[Effects of dietary fat level on the xenobiotic metabolism enzymes activity and antioxidant enzymes in rats].
Male Wistar rats received fat-free diet or diets containing 5, 10 and 30% of fat (sunflower oil + lard, 1:1) for 4 weeks. The direct relationship between dietary fat level and ethoxyresorufin O-dealkylase activity of CYP1A1, methoxyresorufin O-dealkylase activity of CYP1A2, pentoxyresorufin O-dealkylase activity of CYP2B1 and testosterone 6beta-hydroxylase activity of CYP3A was found. Activities of key enzymes of phase II xenobiotic metabolism (total activity of glutathione transferase, activity of UDP-glucuronosyle transferase) and antioxidant enzymes (catalase, glutathione peroxidase, glutathione reductase, paraoxonase-1 and heme oxygenase-1) also increased with higher dietary fat level.
L V
LV
Kravchenko
I V
IV
Aksenov
N V
NV
Trusov
G V
GV
Guseva
L I
LI
Avren'eva
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis / Q000378:metabolism*; D043303:Aryldialkylphosphatase / Q000097:blood / Q000378:metabolism; D002374:Catalase / Q000032:analysis / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis / Q000378:metabolism; D019388:Cytochrome P-450 CYP1A2 / Q000032:analysis / Q000378:metabolism; D019362:Cytochrome P-450 CYP2B1 / Q000032:analysis / Q000378:metabolism; D051544:Cytochrome P-450 CYP3A / Q000032:analysis / Q000378:metabolism; D018752:Diet, Fat-Restricted; D004041:Dietary Fats / Q000008:administration & dosage* / Q000032:analysis / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000032:analysis / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000032:analysis / Q000378:metabolism; D005980:Glutathione Reductase / Q000032:analysis / Q000378:metabolism; D005982:Glutathione Transferase / Q000032:analysis / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D008862:Microsomes, Liver / Q000201:enzymology*; D010938:Plant Oils / Q000008:administration & dosage / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000074242:Sunflower Oil
D000975:Antioxidants; D004041:Dietary Fats; D010938:Plant Oils; D000074242:Sunflower Oil; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D001189:Aryl Hydrocarbon Hydroxylases; D019363:Cytochrome P-450 CYP1A1; D019388:Cytochrome P-450 CYP1A2; D019362:Cytochrome P-450 CYP2B1; D051544:Cytochrome P-450 CYP3A; D051547:Heme Oxygenase-1; D005980:Glutathione Reductase; D014453:Glucuronosyltransferase; C107611:UDP-glucuronosyltransferase, UGT1A7; D005982:Glutathione Transferase; D043303:Aryldialkylphosphatase; C029310:lard