title: Effect of Calea serrata Less. n-hexane extract on acetylcholinesterase of larvae ticks and brain Wistar rats.
abstract: Acetylcholinesterase (AChE), an enzyme that hydrolyses acetylcholine (ACh) at cholinergic synapses, is a target for pesticides and its inhibition by organophosphates leads to paralysis and death of arthropods. It has been demonstrated that the n-hexane extract of Calea serrata had acaricidal activity against larvae of Rhipicephalus (Boophilus) microplus and Rhipicephalus sanguineus. The aim of the present study was to understand the mechanism of the acaricidal action of C. serrata n-hexane extract are specifically to investigate the in vitro anticholinesterase activity on larvae of R. microplus and in brain structures of male Wistar rats. The n-hexane extract significantly inhibited in vitro acetylcholinesterase activity in R. microplus larvae and rat brain structures. The results confirm that inhibition of acetylcholinesterase is a possible mechanism of action of hexane extract at C. serrata.
authors.affiliation: Programa de Pós-graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, 90035-003 Porto Alegre, RS, Brazil.
authors.forename: Vera Lucia Sardá
authors.initials: VL
authors.lastname: Ribeiro
authors.forename: Cláudia
authors.initials: C
authors.lastname: Vanzella
authors.forename: Felipe dos Santos
authors.initials: Fdos S
authors.lastname: Moysés
authors.forename: Jaqueline Campiol Dos
authors.initials: JC
authors.lastname: Santos
authors.forename: João Ricardo Souza
authors.initials: JR
authors.lastname: Martins
authors.forename: Gilsane Lino
authors.initials: GL
authors.lastname: von Poser
authors.forename: Ionara Rodrigues
authors.initials: IR
authors.lastname: Siqueira
mesh_terms: D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006586:Hexanes / Q000737:chemistry*; D007814:Larva / Q000187:drug effects / Q000201:enzymology; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D048508:Rhipicephalus sanguineus / Q000187:drug effects* / Q000201:enzymology
chemical_list: D002800:Cholinesterase Inhibitors; D006586:Hexanes; D010936:Plant Extracts; C026385:n-hexane