title: Direct infusion electrospray ionization-ion mobility high resolution mass spectrometry (DIESI-IM-HRMS) for rapid characterization of potential bioprocess streams.
abstract: Direct infusion electrospray ionization - ion mobility - high resolution mass spectrometry (DIESI-IM-HRMS) has been utilized as a rapid technique for the characterization of total molecular composition in "whole-sample" biomass hydrolysates and extracts. IM-HRMS data reveal a broad molecular weight distribution of sample components (up to 1100 m/z) and provide trendline isolation of feedstock components from those introduced "in process." Chemical formulas were obtained from HRMS exact mass measurements (with typical mass error less than 5 ppm) and were consistent with structural carbohydrates and other lignocellulosic degradation products. Analyte assignments are supported via IM-MS collision-cross-section measurements and trendline analysis (e.g., all carbohydrate oligomers identified in a corn stover hydrolysate were found to fall within 6% of an average trendline). These data represent the first report of collision cross sections for several negatively charged carbohydrates and other acidic species occurring natively in biomass hydrolysates.
authors.affiliation: Department of Chemistry and Biochemistry, Baylor University, Waco, TX 76798, USA.
authors.forename: Sharon M
authors.initials: SM
authors.lastname: Munisamy
authors.forename: C Kevin
authors.initials: CK
authors.lastname: Chambliss
authors.forename: Christopher
authors.initials: C
authors.lastname: Becker
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000032:analysis / Q000737:chemistry; D045868:Sorghum / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013464:Sulfuric Acids / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D013464:Sulfuric Acids; C036909:lignocellulose; D008031:Lignin; C033158:sulfuric acid