title: Biomimetic smart nanocomposite: in vitro biological evaluation of zein electrospun fluorescent nanofiber encapsulated CdS quantum dots.
abstract: New hybrid quantum dot (QD)/nanofibers have potential applications in a variety of fields. A novel fluorescent nanocomposite nanofiber material, consisting of CdS and zein has been fabricated through the electrospinning process. A detailed optimization was carried out to fabricate continuous and uniform nanofibers without beads or droplets. The synthesized hybrid nanofibers were characterized by various state-of-the-art techniques such as scanning electron microscopy, transmission electron microscopy (TEM), TEM-energy dispersive spectrometry, atomic force microscopy and confocal fluorescence micrography. The optimization process was carried out to fabricate fibers ranging from 200 to 450 nm in diameter. The electrical conductivity of the zein-CdS hybrid nanofiber substrates was tested. The potential use of the electrospun CdS-encapsulated nanofibrous scaffold as substrates for cell/tissue culture was evaluated with two different cell types, i.e. mesenchymal stem cells and fibroblasts. The results showed that the electrospun fibrous scaffolds could support the attachment and the proliferation of cells. In addition, the cells cultured on the fibrous scaffolds exhibited normal cell shapes and integrated well with surrounding fibers. The obtained results confirmed the potential for the use of the electrospun QD-encapsulated fluorescent nanofiber mats as scaffolds for tissue engineering.
authors.affiliation: Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan.
authors.forename: Brahatheeswaran
authors.initials: B
authors.lastname: Dhandayuthapani
authors.forename: Aby Cheruvathoor
authors.initials: AC
authors.lastname: Poulose
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nagaoka
authors.forename: Takashi
authors.initials: T
authors.lastname: Hasumura
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Toru
authors.initials: T
authors.lastname: Maekawa
authors.forename: D Sakthi
authors.initials: DS
authors.lastname: Kumar
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D019187:Cadmium Compounds / Q000737:chemistry*; D002448:Cell Adhesion; D048430:Cell Shape; D002470:Cell Survival / Q000187:drug effects; D004553:Electric Conductivity; D055664:Electrochemical Techniques; D005347:Fibroblasts; D005456:Fluorescent Dyes; D059630:Mesenchymal Stem Cells; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D045663:Quantum Dots; D013440:Sulfides / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry; D015028:Zein / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D013440:Sulfides; C034939:cadmium sulfide; D015028:Zein