title: Screening and analyzing potential hepatotoxic compounds in the ethanol extract of Asteris Radix by HPLC/DAD/ESI-MS(n) technique.
abstract: A refined toxic fraction, Fr-2, from the ethanol extract of Aster tataricus roots was obtained by a toxic tracing isolation. The hepatotoxic effects of Fr-2 were proved by the level elevation of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in mice serum and further confirmed by the histopathological examination on mice liver. A simple method was developed for qualitative analysis of constituents in Fr-2 fraction by high-performance liquid chromatography with diode array detection (HPLC-DAD) and electrospray ionization tandem mass spectrometry (ESI-MS(n)). As a result, 35 compounds were detected and identified in Fr-2. Among them, 28 compounds are pentapeptides, including 18 cyclic and 10 linear ones, and the sum of their peak area occupied about 87% (71.2% for cyclic and 15.8% for linear) of total area of chromatographic peak. Other 7 compounds are phenols, occupied about 5.9% of total area of chromatographic peak. Moreover, the structures of 10 cyclic and 2 linear peptides were reported for the first time. The results suggested that the peptides, especially the cyclic ones, are probably the principal toxic substances in Fr-2 also in A. tataricus roots.
authors.affiliation: State Key Laboratory of Natural Medicines, Research Department of Pharmacognosy, China Pharmaceutical University, Longmian Road 639, Nanjing 211198, PR China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Liu
authors.forename: Peipei
authors.initials: P
authors.lastname: Cao
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Zhang
authors.forename: Xianghong
authors.initials: X
authors.lastname: Xu
authors.forename: Mian
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D008099:Liver / Q000187:drug effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010936:Plant Extracts; D000431:Ethanol