title: H4K16 acetylation affects recombination and ncRNA transcription at rDNA in Saccharomyces cerevisiae.
abstract: Transcription-associated recombination is an important process involved in several aspects of cell physiology. In the ribosomal DNA (rDNA) of Saccharomyces cerevisiae, RNA polymerase II transcription-dependent recombination has been demonstrated among the repeated units. In this study, we investigate the mechanisms controlling this process at the chromatin level. On the basis of a small biased screening, we found that mutants of histone deacetylases and chromatin architectural proteins alter both the amount of Pol II-dependent noncoding transcripts and recombination products at rDNA in a coordinated manner. Of interest, chromatin immunoprecipitation analyses in these mutants revealed a corresponding variation of the histone H4 acetylation along the rDNA repeat, particularly at Lys-16. Here we provide evidence that a single, rapid, and reversible posttranslational modification-the acetylation of the H4K16 residue-is involved in the coordination of transcription and recombination at rDNA.
authors.affiliation: Dipartimento di Biologia e Biotecnologie, Università di Roma La Sapienza, 00185 Rome, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Cesarini
authors.forename: Anna
authors.initials: A
authors.lastname: D'Alfonso
authors.forename: Giorgio
authors.initials: G
authors.lastname: Camilloni
mesh_terms: D000107:Acetylation; D002843:Chromatin / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D024001:HMGN Proteins / Q000235:genetics; D006657:Histones / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism*; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000096:biosynthesis / Q000235:genetics*; D011995:Recombination, Genetic; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000096:biosynthesis / Q000235:genetics; D056565:Sirtuin 2 / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D024001:HMGN Proteins; D006657:Histones; C086471:NHP6A protein, S cerevisiae; C086472:NHP6B protein, S cerevisiae; D012331:RNA, Fungal; D022661:RNA, Untranslated; D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D012319:RNA Polymerase II; C087778:SIR2 protein, S cerevisiae; D056565:Sirtuin 2