title: Decalpenic acid induces early osteoblastic markers in pluripotent mesenchymal cells via activation of retinoic acid receptor γ.
abstract: Decalpenic acid is a natural small molecule previously isolated from the fermentation broth of fungi that induces early osteoblastic markers in pluripotent mesenchymal cells. Treatment of mouse pluripotent mesenchymal C3H10T1/2 cells with decalpenic acid gave rise to a morphological change similar to that induced by the treatment with retinoic acid, i.e. the cells adopted a more elongated spindle shape. Using a retinoic acid response element reporter and receptor activity assays, we show that decalpenic acid is a new retinoid with selectivity towards retinoic acid receptors γ and α. The induction of early osteoblastic markers by decalpenic acid was significantly inhibited by treatment with the retinoid antagonist, LE540, or with small interfering RNA-mediated knockdown of retinoic acid receptor γ. These results demonstrated that decalpenic acid induces early osteoblastic markers in pluripotent mesenchymal cells through activation of retinoic acid receptor γ.
authors.affiliation: Institute of Microbial Chemistry, Tokyo, 3-14-23 Kamiosaki, Tokyo 141-0021, Japan. sakamotos@bikaken.or.jp
authors.forename: Shuichi
authors.initials: S
authors.lastname: Sakamoto
authors.forename: Fukiko
authors.initials: F
authors.lastname: Kojima
authors.forename: Isao
authors.initials: I
authors.lastname: Momose
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawada
authors.forename: Hayamitsu
authors.initials: H
authors.lastname: Adachi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Nishimura
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002264:Carboxylic Acids / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D002460:Cell Line; D003984:Dibenzazepines / Q000494:pharmacology; D020869:Gene Expression Profiling; D020868:Gene Silencing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009281:Naphthalenes / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000819:agonists* / Q000235:genetics; D000097768:Retinoic Acid Receptor gamma
chemical_list: D015415:Biomarkers; D002264:Carboxylic Acids; D003984:Dibenzazepines; D009281:Naphthalenes; D018168:Receptors, Retinoic Acid; D000097768:Retinoic Acid Receptor gamma; C104183:LE 540; C574719:decalpenic acid