title: Simultaneous spectrophotometric determination of paracetamol, phenylephrine and chlropheniramine in pharmaceuticals using chemometric approaches.
abstract: OBJECTIVE
  The linear multivariate calibration models such as principal components regression (PCR) and partial least squares regressions (PLS1 and PLS2) due to the mathematical simplicity and physical or chemical interpretability are sufficient and generally preferred method for analysis of multicomponent drugs. In this study, simultaneous determination of paracetamol, phenylephrine and chlorpheniramine in pharmaceuticals using chemometric methods and UV spectrophotometry is reported as a simple alternative technique.
  
  
  METHODS
  Principal components regression (PCR) and partial least squares regressions (PLS1 and PLS2) were used for chemometric analyses of data obtained from the spectra of paracetamol, phenylephrine and chlorpheniramine between wavelengths of 200 to 400 nm at several concentrations within their linear ranges. The analytical performance of these chemometric methods were characterized by relative prediction errors and recoveries (%) and compared with each other.
  
  
  RESULTS
  PCR, PLS1 and PLS2 were successfully applied to a tablet formulation, with no interference from excipients as indicated by the recovery. However, the PLS1 shows better results due to its flexibility and mathematical principals.
  
  
  CONCLUSIONS
  The proposed methods are simple and rapid requiring no separation step, and can be easily used as an alternative in the quality control of drugs.
authors.affiliation: Department of Drug and Food Control, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran.
authors.forename: M R
authors.initials: MR
authors.lastname: Khoshayand
authors.forename: H
authors.initials: H
authors.lastname: Abdollahi
authors.forename: A
authors.initials: A
authors.lastname: Ghaffari
authors.forename: M
authors.initials: M
authors.lastname: Shariatpanahi
authors.forename: H
authors.initials: H
authors.lastname: Farzanegan