title: [Correlation between smoking and the polymorphisms of cytochrome P450 1A1-Msp I and glutathione S-transferase T1 genes and oral cancer].
abstract: OBJECTIVE
  To investigate the correlation between the combination of smoking and the polymorphisms of cytochrome P450 (CYP) 1A1-Msp I and glutathione S-transferase (GST) T1 genes and oral cancer.
  
  
  METHODS
  The genetic polymorphisms of CYP1A1-Msp I and GSTT1 were detected by polymerase chain reaction (PCR) technique in peripheral blood leukocytes of 300 oral cancer cases and 300 non-cancer controls, and the correlation between smoking, the two metabolic enzymes genetic polymorphisms and oral cancer were analyzed.
  
  
  RESULTS
  The frequencies of CYP1A1-Msp I (m2/m2) and GSTT1(-) were 38.33% and 69.33% in oral cancer cases and 21.00% and 44.33% in healthy controls respectively. Statistical tests showed significant difference in the frequencies between the two groups (P<0.01). The risk of oral cancer with CYP1A1-Msp I (m2/m2) was significantly higher than that of controls (OR= 2.34, 95%CI 1.76-4.07). The individuals who carried with GSTT1(-) had a high risk of oral cancer(OR=2.84, 95% CI 1.98-4.54). Combined analysis of the polymorphisms showed that percentage of CYP1A1-Msp I (m2/m2)/GSTT1(-) in oral cancer and control groups was 30.67% and 6.67% respectively (P<0.01). The people who carried with CYP1A1-Msp I (m2/m2)/GSTT1(-) had a high risk of oral cancer(OR=8.27, 95%CI 3.63-11.29). The smoking rate of the case group was significantly higher than that in the control group (OR=2.71, 95%CI 1.31-4.52, P<0.01), and statistic analysis suggested an interaction between smoking and CYP1A1-Msp I (m2/m2)/GSTT1(-) genotypes polymorphisms which increased risk of oral cancer (OR=25.00, 95%CI 11.87-35.64).
  
  
  CONCLUSIONS
  CYP1A1-Msp I (m2/m2) and GSTT1(-) are the risk factors in oral cancer. Smoking is also related to the susceptibility to oral cancer. There may be a synergetic interaction among CYP1A1-Msp I (m2/m2), GSTT1(-) and smoking on the elevated susceptibility of oral cancer.
authors.affiliation: Dept. of Stomatology, The First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, China.
authors.forename: Like
authors.initials: L
authors.lastname: Guo
authors.forename: Chaoxian
authors.initials: C
authors.lastname: Zhang
authors.forename: Shumin
authors.initials: S
authors.lastname: Shi
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Guo
mesh_terms: D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1; D005838:Genotype; D005982:Glutathione Transferase; D006801:Humans; D009062:Mouth Neoplasms; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012907:Smoking
chemical_list: D019363:Cytochrome P-450 CYP1A1; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase