title: Bronchorelaxant, mast cell stabilizing, anti-inflammatory and antioxidant activity of Randia dumetorum (Retz.) Lamk. extracts.
abstract: Randia dumetorum (RD) fruits in different form have been ethnopharmacologically reported to possess antiasthmatic property. Therefore, present study was undertaken to evaluate two different extracts of RD i.e., ethyl acetate (RD-EA) and methanol (RD-ME) for bronchorelaxant, anti-inflammatory, mast cell stabilizing and antioxidant effect along with safety margin, according to OECD guidelines for toxicity. RD-ME and RD-EA (1 mg/mL) exhibited 68.75 and 57.39% inhibition of contraction against acetylcholine, while against histamine induced contraction, inhibition observed was 100 and 78.13%, respectively. Moreover, extracts attenuated the experimentally induced inflammation at 200 mg/kg with % inhibition of 41.62 by RD-ME and 30.36 by RD-EA in carrageenan model, while in egg albumin model RD-ME and RD-EA exhibited % inhibition of 48.31 and 33.75, respectively. In addition, RD-ME and RD-EA at 100 microg/mL demonstrated significant decrease in histamine release of 08.31 and 16.71 in C-48/80 induced mast cell degradation. RD extracts also exhibited antioxidant activity in DPPH, reducing power and metal chelation method, along with safe margin for oral administration as observed in acute toxicity evaluation.
authors.affiliation: Sharad Pawar College of Pharmacy, Wanadongari, Hingna Road, Nagpur-441 110 (M. S.), India. am_mahavir@rediffmail.com
authors.forename: Mahavir H
authors.initials: MH
authors.lastname: Ghante
authors.forename: Kishore P
authors.initials: KP
authors.lastname: Bhusari
authors.forename: Nandkishore J
authors.initials: NJ
authors.lastname: Duragkar
authors.forename: Nishant S
authors.initials: NS
authors.lastname: Jain
authors.forename: Amol S
authors.initials: AS
authors.lastname: Warokar
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001993:Bronchodilator Agents / Q000737:chemistry / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D006168:Guinea Pigs; D008407:Mast Cells / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D019666:Rubiaceae / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001993:Bronchodilator Agents; D010936:Plant Extracts