title: In vivo preclinical photoacoustic imaging of tumor vasculature development and therapy.
abstract: The use of a novel all-optical photoacoustic scanner for imaging the development of tumor vasculature and its response to a therapeutic vascular disrupting agent is described. The scanner employs a Fabry-Perot polymer film ultrasound sensor for mapping the photoacoustic waves and an image reconstruction algorithm based upon attenuation-compensated acoustic time reversal. The system was used to noninvasively image human colorectal tumor xenografts implanted subcutaneously in mice. Label-free three-dimensional in vivo images of whole tumors to depths of almost 10 mm with sub-100-micron spatial resolution were acquired in a longitudinal manner. This enabled the development of tumor-related vascular features, such as vessel tortuosity, feeding vessel recruitment, and necrosis to be visualized over time. The system was also used to study the temporal evolution of the response of the tumor vasculature following the administration of a therapeutic vascular disrupting agent (OXi4503). This revealed the well-known destruction and recovery phases associated with this agent. These studies illustrate the broader potential of this technology as an imaging tool for the preclinical and clinical study of tumors and other pathologies characterized by changes in the vasculature.
authors.affiliation: University College London, Department of Medical Physics and Bioengineering, Gower Street, London WC1E 6BT, United Kingdom. jan.laufer@charite.de
authors.forename: Jan
authors.initials: J
authors.lastname: Laufer
authors.forename: Peter
authors.initials: P
authors.lastname: Johnson
authors.forename: Edward
authors.initials: E
authors.lastname: Zhang
authors.forename: Bradley
authors.initials: B
authors.lastname: Treeby
authors.forename: Ben
authors.initials: B
authors.lastname: Cox
authors.forename: Barbara
authors.initials: B
authors.lastname: Pedley
authors.forename: Paul
authors.initials: P
authors.lastname: Beard
mesh_terms: D020533:Angiogenesis Inhibitors / Q000627:therapeutic use; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000098:blood supply / Q000000981:diagnostic imaging* / Q000188:drug therapy*; D011756:Diphosphates / Q000627:therapeutic use*; D054459:Elasticity Imaging Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging* / Q000188:drug therapy*; D061088:Photoacoustic Techniques / Q000379:methods*; D013267:Stilbenes / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents; D011756:Diphosphates; C465018:Oxi 4503; D013267:Stilbenes