title: Identification of neural stem cells in the Drosophila larval brain.
abstract: The balance between self-renewal and differentiation must be tightly regulated in somatic stem cells to ensure proper tissue generation and to prevent tumorlike overgrowth. A Drosophila larval brain lobe consists of the central brain and the optic lobe and possesses three well-defined neural stem cell lineages that generate differentiated cells in a highly reproducible pattern. Unambiguous identification of various cell types in these stem cell lineages is pivotal for studying the regulation of neural stem cells and progenitor cells at a single-cell resolution. This chapter will describe the methodology for collection and processing of larval brains for examination by fluorescence confocal microscopy.
authors.affiliation: Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Mo
authors.initials: M
authors.lastname: Weng
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Komori
authors.forename: Cheng-Yu
authors.initials: CY
authors.lastname: Lee
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D004331:Drosophila melanogaster / Q000166:cytology*; D007814:Larva / Q000166:cytology; D058953:Neural Stem Cells / Q000166:cytology*