title: An explant based-method for differentiating adipocytes from equine adipose tissue.
abstract: Culturing adipocytes enables fine control of experimental conditions and helps minimise animal use. This report describes an explant-based method for isolating stromal-vascular cells from equine adipose tissue that enables use of small amounts of tissue. Subcutaneous and mesenteric adipose tissues were harvested post mortem and stromal-vascular cells grown from explants, prior to testing the capacity of several differentiation media to induce lipid droplet formation and increase transcript abundance of adipocyte markers. Inclusion of rosiglitazone at 1 and 5 µmol/l concentrations, along with other media components, induced differentiation of cultured equine stromal-vascular cells derived from subcutaneous and mesenteric adipose tissues.
authors.affiliation: Department of Dairy Science, Virginia Tech, Blacksburg, Virginia, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Suagee
authors.forename: B A
authors.initials: BA
authors.lastname: Corl
mesh_terms: D017667:Adipocytes / Q000145:classification*; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D006736:Horses / Q000502:physiology*; D000077154:Rosiglitazone; D017154:Stromal Cells; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D045162:Thiazolidinediones; D000077154:Rosiglitazone