title: Conjugated linoleic acid supplementation caused reduction of perilipin1 and aberrant lipolysis in epididymal adipose tissue.
abstract: Perilipin1, a coat protein of lipid droplet, plays a key role in adipocyte lipolysis and fat formation of adipose tissues. However, it is not clear how the expression of perilipin1 is affected in the decreased white adipose tissues (WAT) of mice treated with dietary supplement of conjugated linoleic acids (CLA). Here we obtained lipodystrophic mice by dietary administration of CLA which exhibited reduced epididymal (EPI) WAT, aberrant adipocytes and decreased expression of leptin in this tissue. We found both transcription and translation of perilipin1 was suppressed significantly in EPI WAT of CLA-treated mice compared to that of control mice. The gene expression of negative regulator tumor necrosis factor α (TNFα) and the positive regulator Peroxisome Proliferator-Activated Receptor-γ (PPARγ) of perilipin1 was up-regulated and down-regulated, respectively. In cultured 3T3-L1 cells the promoter activity of perilipin1 was dramatically inhibited in the presence of CLA. Using ex vivo experiment we found that the basal lipolysis was elevated but the hormone-stimulated lipolysis blunted in adipose explants of CLA-treated mice compared to that of control mice, suggesting that the reduction of perilipin1 in white adipose tissues may at least in part contribute to CLA-mediated alternation of lipolysis of WAT.
authors.affiliation: College of Animal Sciences and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan Province, People's Republic of China.
authors.forename: Demin
authors.initials: D
authors.lastname: Cai
authors.forename: Hongji
authors.initials: H
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Liqiang
authors.initials: L
authors.lastname: Han
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
authors.forename: Guoyu
authors.initials: G
authors.lastname: Yang
authors.forename: Guoqing
authors.initials: G
authors.lastname: Yang
mesh_terms: D041721:3T3-L1 Cells; D052436:Adipose Tissue, White / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D019587:Dietary Supplements; D004435:Eating; D004822:Epididymis / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage*; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D051379:Mice; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D011401:Promoter Regions, Genetic
chemical_list: D002352:Carrier Proteins; D044243:Linoleic Acids, Conjugated; D000070778:Perilipin-1; D010750:Phosphoproteins