title: Recombinant interferon-γ activates immune responses against Edwardsiella tarda infection in the olive flounder, Paralichthys olivaceus.
abstract: Interferon gamma (IFN-γ) is a cytokine that plays a very important role in defining Th1 immune response in all vertebrates. In this study, recombinant IFN-γ (rIFN-γ) from the olive flounder (Paralichthys olivaceus) was produced in an Escherichia coli system using a pET expression vector. Stimulation of whole kidney leukocytes (immune-related cells) in vitro with the resulting rIFN-γ significantly induced the gene expression of interleukin-1β (IL-1β), signal transducer and activator of transcription 1 (STAT1), CXCL13-like chemokine (CXCL13), and IFN-γ. rIFN-γ also weakly induced the expression of IL-1β, tumor necrosis factor-α (TNF-α), CXCL13, and IFN-γ in olive flounder-derived HINAE (non-immune) cells. The effects of rIFN-γ against Edwardsiella tarda infection in vivo were assessed by intraperitoneally injecting a mixture of rIFN-γ (100 ng) and E. tarda (1 × 10(5) CFU/ml) into the olive flounder. The survival rate in the rIFN-γ-injected group was 60% compared to 0% in the group treated with E. tarda only, demonstrating that olive flounder IFN-γ is effective in reinforcing immune responses and preventing against edwardsiellosis.
authors.affiliation: Aquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongnam, South Korea.
authors.forename: Chan Yong
authors.initials: CY
authors.lastname: Jung
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Hikima
authors.forename: Maki
authors.initials: M
authors.lastname: Ohtani
authors.forename: Ho Bin
authors.initials: HB
authors.lastname: Jang
authors.forename: Carmelo S
authors.initials: CS
authors.lastname: del Castillo
authors.forename: Seong Won
authors.initials: SW
authors.lastname: Nho
authors.forename: In Seok
authors.initials: IS
authors.lastname: Cha
authors.forename: Seong Bin
authors.initials: SB
authors.lastname: Park
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Jung
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D020609:Edwardsiella tarda / Q000276:immunology; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000401:mortality / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology* / Q000401:mortality; D005432:Flounder / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D007962:Leukocytes / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology* / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D011994:Recombinant Proteins; D007371:Interferon-gamma