title: Acetylcholine receptors enable the transport of rapsyn from the Golgi complex to the plasma membrane.
abstract: The accumulation of acetylcholine receptors (AChRs) at nerve terminals is critical for signal transmission at the neuromuscular junction, and rapsyn is essential for this process. Previous studies suggest that AChRs might direct rapsyn self-clusters to the synapse. In vivo experiments with fluorescently tagged AChR or rapsyn in zebrafish larvae revealed that rapsyn self-clusters separate from AChRs did not exist before synapse formation. Examination of rapsyn in the AChR-less mutant sofa potato revealed that rapsyn in the absence of AChR was localized in the Golgi complex. Expression of muscle-type AChR in sofa potato restored synaptic clustering of rapsyn, while neuronal type AChR had no effect. To determine whether this requirement of protein interaction is reciprocal, we examined the mutant twitch once, which has a missense mutation in rapsyn. While the AChRs distributed nonsynaptically on the plasma membrane in twitch once, mutant rapsyn was retained in the Golgi complex. We conclude that AChRs enable the transport of rapsyn from the Golgi complex to the plasma membrane through a molecule-specific interaction.
authors.affiliation: Section on Model Synaptic Systems, Laboratory of Molecular Physiology, NIAAA, NIH, Bethesda, MD 20892-9411, USA.
authors.forename: Jee-Young
authors.initials: JY
authors.lastname: Park
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ikeda
authors.forename: Takanori
authors.initials: T
authors.lastname: Ikenaga
authors.forename: Fumihito
authors.initials: F
authors.lastname: Ono
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002462:Cell Membrane / Q000378:metabolism*; D005260:Female; D006056:Golgi Apparatus / Q000378:metabolism*; D008297:Male; D057054:Molecular Imaging / Q000379:methods; D009124:Muscle Proteins / Q000378:metabolism*; D009154:Mutation; D021381:Protein Transport; D011950:Receptors, Cholinergic / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D009124:Muscle Proteins; D011950:Receptors, Cholinergic; D029961:Zebrafish Proteins; C051609:peripheral membrane protein 43K