title: Lifetime-based photoacoustic oxygen sensing in vivo.
abstract: The determination of oxygen levels in blood and other tissues in vivo is critical for ensuring proper body functioning, for monitoring the status of many diseases, such as cancer, and for predicting the efficacy of therapy. Here we demonstrate, for the first time, a lifetime-based photoacoustic technique for the measurement of oxygen in vivo, using an oxygen sensitive dye, enabling real time quantification of blood oxygenation. The results from the main artery in the rat tail indicated that the lifetime of the dye, quantified by the photoacoustic technique, showed a linear relationship with the blood oxygenation levels in the targeted artery.
authors.affiliation: University of Michigan, Biophysics, Ann Arbor, MI 48109, USA.
authors.forename: Aniruddha
authors.initials: A
authors.lastname: Ray
authors.forename: Justin Rajesh
authors.initials: JR
authors.lastname: Rajian
authors.forename: Yong-Eun Koo
authors.initials: YE
authors.lastname: Lee
authors.forename: Xueding
authors.initials: X
authors.lastname: Wang
authors.forename: Raoul
authors.initials: R
authors.lastname: Kopelman
mesh_terms: D000818:Animals; D010092:Oximetry / Q000379:methods*; D010100:Oxygen / Q000097:blood*; D061088:Photoacoustic Techniques / Q000379:methods*; D011166:Porphyrins; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011166:Porphyrins; C488938:octaethylporphyrin; D010100:Oxygen