title: Overexpression of G protein-coupled receptor 5D in the bone marrow is associated with poor prognosis in patients with multiple myeloma.
abstract: BACKGROUND
  G protein-coupled receptor 5D (GPRC5D) is a novel surface receptor. As this new subtype of G protein-coupled receptors was discovered, little is known about the role of this gene.
  
  
  METHODS
  In this retrospective study, we investigated GPRC5D mRNA expression by real-time polymerase chain reaction (RT-PCR) in bone marrow (BM) of 48 patients with multiple myeloma (MM).
  
  
  RESULTS
  Highly variable levels of GPRC5D (median, 288; quartiles, 17-928) were detected in patients with MM, whereas only low expression was detected in normal tissues (median, 1; quartiles, 1-23). High mRNA expression of GPRC5D correlated positively with high plasma cell count in bone marrow (r = 0·64, P < 0·001), high β(2) -microglobulin (r = 0·42, P = 0·003) and poor-risk cytogenetics: deletion 13q14 (rb-1), P = 0·003; and 14q32 translocation t(4;14)(p16;q32), P = 0·029. GPRC5D mRNA expression showed a significant correlation with overall survival (P = 0·031). The estimated overall survival of patients expressing GPRC5D above or below the median of 288 was 43·9% vs. 70·2% at 48 months. Here, we report, for the first time, the association of GPRC5D expression and cancer.
  
  
  CONCLUSIONS
  Overexpression in poor-risk myeloma, low expression in normal tissues and cell surface expression identify GPRC5D as a potential novel cancer antigen. Our data demonstrate that GPRC5D is a prognostic factor in MM correlating with other major risk factors.
authors.affiliation: Department of Medicine I, Division of Hematology and Hemostaseology, Medical University Vienna, Austria.
authors.forename: Johanna
authors.initials: J
authors.lastname: Atamaniuk
authors.forename: Andreas
authors.initials: A
authors.lastname: Gleiss
authors.forename: Edit
authors.initials: E
authors.lastname: Porpaczy
authors.forename: Birgit
authors.initials: B
authors.lastname: Kainz
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Grunt
authors.forename: Markus
authors.initials: M
authors.lastname: Raderer
authors.forename: Bernadette
authors.initials: B
authors.lastname: Hilgarth
authors.forename: Johannes
authors.initials: J
authors.lastname: Drach
authors.forename: Heinz
authors.initials: H
authors.lastname: Ludwig
authors.forename: Heinz
authors.initials: H
authors.lastname: Gisslinger
authors.forename: Ulrich
authors.initials: U
authors.lastname: Jaeger
authors.forename: Alexander
authors.initials: A
authors.lastname: Gaiger
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001853:Bone Marrow / Q000378:metabolism*; D003582:Cytogenetics; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics*; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D012189:Retrospective Studies; D012307:Risk Factors; D013223:Statistics as Topic; D014178:Translocation, Genetic
chemical_list: C576988:GPRC5D protein, human; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled