title: An integrated electrochemical device based on immunochromatographic test strip and enzyme labels for sensitive detection of disease-related biomarkers.
abstract: A novel electrochemical biosensing device that integrates an immunochromatographic test strip and a screen-printed electrode (SPE) connected to a portable electrochemical analyzer was presented for rapid, sensitive, and quantitative detection of disease-related biomarker in human blood samples. The principle of the sensor is based on sandwich immunoreactions between a biomarker and a pair of its antibodies on the test strip, followed by highly sensitive square-wave voltammetry (SWV) detection. Horseradish peroxidase (HRP) was used as a signal reporter for electrochemical readout. Hepatitis B surface antigen (HBsAg) was employed as a model protein biomarker to demonstrate the analytical performance of the sensor in this study. Some critical parameters governing the performance of the sensor were investigated in detail. Under optimal conditions, this sensor was capable of detecting a minimum of 0.3 ng mL(-1) (S/N=3) HBsAg with a wide linear concentration range from 1 to 500 ng mL(-1). The sensor was further utilized to detect HBsAg spiked in human plasma with an average recovery of 91.3%. In comparison, a colorimetric immunochromatographic test strip assay (ITSA) was also conducted. The result shows that the SWV detection in the electrochemical sensor is much more sensitive for the quantitative determination of HBsAg than the colorimetric detection, indicating that such a sensor is a promising platform for rapid and sensitive point-of-care testing/screening of disease-related biomarkers in a large population.
authors.affiliation: Department of Chemistry and Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China.
authors.forename: Zhe-Xiang
authors.initials: ZX
authors.lastname: Zou
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Yao-Qun
authors.initials: YQ
authors.lastname: Li
authors.forename: Yuehe
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D015415:Biomarkers / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods*; D055664:Electrochemical Techniques; D004566:Electrodes; D004867:Equipment Design; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D045663:Quantum Dots; D011934:Reagent Strips / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D000906:Antibodies; D015415:Biomarkers; D006514:Hepatitis B Surface Antigens; D011934:Reagent Strips; D006735:Horseradish Peroxidase