Stem cell markers aldehyde dehydrogenase type 1 and nestin expressions in renal cell cancer.
OBJECTIVE
To identify stem cell markers aldehyde dehydrogenase type 1 (ALDH-1) and nestin expression in renal cell carcinoma (RCC).


METHODS
95 RCC patients who had radical or partial nephrectomy were included in this study. Normal renal tissue samples of the same patients were designated as the control group. Paraffin blocks of RCC patients were studied immunohistochemically. Expressions were analyzed semiquantitively. Relation of tumor grade and stage with these expressions was determined. Results were calculated with Mann Whitney U and Kruskal Wallis tests.


RESULTS
There were 55 male (57.8%) and 40 (42.2%) female patients in the study. Age and sex of the patients were not correlated with their antibody expressions (p > 0.05). ALDH-1 and nestin expression was higher in cancer tissues than normal tissues (p < 0.05). Nestin expression in renal cencer tissue was inversely related with tumor stage (p < 0.05) but there was no relation with Fuhrman grade. ALDH-1 expression was correlated with tumor grade (p < 0.05) but not with tumor stage (p > 0.05).


CONCLUSIONS
ALDH-land nestin expression have a role in RCC pathophysiology. There is a need to perform more studies about stem cell markers in RCC with more patients and in these future studies lymph node metastasis, distant organ metastasis and survival rates should be included.
Okmeydani Training and Research Hospital, 1st Urology Clinic, Istanbul, Turkey. ozbekemin@hotmail.com
Emin
E
Ozbek
Gokhan
G
Calik
Alper
A
Otunctemur
Tamer
T
Aliskan
Suleyman
S
Cakir
Murat
M
Dursun
Adnan
A
Somay
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000080924:Aldehyde Dehydrogenase 1 Family; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D005260:Female; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009392:Nephrectomy / Q000379:methods; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D064231:Nestin; D011237:Predictive Value of Tests; D011379:Prognosis; D050697:Retinal Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013234:Stem Cells / Q000378:metabolism*
D014408:Biomarkers, Tumor; D007381:Intermediate Filament Proteins; D007527:Isoenzymes; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase