MicroRNA-27a promotes myoblast proliferation by targeting myostatin.
MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that play critical roles in skeletal muscle development as well as in regulation of muscle cell proliferation and differentiation. However, the role of miRNAs in myoblast proliferation remains poorly understood. Here we found that the expression of miR-27a was increased during proliferation of C2C12 myoblasts. Moreover, overexpression of miR-27a in C2C12 cells promoted myoblast proliferation by reducing the expression of myostatin, a critical inhibitor of skeletal myogenesis. In addition, the miR-27a was confirmed to target myostatin 3'UTR by a luciferase reporter analysis. Together, these results suggest that miR-27a promotes myoblast proliferation through targeting myostatin.
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan 625014, PR China.
Zhiqing
Z
Huang
Xiaoling
X
Chen
Bing
B
Yu
Jun
J
He
Daiwen
D
Chen
D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000502:physiology*; D055435:Myostatin / Q000235:genetics*
D020413:3' Untranslated Regions; D035683:MicroRNAs; C540674:Mirn27 microRNA, mouse; D055435:Myostatin