title: Inhibitory effect of Psidium guajava water extract in the development of 2,4-dinitrochlorobenzene-induced atopic dermatitis in NC/Nga mice.
abstract: Atopic dermatitis (AD) is a chronic, relapsing, and inflammatory skin disease associated with eczematous symptoms and IgE hyperproduction. Psidium guajava is an important food crop and medicinal plant with anti-oxidant, anti-inflammatory, and anti-allergic activities, supporting its traditional uses. Our previous studies have shown that P. guajava extract inhibits Th2 chemokine expression by suppressing the activation of NF-κB and STAT1 co-stimulated with TNF-α and INF-γ. In this study, we investigated the inhibitory effect of P. guajava water extract (PGW) on 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions in NC/Nga mice. Treatment of cream containing PGW onto DNCB-induced AD-like skin lesions in NC/Nga mice ameliorated lesion intensity scores, levels of IgE, thymus and activation-regulated chemokine (TARC), TNF-α, and IL-4 in serum and ears. In contrast, PGW increased level of the immunosuppressive cytokine IL-10. Histological analyses demonstrated decreased thickening of the epidermis/dermis as well as dermal infiltration by inflammatory cells. These results suggest that cream containing PGW may be a potential therapeutic modality for AD and adjunctive agent to control pruritus in AD.
authors.affiliation: Department of Toxicology, College of Pharmacy, Chungnam National University, 220 Gung-dong, Daejeon 305-764, Republic of Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Choi
authors.forename: Bong Hwan
authors.initials: BH
authors.lastname: Park
authors.forename: Hyung Gyun
authors.initials: HG
authors.lastname: Kim
authors.forename: Yong Pil
authors.initials: YP
authors.lastname: Hwang
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Han
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Jin
authors.forename: Jong Kwon
authors.initials: JK
authors.lastname: Seo
authors.forename: Young Chul
authors.initials: YC
authors.lastname: Chung
authors.forename: Hye Gwang
authors.initials: HG
authors.lastname: Jeong
mesh_terms: D000818:Animals; D001483:Base Sequence; D016207:Cytokines / Q000378:metabolism; D017931:DNA Primers; D003876:Dermatitis, Atopic / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D004137:Dinitrochlorobenzene / Q000633:toxicity*; D007073:Immunoglobulin E / Q000378:metabolism; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D031647:Psidium / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014867:Water / Q000737:chemistry
chemical_list: D016207:Cytokines; D017931:DNA Primers; D004137:Dinitrochlorobenzene; D010936:Plant Extracts; D014867:Water; D007073:Immunoglobulin E