title: Preservation of viscoelastic properties of rabbit vocal folds after implantation of hyaluronic Acid-based biomaterials.
abstract: OBJECTIVE
  To compare the rheological characteristics of structurally different hyaluronic acid (HA)-based biomaterials that are presently used for phonosurgery and to investigate their influence on the viscoelastic properties of vocal folds after implantation in an in vivo rabbit model.
  
  
  METHODS
  In vitro and in vivo rheometric investigation.
  
  
  METHODS
  Experimental laboratory, Inha and Seoul National Universities.
  
  
  METHODS
  Viscoelastic shear properties of 3 HA-based biomaterials (Rofilan, Restylane, and Reviderm) were measured with a strain-controlled rheometer. These biomaterials were injected into the deep layers of rabbit vocal folds, and viscoelastic moduli of the injected vocal folds were determined 2 months after the injection. The vocal fold specimens were observed using a light microscope and a transmission electron microscope.
  
  
  RESULTS
  All HA-based biomaterials showed similar levels of shear viscosity, which were slightly higher than that of human vocal folds reported in previous studies. Compared with noninjected control vocal folds, there were no significant differences in the magnitudes of both elastic shear modulus (G') and viscous modulus (G") of injected vocal folds among all of the materials. Light microscopic images showed that all materials were observed in the deep layers of vocal folds and electron scanning images revealed that injected HA particles were homogeneously distributed in regions of collagenous fibers.
  
  
  CONCLUSIONS
  HA-based biomaterials could preserve the viscoelastic properties of the vocal folds, when they were injected into vocal folds in an in vivo rabbit model. However, further studies on the influence of the biomaterials on the viscoelasticity of human vocal folds in ECM surroundings are still needed.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Inha University School of Medicine, Incheon, Korea.
authors.forename: Jeong-Seok
authors.initials: JS
authors.lastname: Choi
authors.forename: Nahn Ju
authors.initials: NJ
authors.lastname: Kim
authors.forename: Sarah
authors.initials: S
authors.lastname: Klemuk
authors.forename: Yun Ho
authors.initials: YH
authors.lastname: Jang
authors.forename: In Suh
authors.initials: IS
authors.lastname: Park
authors.forename: Kyung Hyun
authors.initials: KH
authors.lastname: Ahn
authors.forename: Jae-Yol
authors.initials: JY
authors.lastname: Lim
authors.forename: Young-Mo
authors.initials: YM
authors.lastname: Kim
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D004548:Elasticity; D006820:Hyaluronic Acid / Q000031:analogs & derivatives*; D007267:Injections; D046529:Microscopy, Electron, Transmission; D019736:Prostheses and Implants; D011817:Rabbits; D012212:Rheology; D033081:Shear Strength; D014783:Viscosity; D014827:Vocal Cords / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*
chemical_list: D001672:Biocompatible Materials; D006820:Hyaluronic Acid; C445361:Restylane