title: Interspecies differences in membrane-associated protease activities of thyrocytes and their relevance for thyroid cancer studies.
abstract: BACKGROUND
  To understand the role of proteases involved in human thyroid cancer progression and tissue invasion, thyrocytes from other species could potentially be used provided their characteristics are similar. It is not known whether dipeptidyl peptidase IV and aminopeptidase N activities, which are overexpressed in human thyroid cancer, are, as in human, also absent in normal thyrocytes of other species, making them suitable models for studies on the regulation of these proteases.
  
  
  METHODS
  To assess the role of these proteases, activity was measured in thyroid tissue of human, mouse, rat, porcine, bovine and ovine origin. The lysosomal protease, dipeptidyl peptidase II, was used for comparison.
  
  
  RESULTS
  Murine, rat, ovine, bovine and human thyrocytes all lacked dipeptidyl peptidase IV and aminopeptidase N activity, but porcine thyrocytes were found to possess both. In contrast, lysosomal dipeptidyl peptidase II was strongly expressed in all species. These activity patterns were maintained in cultured cells. Cultured porcine thyrocytes formed follicles with typical morphology upon stimulation with TSH but differed from human thyrocytes in their response to thiamazole.
  
  
  CONCLUSIONS
  These species differences in the expression of dipeptidyl peptidase IV and aminopeptidase N, indicate that porcine thyrocytes cannot be considered appropriate for the study of proteases in human cancer development.
authors.affiliation: Department of Endocrinology, University of Tuebingen, Tuebingen, Germany.
authors.forename: Eleonore
authors.initials: E
authors.lastname: Fröhlich
authors.forename: Elke
authors.initials: E
authors.lastname: Maier
authors.forename: Richard
authors.initials: R
authors.lastname: Wahl
mesh_terms: D000818:Animals; D018826:CD13 Antigens / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017207:Rats, Sprague-Dawley; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology* / Q000201:enzymology*; D013964:Thyroid Neoplasms / Q000201:enzymology* / Q000473:pathology*
chemical_list: D018826:CD13 Antigens; D018819:Dipeptidyl Peptidase 4