title: Development of a high-speed, multiplexed sample-delivery instrument for LC-MS/MS bioanalysis.
abstract: BACKGROUND
  The number of new chemical entities and types of in vitro and in vivo samples that require bioanalysis in drug discovery is large and diverse. In addition, method development time is limited as data turnaround is the highest priority. These circumstances require that a well-defined set of bioanalysis options be available in short timeframes to triage samples for analysis.
  
  
  METHODS
  The Apricot Designs Dual Arm (ADDA) instrument is an LC-MS/MS sample delivery system that features a flexible hardware design coupled with software automation to enhance throughput in LC-MS/MS bioanalysis drug discovery. The instrument can perform high-throughput LC-MS/MS (8-10 s/sample) for screening and in vitro bioanalysis, as well as multiplexed LC for traditional gradient or isocratic LC approaches. The instrument control software is designed to integrate with DiscoveryQuant™ software (AB Sciex) and a global database of MS/MS conditions.
  
  
  CONCLUSIONS
  Development of the sample delivery platform and its application in high-throughput and gradient LC will be described.
authors.affiliation: Pfizer Inc., Eastern Point Road, Groton, CT 06340, USA. john.s.janiszewski@pfizer.com
authors.forename: John
authors.initials: J
authors.lastname: Janiszewski
authors.forename: Richard
authors.initials: R
authors.lastname: Schneider
authors.forename: Brendon
authors.initials: B
authors.lastname: Kapinos
authors.forename: Mike
authors.initials: M
authors.lastname: West
authors.forename: Carrie
authors.initials: C
authors.lastname: Funk
authors.forename: James
authors.initials: J
authors.lastname: Federico
authors.forename: John
authors.initials: J
authors.lastname: Umland
authors.forename: Mary
authors.initials: M
authors.lastname: Piotrowski
authors.forename: Walter
authors.initials: W
authors.lastname: Mitchell
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Veronica
authors.initials: V
authors.lastname: Zelesky
authors.forename: Jason
authors.initials: J
authors.lastname: Gobey
authors.forename: Kevin
authors.initials: K
authors.lastname: Whalen
authors.forename: Theodore
authors.initials: T
authors.lastname: Liston
authors.forename: Matthew
authors.initials: M
authors.lastname: Troutman
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D004347:Drug Interactions; D057166:High-Throughput Screening Assays; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis*
chemical_list: D004364:Pharmaceutical Preparations