title: A brief review of other notable protein detection methods on acrylamide gels.
abstract: Several methods have been described to stain proteins analyzed on acrylamide gels. These include ultrasensitive protein detection in one-dimensional and two-dimensional gel electrophoresis using a fluorescent product from the fungus Epicoccum nigrum; a fluorescence-based Coomassie Blue protein staining; visualization of proteins in acrylamide gels using ultraviolet illumination; fluorescence visualization of proteins in sodium dodecyl sulfate-polyacrylamide gels using environmentally benign, nonfixative, saline solution; and increasing the sensitivity four- to sixfold for detecting trace proteins in dye or silver stained polyacrylamide gels using polyethylene glycol 6000. All these methods are reviewed briefly in this chapter.
authors.affiliation: Department of Veterans Affairs, University of Oklahoma Health Sciences Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA. biji-kurien@omrf.org
authors.forename: Biji T
authors.initials: BT
authors.lastname: Kurien
authors.forename: R Hal
authors.initials: RH
authors.lastname: Scofield
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D002021:Buffers; D004396:Coloring Agents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry; D055772:Green Chemistry Technology / Q000379:methods*; D057230:Limit of Detection; D009942:Organometallic Compounds / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012394:Rosaniline Dyes / Q000737:chemistry; D012965:Sodium Chloride; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000180:Acrylic Resins; D002021:Buffers; D004396:Coloring Agents; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D011506:Proteins; D012394:Rosaniline Dyes; C515601:Sypro Ruby; C016680:polyacrylamide gels; D012965:Sodium Chloride; C048139:Coomassie blue