title: Angiotensins inhibit cell growth in GH3 lactosomatotroph pituitary tumor cell culture: a possible involvement of the p44/42 and p38 MAPK pathways.
abstract: The local renin-angiotensin system is present in the pituitary. We investigated the effects of angiotensins on GH3 lactosomatotroph cells proliferation in vitro and the involvement of p44/42 and p38 MAPK inhibitors in the growth-regulatory effects of angiotensins. Materials and Methods. Cell viability using the Mosmann method and proliferation by the measurement of BrdU incorporation during DNA synthesis were estimated. Results. Ang II and ang IV decreased the viability and proliferation of GH3 cells. Inhibitor of p44/42 MAPK attenuated the effects of ang II on cell viability and proliferation but did not affect the ang 5-8-dependent actions. Inhibitor of p38 MAPK prevented the decrease in the number of GH3 cells in ang-II- and ang-IV-treated groups. Conclusions. The growth-inhibitory effect of ang II is possibly mediated by the p44/42 MAPK. The p38 MAPK appears to mediate the inhibitory effects of both ang II and ang 5-8 upon cell survival.
authors.affiliation: Department of Endocrinology, The County Hospital of Kutno, 52 Kosciuszki Street, 99-300 Kutno, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Ptasinska-Wnuk
authors.forename: Hanna
authors.initials: H
authors.lastname: Lawnicka
authors.forename: Slawomir
authors.initials: S
authors.lastname: Mucha
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kunert-Radek
authors.forename: Marek
authors.initials: M
authors.lastname: Pawlikowski
authors.forename: Henryk
authors.initials: H
authors.lastname: Stepien
mesh_terms: D000809:Angiotensins / Q000502:physiology*; D000818:Animals; D002455:Cell Division / Q000502:physiology*; D010902:Pituitary Gland / Q000166:cytology*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats
chemical_list: D000809:Angiotensins; D011494:Protein Kinases