title: Antibacterial activity of extracts from five medicinal plants and their formula against bacteria that cause chronic wound infection.
abstract: BACKGROUND
  Chronic wound is caused by various factors such as chemotherapy, gene damage, treatment with steroids, diabetes mellitus, renal failure, blood pressure, infection and nutritional factors. One of the most common causes is bacterial infection. Antibacterial activity of several herbal plants has been reported. Thai medicinal plants which possess biological activities are potential to develop an alternative treatment of bacterial infection.
  
  
  OBJECTIVE
  To study efficiency of extracts from medicinal plants and their formula against bacteria that cause chronic wound infection.
  
  
  METHODS
  Extraction of Thai medicinal plants including Curcuma longa Linn, Rhinacanthus nasutus Linn, Garcinia mangostana Linn, Caesalpinia sappan Linn and Centellia asiatica Linn was performed by maceration with 95% ethanol and decoction followed by freeze dry. Formulation was conducted by varying the ratio of each components. Antibacterial activity were determined disk diffusion and broth dilution against Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Acinetobacter baumanii, Escherichia coli and Klebsiella pneumoniae.
  
  
  RESULTS
  Ethanolic extracts exhibited better antibacterial activity against tested strains than water extracts. Antibacterial activity of Caesalpinia sappan Linn. against S. aureus and MRSA showed the most effective with MIC value of 0.625 mg/ml. One of the five different formulas which contained two times proportion of C. sappan revealed that this formula was able to inhibit all tested strains with the MIC ranging between 0.156 mg/ml and 10 mg/ml.
  
  
  CONCLUSIONS
  C. sappan is the most effective herbal plant. The formula with two times proportion of C. sappan is potentially best formula for development of medicinal product of chronic wound infection. The potential active compound of C. sappan is suggested for further investigation of antimicrobial activity and other biological properties.
authors.affiliation: Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
authors.forename: Pornthep
authors.initials: P
authors.lastname: Temrangsee
authors.forename: Sumalee
authors.initials: S
authors.lastname: Kondo
authors.forename: Arunporn
authors.initials: A
authors.lastname: Itharat
mesh_terms: D029911:Caesalpinia; D028041:Centella; D030024:Curcuma; D004755:Enterobacteriaceae / Q000187:drug effects*; D029762:Garcinia mangostana; D016946:Gram-Negative Aerobic Rods and Cocci / Q000187:drug effects*; D006801:Humans; D031660:Jasminum; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D013211:Staphylococcus aureus / Q000187:drug effects*; D013785:Thailand; D014946:Wound Infection / Q000382:microbiology*
chemical_list: D010936:Plant Extracts