Zebrafish embryo developmental toxicology assay.
A promising in vitro zebrafish developmental toxicology assay was generated to test compounds for their teratogenic potential. The assay's predictivity is approximately 87% in AB strain fish (Brannen KC et al., Birth Defects Res B Dev Reprod Toxicol 89:66-77, 2010). The procedure entails exposing dechorionated gastrulation-stage embryos to a range of compound concentrations for 5 days throughout embryonic and larva development. The larvae are evaluated for viability in order to identify an LC25 (the compound concentration in which 25% lethality is observed) and morphological anomalies using a numerical score system to identify the NOAEL (no observed adverse effect level). These values are used to calculate the teratogenic index (LC25/NOAEL ratio) of each compound. If the teratogenic index is equal to or greater than 10 then the compound is classified as a teratogen, and if the ratio is less than 10 then the compound is classified as a nonteratogen (Brannen KC et al., Birth Defects Res B Dev Reprod Toxicol 89:66-77, 2010).
Discovery Toxicology, Bristol-Myers Squibb Company, Princeton, NJ, USA. julie.panzica@bms.com
Julieta M
JM
Panzica-Kelly
Cindy X
CX
Zhang
Karen
K
Augustine-Rauch
D000818:Animals; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects*; D007814:Larva / Q000033:anatomy & histology / Q000187:drug effects; D012107:Research Design; D013723:Teratogens / Q000145:classification / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods*; D015027:Zebrafish / Q000002:abnormalities* / Q000196:embryology
D013723:Teratogens