title: Antigen-capture enzyme-linked immunosorbent assays for detection of different H5 avian Influenza A virus.
abstract: Avian Influenza A virus (AIV) subtype H5 is divided into American and Eurasian lineages, according to hemagglutinin gene sequences. Although methods for detecting H5 AIVs have been described, no H5 strain-specific detection method has been reported. The purpose of the present study was to develop an antigen-capture enzyme-linked immunosorbent assay (ACE) to detect and differentiate between the American and the Eurasian H5 AIVs. Monoclonal antibodies (mAbs) against the HA fragment of a Eurasian H5N2 AIV were used as the capture antibodies as well as the detector antibodies after labeling with horseradish peroxidase to develop an ACE. One mAb was selected for detecting the American as well as the Eurasian H5 AIVs. The other mAb was used for detecting only the Eurasian H5N2 but not the American H5N2 AIVs, H6N1 AIVs, or Newcastle disease virus. The ACEs developed would be useful for detection and differentiation of H5 AIVs from the Eurasian and the American H5 AIVs.
authors.affiliation: School of Veterinary Medicine, National Taiwan University, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Yi-Chung
authors.initials: YC
authors.lastname: Chiu
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Chu
authors.forename: Zak
authors.initials: Z
authors.lastname: Tsao
authors.forename: Ching-Ho
authors.initials: CH
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D053125:Influenza A Virus, H5N2 Subtype / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D057230:Limit of Detection; D013047:Specific Pathogen-Free Organisms
chemical_list: D000911:Antibodies, Monoclonal; D019267:Hemagglutinin Glycoproteins, Influenza Virus