title: Crystal structure of a complex of DNA with one AT-hook of HMGA1.
abstract: We present here for the first time the crystal structure of an AT-hook domain. We show the structure of an AT-hook of the ubiquitous nuclear protein HMGA1, combined with the oligonucleotide d(CGAATTAATTCG)(2), which has two potential AATT interacting groups. Interaction with only one of them is found. The structure presents analogies and significant differences with previous NMR studies: the AT-hook forms hydrogen bonds between main-chain NH groups and thymines in the minor groove, DNA is bent and the minor groove is widened.
authors.affiliation: Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, Barcelona, Spain.
authors.forename: Elsa
authors.initials: E
authors.lastname: Fonfría-Subirós
authors.forename: Francisco
authors.initials: F
authors.lastname: Acosta-Reyes
authors.forename: Núria
authors.initials: N
authors.lastname: Saperas
authors.forename: Joan
authors.initials: J
authors.lastname: Pous
authors.forename: Juan A
authors.initials: JA
authors.lastname: Subirana
authors.forename: J Lourdes
authors.initials: JL
authors.lastname: Campos
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D024003:HMGA Proteins / Q000737:chemistry* / Q000378:metabolism; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D009841:Oligonucleotides / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D024003:HMGA Proteins; D009841:Oligonucleotides; D004247:DNA