title: Galvestine-1, a novel chemical probe for the study of the glycerolipid homeostasis system in plant cells.
abstract: Plant cells are characterized by the presence of chloroplasts, membrane lipids of which contain up to ∼80% mono- and digalactosyldiacylglycerol (MGDG and DGDG). The synthesis of MGDG in the chloroplast envelope is essential for the biogenesis and function of photosynthetic membranes, is coordinated with lipid metabolism in other cell compartments and is regulated in response to environmental factors. Phenotypic analyses of Arabidopsis using the recently developed specific inhibitor called galvestine-1 complete previous analyses performed using various approaches, from enzymology, cell biology to genetics. This review details how this probe could be beneficial to study the lipid homeostasis system at the whole cell level and highlights connections between MGDG synthesis and Arabidopsis flower development.
authors.affiliation: Laboratoire de Physiologie Cellulaire Végétale, UMR 5168 CNRS-CEA-INRA-Université J. Fourier Grenoble 1, Grenoble, France. eric.marechal@cea.fr
authors.forename: Laurence
authors.initials: L
authors.lastname: Boudière
authors.forename: Cyrille Y
authors.initials: CY
authors.lastname: Botté
authors.forename: Nadia
authors.initials: N
authors.lastname: Saidani
authors.forename: Mathieu
authors.initials: M
authors.lastname: Lajoie
authors.forename: Jessica
authors.initials: J
authors.lastname: Marion
authors.forename: Laurent
authors.initials: L
authors.lastname: Bréhélin
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yamaryo-Botté
authors.forename: Béatrice
authors.initials: B
authors.lastname: Satiat-Jeunemaître
authors.forename: Christelle
authors.initials: C
authors.lastname: Breton
authors.forename: Agnès
authors.initials: A
authors.lastname: Girard-Egrot
authors.forename: Olivier
authors.initials: O
authors.lastname: Bastien
authors.forename: Juliette
authors.initials: J
authors.lastname: Jouhet
authors.forename: Denis
authors.initials: D
authors.lastname: Falconet
authors.forename: Maryse A
authors.initials: MA
authors.lastname: Block
authors.forename: Eric
authors.initials: E
authors.lastname: Maréchal
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism; D038983:Galactolipids / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism*; D006706:Homeostasis; D050356:Lipid Metabolism / Q000187:drug effects; D010880:Piperidines / Q000494:pharmacology*; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism*
chemical_list: D038983:Galactolipids; D005989:Glycerides; D010880:Piperidines; C568628:galvestine-1; C009909:monogalactosyldiacylglycerol