title: Expression of complement component C7 and involvement in innate immune responses to bacteria in grass carp.
abstract: Activation of the complement system, which forms a major part of the innate immune system, results in the formation of the terminal complement complex. The complement component, C7, plays an integral role in the assembly of this complex within target cell membranes. In this study, C7 was isolated and characterized from grass carp, an important cultured fish in China. The predicted amino acid sequence of C7 cDNA (2644 bp) exhibited 55.4 and 48.3% homology with trout C7-1 and zebrafish C7, respectively. The grass carp C7 gene was consisted of 18 exons and 17 introns. C7 gene expression was detected in the trunk kidney, liver, head kidney, skin, spleen, heart and intestine. Significant changes in C7 transcript expression (>20-fold) were detected following Aeromonas hydrophila infection, indicating C7 involvement in innate immune responses to bacteria in teleost fish.
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China.
authors.forename: Yubang
authors.initials: Y
authors.lastname: Shen
authors.forename: Junbin
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xu
authors.forename: Jianjun
authors.initials: J
authors.lastname: Fu
authors.forename: Jiale
authors.initials: J
authors.lastname: Li
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D003184:Complement C7 / Q000235:genetics / Q000276:immunology; D005393:Fish Diseases / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary*; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D003184:Complement C7