title: Proteomic analysis of Taenia solium metacestode excretion-secretion proteins.
abstract: The metacestode larval stage of Taenia solium is the causal agent of a zoonotic disease called cysticercosis. The disease has an important impact on pork trade (due to porcine cysticercosis) and public health (due to human neurocysticercosis). In order to improve the current diagnostic tools and to get a better understanding of the interaction between T. solium metacestodes and their host, there is a need for more information about the proteins that are released by the parasite. In this study, we used protein sequences from different helminths, 1DE, reversed-phase LC, and MS/MS to analyze the excretion-secretion proteins produced by T. solium metacestodes from infected pigs. This is the first report of the T. solium metacestode excretion-secretion proteome. We report 76 proteins including 27 already described T. solium proteins, 17 host proteins and 32 proteins likely to be of T. solium origin, but identified using sequences from other helminths.
authors.affiliation: Veterinary Helminthology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium. bvictor@itg.be
authors.forename: Bjorn
authors.initials: B
authors.lastname: Victor
authors.forename: Kirezi
authors.initials: K
authors.lastname: Kanobana
authors.forename: Sarah
authors.initials: S
authors.lastname: Gabriël
authors.forename: Katja
authors.initials: K
authors.lastname: Polman
authors.forename: Nynke
authors.initials: N
authors.lastname: Deckers
authors.forename: Pierre
authors.initials: P
authors.lastname: Dorny
authors.forename: André M
authors.initials: AM
authors.lastname: Deelder
authors.forename: Magnus
authors.initials: M
authors.lastname: Palmblad
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003551:Cysticercosis / Q000469:parasitology / Q000662:veterinary*; D015801:Helminth Proteins / Q000032:analysis*; D013058:Mass Spectrometry; D020543:Proteome; D040901:Proteomics; D020539:Sequence Analysis, Protein; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000469:parasitology*; D041201:Taenia solium / Q000378:metabolism*
chemical_list: D015801:Helminth Proteins; D020543:Proteome