title: Analysis of variola and vaccinia virus neutralization assays for smallpox vaccines.
abstract: Possible smallpox reemergence drives research for third-generation vaccines that effectively neutralize variola virus. A comparison of neutralization assays using different substrates, variola and vaccinia (Dryvax and modified vaccinia Ankara [MVA]), showed significantly different 90% neutralization titers; Dryvax underestimated while MVA overestimated variola neutralization. Third-generation vaccines may rely upon neutralization as a correlate of protection.
authors.affiliation: National Center for Emerging and Zoonotic Infectious Diseases, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. Christine.Hughes@cdc.hhs.gov
authors.forename: Christine M
authors.initials: CM
authors.lastname: Hughes
authors.forename: Frances K
authors.initials: FK
authors.lastname: Newman
authors.forename: Whitni B
authors.initials: WB
authors.lastname: Davidson
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Olson
authors.forename: Scott K
authors.initials: SK
authors.lastname: Smith
authors.forename: Robert C
authors.initials: RC
authors.lastname: Holman
authors.forename: Lihan
authors.initials: L
authors.lastname: Yan
authors.forename: Sharon E
authors.initials: SE
authors.lastname: Frey
authors.forename: Robert B
authors.initials: RB
authors.lastname: Belshe
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Karem
authors.forename: Inger K
authors.initials: IK
authors.lastname: Damon
mesh_terms: D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D006801:Humans; D009500:Neutralization Tests / Q000379:methods*; D012900:Smallpox Vaccine / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology*; D012901:Variola virus / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D012900:Smallpox Vaccine