title: Surface plasmon resonance detection of E. coli and methicillin-resistant S. aureus using bacteriophages.
abstract: Early diagnosis and appropriate treatment of Escherichia coli (E. coli) O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) are key elements in preventing resultant life-threatening illnesses, such as hemorrhagic colitis, hemolytic uremic syndrome, and septicemia. In this report, we describe the use of surface plasmon resonance (SPR) for the biodetection of pathogenic bacteria, using bacteriophages as the recognition elements. T4 bacteriophages were used to detect E. coli, while a novel, highly specific phage was used to detect MRSA. We found that the system permits label-free, real-time, specific, rapid and cost-effective detection of pathogens, for concentrations of 10(3) colony forming units/milliliter, in less than 20 min. This system promises to become a diagnostic tool for bacteria that cause major public concern for food safety, bioterrorism, and nosocomial infections.
authors.affiliation: Regroupement Québecois de Matériaux de Pointe, Département de Génie Physique, École Polytechnique de Montreal, Case Postale 6079, succursale. Centre-Ville, Montreal, Québec, Canada H3C 3A7. nancy.tawil@polymtl.ca
authors.forename: Nancy
authors.initials: N
authors.lastname: Tawil
authors.forename: Edward
authors.initials: E
authors.lastname: Sacher
authors.forename: Rosemonde
authors.initials: R
authors.lastname: Mandeville
authors.forename: Michel
authors.initials: M
authors.lastname: Meunier
mesh_terms: D001435:Bacteriophages / Q000302:isolation & purification*; D004867:Equipment Design; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000821:virology; D019453:Escherichia coli O157 / Q000302:isolation & purification* / Q000821:virology; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification* / Q000821:virology; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000821:virology; D020349:Surface Plasmon Resonance / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors