TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro.
Trefoil Factor Family (TFF) plays an essential role in the intestinal epithelial restitution, but the relationship between TFF1 and gastric cancer (GC) is still unclear. The present study aimed to determine the role of TFF1 in repairing gastric mucosa and in the pathogenesis of GC.  The TFF1 expression in different gastric mucosas was measured with immunohistochemistry. Then, siRNA targeting TFF1 or plasmids expressing TFF1 gene were transfected into BGC823 cells, SGC7901 cells and GES-1 cells. The cell proliferation was detected with MTT assay and apoptosis and cell cycle measured by flow cytometry. From normal gastric mucosa to mucosa with dysplasia and to gastric cancer, the TFF1 expression had a decreasing trend. Down-regulation of TFF1 expression significantly reduced the apoptosis of three cell lines and markedly facilitated their proliferation but had no significant effect on cell cycle. Over-expression of TFF1 could promote apoptosis of three cell lines and inhibit proliferation but had no pronounced effect on cell cycle. TFF1 can inhibit proliferation and induce apoptosis of GC cells in vitro.
Department of Gastroenterology, Tongji Hospital, Tongji University, Shanghai 200065, China.
Yanli
Y
Ge
Junjie
J
Zhang
Jianchun
J
Cao
Qiong
Q
Wu
Longe
L
Sun
Likun
L
Guo
Zhirong
Z
Wang
D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004396:Coloring Agents; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D010957:Plasmids / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis / Q000235:genetics*
D004396:Coloring Agents; D034741:RNA, Small Interfering; C051838:TFF1 protein, human; D013778:Tetrazolium Salts; D013844:Thiazoles; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins; C022616:thiazolyl blue