title: Bio-functionalization of ligand-free upconverting lanthanide doped nanoparticles for bio-imaging and cell targeting.
abstract: We report on the functionalization of ligand-free NaGdF(4):Er(3+), Yb(3+) upconverting nanoparticles with heparin and basic fibroblast growth factor (bFGF). These upconverting nanoparticles are used to obtain high-contrast images of HeLa cells. These images reveal that the heparin-bFGF functionalized nanoparticles show specific binding to the cell membrane.
authors.affiliation: Department of Chemistry and Biochemistry and Centre for Research in NanoScience, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, Canada.
authors.forename: Nicoleta
authors.initials: N
authors.lastname: Bogdan
authors.forename: Emma Martín
authors.initials: EM
authors.lastname: Rodríguez
authors.forename: Francisco
authors.initials: F
authors.lastname: Sanz-Rodríguez
authors.forename: Ma Carmen Iglesias
authors.initials: MC
authors.lastname: de la Cruz
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Juarranz
authors.forename: Daniel
authors.initials: D
authors.lastname: Jaque
authors.forename: José García
authors.initials: JG
authors.lastname: Solé
authors.forename: John A
authors.initials: JA
authors.lastname: Capobianco
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D003287:Contrast Media / Q000737:chemistry* / Q000633:toxicity; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006367:HeLa Cells; D006493:Heparin / Q000378:metabolism; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008024:Ligands; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D008856:Microscopy, Fluorescence; D011485:Protein Binding
chemical_list: D003287:Contrast Media; D028581:Lanthanoid Series Elements; D008024:Ligands; D016222:Fibroblast Growth Factor 2; D006493:Heparin