title: Metabolic-intermediate complex formation with cytochrome P450: theoretical studies in elucidating the reaction pathway for the generation of reactive nitroso intermediate.
abstract: Mechanism-based inhibition (MBI) of cytochrome P450 (CYP) can lead to drug-drug interactions and often to toxicity. Some aliphatic and aromatic amines can undergo biotransformation reactions to form reactive metabolites such as nitrosoalkanes, leading to MBI of CYPs. It has been proposed that the nitrosoalkanes coordinate with the heme iron, forming metabolic-intermediate complex (MIC), resulting in the quasi-irreversible inhibition of CYPs. Limited mechanistic details regarding the formation of reactive nitroso intermediate and its coordination with heme-iron have been reported. A quantum chemical analysis was performed to elucidate potential reaction pathways for the generation of nitroso intermediate and the formation of MIC. Elucidation of the energy profile along the reaction path, identification of three-dimensional structures of reactive intermediates and transition states, as well as charge and spin density analyses, were performed using the density functional B3LYP method. The study was performed using Cpd I [iron (IV-oxo] heme porphine with SH(-) as the axial ligand) to represent the catalytic domain of CYP, simulating the biotransformation process. Three pathways: (i) N-oxidation followed by proton shuttle, (ii) N-oxidation followed by 1,2-H shift, and (iii) H-abstraction followed by rebound mechanism, were studied. It was observed that the proton shuttle pathway was more favorable over the whole reaction leading to reactive nitroso intermediate. This study revealed that the MIC formation from a primary amine is a favorable exothermic process, involving eight different steps and preferably takes place on the doublet spin surface of Cpd I. The rate-determining step was identified to be the first N-oxidation of primary amine.
authors.affiliation: Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research, S. A. S. Nagar, Mohali, Punjab, India.
authors.forename: Nikhil
authors.initials: N
authors.lastname: Taxak
authors.forename: Prashant V
authors.initials: PV
authors.lastname: Desai
authors.forename: Bhargav
authors.initials: B
authors.lastname: Patel
authors.forename: Michael
authors.initials: M
authors.lastname: Mohutsky
authors.forename: Valentine J
authors.initials: VJ
authors.lastname: Klimkowski
authors.forename: Vijay
authors.initials: V
authors.lastname: Gombar
authors.forename: Prasad V
authors.initials: PV
authors.lastname: Bharatam
mesh_terms: D000588:Amines / Q000737:chemistry / Q000378:metabolism; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D009603:Nitroso Compounds / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011789:Quantum Theory
chemical_list: D000588:Amines; D009603:Nitroso Compounds; D003577:Cytochrome P-450 Enzyme System