title: Predicting protein dynamic binding capacity from batch adsorption tests.
abstract: The dynamic binding capacity (DBC) and its dependence on residence time influence the design and productivity of adsorption columns used in protein capture applications. This paper offers a very simple approach to predict the DBC of an adsorption column based on a measurement of the equilibrium binding capacity (EBC) and of the time needed to achieve one-half of the EBC in a batch adsorption test. The approach is based on a mass transfer kinetics model that assumes pore diffusion with a rectangular isotherm; however, the same approach is also shown to work for other systems where solute transport inside the particle occurs through other transport mechanisms.
authors.affiliation: Department of Chemical Engineering, University of Virginia, Charlottesville, VA 22904, USA. gc@virginia.edu
authors.forename: Giorgio
authors.initials: G
authors.lastname: Carta
mesh_terms: D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D007700:Kinetics; D008956:Models, Chemical; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011506:Proteins