title: Silver staining techniques of polyacrylamide gels.
abstract: Although the main application for polyacrylamide gels is the separation and subsequent blotting of proteins for immunodetection, there are tasks that need staining of proteins in the polyacrylamide gel. Several different staining techniques exist for protein staining in SDS gels that differ in their sensitivity, their expenditure of time, and other aspects. Still, silver staining is the most sensitive and reliable staining technique. Because this technique was developed in the 1970s, a huge number of variations exist. Therefore, we will provide herein three methods, which are robust and easy to perform.
authors.affiliation: Carl Gustav Carus University TU Dresden, Dresden, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Bartsch
authors.forename: Claudia
authors.initials: C
authors.lastname: Arndt
authors.forename: Stefanie
authors.initials: S
authors.lastname: Koristka
authors.forename: Marc
authors.initials: M
authors.lastname: Cartellieri
authors.forename: Michael
authors.initials: M
authors.lastname: Bachmann
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D057230:Limit of Detection; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification; D016622:Silver Staining / Q000379:methods*
chemical_list: D000180:Acrylic Resins; C016680:polyacrylamide gels; D012710:Serum Albumin, Bovine