Langkolide, a 32-membered macrolactone antibiotic produced by Streptomyces sp. Acta 3062.
A new 32-membered macrolactone antibiotic, named langkolide, was isolated from the mycelium of Streptomyces sp. Acta 3062. The langkolide structure was determined by HR-MS and 1D and 2D NMR as a 32-membered macrolactone connected from an overhanging polyketide tail to a naphthoquinone unit mediated by two carbohydrate moieties. The producing strain was isolated from a rhizosphere soil of Clitorea sp. collected at Burau Bay, Langkawi, Malaysia, and was characterized by its morphological and chemotaxonomic features in addition to its 16S rRNA gene sequence. It was identified as a member of the Streptomyces galbus clade. Langkolide exhibited various bioactivities including antimicrobial and antiproliferative activities. Furthermore, langkolide inhibited human recombinant phosphodiesterase 4 with an IC(50) value of 0.48 μM.
Institut für Chemie, Technische Universität Berlin, 10623 Berlin, Germany.
Soleiman E
SE
Helaly
Andreas
A
Kulik
Heidi
H
Zinecker
Kamalanathan
K
Ramachandaran
Geok Yuan Annie
GY
Tan
Johannes F
JF
Imhoff
Roderich D
RD
Süssmuth
Hans-Peter
HP
Fiedler
Vikineswary
V
Sabaratnam
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008296:Malaysia; D015394:Molecular Structure; D025282:Mycelium / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D058988:Phosphodiesterase 4 Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013302:Streptomyces / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D018942:Macrolides; D058988:Phosphodiesterase 4 Inhibitors; C574823:langkolide