title: Quinazolinone exposure induces apoptosis and changes expressions of Fas/FasL and C-Flip in embryonic mice testicles.
abstract: Quinazolinones represent a class of sedative and anticancer drugs. Quinazolinones-based compounds have ability to suppress prostate tumor growth via apoptosis. Apoptosis is very common in embryos and adults of normal and injured mammalian testes. Effects a new derivative of quinazolinone (4(3H) quinazolinone-2-ethyl-2-phenyl ethyl (QEPE)), on the testis of Balb/C mice embryos were investigated. QEPE was able to reduce number of germ cells and diameter of seminiferous tubules. TUNEL assay analysis indicated that reduction correlated with an increase in the number of apoptotic cell. Furthermore, electron microscope observations confirmed typical apoptotic morphologies characterized by chromatin fragmentation. Finally, RT-PCR analysis showed QEPE increases the levels of Fas/Fasl and decreases C-Flip mRNAs in the testis of exposed embryos.
authors.affiliation: Developmental Biology, Animal Sciences, Faculty of Biological Sciences, Shahid-Beheshti University (SBU), G.C., Velenjak Tehran, Iran. mslahijani2006@gmail.com
authors.forename: Maryam Shams
authors.initials: MS
authors.lastname: Lahijani
authors.forename: Shirin
authors.initials: S
authors.lastname: Farivar
authors.forename: Mojgan
authors.initials: M
authors.lastname: Sarhady
authors.forename: Mitra
authors.initials: M
authors.lastname: Amiri
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001483:Base Sequence; D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000378:metabolism*; D017931:DNA Primers; D053222:Fas Ligand Protein / Q000378:metabolism*; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D052999:Quinazolinones / Q000633:toxicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000196:embryology / Q000378:metabolism*; D019014:fas Receptor
chemical_list: D053446:CASP8 and FADD-Like Apoptosis Regulating Protein; C506387:Cflar protein, mouse; D017931:DNA Primers; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D052999:Quinazolinones; D019014:fas Receptor