title: Indolepyruvate ferredoxin oxidoreductase: An oxygen-sensitive iron-sulfur enzyme from the hyperthermophilic archaeon Thermococcus profundus.
abstract: Thermococcus profundus is a strictly anaerobic sulfur-dependent archaeon that grows optimally at 80°C by peptide fermentation. Indolepyruvate ferredoxin oxidoreductase (IOR), an enzyme involved in the peptide fermentation pathway, was purified to homogeneity from the archaeon under strictly anaerobic conditions. The maximal activity was obtained above the boiling temperature of water (105°C), with a half-life of 62min at 100°C and 20min at 105°C. IOR was oxygen-sensitive with a half-life of 7h at 25°C under aerobic conditions. The specific activity of T. profundus IOR was found to be dependent on the number of [4Fe-4S] clusters in the enzyme.
authors.affiliation: Department of Life Science and Graduate School of Life Science, Rikkyo (St. Paul's) University, Toshima-ku, Tokyo 171-8501, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ozawa
authors.forename: Masood Ahmed
authors.initials: MA
authors.lastname: Siddiqui
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Akio
authors.initials: A
authors.lastname: Urushiyama
authors.forename: Daijiro
authors.initials: D
authors.lastname: Ohmori
authors.forename: Fumiyuki
authors.initials: F
authors.lastname: Yamakura
authors.forename: Fumio
authors.initials: F
authors.lastname: Arisaka
authors.forename: Takeo
authors.initials: T
authors.lastname: Imai
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D006207:Half-Life; D006358:Hot Temperature; D007501:Iron / Q000378:metabolism; D007658:Ketone Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D010100:Oxygen / Q000494:pharmacology; D013455:Sulfur / Q000378:metabolism; D019712:Thermococcus / Q000187:drug effects / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D013455:Sulfur; D007501:Iron; D007658:Ketone Oxidoreductases; C087685:indolepyruvate ferredoxin oxidoreductase; D010100:Oxygen