title: Complete nucleotide sequence of a new strain of grapevine leafroll-associated virus 3 in South Africa.
abstract: The complete genome sequences of two newly identified genetic variants of grapevine leafroll-associated virus 3 (GLRaV-3) are described. Two isolates, GH11 (18671 nt) and GH30 (18576 nt) were sequenced and found to be less than 70 % similar to other South African GLRaV-3 variants. The genome organization of GH11 and GH30 is similar to that of previously described GLRaV-3 isolates. However, no corresponding open reading frame 2 (ORF2) could be identified. Phylogenetic analysis indicates that GH11 and GH30 cluster in a subgroup (group VI) that is separate from the previously described GLRaV-3 isolates and should be regarded as a different strain of GLRaV-3.
authors.affiliation: Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch 7602, South Africa.
authors.forename: R
authors.initials: R
authors.lastname: Bester
authors.forename: H J
authors.initials: HJ
authors.lastname: Maree
authors.forename: J T
authors.initials: JT
authors.lastname: Burger
mesh_terms: D029381:Closteroviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016000:Cluster Analysis; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013019:South Africa; D026801:Synteny; D027843:Vitis / Q000821:virology*
chemical_list: D012367:RNA, Viral