title: A study on L-asparaginase of Nocardia levis MK-VL_113.
abstract: An enzyme-based drug, L-asparaginase, was produced by Nocardia levis MK-VL_113 isolated from laterite soils of Guntur region. Cultural parameters affecting the production of L-asparaginase by the strain were optimized. Maximal yields of L-asparaginase were recorded from 3-day-old culture grown in modified asparagine-glycerol salts broth with initial pH 7.0 at temperature 30°C. Glycerol (2%) and yeast extract (1.5%) served as good carbon and nitrogen sources for L-asparaginase production, respectively. Cell-disrupting agents like EDTA slightly enhanced the productivity of L-asparaginase. Ours is the first paper on the production of L-asparaginase by N. levis.
authors.affiliation: Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur 522 510, India.
authors.forename: Alapati
authors.initials: A
authors.lastname: Kavitha
authors.forename: Muvva
authors.initials: M
authors.lastname: Vijayalakshmi
mesh_terms: D001215:Asparaginase / Q000494:pharmacology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D009615:Nocardia / Q000201:enzymology*
chemical_list: D001215:Asparaginase