Using Cross-links to Study Ribosomal Dynamics.
Abstract   After publications of 3-D models of a static ribosome and its large and small subunits, one of the next tasks is to recognize movable ribosomal elements responsible for mechanical shifts during protein synthesis. Statistic analysis of available cross-linking data allowed us to reveal three well separated groups of motions in the ribosome: I, mean magnitude of 10Å; II, most abundant, centered at 20Å and of wide dispersion, and III, sparsely populated, with large distances up to 95Å. The last group, III, comprises elements, like the L7/12-stalk and the L1- protuberance, that adopt different positions in crystallographic or electron micrographic structures, and neighboring hairpins 88 and 89, indicating mobility. We demonstrate that the cross- linking method can be applied to study ribosomal dynamics, including large-scale functional movements and, in particular, to estimate which structures participate in molecular switches.
a    V.A. Engelhardt Institute of Molecular Biology Russian Academy of Sciences   ,    119991   ,    Moscow   ,    Russia.
Valery I
VI
Ivanov
Jason A
JA
Mears