title: Evaluation of E6 and E7 mRNA expression in HPV DNA positive breast cancer.
abstract: Several studies have suggested a possible role for HPV in the pathogenesis of the breast cancer. We investigated the presence of the HPV DNA in breast cancers and non malignant disease breast tissues by the use of a standard HPV detection method (INNO-Lipa HPV), in order to detect HPV DNA in metastatic nodes, to investigate a possible cervical HPV co-infection, and to evaluate the E6/E7 mRNA expression in HPV DNA positive breast cancer tissues. The rate of HPV infection was significantly higher in the cancer group than in controls (9/31 vs. 0/12, p = 0.04). One out of eight metastatic axillary nodes was positive for HPV infection; 2/3 of the positive HPV breast cancer patients were co-infected at the cervical site. The role of the virus in breast oncogenesis is still unclear, since our analysis failed in demonstrating the expression of viral E6 and E7 in positive HPV positive breast tumor tissues.
authors.affiliation: Department of Woman's Health and Territorial Medicine, Sant' Andrea Hospital, Faculty of Medicine and Psychology, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Frega
authors.forename: L
authors.initials: L
authors.lastname: Lorenzon
authors.forename: M
authors.initials: M
authors.lastname: Bononi
authors.forename: A
authors.initials: A
authors.lastname: De Cesare
authors.forename: A
authors.initials: A
authors.lastname: Ciardi
authors.forename: D
authors.initials: D
authors.lastname: Lombardi
authors.forename: C
authors.initials: C
authors.lastname: Assorgi
authors.forename: M
authors.initials: M
authors.lastname: Gentile
authors.forename: M
authors.initials: M
authors.lastname: Moscarini
authors.forename: M R
authors.initials: MR
authors.lastname: Torrisi
authors.forename: D
authors.initials: D
authors.lastname: French
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism* / Q000821:virology; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000821:virology; D018275:Carcinoma, Lobular / Q000378:metabolism* / Q000821:virology; D004279:DNA, Viral / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D018226:Fibroadenoma / Q000378:metabolism* / Q000821:virology; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000302:isolation & purification; D058952:Human papillomavirus 31 / Q000302:isolation & purification; D052163:Human papillomavirus 6 / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000378:metabolism; D010212:Papilloma / Q000378:metabolism* / Q000821:virology; D050725:Papillomavirus E7 Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C068651:E6 protein, Human papillomavirus type 16; C052603:E6 protein, Human papillomavirus type 18; C572886:E6 protein, Human papillomavirus type 31; C052633:E7 protein, Human papillomavirus type 18; C502189:E7 protein, Human papillomavirus type 31; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16