title: Myoblast cell interaction with polydopamine coated liposomes.
abstract: Liposomes are widely used, from biosensing to drug delivery. Their coating with polymers for stability and functionalization purposes further broadens their set of relevant properties. Poly(dopamine) (PDA), a eumelanin-like material deposited via the "self"-oxidative polymerization of dopamine at mildly basic pH, has attracted considerable interest in the past few years due to its simplicity, flexibility yet fascinating properties. Herein, we characterize the coating of different types of liposomes with PDA depending on the presence of oleoyldopamine in the lipid bilayer and the dopamine hydrochloride concentration. Further, the interaction of these coated liposomes in comparison to their uncoated counterparts with myoblast cells is assessed. Their uptake/association efficiency with these cells is determined. Further, their dose-dependent cytotoxicity with and without entrapped hydrophobic cargo (thiocoraline) is characterized. Taken together, the reported results demonstrate the potential of PDA coated liposomes as a tool in biomedical applications.
authors.affiliation: iNANO Interdisciplinary Nanoscience Centre, Aarhus University, Aarhus, Denmark.
authors.forename: Rebecca
authors.initials: R
authors.lastname: van der Westen
authors.forename: Leticia
authors.initials: L
authors.lastname: Hosta-Rigau
authors.forename: Duncan S
authors.initials: DS
authors.lastname: Sutherland
authors.forename: Kenneth N
authors.initials: KN
authors.lastname: Goldie
authors.forename: Fernando
authors.initials: F
authors.lastname: Albericio
authors.forename: Almar
authors.initials: A
authors.lastname: Postma
authors.forename: Brigitte
authors.initials: B
authors.lastname: Städler
mesh_terms: D000818:Animals; D002450:Cell Communication; D002460:Cell Line; D004705:Endocytosis; D007211:Indoles / Q000737:chemistry* / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D032446:Myoblasts / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D007211:Indoles; D008081:Liposomes; D011108:Polymers; C568283:polydopamine