title: Effects of testosterone propionate on oxidative stress and the expression of spleen cytokine genes in endosulfan-treated mice.
abstract: The purpose of this research was to investigate the effects of testosterone propionate on oxidative stress and cytokine gene expression in endosulfan-treated mice. The levels of endosulfan and testosterone propionate were 0 and 0 mg x kg-1 x d(-1) (control group), 0.8 and 0 mg x kg-1 x d(-1) (endosulfan-treated group), and 0.8 and 10 mg x kg(-1) x d(-1) (experimental group), respectively. The results showed that total antioxidation capability (T-AOC) in the endosulfan-treated group was reduced significantly when compared with the control group, whereas the levels of malondialdehyde (MDA) and hydroxyl free radicals increased when compared with the control group. T-AOC levels in the experimental group were higher than that of the endosulfan-treated group, and the levels of MDA and hydroxyl free radicals decreased when compared with the endosulfan-treated group. The messenger RNA (mRNA) levels of interleukin (IL)-2 and IL-6 in the endosulfan-treated group were significantly higher than that of the control group. The mRNA levels of IL-6 in the experimental group were lower than that of the endosulfan-treated group, whereas the mRNA levels of IL-2 and interferon-γ had no significant difference between the 2 groups. The results suggest that testosterone propionate alleviates oxidative stress induced by endosulfan and at least partially reverses the changes of cytokine gene expression in mice. It is possible that androgens affect cytokine expression by alleviating oxidative stress induced by endosulfan.
authors.affiliation: Department of Laboratory Animal Science, School of Public Health and Family Medicine, Capital Medical University, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jia
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Wu
authors.forename: Xian-Qing
authors.initials: XQ
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004726:Endosulfan / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D017665:Hydroxyl Radical / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000378:metabolism*; D043343:Testosterone Propionate / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D007306:Insecticides; D007376:Interleukin-2; D015850:Interleukin-6; D012333:RNA, Messenger; D017665:Hydroxyl Radical; D008315:Malondialdehyde; D007371:Interferon-gamma; D004726:Endosulfan; D043343:Testosterone Propionate