title: Development of macrolide lactone antibiotic brefeldin A fermentation process with Eupenicillium brefeldianum ZJB082702.
abstract: In this work, a robust brefeldin A-synthesizing fungus, Eupenicillium brefeldianum ZJB082702, was bred from a Murraya paniculata endophytic fungus E. brefeldianum A1163. Using one-factor-at-a-time experimental design, optimization of media composition for E. brefeldianum ZJB082702 fermenting brefeldin A was conducted. Outcomes indicated that mixed carbon source and mixed nitrogen source were of c ritical importance to brefeldin A fermentation. After 6d culture in the optimized fermentation media, composed of (gl(-1)) 13.33 starch, 26.67 glucose, 1.0 yeast extract powder, 1.0 corn steep liquor, 0.5 soybean meal, 0.75 NaNO(3), 2.5 malt extract, 6.0 CaCO(3), 3.0 MgSO(4), 4.0 KH(2)PO(4), 1.0 × 10(-2) CuSO(4), brefeldin A yield peaked at 1304.7 mgl(-1), 648.2 mgl(-1) in 500 ml baffled flask and 15 l stirred fermentor respectively, formed as a growth associated type of secondary metabolite based on fermentation profile analysis.
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Ye-Fei
authors.initials: YF
authors.lastname: Wu
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Xue
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D019149:Bioreactors; D020126:Brefeldin A / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D055324:Eupenicillium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D009584:Nitrogen / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D020126:Brefeldin A; D002244:Carbon; D009584:Nitrogen