title: Rapid microRNA detection using power-free microfluidic chip: coaxial stacking effect enhances the sandwich hybridization.
abstract: We present a new method for rapid microRNA detection with a small volume of sample using the power-free microfluidic device driven by degassed PDMS. Target microRNA was detected by sandwich hybridization taking advantage of the coaxial stacking effect. This method allows us to detect miR-21 in 20 min with a 0.5 μL sample volume at a limit of detection of 0.62 nM. Since microRNAs can act as cancer markers, this method might substantially contribute to future point-of-care cancer diagnosis.
authors.affiliation: Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Arata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Komatsu
authors.forename: Aishan
authors.initials: A
authors.lastname: Han
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hosokawa
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D000042:Absorption; D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D046210:Microfluidic Analytical Techniques; D009693:Nucleic Acid Hybridization / Q000379:methods*; D013997:Time Factors
chemical_list: D015342:DNA Probes; D004129:Dimethylpolysiloxanes; D035683:MicroRNAs; C013830:baysilon