The influence of riverine barriers on phylogeographic patterns of Malagasy reed frogs (Heterixalus).
We analyzed the influence of large rivers on the phylogeography of endemic widespread amphibians along Madagascar's east coast, using as models various species and species complexes of Malagasy reed frogs (Heterixalus spp.) that are specialized to either highland or lowland habitats. We assembled a dense sampling across the full species ranges and used mitochondrial (cob) as well as nuclear (Rag-1) DNA sequences to assess their phylogeographies. A multigene mtDNA phylogeny of each species was constructed to establish the relationships among the main phylogroups, in order to understand the geographical regions of clade origins and possible directions of historical range expansions. Distinct intraspecific lineages as diagnosed by mitochondrial haplotype clades were found in highlands and lowlands. Most geographical boundaries among these phylogroups did not coincide with rivers, indicating that the influence of rivers on the primary divergence of phylogroups is probably minor in these frogs. Nevertheless, we found evidence for the influence of one riverine barrier in the lowland species complex, where the most important genetic discontinuity (the border between Heterixalus madagascariensis and H. alboguttatus) coincides with the geographical position of the Mangoro River on Madagascar's central east coast. Analyses of the highland species H. betsileo revealed the existence of six deep haplotype lineages, separated in two major subpopulations which differ largely in altitudinal distribution and do not overlap with the geographical settings of rivers in the highlands. Furthermore, our analyses indicated that most of the major intraspecific lineages of reed frogs show signs of a rather recent population expansion.
Division of Evolutionary Biology, Zoological Institute, Technical University of Braunschweig, Mendelssohnstr 4, 38106 Braunschweig, Germany.
Philip-Sebastian
PS
Gehring
Maciej
M
Pabijan
Jasmin E
JE
Randrianirina
Frank
F
Glaw
Miguel
M
Vences
D000818:Animals; D001001:Anura / Q000145:classification / Q000235:genetics*; D002467:Cell Nucleus / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D014644:Genetic Variation; D008270:Madagascar; D058974:Phylogeography; D045483:Rivers; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial