title: A quantitative proteomic analysis of lung epithelial (A549) cells infected with 2009 pandemic influenza A virus using stable isotope labelling with amino acids in cell culture.
abstract: Influenza A virus is one of the world's major uncontrolled pathogens, causing seasonal epidemics as well as global pandemics. This was evidenced by the recent emergence and now prevalence of the 2009 swine origin pandemic H1N1 influenza A virus. In this study, quantitative proteomics using stable isotope labelling with amino acids in cell culture was used to investigate the changes in the host cell proteome in cells infected with pandemic H1N1 influenza A virus. The study was conducted in A549 cells that retain properties similar to alveolar cells. Several global pathways were affected, including cell cycle regulation and lipid metabolism, and these could be correlated with recent microarray analyses of cells infected with influenza A virus. Taken together, both quantitative proteomics and transcriptomic approaches can be used to identify potential cellular proteins whose functions in the virus life cycle could be targeted for chemotherapeutic intervention.
authors.affiliation: Centre for Emergency Preparedness and Response, Health Protection Agency, Porton Down, Salisbury, UK.
authors.forename: Brian K
authors.initials: BK
authors.lastname: Dove
authors.forename: Rebecca
authors.initials: R
authors.lastname: Surtees
authors.forename: Thomas J H
authors.initials: TJ
authors.lastname: Bean
authors.forename: Diane
authors.initials: D
authors.lastname: Munday
authors.forename: Helen M
authors.initials: HM
authors.lastname: Wise
authors.forename: Paul
authors.initials: P
authors.lastname: Digard
authors.forename: Miles W
authors.initials: MW
authors.lastname: Carroll
authors.forename: Paul
authors.initials: P
authors.lastname: Ajuh
authors.forename: John N
authors.initials: JN
authors.lastname: Barr
authors.forename: Julian A
authors.initials: JA
authors.lastname: Hiscox
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype; D007251:Influenza, Human / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D007553:Isotope Labeling; D008168:Lung / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D020543:Proteome