title: The complex of cytochrome f and plastocyanin from Nostoc sp. PCC 7119 is highly dynamic.
abstract: Cytochrome f (Cyt f) and plastocyanin (Pc) form a highly transient complex as part of the photosynthetic redox chain. The complex from Nostoc sp. PCC 7119 was studied by NMR relaxation spectroscopy with the aim of determining the orientation of Pc relative to Cyt f. Chemical-shift-perturbation analysis showed that the presence of spin labels on the surface of Cyt f does not significantly affect the binding of Pc. The paramagnetic relaxation enhancement results are not consistent with a single orientation of Pc, thus indicating that multiple orientations must occur and suggesting that an encounter state represents a large fraction of the complex.
authors.affiliation: Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
authors.forename: Sandra
authors.initials: S
authors.lastname: Scanu
authors.forename: Johannes
authors.initials: J
authors.lastname: Förster
authors.forename: Michela G
authors.initials: MG
authors.lastname: Finiguerra
authors.forename: Maryam Hashemi
authors.initials: MH
authors.lastname: Shabestari
authors.forename: Martina
authors.initials: M
authors.lastname: Huber
authors.forename: Marcellus
authors.initials: M
authors.lastname: Ubbink
mesh_terms: D045348:Cytochromes f / Q000737:chemistry / Q000378:metabolism*; D008698:Mesylates / Q000378:metabolism; D008958:Models, Molecular; D046937:Nostoc; D019906:Nuclear Magnetic Resonance, Biomolecular; D010970:Plastocyanin / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: D008698:Mesylates; C074502:methanethiosulfonate; D010970:Plastocyanin; D045348:Cytochromes f