title: Effects of sodium hydroxide exposure on esophageal epithelial cells in an in vitro ovine model: implications for esophagus tissue engineering.
abstract: BACKGROUND
  Esophagus tissue engineering holds promises for esophageal replacement after severe caustic injuries. The aim of this study was to determine whether viable esophageal epithelial cells could be isolated from an esophagus exposed to varying concentrations of alkali with regard to number, viability, and morphology during in vitro culture.
  
  
  METHODS
  Ovine esophagi were exposed to phosphate-buffered saline 2.5%, 15%, or 25% sodium hydroxide (NaOH). The effect of NaOH concentrations on epithelial damage was assessed histologically. Esophageal epithelial cells were then isolated, and cell count and viability were investigated. Finally, cell number, viability, and morphology of esophageal epithelial cells were determined for 24 days of in vitro culture.
  
  
  RESULTS
  Histologic analysis showed a progressive destruction of the epithelium proportional to increasing NaOH concentrations. Esophagi treated with phosphate-buffered saline and 2.5% NaOH showed significantly higher viable cell counts after isolation and culture in comparison with those treated with 15% to 5% NaOH.
  
  
  CONCLUSIONS
  The evidence presented in this study indicates that epithelial biopsies from an esophagus exposed to low concentrations (2.5%) of NaOH will still yield large numbers of viable cells suitable for tissue engineering applications. In cases of exposure to higher concentrations (15%-25%), alternative cell sources for epithelial regeneration, such as stem cells, will be necessary for tissue engineering applications.
authors.affiliation: Experimental Fetal Surgery and Tissue Engineering Unit, Department of Pediatric and Adolescent Surgery, Medical University of Graz, Auenbruggerplatz-34, A-8036 Graz, Austria.
authors.forename: Valeria
authors.initials: V
authors.lastname: Malvasio
authors.forename: Herwig
authors.initials: H
authors.lastname: Ainoedhofer
authors.forename: Richard
authors.initials: R
authors.lastname: Ackbar
authors.forename: Michael E
authors.initials: ME
authors.lastname: Hoellwarth
authors.forename: Amulya K
authors.initials: AK
authors.lastname: Saxena
mesh_terms: D000818:Animals; D001706:Biopsy; D002424:Caustics / Q000633:toxicity*; D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D004947:Esophagus / Q000187:drug effects* / Q000473:pathology; D012756:Sheep; D012972:Sodium Hydroxide / Q000633:toxicity*; D023822:Tissue Engineering
chemical_list: D002424:Caustics; D012972:Sodium Hydroxide