title: Prevalence of JC virus in Chinese patients with colorectal cancer.
abstract: BACKGROUND
  JCV is a DNA polyomavirus very well adapted to humans. Although JCV DNA has been detected in colorectal cancers (CRC), the association between JCV and CRC remains controversial. In China, the presence of JCV infection in CRC patients has not been reported. Here, we investigated JCV infection and viral DNA load in Chinese CRC patients and to determine whether the JCV DNA in peripheral blood (PB) can be used as a diagnostic marker for JCV-related CRC.
  
  
  RESULTS
  Tumor tissues, non-cancerous tumor-adjacent tissues and PB samples were collected from 137 CRC patients. In addition, 80 normal colorectal tissue samples from patients without CRC and PB samples from 100 healthy volunteers were also harvested as controls. JCV DNA was detected by nested PCR and glass slide-based dot blotting. Viral DNA load of positive samples were determined by quantitative real-time PCR. JCV DNA was detected in 40.9% (56/137) of CRC tissues at a viral load of 49.1 to 10.3×10(4) copies/µg DNA. Thirty-four (24.5%) non-cancerous colorectal tissues (192.9 to 4.4×10(3) copies/µg DNA) and 25 (18.2%) PB samples (81.3 to 4.9×10(3) copies/µg DNA) from CRC patients were positive for JCV. Tumor tissues had higher levels of JCV than non-cancerous tissues (P = 0.003) or PB samples (P<0.001). No correlation between the presence of JCV and demographic or medical characteristics was observed. The JCV prevalence in PB samples was significantly associated with the JCV status in tissue samples (P<0.001). Eleven (13.8%) normal colorectal tissues and seven (7.0%) PB samples from healthy donors were positive for JCV.
  
  
  CONCLUSIONS
  JCV infection is frequently present in colorectal tumor tissues of CRC patients. Although the association between JCV presence in PB samples and JCV status in tissue samples was identified in this study, whether PB JCV detection can serve as a marker for JCV status of CRC requires further study.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Mou
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Fanlong
authors.initials: F
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.forename: Pingping
authors.initials: P
authors.lastname: Diao
authors.forename: Haohao
authors.initials: H
authors.lastname: Wang
authors.forename: Yifei
authors.initials: Y
authors.lastname: Li
authors.forename: Jianjiang
authors.initials: J
authors.lastname: Lin
authors.forename: Lisong
authors.initials: L
authors.lastname: Teng
authors.forename: Charlie
authors.initials: C
authors.lastname: Xiang
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People; D001483:Base Sequence; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D015179:Colorectal Neoplasms / Q000150:complications / Q000453:epidemiology / Q000821:virology*; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D027601:Polyomavirus Infections / Q000150:complications / Q000453:epidemiology / Q000821:virology*; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000150:complications / Q000453:epidemiology / Q000821:virology*; D019562:Viral Load
chemical_list: D017931:DNA Primers; D004279:DNA, Viral