title: Distinguishing xyloglucanase activity in endo-β(1→4)glucanases.
abstract: The ability of β-glucanases to cleave xyloglucans, a family of highly decorated β-glucans ubiquitous in plant biomass, has traditionally been overlooked in functional biochemical studies. An emerging body of data indicates, however, that a spectrum of xyloglucan specificity resides in diverse glycoside hydrolases from a range of carbohydrate-active enzyme families-including classic "cellulase" families. This chapter outlines a series of enzyme kinetic and product analysis methods to establish degrees of xyloglucan specificity and modes of action of glycosidases emerging from enzyme discovery projects.
authors.affiliation: Michael Smith Laboratories and Department of Chemistry, University of British Columbia, Vancouver, Canada.
authors.forename: Jens M
authors.initials: JM
authors.lastname: Eklöf
authors.forename: Marcus C
authors.initials: MC
authors.lastname: Ruda
authors.forename: Harry
authors.initials: H
authors.lastname: Brumer
mesh_terms: D002240:Carbohydrate Sequence; D002480:Cellulase / Q000378:metabolism*; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D057075:Enzyme Assays / Q000379:methods*; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D010944:Plants / Q000737:chemistry; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology; D014990:Xylans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D005936:Glucans; D014990:Xylans; C029353:xyloglucan; D002480:Cellulase