title: Cell motility, morphology, viability and proliferation in response to nanotopography on silicon black.
abstract: Knowledge of cells' interactions with nanostructured materials is fundamental for bio-nanotechnology. We present results for how individual mouse fibroblasts from cell line NIH3T3 respond to highly spiked surfaces of silicon black that were fabricated by maskless reactive ion etching (RIE). We did standard measurements of cell viability, proliferation, and morphology on various surfaces. We also analyzed the motility of cells on the same surfaces, as recorded in time lapse movies of sparsely populated cell cultures. We find that motility and morphology vary strongly with nano-patterns, while viability and proliferation show little dependence on substrate type. We conclude that motility analysis can show a wide range of cell responses e.g. over a factor of two in cell speed to different nano-topographies, where standard assays, such as viability or proliferation, in the tested cases show much less variation of the order 10-20%.
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads 345a, 2800 Kongens Lyngby, Denmark. joanna.lopacinska@gmail.com
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Łopacińska
authors.forename: Cristian
authors.initials: C
authors.lastname: Grădinaru
authors.forename: Rafal
authors.initials: R
authors.lastname: Wierzbicki
authors.forename: Carsten
authors.initials: C
authors.lastname: Købler
authors.forename: Michael S
authors.initials: MS
authors.lastname: Schmidt
authors.forename: Martin T
authors.initials: MT
authors.lastname: Madsen
authors.forename: Maciej
authors.initials: M
authors.lastname: Skolimowski
authors.forename: Martin
authors.initials: M
authors.lastname: Dufva
authors.forename: Henrik
authors.initials: H
authors.lastname: Flyvbjerg
authors.forename: Kristian
authors.initials: K
authors.lastname: Mølhave
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D051379:Mice; D041681:NIH 3T3 Cells; D049329:Nanostructures / Q000737:chemistry / Q000633:toxicity; D036103:Nanotechnology; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D012825:Silicon