title: Transcriptome analysis of the duodenum, pancreas, liver, and muscle from diabetic Goto-Kakizaki rats fed a trypsin inhibitor derived from squid viscera.
abstract: Trypsin inhibitors (TIs) have various nutritional effects. However, a detailed mechanism for their effects, especially on the gene expression patterns in various tissues, remains unknown. Here, we used transcriptome techniques and gene ontology (GO) analysis to examine the effects of squid TI (sqTI), a biochemically stable peptide, on diabetic Goto-Kakizaki rats after feeding for 10 weeks. We demonstrated that downregulation of SREBP1c in the liver via duodenal/pancreatic hormones suppresses the blood cholesterol level. Consistently, in GO analysis, the term "cholesterol biosynthetic process" was enriched among downregulated genes. No hypoglycemic or insulinotropic effects were observed, in contrast to the results from our previous studies (single stimulation with the same dose of TI), which can be partly ascribed to the inactive responses of the duodenum and pancreas in this condition.
authors.affiliation: Central Research Laboratory, Nippon Suisan Kaisha, Limited, Kitanomachi, Hachioji, Tokyo, Japan. kohsukeadachi@kochi-u.ac.jp
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Adachi
authors.forename: Kana
authors.initials: K
authors.lastname: Fukumorita
authors.forename: Michihiro
authors.initials: M
authors.lastname: Araki
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Zaima
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Yang
authors.forename: Satoru
authors.initials: S
authors.lastname: Chiba
authors.forename: Hideki
authors.initials: H
authors.lastname: Kishimura
authors.forename: Hiroki
authors.initials: H
authors.lastname: Saeki
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D049832:Decapodiformes / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000008:administration & dosage*; D014781:Viscera / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; C497389:Srebf1 protein, rat; D051780:Sterol Regulatory Element Binding Protein 1; D014361:Trypsin Inhibitors