title: cDNA cloning, characterization and expression of lipopolysaccharide and β-1,3-glucan binding protein (LGBP) gene from the Indian white shrimp Fenneropenaeus indicus.
abstract: A 1372bp full length cDNA of an LGBP gene was identified from the Indian white shrimp, Fenneropenaeus indicus. The open reading frame (ORF) of the F. indicus LGBP (Fein-LGBP) was 1164bp long, encoding a polypeptide of 388 amino acids. The Fein-LGBP deduced amino acid has conserved potential recognition motif for β-1, 3 linkages of polysaccharides and putative RGD (Arg-Gly-Asp) cell adhesion sites. Phylogenetic analysis revealed that the Fein-LGBP was grouped together with LGBPs of other crustaceans such as F. chinensis (91%, AAZ41363), Penaeus monodon (88%, AAM21213) and Litopenaeus vannamei (88%, ABU92557). Quantitative RT-PCR results showed that Fein-LGBP gene expression was up-regulated in hemocytes following a 6h challenge in response to peptidoglycan (PG) and Vibrio parahaemolyticus. Messenger RNA transcripts of Fein-LGBP were measured in hemocytes of F. indicus from different molting stages. The highest Fein-LGBP mRNA expression was observed in pre-molting stages (D0/1) when compared to post-molt stages (A and B) and inter-molt stages (C). Fein-LGBP is involved in the regulation and activation of the prophenoloxidase cascade.
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
authors.forename: J Sivakama
authors.initials: JS
authors.lastname: Valli
authors.forename: B
authors.initials: B
authors.lastname: Vaseeharan
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics*; D020869:Gene Expression Profiling; D007194:India; D037102:Lectins / Q000235:genetics*; D008070:Lipopolysaccharides / Q000378:metabolism*; D033561:Penaeidae / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D037102:Lectins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; C037937:glucan-binding proteins