title: Anti-inflammatory activity of the active components from the roots of Cosmos bipinnatus in lipopolysaccharide-stimulated RAW 264.7 macrophages.
abstract: We isolated a sesquiterpene lactone from the methanol extract of the roots of Cosmos bipinnatus, namely, MDI (a mixture of dihydrocallitrisin and isohelenin). The anti-inflammatory activity of MDI was evaluated using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. MDI significantly inhibited the expression of inducible nitric oxide synthase and cyclooxygenase-2. Consistent with these results, the production of NO and prostaglandin E2 (PGE2) was suggested to be suppressed by MDI in a concentration-dependent manner (IC50 value was 0.94 and 2.88 µg mL(-1) for NO and PGE2, respectively). In addition, MDI significantly inhibited the expressions of pro-inflammatory cytokines such as IL-1β, IL-6, IFN-γ and TNF-α. Furthermore, MDI attenuated DNA-binding activity of NF-κB by inhibiting the phosphorylation of IκB. These results indicate that MDI isolated from the roots of C. bipinnatus shows anti-inflammatory activity in LPS-stimulated murine macrophages by modulating the NF-κB pathway.
authors.affiliation: Department of Life Science, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Sohn
authors.forename: Bong-Sik
authors.initials: BS
authors.lastname: Yun
authors.forename: So-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Wahn-Soo
authors.initials: WS
authors.lastname: Choi
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Jeon
authors.forename: Jun-Sik
authors.initials: JS
authors.lastname: Yoo
authors.forename: Si-Kwan
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002460:Cell Line; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides