title: Protective effect of Heliotropium eichwaldi against cisplatin-induced nephrotoxicity in mice.
abstract: OBJECTIVE
  The aim of the present study was to evaluate the nephroprotective effect of methanolic extract of Heliotropium eichwaldii (MHE) in mice with cisplatin-induced acute renal damage.
  
  
  METHODS
  Nephrotoxicity was induced by a single intraperitoneal injection of cisplatin (16mg/kg). Swiss albino mice were injected with vehicle, cisplatin, cisplatin plus MHE 200 mg/kg and cisplatin plus MHE 400mg/kg, respectively. MHE was administered for 7 d at a dose of 200 and 400 mg/kg per day orally starting 4 d before cisplatin injection. Animals were sacrificed 3d after treatment and blood as well as kidney tissue was isolated and analyzed. The various parameters such as blood urea nitrogen (BUN), serum creatinine (CRE), malondialdehyde (MDA), and catalase (CAT) and superoxide dismutase (SOD) activities were analyzed.
  
  
  RESULTS
  MHE treatment significantly reduced BUN and serum CRE levels elevated by cisplatin administration (P<0.05). Also, it significantly attenuated cisplatin-induced increase in MDA level and improved the decreased CAT and SOD activities in renal cortical homogenates (P<0.05). Additionally, histopathological examination and scoring showed that MHE markedly ameliorated cisplatin-induced renal tubular necrosis.
  
  
  CONCLUSIONS
  MHE can be considered a potential candidate for protection of nephrotoxicity induced by cisplatin.
authors.affiliation: Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar , Haryana, India. prof.sharmask@gmail.com
authors.forename: Surendra Kr
authors.initials: SK
authors.lastname: Sharma
authors.forename: Naveen
authors.initials: N
authors.lastname: Goyal
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D001806:Blood Urea Nitrogen; D002945:Cisplatin / Q000009:adverse effects; D028162:Heliotropium / Q000737:chemistry*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007684:Kidney Tubules / Q000473:pathology; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D010936:Plant Extracts; D002945:Cisplatin