Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain.
Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as nanoCAGE and RNA seq.
Omics Science Center, RIKEN Yokohama Institute, 1-7-22, Suehiro Cho, 230-0045, Tsurumi, Yokohama 230-0045, Japan.
Alka
A
Saxena
Akiko
A
Wagatsuma
Yukihiko
Y
Noro
Takenobu
T
Kuji
Atsuko
A
Asaka-Oba
Akira
A
Watahiki
Cecile
C
Gurnot
Michela
M
Fagiolini
Takao K
TK
Hensch
Piero
P
Carninci
D000818:Animals; D001921:Brain / Q000166:cytology*; D002470:Cell Survival / Q000187:drug effects*; D005434:Flow Cytometry / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology*; D012333:RNA, Messenger / Q000302:isolation & purification; D059467:Transcriptome; D014199:Trehalose / Q000737:chemistry* / Q000494:pharmacology
D012333:RNA, Messenger; D014199:Trehalose