In vivo detoxification of furfural during lipid production by the oleaginous yeast Trichosporon fermentans.
In vivo detoxification of furfural by the oleaginous yeast, Trichosporon fermentans, under lipid-producing (i.e., nitrogen-limited) conditions was evaluated for the first time. During the initial fermentation phase, furfural was rapidly reduced to furfuryl alcohol, which is more toxic to T. fermentans than furfural. Furfuryl alcohol was subsequently oxidized to furoic acid which has low toxicity to T. fermentans and is the end product of the in vivo detoxification of furfural in this organism. These observations explain how T. fermentans can grow and accumulate lipids in medium containing furfural. They also indicate that strategies to minimize the transient production of furfuryl alcohol could further improve the capacity of the strain to produce lipids from furfural-containing lignocellulosic hydrolysates.
State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, People's Republic of China.
Chao
C
Huang
Hong
H
Wu
Thomas J
TJ
Smith
Zong-Jun
ZJ
Liu
Wen-Yong
WY
Lou
Min-Hua
MH
Zong
D001711:Biotransformation; D005662:Furaldehyde / Q000378:metabolism*; D005663:Furans / Q000378:metabolism; D050356:Lipid Metabolism; D053858:Metabolic Networks and Pathways; D010084:Oxidation-Reduction; D014250:Trichosporon / Q000378:metabolism*
D005663:Furans; C012986:furfuryl alcohol; D005662:Furaldehyde; C060089:2-furoic acid