Effect of supplementation with an 80:20 cis9,trans11 conjugated linoleic acid blend on the human platelet proteome.
METHODS
The dietary fatty acid cis9,trans11 conjugated linoleic acid (cis9,trans11 CLA) has been shown to modify the function of endothelial cells, monocytes, and platelets, all of which are involved in the development of atherosclerosis. Potential mechanisms for the platelet effects have not been assessed previously. In this study, we assessed how supplementation of the diet with an 80:20 cis9,trans11 CLA blend affects the platelet proteome.


RESULTS
In a double-blind, randomized, placebo-controlled, parallel-group trial, 40 overweight but apparently healthy adults received either 4 g per day of cis9,trans11 CLA-enriched oil or placebo oil, consisting of palm oil and soybean oil, for 3 months. Total platelet proteins were extracted from washed platelets, separated using two-dimensional gel electrophoresis and differentially regulated protein spots were identified by LC-ESI-MS/MS. Supplementation with the CLA blend, compared with placebo, resulted in significant alterations in levels of 46 spots (p < 0.05), of which 40 were identified. Network analysis revealed that the majority of these proteins participate in regulation of the cytoskeleton and platelet structure, as well as receptor action, signaling, and focal adhesion.


CONCLUSIONS
The platelet proteomics approach revealed novel insights into regulation of cellular biomarkers of atherogenic and thrombotic pathways by an 80:20 cis9,trans11 CLA blend.
Rowett Institute of Nutrition & Health, University of Aberdeen, Bucksburn, Aberdeen, United Kingdom.
Eva-Maria
EM
Bachmair
Michiel L
ML
Bots
Louise I
LI
Mennen
Thomas
T
Kelder
Chris T
CT
Evelo
Graham W
GW
Horgan
Isobel
I
Ford
Baukje
B
de Roos
D050197:Atherosclerosis / Q000209:etiology / Q000517:prevention & control; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D015992:Body Mass Index; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism*; D019587:Dietary Supplements; D004311:Double-Blind Method; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D044243:Linoleic Acids, Conjugated / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D050177:Overweight / Q000097:blood / Q000178:diet therapy* / Q000378:metabolism / Q000503:physiopathology; D015398:Signal Transduction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013927:Thrombosis / Q000209:etiology / Q000517:prevention & control
D015415:Biomarkers; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D044243:Linoleic Acids, Conjugated; C503589:cis-9, trans-11-conjugated linoleic acid