title: Regulation of Proinflammatory Mediators via NF-κB and p38 MAPK-Dependent Mechanisms in RAW 264.7 Macrophages by Polyphenol Components Isolated from Korea Lonicera japonica THUNB.
abstract: Lonicera japonica THUNB., which abundantly contains polyphenols, has been used as a traditional medicine for thousands of years in East Asian countries because of the anti-inflammation properties. This study aimed to investigate the anti-inflammatory mechanism of polyphenol components isolated from Korea L. japonica T. by nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinases (MAPKs) pathway. Polyphenols significantly decreased lipopolysaccharide- (LPS-) induced mRNA and protein expression of inducible nitric oxide synthase and cyclooxygenase-2, as well as mRNA expression of tumor necrosis factor-alpha, interleukin- (IL-) 1β, and IL-6. Moreover, polyphenols inhibited nuclear translocation of NF-κB p65, phosphorylation/degradation of the inhibitor of κB, and phosphorylation of p38 MAPK, whereas the extracellular signal-regulated kinase and Janus N-terminal kinase were not affected. These results indicate that polyphenol components isolated from Korea L. japonica T. should have anti-inflammatory effect on LPS-stimulated RAW 264.7 cells through the decrease of proinflammatory mediators expression by suppressing NF-κB and p38 MAPK activity.
authors.affiliation: Research Institute of Life Science and College of Veterinary Medicine, Gyeongsang National University, Gazwa, Jinju 660-701, Republic of Korea.
authors.forename: Kwang-Il
authors.initials: KI
authors.lastname: Park
authors.forename: Sang-Rim
authors.initials: SR
authors.lastname: Kang
authors.forename: Hyeon-Soo
authors.initials: HS
authors.lastname: Park
authors.forename: Do Hoon
authors.initials: DH
authors.lastname: Lee
authors.forename: Arulkumar
authors.initials: A
authors.lastname: Nagappan
authors.forename: Jin A
authors.initials: JA
authors.lastname: Kim
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Kim
authors.forename: Won Sup
authors.initials: WS
authors.lastname: Lee
authors.forename: Hyon-Jong
authors.initials: HJ
authors.lastname: Chung
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: An
authors.forename: Gon Sup
authors.initials: GS
authors.lastname: Kim