title: Investigation of the nucleotide triphosphate substrate specificity of Homo sapiens UDP-N-acetylgalactosamine pyrophosphorylase (AGX1).
abstract: Nucleotide sugars are essential glycosyl donors for Leloir-type glycosyltransferases. The UDP-N-acetylgalactosamine pyrophosphorylase (UDP-GalNAc PP; AGX1) from Homo sapiens catalyzes the synthesis of UDP-N-acetylgalactosamine from N-acetylgalactosamine 1-phosphate and UTP. In this Letter, we systematically studied nucleotide substrate specificity of AGX1 during its uridyltransfer reaction, and described the capability of AGX1 to catalyze dUTP and dTTP to their corresponding nucleotide sugars for the first time. Furthermore, using such a eukaryotic enzyme, we synthesized dUDP-GalNAc and dTDP-GalNAc in multiple mg scale in vitro efficiently and rapidly.
authors.affiliation: National Glycoengineering Research Center, Jinan, Shandong 250100, People's Republic of China.
authors.forename: Mengyang
authors.initials: M
authors.lastname: Xue
authors.forename: Wanyi
authors.initials: W
authors.lastname: Guan
authors.forename: Yang
authors.initials: Y
authors.lastname: Zou
authors.forename: Junqiang
authors.initials: J
authors.lastname: Fang
authors.forename: Xian-wei
authors.initials: XW
authors.lastname: Liu
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
authors.forename: Fengshan
authors.initials: F
authors.lastname: Wang
mesh_terms: D000116:Acetylgalactosamine / Q000031:analogs & derivatives* / Q000737:chemistry; D055162:Biocatalysis; D004926:Escherichia coli; D005700:Galactosyltransferases / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D013942:Thymine Nucleotides / Q000737:chemistry*; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000138:chemical synthesis*; D014544:Uridine Triphosphate / Q000737:chemistry*
chemical_list: C115144:N-acetylgalactosamine-1-phosphate; D011994:Recombinant Proteins; D013942:Thymine Nucleotides; D014536:Uridine Diphosphate N-Acetylgalactosamine; D005700:Galactosyltransferases; C511281:UDP-GalNAc pyrophosphorylase AGX1, human; D000116:Acetylgalactosamine; C024157:thymidine 5'-triphosphate; D014544:Uridine Triphosphate