title: Applicability of a new cell culture device for cooled-storage of stallion semen.
abstract: A new device for storage and shipping of cell cultures--the Petaka G3 cell management device--was tested for its applicability for cooled-storage of equine semen. Semen from three stallions was processed with EquiPro extender either without antibiotics (three ejaculates per stallion) or with gentamicin (250 mg/l; three ejaculates per stallion). Semen was either stored at five (anaerobic conditions) or 15 °C (aerobic conditions) in syringes or cell culture devices. Total and progressive motility, as well as membrane integrity of spermatozoa, were evaluated from days 1 to 7 after collection with computer-assisted semen analysis. In experiment 1 (extender without antibiotics), total motility, progressive motility and viability of spermatozoa significantly decreased over time (p < 0.05). The decrease was significantly faster at 15 °C than at 5 °C (p < 0.05). In the presence of gentamicin (experiment 2), this difference was no longer present. It can be concluded that cooled-storage of equine semen in sophisticated devices for cell culture is not advantageous to syringes for successful maintenance of semen longevity.
authors.affiliation: Centre for Artificial Insemination and Embryo Transfer, University for Veterinary Sciences, Vienna, Austria.
authors.forename: D
authors.initials: D
authors.lastname: Scarlet
authors.forename: S
authors.initials: S
authors.lastname: Budik
authors.forename: C
authors.initials: C
authors.lastname: Aurich
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000662:veterinary*; D006736:Horses / Q000502:physiology*; D008297:Male; D010100:Oxygen; D012034:Refrigeration / Q000662:veterinary*; D012661:Semen / Q000502:physiology; D012662:Semen Preservation / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology; D013997:Time Factors
chemical_list: D010100:Oxygen