title: Interactions of several lipid-related gene polymorphisms and cigarette smoking on blood pressure levels.
abstract: The interactions of single nucleotide polymorphisms (SNPs) and cigarette smoking on blood pressure levels are limited. The present study was undertaken to detect nine lipid-related SNPs and their interactions with cigarette smoking on blood pressure levels. Genotyping of ATP-binding cassette transporter A1 (ABCA-1) V825I, acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) rs1044925, low density lipoprotein receptor (LDL-R) AvaⅡ, hepatic lipase gene (LIPC) -250G>A, endothelial lipase gene (LIPG) 584C>T, methylenetetrahydrofolate reductase (MTHFR) 677C>T, proprotein convertase subtilisin-like kexin type 9 (PCSK9) E670G, peroxisome proliferator-activated receptor delta (PPARD) +294T>C, and Scavenger receptor class B type 1 (SCARB1) rs5888 was performed in 935 nonsmokers and 845 smokers. The interactions were detected by factorial regression analysis. The frequencies of genotypes (ACAT-1 and LIPG), alleles (ABCA-1), and both genotypes and alleles (LDL-R, LIPC, PPARD and SCARB1) were different between nonsmokers and smokers (P < 0.05-0.001). The levels of pulse pressure (PP, ABCA-1), and systolic, diastolic blood pressure (SBP, DBP) and PP (LIPC) in nonsmokers were different among the genotypes (P < 0.01-0.001). The levels of SBP (ABCA-1, ACAT-1, LIPG and PCSK9), DBP (ACAT-1, LDL-R, LIPC, PCSK9 and PPARD), and PP (LIPC, LIPG, MTHFR and PCSK9) in smokers were different among the genotypes (P < 0.01-0.001). The SNPs of ABCA-1, ACAT-1 and PCSK9; ACAT-1, LDL-R, MTHFR and PCSK9; and ABCA-1, LIPC, PCSK9 and PPARD were shown interactions with cigarette smoking to influence SBP, DBP and PP levels (P < 0.05-0.001); respectively. The differences in blood pressure levels between the nonsmokers and smokers might partly result from different interactions of several SNPs and cigarette smoking.
authors.affiliation: Department of Cardiology, Institute of Cardiovascular Diseases, the First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, People's Republic of China. yinruixing@yahoo.com.cn
authors.forename: Rui-Xing
authors.initials: RX
authors.lastname: Yin
authors.forename: Dong-Feng
authors.initials: DF
authors.lastname: Wu
authors.forename: Jin-Zhen
authors.initials: JZ
authors.lastname: Wu
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Cao
authors.forename: Lynn Htet Htet
authors.initials: LH
authors.lastname: Aung
authors.forename: Lin
authors.initials: L
authors.lastname: Miao
authors.forename: Xing-Jiang
authors.initials: XJ
authors.lastname: Long
authors.forename: Wan-Ying
authors.initials: WY
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Meng
authors.initials: M
authors.lastname: Li
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000101:Acetyl-CoA C-Acetyltransferase / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D001794:Blood Pressure / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics; D005260:Female; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008049:Lipase / Q000235:genetics; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics; D008875:Middle Aged; D047494:PPAR delta / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D000071449:Proprotein Convertase 9; D043484:Proprotein Convertases / Q000235:genetics; D011973:Receptors, LDL / Q000235:genetics; D051122:Scavenger Receptors, Class B / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics; D012907:Smoking / Q000235:genetics*
chemical_list: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; C504173:LIPC protein, human; D047494:PPAR delta; D011973:Receptors, LDL; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2); C506639:ACAT1 protein, human; D000101:Acetyl-CoA C-Acetyltransferase; C410382:LIPG protein, human; D008049:Lipase; C472125:PCSK9 protein, human; D000071449:Proprotein Convertase 9; D043484:Proprotein Convertases; D012697:Serine Endopeptidases