title: Stress studies of tenofovir disoproxil fumarate by HPTLC in bulk drug and pharmaceutical formulation.
abstract: A stability-indicating high-performance thin-layer chromatographic (HPTLC) method for determination of tenofovir disoproxil fumarate in bulk drug and in tablet has been developed and validated. The mobile phase selected was chloroform:methanol (9.0:1.0, v/v) with ultraviolet (UV) detection at 260 nm. The retention factor was found to be 0.49 ± 0.03 with correlation coefficients of 0.9994 in the range 300-1500 ng/spot and with an accuracy of 99.25%. Method had the potential to determine tenofovir disoproxil fumarate from tablet without any interference, and it was a stability-indicating one.
authors.affiliation: Research and Development Centre in Pharmaceutical Sciences and Applied Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Havele
authors.forename: Sunil R
authors.initials: SR
authors.lastname: Dhaneshwar
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006868:Hydrolysis; D000432:Methanol; D063065:Organophosphonates / Q000032:analysis* / Q000737:chemistry; D010084:Oxidation-Reduction; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D010782:Photolysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets / Q000032:analysis; D000068698:Tenofovir; D014466:Ultraviolet Rays
chemical_list: D063065:Organophosphonates; D004364:Pharmaceutical Preparations; D013607:Tablets; D002725:Chloroform; D000068698:Tenofovir; D000225:Adenine; D000432:Methanol