title: Detection of glycoproteins in polyacrylamide gels using Pro-Q Emerald 300 dye, a fluorescent periodate Schiff-base stain.
abstract: Pro-Q Emerald 300 glycoprotein stain generates a bright-green fluorescent signal upon reacting with periodic acid-oxidized carbohydrate groups on proteins. With this dye, it is possible to detect proteins directly in the gel without the need to transfer them to a membrane. This dye is more sensitive than the standard periodic acid Schiff's base which uses acidic fuchsin dye.
authors.affiliation: Oklahoma Medical Research Foundation, Oklahoma City, OK, USA. Padmaja-Mehta@omrf.org
authors.forename: Padmaja
authors.initials: P
authors.lastname: Mehta-D'souza
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D006023:Glycoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D006834:Hydrazines / Q000737:chemistry*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D012835:Silver Nitrate / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000180:Acrylic Resins; D005456:Fluorescent Dyes; D006023:Glycoproteins; D006834:Hydrazines; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D011994:Recombinant Proteins; C016680:polyacrylamide gels; D012835:Silver Nitrate