title: Hematological parameters' changes in mice subchronically exposed to static magnetic fields of different orientations.
abstract: Static magnetic fields (SMFs) are time independent fields whose intensity can be spatially dependent. This study investigates influence of subchronic continuous exposure to upward and downward directed SMF on hematological parameters and spleen cellularity in mice. The experiment is performed on the Northern hemisphere; consequently, the vertical component of geomagnetic field is directed downward. Male, Swiss-Webster, 6 weeks old mice were exposed to the vertically declining SMF. Mice were divided in three groups and continuously exposed or not exposed for 28 days to the SMF characterized by the averaged field of 16 mT and averaged field gradient of 10 mT/cm. Differently oriented SMF did not alter hemoglobin and hematocrit content among the groups. However, the groups exposed to the upward and downward fields had statistically significant higher levels of serum transferrin compared to the control. Moreover, spleen cellularity in animals in the downward group was significantly higher compared to the upward and control group. In addition, spleen lymphocytes in both of the exposed groups were significantly higher than in the control group. In contrast, spleen granulocytes in the exposed groups were significantly lower than in the control group. Significant decrease was also observed in brain and liver iron content with concomitant increase of iron in serum and spleen in exposed animals. Subchronic continuous exposure to 16 mT SMF caused lymphocyte and granulocyte redistribution between spleen and blood. This distribution is typical for stress induced hematological changes. These results suggest that observed changes were not due to an unspecific stress response, but that they were rather caused by specific adaptation to subchronic SMF exposure.
authors.affiliation: Department of Pathological Physiology, School of Medicine, University of Belgrade, Dr Subotića 9, 11000 Belgrade, Serbia.
authors.forename: Drago M
authors.initials: DM
authors.lastname: Djordjevich
authors.forename: Silvio R
authors.initials: SR
authors.lastname: De Luka
authors.forename: Ivan D
authors.initials: ID
authors.lastname: Milovanovich
authors.forename: Saša
authors.initials: S
authors.lastname: Janković
authors.forename: Srdjan
authors.initials: S
authors.lastname: Stefanović
authors.forename: Slavica
authors.initials: S
authors.lastname: Vesković-Moračanin
authors.forename: Saša
authors.initials: S
authors.lastname: Cirković
authors.forename: Andjelija Ž
authors.initials: AŽ
authors.lastname: Ilić
authors.forename: Jasna L
authors.initials: JL
authors.lastname: Ristić-Djurović
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Trbovich
mesh_terms: D000818:Animals; D008099:Liver / Q000502:physiology; D008214:Lymphocytes / Q000473:pathology; D060526:Magnetic Fields / Q000009:adverse effects*; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000473:pathology