title: [Expression of Toll-like receptor 4 in brain tissue of chronic Toxoplasma gondii infection rats and its effect on brain injury].
abstract: OBJECTIVE
  To explore the expression of Toll-like receptor 4 (TLR4) in brain tissue of chronic Toxoplasma infection rats and its effect on brain injury.
  
  
  METHODS
  Ten male SD rats were randomly divided into 2 groups, namely control and infection groups. Each rat in the infection group was intraperitoneal injected with Toxoplasma gondii tachyzoites 10(7)/ml x 2 ml, and that in the control group was injected with 2 ml sterile normal sodium. After 10 weeks, the expression of TLR4 mRNA in the brain was determined by RT-PCR, and the levels of IL-1beta and IL-4 in peripheral blood sera were detected by ELISA.
  
  
  RESULTS
  Compared with the control group, the expression of TLR4 gene and the peripheral blood serum level of IL-1beta of rats in the Toxoplasma gondii infection group were both significantly increased, with all P values were less than 0.05, and the level of IL-4 was also increased, but the difference had no statistically significance (P > 0.05).
  
  
  CONCLUSIONS
  TLR4 might be involved in inflammatory reactions of brain injury for chronic Toxoplasma gondii infection rats.
authors.affiliation: Jiangsu Institute of Parasitic Diseases, Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Wuxi 214064, China.
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Zhou
authors.forename: Shang-Shang
authors.initials: SS
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Tao
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Xu
authors.forename: Yu-Zheng
authors.initials: YZ
authors.lastname: Huang
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology*; D001930:Brain Injuries / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D053583:Interleukin-1beta / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000378:metabolism*; D014122:Toxoplasma / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology
chemical_list: D053583:Interleukin-1beta; D012333:RNA, Messenger; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D015847:Interleukin-4