title: Detailed structural analysis of lipids directly on tissue specimens using a MALDI-SpiralTOF-Reflectron TOF mass spectrometer.
abstract: Direct tissue analysis using a novel tandem time-of-flight (TOF-TOF) mass spectrometer is described. This system consists of a matrix-assisted laser desorption/ionization ion source, a spiral ion trajectory TOF mass spectrometer "SpiralTOF (STOF)", a collision cell, and an offset parabolic reflectron (RTOF). The features of this system are high precursor ion selectivity due to a 17-m flight path length in STOF and elimination of post-source decay (PSD) ions. The acceleration energy is 20 keV, so that high-energy collision-induced dissociation (HE-CID) is possible. Elimination of PSD ions allows observation of the product ions inherent to the HE-CID process. By using this tandem TOF instrument, the product ion spectrum of lipids provided detailed structural information of fatty acid residues.
authors.affiliation: Venture Business Laboratory, Osaka University, Suita, Osaka, Japan. sshimma@ncc.go.jp
authors.forename: Shuichi
authors.initials: S
authors.lastname: Shimma
authors.forename: Ayumi
authors.initials: A
authors.lastname: Kubo
authors.forename: Takaya
authors.initials: T
authors.lastname: Satoh
authors.forename: Michisato
authors.initials: M
authors.lastname: Toyoda
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D007477:Ions / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D007477:Ions; D008055:Lipids