title: Fluorescent staining of gels.
abstract: Certain transition metal complexes show intensive fluorescence when bound to proteins. They can be used to stain gels after electrophoresis with a sensitivity approaching that of silver staining, but in a much simpler and more reproducible procedure. Stains can be prepared easily and at a fraction of the cost of commercially available reagents.Hydrophobic dyes can be used to stain gels without fixing; they do not interfere with later blotting or electro-elution.
authors.affiliation: Ross University School of Medicine, Roseau, Commonwealth of Dominica, West Indies. engelbert_buxbaum@web.de
authors.forename: Engelbert
authors.initials: E
authors.lastname: Buxbaum
mesh_terms: D002021:Buffers; D003890:Desiccation / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010078:Oxazines / Q000737:chemistry*; D010618:Phenanthrolines / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D002021:Buffers; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D010078:Oxazines; D010618:Phenanthrolines; D011506:Proteins; C518776:ruthenium II tris (bathophenanthroline disulfonate); C044808:nile red