title: Clara cell protein expression in human neonates during respiratory distress syndrome.
abstract: OBJECTIVE
  Clara cell protein (cc-10) has been shown to negatively regulate inflammation, protect pulmonary surfactant from degradation in the lung, and administration of this recombinant protein improves the condition of infant respiratory distress syndrome (iRDS), a disease that occurred mainly in preterm infants. In view of the possibility that altered expression of cc-10 might regulate its protective function, we attempted to characterize this protein in infants with iRDS.
  
  
  METHODS
  Using bronchotraqueal aspirates from human infants, we analyzed cc-10 in two-dimensional gel electrophoresis (2-DE) by combining immunoprecipitation, carbonyl groups and total protein immunoblotting.
  
  
  RESULTS
  Seven forms of cc-10 were detected with western immunoblots in infants with iRDS while only four forms were present in neonates who needed mechanical ventilation for other reasons without any lung disease (control group). The overall levels of cc-10 in iRDS were lower and differences were seen in isoform pattern and distribution.
  
  
  CONCLUSIONS
  Our demonstration that cc-10 is differentially expressed in infants with iRDS may point the way towards one possible mechanism that potentially involves modifications of the protein structure with its anti-inflammatory and surfactant protective function and could be detrimental for this airway disorder.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Instituto Nacional de Perinatología IER Mexico City, Mexico.
authors.forename: Joel
authors.initials: J
authors.lastname: Arias-Martínez
authors.forename: Miguel
authors.initials: M
authors.lastname: Palacios-Sánchez
authors.forename: Dagoberto
authors.initials: D
authors.lastname: Delgado-Franco
authors.forename: José
authors.initials: J
authors.lastname: Guzmán-Bárcenas
authors.forename: Ethel
authors.initials: E
authors.lastname: García-Latorre
authors.forename: Limei
authors.initials: L
authors.lastname: Zhang
authors.forename: Claudine
authors.initials: C
authors.lastname: Irles
mesh_terms: D015153:Blotting, Western; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D020033:Protein Isoforms / Q000378:metabolism*; D012127:Respiratory Distress Syndrome, Newborn / Q000378:metabolism / Q000473:pathology*; D014598:Uteroglobin / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; C556389:SCGB1A1 protein, human; D014598:Uteroglobin