title: RGDS-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by HeLa cells.
abstract: The objective of this study was to develop thin, biocompatible, and biofunctional hydrogel-coated small-sized nanoparticles that exhibit favorable stability, viability, and specific cellular uptake. This article reports the coating of magnetic iron oxide nanoparticles (MIONPs) with covalently cross-linked biofunctional polyethylene glycol (PEG) hydrogel. Silanized MIONPs were derivatized with eosin Y, and the covalently cross-linked biofunctional PEG hydrogel coating was achieved via surface-initiated photopolymerization of PEG diacrylate in aqueous solution. The thickness of the PEG hydrogel coating, between 23 and 126 nm, was tuned with laser exposure time. PEG hydrogel-coated MIONPs were further functionalized with the fibronectin-derived arginine-glycine-aspartic acid-serine (RGDS) sequence, in order to achieve a biofunctional PEG hydrogel layer around the nanoparticles. RGDS-bound PEG hydrogel-coated MIONPs showed a 17-fold higher uptake by the human cervical cancer HeLa cell line than that of amine-coated MIONPs. This novel method allows for the coating of MIONPs with nano-thin biofunctional hydrogel layers that may prevent undesirable cell and protein adhesion and may allow for cellular uptake in target tissues in a specific manner. These findings indicate that the further biofunctional PEG hydrogel coating of MIONPs is a promising platform for enhanced specific cell targeting in biomedical imaging and cancer therapy.
authors.affiliation: Graduate School of Sciences and Engineering, Koç University, Istanbul, Turkey.
authors.forename: Caner
authors.initials: C
authors.lastname: Nazli
authors.forename: Tugba Ipek
authors.initials: TI
authors.lastname: Ergenc
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Yar
authors.forename: Havva Yagci
authors.initials: HY
authors.lastname: Acar
authors.forename: Seda
authors.initials: S
authors.lastname: Kizilel
mesh_terms: D001693:Biological Transport, Active; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D020100:Hydrogels; D007501:Iron / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D050997:Nanomedicine; D009842:Oligopeptides / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D058185:Magnetite Nanoparticles; D009842:Oligopeptides; D011092:Polyethylene Glycols; C046617:arginyl-glycyl-aspartyl-serine; D007501:Iron