title: Effective separation of dopamine from bananas on 2-(3,4-dimethoxyphenyl)ethylamine imprinted polymer.
abstract: A 2-(3,4-dimethoxyphenyl)ethylamine imprinted polymer (MIP(pt) ) was prepared via the precipitation polymerization together with a nonimprinted polymer (NIP). The morphology of particles was investigated by scanning electron microscopy and the specific surface areas were estimated by methylene blue adsorption (60.5 ± 3.5 and 36.9 ± 1.2 m(2)/g for MIP(pt) and NIP, respectively). The binding experiments were performed to determine the binding capacity of MIP(pt)/NIP particles toward dopamine. Next, the effects of solvents on loading, washing, and eluting steps were examined on solid-phase extraction (SPE). Methanol-water 85:15 v/v (loading step), methanol (washing step), and 0.04 M aqueous ammonium acetate-methanol 30:70 v/v (eluting step) were selected as the most effective systems. Described SPE protocol was successfully applied for separation of dopamine on 2-(3,4-dimethoxyphenyl)ethylamine imprinted particles. Finally, the molecularly imprinted polymer was used for determination of dopamine in spiked banana extract. The total recovery of dopamine from MIP(pt) was equal to 88.5 ± 4.6%, but from NIP was only 12.8 ± 2.3%. The developed material and method were demonstrated to be applicable for the separation of dopamine from bananas. The commercial sorbent C18 was not suitable to such application.
authors.affiliation: Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Luliński
authors.forename: Dorota
authors.initials: D
authors.lastname: Maciejewska
mesh_terms: D000327:Adsorption; D004298:Dopamine / Q000032:analysis / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis; D054802:Molecular Imprinting; D028521:Musa / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D011108:Polymers; D004298:Dopamine