Safety and efficacy of an E2 glycoprotein subunit vaccine produced in mammalian cells to prevent experimental infection with bovine viral diarrhoea virus in cattle.
Bovine viral diarrhea (BVD) infection caused by bovine viral diarrhea virus (BVDV), a Pestivirus of the Flaviviridae family, is an important cause of morbidity, mortality and economical losses in cattle worldwide. E2 protein is the major glycoprotein of BVDV envelope and the main target for neutralising antibodies (NAbs). Different studies on protection against BVDV infection have focused on E2, supporting its putative use in subunit vaccines. A truncated version of type 1a BVDV E2 (tE2) expressed in mammalian cells was used to formulate an experimental oleous monovalent vaccine. Immunogenicity was studied through immunisation of guinea pigs and followed by trials in cattle. Calves of 8-12 months were vaccinated, twice with a 4 week interval, with either a tE2 subunit vaccine (n = 8), a whole virus inactivated vaccine (n = 8) or left untreated as negative control group (n = 8). Four weeks after the last immunisation the animals were experimentally challenged intranasally with a non-cythopathic BVDV strain. Following challenge, BVDV was isolated from all unvaccinated animals, while 6 out of 8 animals vaccinated with tE2 showed complete virological protection indicating that the tE2 vaccine presented a similar performance to a satisfactory whole virus inactivated vaccine.
Instituto de Virología, CICVyA, INTA-Castelar, Buenos Aires, Argentina.
Andrea
A
Pecora
María Sol Pérez
MS
Aguirreburualde
Alejandra
A
Aguirreburualde
Maria Rosa
MR
Leunda
Anselmo
A
Odeon
Sebastián
S
Chiavenna
Diego
D
Bochoeyer
Marcelo
M
Spitteler
Jorge L
JL
Filippi
Maria J
MJ
Dus Santos
Susana M
SM
Levy
Andrés
A
Wigdorovitz
D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D004279:DNA, Viral / Q000235:genetics / Q000276:immunology; D030241:Diarrhea Virus 1, Bovine Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000009:adverse effects / Q000235:genetics / Q000276:immunology; D006168:Guinea Pigs; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000009:adverse effects / Q000235:genetics / Q000276:immunology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D014759:Viral Envelope Proteins / Q000009:adverse effects / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology
D004279:DNA, Viral; D006023:Glycoproteins; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D014759:Viral Envelope Proteins; D014765:Viral Vaccines