[Regulation of BDNF expression in traumatic brain injury rats subjected single neucleated cell transplantation].
OBJECTIVE
To investigate the effect of single neucleated cells transplantation on the neurological function and brain derived neurotrophic factor (BDNF) expression in traumatic brain injury (TBI) rats.


METHODS
TBI rats were established by hammer fall method from 30 cm height with 50 g weight. Single neucleated cells/ karyocytes from bone marrow were separated and prepared by ficoll medium, then transplanted into pericontusional tissues in the brain. Neurological function severity scores (NSS) were recorded at 1, 3, and 7 days post operation (dpo). The pericontusional tissues were harvested at 7 dpo to analyze the BDNF localization, expressional level.


RESULTS
Single neucleated cell transplantation decreased NSS significantly, compared with TBI rats without cell implantation (P < 0.05). BDNF expression was upregulated and mainly found in neurons.


CONCLUSIONS
The present study showed the single karyocytes transplantation could improve neurological function and the mechanism is possibly linked to the BDNF expression.
Department of Neurosurgery, No. 1 Renming Hospital, Affiliated Kunming City, Kunming 650031, China.
Nan
N
Zhao
Qi-Qin
QQ
Dan
Jun
J
Liu
Gang
G
Ma
Jun-Yan
JY
Li
Hua
H
Jin
Yan-Bin
YB
Xiyang
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation / Q000379:methods; D001930:Brain Injuries / Q000378:metabolism / Q000628:therapy*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007963:Leukocytes, Mononuclear / Q000637:transplantation*; D008297:Male; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function
D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger