title: Acetylcholinesterase inhibition, antioxidant activity and toxicity of Peumus boldus water extracts on HeLa and Caco-2 cell lines.
abstract: This work aimed to study the inhibition on acetylcholinesterase activity (AChE), the antioxidant activity and the toxicity towards Caco-2 and HeLa cells of aqueous extracts of Peumus Boldus. An IC(50) value of 0.93 mg/mL, for AChE inhibition, and EC(50) of 18.7 μg/mL, for the antioxidant activity, was determined. This activity can be attributed to glycosylated flavonoid derivatives detected, which were the main compounds, although boldine and other aporphine derivatives were also present. No changes in the chemical composition or the biochemical activities were found after gastrointestinal digestion. Toxicity of P. boldus decoction gave an IC(50) value 0.66 mg/mL for HeLa cells, which caused significant changes in the cell proteome profile.
authors.affiliation: Centro de Quíımica e Bioquímica, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: P L
authors.initials: PL
authors.lastname: Falé
authors.forename: F
authors.initials: F
authors.lastname: Amaral
authors.forename: P J
authors.initials: PJ
authors.lastname: Amorim Madeira
authors.forename: M
authors.initials: M
authors.lastname: Sousa Silva
authors.forename: M H
authors.initials: MH
authors.lastname: Florêncio
authors.forename: F N
authors.initials: FN
authors.lastname: Frazão
authors.forename: M L M
authors.initials: ML
authors.lastname: Serralheiro
mesh_terms: D000110:Acetylcholinesterase / Q000187:drug effects*; D000975:Antioxidants / Q000494:pharmacology* / Q000633:toxicity; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000494:pharmacology* / Q000633:toxicity; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006367:HeLa Cells; D006801:Humans; D031611:Peumus / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D000110:Acetylcholinesterase