[Influence of metal ions and specific chemical reagents on activity of alpha-L-rhamnosidase of Eupenicillium erubescens].
The effect of cations, anions and specific chemical reagents: 1-[3-(dimethylamino) propyl]-3-ethylcarbodiimide methiodide, EDTA, o-phenanthroline, dithiotreitol, L-cysteine, beta-mercaptoethanol, p-chlormercurybenzoate (p-ChMB), N-ethylmaleimide on the activity of alpha-L-rhamnosidase of Eupenicillium erubescens has been investigated. The essential role of Ag+ and Hg2+ which inhibit the alpha-L-rhamnosidase activity by 47-73% has been shown. Whereas L-cysteine exhibits the protective effect, rhamnose in concentration of 1-5 mM does not protect the enzyme from negative effect of Ag+ and Hg2+. Basing on the inhibitory and kinetic analysis it was supposed that the carboxyl group of C-terminal aminoacid and imidazole group of histidine take part in the catalytic action of alpha-L-rhamnosidase. It was assumed that sulphydryl groups took part in catalysis, carried out by alpha-L-rhamnosidase of E. erubescens, since the activity of alpha-L-rhamnosidase inhibited by p-ChMB and thiol reagents such as dithiothreitol, L-cysteine, beta-mercaptoethanol did not remove its inhibitory action.
E V
EV
Gudzenko
N V
NV
Borzova
L D
LD
Varbanets
D055162:Biocatalysis / Q000187:drug effects; D002413:Cations, Divalent / Q000494:pharmacology*; D002414:Cations, Monovalent / Q000494:pharmacology*; D003124:Colorimetry; D055324:Eupenicillium / Q000201:enzymology*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D008628:Mercury / Q000633:toxicity; D008751:Methylene Blue / Q000494:pharmacology; D009596:Nitrophenols / Q000378:metabolism; D055668:Photochemical Processes; D012210:Rhamnose / Q000378:metabolism; D012834:Silver / Q000633:toxicity; D012996:Solutions; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D013816:Thermodynamics
D002413:Cations, Divalent; D002414:Cations, Monovalent; D005656:Fungal Proteins; D009596:Nitrophenols; D012996:Solutions; D013439:Sulfhydryl Reagents; D012834:Silver; D006026:Glycoside Hydrolases; C022525:alpha-L-rhamnosidase; D008628:Mercury; D012210:Rhamnose; D008751:Methylene Blue; C024836:4-nitrophenol