title: Characterization of Foxp3 gene from grass carp (Ctenopharyngodon idellus): a rapamycin-inducible transcription factor in teleost immune system.
abstract: In this study, we cloned grass carp foxp3 (gcfoxp3) gene including 5' flanking region and determined its expression profiles in vivo under immunosuppressive conditions. Sequence analysis revealed that the promoter of gcfoxp3 contains AP-1, AML-1/Runx1, NF-κb and GATA-3 binding sites, which positively or negatively regulate mammalian foxp3 expression. In addition, the intron II of gcfoxp3 contains some putative binding sites including AP-1, NFAT, Smad3, RAR, CREB/ATF and FOXO1, which are corresponding to their locations in the proximal intronic enhancers of human foxp3. In an in vivo model of grass carp, an immunosuppressive agent rapamycin was showed to stimulate gcfoxp3 mRNA expression in thymus, gill, head kidney and spleen after bacterial challenge. Moreover, rapamycin increased gcFoxp3 protein levels with an additive manner in the infected fish. These findings support the involvement of fish Foxp3 in immune response and highlight a possible signaling pathway that regulates teleost Foxp3 expression.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.
authors.forename: Mu
authors.initials: M
authors.lastname: Yang
authors.forename: He
authors.initials: H
authors.lastname: Wei
authors.forename: Taiqiang
authors.initials: T
authors.lastname: Zhao
authors.forename: Xinyan
authors.initials: X
authors.lastname: Wang
authors.forename: Anying
authors.initials: A
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D020123:Sirolimus / Q000494:pharmacology
chemical_list: C418974:FOXP3 protein, human; D029941:Fish Proteins; D051858:Forkhead Transcription Factors; D020123:Sirolimus