title: [Simulation of the uncoupling activity of fatty acids with the participation of ADP/ATP and aspartate/glutamate antiporters in liver mitochondria].
abstract: It has been found that the protonophoric specific uncoupling activity of palmitic acid in rat liver mitochondria does not change as its concentration increases from 5 to 40 microM. Under these conditions, the component of the specific uncoupling activity, which describes the participation in uncoupling of the ADP/ATP antiporter (sensitive to carboxyatractylate), increases, and the component of specific uncoupling activity, which characterizes the participation in the uncoupling of the aspartate/glutamate antiporter (sensitive to glutamate), decreases by the same value. A kinetic model of the fatty acid-induced uncoupling activity with the participation of ADP/ATP and aspartate/glutamate antiporters has been developed. According to the model, these carriers can exist in two forms: an active, i.e., participating in the uncoupling, and an inactive. The interaction of a fatty acid with the regulator site of the ADP/ATP antiporter translates it from the inactive to the active form, while the interaction of a fatty acid with the regulator site of the aspartate/glutamate antiporter, on the contrary, translates it from the active form to inactive. The velocity of transport of a fatty acid anion by the antiporter from the internal monolayer of the internal membrane to the external monolayer is proportional to the product of the concentration of the fatty acid and the active form of this carrier. A good conformity of the model to experimentally obtained data is shown provided that (a) ADP/ATP and aspartate/glutamate antiporters, being completely in an active state, transfer fatty acid anions with the same velocity; (b) the equilibrium dissociation constants of a complex of the carrier with the fatty acid in these antiporters are equal.
authors.forename: V N
authors.initials: VN
authors.lastname: Samartsev
authors.forename: O V
authors.initials: OV
authors.lastname: Kozhina
authors.forename: E I
authors.initials: EI
authors.lastname: Marchik
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D026922:Amino Acid Transport Systems, Acidic / Q000378:metabolism*; D000818:Animals; D017920:Antiporters / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism; D001693:Biological Transport, Active / Q000502:physiology; D005227:Fatty Acids / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D008954:Models, Biological; D051381:Rats
chemical_list: D026922:Amino Acid Transport Systems, Acidic; D017920:Antiporters; D005227:Fatty Acids; D024101:Mitochondrial Proteins; D001224:Aspartic Acid; D018698:Glutamic Acid; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate