title: Efficient introduction of specific TP53 mutations into mouse embryonic fibroblasts and embryonic stem cells.
abstract: This protocol describes a rapid, precise method for generating sets of embryonic stem (ES) cells or mouse embryonic fibroblasts (MEFs) harboring point mutations in the p53 tumor suppressor gene (officially known as Trp53). The strategy uses cells from the Trp53 (p53-null) 'platform' mouse, which allows site-specific integration of plasmid DNA into the Trp53 locus. Simple PCR protocols identify correctly targeted clones and immunoblots verify re-expression of the protein. We also present protocol modifications needed for efficient recovery of MEF clones expressing p53 constructs that retain wild-type function, including growth at low (3%) oxygen and transient downregulation of p53 regulators to forestall cell senescence of primary MEFs. A library of cell lines expressing various p53 mutants derived from the same population of primary fibroblasts or platform ES cells can be acquired and screened in less than 1 month.
authors.affiliation: Genetic Alterations in Carcinogenesis (C016), German Cancer Research Center (Deutsches Krebsforschungszentrum), Heidelberg, Germany.
authors.forename: Quan-Xiang
authors.initials: QX
authors.lastname: Wei
authors.forename: Franciscus
authors.initials: F
authors.lastname: van der Hoeven
authors.forename: Monica
authors.initials: M
authors.lastname: Hollstein
authors.forename: Adam F
authors.initials: AF
authors.lastname: Odell
mesh_terms: D000818:Animals; D001483:Base Sequence; D053686:Fetal Stem Cells; D005347:Fibroblasts; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53