title: [Effects of polychlorinated biphenyl on the expression of c-fos and c-jun in mesenchymal cells of rats].
abstract: OBJECTIVE
  To study the effects of polychlorinated biphenyl (PCB126) on the expression of c-fos and c-jun in mesenchymal cells (MSCs) of rats.
  
  
  METHODS
  MSCs were separated by percoll cell separating medium (density 1.073 g/cm3). After being cultured and transferred, the cells were divided into a control group, a low-dose group (10(-7) mol/L PCB126) and a high-dose group (10(-6) mol/L PCB126). The cellular growth rate and the expression of c-fos and c-jun protein were analyzed by MTT, immunofluorescence chemical assay, RT-PCR and Western blot.
  
  
  RESULTS
  The cellular growth rate in the low-and high-dose group were 13.8% and 19.1% at 12 h, 31.5% and 36.1% at 24 h, 42.5% and 43.6% at 48 h, respectively (P < 0.01). The positive rates of c-fos expression in the low-and high-dose groups were 54.6% and 51.3% at 12 h, and 83.2% and 73.0% at 24 h. The expression of c-fos mRNA detected by RT-PCR was upregulated at 30 min and 1h in PCB126 groups. The expression of c-jun mRNA were much higher in PCB126 groups than that in the control group. The expression of c-fos and c-jun protein was upregulated in the low-and high-dose groups at 12 h and 24 h.
  
  
  CONCLUSIONS
  PCB126 could promote the proliferation of MSCs and upregulate the expression of cancer associated gene c-fos and c-jun. The effect of PCB126 on the function of MSCs might be associated with the abnormal expression of c-fos and c-jun gene.
authors.affiliation: General Hospital of Lanzhou Military Command, Lanzhou 730050, China. zhao6703@yahoo.com.cn
authors.forename: Hongbin
authors.initials: H
authors.lastname: Zhao
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Juzi
authors.initials: J
authors.lastname: Dong
authors.forename: Huanfa
authors.initials: H
authors.lastname: Zhou
authors.forename: Yinshu
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D004785:Environmental Pollutants / Q000633:toxicity; D005260:Female; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004785:Environmental Pollutants; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D011078:Polychlorinated Biphenyls; C023035:3,4,5,3',4'-pentachlorobiphenyl