title: Genetic diversity analysis of  isolates of the fungal bean pathogen Pseudocercospora griseola from  central and southern Brazil.
abstract: Planting  resistant varieties is the most effective control measure against the angular  leaf spot of dry beans, a fungal disease caused by Pseudocercospora griseola.  However, dry bean varieties with durable resistance are not easily obtained.  Knowledge about the genetic variability of the pathogen population is key for  the success of dry bean breeding programs aimed at developing resistant  materials, but finding suitable operationally simple and genetically accurate  markers is not an easy task. The aim of this study was to assess the  suitability of the ISSR-PCR technique to quantify the genetic variability of P.  griseola isolates. Total DNA of 27 P. griseola isolates from Goiás,  Minas Gerais, Espírito Santo, and Paraná States was extracted and amplified  using specific primers for ISSR. Using cluster analysis, 27 genotypes were  identified. The ISSR-PCR technique was suitable for assessing intraspecific  variability of P. griseola. The ISSR-PCR marker was found to be highly  sensitive to genetic variation and can aid in elucidating the genetic structure  of the population of this plant pathogen as a support tool for the dry bean  breeding programs.
authors.affiliation: Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: A K R
authors.initials: AK
authors.lastname: Abadio
authors.forename: S S
authors.initials: SS
authors.lastname: Lima
authors.forename: M F
authors.initials: MF
authors.lastname: Santana
authors.forename: T M F
authors.initials: TM
authors.lastname: Salomão
authors.forename: A
authors.initials: A
authors.lastname: Sartorato
authors.forename: E S G
authors.initials: ES
authors.lastname: Mizubuti
authors.forename: E F
authors.initials: EF
authors.lastname: Araújo
authors.forename: M V
authors.initials: MV
authors.lastname: de Queiroz
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D001938:Brazil; D016000:Cluster Analysis; D017931:DNA Primers; D014644:Genetic Variation; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers