title: The threshold length for fiber-induced acute pleural inflammation: shedding light on the early events in asbestos-induced mesothelioma.
abstract: Suspicion has been raised that high aspect ratio nanoparticles or nanofibers might possess asbestos-like pathogenicity. The pleural space is a specific target for disease in individuals exposed to asbestos and by implication of nanofibers. Pleural effects of fibers depends on fiber length, but the key threshold length beyond which adverse effects occur has never been identified till now because all asbestos and vitreous fiber samples are heterogeneously distributed in their length. Nanotechnology advantageously allows for highly defined length distribution of synthetically engineered fibers that enable for in-depth investigation of this threshold length. We utilized the ability to prepare silver nanofibers of five defined length classes to demonstrate a threshold fiber length for acute pleural inflammation. Nickel nanofibers and carbon nanotubes were then used to strengthen the relationship between fiber length and pleural inflammation. A method of intrapleural injection of nanofibers in female C57Bl/6 strain mice was used to deliver the fiber dose, and we then assessed the acute pleural inflammatory response. Chest wall sections were examined by light and scanning electron microscopy to identify areas of lesion; furthermore, cell-nanowires interaction on the mesothelial surface of the parietal pleura in vivo was investigated. Our results showed a clear threshold effect, demonstrating that fibers beyond 4 µm in length are pathogenic to the pleura. The identification of the threshold length for nanofiber-induced pathogenicity in the pleura has important implications for understanding the structure-toxicity relationship for asbestos-induced mesothelioma and consequent risk assessment with the aim to contribute to the engineering of synthetic nanofibers by the adoption of a benign-by-design approach.
authors.affiliation: MRC/University of Edinburgh, Centre for Inflammation Research, Queen's Medical Research Institute,47 Little France Crescent, Edinburgh EH16 4TJ, United Kingdom.
authors.forename: Anja
authors.initials: A
authors.lastname: Schinwald
authors.forename: Fiona A
authors.initials: FA
authors.lastname: Murphy
authors.forename: Adriele
authors.initials: A
authors.lastname: Prina-Mello
authors.forename: Craig A
authors.initials: CA
authors.lastname: Poland
authors.forename: Fiona
authors.initials: F
authors.lastname: Byrne
authors.forename: Dania
authors.initials: D
authors.lastname: Movia
authors.forename: James R
authors.initials: JR
authors.lastname: Glass
authors.forename: Janet C
authors.initials: JC
authors.lastname: Dickerson
authors.forename: David A
authors.initials: DA
authors.lastname: Schultz
authors.forename: Chris E
authors.initials: CE
authors.lastname: Jeffree
authors.forename: William
authors.initials: W
authors.lastname: Macnee
authors.forename: Ken
authors.initials: K
authors.lastname: Donaldson
mesh_terms: D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D005260:Female; D008654:Mesothelioma / Q000139:chemically induced*; D008670:Metals / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000633:toxicity*; D010587:Phagocytosis; D010998:Pleurisy / Q000139:chemically induced*
chemical_list: D008670:Metals; D001194:Asbestos