title: Mycobacterium bovis BCG vaccine induces non-specific immune responses in Japanese flounder against Nocardia seriolae.
abstract: Nocardiosis caused by Nocardia seriolae has been causing severe loss of fish production, so that an effective vaccine is urgently needed. Mycobacterium bovis BCG (BCG) is a live attenuated vaccine for tuberculosis, which is effective against various infectious diseases including nocardiosis in mammals. In this study, the protective efficacy of BCG against N. seriolae was evaluated in Japanese flounder Paralichthys olivaceus and antigen-specific immune responses induced in BCG vaccinated fish were investigated. Cumulative mortality of BCG-vaccinated fish was 21.4% whereas that of PBS-injected fish was 56.7% in N. seriolae challenge. However, gene expression level of IFN-γ was only slightly up-regulated in BCG-vaccinated fish after injection of N. seriolae antigen. In order to reveal non-specific immune responses induced by BCG vaccination, transcriptome of the kidney after BCG vaccination was investigated using oligo DNA microarray. Gene expression levels of antimicrobial peptides such as C-type and G-type lysozyme were significantly up-regulated after BCG vaccination. Consistently, BCG vaccination appeared to increase the bacteriolysis activity of the serum against Micrococcus luteus and N. seriolae. These results suggest that BCG-vaccinated Japanese flounder fight N. seriolae infection mainly by non-specific immune responses such as by the production of bacteriolytic lysozymes.
authors.affiliation: Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, Japan.
authors.forename: Goshi
authors.initials: G
authors.lastname: Kato
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D015169:Colony Count, Microbial; D005393:Fish Diseases / Q000276:immunology* / Q000401:mortality; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology; D005432:Flounder / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007114:Immunization / Q000662:veterinary*; D009113:Muramidase / Q000235:genetics / Q000378:metabolism; D009163:Mycobacterium bovis / Q000276:immunology*; D009615:Nocardia / Q000276:immunology*; D009617:Nocardia Infections / Q000276:immunology / Q000401:mortality / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D001500:BCG Vaccine; D029941:Fish Proteins; D009113:Muramidase