title: [Two spreading states of mesenchymal cells of the human embryo in vitro].
abstract: Spreading mesenchymal cells of human embryo on plastic and type I collagens (from rat, sheep and bull) was studied. Spreading of the cells on collagens was stronger than that in the control but no differences between the different collagens were revealed. The cell perimeter, the spreading coefficient and the cell projection area on the substrate were used as morphometric parametres. The spreading of cells was monitored for 0.5-2 h after plating. During the spreading both on plastic and on collagen, the groups of small cells were revealed as separate subpopulations. As a whole, such cells comprehend 9 % of the cell population in the control and 2% in experiment. We assume that this cell type is associated with a special independent functional state of the cells that precedes cell spreading.
authors.forename: T A
authors.initials: TA
authors.lastname: Krylova
authors.forename: L V
authors.initials: LV
authors.lastname: Kukhareva
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Petrov
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010969:Plastics / Q000737:chemistry; D011247:Pregnancy; D051381:Rats; D012756:Sheep; D013045:Species Specificity
chemical_list: D024042:Collagen Type I; D010969:Plastics