title: Transferring chromosome DNA fragments from multiple donor cells into a host strain for yeast strain improvement.
abstract: Based on a common biological phenomenon - homologous recombination - a novel method was developed by transferring chromosome DNA fragments extracted from multiple donor cells into a host strain. Through this method of transferring DNA fragments, foreign DNA fragments are introduced into one host cell and multiple positive traits from multiple strains may be integrated into the host strain. We first confirmed its feasibility in both prokaryotic and eukaryotic cells by selecting reverse mutants to prototrophy from auxotrophic strains through receiving chromosomal DNA fragments of wild-type parental strains. We then applied this method to Saccharomyces cerevisiae to improve its ethanol and temperature tolerance. We introduced donor chromosome DNA fragments from different S. cerevisiae strains with improvements in ethanol or temperature tolerance into a common strain S. cerevisiae and obtained a strain with much superior ethanol and temperature tolerance. The results showed that the Transferring DNA Fragments method provides a new way for strain breeding.
authors.affiliation: Department of Bioengineering, College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, People's Republic of China.
authors.forename: Xian'ai
authors.initials: X
authors.lastname: Shi
authors.forename: Hang
authors.initials: H
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Wang
authors.forename: Chun
authors.initials: C
authors.lastname: Meng
mesh_terms: D004247:DNA / Q000235:genetics; D018014:Gene Transfer Techniques; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D004247:DNA