title: Wnt-4 potently inhibits capillary outgrowth from rat aorta in 3D culture.
abstract: Regulation of angiogenesis involves tight cell-to-cell and cell-to-extracellular-matrix interactions. Various reports demonstrate that the Wnt signaling pathways participate in this regulation. Using a three-dimensional aortic ring culture combined with an ex vivo retroviral infection approach, we evaluated the effects of two Wnt growth factors, Wnt-1 and Wnt-4, on the formation and growth of new capillaries. Our results show that Wnt-1 had no effect, whereas Wnt-4 was a potent inhibitor of capillary outgrowth in vitro.
authors.affiliation: Groupe Rein & Hypertension, Institut Universitaire de Recherche Clinique, CNRS FRE3400, 641 Ave Doyen Giraud, 34093, Montpellier Cedex 5, France. bernard.jover@inserm.fr
authors.forename: Bernard
authors.initials: B
authors.lastname: Jover
authors.forename: Daphné
authors.initials: D
authors.lastname: Girardot
authors.forename: Françoise
authors.initials: F
authors.lastname: de Courtois Roy de Vacquières
authors.forename: Daniel
authors.initials: D
authors.lastname: Casellas
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Molès
mesh_terms: D016475:3T3 Cells; D020533:Angiogenesis Inhibitors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001012:Aorta, Abdominal / Q000166:cytology / Q000378:metabolism*; D002196:Capillaries / Q000166:cytology / Q000378:metabolism*; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000378:metabolism; D046509:Tissue Culture Techniques; D051155:Wnt1 Protein / Q000235:genetics / Q000378:metabolism; D060528:Wnt4 Protein / Q000235:genetics / Q000378:metabolism*; D000099274:Frizzled-Related Proteins
chemical_list: D020533:Angiogenesis Inhibitors; D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D051155:Wnt1 Protein; D060528:Wnt4 Protein; D000099274:Frizzled-Related Proteins; C495246:Wnt1 protein, mouse; C555582:Wnt4 protein, mouse