title: Sexual hormones: effects on cardiac and mitochondrial activity after ischemia-reperfusion in adult rats. Gender difference.
abstract: In this work we studied the influence of sex hormones on heart and mitochondrial functions, from adult castrated female and male, and intact rats. Castration was performed at their third week of life and on the fourth month animals were subjected to heart ischemia and reperfusion. Electrocardiogram and blood pressure recordings were made, cytokines levels were measured, histopathological studies were performed and thiobarbituric acid reactive species were determined. At the mitochondrial level respiratory control, transmembranal potential and calcium management were determined; Western blot of some mitochondrial components was also performed. Alterations in cardiac function were worst in intact males and castrated females as compared with those found in intact females and castrated males, cytokine levels were modulated also by hormonal status. Regarding mitochondria, in those obtained from hearts from castrated females without ischemia-reperfusion, all evaluated parameters were similar to those observed in mitochondria after ischemia-reperfusion. The results show hormonal influences on the heart at functional and mitochondrial levels.
authors.affiliation: Departamento de Bioquímica, Instituto Nacional de Cardiología, Ignacio Chávez, Mexico, DF, Mexico. pavitonat@yahoo.com.mx
authors.forename: Natalia
authors.initials: N
authors.lastname: Pavón
authors.forename: Eduardo
authors.initials: E
authors.lastname: Martínez-Abundis
authors.forename: Luz
authors.initials: L
authors.lastname: Hernández
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Gallardo-Pérez
authors.forename: Carolina
authors.initials: C
authors.lastname: Alvarez-Delgado
authors.forename: Marco
authors.initials: M
authors.lastname: Cerbón
authors.forename: Israel
authors.initials: I
authors.lastname: Pérez-Torres
authors.forename: Alberto
authors.initials: A
authors.lastname: Aranda
authors.forename: Edmundo
authors.initials: E
authors.lastname: Chávez
mesh_terms: D000818:Animals; D002369:Castration; D045304:Cytochromes c / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004958:Estradiol / Q000097:blood; D005260:Female; D006321:Heart / Q000503:physiopathology*; D008297:Male; D008929:Mitochondria, Heart / Q000502:physiology*; D015428:Myocardial Reperfusion Injury / Q000097:blood / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011959:Receptors, Estradiol / Q000378:metabolism; D012727:Sex Characteristics; D013739:Testosterone / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D016207:Cytokines; D011959:Receptors, Estradiol; D017392:Thiobarbituric Acid Reactive Substances; D013739:Testosterone; D004958:Estradiol; D045304:Cytochromes c