title: Preliminary study of thyroid and colon cancers-associated antigens and their cognate autoantibodies as potential cancer biomarkers.
abstract: BACKGROUND
  Autoantibodies, which are produced against tumor-associated antigens, are potential tumor markers and attract a growing interest for cancer detection, differential diagnostics and prognosis.
  
  
  OBJECTIVE
  To evaluate the diagnostic significance of 40 antigens identified by immunoscreening of cDNA libraries from thyroid and colon cancers by allogenic screening with different tumor types patients' sera.
  
  
  METHODS
  Plaque-spot serological assay.
  
  
  RESULTS
  Increased frequency of antibody response in sera of cancer patients compared with that of healthy donors was shown toward 14 antigens, 8 of which (CG016, BTN3A3, FKBP4, XRCC4, TSGA2, ACTR1A, FXYD3 and CTSH) have revealed exclusively cancer-related serological profile.
  
  
  CONCLUSIONS
  Allogenic screening of 40 SEREX-antigens with sera from cancer patients and healthy donors allowed us to reveal 14 antigens with potential diagnostic significance. These antigens and their cognate autoantibodies could be considered as valuable targets for further analysis as potential cancer biomarkers.
authors.affiliation: Department of Cell Signaling, Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv, Ukraine. r.g.kiyamova@imbg.org.ua
authors.forename: Ramziya
authors.initials: R
authors.lastname: Kiyamova
authors.forename: Oleg
authors.initials: O
authors.lastname: Garifulin
authors.forename: Vitalina
authors.initials: V
authors.lastname: Gryshkova
authors.forename: Olga
authors.initials: O
authors.lastname: Kostianets
authors.forename: Maksym
authors.initials: M
authors.lastname: Shyian
authors.forename: Ivan
authors.initials: I
authors.lastname: Gout
authors.forename: Valeriy
authors.initials: V
authors.lastname: Filonenko
mesh_terms: D000328:Adult; D000368:Aged; D000912:Antibodies, Neoplasm / Q000097:blood*; D000951:Antigens, Neoplasm / Q000097:blood* / Q000235:genetics / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics / Q000276:immunology; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D055088:Early Detection of Cancer; D015723:Gene Library; D006801:Humans; D008875:Middle Aged; D013964:Thyroid Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000276:immunology
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor