title: Aqueous two-phase affinity partitioning systems: current applications and trends.
abstract: Aqueous two-phase systems (ATPS) have been studied and used for product recovery and purification from diverse biological sources. ATPS are characterized by their versatility, easy scale up parameters, process integration capability and relative low cost. This technique is commonly regarded as a primary recovery stage mainly due to its low selectivity. However, the use of strategies involving the modification of ATPS with affinity ligands have resulted in significant increases in recovery yields and purification folds of biological products. The aim of this review is to highlight current applications, trends and challenges regarding affinity partitioning in aqueous two-phase systems for the fractionation, recovery and purification of biological products.
authors.affiliation: Centro de Biotecnología FEMSA, Departamento de Biotecnología e Ingeniería de Alimentos, Tecnológico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL 64849, Mexico.
authors.forename: Federico
authors.initials: F
authors.lastname: Ruiz-Ruiz
authors.forename: Jorge
authors.initials: J
authors.lastname: Benavides
authors.forename: Oscar
authors.initials: O
authors.lastname: Aguilar
authors.forename: Marco
authors.initials: M
authors.lastname: Rito-Palomares
mesh_terms: D000818:Animals; D006801:Humans; D059625:Liquid-Liquid Extraction / Q000379:methods*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification; D014867:Water / Q000737:chemistry*
chemical_list: D011108:Polymers; D011506:Proteins; D014867:Water