title: Using electrophoretic exclusion to manipulate small molecules and particles on a microdevice.
abstract: Electrophoretic exclusion, a novel separations technique that differentiates species in bulk solution using the opposing forces of electrophoretic velocity and hydrodynamic flow, has been adapted to a microscale device. Proof-of-principle experiments indicate that the device was able to exclude small particles (1 μm polystyrene microspheres) and fluorescent dye molecules (rhodamine 123) from the entrance of a channel. Additionally, differentiation of the rhodamine 123 and polystyrene spheres was demonstrated. The current studies focus on the direct observation of the electrophoretic exclusion behavior on a microchip.
authors.affiliation: Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.
authors.forename: Stacy M
authors.initials: SM
authors.lastname: Kenyon
authors.forename: Noah G
authors.initials: NG
authors.lastname: Weiss
authors.forename: Mark A
authors.initials: MA
authors.lastname: Hayes
mesh_terms: D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods*; D008863:Microspheres; D010316:Particle Size; D011137:Polystyrenes / Q000302:isolation & purification; D020112:Rhodamine 123 / Q000302:isolation & purification
chemical_list: D011137:Polystyrenes; D020112:Rhodamine 123