title: Simultaneous detection of gastric acid and histamine release to unravel the regulation of acid secretion from the guinea pig stomach.
abstract: Gastric acid secretion is regulated by three primary components that activate the parietal cell: histamine, gastrin, and acetylcholine (ACh). Although much is known about these regulatory components individually, little is known on the interplay of these multiple activators and the degree of regulation they pose on the gastric acid secretion mechanism. We utilized a novel dual-sensing approach, where an iridium oxide sensor was used to monitor pH and a boron-doped diamond electrode was used for the detection of histamine from in vitro guinea pig stomach mucosal sections. Under basal conditions, gastrin was shown to be the main regulatory component of the total acid secretion and directly activated the parietal cell rather than by mediating gastric acid secretion through the release of histamine from the enterochromaffin-like cell, although both pathways were active. Under stimulated conditions with ACh, the gastrin and histamine components of the total acid secretion were not altered compared with levels observed under basal conditions, suggestive that ACh had no direct effect on the enterochromaffin-like cell and G cell. These data identify a new unique approach to investigate the regulation pathways active during acid secretion and the degree that they are utilized to drive total gastric acid secretion. The findings of this study will enhance our understanding on how these signaling mechanisms vary under pathophysiology or therapeutic management.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, United Kingdom.
authors.forename: Eleni
authors.initials: E
authors.lastname: Bitziou
authors.forename: Bhavik Anil
authors.initials: BA
authors.lastname: Patel
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D019861:Enterochromaffin-like Cells / Q000378:metabolism; D005744:Gastric Acid / Q000378:metabolism*; D019863:Gastrin-Secreting Cells / Q000378:metabolism; D005755:Gastrins; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000378:metabolism*; D013270:Stomach / Q000187:drug effects / Q000502:physiology*
chemical_list: D005755:Gastrins; D006632:Histamine; D000109:Acetylcholine