Three-dimensional imaging of fetal mouse kidneys.
Three-dimensional imaging is a valuable tool for analyzing kidney growth and development. This technique provides information about spatial relationships between the branching ureteric bud, nephrons, and other structures within the kidney. Availability of user-friendly volume-rendering software now puts this technique within the capability of most laboratories with access to a confocal microscope. This paper describes how to prepare samples and acquire images and three-dimensional volume-rendered images.
Department of Medicine, Baylor College of Medicine, Houston, TX, USA. Hyink@bcm.edu
Deborah
D
Hyink
D000818:Animals; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007668:Kidney / Q000254:growth & development / Q000648:ultrastructure*; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D012984:Software; D013194:Staining and Labeling / Q000379:methods