title: Increase in local protein concentration by field-inversion gel electrophoresis.
abstract: Proteins that migrate through cross-linked polyacrylamide gels (PAGs) under the influence of a constant electric field experience negative factors, such as diffusion and nonspecific trapping in the gel matrix. These negative factors reduce protein concentrations within a defined gel volume with increasing migration distance and, therefore, decrease protein recovery efficiency. Here, we describe the enhancement of protein separation efficiency up to twofold in conventional one-dimensional PAG electrophoresis (1D PAGE), two-dimensional (2D) PAGE, and native PAGE by implementing pulses of inverted electric field during gel electrophoresis.
authors.affiliation: Genome institute of Singapore, Singapore, Singapore.
authors.forename: Henghang
authors.initials: H
authors.lastname: Tsai
authors.forename: Hon-Chiu Eastwood
authors.initials: HC
authors.lastname: Leung
mesh_terms: D000818:Animals; D002021:Buffers; D003720:Densitometry; D016521:Electrophoresis, Gel, Pulsed-Field / Q000295:instrumentation / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry; D008970:Molecular Weight; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D051381:Rats
chemical_list: D002021:Buffers; D011506:Proteins