title: Identification of caerulomycin A gene cluster implicates a tailoring amidohydrolase.
abstract: The biosynthetic gene cluster for caerulomycin A (1) was cloned and characterized from the marine actinomycete Actinoalloteichus cyanogriseus WH1-2216-6, which revealed an unusual hybrid polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) system. The crmL disruption mutant accumulated caerulomycin L (2) with an extended L-leucine at C-7, implicating an amidohydrolase activity for CrmL. The leucine-removing activity was confirmed for crude CrmL enzymes. Heterologous expression of the 1 gene cluster led to 1 production in Streptomyces coelicolor.
authors.affiliation: CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
authors.forename: Yiguang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Peng
authors.initials: P
authors.lastname: Fu
authors.forename: Qinheng
authors.initials: Q
authors.lastname: Lin
authors.forename: Guangtao
authors.initials: G
authors.lastname: Zhang
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhang
authors.forename: Sumei
authors.initials: S
authors.lastname: Li
authors.forename: Jianhua
authors.initials: J
authors.lastname: Ju
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhu
authors.forename: Changsheng
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000192:Actinomycetales / Q000235:genetics* / Q000378:metabolism; D000581:Amidohydrolases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D005798:Genes, Bacterial / Q000235:genetics; D007930:Leucine / Q000378:metabolism; D015394:Molecular Structure; D005810:Multigene Family; D010453:Peptide Synthases / Q000378:metabolism; D011725:Pyridines / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011725:Pyridines; C544423:caerulomycin A; D000581:Amidohydrolases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase; D007930:Leucine