Endocytoscopy allows accurate in vivo differentiation of mucosal inflammatory cells in IBD: a pilot study.
BACKGROUND
Precise activity assessment of inflammatory bowel disease (IBD) is essential to determine extent and severity of disease for optimized therapy. Despite ongoing developments in endoscopy, final diagnosis still relies on the interpretation of histopathological features. Recently, endocytoscopy (EC) was introduced as a new endoscopic imaging modality, enabling in vivo microscopic imaging within the mucosal layer of the gut at a magnification up to 1400-fold. The aim of our study was to determine the reliability of EC for the discrimination of mucosal inflammatory cells and intestinal inflammatory disease activity in patients with IBD.


METHODS
In all, 40 patients with IBD (Crohn's disease n = 19; ulcerative colitis n = 21) who underwent colonoscopy were prospectively included in this study. Methylene blue or toluidine blue was topically applied to enable EC. Data were digitally saved and analyzed blinded to clinical and endoscopic data.


RESULTS
EC enabled visualization of different histopathological features. Based on these specifications, it was possible to reliably distinguish single inflammatory cells by EC with the following respective sensitivities and specificities: neutrophilic (60% and 95%), basophilic (74.43% and 94.44%), eosinophilic granulocytes (75% and 90.48%), and lymphocytes (88.89% and 93.33%). Interobserver agreement between two investigators was substantial (kappa 0.61-0.78), while intraobserver agreement was substantial to almost perfect (kappa 0.76-0.88). Concordance between EC and histopathology for grading intestinal disease activity was 100%.


CONCLUSIONS
EC enabled the detection and discrimination of single mucosal inflammatory cells and inflammatory disease activity. Therefore, this technique has the potential to improve both in vivo diagnosis and clinical management of IBD patients.
Department of Medicine I, University of Erlangen-Nuremberg, Germany.
Helmut
H
Neumann
Michael
M
Vieth
Markus F
MF
Neurath
Raja
R
Atreya
D000328:Adult; D000368:Aged; D003093:Colitis, Ulcerative / Q000473:pathology; D003106:Colon / Q000473:pathology*; D003113:Colonoscopy / Q000295:instrumentation / Q000379:methods*; D004396:Coloring Agents; D003424:Crohn Disease / Q000473:pathology; D005260:Female; D006098:Granulocytes / Q000473:pathology; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000473:pathology*; D007413:Intestinal Mucosa / Q000473:pathology*; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D008751:Methylene Blue; D008875:Middle Aged; D015588:Observer Variation; D010865:Pilot Projects; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D016037:Single-Blind Method; D014048:Tolonium Chloride
D004396:Coloring Agents; D014048:Tolonium Chloride; D008751:Methylene Blue