title: Human internal mammary artery (IMA) transplantation and stenting: a human model to study the development of in-stent restenosis.
abstract: Preclinical in vivo research models to investigate pathobiological and pathophysiological processes in the development of intimal hyperplasia after vessel stenting are crucial for translational approaches (1,2). The commonly used animal models include mice, rats, rabbits, and pigs (3-5). However, the translation of these models into clinical settings remains difficult, since those biological processes are already studied in animal vessels but never performed before in human research models (6,7). In this video we demonstrate a new humanized model to overcome this translational gap. The shown procedure is reproducible, easy, and fast to perform and is suitable to study the development of intimal hyperplasia and the applicability of diverse stents. This video shows how to perform the stent technique in human vessels followed by transplantation into immunodeficient rats, and identifies the origin of proliferating cells as human.
authors.affiliation: University Heart Center Hamburg, TSI-Lab, Germany.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Hua
authors.forename: Tobias
authors.initials: T
authors.lastname: Deuse
authors.forename: Evangelos D
authors.initials: ED
authors.lastname: Michelakis
authors.forename: Alois
authors.initials: A
authors.lastname: Haromy
authors.forename: Phil S
authors.initials: PS
authors.lastname: Tsao
authors.forename: Lars
authors.initials: L
authors.lastname: Maegdefessel
authors.forename: Reinhold G
authors.initials: RG
authors.lastname: Erben
authors.forename: Claudia
authors.initials: C
authors.lastname: Bergow
authors.forename: Boris B
authors.initials: BB
authors.lastname: Behnisch
authors.forename: Hermann
authors.initials: H
authors.lastname: Reichenspurner
authors.forename: Robert C
authors.initials: RC
authors.lastname: Robbins
authors.forename: Sonja
authors.initials: S
authors.lastname: Schrepfer
mesh_terms: D000818:Animals; D048708:Cell Growth Processes / Q000502:physiology; D006083:Graft Occlusion, Vascular / Q000209:etiology* / Q000473:pathology; D006801:Humans; D008323:Mammary Arteries / Q000166:cytology / Q000637:transplantation*; D051381:Rats; D011923:Rats, Nude; D015607:Stents; D014183:Transplantation, Heterologous; D058017:Vascular Grafting / Q000379:methods*