title: Evaluation of the New NucliSENS EasyQ KPC test for rapid detection of Klebsiella pneumoniae carbapenemase genes (blaKPC).
abstract: KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioMérieux EasyQ real-time PCR assay for bla(KPC) detection with 300 members of the Enterobacteriaceae, including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring bla(KPC) genes, with no false positives, and results were available within 2 h.
authors.affiliation: Institutes of Microbiology, Catholic University of the Sacred Heart, Rome, Italy. tspanu@rm.unicatt.it
authors.forename: Teresa
authors.initials: T
authors.lastname: Spanu
authors.forename: Barbara
authors.initials: B
authors.lastname: Fiori
authors.forename: Tiziana
authors.initials: T
authors.lastname: D'Inzeo
authors.forename: Giulia
authors.initials: G
authors.lastname: Canu
authors.forename: Serena
authors.initials: S
authors.lastname: Campoli
authors.forename: Tommaso
authors.initials: T
authors.lastname: Giani
authors.forename: Ivana
authors.initials: I
authors.lastname: Palucci
authors.forename: Mario
authors.initials: M
authors.lastname: Tumbarello
authors.forename: Maurizio
authors.initials: M
authors.lastname: Sanguinetti
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Rossolini
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase