title: Temperature-modulated fluorescence tomography based on both concentration and lifetime contrast.
abstract: It is challenging to image fluorescence objects with high spatial resolution in a highly scattering medium. Recently reported temperature-sensitive indocyanine green-loaded pluronic nanocapsules can potentially alleviate this problem. Here we demonstrate a frequency-domain temperature-modulated fluorescence tomography system that could acquire images at high intensity-focused ultrasound resolution with use of these nanocapsules. The system is experimentally verified with a phantom study, where a 3-mm fluorescence object embedded 2 cm deep in a turbid medium is successfully recovered based on both intensity and lifetime contrast.
authors.affiliation: University of California, Department of Radiological Sciences, Tu and Yuen Center for Functional Onco-Imaging, Irvine, CA 92697, USA. yutingl@uci.edu
authors.forename: Yuting
authors.initials: Y
authors.lastname: Lin
authors.forename: Tiffany C
authors.initials: TC
authors.lastname: Kwong
authors.forename: Linden
authors.initials: L
authors.lastname: Bolisay
authors.forename: Gultekin
authors.initials: G
authors.lastname: Gulsen
mesh_terms: D003287:Contrast Media; D007089:Image Enhancement / Q000379:methods*; D007208:Indocyanine Green; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D053769:Nanocapsules; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods*
chemical_list: D003287:Contrast Media; D053769:Nanocapsules; D007208:Indocyanine Green