title: Correlation of Y-chromosome multiple  segmental deletions and chromosomal anomalies in non-obstructive azoospermic males from  northeastern China.
abstract: We investigated the frequency and types of Y-chromosome  microdeletions and chromosomal anomalies in non-obstructive azoospermic and  severely oligozoospermic infertile males in northeastern China. The sample  consisted of 519 infertile males (456 azoospermic, 63 severely  oligozoospermic). PCR assays for Y-chromosome microdeletions and chromosome  analysis were performed on all patients and controls. Array-comparative genomic  hybridization was performed for three patients with chromosomal anomalies.  Fifty-nine of 519 patients (11.37%) had Y-chromosome microdeletions. Microdeletions  were found in 11.18% (51/456) of the non-obstructive azoospermic patients and  in 12.7% (8/63) of the severely oligozoospermic patients. Eleven of 51  non-obstructive azoospermic patients with Y-chromosome microdeletions had  multiple segmental deletions in the AZFb+c regions; four of these patients had  chromosomal anomalies. Our sample from northeastern China had a higher  frequency of microdeletions among severely oligozoospermic than among  non-obstructive azoospermic males.
authors.affiliation: Center for Reproductive Medicine, First Bethune Hospital, Jilin University, Changchun, China.
authors.forename: R-L
authors.initials: RL
authors.lastname: Dai
authors.forename: R-X
authors.initials: RX
authors.lastname: Wang
authors.forename: J-L
authors.initials: JL
authors.lastname: Jin
authors.forename: G-N
authors.initials: GN
authors.lastname: Niu
authors.forename: J-Y
authors.initials: JY
authors.lastname: Lee
authors.forename: S-B
authors.initials: SB
authors.lastname: Li
authors.forename: R-Z
authors.initials: RZ
authors.lastname: Liu
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics*; D002681:China; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D006801:Humans; D007248:Infertility, Male; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D016324:Sequence Tagged Sites; D012729:Sex Chromosome Aberrations; D058533:Sex Chromosome Disorders of Sex Development / Q000235:genetics; D055815:Young Adult