title: Graphene oxide-Fe3O4 magnetic nanocomposites with peroxidase-like activity for colorimetric detection of glucose.
abstract: In this report, graphene oxide-Fe(3)O(4) magnetic nanocomposites were demonstrated to possess intrinsic peroxidase-like activity and enhanced affinity toward H(2)O(2). The nanocomposites retain their magnetic properties and can be effectively separated by a magnet. Significantly, they were proved to be novel peroxidase mimetics which could quickly catalyze oxidation of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H(2)O(2), producing a blue-colored solution. Kinetic analysis indicates that the catalytic behavior is in accord with typical Michaelis-Menten kinetics and follows a ping-pong mechanism. On the basis of the high activity, the reaction provides a simple, sensitive and selective method for colorimetric detection of glucose in diabetic urine.
authors.affiliation: National Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Ya-lei
authors.initials: YL
authors.lastname: Dong
authors.forename: Hui-ge
authors.initials: HG
authors.lastname: Zhang
authors.forename: Zia Ur
authors.initials: ZU
authors.lastname: Rahman
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.forename: Xiao-jiao
authors.initials: XJ
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.forename: Xing-guo
authors.initials: XG
authors.lastname: Chen
mesh_terms: D001560:Benzidines; D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism*; D003124:Colorimetry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D008280:Magnetics; D053761:Nanocomposites / Q000737:chemistry*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D006108:Graphite; D006861:Hydrogen Peroxide; D009195:Peroxidase; D005947:Glucose; D052203:Ferrosoferric Oxide