title: One-step sequence- and ligation-independent cloning as a rapid and versatile cloning method for functional genomics studies.
abstract: We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.
authors.affiliation: Marine Biotechnology Research Center, Korea Ocean Research & Development Institute, Ansan, South Korea.
authors.forename: Jae-Yeon
authors.initials: JY
authors.lastname: Jeong
authors.forename: Hyung-Soon
authors.initials: HS
authors.lastname: Yim
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Ryu
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Lee
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Dong-Seung
authors.initials: DS
authors.lastname: Seen
authors.forename: Sung Gyun
authors.initials: SG
authors.lastname: Kang
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D023281:Genomics / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014169:Transformation, Bacterial / Q000235:genetics*; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D014764:Viral Proteins; C069983:gene 43 protein, Enterobacteria phage T4; D004259:DNA-Directed DNA Polymerase