title: Measurement of endo-1,4-β-glucanase.
abstract: Several procedures are available for the measurement of endo-1,4-β-glucanase (EG). Primary methods employ defined oligosaccharides or highly purified polysaccharides and measure the rate of hydrolysis of glycosidic bonds using a reducing-sugar method. However, these primary methods are not suitable for the measurement of EG in crude fermentation broths due to the presence of reducing sugars and other enzymes active on these substrates. In such cases, dyed soluble or insoluble substrates are preferred as they are specific, sensitive, easy to use, and are not affected by other components, such as reducing sugars, in the enzyme preparation.
authors.affiliation: Megazyme International Ireland, Bray Business Park, Bray, County Wicklow, Ireland.
authors.forename: Barry V
authors.initials: BV
authors.lastname: McCleary
authors.forename: Vincent
authors.initials: V
authors.lastname: McKie
authors.forename: Anna
authors.initials: A
authors.lastname: Draga
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D001407:Bacillus / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology*; D002480:Cellulase / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods*; D005285:Fermentation; D005658:Fungi / Q000201:enzymology*; D006801:Humans; D009844:Oligosaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D019163:Reducing Agents / Q000378:metabolism; D032901:Talaromyces / Q000201:enzymology; D020124:Thermotoga maritima / Q000201:enzymology; D014242:Trichoderma / Q000201:enzymology; D014783:Viscosity
chemical_list: D004396:Coloring Agents; D009844:Oligosaccharides; D011134:Polysaccharides; D019163:Reducing Agents; D002480:Cellulase