title: Stability of peptide-HLA-I complexes and tapasin folding facilitation--tools to define immunogenic peptides.
abstract: Only a small fraction of the peptides generated inside the cell end up being presented by HLA-I on the cell surface. High stability of peptide-HLA-I complexes and a low HLA-I tapasin-facilitation have been proposed to predict immunogenicity. We here set out to investigate if these parameters correlated and defined immunogenic peptides. Both peptide-HLA-B(?)08:01 and peptide-HLA-A(?)02:01 complexes showed small differences in tapasin-facilitation and larger differences in stability. This suggests that the stability of immunogenic peptide-HLA-I complexes vary above an HLA-I allomorph dependent lower limit (e.g. >2h for HLA-A(?)02:01), immunogenicity predicted by tapasin-facilitation may be defined by an equally allomorph unique upper value (e.g. tapasin-facilitation <1.5 for HLA-A(?)02:01), and variation above the stability-threshold does not directly reflect a variation in tapasin-facilitation.
authors.affiliation: Immunology Section, BMC-D14, Lund University, Experimental Medical Sciences, Lund, Sweden.
authors.forename: Linda
authors.initials: L
authors.lastname: Geironson
authors.forename: Gustav
authors.initials: G
authors.lastname: Røder
authors.forename: Kajsa
authors.initials: K
authors.lastname: Paulsson
mesh_terms: D000595:Amino Acid Sequence; D015234:HLA-A Antigens / Q000276:immunology*; D015235:HLA-B Antigens / Q000276:immunology; D026901:Membrane Transport Proteins / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000276:immunology*; D017510:Protein Folding; D055550:Protein Stability
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D026901:Membrane Transport Proteins; D010455:Peptides; C107762:tapasin