title: Molecular epidemiology of Mannheimia haemolytica and Mannheimia glucosida associated with ovine mastitis.
abstract: While Mannheimia haemolytica and Mannheimia glucosida have been recognized as causes of intramammary infection in sheep, there has been no investigation of the epidemiology of the strains involved. Pulsed field gel electrophoresis was used to study the molecular epidemiology of isolates of these 2 species associated with ovine mastitis. Ten distinct strains were recognized among 12 M. haemolytica isolates, and 7 distinct strains among 13 M. glucosida isolates. The results demonstrate a high diversity of isolates with the ability to cause ovine mastitis. However, the presence of some identical isolates may suggest the possibility of horizontal transmission of these species in some flocks, possibly through lamb sucking, and/or differences in the capacity of some isolates to cause mastitis in sheep.
authors.affiliation: Asia-Pacific Centre for Animal Health and School of Veterinary Science, School of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Lida
authors.initials: L
authors.lastname: Omaleki
authors.forename: Glenn F
authors.initials: GF
authors.lastname: Browning
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Allen
authors.forename: Stuart R
authors.initials: SR
authors.lastname: Barber
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005260:Female; D008297:Male; D031121:Mannheimia / Q000235:genetics / Q000302:isolation & purification*; D016978:Mannheimia haemolytica / Q000235:genetics / Q000302:isolation & purification*; D008413:Mastitis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D017720:Molecular Epidemiology; D016871:Pasteurellaceae Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial