title: The human hepatocyte cell lines IHH and HepaRG: models to study glucose, lipid and lipoprotein metabolism.
abstract: Metabolic diseases reach epidemic proportions. A better knowledge of the associated alterations in the metabolic pathways in the liver is necessary. These studies need in vitro human cell models. Several human hepatoma models are used, but the response of many metabolic pathways to physiological stimuli is often lost. Here, we characterize two human hepatocyte cell lines, IHH and HepaRG, by analysing the expression and regulation of genes involved in glucose and lipid metabolism. Our results show that the glycolysis pathway is activated by glucose and insulin in both lines. Gluconeogenesis gene expression is induced by forskolin in IHH cells and inhibited by insulin in both cell lines. The lipogenic pathway is regulated by insulin in IHH cells. Finally, both cell lines secrete apolipoprotein B-containing lipoproteins, an effect promoted by increasing glucose concentrations. These two human cell lines are thus interesting models to study the regulation of glucose and lipid metabolism.
authors.affiliation: Univ Lille Nord de France, Lille, France.
authors.forename: Carolina Huaman
authors.initials: CH
authors.lastname: Samanez
authors.forename: Sandrine
authors.initials: S
authors.lastname: Caron
authors.forename: Olivier
authors.initials: O
authors.lastname: Briand
authors.forename: Hélène
authors.initials: H
authors.lastname: Dehondt
authors.forename: Isabelle
authors.initials: I
authors.lastname: Duplan
authors.forename: Folkert
authors.initials: F
authors.lastname: Kuipers
authors.forename: Nathalie
authors.initials: N
authors.lastname: Hennuyer
authors.forename: Véronique
authors.initials: V
authors.lastname: Clavey
authors.forename: Bart
authors.initials: B
authors.lastname: Staels
mesh_terms: D001055:Apolipoproteins B / Q000096:biosynthesis; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D005786:Gene Expression Regulation; D005943:Gluconeogenesis / Q000187:drug effects; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects; D008954:Models, Biological; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: D001055:Apolipoproteins B; D007328:Insulin; D034741:RNA, Small Interfering; D005576:Colforsin; D005947:Glucose