title: Formation of amyloid-like fibrils by Y-box binding protein 1 (YB-1) is mediated by its cold shock domain and modulated by disordered terminal domains.
abstract: YB-1, a multifunctional DNA- and RNA-binding nucleocytoplasmic protein, is involved in the majority of DNA- and mRNA-dependent events in the cell. It consists of three structurally different domains: its central cold shock domain has the structure of a β-barrel, while the flanking domains are predicted to be intrinsically disordered. Recently, we showed that YB-1 is capable of forming elongated fibrils under high ionic strength conditions. Here we report that it is the cold shock domain that is responsible for formation of YB-1 fibrils, while the terminal domains differentially modulate this process depending on salt conditions. We demonstrate that YB-1 fibrils have amyloid-like features, including affinity for specific dyes and a typical X-ray diffraction pattern, and that in contrast to most of amyloids, they disassemble under nearly physiological conditions.
authors.affiliation: Group of Protein Biosynthesis Regulation, Institute of Protein Research, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: Sergey G
authors.initials: SG
authors.lastname: Guryanov
authors.forename: Olga M
authors.initials: OM
authors.lastname: Selivanova
authors.forename: Alexey D
authors.initials: AD
authors.lastname: Nikulin
authors.forename: Gennady A
authors.initials: GA
authors.lastname: Enin
authors.forename: Bogdan S
authors.initials: BS
authors.lastname: Melnik
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Kretov
authors.forename: Igor N
authors.initials: IN
authors.lastname: Serdyuk
authors.forename: Lev P
authors.initials: LP
authors.lastname: Ovchinnikov
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009994:Osmolar Concentration; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014961:X-Ray Diffraction; D051840:Y-Box-Binding Protein 1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000682:Amyloid; D011994:Recombinant Proteins; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human