title: Viscosity of supercooled aqueous glycerol solutions, validity of the Stokes-Einstein relationship, and implications for cryopreservation.
abstract: The viscosity of supercooled glycerol aqueous solutions, with glycerol mass fractions between 0.70 and 0.90, have been determined to confirm that the Avramov-Milchev equation describes very well the temperature dependence of the viscosity of the binary mixtures including the supercooled regime. On the contrary, it is shown that the free volume model of viscosity, with the parameters proposed in a recent work (He, Fowler, Toner, J. Appl. Phys. 100 (2006) 074702), overestimates the viscosity of the glycerol-rich mixtures at low temperatures by several orders of magnitude. Moreover, the free volume model for the water diffusion leads to predictions of the Stokes-Einstein product, which are incompatible with the experimental findings. We conclude that the use of these free volume models, with parameters obtained by fitting experimental data far from the supercooled and glassy regions, lead to incorrect predictions of the deterioration rates of biomolecules, overestimating their life times in these cryopreservation media.
authors.affiliation: Instituto de Química Física de los Materiales, Medio Ambiente y Energía (INQUIMAE-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, 1428 Buenos Aires, Argentina.
authors.forename: José A
authors.initials: JA
authors.lastname: Trejo González
authors.forename: M Paula
authors.initials: MP
authors.lastname: Longinotti
authors.forename: Horacio R
authors.initials: HR
authors.lastname: Corti
mesh_terms: D003080:Cold Temperature; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000737:chemistry*; D004058:Diffusion; D005990:Glycerol / Q000737:chemistry*; D008956:Models, Chemical; D012996:Solutions; D014783:Viscosity; D014867:Water / Q000737:chemistry
chemical_list: D003451:Cryoprotective Agents; D012996:Solutions; D014867:Water; D005990:Glycerol