Urinary total arsenic and 8-hydroxydeoxyguanosine are associated with renal cell carcinoma in an area without obvious arsenic exposure.
8-Hydroxydeoxyguanosine (8-OHdG) is one of the most reliable and abundant markers of DNA damage. The study was designed to explore the relationship between urinary 8-OHdG and renal cell carcinoma (RCC) and to investigate whether individuals with a high level of 8-OHdG would have a modified odds ratio (OR) of arsenic-related RCC. This case-control study was conducted with 132 RCC patients and 245 age- and sex-matched controls from a hospital-based pool between November 2006 and May 2009. Pathological verification of RCC was completed by image-guided biopsy or surgical resection of renal tumors. Urinary 8-OHdG levels were determined using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Concentrations of urinary arsenic species, including inorganic arsenic, monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA), were determined by a high performance liquid chromatography-linked hydride generator and atomic absorption spectrometry. Level of urinary 8-OHdG was significantly associated with the OR of RCC in a dose-response relationship after multivariate adjustment. Urinary 8-OHdG was significantly related to urinary total arsenic. The greatest OR (3.50) was seen in the individuals with high urinary 8-OHdG and high urinary total arsenic. A trend test indicated that the OR of RCC was increased with one of these factors and was further increased with both (p=0.002). In conclusion, higher urinary 8-OHdG was a strong predictor of the RCC. High levels of 8-OHdG combined with urinary total arsenic might be indicative of arsenic-induced RCC.
Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Chao-Yuan
CY
Huang
Chien-Tien
CT
Su
Chi-Jung
CJ
Chung
Yeong-Shiau
YS
Pu
Jan-Show
JS
Chu
Hsiu-Yuan
HY
Yang
Chia-Chang
CC
Wu
Yu-Mei
YM
Hsueh
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000367:Age Factors; D001151:Arsenic / Q000652:urine*; D002292:Carcinoma, Renal Cell / Q000139:chemically induced* / Q000453:epidemiology / Q000652:urine; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D003404:Creatinine / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000139:chemically induced* / Q000453:epidemiology / Q000652:urine; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D012737:Sex Factors; D013624:Taiwan / Q000453:epidemiology; D053719:Tandem Mass Spectrometry
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003404:Creatinine; D003849:Deoxyguanosine; D001151:Arsenic