title: Chitosan scaffolds with BMP-6 loaded alginate microspheres for periodontal tissue engineering.
abstract: The aim of this study is to develop an effective growth factor releasing scaffold-microsphere system for promoting periodontal tissue engineering. Bone morphogenetic protein-6 (BMP-6)-loaded alginate microspheres in narrow size distribution were produced by optimising electrospraying conditions. The addition of these microspheres to chitosan gels produced a novel scaffold in which not only the pore sizes and interconnectivity were preserved, but also a controlled release vehicle was generated. Loading capacity was adjusted as 50 ng or 100 ng BMP-6 for each scaffold and the controlled release behaviour of BMP-6 from chitosan scaffolds was observed during seven days. Cell culture studies were carried out with rat mesenchymal stem cells derived from bone marrow in three groups; chitosan scaffolds, chitosan scaffolds containing BMP-6-loaded alginate microspheres and chitosan scaffolds with free BMP-6 in culture medium. Results showed that controlled delivery of BMP-6 from alginate microspheres has a significant effect on osteogenic differentiation.
authors.affiliation: Department of Bioengineering, Hacettepe University, Beytepe, Ankara, Turkey.
authors.forename: Zeliha
authors.initials: Z
authors.lastname: Soran
authors.forename: R Seda Tığlı
authors.initials: RS
authors.lastname: Aydın
authors.forename: Menemşe
authors.initials: M
authors.lastname: Gümüşderelioğlu
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D055418:Bone Morphogenetic Protein 6 / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry*; D003470:Culture Media; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010519:Periodontium / Q000378:metabolism*; D051381:Rats; D023822:Tissue Engineering
chemical_list: D000464:Alginates; D055418:Bone Morphogenetic Protein 6; D003470:Culture Media; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D048271:Chitosan