title: Morphological traits and RAPD  markers for characterization and identification of minor Spanish olive  cultivars from the Extremadura region.
abstract: A total  of 91 wild olive accessions and 31 olive cultivars growing in the Extremadura  region of central-western Spain were analyzed using morphological traits and RAPD  markers. We focused on three main and 16 minor Spanish olive cultivars that are  recognized as native or local to the Extremadura region. The five arbitrary  10-mer primers tested on the olive cultivars gave 67 polymorphic bands,  representing 91% of the total amplification products. The number of bands per  primer ranged from 9 to 18, whereas the number of polymorphic bands ranged from  8 to 17. All the cultivars could be identified by a combination of three  primers (OPF-6, OPA-8, and OPK-16); four cultivar-specific markers were  detected. The minor local "Jariego" and "Tempranillo" cultivars showed the most  distal similarities. The resulting dendrogram, using the unweighted pair-group  method with arithmetic mean clustering algorithm, depicted the pattern of  relationships between the local Extremadura cultivars and the cultivars from  geographically connected regions. This analysis showed a correlation between  most of the minor local cultivars and the geographical origin; there was no  apparent clustering according to morphological traits or fruit use of olive  cultivars when these parameters were used as analysis criteria.
authors.affiliation: Plant Physiology, Faculty of Science, University of Extremadura, Badajoz, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Parra-Lobato
authors.forename: F J
authors.initials: FJ
authors.lastname: Delgado-Martinez
authors.forename: M C
authors.initials: MC
authors.lastname: Gomez-Jimenez
mesh_terms: D060146:Ecotype; D005819:Genetic Markers; D005838:Genotype; D031658:Olea / Q000033:anatomy & histology* / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019655:Quantitative Trait, Heritable; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D013030:Spain
chemical_list: D005819:Genetic Markers