title: Identification of nuclear localization signal in ASYMMETRIC LEAVES2-LIKE18/LATERAL ORGAN BOUNDARIES DOMAIN16 (ASL18/LBD16) from Arabidopsis.
abstract: The ASYMMETRIC LEAVES2-LIKE/LATERAL ORGAN BOUNDARIES DOMAIN (ASL/LBD) gene family encodes proteins harboring a conserved plant-specific LOB domain. The LOB domain contains a four-Cys motif, a Gly-Ala-Ser (GAS) block, and a Leu-zipper-like coiled-coil motif. The ASL/LBD proteins are a unique class of transcription factors that play roles in lateral organ development of plants. Although the ASL/LBD proteins are localized in the nucleus, no consensus sequence for the nuclear localization of the ASL/LBD proteins could be found. In the present study, we determined the motifs responsible for the nuclear localization of ASL18/LBD16 by using protoplast transfection assays with a variety N- or C-terminal deletion polypeptide fragments and the polypeptides harboring changes in basic amino acids that are fused to enhanced green fluorescent protein. The results demonstrated that ASL18/LBD16 harbors two distinct domains comprising an atypical nuclear localization signal (NLS) with basic amino acid residues in the coiled-coil motif and a monopartite-like NLS in the C-terminal region for nuclear targeting.
authors.affiliation: Department of Plant Biotechnology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Jungmook
authors.initials: J
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000737:chemistry; D002467:Cell Nucleus / Q000737:chemistry / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005810:Multigene Family; D019913:Nuclear Localization Signals / Q000737:chemistry / Q000235:genetics*; D018515:Plant Leaves; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000032:analysis / Q000737:chemistry
chemical_list: D029681:Arabidopsis Proteins; C573049:LBD18 protein, Arabidopsis; D019913:Nuclear Localization Signals; D014157:Transcription Factors