title: [Preparation of a monoclonal antibody against fusion protein preM/EIII of WNV].
abstract: OBJECTIVE
  To prepare a monoclonal antibody (mAb) against the fusion protein preM/EIII of West Nile virus (WNV) for clinical detection of WNV.
  
  
  METHODS
  Sp2/0 cells were fused with the spleen cells of BALB/c mice immunized with the recombinant fusion protein preM/EIII expressed in E. coil to obtain the hybridoma cell line that secreted preM/EIII mAb. The hybridoma cells were injected into the peritoneal cavity of BALB/c mice and the ascites was collected and purified. The specificity and titer of the obtained mAb were determined using ELISA and Western blotting.
  
  
  RESULTS
  One hybridoma cell line secreting preM/EIII mAb, named ME1, was obtained. The titer of the purified mAb was 10(-6). Identified as a mAb of the Ig subclass IgG1, ME1 was capable of specific reactions with preM/EIII protein and WNV without cross-reactions with other viruses such as JEV, SLEV, YFV and DENV. The accuracy of clinical testing of MNV with ME1 was 97.78%.
  
  
  CONCLUSIONS
  The mAb against preM/EIII obtained have a high specificity and accuracy in clinical testing of MNV and can be used in clinical diagnosis of MNV infection.
authors.affiliation: Department of Laboratory Medicine, Guangzhou General Hospital of Guangzhou Military Comman, Guangzhou, China. mature303@126.com
authors.forename: Linhai
authors.initials: L
authors.lastname: Li
authors.forename: Lidan
authors.initials: L
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Liao
authors.forename: Jianyun
authors.initials: J
authors.lastname: Chen
authors.forename: Yuling
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D003429:Cross Reactions; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D014760:Viral Fusion Proteins / Q000276:immunology*; D014902:West Nile virus / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D014760:Viral Fusion Proteins