title: Cell proliferation and apoptosis in prostate needle biopsies with adenocarcinoma Gleason score 6 or 7.
abstract: OBJECTIVE
  To analyze whether cell proliferation and apoptotic indexes in needle biopsies with prostate cancer Gleason 6 or 7 can identify more objectively Gleason score 6 or 7 in needle biopsy samples.
  
  
  METHODS
  Seventy patients diagnosed by needle biopsy and treated by radical prostatectomy were included. Fifty cases were Gleason score 6 and 20 were Gleason 7. Twenty-two cases were organ-confined and 48 nonorgan-confined. Histologic sections from needle biopsies were stained for cell proliferation using the MIB 1 (Ki67) antibody and in situ end-labeling technique for apoptosis and recorded as the percentage of positive cells. Statistical analysis included Student's t-test, Pearson's test, and logistic regression analysis.
  
  
  RESULTS
  We found increased apoptotic and proliferation indexes from Gleason 6 to 7, and from organ-confined to non-organ-confined. Apoptotic index was not associated to stage or Gleason score. We identified an association between cell proliferation and Gleason score indicating that higher proliferation index is associated with a higher probability of presenting a Gleason score 7. There was no association between pathologic stage and cell proliferation.
  
  
  CONCLUSIONS
  Cell proliferation and apoptosis can distinguish a subset of aggressive lesions in needle biopsies with Gleason 6 or 7 prostate adenocarcinoma.
authors.affiliation: Unit of Anatomic Pathology, Department of Surgery, Faculty of Medicine, Cordoba University Medical School, Cordoba, Spain. em1lobea@uco.es
authors.forename: Antonio
authors.initials: A
authors.lastname: Lopez-Beltran
authors.forename: Liang
authors.initials: L
authors.lastname: Cheng
authors.forename: Ana
authors.initials: A
authors.lastname: Blanca
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Montironi
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D017209:Apoptosis; D001707:Biopsy, Needle; D002455:Cell Division; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading / Q000379:methods*; D011467:Prostate / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D019394:Ki-67 Antigen