[Influence of D2-receptor blockade on the nitrergic system activity of the nucleus accumbens].
In Sprague-Dawley rats, by means of in vivo microdialysis combined with HPLC analysis it was shown that the infusion into the n. accumbens of the D2-receptor antagonist raclopride (20, 100 microM) did not affect extracellular level ofcitrulline (an NO co-product) in this brain area. The intraaccumbal infusion of NMDA, an NMDA receptor agonist (100 microM) caused a rise of the extracellular citrulline level in this brain area. This rise was prevented by intraaccumbal infusion of 0.5 mM 7-nitroindazole, a neuronal NO-synthase inhibitor, and it was significantly reduced by the infusion of raclopride (20, 100 microM) into this brain area. The data obtained suggest that the D2-receptors of the n. accumbens are implicated in the regulation of neuronal NO-synthase activity induced by local NMDA receptor stimulation.
N B
NB
Saul'skaia
P V
PV
Sudorgina
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002956:Citrulline / Q000096:biosynthesis* / Q000187:drug effects; D065127:Dopamine D2 Receptor Antagonists; D007191:Indazoles / Q000008:administration & dosage; D016202:N-Methylaspartate / Q000008:administration & dosage; D009474:Neurons / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009714:Nucleus Accumbens / Q000378:metabolism*; D020891:Raclopride / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists / Q000187:drug effects; D015398:Signal Transduction
D065127:Dopamine D2 Receptor Antagonists; D007191:Indazoles; D016194:Receptors, N-Methyl-D-Aspartate; D002956:Citrulline; D009569:Nitric Oxide; D020891:Raclopride; D016202:N-Methylaspartate; D019001:Nitric Oxide Synthase; C080122:7-nitroindazole