title: Plasma separation by centrifugation and subsequent plasma filtration: impact on survival in a pig model of sepsis.
abstract: The impact on survival of a combination of plasma separation by centrifugation and subsequent plasma filtration was tested in a bacterial sepsis model in pigs. In this animal study 19 pigs were included. Groups II and III received an intravenous lethal dose of live Staphylococcus aureus over 1 h; group I received saline (non-septic control--NC). Groups I and II were treated by an extracorporeal circuit consisting of online centrifugation and subsequent plasma filtration (group II: treated group--TG) for 4 h; group III had no specific treatment (septic control, SC). The observation time was 7 days. All animals of group I (NC) and group II (TG) survived, while all animals of group III (SC) died during the observation time. Extracorporeal therapy with online centrifugation and plasma filtration significantly improved survival in a pig model of sepsis. Further studies with this approach are encouraged.
authors.affiliation: Department of Anesthesiology and Intensive Care Medicine, Medical Faculty of the University of Rostock, Rostock, Germany. martin.sauer@uni-rostock.de
authors.forename: Martin
authors.initials: M
authors.lastname: Sauer
authors.forename: Jens
authors.initials: J
authors.lastname: Altrichter
authors.forename: Thomas
authors.initials: T
authors.lastname: Mencke
authors.forename: Sven
authors.initials: S
authors.lastname: Klöhr
authors.forename: Maren
authors.initials: M
authors.lastname: Thomsen
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Kreutzer
authors.forename: Gabriele
authors.initials: G
authors.lastname: Nöldge-Schomburg
authors.forename: Steffen R
authors.initials: SR
authors.lastname: Mitzner
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002498:Centrifugation; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D005112:Extracorporeal Circulation; D005260:Female; D005374:Filtration / Q000379:methods; D007677:Kidney Function Tests; D008111:Liver Function Tests; D010956:Plasmapheresis / Q000379:methods; D011446:Prospective Studies; D018805:Sepsis / Q000097:blood* / Q000382:microbiology / Q000628:therapy*; D013203:Staphylococcal Infections / Q000097:blood* / Q000382:microbiology / Q000628:therapy*; D018709:Statistics, Nonparametric; D013552:Swine
chemical_list: D015415:Biomarkers; D016207:Cytokines