title: Optimization of horseradish peroxidase-catalyzed enhanced chemiluminescence reaction by full factorial design.
abstract: Using a full factorial design the optimization of experimental conditions of enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP-C) in the presence of 3-(10'-phenothiazinyl)propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORP) as enhancers was performed. The effect of concentrations of SPTZ, hydrogen peroxide, MORP, luminol, and Tris on a ratio of peroxidase-catalyzed CL to background was studied. The use of the full 2(5) factorial design instead of "one-variable-a time" method allowed to increase the sensitivity of HRP-C determination 2355 fold without a change of detection limit. The obtained results open up very promising perspectives for using HRP-C-catalyzed ECR to improve the sensitivity of chemiluminescent enzyme immunoassay.
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.
authors.forename: Marina M
authors.initials: MM
authors.lastname: Vdovenko
authors.forename: Alexandra S
authors.initials: AS
authors.lastname: Demiyanova
authors.forename: Tamara A
authors.initials: TA
authors.lastname: Chemleva
authors.forename: Ivan Yu
authors.initials: IY
authors.lastname: Sakharov
mesh_terms: D000465:Algorithms; D017738:Alkanesulfonic Acids / Q000737:chemistry; D002384:Catalysis; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents / Q000737:chemistry*; D007700:Kinetics; D049449:Luminescence; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D009025:Morpholines / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D013843:Thiazines / Q000737:chemistry
chemical_list: C543492:3-(10'-Phenothiazinyl)propane-1-sulfonate; D017738:Alkanesulfonic Acids; D007202:Indicators and Reagents; D009025:Morpholines; D011725:Pyridines; D013843:Thiazines; D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase