title: Rapid screening and cultivation of oleaginous microorganisms.
abstract: Oleaginous microbial strains were cultivated to identify the best oil-producing strain amongst Yarrowia lipolytica (CGMCC 2.1398), Lipomyces starkeyi (CGMCC 2.1608), Rhodosporidium toruloides (CGMCC 2.1389), Mortierella isabellina (CGMCC 3.3410), Cunninghamella blakeleana (CGMCC 3.970), and Mycobacterium QJ311. A method for rapid determination of oil content and fatty acid composition was established to identify the optimum oil-producing strains. This method had a relative standard deviation of 4.09%, an average recovery ratio of 97.09% and a detection limit of 0.1-1.0 g. Mortierella isabellina CGMCC 3.3410 was identified as the best oil-producing strain amongst the six strains tested, with a total biomass of 75 g/10 L and a lipid content of 35%. A rapid screening method of oleaginous microorganisms is discussed for the first time.
authors.affiliation: School of Chemical Engineering and Pharmacy, Hubei Key Lab of Novel Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, China.
authors.forename: Xinlei
authors.initials: X
authors.lastname: Gao
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhongju
authors.initials: Z
authors.lastname: Che
authors.forename: Li
authors.initials: L
authors.lastname: Wu
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D002849:Chromatography, Gas; D004951:Esterification; D008855:Microscopy, Electron, Scanning