title: Expression of complete metabolic pathways in transgenic plants.
abstract: Plant genetic engineering emerged as a methodology to introduce only few transgenes into the plant genome. Following fast-paced developments of the past few decades, engineering of much larger numbers of transgenes became a reality, allowing to introduce full metabolic pathways from other organisms into plants and generate transgenics with startling new traits. From the advent of the classical plant genetic engineering, the transgenes were introduced into the nuclear genome of the plant cell, and this strategy still is quite successful when applied to few transgenes. However, for introducing large number of transgenes, we advocate that the chloroplast genome is a superior choice, especially for engineering of new complete metabolic pathways into plants. The ability to genetically engineer plants with complex and fully functional metabolic pathways from other organisms bears a substantial promise in generation of pharmaceuticals, i.e., biopharming, and new agricultural crops with that traits never existed before, leading to enhancement in quality of human life.
authors.affiliation: Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794, USA. vitaly.citovsky@stonybrook.edu
authors.forename: Alexander
authors.initials: A
authors.lastname: Krichevsky
authors.forename: Adi
authors.initials: A
authors.lastname: Zaltsman
authors.forename: Lisa
authors.initials: L
authors.lastname: King
authors.forename: Vitaly
authors.initials: V
authors.lastname: Citovsky
mesh_terms: D001709:Biotechnology / Q000379:methods; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism; D018556:Crops, Agricultural / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D059828:Plant Cells / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic / Q000235:genetics; D019076:Transgenes / Q000235:genetics