title: [Measurement of purines and uric acid simultaneous in meat with high performance liquid chromatographys].
abstract: OBJECTIVE
  To determinate adenine, guanine, hypoxanthine, xanthine and uric acid simultaneously in meat, a reversed-phase high performance liquid chromatography (HPLC) was developed.
  
  
  METHODS
  The meat were hydrolyzed with perchloric acid 10% (v/v) in boiling water for 60 mm. After the hydrolysate was adjusted to pH 4, centrifuge , and filtrated with a 0.45 mirom membrane, the supernatants were separated on an Agilent ZORBAX Eclipse XDB-C18 column (250 mm x 4.6 mm i.d., 5 microm) at 25 degrees C with a mobile phase of 7 x 10(-3) mol/L KH2PO4-H3P04(pH 4.0) ,a flow rate of 1.0 ml/ mm, and UV detection at 254 nm.
  
  
  RESULTS
  Each component in the corresponding concentration range showed a good linear relation with its peak area, correlation coefficient r > 0.9999, recovery was 90.0%-107. 5%, RSD was 1.7%-13.3%. In addition to containing four kinds of purines, there was quite amount of uric acid (about 133.7 -86.2 pug/g) in the mentioned meat. The ratio of uric acid to total purine and uric acid was about 7%. The content of total purine in chicken was (1759.3 +/- 64.6) microg/g higher than in rabbit, mutton, pork and beef (1440-1000 microg/g).
  
  
  CONCLUSIONS
  The validated method is simple, rapid, accurate and reliable to the determination of purines and uric acid in meat.
authors.affiliation: Department of Sanitary Chemistry, School of Public Health, Shanxi Medical University, Taiyuan 030001, China. yanghb1984@sina.com
authors.forename: Haibin
authors.initials: H
authors.lastname: Yang
authors.forename: Jialing
authors.initials: J
authors.lastname: Zhang
authors.forename: Guiying
authors.initials: G
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D008460:Meat / Q000032:analysis*; D011687:Purines / Q000032:analysis*; D011817:Rabbits; D012756:Sheep; D013552:Swine; D014527:Uric Acid / Q000032:analysis*
chemical_list: D011687:Purines; D014527:Uric Acid