title: Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles.
abstract: We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin α(v)β(3)) and MCF-7 cells (human breast cancer cells, express low level of integrin α(v)β(3)) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs (SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin α(v)β(3)-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs.
authors.affiliation: Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul, Republic of Korea.
authors.forename: Myung-Ik
authors.initials: MI
authors.lastname: Yoo
authors.forename: Yeong-Ju
authors.initials: YJ
authors.lastname: Seo
authors.forename: Kyu-Sil
authors.initials: KS
authors.lastname: Choi
authors.forename: Jeong Sook
authors.initials: JS
authors.lastname: Ha
authors.forename: Kyoungja
authors.initials: K
authors.lastname: Woo
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005290:Ferric Compounds / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010456:Peptides, Cyclic / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D010456:Peptides, Cyclic; C099250:cyclic (arginyl-glycyl-aspartyl-phenylalanyl-lysyl); C000499:ferric oxide