[Study on compatibility extraction technology of Rhei Radix et Rhizoma, Scutellariae Radix and Phellodendri Chinensis Cortex].
OBJECTIVE
To determine the optimum compatibility extraction technology of Rhei Radix et Rhizoma, Scutellariae Radix and Phellodendri Chinensis Cortex.


METHODS
The drugs were combined respectively firstly, then use HPLC to determinate extraction rates of evaluating indicators including emodin, chrysophano, baicalin and berberine hydrochloride. Orthogonal experiment was used to detect the effect on concentration and volume of ethanol, times of extraction.


RESULTS
The extraction rates of evaluating indicators were relatively high in combined extraction of Rhei Radix et Rhizoma with Scutellariae Radix and single extraction of Phellodendri Chinensis Cortex. The optimized combined extraction were 12 times more than the volume of 60% ethanol solution, by boiling for 3 times, each for 1.0 hour, and that of single extraction were 10 times more than the volume of 60% ethanol solution, by boiling for 3 times, each for 1.0 hour.


CONCLUSIONS
The optimized compatibility extraction technology is reasonable, stable and practical.
Zhejiang Chinese Medical University, Hangzhou 310053, China. aizhenzhou@sina.com
Aizhen
A
Zhou
Hua
H
Yin
Zhiqing
Z
Wang
D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D032102:Phellodendron / Q000737:chemistry*; D031364:Scutellaria baicalensis / Q000737:chemistry*; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D000431:Ethanol