title: Adhesion of MC3T3-E1 cells bound to dentin phosphoprotein specifically bound to collagen type I.
abstract: Dentin sialophosphoprotein (DSPP) is a member of the SIBLING (small integrin binding N-linked glycoprotein) family of proteins commonly found in mineralized tissues. Dentin phosphoprotein (DPP) is a naturally occurring subdomain of DSPP that contains the cell binding RGD sequence. Previously, the orientation and conformation of other SIBLING family members specifically bound to collagen I have been investigated with respect to their cell adhesion properties. In this study, the orientation of DPP under similar circumstances is examined, and the results are discussed relative to the previous investigations. Radiolabeled adsorption isotherms were developed for DPP adsorbing to both tissue culture polystyrene (TCPS) and collagen coated TCPS. Then, a MC3T3-E1 cell adhesion assay was performed on TCPS and collagen coated TCPS in the presence of identical amounts of adsorbed DPP. It was discovered that there was a significant difference in the number of bound cells on the TCPS and collagen coated TCPS, with a preference for TCPS. Furthermore, a cell inhibition assay was conducted to confirm that the cell binding that occurred was due to specific integrin interactions with the RGD sequence of DPP. These results suggest that the orientation of DPP, rather than its conformation, dictates the accessibility of the cell binding RGD domains of DPP and that the RGD sequence in DPP is less accessible when DPP is specifically bound to collagen. The results obtained in this study are in stark contrast to previous studies with related SIBLING proteins, and suggest that DPP does not play a key role in cell binding to the collagen matrix of developing bone.
authors.affiliation: Department of Chemical Engineering, University of Missouri, Columbia, Missouri 65211, USA.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Zurick
authors.forename: Chunlin
authors.initials: C
authors.lastname: Qin
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Bernards
mesh_terms: D016475:3T3 Cells; D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D024042:Collagen Type I / Q000737:chemistry / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D011137:Polystyrenes / Q000737:chemistry; D051381:Rats; D012795:Sialoglycoproteins / Q000737:chemistry / Q000378:metabolism*; D000099311:Dentin Sialophosphoprotein
chemical_list: D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D011137:Polystyrenes; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein