title: The role of CD98hc in mouse macrophage functions.
abstract: CD98hc is a type II transmembrane protein that covalently links to one of several L-type amino acid transporters. CD98hc was first identified as a lymphocyte activation marker. In this study, we examined the role that CD98hc plays in the functions of macrophages using tissue specific knock-out miceCD98hc (CD98hc(flox/-)LysM-cre mice). When isolated peritoneal macrophages were incubated for 48 h, the macrophages obtained from the knock-out mice showed round-shaped morphologies, while almost all of the cells obtained from the control mice were spindle-shaped. The macrophage functions such as the antigen-presenting, phagocytic, and fusion activities, have been reported to decrease in CD98hc-deficient peritoneal macrophages. In addition, when the CD98hc deficient macrophages were stimulated with either IFN-γ/LPS or IL-4, the production of NO(2) or arginase-I decreased in comparison to that observed in the control macrophages. These findings show that the CD98hc molecules play an important role in the activation and functions of macrophages.
authors.affiliation: Division of Laboratory Animal Resources, National Research Institute for Child Health and Development, Tokyo, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Tsumura
authors.forename: Morihiro
authors.initials: M
authors.lastname: Ito
authors.forename: Xiao-Kang
authors.initials: XK
authors.lastname: Li
authors.forename: Akane
authors.initials: A
authors.lastname: Nakamura
authors.forename: Naoko
authors.initials: N
authors.lastname: Ohnami
authors.forename: Jun-Ichiro
authors.initials: J
authors.lastname: Fujimoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Komada
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D027282:Fusion Regulatory Protein 1, Heavy Chain / Q000235:genetics / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D010587:Phagocytosis
chemical_list: D027282:Fusion Regulatory Protein 1, Heavy Chain; C523212:Slc3A2 protein, mouse