title: Biofilm inhibition by an experimental dental resin composite containing octenidine dihydrochloride.
abstract: OBJECTIVE
  The aim of the present study was to investigate an antimicrobial additive containing experimental resin composite with regards to its impact on biofilm formation under oral conditions.
  
  
  METHODS
  Biofilms were established in situ on composite specimens (n=192) which contained octenidine dihydrochloride (ODH, 3 wt.% or 6 wt.%). Samples without antimicrobial additive served as control (n=96). Composite specimens were fixed on custom made splints and exposed to the oral cavity of six healthy volunteers for three or seven days. Biofilm formation was assessed by scanning electron microscopy (SEM) and fluorescence microscopy (FM).
  
  
  RESULTS
  The biofilm formation was significantly reduced on ODH containing samples compared to controls after three as well as after seven days in situ. FM evaluation additionally showed a lower viability of the reduced biofilms for both ODH concentrations.
  
  
  CONCLUSIONS
  During this short term investigation, incorporation of ODH into resin based composite materials caused biofilm inhibiting effects in situ.
authors.affiliation: Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Saarland University Hospital, Homburg/Saar, Germany. Stefan.rupf@uks.eu
authors.forename: Stefan
authors.initials: S
authors.lastname: Rupf
authors.forename: Markus
authors.initials: M
authors.lastname: Balkenhol
authors.forename: Tim O
authors.initials: TO
authors.lastname: Sahrhage
authors.forename: Alexandra
authors.initials: A
authors.lastname: Baum
authors.forename: Julia N
authors.initials: JN
authors.lastname: Chromik
authors.forename: Klaus
authors.initials: K
authors.lastname: Ruppert
authors.forename: Dirk K
authors.initials: DK
authors.lastname: Wissenbach
authors.forename: Hans H
authors.initials: HH
authors.lastname: Maurer
authors.forename: Matthias
authors.initials: M
authors.lastname: Hannig
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D000328:Adult; D000890:Anti-Infective Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D003188:Composite Resins / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007097:Imines; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D009055:Mouth / Q000382:microbiology*; D011140:Polyurethanes / Q000737:chemistry / Q000494:pharmacology*; D011725:Pyridines / Q000737:chemistry / Q000494:pharmacology*; D012044:Regression Analysis; D018709:Statistics, Nonparametric
chemical_list: D000180:Acrylic Resins; D000890:Anti-Infective Agents; C029012:Composite Dental Resin; D003188:Composite Resins; D007097:Imines; D011140:Polyurethanes; D011725:Pyridines; C034213:octenidine