title: [The involvement of actin cytoskeleton in glutoxim and molixan effect on intracellular Ca(2+)-concentration in macrophages].
abstract: Glutoxim and molixan belong to new generation of disulfide-containing drugs with immunomodulatory, hepatoprotective and hemopoetic effect on cells. Using Fura-2AM microfluorimetry, two structurally distinct actin filament disrupters, latrunculin B and cytochalasin D, and calyculin A, which causes actin filaments condensation under plasmalemma, we have shown the involvement of actin cytoskeleton in the intracellular Ca(2+)-concentration increase induced by glutoxim or molixan in rat peritoneal macrophages. Morphological data obtained with the use of rhodamine-phalloidine have demonstrated that glutoxim and molixan cause the actin cytoskeleton reorganization in rat peritoneal macrophages.
authors.forename: L S
authors.initials: LS
authors.lastname: Kurilova
authors.forename: Z I
authors.initials: ZI
authors.lastname: Krutetskaia
authors.forename: O E
authors.initials: OE
authors.lastname: Lebedev
authors.forename: N I
authors.initials: NI
authors.lastname: Krutetskaia
authors.forename: V G
authors.initials: VG
authors.lastname: Antonov
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D015638:Cytochalasin D / Q000494:pharmacology; D019610:Cytoprotection; D004338:Drug Combinations; D007155:Immunologic Factors / Q000494:pharmacology; D007288:Inosine / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D008387:Marine Toxins; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000494:pharmacology*; D010080:Oxazoles / Q000494:pharmacology; D010590:Phalloidine / Q000031:analogs & derivatives; D051381:Rats; D017208:Rats, Wistar; D012235:Rhodamines; D053778:Thiazolidines / Q000494:pharmacology
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D004338:Drug Combinations; D007155:Immunologic Factors; D008387:Marine Toxins; D009842:Oligopeptides; D010080:Oxazoles; D012235:Rhodamines; D053778:Thiazolidines; C462699:glutoxim; C000611436:inosine glycyl-cysteinyl-glutamate disodium; C504731:rhodamine-phalloidin; D010590:Phalloidine; D015638:Cytochalasin D; D007288:Inosine; C059041:calyculin A; C037068:latrunculin B; D002118:Calcium