title: Response surface methodology to optimize enzymatic preparation of Deapio-Platycodin D and Platycodin D from Radix Platycodi.
abstract: In the present work, we reported the enzymatic preparation of deapio-platycodin D (dPD) and platycodin D (PD) optimized by response surface methodology (RSM) from Radix Platycodi. During investigation of the hydrolysis of crude platycosides by various glycoside hydrolases, snailase showed a strong ability to transform deapio-platycoside E (dPE) and platycoside E (PE) into dPD and PD with 100% conversion. RSM was used to optimize the effects of the reaction temperature (35-45 °C), enzyme load (5-20%), and reaction time (4-24 h) on the conversion process. Validation of the RSM model was verified by the good agreement between the experimental and the predicted values of dPD and PD conversion yield. The optimum preparation conditions were as follows: temperature, 43 °C; enzyme load, 15%; reaction time, 22 h. The biotransformation pathways were dPE→dPD3→dPD and PE→PD3→PD, respectively. The determined method may be highly applicable for the enzymatic preparation of dPD and PD for medicinal purposes and also for commercial use.
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Wei
authors.identifier: 0000-0002-2988-4298
authors.initials: W
authors.lastname: Li
authors.affiliation: The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College, Nanning 530011, China.
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Zhao
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yi-Nan
authors.initials: YN
authors.lastname: Zheng
authors.affiliation: The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College, Nanning 530011, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Liang
authors.affiliation: China-Japan Union Hospital, Jilin University, Changchun 130033, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D006868:Hydrolysis; D008516:Medicine, Chinese Traditional; D015233:Models, Statistical; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000138:chemical synthesis* / Q000737:chemistry; D018517:Plant Roots / Q000378:metabolism; D031238:Platycodon / Q000378:metabolism*; D012503:Saponins / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D014315:Triterpenes / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C000593592:deapi-platycodin D; C121315:platycoside E; D009828:Oleanolic Acid; C108953:platycodin D