title: Labeling of anti-MUC-1 binding single chain Fv fragments to surface modified upconversion nanoparticles for an initial in vivo molecular imaging proof of principle approach.
abstract: In vivo optical Imaging is an inexpensive and highly sensitive modality to investigate and follow up diseases like breast cancer. However, fluorescence labels and specific tracers are still works in progress to bring this promising modality into the clinical day-to-day use. In this study an anti-MUC-1 binding single-chain antibody fragment was screened, produced and afterwards labeled with newly designed and surface modified NaYF(4):Yb,Er upconversion nanoparticles as fluorescence reporter constructs. The MUC-1 binding of the conjugate was examined in vitro and in vivo using modified state-of-the-art small animal Imaging equipment. Binding of the newly generated upconversion nanoparticle based probe to MUC-1 positive cells was clearly shown via laser scanning microscopy and in an initial proof of principal small animal optical imaging approach.
authors.affiliation: Department of Chemistry, University of Osnabrück, Barbarastr. 7, 46069 Osnabrück, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Hischemöller
authors.affiliation: Department of Chemistry, University of Osnabrück, Barbarastr. 7, 46069 Osnabrück, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Walter
authors.affiliation: Philips Research Laboratories Aachen, Weisshausstr. 2, 52066 Aachen, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Weiler
authors.affiliation: Philips Research Laboratories Aachen, Weisshausstr. 2, 52066 Aachen, Germany.
authors.forename: Helga
authors.initials: H
authors.lastname: Hummel
authors.affiliation: Frauenhofer IME, Fockenbeckstr. 6, 52074 Aachen, Germany.
authors.forename: Theo
authors.initials: T
authors.lastname: Thepen
authors.affiliation: Frauenhofer IME, Fockenbeckstr. 6, 52074 Aachen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Huhn
authors.affiliation: Frauenhofer IME, Fockenbeckstr. 6, 52074 Aachen, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Barth
authors.affiliation: Bayer Technology Services GmbH, Building E41, 51368 Leverkusen, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Hoheisel
authors.affiliation: Bayer Technology Services GmbH, Building E41, 51368 Leverkusen, Germany.
authors.forename: Karen
authors.initials: K
authors.lastname: Köhler
authors.affiliation: Bayer Technology Services GmbH, Building E41, 51368 Leverkusen, Germany.
authors.forename: Diana
authors.initials: D
authors.lastname: Dimova-Landen
authors.affiliation: Molecular Imaging Group, Department of Clinical Radiology, University Hospital Münster, Waldeyerstr. 1, 48149 Münster, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Bremer
authors.affiliation: Department of Chemistry, University of Osnabrück, Barbarastr. 7, 46069 Osnabrück, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Haase
authors.affiliation: Molecular Imaging Group, Department of Clinical Radiology, University Hospital Münster, Waldeyerstr. 1, 48149 Münster, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Waldeck
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis / Q000276:immunology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008819:Mice, Nude; D057054:Molecular Imaging / Q000379:methods*; D018396:Mucin-1 / Q000276:immunology*; D053758:Nanoparticles; D009368:Neoplasm Transplantation; D061848:Optical Imaging / Q000379:methods*; D057127:Single-Chain Antibodies / Q000276:immunology; D013194:Staining and Labeling; D014183:Transplantation, Heterologous
chemical_list: D018396:Mucin-1; D057127:Single-Chain Antibodies