title: Induction of apoptosis signaling by glycoprotein of Capsosiphon fulvescens in human gastric cancer (AGS) cells.
abstract: Capsosiphon fulvescens is a well-known green sea algae that has been touted in recent years as a potential anticancer drug. In this study, C. fulvescens glycoprotein (Cf-GP) showed proapoptotic signaling in AGS cells. An MTS assay indicated that Cf-GP inhibited the proliferation of AGS cell lines in a dose-dependent manner. Cells were treated with Cf-GP and the expression of proteins associated with apoptosis was examined by Western blotting. Based on the Western blot, expression of Cf-GP-activated caspase-cascade and PARP, which is a substrate of caspase-3 and -8, and proteins of the Bcl-2 family was observed. Cf-GP treatment stimulated the release of cytochrome C and apoptotic protease activating factor-1 from mitochondria to the cytosol. Cf-GP inhibited the growth of AGS cells through induction of sub-G1 phase arrest. We confirmed that sub-G1-phase arrest was associated with a decrease in the expression of cyclin D, cyclin E, Cdk2, Cdk4, and Cdk6, and an increase in the protein levels of p21 and p27. As a result, the increased sub-G1 ratio appears to be inhibited by cell proliferation. Therefore, we can confirm apoptosis in the AGS cells. Our results suggest that Cf-GP could be a potential source of biofunctional material that shows anticancer effects in human gastrointestinal cancer.
authors.affiliation: Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea.
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Kim
authors.forename: In-Hye
authors.initials: IH
authors.lastname: Kim
authors.forename: Taek-Jeong
authors.initials: TJ
authors.lastname: Nam
mesh_terms: D020418:Algal Proteins / Q000009:adverse effects / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000009:adverse effects / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D000460:Chlorophyta / Q000737:chemistry*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D006023:Glycoproteins / Q000009:adverse effects / Q000494:pharmacology*; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects; D055670:Medicine, Korean Traditional; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D010940:Plant Proteins / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D020418:Algal Proteins; D000972:Antineoplastic Agents, Phytogenic; D051017:Apoptosis Regulatory Proteins; D006023:Glycoproteins; D009363:Neoplasm Proteins; D010940:Plant Proteins; D011065:Poly(ADP-ribose) Polymerases