title: Amphiphilic cholic-acid-modified dextran sulfate and its application for the controlled delivery of superoxide dismutase.
abstract: A novel amphiphilic and biodegradable polyelectrolyte DS-CA is prepared by the esterification of DS with CA. DS-CA can self-assemble into stable nanoparticles in water. SOD can effectively associate with DS-CA at pH = 5.0 by virtue of electrostatic and hydrophobic interactions. SOD release from the complex nanoparticles is slow at pH = 1.2. The release at pH = 7.4 PBS shows an extended behavior and is tunable by changing the weight ratio of SOD to DS-CA as well as the CA substitution degree. Increasing the CA substitution degree of DS-CA can significantly enhance the cellular uptake of the loaded SOD. This study demonstrates that the amphiphilic DS-CA provides a promising strategy for oral delivery of protein/peptide drugs.
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
authors.forename: Yubing
authors.initials: Y
authors.lastname: Xiong
authors.forename: Jianing
authors.initials: J
authors.lastname: Qi
authors.forename: Ping
authors.initials: P
authors.lastname: Yao
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D019826:Cholic Acid / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000138:chemical synthesis* / Q000494:pharmacology; D016264:Dextran Sulfate / Q000737:chemistry*; D004705:Endocytosis / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D010316:Particle Size; D055672:Static Electricity; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000494:pharmacology; D013501:Surface-Active Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D014867:Water
chemical_list: D003692:Delayed-Action Preparations; D013501:Surface-Active Agents; D014867:Water; D016264:Dextran Sulfate; D013482:Superoxide Dismutase; D019826:Cholic Acid