title: Isolation and characterization of a novel filamentous phage from Stenotrophomonas maltophilia.
abstract: In this study, a novel filamentous phage, φSHP1, of the environmental Stenotrophomonas maltophilia strain P2 was isolated and characterized. Electron microscopy showed that φSHP1 resembled members of the family Inoviridae and was about 2.1 μm long. The 6,867-nucleotide genome of φSHP1 was a circular single-stranded DNA and had a replication form designated pSH1. Ten putative open reading frames (ORFs) were found in the φSHP1 genome, and six predicted proteins showed similarity to proteins in databases. Tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis of φSHP1 displayed one major structural polypeptide of approximately 4.0 kDa. N-terminal sequencing showed that it was the mature product of ORF5 and that its N-terminal 27 amino acid residues had been cleaved off from the predicted nascent protein. Finally, phylogenetic trees were constructed to analyze the phylogenetic relationship of φSHP1 to other known filamentous phages. φSHP1 appears to be the first reported Stenotrophomonas filamentous phage.
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Shen
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Huang
mesh_terms: D016000:Cluster Analysis; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D017911:Inoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D017912:Inovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D020615:Stenotrophomonas maltophilia / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D014764:Viral Proteins