title: NFAT1 and NFAT2 differentially regulate IL-17A expression in human T cells.
abstract: BACKGROUND
  The NFAT family transcription factors play crucial roles in T cell functions. Recently, NFAT has been implicated in the production of an inflammatory cytokine, IL-17A; however, functional differences among NFAT members in IL-17A synthesis have not been elucidated. In this study, the relative contribution of NFAT1 and NFAT2 to IL-17A expression in human T cells was investigated.
  
  
  METHODS
  NFAT1 and NFAT2 were introduced in human cord blood CD4+ T cells by a lentiviral transduction system. Then, the expression of IL-17A mRNA was determined by quantitative real-time RT-PCR. The transient effects of NFAT1 and NFAT2 on IL-17A expression in Jurkat-Tag cells were also investigated.
  
  
  RESULTS
  Stimulation-induced expression of IL-17A in human CD4+ T cells was augmented by the introduction of NFAT1 and more vigorously, NFAT2. IL-17A expression in Jurkat-Tag cells was also enhanced by NFAT1, whereas it was not affected by NFAT2.
  
  
  CONCLUSIONS
  NFAT1 and NFAT2 facilitated IL-17A expression in human T cells, though distinct mechanisms might be involved in these effects.
authors.affiliation: Department of Allergy and Immunology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan. kaminuma-os@igakuken.or.jp
authors.forename: Osamu
authors.initials: O
authors.lastname: Kaminuma
authors.forename: Noriko
authors.initials: N
authors.lastname: Kitamura
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
authors.forename: Hideki
authors.initials: H
authors.lastname: Tatsumi
authors.forename: Soichi
authors.initials: S
authors.lastname: Nemoto
authors.forename: Takachika
authors.initials: T
authors.lastname: Hiroi
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics; D019169:Jurkat Cells; D050778:NFATC Transcription Factors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C508717:IL17A protein, human; D020381:Interleukin-17; D050778:NFATC Transcription Factors; D012333:RNA, Messenger