title: Adipose-derived stem cells on the healing of ischemic colitis: a therapeutic effect by angiogenesis.
abstract: BACKGROUND
  There has been no specific treatment for ischemic colitis. We verified the effects of adipose-derived stem cells (ASCs) on ischemia-induced colitis in a rat model.
  
  
  METHODS
  Forty male Sprague-Dawley rats (10 weeks old; weight, 350 ± 20 g) were divided into two groups: a control group (only fibrinogen and thrombin injected, n = 20) and an ASC group (local implantation of ASCs mixed with thrombin and fibrinogen, n = 20). An ischemic colitis model was established by modifying Nagahata's methods with double-blind randomization. ASCs (1 × 10(6) cells) were implanted intramurally into the ischemic area using a fibrin glue mixture. The severity of adhesion, degree of ileus, the number and size of the ulcers, Wallace macroscopic and microscopic scores, and microvascular density were measured.
  
  
  RESULTS
  The degree of ileus was significantly lower, and significantly fewer and smaller ulcerations were found in the ASC group than those in the control group. Wallace macroscopic and microscopic scores were lower in the ASC group than in the control group (1.90 ± 1.22 versus 3.25 ± 1.83, p < 0.01 and 1.55 ± 1.88 versus 2.84 ± 1.89, p < 0.05, respectively). Microvascular density was higher in the ASC group than in the control (54.45 ± 19.45 versus 26.54 ± 13.14, p < 0.01, respectively).
  
  
  CONCLUSIONS
  Local implantation of ASCs into an ischemic-injured colonic wall reduced the grade of ischemic injury and enhanced tissue healing by promoting angiogenesis.
authors.affiliation: Department of Surgery, Inje University College of Medicine, Busan Paik Hospital, 633-165 Gaegum-dong, Jin-gu, Busan 614-735, South Korea.
authors.forename: Hyun Ho
authors.initials: HH
authors.lastname: Joo
authors.forename: Hye Jung
authors.initials: HJ
authors.lastname: Jo
authors.forename: Tae Doo
authors.initials: TD
authors.lastname: Jung
authors.forename: Min Sung
authors.initials: MS
authors.lastname: Ahn
authors.forename: Ki Beom
authors.initials: KB
authors.lastname: Bae
authors.forename: Kwan Hee
authors.initials: KH
authors.lastname: Hong
authors.forename: Jeong
authors.initials: J
authors.lastname: Kim
authors.forename: Jong Tae
authors.initials: JT
authors.lastname: Kim
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Young Il
authors.initials: YI
authors.lastname: Yang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D017091:Colitis, Ischemic / Q000150:complications / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D006801:Humans; D008297:Male; D055806:Microvessels / Q000473:pathology; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing
chemical_list: D042461:Vascular Endothelial Growth Factor A