title: Advanced adipose-derived stem cell protein extracts with antioxidant activity modulates matrix metalloproteinases in human dermal fibroblasts.
abstract: Advanced adipose-derived stem cell protein extracts (AAPE) were used instead of live stem cells to investigate their effect on oxidative stress and matrix metalloproteinases (MMPs) related to tissue repair in human dermal fibroblasts (HDFs). In this study, it was observed that AAPE at 2μg/ml specifically exhibited scavenging activity of hydrogen peroxide and reducing power. The inhibitory effect of AAPE at 2μg/ml on MMP-2 activity was increased in the presence of phorbol myristate acetate (PMA). In the absence of PMA, AAPE significantly enhanced activities of MMP-1 and MMP-2 in HDFs, respectively. However, the level of MMP-1 expression was decreased in a dose dependent manner by AAPE. In addition, while the level of extracellular signal-regulated kinases 1 (ERK1) activation was reduced in the presence of AAPE compared to blank, the level that of ERK2 activation was not changed. The expression level of c-Fos, a part of activator protein-1 (AP-1), was increased in nucleus of HDFs. These results reveal that activation of MMPs in the presence of AAPE was increased via AP-1 in HDFs, suggesting that AAPE can be a potential candidate for tissue repair.
authors.affiliation: Department of Chemistry, Dong-Eui University, Busan 614-714, Republic of Korea.
authors.forename: Yong-Byung
authors.initials: YB
authors.lastname: Chae
authors.affiliation: Department of Biomaterial Control, Dong-Eui University, Busan 614-714, Republic of Korea.
authors.forename: Jae Seol
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Dong-Eui University, Busan 614-714, Republic of Korea.
authors.forename: Hye-Jung
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Department of Chemistry, Dong-Eui University, Busan 614-714, Republic of Korea.
authors.forename: In-Hwan
authors.initials: IH
authors.lastname: Park
authors.affiliation: Department of Chemistry, Dong-Eui University, Busan 614-714, Republic of Korea. Electronic address: mmkim@deu.ac.kr.
authors.forename: Moon-Moo
authors.initials: MM
authors.lastname: Kim
authors.affiliation: Prostemics Research Institute, Seoul, Republic of Korea.
authors.forename: Ye-Hyoung
authors.initials: YH
authors.lastname: Park
authors.affiliation: Prostemics Research Institute, Seoul, Republic of Korea.
authors.forename: Dong-Soo
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Department of Biomaterial Control, Dong-Eui University, Busan 614-714, Republic of Korea; Department of Biotechnology and Bioengineering, Dong-Eui University, Busan 614-714, Republic of Korea; Department of Blue-Bio Regional Innovation Center, Dong-Eui University, Busan 614-714, Republic of Korea. Electronic address: jonghwanlee@deu.ac.kr.
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D002457:Cell Extracts / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D012867:Skin / Q000166:cytology; D013234:Stem Cells; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002457:Cell Extracts; D009687:Nuclear Proteins; D018808:Transcription Factor AP-1; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1