title: In vitro generation of mature dopamine neurons by decreasing and delaying the expression of exogenous Nurr1.
abstract: Neural stem/progenitor cell (NSC/NPC) cultures can be a source of dopamine (DA) neurons for experimental and transplantation purposes. Nurr1, a steroid receptor transcription factor, can overcome the limitations associated with differentiation of cultured NPCs into DA neurons. However, forced Nurr1 expression in NPC cultures generates non-neuronal and/or immature DA cells. We show here that the Nurr1 level and period of expression crucially affect the differentiation and maturation of Nurr1-induced DA neurons. Mature DA neurons were generated by manipulating Nurr1 expression patterns to resemble those in the developing midbrain.
authors.affiliation: Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Korea.
authors.forename: Chang-Hwan
authors.initials: CH
authors.lastname: Park
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Lim
authors.forename: Yong-Hee
authors.initials: YH
authors.lastname: Rhee
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Yi
authors.forename: Boe-Kyoung
authors.initials: BK
authors.lastname: Kim
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Shim
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Sung Jun
authors.initials: SJ
authors.lastname: Jung
authors.forename: Sang-Hun
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism; D004298:Dopamine / Q000096:biosynthesis; D059290:Dopaminergic Neurons / Q000378:metabolism*; D047108:Embryonic Development; D008636:Mesencephalon / Q000254:growth & development* / Q000378:metabolism; D055495:Neurogenesis; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2 / Q000096:biosynthesis*; D051381:Rats
chemical_list: C539884:Nr4a2 protein, rat; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D004298:Dopamine