title: Evaluation of RapidChek Select for the screening of Salmonella in meat and meat products.
abstract: The efficacy of the RapidChek Select, an alternative rapid method based on lateral flow technology, for the screening of Salmonella in meat and meat products, was compared with the current ISO reference culture-based method. Of the 265 routine samples examined, 61 were found to be positive for Salmonella by both methods. The percentage of agreement between the results of two methods was determined as 98%. All presumptive positive results obtained by the RapidCheck Select were confirmed to be positive by ISO method. For five samples ISO method gave positive result, while RapidChek Select gave negative result. The limit of detection (LOD(50)) of RapidChek Select and ISO methods for minced beef meat samples were 1.00 cfu/25 g and 0.63 cfu/25 g, respectively. For sausage samples, LOD(50) of both methods were 2.00 cfu/25 g. As a result, the high agreement between two methods and the comparable detection limits of two methods showed that the RapidChek Select is an efficient alternative method for the screening of Salmonella in meat and meat products.
authors.affiliation: Konya University, Faculty of Science, Department of Biology, Karatay, Konya, Turkey. torlakemrah@yahoo.com
authors.forename: Emrah
authors.initials: E
authors.lastname: Torlak
authors.forename: Ibrahim Mustafa
authors.initials: IM
authors.lastname: Akan
authors.forename: Mustafa
authors.initials: M
authors.lastname: Inal
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry; D055910:Antibodies, Immobilized / Q000737:chemistry; D002417:Cattle; D005516:Food Microbiology / Q000379:methods*; D007159:Immunologic Tests / Q000592:standards; D057230:Limit of Detection; D008460:Meat / Q000382:microbiology; D011200:Poultry / Q000382:microbiology*; D012015:Reference Standards; D012475:Salmonella / Q000254:growth & development / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D055910:Antibodies, Immobilized