title: A monosegmented flow-batch system for slow reaction kinetics: spectrophotometric determination of boron in plants.
abstract: This work introduces the monosegmented flow-batch (MSFB) analysis concept. This system combines favourable characteristics of both flow-batch and the monosegmented analysers, allowing use of the flow-batch system for slow reaction kinetics without impairing sensitivity or sampling throughput. The MSFB was evaluated during spectrophotometric determination of boron in plant extracts, which is a method that involves a slow reaction between boron and azomethine-H. All calibration solutions were prepared in-line, and all analytical processes completed by simply changing the operational parameters in the MSFB control software. The limit of detection was estimated at 0.008 mg L(-1). The measurements could be performed at a rate of 120 samples per hour with satisfactory precision. The proposed MSFB was successfully applied to analyse 10 plant samples and the results are in agreement with the reference method at a 95% level of confidence.
authors.affiliation: Universidade Federal da Paraíba, Centro de Ciências Exatas e da Natureza, Departamento de Química, João Pessoa, PB, Brazil.
authors.forename: Inakã S
authors.initials: IS
authors.lastname: Barreto
authors.forename: Stéfani Iury E
authors.initials: SI
authors.lastname: Andrade
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Lima
authors.forename: Edvan C
authors.initials: EC
authors.lastname: Silva
authors.forename: Mário César U
authors.initials: MC
authors.lastname: Araújo
authors.forename: Luciano F
authors.initials: LF
authors.lastname: Almeida
mesh_terms: D001895:Boron / Q000032:analysis*; D002138:Calibration; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D007700:Kinetics; D057230:Limit of Detection; D009282:Naphthalenesulfonates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D012984:Software; D012996:Solutions; D013053:Spectrophotometry; D013882:Thiosemicarbazones / Q000737:chemistry
chemical_list: D009282:Naphthalenesulfonates; D010936:Plant Extracts; D012996:Solutions; D013882:Thiosemicarbazones; C097966:azomethine H; D001895:Boron