title: Antiproliferative Properties of Clausine-B against Cancer Cell Lines.
abstract: BACKGROUND
  CLAUSINE B, A CARBAZOLE ALKALOID ISOLATED FROM THE STEM BARK OF CLAUSENA EXCAVATA, WAS INVESTIGATED FOR ITS ANTIPROLIFERATIVE ACTIVITIES AGAINST FIVE HUMAN CANCER CELL LINES: HepG2 (hepatic cancer), MCF-7 (hormone-dependent breast cancer), MDA-MB-231 (non-hormone-dependent breast cancer), HeLa (cervical cancer), and CAOV3 (ovarian cancer).
  
  
  METHODS
  Chang liver (normal cells) was used as a control. The effect of clausine-B was measured using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.
  
  
  RESULTS
  Clausine-B was found to be active (IC(50)<30 μg/mL) against four of the cancer cell lines tested. The IC(50) values for these four lines were: 21.50 μg/mL (MDA-MB-231), 22.90 g/ml (HeLa), 27.00 μg/mL (CAOV3) and 28.94 μg/mL (HepG2). Clausine-B inhibited the MCF-7 cancer cell line at 52.90 μg/mL, and no IC(50) value was obtained against Chang liver.
  
  
  CONCLUSIONS
  It is possible that the phenolic group in clausine-B responsible for the antiproliferative activities found in this study.
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Wan Nor I'zzah
authors.initials: WN
authors.lastname: Wan Mohd Zain
authors.forename: Asmah
authors.initials: A
authors.lastname: Rahmat
authors.forename: Fauziah
authors.initials: F
authors.lastname: Othman
authors.forename: Taufiq Yun Hin
authors.initials: TY
authors.lastname: Yap