title: Removing heavy metals by in vitro cultures.
abstract: In vitro roots cultures of Typha latifolia and Scirpus americanus aquatic plants have the capacity to remove Pb (II), Mn (II), and Cr (III) from the culture medium. Both species remove Cr and Pb by an absorption process, while Mn is mainly adsorbed to the root surface. This chapter describes a protocol for the establishment of in vitro roots cultures (nontransformed) from T. latifolia and S. americanus, and the procedure for the uptake analysis of Pb (II), Mn (II), and Cr (III) by roots.
authors.affiliation: Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, México. ssantos@uaslp.mx
authors.forename: María del Socorro
authors.initials: Mdel S
authors.lastname: Santos-Díaz
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Barrón-Cruz
mesh_terms: D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry; D029785:Cyperaceae / Q000737:chemistry* / Q000254:growth & development; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000032:analysis* / Q000378:metabolism; D019216:Metals, Heavy / Q000032:analysis* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry* / Q000254:growth & development; D046509:Tissue Culture Techniques; D032401:Typhaceae / Q000737:chemistry* / Q000254:growth & development
chemical_list: D003470:Culture Media; D004785:Environmental Pollutants; D019216:Metals, Heavy