title: [Mechanisms of human plasma proteins adsorption on the surface of perfluorocarbon emulsion stabilized with proxanol 268].
abstract: It has been shown that sorption of most proteins with the molecular weight lower than 200 kDa from human blood plasma on the surface of perfluorocarbon emulsion, stabilized with proxanol 268, is mainly based on hydrophobic interaction, whereas sorption of immunoglobulin G is mainly the result of electrostatic interaction. The removal of lipidic components from plasma leads to the increase of a total amount of adsorbed proteins by 35%. Particularly, when lipidic components are removed, sorption of apolipoprotein AI and immunoglobulin G is considerably bettered as well as sorption of other proteins with the molecular weight of about 50 and 60 kDa occurs. It has been out that apolipoprotein AI in the adsorbed condition loses its capability of tryptophan fluorescence, which might be probably determined by the quenching influence of the perfluorocarbon core of nanoparticle. We think that the findings obtained also indicates considerable conformational rearrangements of this protein during adsorption. It was shown, that the fluorescence of proteins with sorption on nanoparticles in emulsion based on the hydrophobic interaction, is completely or partially quenched.
authors.forename: V K
authors.initials: VK
authors.lastname: Zhalimov
authors.forename: A N
authors.initials: AN
authors.lastname: Sklifas
authors.forename: N I
authors.initials: NI
authors.lastname: Kukushkin
mesh_terms: D000327:Adsorption; D001798:Blood Proteins / Q000737:chemistry*; D001802:Blood Substitutes / Q000737:chemistry* / Q000494:pharmacology; D004655:Emulsions / Q000737:chemistry / Q000494:pharmacology; D005466:Fluorocarbons / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D053758:Nanoparticles / Q000737:chemistry*; D011060:Poloxalene / Q000737:chemistry* / Q000494:pharmacology; D013501:Surface-Active Agents / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001798:Blood Proteins; D001802:Blood Substitutes; D004655:Emulsions; D005466:Fluorocarbons; D013501:Surface-Active Agents; D011060:Poloxalene