title: 2b-RAD: a simple and flexible method for genome-wide genotyping.
abstract: We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput genotyping as required for linkage mapping and profiling genetic variation in natural populations.
authors.affiliation: School of Biological Sciences, University of Texas at Austin, Austin, Texas, USA. swang@ouc.edu.cn
authors.forename: Shi
authors.initials: S
authors.lastname: Wang
authors.forename: Eli
authors.initials: E
authors.lastname: Meyer
authors.forename: John K
authors.initials: JK
authors.lastname: McKay
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Matz
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D004247:DNA / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D016678:Genome / Q000235:genetics*; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific