title: Use of simple sequence repeat  markers for DNA fingerprinting and diversity analysis  of sugarcane (Saccharum spp) cultivars  resistant and susceptible to red rot.
abstract: Red rod  is an economically important disease of sugarcane caused by the fungus Colletotrichum  falcatum. We used a simple sequence repeat (SSR)-based marker system to  identify and analyze genetic relationships of red rot resistant and susceptible  sugarcane cultivars grown in Pakistan. Twenty-one highly polymorphic SSR  markers were used for DNA fingerprinting and genetic diversity analysis of 20  sugarcane cultivars. These SSR markers were found to be highly robust; we  identified 144 alleles, with 3-11 alleles per marker and a mean of 6.8. Three  SSR markers were able to identify all 20 cultivars. DNAMAN(®)-generated  homology tree was used to analyze genetic diversity among these cultivars; all  cultivars shared 58% or more similarity. We correlated polymorphism information  content and resolving power values with marker effectiveness in the process of  sugarcane cultivar identification. We concluded that a small number of  SSR-derived DNA markers will allow breeders to identify red rot resistant and  susceptible cultivars.
authors.affiliation: School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
authors.forename: U
authors.initials: U
authors.lastname: Hameed
authors.forename: Y-B
authors.initials: YB
authors.lastname: Pan
authors.forename: K
authors.initials: K
authors.lastname: Muhammad
authors.forename: S
authors.initials: S
authors.lastname: Afghan
authors.forename: J
authors.initials: J
authors.lastname: Iqbal
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers; D019075:Electrophoresis, Capillary; D006579:Heterozygote; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D031786:Saccharum / Q000235:genetics*
chemical_list: D017931:DNA Primers