title: Development of novel chloroplast microsatellite markers for Miscanthus species (Poaceae).
abstract: OBJECTIVE
  A set of novel chloroplast microsatellite markers (cpSSRs) was developed for the bioenergy crop Miscanthus, and their utility in cross-species amplification was evaluated.
  
  
  RESULTS
  Twenty-eight novel primers flanking cpSSR loci were designed from a complete chloroplast genome sequence of Saccharum officinarum, a species closely related to Miscanthus. These primers were then tested on eight Miscanthus species, among which 16 cpSSR loci were found to be polymorphic. The number of alleles per polymorphic locus ranged from two to seven, with an average 3.94 alleles.
  
  
  CONCLUSIONS
  These cpSSR markers can be applied to all Miscanthus species and will be useful for studying Miscanthus population structure, diversity, and phylogeography.
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan, Changsha 410128, People's Republic of China.
authors.forename: Jian-Xiong
authors.initials: JX
authors.lastname: Jiang
authors.forename: Zu-Hong
authors.initials: ZH
authors.lastname: Wang
authors.forename: Bing-Rong
authors.initials: BR
authors.lastname: Tang
authors.forename: Liang
authors.initials: L
authors.lastname: Xiao
authors.forename: Xin
authors.initials: X
authors.lastname: Ai
authors.forename: Zi-Li
authors.initials: ZL
authors.lastname: Yi
mesh_terms: D000483:Alleles; D002736:Chloroplasts / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018742:DNA, Chloroplast / Q000737:chemistry / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D058974:Phylogeography / Q000379:methods; D006109:Poaceae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D031786:Saccharum / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018742:DNA, Chloroplast; D018744:DNA, Plant