title: Suppressive effects of Schizandra chinensis Baillon water extract on allergy-related cytokine generation and degranulation in IgE-antigen complex-stimulated RBL-2H3 cells.
abstract: Schizandra chinensis Baillon is a traditional folk medicine plant that is used to treat and prevent several inflammatory diseases and cancer in Korea, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. This study was designed to investigate mechanisms of anti-allergic activity of a Schizandra chinensis Baillon water extract (SCWE) in immunoglobulin E (IgE)-antigen complex-stimulated RBL2H3 cells and to assess whether gastric and intestinal digestion affects the anti-allergic properties of SCWE. Oxidative stress is an important consequence of the allergic inflammatory response. The antioxidant activities of SCWE increased in a concentration-dependent manner. RBL-2H3 cells were sensitized with monoclonal anti-dinitrophenol (DNP) specific IgE, treated with SCWE, and challenged with the antigen DNP-human serum albumin. SCWE inhibited β-hexosaminidase release and expression of interleukin (IL)-4, IL-13, and tumor necrosis factor-alpha (TNF-α) mRNA and protein in IgE-antigen complex-stimulated RBL2H3 cells. We found that digested SCWE fully maintained its antioxidant activity and anti-allergic activity against the IgE-antigen complex-induced activation of RBL-2H3 cells. SCWE may be useful for preventing allergic diseases, such as asthma. Thus, SCWE could be used as a natural functional ingredient for allergic diseases in the food and/or pharmaceutical industries.
authors.affiliation: The Nutraceutical Bio Brain Korea 21 Project Group, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Mi Ja
authors.initials: MJ
authors.lastname: Chung
authors.forename: Jeong-Mi
authors.initials: JM
authors.lastname: Kim
authors.forename: Sangchul
authors.initials: S
authors.lastname: Lee
authors.forename: Taewoo
authors.initials: T
authors.lastname: Kim
authors.forename: Daejung
authors.initials: D
authors.lastname: Kim
authors.forename: Jongmi
authors.initials: J
authors.lastname: Baek
authors.forename: Taehyuk
authors.initials: T
authors.lastname: Kim
authors.forename: Jaesung
authors.initials: J
authors.lastname: Lee
authors.forename: Kyoungkon
authors.initials: K
authors.lastname: Kim
authors.forename: Jin A
authors.initials: JA
authors.lastname: Yoon
authors.forename: Myeon
authors.initials: M
authors.lastname: Choe