title: A simple assay of taurine concentrations in food and biological samples using taurine dioxygenase.
abstract: Taurine demonstrates various physiological functions and pharmacological actions. A successful application of taurine dioxygenase (EC 1.14.11.17) for taurine determination is described. The gene encoding taurine dioxygenase was cloned from Escherichia coli strain K-12, and the enzyme was used to determine taurine in commercially available beverages and some biological samples. The measured values obtained using the current method are close to the declared values with the precolumn derivatization ultra-performance liquid chromatography (UPLC) procedure. Taurine dioxygenase can be used for taurine determination in food control, biological research, and diagnoses based on urinary taurine concentration.
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.
authors.forename: Motoki
authors.initials: M
authors.lastname: Matsuda
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Asano
mesh_terms: D000818:Animals; D001628:Beverages / Q000032:analysis*; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D004926:Escherichia coli; D005504:Food Analysis; D006801:Humans; D006899:Mixed Function Oxygenases / Q000737:chemistry*; D033561:Penaeidae / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D012758:Shellfish / Q000032:analysis*; D013654:Taurine / Q000032:analysis* / Q000652:urine
chemical_list: D011994:Recombinant Proteins; D013654:Taurine; D004228:Dithionitrobenzoic Acid; D006899:Mixed Function Oxygenases; C433064:taurine-alpha-ketoglutarate dioxygenase