Protective effect of ferulic acid and resveratrol against alloxan-induced diabetes in mice.
The present study was to investigate the effect of ferulic acid and resveratrol on alloxan-induced diabetic mice, through analysis of basic biochemical parameters, enzymic as well as non-enzymic activities, lipid peroxidation and immunohistochemical studies. Alloxan was administered as a single dose (75 mg/kg body weight) to induce diabetes in mice. A dose of ferulic acid (10 mg/kg body weight) and resveratrol (20 mg/kg body weight) were administrated orally, to the alloxan-induced diabetic mice. The levels of basic biochemical markers and lipid peroxidation were significantly (P<0.05) increased in alloxan-induced diabetic mice. The levels of antioxidants were significantly (P<0.05) decreased in liver, kidney and serum. Immunohistochemical studies in alloxan induced mice demonstrated a marked increase in the immunoreactivity of nuclear transcription factor (NF-κB). Treating the diabetic mice with doses of ferulic acid and resveratrol restored the changes in the above parameters analyzed. The present study, showed that ferulic acid and resveratrol exerted antioxidant as well as anti-diabetic effects, consequently alleviate liver, kidney and pancreas damage caused by alloxan-induced diabetes, probably through inhibition of the proinflammatory factor, NF-κB.
Department of Animal Health and Management, Science block, Alagappa University, Karaikudi 630 003, India. manikandanramar@yahoo.co.in
Manikandan
M
Ramar
Beulaja
B
Manikandan
Thiagarajan
T
Raman
Asokan
A
Priyadarsini
Subramanian
S
Palanisamy
Meiyalagan
M
Velayudam
Arumugam
A
Munusamy
Narayanan
N
Marimuthu Prabhu
Baskaralingam
B
Vaseeharan
D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003373:Coumaric Acids / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D018450:Disease Progression; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D000975:Antioxidants; D015415:Biomarkers; D003373:Coumaric Acids; D016328:NF-kappa B; D013267:Stilbenes; C004999:ferulic acid; D000077185:Resveratrol