title: Fluorescence in situ hybridization of three oncogenes on a leiomyoma.
abstract: Using fluorescence in situ hybridization technique, expression of three oncogenes, C-myc, RARa, and cyclin-D was tested on a uterine leiomyoma. C-myc and RARa were amplified in approximately 30% and 90% of the cells, respectively. Numerous small signals of C-myc were indicative of the presence of double minutes. Amplification of RARa is being reported for the first time in a leiomyoma. Cyclin-D was normal in diploid cells while it was highly amplified in polyploid cells. Low levels of amplified C-myc and cyclin-D cells seem to be the reason for this tumor to be benign, while RARa could not be effective without the association of some other gene such as PML. Information presented here are significant toward developing new curative strategies such as gene-specific drugs and molecular manipulation to stop the activity of cancer gene. Further study may elucidate that how fibroids grow and maintain their rare benign nature.
authors.affiliation: Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Crozer-Chester Medical Center, Upland, Pennsylvania 19013 USA. shamim@zebraglobal.net
authors.forename: Shamim Ahmad
authors.initials: SA
authors.lastname: Faruqi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Saquib
authors.forename: Christopher
authors.initials: C
authors.lastname: Harsch
authors.forename: Joel S
authors.initials: JS
authors.lastname: Noumoff
mesh_terms: D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D056741:Cyclin D / Q000235:genetics / Q000378:metabolism*; D004276:DNA, Satellite / Q000235:genetics; D005260:Female; D018628:Gene Dosage; D016259:Genes, myc; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007889:Leiomyoma / Q000378:metabolism / Q000473:pathology*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism; D000072482:Retinoic Acid Receptor alpha; D015533:Transcriptional Activation
chemical_list: D056741:Cyclin D; D004276:DNA, Satellite; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; C000607071:RARA protein, human; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha