title: Shared features of S100B immunohistochemistry and cytochrome oxidase histochemistry in the ventroposterior thalamus and lateral habenula in neonatal rats.
abstract: The ventroposterior thalamus and the habenular nuclei of the epithalamus are relevant to the monoaminergic system functionally and anatomically. The glia-derived S100B protein plays a critical role in the development of the nervous system including the monoaminergic systems. In this study, we performed an immunohistochemical study of glia-related proteins including S100B, serotonin transporter, and microtubule-associated protein 2, as well as cytochrome oxidase histochemistry in neonatal rats. Results showed the same findings for S100B immunohistochemistry between the ventroposterior thalamus and the lateral habenula at postnatal day 7: intense staining in cell bodies of astrocytes, diffusely spread immunoproduct in the intercellular space, and S100B-free areas as well as a strong reaction to cytochrome oxidase histochemistry. Further common features were the scarcity of glial fibrillary acidic protein-positive astrocytes and the few apoptotic cells observed. The results of the cytochrome oxidase reaction suggested that S100B is released actively into intercellular areas in restricted brain regions showing high neuronal activity at postnatal day 7. Pathology of the ventroposterior thalamus and the habenula is suggested in mental disorders, and S100B might be a key factor for investigations in these areas.
authors.affiliation: Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-Ku, Tokyo 142-8555, Japan. kmuneoka@med.showa-u.ac.jp
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Muneoka
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Funahashi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Ogawa
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Whitaker-Azmitia
authors.forename: Seiji
authors.initials: S
authors.lastname: Shioda
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D019262:Habenula / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000378:metabolism; D020651:Ventral Thalamic Nuclei / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579158:S100b protein, rat; D050486:Serotonin Plasma Membrane Transport Proteins; D003576:Electron Transport Complex IV