title: [Effects of diazoxide on the mitochondrial membrane potential and ROS generation in rat uterus cells].
abstract: In the present study we demonstrate partial depolarization of the mitochondrial inner membrane from the rat uterus cells upon activation of mitochondrial ATP-sensitive K(+)-channel (mitoK(ATP)) with diazoxide. The estimated affinity constant of diazoxide to mitoK(ATP) from rat uterus cells is (5.01 +/- 1.47) 10(-6) M. We also observed an enhanced generation of reactive oxygen species after addition of diazoxide. Both effects were effectively eliminated by glybenclamide, blocker of the ATP-sensitive K+ channel. Our results indicate that activation of mitoK(ATP) in rat uterus cells leads to a partial depolarization of mitochondrial membrane and an increase in ROS concentration.
authors.forename: O B
authors.initials: OB
authors.lastname: Vadziuk
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003981:Diazoxide / Q000494:pharmacology*; D005260:Female; D005905:Glyburide / Q000494:pharmacology; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007700:Kinetics; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000502:physiology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism*; D032342:Muscle Cells / Q000187:drug effects / Q000378:metabolism*; D015221:Potassium Channels / Q000819:agonists / Q000378:metabolism*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D014599:Uterus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D007004:Hypoglycemic Agents; D015221:Potassium Channels; D017382:Reactive Oxygen Species; D014665:Vasodilator Agents; C117780:mitochondrial K(ATP) channel; D000255:Adenosine Triphosphate; D003981:Diazoxide; D005905:Glyburide