title: [Stress resistance and genetic diversity of endophytic bacteria isolated from Caragana spp. root nodules].
abstract: By adopting PCR-RFLP and 16S rDNA sequencing, this paper analyzed the genetic diversity and phylogeny of 40 endophytic bacterial strains isolated from Caragana spp. root nodules, and determined the salt resistance, acid- and alkali resistance, and growth temperature range of the strains. A total of 9 genotypes were obtained from the 40 strains by RFLP. The 16S rDNA sequencing, morphological observation, and biochemical test of representative strains showed that the strains belonged to Bacillus, Inquilinus, Shinella and Acinetobacter, respectively, and had rich genetic diversity. 57.5% of the strains could tolerate 4% NaCl stress, 75% of the strains could grow in YMA medium with an initial pH 11.0, and 85% of the strains could survive after heat shock treatment at 60 degrees C, suggesting that the endophytic bacteria of Caragana spp. had strong resistance capacity. Among the strains, LWEN 07 and LWEN 15 were most resistant.
authors.affiliation: School of Life Science, Ningxia University, Yinchuan 750021, China. daijx05@163.com
authors.forename: Jin-Xia
authors.initials: JX
authors.lastname: Dai
authors.forename: Yu-Jiong
authors.initials: YJ
authors.lastname: Wang
authors.forename: Xue-Juan
authors.initials: XJ
authors.lastname: Wu
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Zhang
mesh_terms: D000143:Acids; D000150:Acinetobacter / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D000468:Alkalies; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D032602:Caragana / Q000382:microbiology*; D060026:Endophytes / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D014644:Genetic Variation; D010802:Phylogeny; D053204:Root Nodules, Plant / Q000382:microbiology*; D055049:Salt Tolerance / Q000502:physiology*; D012965:Sodium Chloride; D013312:Stress, Physiological
chemical_list: D000143:Acids; D000468:Alkalies; D012965:Sodium Chloride