title: Gonadectomy differentially regulates steroid receptor coactivator-1 and synaptic proteins in the hippocampus of adult female and male C57BL/6 mice.
abstract: Hippocampus is one of the most important structures that mediates learning and memory, cognition, and mental behaviors and profoundly regulated by sex hormones in a sex-specific manner, but the mechanism of underlying sex differences regulation is still unclear. We have previously reported that in the male and female mice, steroid receptor coactivator-1 (SRC-1) and some key synaptic proteins share similar developmental profile in the hippocampus, but how circulating sex hormones affect hippocampal SRC-1 as well as these synaptic proteins remain unclear. In this study, we examined how gonad sex hormones regulate hippocampal SRC-1, synaptophysin, PSD-95, and AMPA receptor subtype GluR1 by using immunohistochemistry and Western blot. The results showed that in the female mice, ovariectomy affected hippocampal SRC-1 and GluR1 were only detected at 2 weeks post operation, then it recovered to sham level; synaptophysin was unaffected at any timepoint examined; significant decrease of PSD-95 was only detected at 4 weeks post operation. However, in the male hippocampus, SRC-1 and PSD-95 were decreased from one week and lasted to 4 weeks after orchidectomy, GluR1 decreased from 2 weeks after orchidectomy, but synaptophysin remained unchanged as in the females. Correlation analysis showed the profiles of SRC-1 were positively correlated with GluR1 of the females, PSD-95 and GluR1 of the males, respectively. The above results suggested a distinct regulatory mode between female and male gonad hormones in the regulation of hippocampal SRC-1 and synaptic proteins, which may be one of the mechanisms contributing to the dimorphism of hippocampus during development and ageing.
authors.affiliation: Department of Neurobiology, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Bian
authors.forename: Kongjiang
authors.initials: K
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Sen
authors.initials: S
authors.lastname: Lin
authors.forename: Shurong
authors.initials: S
authors.lastname: Li
authors.forename: Bingyin
authors.initials: B
authors.lastname: Su
authors.forename: Jiqiang
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D000076122:Disks Large Homolog 4 Protein; D005260:Female; D012739:Gonadal Steroid Hormones / Q000172:deficiency*; D051528:Guanylate Kinases / Q000032:analysis; D006624:Hippocampus / Q000737:chemistry*; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D056920:Nuclear Receptor Coactivator 1 / Q000032:analysis*; D009919:Orchiectomy; D010052:Ovariectomy; D018091:Receptors, AMPA / Q000032:analysis; D012737:Sex Factors; D013569:Synapses / Q000737:chemistry*; D016708:Synaptophysin / Q000032:analysis
chemical_list: D000076122:Disks Large Homolog 4 Protein; D012739:Gonadal Steroid Hormones; D051528:Guanylate Kinases; D008565:Membrane Proteins; D056920:Nuclear Receptor Coactivator 1; D018091:Receptors, AMPA; D016708:Synaptophysin; C501447:Dlg4 protein, mouse; C569015:Syp protein, mouse; C538861:Ncoa1 protein, mouse