title: Administration of vitamin E prevents thymocyte apoptosis in murine sarcoma S180 tumor bearing mice.
abstract: Many reports claimed that thymic involution occurred in tumor bearing host, which led to a reduction of proliferation in T cells and an impaired immune system. In this study, murine sarcoma S180 tumor cells were used to establish tumor model. There was distinct apoptosis in thymus of the S180 tumor bearing host after 3-week tumor inoculation. High level reactive oxygen species generation was detected in thymocytes of S180 tumor bearers. Vitamin E, a potent antioxidant, was oral administrated to S180 tumor bearing mice in order to eliminate redundant ROS in the host and evaluate the effect against thymic apoptosis. Intact thymic structure and less apoptosis of thymocytes demonstrated that partial restoration of thymuses in S180 tumor bearing mice after 3—week VE treatment. Besides, VE treatment normalized ROS level, MDA level and SOD level of S180 tumor bearers. Thus, it could be concluded that thymic involution prevented by VE treatment in S180 tumor bearing mice was mainly due to inhibition of apoptosis in thymus and elimination of ROS over—production in the host.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Institute of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, China. laj@tust.edu.cn
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: A
authors.initials: A
authors.lastname: Liu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000235:genetics; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D041681:NIH 3T3 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013950:Thymus Gland / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013953:Thymus Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D051020:bcl-X Protein / Q000235:genetics
chemical_list: D000975:Antioxidants; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051020:bcl-X Protein; D014810:Vitamin E; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D053148:Caspase 3