title: Development of PCR primer sets for intron 1 of the low-copy gene LEAFY in Davalliaceae.
abstract: OBJECTIVE
  Primers were designed for amplifying intron 1 of the single-copy nuclear LEAFY gene for species of Davalliaceae.
  
  
  RESULTS
  New primer sets were designed and successfully amplified for intron 1 of the LEAFY gene in 13 species representing the five genera of Davalliaceae. The orthology of these sequences was further confirmed by phylogenetic analyses. Site variation in LEAFY intron 1 sequences across genera of the Davalliaceae and among accessions of the Humata repens complex were 18% and 8%, respectively. Such variation was greater than that for the cpDNA atpB-rbcL intergenic spacer region across the same taxa and accessions.
  
  
  CONCLUSIONS
  Using our newly designed primers, intron 1 of the LEAFY gene could be amplified for all species tested. In addition, this single-copy, biparentally inherited, and quickly evolving region showed considerable potential for addressing infraspecific-level questions.
authors.affiliation: Botanical Garden Division, Taiwan Forestry Research Institute, Taipei 100, Taiwan.
authors.forename: Cheng-Wei
authors.initials: CW
authors.lastname: Chen
authors.forename: Li-Yaung
authors.initials: LY
authors.lastname: Kuo
authors.forename: Chun-Neng
authors.initials: CN
authors.lastname: Wang
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Chiou
mesh_terms: D058893:DNA Barcoding, Taxonomic / Q000379:methods; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D029624:Ferns / Q000145:classification / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D010940:Plant Proteins