title: A simple strand-specific RNA-Seq library preparation protocol combining the Illumina TruSeq RNA and the dUTP methods.
abstract: Preserving the original RNA orientation information in RNA-Sequencing (RNA-Seq) experiment is essential to the analysis and understanding of the complexity of mammalian transcriptomes. We describe herein a simple, robust, and time-effective protocol for generating strand-specific RNA-seq libraries suited for the Illumina sequencing platform. We modified the Illumina TruSeq RNA sample preparation by implementing the strand specificity feature using the dUTP method. This protocol uses low amounts of starting material and allows a fast processing within two days. It can be easily implemented and requires only few additional reagents to the original Illumina kit.
authors.affiliation: Max Planck Institute for Molecular Genetics, Ihnestr. 73, D-14195 Berlin, Germany. sultan@molgen.mpg.de
authors.forename: Marc
authors.initials: M
authors.lastname: Sultan
authors.forename: Simon
authors.initials: S
authors.lastname: Dökel
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Amstislavskiy
authors.forename: Daniela
authors.initials: D
authors.lastname: Wuttig
authors.forename: Holger
authors.initials: H
authors.lastname: Sültmann
authors.forename: Hans
authors.initials: H
authors.lastname: Lehrach
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Yaspo
mesh_terms: D003856:Deoxyuracil Nucleotides / Q000737:chemistry* / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D006801:Humans; D017423:Sequence Analysis, RNA / Q000379:methods*; D059467:Transcriptome
chemical_list: D003856:Deoxyuracil Nucleotides