title: The protective effect of docosahexaenoic acid on the bilirubin neurotoxicity.
abstract: Usually, all newborns demonstrate high serum unconjugated bilirubin (UCB) level. UCB may induce adverse effects in the central nervous system. We aimed to evaluate the cytotoxic effects of UCB and the protective effects of docosahexaenoic acid (DHA) on astrocyte cell cultures. The viability of astrocyte cells decreased after UCB treatment in a dose-dependent manner. Pre-incubation of DHA prevents the cells from UCB-mediated neurotoxicity. Our results shown that UCB leads to inhibition of antioxidant enzymes superoxide dismutase (SOD), catalase and GPx activity and induction of apoptosis. But only 4-h pretreatment of DHA can suppress of UCB-mediated inhibition of antioxidant enzymes SOD, catalase and GPx activity and induction of apoptosis in astrocytes. Our results strongly indicated that DHA has a protective effect on UCB-mediated neurotoxicity through inhibition apoptosis and antioxidant enzymes activity of SOD, CAT and GPx in rat primer astrocyte cell line.
authors.affiliation: Department of Pediatrics, Pamukkale University Faculty of Medicine, Denizli, Turkey.
authors.forename: Cem
authors.initials: C
authors.lastname: Becerir
authors.forename: İlknur
authors.initials: İ
authors.lastname: Kılıç
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Sahin
authors.forename: Özmert
authors.initials: Ö
authors.lastname: Özdemir
authors.forename: Onur
authors.initials: O
authors.lastname: Tokgün
authors.forename: Bülent
authors.initials: B
authors.lastname: Özdemir
authors.forename: Hakan
authors.initials: H
authors.lastname: Akca
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects*; D001663:Bilirubin / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004281:Docosahexaenoic Acids; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001663:Bilirubin