title: Tanshinone IIA prevents uric acid nephropathy in rats through NF-κB inhibition.
abstract: The purpose of this research is to investigate the effect of tanshinone IIA, an extract of the Chinese medicine Que Xie Hua Yu Tang, on uric acid nephropathy (UAN) and to elucidate the underlying mechanisms. UAN rat model was established. Fifty UAN rats were randomly allocated into 5 groups: adenine-treated group, allopurinol-treated group, and low/middle/high dose of tanshinone IIA-treated groups. Meanwhile, another 10 rats were used as normal controls. Serum uric acid (UA), blood urea nitrogen (BUN), serum creatinine (Scr), MCP-1, and IL-1β levels were measured. Histological staining was performed. Comparison between the adenine group and treatment (allopurinol and tanshinone IIA) groups showed compound treatment could attenuate the inflammation status of the kidneys and decrease serum UA levels. Among different kinds of medicine, tanshinone IIA had similar effects as allopurinol and exerted anti-inflammatory and renal protective effect in a dose-dependent manner. Furthermore, we found tanshinone IIA alone could also inhibit urate-induced MCP-1 and IL-1β overexpression both in vivo and in vitro, accompanied with inhibition of NF-κB translocation from cytosome to nucleus. Tanshinone IIA could protect rats from uric acid-induced kidney damage, probably by attenuating renal inflammatory status.
authors.affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. wangyaping4@gmail.com
authors.forename: Xinlin
authors.initials: X
authors.lastname: Wu
authors.forename: Lihua
authors.initials: L
authors.lastname: Liu
authors.forename: Hongbo
authors.initials: H
authors.lastname: Xie
authors.forename: Jiantang
authors.initials: J
authors.lastname: Liao
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Jianxin
authors.initials: J
authors.lastname: Wan
authors.forename: Kuang
authors.initials: K
authors.lastname: Yu
authors.forename: Junbiao
authors.initials: J
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000818:Animals; D001806:Blood Urea Nitrogen; D018932:Chemokine CCL2 / Q000097:blood; D003404:Creatinine / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D053583:Interleukin-1beta / Q000097:blood / Q000235:genetics; D007674:Kidney Diseases / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014527:Uric Acid / Q000097:blood*
chemical_list: D045784:Abietanes; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D016328:NF-kappa B; C021751:tanshinone; D014527:Uric Acid; D003404:Creatinine