title: Eukaryotic protein recruitment into the Chlamydia inclusion: implications for survival and growth.
abstract: Chlamydia trachomatis (Ct) is an obligate intracellular human pathogen that multiplies within a parasitophorous vacuole called an inclusion. We report that the location of several host-cell proteins present in the cytosol, the nucleus, and membranes was altered during Ct development. The acyl-CoA synthetase enzyme ACSL3 and the soluble acyl-CoA binding protein ACBD6 were mobilized from organelle membranes and the nucleus, respectively, into the lumen of the inclusion. The nuclear protein ZNF23, a pro-apoptosis factor, was also translocated into the inclusion lumen. ZNF23, among other proteins, might be targeted by Ct to inhibit host cell apoptosis, thereby enabling bacterial survival. In contrast, the acyl-CoA:lysophosphatidylcholine acyltransferase LPCAT1, an endoplasmic reticulum membrane protein, was recruited to the inclusion membrane. The coordinated action of ACBD6, ACSL3 and LPCAT1 likely supports remodeling and scavenging of host lipids into bacterial-specific moieties essential to Ct growth. To our knowledge, these are the first identified host proteins known to be intercepted and translocated into the inclusion.
authors.affiliation: Center for Sickle Cell Disease and Thalassemia, Children's Hospital Oakland Research Institute, Oakland, California, United States of America. esoupene@chori.org
authors.forename: Eric
authors.initials: E
authors.lastname: Soupene
authors.forename: James
authors.initials: J
authors.lastname: Rothschild
authors.forename: Frans A
authors.initials: FA
authors.lastname: Kuypers
authors.forename: Deborah
authors.initials: D
authors.lastname: Dean
mesh_terms: D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000378:metabolism*; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D002690:Chlamydia Infections / Q000378:metabolism*; D002692:Chlamydia trachomatis / Q000378:metabolism*; D003066:Coenzyme A Ligases / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism* / Q000382:microbiology; D021381:Protein Transport; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C530903:ACBD6 protein, human; D018528:ATP-Binding Cassette Transporters; D014157:Transcription Factors; C464650:ZNF23 protein, human; D008243:1-Acylglycerophosphocholine O-Acyltransferase; C526202:Lpcat1 protein, human; D003066:Coenzyme A Ligases