Differences in mitochondrial  gene expression profiles, enzyme activities and myosin heavy chain types in yak  versus bovine skeletal muscles.
Hypoxia  can affect energy metabolism. We examined gene expression and enzyme activity  related to mitochondrial energy metabolism, as well as myosin heavy chain (MyHC)  types in yaks (Bos grunniens) living at high altitudes. Real-time  quantitative PCR assays indicated that the yak has significantly lower levels  of carnitine palmitoyltransferase (CPT) mRNA in the biceps femoris and  lower levels of uncoupling protein 3 (UCP3) mRNA in both biceps femoris  and longissimus dorsi than in Yellow cattle. No significant differences between  yak and Yellow cattle were observed in the activities of mitochondrial β-hydroxyacyl-CoA dehydrogenase,  isocitrate dehydrogenase and cytochrome oxidase in the same muscles.  Semi-quantitative RT-PCR analysis showed that the MyHC 1 mRNA levels in  yak biceps femoris was lower than in Yellow cattle. We conclude that the yak  has significantly lower mRNA levels of CPT, UCP3, and MyHC 1 in  biceps femoris than in Yellow cattle, suggesting that the yak biceps femoris  has lower fatty acid oxidation capacity and greater glycolytic metabolic  potential.
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, P.R. China.
Y Q
YQ
Lin
Y O
YO
Xu
Y
Y
Yue
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Jin
Y
Y
Qu
F
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Dong
Y P
YP
Li
Y C
YC
Zheng
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics / Q000378:metabolism; D002417:Cattle / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D050259:Genes, Mitochondrial / Q000235:genetics*; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008297:Male; D008931:Mitochondria, Muscle / Q000201:enzymology*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology*; D018995:Myosin Heavy Chains / Q000235:genetics* / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071247:Uncoupling Protein 3
D007473:Ion Channels; D024101:Mitochondrial Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D000071247:Uncoupling Protein 3; D015094:3-Hydroxyacyl CoA Dehydrogenases; D007521:Isocitrate Dehydrogenase; D011451:Prostaglandin-Endoperoxide Synthases; D002334:Carnitine O-Palmitoyltransferase; D018995:Myosin Heavy Chains