title: Production of endothelial cell-enclosing alginate-based hydrogel fibers with a cell adhesive surface through simultaneous cross-linking by horseradish peroxidase-catalyzed reaction in a hydrodynamic spinning process.
abstract: We developed an alginate-based hydrogel fiber enabling to enclose endothelial cells, degradable on-demand by alginate lyase, and having a cell adhesive surface. The hydrogel fiber was obtained by extruding an aqueous solution of 4% (w/v) alginate derivative possessing phenolic hydroxyl moieties (Alg-Ph) and horseradish peroxidase (HRP) into a flow of aqueous solution containing 0.3 mM H(2)O(2) and gelatin derivative possessing Ph moieties (Gelatin-Ph). In the process, cross-linking of Alg-Ph resulting in a hydrogel fiber and immobilization of Gelatin-Ph on the surface of the hydrogel fiber were simultaneously accomplished by an HRP-catalyzed cross-linking reaction between Ph moieties. The diameter of the hydrogel fiber and the quantity of immobilized Gelatin-Ph on the fiber were controllable by changing the flow rates of the solutions and the concentration of HRP in the Alg-Ph-containing solution, respectively. The viability of the human endothelial cells enclosed in the hydrogel fibers obtained by 10 s of flowing in the H(2)O(2)-containing solution was 87.1%. In addition, the cells harvested from the hydrogel fibers through degradation using alginate lyase grew on tissue culture dishes in the same fashion as the cells seeded by a conventional subculture protocol. Human smooth muscle cells adhered, grew and achieved confluence on the surface of the hydrogel fibers. By degrading the hydrogel fibers using alginate lyase, a tubular cell construct was successfully obtained.
authors.affiliation: Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
mesh_terms: D000464:Alginates / Q000737:chemistry* / Q000378:metabolism; D055162:Biocatalysis; D002448:Cell Adhesion; D003432:Cross-Linking Reagents / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D005780:Gelatin / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D011133:Polysaccharide-Lyases / Q000378:metabolism
chemical_list: D000464:Alginates; D003432:Cross-Linking Reagents; D006603:Hexuronic Acids; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid; D005780:Gelatin; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase