title: Characterization of imipenem resistance mechanisms in Pseudomonas aeruginosa isolates from Turkey.
abstract: The emergence of carbapenem resistance in Pseudomonas aeruginosa threatens the efficacy of this important anti-pseudomonal antibiotic class. Between 2003 and 2006, an increase in the number of carbapenem-resistant P. aeruginosa isolates at the Zonguldak Karaelmas University Hospital was observed (Zonguldak, Turkey). To assess the imipenem resistance mechanisms emerging in these P. aeruginosa isolates, they were characterized by amplified fragment length polymorphism typing, which revealed diversity among imipenem-resistant isolates as well as two clonally related outbreak groups. The molecular mechanism of carbapenem resistance was characterized in a representative isolate from each clonal group. Mutational disruption of oprD was the most frequently encountered resistance mechanism (23/27 isolates).
authors.affiliation: BIOMERIT Research Centre, Department of Microbiology, University College Cork, Cork, Ireland.
authors.forename: M
authors.initials: M
authors.lastname: Mac Aogáin
authors.forename: C
authors.initials: C
authors.lastname: Kulah
authors.forename: M
authors.initials: M
authors.lastname: Rijnsburger
authors.forename: G
authors.initials: G
authors.lastname: Celebi
authors.forename: P H M
authors.initials: PH
authors.lastname: Savelkoul
authors.forename: F
authors.initials: F
authors.lastname: O'Gara
authors.forename: M J
authors.initials: MJ
authors.lastname: Mooij
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D055786:Gene Knockout Techniques; D014644:Genetic Variation; D006785:Hospitals, University; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D018272:Porins / Q000235:genetics; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D014421:Turkey; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents; D018272:Porins; C077157:OprD protein, Pseudomonas aeruginosa; D015378:Imipenem