title: Transcription and induction profiles of three novel P450 genes in Liposcelis bostrychophila (Psocoptera: Liposcelididae).
abstract: The cDNAs of three novel P450 genes, CYP4CB1, CYP4CC1, and CYP4CD1 (GenBank accessions EU979550, EU979549, and EU979551, respectively), were sequenced and characterized from the psocid Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae). CYP4CB1, CYP4CC1, and CYP4CD1 contain open reading frames of 1533, 1512, and 1536 nucleotides that encode 511,504, and 512 amino acid residues, respectively. The putative proteins of CYP4CB1, CYP4CC1, and CYP4CD1 show predicted molecular mass of 59.65, 58.87, and 59.71 kDa, with a theoretical isoelectric point of 6.59, 8.80, and 8.84, respectively. The N-terminal transmembrane domain was only found in CYP4CB1 suggested it is a typical microsomal P450. Phylogenetic analysis showed a close relationship of CYP4CB1, CYP4CC1, and CYP4CD1 with CYP4AW1, CYP4L4, and CYP4E2. Quantitative real-time reverse transcriptase-polymerase chain reaction indicated that these three genes were expressed at all tested developmental stages. In addition, the highest expression occurred in the adult stage, which suggested that these three P450 genes may play important roles in adulthood. The transcripts of CYP4CB1 and CYP4CC1 in adult psocids could be induced to the highest level at 36 and 24 h after the exposure to deltamethrin and paraoxon-methyl (50% lethal concentration [LC50]), respectively, whereas CYP4CD1 remained unchanged. CYP4CD1 transcripts, however, increased rapidly at 8 h after aldicarb (LC50) induction and reached the peak at 36 h. The induction profiles of the three P450 genes suggested that CYP4CB1 and CYP4CC1 are possibly associated with deltamethrin and paraoxonmethyl metabolism in psocids, whereas CYP4CD1 is probably involved in aldicarb metabolism. However, our assumption needs to be further verified by recombinant protein expression of these proteins as well as RNA interference of these genes.
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, People's Republic of China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Dou
authors.forename: Pei-An
authors.initials: PA
authors.lastname: Tang
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000448:Aldicarb / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics*; D007313:Insecta / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D009570:Nitriles / Q000494:pharmacology; D009758:Nymph / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D010261:Paraoxon / Q000031:analogs & derivatives / Q000494:pharmacology; D010802:Phylogeny; D011722:Pyrethrins / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D000448:Aldicarb; D003577:Cytochrome P-450 Enzyme System; D010261:Paraoxon; C008480:methylparaoxon