title: Native analysis of plasma-derived clotting factor VIII concentrates: "sponge effect" and contaminants.
abstract: In the present study, we analysed two commercially available plasma-derived FVIII preparations, Beriate and Emoclot, through native gel-based approaches (CN-PAGE). The rationale behind this study was to assess whether protein complexes from plasma resisted the aggressive manufacturing processes. A preliminary analysis of plasma complexes was performed focusing on the molecular weight range between 45 kDa and 1 MDa. As a result, we could evidence in both preparations the presence of complexes, which resisted the cryoprecipitation, chromatographic and heat treatment processing steps. These complexes behaved in a "sponge-effect"-like fashion through the enrichment of otherwise less abundant contaminants. The use of an alternative electrophoretic approach such as CN-PAGE allowed us to confirm 2DE-based observations and expand the list of non-FVIII proteins which contaminate the preparations.
authors.affiliation: Department of Environmental Sciences, Tuscia University, Viterbo, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Blasi
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: D'Amici
authors.forename: Angelo
authors.initials: A
authors.lastname: D'Alessandro
authors.forename: Lello
authors.initials: L
authors.lastname: Zolla
mesh_terms: D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry; D003029:Coagulants / Q000032:analysis* / Q000737:chemistry; D004340:Drug Contamination; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005169:Factor VIII / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D008970:Molecular Weight; D013678:Technology, Pharmaceutical
chemical_list: D001798:Blood Proteins; D003029:Coagulants; D005169:Factor VIII