title: The influence of genetic factors on the osteoinductive potential of calcium phosphate ceramics in mice.
abstract: The efficacy of calcium phosphate (CaP) ceramics in healing large bone defects is, in general, not as high as that of autologous bone grafting. Recently, we reported that CaP ceramics with osteoinductive properties were as efficient in healing an ilium defect of a sheep as autologous bone graft was, which makes this subclass of CaP ceramics a powerful alternative for bone regeneration. Although osteoinduction by CaP ceramics has been shown in several large animal models it is sporadically reported in mice. Because the lack of a robust mouse model has delayed understanding of the mechanism, we screened mice from 11 different inbred mouse strains for their responsiveness to subcutaneous implantation of osteoinductive tricalcium phosphate (TCP). In only two strains (FVB and 129S2) the ceramic induced bone formation, and in particularly, in FVB mice, bone was found in all the tested mice. We also demonstrated that other CaP ceramics induced bone formation at the same magnitude as that observed in other animal models. Furthermore, VEGF did not significantly increase TCP induced bone formation. The mouse model here described can accelerate research of osteoinductive mechanisms triggered by CaP ceramics and potentially the development of therapies for bone regeneration.
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Drienerlolaan 5, 7522 NB Enschede, The Netherlands.
authors.forename: Ana M C
authors.initials: AM
authors.lastname: Barradas
authors.forename: Huipin
authors.initials: H
authors.lastname: Yuan
authors.forename: Johan
authors.initials: J
authors.lastname: van der Stok
authors.forename: Bach
authors.initials: B
authors.lastname: Le Quang
authors.forename: Hugo
authors.initials: H
authors.lastname: Fernandes
authors.forename: Anindita
authors.initials: A
authors.lastname: Chaterjea
authors.forename: Marieke C H
authors.initials: MC
authors.lastname: Hogenes
authors.forename: Kathy
authors.initials: K
authors.lastname: Shultz
authors.forename: Leah Rae
authors.initials: LR
authors.lastname: Donahue
authors.forename: Clemens
authors.initials: C
authors.lastname: van Blitterswijk
authors.forename: Jan
authors.initials: J
authors.lastname: de Boer
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology*; D002460:Cell Line; D002516:Ceramics / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D010012:Osteogenesis / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; C018392:tricalcium phosphate