title: Isolation and differentiation of human cardiomyocyte progenitor cells into cardiomyocytes.
abstract: To date, there is no suitable in vitro model to study human adult cardiac cell biology. Here, we describe a method for efficient isolation and expansion of human cardiomyocyte progenitor cells (CMPCs) from cardiac surgical waste or, alternatively, from fetal heart tissue. Additionally, we provide a detailed in vitro protocol for efficient differentiation of CMPCs into cardiomyocytes with great efficiency (80-90% of differentiation). Once CMPCs are rapidly dividing (approximately 1 month after isolation), differentiation can be achieved in 3-4 weeks.
authors.affiliation: Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Anke M
authors.initials: AM
authors.lastname: Smits
authors.forename: Angelique A
authors.initials: AA
authors.lastname: van Oorschot
authors.forename: Marie-José
authors.initials: MJ
authors.lastname: Goumans
mesh_terms: D002454:Cell Differentiation; D002469:Cell Separation; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*