title: Relaxant effects of Aike Mixture on isolated bladder and prostatic urethral smooth muscle of rabbits.
abstract: OBJECTIVE
  To observe the relaxant effect of Aike Mixture (AKM) on isolated bladder and prostatic urethral smooth muscle of rabbits.
  
  
  METHODS
  The isolated bladder and prostatic urethral smooth muscle from male rabbits were placed in a Magnus bath and smooth muscle contraction was measured using a biological signal acquisition and analysis system. The effects of AKM in combination with methoxyamine, carbachol and CaCl2 on the contractile tension of muscle strips were determined by cumulative dosing.
  
  
  RESULTS
  AKM dose-dependently reduced contractile tension of bladder trigone smooth muscle (r=0.831, P<0.05), reduced contractile wave amplitude (r=0.837, P<0.05) and decreased contractile frequency (r=-0.917, P<0.01). AKM significantly inhibited the increases in smooth muscle contraction induced by methoxyamine, carbachol and CaCl2.
  
  
  CONCLUSIONS
  AKM dose-dependently inhibited the contraction of rabbit isolated bladder and prostatic urethral smooth muscle by antagonizing α1-adrenergic receptors and M-cholinergic receptors.
authors.affiliation: People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, 350004, China, zmj83@tom.com.
authors.forename: Min-jian
authors.initials: MJ
authors.lastname: Zhang
authors.forename: Ya-lei
authors.initials: YL
authors.lastname: Shi
authors.forename: Wan-jun
authors.initials: WJ
authors.lastname: Cheng
authors.forename: Zhen-wei
authors.initials: ZW
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Lin
mesh_terms: D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006898:Hydroxylamines / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D009465:Neuromuscular Agents / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000378:metabolism; D014521:Urethra / Q000187:drug effects / Q000502:physiology*; D001743:Urinary Bladder / Q000187:drug effects / Q000502:physiology*
chemical_list: D004365:Drugs, Chinese Herbal; D006898:Hydroxylamines; D009465:Neuromuscular Agents; D018340:Receptors, Adrenergic, alpha-1; D011976:Receptors, Muscarinic; C557891:aike; D002217:Carbachol; C005214:methoxyamine; D002122:Calcium Chloride