title: Different from renal artery only clamping, artery and vein clamping causes a significant reduction in number of rat glomeruli during warm ischemia.
abstract: OBJECTIVE
  To evaluate glomerular injury in the rat model during renal warm ischemia (WI), comparing artery and vein (AV) clamping with artery only (AO) clamping.
  
  
  METHODS
  Twenty-four adult male rats underwent 60 minutes of renal WI in the left kidney. The animals were divided into three groups: AV clamping, AO clamping, and Sham surgery. After 30 days, the animals were euthanized, and both kidneys were processed for paraffin embedding and stained with hematoxylin and eosin. Glomerular volume density (Vv[glom]), mean glomerular volume (MGV), and number of glomeruli per mm(3) (Nv[glom]) were evaluated in the renal cortex.
  
  
  RESULTS
  The Vv[glom] was reduced in the left kidney (ischemic) when compared with the right kidney in both AV and AO groups by 11.1% and 35.4%, respectively; however, the difference was significant only in the AV group. The Nv[glom] was reduced in the left kidney when compared with the right kidney in both AV and AO groups by 11.6% and 31.4%, respectively; nevertheless, the difference was significant only in the AV group. The MGV of left and right kidneys was the same in both Sham and AO groups and was diminished by 6.7% in the AV group-not significant.
  
  
  CONCLUSIONS
  AV clamping causes a significant decrease in the number of glomeruli in the rat model, while AO clamping reduces the glomerular number, but not significantly. To minimize renal injury, AO clamping may be preferred over AV clamping when WI is necessary in patients with previously compromised renal function.
authors.affiliation: Urogenital Research Unit, State University of Rio de Janeiro, Rua Comendador Queiroz 37/902, Rio de Janeiro, RJ, Brazil.
authors.forename: Hélio J S
authors.initials: HJ
authors.lastname: Bagetti-Filho
authors.forename: Francisco J B
authors.initials: FJ
authors.lastname: Sampaio
authors.forename: Ruy G
authors.initials: RG
authors.lastname: Marques
authors.forename: Marco A
authors.initials: MA
authors.lastname: Pereira-Sampaio
mesh_terms: D000818:Animals; D003250:Constriction; D004195:Disease Models, Animal; D005919:Glomerular Filtration Rate; D007511:Ischemia / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D007668:Kidney / Q000098:blood supply / Q000503:physiopathology / Q000601:surgery; D007674:Kidney Diseases / Q000503:physiopathology; D007677:Kidney Function Tests; D007678:Kidney Glomerulus / Q000098:blood supply* / Q000503:physiopathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012077:Renal Artery / Q000601:surgery*; D012082:Renal Veins / Q000601:surgery*; D052096:Warm Ischemia / Q000379:methods*