title: Chemokine and Fgf signalling act as opposing guidance cues in formation of the lateral line primordium.
abstract: The directional migration of many cell populations occurs as a coherent group. An amenable model is provided by the posterior lateral line in zebrafish, which is formed by a cohesive primordium that migrates from head to tail and deposits future neuromasts at intervals. We found that prior to the onset of migration, the compact state of the primordium is not fully established, as isolated cells with lateral line identity are present caudal to the main primordium. These isolated cells are retained in position such that they fuse with the migrating primordium as it advances, and later contribute to the leading zone and terminal neuromasts. We found that the isolated lateral line cells are positioned by two antagonistic cues: Fgf signalling attracts them towards the primordium, which counteracts Sdf1α/Cxcr4b-mediated caudal attraction. These findings reveal a novel chemotactic role for Fgf signalling in which it enables the coalescence of the lateral line primordium from an initial fuzzy pattern into a compact group of migrating cells.
authors.affiliation: Division of Developmental Neurobiology, MRC National Institute for Medical Research, London NW7 1AA, UK.
authors.forename: Marie A
authors.initials: MA
authors.lastname: Breau
authors.forename: Duncan
authors.initials: D
authors.lastname: Wilson
authors.forename: David G
authors.initials: DG
authors.lastname: Wilkinson
authors.forename: Qiling
authors.initials: Q
authors.lastname: Xu
mesh_terms: D000818:Animals; D002459:Cell Fusion; D002465:Cell Movement; D002469:Cell Separation; D018925:Chemokines / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D053403:Lateral Line System / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D008954:Models, Biological; D054387:Receptors, CXCR / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D015398:Signal Transduction; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000378:metabolism
chemical_list: C471276:CXCR4b protein, zebrafish; C519524:CXCR7 protein, zebrafish; D018925:Chemokines; D054387:Receptors, CXCR; D019718:Receptors, CXCR4; D029961:Zebrafish Proteins; D005346:Fibroblast Growth Factors