title: Characterization of a peptide domain within the GB virus C envelope glycoprotein (E2) that inhibits HIV replication.
abstract: GB virus C (GBV-C) infection is associated with prolonged survival in HIV-infected cohorts, and GBV-C E2 protein inhibits HIV entry when added to CD4+ T cells. To further characterize E2 effects on HIV replication, stably transfected Jurkat cell lines expressing GBV-C E2 or control sequences were infected with HIV and replication was measured. HIV replication (all 6 isolates studied) was inhibited in all cell lines expressing a region of 17 amino acids of GBV-C E2, but not in cell lines expressing E2 without this region. In contrast, mumps and yellow fever virus replication was not inhibited by E2 protein expression. Synthetic GBV-C E2 17mer peptides did not inhibit HIV replication unless they were fused to a tat-protein-transduction-domain (TAT) for cellular uptake. These data identify the region of GBV-C E2 protein involved in HIV inhibition, and suggest that this GBV-C E2 peptide must gain entry into the cell to inhibit HIV.
authors.affiliation: Department of Internal Medicine, Iowa City Veterans Affairs Medical Center and University of Iowa, IA 52242, USA. jinhua-xiang@iowa.edu
authors.forename: Jinhua
authors.initials: J
authors.lastname: Xiang
authors.forename: James H
authors.initials: JH
authors.lastname: McLinden
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Kaufman
authors.forename: Emma L
authors.initials: EL
authors.lastname: Mohr
authors.forename: Nirjal
authors.initials: N
authors.lastname: Bhattarai
authors.forename: Qing
authors.initials: Q
authors.lastname: Chang
authors.forename: Jack T
authors.initials: JT
authors.lastname: Stapleton
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology*; D029407:GB virus C / Q000235:genetics / Q000378:metabolism*; D006678:HIV / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D056265:Microbial Interactions; D017434:Protein Structure, Tertiary; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D019380:Anti-HIV Agents; D014759:Viral Envelope Proteins; C107619:glycoprotein E2, GB virus C