title: Preparation of biocompatible chitosan grafted poly(lactic acid) nanoparticles.
abstract: Chitosan grafted poly(lactic acid) (CS-g-PLA) copolymer was synthesized and characterized by FT-IR and elemental analysis. The degree of poly(lactic acid) substitution on chitosan was 1.90 ± 0.04%. The critical aggregation concentration of CS-g-PLA in distilled water was 0.17 mg/ml. Three methods of preparing CS-g-PLA nanoparticles (diafiltration method, ultrasonication method and diafiltration combined with ultrasonication method) were investigated and their effect was compared. Of the three methods, diafiltration combined with ultrasonication method produced nanoparticles with optimal property in terms of size and morphology, with size ranging from 133 to 352 nm and zeta potential from 36 to 43 mV. Also, the hemolytic activity and cytotoxicity of the CS-g-PLA based nanoparticles was tested, and results showed low hemolysis rate (<5%) and no significant cytotoxicity effect of these nanoparticles.
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao, 266003, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Kong
authors.forename: Xiao Jie
authors.initials: XJ
authors.lastname: Cheng
authors.forename: Qi Feng
authors.initials: QF
authors.lastname: Dang
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Ya Nan
authors.initials: YN
authors.lastname: Wei
authors.forename: Xi Guang
authors.initials: XG
authors.lastname: Chen
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology / Q000633:toxicity; D002460:Cell Line; D048271:Chitosan / Q000138:chemical synthesis*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D019344:Lactic Acid / Q000138:chemical synthesis*; D053758:Nanoparticles / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan