title: Characterisation of alleles of tomato light signalling genes generated by TILLING.
abstract: Targeting Induced Local Lesions IN Genomes (TILLING) combines chemical mutagenesis with high throughput screening to allow the generation of alleles of selected genes. In this study, TILLING has been applied to produce a series of mutations in genes encoding essential components of the tomato light signal transduction pathway in an attempt to enhance fruit nutritional quality. Point mutations to DEETIOLATED1 (DET1), which is responsible for the high pigment2 (hp2) tomato mutant, resulted in elevated levels of both carotenoid and phenylpropanoid phytonutrients in ripe fruit, whilst immature fruit showed increased chlorophyll content, photosynthetic capacity and altered fruit morphology. Furthermore, genotypes with mutations to the UV-DAMAGED DNA BINDING PROTEIN 1 (DDB1), COP1 and COP1like were also characterised. These genotypes largely did not display phenotypes characteristic of mutation to light signalling components but their characterisation has enabled interrogation of structure function relationships of the mutated genes.
authors.affiliation: School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom.
authors.forename: Matthew O
authors.initials: MO
authors.lastname: Jones
authors.forename: Florence
authors.initials: F
authors.lastname: Piron-Prunier
authors.forename: Fabien
authors.initials: F
authors.lastname: Marcel
authors.forename: Elodie
authors.initials: E
authors.lastname: Piednoir-Barbeau
authors.forename: Abdullah A
authors.initials: AA
authors.lastname: Alsadon
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Wahb-Allah
authors.forename: Abdullah A
authors.initials: AA
authors.lastname: Al-Doss
authors.forename: Chris
authors.initials: C
authors.lastname: Bowler
authors.forename: Peter M
authors.initials: PM
authors.lastname: Bramley
authors.forename: Paul D
authors.initials: PD
authors.lastname: Fraser
authors.forename: Abdelhafid
authors.initials: A
authors.lastname: Bendahmane
mesh_terms: D000483:Alleles; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005638:Fruit / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D017343:Genes, Plant / Q000235:genetics*; D023281:Genomics; D057166:High-Throughput Screening Assays / Q000379:methods*; D008027:Light; D018551:Solanum lycopersicum / Q000166:cytology / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D016296:Mutagenesis / Q000187:drug effects*; D010636:Phenols / Q000378:metabolism; D010788:Photosynthesis / Q000235:genetics / Q000528:radiation effects; D017354:Point Mutation; D025341:Principal Component Analysis; D015398:Signal Transduction / Q000235:genetics* / Q000528:radiation effects*
chemical_list: D010636:Phenols; D002734:Chlorophyll; D002338:Carotenoids; D005020:Ethyl Methanesulfonate