title: Two-dimensional blue native/SDS gel electrophoresis of multiprotein complexes.
abstract: The two-dimensional blue native/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D BN/SDS-PAGE) is a method of choice for the investigation of protein complexes. This highly resolvent separation method is unique in that it facilitates the identification of many protein complexes simultaneously. Because of its simplicity and suitability, the 2D BN/SDS-PAGE can be now applied to a wide range of organisms, including bacteria, viruses, yeasts, animals, and plants. Moreover, recent modifications have made it possible to apply this method to the study of whole protein complexes of an organism. Here, we propose protocols for the investigation of the whole complexome of bacteria as well as eukaryotic cells.
authors.affiliation: Laboratoire de Bactériologie, INSERM U853, Université Victor Segalen Bordeaux 2, Bordeaux Cedex, France.
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Lasserre
authors.forename: Armelle
authors.initials: A
authors.lastname: Ménard
mesh_terms: D002021:Buffers; D018938:Caco-2 Cells; D002457:Cell Extracts / Q000737:chemistry / Q000302:isolation & purification; D004396:Coloring Agents / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004926:Escherichia coli / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000302:isolation & purification; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification; D016480:Helicobacter pylori / Q000737:chemistry; D006801:Humans; D046912:Multiprotein Complexes / Q000737:chemistry / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D020836:Protein Structure, Quaternary; D059748:Proteolysis; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification; D012394:Rosaniline Dyes / Q000737:chemistry; D012835:Silver Nitrate / Q000737:chemistry; D016622:Silver Staining; D014357:Trypsin / Q000737:chemistry; D025062:Yarrowia / Q000737:chemistry
chemical_list: D002021:Buffers; D002457:Cell Extracts; D004396:Coloring Agents; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D046912:Multiprotein Complexes; D010446:Peptide Fragments; D020543:Proteome; D012394:Rosaniline Dyes; D012835:Silver Nitrate; D014357:Trypsin; C004692:coomassie Brilliant Blue