title: A bacterial two-hybrid genome fragment library for deciphering regulatory networks of the opportunistic pathogen Pseudomonas aeruginosa.
abstract: Bacterial gene regulation is controlled by complex regulatory cascades which integrate input environmental signals and adapt specific and adequate output cellular responses. These complex networks are far from being elucidated, in particular in Pseudomonas aeruginosa. In the present study, we developed bacterial two-hybrid genome fragment libraries of the P. aeruginosa PAO1 strain to identify potential partners involved in the HptB/HsbR/HsbA pathway. This powerful tool, validated by the interaction previously described between HsbR and HsbA proteins, allowed us to demonstrate that the HsbR response regulator dimerizes through its PP2C/ATPase C-terminal effector domain, an observation further confirmed by pull-down experiments. This will also allow us to identify further new partners in this cascade.
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR7255 CNRS - Aix Marseille Université, 31 Chemin Joseph Aiguier, 13402 Marseille, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Houot
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR7255 CNRS - Aix Marseille Université, 31 Chemin Joseph Aiguier, 13402 Marseille, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Fanni
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR7255 CNRS - Aix Marseille Université, 31 Chemin Joseph Aiguier, 13402 Marseille, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: de Bentzmann
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR7255 CNRS - Aix Marseille Université, 31 Chemin Joseph Aiguier, 13402 Marseille, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Bordi
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D015698:Genomic Library; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins