title: Polymorphic chloroplast microsatellite loci in Nelumbo (Nelumbonaceae).
abstract: OBJECTIVE
  To study population genetics, phylogeography, and hybridization of Nelumbo (Nelumbonaceae), chloroplast microsatellite markers were developed.
  
  
  RESULTS
  Seventeen microsatellite loci were identified from the chloroplast genomes of N. nucifera and N. lutea. Polymorphisms were assessed in three populations of N. nucifera and one population of N. lutea. Nine loci were found to be polymorphic in N. nucifera, and all 17 loci were found to be polymorphic in N. lutea. In N. nucifera, the number of alleles per locus ranged from two to six, and the unbiased haploid diversity per locus ranged from 0.198 to 0.790. In N. lutea, the number of alleles ranged from two to four, and the unbiased haploid diversity per locus ranged from 0.245 to 0.694.
  
  
  CONCLUSIONS
  The identified chloroplast simple sequence repeat markers will be useful for the study of genetic diversity, phylogeography, and identification of Nelumbo cultivars.
authors.affiliation: State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xue
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.forename: Shi-Liang
authors.initials: SL
authors.lastname: Zhou
mesh_terms: D000483:Alleles; D002736:Chloroplasts / Q000235:genetics*; D018742:DNA, Chloroplast / Q000737:chemistry / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005787:Gene Frequency; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D031653:Nelumbo / Q000145:classification / Q000235:genetics*; D058974:Phylogeography / Q000379:methods; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018742:DNA, Chloroplast; D018744:DNA, Plant