title: Nuage proteins: their localization in subcellular structures of spermatogenic cells as revealed by immunoelectron microscopy.
abstract: Chromatoid body (CB) was identified as granules stained by basic dye 130 years ago and called by various names. Electron microscopy revealed that the CB belonged to nuage (cloud in French) specific for germ cells. We described the localization of several proteins, including RNA helicases, in the nuage compartments classified into six types and in several spermatogenic cell-specific structures. All the proteins examined were detected in the nuage, including the CB with different staining intensities. Several proteins were localized to non-nuage structures, suggesting that these nuage proteins structures are related to nuage function.
authors.affiliation: Division of Functional Morphology, Faculty of Pharmaceutical Sciences, Nagasaki International University, Huis Ten Bosch 2825-7, Sasebo, Nagasaki 859-3298, Japan. syokota@niu.ac.jp
authors.forename: Sadaki
authors.initials: S
authors.lastname: Yokota
mesh_terms: D000818:Animals; D002842:Chromatids / Q000737:chemistry* / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D020365:RNA Helicases / Q000032:analysis*; D013091:Spermatogenesis
chemical_list: D020365:RNA Helicases