title: Kidney injury and cell therapy: Preclinical study.
abstract: The aim of this study is to show histological and immunofluorescence analysis of renal parenchyma of agoutis affected by gentamicin-induced renal disease after the infusion of bone marrow mononuclear cells (BMMC) stained with Hoechst®. Nine agouti's males were divided into three groups: Test group (TG): renal disease by gentamicin induced (n = 3), cell therapy group (CTG): renal disease by gentamicin induced and BMMC infusion (n = 3), and control group (CG): nonrenal disease and BMMC infusion (n = 3). TG and CTG were submitted to the protocol of renal disease induction using weekly application of gentamicin sulfate for 4 months. CG and CTG received a 1 × 108 BMMC stained with Hoechst and were euthanized for kidney examination 21 days after BMMC injection and samples were collected for histology and immunofluorescence analysis. Histological analysis demonstrated typical interstitial lesions in kidney similarly to human disease, as tubular necrosis, glomerular destruction, atrophy tubular, fibrotic areas, and collagen deposition. We conclude that histological analysis suggest a positive application of agouti's as a model for a gentamicin inducing of kidney disease, beyond the immunofluorescence analysis suggest a significant migration of BMMC to sites of renal injury in CTG.
authors.affiliation: Department of Veterinary Clinical and Surgery, Federal University of Piauí (UFPI), Teresina, Piauí, Brazil. rosacabral@ufpi.edu
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Cabral
authors.forename: Maíra S
authors.initials: MS
authors.lastname: Ferraz
authors.forename: Márcia  dos S
authors.initials: M
authors.lastname: Rizzo
authors.forename: Francisco Das Chagas A
authors.initials: FD
authors.lastname: Sousa
authors.forename: Nirnheila M
authors.initials: NM
authors.lastname: Rodrigues
authors.forename: Pollyanna B
authors.initials: PB
authors.lastname: Ibiapina
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Ambrósio
authors.forename: Maria Acelina M
authors.initials: MA
authors.lastname: De Carvalho
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D004195:Disease Models, Animal; D005839:Gentamicins / Q000009:adverse effects*; D006651:Histocytochemistry; D007668:Kidney / Q000473:pathology; D008297:Male; D008856:Microscopy, Fluorescence; D051437:Renal Insufficiency / Q000139:chemically induced* / Q000628:therapy*; D012377:Rodentia
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins