title: Preparation of a pH-sensitive polyacrylate amphiphilic copolymer and its application in cellulase immobilization.
abstract: P(MDB), a pH-sensitive and reversible water-soluble copolymer, was synthesized with methacrylic acid (MAA), 2-(dimethylamino) ethyl methacrylate (DMAEMA), and butyl methacrylate (BMA) and used as carrier for cellulase. The copolymer is insoluble between pH 2.5 and 4.1, and soluble below pH 2.5 or above 4.1. Its recovery in aqueous solution was 97.2% by adjusting its isoelectric point (pI) to 3.1. Cellulase was covalently immobilized on P(MDB) with 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide. Under optimized conditions, the activity yield of immobilized cellulase was 63.24% and its recovery was 96.8% by adjusting the pI to 3.5. Maximum activity of the immobilized cellulase was achieved at 60 °C (pH 5.0), while free cellulase exhibited maximum activity at 55 °C (pH 5.0). The immobilized cellulase retained 83.1% of its initial activity after repeated five cycles of hydrolysis reaction. P(MDB) is a promising carrier for immobilizing enzymes with high and low optimum pH due to its dissolving characteristics.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Liang
authors.forename: Xuejun
authors.initials: X
authors.lastname: Cao
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis*; D001709:Biotechnology / Q000379:methods*; D002234:Carbodiimides; D002480:Cellulase / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D009994:Osmolar Concentration; D011108:Polymers / Q000138:chemical synthesis*; D059027:Recycling; D012995:Solubility; D013379:Substrate Specificity; D013501:Surface-Active Agents / Q000138:chemical synthesis*; D013696:Temperature; D013997:Time Factors; D014866:Waste Products / Q000032:analysis; D003313:Zea mays / Q000737:chemistry
chemical_list: C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D000180:Acrylic Resins; D002234:Carbodiimides; D004800:Enzymes, Immobilized; D011108:Polymers; D013501:Surface-Active Agents; D014866:Waste Products; C006903:carbopol 940; D002480:Cellulase