title: Microsatellite primers for the endangered aquatic herb, Ottelia acuminata (Hydrocharitaceae).
abstract: OBJECTIVE
  Microsatellite primers were developed in the endangered aquatic herb, Ottelia acuminata, to characterize its genetic diversity and understand its population structure.
  
  
  RESULTS
  Eight polymorphic microsatellite markers were developed from two populations of O. acuminata in China. The number of alleles per locus ranged from one to 15; the observed and expected heterozygosities ranged from 0 to 0.885 and from 0 to 0.888, respectively, in the two populations. Selected loci also amplified successfully in O. sinensis.
  
  
  CONCLUSIONS
  These microsatellite markers will facilitate further studies on the conservation genetics and evolutionary history of O. acuminata.
authors.affiliation: Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Du
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Chen
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Wang
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D056727:Endangered Species; D005838:Genotype; D031321:Hydrocharitaceae / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant