title: Use of attenuated total reflectance Fourier transform infrared spectroscopy to monitor the development of lipid aggregate structures.
abstract: Attenuated total reflectance Fourier transform infrared spectroscopy is used to monitor the adsorption of 100 nm 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles to the surfaces of Ge, electrolessly deposited Au, and a well formed self-assembled monolayer of 1-octadecanethiol. The interaction of DPPC vesicles in solution with these different surfaces yields distinctly different surface structures: intact DPPC vesicles on Ge, a supported phospholipid bilayer on an electrolessly deposited Au surface, and a phospholipid monolayer onto the hydrophobic self-assembled monolayer. IR peak position, bandwidth, and intensity are used to confirm structure formation and quantitation of the amount of lipid that desorbs during film formation.
authors.affiliation: Department of Chemistry, University of California-Davis, Davis, California 95616, USA.
authors.forename: Mateo R
authors.initials: MR
authors.lastname: Hernandez
authors.forename: Elyse N
authors.initials: EN
authors.lastname: Towns
authors.forename: Terry C
authors.initials: TC
authors.lastname: Ng
authors.forename: Brian C
authors.initials: BC
authors.lastname: Walsh
authors.forename: Richard
authors.initials: R
authors.lastname: Osibanjo
authors.forename: Atul N
authors.initials: AN
authors.lastname: Parikh
authors.forename: Donald P
authors.initials: DP
authors.lastname: Land
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000327:Adsorption; D005857:Germanium; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry; D008567:Membranes, Artificial; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D008051:Lipid Bilayers; D008563:Membrane Lipids; D008567:Membranes, Artificial; D005857:Germanium; D015060:1,2-Dipalmitoylphosphatidylcholine