title: Specific targeting of human interleukin (IL)-13 receptor α2-positive cells with lentiviral vectors displaying IL-13.
abstract: The ability to selectively and efficiently target transgene delivery to specific cell types in vitro and in vivo remains one of the formidable challenges in gene therapy. Lentiviral vectors have several advantages that make them attractive as gene delivery vehicles and their tropism can be altered through pseudotyping, allowing transgene delivery to specific populations of cells. The human interleukin-13 receptor α2 (IL-13Rα2) is uniquely overexpressed in many different human tumors, making it an attractive target for cancer therapy. In this study, we examined whether IL-13Rα2-positive tumor cells can be specifically targeted with lentiviral vector pseudotypes containing a truncated fusion (F) protein derived from measles virus (MV) and a tail-truncated and receptor-blind MV hemagglutinin (H) protein bearing IL-13 at the C terminus. The retargeted lentiviral vector efficiently transduced cells that express high levels of IL-13Rα2, but not cells expressing low levels of IL-13Rα2 in vitro. In vivo, it specifically targeted IL-13Rα2-positive glioma cell xenografts in immunodeficient mice in the context of subcutaneous and intracranial glioma models. Similar lentiviral vectors may be developed for targeting other tumors expressing specific cell surface receptors.
authors.affiliation: Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD 20892, USA.
authors.forename: Wu
authors.initials: W
authors.lastname: Ou
authors.forename: Michael P
authors.initials: MP
authors.lastname: Marino
authors.forename: Akiko
authors.initials: A
authors.lastname: Suzuki
authors.forename: Bharat
authors.initials: B
authors.lastname: Joshi
authors.forename: Syed R
authors.initials: SR
authors.lastname: Husain
authors.forename: Andrea
authors.initials: A
authors.lastname: Maisner
authors.forename: Evanthia
authors.initials: E
authors.lastname: Galanis
authors.forename: Raj K
authors.initials: RK
authors.lastname: Puri
authors.forename: Jakob
authors.initials: J
authors.lastname: Reiser
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D018793:Interleukin-13 / Q000235:genetics*; D053663:Interleukin-13 Receptor alpha2 Subunit / Q000378:metabolism*; D016086:Lentivirus / Q000235:genetics*; D051379:Mice
chemical_list: D018793:Interleukin-13; D053663:Interleukin-13 Receptor alpha2 Subunit