title: Tissue engineering of ureteral grafts by seeding urothelial differentiated hADSCs onto biodegradable ureteral scaffolds.
abstract: The study is aimed to evaluate the differentiation potential of human adipose-derived stem cells (hADSCs) into urothelial lineage, and to assess possibility of constructing ureteral grafts using the differentiated hADSCs and a novel polylactic acid (PLA)/collagen scaffolds. HADSCs were indirectly cocultured with urothelial cells in a transwell coculture system for urothelial differentiation. After 14 days coculturing, differentiation was evaluated by detecting urothelial lineage markers (cytokeratin-18 and uroplakin 2) in mRNA and protein level. Then the differentiated hADSCs were seeded onto PLA/collagen ureteral scaffolds and cultured in vitro for 1 week. The biocompatibility of the scaffolds was tested by scanning electron microscopy (SEM) and MTT analysis. At last, the cell/scafflod grafts were subcutaneously implanted into 4-week-old female athymic mice for 14 days. The results demonstrated that the hADSCs could be efficiently induced into urothelial lineage by indirect coculture. The differentiated cells seeded onto the PLA/collagen ureteral scaffolds survived up to 7 days and maintained proliferation in vitro, which indicated that the scaffolds displayed good biocompatibility. In vivo study showed that the differentiated cells in the grafts survived, formed multiple layers on the scaffolds and expressed urothelial lineage markers. In conclusion, hADSCs may serve as an alternative cell resource in cell-based tissue engineering for ureteral reconstruction. These cells could be employed to construct a model of ureteral engineering grafts and be effectively applied in vivo, which could be a new strategy on ureteral replacement with applicable potential in clinical research.
authors.affiliation: Department of Urology, Chinese People's Liberation Army General Hospital, Military Postgraduate Medical College, Haidian District, Beijing, People's Republic of China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Shi
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Fu
authors.forename: Xiao-Xiong
authors.initials: XX
authors.lastname: Wang
authors.forename: Yong-De
authors.initials: YD
authors.lastname: Xu
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Bao-Fa
authors.initials: BF
authors.lastname: Hong
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhi-Yan
authors.initials: ZY
authors.lastname: Du
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000328:Adult; D000368:Aged; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001673:Biodegradation, Environmental; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D019070:Cell Lineage / Q000187:drug effects; D002469:Cell Separation; D058948:Cell Tracking; D018920:Coculture Techniques; D005260:Female; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008875:Middle Aged; D019919:Prosthesis Implantation; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014513:Ureter / Q000637:transplantation* / Q000648:ultrastructure; D019459:Urothelium / Q000166:cytology*; D055815:Young Adult
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers