title: Adipose expression of adipocytokines in women with polycystic ovary syndrome.
abstract: OBJECTIVE
  To investigate the role of adipocytokines in the pathophysiology of polycystic ovary syndrome (PCOS) by analyzing the messenger RNA (mRNA) expression and plasma levels of adipocytokines.
  
  
  METHODS
  Cross sectional study.
  
  
  METHODS
  Hospital.
  
  
  METHODS
  Thirty-six women with PCOS, 17 lean (LP) and 19 obese (OP), and 24 age- and weight-matched controls, 8 lean (LC) and 16 obese (OC).
  
  
  METHODS
  Subcutaneous adipose tissue and fasting plasma samples collected from 60 women, and insulin sensitivity evaluated by euglycemic hyperinsulinemic clamp and homeostatic model assessment insulin resistance index (HOMA-IR).
  
  
  METHODS
  mRNA expression of adiponectin, leptin, and interleukin-6 (IL-6) in adipose tissue, and plasma levels of leptin, adiponectin, resistin, visfatin, and tumor necrosis factor α (TNF-α).
  
  
  RESULTS
  The baseline data on body mass index (BMI), age, androgen levels, and insulin sensitivity was published previously. We found no independent effect of PCOS on the adipose expression of leptin, adiponectin, or IL-6 or on the plasma levels of adiponectin, leptin, resistin, visfatin, and TNF-α. Obesity was associated with increased mRNA expression of leptin, lower expression of adiponectin, and increased plasma levels of leptin.
  
  
  CONCLUSIONS
  Obesity is per se associated with increased adipose expression and plasma levels of leptin, lower expression of adiponectin, and marginally elevated expression of IL-6, but PCOS does not appear to have an independent effect on the adipose expression of leptin, adiponectin, and IL-6 or the circulating adipocytokines.
  
  
  BACKGROUND
  NCT00975832.
authors.affiliation: Department of Obstetrics and Gynaecology, Copenhagen University, Hvidovre, Denmark. pernillefsvendsen@hotmail.com
authors.forename: Pernille Fog
authors.initials: PF
authors.lastname: Svendsen
authors.forename: Michael
authors.initials: M
authors.lastname: Christiansen
authors.forename: Paula Louise
authors.initials: PL
authors.lastname: Hedley
authors.forename: Lisbeth
authors.initials: L
authors.lastname: Nilas
authors.forename: Steen Bønløkke
authors.initials: SB
authors.lastname: Pedersen
authors.forename: Sten
authors.initials: S
authors.lastname: Madsbad
mesh_terms: D054392:Adipokines / Q000235:genetics* / Q000378:metabolism; D052242:Adiponectin / Q000097:blood / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000097:blood; D005260:Female; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics / Q000378:metabolism; D020738:Leptin / Q000097:blood / Q000235:genetics / Q000378:metabolism; D054409:Nicotinamide Phosphoribosyltransferase / Q000097:blood; D009765:Obesity / Q000150:complications / Q000235:genetics / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D052243:Resistin / Q000097:blood; D013851:Thinness / Q000150:complications / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: C499918:ADIPOQ protein, human; D054392:Adipokines; D052242:Adiponectin; D016207:Cytokines; D015850:Interleukin-6; D020738:Leptin; C499624:RETN protein, human; D012333:RNA, Messenger; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human