title: Serotypes and genetic profiles of Bordetella pertussis strains isolated in the city of São Paulo, 2006-2008.
abstract: OBJECTIVE
  Knowledge of Bordetella pertussis circulating in Latin America is limited. Therefore, the goal of this study was to use pulsed-field gel electrophoresis and serotyping to characterize B. pertussis strains isolated in the city of São Paulo, Brazil.
  
  
  METHODS
  This study, conducted between 2006 and 2008, analyzed 652 nasopharyngeal swabs from suspected pertussis cases and contacts, collected from 37 sentinel hospitals in São Paulo. Randomized samples of 91 (70%) strains of B. pertussis were subtyped by pulsed-field gel electrophoresis and serotyping.
  
  
  RESULTS
  Ninety-seven percent of strains from São Paulo were serotyped as Fim3. Fourteen pulsed-field gel electrophoresis profiles were identified; the most prevalent (57%) is also the most prevalent in the USA.
  
  
  CONCLUSIONS
  These data, in conjunction with surveillance activities, may impact strategies regarding prevention and control of pertussis in the region, providing useful information for introduction of new vaccination strategies and reduction of risk of transmission to infants less than 6 months of age.
authors.affiliation: Center of Bacteriology, National Reference Laboratory for Pertussis, Instituto Adolfo Lutz, São Paulo, SP, Brazil. dedeleite@gmail.com
authors.forename: Daniela
authors.initials: D
authors.lastname: Leite
authors.forename: Pamela K
authors.initials: PK
authors.lastname: Cassiday
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Tatti
authors.forename: Tânia Mara Ibelli
authors.initials: TM
authors.lastname: Vaz
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: Tondella
mesh_terms: D000293:Adolescent; D000367:Age Factors; D001886:Bordetella pertussis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010567:Pertussis Vaccine / Q000627:therapeutic use; D012703:Serotyping; D014611:Vaccination; D014917:Whooping Cough / Q000382:microbiology* / Q000517:prevention & control; D055815:Young Adult
chemical_list: D010567:Pertussis Vaccine