title: Tackling formalin-fixed, paraffin-embedded tumor tissue with next-generation sequencing.
abstract: Most tumor samples available for clinical genotyping are formalin-fixed and paraffin-embedded (FFPE), but there has been relatively little published on the suitability of such samples for next-generation sequencing approaches. A new study by Wagle and colleagues shows that a combination of hybridization-capture and deep sequencing yields high-quality data from FFPE specimens.
authors.affiliation: Department of Pathology, and Knight Cancer Institute, Oregon Health & Science University, Portland, Oregon, USA. corlessc@ohsu.edu
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Corless
authors.forename: Paul T
authors.initials: PT
authors.lastname: Spellman
mesh_terms: D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000235:genetics*