title: [Influence of nutrient sources on Anabaena spiroides growth and odorous compounds production characteristics].
abstract: The occurrence of taste and odors, produced by secondary metabolites of cyanobacteria, has been one of the major water quality problems in drinking water. However, the odorous compounds produced by cyanobacteria usually differ significantly with different species. One cyanobacterium isolated from Yanghe reservoir was identified as Anabaena sp., which can produce high level of geosmin consistently during laboratory culture. By culture expanding experiments, the algal growth and geosmin production characteristics of the Anabaena sp. were studied on different conditions of nitrogen and phosphorus sources. The results indicated that geosmin mainly remained in the intracellular algal cells regardless of the nutrient sources, and the extracellular content was only in th range of 0.2% - 9.6%. Compared with ammonia nitrogen conditions, the growth of Anabaena sp. in nitrate nitrogen conditions was much higher, with a 1.4-fold variation in geosmin production. While ammonia nitrogen concentration was 0.5 mg/L, the algal biomass and geosmin production achieved the highest level of 3.8 x 10(4) cells, mL(-1) and 1.1 x 10(4) ngL(-1), respectively. When the nitrate nitrogen concentration was 2.0 mg/L, the algal biomass and geosmin production achieved the highest level of 6.6 x 10(4) cells x mL(-1) and 1.3 x 10(4) ng x L(-1), respectively. Compared with nitrogen sources, the growth of Anabaena sp. could be promoted significantly until phosphorus level attained 0.12 mg/L, indicating that phosphorus is the main limiting nutrient source for Anabaena sp.. For Yanghe reservoir, the nutrient level has already been enough for the growth of Anabaena sp. Therefore, the nutrient source content, especially phosphorus, should be reduced effectively to control the cyanobacterium bloom and taste and odor problems.
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. jwyu@rcees.ac.cn
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Yu
authors.forename: Ke-Yun
authors.initials: KY
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Su
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Dai-Cheng
authors.initials: DC
authors.lastname: Liu
mesh_terms: D017033:Anabaena / Q000254:growth & development* / Q000378:metabolism; D002681:China; D009284:Naphthols / Q000378:metabolism*; D009584:Nitrogen / Q000032:analysis; D009812:Odorants; D010758:Phosphorus / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014876:Water Pollution / Q000032:analysis*; D014881:Water Supply / Q000032:analysis*
chemical_list: D009284:Naphthols; D014874:Water Pollutants, Chemical; D010758:Phosphorus; C001278:geosmin; D009584:Nitrogen