title: Supercooled aqueous nuclear magnetic resonance using agarose gels.
abstract: Low-temperature nuclear magnetic resonance (NMR), especially under supercooled conditions, can give critical insight into biomolecular systems via slowed dynamics and exchange rates. These conditions can also increase correlation times of small molecules, potentially allowing for NMR structural study of small molecules at moderate field strengths. Agarose gels allow for supercooled conditions and are simple to prepare, invisible to NMR, and noninteractive with most biomolecules and organics. Here we demonstrate their use with nucleic acids, small organic molecules, and peptides.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Spring
authors.forename: Markus W
authors.initials: MW
authors.lastname: Germann
mesh_terms: D003080:Cold Temperature; D004247:DNA / Q000032:analysis*; D005782:Gels / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D012685:Sepharose / Q000737:chemistry*; D013395:Sucrose / Q000032:analysis*; D014867:Water / Q000737:chemistry
chemical_list: D005782:Gels; D009841:Oligonucleotides; D010455:Peptides; D014867:Water; D013395:Sucrose; D004247:DNA; D012685:Sepharose