title: Study on the hypoglycemic activities and metabolism of alcohol extract of Alismatis Rhizoma.
abstract: The Alisma rhizoma is widely used in the therapy of diabetes in traditional folk medicine of China. Compositional analysis of the alcohol extract of Alismatis Rhizoma (AEA) revealed that the eight compounds gotten from AEA are all belonging to protostane-type triterpenes. The AEA and compounds were incubated with 3T3-L1 preadipocytes, glucose level in the 3T3-L1 adipocytes culture medium and lipid content in 3T3-L1 adipocytes were measured, and analysis of alpha-glucosidase inhibition of AEA and compounds. At the same time, the uptake of AEA by 3T3-L1 adipocytes and the metabolism of AEA in SD rats were analyzed by HPLC-ESI/MS. As result, AEA increased glucose uptake in 3T3-L1 adipocyte model, not increase adipogenesis; AEA inhibited alpha-glucosidase activity; alisol A-24-aceate (8) was absorbed by 3T3-L1 adipocytes; and two compounds were detected in blood and three were detected in urine in SD rats. So AEA had protostane-type triterpenes, these type compounds in AEA may have hypoglycemic activity via inhibition of alpha-glucosidase activity and promotion of glucose uptake. In contrast to the anti-diabetic drug thiazolidinediones, they did not induce adipogenesis, avoiding the displeased effects of rosiglitazone.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Haibin
authors.initials: H
authors.lastname: Qu
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects; D034902:Alisma / Q000737:chemistry*; D000818:Animals; D002783:Cholestenones / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome; D014315:Triterpenes / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D002783:Cholestenones; D004365:Drugs, Chinese Herbal; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D014315:Triterpenes; C457233:alisol A 24-acetate; D005947:Glucose