title: Similar incidences of TP53 deletions in extramedullary organ infiltrations, soft tissue and osteolyses of patients with multiple myeloma.
abstract: BACKGROUND
  Extramedullary (EM) organ impairment in patients with multiple myeloma (MM) is a rare event, occurring mostly during disease relapse after high-dose chemotherapy with autologous or allogeneic stem cell transplantation. This manifestation is commonly associated with an unfavourable outcome. Previous studies suggested a correlation between the clinical course of patients with MM and EM and the cytogenetic findings, e.g. deletion of TP53 on 17p13.
  
  
  METHODS
  We investigated patients with these rare plasma cell organ infiltrations (n=17) as well as bone lesions or soft tissue lesions, known to be a common clinical feature of MM (n=14), using a newly established method of fluorescence in situ hybridization in combination with cytoplasmic immunoglobulin staining (cIg-FISH) on paraffin-embedded sections and a specific probe for TP53 on 17p13.
  
  
  CONCLUSIONS
  The incidence of del(17)(p13) was similar in both groups but overall it was higher when compared to published data obtained from bone marrow samples and material originating from osteolyses. Further investigations on a larger patient cohort are needed in order to confirm these findings.
authors.affiliation: Department of Oncology, Hematology and Bone Marrow Transplantation with Section Pneumology, University Medical Center Hamburg-Eppendorf, Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Billecke
authors.forename: Eva Maria Murga
authors.initials: EM
authors.lastname: Penas
authors.forename: Annette M
authors.initials: AM
authors.lastname: May
authors.forename: Monika
authors.initials: M
authors.lastname: Engelhardt
authors.forename: Arnon
authors.initials: A
authors.lastname: Nagler
authors.forename: Merav
authors.initials: M
authors.lastname: Leiba
authors.forename: Ginette
authors.initials: G
authors.lastname: Schiby
authors.forename: Nicolaus
authors.initials: N
authors.lastname: Kröger
authors.forename: Jozef
authors.initials: J
authors.lastname: Zustin
authors.forename: Andreas
authors.initials: A
authors.lastname: Marx
authors.forename: Jakob
authors.initials: J
authors.lastname: Matschke
authors.forename: Markus
authors.initials: M
authors.lastname: Tiemann
authors.forename: Eray
authors.initials: E
authors.lastname: Goekkurt
authors.forename: Carsten
authors.initials: C
authors.lastname: Bokemeyer
authors.forename: Georgia
authors.initials: G
authors.lastname: Schilling
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17; D005260:Female; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000150:complications / Q000235:genetics* / Q000473:pathology; D010014:Osteolysis / Q000209:etiology*; D012983:Soft Tissue Neoplasms / Q000235:genetics* / Q000473:pathology