title: SRAP analysis of genetic diversity of nine  native populations of wild sugarcane, Saccharum spontaneum, from Sichuan, China.
abstract: Saccharum spontaneum is a wild sugarcane species that is native to and  widely distributed in China. It has been extensively used in sugarcane breeding  programs, and is being tested for the development of bioenergy cultivars. In  order to provide basic information for the exploitation of this species, we  analyzed genetic variation among and within native S. spontaneum populations  collected from Sichuan, China. Eighty plants from nine native populations were  sampled. Twenty-one sequence-related amplified polymorphism primer pairs  generated 235 clearly scorable bands, of which 185 were polymorphic (78.7%).  Nei's genetic diversity was 0.2801 and Shannon's information index was 0.4155  across the populations. Genetic diversity parameters, G(ST) value (0.2088) and N(m) value (1.8944), showed that the genetic variation  within populations was greater than that among populations. In the cluster  analysis, one major grouping was formed by populations from Ya'an and another  one by populations from Sichuan basin; a population from Baoxing formed a  single cluster. In order to fully comprehend the genetic diversity of  cold-tolerant local germplasm in this species, germplasm should be collected  from the heterogeneous environments along the northern regions of this species'  distribution. The germplasm that we collected should be a valuable resource for Saccharum breeding.
authors.affiliation: Sichuan Academy of Grassland Sciences, Chengdu, Sichuan, China.
authors.forename: D
authors.initials: D
authors.lastname: Chang
authors.forename: F Y
authors.initials: FY
authors.lastname: Yang
authors.forename: J J
authors.initials: JJ
authors.lastname: Yan
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Wu
authors.forename: S Q
authors.initials: SQ
authors.lastname: Bai
authors.forename: X Z
authors.initials: XZ
authors.lastname: Liang
authors.forename: Y W
authors.initials: YW
authors.lastname: Zhang
authors.forename: Y M
authors.initials: YM
authors.lastname: Gan
mesh_terms: D001483:Base Sequence; D002681:China; D017931:DNA Primers; D014644:Genetic Variation; D016133:Polymerase Chain Reaction; D031786:Saccharum / Q000235:genetics*
chemical_list: D017931:DNA Primers