title: α-1 Adrenergic receptors are localized on presynaptic elements in the nucleus accumbens and regulate mesolimbic dopamine transmission.
abstract: Brainstem noradrenergic neurons innervate the mesocorticolimbic reward pathway both directly and indirectly, with norepinephrine facilitating dopamine (DA) neurotransmission via α1-adrenergic receptors (α1ARs). Although α1AR signaling in the prefrontal cortex (PFC) promotes mesolimbic transmission and drug-induced behaviors, the potential contribution of α1ARs in other parts of the pathway, such as the ventral tegmental area (VTA) and nucleus accumbens (NAc), has not been investigated before. We found that local blockade of α1ARs in the medial NAc shell, but not the VTA, attenuates cocaine- and morphine-induced locomotion. To determine the neuronal substrates that could mediate these effects, we analyzed the cellular, subcellular, and subsynaptic localization of α1ARs and characterized the chemical phenotypes of α1AR-containing elements within the mesocorticolimbic system using single and double immunocytochemical methods at the electron microscopic (EM) level. We found that α1ARs are found mainly extra-synaptically in axons and axon terminals in the NAc and are enriched in glutamatergic and dopaminergic elements. α1ARs are also abundant in glutamatergic terminals in the PFC, and in GABA-positive terminals in the VTA. In line with these observations, microdialysis experiments revealed that local blockade of α1ARs attenuated the increase in extracellular DA in the medial NAc shell following administration of cocaine. These data indicate that local α1ARs control DA transmission in the medial NAc shell and behavioral responses to drugs of abuse.
authors.affiliation: Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Darlene A
authors.initials: DA
authors.lastname: Mitrano
authors.forename: Jason P
authors.initials: JP
authors.lastname: Schroeder
authors.forename: Yoland
authors.initials: Y
authors.lastname: Smith
authors.forename: James J
authors.initials: JJ
authors.lastname: Cortright
authors.forename: Nancy
authors.initials: N
authors.lastname: Bubula
authors.forename: Paul
authors.initials: P
authors.lastname: Vezina
authors.forename: David
authors.initials: D
authors.lastname: Weinshenker
mesh_terms: D000818:Animals; D003042:Cocaine / Q000008:administration & dosage; D004298:Dopamine / Q000502:physiology*; D057967:Infusions, Intraventricular; D008032:Limbic System / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009020:Morphine / Q000008:administration & dosage; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D009714:Nucleus Accumbens / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D017729:Presynaptic Terminals / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018340:Receptors, Adrenergic, alpha-1 / Q000032:analysis / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*
chemical_list: D018340:Receptors, Adrenergic, alpha-1; D009020:Morphine; D003042:Cocaine; D004298:Dopamine