title: Blood basophils from cystic fibrosis patients with allergic bronchopulmonary aspergillosis are primed and hyper-responsive to stimulation by aspergillus allergens.
abstract: BACKGROUND
  Fifteen to sixty percent of cystic fibrosis patients harbor Aspergillus fumigatus (Af) in their airways (CF-AC) and some will develop allergic bronchopulmonary aspergillosis (CF-ABPA). Since basophils play a key role in allergy, we hypothesized that they would display alterations in CF-ABPA patients compared to CF-AC or patients without Af colonization (CF).
  
  
  METHODS
  Using flow cytometry, we measured CD203c, CD63 and CD123 levels on basophils from CF-ABPA (N=11), CF-AC (N=14), and CF (N=12) patients before and after ex vivo stimulation with Af allergens.
  
  
  RESULTS
  Baseline CD203c was increased in basophils from CF-ABPA compared to CF-AC and CF patients. Af extract and recombinant Aspf1 stimulated basophils from CF-ABPA patients to markedly upregulate CD203c, along with modest upregulation of CD63 and a CD123 downward trend. Plasma TARC/CCL17 at baseline and post-stimulation cell supernatant histamine levels were similar in the three groups.
  
  
  CONCLUSIONS
  In CF-ABPA, blood basophils are primed and hyperresponsive to Af allergen stimulation.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA. yaelgernez@yahoo.fr
authors.forename: Yael
authors.initials: Y
authors.lastname: Gernez
authors.forename: Colleen E
authors.initials: CE
authors.lastname: Dunn
authors.forename: Cassie
authors.initials: C
authors.lastname: Everson
authors.forename: Erin
authors.initials: E
authors.lastname: Mitsunaga
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Gudiputi
authors.forename: Karolina
authors.initials: K
authors.lastname: Krasinska
authors.forename: Zoe A
authors.initials: ZA
authors.lastname: Davies
authors.forename: Leonore A
authors.initials: LA
authors.lastname: Herzenberg
authors.forename: Rabindra
authors.initials: R
authors.lastname: Tirouvanziam
authors.forename: Richard B
authors.initials: RB
authors.lastname: Moss
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology / Q000494:pharmacology; D000946:Antigens, Fungal / Q000276:immunology; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000276:immunology*; D001232:Aspergillus fumigatus / Q000276:immunology*; D001491:Basophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D015550:Cell Degranulation / Q000276:immunology; D054414:Chemokine CCL17 / Q000097:blood; D002648:Child; D003550:Cystic Fibrosis / Q000276:immunology* / Q000382:microbiology; D005260:Female; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D053650:Interleukin-3 Receptor alpha Subunit / Q000378:metabolism; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011755:Pyrophosphatases / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology; D060149:Tetraspanin 30 / Q000378:metabolism; D055815:Young Adult
chemical_list: C081947:ASPF1 protein, Aspergillus fumigatus; D000485:Allergens; D000946:Antigens, Fungal; C106230:CCL17 protein, human; C554586:CD63 protein, human; D054414:Chemokine CCL17; C427944:ENPP3 protein, human; D005656:Fungal Proteins; C115022:IL3RA protein, human; D053650:Interleukin-3 Receptor alpha Subunit; D011994:Recombinant Proteins; D060149:Tetraspanin 30; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases