title: Assessment of the mutagenic, recombinagenic and carcinogenic potential of orlistat in somatic cells of Drosophila melanogaster.
abstract: In this study the mutagenic, recombinagenic, carcinogenic and anticarcinogenic potential of orlistat was assessed using the somatic mutation and recombination test (SMART) and the epithelial tumor detection test (wts). The experiments were conducted on Drosophila melanogaster. In the assessment using SMART, larvae, descendants from the standard (ST) cross and the high bioactivation (HB) cross, were treated chronically with three orlistat concentrations. The results revealed a recombinagenic effect, associated with orlistat, in the descendants of the HB cross, at all three levels of concentration. Homologous recombination can function as a determinant at different stages of carcinogenesis. For verification, larvae from the wts test, descendants of the wts/TM3 virgin female and mwh/mwh male cross, were treated with the same three orlistat concentrations separately and in association with mitomicin C (0.1mM). The results did not, however, provide evidence that orlistat has carcinogenic potential nor was it associated with the reduction of tumors induced by mitomicin C in D. melanogaster.
authors.affiliation: Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Bloco 2E, Campus Umuarama, Uberlândia, Minas Gerais, Brazil.
authors.forename: P C
authors.initials: PC
authors.lastname: Orsolin
authors.forename: R G
authors.initials: RG
authors.lastname: Silva-Oliveira
authors.forename: J C
authors.initials: JC
authors.lastname: Nepomuceno
mesh_terms: D000818:Animals; D019440:Anti-Obesity Agents / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D004331:Drosophila melanogaster; D007783:Lactones / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D000077403:Orlistat; D011995:Recombination, Genetic / Q000187:drug effects*
chemical_list: D019440:Anti-Obesity Agents; D002273:Carcinogens; D007783:Lactones; D009153:Mutagens; D000077403:Orlistat