title: Ammonium is toxic for aging yeast cells, inducing death and shortening of the chronological lifespan.
abstract: Here we show that in aging Saccharomyces cerevisiae (budding yeast) cells, NH(4) (+) induces cell death associated with shortening of chronological life span. This effect is positively correlated with the concentration of NH(4) (+) added to the culture medium and is particularly evident when cells are starved for auxotrophy-complementing amino acids. NH(4) (+)-induced cell death is accompanied by an initial small increase of apoptotic cells followed by extensive necrosis. Autophagy is inhibited by NH(4) (+), but this does not cause a decrease in cell viability. We propose that the toxic effects of NH(4) (+) are mediated by activation of PKA and TOR and inhibition of Sch9p. Our data show that NH(4) (+) induces cell death in aging cultures through the regulation of evolutionary conserved pathways. They may also provide new insights into longevity regulation in multicellular organisms and increase our understanding of human disorders such as hyperammonemia as well as effects of amino acid deprivation employed as a therapeutic strategy.
authors.affiliation: Life and Health Sciences Research Institute, ICVS, School of Health Sciences, University of Minho, Braga, Portugal.
authors.forename: Júlia
authors.initials: J
authors.lastname: Santos
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Sousa
authors.forename: Cecília
authors.initials: C
authors.lastname: Leão
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects* / Q000502:physiology; D016922:Cellular Senescence / Q000187:drug effects* / Q000502:physiology; D003470:Culture Media; D000644:Quaternary Ammonium Compounds / Q000009:adverse effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology; D015003:Yeasts / Q000187:drug effects* / Q000502:physiology
chemical_list: D000596:Amino Acids; D003470:Culture Media; D000644:Quaternary Ammonium Compounds