title: Moss-based production of asialo-erythropoietin devoid of Lewis A and other plant-typical carbohydrate determinants.
abstract: Protein therapeutics represent one of the most increasing areas in the pharmaceutical industry. Plants gain acceptance as attractive alternatives for high-quality and economical protein production. However, as the majority of biopharmaceuticals are glycoproteins, plant-specific N-glycosylation has to be taken into consideration. In Physcomitrella patens (moss), glyco-engineering is an applicable tool, and the removal of immunogenic core xylose and fucose residues was realized before. Here, we present the identification of the enzymes that are responsible for terminal glycosylation (α1,4 fucosylation and β1,3 galactosylation) on complex-type N-glycans in moss. The terminal trisaccharide consisting of α1,4 fucose and β1,3 galactose linked to N-acetylglucosamine forms the so-called Lewis A epitope. This epitope is rare on moss wild-type proteins, but was shown to be enriched on complex-type N-glycans of moss-produced recombinant human erythropoietin, while unknown from the native human protein. Via gene targeting of moss galactosyltransferase and fucosyltransferase genes, we identified the gene responsible for terminal glycosylation and were able to completely abolish the formation of Lewis A residues on the recombinant biopharmaceutical.
authors.affiliation: Plant Biotechnology, University of Freiburg, Freiburg, Germany.
authors.forename: Juliana
authors.initials: J
authors.lastname: Parsons
authors.forename: Friedrich
authors.initials: F
authors.lastname: Altmann
authors.forename: Claudia K
authors.initials: CK
authors.lastname: Arrenberg
authors.forename: Anna
authors.initials: A
authors.lastname: Koprivova
authors.forename: Anna K
authors.initials: AK
authors.lastname: Beike
authors.forename: Christian
authors.initials: C
authors.lastname: Stemmer
authors.forename: Gilbert
authors.initials: G
authors.lastname: Gorr
authors.forename: Ralf
authors.initials: R
authors.lastname: Reski
authors.forename: Eva L
authors.initials: EL
authors.lastname: Decker
mesh_terms: D000595:Amino Acid Sequence; D001212:Asialoglycoproteins / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods*; D015153:Blotting, Western; D019068:Bryopsida / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D018395:CA-19-9 Antigen; D002241:Carbohydrates / Q000737:chemistry*; D004921:Erythropoietin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism; D055786:Gene Knockout Techniques; D006020:Glycopeptides / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000378:metabolism*; D010802:Phylogeny; D011134:Polysaccharides / Q000737:chemistry; D011994:Recombinant Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001212:Asialoglycoproteins; D018395:CA-19-9 Antigen; D002241:Carbohydrates; D006020:Glycopeptides; D037102:Lectins; D009844:Oligosaccharides; D011134:Polysaccharides; D011994:Recombinant Proteins; C500605:asialoerythropoietin; D004921:Erythropoietin; D005647:Fucosyltransferases; D005700:Galactosyltransferases