title: Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens.
abstract: BACKGROUND
  Launaea procumbens (LP) has been used as a food supplement in Pakistan. In this study methanolic crude extract (LPME) of the whole plant and its different fractions; n-hexane (LPHE); ethyl acetate (LPEE) and chloroform (LPCE) were studied for the determination of total flavonoid and phenolics contents along with multifaceted in vitro scavenging assays.
  
  
  RESULTS
  Considerable amount of flavonoid and phenolics contents were found in all the fractions. Methanol and chloroform fraction exhibited efficient scavenging of DPPH·, ABTS·+, ·OH, superoxide, lipid peroxide and nitric oxide free radicals. Significant correlation was found between DPPH·, ABTS·+, superoxide radical, β-carotene bleaching restraint and phosphomolybdenum assay with total flavonoids and phenolics contents. High performance chromatography (HPLC) of LPME revealed the presence of vitexin, orientin, rutin, hyperoside, catechin and myricetin.
  
  
  CONCLUSIONS
  These results reveal the presence of bioactive compounds in LPME, which might be contributed towards the various in vitro scavenging.
authors.affiliation: Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology, Bannu, KPK, 28100, Pakistan. Rahmatgul_81@yahoo.com.
authors.forename: Rahmat Ali
authors.initials: RA
authors.lastname: Khan
authors.forename: Muhammad Rashid
authors.initials: MR
authors.lastname: Khan
authors.forename: Sumaira
authors.initials: S
authors.lastname: Sahreen
authors.forename: Mushtaq
authors.initials: M
authors.lastname: Ahmed