title: Surf4 modulates STIM1-dependent calcium entry.
abstract: Store-operated Ca(2+) entry (SOCE) is crucial for various physiological responses in immune cells. Although it is known that STIM1 relocates into discrete puncta juxtaposed to the plasma membrane to initiate SOCE, the machinery modulating the function of STIM1 remains unclear. We explored to find its modulators using affinity purification for STIM1-binding proteins and identified surfeit locus protein 4 (Surf4). Surf4 associated with STIM1 in the endoplasmic reticulum. Deletion of Surf4 in DT40 B cells resulted in marked increase of SOCE and facilitation of STIM1 clustering upon store-depletion. These findings suggest the modulatory function of Surf4 for STIM1-mediated SOCE.
authors.affiliation: Laboratory for Lymphocyte Differentiation, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Fujii
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shiota
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ohkawa
authors.forename: Akemi
authors.initials: A
authors.lastname: Baba
authors.forename: Hideki
authors.initials: H
authors.lastname: Wanibuchi
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kinashi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kurosaki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Baba
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D017384:Sequence Deletion; D000071737:Stromal Interaction Molecule 1
chemical_list: D015220:Calcium Channels; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C100757:Stim1 protein, mouse; D000071737:Stromal Interaction Molecule 1; C574718:Surf4 protein, mouse; D002118:Calcium