Wheat TaRab7 GTPase is part of the signaling pathway in responses to stripe rust and abiotic stimuli.
Small GTP-binding proteins function as regulators of specific intercellular fundamental biological processes. In this study, a small GTP-binding protein Rab7 gene, designated as TaRab7, was identified and characterized from a cDNA library of wheat leaves infected with Puccinia striiformis f. sp. tritici (Pst) the wheat stripe rust pathogen. The gene was predicted to encode a protein of 206 amino acids, with a molecular mass of 23.13 KDa and an isoeletric point (pI) of 5.13. Further analysis revealed the presence of a conserved signature that is characteristic of Rab7, and phylogenetic analysis demonstrated that TaRab7 has the highest similarity to a small GTP binding protein gene (BdRab7-like) from Brachypodium distachyon. Quantitative real-time PCR assays revealed that the expression of TaRab7 was higher in the early stage of the incompatible interactions between wheat and Pst than in the compatible interaction, and the transcription level of TaRab7 was also highly induced by environmental stress stimuli. Furthermore, knocking down TaRab7 expression by virus induced gene silencing enhanced the susceptibility of wheat cv. Suwon 11 to an avirulent race CYR23. These results imply that TaRab7 plays an important role in the early stage of wheat-stripe rust fungus interaction and in stress tolerance.
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
Furong
F
Liu
Jun
J
Guo
Pengfei
P
Bai
Yinhui
Y
Duan
Xiaodong
X
Wang
Yulin
Y
Cheng
Hao
H
Feng
Lili
L
Huang
Zhensheng
Z
Kang
D001487:Basidiomycota / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004777:Environment; D018506:Gene Expression Regulation, Plant; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D015398:Signal Transduction / Q000502:physiology*; D013312:Stress, Physiological / Q000235:genetics*; D014908:Triticum / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000090802:rab7 GTP-Binding Proteins
D000090802:rab7 GTP-Binding Proteins; D020691:rab GTP-Binding Proteins