title: Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge.
abstract: Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5-bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.
authors.affiliation: School of Pharmacy & Pharmaceutical Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK.  sally.freeman@manchester.ac.uk elena.bichenkova@manchester.ac.uk.
authors.forename: Nicholas J W
authors.initials: NJ
authors.lastname: Rattray
authors.forename: Waleed A
authors.initials: WA
authors.lastname: Zalloum
authors.forename: David
authors.initials: D
authors.lastname: Mansell
authors.forename: Joe
authors.initials: J
authors.lastname: Latimer
authors.forename: Carl H
authors.initials: CH
authors.lastname: Schwalbe
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Blake
authors.forename: Elena V
authors.initials: EV
authors.lastname: Bichenkova
authors.forename: Sally
authors.initials: S
authors.lastname: Freeman