title: Expression of BMP-2 and Ets1 in BMP-2-stimulated mouse pre-osteoblast differentiation is regulated by microRNA-370.
abstract: MicroRNAs (miRs) regulate several biological functions such as cell growth, cell differentiation, and carcinogenesis, by binding to the 3'-untranslated regions (3'-UTR) of specific target genes, in order to repress translation or promote degradation of the transcribed mRNAs. In the present study, using microRNA array and in silico analyses, we found that miR-370 regulates the expression of bone morphogenetic protein-2 (BMP-2) and V-ets Erythroblastosis Virus E26 Oncogene Homolog 1 (Ets1) in BMP-2-stimulated murine pre-osteoblast MC3T3-E1 cell differentiation. The enforced expression of mature miR-370 in MC3T3-E1 cells or primary osteoblast cells remarkably attenuated BMP-2-induced pre-osteoblast differentiation. To ascertain the mechanisms underlying the regulation of osteoblast differentiation by miR-370, we hypothesized a BMP-2-Ets1-PTHrP feed-forward loop regulatory mechanism.
authors.affiliation: Laboratory of Aquatic Food Science, Department of Fisheries, Faculty of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan. titoh@nara.kindai.ac.jp
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Itoh
authors.forename: Masashi
authors.initials: M
authors.lastname: Ando
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Tsukamasa
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akao
mesh_terms: D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D020869:Gene Expression Profiling; D017930:Genes, Reporter; D049410:Luciferases, Renilla / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010006:Osteoblasts / Q000502:physiology*; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D050784:Proto-Oncogene Protein c-ets-1 / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D014158:Transcription, Genetic
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; C494042:Ets1 protein, mouse; C551449:MIRN370 microRNA, mouse; D035683:MicroRNAs; D050784:Proto-Oncogene Protein c-ets-1; C493716:Runx2 protein, mouse; D053495:Osteopontin; D049410:Luciferases, Renilla