title: Infiltration of colorectal carcinoma by S100+ dendritic cells and CD57+ lymphocytes as independent prognostic factors after radical surgical treatment.
abstract: BACKGROUND
  S100(+) dendritic cells and CD57(+) lymphocytes are factors reflecting the immune system's ability to suppress the progress of tumor growth. CD57(+) cells include natural killer cells and late stages of T-effector lymphocytes. We evaluated the relationship between the known clinical and histological factors and tumor markers as well as the presence of S100(+) and CD57(+) cells in the tissue of colorectal carcinoma with the aim of detecting patients at high risk of short overall survival (OS) or short disease-free interval (DFI) after radical surgical treatment and we further analyzed whether S100(+) and CD57(+) positivity could bring on new information regarding the treatment regimen.
  
  
  METHODS
  Data of 150 patients (97 males and 53 females) that underwent an elective radical surgical procedure for colorectal cancer were studied. The influence on DFI and on OS of the following parameters was evaluated: grading, staging and positivity for S100 and CD57 by immunohistochemical staining. We also analyzed the relation of preoperative serum levels of the tumor markers Carcinoembryonic Antigen (CEA), Cancer Antigen 19-9 (CA19-9), Cancer Antigen 72-4 (CA72-4), Thymidine kinase (TK), Tissue-Specific Polypeptide Antigen (TPS) and Tissue Polypeptide Antigen (TPA) in relation to S100 and CD57 positivity/negativity for the same patients.
  
  
  RESULTS
  OS at 1, 3 and 5 years was 92.2%, 76.5% and 70.2%; the DFI at 1, 3 and 5 years was 85.3%, 64.3% and 49.4%. CD57 positivity in the tumor mass was proven as a positive prognostic factor for OS. Risk of short OS was 2.5-fold higher in patients with low tumor infiltration by CD57(+) lymphocytes. The combination of N2 stage for lymph nodes and the absence of CD57(+) cells was proven to be the strongest negative prognostic factor for OS. No significant influence of CD57 positivity on DFI appeared. There was no significant influence of S100 positivity on OS or DFI; nor was there any statistical dependence of CD57 and S100 positivity or negativity on preoperative serum levels of CEA, CA19-9, CA72-4, TK, TPS or TPA. Both studied factors were shown to be statistically independent factors.
  
  
  CONCLUSIONS
  The present study showed infiltration of colorectal cancer tissue by CD57(+) cells as being an important independent positive prognostic factor for OS.
authors.affiliation: Department of Surgery, Medical School and Teaching Hospital Pilsen, Charles University in Prague, Alej Svobody 80, 304 00 Pilsen, Czech Republic. LiskaV@fnplzen.cz
authors.forename: Vaclav
authors.initials: V
authors.lastname: Liska
authors.forename: Ondrej
authors.initials: O
authors.lastname: Vycital
authors.forename: Ondrej
authors.initials: O
authors.lastname: Daum
authors.forename: Petr
authors.initials: P
authors.lastname: Novak
authors.forename: Vladislav
authors.initials: V
authors.lastname: Treska
authors.forename: Jan
authors.initials: J
authors.lastname: Bruha
authors.forename: Pavel
authors.initials: P
authors.lastname: Pitule
authors.forename: Lubos
authors.initials: L
authors.lastname: Holubec
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D018959:CD57 Antigens / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D011379:Prognosis; D009418:S100 Proteins / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D014408:Biomarkers, Tumor; D018959:CD57 Antigens; D009418:S100 Proteins