Induction of epithelial-mesenchymal transition with O-glycosylated oncofetal fibronectin.
Epithelial-mesenchymal transition (EMT) has been shown to play a key role in embryogenesis and cancer progression. We previously found that fibronectin (FN) carrying O-GalNAc at a specific site is selectively expressed in cancer and fetal cells/tissues, and termed oncofetal FN (onfFN). Here, we show that (i) a newly-established monoclonal antibody against FN lacking the O-GalNAc, termed normalFN (norFN), is useful for isolation of onfFN, (ii) onfFN, but not norFN, can induce EMT in human lung carcinoma cells, (iii) onfFN has a synergistic effect with transforming growth factor (TGF)β1 in EMT induction.
Division of Biomembrane Research, Pacific Northwest Research Institute, 720 Broadway, Seattle, WA 98122, USA.
Yao
Y
Ding
Kirill
K
Gelfenbeyn
Leonardo
L
Freire-de-Lima
Kazuko
K
Handa
Sen-itiroh
SI
Hakomori
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition / Q000276:immunology / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism*; D017127:Glycation End Products, Advanced; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D000093844:Glycated Proteins
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D017127:Glycation End Products, Advanced; D053773:Transforming Growth Factor beta1; C045099:glycated fibronectin; D000093844:Glycated Proteins; C121542:oncofetal fibronectin