title: [Role of C5b-9 expression in skeletal muscle blood vessels in necrotizing myopathy].
abstract: OBJECTIVE
  To investigate the expression of C5b-9 in the skeletal muscle blood vessels in patients with necrotizing myopathy and explore its role in the pathogenesis of this disease.
  
  
  METHODS
  The expression of C5b-9 and MHC-I in the skeletal muscular fibers and blood vessels in 4 patients with necrotizing myopathy was detected using enzymohistochemistry and immunohistochemistry.
  
  
  RESULTS
  Focal or dispersive necrotic muscle fibers with obvious phagocytosis were observed in all the 4 patients. No inflammatory cell infiltration was found in the perimysium or perivascular regions. HE staining showed a decreased number of local small blood vessels, and the some small blood vessels showed thickened vascular walls. Immunohistochemistry detected prominent C5b-9 expression in the necrotic muscle fibers and the blood vessels, and diffuse strong C5b-9 expression was found in the vascular walls, vascular endothelial cells and the smooth muscle layer. No MHC-I deposition was detected in the muscular fibers and blood vessels.
  
  
  CONCLUSIONS
  C5b-9 contributes to the pathogenesis of necrotizing myopathy mediated by pathologies in the blood vessels.
authors.affiliation: Department of Neurology, General Hospital of PLA, Beijing, China. congluconglu@sina.com
authors.forename: Lu
authors.initials: L
authors.lastname: Cong
authors.forename: Chuanqiang
authors.initials: C
authors.lastname: Pu
authors.forename: Yanling
authors.initials: Y
authors.lastname: Mao
authors.forename: Jiexiao
authors.initials: J
authors.lastname: Liu
authors.forename: Xianghui
authors.initials: X
authors.lastname: Lu
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000368:Aged; D015938:Complement Membrane Attack Complex / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000098:blood supply*; D009135:Muscular Diseases / Q000097:blood* / Q000473:pathology*; D009336:Necrosis
chemical_list: D015938:Complement Membrane Attack Complex