title: A molecular epidemiologic study of human adenovirus type 8 isolates causing epidemic keratoconjunctivitis in Kawasaki City, Japan in 2011.
abstract: A local outbreak of epidemic keratoconjunctivitis (EKC) caused by human  adenovirus type 8 (HAdV-D8) occurred in Kawasaki city, Japan in July-August 2011. Since the  cases were sporadic in nature, the source of the infection could not be identified. The results  of PCR analysis and the appearance of cytopathic effects in the samples indicated that 22  patients were positive for HAdV. The mean age of the patients (10 men and 12 women) was 64.3  ± 17.3 years (median, 68 years; range, 11-86 years). The sequences of hexon, which included  hypervariable loop 1; the penton, which included RGD loops; and the fiber, which included the  knob-coding regions, were identical in all the HAdV-positive cases. Phylogenetic analysis of  the major capsid protein-encoding regions of HAdV confirmed that the isolates were HAdV-D8.  Although the incidence of HAdV-D8 outbreaks has decreased in Japan since 1997, the results of  our study imply that HAdV-D8 is still a causative agent for EKC outbreaks in Japan.
authors.affiliation: Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan. fujimo-t＠nih.go.jp
authors.forename: Tsuguto
authors.initials: T
authors.lastname: Fujimoto
authors.forename: Yuki
authors.initials: Y
authors.lastname: Matsushima
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ishimaru
authors.forename: Atsuko
authors.initials: A
authors.lastname: Kano
authors.forename: Etsuko
authors.initials: E
authors.lastname: Nakajima
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Adhikary
authors.forename: Nozomu
authors.initials: N
authors.lastname: Hanaoka
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Okabe
mesh_terms: D000258:Adenovirus Infections, Human / Q000453:epidemiology* / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics* / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D036022:Capsid Proteins / Q000235:genetics; D002460:Cell Line; D002648:Child; D003236:Conjunctivitis, Viral / Q000453:epidemiology* / Q000821:virology; D058872:Epidemics; D005260:Female; D006801:Humans; D007564:Japan / Q000453:epidemiology; D007637:Keratoconjunctivitis / Q000453:epidemiology* / Q000821:virology; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus; C043322:penton protein, adenovirus