title: Identification of differentially expressed genes in a spontaneous altered leaf shape mutant of the navel orange [Citrus sinensis (L.) Osbeck].
abstract: Most of the economically important citrus cultivars have originated from bud mutations. Leaf shape and structure are important factors that impact plant photosynthesis. We found a spontaneous bud mutant exhibiting a narrow leaf phenotype in navel orange [Citrus sinensis (L.) Osbeck]. To identify and characterize the genes involved in the formation of this trait, we performed suppression subtractive hybridization (SSH) and macroarray analysis. A total of 221 non-redundant differentially expressed transcripts were obtained. These transcripts included cell wall- and microtubule-related genes and two transcription factor-encoding genes, yabby and wox, which are crucial for leaf morphogenesis. Many highly redundant transcripts were associated with stress responses, while others, encoding caffeic acid 3-O-methyltransferase (EC 2.1.1.68) and a myb-like transcription factor, might be involved in the lignin pathway, which produces a component of secondary walls. Furthermore, real-time quantitative RT-PCR was performed for selected genes to validate the quality of the expressed sequence tags (ESTs) from the SSH libraries. This study represents an attempt to investigate the molecular mechanism associated with a leaf shape mutation, and its results provide new clues for understanding leaf shape mutations in citrus.
authors.affiliation: Key Laboratory of Horticultural Plant Biology-Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, No. 1 Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China. daxinlei@webmail.hzau.edu.cn
authors.forename: Xinlei
authors.initials: X
authors.lastname: Da
authors.forename: Keqin
authors.initials: K
authors.lastname: Yu
authors.forename: Shihui
authors.initials: S
authors.lastname: Shen
authors.forename: Yajian
authors.initials: Y
authors.lastname: Zhang
authors.forename: Juxun
authors.initials: J
authors.lastname: Wu
authors.forename: Hualin
authors.initials: H
authors.lastname: Yi
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D002473:Cell Wall; D002957:Citrus / Q000235:genetics* / Q000378:metabolism; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling; D015723:Gene Library; D017343:Genes, Plant; D008031:Lignin / Q000235:genetics / Q000378:metabolism; D046228:Microarray Analysis; D008870:Microtubules; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D018515:Plant Leaves / Q000033:anatomy & histology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D008031:Lignin