title: Intratracheal instillation of lipopolymeric vectors and the effect on mice lung physiology.
abstract: OBJECTIVE
  The current study compared the effects of intratracheal administration of different lipopolymeric vectors on lung function and histology in normal mice.
  
  
  METHODS
  Forty-eight BALB/c mice were randomly divided into 8 groups (6/group). All animals received intratracheal instillation of the following suspensions: polymerized [(A) 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC):1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine (DC8,9PC):1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), (B) DMPC:DC8,9PC:stearylamine (SA), (C) DMPC:DC8,9PC:myristoylcholine chloride (MCl)]; nonpolymerized [(D) DMPC:DC8,9PC:DOTAP, (E) DMPC:DC8,9PC:SA, (F) DMPC:DC8,9PC:MCl] together with plasmid DNA; vehicle (control), and pDsRed2-N1 plasmid DNA (DNA). At 24 h, the survival rate, lung mechanics (resistive and viscoelastic pressure, static elastance) and morphometry were analyzed.
  
  
  RESULTS
  The survival rate was 50% in D, 40% in E and F, and 100% in the CTRL, DNA, A, B and C groups. Animals from groups D, E, and F that died presented diffuse pulmonary hemorrhagic capillaritis. Lung mechanics, the fraction of normal and collapsed alveoli, as well as the number of polymorphonuclear and mononuclear cells in lung tissue were similar in all surviving mice.
  
  
  CONCLUSIONS
  Intratracheal instillation of polymerized particles is safe compared with nonpolymerized formulations and may be used for future gene/drug therapy.
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Debora G
authors.initials: DG
authors.lastname: Xisto
authors.forename: Carlos Facundo
authors.initials: CF
authors.lastname: Temprana
authors.forename: Sabrina V
authors.initials: SV
authors.lastname: Martini
authors.forename: Adriana L
authors.initials: AL
authors.lastname: Silva
authors.forename: Soraia G
authors.initials: SG
authors.lastname: Abreu
authors.forename: Johnatas D
authors.initials: JD
authors.lastname: Silva
authors.forename: Julia
authors.initials: J
authors.lastname: Crosseti
authors.forename: Patricia R M
authors.initials: PR
authors.lastname: Rocco
authors.forename: Silvia
authors.initials: S
authors.lastname: del Valle Alonso
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Morales
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004337:Drug Carriers; D051379:Mice; D008807:Mice, Inbred BALB C; D011108:Polymers / Q000008:administration & dosage*; D060888:Real-Time Polymerase Chain Reaction; D015656:Respiratory Mechanics; D013056:Spectrophotometry, Ultraviolet; D014132:Trachea
chemical_list: D017931:DNA Primers; D004337:Drug Carriers; D011108:Polymers