A simple microscopy assay to teach the processes of phagocytosis and exocytosis.
Phagocytosis and exocytosis are two cellular processes involving membrane dynamics. While it is easy to understand the purpose of these processes, it can be extremely difficult for students to comprehend the actual mechanisms. As membrane dynamics play a significant role in many cellular processes ranging from cell signaling to cell division to organelle renewal and maintenance, we felt that we needed to do a better job of teaching these types of processes. Thus, we developed a classroom-based protocol to simultaneously study phagocytosis and exocytosis in Tetrahymena pyriformis. In this paper, we present our results demonstrating that our undergraduate classroom experiment delivers results comparable with those acquired in a professional research laboratory. In addition, students performing the experiment do learn the mechanisms of phagocytosis and exocytosis. Finally, we demonstrate a mathematical exercise to help the students apply their data to the cell. Ultimately, this assay sets the stage for future inquiry-based experiments, in which the students develop their own experimental questions and delve deeper into the mechanisms of phagocytosis and exocytosis.
Department of Biology, University of Central Arkansas, Conway, AR 72034, USA.
Ross
R
Gray
Andrew
A
Gray
Jessica L
JL
Fite
Renée
R
Jordan
Sarah
S
Stark
Kari
K
Naylor
D008841:Actin Cytoskeleton / Q000378:metabolism; D001681:Biological Assay / Q000379:methods*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D003585:Cell Biology / Q000193:education*; D002462:Cell Membrane / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology; D022162:Cytoplasmic Vesicles / Q000187:drug effects / Q000378:metabolism; D004521:Educational Measurement; D005089:Exocytosis; D008853:Microscopy / Q000379:methods*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D010587:Phagocytosis; D013663:Teaching; D013768:Tetrahymena / Q000166:cytology; D053778:Thiazolidines / Q000494:pharmacology
D019086:Bridged Bicyclo Compounds, Heterocyclic; D053778:Thiazolidines; D003078:Colchicine; C037067:latrunculin A