title: E2F1-dependent methyl cap formation requires RNA pol II phosphorylation.
abstract: Gene expression is a process integral to cell proliferation. The E2F family of transcription factors upregulates expression of transcripts whose products are essential for cell cycle progression. Here, we report that E2F1 promotes gene expression by an additional mechanism, that is, formation of the methyl cap on RNA pol II transcripts. The methyl cap is required for mRNA maturation, expression and stability. We demonstrate that E2F1 increases RNA pol II phosphorylation, which promotes recruitment of the methyl cap synthetic enzymes. Upregulation of RNA pol II phosphorylation is required for E2F1-dependent methyl cap formation.
authors.affiliation: Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee, Dundee, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Aregger
authors.forename: Victoria H
authors.initials: VH
authors.lastname: Cowling
mesh_terms: D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002478:Cells, Cultured; D019175:DNA Methylation; D050687:E2F1 Transcription Factor / Q000378:metabolism*; D015870:Gene Expression; D010766:Phosphorylation; D012319:RNA Polymerase II / Q000378:metabolism*; D051381:Rats
chemical_list: D050687:E2F1 Transcription Factor; D016203:CDC2 Protein Kinase; D012319:RNA Polymerase II