[Transmembrane transport of K+ and Cl- during pollen grain activation in vivo and in vitro].
We studied the possibility of K+ and Cl- efflux from tobacco pollen grains during their activation in vitro or on the stigma of a pistil. For this purpose the X-ray microanalysis and spectrofluorometry were applied. We found that the relative content of potassium and chlorine in the microvolume of pollen grain decreases during its hydration and activation on stigma. Efflux of these ions was found both in vivo and in vitro. In model in vitro experiments anion channel inhibitor NPPB ((5-nitro-2-(3-phenylpropylamino) benzoic acid) in the concentration that was blocking pollen germination, reduced Cl- efflux; potassium channel inhibitor (tetraethylammonium chloride) partially reduced K+ efflux and lowered the percent of activated cells. Another blocker of potassium channels Ba2+ caused severe decrease in cell volume and blocked the activation. In general, the obtained data demonstrates that the initiation of pollen germination both in vivo and in vitro involves the activation of K+ and Cl- release. An important role in these processes is played by NPPB-, TEA- and Ba(2+)-sensitive plasmalemma ion channels.
M A
MA
Breĭgina
N P
NP
Matveeva
D S
DS
Andreiuk
I P
IP
Ermakov
D001692:Biological Transport; D048429:Cell Size; D018118:Chloride Channels / Q000037:antagonists & inhibitors; D002712:Chlorides / Q000378:metabolism*; D004577:Electron Probe Microanalysis; D007477:Ions; D009579:Nitrobenzoates / Q000494:pharmacology; D011058:Pollen / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism; D019789:Tetraethylammonium / Q000494:pharmacology; D014026:Nicotiana / Q000378:metabolism
D018118:Chloride Channels; D002712:Chlorides; D007477:Ions; D009579:Nitrobenzoates; D015221:Potassium Channels; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D019789:Tetraethylammonium; D011188:Potassium