title: Expression of bacterial GshF in Pichia pastoris for glutathione production.
abstract: Conventionally, two consecutive enzymatic reactions catalyzed by γ-glutamylcysteine synthetase and glutathione synthetase are most commonly used for glutathione production. Here we demonstrate that bacterial bifunctional GshF can be used for glutathione production in a eukaryotic system without accumulation of the intermediate γ-glutamylcysteine.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Shaolin
authors.initials: S
authors.lastname: Ge
authors.forename: Taicheng
authors.initials: T
authors.lastname: Zhu
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
mesh_terms: D001709:Biotechnology / Q000379:methods*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004151:Dipeptides / Q000378:metabolism; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism*; D005978:Glutathione / Q000096:biosynthesis*; D005981:Glutathione Synthase / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000235:genetics; D016979:Pasteurella multocida / Q000235:genetics; D010843:Pichia / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D013292:Streptococcus agalactiae / Q000235:genetics
chemical_list: D017931:DNA Primers; D004151:Dipeptides; D011994:Recombinant Proteins; D005721:Glutamate-Cysteine Ligase; D005981:Glutathione Synthase; D005978:Glutathione; C017341:gamma-glutamylcysteine