title: Cellular infiltration on nanofibrous scaffolds using a modified electrospinning technique.
abstract: Electrospinning is currently used to fabricate nanofibrous scaffolds for tissue engineering applications. The major problem of these scaffolds is their intrinsically two-dimensional nature which inhibits cellular migration and in-growth. In this study, we have introduced a modified setup of electrospinning to produce three-dimensional nanofibrous scaffolds which allows improved infiltration of cells. An array of focused halogen light bulbs was used to localize the heat in the path of electrospun jet near the collector. The fabricated mats were then seeded with cells in order to evaluate migration and infiltration. After 14 days of culture, a homogenous distribution of cells was observed throughout the scaffolds and showed the three-dimensional architecture of nanofibrous mats. By this novel and simple setup, the prepared electrospun mats will allow the seeded cells to obtain a three-dimensional arrangement which is ideal for tissue engineering applications.
authors.affiliation: Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Iman
authors.initials: I
authors.lastname: Shabani
authors.forename: Vahid
authors.initials: V
authors.lastname: Haddadi-Asl
authors.forename: Ehsan
authors.initials: E
authors.lastname: Seyedjafari
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D018929:Cell Culture Techniques; D002465:Cell Movement; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D016062:Porosity; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*