title: Evaluation of collagen alteration after topical photodynamic therapy (PDT) using second harmonic generation (SHG) microscopy--in vivo study in a mouse model.
abstract: Topical photodynamic therapy (PDT) mediated by 5-aminolevulinic acid (ALA) has been used for the treatment of age-related skin lesions for therapeutic or cosmetic purposes. The modulation of collagen component and structure might play a significant role in influencing treatment outcomes of PDT. In this study, the effect of multi-session low dose ALA PDT on skin rejuvenation was examined in a hairless mouse model. Changes in collagen and skin texture were investigated by histological examination and in vivo second harmonic generation (SHG) microscopy. Results indicated that multi-session PDT could improve the collagen density and arrangement of skin tissue. SHG microscopy combined with quantitative collagen analysis could provide a useful tool for the evaluation of collagen alteration induced by ALA PDT.
authors.affiliation: Shanghai Skin Diseases Hospital, Shanghai 200050, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Lv
authors.forename: Zu-Fang
authors.initials: ZF
authors.lastname: Huang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Wang
authors.forename: Ju-Qiang
authors.initials: JQ
authors.lastname: Lin
authors.forename: Guan-Nan
authors.initials: GN
authors.lastname: Chen
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: Chen
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Wang
mesh_terms: D000287:Administration, Topical; D021542:Amino Acids, Neutral / Q000494:pharmacology*; D000818:Animals; D003094:Collagen / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*; D005260:Female; D007089:Image Enhancement / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D008853:Microscopy / Q000379:methods*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects
chemical_list: D021542:Amino Acids, Neutral; D017319:Photosensitizing Agents; C013809:5-aminovaleric acid; D003094:Collagen