title: Antimicrobial activity of pediocin PA-1 against Oenococcus oeni and other wine bacteria.
abstract: Pediocin PA-1 is an antimicrobial peptide produced by lactic acid bacteria (LAB) that has been sufficiently well characterised to be used in food industry as a biopreservative. Sulphur dioxide is the traditional antimicrobial agent used during the winemaking process to control bacterial growth and wine spoilage. In this study, we describe the effect of pediocin PA-1 alone and in combination with sulphur dioxide and ethanol on the growth of a collection of 53 oenological LAB, 18 acetic acid bacteria and 16 yeast strains; in addition, production of pediocin PA-1 by Pediococcus acidilactici J347-29 in presence of ethanol and grape must is also reported. Inhibitory concentrations (IC) and minimal bactericide concentrations of pediocin PA-1 were determined against LAB, and revealed a bacteriostatic effect. Oenococcus oeni resulted more sensitive to pediocin PA-1 (IC(50) = 19 ng/ml) than the other LAB species (IC(50) = 312 ng/ml). Cooperative inhibitory effects of pediocin PA-1 and either sulphur dioxide or ethanol were observed on LAB growth. Moreover, the pediocin PA-1 producing P. acidilactici strain J347-29 was able to grow and produce the bacteriocin in presence of ethanol (up to 4% ethanol in the fermentation broth) and grape must (up to 80%), which indicated that pediocin PA-1 can be considered as a potential biopreservative in winemaking.
authors.affiliation: University of La Rioja, Instituto de Ciencias de la Vid y del Vino (CSIC, Universidad de La Rioja, Gobierno de La Rioja), Departamento de Agricultura y Alimentación, Av. Madre de Dios 51, 26006 Logroño, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Díez
authors.forename: Beatriz
authors.initials: B
authors.lastname: Rojo-Bezares
authors.forename: Myriam
authors.initials: M
authors.lastname: Zarazaga
authors.forename: Juan M
authors.initials: JM
authors.lastname: Rodríguez
authors.forename: Carmen
authors.initials: C
authors.lastname: Torres
authors.forename: Fernanda
authors.initials: F
authors.lastname: Ruiz-Larrea
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D005285:Fermentation; D056585:Oenococcus / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D000070017:Pediocins; D010376:Pediococcus / Q000737:chemistry / Q000378:metabolism; D014920:Wine / Q000032:analysis / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D000070017:Pediocins; C074288:pediocin PA-1