title: Production of vinyl derivatives from alkaline hydrolysates of corn cobs by recombinant Escherichia coli containing the phenolic acid decarboxylase from Lactobacillus plantarum CECT 748T.
abstract: The enzyme PAD from Lactobacillus plantarum CECT 748T decarboxylates some cinnamic acids namely p-coumaric acid (p-CA), caffeic acid (CA), and ferulic acid (FA) into their corresponding 4-vinyl derivatives (4-VD): 4-vinyl phenol (4-VP), 4-vinyl catechol (4-VC), and 4-vinyl guaiacol (4-VG), respectively, which are valuable food additives mainly employed as flavouring agents. The gene encoding this enzyme was cloned and overexpressed in Escherichia coli. Recombinant E. coli cells overproducing L. plantarum PAD showed a preference to degrade mainly p-CA and CA. Sterilized liquors obtained after alkaline hydrolysis of corn cob or alkaline hydrolysis of the solid residue coming from acid hydrolysis of corn cob were employed as growth media in fermentations performed in shaker or bioreactor. The fermentative process allowed converting 2222.8 mg/L p-CA into 993.9 mg/L 4-VP. The process described here allowed the production with a high-yield of a valuable food additive from a by-product of the food industry.
authors.affiliation: Laboratory of Agro-food Biotechnology, CITI-Tecnólopole, Parque Tecnológico de Galicia, San Cibrao das Viñas, Ourense, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Salgado
authors.forename: Raquel
authors.initials: R
authors.lastname: Rodríguez-Solana
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Curiel
authors.forename: Blanca
authors.initials: B
authors.lastname: de las Rivas
authors.forename: Rosario
authors.initials: R
authors.lastname: Muñoz
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Domínguez
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D000704:Analysis of Variance; D001673:Biodegradation, Environmental / Q000187:drug effects; D002262:Carboxy-Lyases / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D062385:Hydroxybenzoates / Q000302:isolation & purification / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000201:enzymology*; D011995:Recombination, Genetic / Q000187:drug effects / Q000235:genetics*; D012044:Regression Analysis; D013997:Time Factors; D014462:Ultrafiltration; D014753:Vinyl Compounds / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000468:Alkalies; D062385:Hydroxybenzoates; D014753:Vinyl Compounds; D002262:Carboxy-Lyases; C417714:phenolic acid decarboxylase; C017616:phenolic acid