title: Development and characterization of microsatellite  markers for the endangered Chinese yew Taxus chinensis var. mairei (Taxaceae).
abstract: We isolated and characterized 11 microsatellite loci  for the Chinese yew, Taxus chinensis var. mairei, an endangered  tree species in China, by constructing a (CA)(12)-enriched library. The number of alleles  per locus ranged from 5 to 10. The observed heterozygosities ranged from 0.2500  to 0.8333 and the expected heterozygosities ranged from 0.5196 to 0.8680. No  significant linkage disequilibrium was detected at these loci. However, four  loci significantly deviated from Hardy-Weinberg equilibrium. The null alleles  were found to be present at locus Tach9 and locus Tach11 by the Micro-checker  test (P < 0.001). These polymorphic loci could be employed in research of  gene flow and spatial genetic patterns of T. chinensis var. mairei.
authors.affiliation: College of Forest Resources and Environment, Nanjing Forestry University, Nanjing, China.
authors.forename: H
authors.initials: H
authors.lastname: Xue
authors.forename: C H
authors.initials: CH
authors.lastname: Lu
authors.forename: X B
authors.initials: XB
authors.lastname: Wu
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D056727:Endangered Species; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D032490:Taxaceae / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers