title: [The construction and expression of Saccharomyces albicans pACT1-GFP plasmids].
abstract: OBJECTIVE
  To construct strains containing green fluorescent protein (GFP) to study gene regulation in Saccharomyces albicans cells during the infection process.
  
  
  METHODS
  pACT1-GFP was constructed, and Saccharomyces albicans CAI4 was transformed. The expression of GFP in yeast and hyphal compartments was observed with microscopy.
  
  
  RESULTS
  99% of Saccharomyces albicans cells containing pACT1-GFP fusion displayed significant fluorescence levels both in the yeast and hyphal compartments. The fluorescence intensity in two compartments had no obvious difference.
  
  
  CONCLUSIONS
  pACT1-GFP can be expressed stably in the yeast cells.
authors.affiliation: School of Stomatology of Shandong University, Key Laboratory of Oral Biomedicine of Shandong Province, Jinan 250012, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Fen
authors.initials: F
authors.lastname: Jia
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Xia
authors.forename: Hua
authors.initials: H
authors.lastname: Qian
authors.forename: Hongnan
authors.initials: H
authors.lastname: Dong
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Qi
mesh_terms: D002176:Candida albicans; D049452:Green Fluorescent Proteins; D006801:Humans; D010957:Plasmids; D012440:Saccharomyces; D012441:Saccharomyces cerevisiae
chemical_list: D049452:Green Fluorescent Proteins