Detection and characterisation of alloreactive T cells.
T cell alloreactivity is responsible for much of the morbidity and mortality associated with tissue transplantation and graft versus host disease. Immunoassays for ex vivo monitoring and quantitation of alloreactive T cells are being increasingly utilised to provide valuable information for individualised clinical management of transplant recipients. Here we describe detailed methodologies for both traditional and novel assays utilised for the detection, quantitation, and functional characterisation of alloreactive T cells and highlight the key advantages and disadvantages of each system.
Department of Microbiology and Immunology, University of Melbourne, Parkville, VIC, Australia. mandvi@unimelb.edu.au
Mandvi
M
Bharadwaj
Nicole A
NA
Mifsud
James
J
McCluskey
D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007118:Immunoassay; D014184:Transplantation, Homologous
D006680:HLA Antigens