title: Physicochemical characterization and cytotoxic activity evaluation of hydroxymethylferrocene:β-cyclodextrin inclusion complex.
abstract: An inclusion complex of hydroxymethylferrocene (FeMeOH) with β-cyclodextrin (β-CD) was prepared in the solid state by different techniques such as physical mixture, coprecipitation, kneading and freeze-drying. The formation of the inclusion complex was confirmed by X-ray Powder Diffractometry and Fourier Transform-Infrared spectroscopy. In aqueous solution, the 1:1 stoichiometry was established by a Job plot. The inclusion complex formation was also investigated by NMR and the stability constant (Kb) of the complex was determined to be 478 M⁻¹, which is in agreement with that obtained with UV-Vis tritation (Kb = 541.3 M⁻¹). The phase solubility study showed a diagram classified as Bs type and that the solubility of FeMeOH was slightly increased in the presence of β-CD. Furthermore, utilizing phase solubility diagram data, the Kb was estimated to be equal to 528.0 M⁻¹. The cytotoxic activity of FeMeOH and its complexation product with β-CD was determined using the MTT-assay on MDA-MB-231 cell line, showing that the inclusion complex has a higher capability of inhibiting cell growth compared to that of pure FeMeOH.
authors.affiliation: Department of Environmental Science, Second University of Naples, via A.Vivaldi 43, 81100 Caserta, Italy. rosa.iacovino@unina2.it
authors.forename: Rosa
authors.initials: R
authors.lastname: Iacovino
authors.forename: Jolanda Valentina
authors.initials: JV
authors.lastname: Caso
authors.forename: Filomena
authors.initials: F
authors.lastname: Rapuano
authors.forename: Agostino
authors.initials: A
authors.lastname: Russo
authors.forename: Marina
authors.initials: M
authors.lastname: Isidori
authors.forename: Margherita
authors.initials: M
authors.lastname: Lavorgna
authors.forename: Gaetano
authors.initials: G
authors.lastname: Malgieri
authors.forename: Carla
authors.initials: C
authors.lastname: Isernia
mesh_terms: D045744:Cell Line, Tumor; D005296:Ferrous Compounds / Q000737:chemistry* / Q000633:toxicity*; D006801:Humans; D012995:Solubility; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D005296:Ferrous Compounds; D047392:beta-Cyclodextrins