title: Protein electrophoresis in agarose gels for separating high molecular weight proteins.
abstract: Very large proteins (subunit sizes >200 kDa) are difficult to electrophoretically separate on polyacrylamide gels. A SDS vertical agarose gel system has been developed that has vastly improved resolving power for very large proteins. Proteins with molecular masses between 200 and 4,000 kDa can be clearly separated. Inclusion of a reducing agent in the upper reservoir buffer has been found to be a key technical procedure for obtaining optimum resolution.
authors.affiliation: University of Wisconsin-Madison, Madison, WI, USA. mgreaser@ansci.wisc.edu
authors.forename: Marion L
authors.initials: ML
authors.lastname: Greaser
authors.forename: Chad M
authors.initials: CM
authors.lastname: Warren
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000302:isolation & purification; D003400:Astacoidea; D002021:Buffers; D004285:Dogs; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006801:Humans; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry / Q000302:isolation & purification*; D051381:Rats; D016622:Silver Staining / Q000379:methods; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D060829:Arthropod Proteins; D002021:Buffers; D009124:Muscle Proteins; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate