title: Development of microsatellite markers in Parakmeria nitida (Magnoliaceae).
abstract: OBJECTIVE
  We developed microsatellite makers for Parakmeria nitida to investigate its population structure and conservation genetics.
  
  
  RESULTS
  A total of 25 microsatellite primer pairs were developed using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, and polymorphism was assessed in three natural populations of P. nitida. Among these markers, 11 were monomorphic and 14 showed polymorphism.
  
  
  CONCLUSIONS
  These markers are potentially useful for future population genetic analyses of P. nitida and will serve as an important tool for conservation efforts.
authors.affiliation: Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, People's Republic of China.
authors.forename: Jia-Min
authors.initials: JM
authors.lastname: Li
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Li
authors.forename: Long-Qian
authors.initials: LQ
authors.lastname: Xiao
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D015810:Linkage Disequilibrium; D031566:Magnolia / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant