title: Development and characterization of a novel CD19CherryLuciferase (CD19CL) transgenic mouse for the preclinical study of B-cell lymphomas.
abstract: OBJECTIVE
  To generate a transgenic mouse that when crossed with spontaneous mouse models of lymphoma will allow for quantitative in vivo measurement of tumor burden over the entire spectrum of the disease and or response to therapy in a "disease" or lymphoma subtype-specific manner.
  
  
  METHODS
  We developed a novel genetically engineered transgenic mouse using a CherryLuciferase fusion gene targeted to the CD19 locus to achieve B-cell-restricted fluorescent bioluminescent emission in transgenic mouse models of living mice. The use of a dual function protein enables one to link the in vivo analysis via bioluminescence imaging to cell discriminating ex vivo analyses via fluorescence emission.
  
  
  RESULTS
  The spatiotemporal tracking of B-cell lymphoma growth and the response of an established B-cell lymphoma to a drug known to induce remission was evaluated in a double transgenic animal obtained by crossing the CD19CherryLuciferase transgenic mouse to a mouse model of an aggressive B-cell lymphoma. The observations validated the use of the CD19CherryLuciferase transgenic mouse in the assessment of an active drug routinely used in the treatment of lymphoproliferative malignancies.
  
  
  CONCLUSIONS
  The transgenic mouse described here is the first of its kind, intended to be used to hasten translational studies of novel agents in lymphoma, with the intent that understanding the relevant pharmacology before clinical study will accelerate successful development in clinical studies.
authors.affiliation: Center for Lymphoid Malignancies and Department of Medicine, Columbia University, NY, USA.
authors.forename: Luigi
authors.initials: L
authors.lastname: Scotto
authors.forename: Marianna
authors.initials: M
authors.lastname: Kruithof-de Julio
authors.forename: Luca
authors.initials: L
authors.lastname: Paoluzzi
authors.forename: Matko
authors.initials: M
authors.lastname: Kalac
authors.forename: Enrica
authors.initials: E
authors.lastname: Marchi
authors.forename: Jairo Baquero
authors.initials: JB
authors.lastname: Buitrago
authors.forename: Jennifer
authors.initials: J
authors.lastname: Amengual
authors.forename: Michael M
authors.initials: MM
authors.lastname: Shen
authors.forename: Owen A
authors.initials: OA
authors.lastname: O'Connor
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000235:genetics*; D001402:B-Lymphocytes / Q000378:metabolism*; D005453:Fluorescence; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D016393:Lymphoma, B-Cell / Q000188:drug therapy / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D018941:Antigens, CD19; D011994:Recombinant Proteins; D008156:Luciferases