title: Phage displayed peptides/antibodies recognizing growth factors and their tyrosine kinase receptors as tools for anti-cancer therapeutics.
abstract: The basic idea of displaying peptides on a phage, introduced by George P. Smith in 1985, was greatly developed and improved by McCafferty and colleagues at the MRC Laboratory of Molecular Biology and, later, by Barbas and colleagues at the Scripps Research Institute. Their approach was dedicated to building a system for the production of antibodies, similar to a naïve B cell repertoire, in order to by-pass the standard hybridoma technology that requires animal immunization. Both groups merged the phage display technology with an antibody library to obtain a huge number of phage variants, each of them carrying a specific antibody ready to bind its target molecule, allowing, later on, rare phage (one in a million) to be isolated by affinity chromatography. Here, we will briefly review the basis of the technology and the therapeutic application of phage-derived bioactive molecules when addressed against key players in tumor development and progression: growth factors and their tyrosine kinase receptors.
authors.affiliation: Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, viale Europa, 11, 25123 Brescia, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Ronca
authors.affiliation: Fibroblast Reprogramming Unit, Department of Biomedical Sciences and Biotechnology, viale Europa, 11, 25123 Brescia, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Benzoni
authors.affiliation: Fibroblast Reprogramming Unit, Department of Biomedical Sciences and Biotechnology, viale Europa, 11, 25123 Brescia, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: De Luca
authors.affiliation: Fibroblast Reprogramming Unit, Department of Biomedical Sciences and Biotechnology, viale Europa, 11, 25123 Brescia, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Crescini
authors.affiliation: Fibroblast Reprogramming Unit, Department of Biomedical Sciences and Biotechnology, viale Europa, 11, 25123 Brescia, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Dell'Era
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000276:immunology / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000276:immunology / Q000494:pharmacology; D036022:Capsid Proteins / Q000276:immunology; D061505:Cell Surface Display Techniques; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000276:immunology*; D009369:Neoplasms / Q000188:drug therapy*; D019151:Peptide Library; D020794:Receptor Protein-Tyrosine Kinases / Q000276:immunology*
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents; D036022:Capsid Proteins; D036341:Intercellular Signaling Peptides and Proteins; D019151:Peptide Library; D020794:Receptor Protein-Tyrosine Kinases