title: Polycistronic coexpression and nondenaturing purification of histone octamers.
abstract: Histone octamers are the basic building blocks of chromatin and are platforms for diverse genetic mechanisms. We report a simple method for preparing recombinant histone octamers by overexpressing all four histones from a single polycistronic vector followed by standard chromatography under native conditions. This approach reduces the time needed for the octamer preparation to a single day and should be applicable to making a variety of unmodified and modified histone octamers.
authors.affiliation: Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA.
authors.forename: Yoonjung
authors.initials: Y
authors.lastname: Shim
authors.forename: Ming-Rui
authors.initials: MR
authors.lastname: Duan
authors.forename: Xuejing
authors.initials: X
authors.lastname: Chen
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Smerdon
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Min
mesh_terms: D001419:Bacteria; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics*; D006657:Histones / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D020033:Protein Isoforms; D011994:Recombinant Proteins