title: Differential diagnostic value of GPC3-CD34 combined staining in small liver nodules with diameter less than 3 cm.
abstract: The diagnostic value of combining glypican-3 (GPC3) and CD34 staining for small nodules in liver biopsy specimens has not been evaluated. In this study, 201 thin-core biopsy specimens were assessed using GPC3 and CD34 immunochemical staining, including 33 cirrhotic regenerative nodules, 31 high-grade dysplastic nodules, 70 hepatocellular carcinomas (HCCs) with nodules 3 cm or smaller, and 67 HCCs with nodules larger than 3 cm. The results showed that the accuracy of GPC3 staining (90.3%) among liver nodules 3 cm or smaller was better than its use among all nodules (P = .045). Furthermore, the positive expression rate of costaining was significantly greater than that observed for GPC3 or CD34 single staining (P < .001 and P = .002, respectively). These data demonstrate that GPC3 staining is more accurate for the diagnosis of HCC on thin-core biopsy specimens in nodules 3 cm or smaller compared with its use in all nodules, while GPC3 and CD34 costaining has better diagnostic value than does single staining.
authors.affiliation: Department of Gastroenterology and Hepatology, The Third Central Hospital of Tianjin, Tianjin 300170, China.
authors.forename: Fengmei
authors.initials: F
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Jing
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Ge
authors.initials: G
authors.lastname: Li
authors.forename: Tinghong
authors.initials: T
authors.lastname: Li
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Yijun
authors.initials: Y
authors.lastname: Wang
authors.forename: Yingtang
authors.initials: Y
authors.lastname: Gao
authors.forename: Tao
authors.initials: T
authors.lastname: Han
authors.forename: Zhi
authors.initials: Z
authors.lastname: Du
mesh_terms: D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D001707:Biopsy, Needle; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000473:pathology; D003937:Diagnosis, Differential; D053673:Glypicans / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000473:pathology*; D008103:Liver Cirrhosis / Q000175:diagnosis* / Q000473:pathology; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000473:pathology; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D053673:Glypicans