title: The interaction between hepatitis B virus X protein and AIB1 oncogene is required for the activation of NFκB signal transduction.
abstract: We identified the interaction between HBV X (HBx) protein and the oncogene AIB1 (amplified in breast cancer 1). A serine/proline motif (SSPSPS) in HBx was found to be required for the interaction. Two LXD motifs [LLXX(X)L, X means any amino acids], LLRNSL and LLDQLHTLL in AIB1 were also found to be involved in the HBx-AIB1 interaction. The HBx-AIB1 interaction was important for the activation of NFκB signal transduction, the HBx mutant that did not interact with AIB1showed dramatically lower NFκB activation activity than the WT HBx. These findings contribute to the new understanding on signal transduction activation mechanisms of HBx.
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB,Canada.
authors.forename: Andy
authors.initials: A
authors.lastname: Hong
authors.forename: Dong Dong
authors.initials: DD
authors.lastname: Han
authors.forename: Christine J
authors.initials: CJ
authors.lastname: Wright
authors.forename: Tanya
authors.initials: T
authors.lastname: Burch
authors.forename: Jessica
authors.initials: J
authors.lastname: Piper
authors.forename: Carla
authors.initials: C
authors.lastname: Osiowy
authors.forename: Chunjin
authors.initials: C
authors.lastname: Gao
authors.forename: Shirley
authors.initials: S
authors.lastname: Chiang
authors.forename: Travis
authors.initials: T
authors.lastname: Magill
authors.forename: Kevin
authors.initials: K
authors.lastname: Dick
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Booth
authors.forename: Xuguang
authors.initials: X
authors.lastname: Li
authors.forename: Runtao
authors.initials: R
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D002273:Carcinogens; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D017124:Conserved Sequence; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016328:NF-kappa B / Q000378:metabolism*; D056921:Nuclear Receptor Coactivator 3 / Q000235:genetics / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D012694:Serine / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D002273:Carcinogens; D016328:NF-kappa B; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D012694:Serine; C538866:NCOA3 protein, human; D056921:Nuclear Receptor Coactivator 3