title: Lipid production of Chlorella vulgaris from lipid-extracted microalgal biomass residues through two-step enzymatic hydrolysis.
abstract: Lipid-extracted microalgal biomass residues (LMBRs) were treated using cellulase, neutrase and alcalase in a two-step process and the resulting hydrolysates were used as a source of nutrients for the cultivation of Chlorella vulgaris under non-aerated and aerated conditions for lipid production. Aeration was favorable for cell growth and lipid accumulation and a biomass of approximately 3.28 g L(-1), lipid content of 35% and lipid productivity of 116 mg L(-1) d(-1) were obtained. Thus, the tested mode of LMBRs utilization was effective for nutrient recycling in microalgal biodiesel production.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, PR China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Zheng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gao
authors.forename: Fengwei
authors.initials: F
authors.lastname: Yin
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ji
authors.forename: He
authors.initials: H
authors.lastname: Huang
mesh_terms: D000332:Aerobiosis; D000596:Amino Acids / Q000378:metabolism; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002478:Cells, Cultured; D002480:Cellulase / Q000378:metabolism; D048408:Chlorella vulgaris / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D006868:Hydrolysis; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis* / Q000302:isolation & purification*; D058086:Microalgae / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism
chemical_list: D000596:Amino Acids; D003470:Culture Media; D004798:Enzymes; D005227:Fatty Acids; D008055:Lipids; D002480:Cellulase; D013381:Subtilisins