title: Improvement of hydrogen productivity by introduction of NADH regeneration pathway in Clostridium paraputrificum.
abstract: To improve the hydrogen productivity and examine the hydrogen evolution mechanism of Clostridium paraputrificum, roles of formate in hydrogen evolution and effects of introducing formate-originated NADH regeneration were explored. The formate-decomposing pathway for hydrogen production was verified to exist in C. paraputrificum. Then NAD(+)-dependent formate dehydrogenase FDH1 gene (fdh1) from Candida boidinii was overexpressed, which regenerate more NADH from formate to form hydrogen by NADH-mediated pathway. With fdh1 overexpression, the hydrogen yield via NADH-involving pathway increased by at least 59 % compared with the control. Accompanied by the change of hydrogen metabolism, the whole cellular metabolism was redistributed greatly.
authors.affiliation: Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.forename: Hongxin
authors.initials: H
authors.lastname: Zhao
authors.forename: Xin-Hui
authors.initials: XH
authors.lastname: Xing
mesh_terms: D000693:Anaerobiosis; D002175:Candida / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D003013:Clostridium / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D046508:Culture Techniques; D005560:Formate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods*; D006859:Hydrogen / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D009243:NAD / Q000378:metabolism*
chemical_list: D005561:Formates; D009243:NAD; C030544:formic acid; D006859:Hydrogen; D005560:Formate Dehydrogenases