title: miRNA expression profiling for identification of potential breast cancer biomarkers.
abstract: To identify micro RNA (miRNA) biomarker candidates for early detection of breast cancer and detection of minimal residual breast cancer, we performed miRNA expression profiling in pooled RNA samples from breast tumors, and from bone marrow mononuclear cells, peripheral blood mononuclear cells and plasma from healthy controls. We found substantially higher levels of five miRNAs in the breast tumors compared to the normal samples. However, validation of these miRNA levels, and seven other candidates selected from the literature, in individual samples from healthy controls and patients with non-metastatic breast cancer did not suggest further examination of their biomarker potential.
authors.affiliation: Department of Haematology and Oncology, Stavanger University Hospital, Stavanger, Norway. ktje@sus.no
authors.forename: Kjersti
authors.initials: K
authors.lastname: Tjensvoll
authors.forename: Kine N
authors.initials: KN
authors.lastname: Svendsen
authors.forename: James M
authors.initials: JM
authors.lastname: Reuben
authors.forename: Satu
authors.initials: S
authors.lastname: Oltedal
authors.forename: Bjørnar
authors.initials: B
authors.lastname: Gilje
authors.forename: Rune
authors.initials: R
authors.lastname: Smaaland
authors.forename: Oddmund
authors.initials: O
authors.lastname: Nordgård
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs