title: [Establishment and characterization of a human lung adenocarcinoma cell line, Ch-Huang-1].
abstract: OBJECTIVE
  Lung cancer is a major worldwide health problem. The aim of this study is to establish a novel Chinese human lung adenocarcinoma cell line and examine its biological characteristics.
  
  
  METHODS
  Lung adenocarcinoma specimens were freshly resected during surgery. The tissues were incubated in vitro and the cell line was named Ch-Huang-1. The biological characteristics of the cells were investigated by light microscopy, chromosome analysis, and transplantation experiment.
  
  
  RESULTS
  Light microscopy revealed that cells from the primary tumor, Ch-Huang-1 cell line, and transplanted tumor possessed the characteristics of a malignant glandular epithelial tumor. The cell growth curve, doubling time, and mitotic index were also observed in vitro. Nuclear chromosome analysis revealed that the tumor was a subtriploid with a mode of 35-44 per cell. Tumor nodes were observed under the skin of nude mice by heterogenic transplantation.
  
  
  CONCLUSIONS
  The characteristics of the established cell line suggest that it is a newly established human adenocarcinoma cell line.
authors.affiliation: Department of Cardiothoracic Surgery, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China.
authors.forename: Bailing
authors.initials: B
authors.lastname: Li
authors.forename: Keng
authors.initials: K
authors.lastname: Zhong
authors.forename: Lei
authors.initials: L
authors.lastname: Jin
authors.forename: Yang
authors.initials: Y
authors.lastname: Yuan
authors.forename: Dejun
authors.initials: D
authors.lastname: Gong
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Liu
authors.forename: Shengdong
authors.initials: S
authors.lastname: Huang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009374:Neoplasms, Experimental / Q000235:genetics / Q000473:pathology*; D013997:Time Factors; D014183:Transplantation, Heterologous; D047368:Tumor Burden; D014407:Tumor Cells, Cultured