title: Characterization of microsatellite loci from Litsea hypophaea (Lauraceae), a tree endemic to Taiwan.
abstract: OBJECTIVE
  Microsatellite primers were developed for the endemic tree Litsea hypophaea (Lauraceae) in Taiwan to investigate its genetic diversity and population genetic structure and to investigate species delimitation within Litsea.
  
  
  RESULTS
  Fifteen new simple sequence repeat markers were developed from L. hypophaea with a magnetic bead enrichment method. Most loci were also amplified from three closely related species, L. coreana, L. lii, and L. acutivena. The number of alleles and observed and expected heterozygosities across loci varied with a range of 1-25, 0.000-1.000, and 0.000-0.956, respectively.
  
  
  CONCLUSIONS
  The application of these microsatellite markers of L. hypophaea provides a tool for understanding genetic diversity and population differentiation. In addition, interspecific amplification suggests that these markers will also be useful for species identification of related taxa within Litsea in Taiwan.
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors.forename: Yu-Chung
authors.initials: YC
authors.lastname: Chiang
authors.forename: Huei-Chuan
authors.initials: HC
authors.lastname: Shih
authors.forename: Min-Chun
authors.initials: MC
authors.lastname: Huang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ju
authors.forename: Kuo-Hsiang
authors.initials: KH
authors.lastname: Hung
mesh_terms: D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D032862:Litsea / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013624:Taiwan; D014197:Trees / Q000145:classification / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant