[Study on functional recovery of hypoxic-ischemic brain injury by Rg1-induced NSCs].
OBJECTIVE
To observe the effect of Rg1-induced NSCs in treatment of neonatal rat model with hypoxiaischemia.


METHODS
The neonatal rat model of HIE was established and assessed by using TTC staining and behavioral observation, then Rg1-induced NSCs was transplanted into the neonatal rat of HIE by lateral ventricle injection. Water maze test and somatosensory evoked potential were detected to observe brain function and the immunohistochemistry was done to assess growth and differentiation about transplanted NSCs a month after transplanted.


RESULTS
The transplantation of Rg1-induced NSCs could significantly shorten incubation period, swimming distance, exploration time of target quadrants of water maze test and incubation period and amplitude of somatosensory evoked potentials. Additionally, the concentrated expression appeared in the hippocampus and grew around the ischemic injury area in transplantation group.


CONCLUSIONS
Transplantation of Rg1-induced NSCs play a better role in the treatment of neonatal HIE rats.
Key Laboratory of Neurobiology, Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China.
Yingbo
Y
Li
Liu
L
Tu
Di
D
Chen
Rong
R
Jiang
Yaping
Y
Wang
Shall
S
Wang
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D005071:Evoked Potentials; D005260:Female; D036145:Ginsenosides / Q000494:pharmacology*; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D018782:Maze Learning; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*
D036145:Ginsenosides; C035054:ginsenoside Rg1