title: Possible novel roles of poly(rC)-binding protein 1 in SH-SY5Y neurocytes: an analysis using a dynamic Bayesian network.
abstract: OBJECTIVE
  Poly(rC)-binding protein 1 (PCBP1) belongs to the heterogeneous nuclear ribonucleoprotein family and participates in transcriptional and translational regulation. Previous work has identified transcripts targeted by both knockdown and overexpression of PCBP1 in SH-SY5Y neuroblastoma cells using a microarray or ProteomeLab protein fractionation 2-dimensions (PF-2D) and quadrupole time-of-flight mass spectrometer. The present study aimed to further determine whether these altered transcripts from major pathways (such as Wnt signaling, TGF-β signaling, cell cycling, and apoptosis) and two other genes, H2AFX and H2BFS (screened by PF-2D), have spatial relationships.
  
  
  METHODS
  The genes were studied by qRT-PCR, and dynamic Bayesian network analysis was used to rebuild the coordination network of these transcripts.
  
  
  RESULTS
  PCBP1 controlled the expression or activity of the seven transcripts. Moreover, PCBP1 indirectly regulated MAP2K2, FOS, FST, TP53 and WNT7B through H2AFX or regulated these genes through SAT. In contrast, TP53 and WNT7B are regulated by other genes.
  
  
  CONCLUSIONS
  The seven transcripts and PCBP1 are closely associated in a spatial interaction network.
authors.affiliation: Department of Physiology, Key Laboratory for Neurodegenerative Disorders of Ministry of Education, Capital Medical University, Beijing 100069, China.
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Huo
authors.forename: Jian-Tao
authors.initials: JT
authors.lastname: Liang
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Zou
authors.forename: Lan-Ying
authors.initials: LY
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Wang
mesh_terms: D001499:Bayes Theorem; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins; D053263:Gene Regulatory Networks; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism*; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D016601:RNA-Binding Proteins; D015398:Signal Transduction / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C094604:PCBP1 protein, human; D016601:RNA-Binding Proteins