Probe-based chemical modulations of tissues for IMS.
Chemical modulation imaging over a tissue is gaining momentum in the field of mass spectrometry. Some endogenous or exogenous compounds present in a tissue can be visualized by imaging mass spectrometry after chemical derivatization. This approach gives researchers the possibility to elude chemical interferences in components of the tissues, such as lipids or salts, as well as interferences caused by the matrix. The use of primary and secondary antibodies, the chemical derivatization of peptides and small molecules, and the use of (18)O labeling are various examples reviewed in this article to demonstrate the importance and potential of this emerging aspect of imaging mass spectrometry.
REQUIMTE, Departamento de Química, Centro de Química Fina e Biotecnologia (CQFB), Faculdade de Ciências e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), Campus de Caparica, 2829-516 Caparica, Portugal; Institute for Biotechnology and Bioengineering (IBB), Centre for Genomics and Biotechnology (CGB), University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal; Institute for Biotechnology and Biomedicine (IBB), University Autónoma of Barcelona (UAB), 08193 Bellaterra, Spain. Electronic address: hms14862@fct.unl.pt.
H M
HM
Santos
REQUIMTE, Departamento de Química, Centro de Química Fina e Biotecnologia (CQFB), Faculdade de Ciências e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), Campus de Caparica, 2829-516 Caparica, Portugal; Physical Chemistry Department, Science Faculty, University of Vigo at Ourense Campus, E-32004 Ourense, Spain.
J L
JL
Capelo
D000818:Animals; D055598:Chemical Phenomena; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008954:Models, Biological; D015335:Molecular Probes; D013048:Specimen Handling / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D015335:Molecular Probes