title: Kidney safety during surgical pneumoperitoneum: an experimental study in rats.
abstract: BACKGROUND
  Elevations of intraabdominal pressure during laparoscopic procedures may lead to oliguria or anuria in mammals. Despite this, previous research has not been able to confirm an associated kidney injury. This study aimed to investigate the occurrence of an early kidney lesion secondary to surgical pneumoperitoneum in a rat model using the expression of neutrophil gelatinase-associated lipocalin (N-GAL) as a biomarker for early kidney injury.
  
  
  METHODS
  In this study, 20 male Sprague-Dawley rats under general anesthesia and mechanically ventilated were allocated to one of five experimental time-dependent groups: group 1 (1-h control), group 2 (1-h pneumoperitoneum), group 3 (2-h control), group 4 (2-h pneumoperitoneum), and group 5 (positive kidney injury group induced by intravenous administration of cisplatin 7.5 mg/kg). To evaluate the renal expression of N-GAL 24 h after the procedure, all the rats underwent a 2-h urine output evaluation as well as laparotomy and bilateral nephrectomy performed sequentially to investigate the presence of renal injury using immunofluorescence qualification and western blotting.
  
  
  RESULTS
  Urine output was reduced and N-GAL expression was increased in the animals from the cisplatin group. The animals undergoing 1- or 2-h pneumoperitoneum displayed urine output and N-GAL expression similar to that of the animals from the matching control groups.
  
  
  CONCLUSIONS
  Under the experimental conditions of this study, the animals with normal preoperative renal function did not show any type of acute kidney injury associated with the presence of a stabilized surgical pneumoperitoneum.
authors.affiliation: Division of Pediatric Surgery, University of Campinas, Campinas, SP, Brazil.
authors.forename: Rogério Fortunato
authors.initials: RF
authors.lastname: de Barros
authors.forename: Márcio Lopes
authors.initials: ML
authors.lastname: Miranda
authors.forename: Amilcar Castro
authors.initials: AC
authors.lastname: de Mattos
authors.forename: José Antonio Rocha
authors.initials: JA
authors.lastname: Gontijo
authors.forename: Vinicius Rodrigues
authors.initials: VR
authors.lastname: Silva
authors.forename: Brayner
authors.initials: B
authors.lastname: Iorio
authors.forename: Joaquim M
authors.initials: JM
authors.lastname: Bustorff-Silva
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology* / Q000378:metabolism; D000209:Acute-Phase Proteins / Q000032:analysis / Q000096:biosynthesis; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D000071068:Lipocalin-2; D054834:Lipocalins / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D014556:Urine
chemical_list: D000209:Acute-Phase Proteins; D015415:Biomarkers; C097278:Lcn2 protein, rat; D000071068:Lipocalin-2; D054834:Lipocalins; D011518:Proto-Oncogene Proteins