title: [Effects of 1,25(OH)(2)D(3) on SGC-7901 cell proliferation and tumor necrosis factor-α expression].
abstract: OBJECTIVE
  To investigate the effect of 1,25 (OH)(2)D(3) on cell proliferation and the expression of tumor necrosis factor-α  (TNF-α ) in human gastric carcinoma SGC-7901 cells.
  
  
  METHODS
  SGC-7901 cells were treated with 1×10(-6), 1×10(-7), 1×10(-8), and 1×10(-9) mol/L 1,25 (OH)(2)D(3) for 24, 48, 72 and 96 h. The cell proliferation was measured by MTT assay, and the cell cycle changes were analyzed using flow cytometry. RT-PCR and Western blotting were used to determine the expression of TNF-α mRNA and protein, respectively.
  
  
  RESULTS
  1,25 (OH)(2)D(3) significantly inhibited SGC-7901 cell proliferation (P<0.05) in a time- and dose-dependent fashion. Treatment with 1,25 (OH)(2)D(3) for 72 h caused significant cell cycle arrest at G(0)/G(1) phase  (F=9.81, P<0.05) and dose-dependently inhibited the expression of TNF-α at both mRNA and protein levels in SGC-7901 cells (P<0.05).
  
  
  CONCLUSIONS
  The inhibitory effect of 1,25 (OH)(2)D(3) on SGC-7901 cell proliferation is probably associated with the down- regulation of TNF-α expression.
authors.affiliation: Department of Gastroenterology, Chongqing Medical University, Chongqing, China. zl-1125@qq.com
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Lulu
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wu
mesh_terms: D002117:Calcitriol / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D002117:Calcitriol