title: HLA-DRβ1*04 typing by simple in-house PCR-SSP technique for rheumatoid arthritis patients.
abstract: A strong association between rheumatoid arthritis (RA) and human leukocyte antigen (HLA) has been observed in many different populations and that accounts for approximately one-third of the genetic component of RA susceptibility. The greatest effect comes from DRβ1 gene where the strongest association has been found with DRβ1*04 (DR4) allele. As serology has some disadvantages over polymerase chain reaction (PCR)-based techniques and commercially available PCR-based kits are expensive, this study was aimed to standardize simple in-house PCR-SSP technique. Accuracy of this test was further checked with standard PCR-SSOP (RLS) results. The frequency HLA-DRβ1*04 was significantly increased among RA patients when compared with normal controls. In this study, a very simple, convenient and more cost-effective in-house PCR-SSP technique was standardized for HLA-DRβ1*04 typing that is helpful to RA diagnosis in developing countries like India, which can be used as a good screening test.
authors.affiliation: National Institute of Immunohaematology ICMR, Mumbai, Maharashtra, India.
authors.forename: Devaraj Jatteppa
authors.initials: DJ
authors.lastname: Parasannanavar
authors.forename: Avani
authors.initials: A
authors.lastname: Yeola
authors.forename: Vandana
authors.initials: V
authors.lastname: Pradhan
authors.forename: Anjali
authors.initials: A
authors.lastname: Rajyadhaksha
authors.forename: Kanjaksha
authors.initials: K
authors.lastname: Ghosh
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology; D005260:Female; D005838:Genotype; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D059811:HLA-DRB1 Chains; C527195:HLA-DRB1*04 antigen