[Simultaneous determination of isoorientin, scutellarin and cynaroside in rat plasma and pharmacokinetics by UPLC-MS/MS].
OBJECTIVE
To establish a UPLC-MS/MS analysical method for simultaneous determination of concentrations of isoorientin, scutellarin and cynaroside in rat plasma and to study their pharmacokinetic characteristics after intravenous injection of 3 doses of Fufang Hongcao in rats.


METHODS
Acidified plasma samples were precipitated for protein with methanol. Waters Acquity BEH C18 column was adopted for spectrum, with mobile phase as 0. 1% formic acid acetonitrile-0. 1% formic acid-water gradient elution. Detection was carried out by the multiple reaction monitoring (MRM) positive ion mode with ESI ionization source.


RESULTS
Three flavonoids show a good linear relationship, with the extraction recovery ranging between 78.56% and 101.91% and a high intra-and inter-day precisions and accuracy. The MRT of the three flavonoids were all lower than 22 min in rats.


CONCLUSIONS
The above men tioned method is so specific, rapid, sensitive that it is suitable for pharmacokinetic studies of Fufang Hongcao injection in rats.
School of Pharmacy, Guiyang Medical Collage, Guiyang 550004, China.
Yong
Y
Huang
Feng
F
He
Lin
L
Zheng
Zhirong
Z
Zhang
Yanyu
Y
Lan
Yonglin
Y
Wang
D000818:Animals; D047310:Apigenin / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005260:Female; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics; D005965:Glucuronates / Q000097:blood / Q000493:pharmacokinetics; D047311:Luteolin / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D005965:Glucuronates; C484876:scutellarin; D047310:Apigenin; C066408:luteolin-7-glucoside; C057912:homoorientin; D047311:Luteolin