title: A reliable and rapid tool for plasma quantification of 18 psychotropic drugs by ESI tandem mass spectrometry.
abstract: A simple liquid chromatographic tandem mass spectrometry (LC-MS/MS) method has been developed for simultaneous analysis of 17 basic and one acid psychotropic drugs in human plasma. The method relies on a protein precipitation step for sample preparation and offers high sensitivity, wide linearity without interferences from endogenous matrix components. Chromatography was run on a reversed-phase column with an acetonitrile-H₂O mixture. The quantification of target compounds was performed in multiple reaction monitoring (MRM) and by switching the ionization polarity within the analytical run. A further sensitivity increase was obtained by implementing the functionality "scheduled multiple reaction monitoring" (sMRM) offered by the recent version of the software package managing the instrument. The overall injection interval was less than 5.5 min. Regression coefficients of the calibration curves and limits of quantification (LOQ) showed a good coverage of over-therapeutic, therapeutic and sub-therapeutic ranges. Recovery rates, measured as percentage of recovery of spiked plasma samples, were ≥ 94%. Precision and accuracy data have been satisfactory for a therapeutic drug monitoring (TDM) service as for managing plasma samples from patients receiving psycho-pharmacological treatment.
authors.affiliation: Atherosclerosis and Thrombosis Unit, I.R.C.C.S. "Casa Sollievo della Sofferenza", S. Giovanni Rotondo, Foggia, Italy.
authors.forename: Gennaro
authors.initials: G
authors.lastname: Vecchione
authors.forename: Bruno
authors.initials: B
authors.lastname: Casetta
authors.forename: Antonella
authors.initials: A
authors.lastname: Chiapparino
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bertolino
authors.forename: Michela
authors.initials: M
authors.lastname: Tomaiuolo
authors.forename: Filomena
authors.initials: F
authors.lastname: Cappucci
authors.forename: Raffaella
authors.initials: R
authors.lastname: Gatta
authors.forename: Maurizio
authors.initials: M
authors.lastname: Margaglione
authors.forename: Elvira
authors.initials: E
authors.lastname: Grandone
mesh_terms: D014150:Antipsychotic Agents / Q000097:blood* / Q000627:therapeutic use; D002138:Calibration; D002853:Chromatography, Liquid; D006801:Humans; D001523:Mental Disorders / Q000097:blood / Q000188:drug therapy; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D014150:Antipsychotic Agents