title: Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis.
abstract: We present a design for a sheath-flow cuvette that uses a relatively inexpensive quartz cuvette. The cuvette has a high optical quality square flow chamber that is fused to quartz tubes at each end. PEEK/TEFZEL fittings hold and seal the quartz flow chamber without putting strain on the cuvette. The performance of the cuvette is evaluated as a laser-induced fluorescence detector for capillary electrophoresis. The cuvette produces mass detection limits of 50 yoctomoles (30 copies) for 5-carboxyl tetramethylrhodamine (5 TAMRA SE) with a separation efficiency of 400,000 theoretical plates.
authors.affiliation: Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA.
authors.forename: Oluwatosin O
authors.initials: OO
authors.lastname: Dada
authors.forename: Bonnie J
authors.initials: BJ
authors.lastname: Huge
authors.forename: Norman J
authors.initials: NJ
authors.lastname: Dovichi
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005434:Flow Cytometry; D057230:Limit of Detection; D013050:Spectrometry, Fluorescence / Q000379:methods*