title: Enabling fiber optic serotyping of pathogenic bacteria through improved anti-fouling functional surfaces.
abstract: Significant research efforts are continually being directed towards the development of sensitive and accurate surface plasmon resonance biosensors for sequence specific DNA detection. These sensors hold great potential for applications in healthcare and diagnostics. However, the performance of these sensors in practical usage scenarios is often limited due to interference from the sample matrix. This work shows how the co-immobilization of glycol(PEG) diluents or 'back filling' of the DNA sensing layer can successfully address these problems. A novel SPR based melting assay is used for the analysis of a synthetic oligomer target as well as PCR amplified genomic DNA extracted from Legionella pneumophila. The benefits of sensing layer back filling on the assay performance are first demonstrated through melting analysis of the oligomer target and it is shown how back filling enables accurate discrimination of Legionella pneumophila serogroups directly from the PCR reaction product with complete suppression of sensor fouling.
authors.affiliation: BIOSYST-MeBioS, KU Leuven, Willem De Croylaan 42, Leuven, Belgium.
authors.forename: K P F
authors.initials: KP
authors.lastname: Janssen
authors.forename: K
authors.initials: K
authors.lastname: Knez
authors.forename: L
authors.initials: L
authors.lastname: Vanysacker
authors.forename: J
authors.initials: J
authors.lastname: Schrooten
authors.forename: D
authors.initials: D
authors.lastname: Spasic
authors.forename: J
authors.initials: J
authors.lastname: Lammertyn
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D004866:Equipment Contamination / Q000517:prevention & control*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D012703:Serotyping / Q000295:instrumentation*
chemical_list: D004269:DNA, Bacterial