[Study on mechanism of ginsenoside Rg1-induced human neural stem cells differentiation by genechip].
OBJECTIVE
The molecular targets of ginsenoside Rg1-induced neural stem cells (NSCs) differentiation were screened by genechip.


METHODS
7th day following ginsenoside Rg1 induced human neural stem cells to neurons the gene expression was observed by genechip. The purpose gene and signal transduction pathways were selected by the data calculations, and then confirmed by western blot and immunohistochemical method.


RESULTS
7th day following Rg1-induced NSCs differentiation, there were about 675 different genes, 255 genes of which were up-regulated and 420 genes down-regulated obviously. Meanwhile the ERK1/2 (extracellular signal-regulated protein kinase) in MAPK (mitogen-activated protein kinase) pathway was related with the NSCs differentiation. The Western blot and immunohistochemistry detection confirmed that ERK 1/2 protein and its phosphorylation were significantly increased, which can be blocked by PD98059 (ERK1/2 inhibitor). In addition, differentiation rate of NSCs was also decreased obviously in ginsenoside Rg1-induced differentiated NSCs when ERK blocker PD98059 was used.


CONCLUSIONS
ERK1/2 is an important molecular target in ginsenoside Rg1-induced NSC differentiation. The selected differentially expressed genes by genechip may provide new clues to study of ginsenoside Rg1-induced NSCs differentiation.
Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China.
Xiangqin
X
Zhao
Yingbo
Y
Li
Yinghong
Y
Jiang
Di
D
Chen
Rong
R
Jiang
Shali
S
Wang
D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000235:genetics; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D013997:Time Factors
D005419:Flavonoids; D036145:Ginsenosides; D047428:Protein Kinase Inhibitors; C035054:ginsenoside Rg1; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one