title: Antibacterial activity of selected Cameroonian dietary spices ethno-medically used against strains of Mycobacterium tuberculosis.
abstract: BACKGROUND
  Tuberculosis (TB) is considered as a re-emerging disease and one of the most important public health problems worldwide. The use or (in most cases) misuse of existint anti-tuberculosis drugs over the years has led to an increasing prevalence of resistant strains, establishing an urgent need to search for new effective agents. Spices are largely used ethno-medically across Africa.
  
  
  OBJECTIVE
  The present study aimed to evaluate the in vitro antimycobacterial activities of a total of 20 methanol crude extracts prepared from 20 Cameroonian dietary spices for their ability to inhibit the growth of or kill Mycobacterium tuberculosis strains H(37)Rv (ATCC 27294) and H(37)Ra (ATCC 25177).
  
  
  METHODS
  The antituberculosis screening was performed using the Microplate Alamar Blue Assay (MABA) method to determine the minimum inhibitory concentration (MIC) and the minimum mycobactericidal concentration (MBC).
  
  
  RESULTS
  Fifteen (15) plant extracts out of 20 showed varied levels of antimycobacterial activity against the strains M. tuberculosis H(37)Rv and H(37)Ra, with MICs in the range of 2.048-0.016 mg/ml. The extract of Echinops giganteus exhibited the most significant activity with a MIC value of 32 μg/ml and 16 μg/ml, respectively against H(37)Ra and H(37)Rv. To the best of our knowledge, the antimycobacterial activity of the tested spices has not been reported before and therefore our results can be evaluated as the first report about the antimycobacterial properties.
  
  
  CONCLUSIONS
  The results of this study suggest that Echinops giganteus and Piper guineense could be important sources of bactericidal compounds against M. tuberculosis and could probably be promising candidates that can be further investigated.
authors.affiliation: Laboratory for Tuberculosis Research, Biotechnology Centre, University of Yaoundé I, Yaoundé, PO Box: 812 Yaoundé, Cameroon. etekwu@yahoo.fr
authors.forename: Emmanuel Mouafo
authors.initials: EM
authors.lastname: Tekwu
authors.forename: Tülin
authors.initials: T
authors.lastname: Askun
authors.forename: Victor
authors.initials: V
authors.lastname: Kuete
authors.forename: Augustin Ephraim
authors.initials: AE
authors.lastname: Nkengfack
authors.forename: Barthélémy
authors.initials: B
authors.lastname: Nyasse
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Etoa
authors.forename: Véronique Penlap
authors.initials: VP
authors.lastname: Beng
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology* / Q000627:therapeutic use; D019659:Asteraceae; D002163:Cameroon; D004032:Diet; D006801:Humans; D019684:Magnoliopsida; D008519:Medicine, Traditional; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects*; D008517:Phytotherapy; D031701:Piper; D028321:Plant Preparations / Q000494:pharmacology* / Q000627:therapeutic use; D013045:Species Specificity; D017365:Spices; D014376:Tuberculosis / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D028321:Plant Preparations