title: [Comparison of different magnetic beads for adsorption of pathogenic bacteria].
abstract: OBJECTIVE
  To establish a rapid enrichment method of pathogenic bacteria by comparing the adsorption capacity of the bare magnetic beads and immunomagnetic beads.
  
  
  METHODS
  With the precipitation method, the Fe3O4 magnetic beads were coated with silica and modified with the amino, which could immobilize antibody by cross-linking reaction. The adsorption capacity of different magnetic beads for pathogenic bacteria were compared.
  
  
  RESULTS
  The bare magnetic beads and Fe3O4/SiO2 immunomagnetic beads were prepared by chemical synthesis and chemical modification. There were significant differences in adsorption rates between immunomagnetic beads and magnetic beads (t = 7. 55, P < 0.05), the average adsorption rates were 96.62% and 80.89% respectively. Optimizing the washing condition, the adsorption rates of immunomagnetic beads for target and non-target bacteria were decreased from 97.03% to 93.67% and from 53.33% to 16.67% respectively. The adsorption rates of bare magnetic beads was reduced to 13.33% from 81%. No significant association was found in the adsorption rates of the immunomagnetic beads coating with single antibody and mixed antibody (P = 0.427).
  
  
  CONCLUSIONS
  The target bacteria were specifically captured by the immunomagnetic beads coationg with single antibody and mixed antibody, and it could achieve good adsorption effect based on the enrichment iptimization.
authors.affiliation: College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China. lqq_1986@163.com
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Changfei
authors.initials: C
authors.lastname: Ren
mesh_terms: D000327:Adsorption; D000907:Antibodies, Bacterial; D001419:Bacteria / Q000276:immunology / Q000302:isolation & purification*; D018189:Immunomagnetic Separation / Q000379:methods*; D012475:Salmonella / Q000276:immunology / Q000302:isolation & purification*; D012762:Shigella dysenteriae / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000302:isolation & purification*
chemical_list: D000907:Antibodies, Bacterial