title: AhNRAMP1 iron transporter is involved in iron acquisition in peanut.
abstract: Peanut/maize intercropping is a sustainable and effective agroecosystem to alleviate iron-deficiency chlorosis. Using suppression subtractive hybridization from the roots of intercropped and monocropped peanut which show different iron nutrition levels, a peanut gene, AhNRAMP1, which belongs to divalent metal transporters of the natural resistance-associated macrophage protein (NRAMP) gene family was isolated. Yeast complementation assays suggested that AhNRAMP1 encodes a functional iron transporter. Moreover, the mRNA level of AhNRAMP1 was obviously induced by iron deficiency in both roots and leaves. Transient expression, laser microdissection, and in situ hybridization analyses revealed that AhNRAMP1 was mainly localized on the plasma membrane of the epidermis of peanut roots. Induced expression of AhNRAMP1 in tobacco conferred enhanced tolerance to iron deprivation. These results suggest that the AhNRAMP1 is possibly involved in iron acquisition in peanut plants.
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China.
authors.forename: Hongchun
authors.initials: H
authors.lastname: Xiong
authors.forename: Takanori
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kakei
authors.forename: Takeshi
authors.initials: T
authors.lastname: Senoura
authors.forename: Mikio
authors.initials: M
authors.lastname: Nakazono
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Takahashi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Hongyun
authors.initials: H
authors.lastname: Shen
authors.forename: Penggen
authors.initials: P
authors.lastname: Duan
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Guo
authors.forename: Naoko K
authors.initials: NK
authors.lastname: Nishizawa
authors.forename: Yuanmei
authors.initials: Y
authors.lastname: Zuo
mesh_terms: D010367:Arachis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D005816:Genetic Complementation Test; D007501:Iron / Q000378:metabolism*; D000090463:Iron Deficiencies; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D019441:Plant Epidermis / Q000196:embryology / Q000235:genetics / Q000502:physiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D036226:Seedlings / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D003313:Zea mays / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D027682:Cation Transport Proteins; D007501:Iron; D008565:Membrane Proteins; D010940:Plant Proteins; D000099173:Natural Resistance-associated Macrophage Protein 1