title: A selective Seoul-Fluor-based bioprobe, SfBP, for vaccinia H1-related phosphatase--a dual-specific protein tyrosine phosphatase.
abstract: We report a Seoul-Fluor-based bioprobe, SfBP, for selective monitoring of protein tyrosine phosphatases (PTPs). A rational design based on the structures at the active site of dual-specific PTPs can enable SfBP to selectively monitor the activity of these PTPs with a 93-fold change in brightness. Moreover, screening results of SfBP against 30 classical PTPs and 35 dual-specific PTPs show that it is selective toward vaccinia H1-related (VHR) phosphatase, a dual-specific PTP (DUSP-3).
authors.affiliation: BioNanotechnology Research Center, KRIBB and NanoBio Major, UST, 111 Kwahangno, Yuseong, Daejeon 305-806, Korea.
authors.forename: Myeong Seon
authors.initials: MS
authors.lastname: Jeong
authors.forename: Eunha
authors.initials: E
authors.lastname: Kim
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Kang
authors.forename: Eun Joung
authors.initials: EJ
authors.lastname: Choi
authors.forename: Alvin R
authors.initials: AR
authors.lastname: Cho
authors.forename: Sang J
authors.initials: SJ
authors.lastname: Chung
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Park
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D054640:Dual Specificity Phosphatase 3 / Q000378:metabolism*; D004579:Electron Transport; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D010766:Phosphorylation; D012680:Sensitivity and Specificity; D014615:Vaccinia / Q000201:enzymology / Q000821:virology; D014616:Vaccinia virus / Q000201:enzymology*
chemical_list: D005456:Fluorescent Dyes; D054640:Dual Specificity Phosphatase 3