title: Conjugation of functionalized SPIONs with transferrin for targeting and imaging brain glial tumors in rat model.
abstract: Currently, effective and specific diagnostic imaging of brain glioma is a major challenge. Nanomedicine plays an essential role by delivering the contrast agent in a targeted manner to specific tumor cells, leading to improvement in accurate diagnosis by good visualization and specific demonstration of tumor cells. This study investigated the preparation and characterization of a targeted MR contrast agent, transferrin-conjugated superparamagnetic iron oxide nanoparticles (Tf-SPIONs), for brain glioma detection. MR imaging showed the obvious contrast change of brain glioma before and after administration of Tf-SPIONs in C6 glioma rat model in vivo on T2 weighted imaging. Significant contrast enhancement of brain glioma could still be clearly seen even 48 h post injection, due to the retention of Tf-SPIONs in cytoplasm of tumor cells which was proved by Prussian blue staining. Thus, these results suggest that Tf-SPIONs could be a potential targeting MR contrast agent for the brain glioma.
authors.affiliation: Radiology Department of Union Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Weili
authors.initials: W
authors.lastname: Jiang
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Devina
authors.initials: D
authors.lastname: Ghoorah
authors.forename: Yalei
authors.initials: Y
authors.lastname: Shang
authors.forename: Haojun
authors.initials: H
authors.lastname: Shi
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Xiangliang
authors.initials: X
authors.lastname: Yang
authors.forename: Haibo
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000175:diagnosis*; D003287:Contrast Media / Q000008:administration & dosage; D003911:Dextrans; D005290:Ferric Compounds; D005910:Glioma / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D008297:Male; D059906:Neuroimaging / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D014168:Transferrin
chemical_list: D003287:Contrast Media; D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D014168:Transferrin; C000499:ferric oxide; C065507:ferumoxides