title: Short-term glucose variability in healthy volunteers is not associated with raised oxidative stress markers.
abstract: It is unknown whether glycaemic variability adds to the risk of microvascular complications of diabetes over and above the mean glucose value for a patient. We examined the effect of purposefully induced short-term glycaemic variability on oxidative stress markers. Eleven healthy subjects underwent three sequential glycaemic states; sustained hyperglycaemia, sustained euglycaemia and variable glycaemia, using glycaemic clamps for 3 h. Twenty-four hours urinary 8-isoprostane-PGF2α was measured before and after each glycaemic state to assess oxidative stress. The median and interquartile range of the urinary 8-iso-PGF2α in ng/24 h were (1373, 513), (996, 298) and (1227, 472) for the euglycaemic, hyperglycaemic and variable states, respectively. There was no significant difference in urinary isoprostanes between the three different states; mean ranks 20.9, 11.9 and 18.2 for the euglycaemic state, hyperglycaemic state and glycaemic variability state, respectively, p = 0.083. In conclusion, we did not see a significant increase in the urinary isoprostanes when glycaemic variability was induced under controlled conditions in healthy individuals.
authors.forename: A
authors.initials: A
authors.lastname: Wakil
authors.forename: K A
authors.initials: KA
authors.lastname: Smith
authors.forename: S L
authors.initials: SL
authors.lastname: Atkin
authors.forename: E S
authors.initials: ES
authors.lastname: Kilpatrick
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001786:Blood Glucose / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D006943:Hyperglycemia / Q000652:urine*; D008297:Male; D018384:Oxidative Stress; D013997:Time Factors
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost