title: Biomass conversion determined via fluorescent cellulose decay assay.
abstract: An example of a rapid microtiter plate assay (fluorescence cellulose decay, FCD) that determines the conversion of cellulose in a washed biomass substrate is reported. The conversion, as verified by HPLC, is shown to correlate to the monitored FCD in the assay. The FCD assay activity correlates to the performance of multicomponent enzyme mixtures and is thus useful for the biomass industry. The development of an optimized setup of the 96-well microtiter plate is described, and is used to test a model that shortens the assay incubation time from 72 to 24h. A step-by-step procedure of the final assay is described.
authors.affiliation: Novozymes A/S, Bagsværd, Denmark.
authors.forename: Bente
authors.initials: B
authors.lastname: Wischmann
authors.forename: Marianne
authors.initials: M
authors.lastname: Toft
authors.forename: Marco
authors.initials: M
authors.lastname: Malten
authors.forename: K C
authors.initials: KC
authors.lastname: McFarland
mesh_terms: D018533:Biomass; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D057075:Enzyme Assays / Q000191:economics / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D008954:Models, Biological; D013050:Spectrometry, Fluorescence / Q000191:economics / Q000379:methods*; D013997:Time Factors; D003313:Zea mays / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D002482:Cellulose; D002480:Cellulase