title: Protein quantification methods to determine protein concentration prior to electrophoresis.
abstract: During each step of a protein isolation technique, if enzyme activity is to be determined and before a protein mixture is separated on a polyacrylamide electrophoresis gel, it is important to determine the concentration of the protein(s) in solution. Measuring protein concentration involves absorbance in the UV range or staining the protein with dyes or copper. This review describes the various protein determination methods that can be employed to measure protein concentration in solution.
authors.affiliation: Biochemistry, University of KwaZulu-Natal, Scottsville, South Africa. goldringd@ukzn.ac.za
authors.forename: J P Dean
authors.initials: JP
authors.lastname: Goldring
mesh_terms: D004396:Coloring Agents / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011804:Quinolines / Q000737:chemistry; D012394:Rosaniline Dyes / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004396:Coloring Agents; D007202:Indicators and Reagents; D011506:Proteins; D011804:Quinolines; D012394:Rosaniline Dyes; C048139:Coomassie blue; D003300:Copper; C047117:bicinchoninic acid