title: Mast cells express tyrosine hydroxylase and store dopamine in a serglycin-dependent manner.
abstract: Here we show that mast cells contain dopamine and that mast cell activation causes dopamine depletion, indicating its presence within secretory granules. Dopamine storage increased during mast cell maturation from bone marrow precursors, and was dependent on the presence of serglycin. Moreover, the expression of tyrosine hydroxylase, the key enzyme in dopamine biosynthesis, was induced during mast cell maturation; histidine decarboxylase and tryptophan hydroxylase 1 were also induced. Mast cell activation caused a robust induction of histidine decarboxylase, but no stimulation of tyrosine hydroxylase or tryptophan hydroxylase 1 expression. The present study points toward a possible role of dopamine in mast cell function.
authors.affiliation: Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, S-75123 Uppsala, Sweden. Elin.Ronnberg@slu.se
authors.forename: Elin
authors.initials: E
authors.lastname: Rönnberg
authors.forename: Gabriela
authors.initials: G
authors.lastname: Calounova
authors.forename: Gunnar
authors.initials: G
authors.lastname: Pejler
mesh_terms: D000818:Animals; D004298:Dopamine / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D006640:Histidine Decarboxylase / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D011509:Proteoglycans / Q000172:deficiency / Q000378:metabolism*; D014365:Tryptophan Hydroxylase / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis* / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000172:deficiency / Q000378:metabolism*
chemical_list: D011509:Proteoglycans; D033921:Vesicular Transport Proteins; C075430:serglycin; D014446:Tyrosine 3-Monooxygenase; D014365:Tryptophan Hydroxylase; D006640:Histidine Decarboxylase; D004298:Dopamine