Effect of ring-substituted oxysterols on the phase behavior of dipalmitoylphosphatidylcholine membranes.
Oxysterols are oxygenated derivatives of cholesterol that form a class of potent regulatory molecules with diverse biological activity. Given the implications of oxysterols in several physiological/pathophysiological pathways of human diseases, it is important to identify how their presence affects the biophysical properties of cell membranes. In this article we first describe the structure, formation, and biological functions of oxysterols, and previous work on the effect of these molecules on the structure and phase behavior of lipid membranes. We then present results of our X-ray diffraction experiments on aligned multilayers of dipalmitoylphosphatidylcholine (DPPC) membranes containing ring-substituted oxysterols. The effect of these molecules on the phase behavior of DPPC membranes is found to be very similar to that of cholesterol. All the oxysterols studied induce a modulated phase in DPPC membranes, similar to that reported in DPPC-cholesterol membranes. However, some differences are observed in the ability of these molecules to suppress the main transition of the lipid and to induce chain ordering, which might be related to differences in their orientation in the bilayer.
Raman Research Institute, C V Raman Avenue, Sadashivanagar, Bangalore, 560 080, India. kmal@rri.res.in
Md Arif
MA
Kamal
V A
VA
Raghunathan
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002784:Cholesterol / Q000031:analogs & derivatives / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D014961:X-Ray Diffraction
D008051:Lipid Bilayers; D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol