title: Immunohistochemical staining of surfactant proteins A and B in skin of psoriatic patients before and after narrow-band UVB phototherapy.
abstract: BACKGROUND
  Psoriasis is a chronic inflammatory disorder that is mediated by elements of the innate and adaptive immune systems. Surfactant proteins (SPs) play an important role in host defense mechanisms. They are thought to have a potential role in some inflammatory skin diseases including psoriasis.
  
  
  OBJECTIVE
  The aim of the study was to evaluate SP-A and SP-B immunohistochemical staining in skin of psoriatic patients before and after narrow-band UV radiation type B (NB-UVB) phototherapy.
  
  
  METHODS
  Immunohistochemical staining for SP-A and SP-B was performed on tissues from 20 psoriatic patients before and after NB-UVB. Results were compared with the degree of improvement assessed by the Psoriasis Area and Severity Index (PASI) and duration of treatment.
  
  
  RESULTS
  In unaffected skin, SP-A and SP-B were restricted to the basal layer; however, in psoriatic skin, they appeared in suprabasal layers in 80% and 85% of cases, respectively. Dermal inflammatory cells showed SP-A in 11 cases (55%) and SP-B in only one case (5%). After treatment by NB-UVB, SP-A and SP-B staining showed predilection to the basal layer. Absence of SP-A staining in suprabasal layers after NB-UVB therapy was correlated to better response to therapy (p=0.003) and shorter duration of treatment (p<0.0001).
  
  
  CONCLUSIONS
  SP-A and SP-B positivity is increased in psoriatic skin and reduced after NB-UVB therapy. Absence of SP-A in suprabasal layers after NB-UVB therapy is associated with better response and shorter duration of treatment.
authors.affiliation: Pathology Department, Faculty of Medicine, Menoufiya University, Menoufiya, Egypt. hayamaiad@yahoo.com
authors.forename: Hayam Abdel Samie
authors.initials: HA
authors.lastname: Aiad
authors.forename: Shawky Mahmoud
authors.initials: SM
authors.lastname: El-Farargy
authors.forename: Mostafa Mohamed
authors.initials: MM
authors.lastname: Soliman
authors.forename: Mohamed Abd
authors.initials: MA
authors.lastname: El-Wahed Gaber
authors.forename: Shaymaa Abd
authors.initials: SA
authors.lastname: El-Aziz Othman
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000378:metabolism* / Q000532:radiotherapy*; D037662:Pulmonary Surfactant-Associated Protein A / Q000378:metabolism*; D037701:Pulmonary Surfactant-Associated Protein B / Q000378:metabolism*; D012720:Severity of Illness Index; D014467:Ultraviolet Therapy; D055815:Young Adult
chemical_list: D037662:Pulmonary Surfactant-Associated Protein A; D037701:Pulmonary Surfactant-Associated Protein B