title: Evaluation of effectiveness of EDTA and sodium thiosulfate in removing metal toxicity toward sea urchin embryo-larval applying the TIE.
abstract: Since the development of the TIE (Toxicity Identification and Evaluation) in 1988 it has been assumed that the capacity of EDTA and sodium thiosulfate to complex some metals, and thus remove their toxicity, can be applied to both freshwater and seawater ecotoxicological tests and the results subsequently interpreted. However, it is now known that there is a wide variability in the extent of this complexation. In this context, the removal of toxicity caused by the presence of Hg(2+), Cd(2+), Cu(2+), Cr(6+), Zn(2+), Ni(2+), Pb(2+), Ag(1+) and Se(2+), through metal complexation by EDTA and sodium thiosulfate, in relation to the performance of embryo-larval tests with the sea urchin Arbacia lixula was investigated. It was observed that EDTA was capable of removing the toxicity of Pb(2+), Zn(2+) and Cu(2+) while sodium thiosulfate only reduced the toxicity of Ag(1+). Compared to the complexation observed in freshwater ecotoxicological tests, the complexing agents used in this study (EDTA and sodium thiosulfate) have a lower capacity to complex metals in the marine ecotoxicological test with A. lixula.
authors.affiliation: Universidade do Vale do Itajaí - UNIVALI, Centro de Ciências Tecnológicas da Terra e do Mar - CTTMar, Cx.P. 360, Itajaí, SC 88302-202, Brazil. cresgalla@univali.br
authors.forename: C
authors.initials: C
authors.lastname: Resgalla
authors.forename: F
authors.initials: F
authors.lastname: Poleza
authors.forename: R C
authors.initials: RC
authors.lastname: Souza
authors.forename: M V
authors.initials: MV
authors.lastname: Máximo
authors.forename: C M
authors.initials: CM
authors.lastname: Radetski
mesh_terms: D000818:Animals; D056831:Coordination Complexes / Q000737:chemistry / Q000633:toxicity; D004492:Edetic Acid / Q000737:chemistry*; D007814:Larva / Q000187:drug effects; D019216:Metals, Heavy / Q000737:chemistry / Q000633:toxicity*; D012617:Sea Urchins / Q000187:drug effects / Q000254:growth & development; D013885:Thiosulfates / Q000737:chemistry*; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D056831:Coordination Complexes; D019216:Metals, Heavy; D013885:Thiosulfates; D014874:Water Pollutants, Chemical; D004492:Edetic Acid; C017717:sodium thiosulfate