title: Chlorantraniliprole susceptibility in Leptinotarsa decemlineata in the north Xinjiang Uygur autonomous region in China.
abstract: The Colorado potato beetle (Leptinotarsa decemlineata (Say)) in the north Xinjiang Uygur autonomous region has evolved resistance to various types of insecticides. Chlorantraniliprole is a novel anthranilic diamide insecticide that binds and activates ryanodine receptors. It exhibited excellent efficacy against L. decemlineata in several field trails in Europe. In the present paper, the susceptibility of L. decemlineata fourth-instar larvae derived from six field populations and L. decemlineata adults derived from three field populations to chlorantraniliprole was determined by a topical application. The fourth-instar larvae were substantially more susceptible to chlorantraniliprole than adults, although the range of susceptibility was far greater among the fourth-instar larvae. Regarding stomach toxicities, adult beetles were less susceptible to chlorantraniliprole than larvae. Chlorantraniliprole was most toxic to second-instar larvae, followed by third- and fourth-instar larvae. These data suggested that the appropriate timing for chlorantraniliprole spraying is the early larval stage. Moreover, the synergistic activities of chlorantraniliprole in combination with triphenyl phosphate, diethyl maleate, or piperonyl butoxide against fourth-instar larvae from two field populations and adults from one field population were tested. Piperonyl butoxide had synergistic effects with chlorantraniliprole against fourth-instar larvae but not against adult beetles. Conversely, triphenyl phosphate and diethyl maleate exerted little synergistic effects. It appears that there is a potential risk of resistance against chlorantraniliprole resulting from cytochrome P450 monooxygenase activity.
authors.affiliation: Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Jiang
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Lu
authors.forename: Wen-Chao
authors.initials: WC
authors.lastname: Guo
authors.forename: Zhen-Han
authors.initials: ZH
authors.lastname: Xia
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Fu
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Li
mesh_terms: D000818:Animals; D002681:China; D001517:Coleoptera / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007300:Insect Control; D019476:Insect Proteins / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D008298:Maleates / Q000378:metabolism; D010755:Organophosphates / Q000378:metabolism; D010882:Piperonyl Butoxide / Q000378:metabolism; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D008298:Maleates; D010755:Organophosphates; D062367:ortho-Aminobenzoates; C517733:chlorantranilipole; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase; D004950:Esterases; C014476:diethyl maleate; D010882:Piperonyl Butoxide; C005445:triphenyl phosphate