Effect of ADH II deficiency on the intracellular redox homeostasis in Zymomonas mobilis.
Mutant strain of the facultatively anaerobic, ethanol-producing bacterium Zymomonas mobilis, deficient in the Fe-containing alcohol dehydrogenase isoenzyme (ADH II), showed impaired homeostasis of the intracellular NAD(P)H during transition from anaerobic to aerobic conditions, and also in steady-state continuous cultures at various oxygen supplies. At the same time, ADH II deficiency in aerobically grown cells was accompanied by a threefold increase of catalase activity and by about 50% increase of hydrogen peroxide excretion. It is concluded that ADH II under aerobic conditions functions to maintain intracellular redox homeostasis and to protect the cells from endogenous hydrogen peroxide.
Institute of Microbiology and Biotechnology, University of Latvia, Kronvalda Boulevard 4, 1586 Riga, Latvia.
Nina
N
Galinina
Zane
Z
Lasa
Inese
I
Strazdina
Reinis
R
Rutkis
Uldis
U
Kalnenieks
D000426:Alcohol Dehydrogenase / Q000172:deficiency*; D006706:Homeostasis / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D013045:Species Specificity; D017355:Zymomonas / Q000145:classification / Q000502:physiology*
D009249:NADP; D006861:Hydrogen Peroxide; D000426:Alcohol Dehydrogenase; D010100:Oxygen