title: Cryopreservation of shoot tips and meristems: an overview of contemporary methodologies.
abstract: Cryopreservation is the storage of viable bioresources at ultra-low temperatures in liquid nitrogen (LN). This chapter provides an overview of those protocols most commonly used to cryopreserve in vitro derived shoot tips and meristems; they are described generically, as sequential technical steps, including preparative and cryogenic treatments and the morphogenetic assessment of recovery. The importance of translating research-generated methods into formal Standard Operating Procedures (SOPs) is considered.
authors.affiliation: Conservation, Environmental Science and Biotechnology, Damar, Cupar Muir, Fife, Scotland, UK. e.benson-damar@tiscali.co.uk
authors.forename: Erica E
authors.initials: EE
authors.lastname: Benson
authors.forename: Keith
authors.initials: K
authors.lastname: Harding
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D018519:Meristem / Q000254:growth & development*; D018520:Plant Shoots / Q000254:growth & development*