A novel model of distal colon cancer in athymic mice.
OBJECTIVE
The present a novel adenocarcinoma model in athymic mice.


METHODS
Seven athymic mice were used. Colon diversion and distal fistula were made. Adenocarcinoma cells were inoculated in the submucosa of fistula. Tumor growth was monitored daily. Scintigraphy with 99mTc-MIBI was performed to identify the tumor.


RESULTS
The model of distal colon cancer is feasible. Tumor detection was possible by both, macroscopically and molecular imaging. All resections demonstrated poorly differentiated tumors. Colon obstruction occurred in one case, similarly to evolution in human tumors of distal colon.


CONCLUSIONS
The proposed model of distal colon cancer is feasible, allows for easy monitoring of tumoral growth by both, macroscopically and molecular imaging, and is suitable for studying the evolution of tumor with implementation of cytotoxic therapy in vivo.
Medical School, Sao Francisco University, Bragança Paulista, SP, Brazil. depriolli@terra.com.br
Denise Gonçalves
DG
Priolli
Ana Margarida
AM
Abrantes
Silvia
S
Neves
Joana Neves
JN
Batista
Izilda Aparecida
IA
Cardinalli
Maria Filomena
MF
Botelho
D000230:Adenocarcinoma / Q000000981:diagnostic imaging / Q000473:pathology; D000818:Animals; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D017256:Technetium Tc 99m Sestamibi; D014407:Tumor Cells, Cultured
D019275:Radiopharmaceuticals; D017256:Technetium Tc 99m Sestamibi