title: PHD finger of the SUMO ligase Siz/PIAS family in rice reveals specific binding for methylated histone H3 at lysine 4 and arginine 2.
abstract: We determined the three-dimensional structure of the PHD finger of the rice Siz/PIAS-type SUMO ligase, OsSiz1, by NMR spectroscopy and investigated binding ability for a variety of methylated histone H3 tails, showing that OsSiz1-PHD primarily recognizes dimethylated Arg2 of the histone H3 and that methylations at Arg2 and Lys4 reveal synergy effect on binding to OsSiz1-PHD. The K4 cage of OsSiz1-PHD for trimethylated Lys4 of H3K4me3 was similar to that of the BPTF-PHD finger, while the R2 pocket for Arg2 was different. It is intriguing that the PHD module of Siz/PIAS plays an important role, with collaboration with the DNA binding domain SAP, in gene regulation through SUMOylation of a variety of effectors associated with the methylated arginine-riched chromatin domains.
authors.affiliation: Biomolecular Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: Heisaburo
authors.initials: H
authors.lastname: Shindo
authors.forename: Rintaro
authors.initials: R
authors.lastname: Suzuki
authors.forename: Wataru
authors.initials: W
authors.lastname: Tsuchiya
authors.forename: Misako
authors.initials: M
authors.lastname: Taichi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nishiuchi
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Yamazaki
mesh_terms: D001120:Arginine / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D008025:Ligases / Q000737:chemistry* / Q000378:metabolism; D008239:Lysine / Q000235:genetics* / Q000378:metabolism; D008745:Methylation; D008958:Models, Molecular; D012275:Oryza / Q000201:enzymology* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D058207:Sumoylation
chemical_list: D006657:Histones; D010940:Plant Proteins; D001120:Arginine; D008025:Ligases; D008239:Lysine