title: Microchip electrophoresis, with respect to "profiling of Aβ peptides in the cerebrospinal fluid of patients with Alzheimer's disease".
abstract: Aggregation of beta amyloid peptides especially Aβ1-42 in amyloid plaques is one of the major -neuropathological events in Alzheimer's disease. This event is normally accompanied by a relative reduction of the concentration of Aβ1-42 in the cerebrospinal fluid (CSF) of patients developing the signs of Alzheimer's disease. Here, we describe a microchip gel electrophoresis method in a polydimethylsiloxane (PDMS) chip that enables rapid profiling of major Aβ peptides. The method was applied to compare the relative concentration of Aβ1-42 with other Aβ peptides, for example, Aβ 1-40 in CSF. In order to increase the sensitivity of detection, Aβ peptides in the CSF samples were first captured and concentrated using magnetic beads coated with specific anti-Aβ antibodies.
authors.affiliation: UMR 168, Curie Institute/CNRS/Université Pierre et Marie Curie, Paris, France.
authors.forename: Mohamad Reza
authors.initials: MR
authors.lastname: Mohamadi
authors.forename: Romain
authors.initials: R
authors.lastname: Verpillot
authors.forename: Myriam
authors.initials: M
authors.lastname: Taverna
authors.forename: Markus
authors.initials: M
authors.lastname: Otto
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Viovy
mesh_terms: D000544:Alzheimer Disease / Q000134:cerebrospinal fluid*; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid* / Q000737:chemistry / Q000302:isolation & purification; D002021:Buffers; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D012015:Reference Standards; D013194:Staining and Labeling
chemical_list: D016229:Amyloid beta-Peptides; D002021:Buffers; D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; C013830:baysilon