title: A gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion.
abstract: The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum. Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of-function mutation in the gating of NCgl1221 leads to the oversecretion of glutamate.
authors.affiliation: Department of Biology, Tokyo Gakugei University, Koganei-shi, Tokyo, Japan. y_nakayama9339@nifty.com
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nakayama
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Iida
mesh_terms: D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D015640:Ion Channel Gating / Q000235:genetics*; D008969:Molecular Sequence Data; D020125:Mutation, Missense / Q000235:genetics*; D018408:Patch-Clamp Techniques; D013045:Species Specificity
chemical_list: D026905:Amino Acid Transport Systems; D001426:Bacterial Proteins; D018698:Glutamic Acid