title: Picornaviruses in cerebrospinal fluid of children with meningitis in Luanda, Angola.
abstract: Human enteroviruses are the most common cause of viral meningitis. Viral-bacterial interaction may affect the clinical course and outcome of bacterial meningitis. In Africa, viruses might be responsible for 14-25% of all meningitis cases. However, only few studies from Africa have reported detection of viruses in the cerebrospinal fluid (CSF) or mixed viral-bacterial infections of the central nervous system (CNS). The aim of the present study was to investigate the presence of picornaviruses in the CSF of children suffering from meningitis in Luanda, Angola. The study included 142 consecutive children enrolled in a prospective study of bacterial meningitis in Luanda between 2005 and 2006, from whom a CSF sample was available. CSF samples were obtained at hospital admission, stored in a deep-freeze, and transported to Finland for testing by real-time PCR for picornaviruses. Enteroviruses were detected in 4 (3%) of 142 children with presumed bacterial meningitis. A 5-month-old girl with rhinovirus and Haemophilus influenzae meningitis recovered uneventfully. An 8-year-old girl with human enterovirus and pneumococcal meningitis developed no sequelae. A 2-month-old girl with human enterovirus and malaria recovered quickly. A 7-month-old girl with human enterovirus was treated for presumed tuberculous meningitis and survived with severe sequelae. Mixed infections of the CNS with picornaviruses and bacteria are rare. Detection of an enterovirus does not affect the clinical picture and outcome of bacterial meningitis.
authors.affiliation: Pediatric Hospital David Bernardino, Luanda, Angola. tuulapelkonen@hotmail.com
authors.forename: Tuula
authors.initials: T
authors.lastname: Pelkonen
authors.forename: Irmeli
authors.initials: I
authors.lastname: Roine
authors.forename: Elizabete
authors.initials: E
authors.lastname: Anjos
authors.forename: Svetlana
authors.initials: S
authors.lastname: Kaijalainen
authors.forename: Merja
authors.initials: M
authors.lastname: Roivainen
authors.forename: Heikki
authors.initials: H
authors.lastname: Peltola
authors.forename: Anne
authors.initials: A
authors.lastname: Pitkäranta
mesh_terms: D000810:Angola / Q000453:epidemiology; D002555:Cerebrospinal Fluid / Q000821:virology*; D002648:Child; D060085:Coinfection / Q000453:epidemiology / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D008587:Meningitis, Viral / Q000453:epidemiology* / Q000821:virology*; D010849:Picornaviridae / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000453:epidemiology* / Q000821:virology*; D011446:Prospective Studies