title: Age-related expression, enzymatic solubility and modification with advanced glycation end-products of fibrillar collagens in mouse lung.
abstract: Changes in the expression of fibrillar collagens and post-translational modifications with advanced glycation end-products (AGEs) are often associated with tissue aging. Less is known about age-related changes in mouse lung tissue. Therefore, we studied the expression level and AGE load of fibrillar collagens in lungs from young (≤6 months), adult (15 months) and old (≥25 months) mice. The mRNA expression level was reduced in adult and old mice compared with the young. Old mice also showed a reduced protein level, whereas the adults even had more collagen protein. Fractionating of the fibrillar collagens into enzyme-soluble and insoluble collagens revealed a reduced solubility of collagens in old age. The enzymatic solubility of fibrillar collagens correlated inversely with the AGE load in the insoluble collagen as detected by the AGE-related fluorescence. While the intensity of the AGE-related fluorescence was increased in fibrillar collagens in response to age, the fluorescing AGE variant argpyrimidine was less affected. In summary, aging causes a reduced expression, lower enzymatic solubility and increased AGE load of fibrillar collagens in mouse lung tissue, but not all changes occur gradually with age.
authors.affiliation: Department of Cardio-thoracic Surgery, University Hospital Halle (Saale), Martin Luther University Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany.
authors.forename: Paulina
authors.initials: P
authors.lastname: Rolewska
authors.forename: Samiya
authors.initials: S
authors.lastname: Al-Robaiy
authors.forename: Alexander
authors.initials: A
authors.lastname: Navarrete Santos
authors.forename: Andreas
authors.initials: A
authors.lastname: Simm
authors.forename: Rolf-Edgar
authors.initials: RE
authors.lastname: Silber
authors.forename: Babett
authors.initials: B
authors.lastname: Bartling
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D024022:Fibrillar Collagens / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D017127:Glycation End Products, Advanced / Q000378:metabolism / Q000502:physiology*; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics; D012995:Solubility
chemical_list: D024022:Fibrillar Collagens; D017127:Glycation End Products, Advanced; D012333:RNA, Messenger