title: [Evaluation of the efficiency of differential diagnosis of influenza by multiplex real-time polymerase chain reaction].
abstract: The paper gives the results of a comparative analysis of the detection of influenza viruses in clinical samples, by using multiplex real-time polymerase chain reaction (RT-PCR) and by virus isolation in MDCK cell cultures. The investigation employed 267 nasopharyngeal swab specimens obtained from patients with influenza symptoms during two epidemic seasons (2008-2009 and 2009-2010). Influenza viruses were found in 104 samples (48 with influenza A virus (IAV) and 56 with influenza B virus (IBV)) by multiplex RT-RCR and in 84 samples (35 with IAV and 49 with IBV) by a cultural technique. The results of detection of influenza viruses by the two methods showed 89.4% agreement. The diagnostic sensitivity of multiplex RT-PCR testing a panel of the clinical samples in question was estimated to be 94.3% for IAV and 95.9% for IBV. The diagnostic sensitivity of multiplex RT-PCR in virus detection was demonstrated to be not only highly competitive with virus isolation, but also superior to the latter.
authors.forename: S A
authors.initials: SA
authors.lastname: Lobodanov
authors.forename: A A
authors.initials: AA
authors.lastname: Nikonova
authors.forename: E B
authors.initials: EB
authors.lastname: Faĭzuloev
authors.forename: S V
authors.initials: SV
authors.lastname: Trushakova
authors.forename: Iu I
authors.initials: IuI
authors.lastname: Zabiiaka
authors.forename: M A
authors.initials: MA
authors.lastname: Kalinkina
authors.forename: V T
authors.initials: VT
authors.lastname: Ivanova
authors.forename: E S
authors.initials: ES
authors.lastname: Shevchenko
authors.forename: V V
authors.initials: VV
authors.lastname: Zverev
authors.forename: E I
authors.initials: EI
authors.lastname: Burtseva
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D003937:Diagnosis, Differential; D004285:Dogs; D005260:Female; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification; D009981:Influenza B virus / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D008297:Male; D009305:Nasopharynx / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity