title: Fabrication of pillared PLGA microvessel scaffold using femtosecond laser ablation.
abstract: One of the persistent challenges confronting tissue engineering is the lack of intrinsic microvessels for the transportation of nutrients and metabolites. An artificial microvascular system could be a feasible solution to this problem. In this study, the femtosecond laser ablation technique was implemented for the fabrication of pillared microvessel scaffolds of polylactic-co-glycolic acid (PLGA). This novel scaffold facilitates implementation of the conventional cell seeding process. The progress of cell growth can be observed in vitro by optical microscopy. The problems of becoming milky or completely opaque with the conventional PLGA scaffold after cell seeding can be resolved. In this study, PLGA microvessel scaffolds consisting of 47 μm × 80 μm pillared branches were produced. Results of cell culturing of bovine endothelial cells demonstrate that the cells adhere well and grow to surround each branch of the proposed pillared microvessel networks.
authors.affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Hsiao-Wei
authors.initials: HW
authors.lastname: Wang
authors.forename: Chung-Wei
authors.initials: CW
authors.lastname: Cheng
authors.forename: Ching-Wen
authors.initials: CW
authors.lastname: Li
authors.forename: Han-Wei
authors.initials: HW
authors.lastname: Chang
authors.forename: Ping-Han
authors.initials: PH
authors.lastname: Wu
authors.forename: Gou-Jen
authors.initials: GJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation*; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D004867:Equipment Design; D019344:Lactic Acid / Q000737:chemistry*; D007834:Lasers; D055616:Microtechnology / Q000379:methods; D055806:Microvessels; D008955:Models, Cardiovascular; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid