title: Best temperature for static liver graft storage is 1°C.
abstract: BACKGROUND
  The best storage temperature in liver transplantation remains an unsolved question.
  
  
  METHODS
  After storage for 24h in University of Wisconsin solution at +4°C, +1°C, or -0.5°C, rat livers were subjected, or not, to 15min of warm ischemia, rinsed with Ringer lactate, and subsequently reperfused with oxygenated Krebs-Henseleit buffer.
  
  
  RESULTS
  In the presence of warm ischemia, for livers stored at +4°C, creatine kinase (CK) peaked at 21±5IUg(-1)h(-1), hepatic resistance at 34,700±1500dynscm(-5), bile flow reached 18±4μLg(-1)h(-1) after 10min, and oxygen consumption stabilized at about 25μmolg(-1)h(-1) after 20min. When livers were stored at +1°C, CK and hepatic resistance were lowered, bile production was 33±6μLg(-1)h(-1) (P<0.05 versus +4°C), and oxygen consumption was 105±10μmolg(-1)h(-1) (P<0.001). For livers stored at-0.5°C, results were not statistically different from those of the +1°C group except for bile flow, which was significantly lower. Without warm ischemia, the peak of CK (P=0.015) and the peak hepatic resistance (P<0.001) of the +4°C group were significantly increased compared with the +1°C or -0.5°C groups. However, no difference in bile flow or oxygen consumption was observed. The number of trypan blue-positive nonparenchymal cells (P=0.003) and the gain in liver weight during the reperfusion (P=0.015) were minimal after storage at +1°C.
  
  
  CONCLUSIONS
  Static storage at +1°C improved liver function compared with +4°C or -0.5°C. Main beneficial effect was observed with parameters reflecting sinusoidal cells injury.
authors.affiliation: Laboratoire de Pharmacie Galénique EA 4466, Faculté des Sciences Pharmaceutiques et Biologiques de l'Université Paris Descartes, 4, avenue de l'Observatoire, 75270 Paris Cedex 06, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Charrueau
authors.forename: Nathalie
authors.initials: N
authors.lastname: Neveux
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Chaumeil
authors.forename: Laurent
authors.initials: L
authors.lastname: Hannoun
authors.forename: Luc
authors.initials: L
authors.lastname: Cynober
authors.forename: Eric
authors.initials: E
authors.lastname: Savier
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001646:Bile / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000097:blood; D016031:Liver Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D010101:Oxygen Consumption; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature; D014655:Vascular Resistance
chemical_list: D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase