title: Effects of probiotics, probiotic DNA and the CpG oligodeoxynucleotides on ovalbumin-sensitized Brown-Norway rats via TLR9/NF-κB pathway.
abstract: The aim of the study was to investigate the effect of living probiotics, probiotic DNA and the synthetic oligodeoxynucleotides containing CpG motifs (CpG-ODN) on both immune response and intestinal barrier function in ovalbumin-sensitized rat and the underlying mechanisms. Brown-Norway rats were orally sensitized with ovalbumin, and living probiotics, probiotic DNA extraction, synthetic CpG-ODN or non-CpG ODN control was administered. In the living probiotics, probiotic DNA and CpG-ODN groups, the allergic response was significantly inhibited, the Th1/Th2 cytokine balance was shifted away from Th2 side, the percentage of CD4(+) CD25(+high) Treg cells was increased, and the intestinal barrier function was improved. The levels of toll-like receptor (TLR) 9 mRNA and nuclear factor (NF)-κB activity, as well as the IκB-α phosphorylation (p-IκB-α) was significantly increased in these three intervention groups compared with the OVA-positive group, whereas no such effects were found in the non-CpG ODN control group. These data show that the probiotic genomic DNA and the synthetic CpG-ODN was comparable with living probiotics in preventing food allergic response by immune modulation and intestinal barrier function enhancement, and the activation of TLR9/NF-κB signal pathway might be involved in this process.
authors.affiliation: Clinical Nutrition Center, Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhong
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Tang
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Cai
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D004269:DNA, Bacterial / Q000494:pharmacology*; D005260:Female; D005512:Food Hypersensitivity / Q000517:prevention & control; D007155:Immunologic Factors / Q000494:pharmacology*; D016328:NF-kappa B / Q000276:immunology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D010047:Ovalbumin / Q000276:immunology*; D019936:Probiotics / Q000494:pharmacology*; D051381:Rats; D011914:Rats, Inbred BN; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D051217:Toll-Like Receptor 9 / Q000276:immunology*
chemical_list: C408982:CPG-oligonucleotide; D016207:Cytokines; D004269:DNA, Bacterial; D007155:Immunologic Factors; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; C489640:Tlr9 protein, rat; D051217:Toll-Like Receptor 9; D010047:Ovalbumin