Repeated inhalation of crack-cocaine affects spermatogenesis in young and adult mice.
To investigate the effects of repeated crack-cocaine inhalation on spermatogenesis of pubertal and mature Balb/c mice, ten young (Y(ex)) and ten adult (A(ex)) Balb/c mice were exposed to the smoke from 5 g of crack with 57.7% of pure cocaine in an inhalation chamber, 5 days/week for 2 months. The young (Y(c)) and adult (A(c)) control animals (n = 10) were kept in a specially built and controlled animal house facility. The morphologic analysis of both testes of all animals included the analysis of quantitative and qualitative histologic parameters to assess the effect of crack-cocaine on spermatogenesis and Leydig cells. Apoptosis was determined by immunolabeling with caspase-3 antibodies. Compared to the Y(c) animals, Y(ex) animals showed a significant reduction in the number of stage VII tubules per testis (p = 0.02), Sertoli cells (p < 0.001) and elongated spermatids (p = 0.001). Comparisons between the Y(ex) and A(ex) groups identified a significant reduction in the number of Sertoli cells (p < 0.001) and round spermatids (p < 0.001) in the Y(ex) group and a significant increase in apoptotic Leydig cells (p = 0.04) in the A(ex) group. The experimental results indicate that crack-cocaine smoke inhalation induced spermatogenesis disruption in chronically exposed mice, particularly in pubertal mice.
Reproductive Toxicology Unit, Experimental Air Pollution Laboratory, São Paulo University Medical School, São Paulo, SP, Brazil. adrianapiresbio@gmail.com
A
A
Pires
P
P
Pieri
M
M
Hage
A B G
AB
Santos
M C R
MC
Medeiros
R C T
RC
Garcia
M
M
Yonamine
J
J
Hallak
P H N
PH
Saldiva
J C
JC
Zorzetto
H M S
HM
Bueno
D000280:Administration, Inhalation; D000375:Aging / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016578:Crack Cocaine / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012906:Smoke / Q000009:adverse effects*; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000473:pathology
D016578:Crack Cocaine; D012906:Smoke