title: A functional pectin methylesterase inhibitor protein (SolyPMEI) is expressed during tomato fruit ripening and interacts with PME-1.
abstract: A pectin methylesterase inhibitor (SolyPMEI) from tomato has been identified and characterised by a functional genomics approach. SolyPMEI is a cell wall protein sharing high similarity with Actinidia deliciosa PMEI (AdPMEI), the best characterised inhibitor from kiwi. It typically affects the activity of plant pectin methylesterases (PMEs) and is inactive against a microbial PME. SolyPMEI transcripts were mainly expressed in flower, pollen and ripe fruit where the protein accumulated at breaker and turning stages of ripening. The expression of SolyPMEI correlated during ripening with that of PME-1, the major fruit specific PME isoform. The interaction of SolyPMEI with PME-1 was demonstrated in ripe fruit by gel filtration and by immunoaffinity chromatography. The analysis of the zonal distribution of PME activity and the co-localization of SolyPMEI with high esterified pectins suggest that SolyPMEI regulates the spatial patterning of distribution of esterified pectins in fruit.
authors.affiliation: Dipartimento di Biologia e Biotecnologie "Charles Darwin", "Sapienza" Università di Roma, Piazzale Aldo Moro 5, 00185, Rome, Italy.
authors.forename: Ida Barbara
authors.initials: IB
authors.lastname: Reca
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Lionetti
authors.forename: Laura
authors.initials: L
authors.lastname: Camardella
authors.forename: Rossana
authors.initials: R
authors.lastname: D'Avino
authors.forename: Thierry
authors.initials: T
authors.lastname: Giardina
authors.forename: Felice
authors.initials: F
authors.lastname: Cervone
authors.forename: Daniela
authors.initials: D
authors.lastname: Bellincampi
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D004791:Enzyme Inhibitors / Q000378:metabolism; D004951:Esterification; D005638:Fruit / Q000254:growth & development / Q000378:metabolism; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D010368:Pectins / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D018744:DNA, Plant; D004791:Enzyme Inhibitors; C412789:PMEI protein, Actinidia chinensis; D010940:Plant Proteins; D011994:Recombinant Proteins; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase