title: Identification of serum biomarker signatures associated with pancreatic cancer.
abstract: Pancreatic cancer is an aggressive disease with poor prognosis, due, in part, to the lack of disease-specific biomarkers that could afford early and accurate diagnosis. With a recombinant antibody microarray platform, targeting mainly immunoregulatory proteins, we screened sera from 148 patients with pancreatic cancer, chronic pancreatitis, autoimmune pancreatitis (AIP), and healthy controls (N). Serum biomarker signatures were derived from training cohorts and the predictive power was evaluated using independent test cohorts. The results identified serum portraits distinguishing pancreatic cancer from N [receiver operating characteristics area under the curve (AUC) of 0.95], chronic pancreatitis (0.86), and AIP (0.99). Importantly, a 25-serum biomarker signature discriminating pancreatic cancer from the combined group of N, chronic pancreatitis, and AIP was determined. This signature exhibited a high diagnostic potential (AUC of 0.88). In summary, we present the first prevalidated, multiplexed serum biomarker signature for diagnosis of pancreatic cancer that may improve diagnosis and prevention in premalignant diseases and in screening of high-risk individuals.
authors.affiliation: Department of Immunotechnology, Lund University, Lund, Sweden.
authors.forename: Christer
authors.initials: C
authors.lastname: Wingren
authors.forename: Anna
authors.initials: A
authors.lastname: Sandström
authors.forename: Ralf
authors.initials: R
authors.lastname: Segersvärd
authors.forename: Anders
authors.initials: A
authors.lastname: Carlsson
authors.forename: Roland
authors.initials: R
authors.lastname: Andersson
authors.forename: Matthias
authors.initials: M
authors.lastname: Löhr
authors.forename: Carl A K
authors.initials: CA
authors.lastname: Borrebaeck
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D021441:Carcinoma, Pancreatic Ductal / Q000097:blood*; D006801:Humans; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000097:blood*; D010195:Pancreatitis / Q000175:diagnosis; D050500:Pancreatitis, Chronic / Q000097:blood
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor