title: The ovarian cancer-derived secretory/releasing proteome: A repertoire of tumor markers.
abstract: Ovarian cancer is the most lethal gynecological malignancy worldwide, and early detection of this disease using serum or plasma biomarkers may improve its clinical outcome. In the present study, a large scale protein database derived from ovarian cancer was created to enable tumor marker discovery. First, primary organ cultures were established with the tumor tissues and corresponding normal tissues obtained from six ovarian cancer patients, and the serum-free conditioned medium (CM) samples were collected for proteomic analysis. The total proteins from the CM sample were separated by SDS-PAGE, digested with trypsin and then analyzed by LC-MS/MS. Combining data from the tumor tissues and the normal tissues, 1129 proteins were identified in total, of which those categorized as "extracellular proteins" and "plasma membrane proteins" accounted for 21.4% and 16.9%, respectively. For validation, three secretory proteins (NID1, TIMP2, and VCAN) involved in "organ development"-associated subnetwork, showed significant differences between their levels in the circulating plasma samples from ovarian cancer patients and healthy women. In conclusion, this ovarian cancer-derived protein database provides a credible repertoire of potential biomarkers in blood for this malignant disease, and deserves mining further.
authors.affiliation: State Key Laboratory of Molecular Oncology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Hongwen
authors.initials: H
authors.lastname: Yao
authors.forename: Nenan
authors.initials: N
authors.lastname: Lu
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Jia
authors.initials: J
authors.lastname: Gao
authors.forename: Suping
authors.initials: S
authors.lastname: Guo
authors.forename: Naijun
authors.initials: N
authors.lastname: Han
authors.forename: Jun
authors.initials: J
authors.lastname: Qi
authors.forename: Kaitai
authors.initials: K
authors.lastname: Zhang
authors.forename: Shujun
authors.initials: S
authors.lastname: Cheng
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wang
authors.forename: Xuemin
authors.initials: X
authors.lastname: Zhang
authors.forename: Ting
authors.initials: T
authors.lastname: Xiao
authors.forename: Lingying
authors.initials: L
authors.lastname: Wu
authors.forename: Yanning
authors.initials: Y
authors.lastname: Gao
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D030562:Databases, Protein; D005260:Female; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis / Q000097:blood; D008565:Membrane Proteins / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis* / Q000097:blood; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000032:analysis / Q000097:blood; D053675:Versicans / Q000032:analysis / Q000097:blood
chemical_list: D014408:Biomarkers, Tumor; D017077:Culture Media, Conditioned; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D020543:Proteome; C560823:TIMP2 protein, human; C508322:VCAN protein, human; C029967:nidogen; D053675:Versicans; D019716:Tissue Inhibitor of Metalloproteinase-2