title: Pinellia ternata agglutinin produced in Bombyx mori cells exhibits bioactivity.
abstract: Pinellia ternata agglutinin (PTA) is highly homologous to many other monocot mannose-binding lectins which reportedly possess antitumor activities. Its production in silkworm cells has great application potential because the baculovirus expression system can produce post-translationally modified proteins at low cost. In the current study, the pta gene was cloned and expressed in silkworm cells, and the expressed protein was analyzed using a hemagglutination assay. A preliminary in vitro study on its anti-proliferative activity was performed. The results show that the recombinant PTA with an apparent molecular mass of 29 kDa can hemagglutinate rabbit erythrocytes and this activity can be inhibited by D-mannan at a low concentration. In addition, the recombinant hemagglutinin exhibited a dose-dependent anti-proliferative activity on hepatoma cells. The results of the current study suggest that PTA and other important bioactive proteins could be produced by silkworm bioreactor for biomedicine research and application.
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Liya
authors.initials: L
authors.lastname: Wang
authors.forename: Yaozhou
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhengbing
authors.initials: Z
authors.lastname: Lv
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D012831:Bombyx; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004912:Erythrocytes / Q000187:drug effects; D006388:Hemagglutinins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D008969:Molecular Sequence Data; D030021:Pinellia / Q000235:genetics*; D037121:Plant Lectins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D011817:Rabbits
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006388:Hemagglutinins; C047938:Pinellia ternata lectin; D037121:Plant Lectins