title: Flavonoid-deficient mutants in grass pea (Lathyrus sativus L.): genetic control, linkage relationships, and mapping with aconitase and S-nitrosoglutathione reductase isozyme loci.
abstract: Two flavonoid-deficient mutants, designated as fldL-1 and fldL-2, were isolated in EMS-mutagenized (0.15%, 10 h) M(2) progeny of grass pea (Lathyrus sativus L.). Both the mutants contained total leaf flavonoid content only 20% of their mother varieties. Genetic analysis revealed monogenic recessive inheritance of the trait, controlled by two different nonallelic loci. The two mutants differed significantly in banding patterns of leaf aconitase (ACO) and S-nitrosoglutathione reductase (GSNOR) isozymes, possessing unique bands in Aco 1, Aco 2, and Gsnor 2 loci. Isozyme loci inherited monogenically showing codominant expression in F(2) (1:2:1) and backcross (1:1) segregations. Linkage studies and primary trisomic analysis mapped Aco 1 and fld 1 loci on extra chromosome of trisomic-I and Aco 2, fld 2, and Gsnor 2 on extra chromosome of trisomic-IV in linked associations.
authors.affiliation: Department of Botany, RPM College, University of Calcutta, Uttarpara, West Bengal, Hooghly 712 258, India. dibyendutalukdar9@gmail.com
authors.forename: Dibyendu
authors.initials: D
authors.lastname: Talukdar
mesh_terms: D000154:Aconitate Hydratase / Q000235:genetics / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D005419:Flavonoids / Q000378:metabolism*; D005808:Genes, Recessive; D008040:Genetic Linkage; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D027807:Lathyrus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D014314:Trisomy
chemical_list: D005419:Flavonoids; D007527:Isoenzymes; D010940:Plant Proteins; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent; D000154:Aconitate Hydratase