title: Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit.
abstract: A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC-dUTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the theoretic limit.
authors.affiliation: Department of Chemical and Biomolecular Engineering, HKUST, Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.forename: Jianzhao
authors.initials: J
authors.lastname: Liu
authors.forename: Zujin
authors.initials: Z
authors.lastname: Zhao
authors.forename: Ka Ming
authors.initials: KM
authors.lastname: Ng
authors.forename: Kathy Qian
authors.initials: KQ
authors.lastname: Luo
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang