title: Transcriptome profiling of Zymomonas mobilis under furfural stress.
abstract: Furfural from lignocellulosic hydrolysates is the prevalent inhibitor to microorganisms during cellulosic ethanol production, but the molecular mechanisms of tolerance to this inhibitor in Zymomonas mobilis are still unclear. In this study, genome-wide transcriptional responses to furfural were investigated in Z. mobilis using microarray analysis. We found that 433 genes were differentially expressed in response to furfural. Furfural up- or down-regulated genes related to cell wall/membrane biogenesis, metabolism, and transcription. However, furfural has a subtle negative effect on Entner-Doudoroff pathway mRNAs. Our results revealed that furfural had effects on multiple aspects of cellular metabolism at the transcriptional level and that membrane might play important roles in response to furfural. This research has provided insights into the molecular response to furfural in Z. mobilis, and it will be helpful to construct more furfural-resistant strains for cellulosic ethanol production.
authors.affiliation: Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Section 4-13, Renming Nanlu, Chengdu 610041, China. hemxion@hotmail.com
authors.forename: Ming-xiong
authors.initials: MX
authors.lastname: He
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Zong-xia
authors.initials: ZX
authors.lastname: Shui
authors.forename: Qi-chun
authors.initials: QC
authors.lastname: Hu
authors.forename: Wen-guo
authors.initials: WG
authors.lastname: Wang
authors.forename: Fu-rong
authors.initials: FR
authors.lastname: Tan
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Tang
authors.forename: Qi-li
authors.initials: QL
authors.lastname: Zhu
authors.forename: Ke
authors.initials: K
authors.lastname: Pan
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Su
mesh_terms: D001709:Biotechnology; D000431:Ethanol / Q000378:metabolism; D005662:Furaldehyde / Q000378:metabolism / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D016680:Genome, Bacterial; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D013312:Stress, Physiological; D017355:Zymomonas / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D005662:Furaldehyde