title: Sphingobacterium hotanense sp. nov., isolated from soil of a Populus euphratica forest, and emended descriptions of Sphingobacterium daejeonense and Sphingobacterium shayense.
abstract: A novel Gram-staining-negative bacterial strain, designated XH4(T), was isolated from soil of a Populus euphratica forest in the Hotan River valley, Xinjiang Uyghur autonomous region, PR China. The cells were strictly aerobic, non-motile, short rods. The isolate grew optimally at 37 °C and at pH 7.0-8.0. Based on 16S rRNA gene sequence analysis, strain XH4(T) belonged to the genus Sphingobacterium and was closely related to Sphingobacterium mizutaii ATCC 33299(T) (96.1 % sequence similarity). The DNA G+C content was 41.2 mol%. The major polar lipid of strain XH4(T) was phosphatidylethanolamine, and several unidentified polar lipids were also present. Strain XH4(T) showed the typical chemotaxonomic features of the genus Sphingobacterium, with the presence of ceramide phosphorylethanolamine 2 (CerPE-2) as the major ceramide. The major cellular fatty acids of strain XH4(T) were iso-C15 : 0 (34.0 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 20.7 %) and iso-C17 : 0 3-OH (14.7 %). The predominant isoprenoid quinone was MK-7. On the basis of phenotypic data and phylogenetic inference, strain XH4(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium hotanense sp. nov. is proposed. The type strain is XH4(T) ( = NRRL B-59204(T)  = CCTCC AB 209007(T)). Emended descriptions of Sphingobacterium daejeonense and Sphingobacterium shayense are also given.
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xiao
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cheng
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Haiju
authors.initials: H
authors.lastname: Kuang
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Xiangru
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Khayir
authors.initials: K
authors.lastname: Yusup
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Maryam
authors.initials: M
authors.lastname: Hamdun
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Abaidulla
authors.initials: A
authors.lastname: Gulsimay
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Fang
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Erkin
authors.initials: E
authors.lastname: Rahman
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013109:Sphingomyelins / Q000032:analysis; D014197:Trees / Q000382:microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D013109:Sphingomyelins; D024482:Vitamin K 2; C060281:ceramide phosphoethanolamine; C483858:phosphatidylethanolamine; C062629:menaquinone 7