title: Plasma polymer films as an alternative to (PSS-PAH)n or (PSS-PDADMAC)n films to retain active enzymes in exponentially growing polyelectrolyte multilayers.
abstract: Compact and linearly growing polyelectrolyte multilayer films have been used to suppress desorption of drugs, nanoparticles or proteins from underlying polyelectrolyte multilayer films as well as to significantly change their mechanical properties. The polyelectrolyte based capping layers are however cumbersome to deposit and alternative barrier layers offering enzymatic retention in the films as well as permeability to small molecules, for example the substrates of the embedded enzymes, are highly desired. In this article we show that barrier layers made through atmospheric pressure dielectric barrier discharge polymerization of ethylene glycol dimethacrylate offer the opportunity to simultaneously suppress enzyme desorption from the underlying polyelectrolyte multilayer film and to ensure accessibility of the enzymatic substrate. This holds true when the barrier film totally covers the underlying film and as long is not too thick. When the plasma deposited barrier becomes 300 nm thick, the hydrolysis curve of the enzyme presents a lag phase typical of a diffusion-limited process.
authors.affiliation: Advanced Materials and Structures, Centre de Recherche Public Henri Tudor, Esch-sur-Alzette, Luxembourg.
authors.forename: Cédric
authors.initials: C
authors.lastname: Amorosi
authors.forename: Marc
authors.initials: M
authors.lastname: Michel
authors.forename: Luc
authors.initials: L
authors.lastname: Avérous
authors.forename: Valérie
authors.initials: V
authors.lastname: Toniazzo
authors.forename: David
authors.initials: D
authors.lastname: Ruch
authors.forename: Vincent
authors.initials: V
authors.lastname: Ball
mesh_terms: D008567:Membranes, Artificial; D011095:Polyethylenes / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D011095:Polyethylenes; D011108:Polymers; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride