title: Efficient expansion of mesenchymal stem cells from mouse bone marrow under hypoxic conditions.
abstract: To realize the therapeutic potential of mesenchymal stem cells (MSCs), a large number of high-quality MSCs isolated from different species, such as mouse, were acquired for preclinical animal studies. Surprisingly, isolation and purification of mouse MSCs (mMSCs) is arduous because of the low frequency of MSCs and contamination of haematopoietic cells in culture. We have developed a method based on low density and hypoxic culture to isolate and expand mMSCs from different strains, including BALB/c, C57BL/6J, FVB/N and DBA/2. The cells from all of the strains expanded more rapidly when plated at low density in hypoxic culture compared with normoxic culture. These cells expressed CD44, CD105, CD29 and Sca-1 markers but not CD11b, CD34, CD45 and CD31 markers. Moreover, they were able to differentiate along osteoblastic, adipocytic and chondrocytic lineages. In conclusion, we have developed a robust method for isolation and expansion of mMSCs by combining low-density culture with hypoxic culture.
authors.affiliation: Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; Institute of Anatomy and Cell Biology, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Tu-Lai
authors.initials: TL
authors.lastname: Yew
authors.forename: Ming-Chau
authors.initials: MC
authors.lastname: Chang
authors.forename: Yuan-Tong
authors.initials: YT
authors.lastname: Hsu
authors.forename: Fan-Yu
authors.initials: FY
authors.lastname: He
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Weng
authors.forename: Chih-Chien
authors.initials: CC
authors.lastname: Tsai
authors.forename: Fang-Yao
authors.initials: FY
authors.lastname: Chiu
authors.forename: Shih-Chieh
authors.initials: SC
authors.lastname: Hung
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D015687:Cell Hypoxia; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice