title: Microplate assay for screening the antibacterial activity of Schiff bases derived from substituted benzopyran-4-one.
abstract: Schiff bases (SB(1)-SB(3)) were synthesized from the condensation of 6-formyl-7-hydroxy-5-methoxy-2-methylbenzopyran-4-one with 2-aminopyridine (SB(1)), p-phenylenediamine (SB(2)) and o-phenylenediamine (SB(3)), while Schiff bases (SB(4)-SB(6)) were synthesized by condensation of 5,7-dihydroxy-6-formyl-2-methylbenzopyran-4-one with 2-aminopyridine (SB(4)), p-phenylenediamine (SB(5)) and o-phenylenediamine (SB(6)). Schiff bases were characterized using elemental analysis, IR, UV-Vis, (1)H NMR, (13)C NMR and mass spectroscopy. These compounds were screened for antibacterial activities by micro-plate assay technique. Escherichia coli and Staphylococcus capitis were exposed to different concentrations of the Schiff bases. Results showed that the antibacterial effect of these Schiff bases on Gram-negative bacteria were higher than that on Gram-positive bacteria moreover, the Schiff bases containing substituent OCH(3) on position five have higher antibacterial activity than that containing hydroxy group on the same position.
authors.affiliation: National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt. rehabamin@niles.edu.eg
authors.forename: Rehab M
authors.initials: RM
authors.lastname: Amin
authors.forename: Nora S
authors.initials: NS
authors.lastname: Abdel-Kader
authors.forename: Aida L
authors.initials: AL
authors.lastname: El-Ansary
mesh_terms: D000042:Absorption / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001578:Benzopyrans / Q000737:chemistry*; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods*; D004126:Dimethylformamide / Q000737:chemistry; D004583:Electrons; D004926:Escherichia coli / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests / Q000295:instrumentation* / Q000379:methods*; D012545:Schiff Bases / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013210:Staphylococcus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001578:Benzopyrans; D012545:Schiff Bases; C575677:benzopyran-4-one; D004126:Dimethylformamide