title: Isolation and identification of Escherichia albertii from a patient in an outbreak of gastroenteritis.
abstract: A microbial strain harboring the eae gene, which is known as the virulence gene  of enteropathogenic Escherichia coli (EPEC) and most enterohemorrhagic E. coli, was isolated  from a patient in a gastroenteritis outbreak that occurred in 22 patients in Akita Prefecture,  Japan, in November 2011. The biochemical characteristics of the isolate were more similar to  those of a novel Escherichia sp., E. albertii than E. coli. Partial 16S rRNA gene sequences of  the isolate were identical to those of a certain E. albertii strain, but also showed a high  degree of similarity to those of E. coli strains. Finally, we identified this isolate as E.  albertii by performing PCR analysis that targeted the uidA, lysP, mdh, and cdtB genes  in addition to stx and eae genes to differentiate between the EPEC and E. albertii strains.
authors.affiliation: Health and Hygiene Division, Akita Prefectural Research Center for Public Health and Environment, Akita 010-0874, Japan. konno-takayuki＠pref.akita.lg.jp
authors.forename: Takayuki
authors.initials: T
authors.lastname: Konno
authors.forename: Jun
authors.initials: J
authors.lastname: Yatsuyanagi
authors.forename: Shiho
authors.initials: S
authors.lastname: Takahashi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kumagai
authors.forename: Eriko
authors.initials: E
authors.lastname: Wada
authors.forename: Machiko
authors.initials: M
authors.lastname: Chiba
authors.forename: Shioko
authors.initials: S
authors.lastname: Saito
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004196:Disease Outbreaks; D004925:Escherichia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics; D005759:Gastroenteritis / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D012336:RNA, Ribosomal, 16S