title: Cell surface proteome of the marine planctomycete Rhodopirellula baltica.
abstract: The surface proteome (surfaceome) of the marine planctomycete Rhodopirellula baltica SH1(T)  was studied using a biotinylation and a proteinase K approach combined with SDS-PAGE and mass spectrometry. 52 of the proteins identified in both approaches could be assigned to the group of potential surface proteins. Among them are some high molecular weight proteins, potentially involved in cell-cell attachment, that contain domains shown before to be typical for surface proteins like cadherin/dockerin domains, a bacterial adhesion domain or the fasciclin domain. The identification of proteins with enzymatic functions in the R. baltica surfaceome provides further clues for the suggestion that some degradative enzymes may be anchored onto the cell surface. YTV proteins, which have been earlier supposed to be components of the proteinaceous cell wall of R. baltica, were detected in the surface proteome. Additionally, 8 proteins with a novel protein structure combining a conserved type IV pilin/N-methylation domain and a planctomycete-typical DUF1559 domain were identified.
authors.affiliation: Institute for Microbiology, Ernst-Moritz-Arndt-University, Greifswald, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Voigt
authors.forename: Cao Xuan
authors.initials: CX
authors.lastname: Hieu
authors.forename: Kristina
authors.initials: K
authors.lastname: Hempel
authors.forename: Dörte
authors.initials: D
authors.lastname: Becher
authors.forename: Rabea
authors.initials: R
authors.lastname: Schlüter
authors.forename: Hanno
authors.initials: H
authors.lastname: Teeling
authors.forename: Frank Oliver
authors.initials: FO
authors.lastname: Glöckner
authors.forename: Rudolf
authors.initials: R
authors.lastname: Amann
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
authors.forename: Thomas
authors.initials: T
authors.lastname: Schweder
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D015815:Cell Adhesion Molecules; D002473:Cell Wall / Q000737:chemistry; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D061328:Planctomycetales / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D020543:Proteome
chemical_list: D001426:Bacterial Proteins; D015815:Cell Adhesion Molecules; D008565:Membrane Proteins; D020543:Proteome