Sesquiterpene lactones from Inula hupehensis inhibit nitric oxide production in RAW264.7 macrophages.
Phytochemical investigation of the aerial parts of Inula hupehensis Ling. led to the isolation and identification of 27 sesquiterpene lactones (1-27), including three new eudesmanolides (3-5), three new germacranolides (9-11), one new xanthanolide (16), two new carabrone derivatives (25-26), and 18 known sesquiterpene lactones. The structures were elucidated by extensive spectroscopic methods and comparison to previously reported spectroscopic data. All compounds were evaluated for their inhibitory effects against LPS-induced nitric oxide production in RAW264.7 macrophages, and compound 5 showed the strongest activity with the IC₅₀ value of 3.2 ± 0.4 µM.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, PR China.
Jiang-Jiang
JJ
Qin
Jia-Xian
JX
Zhu
Qi
Q
Zeng
Xiang-Rong
XR
Cheng
Shou-De
SD
Zhang
Hui-Zi
HZ
Jin
Wei-Dong
WD
Zhang
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020128:Inhibitory Concentration 50; D038143:Inula / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000009:adverse effects; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045788:Sesquiterpenes, Germacrane / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007783:Lactones; D008070:Lipopolysaccharides; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; C476916:eudesmanolide; C432449:germacranolide; D009569:Nitric Oxide