title: Impact of normalization method on experimental outcome using RT-qPCR in Staphylococcus aureus.
abstract: We performed a gene expression study using RT-qPCR in Staphylococcus aureus. The influence of normalization method was investigated. We confirmed that a recent standard, using more reference genes, was the best normalization strategy. The application of the most commonly used reference genes in 2011 (gyrB and 16S rRNA gene) failed.
authors.affiliation: Institute of Chemical Technology, Prague, Faculty of Food and Biochemical Technology, Department of Biochemistry and Microbiology, Prague, Czech Republic. valihral@vscht.cz
authors.forename: Lukas
authors.initials: L
authors.lastname: Valihrach
authors.forename: Katerina
authors.initials: K
authors.lastname: Demnerova
mesh_terms: D027081:DNA Gyrase / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards*; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000592:standards*; D013211:Staphylococcus aureus / Q000235:genetics*; D059467:Transcriptome
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase