Real-time quantitative analysis of the influence of blue light on citrinin biosynthetic gene cluster expression in Monascus.
When Monascus MX was grown under blue light instead of in the dark, citrinin production increased from 478 mg l(-1) to 698 mg l(-1). To explain this, the expression of the pksCT gene, which encodes citrinin polyketide synthase, and of 5 ORFs around it, were monitored. Blue light enhanced citrinin production by upregulating the expression of orf1, orf3, and orf4, indicating that pksCT was not the key gene responsible for the quantity of citrinin production in blue light.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, No. 29, the 13th Avenue, TEDA, Tianjin 300457, People's Republic of China. changluwang2009@gmail.com
Changlu
C
Wang
Hua
H
Yang
Mianhua
M
Chen
Yurong
Y
Wang
Fengjuan
F
Li
Cheng
C
Luo
Shuyi
S
Zhao
Dong
D
He
D053898:Biosynthetic Pathways / Q000235:genetics*; D002953:Citrinin / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects*; D005800:Genes, Fungal; D008027:Light; D032741:Monascus / Q000235:genetics* / Q000528:radiation effects*; D005810:Multigene Family; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
D002953:Citrinin