title: MYOD-1 in normal colonic mucosa--role as a putative biomarker?
abstract: BACKGROUND
  DNA methylation of promoter-associated CpG islands of certain genes may play a role in the development of colorectal cancer. The MYOD-1 gene which is a muscle differentiation gene has been showed to be significantly methylated in colorectal cancer which, is an age related event. However the role of this gene in the colonic mucosa is not understood and whether methylation occurs in subjects without colon cancer. In this study, we have determined the frequency of methylation of the MYOD-1 gene in normal colonic mucosa and investigated to see if this is associated with established colorectal cancer risk factors primarily ageing.
  
  
  RESULTS
  We analysed colonic mucosal biopsies in 218 normal individuals and demonstrated that in most individuals promoter hypermethylation was not quantified for MYOD-1. However, promoter hypermethylation increased significantly with age (p<0.001 using regression analysis) and this was gender independent. We also showed that gene promoter methylation increased positively with an increase in waist to hip (WHR) ratio - the latter is also a known risk factor for colon cancer development.
  
  
  CONCLUSIONS
  Our study suggests that promoter gene hypermethylation of the MYOD-1 gene increases significantly with age in normal individuals and thus may offer potential as a putative biomarker for colorectal cancer.
authors.affiliation: Department of Gastroenterology, University Hospital Coventry & Warwickshire, Coventry, UK. r.arasaradnam@warwick.ac.uk
authors.forename: Ramesh P
authors.initials: RP
authors.lastname: Arasaradnam
authors.forename: M Nabil
authors.initials: MN
authors.lastname: Quraishi
authors.forename: Daniel
authors.initials: D
authors.lastname: Commane
authors.forename: John C
authors.initials: JC
authors.lastname: Mathers
authors.forename: Mike
authors.initials: M
authors.lastname: Bradburn
mesh_terms: D000328:Adult; D000375:Aging / Q000235:genetics; D000886:Anthropometry; D015415:Biomarkers / Q000378:metabolism; D003106:Colon / Q000378:metabolism*; D019175:DNA Methylation / Q000235:genetics; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008019:Life Style; D008297:Male; D008875:Middle Aged; D017570:MyoD Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein