title: Patterning of antibodies using flexographic printing.
abstract: Antibodies were patterned onto flexible plastic films using the flexographic printing process. An ink formulation was developed using high molecular weight polyvinyl alcohol in carbonate-bicarbonate buffer. In order to aid both antibody adhesion and the quality of definition in the printed features, a nitrocellulose coating was developed that was capable of being discretely patterned, thus increasing the signal-to-noise ratio of an antibody array. Printing antibody features such as dots, squares, text, and fine lines were reproduced effectively. Furthermore, this process could be easily adapted for printing of other biological materials, including, but not limited to, enzymes, DNA, proteins, aptamers, and cells.
authors.affiliation: Welsh Centre for Printing and Coating, College of Engineering, Swansea University, Singleton Park, Swansea, SA2 8PP, UK. c.o.phillips@swansea.ac.uk
authors.forename: Christopher O
authors.initials: CO
authors.lastname: Phillips
authors.forename: Sridhar
authors.initials: S
authors.lastname: Govindarajan
authors.forename: Simon M
authors.initials: SM
authors.lastname: Hamblyn
authors.forename: R Steven
authors.initials: RS
authors.lastname: Conlan
authors.forename: David T
authors.initials: DT
authors.lastname: Gethin
authors.forename: Tim C
authors.initials: TC
authors.lastname: Claypole
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000378:metabolism; D003101:Collodion / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry; D009195:Peroxidase / Q000378:metabolism; D011327:Printing / Q000379:methods*; D012212:Rheology
chemical_list: D055910:Antibodies, Immobilized; D004396:Coloring Agents; D003101:Collodion; D009195:Peroxidase