[The expressions and correlation of bcl-2 and Beclin-1 in pancreatic cancer].
OBJECTIVE
To investigate the expressions of Bcl-2 and Beclin-1 in pancreatic cancer and analyze the correlation between them.


METHODS
The pancreatic tissue samples were collected from each 6 cases of pancreatic cancer, pancreatic exocrine benign tumor, chronic pancreatitis and normal pancreas and marked as group A, group B, group C and group D, respectively. The mRNA expression levels of Bcl-2 and Beclin-1 were detected by real-time fluorescence quantitative PCR and the protein expression levels of Bcl-2 and Beclin-1 were detected through immunohistochemistry.


RESULTS
The expression levels of Bcl-2 mRNA and protein, were the lowest in group D and the highest in group A (P < 0.05). The expression levels of Beclin-1 mRNA and protein in group A were significantly lower than those in group B and group D (P < 0.05). However, the expression levels of Beclin-1 between group A and group C were not significantly different (P > 0.05). The correlation coefficient between Bcl-2 and Beclin-1 protein expression in pancreatic cancer is--0.827 (P = 0. 042).


CONCLUSIONS
Compared with normal pancreatic tissue, pancreatic cancer had Bcl-2 expression upregulated and Beclin-1 expression downregulated. The increased anti-apoptotic effect of Bcl-2 and the decreased autophagic effect of Beclin-1 may collaboratively contribute to the occurrence of pancreatic cancer.
Department of Hepatobiliopancreatic Surgery, West China Hospital, Sichuan University, Chengdu 400016, China.
Shuang-Shuang
SS
Cao
An-Ping
AP
Su
Xiao-Jiong
XJ
Du
Ang
A
Li
Wei-Minga
WM
Hu
Zhao-Da
ZD
Zhang
Bo-Le
BL
Tian
Hui-Min
HM
Lu
Neng-Wen
NW
Ke
Zhao-Peng
ZP
He
D000230:Adenocarcinoma / Q000378:metabolism*; D000328:Adult; D000368:Aged; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D000071186:Beclin-1; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D008565:Membrane Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger