title: [Effects of citreoviridin on the expression of MCP-1 and ILs induced by TNF-alpha in vein endothelial cells].
abstract: OBJECTIVE
  To investigate the effects of citreoviridin (CIT) on the expression of MCP-1, IL-1beta, IL-6 and IL-8 induced by TNF-alpha in human umbilical vein endothelial cells (HUVECs).
  
  
  METHODS
  HUVECs isolated from the umbilical cord of neonates within 1 hour after birth (informed with consent form) were cultured in DMEM/ F12 media. After 80% of HUVECs were confluent, the cells were divided into four groups and treated with CIT (2 micromol/L) and/or TNF-alpha (10 microg/L). The levels of MCP-1, IL-1beta, IL-6 and IL-8 in the supernatant of cell culture media were measured by ELISA, the activation of NF-kappaB in HUVECs was detected by immunofluorescence staining, and the expression of MCP-1 mRNA was determinated by RT-PCR assay.
  
  
  RESULTS
  The levels of MCP-1, IL-1beta, IL-6 and IL-8 in the supernatant and the expression of NF-kappaB P65 and MCP-1 mRNA of HUVECs were higher in TNF-alpha group and TNF-alpha + CIT group than those in the control group (P < 0.05), and those of TNF-alpha + CIT group was higher than TNF-alpha group (P < 0.05).
  
  
  CONCLUSIONS
  The expression of NF-kappaB, MCP-1, IL-1beta, IL-6 and IL-8 in HUVECs up-regulated by TNF-alpha was promoted by CIT.
authors.affiliation: Taishan Medical University,Tai'an 271000, China. hfhou@163.com
authors.forename: Haifeng
authors.initials: H
authors.lastname: Hou
authors.forename: Qiang
authors.initials: Q
authors.lastname: Jia
authors.forename: Ru
authors.initials: R
authors.lastname: Zhou
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Yuan
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Yang
authors.forename: Guoyong
authors.initials: G
authors.lastname: Ding
mesh_terms: D001313:Aurovertins / Q000494:pharmacology*; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D001313:Aurovertins; C111835:CCL2 protein, human; C508606:CXCL8 protein, human; D018932:Chemokine CCL2; C508585:IL1B protein, human; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D007378:Interleukins; C498143:RELA protein, human; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; C014416:citreoviridin