title: In-gel staining of proteins in native polyacrylamide gel electrophoresis using meso-tetrakis(4-sulfonatophenyl) porphyrin.
abstract: Protein identification in polyacrylamide gel electrophoresis (PAGE) requires post-electrophoretic steps like fixing, staining, and destaining of the gel, which are time-consuming and cumbersome. A new method for direct visualization of protein bands in PAGE has been developed using meso-tetrakis(4-sulfonatophenyl)porphyrin (TPPS) as a dye without the need for any post-electrophoretic steps; thus, separation and recovery of enzymes become much easier for further analysis. Activity staining was carried out to show that the biochemical activity of the enzymes was preserved after electrophoresis.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, India.
authors.forename: K
authors.initials: K
authors.lastname: Divakar
authors.forename: G Nandhini
authors.initials: GN
authors.lastname: Devi
authors.forename: Pennathur
authors.initials: P
authors.lastname: Gautam
mesh_terms: D002021:Buffers; D004396:Coloring Agents / Q000138:chemical synthesis / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D057075:Enzyme Assays / Q000379:methods; D004798:Enzymes / Q000737:chemistry* / Q000302:isolation & purification; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry*; D011487:Protein Conformation; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D002021:Buffers; D004396:Coloring Agents; D004798:Enzymes; D011166:Porphyrins; C092400:meso-tetrakis(4-sulfonatophenyl)porphyrin