title: Protective effects of topical application of a poorly soluble antioxidant astaxanthin liposomal formulation on ultraviolet-induced skin damage.
abstract: Astaxanthin (Asx) would be expected to prevent ultraviolet (UV)-induced skin damage, as it is regarded as a potent antioxidative carotenoid in biological membranes. However, it is difficult to administer Asx topically to skin because of its poor water solubility. In this study, we attempted to solve this problem by preparing liposomes containing Asx (Asx-lipo), which were dispersible in the water phase, and therefore, suitable for topical application to the skin. Asx-lipo was shown to have potent scavenging ability against chemiluminescence-dependent singlet oxygen production in the water phase. When Asx-lipo was applied to skin before UV exposure, UV-induced skin thickening was prevented. Interestingly, collagen reduction induced by UV exposure was also prevented by preadministration of Asx-lipo. In addition, topical administration of Asx-lipo containing cationic lipid inhibited melanin production in skin exposed to UV. Consequently, we succeeded in preventing UV-induced skin damage using a topical application of a liposomal formulation containing Asx.
authors.affiliation: Department of Biophysical Chemistry, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Hama
authors.forename: Kanako
authors.initials: K
authors.lastname: Takahashi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Inai
authors.forename: Kanako
authors.initials: K
authors.lastname: Shiota
authors.forename: Ryota
authors.initials: R
authors.lastname: Sakamoto
authors.forename: Asako
authors.initials: A
authors.lastname: Yamada
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kanamura
authors.forename: Eiji
authors.initials: E
authors.lastname: Yamashita
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kogure
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology*; D007478:Iontophoresis; D008081:Liposomes / Q000737:chemistry; D008297:Male; D008543:Melanins / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D026082:Singlet Oxygen / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D012880:Skin Pigmentation / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays; D024341:Xanthophylls / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D008081:Liposomes; D008543:Melanins; D024341:Xanthophylls; D026082:Singlet Oxygen; C005948:astaxanthine