title: [The sources of inorganic sulfur in the process of cluster protein Fnr[4Fe-4S]2+ reconstruction in Escherichia coli cells cultivated with NO-donating agents].
abstract: Dinitrosyl iron complexes (DNICs) with thiol ligands--binuclear and mononuclear--inhibited aidB gene expression in E. coli cells. This process is due to the nitrosylation of the active center in iron-sulfur protein Fnr [4Fe-4S]2+ by low-molecular DNICs. The next step is transformation of the above DNICs into the DNICs with the thiol groups in the apo-form of Fnr protein. These nitrosylated proteins are characterized by the EPR signal with g perpendicular = 2.04 and g parallel 1 = 2,014. An addition of sulfur containing L-Cys or N-A-L-Cys as well as Na2S to the cells lead to the increasing in the aidB gene expression simultaneously with an appearance of the EPR signal with g perpendicular = 2.04 and g parallel = 2.02 as the characteristics of the DNICs with persulfide (R-S-S-) ligands. We suppose that the recovery of the aidB gene activity was due to the accumulation of inorganic sulfur in the cells and reconstruction of the active center in Fnr[4Fe-4S]2+. It appears that the above process is the function of L-cysteine-desulfurase protein which repaired the active center of Fnr[4Fe-4S]2+ protein using the sulfur from L-Cys or N-A-L-Cys after its deacetylation. On the other side the ions of inorganic sulfur being reacted with SH-groups led to the transformation of DNIC with thiol ligands into the persulfides. Na2S was the most potent activator of the aidB gene expression in our experiments.
authors.forename: S V
authors.initials: SV
authors.lastname: Vasil'eva
authors.forename: D A
authors.initials: DA
authors.lastname: Strel'tsova
authors.forename: A V
authors.initials: AV
authors.lastname: Vlaskina
authors.forename: V D
authors.initials: VD
authors.lastname: Mikoian
authors.forename: A F
authors.initials: AF
authors.lastname: Vanin
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology; D007501:Iron / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009589:Nitrogen Oxides / Q000378:metabolism; D013440:Sulfides / Q000378:metabolism* / Q000494:pharmacology; D013455:Sulfur / Q000378:metabolism / Q000494:pharmacology
chemical_list: C090096:AidB protein, E coli; D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D007506:Iron-Sulfur Proteins; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; D013440:Sulfides; C073661:dinitrosyl iron complex; D013455:Sulfur; D007501:Iron; C033479:sodium sulfide