title: Clinical, haematological and biochemical responses of sheep undergoing autologous blood transfusion.
abstract: BACKGROUND
  This study aimed to evaluate the clinical, haematological and biochemical responses to autologous blood transfusion and the feasibility of this practice in sheep. Thus, we used eight male, 8 months old sheep, weighing on average 30 kg, from which 15 mL/kg of whole blood was collected and stored in CPDA-1 bags. Blood samples were refrigerated for 8 days and subsequently re-infused. The clinical, haematological and biochemical parameters were evaluated before blood collection and reinfusion, after 10 minutes of collection and reinfusion, after 3, 6, 12, 24, 48, 96 and 192 hours after collection and reinfusion.
  
  
  RESULTS
  With respect to clinical parameters, we observed a decrease in heart rate after 24, 48 and 196 hours from reinfusion compared to basal values (p < 0.05). Haematological variables including globular volume and erythrocyte counts showed a significant decrease (p < 0.01) at all time points after collection and increased (p < 0.01) at all time points after reinfusion. There was a significant increase in total protein and calcium at all time points after reinfusion (p < 0.05).
  
  
  CONCLUSIONS
  Autologous transfusion in sheep slightly altered the physiological, biochemical and haematological responses of sheep, indicating that the technique proposed is safe and can be applied in the clinical practice of this species. The 8 d period was not sufficient for complete recovery of the haematological parameters after blood collection.
authors.affiliation: Federal Rural University of Semi-Arid, Av. Francisco Mota, 572, Bairro Costa e Silva, CEP: 59.625-900, Mossoró, RN, Brazil.
authors.forename: Rejane Santos
authors.initials: RS
authors.lastname: Sousa
authors.forename: Dowglish Ferreira
authors.initials: DF
authors.lastname: Chaves
authors.forename: Raimundo Alves
authors.initials: RA
authors.lastname: Barrêto-Júnior
authors.forename: Isadora Karolina Freitas
authors.initials: IK
authors.lastname: Sousa
authors.forename: Herbert Sousa
authors.initials: HS
authors.lastname: Soares
authors.forename: Isabella Oliveira
authors.initials: IO
authors.lastname: Barros
authors.forename: Antonio Humberto Hamad
authors.initials: AH
authors.lastname: Minervino
authors.forename: Enrico Lippi
authors.initials: EL
authors.lastname: Ortolani
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D001804:Blood Transfusion, Autologous / Q000662:veterinary*; D002118:Calcium / Q000097:blood*; D004906:Erythrocyte Count / Q000662:veterinary*; D006339:Heart Rate / Q000502:physiology; D008297:Male; D012756:Sheep; D013997:Time Factors
chemical_list: D001798:Blood Proteins; D002118:Calcium