title: Ultrasensitive protein detection and imaging: comparison of Lumitein™, ProteoSilver™, SYPRO(®) Ruby, and Coomassie (®) Brilliant Blue gel stains.
abstract: Following sodium dodecyl sulfate polyacrylamide gel electrophoresis, proteins can be visualized by various methods of detection and imaging. Traditional methods of protein gel detection and imaging have been improved and expanded through technological advancement. Today, the detection of proteins, resolved on gels, can be accomplished with a variety of stains with various sensitivities. Digital cameras used in the imaging of protein gels are not only more sensitive than their film precursors, but they can be used in combination with imaging software that offers a host of useful applications. Here we describe the UVP BioImaging System in combination with LabWorks Image and Acquisition software to provide a comparison of four different protein gel stains: Lumitein™, ProteoSilver™, SYPRO(®) Ruby, and Coomassie(®) Brilliant Blue. We demonstrate that the detection sensitivity limit appears to be between 100 and 500 ng/protein band of protein with Coomassie(®) Brilliant Blue, 10-50 ng/protein band with Lumitein™ and SYPRO(®) Ruby, and as little as 5 ng/protein band with the ProteoSilver™ stain.
authors.affiliation: Boshell Diabetes and Metabolic Diseases Research Program, Auburn University, Auburn, AL, USA.
authors.forename: Guang
authors.initials: G
authors.lastname: Ren
authors.forename: Carl K
authors.initials: CK
authors.lastname: Okerberg
authors.forename: Suresh T
authors.initials: ST
authors.lastname: Mathews
mesh_terms: D000818:Animals; D002021:Buffers; D002417:Cattle; D004396:Coloring Agents / Q000737:chemistry*; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000592:standards; D005456:Fluorescent Dyes / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D057230:Limit of Detection; D009942:Organometallic Compounds / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D012015:Reference Standards; D012394:Rosaniline Dyes / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification; D012835:Silver Nitrate / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods / Q000592:standards
chemical_list: D002021:Buffers; D004396:Coloring Agents; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D011506:Proteins; D012394:Rosaniline Dyes; C515601:Sypro Ruby; D012710:Serum Albumin, Bovine; D012835:Silver Nitrate; C004692:coomassie Brilliant Blue