title: Evaluation of transferability of R-plasmid in bacteriocin-producing donors to bacteriocin-resistant recipients.
abstract: Bacteriocin-producing Escherichia coli (donors) rapidly kill conventional  recipient E. coli DH5α in conjugation experiments. To evaluate plasmid transferability of  bacteriocin-producing donors, we established 2 different bacteriocin-resistant mutants derived  from E. coli DH5α and used them as recipients. When the bacteriocin-resistant mutants were  used in conjugation experiments, the transconjugant recovery from 20 bacteriocin-producing  donors increased from 5% (1/20) to 65% (13/20), and the transfer frequencies increased. These  results showed that bacteriocins inhibited the transfer of the R-plasmid from  bacteriocin-producing donors. Thus, application of bacteriocin-resistant recipients might aid  the evaluation of the potential transferability of plasmids from bacteriocin-producing donors.
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo 185-8511, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Usui
authors.forename: Mototaka
authors.initials: M
authors.lastname: Hiki
authors.forename: Koichi
authors.initials: K
authors.lastname: Murakami
authors.forename: Manao
authors.initials: M
authors.lastname: Ozawa
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Nagai
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Asai
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis* / Q000494:pharmacology*; D003227:Conjugation, Genetic / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D009154:Mutation; D011815:R Factors / Q000235:genetics*
chemical_list: D001430:Bacteriocins; D004269:DNA, Bacterial