title: T cell-specific siRNA delivery using antibody-conjugated chitosan nanoparticles.
abstract: The intracellular delivery of small interfering RNA (siRNA) plays a key role in RNA interference (RNAi) and provides an emerging technique to treat various diseases, including infectious diseases. Chitosan has frequently been used in gene delivery applications, including siRNA delivery. However, studies regarding the modification of chitosan with antibodies specifically targeting T cells are lacking. We hypothesized that chitosan nanoparticles modified with T cell-specific antibodies would be useful for delivering siRNA to T cells. CD7-specific single-chain antibody (scFvCD7) was chemically conjugated to chitosan by carbodiimide chemistry, and nanoparticles were prepared by a complex coacervation method in the presence of siRNA. The mean diameter and zeta potential of the scFvCD7-chitosan/siRNA nanoparticles were approximately 320 nm and +17 mV, respectively, and were not significantly influenced by the coupling of antibody to chitosan. The cellular association of antibody-conjugated nanoparticles to CD4+ T cell lines as well as gene silencing efficiency in the cells was significantly improved compared to nonmodified chitosan nanoparticles. This approach to introducing T cell-specific antibody to chitosan nanoparticles may find useful applications for the treatment of various infectious diseases.
authors.affiliation: Department of Bioengineering and Institute for Bioengineering and Biopharmaceutical Research, Hanyang University , Seoul 133-791, Republic of Korea.
authors.forename: Jangwook
authors.initials: J
authors.lastname: Lee
authors.forename: Kyoung-Soo
authors.initials: KS
authors.lastname: Yun
authors.forename: Chang Seon
authors.initials: CS
authors.lastname: Choi
authors.forename: Seung-Hwa
authors.initials: SH
authors.lastname: Shin
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Ban
authors.forename: Taiyoun
authors.initials: T
authors.lastname: Rhim
authors.forename: Sang Kyung
authors.initials: SK
authors.lastname: Lee
authors.forename: Kuen Yong
authors.initials: KY
authors.lastname: Lee
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D019011:Antigens, CD7 / Q000276:immunology; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems; D006801:Humans; D019169:Jurkat Cells; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
chemical_list: D055910:Antibodies, Immobilized; D019011:Antigens, CD7; D034741:RNA, Small Interfering; D048271:Chitosan