title: Immobilization of laccase on polyacrylamide and polyacrylamide - κ - carragennan-based semi-interpenetrating polymer networks.
abstract: In this study, laccase enzyme (L) from Agaricus bisporus was immobilized by entrapment into polyacrylamide (PAAm) and semi-interpenetrating polymer networks (semi-IPNs) prepared with either polyacrylamide/κ-carragennan (0.05g) [PAAm/ κ-car (0.05)] or polyacrylamide/κ-carragennan (0.1 g) [PAAm/ κ-car (0.1)]. The optimum pH was 6.0 for free L, 8.0 for PAAm-L, 8.5 for PAAm/κ-car (0.05)-L, and 9.0 for PAAm/κ-car (0.1)-L. The optimum temperature was determined as 45°C for free L and 60°C for all immobilized laccases. After 27 days of storage at 4°C, free enzyme lost its initial activity whereas immobilized enzymes retained 56 % (-)80% of their initial activities. The immobilized samples were used repeatedly 35 times by retaining 28 %-58 % of their initial activity. K(m(app)) values were calculated as 0.088, 0.139, 0.133, and 0.131 mM and Vmax values were found to be 2.83 x 10(-3), 4.51×10(-3), 4.76×10(-3), and 4.97×10(-3) mM min(-1) for free L and PAAm-L, PAAm/κ-car (0.05)-L, and PAAm/κ-car (0.1)-L, respectively.
authors.affiliation: Department of Chemistry, Kirikkale University, Kirikkale, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Gökgöz
authors.forename: H
authors.initials: H
authors.lastname: Altinok
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000364:Agaricus / Q000201:enzymology*; D002351:Carrageenan / Q000737:chemistry*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry*; D055550:Protein Stability; D013696:Temperature; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D002351:Carrageenan; D004800:Enzymes, Immobilized; D020100:Hydrogels; D042845:Laccase; D000099166:Polyacrylamides