title: Phylogenetic reconstruction at the species and intraspecies levels in the genus Pisum (L.) (peas) using a histone H1 gene.
abstract: A phylogenetic analysis of the genus Pisum (peas), embracing diverse wild and cultivated forms, which evoke problems with species delimitation, was carried out based on a gene coding for histone H1, a protein that has a long and variable functional C-terminal domain. Phylogenetic trees were reconstructed on the basis of the coding sequence of the gene His5 of H1 subtype 5 in 65 pea accessions. Early separation of a clear-cut wild species Pisum fulvum is well supported, while cultivated species Pisum abyssinicum appears as a small branch within Pisum sativum. Another robust branch within P. sativum includes some wild and almost all cultivated representatives of P. sativum. Other wild representatives form diverse but rather subtle branches. In a subset of accessions, PsbA-trnH chloroplast intergenic spacer was also analysed and found less informative than His5. A number of accessions of cultivated peas from remote regions have a His5 allele of identical sequence, encoding an electrophoretically slow protein product, which earlier attracted attention as likely positively selected in harsh climate conditions. In PsbA-trnH, a 8bp deletion was found, which marks cultivated representatives of P. sativum.
authors.affiliation: Institute of Cytology and Genetics SB RAS, Acad. Lavrentyev ave. 10, Novosibirsk, Russia.
authors.forename: Olga O
authors.initials: OO
authors.lastname: Zaytseva
authors.forename: Vera S
authors.initials: VS
authors.lastname: Bogdanova
authors.forename: Oleg E
authors.initials: OE
authors.lastname: Kosterin
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D006657:Histones / Q000235:genetics*; D018532:Pisum sativum / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D006657:Histones