title: Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis.
abstract: Locus At5g03555 encodes a nucleobase cation symporter 1 (AtNCS1) in the Arabidopsis genome. Arabidopsis insertion mutants, AtNcs1-1 and AtNcs1-3, were used for in planta toxic nucleobase analog growth studies and radio-labeled nucleobase uptake assays to characterize solute transport specificities. These results correlate with similar growth and uptake studies of AtNCS1 expressed in Saccharomyces cerevisiae. Both in planta and heterologous expression studies in yeast revealed a unique solute transport profile for AtNCS1 in moving adenine, guanine and uracil. This is in stark contrast to the canonical transport profiles determined for the well-characterized S. cerevisiae NCS1 proteins FUR4 (uracil transport) or FCY2 (adenine, guanine, and cytosine transport).
authors.affiliation: Department of Biology, Indiana University-Purdue University Fort Wayne, Fort Wayne, IN 46805, USA. mourad@ipfw.edu
authors.forename: George S
authors.initials: GS
authors.lastname: Mourad
authors.forename: Julie
authors.initials: J
authors.lastname: Tippmann-Crosby
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Hunt
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Gicheru
authors.forename: Kaely
authors.initials: K
authors.lastname: Bade
authors.forename: Tyler A
authors.initials: TA
authors.lastname: Mansfield
authors.forename: Neil P
authors.initials: NP
authors.lastname: Schultes
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001692:Biological Transport; D056426:Genetic Loci / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D033702:Nucleobase Transport Proteins; D011687:Purines / Q000737:chemistry / Q000633:toxicity; D011743:Pyrimidines / Q000737:chemistry / Q000633:toxicity; D016415:Sequence Alignment; D027981:Symporters / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C572959:NCS1 protein, Arabidopsis; D033702:Nucleobase Transport Proteins; D011687:Purines; D011743:Pyrimidines; D027981:Symporters; C030986:pyrimidine; C030985:purine