title: A regulatory potential of the Xist gene promoter in vole M. rossiaemeridionalis.
abstract: X chromosome inactivation takes place in the early development of female mammals and depends on the Xist gene expression. The mechanisms of Xist expression regulation have not been well understood so far. In this work, we compared Xist promoter region of vole Microtus rossiaemeridionalis and other mammalian species. We observed three conserved regions which were characterized by computational analysis, DNaseI in vitro footprinting, and reporter construct assay. Regulatory factors potentially involved in Xist activation and repression in voles were determined. The role of CpG methylation in vole Xist expression regulation was established. A CTCF binding site was found in the 5' flanking region of the Xist promoter on the active X chromosome in both males and females. We suggest that CTCF acts as an insulator which defines an inactive Xist domain on the active X chromosome in voles.
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russia.
authors.forename: Konstantin E
authors.initials: KE
authors.lastname: Orishchenko
authors.forename: Sophia V
authors.initials: SV
authors.lastname: Pavlova
authors.forename: Eugeny A
authors.initials: EA
authors.lastname: Elisaphenko
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Sherstyuk
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Prinz
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Shevchenko
authors.forename: Elena V
authors.initials: EV
authors.lastname: Dementyeva
authors.forename: Suren M
authors.initials: SM
authors.lastname: Zakian
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D000076246:CCCTC-Binding Factor; D002460:Cell Line; D019175:DNA Methylation; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D008322:Mammals / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D050436:Regulatory Elements, Transcriptional; D012097:Repressor Proteins / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014960:X Chromosome / Q000235:genetics / Q000378:metabolism; D049951:X Chromosome Inactivation / Q000235:genetics*
chemical_list: D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D012097:Repressor Proteins; C077885:XIST non-coding RNA