title: Electrophoretic separation and detection of metalloproteins by X-ray fluorescence mapping.
abstract: All living systems depend on metalloproteins. Yet, while tools for the separation and identification of apo-proteins are well developed, those enabling identification and quantitation of individual metalloproteins within complex mixtures are still nascent. Here, we describe the electrophoretic separation of a mixture of carbonic anhydrase, ceruloplasmin, urease, and hemoglobin using native 2D gel electrophoresis and X-ray fluorescence mapping-an approach we have developed to be broadly applicable, not require specialized equipment for sample preparation, and likely to be extensible in the future.
authors.affiliation: Biosciences Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL, USA.
authors.forename: Tripti
authors.initials: T
authors.lastname: Khare
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Chishti
authors.forename: Lydia A
authors.initials: LA
authors.lastname: Finney
mesh_terms: D000818:Animals; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000302:isolation & purification*; D002417:Cattle; D002570:Ceruloplasmin / Q000737:chemistry / Q000302:isolation & purification*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008667:Metalloproteins / Q000737:chemistry / Q000302:isolation & purification; D008670:Metals / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D011487:Protein Conformation; D013052:Spectrometry, X-Ray Emission / Q000379:methods*; D017356:Synchrotrons; D014510:Urease / Q000737:chemistry / Q000302:isolation & purification*; D056928:X-Ray Absorption Spectroscopy / Q000379:methods
chemical_list: D006454:Hemoglobins; D008667:Metalloproteins; D008670:Metals; D010940:Plant Proteins; D002570:Ceruloplasmin; D014510:Urease; D002256:Carbonic Anhydrases