Nano RNA aptamer wire for analysis of vitamin B₁₂.
A simple and stable RNA aptamer-based colorimetric sensor for the detection of vitamin B₁₂ using gold nanoparticles (AuNPs) has been proposed. Vitamin B₁₂ belongs to the B vitamin group and prevents pernicious anemia, which is caused by vitamin B₁₂ deficiency. A highly stable RNA aptamer that binds to vitamin B₁₂ was employed by structural modification of 2'-hydroxyl group of ribose to 2'-flouro in all pyrimidines indicated in lowercase in 35-mer aptamer (5' GGA Acc GGu GcG cAu AAc cAc cuc AGu GcG AGc AA 3'). Aggregation of AuNPs was specifically induced by desorption of the vitamin B₁₂ binding RNA aptamer from the surface of AuNPs as a result of the aptamer-target interaction, leading to the color change from red to purple. The level of detection of vitamin B₁₂ was 0.1 μg/ml by successful optimization of the amount of the aptamer, AuNPs, salts, and stability of the aptamer. Analysis of vitamin B₁₂ was carried out, and the observed recovery was 92 to 95.3% with a relative standard deviation in the range of 2.08 to 8.27%. The results obtained were compared with those of the ultraviolet-visible (UV-vis) spectrometry method. This colorimetric aptasensor is advantageous for on-site detection with the naked eye.
Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute-Constituent Laboratory of the Council of Scientific and Industrial Research, CSIR, Mysore 570020, India.
L S
LS
Selvakumar
M S
MS
Thakur
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001681:Biological Assay; D003124:Colorimetry; D006046:Gold / Q000737:chemistry*; D054879:Halogenation; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry*; D019999:Pharmaceutical Solutions / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013053:Spectrophotometry; D013607:Tablets / Q000737:chemistry; D014805:Vitamin B 12 / Q000032:analysis*
D052157:Aptamers, Nucleotide; D019999:Pharmaceutical Solutions; D011743:Pyrimidines; D013607:Tablets; D012965:Sodium Chloride; D006046:Gold; D014805:Vitamin B 12