Knockdown of radixin by RNA interference suppresses the growth of human pancreatic cancer cells in vitro and in vivo.
Radixin, encoded by a gene on chromosome 11, plays important roles in cell motility, invasion and tumor progression. However, its function in pancreatic cancer remains elusive. In this study, radixin gene expression was suppressed with a lentivirus-mediated short-hairpin RNA (shRNA) method. We found that radixin shRNA caused down-regulation of radixin in PANC-1 cells, associated with inhibition of pancreatic cancer cell proliferation, survival, adhesion and invasive potential in vitro. When radixin-silenced cells were implanted in nude mice, tumor growth and microvessel density were significantly inhibited as compared to blank control cells or nonsense shRNA control cells. Thrombospondin-1 (TSP-1) and E-cadherin were up-regulated in radixin-silenced PANC-1 cells. Our results suggest that radixin might play a critical role in pancreatic cancer progression, possibly through involvement of down-regulation of TSP-1 and E-cadherin expression.
Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Shu-Dong
SD
Chen
Mao-Min
MM
Song
Zhi-Qiang
ZQ
Zhong
Na
N
Li
Pi-Lin
PL
Wang
Shi
S
Cheng
Ri-Xing
RX
Bai
Hui-Sheng
HS
Yuan
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D015820:Cadherins / Q000096:biosynthesis; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering; D019700:Thrombospondin 1 / Q000096:biosynthesis
D015820:Cadherins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D034741:RNA, Small Interfering; D019700:Thrombospondin 1; C071776:radixin