title: [The effect of glutoxim on Na+ transport in frog skin: the role of cytoskeleton].
abstract: Using voltage-clamp technique, the possible role of the cytoskeleton in the effect of pharmacological analogue of oxidized glutathione (GSSG), drug glutoxim, on Na+ transport in the frog Rana temporaria skin was investigated. It was shown for the first time that preincibation of the skin with the microtubular disrupter, nocodazole, actin filament disrupter, cytochalasin D or protein phosphatase PP1/PP2A inhibitor, calyculin A, significantly decrease the stimulatory effect of glutoxim on Na+ transport. The data suggest the involvement of microtubules and microfilaments in the regulatory effect of glutoxim on Na+ transport in frog skin and that reorganization of actin filaments or microtubules leads to inhibition of stimulatory effect of glutoxim on Na+ transport in frog skin epithelia.
authors.forename: A V
authors.initials: AV
authors.lastname: Mel'nitskaia
authors.forename: Z I
authors.initials: ZI
authors.lastname: Krutetskaia
authors.forename: O E
authors.initials: OE
authors.lastname: Lebedev
authors.forename: S N
authors.initials: SN
authors.lastname: Butov
authors.forename: N I
authors.initials: NI
authors.lastname: Krutetskaia
authors.forename: V G
authors.initials: VG
authors.lastname: Antonov
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D004220:Disulfides / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology; D008297:Male; D008387:Marine Toxins; D008870:Microtubules / Q000187:drug effects / Q000502:physiology*; D015739:Nocodazole / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology*; D010080:Oxazoles / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D011896:Rana temporaria; D012867:Skin / Q000187:drug effects*; D012964:Sodium / Q000378:metabolism*; D053778:Thiazolidines / Q000494:pharmacology
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D004220:Disulfides; D008387:Marine Toxins; D009842:Oligopeptides; D010080:Oxazoles; D053778:Thiazolidines; C462699:glutoxim; D015638:Cytochalasin D; C059041:calyculin A; D012964:Sodium; C037068:latrunculin B; D015739:Nocodazole