A phylogenetic analysis of normal modes evolution in enzymes and its relationship to enzyme function.
Since the dynamic nature of protein structures is essential for enzymatic function, it is expected that functional evolution can be inferred from the changes in protein dynamics. However, dynamics can also diverge neutrally with sequence substitution between enzymes without changes of function. In this study, a phylogenetic approach is implemented to explore the relationship between enzyme dynamics and function through evolutionary history. Protein dynamics are described by normal mode analysis based on a simplified harmonic potential force field applied to the reduced C(α) representation of the protein structure while enzymatic function is described by Enzyme Commission numbers. Similarity of the binding pocket dynamics at each branch of the protein family's phylogeny was analyzed in two ways: (1) explicitly by quantifying the normal mode overlap calculated for the reconstructed ancestral proteins at each end and (2) implicitly using a diffusion model to obtain the reconstructed lineage-specific changes in the normal modes. Both explicit and implicit ancestral reconstruction identified generally faster rates of change in dynamics compared with the expected change from neutral evolution at the branches of potential functional divergences for the α-amylase, D-isomer-specific 2-hydroxyacid dehydrogenase, and copper-containing amine oxidase protein families. Normal mode analysis added additional information over just comparing the RMSD of static structures. However, the branch-specific changes were not statistically significant compared to background function-independent neutral rates of change of dynamic properties and blind application of the analysis would not enable prediction of changes in enzyme specificity.
Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA.
Jason
J
Lai
Jing
J
Jin
Jan
J
Kubelka
David A
DA
Liberles
D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030562:Databases, Protein; D004798:Enzymes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D056004:Molecular Dynamics Simulation; D010802:Phylogeny; D011485:Protein Binding; D000516:alpha-Amylases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D004798:Enzymes; D000429:Alcohol Oxidoreductases; C083779:2-hydroxyacid dehydrogenase; D006631:Amine Oxidase (Copper-Containing); D000516:alpha-Amylases