title: Sequence and topological characterization of Toll-like receptor 8 gene of Indian riverine buffalo (Bubalus bubalis).
abstract: In this study, buffalo (Bubalus bubalis) Toll-like receptor 8 (TLR8) gene has been characterized by sequence analysis and detecting polymorphism. Complete ORF of buffalo TLR8 gene was amplified using the RNA isolated from spleen tissue, which was found to be 3,102 nucleotides long encoding a 1,033 amino acid protein. Buffalo TLR8 had 10 nucleotide changes as compared to other livestock species resulting in six unique amino acid changes, four of them lying within leucine-rich repeat (LRR) domains. As compared to cattle (Bos indicus and Bos taurus), out of fifteen cysteine residues, fourteen were conserved and Cys at position 521 was replaced by Arg. Nine of the LRR domains had no amino acid change as compared to cattle, whereas LRR-C-terminus had maximum, five amino acid changes. Sequence characterization of 12 riverine and swamp buffaloes revealed presence of four polymorphic nucleotides, two of them were non-synonymous, one synonymous and one site in 3'UTR. PCR-RFLP genotyping of non-synonymous SNP 2758A>G (ILeu920Val) in Toll-interleukin-1 receptor domain of 463 swamp and riverine buffaloes showed a higher frequency of allele A in swamp (95 %) as compared to riverine (9.84 %) buffaloes.
authors.affiliation: National Bureau of Animal Genetic Resources, GT Road By-Pass, Karnal, 132 001, Haryana, India.
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Dubey
authors.forename: Shubham
authors.initials: S
authors.lastname: Goyal
authors.forename: Jigyasa
authors.initials: J
authors.lastname: Aggarwal
authors.forename: Suresh Kumar
authors.initials: SK
authors.lastname: Gahlawat
authors.forename: Periasamy
authors.initials: P
authors.lastname: Kathiravan
authors.forename: Bishnu Prasad
authors.initials: BP
authors.lastname: Mishra
authors.forename: Ranjit Singh
authors.initials: RS
authors.lastname: Kataria
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D040461:Gene Components; D005787:Gene Frequency; D005838:Genotype; D007194:India; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D051200:Toll-Like Receptor 8 / Q000235:genetics*
chemical_list: D017931:DNA Primers; D051200:Toll-Like Receptor 8