title: Discrimination of reproductive forms of Thrips tabaci (Thysanoptera: Thripidae) by PCR with sequence specific primers.
abstract: In agriculture, although it is important to identify species of pest insects, the morphological identification is often difficult. DNA genotyping is useful for the identification of species in morphologically indiscriminable species. Thrips tabaci (Lindeman) can be divided into two reproductive forms (arrhenotoky and thelytoky, each of which different in pesticide resistance) but morphological discrimination is not possible. Here, we establish a simple method to discriminate the strains based on their mitochondrial DNA sequences. Phylogenetic analysis including the T. tabaci and congeneric species provided ancestor sequences of each strain of T tabaci. Based on the ancestor sequences, we developed a primer set that include strain specific primers on sense strand and common primer on anti sense strand. Using this primer set, the strains of 196 individuals of T. tabaci were successfully assigned to each ofgenotypic forms. As the phylogeny and ancestor sequences were based on worldwide samples, this method will work well on most populations around the world.
authors.affiliation: Laboratory of Animal Ecology, Department of Ecology and Systematics, Graduate School of Agriculture, Hokkaido University, N9W9 Kita-ku, Sapporo 060-8589, Japan. kobakaz@res.agr.hokudai.ac.jp
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Eisuke
authors.initials: E
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D006239:Haplotypes; D019476:Insect Proteins / Q000235:genetics; D007564:Japan; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012098:Reproduction; D061149:Thysanoptera / Q000145:classification / Q000235:genetics* / Q000502:physiology
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D019476:Insect Proteins; D003576:Electron Transport Complex IV