title: Comparative analysis of apoptotic resistance of mesenchymal stem cells isolated from human bone marrow and adipose tissue.
abstract: OBJECTIVE
  Mesenchymal stem cells (MSCs) isolated from human bone marrow (hBM) and adipose tissue (hAT) are perceived as attractive sources of stem cells for cell therapy. The aim of this study was to compare MSCs from hBM and hAT for their immunocytochemistry staining and resistance to in vitro apoptosis.
  
  
  METHODS
  In our study, we investigated the antiapoptotic ability of these MSCs toward oxidative stress induced by hydrogen peroxide (H(2)O(2)) and serum deprivation. Results were assessed by MTT and flow cytometry. All experiments were repeated a minimum of three times.
  
  
  RESULTS
  Flow cytometry and MTT analysis revealed that hAT-MSCs exhibited a higher resistance toward H(2)O(2)-induced apoptosis (n = 3, hBM-hAT viability H(2)O(2)  58.43 ± 1.24-73.02 ± 1.44, P < 0.02) and to serum-deprivation-induced apoptosis at days 1 and 4 than the hBM-MSCs (n = 3, hAT-hBM absorbance, resp., day 1: 0.305 ± 0.027-0.234 ± 0.015, P = 0.029, day 4: 0.355 ± 0.003-0.318 ± 0.007, P = 0.001, and day 7: 0.400 ± 0.017-0.356 ± 0.008, P = 0.672). hAT-MSCs showed superior tolerance to oxidative stress triggered by 2 mmol/L H(2)O(2) and also have superior antiapoptosis capacity toward serum-free culture.
  
  
  CONCLUSIONS
  In this study we found that hAT-MSCs are more resistant to in vitro apoptosis.
authors.affiliation: Department of Cardiology, Faculty of Medicine, Bezmialem Vakif University, Adnan Menderes Bulvarı Vatan Caddesi, Fatih, 34093 Istanbul, Turkey. drgokhanertas@yahoo.com.tr
authors.forename: Gökhan
authors.initials: G
authors.lastname: Ertaş
authors.forename: Ertan
authors.initials: E
authors.lastname: Ural
authors.forename: Dilek
authors.initials: D
authors.lastname: Ural
authors.forename: Ayça
authors.initials: A
authors.lastname: Aksoy
authors.forename: Güliz
authors.initials: G
authors.lastname: Kozdağ
authors.forename: Gülçin
authors.initials: G
authors.lastname: Gacar
authors.forename: Erdal
authors.initials: E
authors.lastname: Karaöz
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007150:Immunohistochemistry; D016130:Immunophenotyping; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018384:Oxidative Stress
chemical_list: D006861:Hydrogen Peroxide