Genetic variations in E-selectin and ICAM-1: relation to atherosclerosis.
BACKGROUND
This study aimed to investigate the association of both intercellular adhesion molecule-1 (ICAM-1) and endothelial cell adhesion molecule (E-selectin) polymorphisms using PCR technique and their role in the pathogenesis of atherosclerosis.


METHODS
The study enrolled 285 individuals, classified into 4 groups: 63 cerebrovascular atherosclerotic patients, 75 cardiovascular patients, 72 peripheral atherosclerotic patients and 75 normal healthy individuals.


RESULTS
The frequency of the mutant AC genotype of E-selectin in peripheral, cerebral and cardiovascular atherosclerotic patients was significantly higher than in control subjects (29.17%, 28.53% and 28% vs. 8%, respectively). However, no significant difference was observed in the frequency of mutant CC allele between all atherosclerotic patients and control groups. The frequency of the mutant EE homozygotes of ICAM-1 in peripheral, cerebral and cardiovascular atherosclerotic patients was significantly higher compared to controls (45.8%, 42.9% and 36% vs. 12%, respectively). The frequency of EK of ICAM-1 showed no significant difference between atherosclerotic patients and the control group. The frequency of the mutant E allele of ICAM-1 was significantly higher in peripheral, cerebral and cardiovascular patients compared to controls (58.3%, 54.8% and 54% vs. 26%, respectively).


CONCLUSIONS
Ser 128Arg of E-selectin and the K469E of ICAM-1 polymorphisms may be involved in predisposition to atherosclerosis.
Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Tarek
T
Motawi
Olfat
O
Shaker
Noha
N
Taha
Marwa
M
Abdel Raheem
D000328:Adult; D000368:Aged; D050197:Atherosclerosis / Q000097:blood / Q000235:genetics*; D001786:Blood Glucose / Q000378:metabolism; D003710:Demography; D019040:E-Selectin / Q000235:genetics*; D005215:Fasting / Q000097:blood; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D001786:Blood Glucose; D019040:E-Selectin; C573420:ICAM1 protein, human; D008055:Lipids; C546936:SELE protein, human; D018799:Intercellular Adhesion Molecule-1