title: [Analysis of 20 sulfonamides in sewage sludge by high performance liquid chromatography tandem mass spectrometry].
abstract: OBJECTIVE
  To develop a comprehensive method for simultaneous analysis of 20 sulfonamides in sewage sludge by liquid chromatography tandem mass spectrometry (LC-MS/MS).
  
  
  METHODS
  Ultrasonic extraction and accelerated solvent extraction was compared with respect to the recovery of target drugs from sludge. Cleanup procedure was optimized during the test. The sludge samples were ultrasonicly extracted and purified by a mix mode anion-ion exchange solid-phase extraction, following determination using LC-MS/MS. 20 sulfonamides were separated by a C18 column and analyzed under positive ion mode with multi-reaction monitoring. Isotopic internal standards calibration was used for quantification.
  
  
  RESULTS
  The method quantification limits for 20 analytes were 0.25-10.0 microg/kg with overall recoveries of 50.1%-120.0%, and the relative standard deviations less than 30%. The method was finally used to analyze sulfonamides in sludge from sewage treatment plants in Beijing, and 3 target compounds (sulfadiazine, sulfapyridine and sulfamethoxazole) were detected at a concentration range of 2.1-153.1 microg/kg.
  
  
  CONCLUSIONS
  This method could be applied in simultaneous analysis of 20 sulfonamides in municipal sludge samples.
authors.affiliation: School of Public Health and Family Medicine, Capital Medical University, Beijing 100069, China. serenashuo@hotmail.com
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Shao
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D002947:Cities; D004350:Drug Residues / Q000032:analysis*; D012722:Sewage / Q000737:chemistry*; D013449:Sulfonamides / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D012722:Sewage; D013449:Sulfonamides