title: Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization.
abstract: A novel antigen-capture sandwich ELISA system targeting the glycoproteins of the henipaviruses Nipah virus (NiV) and Hendra virus (HeV) was developed. Utilizing purified polyclonal antibodies derived from NiV glycoprotein-encoding DNA-immunized rabbits, we established a system that can detect the native antigenic structures of the henipavirus surface glycoproteins using simplified and inexpensive methods. The lowest detection limit against live viruses was achieved for NiV Bangladesh strain, 2.5 × 10(4) TCID(50). Considering the recent emergence of genetic variants of henipaviruses and the resultant problems that arise for PCR-based detection, this system could serve as an alternative rapid diagnostic and detection assay.
authors.affiliation: Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjuku, Tokyo 162-8640, Japan. ykaku@nih.go.jp
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kaku
authors.forename: Akira
authors.initials: A
authors.lastname: Noguchi
authors.forename: Glenn A
authors.initials: GA
authors.lastname: Marsh
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Barr
authors.forename: Akiko
authors.initials: A
authors.lastname: Okutani
authors.forename: Kozue
authors.initials: K
authors.lastname: Hotta
authors.forename: Boldbaatar
authors.initials: B
authors.lastname: Bazartseren
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Broder
authors.forename: Akio
authors.initials: A
authors.lastname: Yamada
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inoue
authors.forename: Lin-Fa
authors.initials: LF
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D002685:Chiroptera / Q000821:virology; D004279:DNA, Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D045404:Hendra Virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D045464:Henipavirus Infections / Q000175:diagnosis*; D008562:Membrane Glycoproteins / Q000276:immunology; D045405:Nipah Virus / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins