title: Two suppressors of RNA silencing encoded by cereal-infecting members of the family Luteoviridae.
abstract: Several members of the family Luteoviridae are important pathogens of cultivated plant species of the family Gramineae. In this study, we explored RNA-silencing suppressors (RSSs) encoded by two cereal-infecting luteoviruses: barley yellow dwarf virus and wheat yellow dwarf virus (BYDV and WYDV, respectively). The P0 protein of WYDV-GPV (P0(GPV)) and the P6 protein of BYDV-GAV (P6(GAV)) displayed RSS activities when expressed in agro-infiltrated leaves of Nicotiana benthamiana, by their local ability to inhibit post-transcriptional gene silencing of GFP. Analysis of GFP, mRNA and GFP-specific small interfering RNA indicated that both P0(GPV) and P6(GAV) are suppressors of silencing that can restrain not only local but also systemic gene silencing. This is the first report of RSS activity of the P6 protein in a member of the genus Luteovirus.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100193, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100193, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhai
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100193, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100193, PR China.
authors.forename: Beilei
authors.initials: B
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100193, PR China.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D002523:Edible Grain / Q000821:virology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D016679:Genome, Viral; D049452:Green Fluorescent Proteins; D052679:Luteoviridae / Q000502:physiology*; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified; D034622:RNA Interference / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D049452:Green Fluorescent Proteins