title: Formation of nano-bio-complex as nanomaterials dispersed in a biological solution for understanding nanobiological interactions.
abstract: Information on how cells interface with nanomaterials in biological environments has important implications for the practice of nanomedicine and safety consideration of nanomaterials. However, our current understanding of nanobiological interactions is still very limited. Here, we report the direct observation of nanomaterial bio-complex formation (other than protein corona) from nanomaterials dispersed in biologically relevant solutions. We observed highly selective binding of the components of cell culture medium and phosphate buffered saline to ZnO and CuO nanoparticles, independent of protein molecules. Our discoveries may provide new insights into the understanding of how cells interact with nanomaterials.
authors.affiliation: State Key Laboratory of Silicon Materials, MOE Key Laboratory of Macromolecule Synthesis and Functionalization & Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China. msxu@zju.edu.cn
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Hideo
authors.initials: H
authors.lastname: Iwai
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Mei
authors.forename: Daisuke
authors.initials: D
authors.lastname: Fujita
authors.forename: Huanxing
authors.initials: H
authors.lastname: Su
authors.forename: Hongzheng
authors.initials: H
authors.lastname: Chen
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Hanagata
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D044967:Serum / Q000737:chemistry / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry / Q000378:metabolism; D012996:Solutions / Q000737:chemistry* / Q000378:metabolism; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry* / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D012996:Solutions; D012965:Sodium Chloride; D003300:Copper; D015034:Zinc Oxide; C030973:cupric oxide