title: Analytical challenges and technical limitations in assessing circulating miRNAs.
abstract: MiRNAs are emerging as promising biomarkers in cardiovascular diseases and may constitute a novel mechanism of intercellular communication. Accurate quantification of circulating miRNAs is essential. A variety of technological approaches and platforms have been developed with increased sensitivity and specificity for the detection and quantification of circulating miRNAs. In this review, we focus on the technical aspects and discuss the analytical challenges in profiling circulating miRNAs.
authors.affiliation: King's British Heart Foundation Centre, King's College London, London, UK. anna.zampetaki@kcl.ac.uk
authors.forename: Anna
authors.initials: A
authors.lastname: Zampetaki
authors.forename: Manuel
authors.initials: M
authors.lastname: Mayr
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000737:chemistry / Q000235:genetics*; D046210:Microfluidic Analytical Techniques / Q000379:methods; D015336:Molecular Probe Techniques; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009841:Oligonucleotides
chemical_list: D017931:DNA Primers; D035683:MicroRNAs; D009841:Oligonucleotides; C477371:locked nucleic acid