title: Complete genome sequence of narcissus late season yellows virus infecting Chinese narcissus in China.
abstract: The complete genome sequence of a Chinese narcissus isolate of narcissus late season yellows virus from Zhangzhou, China (NLSYV-ZZ), was determined to be 9,651 nucleotides in length, excluding the 3'-terminal poly (A) tail, by amplification and sequencing of virus RNA. The viral genome contains a single long open reading frame of 9,315 nucleotides encoding a polyprotein of 3,105 amino acids. The polyprotein was predicted to be cleaved into ten mature proteins by three viral proteases. Complete genome sequence comparison and phylogenetic analysis indicated that NLSYV-ZZ was most closely related to narcissus yellow stripe virus (NYSV), which was also isolated from narcissus. These viruses shared 69.9 % identity in their complete nucleotide sequences and 77.0 % identity in their polyprotein amino acid sequences.
authors.affiliation: Inspection and Quarantine Technology Center of Fujian Entry-Exit Inspection and Quarantine Bureau, Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Fuzhou 350001, People's Republic of China.
authors.forename: Shuang-Qing
authors.initials: SQ
authors.lastname: Lin
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Shen
authors.forename: Fang-Luan
authors.initials: FL
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Cai
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xie
authors.forename: Zu-Jian
authors.initials: ZJ
authors.lastname: Wu
mesh_terms: D002681:China; D016000:Cluster Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D031427:Narcissus / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D020815:Polyproteins / Q000235:genetics; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins