title: Genetic diversity of Setipinna  taty (Engraulidae) populations from the China Sea based on mitochondrial  DNA control region sequences.
abstract: The  genetic diversity of Setipinna taty, which is commercially fished in the  China Sea, was studied based on mitochondrial DNA control region sequences. PCR  was used to amplify the control region fragment in 100 individuals of S.  taty collected from Weihai (WH), Yantai (YT), Zhoushan (ZS), Xiangshan  (XS), and Ninghai (NH) in China. A control region fragment of 656 bp was  successfully sequenced in these 100 individuals. The A+T content of this S.  taty control region was 71.7%; 172 variable sites and 62 haplotypes were  found. Nucleotide diversity in the WH, YT, ZS, XS, and NH groups was 0.0228,  0.0247, 0.0441, 0.0126, and 0.0238, respectively. The haplotype diversity was  0.984, 0.911, 0.989, 0.926, and 0.979, respectively. Analysis of molecular  variance showed that 97.95% of genetic variation was within populations, and  only 2.05% among populations. The neighbor-joining phylogenetic tree obtained  based on genetic distance showed that no significant genealogical structure  exists throughout this range of S. taty. These results indicate no  apparent geographical differentiation in the comparison of Yellow Sea and East  China Sea populations of S. taty. Within the control region, we  identified an extended termination-associated sequence domain, a central  conserved sequence block domain and a conserved sequence block domain;  insertions of short tandem repeat sequence segments were found at the 5' end of  the control region.
authors.affiliation: Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, PR China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Li
authors.forename: T J
authors.initials: TJ
authors.lastname: Xu
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Cheng
authors.forename: D Q
authors.initials: DQ
authors.lastname: Sun
authors.forename: R X
authors.initials: RX
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D014644:Genetic Variation; D006239:Haplotypes; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial