title: Study on the immunomodulation effect of Isodon japonicus extract via splenocyte function and NK anti-tumor activity.
abstract: Here we investigated the potential immune-enhancing activity of Isodon japonicus on murine splenocyte and natural-killer (NK) cells in vitro. The ethanol extract of I. japonicus significantly enhanced the proliferation of splenocyte and induced the significant enhancement of NK cells' activity against tumor cells (YAC-1). In addition, I. japonicus increased the production of interferon (IFN)-γ and tumor necrosis factor (TNF)-α, suggesting that the increase in NK cell cytotoxicity could be due to the enhancement of the NK cell production of both cytokines. Taken together, I. japonicus extract inhibited the growth of human leukemia cells (K562) by 74%. Our observation indicated that the anti-tumor effects of I. japonicus may be attributed to its ability to serve as a stimulant of NK anti-tumor activity. In addition, our results support the development of functional food studies on I. japonicus.
authors.affiliation: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea.|Department of Biotechnology & Bioengineering, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Hwang
authors.affiliation: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea.
authors.forename: Jaehyun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea.
authors.forename: Dong-Sik
authors.initials: DS
authors.lastname: Park
authors.affiliation: Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea.
authors.forename: Kyung-A
authors.initials: KA
authors.lastname: Hwang
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D056747:Immunomodulation / Q000187:drug effects; D007371:Interferon-gamma / Q000096:biosynthesis*; D037602:Isodon / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000276:immunology*; D007938:Leukemia / Q000188:drug therapy; D061986:MCF-7 Cells; D008297:Male; D055670:Medicine, Korean Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D007155:Immunologic Factors; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma