title: Verification of the role of MlrC in microcystin biodegradation by studies using a heterologously expressed enzyme.
abstract: The MlrC protein from Sphingomonas ACM-3962 strain was heterologously expressed in Escherichia coli strain BL21(DE3) and purified to investigate participation of this enzyme in the biodegradation of two microcystin variants. In contrast with previous reports, our results indicated that MlrC cleaves linear microcystins, thus shedding new light on the role of MlrC enzyme in microcystin biodegradation.
authors.affiliation: Department of Plant Physiology and Development, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Dziga
authors.forename: Marcin
authors.initials: M
authors.lastname: Wasylewski
authors.forename: Adam
authors.initials: A
authors.lastname: Szetela
authors.forename: Oliwia
authors.initials: O
authors.lastname: Bocheńska
authors.forename: Benedykt
authors.initials: B
authors.lastname: Wladyka
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D052998:Microcystins / Q000737:chemistry / Q000378:metabolism*; D015203:Reproducibility of Results; D020578:Sphingomonas; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D052998:Microcystins