Antioxidant protection against curative and palliative doses of ionizing irradiation in human blood decreases with aging.
Reactive oxygen species (ROS) are independently recognized to play a significant role in radiation-induced damage on healthy tissue and in aging process. However, an age-related alteration of antioxidant (AO) system in radiation response in humans is poorly investigated. The aim of this paper was to evaluate the irradiation effects on the activities and expression of AO system in the blood of healthy women during aging. Blood samples were irradiated with curative and palliative doses of 2 Gy or 9 Gy γ-rays. AO capacity for detoxification of O(2)•(-) and H(2)O(2) in response to 2 Gy γ-irradiation decreases in women above 58 years, while in response to 9 Gy shows signs of weakening after 45 years of age. Due to reduction of AO capacity during aging, cytotoxic effects of curative and palliative doses of irradiation, mediated by ROS, may significantly increase in older subjects, while removal of H(2)O(2) excess could reduce them.
Laboratory of Molecular Biology and Endocrinology, Vinča Institute of Nuclear Sciences, University of Belgrade, Mike Petrovića Alasa 12-14, PO Box 522, 11 000 Belgrade, Serbia. jelkas@vinca.rs
Jelena
J
Kasapović
Vesna
V
Stojiljković
Ljubica
L
Gavrilović
Nataša
N
Popović
Zorka
Z
Milićević
D000367:Age Factors; D000368:Aged; D000375:Aging; D000975:Antioxidants / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008875:Middle Aged; D010100:Oxygen / Q000737:chemistry; D011839:Radiation, Ionizing; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D010100:Oxygen