title: [Cytogenetic differences between adults and children with acute lymphoblastic leukemia: eight-probe fluorescence in situ hybridization and karyotype analyses].
abstract: OBJECTIVE
  To investigate the cytogenetic differences between children and adults with acute lymphoblastic leukemia (ALL) using eight-probe fluorescence in situ hybridization and karyotype analysis.
  
  
  METHODS
  Eight-probe (MYC, P16, E2A, TEL/AML1, BCR/ABL , MLL , IGH, and hyperdiploidy) fluorescence in situ hybridization and karyotype analysis were performed for  86 adults and 39 children with acute lymphoblastic leukemia.
  
  
  RESULTS
  Eight-probe fluorescence in situ hybridization showed significant differences in the positivity rate of TEL/AML1, BCR/ABL, and hyperdiploidy between adult patients and children with ALL. By karyotype analysis, the positivity rate of t(9;22) and hyperdiploidy differed significantly between the children and adult patients (P<0.05).
  
  
  CONCLUSIONS
  Adults and children with ALL have different expression profiles of the fusion genes. Eight-probe fluorescence in situ hybridization is time-saving, accurate and efficient in detecting common genetic abnormalities in ALL patients, and can be well complementary to karyotype analysis in clinical diagnosis of ALL.
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, China. nmgzy4568@126.com
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zuo
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Du
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Xu
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.forename: Libin
authors.initials: L
authors.lastname: Liao
authors.forename: Lulu
authors.initials: L
authors.lastname: Xu
authors.forename: Jinfang
authors.initials: J
authors.lastname: Zhang
authors.forename: Bintao
authors.initials: B
authors.lastname: Huang
authors.forename: Xujing
authors.initials: X
authors.lastname: Luo
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Xiao
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003582:Cytogenetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007223:Infant; D059785:Karyotype; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000235:genetics*; D055815:Young Adult