title: Prevalence of the plasmid-mediated quinolone resistance genes, aac(6')-Ib-cr, qepA, and oqxAB in clinical isolates of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae in Korea.
abstract: In this study, we sought to determine the prevalence of the plasmid-mediated quinolone resistance (PMQR) genes aac(6')-Ib-cr, qepA, and oqxAB in extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae clinical isolates in South Korea. In total, 104 isolates (63 E. coli and 41 K. pneumoniae) were collected. We found that 23 of the 63 (36.5%) E. coli and nine of the 41 (22.0%) K. pneumoniae isolates were positive for aac(6')-Ib-cr. No isolate was positive for qepA, while transferable oqxAB was detected only in 10 (24.4%) K. pneumoniae isolates. Among the 32 aac(6')-Ib-cr-positive isolates, 30 (93.8%) were positive for both aac(6')-Ib-cr and bla(CTX-M) (CTX-M-15, -14, and -57). Our results suggest that PMQR determinants are highly prevalent in ESBL-producing E. coli and K. pneumoniae isolates in Korea.
authors.affiliation: Department of Laboratory Medicine, Kyung Hee University, School of Medicine, Seoul, South Korea.
authors.forename: Kyung Sun
authors.initials: KS
authors.lastname: Park
authors.forename: Myeong Hee
authors.initials: MH
authors.lastname: Kim
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Park
authors.forename: You Sun
authors.initials: YS
authors.lastname: Nam
authors.forename: Hee Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Suh
mesh_terms: D003227:Conjugation, Genetic / Q000187:drug effects; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D029968:Escherichia coli Proteins / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015363:Quinolones / Q000494:pharmacology; D056910:Republic of Korea; D001618:beta-Lactamases / Q000494:pharmacology*
chemical_list: D029968:Escherichia coli Proteins; C524438:QepA protein, E coli; D015363:Quinolones; D001618:beta-Lactamases