title: Liposomal glyco-microarray for studying glycolipid-protein interactions.
abstract: A microarray enables high-throughput interaction screening of numerous biomolecules; however, fabrication of a microarray composed of cellular membrane components has proven difficult. We report fabrication of a liposomal glyco-microarray by using an azide-reactive liposome that carries synthetic and natural glycolipids via chemically selective and biocompatible liposome immobilization chemistry. Briefly, liposomes carrying anchor lipid dipalmitoylphosphatidylethanolamine (DPPE)-PEG(2000)-triphenylphosphine and ganglioside (GM1 or GM3) were prepared first and were then printed onto an azide-modified glass slide so as to afford a liposomal glyco-microarray via Staudinger ligation. Fluorescent dye release kinetics and fluorescence imaging confirmed successful liposome immobilization and specific protein binding to the intact arrayed glycoliposomes. The liposomal glyco-microarray with different gangliosides showed their specific lectin and toxin binding with different binding affinity. The azide-reactive liposome provides a facile strategy for fabrication of either a natural or a synthetic glycolipid-based membrane-mimetic glycoarray. This liposomal glyco-microarray is simple and broadly applicable and thus will find important biomedical applications, such as studying glycolipid-protein interactions and toxin screening applications.
authors.affiliation: Department of Chemistry, Cleveland State University, Cleveland, OH 44115, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ma
authors.forename: Irena
authors.initials: I
authors.lastname: Sobkiv
authors.forename: Valentinas
authors.initials: V
authors.lastname: Gruzdys
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhang
authors.forename: Xue-Long
authors.initials: XL
authors.lastname: Sun
mesh_terms: D002772:Cholera Toxin / Q000737:chemistry / Q000378:metabolism*; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D006017:Glycolipids; D008081:Liposomes; D037121:Plant Lectins; D002772:Cholera Toxin