Real-time PCR detection of enteric viruses in source water and treated drinking water in Wuhan, China.
A total of 48 water samples were collected from six water treatment plants in Wuhan and analyzed by real-time PCR assay for viral identification of enterovirus (EV), rotavirus group A (RVA), human adenovirus (HAdV) as well as human adenovirus subgroup F (HAdVF) during the period from December 2010 to October 2011. HAdV, HAdVF, and RVA were all positively detected in the samples of source water and treated drinking water. EV could be found in 46 % (11/24) of all the source water samples, but only 21 % (5/24) positive in treated drinking water. The concentrations of these three kinds of enteric viruses detected were as follows: HAdV > RVA > EV. The highest removal rate was EV (97 %), followed by RVA (82 %), HAdV (73 %), and HAdVF (72 %). HAdV and RVA have been abundant in untreated river water and finished water after conventional processes of water treatment plants, while bacterial indicators could not be detected in tap water, which met the standard of China for drinking water bacterial quality. Some factors that could affect the accuracy of qPCR detection are also discussed in this study.
MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Xiao Yan
XY
Ye
Xing
X
Ming
Yong Lu
YL
Zhang
Wen Qing
WQ
Xiao
Xia Ning
XN
Huang
Yu Guang
YG
Cao
Kang Ding
KD
Gu
D000256:Adenoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000704:Analysis of Variance; D002681:China; D004269:DNA, Bacterial; D060766:Drinking Water / Q000821:virology*; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis*
D004269:DNA, Bacterial; D060766:Drinking Water