title: Impact of age and diagnosis on viability during centrifugation and cryopreservation of peripheral blood stem cell products.
abstract: BACKGROUND
  The viability of the hematopoietic stem cells infused to the patient is important for transplant outcome.
  
  
  METHODS
  We evaluated 31 peripheral blood stem cell product collected from 15 patients. We aimed to check the viabilities of the cells from patients with different age and diagnosis, in different stages of the cryopreservation procedure.
  
  
  RESULTS
  We showed a markedly decreased viability rate after centrifugation and addition of DMSO. Percentages of viabilities were similar between young and old patients in each step. Type of hematological malignancy did not make a significant influence on the viability.
  
  
  CONCLUSIONS
  High speed centrifugation has a negative impact on the viability.
authors.affiliation: Department of Hematology, Ankara University School of Medicine, Ankara, Turkey. scivriz@hotmail.com
authors.forename: S
authors.initials: S
authors.lastname: Civriz Bozdag
authors.forename: M
authors.initials: M
authors.lastname: Bay
authors.forename: E
authors.initials: E
authors.lastname: Ayyıldız
authors.forename: P
authors.initials: P
authors.lastname: Topcuoglu
authors.forename: O
authors.initials: O
authors.lastname: Ilhan
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D002470:Cell Survival; D002498:Centrifugation; D015925:Cryopreservation; D005260:Female; D019337:Hematologic Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000628:therapy*; D006412:Hematopoietic Stem Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D014182:Transplantation, Autologous