title: Murine T cell-derived contractile activity for bronchial smooth muscle cells.
abstract: BACKGROUND
  Adoptive transfer of helper T (Th) cells conferred a late asthmatic response upon antigen challenge. A possible production of a contractile activity for bronchial smooth muscle (BSM) by activated Th cells was examined.
  
  
  METHODS
  Murine Th clones were stimulated with immobilized anti-CD3 antibody in the presence or absence of anti-CD28 antibody. Culture supernatants were dialyzed and then applied to the collagen gels containing cultured human BSM cells.
  
  
  RESULTS
  Culture supernatants of activated but not resting murine Th clones that conferred a late asthmatic response, induced the contraction of human BSM cell-containing collagen gels.
  
  
  CONCLUSIONS
  Activated Th cells produce a contractile activity for BSM in vitro.
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, Sagamihara, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Abe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Koyama
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ohtomo
authors.forename: Noriko
authors.initials: N
authors.lastname: Kitamura
authors.forename: Osamu
authors.initials: O
authors.lastname: Kaminuma
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
mesh_terms: D000818:Animals; D001980:Bronchi / Q000166:cytology / Q000502:physiology*; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D006801:Humans; D051379:Mice; D009119:Muscle Contraction; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D006377:T-Lymphocytes, Helper-Inducer / Q000502:physiology*
chemical_list: D018106:CD28 Antigens; D017252:CD3 Complex; D016207:Cytokines