title: Inhibition of lysenin-induced hemolysis by all-E-lutein derived from the plant Dalbergia latifolia.
abstract: Lysenin is a pore-forming toxin derived from coelomic fluid of the earthworm Eisenia foetida. The model of lysenin-induced hemolysis includes the specific binding of lysenin to sphingomyelin, oligomerization of the pore proteins, and pore formation. Although the mechanism of lysenin-induced hemolysis is unique, its precise mechanism of action and its inhibitors are poorly understood. In the present study, we screened for inhibitors of lysenin-induced hemolysis by using an optimized screening system and found a methanolic extract of Dalbergia latifolia leaves to be a potential candidate. After isolation and identification, all-E-lutein was identified as the hemolysis inhibitor with an effective dose of 0.025-2.5 ng/mL without any toxicity. The inhibition by all-E-lutein is likely to occur during the receptor binding and/or pore-forming protein oligomerization.
authors.affiliation: Faculty of Science and Technology, Keio University, Kanagawa, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Niwa
authors.forename: Chino
authors.initials: C
authors.lastname: Matsui
authors.forename: Neelanun
authors.initials: N
authors.lastname: Sukumwang
authors.forename: Hironobu
authors.initials: H
authors.lastname: Iinuma
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Takashi
authors.initials: T
authors.lastname: Koyano
authors.forename: Taworn
authors.initials: T
authors.lastname: Kovitayakorn
authors.forename: Siro
authors.initials: S
authors.lastname: Simizu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Umezawa
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D035782:Dalbergia / Q000737:chemistry*; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects*; D006461:Hemolysis; D014975:Lutein / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011485:Protein Binding; D012756:Sheep; D013109:Sphingomyelins / Q000737:chemistry; D014118:Toxins, Biological / Q000009:adverse effects / Q000037:antagonists & inhibitors*
chemical_list: D010936:Plant Extracts; D013109:Sphingomyelins; D014118:Toxins, Biological; C107040:lysenin; D014975:Lutein; D000432:Methanol