title: CD4 costimulation is not required in a novel LPS-enhanced model of myasthenia gravis.
abstract: The potential of lipopolysaccharide (LPS) to induce antigen-specific B cell responses to acetylcholine receptor (AChR) in myasthenia gravis (MG) was evaluated in wild type (WT) and CD4-/- C57BL/6 mice. The WT mice immunized with AChR in LPS developed an MG-like disease (LPS-EAMG) similar to that induced by immunization with AChR in complete Freund's adjuvant (CFA-EAMG). CD4-/- mice were resistant to CFA-EAMG but susceptible to LPS-EAMG. LPS abrogated EAMG resistance in CD4-/- mice by increasing high-affinity anti-AChR IgG2b in sera and enhancing immune complex deposition in muscle.
authors.affiliation: Department of Microbiology and Immunology, The University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-1070, United States
authors.forename: Windy
authors.initials: W
authors.lastname: Allman
authors.forename: Huibin
authors.initials: H
authors.lastname: Qi
authors.forename: Shamsher S
authors.initials: SS
authors.lastname: Saini
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Erdem
authors.initials: E
authors.lastname: Tuzun
authors.forename: Premkumar
authors.initials: P
authors.lastname: Christadoss
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant / Q000276:immunology / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008856:Microscopy, Fluorescence; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000276:immunology*; D011950:Receptors, Cholinergic / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D001324:Autoantigens; D008070:Lipopolysaccharides; D011950:Receptors, Cholinergic; D005620:Freund's Adjuvant