title: Increased mast cell density and airway responses to allergic and non-allergic stimuli in a sheep model of chronic asthma.
abstract: BACKGROUND
  Increased mast cell (MC) density and changes in their distribution in airway tissues is thought to contribute significantly to the pathophysiology of asthma. However, the time sequence for these changes and how they impact small airway function in asthma is not fully understood. The aim of the current study was to characterise temporal changes in airway MC density and correlate these changes with functional airway responses in sheep chronically challenged with house dust mite (HDM) allergen.
  
  
  RESULTS
  MC density was examined on lung tissue from four spatially separate lung segments of allergic sheep which received weekly challenges with HDM allergen for 0, 8, 16 or 24 weeks. Lung tissue was collected from each segment 7 days following the final challenge. The density of tryptase-positive and chymase-positive MCs (MC(T) and MC(TC) respectively) was assessed by morphometric analysis of airway sections immunohistochemically stained with antibodies against MC tryptase and chymase. MC(T) and MC(TC) density was increased in small bronchi following 24 weeks of HDM challenges compared with controls (P<0.05). The MC(TC)/MC(T) ratio was significantly increased in HDM challenged sheep compared to controls (P<0.05). MC(T) and MC(TC) density was inversely correlated with allergen-induced increases in peripheral airway resistance after 24 weeks of allergen exposure (P<0.05). MC(T) density was also negatively correlated with airway responsiveness after 24 challenges (P<0.01).
  
  
  CONCLUSIONS
  MC(T) and MC(TC) density in the small airways correlates with better lung function in this sheep model of chronic asthma. Whether this finding indicates that under some conditions mast cells have protective activities in asthma, or that other explanations are to be considered requires further investigation.
authors.affiliation: Centre for Animal Biotechnology, Veterinary Science, University of Melbourne, Parkville, Australia.
authors.forename: Joanne
authors.initials: J
authors.lastname: Van der Velden
authors.forename: Donna
authors.initials: D
authors.lastname: Barker
authors.forename: Garry
authors.initials: G
authors.lastname: Barcham
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Koumoundouros
authors.forename: Kenneth
authors.initials: K
authors.lastname: Snibson
mesh_terms: D000485:Allergens; D000818:Animals; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000276:immunology* / Q000473:pathology* / Q000503:physiopathology; D016084:Bronchoconstriction / Q000276:immunology; D002452:Cell Count; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology / Q000473:pathology; D005260:Female; D008168:Lung / Q000276:immunology / Q000473:pathology; D008407:Mast Cells / Q000276:immunology* / Q000473:pathology*; D039981:Pyroglyphidae / Q000276:immunology; D012756:Sheep
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides