title: De novo sequencing and analysis of the termite mushroom (Termitomyces albuminosus) transcriptome to discover putative genes involved in bioactive component biosynthesis.
abstract: A one-eighth 454 sequencing run produced 82,386 high-quality reads. De novo assembly generated 6494 unique sequences. Based on the bioinformatic analysis, we found many the known enzymes involved in the biosynthesis of triterpene saponin in Termitomyces albuminosus, including 6 cytochrome P450 and 22 glycosyltransferase unique genes.
authors.affiliation: School of Life Science, Yunnan Normal University, Kunming 650500, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Sanjun
authors.initials: S
authors.lastname: Zhao
authors.forename: Junjun
authors.initials: J
authors.lastname: Li
authors.forename: Yunjuan
authors.initials: Y
authors.lastname: Yang
authors.forename: Xianghua
authors.initials: X
authors.lastname: Tang
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Mozhen
authors.initials: M
authors.lastname: Peng
authors.forename: Zunxi
authors.initials: Z
authors.lastname: Huang
mesh_terms: D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D020224:Expressed Sequence Tags; D036145:Ginsenosides / Q000096:biosynthesis / Q000737:chemistry; D016695:Glycosyltransferases / Q000235:genetics; D012503:Saponins / Q000096:biosynthesis*; D017422:Sequence Analysis, DNA; D055434:Termitomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics*; D014315:Triterpenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D036145:Ginsenosides; D012503:Saponins; D014315:Triterpenes; D003577:Cytochrome P-450 Enzyme System; D016695:Glycosyltransferases