Selection of peptides for  serological detection of equine infectious anemia.
Equine  infectious anemia caused by equine infectious anemia virus is an important  disease due to its high severity and incidence in animals. We used a phage  display library to isolate peptides that can be considered potential markers  for equine infectious anemia diagnosis. We selected peptides using IgG purified  from a pool comprised of 20 sera from animals naturally infected with equine  infectious anemia virus. The diagnostic potential of these peptides was  investigated by ELISA, Western blot and dot blot with purified IgG and serum  samples. Based on the results, we chose a peptide mimetic for glycoprotein gp45  epitopes of equine infectious anemia virus, with potential for use as an  antigen in indirect diagnostic assays. Synthesis of this peptide has possible  applications for the development of new diagnostic tools for this disease.
Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil. elmaira27@yahoo.com.br
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Santos
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Cardoso
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Souza
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Goulart
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Heinemann
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Leite
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Reis
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D019295:Computational Biology; D004797:Enzyme-Linked Immunosorbent Assay; D004859:Equine Infectious Anemia / Q000097:blood* / Q000175:diagnosis*; D006736:Horses / Q000097:blood* / Q000821:virology*; D007074:Immunoglobulin G / Q000276:immunology; D004860:Infectious Anemia Virus, Equine / Q000302:isolation & purification; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry
D007074:Immunoglobulin G; D019151:Peptide Library; D010455:Peptides