title: Manual microdissection technique in a case of subcutaneous panniculitis-like T-cell lymphoma: a case report and review.
abstract: Demonstration of T-cell receptor gene monoclonality often plays an important role in the diagnosis of T-cell lymphoma. When a test to detect monoclonality is performed on whole tissue sections, the presence of a reactive lymphocyte population may reduce sensitivity. This may be especially true for early or borderline cases of lymphoma. Microdissection techniques may be utilized to more readily identify a clonal population of lymphocytes. Subcutaneous panniculitis-like T-cell lymphoma represents a cutaneous lymphoid neoplasm whose clinical course may vary from an indolent, waxing and waning course to an aggressive course resulting in death. We report the first case of a microdissection technique used to facilitate diagnosing a case of subcutaneous panniculitis-like T-cell lympoma.
authors.affiliation: Department of Dermatology, Henry Ford Hospital, Detroit, MI, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hoffman
authors.forename: Marsha
authors.initials: M
authors.lastname: Chaffins
authors.forename: Milena
authors.initials: M
authors.lastname: Cankovic
authors.forename: Koichi
authors.initials: K
authors.lastname: Maeda
authors.forename: Shane
authors.initials: S
authors.lastname: Meehan
mesh_terms: D002999:Clone Cells; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000235:genetics; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor / Q000235:genetics; D006801:Humans; D016410:Lymphoma, T-Cell, Cutaneous / Q000175:diagnosis* / Q000235:genetics / Q000601:surgery; D042282:Microdissection / Q000379:methods*; D008875:Middle Aged; D015434:Panniculitis / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D057089:Pathology, Molecular / Q000379:methods; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000601:surgery; D040521:Subcutaneous Tissue / Q000473:pathology / Q000601:surgery
chemical_list: D004273:DNA, Neoplasm