title: Use of a blocking primer allows selective amplification of bacterial DNA from microalgae cultures.
abstract: The production of algae is a crucial component of many aquaculture systems and the role of bacteria in this process is an important although complex one. We report the development of a new blocking primer that allowed PCR amplification of bacterial DNA in the presence of algal chloroplast DNA.
authors.affiliation: Australian Seafood Cooperative Research Centre and the Tasmanian Institute of Agriculture, University of Tasmania, Hobart, Tasmania, Australia. shane.powell@utas.edu.au
authors.forename: Shane M
authors.initials: SM
authors.lastname: Powell
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Chapman
authors.forename: Michel
authors.initials: M
authors.lastname: Bermudes
authors.forename: Mark L
authors.initials: ML
authors.lastname: Tamplin
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics; D017756:Aquaculture; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D041965:Flavobacteriaceae / Q000235:genetics; D058086:Microalgae / Q000382:microbiology*; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S