title: RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma.
abstract: A simple, accurate, and precise HPLC method was developed and validated for determination of rimonabant in human plasma. Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile : water (90 : 10, v/v), drug was detected at 260 nm using UVdetector. The LOD and LOQ were 3.0 and 10.0 μg/L, respectively. The method is linear in the interval 50.0-1000.0 μg/L. The average extraction recovery of drug from plasma was found to be 92.2%. The percent CV of the method was found to be less than 10.8%, and accuracy was found between 94.5 and 106.7%. The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant.
authors.affiliation: Department of Pharmaceutical Chemistry, Rajiv Gandhi Prodhyugiki Mahavidhyalaya, Bhopal 462036, India.
authors.forename: Shravan
authors.initials: S
authors.lastname: Bankey
authors.forename: Ganesh
authors.initials: G
authors.lastname: Tapadiya
authors.forename: Jasvant
authors.initials: J
authors.lastname: Lamale
authors.forename: Deepti
authors.initials: D
authors.lastname: Jain
authors.forename: Shweta
authors.initials: S
authors.lastname: Saboo
authors.forename: S S
authors.initials: SS
authors.lastname: Khadabadi