title: Backbone ¹H, ¹³C and ¹⁵N resonance assignments of dengue virus NS2B-NS3p in complex with aprotinin.
abstract: Dengue virus, belongs to Flaviviridae, is an arthropod transmitted virus that threatens millions of people's lives. As with other flaviviruses, a positive single-stranded 11-kilobases RNA in the dengue virus genome encodes three structural proteins (capsid protein C, membrane protein M, and envelope protein E) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). The two component protease NS2B-NS3p is essential for viral replication and is believed to be a potential antiviral drug target. Aprotinin, a native inhibitor, is proved to retard the activity of NS2B-NS3p. The backbone assignments of NS2B-NS3p will be essential for determining the high resolution solution structure of NS2B-NS3p and screening new antiviral drugs. Herein, we report the backbone (1)H, (15)N, (13)C resonance assignments of the N terminal fragment of NS2B (4.8 kDa) and NS3p (18.5 kDa) in complex with aprotinin (6.5 kDa) by high resolution NMR.
authors.affiliation: High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China.
authors.forename: Yunchen
authors.initials: Y
authors.lastname: Bi
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Jinsong
authors.initials: J
authors.lastname: Liu
authors.forename: Junfeng
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D007611:Aprotinin / Q000737:chemistry* / Q000378:metabolism; D002247:Carbon Isotopes; D003716:Dengue Virus / Q000378:metabolism*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D011522:Protons; D020365:RNA Helicases / Q000737:chemistry / Q000378:metabolism; D012697:Serine Endopeptidases / Q000737:chemistry / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000378:metabolism*; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D007611:Aprotinin; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011522:Protons; D020365:RNA Helicases; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases