title: Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics.
abstract: Traditional white-light and fluorescent imaging techniques provide powerful methods to extract high-resolution information from two-dimensional (2-D) sections, but to retrieve information from a three-dimensional (3-D) volume they require relatively slow scanning methods that result in increased acquisition time. Using an ultra-high speed liquid lens, we circumvent this problem by simultaneously acquiring images from multiple focal planes. We demonstrate this method by imaging microparticles and cells flowing in 3-D microfluidic channels.
authors.forename: Martí
authors.initials: M
authors.lastname: Duocastella
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Craig B
authors.initials: CB
authors.lastname: Arnold
mesh_terms: D000162:Acoustics / Q000295:instrumentation*; D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005246:Feedback; D007089:Image Enhancement / Q000295:instrumentation*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D007909:Lenses; D008029:Lighting / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity