title: Quantitative microscopy in murine models of lung inflammation.
abstract: OBJECTIVE
  To develop a quantitative means to measure lung inflammation using the murine models of chronic asthma and cystic fibrosis (CF).
  
  
  METHODS
  Translational-based medicine often utilizes animal models to study new and innovative therapeutics. In asthma and CF, the animal models focus on airway inflammation and remodeling. The asthma model is based on hypersensitivity-induced airway disease, whereas the CF model focuses on the inflammatory response to infection with Pseudomonas aeruginosa. Qualitative measures of inflammation and lung pathophysiology introduce significant variability and difficulty in interpreting interventional outcomes. The highly sensitive and reproducible quantitative computational program interfaced with Image Pro Microscopy to monitor changes in lung inflammation and lung pathophysiology. The software interfaces with image microscopy and automates the lung section review process.
  
  
  RESULTS
  Results from this program recapitulated data obtained by manual point counting of inflammation, bronchoalveolar lavage differential, and histology. The data show a low coefficient of variation and high reproducibility between slides and sections.
  
  
  CONCLUSIONS
  Utilization of this new microscopy program will enhance the quantitative means of establishing changes in lung structure and inflammation as a measure of therapeutic intervention with the ability of refining interpretation of in vivo models potentially short-circuiting translation into the clinical setting.
authors.affiliation: Hawken School, Gates Mills Ohio, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Soltzberg
authors.forename: Sarah
authors.initials: S
authors.lastname: Frischmann
authors.forename: Christiaan
authors.initials: C
authors.lastname: van Heeckeren
authors.forename: Nicole
authors.initials: N
authors.lastname: Brown
authors.forename: Arnold
authors.initials: A
authors.lastname: Caplan
authors.forename: Tracey L
authors.initials: TL
authors.lastname: Bonfield
mesh_terms: D000818:Animals; D001249:Asthma / Q000473:pathology*; D002908:Chronic Disease; D003550:Cystic Fibrosis / Q000473:pathology*; D004195:Disease Models, Animal; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007962:Leukocytes / Q000473:pathology; D008168:Lung / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008853:Microscopy / Q000379:methods*; D011014:Pneumonia / Q000473:pathology*; D012720:Severity of Illness Index; D012984:Software