title: [Protective effect of ALA on high glucose induced cellular injury of LLC-PK1 cell].
abstract: OBJECTIVE
  Made LLC-PK1 damage model induced by high glucose and observing the protection effect and its mechanisms of LLC-PK1 injury induced by high glucose.
  
  
  METHODS
  The proliferation of LLC-PK1 induced by high glucose was tested by CCK-8 and the apoptosis rat and the contents of reactive oxygen species (ROS) of LLC-PK1 damaged by high glucose was observed by flow cytometry after administration of different concentration alpha-linolenic acid (ALA).
  
  
  RESULTS
  High glucose could obviously inhibit the proliferation of LLC-PK1 The apoptotic rates of LLC-PK1 intervened by ALA (50-100 micromol/L) in the preconditioning group and the persistent intervention group were lower than those in the positive control group (P < 0.05). The contents of ROS of LLC-PK1 in the persistent intervention group were lower than those in the positive control group when the concentration of ALA were from 10 micromol/L to 100 micromol/L (P < 0.05, P < 0.01). The contents of ROS of LLC-PK1 in the preconditioning group were lower than those in the positive control group when the concentration of ALA was 50 micromol/L (P < 0.05).
  
  
  CONCLUSIONS
  The model of LLC-PK1 induced by high glucose provided fine chances for the intervention of renal tubular epithelial cells in DN. ALA were expected to be a protectant to prevent high glucose damage of renal tubulars. Decreasing the active oxygen generation may be one of the mechanism of the protective effects on LLC-PK1 by ALA.
authors.affiliation: School of Public Health, Southeast University, Nanjing 210009, China. snowbabyjmx@163.com
authors.forename: Mingxia
authors.initials: M
authors.lastname: Jiang
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Guilan
authors.initials: G
authors.lastname: Lv
authors.forename: Qi
authors.initials: Q
authors.lastname: Xu
authors.forename: Yinan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Chengkai
authors.initials: C
authors.lastname: Zhai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D003928:Diabetic Nephropathies / Q000517:prevention & control; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology; D007687:Kidney Tubules, Proximal / Q000166:cytology*; D020011:Protective Agents / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013552:Swine; D017962:alpha-Linolenic Acid / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D017382:Reactive Oxygen Species; D017962:alpha-Linolenic Acid; D005947:Glucose