title: Synthesis and characterization of soybean-based hydrogels with an intrinsic activity on cell differentiation.
abstract: The successful regeneration of large defects in traumatized and diseased tissues depends on the availability of biodegradable and bioactive biomaterials able to guide the tissue during its repair by offering both a physical support and a control of its biological mechanisms. Recently, a novel class of natural, biodegradable biomaterials has been obtained by the thermosetting of defatted soy curd. These biomaterials have been shown to regulate the activity of both tissue and inflammatory cells. Here, soybean-based hydrogels with different physicochemical properties and bioactivity have been obtained with a relatively simple and highly reproducible processing method. The content of the different soy components (e.g., the isoflavones) was tuned varying the solvent system during the extraction procedure, while variations in the material crosslinking provided either loose hydrogels or a bioglue. The biomaterials obtained can be used as either bioadhesives or injectable formulations in regenerative medicine as they were shown to stimulate the synthesis of collagen by fibroblasts and the formation of mineralized bone noduli by osteoblasts.
authors.affiliation: School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, United Kingdom.
authors.forename: Steven T
authors.initials: ST
authors.lastname: Meikle
authors.forename: Guy
authors.initials: G
authors.lastname: Standen
authors.forename: Jonathan
authors.initials: J
authors.lastname: Salvage
authors.forename: Roberto
authors.initials: R
authors.lastname: De Santis
authors.forename: Luigi
authors.initials: L
authors.lastname: Nicolais
authors.forename: Luigi
authors.initials: L
authors.lastname: Ambrosio
authors.forename: Matteo
authors.initials: M
authors.lastname: Santin
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D005433:Flour; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis* / Q000494:pharmacology*; D007529:Isoflavones / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008422:Materials Testing; D012212:Rheology / Q000187:drug effects; D013025:Glycine max / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D007529:Isoflavones; D007770:L-Lactate Dehydrogenase