title: Prevention of γ-radiation induced cellular genotoxicity by tempol: protection of hematopoietic system.
abstract: Tempol (TPL) under in vitro conditions reduced the extent of gamma radiation induced membrane lipid peroxidation and disappearance of covalently closed circular form of plasmid pBR322. TPL protected cellular DNA from radiation-induced damage in various tissues under ex vivo and in vivo conditions as evidenced by comet assay. TPL also prevented radiation induced micronuclei formation (in peripheral blood leucocytes) and chromosomal aberrations (in bone marrow cells) in whole body irradiated mice. TPL enhanced the rate of repair of cellular DNA (blood leucocytes and bone marrow cells) damage when administered immediately after radiation exposure as revealed from the increased Cellular DNA Repair Index (CRI). The studies thus provided compelling evidence to reveal the effectiveness of TPL to protect hematopoietic system from radiation injury.
authors.affiliation: Amala Cancer Research Centre, Thrissur 680555, Kerala, India.
authors.forename: Lakshmy
authors.initials: L
authors.lastname: Ramachandran
authors.affiliation: Pushpagiri Institute of Medical Sciences & Research Centre, Thiruvalla 689101, Kerala, India. Electronic address: ckknair@yahoo.com.
authors.forename: Cherupally Krishnan Krishnan
authors.initials: CKK
authors.lastname: Nair
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D002869:Chromosome Aberrations / Q000187:drug effects; D003497:Cyclic N-Oxides / Q000494:pharmacology / Q000627:therapeutic use*; D004249:DNA Damage / Q000187:drug effects; D005720:Gamma Rays / Q000009:adverse effects*; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D011837:Radiation-Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D013113:Spin Labels; D013154:Spleen / Q000166:cytology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D003497:Cyclic N-Oxides; D011837:Radiation-Protective Agents; D013113:Spin Labels; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; C001803:tempol