Preparation and characterization of DNA aptamer based spin column for enrichment and separation of histones.
Core histones play a critical role in the regulation of chromatin-templated biological process. Here we have developed a novel approach to enrich histone proteins based on the recognition of a specific aptamer. The method shows good selectivity toward histone proteins and is able to extract histones directly from complex whole cell lysates.
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, P. R. China.
Nan
N
Shao
Kai
K
Zhang
Ying
Y
Chen
Xiwen
X
He
Yukui
Y
Zhang
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006657:Histones / Q000032:analysis / Q000737:chemistry*; D006801:Humans
D052157:Aptamers, Nucleotide; D006657:Histones