title: Simple, cheap and effective high-performance liquid chromatographic method for determination of praziquantel in bovine muscle.
abstract: A simple high-performance liquid chromatographic method using ultraviolet detection was developed for the determination of praziquantel in bovine muscle. The sample was extracted with ethyl acetate, cleaned up by alumin B cartridge. Analyses were run at a flow-rate of 0.8 ml/min with the detector operating at a detection wavelength of 220 nm. The method is specific and sensitive, with a quantification limit of 0.02 mg/kg and a detection limit of 0.01 mg/kg. The standard calibration curve of drugs solution was linear in the range of 0.02-2.0 μg/ml (r² = 0.9999). At the fortified levels of 0.02, 0.05, 0.2 mg/kg, the mean recoveries of praziquantel ranged from 75% to 85%, while the intra-day and inter-day coefficient of variation (CV) of the assay were all less than 9%.
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.forename: Shi-Jin
authors.initials: SJ
authors.lastname: Bu
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry*; D011223:Praziquantel / Q000032:analysis*
chemical_list: D000871:Anthelmintics; D011223:Praziquantel