title: Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in polyvinylpyrrolidone-based sperm-immobilization medium.
abstract: OBJECTIVE
  To elucidate the effect of sperm immobilization media that are and are not based on polyvinylpyrrolidone (PVP) on the DNA integrity of fresh and frozen-thawed spermatozoa during standard intracytoplasmic sperm injection (ICSI) conditions.
  
  
  METHODS
  Experimental prospective study.
  
  
  METHODS
  Embryology research laboratory.
  
  
  METHODS
  Forty-six ejaculates from normozoospermic and oligozoospermic men.
  
  
  METHODS
  Assessment of sperm DNA fragmentation by single-cell gel electrophoresis assay.
  
  
  METHODS
  DNA integrity of fresh and frozen-thawed spermatozoa from normozoospermic and oligozoospermic ejaculates exposed to PVP-based and non-PVP-based media.
  
  
  RESULTS
  Exposure of fresh and frozen thawed spermatozoa from normozoospermic and oligozoospermic ejaculates to PVP-based medium in an ICSI dish for 30 minutes statistically significantly increased the DNA fragmentation. In contrast, the extent of DNA fragmentation in non-PVP-based medium did not statistically significantly differ from control.
  
  
  CONCLUSIONS
  A PVP-based medium can induce a statistically significant amount of sperm DNA fragmentation in an ICSI dish, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.
authors.affiliation: Clinical Embryology, Kasturba Medical College, Manipal University, Manipal, India.
authors.forename: Sujit Raj
authors.initials: SR
authors.lastname: Salian
authors.forename: Guruprasad
authors.initials: G
authors.lastname: Kalthur
authors.forename: Shubhashree
authors.initials: S
authors.lastname: Uppangala
authors.forename: Pratap
authors.initials: P
authors.lastname: Kumar
authors.forename: Satish Kumar
authors.initials: SK
authors.lastname: Adiga
mesh_terms: D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D053938:DNA Fragmentation / Q000187:drug effects*; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000235:genetics / Q000628:therapy*; D011205:Povidone / Q000737:chemistry / Q000494:pharmacology*; D011446:Prospective Studies; D027724:Reproductive Techniques, Assisted; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
chemical_list: D003470:Culture Media; D011205:Povidone