title: Sparassis crispa suppresses mast cell-mediated allergic inflammation:             Role of calcium, mitogen-activated protein kinase and nuclear factor-κB.
abstract: Allergic inflammatory disease such as food allergy, asthma and atopic dermatitis             are increasing worldwide. In this study, we investigated the effect of water extract             of Sparassis crispa (WESC) Fr. (Aphyllophoromycetideae) on mast cell-mediated             allergic inflammation and the possible mechanisms of action. WESC inhibited compound             48/80-induced systemic anaphylaxis and serum histamine release in mice. WESC decreased             immunoglobulin E (IgE)-mediated passive cutaneous anaphylaxis. Additionally, WESC             reduced histamine release and intracellular calcium in human mast cells activated             by phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187. WESC decreased             PMA and A23187-stimulated expression of pro-inflammatory cytokines, such as tumor             necrosis factor (TNF)-α, inlerleukin (IL)-6 and IL-1β. The inhibitory effect of             WESC on pro-inflammatory cytokines was nuclear factor-κB, extracellular signal-regulated             kinase and p38 mitogen-activated protein kinase-dependent. Our results suggest             potential therapeutic application of WESC in allergic inflammatory diseases.
authors.affiliation: Department of Pharmacology, School of Medicine, Kyungpook National                     University, Daegu 700-422, Republic of Korea.
authors.forename: Hui-Hun
authors.initials: HH
authors.lastname: Kim
authors.forename: Soyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Thoudam S K
authors.initials: TS
authors.lastname: Singh
authors.forename: Jin Kyeong
authors.initials: JK
authors.lastname: Choi
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced / Q000188:drug therapy; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D045424:Complex Mixtures / Q000008:administration & dosage / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006636:Histamine Release / Q000187:drug effects; D006967:Hypersensitivity / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D020072:Polyporales / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D003189:p-Methoxy-N-methylphenethylamine / Q000009:adverse effects
chemical_list: D045424:Complex Mixtures; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D003189:p-Methoxy-N-methylphenethylamine; D020928:Mitogen-Activated Protein Kinases; D002118:Calcium