title: Improved viability and activity of neutrophils differentiated from HL-60 cells by co-culture with adipose tissue-derived mesenchymal stem cells.
abstract: Neutropenia is a principal complication of cancer treatment. We investigated the supportive effect of adipose tissue-derived mesenchymal stem cells (AD-MSCs) on the viability and function of neutrophils. Neutrophils were derived from HL-60 cells by dimethylformamide stimulation and cultured with or without AD-MSCs under serum-starved conditions to evaluate neutrophil survival, proliferation, and function. Serum starvation resulted in the apoptosis of neutrophils and decreased cell survival. The co-culture of neutrophils and AD-MSCs resulted in cell survival and inhibited neutrophil apoptosis under serum-starved conditions. The survival rate of neutrophils was prolonged up to 72 h, and the expression levels of interferon (IFN)-α, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor, and transforming growth factor (TGF)-β in AD-MSCs were increased after co-culture with neutrophils. AD-MSCs promoted the viability of neutrophils by inhibiting apoptosis as well as enhancing respiratory burst, which could potentially be mediated by the increased expression of IFN-α, G-CSF, and TGF-β. Thus, we conclude that the use of AD-MSCs may be a promising cell-based therapy for increasing immunity by accelerating neutrophil function.
authors.affiliation: Department of Pediatrics, Ewha Womans University, School of Medicine, Ewha Medical Research Center, Seoul, Republic of Korea.
authors.forename: Yoon Shin
authors.initials: YS
authors.lastname: Park
authors.forename: Goh-Woon
authors.initials: GW
authors.lastname: Lim
authors.forename: Kyung-Ah
authors.initials: KA
authors.lastname: Cho
authors.forename: So-Youn
authors.initials: SY
authors.lastname: Woo
authors.forename: Meeyoung
authors.initials: M
authors.lastname: Shin
authors.forename: Eun-Sun
authors.initials: ES
authors.lastname: Yoo
authors.forename: Jeong
authors.initials: J
authors.lastname: Chan Ra
authors.forename: Kyung-Ha
authors.initials: KH
authors.lastname: Ryu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D002454:Cell Differentiation; D002470:Cell Survival / Q000235:genetics; D018920:Coculture Techniques; D015870:Gene Expression; D016179:Granulocyte Colony-Stimulating Factor / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology; D018922:HL-60 Cells; D006801:Humans; D016898:Interferon-alpha / Q000276:immunology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D009503:Neutropenia / Q000628:therapy; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015291:Transforming Growth Factors / Q000276:immunology
chemical_list: D016898:Interferon-alpha; D017382:Reactive Oxygen Species; D016179:Granulocyte Colony-Stimulating Factor; D015291:Transforming Growth Factors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor