title: New virus discovery by deep sequencing of small RNAs.
abstract: Small RNAs (sRNAs) have emerged as one of the most important regulators of gene expression in eukaryotes. sRNAs are intermediate molecules as well as end products in the antiviral defense pathway called RNA interference in plants and animals. Profiling of sRNAs using next-generation sequencing technologies has identified a number of plant viruses that have never been reported previously, and has provided a deeper view of virus populations in a plant that cannot be achieved by conventional methods like PCR and ELISA. In this chapter, we describe the methodology of deep sequencing of sRNAs. The high-throughput and highly sensitive method will revolutionize the identification of plant viruses and the study of molecular plant-virus interactions.
authors.affiliation: Center for Grapevine Biotechnology, William H. Darr School of Agriculture, Missouri State University, Mountain Grove, MO, USA. kashmir123@gmail.com
authors.forename: Kashmir
authors.initials: K
authors.lastname: Singh
authors.forename: Ravneet
authors.initials: R
authors.lastname: Kaur
authors.forename: Wenping
authors.initials: W
authors.lastname: Qiu
mesh_terms: D002021:Buffers; D020451:Contig Mapping; D018076:DNA, Complementary / Q000138:chemical synthesis / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D058889:Molecular Typing; D010942:Plant Viruses / Q000235:genetics*; D016133:Polymerase Chain Reaction; D058727:RNA, Small Untranslated / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D017423:Sequence Analysis, RNA; D012984:Software; D027843:Vitis / Q000821:virology
chemical_list: D002021:Buffers; D018076:DNA, Complementary; D058727:RNA, Small Untranslated; D012367:RNA, Viral