Polymeric fibrous matrices for substrate-mediated human embryonic stem cell lineage differentiation.
Chitosan-based fibrous matrices are prepared to mimic the ECM architecture and elucidate substrate-mediated hESC differentiation due to topographical scale and anisotropy without exogenic morphogens. Fibrous matrices support fewer pluripotent hESCs than films but enable topography-mediated hESC differentiation. Matrices composed of 400 nm and 1.1 µm diameter fibers support increased expression of neural markers indicative of ectodermal commitment while matrices of 200 nm diameter fibers increase expression of osteogenic and hepatic markers indicative of endodermal and mesodermal commitment. The fibrous-mediated hESC differentiation highlights the significant implication of tailored ECM-like substrates for hESC-based therapies.
Department of Materials Science & Engineering, University of Washington, Seattle, WA 98195, USA.
Ashleigh
A
Cooper
Matthew
M
Leung
Miqin
M
Zhang
D015415:Biomarkers / Q000378:metabolism; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D019070:Cell Lineage; D048271:Chitosan / Q000737:chemistry*; D004475:Ectoderm; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects*; D004707:Endoderm; D005109:Extracellular Matrix; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008855:Microscopy, Electron, Scanning; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects*; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering
D015415:Biomarkers; D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan