title: An improved strategy based on  RAPD markers efficiently identified 95 peach cultivars.
abstract: DNA  markers have useful applications in cultivar identification. A novel analysis  approach called cultivar identification diagram (CID) was developed using DNA  markers in the separation of plant individuals. This new strategy is less  time- and cost-consuming, has reliable results, and was constructed for  fingerprinting. Ten 11-mer primers were used to amplify the genotypes; all 95  peach genotypes (from the National Peach Germplasm Repository, in Nanjing,  China) were distinguished by a combination of 54 primers. The utilization of  the CID among these 95 peach cultivars was also verified by the identification  of three randomly chosen groups of cultivars. This identification showed some  advantages including the use of fewer primers and easy separation of all  cultivars by the corresponding primers marked in the right position on the CID.  This peach CID could provide the information to separate any peach cultivars of  these 95, which may be of help to the peach industry in China and for the  utilization of DNA markers to identify other plant species.
authors.affiliation: Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing, PR China. mly1008@yahoo.com.cn
authors.forename: M L
authors.initials: ML
authors.lastname: Yu
authors.forename: W Y
authors.initials: WY
authors.lastname: Wang
authors.forename: R J
authors.initials: RJ
authors.lastname: Ma
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Shen
authors.forename: J G
authors.initials: JG
authors.lastname: Fang
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers; D027861:Prunus / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers