title: Enantiomeric resolution of five chiral pesticides on a Chiralpak IB-H column by SFC.
abstract: The enantiomeric separations of five chiral pesticides, diclofopmethyl, 1; benalaxy, 2; acetofenate, 3; myclobutanil, 4; and difenoconazole, 5, were conducted on a Chiralpak IB-H column by a packed-column supercritical fluid chromatography (p-SFC). All compounds, except difenoconazole and myclobutanil, were well resolved within 10 min. As the mobile phase polarity decreased through changing the percentage and the type of alcohol modifiers in the supercritical carbon dioxide (CO(2)), the retention time, the separation factors, and the resolution increased. However, based on the retention time and the resolution, the optimized separations were obtained with the mobile phase containing 10% 2-propanol for diclofop-methyl 1; benalaxy, 2; myclobutanil, 4; difenoconazole, 5; and containing 3% 2-propanol for acetofenate, 3. The optimized separation temperature was at 35°C under the supercritical fluid condition. The π-π interactions and the hydrogen bonding interactions between Chiralpak IB-H CSP and the analytes might be the main chiral discriminations on enantioseparation of these five pesticides.
authors.affiliation: Research Centre of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Jin
authors.forename: Weiliang
authors.initials: W
authors.lastname: Gao
authors.forename: Huayun
authors.initials: H
authors.lastname: Yang
authors.forename: Chunmian
authors.initials: C
authors.lastname: Lin
authors.forename: Weiping
authors.initials: W
authors.lastname: Liu
mesh_terms: D000438:Alcohols / Q000737:chemistry; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D006860:Hydrogen Bonding; D010575:Pesticides / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D000438:Alcohols; D010575:Pesticides