title: Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3A.
abstract: In human skeletal muscle, myoblasts represent the main population of myogenic progenitors. We previously showed that, beside their myogenic differentiation capacities, myoblasts also differentiate towards osteogenic and chondrogenic lineages, some properties generally considered being hallmarks of mesenchymal stem cells (MSCs). MSCs are also characterized by their immunosuppressive potential, through cell-cell contacts and soluble factors, including prostaglandin E-2 (PGE-2), transforming growth factor-β1 (TGF-β1), interleukine-10, or indoleamine 2,3-dioxygenase. We and others also reported that Galectin-1 (Gal-1) and Semaphorin-3A (Sema-3A) were involved in MSCs-mediated immunosuppression. Here, we show that human myoblasts induce a significant and dose-dependant proliferation inhibition, independently of PGE-2 and TGF-β1. Our experiments revealed that myoblasts, in culture or in situ in human muscles, expressed and secreted Gal-1 and Sema-3A. Furthermore, myoblasts immunosuppressive functions were reverted by using blocking antibodies against Gal-1 or Sema-3A. Together, these results demonstrate an unsuspected immunosuppressive effect of myoblasts that may open new therapeutic perspectives.
authors.affiliation: INSERM UMR940, Institut Universitaire d'Hématologie, 75475 Paris Cedex 10, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Lecourt
authors.forename: Yves
authors.initials: Y
authors.lastname: Lepelletier
authors.forename: Valérie
authors.initials: V
authors.lastname: Vanneaux
authors.forename: Rafika
authors.initials: R
authors.lastname: Jarray
authors.forename: Thomas
authors.initials: T
authors.lastname: Domet
authors.forename: Françoise
authors.initials: F
authors.lastname: Raynaud
authors.forename: Réda
authors.initials: R
authors.lastname: Hadj-Slimane
authors.forename: Audrey
authors.initials: A
authors.lastname: Cras
authors.forename: Olivier
authors.initials: O
authors.lastname: Hermine
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Marolleau
authors.forename: Jérôme
authors.initials: J
authors.lastname: Larghero