title: Reclassification and subtyping of so-called malignant fibrous histiocytoma of bone: comparison with cytogenetic features.
abstract: BACKGROUND
  The diagnostic entity malignant fibrous histiocytoma (MFH) of bone is, like its soft tissue counterpart, likely to be a misnomer, encompassing a variety of poorly differentiated sarcomas. When reviewing a series of 57 so-called MFH of bone within the framework of the EuroBoNeT consortium according to up-to-date criteria and ancillary immunohistochemistry, a fourth of all tumors were reclassified and subtyped.
  
  
  METHODS
  In the present study, the cytogenetic data on 11 of these tumors (three myoepithelioma-like sarcomas, two leiomyosarcomas, one undifferentiated pleomorphic sarcoma with incomplete myogenic differentiation, two undifferentiated pleomorphic sarcomas, one osteosarcoma, one spindle cell sarcoma, and one unclassifiable biphasic sarcoma) are presented.
  
  
  RESULTS
  All tumors were high-grade lesions and showed very complex karyotypes. Neither the overall pattern (ploidy level, degree of complexity) nor specific cytogenetic features distinguished any of the subtypes. The subgroup of myoepithelioma-like sarcomas was further investigated with regard to the status of the EWSR1 and FUS loci; however, no rearrangement was found. Nor was any particular aberration that could differentiate any of the subtypes from osteosarcomas detected.
  
  
  CONCLUSIONS
  chromosome banding analysis is unlikely to reveal potential genotype-phenotype correlations between morphologic subtypes among so-called MFH of bone.
authors.affiliation: Department of Clinical Genetics, University and Regional Laboratories, Skåne University Hospital, Lund University, Lund, Sweden. fredrik.mertens@med.lu.se.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Mertens
authors.forename: Salvatore
authors.initials: S
authors.lastname: Romeo
authors.forename: Judith Vmg
authors.initials: JV
authors.lastname: Bovée
authors.forename: Roberto
authors.initials: R
authors.lastname: Tirabosco
authors.forename: Nick
authors.initials: N
authors.lastname: Athanasou
authors.forename: Marco
authors.initials: M
authors.lastname: Alberghini
authors.forename: Pancras Cw
authors.initials: PC
authors.lastname: Hogendoorn
authors.forename: Angelo P
authors.initials: AP
authors.lastname: Dei Tos
authors.forename: Raf
authors.initials: R
authors.lastname: Sciot
authors.forename: Henryk A
authors.initials: HA
authors.lastname: Domanski
authors.forename: Kristina
authors.initials: K
authors.lastname: Aström
authors.forename: Nils
authors.initials: N
authors.lastname: Mandahl
authors.forename: Maria
authors.initials: M
authors.lastname: Debiec-Rychter