title: Mode-dependent effect of high-frequency electrical stimulation of the anterior thalamic nucleus on amygdala-kindled seizures in rats.
abstract: Deep brain stimulation (DBS) is an emerging treatment of epilepsy. Anterior nucleus of the thalamus (ANT) is considered to be an attractive target due to its close connection to the limbic structures and wide regions of neocortex. The present study aimed to investigate the effects of high frequency stimulation (HFS) targeting the ANT on amygdala-kindled seizures in Wistar rats in two different stimulation modes i.e. pre-treatment and post-treatment stimulations, mimicking the scheduled and responsive stimulations in clinical use respectively. When fully-kindled seizures were achieved by daily amygdala kindling (1 s train of 1 ms pulses at 60 Hz), HFS (15 min train of 100 μs pulses at 150 Hz and 450-800 μA) was applied in two modes for 10 days. Bilateral post-treatment with HFS reduced the incidence of generalized seizures and the mean behavioral seizure stage and shortened average afterdischarge duration (ADD) and generalized seizure duration (GSD), while bilateral pre-treatment with HFS resulted in a similar but much weaker inhibition of seizures. On the other hand, we also found the two stimulation modes both increased the afterdischarge threshold (ADT) and the differences of current intensity between ADT and generalized seizure threshold (GST) i.e. Δ(GST-ADT). However, Δ(GST-ADT) increased by at least 20 μA in bilateral post-treatment group, while less in bilateral pre-treatment group. Additionally, unilateral post-treatment with HFS failed to inhibit seizures. Our data show that anti-epileptic effect of bilateral post-treatment with HFS of ANT is much stronger than that of bilateral pre-treatment HFS, indicating bilateral responsive stimulation might be more appropriate for clinical anti-epileptic treatment of ANT HFS.
authors.affiliation: Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao 266071, PR China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: Z C
authors.initials: ZC
authors.lastname: Wu
authors.forename: J-T
authors.initials: JT
authors.lastname: Yu
authors.forename: N N
authors.initials: NN
authors.lastname: Yu
authors.forename: X L
authors.initials: XL
authors.lastname: Zhong
authors.forename: L
authors.initials: L
authors.lastname: Tan
mesh_terms: D000679:Amygdala / Q000503:physiopathology*; D000818:Animals; D020643:Anterior Thalamic Nuclei / Q000503:physiopathology*; D046690:Deep Brain Stimulation / Q000379:methods*; D004558:Electric Stimulation / Q000379:methods*; D007696:Kindling, Neurologic / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000503:physiopathology / Q000628:therapy*; D016896:Treatment Outcome