title: Four SNPs of insulin-induced  gene 1 associated with growth and carcass traits in Qinchuan cattle in China.
abstract: The  insulin-induced gene 1 (Insig-1) is a regulator of lipid metabolism and plays  an important role in the sterol-mediated regulation of SREBP, SCAP and HMG-CoA  reductase. We used PCR-RFLP and DNA sequencing to detect polymorphisms of the  Insig-1 gene in 215 individuals of the Qinchuan cattle breed. Four SNPs  [4366(A>G), 4534(T>C), 5001(T>C), and 5235(G>A)] were indentified.  The association of the genetic viariation with growth and carcass traits (body  length, withers height, hip width, slaughter weight, and carcass weight) was  analyzed. The individuals with better performance had the GG genotype at locus  A4366G, and CC genotypes at locus T4534C and locus T5001C. These could be used  for beef cattle breeding improvement in China. Additionally, linkage  disequilibrium analysis reflected that all mutations were in low linkage  disequilibrium with each other. We concluded that polymorphisms in the Insig-1  gene are associated with growth and carcass traits and could be used for  marker-assisted selection and management in beef cattle breeding programs.
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: X L
authors.initials: XL
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: He
authors.forename: Y L
authors.initials: YL
authors.lastname: Gu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000254:growth & development; D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D015810:Linkage Disequilibrium; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins