title: Development of multiplex PCR system with 15 X-STR loci and genetic analysis in three nationality populations from China.
abstract: The aim of this study is to develop a new multiplex PCR system that simultaneously amplifies the 15 X-chromosome short tandem repeats (X-STRs) loci in the same PCR reaction, and to obtain the 15 X-STR loci database in three nationality populations from China. This multiplex system includes DXS7133, DXS6801, DXS981, DXS6809, DXS7424, DXS6789, DXS9898, DXS7132, GATA165B12, DXS101, DXS10075, DXS6800, GATA31E08, DXS10074, and DXS10079, which were successfully analyzed on 1251 DNA samples (670 males and 581 females) from Guangdong Han population, Xinjiang Uigur and Kazakh. The allele frequencies and mutation rates of the 15 loci were investigated, and the allele frequency distribution among different populations was compared. A total of 6-17 alleles for each locus were observed and altogether 170 alleles for all the selected loci were found. Thirteen cases with mutation of the above loci were detected in 11,850 meioses. Pairwise comparisons of the allele frequencies distribution showed significant differences in most loci among different populations. The results indicate that this multiplex system may provide high polymorphism information for kinship testing and relationship investigations, and it is necessary to gain allele frequency and mutation rate of different population for forensic application.
authors.affiliation: Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, P R China.
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Liu
authors.forename: De-Jian
authors.initials: DJ
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Quan
authors.forename: Ye-Fei
authors.initials: YF
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Shen
authors.forename: Hu
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000704:Analysis of Variance; D044466:Asian People / Q000235:genetics*; D002681:China; D041321:Chromosomes, Human, X; D005190:Family; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009154:Mutation; D011110:Polymorphism, Genetic; D012680:Sensitivity and Specificity
chemical_list: D005819:Genetic Markers