title: Down-regulation of the epidermal growth factor receptor by altering N-glycosylation: emerging role of β1,4-galactosyltransferases.
abstract: OBJECTIVE
  Blocking N-glycosylation of the epidermal growth factor receptor (EGFR) by tunicamycin inhibits its cellular accumulation. Due to the toxic potential of this drug, finding less drastic routes to reduce EGFR expression is desirable.
  
  
  METHODS
  Four glycosylation mutants of Chinese hamster ovary (CHO) cells with defects in N-glycan processing and branch-end maturation were tested for EGFR gene expression, production, functionality and routing after transfection with a vector encoding for human EGFR.
  
  
  RESULTS
  Lack of conversion of paucimannosidic to hybrid/complex-type N-glycans and drastic reductions in sialylation/galactosylation did not lead to major effects. In contrast, EGFR expression in a mutant with reduced presence of β1,4-galactosyltransferases-I-VI was markedly reduced. Misrouting or defects in transfection/transcription were excluded.
  
  
  CONCLUSIONS
  β1,4-Galactosyl-transferases warrant for further attention as effector(s) in order to attenuate EGFR-dependent signaling.
authors.affiliation: Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Veterinärstr. 13, D-80539 Munich, Germany. gabius@tiph.vetmed.uni-muenchen.de
authors.forename: Hans-J
authors.initials: HJ
authors.lastname: Gabius
authors.forename: Marlies
authors.initials: M
authors.lastname: van de Wouwer
authors.forename: Sabine
authors.initials: S
authors.lastname: André
authors.forename: Antonio
authors.initials: A
authors.lastname: Villalobo
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D015536:Down-Regulation; D066246:ErbB Receptors / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000502:physiology; D005700:Galactosyltransferases / Q000502:physiology*; D006031:Glycosylation; D057809:HEK293 Cells; D006801:Humans; D010766:Phosphorylation; D014162:Transfection
chemical_list: D005700:Galactosyltransferases; C493310:beta1,4-galactosyltransferase, human; D066246:ErbB Receptors