title: Combined chemotherapy and ALA-based photodynamic therapy in leukemic murine cells.
abstract: The effects of combined administration of doxorubicin (DOX) and vincristine (VCR), with 5-aminolevulinic acid photodynamic treatment (ALA-PDT), were analyzed in sensitive murine leukemic cell lines (LBR-) and DOX and VCR chemoresistant LBR-D160 and LBR-V160 cell lines. Low doses of DOX and VCR increased anti-cancer effect of ALA-PDT in LBR-cells. Decrease in cell survival was higher when the combination VCR+ALA-PDT was used compared to DOX+ALA-PDT. Resistant cell lines LBR-D160 and LBR-V160 were sensitive to ALA-PDT; however, no changes occured when combining therapies. Thus, ALA-PDT can overcome drug resistance and is a good candidate for using treating multidrug resistant (MDR) cells.
authors.affiliation: Porphyirins and Porphyrias Research Center (CIPYP), National Research Council (CONICET), Hospital of Clinics, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Berenice
authors.initials: B
authors.lastname: Diez
authors.forename: Glenda
authors.initials: G
authors.lastname: Ernst
authors.forename: María Julieta
authors.initials: MJ
authors.lastname: Teijo
authors.forename: Alcira
authors.initials: A
authors.lastname: Batlle
authors.forename: Silvia
authors.initials: S
authors.lastname: Hajos
authors.forename: Haydée
authors.initials: H
authors.lastname: Fukuda
mesh_terms: D000622:Aminolevulinic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D004317:Doxorubicin / Q000008:administration & dosage; D019008:Drug Resistance, Neoplasm / Q000187:drug effects / Q000528:radiation effects; D007938:Leukemia / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D009336:Necrosis / Q000139:chemically induced / Q000209:etiology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D014750:Vincristine / Q000008:administration & dosage
chemical_list: D017319:Photosensitizing Agents; D014750:Vincristine; D004317:Doxorubicin; D000622:Aminolevulinic Acid