title: Paraoxonase-1 activity and oxidative status in patients with knee osteoarthritis and their relationship with radiological and clinical parameters.
abstract: BACKGROUND
  The aim of this study was to investigate serum paraoxonase-1 (PON1) activity and oxidative/anti-oxidative status in knee osteoarthritis (OA), and evaluate their relationship using radiological and clinical parameters.
  
  
  METHODS
  The study population comprised 127 patients with knee OA and 107 healthy volunteers. Patients with knee OA were divided into four subgroups according to the Kellgren-Lawrence (K&L) grading scale. In addition, each patient was clinically evaluated by the Western Ontario and McMaster University Osteoarthritis Index (WOMAC). Serum PON1 activity was measured spectrophotometrically. Oxidative status was assessed by measuring serum lipid hydroperoxide (LOOH) and total oxidant status (TOS). Anti-oxidative status was assessed by measuring serum free sulfydryl groups (-SH = total thiol) and total antioxidant capacity (TAC). Oxidative stress index (OSI) was calculated. Lipid parameters were determined by routine laboratory methods.
  
  
  RESULTS
  Serum PON1 activity was significantly lower in the knee OA group compared to the control group (p < 0.001), whereas serum LOOH, TOS, and OSI levels of the knee OA group were significantly higher than those of the controls (p < 0.001 for all). However, TAC and -SH levels did not differ between the two groups (p > 0.05). The lowest and highest mean serum PON1 activities were detected in patients with grades 4 and 1, respectively (ANOVA p < 0.001). In multiple regression analysis, WOMAC score was independently associated with serum PON1 activity (β = -0.248, p = 0.027).
  
  
  CONCLUSIONS
  Decreased serum PON1 activity and elevated LOOH, TOS, and OSI levels may be associated with knee OA, and serum PON1 activity may be a useful adjunctive indicator of the severity of knee OA for follow-up.
authors.affiliation: Department of Orthopaedics and Traumatology, Harran University School of Medicine, Sanliurfa, Turkey. erturkc@yahoo.com
authors.forename: Cemil
authors.initials: C
authors.lastname: Ertürk
authors.forename: Mehmet Akif
authors.initials: MA
authors.lastname: Altay
authors.forename: Sahbettin
authors.initials: S
authors.lastname: Selek
authors.forename: Abdurrahim
authors.initials: A
authors.lastname: Koçyiğit
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D000975:Antioxidants / Q000378:metabolism; D043303:Aryldialkylphosphatase / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000097:blood* / Q000000981:diagnostic imaging / Q000201:enzymology / Q000473:pathology; D018384:Oxidative Stress; D011859:Radiography; D012044:Regression Analysis; D012720:Severity of Illness Index; D013438:Sulfhydryl Compounds / Q000097:blood*
chemical_list: D000975:Antioxidants; D013438:Sulfhydryl Compounds; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human