title: [Simultaneous determination of six antibiotics in disinfection products by high performance liquid chromatography tandem mass spectrometry].
abstract: OBJECTIVE
  To develop a method for determination of the Metronidazole, Chlortetracycline hydrochloride, Oxytetracycline dihydrate, Minocycline hydrochloride, Erythromycin and Tetracycline hydrochloride in disinfection products by high performance liquid chromatography tandem mass spectrometry(LC-MS/MS).
  
  
  METHODS
  Samples were extracted by methanol and filtered through 0.45 microm PTFE membrane filter, then analyzed by LC-MS/MS using Waters Symmetry C18 (2.1 mm x 150 mm, 3.5 microm) column in positive ion scan mode. The mobile phase was 5 mmol/L ammonium acetate, methanol and acetonitrile.
  
  
  RESULTS
  The linear range was 0-2000 ng/ml and the correlation coefficients were more than 0.998, the average recoveries ranged from 74.7% to 114% with the relative standard deviations between 1.6%-20.2%. The method was successfully used to detect the content of antibiotics in 115 disinfection products.
  
  
  CONCLUSIONS
  The method is simple, sensitive, selective and suitable for the analysis of residual content of antibiotics in cream formulations of disinfection products.
authors.affiliation: Institute of Environmental Health and Related Product Safety, China CDC, Beijing 100021, China. lingmo2008@163.com
authors.forename: Hui
authors.initials: H
authors.lastname: Fu
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Hu
authors.forename: Changming
authors.initials: C
authors.lastname: Ding
authors.forename: Shaobin
authors.initials: S
authors.lastname: Lin
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002751:Chlortetracycline / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004202:Disinfectants / Q000032:analysis*; D008795:Metronidazole / Q000032:analysis*; D008911:Minocycline / Q000032:analysis; D009824:Ointments / Q000032:analysis*; D010118:Oxytetracycline / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D004202:Disinfectants; D009824:Ointments; D008795:Metronidazole; D008911:Minocycline; D002751:Chlortetracycline; D010118:Oxytetracycline