Isolation of high quality RNA from embryonic kidney and cells.
All the mRNAs within a cell and their relative levels are indicative of gene expression within that cell, which is essential for its structure and function in its differentiated state. Therefore, methods for the identification of the specific mRNAs and the quantitation of their levels are invaluable tools for understanding gene expression. Due to high endogenous RNase activity within virtually all living cells, the isolation of good quality RNA with minimal degradation is not a trivial task. This protocol outlines a tried and tested methodology for isolating high quality RNA from embryonic kidneys for various applications including microarray analysis and quantitative reverse transcription PCR (qRT-PCR).
Department of Genetics and Development, Columbia University, New York, NY, USA.
Shifaan
S
Thowfeequ
Odyssé
O
Michos
D000818:Animals; D007668:Kidney / Q000196:embryology* / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013053:Spectrophotometry / Q000379:methods
D012313:RNA