title: A selective PMCA inhibitor does not prolong the electroolfactogram in mouse.
abstract: BACKGROUND
  Within the cilia of vertebrate olfactory receptor neurons, Ca(2+) accumulates during odor transduction. Termination of the odor response requires removal of this Ca(2+), and prior evidence suggests that both Na(+)/Ca(2+) exchange and plasma membrane Ca(2+)-ATPase (PMCA) contribute to this removal.
  
  
  RESULTS
  In intact mouse olfactory epithelium, we measured the time course of termination of the odor-induced field potential. Replacement of mucosal Na(+) with Li(+), which reduces the ability of Na(+)/Ca(2+) exchange to expel Ca(2+), prolonged the termination as expected. However, treating the epithelium with the specific PMCA inhibitor caloxin 1b1 caused no significant increase in the time course of response termination.
  
  
  CONCLUSIONS
  Under these experimental conditions, PMCA does not contribute detectably to the termination of the odor response.
authors.affiliation: Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, United States of America.
authors.forename: Edwin R
authors.initials: ER
authors.lastname: Griff
authors.forename: Nancy K
authors.initials: NK
authors.lastname: Kleene
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Kleene
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002923:Cilia / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008094:Lithium / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D009812:Odorants; D009831:Olfactory Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D010455:Peptides / Q000494:pharmacology; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D012964:Sodium / Q000378:metabolism; D019831:Sodium-Calcium Exchanger / Q000378:metabolism
chemical_list: D010455:Peptides; D019831:Sodium-Calcium Exchanger; C510327:caloxin 1b1; D008094:Lithium; D012964:Sodium; D053499:Plasma Membrane Calcium-Transporting ATPases; D002118:Calcium