Involvement of a putative cyclic amp receptor protein (CRP)-like binding sequence and a CRP-like protein in glucose-mediated catabolite repression of thn genes in Rhodococcus sp. strain TFB.
Glucose catabolite repression of tetralin catabolic genes in Rhodococcus sp. strain TFB was shown to be exerted by a protein homologous to transcriptional regulators of the cyclic AMP receptor (CRP)-FNR family. The protein was detected bound to putative CRP-like boxes localized at the promoters of the thnA1 and thnS genes.
Centro Andaluz de Biología del Desarrollo, CSIC-Universidad Pablo de Olavide-Junta de Andalucía, Seville, Spain.
Laura
L
Tomás-Gallardo
Eduardo
E
Santero
Belén
B
Floriano
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D057465:Catabolite Repression / Q000235:genetics / Q000502:physiology*; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D018274:Electroporation; D005947:Glucose / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012240:Rhodococcus / Q000378:metabolism / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013764:Tetrahydronaphthalenes / Q000378:metabolism*
D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein; D013764:Tetrahydronaphthalenes; D049452:Green Fluorescent Proteins; C095210:tetralin; D005947:Glucose