title: Antiviral activities and putative identification of compounds in microbial extracts from the Hawaiian coastal waters.
abstract: Marine environments are a rich source of significant bioactive compounds. The Hawaiian archipelago, located in the middle of the Pacific Ocean, hosts diverse microorganisms, including many endemic species. Thirty-eight microbial extracts from Hawaiian coastal waters were evaluated for their antiviral activity against four mammalian viruses including herpes simplex virus type one (HSV-1), vesicular stomatitis virus (VSV), vaccinia virus and poliovirus type one (poliovirus-1) using in vitro cell culture assay. Nine of the 38 microbial crude extracts showed antiviral potencies and three of these nine microbial extracts exhibited significant activity against the enveloped viruses. A secosteroid, 5α(H),17α(H),(20R)-beta-acetoxyergost-8(14)-ene was putatively identified and confirmed to be the active compound in these marine microbial extracts. These results warrant future in-depth tests on the isolation of these active elements in order to explore and validate their antiviral potential as important therapeutic remedies.
authors.affiliation: Department of Public Health Sciences, University of Hawaii at Manoa, East-West Road, Honolulu, HI 96822, USA.|Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Institute of Traditional Chinese Medicine & Natural Products, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tong
authors.affiliation: Center for Marine Microbial Ecology and Diversity, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
authors.forename: Hank
authors.initials: H
authors.lastname: Trapido-Rosenthal
authors.affiliation: Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, East-West Road, Honolulu, HI 96822, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Institute of Traditional Chinese Medicine & Natural Products, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Youwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, East-West Road, Honolulu, HI 96822, USA.
authors.forename: Qing X
authors.identifier: 0000-0003-4589-2869
authors.initials: QX
authors.lastname: Li
authors.affiliation: Department of Public Health Sciences, University of Hawaii at Manoa, East-West Road, Honolulu, HI 96822, USA.
authors.forename: Yuanan
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D059001:Aquatic Organisms; D001419:Bacteria / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D002849:Chromatography, Gas; D006254:Hawaii; D018259:Herpesvirus 1, Human / Q000187:drug effects; D006801:Humans; D013058:Mass Spectrometry; D010140:Pacific Ocean; D011161:Porifera / Q000737:chemistry; D012623:Seawater / Q000382:microbiology*; D014709:Vero Cells; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents