title: Dehalogenation of an anthropogenic compound by an engineered variant of the mouse cytokine macrophage migration inhibitory factor.
abstract: An unconventional dehalogenase: An engineered variant (I64V/V106L) of the mouse cytokine macrophage migration inhibitory factor (MIF) promiscuously catalyzes the hydrolytic dehalogenation of the xenobiotic organohalogen trans-3-chloroacrylic acid to acetaldehyde. Although the dehalogenase activity of this MIF variant is quite low, it achieves an ~10(9) -fold rate enhancement, matching those of conventional enzymes acting on their natural substrates.
authors.affiliation: Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Wasiel
authors.forename: Bert-Jan
authors.initials: BJ
authors.lastname: Baas
authors.forename: Ellen
authors.initials: E
authors.lastname: Zandvoort
authors.forename: Wim J
authors.initials: WJ
authors.lastname: Quax
authors.forename: Gerrit J
authors.initials: GJ
authors.lastname: Poelarends
mesh_terms: D000179:Acrylates / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D054879:Halogenation; D007700:Kinetics; D008263:Macrophage Migration-Inhibitory Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008958:Models, Molecular; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011487:Protein Conformation; D015202:Protein Engineering
chemical_list: D000179:Acrylates; D008263:Macrophage Migration-Inhibitory Factors; D050505:Mutant Proteins; C071773:3-chloroacrylic acid