title: Inducible and constitutive activation of two polymorphic promoter alleles of the Candida albicans multidrug efflux pump MDR1.
abstract: Overexpression of the multidrug efflux pump MDR1 confers resistance to the antifungal drug fluconazole on Candida albicans. It has been reported that two types of MDR1 promoters exist in C. albicans and that homozygosity for the allele with higher activity may promote fluconazole resistance. We found that the two MDR1 promoter alleles in strain SC5314 were equally well activated by inducing chemicals or hyperactive forms of the transcription factors Mrr1 and Cap1, which control MDR1 expression. In addition, no loss of heterozygosity at the MDR1 locus was observed in MDR1-overexpressing clinical C. albicans strains that developed fluconazole resistance during therapy.
authors.affiliation: Institut für Molekulare Infektionsbiologie, Universität Würzburg, Würzburg, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Sasse
authors.forename: Rebecca
authors.initials: R
authors.lastname: Schillig
authors.forename: Alexandra
authors.initials: A
authors.lastname: Reimund
authors.forename: Julia
authors.initials: J
authors.lastname: Merk
authors.forename: Joachim
authors.initials: J
authors.lastname: Morschhäuser
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis / Q000235:genetics*; D000483:Alleles; D000935:Antifungal Agents / Q000494:pharmacology*; D050976:Basic-Leucine Zipper Transcription Factors; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins; D026141:Drug Resistance, Multiple, Fungal / Q000235:genetics; D015725:Fluconazole / Q000494:pharmacology*; D005656:Fungal Proteins; D019656:Loss of Heterozygosity / Q000235:genetics; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000935:Antifungal Agents; D050976:Basic-Leucine Zipper Transcription Factors; C426523:CAP1 protein, Candida albicans; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D014157:Transcription Factors; D015725:Fluconazole