title: Increased initiation and growth of tumor cell lines, cancer stem cells and biopsy material in mice using basement membrane matrix protein (Cultrex or Matrigel) co-injection.
abstract: This protocol requires 2-4 h and presents a method for injecting tumor cells, cancer stem cells or dispersed biopsy material into subcutaneous or orthotopic locations within recipient mice. The tumor cells or biopsy are mixed with basement membrane matrix proteins (CultrexBME or Matrigel) at 4 °C and then injected into recipient animals at preferred anatomical sites. Tumor cells can also be co-injected with additional cell types, such as fibroblasts, stromal cells, endothelial cells and so on. Details are given on appropriate cell numbers, handling and concentration of the basement membrane proteins, recipient animals, injection location and techniques. This procedure enables the growth of tumors from cells or biopsy material (tumor graft) with greater efficiency of take and growth, and with retention of the primary tumor phenotype based on histology. Co-injection with additional cell types provides more physiological models of human cancers for use in drug screening and studying cancer biology.
authors.affiliation: Department of Pathology, Wayne State University School of Medicine, Karmanos Cancer Institute, Detroit, Michigan, USA. rfridman@med.wayne.edu
authors.forename: Rafael
authors.initials: R
authors.lastname: Fridman
authors.forename: Gabriel
authors.initials: G
authors.lastname: Benton
authors.forename: Irina
authors.initials: I
authors.lastname: Aranoutova
authors.forename: Hynda K
authors.initials: HK
authors.lastname: Kleinman
authors.forename: R Daniel
authors.initials: RD
authors.lastname: Bonfil
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000737:chemistry*; D001706:Biopsy; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D003094:Collagen; D004338:Drug Combinations; D006801:Humans; D007267:Injections; D007797:Laminin; D008175:Lung Neoplasms / Q000473:pathology; D008565:Membrane Proteins / Q000737:chemistry; D051379:Mice; D014411:Neoplastic Stem Cells / Q000473:pathology*; D011509:Proteoglycans; D055752:Small Cell Lung Carcinoma / Q000473:pathology; D023041:Xenograft Model Antitumor Assays / Q000379:methods*
chemical_list: D004338:Drug Combinations; D007797:Laminin; D008565:Membrane Proteins; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen