Multiview light-sheet microscope for rapid in toto imaging.
We present a multiview selective-plane illumination microscope (MuVi-SPIM), comprising two detection and illumination objective lenses, that allows rapid in toto fluorescence imaging of biological specimens with subcellular resolution. The fixed geometrical arrangement of the imaging branches enables multiview data fusion in real time. The high speed of MuVi-SPIM allows faithful tracking of nuclei and cell shape changes, which we demonstrate through in toto imaging of the embryonic development of Drosophila melanogaster.
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Uros
U
Krzic
Stefan
S
Gunther
Timothy E
TE
Saunders
Sebastian J
SJ
Streichan
Lars
L
Hufnagel
D000818:Animals; D004331:Drosophila melanogaster / Q000648:ultrastructure; D004625:Embryo, Nonmammalian / Q000648:ultrastructure; D047108:Embryonic Development; D004867:Equipment Design; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors