title: Crystallin distribution patterns in Litoria infrafrenata and Phyllomedusa sauvagei lenses.
abstract: The eye lens remains transparent because of soluble lens proteins known as crystallins. For years γ-crystallins have been known as the main lens proteins in lower vertebrates such as fish and amphibians. The unique growth features of the lens render it an ideal structure to study ageing; few studies have examined such changes in anuran lenses. This study aimed to investigate protein distribution patterns in Litoria infrafrenata and Phyllomedusa sauvagei species. Lenses were fractionated into concentric layers by controlled dissolution. Water-soluble proteins were separated into high (HMW), middle (MMW) and low molecular weight (LMW) fractions by size-exclusion HPLC and constituents of each protein class revealed by 1DE and 2DE. Spots were selected from 2DE gels on the basis of known ranges of subunit molecular weights and pH ranges and were identified by MALDI-TOF/TOF MS following trypsin digestion. Comparable lens distribution patterns were found for each species studied. Common crystallins were detected in both species; the most prominent of these was γ-crystallin. Towards the lens centre, there was a decrease in α- and β-crystallin proportions and an increase in γ-crystallins. Subunits representing taxon-specific crystallins demonstrating strong sequence homology with ζ-crystallin/quinone oxidoreductase were found in both L. infrafrenata and P. sauvagei lenses. Further work is needed to determine which amphibians have taxon-specific crystallins, their evolutionary origins, and their function.
authors.affiliation: School of Biomedical Sciences, University of Ulster, Coleraine, UK.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Keenan
authors.forename: Gwen
authors.initials: G
authors.lastname: Manning
authors.forename: Giuliano
authors.initials: G
authors.lastname: Elia
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Dunn
authors.forename: David F
authors.initials: DF
authors.lastname: Orr
authors.forename: Barbara K
authors.initials: BK
authors.lastname: Pierscionek
mesh_terms: D000818:Animals; D001001:Anura; D003459:Crystallins / Q000032:analysis*; D005590:Fractional Precipitation; D007908:Lens, Crystalline / Q000737:chemistry*; D013058:Mass Spectrometry; D008970:Molecular Weight; D038201:alpha-Crystallins / Q000032:analysis; D038204:beta-Crystallins / Q000032:analysis; D038222:gamma-Crystallins / Q000032:analysis; D038226:zeta-Crystallins / Q000032:analysis
chemical_list: D003459:Crystallins; D038201:alpha-Crystallins; D038204:beta-Crystallins; D038222:gamma-Crystallins; D038226:zeta-Crystallins