title: Diazotrophic Burkholderia species isolated from the Amazon region exhibit phenotypical, functional and genetic diversity.
abstract: Forty-eight Burkholderia isolates from different land use systems in the Amazon region were compared to type strains of Burkholderia species for phenotypic and functional characteristics that can be used to promote plant growth. Most of these isolates (n=46) were obtained by using siratro (Macroptilium atropurpureum - 44) and common bean (Phaseolus vulgaris - 2) as the trap plant species; two isolates were obtained from nodules collected in the field from Indigofera suffruticosa and Pithecellobium sp. The evaluated characteristics were the following: colony characterisation on "79" medium, assimilation of different carbon sources, enzymatic activities, solubilisation of phosphates, nitrogenase activity and antifungal activity against Fusarium oxysporium f. sp. phaseoli. Whole cell protein profiles, 16S rRNA, gyrB, and recA gene sequencing and multilocus sequence typing were used to identify the isolates. The isolates showed different cultural and biochemical characteristics depending on the legume species from which they were obtained. Except for one isolate from I. suffruticosa, all isolates were able to solubilise calcium phosphate and present nitrogenase activity under free-living conditions. Only one isolate from common beans, showed antifungal activity. The forty four isolates from siratro nodules were identified as B. fungorum; isolates UFLA02-27 and UFLA02-28, obtained from common bean plants, were identified as B. contaminans; isolate INPA89A, isolated from Indigofera suffruticosa, was a close relative of B. caribensis but could not be assigned to an established species; isolate INPA42B, isolated from Pithecellobium sp., was identified as B. lata. This is the first report of nitrogenase activity in B. fungorum, B. lata and B. contaminans.
authors.affiliation: Departamento de Biologia, Universidade Federal de Lavras, Campus UFLA, Lavras, Minas Gerais, Brazil. krisle00@yahoo.com.br
authors.forename: Krisle
authors.initials: K
authors.lastname: da Silva
authors.forename: Alice de Souza
authors.initials: Ade S
authors.lastname: Cassetari
authors.forename: Adriana Silva
authors.initials: AS
authors.lastname: Lima
authors.forename: Evie
authors.initials: E
authors.lastname: De Brandt
authors.forename: Eleanor
authors.initials: E
authors.lastname: Pinnock
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.forename: Fatima Maria de Souza
authors.initials: FM
authors.lastname: Moreira
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D015373:Bacterial Typing Techniques; D044822:Biodiversity; D001938:Brazil; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009586:Nitrogen Fixation; D010802:Phylogeny; D010944:Plants / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D011938:Rec A Recombinases; D027081:DNA Gyrase