title: TEMED-enhanced photoluminescent imaging of human serum proteins by quantum dots after PAGE.
abstract: Polyacrylamide gel electrophoresis (PAGE) has been one of the most powerful and widely used separation techniques for complex biological samples, whose traditional detection methods include organic dye or silver staining. As a simple, convenient, and ultrasensitive detection of proteins for PAGE, a novel enhanced photoluminescent (PL) imaging method was developed. Thioglycolic acid (TGA)-capped CdTe quantum dots (QDs) and the enhanced reagent of tetramethylethylenediamine (TEMED) are introduced, achieving the direct detection of various proteins in native 1-DE, 2-DE, and SDS gels. Here, we describe the general protocol of TEMED-enhanced PL imaging by QDs, including materials, practical procedures, as well as some notes.
authors.affiliation: Beijing Normal University, Beijing, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Na
authors.forename: Jin
authors.initials: J
authors.lastname: Ouyang
mesh_terms: D001798:Blood Proteins / Q000737:chemistry* / Q000302:isolation & purification; D019187:Cadmium Compounds / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods / Q000592:standards; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000592:standards; D005029:Ethylenediamines / Q000737:chemistry*; D006801:Humans; D049449:Luminescence; D045663:Quantum Dots; D012015:Reference Standards; D012394:Rosaniline Dyes / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*; D013691:Tellurium / Q000737:chemistry; D013864:Thioglycolates / Q000737:chemistry
chemical_list: D001798:Blood Proteins; D019187:Cadmium Compounds; D004396:Coloring Agents; D005029:Ethylenediamines; D012394:Rosaniline Dyes; D013864:Thioglycolates; C005798:N,N,N',N'-tetramethylethylenediamine; C024757:Coomassie brilliant blue R; C017487:2-mercaptoacetate; D013691:Tellurium; C028337:cadmium telluride