title: A unique FACS method to isolate stem cells in planarian.
abstract: Fluorescence-activated cell sorting (FACS) is a useful method for stem cell biology, which enables us to isolate the living stem cells of interest from mixture of a variety of cells. In general, the target cells for FACS need to be labeled for various cell surface markers. However, in non-model organisms, we usually do not have specific labels for such cell surface markers. Here, we describe a method for isolating stem cells from non-model organisms, such as planarians, based on physiological and morphological properties of the stem cells. This method may also be applicable to other non-model animals.
authors.affiliation: Genome Resource and Analysis Unit, RIKEN Center for Developmental Biology, Chuo-ku, Kobe, Hyogo, Japan.
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Hayashi
authors.forename: Kiyokazu
authors.initials: K
authors.lastname: Agata
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D010932:Planarians / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*