title: [Antiviral activity of recombinant interferon-alpha-2b in combination with certain antioxidant].
abstract: In vitro activity of interferon-alpha-2b in combination with various antioxidants against the influenza virus and Herpes simplex was studied. The standard strains and a clinical strain of Herpes simplex isolated from a patient with resistance to acyclovir were used. The in vitro studie showed that antioxidants, such as alpho-tocoferol acetate (vitamin E), Unithiol and ascorbic acid had a significant antiinfluenzae and antiherpetic action on the influenza virus A/H5N1 and Herpes simplex variants. They protected up to 100% of the cell monolayer from the virus cytopathic effect. The taurin solutions had no antiviral activity irrespective of the infection dose. Combinations of interferon-alpha-2b with alpha-tocopherol acetate (vitamin E), Unithiol or ascorbic acid showed a significant synergistic effect: the antiviral activity of interferon increased several times. The antiinfluenza activity of interferon-a-2b in the presence of various concentrations of taurin did not change.
authors.forename: A N
authors.initials: AN
authors.lastname: Vasil'ev
authors.forename: P G
authors.initials: PG
authors.lastname: Deriabin
authors.forename: G A
authors.initials: GA
authors.lastname: Galegov
mesh_terms: D000212:Acyclovir / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001205:Ascorbic Acid / Q000494:pharmacology / Q000627:therapeutic use; D002460:Cell Line; D002522:Chlorocebus aethiops; D004357:Drug Synergism; D006561:Herpes Simplex / Q000188:drug therapy; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000187:drug effects*; D007251:Influenza, Human / Q000188:drug therapy; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology* / Q000627:therapeutic use; D007668:Kidney / Q000166:cytology; D008168:Lung / Q000166:cytology; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D018139:Simplexvirus / Q000187:drug effects* / Q000302:isolation & purification; D013552:Swine; D013654:Taurine / Q000494:pharmacology / Q000627:therapeutic use; D014494:Unithiol / Q000494:pharmacology / Q000627:therapeutic use; D014709:Vero Cells; D024502:alpha-Tocopherol / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D000998:Antiviral Agents; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D013654:Taurine; D014494:Unithiol; D024502:alpha-Tocopherol; D001205:Ascorbic Acid; D000212:Acyclovir