title: A facile inhibitor screening of SARS coronavirus N protein using nanoparticle-based RNA oligonucleotide.
abstract: Hundreds of million people worldwide have been infected with severe acute respiratory syndrome (SARS), and the rate of global death from SARS has remarkably increased. Hence, the development of efficient drug treatments for the biological effects of SARS is highly needed. We have previously shown that quantum dots (QDs)-conjugated RNA oligonucleotide is sensitive to the specific recognition of the SARS-associated coronavirus (SARS-CoV) nucleocapsid (N) protein. In this study, we found that a designed biochip could analyze inhibitors of the SARS-CoV N protein using nanoparticle-based RNA oligonucleotide. Among the polyphenolic compounds examined, (-)-catechin gallate and (-)-gallocatechin gallate demonstrated a remarkable inhibition activity on SARS-CoV N protein. (-)-catechin gallate and (-)-gallocatechin gallate attenuated the binding affinity in a concentrated manner as evidenced by QDs-conjugated RNA oligonucleotide on a designed biochip. At a concentration of 0.05 μg mL(-1), (-)-catechin gallate and (-)-gallocatechin gallate showed more than 40% inhibition activity on a nanoparticle-based RNA oligonucleotide biochip system.
authors.affiliation: Division of Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup, Republic of Korea. chroh@kaeri.re.kr
authors.forename: Changhyun
authors.initials: C
authors.lastname: Roh
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D001483:Base Sequence; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D000086462:Coronavirus Nucleocapsid Proteins; D008826:Microbial Sensitivity Tests / Q000379:methods*; D050997:Nanomedicine; D053758:Nanoparticles; D009690:Nucleic Acid Conformation; D019590:Nucleocapsid Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D009843:Oligoribonucleotides / Q000737:chemistry / Q000235:genetics; D040081:Protein Array Analysis; D045663:Quantum Dots; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D045473:Severe acute respiratory syndrome-related coronavirus / Q000187:drug effects* / Q000235:genetics
chemical_list: D000998:Antiviral Agents; D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D009843:Oligoribonucleotides; D012367:RNA, Viral; C417940:gallocatechin gallate; C417939:catechin gallate; D002392:Catechin