title: Supercooling and vitrification of aqueous glycerol solutions at normal and high pressures.
abstract: The supercooling and vitrification of aqueous glycerol solutions was studied at high pressures. Homogeneous ice nucleation temperatures (T(H)) were obtained for aqueous glycerol solutions of R=50, 30, 20, 12, and 10 (R: moles of water/moles of glycerol) up to 300MPa. The R=20 glycerol solution formed a glass above 200MPa at a cooling rate of 200°C/min, indicating that pressure enhances glass-formation of aqueous glycerol solutions. The (dT(g)/dP) values were obtained for vitrified aqueous glycerol solutions of R=3, 5, 10, and 20. These data can be used for the development of cryo-preservation liquids for living cells at high pressures.
authors.affiliation: Department of Applied Chemistry, National Defense Academy, Yokosuka 239-8686, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Miyata
authors.forename: S
authors.initials: S
authors.lastname: Hayakawa
authors.forename: K
authors.initials: K
authors.lastname: Kajiwara
authors.forename: H
authors.initials: H
authors.lastname: Kanno
mesh_terms: D003080:Cold Temperature; D003451:Cryoprotective Agents / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D011312:Pressure; D044366:Transition Temperature; D058989:Vitrification; D014867:Water / Q000737:chemistry
chemical_list: D003451:Cryoprotective Agents; D014867:Water; D005990:Glycerol