title: Actinotetraoses A-H: tetrasaccharide derivatives from a grasshopper-associated Amycolatopsis sp. HCa1.
abstract: Chemical investigation of the insect-derived actinomycete Amycolatopsis sp. HCa1 with NMR-guided fractionation led to the isolation of eight new tetrasaccharide derivatives containing an unusual nonreducing glucotetraose skeleton, named actinotetraoses A-H (1-8), and one known compound, tigloside (9). The structures of these compounds were determined on the basis of analyses of their spectroscopic data. The in vitro immunosuppressive activity and cytotoxicity of these compounds were evaluated by T cell viability and MTT assays, respectively. Only actinotetraose E (5) displayed weak immunosuppressive activity.
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, People's Republic of China.
authors.forename: Zhi Kai
authors.initials: ZK
authors.lastname: Guo
authors.forename: Gao Fei
authors.initials: GF
authors.lastname: Zhang
authors.forename: Rui Hua
authors.initials: RH
authors.lastname: Jiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.forename: Ren Xiang
authors.initials: RX
authors.lastname: Tan
authors.forename: Hui Ming
authors.initials: HM
authors.lastname: Ge
mesh_terms: D039903:Actinobacteria / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005285:Fermentation; D006110:Grasshoppers / Q000382:microbiology*; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D025282:Mycelium / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D009904:Optical Rotation; D013601:T-Lymphocytes / Q000187:drug effects*
chemical_list: D007166:Immunosuppressive Agents; D009844:Oligosaccharides; C575883:actinotetraose E