title: Comparison of the direct electrochemistry of glucose oxidase immobilized on the surface of Au, CdS and ZnS nanostructures.
abstract: A comparison of the electrochemical and photoelectrochemical behaviors of three biosensors, based on the use of Au, CdS, and ZnS nanoparticles-glucose oxidase (GOD) system, is discussed. All the nanoparticles were electrodeposited onto the indium tin oxide (ITO) thin film coated glass surface. GOD was then immobilized on the nanoparticles-modified electrodes surface with the sol-gel technique. The deposited nanoparticles on ITO electrodes were characterized by scanning electron microscopy, UV-vis spectroscopy and electrochemical impedance spectra. The direct electrochemistry of GOD, analytical performance of glucose calibration curves and the kinetic parameters of the enzyme reaction were compared for all the electrochemical biosensors. Furthermore, the current response of the quantum dots-GOD system biosensor can be increased after illumination. The electrochemical and photoelectrochemical biosensors based on ZnS nanostructures exhibited higher sensitivity than that of Au or CdS nanostructures. Considering ZnS is nontoxic to human and environment, the results suggest that ZnS nanoparticles-GOD system seems to be a promising platform for fabrication of novel electrochemical and photoelectrochemical biosensors.
authors.affiliation: College of Chemistry, Chemical Engineering and Material Science, Soochow University, Suzhou, Jiangsu 215123, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Du
authors.forename: Xiuping
authors.initials: X
authors.lastname: Yu
authors.forename: Junwei
authors.initials: J
authors.lastname: Di
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D019187:Cadmium Compounds / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013440:Sulfides / Q000737:chemistry; D017971:Tin Compounds / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D019187:Cadmium Compounds; D004800:Enzymes, Immobilized; D013440:Sulfides; D017971:Tin Compounds; D017967:Zinc Compounds; C034939:cadmium sulfide; C109984:indium tin oxide; D006046:Gold; D005949:Glucose Oxidase; C031238:zinc sulfide