title: Cell-penetrating peptide mimicking polymer-based combined delivery of paclitaxel and siRNA for enhanced tumor growth suppression.
abstract: Cancer chemotherapy is often limited, since more than one molecule is usually involved with the cancer pathogenesis. A combination of therapeutic drugs would be a promising approach to overcome the complexity of tumors. In this study, a conjugate (DA3) of deoxycholic acid and low molecular weight polyethylenimine (PEI 1.8 kDa), which has a property that mimics that of cell penetrating peptides (CPPs), was used for simultaneous delivery of an anticancer drug and siRNA. When complexed with siRNA, DA3 showed significantly higher target gene silencing efficiency than PEI 25 kDa. The gene silencing efficiency of DA3 was maintained even in the presence of endocytosis inhibitors, suggesting that the polymeric carrier can mediate an endocytosis-independent macromolecular transduction similar to CPPs. The capability of forming a micelle-like core-shell structure enables the conjugates to encapsulate and dissolve paclitaxel (PTX), a water-insoluble drug. The drug-loaded cationic micelles can then interact with siRNA to form stable complexes (PTX/DA3/siRNA). The PTX/DA3/siRNA showed significantly enhanced inhibition of cancer cell growth. When administered into tumor-bearing animals, the PTX/DA3/siRNA demonstrated significant suppression of tumor growth, providing potential usefulness in clinical settings.
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Yeon Lim
authors.initials: YL
authors.lastname: Jang
authors.forename: Ui Jeong
authors.initials: UJ
authors.lastname: Yun
authors.forename: Min Sang
authors.initials: MS
authors.lastname: Lee
authors.forename: Myung Goo
authors.initials: MG
authors.lastname: Kim
authors.forename: Sohee
authors.initials: S
authors.lastname: Son
authors.forename: Kyuri
authors.initials: K
authors.lastname: Lee
authors.forename: Su Young
authors.initials: SY
authors.lastname: Chae
authors.forename: Dong Woo
authors.initials: DW
authors.lastname: Lim
authors.forename: Hong Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Kim
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Jeong
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D057846:Cell-Penetrating Peptides / Q000737:chemistry; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D003840:Deoxycholic Acid / Q000737:chemistry; D016503:Drug Delivery Systems; D004705:Endocytosis; D020868:Gene Silencing; D045325:HCT116 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008823:Micelles; D008970:Molecular Weight; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D011094:Polyethyleneimine / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D012995:Solubility
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D057846:Cell-Penetrating Peptides; D008823:Micelles; D034741:RNA, Small Interfering; D003840:Deoxycholic Acid; D011094:Polyethyleneimine; D017239:Paclitaxel