title: First report of bovine herpesvirus 5 in bull semen.
abstract: BoHV-5 was detected in one of several extended semen samples from a healthy donor bull during routine virus screening. This was achieved by polymerase chain reaction assay (PCR) and virus isolation, with primary identification by the fluorescent antibody test. The isolated virus, B4180, was characterized by sequencing a cloned fragment of the gC gene and by restriction enzyme analysis (REA). The nucleotide sequence shared 99 % similarity with published sequences of BoHV-5, and the REA showed that the isolate was of the BoHV-5a subtype. This study provides the first evidence of intermittent BoHV-5 shedding in bull semen as well as information about its geographic distribution.
authors.affiliation: Centro Nacional de Sanidad Agropecuaria (CENSA), Autopista Nacional y Carretera de Jamaica, Apartado 10, San José de las Lajas, La Habana, Cuba.
authors.forename: Majela
authors.initials: M
authors.lastname: Rodríguez
authors.forename: Maritza
authors.initials: M
authors.lastname: Barrera
authors.forename: Oliberto
authors.initials: O
authors.lastname: Sánchez
authors.forename: Eya Caridad
authors.initials: EC
authors.lastname: Rodríguez
authors.forename: Nadia
authors.initials: N
authors.lastname: Martínez
authors.forename: Natalie C
authors.initials: NC
authors.lastname: Parra
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Toledo
mesh_terms: D000818:Animals; D002417:Cattle; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005455:Fluorescent Antibody Technique; D006566:Herpesviridae Infections / Q000662:veterinary*; D025881:Herpesvirus 5, Bovine / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012661:Semen / Q000821:virology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral