title: Tricine-SDS-PAGE.
abstract: Tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) is an efficient way of separating low-molecular-mass proteins. However, the standard system is quite complicated and specifically may not be useful when the separated proteins require to be recovered from the gel for quantitative analysis. Here, we describe a simplified system whereby these smaller proteins can be resolved in comparatively low-percentage gels which have high compatibility with modern detectors such as UV and inductively coupled plasma mass spectrometry (ICP-MS).
authors.affiliation: Loughborough University, Loughborough, UK.
authors.forename: Syed R
authors.initials: SR
authors.lastname: Haider
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Reid
authors.forename: Barry L
authors.initials: BL
authors.lastname: Sharp
mesh_terms: D002021:Buffers; D002364:Caseins / Q000737:chemistry / Q000302:isolation & purification*; D004396:Coloring Agents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods* / Q000592:standards; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D059748:Proteolysis; D012015:Reference Standards; D012394:Rosaniline Dyes / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D002021:Buffers; D002364:Caseins; D004396:Coloring Agents; D011506:Proteins; D012394:Rosaniline Dyes; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C004692:coomassie Brilliant Blue; D005998:Glycine; C100184:tricine