title: Indole-3-acetic acid biosensor based on G-rich DNA labeled AuNPs as chemiluminescence probe coupling the DNA signal amplification.
abstract: A highly sensitive chemiluminescence (CL) method for detection of phytohormone indole-3-acetic acid (IAA) was developed by using G-rich DNA labeled gold nanoparticles (AuNPs) as CL probe coupling the DNA signal amplification technology. The IAA antibody was immobilized on carboxyl terminated magnetic beads (MBs). In the presence of IAA, antibody labeled AuNPs were captured by antibody functionalized MBs. The DNA on AuNPs is released by a ligand exchange process induced by the addition of DTT. The released DNA is then acted as the linker and hybridized with the capture DNA on MBs and probe DNA on AuNPs CL probe. The CL signal is obtained via the instantaneous derivatization reaction between a specific CL reagent, 3,4,5-trimethoxyl-phenylglyoxal (TMPG), and the G-rich DNA on AuNPs CL probe. IAA can be detected in the concentration range from 0.02 ng/mL to 30 ng/mL, and the limit of detection is 0.01 ng/mL.
authors.affiliation: Key Laboratory of Eco-chemical Engineering, Qingdao 266042, China. hunxu@sohu.com
authors.forename: Xu
authors.initials: X
authors.lastname: Hun
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Mei
authors.forename: Zhouping
authors.initials: Z
authors.lastname: Wang
authors.forename: Yunhua
authors.initials: Y
authors.lastname: He
mesh_terms: D000040:Abscisic Acid / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D003583:Cytokinins / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry; D018525:Germination; D005875:Gibberellins / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D007210:Indoleacetic Acids / Q000032:analysis*; D049449:Luminescence; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D008863:Microspheres; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012815:Signal Processing, Computer-Assisted; D013194:Staining and Labeling
chemical_list: D003583:Cytokinins; D005875:Gibberellins; D007210:Indoleacetic Acids; D010936:Plant Extracts; D006147:Guanine; C030737:indoleacetic acid; D000040:Abscisic Acid; D006046:Gold; D004247:DNA; C007842:gibberellic acid