title: Production of truncated protein by the crosslink formation of mRNA with 2'-OMe oligoribonucleotide containing 2-amino-6-vinylpurine.
abstract: The development of convenient methods for controlling the protein expression is an important challenge in the postgenomic era. We applied the crosslink forming oligonucleotide (CFO) as a terminator of the ribosomal translation. In this study, we demonstrated that the improved reactivity of our CFO under physiological conditions enabled the sequence-specific introduction of a steric block for a ribosome on mRNAs. In vitro and in cell translation experiments revealed that the crosslinked mRNA can produce the truncated proteins in which the translation terminates at the desired position.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Miyagi 980-8577, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Hagihara
authors.forename: Shuhei
authors.initials: S
authors.lastname: Kusano
authors.forename: Wei-Chen
authors.initials: WC
authors.lastname: Lin
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Chao
authors.forename: Tsuneaki
authors.initials: T
authors.lastname: Hori
authors.forename: Shuhei
authors.initials: S
authors.lastname: Imoto
authors.forename: Fumi
authors.initials: F
authors.lastname: Nagatsugi
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000235:genetics* / Q000378:metabolism; D010443:Peptide Chain Termination, Translational / Q000235:genetics*; D014176:Protein Biosynthesis; D015202:Protein Engineering; D011687:Purines / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D014753:Vinyl Compounds / Q000737:chemistry / Q000378:metabolism*
chemical_list: C432203:2-amino-6-vinylpurine; D009843:Oligoribonucleotides; D011687:Purines; D012333:RNA, Messenger; D014753:Vinyl Compounds