title: Relative intensities of recognition factors at two combining sites of Ralstonia solanacearum lectin (RSL) for accommodating LFucα1-->, DManα1--> and Galβ1-->3/4GlcNAc glycotopes.
abstract: Owing to the weak reactivities of monomeric DManα1 and Galβ1-->3/4GlcNAcβ (I(β)/II(β)) glycotopes with Ralstonia solanacearum lectin (RSL), their recognition roles were previously ignored. In this study, the interaction intensities of RSL toward four monomeric glycotopes LFucα1-->, DManα1--> and I(β)/II(β) within two combining sites were established by both enzyme-linked lectinosorbent and inhibition assays. It was found that high density of LFucα1--> complex enhanced the recognition intensities at LFucα1--> site, polyvalent DManα1--> was essential for binding at the DManα1--> site and polyvalent I(β)/II(β) was required at LFucα1--> site. The peculiar recognition systems of RSL are very different from other well known microbial lectins.
authors.affiliation: Glyco-Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang-Gung University, Kwei-san, Tao-yuan 333, Taiwan. amwu@mail.cgu.edu.tw
authors.forename: Albert M
authors.initials: AM
authors.lastname: Wu
authors.forename: Jia-Hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Anthony
authors.initials: A
authors.lastname: Herp
authors.forename: Dvora
authors.initials: D
authors.lastname: Sudakevitz
authors.forename: Nechama
authors.initials: N
authors.lastname: Gilboa-Garber
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000378:metabolism; D008351:Mannans / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D043368:Ralstonia solanacearum; D034781:Receptors, N-Acetylglucosamine / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D008351:Mannans; D034781:Receptors, N-Acetylglucosamine; D000117:Acetylglucosamine