title: Enhancement of the promiscuous aldolase and dehydration activities of 4-oxalocrotonate tautomerase by protein engineering.
abstract: Double play: The enzyme 4-oxalocrotonate tautomerase (4-OT) catalyzes not only the initial cross-coupling of acetaldehyde and benzaldehyde to yield 3-hydroxy-3-phenylpropanal, but also the subsequent dehydration of this aldol compound to yield cinnamaldehyde as the final product. Mechanism-inspired engineering provided an active site mutant (F50A) with strongly enhanced aldol condensation activity.
authors.affiliation: Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Ellen
authors.initials: E
authors.lastname: Zandvoort
authors.forename: Edzard M
authors.initials: EM
authors.lastname: Geertsema
authors.forename: Wim J
authors.initials: WJ
authors.lastname: Quax
authors.forename: Gerrit J
authors.initials: GJ
authors.lastname: Poelarends
mesh_terms: D055162:Biocatalysis; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D007535:Isomerases / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D015202:Protein Engineering; D013379:Substrate Specificity; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water; D005634:Fructose-Bisphosphate Aldolase; C021918:4-oxalocrotonate tautomerase; D007535:Isomerases