title: [Prolonged cultivation of an anaerobic bacterial community producing hydrogen].
abstract: This paper studies various methods of long-term maintenance of the process of hydrogen evolution during the growth of an aerobic bacterial community on a starch-containing environment. When cultured in separable trip fermentation mode for 72 days, from 0.10 to 0.23 H2/l of medium/day was formed. The regime of regular reseeding lasted more than 100 days, forming an average of 0.81 1 H2/l of medium/day. The advantages and disadvantages of different methods of microbial hydrogen production during a dark starch fermentation process are presented. From the obtained H2 forming microbial communities, we isolated an anaerobic spore-forming bacterium (strain BF). Phylogenetic analysis of the 16S RNA gene sequence of the new strain showed that according to its genotype it belongs to the Clostridium butyricum species.
authors.forename: B F
authors.initials: BF
authors.lastname: Belokopytov
authors.forename: Ia V
authors.initials: IaV
authors.lastname: Ryzhmanova
authors.forename: K S
authors.initials: KS
authors.lastname: Laurinavichius
authors.forename: V A
authors.initials: VA
authors.lastname: Shcherbakova
mesh_terms: D001420:Bacteria, Aerobic / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D046972:Clostridium butyricum / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D059013:Microbial Consortia / Q000502:physiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000096:biosynthesis*; D013213:Starch / Q000378:metabolism*
chemical_list: D012336:RNA, Ribosomal, 16S; D006859:Hydrogen; D013213:Starch