Effect of inactivation of ccpA and aerobic growth in Lactobacillus plantarum: A proteomic perspective.
Lactobacillus plantarum is a facultative heterofermentative lactic acid bacterium widely used in the production of most fermented food due to its ability to thrive in several environmental niches, including the human gut. In order to cope with different growth conditions, it has developed complex molecular response mechanisms, characterized by the induction of a large set of proteins mainly regulated by HrcA and CtsR repressors as well as by global regulators such as carbon catabolite control protein A (CcpA). In this study, the role of CcpA in the regulation of growth under anaerobiosis and aerobiosis, and the adaptation to aeration in L. plantarum WCFS1 were comprehensively investigated by differential proteomics. The inactivation of ccpA, in both growth conditions, significantly changed the expression level of 76 proteins, mainly associated with carbohydrate and energy metabolism, membrane transport, nucleotide metabolism, protein biosynthesis and folding. The role of CcpA as pleiotropic regulator was particularly evident at the shift from homolactic fermentation to mixed fermentation. Proteomic results also indicated that the mutant strain was more responsive to aerobic growth condition.
Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell'Alimentazione, CNR, Avellino, Italy.
Maria F
MF
Mazzeo
Giuseppina
G
Cacace
Angela
A
Peluso
Teresa
T
Zotta
Lidia
L
Muscariello
Valeria
V
Vastano
Eugenio
E
Parente
Rosa A
RA
Siciliano
D000332:Aerobiosis; D001426:Bacterial Proteins / Q000096:biosynthesis*; D057465:Catabolite Repression / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D040901:Proteomics; D014157:Transcription Factors / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D014157:Transcription Factors