title: Separating and analyzing sulfur-containing RNAs with organomercury gels.
abstract: Polyacrylamide gel electrophoresis is a widely used technique for RNA analysis and purification. The polyacrylamide matrix is highly versatile for chemical derivitization, enabling facile exploitation of thio-mercury chemistry without the need of tedious manipulations and/or expensive coupling reagents, which often give low yields and side products. Here, we describe the use of [(N-acryloylamino)phenyl]mercuric chloride in three-layered polyacrylamide gels to detect, separate, quantify, and analyze sulfur-containing RNAs.
authors.affiliation: Department of Molecular Microbiology and Immunology and Department of Biochemistry, University of Missouri-School of Medicine, Columbia, MO, USA. biondie@missouri.edu
authors.forename: Elisa
authors.initials: E
authors.lastname: Biondi
authors.forename: Donald H
authors.initials: DH
authors.lastname: Burke
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D002021:Buffers; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005782:Gels; D010663:Phenylmercury Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D054735:Phosphorothioate Oligonucleotides / Q000737:chemistry / Q000302:isolation & purification*; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000180:Acrylic Resins; D002021:Buffers; D003432:Cross-Linking Reagents; D005782:Gels; D010663:Phenylmercury Compounds; D054735:Phosphorothioate Oligonucleotides; C016680:polyacrylamide gels; D012313:RNA; C056922:((N-acryloylamino)phenyl)mercuric chloride