title: Lack of interference with immunogenicity of a chimeric alphavirus replicon particle-based influenza vaccine by preexisting antivector immunity.
abstract: Antivector immunity has been recognized as a potential caveat of using virus-based vaccines. In the present study, an alphavirus-based replicon particle vaccine platform, which has demonstrated robust immunogenicity in animal models, was tested for effects of antivector immunity on immunogenicity against hemagglutinin of influenza virus as a target antigen and efficacy for protection against lethal challenge with the virus. Chimeric alphavirus-based replicon particles, comprising Venezuelan equine encephalitis virus nonstructural and Sindbis virus structural components, induced efficient protective antibody responses, which were not adversely influenced after multiple immunizations with the same vector expressing various antigens.
authors.affiliation: Novartis Vaccines and Diagnostics S.r.l., Siena, Italy. yasushi.uematsu@novartis.com
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Uematsu
authors.forename: Michael
authors.initials: M
authors.lastname: Vajdy
authors.forename: Ying
authors.initials: Y
authors.lastname: Lian
authors.forename: Silvia
authors.initials: S
authors.lastname: Perri
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Greer
authors.forename: Harold S
authors.initials: HS
authors.lastname: Legg
authors.forename: Grazia
authors.initials: G
authors.lastname: Galli
authors.forename: Giulietta
authors.initials: G
authors.lastname: Saletti
authors.forename: Gillis R
authors.initials: GR
authors.lastname: Otten
authors.forename: Rino
authors.initials: R
authors.lastname: Rappuoli
authors.forename: Susan W
authors.initials: SW
authors.lastname: Barnett
authors.forename: John M
authors.initials: JM
authors.lastname: Polo
mesh_terms: D000524:Alphavirus / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004337:Drug Carriers; D005260:Female; D005822:Genetic Vectors / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000401:mortality; D016019:Survival Analysis; D014611:Vaccination / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D004337:Drug Carriers; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines