title: Improvement of antioxidant activity of peptides with molecular weights ranging from 1 to 10 kDa by PEF technology.
abstract: Egg white protein powder was hydrolyzed with Alcalase to produce antioxidant peptides. Then, the peptides were fractionated with ultrafiltration membranes. The peptides (1-10 kDa) were further treated by pulsed electric field (PEF) to investigate its effect on the antioxidant activity of the peptides. Antioxidant activity was evaluated using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition assay. The results indicated that optimal electric field intensity and standing times of PEF can enhance the antioxidant activity of the peptides. Therefore, a Box-Behnken design (BBD) with three independent variables including concentration, electric field intensity and pulse frequency was used to establish the regression equation of second-order response surface. The optimal conditions were as follows: concentration 8 mg/ml, electric field intensity 10 kV/cm and pulse frequency 2400 Hz. Under these conditions, the peptides antioxidant activity was 62.64% ± 0.98%. The present study demonstrated that the antioxidant activity of peptides (1-10 kDa) could be improved using PEF.
authors.affiliation: Laboratory of Nutrition and Functional Food Jilin University, 5333 Xi'an Road, Changchun 130062, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Songyi
authors.initials: S
authors.lastname: Lin
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Gregory
authors.initials: G
authors.lastname: Jones
authors.forename: Hung
authors.initials: H
authors.lastname: Trang
authors.forename: Jingbo
authors.initials: J
authors.lastname: Liu
authors.forename: Haiqing
authors.initials: H
authors.lastname: Ye
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010455:Peptides