title: Genetic detection and characterization of atypical bovine pestiviruses in foetal bovine sera claimed to be of Australian origin.
abstract: Two European laboratories independently detected atypical bovine pestiviral nucleic acids in three commercial batches of foetal bovine serum (FBS) that was claimed by the producers to be of Australian origin. Additional batches of FBS were obtained directly from Australia to exclude possible contamination of the Australian FBS with that of South American origin during manufacturing/packaging in European countries. RT-PCR amplification of partial 5'untranslated region and the complete N(pro) gene yielded a specific band with expected size, which was confirmed by DNA sequencing. Bayesian analysis of sequence data demonstrated a closer phylogenetic relation of the newly detected atypical bovine pestiviruses to those of South American origin, which were related to the recognized bovine pestivirus species, bovine viral diarrhoea virus. Taken together, the results indicated the presence of atypical bovine pestiviruses in the Australian FBS, and most likely in Australian Continent.
authors.affiliation: Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Xia
authors.forename: M
authors.initials: M
authors.lastname: Larska
authors.forename: M
authors.initials: M
authors.lastname: Giammarioli
authors.forename: G M
authors.initials: GM
authors.lastname: De Mia
authors.forename: G
authors.initials: G
authors.lastname: Cardeti
authors.forename: W
authors.initials: W
authors.lastname: Zhou
authors.forename: S
authors.initials: S
authors.lastname: Alenius
authors.forename: S
authors.initials: S
authors.lastname: Belák
authors.forename: L
authors.initials: L
authors.lastname: Liu
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D001315:Australia; D001499:Bayes Theorem; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis* / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D001908:Diarrhea Viruses, Bovine Viral / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005312:Fetal Blood / Q000821:virology*; D016679:Genome, Viral; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000097:blood / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020121:5' Untranslated Regions; D012333:RNA, Messenger; D012367:RNA, Viral