title: [Detection of matrix metalloproteinase-11 in menstrual blood by enhanced chemiluminescence method].
abstract: OBJECTIVE
  To explore the forensic application value of detection of matrix metalloproteinase-11 (MMP-11) in menstrual blood by enhanced chemiluminescence method.
  
  
  METHODS
  Menstrual blood, vaginal swab, peripheral blood, saliva stain, urine stain and semen stain were collected to detect whether or not there were MMP-11 using enhanced chemiluminescence method. The specificity and reliability of the MMP-11 assay along with its sensitivity were evaluated.
  
  
  RESULTS
  The positive detection rate of MMP-11 in menstrual blood was 89.47%, whereas no MMP-11 was found in vaginal swab, peripheral blood, saliva stain, urine stain and semen stain. When 25 microL sample was added, the mass concentration of protein was 1.329 microg/microL, then MMP-11 could be detected. A positive detection rate of 89.58% was observed in MMP-11 positive menstrual blood samples after stored at 4 degrees C for 20 months.
  
  
  CONCLUSIONS
  Enhanced chemiluminescence method is sensitive and specific for detecting MMP-11, and can be applied to distinguish menstrual blood from common stain such as peripheral blood, vaginal fluid.
authors.affiliation: Forensic Judical Appraisal Institute, Blood Center of Hainan Province, Haikou 570311, China. fywzzyq2005@yahoo.com.cn
authors.forename: Ya-Qing
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Lu
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Yao
mesh_terms: D015415:Biomarkers / Q000097:blood; D001801:Blood Stains; D015153:Blotting, Western; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D053507:Matrix Metalloproteinase 11 / Q000032:analysis / Q000097:blood*; D008598:Menstruation; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry; D012680:Sensitivity and Specificity; D014556:Urine / Q000737:chemistry; D014621:Vagina / Q000737:chemistry
chemical_list: D015415:Biomarkers; D053507:Matrix Metalloproteinase 11