title: Replication of minute virus of mice in murine cells is facilitated by virally induced depletion of p21.
abstract: The DNA damage response to infection with minute virus of mice (MVM) leads to activated p53; however, p21 levels are reduced via a proteasome-mediated mechanism. This loss was sustained, as virus replicated in infected cells held at the G(2)/M border. Addition of the cyclin-dependent kinase (CDK) inhibitor roscovitine after S-phase entry reduced MVM replication, suggesting that CDK activity was critical for continued viral replication and virus-induced reduction of p21 may thus be necessary to prevent inhibition of CDK.
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri-Columbia, School of Medicine, Columbia, Missouri, USA.
authors.forename: Richard O
authors.initials: RO
authors.lastname: Adeyemi
authors.forename: David J
authors.initials: DJ
authors.lastname: Pintel
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008915:Minute Virus of Mice / Q000502:physiology*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication / Q000502:physiology*
chemical_list: C493957:Cdkn1a protein, mouse; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016159:Tumor Suppressor Protein p53