title: Effect of interferon-γ on the fusion of mononuclear osteoclasts into bone-resorbing osteoclasts.
abstract: Osteoclasts are multinucleated cells that are formed by the fusion of pre-fusion osteoclasts (pOCs). The fusion of pOCs is known to be important for osteoclastic bone resorption. Here, we examined the effect of IFN-γ on the fusion of pOCs. IFN-γ greatly increased the fusion of pOCs in a dose-dependent manner. Furthermore, IFN-γ induced pOC fusion even in hydroxyapatite-coated plates used as a substitute for bone. The resorption area of pOCs stimulated with IFN-γ was significantly higher than that of the control cells. IFN-γ induced the expression of dendritic cell-specific transmembrane protein (DC-STAMP), which is responsible for the fusion of pOCs. IFN-γ enhanced DC-STAMP expression in a dose-dependent manner. The mRNA expression of c-Fos and nuclear factor of activated T cells (NFAT) c1 was enhanced in the pOCs treated with IFN-γ. Taken together, these results provide a new insight into the novel role of IFN-γ on the fusion of pOCs.
authors.affiliation: Department of Orthopaedic Surgery, Division Rheumatology, Wonkwang University School of Medicine, Iksan 570-749, Korea.
authors.forename: Jeung Woo
authors.initials: JW
authors.lastname: Kim
authors.forename: Myeung Su
authors.initials: MS
authors.lastname: Lee
authors.forename: Chang Hoon
authors.initials: CH
authors.lastname: Lee
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Soo Uk
authors.initials: SU
authors.lastname: Chae
authors.forename: Han Bok
authors.initials: HB
authors.lastname: Kwak
authors.forename: Jaemin
authors.initials: J
authors.lastname: Oh
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002459:Cell Fusion; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D015870:Gene Expression / Q000187:drug effects; D015726:Giant Cells / Q000187:drug effects* / Q000473:pathology* / Q000502:physiology; D007371:Interferon-gamma / Q000494:pharmacology* / Q000502:physiology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010010:Osteoclasts / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D016207:Cytokines; C025940:bone resorption factor; D007371:Interferon-gamma