title: Differential expression  analysis of porcine MDH1, MDH2 and ME1 genes in adipose  tissues.
abstract: Malate  dehydrogenases 1 and 2 (MDH1 and MDH2), and malic enzyme 1 (ME1)  play important roles in the Krebs cycle for energy metabolism. The mRNA  abundance changes of MDH1, MDH2 and ME1 genes were  measured across six different adipose tissues from the leaner Landrace and  fatty Rongchang pig breeds using quantitative real-time PCR. The mRNA of MDH1, MDH2 and ME1 was more abundant in fatty Rongchang pigs than in  leaner Landrace pigs. In both breeds, females exhibited higher adipocyte volume  and mRNA abundance of MDH1, MDH2 and ME1 compared with  males. These values were higher in the subcutaneous adipose tissue compared  with visceral adipose tissue. Furthermore, mRNA abundance changes of MDH1, MDH2 and ME1 have the remarked significant positive correlation  with adipocyte volume across the six adipose tissue types. We conclude that  there are breed-, gender- and tissue-specific expression patterns of ME1, MDH1 and MDH2, which highlight their potential as candidate genes  for selecting for fat volume in pigs.
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Sichuan, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Zhou
authors.forename: M Z
authors.initials: MZ
authors.lastname: Li
authors.forename: Q H
authors.initials: QH
authors.lastname: Li
authors.forename: J Q
authors.initials: JQ
authors.lastname: Guan
authors.forename: X W
authors.initials: XW
authors.lastname: Li
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D007527:Isoenzymes / Q000235:genetics*; D008291:Malate Dehydrogenase / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine
chemical_list: D017931:DNA Primers; D007527:Isoenzymes; D012333:RNA, Messenger; D008291:Malate Dehydrogenase