title: Effects of rutin on the expression of PPARγ in skeletal muscles of db/db mice.
abstract: The effect of rutin (RT) on the expression of PPARγ in the skeletal muscle of db/db mice has been investigated. Thirty db/db mice were randomized into 5 equal groups: model control group (MCG), positive control group 30 mg/kg pioglitazone hydrochloride (PCG), low-dose rutin group 50 mg/kg (LRT), middle-dose rutin group 100 mg/kg (MRT), high-dose rutin group 200 mg/kg (HRT), and 6 other db/m mice were used as the normal control (NC). The expression of PPARγ mRNA and protein in the skeletal muscle was determined by real-time quantitative PCR and Western Blot, respectively. Treatment with HRT resulted in significant decreases in the plasma levels of glucose and lipids (p < 0.001). Compared to the MCG, the expression of PPARγ mRNA and protein of the skeletal muscle was significantly increased in the HRT and PCG groups (p < 0.001 and p < 0.05, respectively). Rutin can increase the expression of PPARγ in skeletal muscles of db/db mice.
authors.affiliation: Life Science College of the Zhejiang Chinese Medical University, Hangzhou, Zhejiang Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cai
authors.forename: Chunlei
authors.initials: C
authors.lastname: Fan
authors.forename: Jin
authors.initials: J
authors.lastname: Yan
authors.forename: Nan
authors.initials: N
authors.lastname: Tian
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D051379:Mice; D008820:Mice, Obese; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism*; D000077205:Pioglitazone; D012431:Rutin / Q000008:administration & dosage / Q000494:pharmacology*; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D001786:Blood Glucose; D008055:Lipids; D047495:PPAR gamma; D045162:Thiazolidinediones; D012431:Rutin; D000077205:Pioglitazone