title: Desolvation of BSA-ligand complexes measured using the quartz crystal microbalance and dual polarization interferometer.
abstract: By taking advantage of their unique difference in hydration sensitivity, we have shown that dual polarization interferometer (DPI) and quartz-crystal microbalance with dissipation monitoring (QCM-D) measurements can be used together to explore the degree of desolvation involved in the binding of small drug molecules to an immobilized bovine serum albumin film in real time. Results with DPI and QCM-D show significantly different mass values for three ligands of varying hydrophobicities that may be attributed to changes in the degree of hydration of the ligand-protein complexes in accordance with the physicochemical properties of the ligands. Furthermore, our data suggest that masses measured by QCM-D can be overwhelmed by changes in water content of ligand-protein, binary complexes, which has important consequences for future studies using mechanical resonators to study protein-binding events.
authors.affiliation: Chemistry Department, Pomona College, 645 North College Avenue, Claremont, California 91711, United States.
authors.forename: Theodore J
authors.initials: TJ
authors.lastname: Zwang
authors.forename: Reena
authors.initials: R
authors.lastname: Patel
authors.forename: Malkiat S
authors.initials: MS
authors.lastname: Johal
authors.forename: Cynthia R
authors.initials: CR
authors.lastname: Selassie
mesh_terms: D000327:Adsorption; D000818:Animals; D002110:Caffeine / Q000737:chemistry; D002417:Cattle; D003891:Desipramine / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D055767:Immobilized Proteins / Q000737:chemistry; D007368:Interferometry; D008024:Ligands; D058525:Quartz Crystal Microbalance Techniques; D020156:Salicylic Acid / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
chemical_list: D055767:Immobilized Proteins; D008024:Ligands; D012997:Solvents; D012710:Serum Albumin, Bovine; D002110:Caffeine; D020156:Salicylic Acid; D003891:Desipramine