title: Outbreak of mastitis in sheep caused by multi-drug resistant Enterococcus faecalis in Sardinia, Italy.
abstract: An outbreak of infective mastitis due to Enterococcus faecalis occurred in an intensive sheep farm in north Sardinia (Italy). E. faecalis, which is only rarely isolated from sheep milk, was unexpectedly found in 22·3% of positive samples at microbiological examination. Forty-five out of the 48 E. faecalis isolates showed the same multi-drug resistance pattern (cloxacillin, streptomycin, kanamycin, clindamycin, oxytetracycline). E. faecalis isolates were analysed by pulsed-field gel electrophoresis, and all 45 multi-drug resistant strains showed an indistinguishable macrorestiction profile, indicating their clonal origin. To our knowledge, this is the first report of an outbreak of mastitis in sheep caused by E. faecalis.
authors.affiliation: Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Sanciu
authors.affiliation: National Reference Centre for Sheep and Goat Mastitis, Istituto Zooprofilattico Sperimentale della Sardegna G. Pegreffi, Sassari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Marogna
authors.affiliation: Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Paglietti
authors.affiliation: Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Cappuccinelli
authors.affiliation: National Reference Centre for Sheep and Goat Mastitis, Istituto Zooprofilattico Sperimentale della Sardegna G. Pegreffi, Sassari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Leori
authors.affiliation: Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Rappelli
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002981:Clindamycin / Q000494:pharmacology; D003023:Cloxacillin / Q000494:pharmacology; D003612:Dairying; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial; D013293:Enterococcus faecalis / Q000187:drug effects / Q000302:isolation & purification*; D005260:Female; D007558:Italy / Q000453:epidemiology; D007612:Kanamycin / Q000494:pharmacology; D008413:Mastitis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008892:Milk / Q000382:microbiology*; D010118:Oxytetracycline; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000382:microbiology*; D013307:Streptomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D002981:Clindamycin; D007612:Kanamycin; D003023:Cloxacillin; D010118:Oxytetracycline; D013307:Streptomycin