title: Egg white-mediated green synthesis of silver nanoparticles with excellent biocompatibility and enhanced radiation effects on cancer cells.
abstract: A simple, cost-effective, and environmentally friendly approach to the aqueous-phase synthesis of silver (Ag) nanoparticles was demonstrated using silver nitrate (AgNO(3)) and freshly extracted egg white. The bio-conjugates were characterized by UV-visible spectroscopy, transmission electron microscopy, Fourier transform infrared spectrometry, and dynamic light scattering. These results indicated that biomolecule-coated Ag nanoparticles are predominantly spherical in shape with an average size of 20 nm. The proteins of egg white, which have different functional groups, played important roles in reducing Ag(+) and maintaining product attributes such as stability and dispersity. In vitro cytotoxicity assays showed that these Ag-protein bio-conjugates showed good biocompatibility with mouse fibroblast cell lines 3T3. Furthermore, X-ray irradiation tests on 231 tumor cells suggested that the biocompatible Ag-protein bio-conjugates enhanced the efficacy of irradiation, and thus may be promising candidates for use during cancer radiation therapy.
authors.affiliation: Department of Clinical Laboratory, Fudan University Shanghai Cancer Center, Shanghai, People’s Republic of China.
authors.forename: Renquan
authors.initials: R
authors.lastname: Lu
authors.forename: Dapeng
authors.initials: D
authors.lastname: Yang
authors.forename: Daxiang
authors.initials: D
authors.lastname: Cui
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001943:Breast Neoplasms / Q000532:radiotherapy; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D004529:Egg White; D005260:Female; D055772:Green Chemistry Technology; D006801:Humans; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D058726:Nanoconjugates / Q000008:administration & dosage / Q000737:chemistry; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage; D012834:Silver / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D058726:Nanoconjugates; D011838:Radiation-Sensitizing Agents; D012834:Silver