title: New Drosophila P-like elements and reclassification of Drosophila P-elements subfamilies.
abstract: Genomic searches for P-like transposable elements were performed (1) in silico in the 12 available Drosophila genomes and (2) by PCR using degenerate primers in 21 Neotropical Drosophila species. In silico searches revealed P-like sequences only in Drosophila persimilis and Drosophila willistoni. Sixteen new P-like elements were obtained by PCR. These sequences were added to sequences of previously described P-like elements, and a phylogenetic analysis was performed. The subfamilies of P-elements described in the literature (Canonical, M, O, T, and K) were included in the reconstructed tree, and all were monophyletic. However, we suggest that some subfamilies can be enlarged, other subdivided, and some new subfamilies may be proposed, totalizing eleven subfamilies, most of which contain new P-like sequences. Our analyses support the monophyly of P-like elements in Drosophilidae. We suggest that, once these elements need host-specific factors to be mobilizable, the horizontal transfer (HT) of P-like elements may be inhibited among more distant taxa. Nevertheless, HT among Drosophilidae species appears to be a common phenomenon.
authors.affiliation: Dep de Biologia, CCNE, Santa Maria Federal University, Av. Roraima 1000, Santa Maria, RS 97105-900, Brazil. elgion.loreto@pq.cnpq.br
authors.forename: Elgion L S
authors.initials: EL
authors.lastname: Loreto
authors.forename: Francis M B
authors.initials: FM
authors.lastname: Zambra
authors.forename: Mauro F
authors.initials: MF
authors.lastname: Ortiz
authors.forename: Lizandra J
authors.initials: LJ
authors.lastname: Robe
mesh_terms: D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D004330:Drosophila / Q000145:classification / Q000235:genetics*; D019143:Evolution, Molecular; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004251:DNA Transposable Elements