title: [The role of tetrapyrrol photosensitizers in photoinduced variation of free radical characteristics of rat blood in endotoxic shock].
abstract: The main goal of the present study was to evaluate the comparative effectiveness of tetrapyrrol photosensitizers (protoporphyrine IX and chlorine e6) in red (632.8 nm) and green (532.5) spectrum bands on rat blood free radical status, using the experimental model of endotoxic shock. Endotoxic shock was produced by intraperitoneal injection of lipopolysaccharide B. Irradiation effectiveness was estimated by leukocyte activation (measured with luminol-dependent chemiluminescence), superoxide dismutase activity of blood plasma (nitro blue tetrasolium assay) and lipid peroxidation (assay with cis-parinaric acid). It was found that laser irradiation has multidirectional effects on leukocyte activation, membrane lipid peroxidation and plasma SOD activity and all these effects were more pronounced in the case of endotoxic shock. Protoporphyrin was more effective in leukocyte activation and chlorine e6 demonstrated maximal effects on blood SOD activity.
authors.forename: T V
authors.initials: TV
authors.lastname: Machneva
authors.forename: A V
authors.initials: AV
authors.lastname: Lokhmatov
authors.forename: I S
authors.initials: IS
authors.lastname: Shevtsova
authors.forename: E A
authors.initials: EA
authors.lastname: Larkina
authors.forename: E P
authors.initials: EP
authors.lastname: Tkachevskaia
authors.forename: A F
authors.initials: AF
authors.lastname: Mironov
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Vladimirov
authors.forename: A N
authors.initials: AN
authors.lastname: Osipov
mesh_terms: D000818:Animals; D002735:Chlorophyllides; D005609:Free Radicals / Q000097:blood*; D007834:Lasers; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000494:pharmacology*; D011524:Protoporphyrins / Q000494:pharmacology*; D051381:Rats; D012772:Shock, Septic / Q000097:blood* / Q000139:chemically induced; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D002735:Chlorophyllides; D005609:Free Radicals; D017319:Photosensitizing Agents; D011166:Porphyrins; D011524:Protoporphyrins; C062985:phytochlorin; C028025:protoporphyrin IX; D013482:Superoxide Dismutase