Stabilization of organophosphorus hydrolase by entrapment in silk fibroin: formation of a robust enzymatic material suitable for surface coatings.
Organophosphates are some of the most acutely toxic compounds synthesized on an industrial scale, and organophosphorus hydrolase (OPH) has the ability to hydrolyze and inactivate a number of these chemicals. However, OPH activity is vulnerable to harsh environmental conditions that would accompany its practical utility in the field; a limitation that can also be extended to conditions required for incorporation of OPH into useful materials. Here we present evidence that entrapment of OPH in silk fibroin leads to stabilization of OPH activity under a variety of conditions that would otherwise reduce free enzyme activity, such as elevated temperature, UV light exposure and the presence of detergent. Silk fibroin entrapment of OPH also allowed for its dispersal into a polyurethane-based coating that retained organophosphate hydrolysis activity after formulation, application and drying. Together, the data presented here demonstrate the utility of silk fibroin entrapment for the protection of OPH activity under a variety of environmental conditions.
Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson AFB, Ohio 45433, United States.
Patrick B
PB
Dennis
Anne Y
AY
Walker
Matthew B
MB
Dickerson
David L
DL
Kaplan
Rajesh R
RR
Naik
D000818:Animals; D043303:Aryldialkylphosphatase / Q000737:chemistry*; D012831:Bombyx; D004795:Enzyme Stability; D005349:Fibroins / Q000737:chemistry*; D006868:Hydrolysis; D007306:Insecticides / Q000737:chemistry; D008743:Methyl Parathion / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*; D011309:Preservation, Biological; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D014466:Ultraviolet Rays
D007306:Insecticides; D011140:Polyurethanes; D012967:Sodium Dodecyl Sulfate; D008743:Methyl Parathion; D005349:Fibroins; D043303:Aryldialkylphosphatase