title: Study of drug metabolism by xanthine oxidase.
abstract: In this work, we report the studies of drug metabolism by xanthine oxidase (XOD) with electrochemical techniques. Firstly, a pair of stable, well-defined and quasi-reversible oxidation/reduction peaks is obtained with the formal potential at -413.1 mV (vs. SCE) after embedding XOD in salmon sperm DNA membrane on the surface of pyrolytic graphite electrode. Then, a new steady peak can be observed at -730 mV (vs. SCE) upon the addition of 6-mercaptopurine (6-MP) to the electrochemical system, indicating the metabolism of 6-MP by XOD. Furthermore, the chronoamperometric response shows that the current of the catalytic peak located at -730 mV increases with addition of 6-MP in a concentration-dependent manner, and the increase of the chronoamperometric current can be inhibited by an XOD inhibitor, quercetin. Therefore, our results prove that XOD/DNA modified electrode can be efficiently used to study the metabolism of 6-MP, which may provide a convenient approach for in vitro studies on enzyme-catalyzed drug metabolism.
authors.affiliation: Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Obstetrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210036, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Obstetrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210036, China.
authors.forename: Lizhou
authors.initials: L
authors.lastname: Sun
authors.affiliation: Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China.|Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
authors.forename: Genxi
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000378:metabolism*; D002384:Catalysis; D004247:DNA / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism*; D008297:Male; D015122:Mercaptopurine / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011794:Quercetin / Q000494:pharmacology; D020317:Salmo salar; D013094:Spermatozoa / Q000166:cytology; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000964:Antimetabolites, Antineoplastic; D004800:Enzymes, Immobilized; D004247:DNA; D011794:Quercetin; D015122:Mercaptopurine; D014969:Xanthine Oxidase