Bioassay-guided isolation of novel compound from Paeonia suffruticosa Andrews roots as an IL-1β inhibitor.
The inhibition of Interleukin-1beta (IL-1β) is of substantial interest for the treatment of rheumatoid arthritis. Using an in vitro assay with RAW 264.7 cells, oxo-acetic acid 2-ethoxy-4-(3-hydroxy-2-oxopropyl) phenyl ester (1) was isolated from the roots of Paeonia suffruticosa Andrews as an inhibitor of IL-1β with an IC(50) value of 56 μM. Compound 1 is a novel phenylesteric compound from P. suffruticosa Andrews. Compound 1 was shown to inhibit the production of pro-inflammatory cytokines in RAW 264.7 cells. Thus, a possible new action of novel compound is provided explaining the anti-rheumatoid arthritic properties of P. suffruticosa Andrews.
Life Science R&D Center, Sinil Pharmaceutical Co., Ltd., Chungju, Korea.
Yun-Hyeok
YH
Choi
Hee-Jung
HJ
Yoo
Ill Chan
IC
Noh
Jeong-Min
JM
Lee
Jae Won
JW
Park
Wahn Soo
WS
Choi
Jung Ho
JH
Choi
D000085:Acetates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019342:Acetic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000096:Acetone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D018501:Antirheumatic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors* / Q000502:physiology; D051379:Mice; D029596:Paeonia; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018517:Plant Roots
D000085:Acetates; D018501:Antirheumatic Agents; D007166:Immunosuppressive Agents; D053583:Interleukin-1beta; D010936:Plant Extracts; C579252:oxoacetic acid 2-ethoxy-4-(3-hydroxy-2-oxopropyl) phenyl ester; D000096:Acetone; D019342:Acetic Acid