title: Conformational preferences of 4-chloroproline residues.
abstract: Conformational preferences of the (2S,4R)-4-chloroproline (Clp) and (2S,4S)-4-chloroproline (clp) residues are explored at the M06-2X/cc-pVTZ//M06-2X/6-31+G(d) level of theory in the gas phase and in water, where solvation free energies were calculated using the implicit solvation model, and by an X-ray diffraction study in the solid state. In the gas phase, the down-puckered γ-turn structure with the trans prolyl peptide bond is most preferred for both Ac-Clp-NHMe and Ac-clp-NHMe, in which the C(7) hydrogen bond between two terminal groups seems to play a role, as found for Ac-Pro-NHMe. In water, the Clp residue has a strong preference for the up-puckered PP(II) structure, whereas the up-puckered PP(II) structure prevails a little over the down-puckered PP(II) structure for the clp residue, similar to the Pro residue. Hence, our calculated results on the puckering preference of the Clp and clp residues in water are in accord with the observed results deduced from the relative stabilities of the triple helices of the collagen model peptides. The X-ray structure of Ac-clp-NHMe was found to be the most preferred in water but that of Ac-Clp-NHMe was located as a local minimum with ΔG = 2.0 kcal/mol. In particular, the X-ray structure of Ac-Clp-NHMe was quite different from that of Ac-Clp-OMe but similar to that of Ac-Pro-NHMe. The lowest rotational barriers to the prolyl cis-trans isomerization for Ac-Clp-NHMe become nearly the same as those for Ac-Pro-NHMe in water, whereas the barriers are lower by ∼2 kcal/mol for Ac-clp-NHMe. It was found that the cis-trans isomerization may proceed through the clockwise or anticlockwise rotations for Ac-Clp-NHMe and the anticlockwise rotation for Ac-clp-NHMe and Ac-Pro-NHMe in water.
authors.affiliation: Department of Nursing, Cheju-halla College, Cheju, Republic of Korea.
authors.forename: Hae Sook
authors.initials: HS
authors.lastname: Park
authors.forename: Byung Jin
authors.initials: BJ
authors.lastname: Byun
authors.forename: Daisuke
authors.initials: D
authors.lastname: Motooka
authors.forename: Kazuki
authors.initials: K
authors.lastname: Kawahara
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Doi
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakazawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Young Kee
authors.initials: YK
authors.lastname: Kang
mesh_terms: D004151:Dipeptides / Q000737:chemistry; D005740:Gases / Q000737:chemistry; D006860:Hydrogen Bonding; D007536:Isomerism; D008968:Molecular Conformation; D011392:Proline / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D013816:Thermodynamics; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: C530187:4-chloroproline; D004151:Dipeptides; D005740:Gases; D012996:Solutions; D014867:Water; D011392:Proline