Biodegradation of oil spill by petroleum refineries using consortia of novel bacterial strains.
Feasibility study carried out at the site prior to the full scale study showed that the introduced bacterial consortium effectively adapted to the local environment of the soil at bioremediation site. The soil samples were collected from the contaminated fields after treatment with bacterial consortium at different time intervals and analyzed by gas chromatography after extraction with hexane and toluene. At time zero (just before initiation of bioremediation), the concentration of total petroleum hydrocarbons in the soil (25-cm horizon) of plot A, B, C and D was 30.90 %, 18.80 %, 25.90 % and 29.90 % respectively, after 360 days of treatment with microbial consortia was reduced to 0.97 %, 1.0 %, 1.0 %, and 1.1 % respectively. Whereas, only 5 % degradation was observed in the control plot after 365 days (microbial consortium not applied).
Environmental and Industrial Biotechnology, The Energy Research Institute, Darbari Seth Block, Habitat Place Lodhi Road, New Delhi, 11003, India. binasingh28@gmail.com
Bina
B
Singh
Amit
A
Bhattacharya
Veeranna A
VA
Channashettar
C Paul
CP
Jeyaseelan
Sachin
S
Gupta
Priyangshu M
PM
Sarma
Ajoy K
AK
Mandal
Banwari
B
Lal
D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002849:Chromatography, Gas; D006586:Hexanes / Q000378:metabolism; D006838:Hydrocarbons / Q000378:metabolism; D059013:Microbial Consortia; D010578:Petroleum / Q000378:metabolism; D059392:Petroleum Pollution; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism
D006586:Hexanes; D006838:Hydrocarbons; D010578:Petroleum; D012989:Soil Pollutants; C026385:n-hexane; D014050:Toluene