title: Protective role of Brassica olerecea and Eugenia jambolana extracts against H₂O₂ induced cytotoxicity in H9C2 cells.
abstract: This study assesses the efficacy of anthocyanin rich Brassica olerecea leaves (ARCE) and flavonoid rich Eugenia jambolana seed (EJSE) extracts as possible cardioprotective agents against hydrogen peroxide (H(2)O(2)) induced cytotoxicity in H9C2 cells. Presence of ARCE or EJSE resulted in a superior cell viability and cell integrity as revealed by cell viability and lactate dehydrogenase release assays and acridine orange and ethidium bromide staining of control and H(2)O(2) treated H9C2 cells. These extracts were also able to reduce the impact of H(2)O(2) induced lipid peroxidation and depletion of intracellular glutathione. Also, there was an increase in mitochondrial membrane potential and reduced generation of intracellular reactive oxygen species following ARCE or EJSE treatments. These results suggest that ARCE and EJSE are capable of cardioprotective activity due to the high number of anthocyanins and flavonoids in them that are instrumental in lowering intracellular oxidative stress, preventing depletion of cellular antioxidants and improving cell viability.
authors.affiliation: Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The M. S. University of Baroda, Vadodara-390002, Gujarat, India. phyto_met@yahoo.com
authors.forename: Ranjitsinh V
authors.initials: RV
authors.lastname: Devkar
authors.forename: Apurv V
authors.initials: AV
authors.lastname: Pandya
authors.forename: Nancy H
authors.initials: NH
authors.lastname: Shah
mesh_terms: D000818:Animals; D017209:Apoptosis; D001937:Brassica / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D005978:Glutathione / Q000032:analysis; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D032386:Myoblasts, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D027842:Syzygium / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D005978:Glutathione