title: Isobaric cross-sequence labeling of peptides by using site-selective N-terminus dimethylation.
abstract: Highly site-selective dimethyl labeling of N-terminus of peptides has been obtained by adjusting the acidic strength of the reaction solution. And this selective labeling strategy combined with the use of different isotope formaldehyde reagents has been successfully used in the proteome quantification by using the isobaric peptide cross-sequence labeling method, which would play increasingly important roles in the clinical diagnosis, especially in the discovery of biomarkers for diseases.
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Qin
authors.forename: Fangjun
authors.initials: F
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Hu
authors.forename: Chunxia
authors.initials: C
authors.lastname: Song
authors.forename: Ren'an
authors.initials: R
authors.lastname: Wu
authors.forename: Mingliang
authors.initials: M
authors.lastname: Ye
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D000588:Amines / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry; D008745:Methylation; D010455:Peptides / Q000737:chemistry*; D020543:Proteome / Q000737:chemistry; D013194:Staining and Labeling
chemical_list: D000588:Amines; D010455:Peptides; D020543:Proteome; D005557:Formaldehyde