Nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/chitosan scaffolds for skin regeneration.
The purpose of this study was to evaluate hybrid poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/chitosan nanofibrous mats as scaffolds for skin engineering. In vitro studies were carried out to test the potential of the scaffolds for fibroblasts adhesion, viability, and proliferation (L929 cell line). The in vivo performance was also studied in a full-thickness wound healing model. PHBV/chitosan 4:1 (w/w) exhibited a higher in vitro biocompatibility and a better ability for cell adhesion and growth, compared to PHBV/chitosan 2:3 (w/w). The in vivo assay also revealed the better performance of this scaffold, improving the wound healing process in rats.
QOPNA Research Unit, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Beatriz
B
Veleirinho
Daniela S
DS
Coelho
Paulo F
PF
Dias
Marcelo
M
Maraschin
Rosa M
RM
Ribeiro-do-Valle
José A
JA
Lopes-da-Silva
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D012038:Regeneration / Q000187:drug effects*; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000293:injuries; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects; D000094122:Polyhydroxybutyrates
D001672:Biocompatible Materials; D048271:Chitosan; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates