title: Analytical evaluation of Sysmex UF-1000i for flow cytometric analysis of peritoneal fluid.
abstract: OBJECTIVE
  To evaluate analytical performance of Sysmex UF-1000i for peritoneal fluid analysis.
  
  
  METHODS
  Functional sensitivity, imprecision, linearity and comparison studies were performed on peritoneal fluids.
  
  
  RESULTS
  Total imprecision was 1.6-4.7%, functional sensitivity 27/μL for white blood cell (WBC) and 32/μL for total nucleated cell (TNC) count. Linearity was excellent up to 983 cell/μL, carry-over <0.2%, correlation with manual microscopy always greater than 0.992.
  
  
  CONCLUSIONS
  The instrument exhibited optimal performance at the conventional WBC diagnostic thresholds.
authors.affiliation: Laboratorio Analisi Chimico Cliniche, AO Ospedali Riuniti Bergamo, Bergamo, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Buoro
authors.forename: Rosanna
authors.initials: R
authors.lastname: Gustinetti
authors.forename: Paola
authors.initials: P
authors.lastname: Dominoni
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Alessio
authors.forename: Patrizia
authors.initials: P
authors.lastname: Filisetti
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Lucà
authors.forename: Stefano
authors.initials: S
authors.lastname: Fagiuoli
authors.forename: Cosimo
authors.initials: C
authors.lastname: Ottomano
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Lippi
mesh_terms: D001202:Ascitic Fluid / Q000166:cytology*; D002452:Cell Count; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity