title: Convenient and efficient enrichment of the CD133+ liver cells from rat fetal liver as a source of liver stem/progenitor cells.
abstract: Although stem cells are commonly isolated by fluorescence-activated cell sorting or magnetic affinity cell sorting, they are very expensive, and they need known markers. However, there is no specific marker for liver stem/progenitor cells (LSPCs). Here, we describe a convenient and efficient method (three-step method) to enrich LSPCs. The fetal liver cells (FLCs) were firstly enriched by Percoll discontinuous gradient centrifugation from the rat fetal liver. Then the FLCs in culture were purified to be homogeneous in size by differential trypsinization and differential adherence. Finally, fetal liver stem/progenitor cells (FLSPCs) were enriched from purified FLCs by Percoll continuous gradient centrifugation. Flow cytometric analysis combining with marker CD133 was used to detect the purity of FLSPCs and evaluate the isolating effects of the three-step method.
authors.affiliation: Department of Hepatobiliary Surgery, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
authors.forename: Weihui
authors.initials: W
authors.lastname: Liu
authors.forename: Nan
authors.initials: N
authors.lastname: You
authors.forename: Kefeng
authors.initials: K
authors.lastname: Dou
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods*; D005333:Fetus / Q000166:cytology*; D006023:Glycoproteins / Q000378:metabolism*; D008099:Liver / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D013234:Stem Cells / Q000166:cytology*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D010455:Peptides; C000606025:Prom1 protein, rat