title: Development of a deletion and genetic linkage map for the 5A and 5B chromosomes of wheat (Triticum aestivum).
abstract: The aims of the present study were to provide deletion maps for wheat ( Triticum aestivum  L.) chromosomes 5A and 5B and a detailed genetic map of chromosome 5A enriched with popular microsatellite markers, which could be compared with other existing maps and useful for mapping major genes and quantitative traits loci (QTL). Physical mapping of 165 gSSR and EST-SSR markers was conducted by amplifying each primer pair on Chinese Spring, aneuploid lines, and deletion lines for the homoeologous group 5 chromosomes. A recombinant inbred line (RIL) mapping population that is recombinant for only chromosome 5A was obtained by crossing the wheat cultivar Chinese Spring and the disomic substitution line Chinese Spring-5A dicoccoides and was used to develop a genetic linkage map of chromosome  5A. A total of 67 markers were found polymorphic between the parental lines and were mapped in the RIL population. Sixty-three loci and the Q gene were clustered in three linkage groups ordered at a minimum LOD score of 5, while four loci remained unlinked. The whole genetic 5A chromosome map covered 420.2 cM, distributed among three linkage groups of 189.3, 35.4, and 195.5 cM. The EST sequences located on chromosomes 5A and 5B were used for comparative analysis against  Brachypodium distachyon  (L.) P. Beauv. and rice ( Oryza sativa  L.) genomes to resolve orthologous relationships among the genomes of wheat and the two model species.
authors.affiliation: Department of Agro-Forestry and Environmental Biology and Chemistry, Section of Genetics and Plant Breeding, University of Bari Aldo Moro, Via Amendola 165/A, 70126 - Bari, Italy. agata.gadaleta@agr.uniba.it
authors.forename: A
authors.initials: A
authors.lastname: Gadaleta
authors.forename: A
authors.initials: A
authors.lastname: Giancaspro
authors.forename: S L
authors.initials: SL
authors.lastname: Giove
authors.forename: S
authors.initials: S
authors.lastname: Zacheo
authors.forename: O
authors.initials: O
authors.lastname: Incerti
authors.forename: R
authors.initials: R
authors.lastname: Simeone
authors.forename: P
authors.initials: P
authors.lastname: Colasuonno
authors.forename: D
authors.initials: D
authors.lastname: Nigro
authors.forename: G
authors.initials: G
authors.lastname: Valè
authors.forename: L
authors.initials: L
authors.lastname: Cattivelli
authors.forename: M
authors.initials: M
authors.lastname: Stanca
authors.forename: A
authors.initials: A
authors.lastname: Blanco
mesh_terms: D001483:Base Sequence; D058431:Brachypodium / Q000235:genetics; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D017931:DNA Primers; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D008040:Genetic Linkage; D018745:Genome, Plant; D018895:Microsatellite Repeats; D005810:Multigene Family; D012275:Oryza / Q000235:genetics; D040641:Quantitative Trait Loci; D017384:Sequence Deletion; D014908:Triticum / Q000235:genetics*
chemical_list: D017931:DNA Primers