[Evaluation on glucose-xylose co-fermentation by a recombinant Zymomonas mobilis strain].
Co-fermentation of glucose and xylose is critical for cellulosic ethanol, as xylose is the second most abundant sugar in lignocellulosic hydrolysate. In this study, a xylose-utilizing recombinant Zymomonas mobilis TSH01 was constructed by gene cloning, and ethanol fermentation of the recombinant was evaluated under batch fermentation conditions with a fermentation time of 72 h. When the medium containing 8% glucose or xylose, was tested, all glucose and 98.9% xylose were consumed, with 87.8% and 78.3% ethanol yield, respectively. Furthermore, the medium containing glucose and xylose, each at a concentration of 8%, was tested, and 98.5% and 97.4% of glucose and xylose was fermented, with an ethanol yield of 94.9%. As for the hydrolysate of corn stover containing 3.2% glucose and 3.5% xylose, all glucose and 92.3% xylose were consumed, with an ethanol yield of 91.5%. In addition, monopotassium phosphate can facilitate the consumption of xylose and enhance ethanol yield.
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China.
Quanzhou
Q
Feng
Shizhong
S
Li
Li
L
Wang
Tiancheng
T
Li
D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D011995:Recombination, Genetic; D014994:Xylose / Q000378:metabolism*; D017355:Zymomonas / Q000235:genetics / Q000378:metabolism*
D000431:Ethanol; D014994:Xylose; D005947:Glucose