title: High levels of gene expression in the hepatocytes of adult mice, neonatal mice and tree shrews via retro-orbital sinus hydrodynamic injections of naked plasmid DNA.
abstract: Hydrodynamic-based gene delivery has emerged as an efficient and simple method for the intracellular transfection of naked plasmid DNA (pDNA) in vivo. In this system, a hydrodynamic injection via the tail vein is the most effective non-viral method of liver-targeted gene delivery. However, this injection is often technically challenging when used in animals whose tail veins are difficult to visualize or too small to operate on. To overcome this limitation, an alternative in vivo gene delivery method, the rapid injection of large volume of pDNA solution through retro-orbital sinus, was established. Using this technique, we successfully delivered pDNA to the tissue of adult mice, neonatal mice and tree shrews. The efficient expression of exogenous genes was specifically detected in the liver of test animals treated with this gene delivery method. This study demonstrates for the first time that the hydrodynamic gene delivery via the retro-orbital sinus can not only reach the same transgene efficiency as a tradition hydrodynamic-based intravascular injection but also be used in animals that are difficult to inject via the tail vein. This method could open up new areas in gene function studies and gene therapy disease treatment.
authors.affiliation: Laboratory of Blood-borne Virus, Beijing Institute of Transfusion Medicine, 27(9) Tai Ping Road, Beijing, China.
authors.forename: Shaoduo
authors.initials: S
authors.lastname: Yan
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Fu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jidong
authors.initials: J
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiangguo
authors.initials: X
authors.lastname: Duan
authors.forename: Shuaizheng
authors.initials: S
authors.lastname: Jia
authors.forename: Jianchun
authors.initials: J
authors.lastname: Peng
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhou
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.forename: Linsheng
authors.initials: L
authors.lastname: Zhan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004247:DNA / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005123:Eye; D015870:Gene Expression; D018014:Gene Transfer Techniques; D022781:Hepatocytes / Q000378:metabolism*; D007267:Injections; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D049409:Luciferases, Firefly / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D012788:Shrews; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004247:DNA; D049409:Luciferases, Firefly; D001616:beta-Galactosidase