title: Molecular and serological characterization of Leptospira interrogans serovar Canicola isolated from dogs, swine, and bovine in Brazil.
abstract: The identification of Leptospira clinical isolates through genotyping and serotyping, besides the recognition of its reservoirs, are important tools for understanding the epidemiology of leptospirosis, and they are also keys for identifying new species and serovars. Fourteen clinical isolates from animals were characterized by means of single enzyme amplified length polymorphism, variable number of tandem repeat analysis, pulsed field gel electrophoresis, and serotyping. All isolates were identified as Leptospira interrogans, serovar Canicola. Infections by this serovar occur in urban regions, where dogs represent the main maintenance hosts, whereas bovine and swine may act as reservoirs of serovar Canicola in rural areas. Both urban and rural aspects of leptospirosis, and the role of domestic animals as maintenance hosts, cannot be neglected in developing and developed countries.
authors.affiliation: Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, CEP 05508 270, São Paulo/ SP, Brazil.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Miraglia
authors.forename: Zenaide M
authors.initials: ZM
authors.lastname: de Morais
authors.forename: Odir A
authors.initials: OA
authors.lastname: Dellagostin
authors.forename: Fabiana K
authors.initials: FK
authors.lastname: Seixas
authors.forename: Julio C
authors.initials: JC
authors.lastname: Freitas
authors.forename: Francielle G S
authors.initials: FG
authors.lastname: Zacarias
authors.forename: Adina C
authors.initials: AC
authors.lastname: Delbem
authors.forename: Thaís S P
authors.initials: TS
authors.lastname: Ferreira
authors.forename: Gisele O
authors.initials: GO
authors.lastname: Souza
authors.forename: Rudy A
authors.initials: RA
authors.lastname: Hartskeerl
authors.forename: Silvio A
authors.initials: SA
authors.lastname: Vasconcellos
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Moreno
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D054458:Amplified Fragment Length Polymorphism Analysis / Q000662:veterinary; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002417:Cattle / Q000382:microbiology*; D004197:Disease Reservoirs / Q000662:veterinary*; D004285:Dogs / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005838:Genotype; D041341:Leptospira interrogans serovar canicola / Q000235:genetics*; D007922:Leptospirosis / Q000453:epidemiology* / Q000382:microbiology; D018598:Minisatellite Repeats / Q000235:genetics; D012703:Serotyping / Q000662:veterinary; D013552:Swine / Q000382:microbiology*