title: In vivo protective effect of Copaifera langsdorffii hydroalcoholic extract on micronuclei induction by doxorubicin.
abstract: Copaifera lansdorffii Desf. is known as 'copaíba', 'copaiva' or 'paú-de-óleo', and is found in part of Brazil. The present study was undertaken to evaluate the genotoxic potential of C. langsdorffii leaf hydroalcoholic extract (CLE) and its influence on the genotoxicity induced by the chemotherapeutic agent doxorubicin (DXR) using the Swiss mouse peripheral blood micronucleus test. HPLC analysis of CLE using two monolithic columns linked in series allowed quantification of two major flavonoid heterosides, quercitrin and afzelin. Animals were treated with CLE by gavage at doses of 10, 20, 40 and 80 mg kg(-1) body weight per day, each for 20 days. Peripheral blood samples were collected at 24 and 48 h, and 7, 15 and 21 days after the beginning of the treatment. For the antigenotoxicity evaluation, the animals treated with different concentrations of CLE received DXR (15 mg kg(-1) body weight, intraperitoneal) at day 20. The peripheral blood samples were collected 24 and 48 h after the treatment with DXR. The results demonstrated that CLE itself was not genotoxic in the mouse micronucleus assay. In animals treated with CLE and DXR, the number of micronucleus was significantly decreased compared with animals receiving DXR alone. The putative antioxidant activity of one or more of the active compounds of CLE may explain the effect of this plant on DXR genotoxicity.
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
authors.forename: Jacqueline Morais
authors.initials: JM
authors.lastname: Alves
authors.forename: Carla Carolina
authors.initials: CC
authors.lastname: Munari
authors.forename: Moacir
authors.initials: M
authors.lastname: de Azevedo Bentes Monteiro Neto
authors.forename: Ricardo Andrade
authors.initials: RA
authors.lastname: Furtado
authors.forename: Juliana Marques
authors.initials: JM
authors.lastname: Senedese
authors.forename: Jairo Kenupp
authors.initials: JK
authors.lastname: Bastos
authors.forename: Denise Crispim
authors.initials: DC
authors.lastname: Tavares
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001938:Brazil; D004249:DNA Damage / Q000187:drug effects; D004317:Doxorubicin / Q000633:toxicity*; D007887:Fabaceae / Q000737:chemistry*; D008297:Male; D008362:Mannosides / Q000494:pharmacology; D051379:Mice; D015162:Micronucleus Tests; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D044945:Proanthocyanidins / Q000494:pharmacology; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D016587:Antimutagenic Agents; D008362:Mannosides; D010936:Plant Extracts; D044945:Proanthocyanidins; C012526:quercitrin; C477954:afzelin; D004317:Doxorubicin; D011794:Quercetin