Appearance of WBC-platelet complex in acute ischemic stroke, predominantly in atherothrombotic infarction.
OBJECTIVE
Platelet aggregates with white blood cells (WBC-platelet complex) have recently been proposed as a marker of activated platelets, in addition to well-known molecular markers. We aimed to investigate the colocalization of activated platelets and WBC-platelet complex by means of flow cytometry, in patients with ischemic stroke.


METHODS
Eighty-six patients with cerebral infarction (CI) in the acute phase (58 males, 28 females; 65±14 years old) and 62 non-CI controls (23 males, 39 females; 53±14 years old) were registered. The appearance of WBC-platelet complex was quantified using 3-color flow cytometry.


RESULTS
The appearance rate of WBC-platelet complex was significantly higher in the CI group than in the controls. The appearance rate of WBC-platelet complex was significantly higher in atherothrombotic infarction (AT) than in lacunar infarction (LA) (p < 0.05). Furthermore, positive rates of both monocyte-platelet complex and granulocyte-platelet complex, but not lymphocyte-platelet complex, were significantly higher in the AT group than in the controls.


CONCLUSIONS
We concluded that WBC-platelet complex, especially involving monocytes and granulocytes, is a novel marker of platelet activation in the acute phase of ischemic stroke, mainly in AT.
Department of Neurology, Tokai University School of Medicine, Kanagawa, Japan. ishi-t@is.icc.u-tokai.ac.jp
Tatsuya
T
Ishikawa
Mie
M
Shimizu
Saori
S
Kohara
Shunya
S
Takizawa
Yasuhisa
Y
Kitagawa
Shigeharu
S
Takagi
D000208:Acute Disease; D000368:Aged; D001792:Blood Platelets / Q000473:pathology*; D002545:Brain Ischemia / Q000473:pathology*; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007962:Leukocytes / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation; D020521:Stroke / Q000473:pathology*