title: High prevalence of bovine viral diarrhea virus 1 in Chinese swine herds.
abstract: Nested RT-PCR was used to investigate bovine viral diarrhea virus in 511 specimens collected from Chinese pigs exhibiting clinical symptoms between 2007 and 2010. Of these, 137 samples were BVDV-positive and the BVDV prevalence rate was 23.1% (9/39) in 2007, 27.7% (44/159) in 2008, 33.6% (34/101) in 2009, and 23.6% (50/212) in 2010. Twenty of 137 BVDV-positive samples were used for further genetic analysis of the 5'-UTR. Phylogenetic analysis revealed that they were BVDV-1 and subtyped into BVDV-1a, BVDV-1b, BVDV-1m, BVDV-1o and an unknown subgenotype. This study showed that BVDVs were highly prevalent in Chinese pig herds and appropriate measures should be taken to control BVDV prevalence in pig herds.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Deng
authors.forename: Chun-Qing
authors.initials: CQ
authors.lastname: Sun
authors.forename: San-Jie
authors.initials: SJ
authors.lastname: Cao
authors.forename: Tao
authors.initials: T
authors.lastname: Lin
authors.forename: Shi-Shan
authors.initials: SS
authors.lastname: Yuan
authors.forename: Hong-Biao
authors.initials: HB
authors.lastname: Zhang
authors.forename: Shao-Lun
authors.initials: SL
authors.lastname: Zhai
authors.forename: Lv
authors.initials: L
authors.lastname: Huang
authors.forename: Tong-Ling
authors.initials: TL
authors.lastname: Shan
authors.forename: Hao
authors.initials: H
authors.lastname: Zheng
authors.forename: Xin-Tian
authors.initials: XT
authors.lastname: Wen
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Tong
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology / Q000821:virology*; D002417:Cattle; D002681:China / Q000453:epidemiology; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification; D010802:Phylogeny; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*
chemical_list: D020121:5' Untranslated Regions