title: Expression of phosphoinositide-specific phospholipase C enzymes in normal endometrium and in endometriosis.
abstract: OBJECTIVE
  To delineate the panel of expression of phosphoinositide-specific phospholipase C (PI-PLC) signaling enzymes in normal endometrium and in endometriosis.
  
  
  METHODS
  Clinical/experimental study.
  
  
  METHODS
  University.
  
  
  METHODS
  Healthy donor woman and endometriosis-affected woman.
  
  
  METHODS
  Normal endometrium and endometriosis surgical biopsies were analyzed using gene expression analyses methodology (reverse transcriptase-polymerase chain reaction [PCR], bioanalyses).
  
  
  METHODS
  Gene expression (messenger RNA concentration) measures of 12 PI-PLC enzymes: PI-PLC β1, PI-PLC β2, PI-PLC β3, PI-PLC β4, PI-PLC γ1, PI-PLC γ2, PI-PLC δ1, PI-PLC δ3, PI-PLC δ4, PI-PLC ε, PI-PLC η1, and PI-PLC η2.
  
  
  RESULTS
  PI-PLC β1, PI-PLC β3, PI-PLC δ1, and PI-PLC δ3 enzymes were detected, although differently expressed in normal and endometriosis tissues.
  
  
  CONCLUSIONS
  The involvement of PI-PLC enzymes in inflammation and the consistency of susceptible endometriosis loci with PI-PLC genes mapping corroborate the hypothesis that PI signaling might be involved in the pathogenesis of endometriosis.
authors.affiliation: Department of Sensitive Organs, Policlinic Umberto I, Sapienza University, Rome, Italy. ritalovasco@hotmail.it
authors.forename: Vincenza Rita
authors.initials: VR
authors.lastname: Lo Vasco
authors.forename: Martina
authors.initials: M
authors.lastname: Leopizzi
authors.forename: Caterina
authors.initials: C
authors.lastname: Chiappetta
authors.forename: Rita
authors.initials: R
authors.lastname: Businaro
authors.forename: Patrizia
authors.initials: P
authors.lastname: Polonia
authors.forename: Carlo
authors.initials: C
authors.lastname: Della Rocca
authors.forename: Pietro
authors.initials: P
authors.lastname: Litta
mesh_terms: D000328:Adult; D004715:Endometriosis / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D004717:Endometrium / Q000201:enzymology* / Q000473:pathology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D054801:Phosphoinositide Phospholipase C / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D054801:Phosphoinositide Phospholipase C