title: Spatial and temporal organization of multi-protein assemblies: achieving sensitive control in information-rich cell-regulatory systems.
abstract: The regulation of cellular processes in living organisms requires signalling systems that have a high signal-to-noise ratio. This is usually achieved by transient, multi-protein complexes that assemble cooperatively. Even in the crowded environment of the cell, such assemblies are unlikely to form by chance, thereby providing a sensitive regulation of cellular processes. Furthermore, selectivity and sensitivity may be achieved by the requirement for concerted folding and binding of previously unfolded components. We illustrate these features by focusing on two essential signalling pathways of eukaryotic cells: first, the monitoring and repair of DNA damage by non-homologous end joining, and second, the mitotic spindle assembly checkpoint, which detects and corrects defective attachments of chromosomes to the kinetochore. We show that multi-protein assemblies moderate the full range of functional complexity and diversity in the two signalling systems. Deciphering the nature of the interactions is central to understanding the mechanisms that control the flow of information in cell signalling and regulation.
authors.affiliation: Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK. victor@cryst.bioc.cam.ac.uk
authors.forename: Victor M
authors.initials: VM
authors.lastname: Bolanos-Garcia
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.forename: Takashi
authors.initials: T
authors.lastname: Ochi
authors.forename: Dimitri Y
authors.initials: DY
authors.lastname: Chirgadze
authors.forename: Bancinyane Lynn
authors.initials: BL
authors.lastname: Sibanda
authors.forename: Tom L
authors.initials: TL
authors.lastname: Blundell
mesh_terms: D000818:Animals; D003460:Crystallization; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D008956:Models, Chemical; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D046912:Multiprotein Complexes