title: Metabolomics: a valuable tool for stem cell monitoring in regenerative medicine.
abstract: Metabolomics is a method for investigation of changes in the global metabolite profile of cells. This paper discusses the technical application of the approach, considering metabolite extraction, separation, mass spectrometry and data interpretation. A particular focus is on the application of metabolomics to the study of stem cell physiology in the context of biomaterials and regenerative medicine. Case studies are used to illustrate key points, focusing on the use of metabolomics in the examination of mesenchymal stem cell responses to titania-nanopillared substrata designed for orthopaedic applications.
authors.affiliation: Centre for Cell Engineering, Division of Molecular, Cell, and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK. laura.mcnamara@glasgow.ac.uk
authors.forename: Laura E
authors.initials: LE
authors.lastname: McNamara
authors.forename: Terje
authors.initials: T
authors.lastname: Sjöström
authors.forename: R M Dominic
authors.initials: RM
authors.lastname: Meek
authors.forename: Richard O C
authors.initials: RO
authors.lastname: Oreffo
authors.forename: Bo
authors.initials: B
authors.lastname: Su
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
authors.forename: Karl E V
authors.initials: KE
authors.lastname: Burgess
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods*; D044968:Regenerative Medicine / Q000379:methods*; D014025:Titanium / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D014025:Titanium