title: Novel durum wheat genes up-regulated in response to a combination of heat and drought stress.
abstract: We report the effect of heat, drought and combined stress on the expression of a group of genes that are up-regulated under these conditions in durum wheat (Triticum turgidum subsp. durum) plants. Modulation of gene expression was studied by cDNA-AFLP performed on RNAs extracted from flag leaves. By this approach, we identified several novel durum wheat genes whose expression is modulated under different stress conditions. We focused on a group of hitherto undescribed up-regulated genes in durum wheat, among these, 7 are up-regulated by heat, 8 by drought stress, 15 by combined heat and drought stress, 4 are up-regulated by both heat and combined stress, and 3 by both drought and combined stress. The functional characterization of these genes will provide new data that could help the developing of strategies aimed at improving durum wheat tolerance to field stress.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov. le Monteroni, 73100 Lecce, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Rampino
authors.forename: Giovanni
authors.initials: G
authors.lastname: Mita
authors.forename: Pasqua
authors.initials: P
authors.lastname: Fasano
authors.forename: Grazia Maria
authors.initials: GM
authors.lastname: Borrelli
authors.forename: Alessio
authors.initials: A
authors.lastname: Aprile
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Dalessandro
authors.forename: Luigi
authors.initials: L
authors.lastname: De Bellis
authors.forename: Carla
authors.initials: C
authors.lastname: Perrotta
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006358:Hot Temperature; D018515:Plant Leaves; D010940:Plant Proteins / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics; D014908:Triticum / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D010940:Plant Proteins