title: Vascular effects and electrolyte homeostasis of the natriuretic peptide isolated from Crotalus oreganus abyssus (North American Grand Canyon rattlesnake) venom.
abstract: Crotalus oreganus abyssus is a rattlesnake that is usually found in the Grand Canyon, United States of America. Knowledge regarding the composition of C. o. abyssus venom is scarce. New natriuretic peptides (NPs) have been isolated and characterized from the venoms of members of the Crotalinae family. The NP family comprises three members, ANP (atrial natriuretic peptide), BNP (b-type natriuretic peptide) and CNP (c-type natriuretic peptide), and has an important role in blood pressure regulation and electrolyte homeostasis. The aim of the present study was to characterize a novel natriuretic-like peptide (Coa_NP2), isolated from C. o. abyssus venom. The Coa_NP2 presents an average molecular mass of 3419.88Da (theoretical average molecular mass 3418.94Da, monoisotopic molecular mass 3416.66Da and theoretical PI 7.78) and its amino acid sequence presents the loop region that is characteristic of natriuretic peptides. The peptide has 32 amino acids and its complete sequence is SYGISSGCFGLKLDRIGTMSGLGCWRLLQDSP. Coa_NP2 is a natriuretic peptide of the ANP/BNP-like family, since the carboxyterminal region of CNP has its own NP domain. We demonstrate, herein, that Coa_NP2 produces a dose-dependent decrease in mean arterial pressure in rats, followed by significant increases in concentrations of markers of nitric oxide formation measured in the plasma and vasorelaxation in a thoracic aortic ring bath. The structural and biological aspects confirm Coa_NP2 as a new natriuretic peptide, isolated from snake venom.
authors.affiliation: Federal University of São João Del Rei Campus Alto Paraopeba, Ouro Branco, Minas Gerais, Brazil.
authors.forename: S L
authors.initials: SL
authors.lastname: Da Silva
authors.forename: C A
authors.initials: CA
authors.lastname: Dias-Junior
authors.forename: P A
authors.initials: PA
authors.lastname: Baldasso
authors.forename: D C S
authors.initials: DC
authors.lastname: Damico
authors.forename: B M A
authors.initials: BM
authors.lastname: Carvalho
authors.forename: A
authors.initials: A
authors.lastname: Garanto
authors.forename: G
authors.initials: G
authors.lastname: Acosta
authors.forename: E
authors.initials: E
authors.lastname: Oliveira
authors.forename: F
authors.initials: F
authors.lastname: Albericio
authors.forename: A M
authors.initials: AM
authors.lastname: Soares
authors.forename: S
authors.initials: S
authors.lastname: Marangoni
authors.forename: R R
authors.initials: RR
authors.lastname: Resende
mesh_terms: D000818:Animals; D062186:Arterial Pressure / Q000187:drug effects; D017839:Crotalus; D004573:Electrolytes / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects; D008297:Male; D045265:Natriuretic Peptides / Q000737:chemistry* / Q000494:pharmacology*; D009569:Nitric Oxide / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012910:Snake Venoms / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004573:Electrolytes; D045265:Natriuretic Peptides; D012910:Snake Venoms; D009569:Nitric Oxide