title: Effect of low-level laser therapy (660 nm) on acute inflammation induced by tenotomy of Achilles tendon in rats.
abstract: In this study, we aimed to analyze the effects of low-level laser therapy (LLLT; 660 nm) on levels of protein expression of inflammatory mediators after cutting Achilles tendon of rats. Thirty Wistar male rats underwent partial incisions of the left Achilles tendon, and were divided into three groups of 10 animals according to the time of euthanasia after injury: 6, 24 and 72 h. Each group was then divided into control group and LLLT group (treated with 100 mW, 3.57 W cm(-2), 0.028 cm(2), 214 J cm(-2), 6 J, 60 s, single point). In LLLT group, animals were treated once time per day until the time of euthanasia established for each group. The group treated with LLLT showed a significant reduction of IL-1β compared with control groups at three time points (6 h: P=0.0401; 24 h: P=0.0015; 72 h: P=0.0463). The analysis of IL-6 showed significant reduction only in the LLLT group at 72 h compared with control group (P=0.0179), whereas IL-10 showed a significant increase in the treated group compared with control group at three experimental times (6 h: P=0.0007; 24 h: P=0.0256; 72 h: P<0.0001). We conclude that LLLT is an important modulator of inflammatory cytokines release after injury in Achilles tendon.
authors.affiliation: Post Graduate Program for Health and Development of the Central-West Region, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
authors.forename: Erica Martinho Salvador
authors.initials: EM
authors.lastname: Laraia
authors.forename: Iandara Schettert
authors.initials: IS
authors.lastname: Silva
authors.forename: Daniel Martins
authors.initials: DM
authors.lastname: Pereira
authors.forename: Filipe Abdalla
authors.initials: FA
authors.lastname: dos Reis
authors.forename: Regiane
authors.initials: R
authors.lastname: Albertini
authors.forename: Patrícia
authors.initials: P
authors.lastname: de Almeida
authors.forename: Ernesto Cesar Pinto
authors.initials: EC
authors.lastname: Leal Junior
authors.forename: Paulo
authors.initials: P
authors.lastname: de Tarso Camillo de Carvalho
mesh_terms: D000125:Achilles Tendon / Q000473:pathology*; D000818:Animals; D007249:Inflammation / Q000532:radiotherapy*; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013708:Tendon Injuries / Q000532:radiotherapy*