title: A versatile Agrobacterium-mediated transient gene expression system for herbaceous plants and trees.
abstract: Plant transient expression is a powerful method used widely for the functional characterization of genes and protein production. In comparison with stable transformation, it has the advantages of being simple, quick, economical, and effective. In the present study, we developed a novel transient gene expression system based on Agrobacterium-mediated transformation. This system is simple and convenient and allows for high transient expression levels. Hyperosmotic pretreatment of plants significantly improved the transient expression in this system. Furthermore, other factors, including acetosyringone concentration, cocultivation time, and Agrobacterium cell density, significantly influenced transient expression efficiency. The results showed that this method is suitable for use with herbaceous plants (such as tobacco and Arabidopsis) and trees (such as birch, poplar, tamarisk, cork, willow, and aralia), suggesting that it may be applied widely in plant transient expression studies.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.forename: Guifeng
authors.initials: G
authors.lastname: Liu
authors.forename: Xiangnan
authors.initials: X
authors.lastname: Meng
authors.forename: Yanbang
authors.initials: Y
authors.lastname: Li
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000098:Acetophenones; D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029662:Betula / Q000235:genetics / Q000378:metabolism; D018920:Coculture Techniques; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D013395:Sucrose; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014170:Transformation, Genetic
chemical_list: D000098:Acetophenones; D013395:Sucrose; C051667:acetosyringone; D005966:Glucuronidase