title: Platelet microparticles induce angiogenesis and neurogenesis after cerebral ischemia.
abstract: Activated platelets shed microparticles, which contain a variety of growth factors central to angiogenesis and neurogenesis. The aim of this study was to explore whether platelet derived microparticles (PMP) can boost endogenous neural stem cells dependent repair mechanisms following stroke in a rat model. To examine the effects of PMP therapy in-vivo, we delivered PMP or vehicle via a biodegradable polymer to the brain surface after permanent middle cerebral artery occlusion (PMCAO) in rats. Rats were tested with the neurological severity score and infarct volumes were measured at 90 days post-ischemia. Immunohistochemistry was used to determine the fate of newborn cells and to count blood vessels in the ischemic brain. The results show that PMP led to a dose dependent increase in cell proliferation, neurogenesis and angiogenesis at the infarct boundary zone and significantly improved behavioral deficits.
authors.affiliation: Department of Neurology, Peritz and Chantal Scheinberg Cerebrovascular Research Laboratory, Jerusalem, Israel.
authors.forename: Yael
authors.initials: Y
authors.lastname: Hayon
authors.forename: Olga
authors.initials: O
authors.lastname: Dashevsky
authors.forename: Ela
authors.initials: E
authors.lastname: Shai
authors.forename: Alex
authors.initials: A
authors.lastname: Brill
authors.forename: David
authors.initials: D
authors.lastname: Varon
authors.forename: Ronen R
authors.initials: RR
authors.lastname: Leker
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001792:Blood Platelets / Q000737:chemistry* / Q000166:cytology; D002545:Brain Ischemia / Q000209:etiology / Q000628:therapy*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D055252:Cell-Derived Microparticles / Q000502:physiology*; D004185:Disability Evaluation; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D020244:Infarction, Middle Cerebral Artery / Q000150:complications; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000187:drug effects / Q000502:physiology; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D020127:Recovery of Function; D013997:Time Factors
chemical_list: D009419:Nerve Tissue Proteins; D001973:Bromodeoxyuridine