title: Regulation of lung surfactant secretion by microRNA-150.
abstract: P2X7 receptor (P2X7R) is a purinergic ion-channel receptor. We have previously shown that the activation of P2X7R in alveolar type I cells stimulates surfactant secretion in alveolar type II cells. In this study, we determined whether miR-150 regulates P2X7R-mediated surfactant secretion. The miR-150 expression level in alveolar type II cells was much higher than alveolar type I cells, which was inversely correlated with the P2X7R protein level. An adenovirus expressing miR-150 significantly reduced the P2X7R protein expression in E10 cells, an alveolar type I cell line. Furthermore, pre-treatment of E10 cells with the adenovirus reduced the surfactant secretion induced by E10 cell conditioned medium. Our study demonstrates that miR-150 regulates surfactant secretion through P2X7R.
authors.affiliation: Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: Tingting
authors.initials: T
authors.lastname: Weng
authors.forename: Amarjit
authors.initials: A
authors.lastname: Mishra
authors.forename: Yujie
authors.initials: Y
authors.lastname: Guo
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Lijing
authors.initials: L
authors.lastname: Su
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Huang
authors.forename: Chunling
authors.initials: C
authors.lastname: Zhao
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D011650:Pulmonary Alveoli / Q000378:metabolism*; D037661:Pulmonary Surfactant-Associated Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D058486:Receptors, Purinergic P2X7 / Q000096:biosynthesis* / Q000235:genetics; D020545:Respiratory Mucosa / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D035683:MicroRNAs; D037661:Pulmonary Surfactant-Associated Proteins; D058486:Receptors, Purinergic P2X7; C533097:MIR150, human