title: The antioxidant activity of allylpyrocatechol is mediated via decreased generation of free radicals along with escalation of antioxidant mechanisms.
abstract: Allylpyrocatechol (APC) is responsible for the antiinflammatory activity exhibited by the methanolic extract of leaves of Piper betle. As antiinflammatory compounds may display antioxidant properties and vice versa, we investigated the antioxidant effect of APC. APC effectively reduced phorbol-myristate-acetate-induced generation of reactive oxygen species and superoxide in murine peritoneal macrophages as well as inhibited Escherichia-coli-induced phagocytic activity of macrophages. Furthermore, pBluescript SK(+) plasmid DNA damage induced by addition of sodium ascorbate was attenuated by APC as it inhibited transformation of the supercoiled form to a relaxed form. In addition, APC increased the enzymatic (catalase) and nonenzymatic (GSH) antioxidant components of murine macrophages. Taken together, APC exhibited an antioxidant activity which was mediated both via decreased generation of free radicals along with increase in cellular antioxidants.
authors.affiliation: Department of Pharmacology, Institute of Postgraduate Medical Education & Research, 244 B Acharya JC Bose Road, Kolkata, 700020, India.
authors.forename: Debjani
authors.initials: D
authors.lastname: Sarkar
authors.forename: Sunanda
authors.initials: S
authors.lastname: Kundu
authors.forename: Soumita
authors.initials: S
authors.lastname: De
authors.forename: Chellaram
authors.initials: C
authors.lastname: Hariharan
authors.forename: Piu
authors.initials: P
authors.lastname: Saha
authors.forename: Alak
authors.initials: A
authors.lastname: Manna
authors.forename: Subrata
authors.initials: S
authors.lastname: Chattopadhyay
authors.forename: Mitali
authors.initials: M
authors.lastname: Chatterjee
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D004278:DNA, Superhelical / Q000187:drug effects; D005609:Free Radicals / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D031721:Piper betle / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002396:Catechols; D004278:DNA, Superhelical; D005609:Free Radicals; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C523074:allylpyrocatechol; D002374:Catalase; D005978:Glutathione