title: Identification of dimorphism-involved genes of Yarrowia lipolytica by means of microarray analysis.
abstract: Fungal dimorphism is the capacity of certain species of fungi to grow in the form of budding yeasts or mycelium depending on the environmental conditions. This characteristic is a complex phenomenon that involves modifications of the molecular machinery in response to different environmental signals. Through the use of microarrays, in this work we identified genes involved in the early stages of the yeast-to-mycelium transition of Yarrowia lipolytica induced by a shift in pH of the medium. As controls, yeast and mycelium monomorphic mutants were used, identifying by this mean a total of 61 upregulated and 165 downregulated genes specifically involved in dimorphism. Determination of the putative function of these genes was accomplished by means of BLAST analyses which showed that they were involved mainly in processes such as remodeling and biogenesis of the cell wall, membrane trafficking and N- or O-glycosylation. Some of these genes were identified by homology with Saccharomyces cerevisiae genes, and found to play a role during the dimorphic transition in both systems.
authors.affiliation: Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 629, CP. 36500, Irapuato, Gto, Mexico. amorales@ira.cinvestav.mx
authors.forename: Adán Topiltin
authors.initials: AT
authors.lastname: Morales-Vargas
authors.forename: Angel
authors.initials: A
authors.lastname: Domínguez
authors.forename: José
authors.initials: J
authors.lastname: Ruiz-Herrera
mesh_terms: D003470:Culture Media / Q000737:chemistry; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D025301:Hyphae / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D046228:Microarray Analysis; D025062:Yarrowia / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*
chemical_list: D003470:Culture Media