title: The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis.
abstract: We isolated Arabidopsis thaliana mutants with incurved vegetative leaves. Positional cloning of incurvata8 (icu8), icu9 and icu15 has identified them as new loss-of-function alleles of the HYPONASTIC LEAVES1 (HYL1), ARGONAUTE1 (AGO1) and HUA ENHANCER1 (HEN1) genes, respectively, which encode known components of the microRNA pathway. The morphological and histological characterization of these mutants and of dicer-like1-9 indicates that small RNAs participate in the proximal-distal and adaxial-abaxial patterning of leaves, as well as in stomatal number establishment. The abnormal vasculature of ago1 and hyl1 leaves also suggests a role for AGO1 and HYL1 in venation patterning. Our mutants expand the allelic series of AGO1, HYL1 and HEN1, and might help to understand the developmental and cellular significance of miRNA-mediated posttranscriptional regulation.
authors.affiliation: Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche, 03202 Elche, Alicante, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Jover-Gil
authors.forename: Héctor
authors.initials: H
authors.lastname: Candela
authors.forename: Pedro
authors.initials: P
authors.lastname: Robles
authors.forename: Verónica
authors.initials: V
authors.lastname: Aguilera
authors.forename: José María
authors.initials: JM
authors.lastname: Barrero
authors.forename: José Luis
authors.initials: JL
authors.lastname: Micol
authors.forename: María Rosa
authors.initials: MR
authors.lastname: Ponce
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010641:Phenotype; D019441:Plant Epidermis / Q000378:metabolism / Q000502:physiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D054046:Plant Stomata / Q000235:genetics / Q000502:physiology*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism
chemical_list: C117457:AGO1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D060565:Argonaute Proteins; D018797:Cell Cycle Proteins; C450825:HEN1 protein, Arabidopsis; C420966:HYL1 protein, Arabidopsis; D035683:MicroRNAs; D018749:RNA, Plant; D016601:RNA-Binding Proteins; C402383:DCL1 protein, Arabidopsis; D043244:Ribonuclease III