title: Development of an efficient in vivo system (Pjunc-TpaseIS1223) for random transposon mutagenesis of Lactobacillus casei.
abstract: The random transposon mutagenesis system P(junc)-TpaseIS(1223) is composed of plasmids pVI129, expressing IS1223 transposase, and pVI110, a suicide transposon plasmid carrying the P(junc) sequence, the substrate of the IS1223 transposase. This system is particularly efficient in Lactobacillus casei, as more than 10,000 stable, random mutants were routinely obtained via electroporation.
authors.affiliation: UMR PAM, AgroSup Dijon et Université de Bourgogne, Dijon, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Licandro-Seraut
authors.forename: Sophie
authors.initials: S
authors.lastname: Brinster
authors.forename: Maarten
authors.initials: M
authors.lastname: van de Guchte
authors.forename: Hélène
authors.initials: H
authors.lastname: Scornec
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Maguin
authors.forename: Philippe
authors.initials: P
authors.lastname: Sansonetti
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Cavin
authors.forename: Pascale
authors.initials: P
authors.lastname: Serror
mesh_terms: D015139:Blotting, Southern; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D018274:Electroporation; D004926:Escherichia coli; D007780:Lacticaseibacillus casei / Q000235:genetics*; D016296:Mutagenesis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D019895:Transposases / Q000235:genetics
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D019895:Transposases