbergii, is an economically important species. It is a euryhaline shrimp, surviving in wide-range salinity conditions. A change in gene expression has been suggested as an important component for stress management. To better understand the osmoregulatory mechanisms mediated by the gill, a subtractive and suppressive hybridization (SSH) tool was used to identify expressed transcripts linked to adaptations in saline water. A total of 117 transcripts represented potentially expressed under salinity conditions. BLAST analysis identified 22% as known genes, 9% as uncharacterized showing homologous to unannotated ESTs, and 69% as unknown sequences. All the identified known genes representing broad spectrum of biological pathways were particularly linked to stress tolerance including salinity tolerance. Expression analysis of 10 known genes and 7 unknown/uncharacterized genes suggested their upregulation in the gills of prawn exposed to saline water as compared to control indicating that these are likely to be associated with salinity acclimation. Rapid amplification of cDNA ends (RACE) was used for obtaining full-length cDNA of MRSW-40 clone that was highly upregulated during salt exposure. The sequenced ESTs presented here will have potential implications for future understanding about salinity acclimation and/or tolerance of the prawn.
authors.affiliation: Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture, Indian Council of Agricultural Research, Kausalyaganga, Bhubaneswar, Odisha 751002, India. hkbarman68@hotmail.com
authors.forename: Hirak Kumar
authors.initials: HK
authors.lastname: Barman
authors.forename: Swagat Kumar
authors.initials: SK
authors.lastname: Patra
authors.forename: Varsha
authors.initials: V
authors.lastname: Das
authors.forename: Shibani Dutta
authors.initials: SD
authors.lastname: Mohapatra
authors.forename: Pallipuram
authors.initials: P
authors.lastname: Jayasankar
authors.forename: Chinmayee
authors.initials: C
authors.lastname: Mohapatra
authors.forename: Ramya
authors.initials: R
authors.lastname: Mohanta
authors.forename: Rudra Prasanna
authors.initials: RP
authors.lastname: Panda
authors.forename: Surya Narayan
authors.initials: SN
authors.lastname: Rath
mesh_terms: D000818:Animals; D001483:Base Sequence; D003445:Crustacea / Q000235:genetics*; D017931:DNA Primers; D005618:Fresh Water; D005880:Gills / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012965:Sodium Chloride; D013312:Stress, Physiological
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D012965:Sodium Chloride