Ag(I) ion mediated formation of a C-A mispair by DNA polymerases.
Silver turns up the A-C: In the presence of Ag(I) ions, a DNA polymerase incorporated deoxyadenosine (from dATP) at the site opposite cytosine in the template strand to afford the full-length product (see scheme), meaning that DNA polymerases prefer a C-Ag(I)-A base pair to the more thermodynamically stable C-Ag(I)-C base pair.
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Tatsuya
T
Funai
Yuki
Y
Miyazaki
Megumi
M
Aotani
Eriko
E
Yamaguchi
Osamu
O
Nakagawa
Shun-ichi
S
Wada
Hidetaka
H
Torigoe
Akira
A
Ono
Hidehito
H
Urata
D001483:Base Sequence; D002412:Cations / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D012834:Silver / Q000737:chemistry*
D002412:Cations; D012834:Silver; D004259:DNA-Directed DNA Polymerase