title: Simultaneous determination of caffeic acid phenethyl ester and its metabolite caffeic acid in dog plasma using liquid chromatography tandem mass spectrometry.
abstract: A simple, reliable and sensitive method for the simultaneous determination of caffeic acid phenethyl ester (CAPE) and its metabolite caffeic acid (CA) in dog plasma was developed using liquid chromatography tandem mass spectrometry (LC-MS/MS). The sample pretreatment generally involved protein precipitation treatment (PPT) and direct dilution. CAPE and CA were separated with a C18 reversed-phase column. Electrospray ionization (ESI) interface operated in negative mode was chosen for ionization. Multiple reaction monitoring (MRM) mode was selected for data acquisition. The quantification range was 10.0-10,000.0 ng mL(-1). The intra- and inter-batch accuracies were within 92.5-107.0% with relative standard deviation (RSD, %) no more than 10.5%. CAPE and CA were proved to be stable in stabilizer-treated dog blood and PPT-treated plasma during the sampling and pretreatment period. The applicability has been evaluated with real samples from treated dogs.
authors.affiliation: State Key Laboratory of Organic Geochemistry, Guangzhou Geochemistry Institute of Chinese Academy of Sciences, Guangzhou 510640, China. tcmcasgig@gmail.com
authors.forename: Caiming
authors.initials: C
authors.lastname: Tang
authors.forename: O Samuel
authors.initials: OS
authors.lastname: Sojinu
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D011232:Chemical Precipitation; D002853:Chromatography, Liquid; D004285:Dogs; D004355:Drug Stability; D010626:Phenylethyl Alcohol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D002109:Caffeic Acids; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol; C040048:caffeic acid