title: Differentiation of bone marrow-derived cells into regenerated mesothelial cells in peritoneal remodeling using a peritoneal fibrosis mouse model.
abstract: Marked thickening of the peritoneum and vasculopathy in the submesothelial compact zone have been reported in long-term peritoneal dialysis patients. Bone marrow (BM)-derived cell lines are considered to be useful tools for therapy of various diseases. To clarify the role of BM-derived cells in the peritoneal fibrosis (PF) model, we analyzed several lineages of cells in the peritoneum. BM cells from green fluorescent protein (GFP) transgenic mice were transplanted into naïve C57Bl/6 mice. Chlorhexidine gluconate (CG) was injected intraperitoneally to induce PF. Immunohistochemical analysis was performed with parietal peritoneum using anti-Sca-1 or -c-Kit and -GFP antibodies. Isolated BM cells were also transplanted into the CG-stimulated peritoneum. BM-derived cells from GFP transgenic mice appeared in the submesothelium from days 14 to 42. Both GFP- and stem cell marker-positive cells were observed in the submesothelium and on the surface. Isolated c-Kit-positive cells, transplanted into the peritoneal cavity, differentiated into mesothelial cells. In this study, we investigated whether or not BM-derived cells play a role in the repair of PF and immature cells have the potential of inducing repair of the peritoneum. The findings of this study suggest a new concept for therapy of PF.
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo, Tokyo 113-8421, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Sekiguchi
authors.forename: Chieko
authors.initials: C
authors.lastname: Hamada
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Ro
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Nakamoto
authors.forename: Masanori
authors.initials: M
authors.lastname: Inaba
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Shimaoka
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Io
authors.forename: Ichiro
authors.initials: I
authors.lastname: Koyanagi
authors.forename: Seiki
authors.initials: S
authors.lastname: Aruga
authors.forename: Jiro
authors.initials: J
authors.lastname: Inuma
authors.forename: Kayo
authors.initials: K
authors.lastname: Kaneko
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hotta
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Margetts
authors.forename: Hideki
authors.initials: H
authors.lastname: Mochizuki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Horikoshi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tomino
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D056627:Peritoneal Fibrosis / Q000378:metabolism / Q000473:pathology*; D010537:Peritoneum / Q000378:metabolism / Q000473:pathology*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism
chemical_list: D019009:Proto-Oncogene Proteins c-kit