title: Genetic modification of mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSC) are currently considered the most promising type of adult stem cells for therapeutic applications, because they can be easily isolated from the bone marrow and other tissues, and manipulated for different applications. The genetic transformation of MSC using genes that enhance their homing ability, as well as their proliferation and survival capacities when transplanted to sites of injury, is an important alternative to improve MSC function, especially for tissue regeneration. This chapter describes protocols for the transformation of MSC using plasmid vectors by lipofection and electroporation, as well as retroviral vectors representing viral transformations.
authors.affiliation: Laboratório de Cardiologia Molecular e Celular, Instituto de Cardiologia do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Andréia Escosteguy
authors.initials: AE
authors.lastname: Vargas
authors.forename: Melissa Medeiros
authors.initials: MM
authors.lastname: Markoski
authors.forename: Andrés Delgado
authors.initials: AD
authors.lastname: Cañedo
authors.forename: Flávia Helena
authors.initials: FH
authors.lastname: da Silva
authors.forename: Nance Beyer
authors.initials: NB
authors.lastname: Nardi
mesh_terms: D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D014170:Transformation, Genetic / Q000235:genetics*