Chronic unpredictable mild stress affects myocardial metabolic profiling of SD rats.
Depression is frequently comorbid with cardiovascular diseases (CVDs), but a full understanding of the mechanisms is still on its way. Chronic unpredictable mild stress (CUMS) model is a commonly used model to mimic clinical depression, here we present a GC/MS-based metabolic profiling approach to investigate myocardial metabolic changes of CUMS SD rats. Principal Component Analysis (PCA) and Partial Least Squares-Discriminant Analysis (PLS-DA) were utilized to reveal differences between the model and control group. This study found that molecules proved cardioprotective involving glutamine (P=0.019) and inosine (P=0.013), fatty acid (9,12-octadecadienoic acid, P=0.002; hexadecanoic acid, P=0.006; octadecanoic acid, P=0.030) which serve as the major energy source of heart and collagen molecule precursor proline (P=0.036) had down-regulated in CUMS model group.
Clinical Pharmaceutical Research Institute, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
Wen-yuan
WY
Zhang
Shao
S
Liu
Huan-de
HD
Li
Hua-lin
HL
Cai
D000818:Animals; D001522:Behavior, Animal; D015415:Biomarkers / Q000378:metabolism; D001835:Body Weight; D002318:Cardiovascular Diseases / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control; D002908:Chronic Disease; D003094:Collagen / Q000378:metabolism; D003863:Depression / Q000150:complications / Q000175:diagnosis / Q000378:metabolism* / Q000523:psychology; D004195:Disease Models, Animal; D004734:Energy Metabolism; D008401:Gas Chromatography-Mass Spectrometry; D016018:Least-Squares Analysis; D008297:Male; D055432:Metabolomics / Q000379:methods; D009206:Myocardium / Q000378:metabolism*; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D013315:Stress, Psychological / Q000150:complications / Q000175:diagnosis / Q000378:metabolism* / Q000523:psychology
D015415:Biomarkers; D003094:Collagen