title: Differences in H3K4 trimethylation in in vivo and in vitro fertilization mouse preimplantation embryos.
abstract: Trimethylation of lysine 4 at histone 3 (H3K4me3) is  considered a marker of active transcription; it plays an important role in  transcription reprogramming efficiency. We compared the levels of H3K4me3 in mouse  preimplantation embryos from MII stage oocytes produced by in vivo and in  vitro fertilization (IVF) using immunofluorescence histochemistry. IVF  embryos were further treated with trichostatin A (a histione deacetylase  inhibitor) to investigate the effect of histone acetylation on H3K4me3. We  found higher levels of H3K4me3 in MII stage oocytes in metaphase chromosomes.  The pattern of H3K4 trimethylation of in vivo embryos from zygote to  blastocyst stages was similar to that of IVF embryos; however, the  concentration of H3K4me3 was significantly higher in the in vivo fertilization  embryos. The levels of H3K4me3 in the trichostatin A-treated groups were also  significantly increased. We conclude that culture condition and environmental  changes can cause histone modification and that the effect of these  environmental conditions on epigenetic changes should be taken into  consideration.
authors.affiliation: School of Life Science, Fuyang Teachers College, Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province, Fuyang, China.
authors.forename: F-R
authors.initials: FR
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: M-B
authors.initials: MB
authors.lastname: Shang
authors.forename: X-X
authors.initials: XX
authors.lastname: Yang
authors.forename: B
authors.initials: B
authors.lastname: Ding
authors.forename: J-G
authors.initials: JG
authors.lastname: Gao
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.forename: W-Y
authors.initials: WY
authors.lastname: Li
mesh_terms: D000818:Animals; D001755:Blastocyst; D005260:Female; D005307:Fertilization in Vitro; D019084:Fluorescent Antibody Technique, Indirect; D006657:Histones / Q000378:metabolism*; D008745:Methylation; D051379:Mice
chemical_list: D006657:Histones