title: Analysis of the structural and immunological stability of 2S albumin, nonspecific lipid transfer protein, and profilin allergens from mustard seeds.
abstract: This work investigates the resistance to proteolysis and heating of the yellow mustard (Sinapis alba L.) allergens Sin a 1 (2S albumin), Sin a 3 (nonspecific lipid transfer protein, LTP), and Sin a 4 (profilin) to explain their potential capability to induce primary sensitization at the gastrointestinal level. Sin a 1 and Sin a 3 resisted gastric digestion showing no reduction of the IgE reactivity. Intestinal digestion of Sin a 1 and Sin a 3 produced a limited proteolysis but retained significant IgE-binding reactivity. Sin a 1 was stable after heating, and although Sin a 3 was modified, most of its structure was recovered after cooling back. These two allergens would be therefore able to sensitize by ingestion. Sin a 4 was completely digested by gastric treatment and its conformational structure markedly modified at 85 °C. Thus, this allergen can be described as a nonsensitizing mustard allergen.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Química, Universidad Complutense de Madrid, Ciudad Universitaria, 28040 Madrid, Spain.
authors.forename: Sofía
authors.initials: S
authors.lastname: Sirvent
authors.forename: Oscar
authors.initials: O
authors.lastname: Palomares
authors.forename: Javier
authors.initials: J
authors.lastname: Cuesta-Herranz
authors.forename: Mayte
authors.initials: M
authors.lastname: Villalba
authors.forename: Rosalía
authors.initials: R
authors.lastname: Rodríguez
mesh_terms: D000418:Albumins / Q000276:immunology / Q000378:metabolism*; D000485:Allergens / Q000276:immunology / Q000378:metabolism*; D052179:Antigens, Plant / Q000276:immunology / Q000378:metabolism; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002942:Circular Dichroism; D004063:Digestion / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000378:metabolism; D015394:Molecular Structure; D009149:Mustard Plant / Q000737:chemistry*; D010940:Plant Proteins / Q000276:immunology / Q000378:metabolism; D051304:Profilins / Q000276:immunology / Q000378:metabolism*; D011487:Protein Conformation; D059748:Proteolysis / Q000187:drug effects; D012639:Seeds / Q000737:chemistry*; D017365:Spices / Q000032:analysis
chemical_list: D000418:Albumins; D000485:Allergens; D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; D051304:Profilins; C079479:Sin a I protein, Sinapis alba; C072087:sterol carrier proteins; D007073:Immunoglobulin E