title: Splenectomy delays uterine natural killer cell recruitment to implantation sites and prolongs pregnancy in mice.
abstract: Uterine natural killer (uNK) cells are the dominant lymphocytes found in pregnant mammals that develop uterine decidualization. Four stages of mouse uNK cell differentiation are recognized using Dolichos biflorus agglutinin (DBA) lectin histochemistry. Each uNK cell subtype has a preferential domain. In human and mouse normal pregnancies, uNK cells are activated interferon gamma-producing cells that promote angiogenesis and development of the decidua and placenta. Murine transplant models suggest that uNK cells differentiate from self-renewing progenitors found in peripheral secondary lymphoid tissues, particularly spleen and lymph nodes. In this work, the spleen was removed 7 days before mice were mated to address whether absence of the spleen reduced uNK cell numbers or altered the distributions of maturing uNK cell subsets using quantitative lectin histochemistry. Splenectomy delayed uNK cell maturation within implantation sites. This coincided with delayed decidual and placental development and a significant (48 hr) lengthening of gestation without loss of viability. These studies characterize spleen as a biologically important progenitor tissue for uNK precursor cells.
authors.affiliation: Integrative Animal Biology Laboratory, Biomedical Science Institute, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Zavan
authors.forename: Celiane Cardoso
authors.initials: CC
authors.lastname: Carvalho
authors.forename: Wagner Costa
authors.initials: WC
authors.lastname: Rossi Junior
authors.forename: Andréa Mollica Amarante
authors.initials: AM
authors.lastname: Paffaro
authors.forename: Valdemar Antonio
authors.initials: VA
authors.lastname: Paffaro Junior
mesh_terms: D000818:Animals; D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000502:physiology*; D008297:Male; D051379:Mice; D037121:Plant Lectins; D011247:Pregnancy; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013156:Splenectomy; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014599:Uterus / Q000166:cytology / Q000502:physiology*
chemical_list: D037121:Plant Lectins