title: Infiltrative gastric adenocarcinoma in a chinchilla (Chinchilla lanigera).
abstract: A case of infiltrative gastric adenocarcinoma in a 5-year-old female chinchilla (Chinchilla lanigera) is described. The animal died after a 5-day period of lethargy and anorexia. Gross examination revealed increased gastric volume. The gastric wall was thickened and contained numerous firm, white nodules on the serosal surface. The lumen was obliterated by a firm mass and large amount of white, mucous secretion. Numerous white, irregular plaques and nodules were observed on the mesentery, visceral surface of the diaphragm, renal capsule, and uterine serosa (implantation metastases). Histopathological examination revealed an infiltrative gastric adenocarcinoma composed of neoplastic cells organized in a gland-like pattern interspersed with connective tissue. Neoplastic cells infiltrated the lamina propria, submucosa, and muscular layers, and were positive for cytokeratin. Detection of Helicobacter spp. in the gastric mucosa failed using Warthin-Starry and Steiner silver stains or immunohistochemistry, but a Helicobacter-specific nested polymerase chain reaction followed by sequencing was positive for Helicobacter pylori and showed more than 99% similarity to the 16S ribosomal RNA gene.
authors.affiliation: Department of Pathology, Laboratory of Veterinary Pathology, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Ricardo Barbosa
authors.initials: RB
authors.lastname: Lucena
authors.forename: Daniel Ricardo
authors.initials: DR
authors.lastname: Rissi
authors.forename: Dulciene Maria Magalhães
authors.initials: DM
authors.lastname: Queiroz
authors.forename: Claudio Severo Lombardo
authors.initials: CS
authors.lastname: Barros
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D002682:Chinchilla; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017809:Fatal Outcome; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D007150:Immunohistochemistry / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012376:Rodent Diseases / Q000382:microbiology / Q000473:pathology*; D013274:Stomach Neoplasms / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S