title: A crucial role for bone morphogenetic protein-Smad1 signalling in the DNA damage response.
abstract: DNA damage and the elicited cellular response underlie the etiology of tumorigenesis and ageing. Yet, how this response integrates inputs from cells' environmental cues remains underexplored. Here we report that the BMP-Smad1 pathway, which is essential for embryonic development and tissue homeostasis, has an important role in the DNA damage response and oncogenesis. On genotoxic stress, Atm phosphorylates BMPs-activated Smad1 in the nucleus on S239, which disrupts Smad1 interaction with protein phosphatase PPM1A, leading to enhanced activation and upregulation of Smad1. Smad1 then interacts with p53 and inhibits Mdm2-mediated p53 ubiquitination and degradation to regulate cell proliferation and survival. Enhanced Smad1 S239 phosphorylation, and Smad1 mutations causing S239 substitution were detected in oesophageal and gastric cancer samples, respectively. These findings suggest that BMP-Smad1 signalling participates in the DNA damage response via the Atm-p53 pathway, thus providing a molecular mechanism whereby BMP-Smad1 loss-of-function leads to tumorigenesis, for example, juvenile polyposis and Cowden syndromes.
authors.affiliation: The Institute of Molecular and Cell Biology, Singapore 138673.
authors.forename: Jenny Fung Ling
authors.initials: JF
authors.lastname: Chau
authors.forename: Deyong
authors.initials: D
authors.lastname: Jia
authors.forename: Zhongfeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Yuanyu
authors.initials: Y
authors.lastname: Hu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Jia
authors.forename: Keng Po
authors.initials: KP
authors.lastname: Lai
authors.forename: Wai Fook
authors.initials: WF
authors.lastname: Leong
authors.forename: Bi Jin
authors.initials: BJ
authors.lastname: Au
authors.forename: Yuji
authors.initials: Y
authors.lastname: Mishina
authors.forename: Ye-Guang
authors.initials: YG
authors.lastname: Chen
authors.forename: Christine
authors.initials: C
authors.lastname: Biondi
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Robertson
authors.forename: Dong
authors.initials: D
authors.lastname: Xie
authors.forename: Huijuan
authors.initials: H
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Xueying
authors.initials: X
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yu
authors.forename: Baojie
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008819:Mice, Nude; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D051898:Smad1 Protein / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D019485:Bone Morphogenetic Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D051898:Smad1 Protein; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; C576325:Atm protein, mouse; D017346:Protein Serine-Threonine Kinases