Roche genome sequencer FLX based high-throughput sequencing of ancient DNA.
Since the development of so-called "next generation" high-throughput sequencing in 2005, this technology has been applied to a variety of fields. Such applications include disease studies, evolutionary investigations, and ancient DNA. Each application requires a specialized protocol to ensure that the data produced is optimal. Although much of the procedure can be followed directly from the manufacturer's protocols, the key differences lie in the library preparation steps. This chapter presents an optimized protocol for the sequencing of fossil remains and museum specimens, commonly referred to as "ancient DNA," using the Roche GS FLX 454 platform.
Centre for GeoGenetics, Natural History Museum of Denmark, Copenhagen, Denmark. dalquezar@snm.ku.dk
David E
DE
Alquezar-Planas
Sarah L
SL
Fordyce
D000818:Animals; D018070:Biological Specimen Banks; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D053938:DNA Fragmentation; D053476:Extinction, Biological; D015723:Gene Library; D016678:Genome; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods; D006801:Humans; D056665:Mammoths / Q000235:genetics; D059125:Neanderthals / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods; D001503:Ursidae / Q000235:genetics
D004247:DNA