title: [Expression of OCT4 protein in bladder cancer and its clinicopathological implications].
abstract: OBJECTIVE
  To study the expression of OCT4 protein in bladder cancer and its correlation to the clinicopathologic features and prognosis of bladder cancer.
  
  
  METHODS
  OCT4 mRNA and protein expression was detected in 5 bladder cancer cell lines (RT-4, Tcc-Sup, KK47, T24, and 5637) and 1 normal bladder cell lines by real-time PCR and Western blotting, respectively. Immunohistochemical analysis was used to detect the expression of OCT4 protein in 46 bladder cancer samples.
  
  
  RESULTS
  All the 5 bladder cancer cell lines expressed detectable levels of OCT4 mRNA and proteins, whereas the normal bladder cell line SV-HUC-1 was negative for OCT4 expression. The clinical bladder cancer tissues showed a high positivity rate of OCT4 expression (76.1%), which was not detected in normal bladder tissues. Specific OCT-4 signals were localized mainly in the nuclei of the cancer cells. The expression rate of OCT4 protein was significantly higher in bladder cancer tissue than in normal bladder epithelium (P<0.05), and showed a positive correlation to the grade of tumor differentiation and metastasis (P<0.05) but not to the patients' age, gender or TNM stage.
  
  
  CONCLUSIONS
  OCT4 protein expression is associated with tumor differentiation and metastasis in bladder cancer and may play an important role in the early diagnosis and prognostic evaluation of bladder cancer.
authors.affiliation: Department of Urinary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China. jnzhaopeng@sina.com
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Zhao
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Liu
authors.forename: Kai
authors.initials: K
authors.lastname: Xu
authors.forename: Shaobo
authors.initials: S
authors.lastname: Zheng
authors.forename: Hulin
authors.initials: H
authors.lastname: Li
authors.forename: Yawen
authors.initials: Y
authors.lastname: Xu
authors.forename: Abai
authors.initials: A
authors.lastname: Xu
authors.forename: Bingkun
authors.initials: B
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D011379:Prognosis; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology*
chemical_list: D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human