title: The significant increase of FcγRIIIA (CD16), a sensitive marker, in patients with coronary heart disease.
abstract: Our previous studies suggest that Fc receptor III A of immunoglobulin G (FcγRIIIA, also named CD16) is closely correlated to coronary heart disease (CHD). However, whether or not deregulated FcγRIIIA expression is involved in the development of CHD remains largely unclear. Herein, we investigated the FcγRIIIA mRNA expression in the leukocytes, the serum protein level of soluble CD16 (sCD16) and membrane CD16 on monocytes from 100 diagnosed CHD patients and 40 healthy individuals. Our results demonstrated that there was a significant increase of FcγRIIIA at the mRNA level in leukocytes, and at the protein level for both sCD16 in sera and membrane CD16 on monocytes from CHD patients compared to the healthy control. Similarly to the soluble CD14 (sCD14), the level of macrophage colony stimulating factor (M-CSF) in sera was also higher in CHD patients than that in the control individuals. Furthermore, the levels of inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1), in sera and the mean fluorescent intensity of intercellular adhesion molecule 1 (ICAM-1, CD54) on CD14(+) CD16(+) monocytes were increased in CHD patients. Overall, these data demonstrated that FcγRIIIA (CD16) is involved in the pathogenesis of CHD by activating monocytes and stimulating inflammation. The significant increase of CD14(+) CD16(+) monocytes in CHD patients therefore suggested that the increase of the FcγRIIIA level might be a sensitive marker for the CHD diagnosis.
authors.affiliation: Department of Cardiovascular Disease, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Huang
authors.forename: Huijun
authors.initials: H
authors.lastname: Yin
authors.forename: Jingshang
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Ma
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Keji
authors.initials: K
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D003327:Coronary Disease / Q000378:metabolism*; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D058851:GPI-Linked Proteins / Q000378:metabolism; D006801:Humans; D017452:Receptors, IgG / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D017931:DNA Primers; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D017452:Receptors, IgG