title: Northern blot analysis for expression profiling of mRNAs and small RNAs.
abstract: Northern analysis is a conventional but gold standard method for detection and quantification of gene expression changes. It not only detects the presence of a transcript but also indicates size and relative comparison of transcript abundance on a single membrane. In recent years, it has been aptly adapted to validate and study the size and expression of small noncoding RNAs. Here, we describe protocols employed in our laboratory for conventional northern analysis with total RNA/mRNA to study gene expression and validation of small noncoding RNAs using low molecular weight fraction of RNAs.
authors.affiliation: Department of Botany, University of Delhi, Delhi, India.
authors.forename: Ankur R
authors.initials: AR
authors.lastname: Bhardwaj
authors.forename: Ritu
authors.initials: R
authors.lastname: Pandey
authors.forename: Manu
authors.initials: M
authors.lastname: Agarwal
authors.forename: Surekha
authors.initials: S
authors.lastname: Katiyar-Agarwal
mesh_terms: D015152:Blotting, Northern / Q000379:methods*; D002021:Buffers; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling / Q000379:methods*; D015341:Nucleic Acid Probes / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D058727:RNA, Small Untranslated / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D002021:Buffers; D015341:Nucleic Acid Probes; D012333:RNA, Messenger; D058727:RNA, Small Untranslated