title: Expression of the Parkinson's disease protein DJ-1 during the differentiation of neural stem cells.
abstract: DJ-1 is a key neuroprotective factor and its loss-of-function mutations cause an autosomal recessive, early-onset form of familial Parkinson's disease at the chromosomal PARK7 locus. However, the expression of DJ-1 during the differentiation of neural stem cells has not been fully elucidated. In this study, we investigated the expression of DJ-1 quantitatively using fluorescence immunocytochemistry and flow cytometry for differentiated neural stem cells from the cortex of the 14-day mouse embryos. We found that DJ-1 was co-expressed with the neuron-specific enolase and glial fibrillary acidic proteins, and also its expression was significantly increased in neurons and astrocytes with a prolonged differentiation period. These findings strongly suggest that DJ-1 is closely associated with the differentiation of neural stem cells.
authors.affiliation: National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, PR China.
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Pu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004622:Embryo, Mammalian; D005260:Female; D005434:Flow Cytometry; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D058953:Neural Stem Cells / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011247:Pregnancy; D000071617:Protein Deglycase DJ-1; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; C404267:PARK7 protein, rat; D000071617:Protein Deglycase DJ-1; D010751:Phosphopyruvate Hydratase