title: Functional and heterologous expression of human protein O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 in zebrafish.
abstract: Although membrane-associated proteins are related to many diseases and are important targets for drug discovery, their expression is often difficult in bacterial hosts such as Escherichia coli. To overcome this limitation, here, we focused on a novel host-vector system in zebrafish for the expression of human protein O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 (hPOMGnT1) which is related to muscle-eye-brain disease. For the expression of hPOMGnT1, the vector pZex-EGFP-pXI-hPOMGnT1 was constructed and injected into fertilized eggs. Using this system, we demonstrated that recombinant hPOMGnT1 was successfully expressed in the whole bodies of zebrafish embryos.
authors.affiliation: Department of Life Sciences, Graduate School of Bioresources, Mie University, Tsu, Mie, Japan. 508d301@m.mie-u.ac.jp
authors.forename: Eriko
authors.initials: E
authors.lastname: Avşar-Ban
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Shin'ichi
authors.initials: S
authors.lastname: Akiyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Manya
authors.forename: Tamao
authors.initials: T
authors.lastname: Endo
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamaru
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D017351:N-Acetylglucosaminyltransferases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D017351:N-Acetylglucosaminyltransferases; C443106:protein O-mannose beta-1,2-N-acetylglucosaminyltransferase