title: Self-assembled mPEG-PCL-g-PEI micelles for simultaneous codelivery of chemotherapeutic drugs and DNA: synthesis and characterization in vitro.
abstract: BACKGROUND
  In this paper, a series of amphiphilic triblock copolymers based on polyethylene glycol-poly ɛ-caprolactone-polyethylenimine (mPEG-PCL-g-PEI) were successfully synthesized, and their application for codelivery of chemotherapeutic drugs and DNA simultaneously was investigated.
  
  
  RESULTS
  These copolymers could self-assemble into micelles with positive charges. The size and zeta potential of the micelles was measured, and the results indicate that temperature had a large effect on the micelles obtained. In vitro gene transfection evaluation in cancer cells indicated that the self-assembled micelles could serve as potential gene delivery vectors. In addition, hydrophobic drug entrapment efficiency and codelivery with the gene was also studied in vitro. The self-assembled micelles could load doxorubicin efficiently and increase cellular uptake in vitro, while maintaining high gene transfection efficiency.
  
  
  CONCLUSIONS
  The triblock copolymer mPEG-PCL-g-PEI could be a novel vector for codelivery of drug and gene therapy.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Shi
authors.forename: Xuechen
authors.initials: X
authors.lastname: Zhu
authors.forename: Qingfa
authors.initials: Q
authors.lastname: Guo
authors.forename: Yingjing
authors.initials: Y
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Zuo
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Qian
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002460:Cell Line; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004317:Doxorubicin / Q000008:administration & dosage; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D015316:Genetic Therapy; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008823:Micelles; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D053769:Nanocapsules / Q000737:chemistry / Q000648:ultrastructure; D050997:Nanomedicine; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008823:Micelles; D053769:Nanocapsules; D011091:Polyesters; C439611:methoxy poly(ethylene glycol-co-epsilon-caprolactone); D011092:Polyethylene Glycols; D004317:Doxorubicin; D011094:Polyethyleneimine; D004247:DNA