The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast.
We report the isolation of Pi1, a gene conferring broad-spectrum resistance to rice blast (Magnaporthe oryzae). Using loss- and gain-of-function approaches, we demonstrate that Pi1 is an allele at the Pik locus. Like other alleles at this locus, Pi1 consists of two genes. A functional nucleotide polymorphism (FNP) was identified that allows differentiation of Pi1 from other Pik alleles and other non-Pik genes. A extensive germplasm survey using this FNP reveals that Pi1 is a rare allele in germplasm collections and one that has conferred durable resistance to a broad spectrum of pathogen isolates.
State Key Laboratory for Conservation and Utilization of Subtropic Agrobioresources, Laboratory of Plant Resistance and Genetics, College of Natural Resources and Environmental Sciences, South China Agricultural University, Guangzhou 510642, China.
Lixia
L
Hua
Jianzhong
J
Wu
Caixia
C
Chen
Weihuai
W
Wu
Xiuying
X
He
Fei
F
Lin
Li
L
Wang
Ikuo
I
Ashikawa
Takashi
T
Matsumoto
Ling
L
Wang
Qinghua
Q
Pan
D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics*; D020082:Magnaporthe / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*
D018744:DNA, Plant