title: [Proteomics-based identification of proapoptotic caspase adapter protein as a novel serum marker of non-small cell lung cancer].
abstract: OBJECTIVE
  Early diagnosis and treatment of lung cancer contribute to a patient's prolonged survival. Proteomics and tissue culture were used to screen potential serum markers of lung cancer.
  
  
  METHODS
  The differential proteomic technique and matrix-assisted laser desorption/ionization-time of flight mass spectrometry were used to identify overexpressed proteins from cultured tumor tissues in a conditioned medium. Paired lung tissues in a conditioned medium were used as the control group. Overexpressed proapoptotic caspase adaptor protein (PACAP) was identified using Western blot and enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The expression of nineteen protein spots exhibited a twofold increase compared with that in the control group. Fourteen of which were identified, including the distinctly overexpressed PACAP. Western blot analysis revealed a high level of PACAP in the lung adenocarcinoma in both tissue culture media and patients' serum. Overexpressed PACAP was also detected in the serum of non-small cell lung cancer patients via ELISA compared with that in the serum of small cell lung cancer patients, benign lung tumor patients, and healthy individuals (P<0.05).
  
  
  CONCLUSIONS
  PACAP is a potential serum marker of non-small cell lung cancer.
authors.affiliation: Department of Cardiothoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Shengxi
authors.initials: S
authors.lastname: Chen
authors.forename: Lingjin
authors.initials: L
authors.lastname: Huang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000097:blood* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000097:blood* / Q000378:metabolism; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D017077:Culture Media, Conditioned / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D046509:Tissue Culture Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D017077:Culture Media, Conditioned; C434482:MZB1 protein, human