title: Development of experimental autoimmune encephalomyelitis (EAE) in mice requires vitamin D and the vitamin D receptor.
abstract: The development of experimental autoimmune encephalomyelitis (EAE), a model of multiple sclerosis, has been studied in mice that were (i) vitamin D-deficient, (ii) minus the vitamin D receptor, (iii) minus a vitamin D 25-hydroxylase, and (iv) minus the vitamin D 25-hydroxyvitamin D-1α-hydroxylase. EAE development was markedly suppressed in mice lacking the vitamin D receptor and partially suppressed in vitamin D-insufficient mice. However, the absence of either of the two key hydroxylases (i.e., 25-hydroxylase and 1α-hydroxylase) neither inhibits nor enhances the development of EAE. These results indicate that vitamin D and the vitamin D receptor are required for the development of EAE. The results also suggest that 1,25-dihydroxyvitamin D(3) may not play a role in this autoimmune response.
authors.affiliation: Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Marling
authors.forename: Jinge G
authors.initials: JG
authors.lastname: Zhu
authors.forename: Kyle S
authors.initials: KS
authors.lastname: Severson
authors.forename: Hector F
authors.initials: HF
authors.lastname: DeLuca
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D002118:Calcium / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism; D053493:Cholestanetriol 26-Monooxygenase / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments / Q000276:immunology; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism*; D014807:Vitamin D / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D014808:Vitamin D Deficiency / Q000097:blood / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D018167:Receptors, Calcitriol; C445081:myelin oligodendrocyte glycoprotein (35-55); D014807:Vitamin D; C569548:Cyp2r1 protein, mouse; D053493:Cholestanetriol 26-Monooxygenase; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; D002118:Calcium