title: Prognostic importance of VEGF-A haplotype combinations in a stage II colon cancer population.
abstract: OBJECTIVE
  To investigate the prognostic effect of three VEGF-A SNPs, -2578, -460 and 405, as well as the corresponding haplotype combinations, in a unique population of stage II colon cancer patients.
  
  
  METHODS
  The study included all patients diagnosed with stage II colon cancer in Denmark in 2003 (698 patients). One paraffin-embedded tissue block from each patient was used for DNA extraction and analysis of the three VEGF SNPs.
  
  
  RESULTS
  The homozygous genotype VEGF -2578 AA had significant effect on time to tumor recurrence (hazard ratio [HR] = 2.01 [95% CI: 1.13-3.56]; p = 0.02) as well as -460TT (HR = 0.50 [95% CI: 0.29-0.89]; p = 0.02). Patients harboring the haplotype combinations ACG,CTC and ACG,ACG displayed a significantly shorter time to tumor recurrence in both univariate (HR = 1.87 [95% CI: 1.21-2.89]; p = 0.008) and multivariate analysis (HR = 1.76 [95% CI: 1.09-2.82]; p = 0.02).
  
  
  CONCLUSIONS
  We found that the gene polymorphism in VEGF-A holds prognostic information and should be considered as a potential adjunct in identification of high-risk stage II colon cancer patients.
authors.affiliation: Danish Colorectal Cancer Group South, Vejle Hospital, Vejle, Denmark. sanne.kjaer.frifeldt@slb.regionsyddanmark.dk
authors.forename: Sanne
authors.initials: S
authors.lastname: Kjaer-Frifeldt
authors.forename: Rikke
authors.initials: R
authors.lastname: Fredslund
authors.forename: Jan
authors.initials: J
authors.lastname: Lindebjerg
authors.forename: Torben Froestrup
authors.initials: TF
authors.lastname: Hansen
authors.forename: Karen-Lise Garm
authors.initials: KL
authors.lastname: Spindler
authors.forename: Anders
authors.initials: A
authors.lastname: Jakobsen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D003718:Denmark; D005260:Female; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006720:Homozygote; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000473:pathology; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A