title: Effects of aluminum oxide nanoparticles on the growth, development, and microRNA expression of tobacco (Nicotiana tabacum).
abstract: Nanoparticles are a class of newly emerging environmental pollutions. To date, few experiments have been conducted to investigate the effect nanoparticles may have on plant growth and development. It is important to study the effects nanoparticles have on plants because they are stationary organisms that cannot move away from environmental stresses like animals can, therefore they must overcome these stresses by molecular routes such as altering gene expression. microRNAs (miRNA) are a newly discovered, endogenous class of post-transcriptional gene regulators that function to alter gene expression by either targeting mRNAs for degradation or inhibiting mRNAs translating into proteins. miRNAs have been shown to mediate abiotic stress responses such as drought and salinity in plants by altering gene expression, however no study has been performed on the effect of nanoparticles on the miRNA expression profile; therefore our aim in this study was to classify if certain miRNAs play a role in plant response to Al(2)O(3) nanoparticle stress. In this study, we exposed tobacco (Nicotiana tabacum) plants (an important cash crop as well as a model organism) to 0%, 0.1%, 0.5%, and 1% Al(2)O(3) nanoparticles and found that as exposure to the nanoparticles increased, the average root length, the average biomass, and the leaf count of the seedlings significantly decreased. We also found that miR395, miR397, miR398, and miR399 showed an extreme increase in expression during exposure to 1% Al(2)O(3) nanoparticles as compared to the other treatments and the control, therefore these miRNAs may play a key role in mediating plant stress responses to nanoparticle stress in the environment. The results of this study show that Al(2)O(3) nanoparticles have a negative effect on the growth and development of tobacco seedlings and that miRNAs may play a role in the ability of plants to withstand stress to Al(2)O(3) nanoparticles in the environment.
authors.affiliation: Department of Biology, East Carolina University, Greenville, North Carolina, United States of America.
authors.forename: Caitlin E
authors.initials: CE
authors.lastname: Burklew
authors.forename: Jordan
authors.initials: J
authors.lastname: Ashlock
authors.forename: William B
authors.initials: WB
authors.lastname: Winfrey
authors.forename: Baohong
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000537:Aluminum Oxide / Q000633:toxicity*; D018533:Biomass; D004785:Environmental Pollutants / Q000737:chemistry / Q000633:toxicity; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D035683:MicroRNAs / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development
chemical_list: D004785:Environmental Pollutants; D035683:MicroRNAs; D018749:RNA, Plant; D000537:Aluminum Oxide