title: In vitro oxidant effects of D-glucosamine reduce adhesión and biofilm formation of Staphylococcus epidermidis.
abstract: Staphylococcus epidermidis is a common pathogen in medical device-associated infections. Its major pathogenic factor is the ability to form adherent biofilms. In this work, three S. epidermidis strains isolated from infected catheters were chosen with the objective of investigating the effect of D-glucosamine (D-Glu) on reactive oxygen species (ROS) production, adhesion and biofilm formation. The chemiluminescence and nitroblue tetrazolium reduction assays were used to determine ROS production by planktonic S. epidermidis and the microtiter plate assay to quantify in vitro biofilm formation. D-Glu generated a dose-dependent increase in ROS in planktonic cells with maximum stimuli at a concentration of 0.05 mM, and reduced adhesion and biofilm formation. On the other hand, glucose showed an antioxidative stress action and promoted biofilm adhesion and growth. This study suggests a potential application of D-Glu against infections associated with indwelling medical devices, since the oxidative stress caused by this hexosamine in planktonic S. epidermidis contributed to reducing biofilm formation.
authors.affiliation: Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba-Ciudad Universitaria - Haya de la Torre y Medina Allende, Argentina. aiassa@fcq.unc.edu.ar
authors.forename: Virginia
authors.initials: V
authors.lastname: Aiassa
authors.forename: Ana I
authors.initials: AI
authors.lastname: Barnes
authors.forename: Inés
authors.initials: I
authors.lastname: Albesa
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D057785:Catheters / Q000382:microbiology; D004353:Drug Evaluation, Preclinical; D004866:Equipment Contamination; D005898:Glass; D005944:Glucosamine / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D011137:Polystyrenes; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology
chemical_list: D016877:Oxidants; D011137:Polystyrenes; D005947:Glucose; D005944:Glucosamine