title: In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions.
abstract: The in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution that was lost upon deleting its PDZ binding TKF motif, suggesting that P5-1 interacts with cellular PDZ proteins. P5-2 located at the nucleus and its nuclear import was affected by the deletion of its basic C-termini. P7-1 and P7-2 also entered the nucleus and therefore, along with P5-2, could function as regulators of host gene expression. P6 located in the cytoplasm and in perinuclear cloud-like inclusions, was driven to P9-1 viroplasm-like structures and co-localized with P7-2, P10 and α-tubulin, suggesting its involvement in viroplasm formation and viral intracellular movement. Finally, P9-2 was N-glycosylated and located at the plasma membrane in association with filopodia-like protrusions containing actin, suggesting a possible role in virus cell-to-cell movement and spread.
authors.affiliation: Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (IB-INTA), Las cabañas y Los Reseros s/n. Hurlingham Cp 1686, Buenos Aires, Argentina.
authors.forename: Guillermo A
authors.initials: GA
authors.lastname: Maroniche
authors.forename: Vanesa C
authors.initials: VC
authors.lastname: Mongelli
authors.forename: Gabriela
authors.initials: G
authors.lastname: Llauger
authors.forename: Victoria
authors.initials: V
authors.lastname: Alfonso
authors.forename: Oscar
authors.initials: O
authors.lastname: Taboga
authors.forename: Mariana
authors.initials: M
authors.lastname: del Vas
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000821:virology; D002467:Cell Nucleus / Q000737:chemistry / Q000821:virology; D003593:Cytoplasm / Q000737:chemistry / Q000821:virology; D003599:Cytoskeleton / Q000821:virology; D016679:Genome, Viral; D049452:Green Fluorescent Proteins / Q000235:genetics; D018613:Microscopy, Confocal; D011993:Recombinant Fusion Proteins / Q000032:analysis; D012087:Reoviridae / Q000235:genetics / Q000502:physiology; D018411:Spodoptera / Q000648:ultrastructure / Q000821:virology*; D013347:Subcellular Fractions / Q000737:chemistry / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology*
chemical_list: D011993:Recombinant Fusion Proteins; D017361:Viral Nonstructural Proteins; D049452:Green Fluorescent Proteins