title: The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells.
abstract: Sodium hyaluronate (HA) was associated with dopamine (DPA) and introduced as a coating for maghemite (γ-Fe(2)O(3)) nanoparticles obtained by the coprecipitation of iron(II) and iron(III) chlorides and oxidation with sodium hypochlorite. The effects of the DPA anchorage of HA on the γ-Fe(2)O(3) surface on the physicochemical properties of the resulting colloids were investigated. Nanoparticles coated at three different DPA-HA/γ-Fe(2)O(3) and DPA/HA ratios were chosen for experiments with rat bone marrow mesenchymal stem cells and human chondrocytes. The nanoparticles were internalized into rat bone marrow mesenchymal stem cells via endocytosis as confirmed by Prussian Blue staining. The efficiency of mesenchymal stem cell labeling was analyzed. From among the investigated samples, efficient cell labeling was achieved by using DPA-HA-γ-Fe(2)O(3) nanoparticles with DPA-HA/γ-Fe(2)O(3) = 0.45 (weight/ weight) and DPA/HA = 0.038 (weight/weight) ratios. The particles were used as a contrast agent in magnetic resonance imaging for the labeling and visualization of cells.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, Prague, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Babic
authors.forename: Daniel
authors.initials: D
authors.lastname: Horak
authors.forename: Pavla
authors.initials: P
authors.lastname: Jendelova
authors.forename: Vit
authors.initials: V
authors.lastname: Herynek
authors.forename: Vladimir
authors.initials: V
authors.lastname: Proks
authors.forename: Vaclav
authors.initials: V
authors.lastname: Vanecek
authors.forename: Petr
authors.initials: P
authors.lastname: Lesny
authors.forename: Eva
authors.initials: E
authors.lastname: Sykova
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003287:Contrast Media; D004298:Dopamine / Q000737:chemistry*; D004705:Endocytosis; D005295:Ferrocyanides; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D050997:Nanomedicine; D010316:Particle Size; D051381:Rats
chemical_list: D020099:Coated Materials, Biocompatible; D003287:Contrast Media; D005295:Ferrocyanides; D058185:Magnetite Nanoparticles; D006820:Hyaluronic Acid; C000170:ferric ferrocyanide; D004298:Dopamine