[Chemical ablation of the gallbladder with sodium hypochlorite in an animal model].
BACKGROUND
The cholecystectomy is the current treatment of the biliary lithiasis. Nevertheless there have been attempts in order to eliminate the gallbladder epithelium and to generate the sclerosis of the organ using chemical substances, heat and laser.


OBJECTIVE
Sodium hypochlorite and fibrin glue is proposed to achieve the ablation of the gallbladder mucosa and sclerosis.


METHODS
Thirty rabbits were divided into three groups of 10. Accessing surgically to the gallbladder, sodium hypochlorite was injected, afterward the same was done with fibrin glue to occlude the cystic duct and collapse the organ lumen, avoiding the bile reflux. In the control groups ethanol plus fibrin adhesive and physiological solution was used. In day 65 the animals were sacrificed and the result of the procedure was observed macroscopic and histologically.


RESULTS
Eight of the gallbladders treated with sodium hypochlorite disappeared leaving a small subhepatic scar. There were no intra nor postoperative complications. In the group of the ethanol the result was successful only in one case, and with saline solution there was no ablation. A significant difference exists for the group treated with sodium hypochlorite plus fibrin glue compared to the ethanol plus fibrin adhesive group (p <0,0055) and the saline solution group (p <0,0007).


CONCLUSIONS
In this experimental model there was possible the ablation of the gallbladder using sodium hypochlorite as sclerosing agent and fibrin glue to collapse the lumen and to occlude the cystic duct.
Unidad Hospitalaria de Cirugía No 1, Hospital Nacional de Clínicas, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina. joverclos@hotmail.com
Rafael J
RJ
Jover Clos
Juan P
JP
Álamo
Mónica
M
Matsuzaki
Maria E
ME
Dionisio de Caballier
Héctor F
HF
Bustos
Luis
L
Gramatica
D000818:Animals; D003549:Cystic Duct / Q000187:drug effects*; D005704:Gallbladder / Q000187:drug effects* / Q000473:pathology; D008297:Male; D023421:Models, Animal; D011817:Rabbits; D012973:Sodium Hypochlorite / Q000008:administration & dosage*
D012973:Sodium Hypochlorite