Bisphenol A-mediated suppression of LPL gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells.
The endocrine disrupting chemical, bisphenol A (BPA), has been shown to accelerate the rate of adipogenesis and increase the amount of triglyceride accumulation during differentiation of 3T3-L1 preadipocytes. The objective of this study was to investigate if that observation is mirrored in human primary cells. Here we investigated the effect of BPA on adipogenesis in cultured human primary adult stem cells. Continuous exposure to BPA throughout the 14 days of differentiation dramatically reduced triglyceride accumulation and suppressed gene transcription of the lipogenic enzyme, lipoprotein lipase (LPL). Results presented in the present study show for the first time that BPA can reduce triglyceride accumulation during adipogenesis by attenuating the expression of LPL gene transcription. Also, by employing image cytometric analysis rather than conventional Oil red O staining techniques we show that BPA regulates triglyceride accumulation in a manner which does not appear to effect adipogenesis per se.
Department of Biochemistry, Faculty of Life Sciences, National University of Ireland Galway, Galway, Ireland. c.linehan1@gmail.com
Chris
C
Linehan
Sanjeev
S
Gupta
Afshin
A
Samali
Lynn
L
O'Connor
D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D001559:Benzhydryl Compounds; D015415:Biomarkers; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D008071:Lipoprotein Lipase / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D047495:PPAR gamma / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014280:Triglycerides / Q000378:metabolism*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D015415:Biomarkers; D008071:Lipoprotein Lipase; D047495:PPAR gamma; D010636:Phenols; D014280:Triglycerides; C006780:bisphenol A; D000098826:Bisphenol A Compounds