title: Origin of non-cardiac endothelial cells from an Isl1+ lineage.
abstract: The cardiovascular system consists of many cell types with distinct embryonic origins. Cells from an Islet1 (Isl1)-expressing progenitor population make a substantial contribution to the developing heart. We reasoned that cells derived from Isl1-expressing progenitors might contribute more widely to the cardiovascular system. We show that cells derived from an Isl1-expressing progenitor lineage make a wide contribution to the systemic vasculature and that embryos conditionally deficient for Rac1 within this cell population develop defects in the non-cardiac vasculature. These data define new roles for Isl1 in the developing embryo and demonstrate a contribution of Isl1-expressing progenitors to vascular endothelium in vivo.
authors.affiliation: Institute of Genetic Medicine, Newcastle University, Newcastle Upon Tyne NE1 3BZ, UK.
authors.forename: Iain D
authors.initials: ID
authors.lastname: Keenan
authors.forename: Hong Jun
authors.initials: HJ
authors.lastname: Rhee
authors.forename: Bill
authors.initials: B
authors.lastname: Chaudhry
authors.forename: Deborah J
authors.initials: DJ
authors.lastname: Henderson
mesh_terms: D000818:Animals; D002319:Cardiovascular System; D002454:Cell Differentiation; D019070:Cell Lineage; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology; D060850:LIM-Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D009206:Myocardium / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020830:rac1 GTP-Binding Protein
chemical_list: D060850:LIM-Homeodomain Proteins; D009479:Neuropeptides; C484602:Rac1 protein, mouse; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1; D020744:rac GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein