title: Isolation and characterization of microsatellite markers from Tiarella polyphylla (Saxifragaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for Tiarella polyphylla in Korea and Japan to study the genetic diversity and genetic structure of the species.
  
  
  RESULTS
  We successfully isolated a total of nine polymorphic simple sequence repeat loci. These nine loci contained from two to eight alleles per locus. The observed and expected heterozygosities ranged from 0.000 to 0.250 and 0.000 to 0.974, respectively.
  
  
  CONCLUSIONS
  The markers developed in this study may be used to estimate genetic diversity, genetic structure, and historical and recent gene flow in T. polyphylla, which requires conservation and management.
authors.affiliation: Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Keum Seon
authors.initials: KS
authors.lastname: Jeong
authors.forename: Masayuki
authors.initials: M
authors.lastname: Maki
authors.forename: Jae-Hong
authors.initials: JH
authors.lastname: Pak
mesh_terms: D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D031995:Saxifragaceae / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant