title: Oxidative status and DNA damage in chidren with marasmic malnutrition.
abstract: Malnutrition as a lack of several substances containing antioxidants such as vitamins and micronutrients, while showing a predisposition for lipid peroxidation and DNA damage, is also characterized by a slowing down of the metabolic processes, which may then have protective properties against DNA damage due to a reduction in endogenous free radical production. This study aimed to examine the oxidative status and DNA damage in cases of marasmus. The study comprised 28 infants aged 6-24 months with marasmus only and 28 age-matched healthy infants. DNA damage was examined by the alkali single cell electrophoresis method (Comet assay) on mononuclear leukocytes. The total oxidant status (TOS) and total antioxidant status (TAS) were measured by colormetric auto-analyzer and the oxidative stress index (OSI) was calculated. The TOS, TAS, and OSI levels of the patient group were found to be significantly lower compared to the control group (P < 0.01, P < 0.01, P < 0.01, respectively). No statistically significant difference was found between the two groups in terms of mononuclear leukocyte DNA damage (P > 0.05). The findings of this study showed that in marasmus cases, the oxidative and antioxidative processes, which have a counteractive effect, decreased together. The other results of the study indicate that there is no increase in DNA damage in marasmus cases.
authors.affiliation: Department of Pediatrics, Children's Hospital, Sanliurfa, Turkey.
authors.forename: Maruf
authors.initials: M
authors.lastname: Celik
authors.forename: Kabil
authors.initials: K
authors.lastname: Sermatov
authors.forename: Mahmut
authors.initials: M
authors.lastname: Abuhandan
authors.forename: Dost
authors.initials: D
authors.lastname: Zeyrek
authors.forename: Abdurrahim
authors.initials: A
authors.lastname: Kocyigit
authors.forename: Akin
authors.initials: A
authors.lastname: Iscan
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D001827:Body Height; D001835:Body Weight; D016022:Case-Control Studies; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D011502:Protein-Energy Malnutrition / Q000097:blood / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D015415:Biomarkers