title: Detection on dynamic changes of endothelial cell specific molecule-1 in acute rejection after renal transplantation.
abstract: OBJECTIVE
  To investigate the value of dynamic monitoring of endothelial cell specific molecule-1 (ESM-1) in diagnosing acute rejection after renal transplantation.
  
  
  METHODS
  ESM-1 expression was observed in peripheral blood circulating endothelial cells and renal allografts after renal transplantation, and was compared to the flow cytometry (FCM) results of urine HLA-DR(+) lymphocytes.
  
  
  RESULTS
  In patients with acute rejection, ESM-1 mRNA and protein expression increased significantly (P < .01). Real-time polymerase chain reaction detection of ESM-1 mRNA in peripheral blood cells was more sensitive and specific than FCM detection of urine HLA-DR(+) lymphocytes. Moreover, real-time PCR detection is characterized by convenient sampling and good reproducibility.
  
  
  CONCLUSIONS
  ESM-1 is a new marker for endothelial cell activation. However, its significance during acute rejection in renal transplantation is still unclear. Our study demonstrates that ESM-1 may reflect the degree of endothelial cell injury in renal allografts, and that it may serve as a highly sensitive and specific marker for acute rejection after renal transplantation.
authors.affiliation: Department of Urology, General Hospital of Chengdu Military Command, Chengdu, China. lishadan@hotmail.com
authors.forename: Shadan
authors.initials: S
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Qingtang
authors.initials: Q
authors.lastname: Wang
authors.forename: Hang
authors.initials: H
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Liang
authors.forename: Fengshuo
authors.initials: F
authors.lastname: Jin
mesh_terms: D000208:Acute Disease; D000328:Adult; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D009363:Neoplasm Proteins / Q000502:physiology*; D011509:Proteoglycans / Q000502:physiology*
chemical_list: C101134:ESM1 protein, human; D009363:Neoplasm Proteins; D011509:Proteoglycans