title: Protective effect of Lysimachia christinae against acute alcohol-induced liver injury in mice.
abstract: Lysimachia christinae Hance (Primulaceae) is a medicinal plant. The present study was undertaken to investigate protection of L. christinae against acute alcohol-induced liver injury in mice, the related mechanism of oxidative stress and its hepatoprotective chemical compound for the first time. Mice were orally administered alcohol at 6 g/kg 2 h after a 75% ethanol extract of L. christinae (ET) (100, 200, 400 mg/kg), quercetin (2, 4, 8 mg/kg) isolated from L. christinae, or bifendate (150 mg/kg) for seven consecutive days by intragastric administration (i.g.) except the normal group. Serum and liver tissue samples were collected 6 h after alcohol administration and the amount of quercetin in ET was analyzed by high-performance liquid chromatography (HPLC) with a diode array detector (DAD). The results showed that alcohol-induced elevated serum alanine transferase (ALT) and aspartate transaminase (AST) activities were significantly reduced by ET (200, 400 mg/kg), quercetin (4, 8 mg/kg) and bifendate (150 mg/kg), respectively. Further analysis demonstrated that lipid peroxidation (LPO) levels significantly decreased, while glutathione amounts, glutathione-s transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) activities all increased in livers of ET-, quercetin-, and bifendate-treated mice. Besides, amount of quercetin in ET was 1.03%. Taken together, our results indicate that L. christinae can protect against acute alcohol-induced liver injury in mice, the potential mechanism can be related to inhibiting liver oxidative stress injury, and its main hepatoprotective compound is quercetin, for the first time.
authors.affiliation: School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, China. mjw98_2010@163.com
authors.forename: Junming
authors.initials: J
authors.lastname: Wang
authors.forename: Yueyue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yasu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Cui
authors.forename: Ju
authors.initials: J
authors.lastname: Liu
authors.forename: Binfeng
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000627:therapeutic use; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D000431:Ethanol / Q000009:adverse effects*; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D029623:Primulaceae / Q000737:chemistry*; D011794:Quercetin / Q000627:therapeutic use
chemical_list: D001713:Biphenyl Compounds; D010936:Plant Extracts; C465548:bifendate; D000431:Ethanol; D011794:Quercetin