title: Characterization of the natural variation in  Arabidopsis thaliana metabolome by the analysis of metabolic distance.
abstract: Metabolite fingerprinting is widely used to unravel the chemical characteristics of biological samples. Multivariate data analysis and other statistical tools are subsequently used to analyze and visualize the plasticity of the metabolome and/or the relationship between those samples. However, there are limitations to these approaches for example because of the multi-dimensionality of the data that makes interpretation of the data obtained from untargeted analysis almost impossible for an average human being. These limitations make the biological information that is of prime importance in untargeted studies be partially exploited. Even in the case of full exploitation, current methods for relationship elucidation focus mainly on between groups variation and differences. Therefore, a measure that is capable of exploiting both between- and within-group biological variation would be of great value. Here, we examined the natural variation in the metabolome of nine Arabidopsis thaliana accessions grown under various environmental conditions and established a measure for the metabolic distance between accessions and across environments. This data analysis approach shows that there is just a minor correlation between genetic and metabolic diversity of the nine accessions. On the other hand, it delivers so far in Arabidopsis unexplored chemical information and is shown to be biologically relevant for resistance studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0375-3) contains supplementary material, which is available to authorized users.
authors.forename: Benyamin
authors.initials: B
authors.lastname: Houshyani
authors.forename: Patrick
authors.initials: P
authors.lastname: Kabouw
authors.forename: Dorota
authors.initials: D
authors.lastname: Muth
authors.forename: Ric C H
authors.initials: RC
authors.lastname: de Vos
authors.forename: Raoul J
authors.initials: RJ
authors.lastname: Bino
authors.forename: Harro J
authors.initials: HJ
authors.lastname: Bouwmeester