title: SC1/hevin identifies early white matter injury after ischemia and intracerebral hemorrhage in young and aged rats.
abstract: The progression of white matter damage after ischemic and hemorrhagic strokes can exacerbate the initial injury, but little is known about the processes involved. We show that the antiadhesive matricellular glycoprotein SC1 is a novel early marker of white matter damage in 3 models of acute injury in the rat striatum: transient focal ischemia, intracerebral hemorrhage, and a needle penetration wound. SC1 was restricted to the damaged portions of axon bundles that bordered stroke lesions in young-adult and aged rats. SC1 peaked at 1 and 3 days after intracerebral hemorrhage and at 7 days after ischemia. The SC1-positive bundles usually expressed degraded myelin basic protein and amyloid precursor protein, a marker of axonal injury. At the hematoma edge, SC1 was seen in a few axon bundles that retained myelin basic protein staining. In these bundles, punctate SC1 staining filled individual axons, extended beyond a core of pan-axonal neurofilament and NF200 and was inside or overlapped with myelin basic protein staining when it was present. Aged rats had less SC1 (and amyloid precursor protein) after both types of stroke, suggesting a reduced axonal response. SC1 also labeled amyloid precursor protein-positive axon bundles along the needle penetration tract of saline-injected rats; thus, SC1 appears to characterize damaged striatal white matter damage after multiple types of injury.
authors.affiliation: Toronto Western Research Institute, University Health Network, Toronto, Ontario, Canada.
authors.forename: Starlee
authors.initials: S
authors.lastname: Lively
authors.forename: Lyanne C
authors.initials: LC
authors.lastname: Schlichter
mesh_terms: D000375:Aging / Q000502:physiology; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000473:pathology; D015415:Biomarkers; D001921:Brain / Q000473:pathology*; D002545:Brain Ischemia / Q000473:pathology*; D002135:Calcium-Binding Proteins / Q000502:physiology*; D002543:Cerebral Hemorrhage / Q000473:pathology*; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D007839:Functional Laterality; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism / Q000473:pathology; D008297:Male; D018613:Microscopy, Confocal; D009413:Nerve Fibers, Myelinated / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000473:pathology*; D015854:Up-Regulation / Q000502:physiology; D014951:Wounds, Stab / Q000473:pathology
chemical_list: D016564:Amyloid beta-Protein Precursor; D015415:Biomarkers; D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; C503128:Sparcl1 protein, rat