title: Continuous chelation irrigation improves the adhesion of epoxy resin-based root canal sealer to root dentine.
abstract: OBJECTIVE
  To test the impact of continuous chelation by NaOCl+ etidronic acid (HEBP) during instrumentation, and a final rinse of EDTA or NaOCl + HEBP on the dentine bond strength of an epoxy resin sealer (AH Plus).
  
  
  METHODS
  Single-rooted teeth (n = 100) were divided into five groups (n = 20) based on the irrigation protocol and their root canals instrumented using a rotary Ni-Ti system: 2.5% NaOCl during instrumentation followed by bi-distilled water (G1) or 17% EDTA (G2) as final rinse; 1 : 1 mixture of 5% NaOCl and 18% HEBP during instrumentation, and the same mixture (G3), 17% EDTA (G4) or bi-distilled water (G5) as final rinse. Canals were filled with AH Plus. Roots were sectioned, and push-out tests were performed in coronal, middle and apical root thirds. Results were analysed using analysis of variance (anova) and Bonferroni test for multiple comparisons. The alpha-type error was set at 0.05 for all the analyses.
  
  
  RESULTS
  Push-out bond strength was highest in coronal and lowest in apical root thirds (P < 0.05). Groups that used NaOCl + HEBP irrigation during instrumentation had significantly higher bond strengths than groups following the NaOCl-EDTA irrigation in all root thirds (P < 0.05). The use of a strong chelator as final flush further increased bond strengths (G4, P < 0.05).
  
  
  CONCLUSIONS
  The continuous chelation irrigation protocol optimizes the bond strength of an epoxy resin sealer to dentine.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha University, Chennai, India. prasanna_neelakantan@yahoo.com
authors.forename: P
authors.initials: P
authors.lastname: Neelakantan
authors.forename: A A
authors.initials: AA
authors.lastname: Varughese
authors.forename: S
authors.initials: S
authors.lastname: Sharma
authors.forename: C V
authors.initials: CV
authors.lastname: Subbarao
authors.forename: M
authors.initials: M
authors.lastname: Zehnder
authors.forename: G
authors.initials: G
authors.lastname: De-Deus
mesh_terms: D000704:Analysis of Variance; D002614:Chelating Agents / Q000494:pharmacology; D001840:Dental Bonding / Q000379:methods*; D003786:Dental Pulp Cavity / Q000033:anatomy & histology; D003799:Dental Stress Analysis; D003804:Dentin; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000494:pharmacology*; D004338:Drug Combinations; D004853:Epoxy Resins / Q000737:chemistry / Q000494:pharmacology*; D012968:Etidronic Acid / Q000494:pharmacology; D006801:Humans; D009532:Nickel; D012387:Root Canal Filling Materials / Q000737:chemistry* / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D018709:Statistics, Nonparametric; D014025:Titanium
chemical_list: D002614:Chelating Agents; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; C534916:epoxy resin-based root canal sealer; C040654:titanium nickelide; D009532:Nickel; D014025:Titanium; D012973:Sodium Hypochlorite; D012968:Etidronic Acid