Molecularly imprinted polymer coated solid-phase microextraction fiber prepared by surface reversible addition-fragmentation chain transfer polymerization for monitoring of Sudan dyes in chilli tomato sauce and chilli pepper samples.
Surface reversible addition-fragmentation chain transfer (RAFT) polymerization method was firstly applied to the preparation of molecularly imprinted polymer (MIP) coated silicon solid-phase microextraction (SPME) fibers. With Sudan I as template, an ultra-thin MIP coating with about 0.55-μm thickness was obtained with homogeneous structure and controlled composition, due to the controllable radical growing and chain propagation in surface RAFT polymerization. The MIP-coated fibers were found with enhanced selectivity coefficients (3.0-6.5) to Sudan I-IV dyes in contrast with those reported in our previous work. Furthermore, the ultra-thin thickness of MIP coating was helpful to the effective elution of template and fast adsorption/desorption kinetics, so only about 18 min was needed for MIP-coated SPME operation. The detection limits of 21-55 ng L(-1) were achieved for four Sudan dyes, when MIP-coated SPME was coupled with liquid chromatography (LC) and mass spectrometry (MS) detection. The MIP-coated SPME-LC-MS/MS method was tested for the monitoring of ultra trace Sudan dyes in spiked chilli tomato sauce and chilli pepper samples, and high enrichment effect, remarkable matrix peaks-removing capability, and consequent high sensitivities were achieved to four Sudan dyes.
School of Chemistry and Environment, South China Normal University, Guangzhou 510631, PR China. huxg@scnu.edu.cn
Xiaogang
X
Hu
Yanan
Y
Fan
Yi
Y
Zhang
Guimei
G
Dai
Quanling
Q
Cai
Yujuan
Y
Cao
Changjuan
C
Guo
D002212:Capsicum / Q000737:chemistry*; D004396:Coloring Agents / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling; D057230:Limit of Detection; D016014:Linear Models; D018551:Solanum lycopersicum; D054802:Molecular Imprinting; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D052617:Solid Phase Microextraction; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
D004396:Coloring Agents; D011108:Polymers