title: Secondary metabolites from the root of Ehretia longiflora and their biological activities.
abstract: Bioassay-guided fractionation of the methanolic extract of the root of Ehretia longiflora (Boraginaceae) afforded eight compounds, ehretiquinone (1), ehretiolide (2), ehreticoumarin (3), ehretilactone A (4), ehretilactone B (5), ehretiamide (6), ehretine (7), and ehretiate (8), together with 12 known compounds (9-20). The relative configuration of 1 was determined by single crystal X-ray diffraction. Among the isolates, 1 and prenylhydroquinone (14) showed antitubercular activity against Mycobacterium tuberculosis strain H37Rv with MIC values of 25.0 and 26.2 μg/mL, respectively. Moreover, 1 exhibited inhibitory effects on N-formylmethionylleucylphenylalanine (fMLP)-induced superoxide production, with IC₅₀ value of 0.36±0.03μM.
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, ROC.
authors.forename: Yu-Chieh
authors.initials: YC
authors.lastname: Chien
authors.forename: Chu-Hung
authors.initials: CH
authors.lastname: Lin
authors.forename: Michael Y
authors.initials: MY
authors.lastname: Chiang
authors.forename: Hsun-Shuo
authors.initials: HS
authors.lastname: Chang
authors.forename: Chang-Hui
authors.initials: CH
authors.lastname: Liao
authors.forename: Ih-Sheng
authors.initials: IS
authors.lastname: Chen
authors.forename: Chien-Fang
authors.initials: CF
authors.lastname: Peng
authors.forename: Ian-Lih
authors.initials: IL
authors.lastname: Tsai
mesh_terms: D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000995:Antitubercular Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D028082:Boraginaceae / Q000378:metabolism*; D006801:Humans; D020128:Inhibitory Concentration 50; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D018517:Plant Roots / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000995:Antitubercular Agents; D010936:Plant Extracts; D013481:Superoxides