General and electrophysiological toxic effects of manganese in rats following subacute administration in dissolved and nanoparticle form.
In an attempt to model occupational and environmental Mn exposures and their possible interaction, young male Wistar rats were exposed to Mn by oral administration in dissolved form (MnCl(2)·4H(2)O, 14.84 and 59.36 mg/kg b.w.) and by intratracheal application of MnO(2) nanoparticles (2.63 mg/kg b.w.). After 3 and 6 weeks oral, or 3 weeks oral plus 3 weeks intratracheal, exposure, general toxicological, and electrophysiological tests were done. Body weight gain was significantly reduced after 6 and 3 plus 3 weeks exposure, but the effect of the latter on the pace of weight gain was stronger. Organ weights signalized systemic stress and effect on lungs. Changes in evoked electrophysiological responses (cortical sensory evoked potential and nerve action potential) indicated that the 3 plus 3 weeks combined exposure caused equal or higher changes in the latency of these responses than 6 weeks of exposure, although the calculated summed Mn dose in the former case was lower. The results showed the importance of the physicochemical form of Mn in determining the toxic outcome, and suggested that neurofunctional markers of Mn action may indicate the human health effect better than conventional blood Mn measurement.
Department of Public Health, University of Szeged Faculty of Medicine, Dóm tér 10, 6720 Szeged, Hungary.
Edina
E
Horváth
Zsuzsanna
Z
Máté
Szabolcs
S
Takács
Péter
P
Pusztai
András
A
Sápi
Zoltán
Z
Kónya
László
L
Nagymajtényi
András
A
Papp
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004594:Electrophysiology; D008297:Male; D008345:Manganese / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D008345:Manganese