title: Molecular genetic characterization of lymphoma: application to cytology diagnosis.
abstract: Mature B- and T-cell lymphomas are increasingly being associated with specific genetic alterations; characterization of these changes can sometimes be crucial to both diagnosis and prognosis. Molecular testing encompasses fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR)-based assays, as well as classical cytogenetics. FISH and PCR-based assays can be performed on cytology material without the need for advanced planning for testing with fresh tissue. As many lymphoproliferations are amenable to fine needle aspiration diagnosis, it is important for the cytopathologist to be aware of what testing is feasible in these (sometimes limited) specimens. Here, we review pertinent genetic changes in mature B- and T-cell lymphomas with a focus on those that may be encountered more commonly in cytology specimens.
authors.affiliation: Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Rachel C
authors.initials: RC
authors.lastname: Ochs
authors.forename: Adam
authors.initials: A
authors.lastname: Bagg
mesh_terms: D049109:Cell Proliferation; D002877:Chromosomes, Human / Q000235:genetics; D003581:Cytodiagnosis / Q000379:methods*; D004268:DNA-Binding Proteins / Q000235:genetics; D055088:Early Detection of Cancer / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D051560:Proto-Oncogene Proteins c-bcl-6; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012680:Sensitivity and Specificity; D013964:Thyroid Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D014178:Translocation, Genetic
chemical_list: C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; C489427:MYC protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051560:Proto-Oncogene Proteins c-bcl-6; D016271:Proto-Oncogene Proteins c-myc