title: Optimization of soy isoflavone extraction with different solvents using the simplex-centroid mixture design.
abstract: The objective of this study was to optimize the extraction of different isoflavone forms (glycosidic, malonyl-glycosidic, aglycone and total) from defatted cotyledon soy flour using the simplex-centroid experimental design with four solvents of varying polarity (water, acetone, ethanol and acetonitrile). The obtained extracts were then analysed by high-performance liquid chromatography. The profile of the different soy isoflavones forms varied with different extractions solvents. Varying the solvent or mixture used, the extraction of different isoflavones was optimized using the centroid-simplex mixture design. The special cubic model best fitted to the four solvents and its combination for soy isoflavones extraction. For glycosidic isoflavones extraction, the polar ternary mixture (water, acetone and acetonitrile) achieved the best extraction; malonyl-glycosidic forms were better extracted with mixtures of water, acetone and ethanol. Aglycone isoflavones, water and acetone mixture were best extracted and total isoflavones, the best solvents were ternary mixture of water, acetone and ethanol.
authors.affiliation: Department of Food Science and Technology, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
authors.forename: Luciane Yuri
authors.initials: LY
authors.lastname: Yoshiara
authors.forename: Tiago Bervelieri
authors.initials: TB
authors.lastname: Madeira
authors.forename: Fernanda
authors.initials: F
authors.lastname: Delaroza
authors.forename: Josemeyre Bonifácio
authors.initials: JB
authors.lastname: da Silva
authors.forename: Elza Iouko
authors.initials: EI
authors.lastname: Ida
mesh_terms: D002851:Chromatography, High Pressure Liquid; D018548:Cotyledon / Q000737:chemistry; D055664:Electrochemical Techniques; D006027:Glycosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D007529:Isoflavones / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D057230:Limit of Detection; D008956:Models, Chemical; D048789:Phytoestrogens / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D045730:Soy Foods / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry; D013223:Statistics as Topic
chemical_list: D006027:Glycosides; D007529:Isoflavones; D048789:Phytoestrogens; D010936:Plant Extracts; D012997:Solvents