title: [Progress with research on the permeability characteristics of reproductive cell membranes].
abstract: The successful cryopreservation of reproductive cells has important practical significance in many fields. In order to improve the recovery rate and viability of cryopreserved cells, it is necessary to study the permeability characteristics of cell membrane to both water and cryoprotectant. In this paper we review the studies on membrane permeability of animal reproductive cell for the recent years. We firstly list the typical permeability data of spermatozoa and oocyte membrane for water and cryoprotectant. We then analyze the effects of these characteristics on the design of cryopreservation protocol. We also introduce the latest experimental methods to measure the cell membrane permeability.
authors.affiliation: Institute of Refrigeration and Cryogenics, Zhejiang University, Hangzhou 310027, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.forename: Guangming
authors.initials: G
authors.lastname: Chen
authors.forename: Shaozhi
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D009865:Oocytes / Q000166:cytology*; D013094:Spermatozoa / Q000166:cytology*