title: Phylogenetic analysis of Bolivian bat trypanosomes of the subgenus schizotrypanum based on cytochrome B sequence and minicircle analyses.
abstract: The aim of this study was to establish the phylogenetic relationships of trypanosomes present in blood samples of Bolivian Carollia bats. Eighteen cloned stocks were isolated from 115 bats belonging to Carollia perspicillata (Phyllostomidae) from three Amazonian areas of the Chapare Province of Bolivia and studied by xenodiagnosis using the vectors Rhodnius robustus and Triatoma infestans (Trypanosoma cruzi marenkellei) or haemoculture (Trypanosoma dionisii). The PCR DNA amplified was analyzed by nucleotide sequences of maxicircles encoding cytochrome b and by means of the molecular size of hyper variable regions of minicircles. Ten samples were classified as Trypanosoma cruzi marinkellei and 8 samples as Trypanosoma dionisii. The two species have a different molecular size profile with respect to the amplified regions of minicircles and also with respect to Trypanosoma cruzi and Trypanosoma rangeli used for comparative purpose. We conclude the presence of two species of bat trypanosomes in these samples, which can clearly be identified by the methods used in this study. The presence of these trypanosomes in Amazonian bats is discussed.
authors.affiliation: Instituto de Investigaciones Biomédicas IIBISMED, Facultad de Medicina, Universidad Mayor de San Simón, Cochabamba, Bolivia.
authors.forename: Lineth
authors.initials: L
authors.lastname: García
authors.forename: Sylvia
authors.initials: S
authors.lastname: Ortiz
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Osorio
authors.forename: Mary Cruz
authors.initials: MC
authors.lastname: Torrico
authors.forename: Faustino
authors.initials: F
authors.lastname: Torrico
authors.forename: Aldo
authors.initials: A
authors.lastname: Solari
mesh_terms: D000818:Animals; D001838:Bolivia; D002685:Chiroptera / Q000469:parasitology*; D045303:Cytochromes b / Q000235:genetics*; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014345:Trypanosoma / Q000235:genetics*
chemical_list: D015800:Protozoan Proteins; D045303:Cytochromes b