title: Urinary metabolite variation is associated with pathological progression of the post-hepatitis B cirrhosis patients.
abstract: Cirrhosis is a common and terminal outcome of many chronic liver conditions. A urinary metabonomic study using gas chromatography-mass spectrometry (GC-MS) and ultra performance liquid chromatography time-of-flight mass spectrometry (UPLC-TOFMS) was carried out to elucidate the pathophysiological basis of posthepatitis B cirrhosis in 63 posthepatitis B cirrhosis patients and 31 health controls. Urinary metabolic profile and corresponding differential metabolites associated with Child-Pugh (CP) grading of liver function were characterized, in addition to the blood routine, liver, and renal function tests. Multivariate statistical tools including principal component analysis (PCA) and orthogonal partial least-squares-discriminant analysis (OPLS-DA) were employed in the metabolite analysis along with a univariate statistical method, Wilcoxon-Mann-Whitney test. The alterations of differential metabolites contributing to the intergroup variation between healthy controls and cirrhotic patients, and among cirrhosis of CP grade A, B and C were also investigated. Six metabolites, α-hydroxyhippurate, tyrosine-betaxanthin, 3-hydroxyisovalerate, canavaninosuccinate, estrone, and glycoursodeoxycholate, were significantly altered among cirrhotic patients with CP A, B, and C, reflecting abnormal metabolism of amino acid, bile acids, hormones, and intestinal microbial metabolism. The results show that dynamic alteration of urinary metabolome, characterized by the changes of a panel of the differential metabolite markers, is indicative of an exacerbated liver function, highlighting their diagnostic and prognostic potential for the liver cirrhosis development.
authors.affiliation: E-institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Xie
authors.forename: Mingmei
authors.initials: M
authors.lastname: Zhou
authors.forename: Huan
authors.initials: H
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.forename: Guangli
authors.initials: G
authors.lastname: Du
authors.forename: Guoan
authors.initials: G
authors.lastname: Luo
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D018450:Disease Progression; D006515:Hepatitis B virus; D019694:Hepatitis B, Chronic / Q000473:pathology / Q000652:urine*; D006801:Humans; D008103:Liver Cirrhosis / Q000473:pathology / Q000652:urine* / Q000821:virology; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D025341:Principal Component Analysis; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers