title: High-resolution ex vivo imaging in mouse spinal cord using micro-CT with 11.7T-MRI and myelin staining validation.
abstract: We investigated the use of micro-CT with contrast agent for the characterization of fixed mouse spinal cord as a means to differentiate between gray and white matter. The spinal cords were soaked in a concentration of nonionic iodinated contrast agent for 14 days. Micro-CT was performed and then compared using 11.7T-MRI images and myelin staining. Soaking the spinal cords in contrast agent resulted in clear differences in signal between the gray and white matter at 3 planes. Micro-CT provides more relevant information on mouse spinal cord GM and WM anatomical structures.
authors.affiliation: Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan. saito@sahs.med.osaka-u.ac.jp
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Saito
authors.forename: Yuki
authors.initials: Y
authors.lastname: Mori
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Kenya
authors.initials: K
authors.lastname: Murase
mesh_terms: D000818:Animals; D008279:Magnetic Resonance Imaging / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009413:Nerve Fibers, Myelinated; D059906:Neuroimaging / Q000379:methods*; D013116:Spinal Cord / Q000166:cytology*; D013194:Staining and Labeling; D055114:X-Ray Microtomography / Q000379:methods