Genome-wide identification and characterization of  simple sequence repeat loci in grape phylloxera, Daktulosphaira vitifoliae.
A genome-wide sequence search was conducted to  identify simple sequence repeat (SSR) loci in phylloxera, Daktulosphaira  vitifoliae, a major grape pest throughout the world. Collectively,  1524 SSR loci containing mono-, di-, tri-, tetra-, penta-, and hexanucleotide  motifs were identified. Among them, trinucleotide repeats were the most  abundant in the phylloxera genome (34.4%), followed by hexanucleotide (20.4%)  and dinucleotide (19.6%) repeats. Mono-, tetra- and pentanucleotide repeats  were found at a frequency of 1.3, 11.2 and 12.9%, respectively. The abundance  and inherent variations in SSRs provide valuable information for developing  molecular markers. The high levels of allelic variation and codominant features  of SSRs make this marker system a useful tool for genotyping, diversity  assessment and population genetic studies of reproductive characteristics of  phylloxera in agricultural and natural populations.
Crop Diseases, Pests and Genetics Research Unit, USDA-ARS, Parlier, CA, USA. Hong.Lin@ars.usda.gov
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D005828:Genetics, Population; D018745:Genome, Plant / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D027843:Vitis / Q000235:genetics*