title: Distribution of mosquitoes and mosquito-borne viruses along the China-Myanmar border in Yunnan Province.
abstract: A total of 54,673 mosquitoes were collected at 11 sites located near the  China-Myanmar border in the western part of Yunnan Province during July and August 2007.  There were 29 species in 4 genera identified from the collections, including 12 species of  Culex, 12 species of Anopheles, 3 species of Aedes, and 2 species of Armigeres. Culex  tritaeniorhynchus Giles (67.9%, 37,119/54,673) and Anopheles sinensis Wiedemann (25.9%,  14,170/54,673) were the most abundant species in this investigation. Virus was isolated using  BHK-21 and C6/36 cells from 22 of 510 mosquito pools. Isolates included Japanese encephalitis  virus (JEV) and Getah virus (GETV), which were identified by serological and molecular methods.  Twenty JEV strains were isolated from Cx. tritaeniorhynchus (15 isolates), An. sinensis (3  isolates), and Armigeres subalbatus Coquillett (2 isolates), and 2 GETV strains were isolated  from Culex pseudovishnui Colless and Cx. tritaeniorhynchus. This study suggests that Ar.  subalbatus is a potentially important local vector because of the high JEV infection ratio  found in this species. Enzootic JEV transmission persists in this area and therefore,  surveillance for human disease caused by JEV and GETV should be conducted in the region.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.forename: Shihong
authors.initials: S
authors.lastname: Fu
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhang
authors.forename: Minghua
authors.initials: M
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Jinglin
authors.initials: J
authors.lastname: Wang
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Zhang
authors.forename: Huanyu
authors.initials: H
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
authors.forename: Guodong
authors.initials: G
authors.lastname: Liang
mesh_terms: D000524:Alphavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D018354:Alphavirus Infections / Q000821:virology; D000818:Animals; D002460:Cell Line; D002681:China; D009033:Culicidae / Q000145:classification / Q000502:physiology* / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004672:Encephalitis, Japanese / Q000821:virology; D006801:Humans; D007303:Insect Vectors / Q000145:classification / Q000502:physiology* / Q000821:virology*; D002052:Myanmar; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014780:Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*