[Current applications of high-throughput DNA sequencing technology in antibody drug research].
Since the publication of a high-throughput DNA sequencing technology based on PCR reaction was carried out in oil emulsions in 2005, high-throughput DNA sequencing platforms have been evolved to a robust technology in sequencing genomes and diverse DNA libraries. Antibody libraries with vast numbers of members currently serve as a foundation of discovering novel antibody drugs, and high-throughput DNA sequencing technology makes it possible to rapidly identify functional antibody variants with desired properties. Herein we present a review of current applications of high-throughput DNA sequencing technology in the analysis of antibody library diversity, sequencing of CDR3 regions, identification of potent antibodies based on sequence frequency, discovery of functional genes, and combination with various display technologies, so as to provide an alternative approach of discovery and development of antibody drugs.
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
Xin
X
Yu
Qi-Gang
QG
Liu
Ming-Rong
MR
Wang
D000818:Animals; D000916:Antibody Diversity / Q000235:genetics*; D001483:Base Sequence; D022801:Complementarity Determining Regions / Q000737:chemistry / Q000235:genetics*; D004247:DNA / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D055808:Drug Discovery / Q000379:methods*; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*
D022801:Complementarity Determining Regions; D018076:DNA, Complementary; D004247:DNA