title: First report of a malignant collision skin tumour with malignant melanoma and anaplastic sarcoma components in a dog.
abstract: This paper describes the occurrence of a rare skin tumour that has been removed surgically from the upper lip of a 13-year-old Tibetan spaniel. The tumour was 0.5 cm in diameter and macroscopically appeared as a single dermal mass, but histopathological analysis identified it as a biphasic collision mixed tumour. In the anatomically uniform tumour, 70% (4 mm in diameter) of the total parenchyma was formed by a high-grade sarcoma (with the presence of giant cells), and about 30% of it (1 mm in diameter) was a malignant melanoma (again with the presence of giant cells). The histologically distinct, but anatomically uniform tumour parts were separated by a macroscopically invisible, non-neoplastic epithelial process originating from the overlying hyperplastic epidermis. The two malignant components did not infiltrate the peritumoural vessels and each other's substance. In the sarcoma part, the mitotic and apoptotic indexes were 32 and 8, respectively, whereas in the melanoma part the same parameters were 10 and 6, respectively. During the immunohistochemical investigations anti-α-SMA, anticytokeratin AE1-AE3, anti-Melan-A, anti-Ki-67 and anti-claudin-5 antibodies were applied. In conclusion, this is the first report of a primary cutaneous malignant biphasic collision mixed tumour formed by an anaplastic sarcoma with giant cells and a malignant melanoma.
authors.affiliation: Szent István University Department of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary Science István utca 2 H-1078 Budapest Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Jakab
authors.forename: Gyula
authors.initials: G
authors.lastname: Balka
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms; D062507:Claudin-5; D004285:Dogs; D007150:Immunohistochemistry; D008545:Melanoma; D012509:Sarcoma; D012878:Skin Neoplasms
chemical_list: D062507:Claudin-5