title: Avian influenza H9N2 seroprevalence among poultry workers in Pune, India, 2010.
abstract: Avian influenza (AI) H9N2 has been reported from poultry in India. A seroepidemiological study was undertaken among poultry workers to understand the prevalence of antibodies against AI H9N2 in Pune, Maharashtra, India. A total of 338 poultry workers were sampled. Serum samples were tested for presence of antibodies against AI H9N2 virus by hemagglutination inhibition (HI) and microneutralization (MN) assays. A total of 249 baseline sera from general population from Pune were tested for antibodies against AI H9N2 and were negative by HI assay using ≥40 cut-off antibody titre. Overall 21 subjects (21/338 = 6.2%) were positive for antibodies against AI H9N2 by either HI or MN assays using ≥40 cut-off antibody titre. A total of 4.7% and 3.8% poultry workers were positive for antibodies against AI H9N2 by HI and MN assay respectively using 40 as cut-off antibody titre. This is the first report of seroprevalence of antibodies against AI H9N2 among poultry workers in India.
authors.affiliation: National Institute of Virology, Microbial Containment Complex, Pashan, Pune, India.
authors.forename: Shailesh D
authors.initials: SD
authors.lastname: Pawar
authors.forename: Babasaheb V
authors.initials: BV
authors.lastname: Tandale
authors.forename: Chandrashekhar G
authors.initials: CG
authors.lastname: Raut
authors.forename: Saurabh S
authors.initials: SS
authors.lastname: Parkhi
authors.forename: Tanaji D
authors.initials: TD
authors.lastname: Barde
authors.forename: Yogesh K
authors.initials: YK
authors.lastname: Gurav
authors.forename: Sadhana S
authors.initials: SS
authors.lastname: Kode
authors.forename: Akhilesh C
authors.initials: AC
authors.lastname: Mishra
mesh_terms: D000382:Agricultural Workers' Diseases / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007194:India / Q000453:epidemiology; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology*; D005585:Influenza in Birds / Q000453:epidemiology* / Q000276:immunology / Q000635:transmission; D007251:Influenza, Human / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D009500:Neutralization Tests; D016273:Occupational Exposure; D011200:Poultry; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral