title: Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family.
abstract: Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.
authors.affiliation: Biomolecular Function Research Branch, Division of Convergence Technology, Research Institute, National Cancer Center, Goyang, Gyeonggi, Republic of Korea.
authors.forename: Byeong-Gu
authors.initials: BG
authors.lastname: Han
authors.forename: Kyung-Chae
authors.initials: KC
authors.lastname: Jeong
authors.forename: Jea-Won
authors.initials: JW
authors.lastname: Cho
authors.forename: Byung-Cheon
authors.initials: BC
authors.lastname: Jeong
authors.forename: Hyun Kyu
authors.initials: HK
authors.lastname: Song
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Dong Hyuk
authors.initials: DH
authors.lastname: Shin
authors.forename: Sangho
authors.initials: S
authors.lastname: Lee
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D020027:Pyrococcus furiosus
chemical_list: D019843:Archaeal Proteins; D004247:DNA