title: Reflectance confocal microscopy for quantification of Sarcoptes scabiei in Norwegian scabies.
abstract: OBJECTIVE
  In vivo reflectance-mode confocal microscopy (RCM) can be used for the diagnosis of scabies. This study quantifies S. scabiei and its eggs and droppings in a patient affected by Norwegian Scabies (NS), and describes their distribution within the epidermis and in different body areas.
  
  
  METHODS
  Different skin sites were randomly chosen in four sections (head, upper limbs, trunk and inferior limbs) of the body surface area (BSA) to acquire a total of 60 RCM z-stacks. The number of mites and eggs, the presence of droppings, as well as the minimum epidermal depth at which mites, eggs and faeces were detectable, was established for each z-stack. The total number of mites and eggs on the entire BSA was calculated considering the weighted mean for the four sections of the BSA.
  
  
  RESULTS
  A total of 15.8 millions of S. scabiei and 7.2 millions of eggs were calculated. Mites, eggs and faeces were homogeneously distributed all over the body surface. Droppings, easily recognized by the RCM, were present in more than an half of the analyzed cutaneous sites and were associated with the presence of parasites (chi-squared test, P = 0.002).
  
  
  CONCLUSIONS
  Our study illustrates the ability of RCM to identify, locate, and quantify the various forms of S. scabiei in human skin. NS is an extremely contagious disease, considering that the number of mites can be around 15.8 millions. Moreover, all areas of the body are parasitized in NS, including the face.
authors.affiliation: Service de Dermatologie Hôpital Nord, Cedex 2, Saint Etienne, France.
authors.forename: E
authors.initials: E
authors.lastname: Cinotti
authors.forename: J L
authors.initials: JL
authors.lastname: Perrot
authors.forename: B
authors.initials: B
authors.lastname: Labeille
authors.forename: P
authors.initials: P
authors.lastname: Vercherin
authors.forename: C
authors.initials: C
authors.lastname: Chol
authors.forename: E
authors.initials: E
authors.lastname: Besson
authors.forename: F
authors.initials: F
authors.lastname: Cambazard
mesh_terms: D000818:Animals; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods*; D010270:Parasite Egg Count; D012520:Sarcoptes scabiei; D012532:Scabies / Q000469:parasitology*