title: PCR detection of Burkholderia cepacia complex as one of key factors to handle a long-term outbreak.
abstract: BACKGROUND
  Once the outbreak with Burkholderia cenocepacia ST32 was identified in the Prague cystic fibrosis (CF) centre, molecular tools were implemented into diagnostic routine in order to complement infection control measures with as accurate as possible microbiological service. In addition, genotyping techniques were applied as part of an infection surveillance program to assign species and strain status in samples positive for Burkholderia cepacia complex (Bcc). We sought to evaluate a usefulness of Bcc-specific PCR in infection control and to map evolution of the outbreak.
  
  
  METHODS
  Since 2001, 6109 respiratory samples from 299 CF patients were examined not only by conventional culture, but also by PCR, detecting Bcc directly in sputum.
  
  
  RESULTS
  Diagnosis of Bcc infection was established by culture in 54 patients already prior to 2001. As 39 more patients were diagnosed by culture and/or PCR during 2001-2010, this represented annual prevalence of 18.5%-28.9%. Twelve of 39 patients were culture negative at the time of their first PCR positivity. Although 2/3 of them became subsequently culture positive, the time delay in diagnostics of the infection by culture ranged from 1 to 22 months. New cases of Bcc infection were detected every year, however a dramatic drop was observed for the epidemic strain ST32.
  
  
  CONCLUSIONS
  A likely factor contributing to the end of ST32 epidemic was segregation of Bcc infected patients that included also patients with no culture, but PCR positivity. The diagnostic PCR led to timely identification of cases with 'culture-invisible' infection.
authors.affiliation: Department of Paediatrics, 2nd Faculty of Medicine, Charles University and University Hospital Motol, V Uvalu 84, Prague 5, 150 06, Czech Republic.
authors.forename: Klara
authors.initials: K
authors.lastname: Dedeckova
authors.forename: Libor
authors.initials: L
authors.lastname: Fila
authors.forename: Veronika
authors.initials: V
authors.lastname: Skalicka
authors.forename: Jana
authors.initials: J
authors.lastname: Bartosova
authors.forename: Tereza
authors.initials: T
authors.lastname: Kucerova
authors.forename: Vera
authors.initials: V
authors.lastname: Vavrova
authors.forename: Dana
authors.initials: D
authors.lastname: Zemkova
authors.forename: Lucie
authors.initials: L
authors.lastname: Kalferstova
authors.forename: Oto
authors.initials: O
authors.lastname: Melter
authors.forename: Ondrej
authors.initials: O
authors.lastname: Cinek
authors.forename: Pavel
authors.initials: P
authors.lastname: Drevinek
mesh_terms: D000328:Adult; D015373:Bacterial Typing Techniques; D019121:Burkholderia Infections / Q000175:diagnosis / Q000453:epidemiology / Q000517:prevention & control; D042602:Burkholderia cepacia complex / Q000235:genetics / Q000302:isolation & purification; D003550:Cystic Fibrosis / Q000453:epidemiology / Q000382:microbiology; D018153:Czech Republic / Q000453:epidemiology; D004196:Disease Outbreaks / Q000517:prevention & control / Q000706:statistics & numerical data; D005260:Female; D006801:Humans; D017053:Infection Control / Q000379:methods; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000382:microbiology / Q000517:prevention & control; D013183:Sputum / Q000382:microbiology