title: Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway.
abstract: In Saccharomyces cerevisiae, the transcriptional program triggered by cell wall stress is coordinated by Slt2/Mpk1, the mitogen-activated protein kinase (MAPK) of the cell wall integrity (CWI) pathway, and is mostly mediated by the transcription factor Rlm1. Here we show that the SWI/SNF chromatin-remodeling complex plays a critical role in orchestrating the transcriptional response regulated by Rlm1. swi/snf mutants show drastically reduced expression of cell wall stress-responsive genes and hypersensitivity to cell wall-interfering compounds. On stress, binding of RNA Pol II to the promoters of these genes depends on Rlm1, Slt2, and SWI/SNF. Rlm1 physically interacts with SWI/SNF to direct its association to target promoters. Finally, we observe nucleosome displacement at the CWI-responsive gene MLP1/KDX1, which relies on the SWI/SNF complex. Taken together, our results identify the SWI/SNF complex as a key element of the CWI MAPK pathway that mediates the chromatin remodeling necessary for adequate transcriptional response to cell wall stress.
authors.affiliation: Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: A Belén
authors.initials: AB
authors.lastname: Sanz
authors.forename: Raúl
authors.initials: R
authors.lastname: García
authors.forename: Jose Manuel
authors.initials: JM
authors.lastname: Rodríguez-Peña
authors.forename: Sonia
authors.initials: S
authors.lastname: Díez-Muñiz
authors.forename: César
authors.initials: C
authors.lastname: Nombela
authors.forename: Craig L
authors.initials: CL
authors.lastname: Peterson
authors.forename: Javier
authors.initials: J
authors.lastname: Arroyo
mesh_terms: D002473:Cell Wall / Q000235:genetics / Q000502:physiology*; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism; D016601:RNA-Binding Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D026161:MADS Domain Proteins; C080988:MLP1 protein, S cerevisiae; D009687:Nuclear Proteins; C095725:RLM1 protein, S cerevisiae; D016601:RNA-Binding Proteins; C445179:SWI-SNF-B chromatin-remodeling complex; C078562:SWI1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D020928:Mitogen-Activated Protein Kinases; C072724:SLT2 protein, S cerevisiae; D012319:RNA Polymerase II