The effect of the microgravity rotating culture system on the chondrogenic differentiation of bone marrow mesenchymal stem cells.
We investigated the influence of the microgravity rotating culture system on the chondrogenic differentiation of bone marrow mesenchymal stem cells (MSCs). During chondrogenic induction, MSCs combined with polyglycolic acid (PGA) were cultured by static culture or microgravity rotating culture and chondrocyte formation was confirmed by toluidine blue staining. Furthermore, the mRNA and protein expressions of a specific cartilage extracellular matrix protein (collagen type II and Aggrecan) were evaluated by real-time RT-PCR and western blot, respectively. Toluidine blue staining indicated the OD values of proteoglycans semi-determination were higher in the microgravity rotating culture group than the static culture group. Following chondrogenic induction, mRNA and proteins of collagen type II and Aggrecan were more significantly expressed in cells of the microgravity rotating culture group compared with the controls. Compared with routine three-dimensional static culture, the microgravity rotating culture system was more effective for the construction of tissue-engineered cartilage in vitro.
Department of Orthopaedics, Tongji University School of Medicine, Shanghai, China. wuxinggood@sina.cn
Xing
X
Wu
Shao-hua
SH
Li
Lie-ming
LM
Lou
Zheng-rong
ZR
Chen
D053674:Aggrecans / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002356:Cartilage / Q000378:metabolism / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D020219:Chondrogenesis / Q000502:physiology*; D024043:Collagen Type II / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D064235:Matrilin Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D011100:Polyglycolic Acid / Q000378:metabolism; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D014893:Weightlessness
D053674:Aggrecans; D024043:Collagen Type II; D016326:Extracellular Matrix Proteins; D064235:Matrilin Proteins; D011100:Polyglycolic Acid