title: Revisit of imidazole-zinc reverse stain for protein polyacrylamide gel electrophoresis.
abstract: Imidazole-zinc reverse stain (ZN stain) is known for its high sensitivity, ease of use, and cost-effective feature. ZN stain is compatible to many experiments of which those are proteomics-related in particular. Here, we describe the ZN staining procedures and the subsequent procedures incorporated in detail, along with the improvements of setup in aspects of visualization and documentation for post-processing ZN-stained gel images.
authors.affiliation: Catholic Fu-Jen University, New Taipei City, Taiwan. 056489@mail.fju.edu.tw
authors.forename: Han-Min
authors.initials: HM
authors.lastname: Chen
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D002021:Buffers; D045744:Cell Line, Tumor; D002712:Chlorides / Q000737:chemistry*; D004396:Coloring Agents; D005404:Fixatives / Q000737:chemistry; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013194:Staining and Labeling / Q000379:methods*; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D002021:Buffers; D002712:Chlorides; D004396:Coloring Agents; D005404:Fixatives; D007093:Imidazoles; D011506:Proteins; D017967:Zinc Compounds; C029899:imidazole; C016837:zinc chloride; D019342:Acetic Acid