title: Elemental micro-imaging and quantification of human substantia nigra using synchrotron radiation based x-ray fluorescence--in relation to Parkinson's disease.
abstract: Synchrotron radiation based x-ray fluorescence (SRXRF) was applied to the quantitative evaluation of elemental changes in substantia nigra pars compacta (SNc) in Parkinson's disease (PD) in the framework of a study on the role of chemical elements in the pathophysiology of PD. The analysis was carried out for dopaminergic nerve cells and extraneuronal spaces. The mass fractions of P, S, Cl, K, Ca, Fe, Cu, Zn, Br and Rb were determined. The application of standard samples developed especially for the determination of elemental mass fractions in thin tissue sections using the SRXRF technique is presented. Two-dimensional maps of elemental distribution show that the location of nerve cells in SNc sections is precisely visualized by the high levels of most elements. It was found that statistically significant differences between control and PD neurons are observed for S (p = 0.04), Cl (p = 0.02), Ca (p = 0.08), Fe (p = 0.04) and Zn (p = 0.04). The mass fractions of P (p = 0.08), S (p = 0.07), Cl (p = 0.04), Zn (p = 0.08) and Rb (p = 0.08) in areas outside the nerve cell bodies differed significantly between PD and control groups. A clear cluster separation between the PD nerve cells and neurons representing the control group was noticed. It was found that Cl, Fe, Ca and Zn are the most significant elements in the general discrimination between PD nerve cells and the control. The comparison between the extraneuronal spaces showed that Cl, Fe and Cu differentiate the PD and control group the most. The evident contribution of chemical elements to the pathophysiology of PD was shown.
authors.affiliation: AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Krakow, Poland. Magdalena.Boruchowska@fis.agh.edu.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Szczerbowska-Boruchowska
authors.forename: Anna
authors.initials: A
authors.lastname: Krygowska-Wajs
authors.forename: Dariusz
authors.initials: D
authors.lastname: Adamek
mesh_terms: D006801:Humans; D057054:Molecular Imaging / Q000295:instrumentation*; D010300:Parkinson Disease / Q000378:metabolism* / Q000473:pathology; D013052:Spectrometry, X-Ray Emission / Q000295:instrumentation*; D013378:Substantia Nigra / Q000378:metabolism* / Q000473:pathology; D017356:Synchrotrons