title: Using a combination of computational and experimental techniques to understand the molecular basis for protein allostery.
abstract: Allostery is the process by which remote sites of a system are energetically coupled to elicit a functional response. The early models of allostery such as the Monod-Wyman-Changeux model and the Koshland-Némethy-Filmer model explain the allosteric behavior of multimeric proteins. However, these models do not explain how allostery arises from atomic level in detail. Recent developments in computational methods and experimental techniques have led the beginning of a new age in studying allostery. The combination of computational methods and experiments is a powerful research approach to help answering questions regarding allosteric mechanism at atomic resolution. In this review, three case studies are discussed to illustrate how this combined approach helps to increase our understanding of protein allostery.
authors.affiliation: Biomolecular Interaction Centre, Department of Chemistry, University of Canterbury, Christchurch, New Zealand.
authors.forename: Wanting
authors.initials: W
authors.lastname: Jiao
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Parker
mesh_terms: D000494:Allosteric Regulation; D000495:Allosteric Site; D000591:Amino Acid Isomerases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D019295:Computational Biology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D005941:Glucokinase / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D011487:Protein Conformation; D015398:Signal Transduction
chemical_list: D005941:Glucokinase; D017868:Cyclic AMP-Dependent Protein Kinases; D000591:Amino Acid Isomerases; C062292:glutamate racemase