title: Proteomic analysis of the microenvironment of developing oocytes.
abstract: We utilized a setup based on extensive pre-fractionation of proteolytic peptides and nanoflow reversed-phase LC-MS/MS to identify the (sub)proteome of human follicular fluid (FF). In this in-depth screen, 246 specific proteins were identified, the majority of which are involved in coagulation- and immune-response pathways. Our aim is to define a set of FF protein markers, which could predict oocyte quality.
authors.affiliation: Department of Obstetrics and Gynecology, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: John
authors.initials: J
authors.lastname: Twigt
authors.forename: Regine P
authors.initials: RP
authors.lastname: Steegers-Theunissen
authors.forename: Karel
authors.initials: K
authors.lastname: Bezstarosti
authors.forename: Jeroen A A
authors.initials: JA
authors.lastname: Demmers
mesh_terms: D060833:Cellular Microenvironment / Q000502:physiology*; D056148:Chromatography, Reverse-Phase; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007525:Isoelectric Focusing; D009865:Oocytes / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: D010446:Peptide Fragments; D020543:Proteome