title: Frequency-dependent signal transfer at the interface between electrogenic cells and nanocavity electrodes.
abstract: We present a model to describe the response of chip-based nanocavity sensors during extracellular recording of action potentials. These sensors feature microelectrodes which are embedded in liquid-filled cavities. They can be used for the highly localized detection of electrical signals on a chip. We calculate the sensor's impedance and simulate the propagation of action potentials. Subsequently we apply our findings to analyze cell-chip coupling properties. The results are compared to experimental data obtained from cardiomyocyte-like cells. We show that both the impedance and the modeled action potentials fit the experimental data well. Furthermore, we find evidence for a large seal resistance of cardiomyocytes on nanocavity sensors compared to conventional planar recording systems.
authors.affiliation: Institute of Bioelectronics (PGI-8/ICS-8), Forschungszentrum Jülich GmbH, Jülich, Germany. mschottdorf@physik.uni-wuerzburg.de
authors.forename: Manuel
authors.initials: M
authors.lastname: Schottdorf
authors.forename: Boris
authors.initials: B
authors.lastname: Hofmann
authors.forename: Enno
authors.initials: E
authors.lastname: Kätelhön
authors.forename: Andreas
authors.initials: A
authors.lastname: Offenhäusser
authors.forename: Bernhard
authors.initials: B
authors.lastname: Wolfrum
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D003198:Computer Simulation; D017076:Computer-Aided Design; D017097:Electric Impedance; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008839:Microelectrodes; D008954:Models, Biological; D032383:Myocytes, Cardiac / Q000502:physiology*; D036103:Nanotechnology / Q000295:instrumentation*; D013499:Surface Properties