title: Synthesis of adenosine functionalized metal immobilized magnetic nanoparticles for highly selective and sensitive enrichment of phosphopeptides.
abstract: A new type of IMAC material, with ATP as the chelating ligand, was synthesized and applied to capture phosphopeptides. For the first time, the approach for phosphopeptide enrichment could provide selectivity under 5000-fold dilution by nonphosphopeptides, and sensitivity of on-target enrichment at 3 amol.
authors.affiliation: National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Dalian 116023, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhang
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liang
authors.forename: Kaiguang
authors.initials: K
authors.lastname: Yang
authors.forename: Liangliang
authors.initials: L
authors.lastname: Sun
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000241:Adenosine / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D010316:Particle Size; D010748:Phosphopeptides / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D058185:Magnetite Nanoparticles; D009942:Organometallic Compounds; D010748:Phosphopeptides; D000241:Adenosine