Association of GSTM1 and GSTT1 with ageing in auto  repair shop workers.
We evaluated the possible influence of  glutathione S-transferase mu (GSTM1) and glutathione S-transferase theta  (GSTT1) genes on genetic damage due to occupational exposure, which  contributes to accelerate ageing. This study was conducted on 120 car auto  repair workshop workers exposed to occupational hazards and 120 controls  without this kind of exposure. The null and non-null genotypes of GSTM1 and  GSTT1 genes were determined by multiplex PCR. Micronucleus frequency, Comet  tail length and relative telomere length differences between the null and  non-null genotypes of the GSTM1 gene were significantly greater in the exposed  group. Lack of GSTT1 did not affect the damage biomarkers significantly (P >  0.05), while lack of GSTM1 was associated with greater susceptibility to  genomic damage due to occupational exposure. It was concluded that early ageing  is under the influence of these genes and the environmental and  socio-demographic factors. Duration of working time was significantly  associated with micronucleus frequency, Comet tail length and relative telomere  length.
Institute of Gerontology, Universiti Putra Malaysia, Selangor, Malaysia.
S A
SA
Eshkoor
S J
SJ
Marashi
P
P
Ismail
S A
SA
Rahman
M
M
Mirinargesi
M Y
MY
Adon
R V
RV
Devan
D000375:Aging / Q000235:genetics / Q000502:physiology*; D020552:Comet Assay; D004249:DNA Damage / Q000235:genetics; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D060885:Multiplex Polymerase Chain Reaction; D016273:Occupational Exposure / Q000009:adverse effects*
D005982:Glutathione Transferase; C413545:glutathione S-transferase T1