Reaction-based two-photon probes for in vitro analysis and cellular imaging of monoamine oxidase activity.
Reaction-based fluorescent probes for monoamine oxidases A and B are developed based on a new two-photon absorbing compound and its precursor. The probes show turn-on fluorescence response to the enzymes owing to the two-photon absorbing compound produced by the enzymatic activity, as monitored by one- as well as two-photon microscopy for the first time.
Department of Chemistry, Center for Electro-Photo Behaviors in Advanced Molecular Systems, POSTECH, San 31, Hyoja-dong, Pohang, 790-784, Republic of Korea.
Dokyoung
D
Kim
Sunderraman
S
Sambasivan
Hyoseok
H
Nam
Ki Hean
KH
Kim
Jin Yong
JY
Kim
Taiha
T
Joo
Kyung-Ha
KH
Lee
Kyong-Tai
KT
Kim
Kyo Han
KH
Ahn
D015081:2-Naphthylamine / Q000031:analogs & derivatives* / Q000737:chemistry; D000181:Acrylonitrile / Q000031:analogs & derivatives* / Q000737:chemistry; D000818:Animals; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D008995:Monoamine Oxidase / Q000032:analysis / Q000378:metabolism*; D017785:Photons; D051381:Rats
D005456:Fluorescent Dyes; D015081:2-Naphthylamine; D008995:Monoamine Oxidase; D000181:Acrylonitrile