title: Systems metabolic engineering of microorganisms for natural and non-natural chemicals.
abstract: Growing concerns over limited fossil resources and associated environmental problems are motivating the development of sustainable processes for the production of chemicals, fuels and materials from renewable resources. Metabolic engineering is a key enabling technology for transforming microorganisms into efficient cell factories for these compounds. Systems metabolic engineering, which incorporates the concepts and techniques of systems biology, synthetic biology and evolutionary engineering at the systems level, offers a conceptual and technological framework to speed the creation of new metabolic enzymes and pathways or the modification of existing pathways for the optimal production of desired products. Here we discuss the general strategies of systems metabolic engineering and examples of its application and offer insights as to when and how each of the different strategies should be used. Finally, we highlight the limitations and challenges to be overcome for the systems metabolic engineering of microorganisms at more advanced levels.
authors.affiliation: Department of Chemical and Biomolecular Engineering-Brain Korea 21 program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Jeong Wook
authors.initials: JW
authors.lastname: Lee
authors.forename: Dokyun
authors.initials: D
authors.lastname: Na
authors.forename: Jong Myoung
authors.initials: JM
authors.lastname: Park
authors.forename: Joungmin
authors.initials: J
authors.lastname: Lee
authors.forename: Sol
authors.initials: S
authors.lastname: Choi
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000235:genetics; D019020:Directed Molecular Evolution; D007218:Industrial Microbiology; D060847:Metabolic Engineering / Q000379:methods*; D009930:Organic Chemicals / Q000737:chemistry / Q000378:metabolism; D030781:Organisms, Genetically Modified; D058615:Synthetic Biology / Q000379:methods*; D049490:Systems Biology / Q000379:methods*
chemical_list: D009930:Organic Chemicals