title: Matrix metalloproteinase 2 of grass carp Ctenopharyngodon idella (CiMMP2) is involved in the immune response against bacterial infection.
abstract: The gene encoding matrix metalloproteinase 2 (MMP2) was cloned from grass carp (Ctenopharyngodon idella), and its expression levels during Aeromonas hydrophila infection and embryonic development stages were evaluated. The complete open reading frame of CiMMP2 was 1974 bp in length, encoding a 658-amino acid polypeptide. The deduced MMP2 protein contained four conserved domain structures, including an N-terminal signal sequence, a propeptide domain, three repeats of fibronectin-type II domain inserted in the catalytic domain and a C-terminal hemopexin-like domain. Phylogenetic analysis of MMP2s grouped grass carp with other teleosts. Detected in all fish tissues examined, CiMMP2 expression increased in the spleen and head kidney at 4 h and was significantly downregulated at 1 d after A. hydrophila infection. CiMMP2 transcripts were present in unfertilized eggs, suggesting its maternal origin. These findings implicate an important role for CiMMP2 in A. hydrophila-related diseases and early embryonic developmental stages of grass carp.
authors.affiliation: Key Laboratory of Freshwater Fisheries Genetic Resources Certificated by Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Xu
authors.forename: Yu-Bang
authors.initials: YB
authors.lastname: Shen
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Fu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Shi-Zhao
authors.initials: SZ
authors.lastname: Guo
authors.forename: Xiao-Meng
authors.initials: XM
authors.lastname: Yang
authors.forename: Jia-Le
authors.initials: JL
authors.lastname: Li
mesh_terms: D016980:Aeromonas hydrophila; D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D005393:Fish Diseases / Q000201:enzymology* / Q000276:immunology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D016905:Gram-Negative Bacterial Infections / Q000201:enzymology* / Q000276:immunology; D060226:Head Kidney / Q000201:enzymology; D020778:Matrix Metalloproteinase 2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D013154:Spleen / Q000201:enzymology
chemical_list: D020778:Matrix Metalloproteinase 2