title: Regeneration of tracheal epithelium using a collagen vitrigel-sponge scaffold containing basic fibroblast growth factor.
abstract: OBJECTIVE
  Our group has had good results in tracheal mucosal regeneration using a collagen vitrigel-sponge scaffold in an animal model. In this study, the effectiveness of this scaffold with the application of basic fibroblast growth factor (b-FGF) was investigated.
  
  
  METHODS
  A collagen vitrigel-sponge scaffold was fabricated with simultaneous addition of b-FGF. Three types of collagen vitrigel-sponge scaffolds were made: no b-FGF, 10 ng of b-FGF, and 100 ng of b-FGF. At 3, 5, 7, and 14 days after implantation in rats, the tracheas were removed and histologically evaluated. The regeneration of mucosal epithelium and the subepithelial layer was evaluated.
  
  
  RESULTS
  Mucosal epithelium, including pseudostratified epithelium and ciliated cells, regenerated earlier in the scaffolds when b-FGF was applied than when b-FGF was not applied. Regeneration of the subepithelial layer, infiltration of inflammatory cells and fibroblasts, and angiogenesis were promoted earlier in the scaffolds with b-FGF application.
  
  
  CONCLUSIONS
  Our technique for tracheal reconstruction using collagen vitrigel-sponge scaffolds with b-FGF application affords a feasible approach for accelerating the regeneration of the intraluminal surface and subepithelial layer of tracheal tissue.
authors.affiliation: Department of Otolaryngology, Fukushima Medical University, Fukushima, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Tani
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tada
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Takezawa
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Imaizumi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nomoto
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Koichi
authors.initials: K
authors.lastname: Omori
mesh_terms: D000818:Animals; D003094:Collagen; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D005347:Fibroblasts / Q000166:cytology; D048091:Guided Tissue Regeneration / Q000379:methods*; D008297:Male; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D020545:Respiratory Mucosa / Q000098:blood supply / Q000473:pathology / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014132:Trachea / Q000098:blood supply / Q000473:pathology / Q000502:physiology*; D014140:Tracheotomy
chemical_list: D016222:Fibroblast Growth Factor 2; D003094:Collagen