Peripheral blood lymphocytes from low-grade squamous intraepithelial lesions patients recognize vaccine antigens in the presence of activated dendritic cells, and produced high levels of CD8 + IFNγ + T cells and low levels of IL-2 when induced to proliferate.
BACKGROUND
Most infections with human papillomavirus (HPV) are resolved without clinical intervention, but a minority evolves into chronic lesions of distinct grades, including cervical-uterine cancer. It is known that in most cases the immune system mediates elimination of HPV infection. However, the mechanism of immune evasion leading to HPV persistence and development of early cervical lesions is not fully understood. The aim of the present work was to evaluate the potential of peripheral blood leukocytes (PBL) from low-grade squamous intraepithelial lesions (LSIL) patients to be activated ex-vivo by vaccine antigens, the participation of cytotoxic lymphocytes and regulatory T cells, and to determine the secretion of Th1 and Th2 cytokines mediated by stimulation of T cell receptors.


RESULTS
We found that PBL from LSIL patients showed a significantly lower proliferation rate to vaccine antigens as compared to that of healthy donors, even though there was not a difference in the presence of antibodies to those antigens in sera from both groups. We did not find differences in either the frequency of CD4 + CD25 + FoxP3+ in PBL, or the levels of IL-4, IL-5 and IL-10 in plasma or conditioned media from PBL incubated with TcR agonists in vitro, between the two groups. However, we detected a lower production of IL-2 and a higher proportion of CD8 + IFNγ + cells in PBL from LSIL patients as compared with PBL from normal donors. We also observed that PBL from patients infected by HPV-16 and -18 were not able to proliferate in the presence of soluble HPV antigens added to the culture; however, a high level of proliferation was attained when these antigens were presented by activated dendritic cells.


CONCLUSIONS
Our results suggest that the immunodeficiency reported in LSIL patients could be due to the inability of specific cytotoxic T lymphocytes that for some unknown reason are present but unable to mount a response when challenged with their antigens, probably related to an in situ IL-2 production deficiency.
Laboratorio de Inmunobiología, Unidad de Investigación en Diferenciación Celular y Cáncer, FES-Zaragoza, UNAM, México, Laboratorio 3, PB, UMIEZ, Campus II, Facultad de Estudios Superiores Zaragoza, UNAM, Batalla 5 de mayo s/n, Col, E, de Oriente, Esquina Fuerte de Loreto, Iztapalapa, CP 09230, México, DF, Mexico. mogl@servidor.unam.mx.
Jorge
J
Hernández-Montes
Leticia
L
Rocha-Zavaleta
Alberto
A
Monroy-García
Benny
B
Weiss-Steider
María Del Carmen
Mdel C
Zaragoza-Ortega
Fernando
F
Cruz-Talonia
Omar Cruz
OC
Y Cruz
Laura
L
Bonifaz-Alfonso
Adriana Karina
AK
Chávez-Rueda
Martha Patricia
MP
Rojo-Aguilar
María Victoria
MV
Legorreta-Haquet
María de Lourdes
Mde L
Mora-García