title: Oxidative stress-mediated apoptotic death in human peripheral blood lymphocytes treated with plasma from gamma-irradiated blood.
abstract: The present study is aimed to investigate the effect of plasma from irradiated human blood on the induction of apoptotic death in human peripheral blood lymphocytes. Our results showed that plasma obtained from γ-irradiated human blood showed a significant decrease in viability and an increase in apoptotic death in unirradiated lymphocytes, which is correlated with increase in intracellular reactive oxygen species level. Moreover, a decrease in the activity of superoxide dismutase in lymphocytes further seems to be associated with oxidative stress-mediated apoptotic death. The diffusible factors from irradiated blood were found to be generated from the cellular components of blood. Based on our results, we suggest induction of oxidative stress-mediated apoptotic death in unirradiated lymphocytes by diffusible factors from irradiated blood.
authors.affiliation: Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, India.
authors.forename: R
authors.initials: R
authors.lastname: Vasumathy
authors.forename: B N
authors.initials: BN
authors.lastname: Pandey
authors.forename: K P
authors.initials: KP
authors.lastname: Mishra
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D001769:Blood / Q000528:radiation effects*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D010949:Plasma; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase