Continual Gram-negative bacterial challenge accelerates stroke onset in stroke-prone spontaneously hypertensive rats.
This study examined the effects of continual Gram-negative bacterial challenge on stroke onset. Stroke onset occurred significantly earlier in stroke-prone spontaneously hypertensive rats (SHRSP) injected with a bacterial cell suspension of Gram-negative rods or lipopolysaccharides (LPSs) than in uninjected controls. Paralysis of the hindlimb, piloerection, hypokinesis, and hyperkinesis were observed in LPS-injected SHRSP but not in uninjected controls during stroke onset. The serum levels of NOx, thiobarbituric acid reactive substance, and 8-hydroxydeoxyguanosine increased in LPS-injected SHRSP. These results suggest that continual Gram-negative bacterial challenge induces accelerated stroke onset in SHRSP, probably caused by oxidative stress responses derived from LPSs.
Department of Oral Health Sciences, Nihon University School of Dentistry, Chiyodaku, Tokyo, Japan. kawato@dent.nihon-u.ne.jp
Takayuki
T
Kawato
Hideki
H
Tanaka
Masaki
M
Tabuchi
Kana
K
Ooshima
Kumiko
K
Nakai
Yoshihisa
Y
Yamashita
Masao
M
Maeno
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000367:Age Factors; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure; D001921:Brain / Q000473:pathology; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D006090:Gram-Negative Bacteria / Q000472:pathogenicity*; D016905:Gram-Negative Bacterial Infections / Q000150:complications; D006973:Hypertension / Q000150:complications* / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity; D009589:Nitrogen Oxides / Q000097:blood; D051381:Rats; D011918:Rats, Inbred SHR; D020521:Stroke / Q000097:blood / Q000209:etiology* / Q000382:microbiology* / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D015415:Biomarkers; D008070:Lipopolysaccharides; D009589:Nitrogen Oxides; D017392:Thiobarbituric Acid Reactive Substances; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine