title: Nested PCR-positive tubercular ampiginous choroiditis: a case report.
abstract: OBJECTIVE
  To report on ampiginous choroiditis of tubercular etiology with nPCR positivity for M. tb in aqueous sample and also to stress upon the usefulness of nPCR to consolidate the etiological diagnosis.
  
  
  METHODS
  Case report of a patient presenting with active ampiginous choroiditis with no evidence of systemic TB but had hilar and para-tracheal lymphadenopathy on CT chest. QuantiFERON TB gold test was positive The patient responded inadequately to systemic anti-tubercular treatment and oral steroids. Nested PCR for M.tb was done on aqueous sample was positive for MPB64 gene of M.tb. Anti-TB treatment was continued inspite of initial poor response to which the patient responded and his choroiditis regressed.
  
  
  RESULTS
  Ocular tuberculosis forms the essential differential diagnosis of any chronic or recurrent choroiditis, especially in high endemic areas. The presumed tubercular etiology can be substantiated using highly sensitive and specific tests such as nested PCR on intraocular fluid.
authors.forename: Sagar Sudhakar
authors.initials: SS
authors.lastname: Bhuibhar
authors.forename: Jyotirmay
authors.initials: J
authors.lastname: Biswas
mesh_terms: D000328:Adult; D000995:Antitubercular Agents / Q000627:therapeutic use; D001082:Aqueous Humor / Q000382:microbiology; D002833:Choroiditis / Q000175:diagnosis / Q000382:microbiology*; D004359:Drug Therapy, Combination; D006801:Humans; D055985:Latent Tuberculosis / Q000175:diagnosis / Q000188:drug therapy; D008297:Male; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016896:Treatment Outcome; D014392:Tuberculosis, Ocular / Q000175:diagnosis*
chemical_list: D000995:Antitubercular Agents