title: Imaging of metal bioaccumulation in hay-scented fern (Dennstaedtia punctilobula) rhizomes growing on contaminated soils by laser ablation ICP-MS.
abstract: Understanding Pb removal from the translocation stream is vital to engineering Pb hyperaccumulation in above ground organs, which would enhance the economic feasibility of Pb phytoextraction technologies. We investigated Cu, Pb, Sb and Zn distributions in Hay-scented fern (Dennstaedtia punctilobula) rhizomes on shooting range soils by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), analyzing digested rhizomes, stems, and fronds using ICP-MS. Nutrients Cu and Zn concentrated in fronds while toxic elements Pb and Sb did not, showing potential Pb and Sb sequestration in the rhizome. Frond and rhizome concentration of Pb was 0.17 ± 0.10% and 0.32 ± 0.21% of dry biomass, respectively. The 208Pb/13C and 121Sb/13C determined by LA-ICP-MS increased from inner sclerotic cortex to the epidermis, while Pb concentrated in the starchy cortex only in contaminated sites. These results suggest that concentration dependent bioaccumulation in the rhizome outer cortex removes Pb from the vascular transport stream.
authors.affiliation: School of Natural Science, Hampshire College, 869, West Street, Amherst, MA 01002, USA.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Koelmel
authors.forename: Dulasiri
authors.initials: D
authors.lastname: Amarasiriwardena
mesh_terms: D000965:Antimony / Q000032:analysis; D001673:Biodegradation, Environmental; D003300:Copper / Q000032:analysis; D029624:Ferns / Q000737:chemistry* / Q000254:growth & development; D007854:Lead / Q000032:analysis; D013058:Mass Spectrometry; D008670:Metals / Q000032:analysis*; D027343:Rhizome / Q000737:chemistry*; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis*; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000032:analysis
chemical_list: D008670:Metals; D012987:Soil; D012989:Soil Pollutants; D007854:Lead; D003300:Copper; D000965:Antimony; D015032:Zinc