Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers.
Although the genetic variability of hepatitis B virus (HBV) in HBV-infected patients has been extensively studied, reports on genotypes, subtypes and mutations in the S region of HBV strains from Chinese blood donors are limited. In this study, 245 blood samples from HBsAg-positive blood donors were collected from five geographically diverse blood centers in China. The S region of HBV was amplified, and the HBV genotype and subtype were determined. The amino acid sequences of the S region were aligned, and mutations related to the failure of immunization and HBsAg detection were determined. Of the 245 samples, 228 (93 %) were genotyped successfully. We found that genotypes B, C, D and A accounted for 58.8 %, 21.9 %, 6.6 % and 3.95 % of the isolates, respectively. The distribution of HBV antigen subtypes was as follows: adw (67.6 %), adr (23.3 %) and ayw (8.7 %). Mutations were present in 39 (17.1 %) of 228 samples in the major hydrophilic region (MHR) of the S region. This study demonstrated that HBV genotype/subtype B/adw was the most frequent strain circulating in HBV-infected Chinese blood donors, followed by C/adr. The occurrence of MHR mutants in HBV-infected blood donors and the potential failure to detect some of them in collected units poses a threat to transfusion safety.
Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Hua Cai Road 26 Hao, Dong San Huan Road Er Duan, Chengdu 610052, Sichuang, China. 21615620@qq.com
Yu
Y
Liu
Jingxing
J
Wang
Yi
Y
Huang
Tonghan
T
Yang
Xiaoming
X
Guo
Julin
J
Li
Guoxin
G
Wen
Zhongqiao
Z
Yun
Peibin
P
Zeng
Miao
M
He
Min
M
Xu
Gui
G
Liu
Ling
L
Ke
David
D
Wright
Jing
J
Liu
Kenrad
K
Nelson
Hua
H
Shan
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D001782:Blood Donors; D002681:China / Q000453:epidemiology; D016021:Epidemiologic Studies; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000453:epidemiology* / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D055815:Young Adult
D006514:Hepatitis B Surface Antigens