title: Adsorption of chain type-specific ABO antibodies on Sepharose-linked A and B tetrasaccharides.
abstract: BACKGROUND
  Antigen-specific removal of anti-A and anti-B on immunoadsorption columns carrying the blood group A and B trisaccharides is one important component of some protocols used in ABO-incompatible organ transplantation. Because ABO antibodies exist requiring parts of the core saccharide chain for binding, the anti-A and -B-binding capacity of individual and combined, Sepharose-linked Types 1 through 4 A and B tetrasaccharides with that of the A and B trisaccharides was compared.
  
  
  METHODS
  Sepharose-linked A and B tri- and tetrasaccharides were used to adsorb anti-A and -B from pooled blood group O serum. Remaining chain type-specific anti-A and -B were detected and quantified in enzyme-linked immunosorbent assays using wells coated with neoglycoproteins or recombinant mucins carrying A and B determinants on defined core saccharide chains.
  
  
  RESULTS
  Significantly more anti-A Type 3- and 4-specific immunoglobulin (Ig)G remained after adsorption on the A trisaccharide and the A Type 1 and A Type 2 tetrasaccharide than after adsorption on the A Types 3 and 4 tetrasaccharides. Selective adsorption of chain type-specific IgG anti-B was detected on Sepharose-linked B tetrasaccharides. In contrast, there were no chain type-specific IgM anti-A or -B. A combination of the A or B tetrasaccharides adsorbed a larger fraction of the IgG anti-A and -B repertoires than the corresponding trisaccharides.
  
  
  CONCLUSIONS
  There are chain type-specific anti-A and anti-B IgG, and an adsorber based on a combination of Types 1 through 4 A or B tetrasaccharides will be a more efficient adsorber than an adsorber based on the A or B trisaccharides.
authors.affiliation: AbSorber AB, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden.
authors.forename: Linda
authors.initials: L
authors.lastname: Lindberg
authors.forename: Katharina
authors.initials: K
authors.lastname: Theinert
authors.forename: Jining
authors.initials: J
authors.lastname: Liu
authors.forename: Jan
authors.initials: J
authors.lastname: Holgersson
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D001788:Blood Grouping and Crossmatching; D004797:Enzyme-Linked Immunosorbent Assay; D006648:Histocompatibility / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D007163:Immunosorbent Techniques; D016030:Kidney Transplantation / Q000276:immunology; D009844:Oligosaccharides; D012685:Sepharose; D014181:Transplantation Immunology
chemical_list: D000017:ABO Blood-Group System; D000906:Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009844:Oligosaccharides; D012685:Sepharose