title: Construction of RNA-Seq libraries from large and microscopic tissues for the Illumina sequencing platform.
abstract: Second-generation DNA sequencing platforms have emerged as powerful tools in biological research. Their high sequence output at lower cost and minimal input DNA requirement render them suitable for broad applications ranging from gene expression studies to personalized clinical diagnostics. Here, we describe the preparation of cDNA libraries, from both whole aphid insects and their microscopic salivary gland tissues, suitable for high-throughput DNA sequencing on the Illumina platform.
authors.affiliation: Department of Nematology, Center for Disease Vector Research, Center for Plant Cell Biology, University of California, Riverside, CA, USA.
authors.forename: Hagop S
authors.initials: HS
authors.lastname: Atamian
authors.forename: Isgouhi
authors.initials: I
authors.lastname: Kaloshian
mesh_terms: D000818:Animals; D001042:Aphids / Q000235:genetics; D002021:Buffers; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000737:chemistry; D015723:Gene Library; D017344:Genes, Insect; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D059367:RNA Cleavage; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012469:Salivary Glands / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D002021:Buffers; D018076:DNA, Complementary; D012333:RNA, Messenger; D012313:RNA; D003851:Deoxyribonucleases