title: Synaptic distributions of GluA2 and PKMζ in the monkey dentate gyrus and their relationships with aging and memory.
abstract: Rhesus monkeys provide a valuable model for studying the neurobiological basis of cognitive aging, because they are vulnerable to age-related memory decline in a manner similar to humans. In this study, young and aged monkeys were first tested on a well characterized recognition memory test (delayed nonmatching-to-sample; DNMS). Then, electron microscopic immunocytochemistry was performed to determine the subcellular localization of two proteins in the hippocampal dentate gyrus (DG): the GluA2 subunit of the glutamate AMPA receptor and the atypical protein kinase C ζ isoform (PKMζ). PKMζ promotes memory storage by regulating GluA2-containing AMPA receptor trafficking. Thus, we examined whether the distribution of GluA2 and PKMζ is altered with aging in DG axospinous synapses and whether it is coupled with memory deficits. Monkeys with faster DNMS task acquisition and more accurate recognition memory exhibited higher proportions of dendritic spines coexpressing GluA2 and PKMζ. These double-labeled spines had larger synapses, as measured by postsynaptic density area, than single-labeled and unlabeled spines. Within this population of double-labeled spines, aged monkeys compared with young expressed a lower density of synaptic GluA2 immunogold labeling, which correlated with lower recognition accuracy. Additionally, higher density of synaptic PKMζ labeling in double-labeled spines correlated with both faster task acquisition and better retention. Together, these findings suggest that age-related impairment in maintenance of GluA2 at the synapse in the primate hippocampus is coupled with memory deficits.
authors.affiliation: Fishberg Department of Neuroscience and Kastor Neurobiology of Aging Laboratories, Mount Sinai School of Medicine, New York, New York 10029, USA.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hara
authors.forename: Michael
authors.initials: M
authors.lastname: Punsoni
authors.forename: Frank
authors.initials: F
authors.lastname: Yuk
authors.forename: C Sehwan
authors.initials: CS
authors.lastname: Park
authors.forename: William G M
authors.initials: WG
authors.lastname: Janssen
authors.forename: Peter R
authors.initials: PR
authors.lastname: Rapp
authors.forename: John H
authors.initials: JH
authors.lastname: Morrison
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D049229:Dendritic Spines / Q000378:metabolism / Q000648:ultrastructure; D018891:Dentate Gyrus / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D008253:Macaca mulatta; D008568:Memory / Q000502:physiology*; D008569:Memory Disorders / Q000378:metabolism / Q000503:physiopathology; D009474:Neurons / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D011493:Protein Kinase C / Q000378:metabolism* / Q000502:physiology; D021381:Protein Transport / Q000502:physiology; D011597:Psychomotor Performance / Q000502:physiology; D018091:Receptors, AMPA / Q000378:metabolism* / Q000502:physiology; D021641:Recognition, Psychology / Q000502:physiology; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure; D000099221:Protein Kinase C zeta
chemical_list: D011493:Protein Kinase C; D018091:Receptors, AMPA; D000099221:Protein Kinase C zeta; C104722:glutamate receptor ionotropic, AMPA 2