title: An LC method for the analysis of phosphatidylcholine hydrolysis products and its application to the monitoring of the acyl migration process.
abstract: An assay for quantitative analysis of phosphatidylcholine (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) and its hydrolysis products: 1-hydroxy-2-palmitoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine, sn-glycero-3-phosphocholine and palmitic acid using high-performance liquid chromatography with charge aerosol detector (CAD) was developed. The separation of the compounds of interest was achieved on a reversed-phase/hydrophilic interaction stationary phase with a mobile phase consisting of acetonitrile:methanol:10mM ammonium acetate solution. The method was applied to control the acyl migration process of LPC regioisomers in the most common solvents used in the synthesis or modification of PC.
authors.affiliation: Department of Chemistry, Wrocław University of Environmental and Life Sciences, Norwida 25 50-375 Wrocław, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Kiełbowicz
authors.forename: Damian
authors.initials: D
authors.lastname: Smuga
authors.forename: Witold
authors.initials: W
authors.lastname: Gładkowski
authors.forename: Anna
authors.initials: A
authors.lastname: Chojnacka
authors.forename: Czesław
authors.initials: C
authors.lastname: Wawrzeńczyk
mesh_terms: D000097:Acetonitriles; D000336:Aerosols; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D006868:Hydrolysis; D008244:Lysophosphatidylcholines / Q000032:analysis*; D000432:Methanol; D019308:Palmitic Acid / Q000032:analysis*; D010713:Phosphatidylcholines / Q000032:analysis*; D013237:Stereoisomerism
chemical_list: D000097:Acetonitriles; D000336:Aerosols; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D019308:Palmitic Acid; D000432:Methanol; C032159:acetonitrile