Reclamation of ampicillin sensitivity for the genetic manipulation of Legionella pneumophila.
Research on Legionella pneumophila, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of L. pneumophila lacking loxA, a chromosomally encoded β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting Legionella strains to ampicillin sensitivity and conditions for utilizing bla as a selectable marker.
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.
Molly C
MC
Sutherland
Joseph P
JP
Vogel
D000667:Ampicillin / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005818:Genetic Engineering; D016952:Legionella pneumophila / Q000235:genetics* / Q000254:growth & development; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
D015415:Biomarkers; D000667:Ampicillin; D001618:beta-Lactamases