title: A sensitive electrochemical biosensor for detection of DNA methyltransferase activity by combining DNA methylation-sensitive cleavage and terminal transferase-mediated extension.
abstract: A novel electrochemical biosensor was developed for activity assay of DNA methyltransferase and its inhibitor based on methylation-sensitive cleavage, which activated a primer for terminal transferase-mediated extension of biotinylated dUTP followed by sensitive detection via enzymatic amplification.
authors.affiliation: State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
authors.forename: Huiwang
authors.initials: H
authors.lastname: Wu
authors.forename: Shucheng
authors.initials: S
authors.lastname: Liu
authors.forename: Jianhui
authors.initials: J
authors.lastname: Jiang
authors.forename: Guoli
authors.initials: G
authors.lastname: Shen
authors.forename: Ruqin
authors.initials: R
authors.lastname: Yu
mesh_terms: D015374:Biosensing Techniques; D053837:DNA Cleavage / Q000187:drug effects; D019175:DNA Methylation / Q000187:drug effects; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D055664:Electrochemical Techniques; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D004791:Enzyme Inhibitors; D015254:DNA Modification Methylases