title: Development of a broad-specificity monoclonal antibody-based immunoaffinity chromatography cleanup for organophosphorus pesticide determination in environmental samples.
abstract: An immunoaffinity chromatographic (IAC) method for the selective extraction and concentration of 13 organophosphorus pesticides (OPs, including coumaphos, parathion, phoxim, quinalphos, dichlofenthion, triazophos, azinphos-ethyl, phosalone, isochlorthion, parathion-methyl, cyanophos, disulfoton, and phorate) prior to analysis by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The IAC column was prepared by covalently immobilizing a monoclonal antibody with broad specificity for OPs on CNBr-activated Sephrose 4B. The column capacity ranged from 884 to 2641 ng/mL of gel. The optimum elution solvent was 0.01 M phosphate-buffered saline containing 80% methanol. The breakthrough volume of the IAC column was found to be 400 mL. Recoveries of OPs from spiked environmental samples by IAC cleanup and HPLC-MS/MS analysis ranged from 60.2 to 107.1%, with a relative standard deviation below 11.1%. The limit of quantitation for 13 OPs ranged from 0.01 to 0.13 ng/mL (ng/g). The application of IAC cleanup coupled to HPLC-MS/MS in real environmental samples demonstrated the potential of this method for the determination of OP residues in environmental samples at trace levels.
authors.affiliation: Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University , Guangzhou 510642, People's Republic of China.
authors.forename: Zhen-Lin
authors.initials: ZL
authors.lastname: Xu
authors.forename: Hao
authors.initials: H
authors.lastname: Deng
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Lei
authors.forename: Yue-Ming
authors.initials: YM
authors.lastname: Jiang
authors.forename: Katrina
authors.initials: K
authors.lastname: Campbell
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Shen
authors.forename: Jin-Yi
authors.initials: JY
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Sun
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods*; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D009943:Organophosphorus Compounds; D010575:Pesticides; D014874:Water Pollutants, Chemical