title: Two unique recombinant forms identified in incident HIV type 1 infections in Thai blood donors.
abstract: HIV-1 genetic diversity of recently seroconverting (<12 months) Thai repeated blood donors attending the National Blood Centre, Thai Red Cross Society (NBC, TRCS) from September 2007 until March 2008 was assessed. Ten HIV-1 recent seroconvertors (10/239,134 donations) were identified during the study period. The estimated median time to seroconversion was 67.3 days (range: 45.5-102.0 days), and viral load ranged from 307 to 341,805 copies HIV-1 RNA/ml. MHAbce, a real-time-based PCR genotyping assay, identified six CRF01_AE, two CRF01_AE/B recombinants, one subtype B, and one CRF01_AE/B dual infection. Nine samples were further characterized by full genome sequencing, identifying CRF01_AE (N=6), unique CRF01_AE/B recombinants (N=2), and subtype B (N=1). One recombinant contained 13 breakpoints located in gag, pol, vif, vpr, env, and nef while the other recombinant contained 10 breakpoints located in pol, vif, env, and nef. This study found two unique CRF01B recombinants circulating in 10 recent HIV-1-positive subjects from a blood donor population in Thailand.
authors.affiliation: Department of Retrovirology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. wiriyar@afrims.org
authors.forename: Wiriya
authors.initials: W
authors.lastname: Rutvisuttinunt
authors.forename: Sunee
authors.initials: S
authors.lastname: Sirivichayakul
authors.forename: Sineenart
authors.initials: S
authors.lastname: Oota
authors.forename: Vatcharain
authors.initials: V
authors.lastname: Assawadarachai
authors.forename: Kultida
authors.initials: K
authors.lastname: Poltavee
authors.forename: Hathairat
authors.initials: H
authors.lastname: Savadsuk
authors.forename: Supanit
authors.initials: S
authors.lastname: Pattanachaiwit
authors.forename: Suwittra
authors.initials: S
authors.lastname: Chaemchuen
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Arroyo
authors.forename: Robert M
authors.initials: RM
authors.lastname: Paris
authors.forename: Nelson L
authors.initials: NL
authors.lastname: Michael
authors.forename: Jerome H
authors.initials: JH
authors.lastname: Kim
authors.forename: Kiat
authors.initials: K
authors.lastname: Ruxrungtham
authors.forename: Mark
authors.initials: M
authors.lastname: de Souza
authors.forename: Praphan
authors.initials: P
authors.lastname: Phanuphak
authors.forename: Sodsai
authors.initials: S
authors.lastname: Tovanabutra
mesh_terms: D000328:Adult; D001782:Blood Donors; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013785:Thailand; D055815:Young Adult