title: AtPP2CG1, a protein phosphatase 2C, positively regulates salt tolerance of Arabidopsis in abscisic acid-dependent manner.
abstract: AtPP2CG1 (Arabidopsis thaliana protein phosphatase 2C G Group 1) was predicted as an abiotic stress candidate gene by bioinformatic analysis in our previous study. The gene encodes a putative protein phosphatase 2C that belongs to Group G of PP2C. There is no report of Group G genes involved in abiotic stress so far. Real-time RT-PCR analysis showed that AtPP2CG1 expression was induced by salt, drought, and abscisic acid (ABA) treatment. The expression levels of AtPP2CG1 in the ABA synthesis-deficient mutant abi2-3 were much lower than that in WT plants under salt stress suggesting that the expression of AtPP2CG1 acts in an ABA-dependent manner. Over-expression of AtPP2CG1 led to enhanced salt tolerance, whereas its loss of function caused decreased salt tolerance. These results indicate that AtPP2CG1 positively regulates salt stress in an ABA-dependent manner. Under salt treatment, AtPP2CG1 up-regulated the expression levels of stress-responsive genes, including RD29A, RD29B, DREB2A and KIN1. GUS activity was detected in roots, leaves, stems, flower, and trichomes of AtPP2CG1 promoter-GUS transgenic plants. AtPP2CG1 protein was localized in nucleus and cytoplasm via AtPP2CG1:eGFP and YFP:AtPP2CG1 fusion approaches.
authors.affiliation: Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin 150030, PR China. fangfei6073@126.com
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Zhai
authors.forename: Hua
authors.initials: H
authors.lastname: Cai
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Xiao
authors.initials: X
authors.lastname: Luo
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Xi
authors.initials: X
authors.lastname: Bai
mesh_terms: D000040:Abscisic Acid / Q000502:physiology*; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D018506:Gene Expression Regulation, Plant; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified; D000071636:Protein Phosphatase 2C; D055049:Salt Tolerance / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D000040:Abscisic Acid; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C