Fontibacter ferrireducens sp. nov., an Fe(III)-reducing bacterium isolated from a microbial fuel cell.
A novel, dissimilatory Fe(III)-reducing bacterium, designated strain SgZ-2(T), which could couple glucose oxidation to iron reduction for energy conservation, was isolated from a microbial fuel cell. The isolate was Gram-staining-negative, catalase-positive, oxidase-negative and facultatively anaerobic. The strain was able to grow on tryptic soy agar at 15-37 °C and in the presence of 0-5 % (w/v) NaCl. The predominant cellular fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C17 : 1ω9c; the major respiratory quinone was MK-7; the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and three other unidentified lipids; and the DNA G+C content was 55.3 mol%. Phylogenetic analyses based on 16S rRNA sequences showed that the novel strain was most closely related to Fontibacter flavus CC-GZM-130(T) (99.9 % sequence similarity), and was a member of the family Cyclobacteriaceae. The levels of DNA-DNA relatedness observed between strain SgZ-2(T) and F. flavus CCM 7650(T) (<41 %) indicated that the two strains represented two distinct species. Based on phylogenetic analyses and phenotypic characteristics, strain SgZ-2(T) represents a novel species of the genus Fontibacter, for which the name Fontibacter ferrireducens sp. nov. is proposed. The type strain is SgZ-2(T) ( = CCTCC M 2011498(T)  = KACC 16525(T)).
College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, PR China.|Guangdong Institute of Eco-Environment and Soil Sciences, Guangzhou 510650, PR China.
Jun
J
Zhang
Guangdong Institute of Eco-Environment and Soil Sciences, Guangzhou 510650, PR China.
GuiQin
G
Yang
Guangdong Institute of Eco-Environment and Soil Sciences, Guangzhou 510650, PR China.
Shungui
S
Zhou
Guangdong Institute of Eco-Environment and Soil Sciences, Guangzhou 510650, PR China.
Yueqiang
Y
Wang
Guangdong Institute of Eco-Environment and Soil Sciences, Guangzhou 510650, PR China.
Yong
Y
Yuan
Guangdong Institute of Eco-Environment and Soil Sciences, Guangzhou 510650, PR China.
Li
L
Zhuang
D015373:Bacterial Typing Techniques; D001482:Base Composition; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005290:Ferric Compounds / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D005290:Ferric Compounds; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7