title: Functional MMP-9 polymorphisms modulate plasma MMP-9 levels in multiple sclerosis patients.
abstract: We have described that MMP-9 C(-1562)T and (CA)(n) polymorphisms contribute to multiple sclerosis (MS). Here, we evaluate whether plasma MMP-9 levels are related to disease severity, drug therapy resistance and polymorphisms. For sub-study 1, 36 patients with MS and 35 controls were recruited. For sub-study 2, 88 individuals (53 patients and 35 controls) were included in a cross-sectional analysis. MS patients presented higher MMP-9 activity (1.4±0.18 versus 0.93±0.18A.U. for control, P<0.05). Drug-therapy resistant individuals exhibited increased MMP-9 activity (1.96±0.25 versus 1.21±0.09A.U. for non-resistant patients). EDSS score was also related to MMP-9 levels. The CT+TT and HH genotypes had higher MMP-9 levels as compared to patients carrying the CC and LL. Drug therapy resistance, disease severity, MMP-9 plasma activity and polymorphisms are associated with MS.
authors.affiliation: Department of Pharmacology, Medical School of Ribeirão Preto, University of São Paulo, USP, Av. Bandeirantes 3900, Ribeirão Preto, SP, Brazil.
authors.forename: Karla Simone da Silva
authors.initials: KS
authors.lastname: Fernandes
authors.forename: Doralina Guimaraes
authors.initials: DG
authors.lastname: Brum
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Palei
authors.forename: Valeria Cristina
authors.initials: VC
authors.lastname: Sandrim
authors.forename: Carlos Tostes
authors.initials: CT
authors.lastname: Guerreiro
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Tanus-Santos
authors.forename: Amilton Antunes
authors.initials: AA
authors.lastname: Barreira
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D004351:Drug Resistance / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood* / Q000235:genetics*; D009103:Multiple Sclerosis / Q000097:blood* / Q000235:genetics*; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020780:Matrix Metalloproteinase 9