title: Isolation and purification of a novel deca-antifungal peptide from potato (Solanum tuberosum L. cv. Jopung) against Candida albicans.
abstract: In a previous study, an antifungal protein, AFP-J, was purified from tubers of the potato (Solanum tuberosum cv. L Jopung) and by gel filtration and HPLC. In this study, the functional peptide was characterized by partial acid digestion using HCl and HPLC. We obtained three peaks from the AFP-J, the first and third peaks were not active in the tested fungal strain. However, the second peak, which was named Potide-J, was active (MIC; 6.25 μg/mL) against Candida albicans. The amino acid sequences were analyzed by automated Edman degradation, and the amino acid sequence of Potide-J was determined to be Ala-Val-Cys-Glu-Asn-Asp-Leu-Asn-Cys-Cys. Mass spectrometry showed that its molecular mass was 1083.1 Da. Finally, we confirmed that a disulfide bond was present between Cys(3) and Cys(9) or Cys(10). Using this structure, Potide-J was synthesized via solid-phase methods. In these experiments, only the linear sequence was shown to display strong activity against Candida albicans. These results suggest that Potide-J may be an excellent candidate compound for the development of commercially applicable antibiotic agents.
authors.affiliation: Research Center for Proteineous Materials, Chosun University, Gwangju 501-759, Korea.
authors.forename: Jong-Kook
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Research Center for Proteineous Materials, Chosun University, Gwangju 501-759, Korea.
authors.forename: Ramamourthy
authors.initials: R
authors.lastname: Gopal
authors.affiliation: Department of Bioinformatics, Kongju National University, Kongju, South Korea.
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Seo
authors.affiliation: Department of Biotechnology, Chosun University, Gwangju 501-759, Korea.
authors.forename: Hyeonsook
authors.initials: H
authors.lastname: Cheong
authors.affiliation: Research Center for Proteineous Materials, Chosun University, Gwangju 501-759, Korea.|Department of Biotechnology, Chosun University, Gwangju 501-759, Korea.
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000494:pharmacology; D010940:Plant Proteins / Q000494:pharmacology*; D035281:Plant Tubers / Q000378:metabolism; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D020539:Sequence Analysis, Protein; D011198:Solanum tuberosum / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D010936:Plant Extracts; D010940:Plant Proteins; D011480:Protease Inhibitors