title: From the ORFeome concept to highly comprehensive, full-genome screening libraries.
abstract: Recombination-based cloning techniques have in recent times facilitated the establishment of genome-scale single-gene ORFeome repositories. Their further handling and downstream application in systematic fashion is, however, practically impeded because of logistical plus economic challenges. At this juncture, simultaneously transferring entire gene collections in compiled pool format could represent an advanced compromise between systematic ORFeome (an organism's entire set of protein-encoding open reading frames) projects and traditional random library approaches, but has not yet been considered in great detail. In our endeavor to merge the comprehensiveness of ORFeomes with a basically simple, streamlined, and easily executable single-tube design, we have here produced five different pooled screening-ready libraries for both Staphylococcus aureus and Homo sapiens. By evaluating the parallel transfer efficiencies of differentially sized genes from initial polymerase chain reaction (PCR) product amplification to entry and final destination library construction via quantitative real-time PCR, we found that the complexity of the gene population is fairly stably maintained once an entry resource has been successfully established, and that no apparent size-selection bias loss of large inserts takes place. Recombinational transfer processes are hence robust enough for straightforwardly achieving such pooled screening libraries.
authors.affiliation: Division of Molecular Dermatology, Department of Dermatology, Paracelsus Private Medical University Salzburg, Austria.
authors.forename: Raphaela
authors.initials: R
authors.lastname: Rid
authors.forename: Omar
authors.initials: O
authors.lastname: Abdel-Hadi
authors.forename: Richard
authors.initials: R
authors.lastname: Maier
authors.forename: Martin
authors.initials: M
authors.lastname: Wagner
authors.forename: Harald
authors.initials: H
authors.lastname: Hundsberger
authors.forename: Helmut
authors.initials: H
authors.lastname: Hintner
authors.forename: Johann
authors.initials: J
authors.lastname: Bauer
authors.forename: Kamil
authors.initials: K
authors.lastname: Onder
mesh_terms: D015723:Gene Library; D005820:Genetic Testing / Q000379:methods*; D015894:Genome, Human / Q000235:genetics*; D015698:Genomic Library; D006801:Humans; D013211:Staphylococcus aureus / Q000235:genetics*