title: Saccharification of woody biomass using glycoside hydrolases from Stereum hirsutum.
abstract: Enzymatic saccharification of woody biomasses was performed using glycoside hydrolases from Stereum hirsutum, a newly isolated fungal strain found to secrete efficient glycoside hydrolases. The strain showed the highest β-glucosidase, cellobiohydrolase, endoglucanase, endoxylanase, laccase, and filter paper activity of 10.3, 1.7, 10.3, 29.9, 0.12, and 0.58 U/ml, respectively. Among the various biomasses tested for saccharification, pine biomass produced maximum reducing sugar. Response surface methodology was used to optimize the hydrolysis of pine biomass to achieve the highest level of sugars. The parameters including enzyme, substrate concentration, temperature and pH were found to be critical for the conversion of pine biomass into sugars. Maximum saccharification of 49.7% (435 mg/g-substrate) was obtained after 96 h of hydrolysis. A close agreement between the experimental results and the model predictions was achieved. S. hirsutum could be a good choice for the production of reducing sugars from cellulosic biomasses.
authors.affiliation: Institute of SK-KU Biomaterials, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Marimuthu
authors.initials: M
authors.lastname: Jeya
authors.forename: Dayanand
authors.initials: D
authors.lastname: Kalyani
authors.forename: Saurabh Sudha
authors.initials: SS
authors.lastname: Dhiman
authors.forename: Hoon
authors.initials: H
authors.lastname: Kim
authors.forename: Seongmin
authors.initials: S
authors.lastname: Woo
authors.forename: Dongwook
authors.initials: D
authors.lastname: Kim
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
mesh_terms: D001487:Basidiomycota / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D042845:Laccase / Q000096:biosynthesis; D008954:Models, Biological; D009584:Nitrogen / Q000494:pharmacology; D010802:Phylogeny; D028223:Pinus / Q000187:drug effects / Q000378:metabolism; D012044:Regression Analysis; D015203:Reproducibility of Results; D013379:Substrate Specificity / Q000187:drug effects; D013501:Surface-Active Agents / Q000494:pharmacology; D013997:Time Factors; D014934:Wood / Q000187:drug effects / Q000378:metabolism*
chemical_list: D013501:Surface-Active Agents; D042845:Laccase; D006026:Glycoside Hydrolases; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; D009584:Nitrogen