title: Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR.
abstract: Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance.
authors.affiliation: Instituto de Virología Dr. J. M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina. pisanomb@yahoo.com
authors.forename: María Belén
authors.initials: MB
authors.lastname: Pisano
authors.forename: María Paz Sánchez
authors.initials: MP
authors.lastname: Seco
authors.forename: Viviana Elizabeth
authors.initials: VE
authors.lastname: Ré
authors.forename: Adrián A
authors.initials: AA
authors.lastname: Farías
authors.forename: Marta Silvia
authors.initials: MS
authors.lastname: Contigiani
authors.forename: Antonio
authors.initials: A
authors.lastname: Tenorio
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004666:Encephalitis Virus, Venezuelan Equine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012680:Sensitivity and Specificity; D013997:Time Factors; D014773:Virology / Q000379:methods* / Q000592:standards
chemical_list: D017931:DNA Primers