title: [The HPLC analysis of vanillil-almond, 5-hydroxiindolacetic, homovanillic and homogentisic acids in biologic fluids in clinical laboratory].
abstract: The simple and fast HPLC technique of analysis of vanillil-almond, 5-hydroxiindolacetic, homovanillic and homogentisic acids in urine with solid-phase extraction using ultra cross-linked polystyrene (Purosep-200) is proposed. The separation on column Chromolith Performance RP-18e "Merck" 100x4.6 mm with monolithic phase-reversed silica gel in isocratic or gradient mode with ultraviolet detection under 285 nm. The isocratic mode is applied in case of ordinary analysis of vanillil-almond or homogentisic acids. The composition of eluent is isopropanol - water - TFA (1:99:0.025, v/v/v), flow speed is 1400 mkl/min; pressure is 37 bar, full separation less than in 4 min. The gradient low pressure mode is applied to analyze vanillil-almond, hydroxiindolacetic and homovanillic acids. Under this approach the switching to second eluent occurred from second minute. The composition of eluent is isopropanol-water-TFA (6:94:0.025, v/v/v), flow speed is 1400 mkl/min, pressure is 43 bar, full separation is less than in 7 min. The output (extraction share) consisted 78-113%. The simplicity reproducibility and sufficient sensitivity of technique in combination with the possibility of its application on standard chromatographic equipment (isocratic pump and ultraviolet detector) made it useful for routine clinical application.
authors.forename: A A
authors.initials: AA
authors.lastname: Dutov
authors.forename: Iu L
authors.initials: IuL
authors.lastname: Luk'ianova
authors.forename: E V
authors.initials: EV
authors.lastname: Belen'kova
authors.forename: A V
authors.initials: AV
authors.lastname: Sverkunova
authors.forename: O N
authors.initials: ON
authors.lastname: Konovalova
authors.forename: E N
authors.initials: EN
authors.lastname: Fedorova
authors.forename: A V
authors.initials: AV
authors.lastname: Ermolina
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006713:Homogentisic Acid / Q000134:cerebrospinal fluid / Q000652:urine*; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid / Q000652:urine*; D006801:Humans; D012015:Reference Standards; D052616:Solid Phase Extraction; D014641:Vanillic Acid / Q000134:cerebrospinal fluid / Q000652:urine*
chemical_list: D014641:Vanillic Acid; D006713:Homogentisic Acid; D006719:Homovanillic Acid