title: Detection and quantification of viral and satellite RNAs in plant hosts.
abstract: Northern blotting is a valuable method for detection and quantification of RNA in the field of virology. Although many methods including a various versions of polymerase chain reaction have been developed over the years, Northern blotting has been still considered as a useful and effective method for the analysis of progeny RNA accumulation for viral and subviral pathogens, such as satellite RNAs, in plant hosts. Here, we describe a detailed Northern blot protocol for efficient detection and quantification of viral and satellite RNAs from plant hosts.
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, CA, USA.
authors.forename: Sun-Jung
authors.initials: SJ
authors.lastname: Kwon
authors.forename: Jang-Kyun
authors.initials: JK
authors.lastname: Seo
authors.forename: A L N
authors.initials: AL
authors.lastname: Rao
mesh_terms: D015152:Blotting, Northern / Q000379:methods*; D017795:Bromovirus / Q000235:genetics*; D002021:Buffers; D019401:Cucumber Mosaic Virus Satellite / Q000235:genetics*; D017799:Cucumovirus / Q000235:genetics*; D003720:Densitometry; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005557:Formaldehyde / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization / Q000379:methods; D010761:Phosphorus Radioisotopes; D010944:Plants / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D013194:Staining and Labeling; D014158:Transcription, Genetic
chemical_list: D002021:Buffers; D019401:Cucumber Mosaic Virus Satellite; D010761:Phosphorus Radioisotopes; D012367:RNA, Viral; D005557:Formaldehyde