title: Enhanced osteoblast differentiation on scaffolds coated with TiO2 compared to SiO2 and CaP coatings.
abstract: The aim was to compare the protein release from normal human osteoblasts (NHO) cultured on scaffolds with similar morphology but different coatings. Different ceramic coatings; TiO(2), SiO(2) and calcium phosphate (CaP); Ca(9)HPO(4)(PO(4))(5)OH, were applied to porous TiO(2) scaffolds prepared by polymer sponge replication. NHO were cultured on scaffolds in triplicates. The concentration of cytokines and Ca(2+), and alkaline phosphatase (ALP) activity in the cell media was quantified. The secretion of osteopontin, osteoprotegerin, vascular endothelial growth factor and interleukin-6 was higher from NHO on TiO(2) compared to SiO(2) and CaP. The secretion from cells on the three scaffolds was, however, either similar or lower than the control cells cultured on plastic. The Ca(2+) concentration was higher in cell media on CaP the first week, and no difference in ALP activity was observed. TiO(2) coating induced a higher secretion of factors indicating enhanced osteoblast differentiation as compared to CaP and SiO(2).
authors.affiliation: Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, P.O. Box 1109, Blindern, 0317, Oslo, Norway. anderver@odont.uio.no
authors.forename: Anders
authors.initials: A
authors.lastname: Verket
authors.forename: Hanna
authors.initials: H
authors.lastname: Tiainen
authors.forename: Håvard J
authors.initials: HJ
authors.lastname: Haugen
authors.forename: S Petter
authors.initials: SP
authors.lastname: Lyngstadaas
authors.forename: Ola
authors.initials: O
authors.lastname: Nilsen
authors.forename: Janne E
authors.initials: JE
authors.lastname: Reseland
mesh_terms: D002130:Calcium Phosphates / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D012822:Silicon Dioxide / Q000737:chemistry*; D054457:Tissue Scaffolds; D014025:Titanium / Q000737:chemistry*
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D012822:Silicon Dioxide; C020243:calcium phosphate; D014025:Titanium