title: Proteomic analysis of matrix of dental biofilm formed under dietary carbohydrate exposure.
abstract: To evaluate whether protein changes in extracellular matrix of dental biofilm are a unique property of sucrose, this in situ study was conducted using as active control glucose and fructose, the sucrose monosaccharide constituents. Proteins were analyzed by two-dimensional electrophoresis followed by LC-MS/MS after trypsin digestion. Absence or lower abundance of calcium-binding proteins and higher abundance of prolactin-induced proteins were found in biofilm formed in the presence of sucrose or its monosaccharide constituents compared with water, the negative control group. The data suggest that besides sucrose, other dietary carbohydrates may also provoke a change in the protein profile of extracellular matrix of dental biofilm formed.
authors.affiliation: Piracicaba Dental School, University of Campinas, Piracicaba, Brazil.
authors.forename: G P
authors.initials: GP
authors.lastname: Moi
authors.forename: J A
authors.initials: JA
authors.lastname: Cury
authors.forename: T C D
authors.initials: TC
authors.lastname: Dombroski
authors.forename: B A
authors.initials: BA
authors.lastname: Pauletti
authors.forename: A F
authors.initials: AF
authors.lastname: Paes Leme
mesh_terms: D000328:Adult; D018441:Biofilms / Q000187:drug effects; D002135:Calcium-Binding Proteins / Q000032:analysis; D040502:Calgranulin B / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis; D002853:Chromatography, Liquid; D018592:Cross-Over Studies; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D016326:Extracellular Matrix Proteins / Q000032:analysis* / Q000187:drug effects; D005632:Fructose / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007525:Isoelectric Focusing; D026901:Membrane Transport Proteins; D020543:Proteome / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000032:analysis; D013395:Sucrose / Q000494:pharmacology; D053719:Tandem Mass Spectrometry; D014357:Trypsin; D014867:Water
chemical_list: D002135:Calcium-Binding Proteins; D040502:Calgranulin B; D002352:Carrier Proteins; D004040:Dietary Carbohydrates; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D026901:Membrane Transport Proteins; C488892:PIP protein, human; D020543:Proteome; D012471:Salivary Proteins and Peptides; D014867:Water; D005632:Fructose; D013395:Sucrose; D014357:Trypsin; D005947:Glucose