Methods for the determination of plasma or tissue glutathione levels.
We present two different methods for determining levels of glutathione in complex biological samples and plasma. The DTNB/GR enzyme recycling method is sensitive and requires no specialized equipment. The HPLC method is particularly useful for situations in which sample amounts are limited. Detailed instructions for performing each method as well as the advantages and disadvantages of each are discussed in this chapter.
Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, Columbus, OH, USA. tipple.10@osu.edu
Trent E
TE
Tipple
Lynette K
LK
Rogers
D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D005978:Glutathione / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism; D019803:Glutathione Disulfide / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D005980:Glutathione Reductase / Q000737:chemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D010084:Oxidation-Reduction; D012015:Reference Standards
D004228:Dithionitrobenzoic Acid; D005980:Glutathione Reductase; D005978:Glutathione; D019803:Glutathione Disulfide