Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for rapid detection of a new SFTS bunyavirus.
The etiological agent of severe fever with thrombocytopenia syndrome (SFTS) is a bunyavirus that was first identified in China in 2009. We have developed and validated a one-step, single-tube, reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of SFTS bunyavirus (SFTSV). This assay demonstrated high specificity and sensitivity, with a detection limit of 10(1) TCID(50) ml(-1). When combined with the fluorescent detection reagent (FDR) method, results could be determined by observing a color change within 30 min. As an accurate, rapid, simple and low-cost diagnostic method, this RT-LAMP assay will be helpful for detecting and preventing further SFTSV infection in China.
Jinan Center for Disease Control and Prevention, Jinan, 250021 Shandong Province, China.
Guoliang
G
Yang
Bing
B
Li
Lanzheng
L
Liu
Weichu
W
Huang
Wen
W
Zhang
Yuandong
Y
Liu
D002044:Bunyaviridae Infections / Q000175:diagnosis* / Q000821:virology; D002681:China; D005453:Fluorescence; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016856:Phlebovirus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors; D014773:Virology / Q000379:methods*