title: Strong and selective binding of amiloride to an abasic site in RNA duplexes: thermodynamic characterization and microRNA detection.
abstract: Firmly tied: The binding affinity of amiloride for an abasic (AP) site-containing RNA duplex is two orders of magnitude superior to the affinity of the corresponding AP site-containing DNA duplex. The observed high binding affinity for the RNA duplex arises from a favorable enthalpy gain. The binding-induced fluorescence response of amiloride is applicable to microRNA detection.
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Toshiki
authors.initials: T
authors.lastname: Ichihashi
authors.forename: Seiichi
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Norio
authors.initials: N
authors.lastname: Teramae
mesh_terms: D000584:Amiloride / Q000737:chemistry*; D001483:Base Sequence; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D035683:MicroRNAs; D012313:RNA; D000584:Amiloride