title: [Screening and identification of peptides specifically binding to human osteosarcoma cells].
abstract: OBJECTIVE
  To obtain the peptide that specifically binds to human osteosarcoma MG-63 cells from Ph. D. 7TM phage display peptide library.
  
  
  METHODS
  Human osteosarcoma MG-63 cells were used as the target cells with human embryonic kidney 293T cells as the control for screening the peptide from Ph. D. 7TM phage display peptide library. The enriched specially binding peptides were verified by cell enzyme-linked immunosorbent assay (ELISA). The location of the peptide in MG-63 cells was investigated using cell fluorescence staining, and targeting of the peptide was tested by organ immunohistochemistry with Osteosarcoma model.
  
  
  RESULTS
  The specifically binding peptides were enriched after 4 rounds of screening. The sequence SLTNLSK was confirmed as the most frequent peptide by DNA sequencing and showed strong specificity verified by cell ELISA, fluorescent staining and organ immunohistochemistry.
  
  
  CONCLUSIONS
  A peptide that specifically binds to MG-63 cells has been screened from Ph. D. 7TM phage display peptide library to serve as a potential candidate for osteosarcoma-targeting therapy.
authors.affiliation: Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, China. kunlu11@163.com
authors.forename: Kun
authors.initials: K
authors.lastname: Lu
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Guan
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Zhihan
authors.initials: Z
authors.lastname: Li
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Shi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001859:Bone Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012516:Osteosarcoma / Q000188:drug therapy*; D019151:Peptide Library; D010455:Peptides / Q000032:analysis*; D011485:Protein Binding
chemical_list: D019151:Peptide Library; D010455:Peptides