title: Septic shock and chemotherapy-induced cytopenia: effects on microcirculation.
abstract: OBJECTIVE
  Neutrophil and platelet activation and their interactions with endothelial cells are considered central features of sepsis-induced microcirculatory alterations. However, no study has evaluated the microvascular pattern of septic shock patients with chemotherapy-induced severe cytopenia.
  
  
  METHODS
  Demographic and hemodynamic variables together with sublingual microcirculation recording [orthogonal polarization spectral imaging enhanced by sidestream dark-field technology (OPS-SDF) videomicroscopy] were collected in four groups of subjects: septic shock (SS, N = 9), septic shock in cytopenic patients (NSS, N = 8), cytopenia without infection (NEUTR, N = 7), and healthy controls (CTRL, N = 13). Except for controls, all measurements were repeated after complete resolution of septic shock and/or neutropenia. Video files were processed using appropriate software tool and semiquantitatively evaluated [total vascular density (TVD, mm/mm(2)), perfused vessel density (PVD, mm/mm(2)), proportion of perfused vessels (PPV, %), mean flow index (MFI), and flow heterogeneity index (FHI)].
  
  
  RESULTS
  Compared with controls, there were statistically significant microcirculatory alterations within all tested groups of patients (TVD: SS = 8.8, NSS = 8.8, NEUTR = 9.1 versus CTRL = 12.6, p < 0.001; PVD: SS = 6.3, NSS = 6.1, NEUTR = 6.9 versus CTRL = 12.5, p < 0.001; PPV: SS = 71.6, NSS = 68.9, NEUTR = 73.3 versus CTRL = 98.7, p < 0.001; MFI: SS = 2.1, NSS = 1.9, NEUTR = 2.1 versus CTRL = 3.0, p < 0.05; FHI: SS = 1.0, NSS = 0.9, NEUTR = 0.6 versus CTRL = 0.0, p < 0.001). No significant differences were detected between SS, NSS, and NEUTR groups at baseline. Incomplete restoration of microcirculatory perfusion was observed after septic shock and/or neutropenia resolution with a trend towards better recovery in MFI and FHI variables in NSS as compared with SS patients.
  
  
  CONCLUSIONS
  Microvascular derangements in septic shock did not differ between noncytopenic and cytopenic patients. Our data might suggest that profound neutropenia and thrombocytopenia do not render microcirculation more resistant to sepsis-induced microvascular alterations. The role and mechanisms of microvascular alterations associated with chemotherapy-induced cytopenia warrant further investigation.
authors.affiliation: 1st Department of Medicine, Intensive Care Unit, Charles University in Prague, Medical School and Teaching Hospital in Pilsen, Alej Svobody 80, 304 60 Pilsen, Czech Republic.
authors.forename: Thomas
authors.initials: T
authors.lastname: Karvunidis
authors.forename: Jiri
authors.initials: J
authors.lastname: Chvojka
authors.forename: Daniel
authors.initials: D
authors.lastname: Lysak
authors.forename: Roman
authors.initials: R
authors.lastname: Sykora
authors.forename: Ales
authors.initials: A
authors.lastname: Krouzecky
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Radej
authors.forename: Ivan
authors.initials: I
authors.lastname: Novak
authors.forename: Martin
authors.initials: M
authors.lastname: Matejovic
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000009:adverse effects*; D001808:Blood Vessels / Q000502:physiology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008833:Microcirculation / Q000502:physiology*; D018715:Microscopy, Video; D008875:Middle Aged; D009060:Mouth Floor / Q000098:blood supply; D009061:Mouth Mucosa / Q000098:blood supply; D009503:Neutropenia / Q000139:chemically induced / Q000503:physiopathology*; D010198:Pancytopenia / Q000139:chemically induced / Q000503:physiopathology*; D012772:Shock, Septic / Q000503:physiopathology*
chemical_list: D000970:Antineoplastic Agents