title: The expression of suppressor of cytokine signaling 1 and 3 in fat and placental tissue from women with gestational diabetes.
abstract: The suppressor of cytokine signaling (SOCS) proteins are feedback inhibitors of signaling pathways induced by cytokines, hormones and growth factors. In the present study we measured the expression of SOCS1, SOCS3, interleukin-6 (IL-6), IL-6 receptor, IL-8 and leptin mRNA in paired samples of subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT) and placental tissue obtained from 18 pregnant women with normal glucose tolerance (NGT) and 20 subjects with gestational diabetes mellitus (GDM), using quantitative RT-PCR. The patients with GDM had significantly higher IL-8 mRNA expression in VAT than the women with NGT (p = 0.007), whereas the expression of SOCS1, SOCS3 and other genes study did not differ significantly between the two groups. Stepwise regression analysis revealed that SOCS1 mRNA expression in VAT was significantly associated with prepregnancy BMI (β = -0.68, p = 0.03) and IL-8 mRNA expression (β = 0.66, p = 0.03), whereas SOCS3 mRNA expression in VAT was independently predicted by IL-6 mRNA expression (β = 0.94, p = 0.0002, R(2) = 0.88). In conclusion, our results did not show significant differences in SOCS1 and SOCS3 mRNA expression in adipose and placental tissue obtained from pregnant women with and without GDM.
authors.affiliation: Department of Gynecology, Medical University of Bialystok, M. Curie-Sklodowskiej 24A, Bialystok, Poland. mkuzmicki@op.pl
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kuzmicki
authors.forename: Beata
authors.initials: B
authors.lastname: Telejko
authors.forename: Natalia
authors.initials: N
authors.lastname: Wawrusiewicz-Kurylonek
authors.forename: Anna
authors.initials: A
authors.lastname: Citko
authors.forename: Danuta
authors.initials: D
authors.lastname: Lipinska
authors.forename: Justyna
authors.initials: J
authors.lastname: Pliszka
authors.forename: Juliusz
authors.initials: J
authors.lastname: Wilk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kalejta
authors.forename: Adam
authors.initials: A
authors.lastname: Lemancewicz
authors.forename: Marek
authors.initials: M
authors.lastname: Grabiec
authors.forename: Anna M
authors.initials: AM
authors.lastname: Pryszczepko-Wawreszuk
authors.forename: Justyna
authors.initials: J
authors.lastname: Skibicka
authors.forename: Adam
authors.initials: A
authors.lastname: Kretowski
authors.forename: Maria
authors.initials: M
authors.lastname: Gorska
authors.forename: Jacek
authors.initials: J
authors.lastname: Szamatowicz
mesh_terms: D000328:Adult; D016207:Cytokines / Q000378:metabolism; D016640:Diabetes, Gestational / Q000378:metabolism*; D005260:Female; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D050507:Subcutaneous Fat, Abdominal / Q000378:metabolism*; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000378:metabolism*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; C108944:SOCS1 protein, human; C106644:SOCS3 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins