title: Genetic influences on vitamin D status and forearm fracture risk in African American children.
abstract: We sought to investigate the relationship between newly identified genetic variants and vitamin D levels and fracture risk in healthy African American (black) children. This case-control study included children of both sexes, ages 5 to 9 years, with and without forearm fractures. Serum 25-hydroxy vitamin D levels, bone mineral density, body mass index, and calcium/vitamin D intake were measured in 130 individuals (n = 60 cases and n = 70 controls). The 5 variants tested were located in the GC gene (rs2282679), in the NADSYN1 gene (rs12785878 and rs3829251), and in the promoter region of the CYP2R1 gene (rs2060793 and rs104741657). Associations between single nucleotide polymorphisms (SNPs) and vitamin D levels were tested using an analysis of covariance. Associations between SNPs and fracture status were tested using logistic regression. The GC gene variant was associated with vitamin D levels (P = 0.038). None of the SNPs were associated with fracture status in young blacks. These results suggest that the variants tested, which are associated with circulating vitamin D levels in whites, are not associated with fracture status in healthy black children. Additional research is required to discover the genetics of fracture risk in blacks.
authors.affiliation: Division of Emergency Medicine, Children's National Medical Center, Washington, DC 20010, USA. lryan@childrensnational.org
authors.forename: Leticia Manning
authors.initials: LM
authors.lastname: Ryan
authors.forename: James M
authors.initials: JM
authors.lastname: Chamberlain
authors.forename: Steven A
authors.initials: SA
authors.lastname: Singer
authors.forename: Rachel
authors.initials: R
authors.lastname: Wood
authors.forename: Laura L
authors.initials: LL
authors.lastname: Tosi
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Freishtat
authors.forename: Heather
authors.initials: H
authors.lastname: Gordish-Dressman
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Teach
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Devaney
mesh_terms: D015502:Absorptiometry, Photon; D001741:Black or African American / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D003710:Demography; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011885:Radius Fractures / Q000097:blood* / Q000000981:diagnostic imaging / Q000235:genetics*; D014458:Ulna Fractures / Q000097:blood* / Q000000981:diagnostic imaging / Q000235:genetics*; D014807:Vitamin D / Q000097:blood*
chemical_list: D014807:Vitamin D