title: A Foodomics approach: CE-MS for comparative metabolomics of colon cancer cells treated with dietary polyphenols.
abstract: The potential of capillary electrophoresis-mass spectrometry (CE-MS) for metabolomics is demonstrated through the analysis of metabolites from human HT29 colon cancer cells treated and non-treated with dietary polyphenols. Prior to CE-MS analysis, four different metabolite purification strategies are investigated. Namely, the results obtained after methanol deproteinization, ultrafiltration, and two solid-phase extraction methods using C18 and polymer-based cartridges are described. These generic methods can have broad applications to analyze metabolites in a large variety of matrices and fields, including the new Foodomics area.
authors.affiliation: Laboratory of Foodomics, CIAL, CSIC, Madrid, Spain.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Celebier
authors.forename: Clara
authors.initials: C
authors.lastname: Ibáñez
authors.forename: Carolina
authors.initials: C
authors.lastname: Simó
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cifuentes
mesh_terms: D002457:Cell Extracts / Q000737:chemistry / Q000302:isolation & purification; D003110:Colonic Neoplasms; D019075:Electrophoresis, Capillary / Q000379:methods*; D005502:Food; D005590:Fractional Precipitation; D019073:HT29 Cells; D006801:Humans; D055442:Metabolome; D055432:Metabolomics; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000378:metabolism*; D059808:Polyphenols / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D027542:Rosmarinus / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014461:Ultracentrifugation
chemical_list: D002457:Cell Extracts; D010936:Plant Extracts; D059808:Polyphenols; D011506:Proteins; D000432:Methanol