title: Characterization of the immune response induced by a commercially available inactivated bluetongue virus serotype 1 vaccine in sheep.
abstract: The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4(+), CD8(+), and WC1(+)), CD25(+) regulatory cells, or CD14(+) monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14(+) monocytes, CD25(+) regulatory cells, and CD8(+) cytotoxic T lymphocytes.
authors.affiliation: VISAVET Health Surveillance Centre and Animal Health Department, Veterinary Faculty, Complutense University of Madrid, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Pérez de Diego
authors.forename: Pedro José
authors.initials: PJ
authors.lastname: Sánchez-Cordón
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: de las Heras
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Sánchez-Vizcaíno
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D015703:Antigens, CD / Q000276:immunology; D001820:Bluetongue virus / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D012756:Sheep; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D015703:Antigens, CD; D014765:Viral Vaccines