title: An LC-MS/MS method for determination of jujuboside A in rat plasma and its application to pharmacokinetic studies.
abstract: A novel, simple, and sensitive method for the determination of jujuboside A in rat plasma using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed. Following solid-phase extraction, measurement of jujuboside A was performed by negative ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The limit of detection was 1.25 ng/mL, and the lower limit of quantification was 5 ng/mL in rat plasma. Good linearity was obtained over the range of 6.25-500 ng/mL, and the correlation coefficient was better than 0.998. The intra- and inter-day precisions ranged 4.4-7.5% and 2.9-10.7%, respectively. The accuracy derived from QC samples ranged 3.2-7.8% and 2.2-3.5%, respectively. The recovery ranged from 72.9 to 75.1% and the matrix effect from 96.7 to 105.3%. The analyte was stable under various conditions (at room temperature, during freeze-thaw, in the autosampler and under deep-freeze conditions). The developed method was successfully applied to the pharmacokinetic study in rats.
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Guangzhou 510405, Guangdong Province, China.
authors.forename: Changhui
authors.initials: C
authors.lastname: Liu
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Li
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Zhong
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Huang
authors.forename: Xia
authors.initials: X
authors.lastname: Zheng
authors.forename: Neng
authors.initials: N
authors.lastname: Li
authors.forename: Shaoling
authors.initials: S
authors.lastname: He
authors.forename: Suiqing
authors.initials: S
authors.lastname: Mi
authors.forename: Ningsheng
authors.initials: N
authors.lastname: Wang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000097:blood* / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*; D031957:Ziziphus / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; C087808:jujuboside A