title: Isolation of an extremely boron-tolerant strain of  Bacillus firmus.
abstract: A strain of Bacillus firmus (designated strain KC) isolated from a boron (B) mine in California exhibited extreme tolerance to B, provided it was first acclimated at intermediate B supply concentrations. Strain KC tolerated up to 1000 mmol/L B (boric acid-B) and 1800 mmol/L B (sodium tetraborate-B), and attained the greatest growth (as measured by absorbance) at 300 mmol/L B. Despite its extreme tolerance to high B, there was no evidence that it was able to remove significant quantities of B from the growth media, suggesting that strain KC is not likely to be useful for the removal of B from wastewaters in an engineered bioreactor.
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, 111 Koshland Hall, Berkeley, CA 94720-3102, USA.
authors.forename: Matthew F
authors.initials: MF
authors.lastname: Verce
authors.forename: Amanda R
authors.initials: AR
authors.lastname: Stiles
authors.forename: Karen C
authors.initials: KC
authors.lastname: Chong
authors.forename: Norman
authors.initials: N
authors.lastname: Terry
mesh_terms: D000222:Adaptation, Physiological; D001407:Bacillus / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D001483:Base Sequence; D001881:Borates / Q000633:toxicity; D001888:Boric Acids / Q000633:toxicity; D001895:Boron / Q000633:toxicity*; D002140:California; D015386:Hazardous Substances / Q000633:toxicity*; D008969:Molecular Sequence Data
chemical_list: D001881:Borates; D001888:Boric Acids; D015386:Hazardous Substances; C010634:sodium borate; D001895:Boron; C032688:boric acid