title: GABA transporter subtype 1 and GABA transporter subtype 3 modulate glutamatergic transmission via activation of presynaptic GABA(B)  receptors in the rat globus pallidus.
abstract: The intra-pallidal application of γ-aminobutyric acid (GABA) transporter subtype 1 (GAT-1) or GABA transporter subtype 3 (GAT-3) transporter blockers [1-(4,4-diphenyl-3-butenyl)-3-piperidinecarboxylic acid hydrochloride (SKF 89976A) or 1-[2-[tris(4-methoxyphenyl)methoxy]ethyl]-(S)-3-piperidinecarboxylic acid (SNAP 5114)] reduces the activity of pallidal neurons in monkey. This effect could be mediated through the activation of presynaptic GABA(B) heteroreceptors in glutamatergic terminals by GABA spillover following GABA transporter (GAT) blockade. To test this hypothesis, we applied the whole-cell recording technique to study the effects of SKF 89976A and SNAP 5114 on evoked excitatory postsynaptic currents (eEPSCs) in the presence of gabazine, a GABA(A) receptor antagonist, in rat globus pallidus slice preparations. Under the condition of postsynaptic GABA(B) receptor blockade by the intra-cellular application of N-(2,6-dimethylphenylcarbamoylmethyl)-triethylammonium bromide (OX314), bath application of SKF 89976A (10 μM) or SNAP 5114 (10 μM) decreased the amplitude of eEPSCs, without a significant effect on its holding current and whole cell input resistance. The inhibitory effect of GAT blockade on eEPSCs was blocked by (2S)-3-[[(1S)-1-(3,4-dichlorophenyl)ethyl]amino-2-hydroxypropyl](phenylmethyl)phosphinic acid, a GABA(B) receptor antagonist. The paired-pulse ratio of eEPSCs was increased, whereas the frequency, but not the amplitude, of miniature excitatory postsynaptic currents was reduced in the presence of either GAT blocker, demonstrating a presynaptic effect. These results suggest that synaptically released GABA can inhibit glutamatergic transmission through the activation of presynaptic GABA(B) heteroreceptors following GAT-1 or GAT-3 blockade. In conclusion, our findings demonstrate that presynaptic GABA(B) heteroreceptors in putative glutamatergic subthalamic afferents to the globus pallidus are sensitive to increases in extracellular GABA induced by GAT inactivation, thereby suggesting that GAT blockade represents a potential mechanism by which overactive subthalamopallidal activity may be reduced in parkinsonism.
authors.affiliation: Division of Neuroscience, Yerkes National Primate Research Center, Emory University, 954 Gatewood Road NE, Atlanta, GA 30329, USA.
authors.forename: Xiao-Tao
authors.initials: XT
authors.lastname: Jin
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Paré
authors.forename: Yoland
authors.initials: Y
authors.lastname: Smith
mesh_terms: D000818:Animals; D000840:Anisoles / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials; D050485:GABA Plasma Membrane Transport Proteins / Q000187:drug effects / Q000502:physiology*; D058805:GABA Uptake Inhibitors / Q000494:pharmacology; D058788:GABA-B Receptor Antagonists / Q000494:pharmacology; D005917:Globus Pallidus / Q000502:physiology*; D018698:Glutamic Acid / Q000502:physiology; D009557:Nipecotic Acids / Q000494:pharmacology; D010721:Phosphinic Acids / Q000494:pharmacology; D011412:Propanolamines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*
chemical_list: C092337:1-(2-(tris(4-methoxyphenyl)methoxy)ethyl)-3-piperidinecarboxylic acid; D000840:Anisoles; D050485:GABA Plasma Membrane Transport Proteins; D058805:GABA Uptake Inhibitors; D058788:GABA-B Receptor Antagonists; D009557:Nipecotic Acids; D010721:Phosphinic Acids; D011412:Propanolamines; D018080:Receptors, GABA-B; C493415:Slc6a1 protein, rat; C493417:Slc6a11 protein, rat; C085075:CGP 55845A; D018698:Glutamic Acid; C045598:N-(4,4-diphenyl-3-butenyl)nipecotic acid