title: SPE-MS analysis of absorption, distribution, metabolism and excretion assays: a tool to increase throughput and steamline workflow.
abstract: In an effort to create faster and more efficient bioanalytical methods for drug development, many investigators have evaluated a variety of SPE-MS systems. Over the past 15 years online systems have evolved from run times of >1.5 min/sample to <10 s/sample. High-throughput SPE-MS methods for in vitro absorption, distribution, metabolism and excretion screening assays have been described by several laboratories and shown to produce results comparable to conventional LC-MS/MS systems. While quantitative analysis of small molecules in biological matrixes holds many challenges, for several applications SPE-MS methods have achieved comparable results to LC-MS/MS with the benefit of 10-30-times the throughput. Based on its distinct advantages of throughput and streamlined workflow efficiencies, SPE-MS is a useful tool for the analysis of many in vitro absorption, distribution, metabolism and excretion assays and in vivo bioanalytical studies. Further development of SPE-MS methods and analysis workflows has the potential to expand the capabilities of this technology for other challenging bioanalytical applications.
authors.affiliation: Agilent Technologies Inc., 11 Audubon Road, Wakefield, MA 01880-1256, USA. vaughn.miller@agilent.com
authors.forename: Vaughn P
authors.initials: VP
authors.lastname: Miller
mesh_terms: D000042:Absorption; D000818:Animals; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D010599:Pharmacokinetics; D052616:Solid Phase Extraction
chemical_list: D065607:Cytochrome P-450 Enzyme Inhibitors; D004364:Pharmaceutical Preparations; D003577:Cytochrome P-450 Enzyme System