title: HCS-Analyzer: open source software for high-content screening data correction and analysis.
abstract: BACKGROUND
  High-throughput screening is a powerful technology principally used by pharmaceutical industries allowing the identification of molecules of interest within large libraries. Originally target based, cellular assays provide a way to test compounds (or other biological material such as small interfering RNA) in a more physiologically realistic in vitro environment. High-content screening (HCS) platforms are now available at lower cost, giving the opportunity for universities or research institutes to access those technologies for research purposes. However, the amount of information extracted from each experiment is multiplexed and hence difficult to handle. In such context, there is an important need for an easy-to-use, but still powerful software able to manage multidimensional screening data by performing adapted quality control and classification. HCS-analyzer includes: a user-friendly interface specifically dedicated to HCS readouts, an automated approach to identify systematic errors potentially occurring during screening and a set of tools to classify, cluster and identify phenotypes of interest among large and multivariate data.
  
  
  BACKGROUND
  The application, the C# .Net source code, as well as detailed documentation, are freely available at the following URL: http://hcs-analyzer.ip-korea.org.
authors.affiliation: Cellular Differentiation and Toxicity Prediction, Institut Pasteur Korea 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400 Korea.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Ogier
authors.forename: Thierry
authors.initials: T
authors.lastname: Dorval
mesh_terms: D000465:Algorithms; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods*; D004345:Drug Industry; D057166:High-Throughput Screening Assays; D034741:RNA, Small Interfering; D012984:Software; D014584:User-Computer Interface
chemical_list: D034741:RNA, Small Interfering