title: Rnf11-like is a novel component of NF-κB signaling, governing the posterior patterning in the zebrafish embryos.
abstract: RING finger protein 11 (RNF11) is a novel regulator of immunity and cell survival via ubiquitination process in mammalian cells whereas its vertebrate embryonic roles are undefined. Here, we are reporting the isolation, expression and functional roles of an RNF11 orthologue, Rnf11-like in zebrafish embryos. Zebrafish Rnf11-like is composed of 154 amino acids containing RING-H2-finger domain in the C-terminal region and PY-motif. Spatiotemporal expression patterns of rnf11-like indicate that rnf11-like is expressed maternally and zygotically throughout embryogenesis. However, rnf11-like transcripts are present specifically in the presomatic mesoderm (PSM), and later in the brain and retina. Knock-down of Rnf11-like using rnf11-like-specific morpholino causes cell death and developmental defects in the posterior somites, elevating transcripts of NF-κB target gene, ikk1, a negative regulator of NF-κB signaling. All these findings indicate that Rnf11-like is an essential component of NF-κB signaling pathway for specification of the posterior somites in zebrafish embryos.
authors.affiliation: Department of Biological Sciences, College of Biosciences and Biotechnology, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Sateesh
authors.initials: S
authors.lastname: Maddirevula
authors.forename: Mrudhula
authors.initials: M
authors.lastname: Anuppalle
authors.forename: Tae-Lin
authors.initials: TL
authors.lastname: Huh
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Kim
authors.forename: Myungchull
authors.initials: M
authors.lastname: Rhee
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D002352:Carrier Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins; D004625:Embryo, Nonmammalian / Q000378:metabolism; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D016328:NF-kappa B; C404508:RNF11 protein, human; D029961:Zebrafish Proteins; C574717:rnf11b protein, zebrafish