Zinc supplementation attenuates high glucose-induced epithelial-to-mesenchymal transition of peritoneal mesothelial cells.
Zinc (Zn) plays an important role in preventing many types of epithelial-to-mesenchymal transition (EMT)-driven fibrosis in vivo. But its function in the EMT of the peritoneal mesothelial cells (PMCs) remains unknown. Here, we studied the Zn effect on the high glucose (HG)-induced EMT in the rat PMCs (RPMCs) and the underlying molecular mechanisms. We found that Zn supplementation significantly inhibited TGF-β1 and ROS production, and attenuated the HG-induced EMT in the RPMCs, likely through inhibition of MAPK, NF-κB, and TGF-β/Smad pathways.
Department of Nephrology, the First Affiliated Hospital, China Medical University, 155th Nanjing North Street, Shenyang, Liaoning, 110001, People's Republic of China.
Xiuli
X
Zhang
Jun
J
Wang
Yi
Y
Fan
Lina
L
Yang
Lining
L
Wang
Jianfei
J
Ma
D000818:Animals; D016588:Anticarcinogenic Agents / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D019587:Dietary Supplements; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D005947:Glucose / Q000009:adverse effects; D006943:Hyperglycemia / Q000473:pathology / Q000503:physiopathology*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D009852:Omentum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D010537:Peritoneum / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D015032:Zinc / Q000737:chemistry / Q000378:metabolism*; D019287:Zinc Sulfate / Q000037:antagonists & inhibitors / Q000378:metabolism
D016588:Anticarcinogenic Agents; D015415:Biomarkers; D002614:Chelating Agents; D017382:Reactive Oxygen Species; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D019287:Zinc Sulfate; D005947:Glucose; D015032:Zinc