title: [Effect of induced-mineralization on the expression of SOST in human periodontal ligament cells].
abstract: OBJECTIVE
  To investigate the effect of induced- mineralization on the expression of SOST gene in human periodontal ligament cells, and explore the effect of SOST on periodontal tissues.
  
  
  METHODS
  Human periodontal ligament cells were cultured by tissue explant in vitro, the cells were continuously induced by mineralization solution for 7,14 and 21days.The mineralized ability, SOST and related osteogenic genes(ALP, OCN)expression were detected. The data was analyzed with SPSS 17.0 software package.
  
  
  RESULTS
  Under the condition of mineralization solution in culture medium,human periodontal ligament cells had stronger ALP activity and Alizarin red staining,SOST and related osteogenic genes(ALP,OCN) expression were significantly upregulated in a time-dependent manner(P<0.05).
  
  
  CONCLUSIONS
  After continuous induced-mineralization culture, human periodontal ligament cells can express SOST mRNA and related osteogenic genes in a time-dependent manner, which suggests that SOST may play a role in the regeneration of periodontal tissues.
authors.affiliation: Department of Prosthodontics, Shanghai Jiao Tong University School of Medicine, Shanghai, China. steflinting@sina.cn
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Lin
authors.forename: Er-yi
authors.initials: EY
authors.lastname: Lu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D019485:Bone Morphogenetic Proteins; D002454:Cell Differentiation; D005819:Genetic Markers; D006801:Humans; D010012:Osteogenesis; D010513:Periodontal Ligament; D010519:Periodontium; D012038:Regeneration
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D019485:Bone Morphogenetic Proteins; D005819:Genetic Markers; C422903:SOST protein, human