title: Designing quantum rods for optimized energy transfer with firefly luciferase enzymes.
abstract: The bioluminescence resonance energy transfer (BRET) between firefly luciferase from Photinus pyralis (Ppy) with core/shell semiconductive quantum rods (QRs) has been studied as a function of QR aspect ratio and internal microstructure. The QRs were found to be ideal energy acceptors, and Ppy-to-core distances were optimized using rod-in-rod microstructures that were achieved by the synthetic control of rod morphology, surface chemistry, and Ppy:QR loading. The BRET ratios of >44 measured are the highest efficiencies to date.
authors.affiliation: Department of Chemistry, Syracuse University, Syracuse, New York 13244, United States.
authors.forename: Rabeka
authors.initials: R
authors.lastname: Alam
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Fontaine
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Branchini
authors.forename: Mathew M
authors.initials: MM
authors.lastname: Maye
mesh_terms: D004735:Energy Transfer; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D049409:Luciferases, Firefly / Q000737:chemistry*; D045663:Quantum Dots
chemical_list: D049409:Luciferases, Firefly