title: Antiangiogenic effects of p-coumaric acid in human endothelial cells.
abstract: p-Coumaric acid, a hydroxy derivative of cinnamic acid, has been known to possess antioxidant and anticancer activities. Despite its potential contribution to chemopreventive effects, the mechanism by which p-coumaric acid exerts its antiangiogenic actions remains elusive. In this study, we revealed that p-coumaric acid inhibited the sprouting of endothelial cells in rat aortic rings and inhibited the tube formation and migration of endothelial cells. We observed that p-coumaric acid could downregulate mRNA expression levels of the key angiogenic factors vascular endothelial growth factor and basic fibroblast growth factor. Also, we demonstrated that p-coumaric acid inhibited both the AKT and ERK signaling pathways, which are known to be crucial for angiogenesis. Using a mouse model, we also showed that p-coumaric acid effectively suppressed tumor growth in vivo by lowering hemoglobin contents. Collectively, these findings indicate that p-coumaric acid possesses potent anticancer properties due to the inhibition of angiogenesis in vivo.
authors.affiliation: Department of Biomedical Science, Biomedical Science Institute, School of Medicine, Kyung Hee University, Seoul, 130-701, Korea.
authors.forename: Chang-Seok
authors.initials: CS
authors.lastname: Kong
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Jeong
authors.forename: Jae-Sun
authors.initials: JS
authors.lastname: Choi
authors.forename: Kil-Jung
authors.initials: KJ
authors.lastname: Kim
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Jeong
mesh_terms: D000230:Adenocarcinoma / Q000098:blood supply; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001011:Aorta / Q000166:cytology; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003373:Coumaric Acids / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D011422:Propionates; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents; D003373:Coumaric Acids; D011422:Propionates; D017382:Reactive Oxygen Species; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; C495469:p-coumaric acid