Combination of acupuncture and chinese medicinal herbs in treating model rats with polycystic ovary syndrome.
We explored the effects of combination of acupuncture and Chinese medicinal herbs in treating model rats with polycystic ovarian syndrome (PCOS) and to explore whether acupuncture has positive effects on the absorption of salvianolic acid B in the extracts of a Chinese medicine formula when treating the model rats. 60 female Sprague-Dawley (SD) rats were randomly divided into Groups A, B, C, D, E and F, with ten rats in each group. Except Group F, all of the other rats were induced to PCOS with oral administration of letrozole. The rats in Group F served as normal controls. Group A was treated with acupuncture. Group B was treated with oral administration of the extracts of the Chinese medicine formula. Group C was treated with a combination of oral administration of the extracts of Chinese medicine and acupuncture. Group D received western medicine as positive controls. After treatment, the serum levels of follicle stimulating hormone (FSH), luteinizing hormone(LH) and testosterone (T) in each group were detected with the Enzyme-Linked ImmunoSorbent Assay (ELISA) and the serum concentration of salvianolic acid B were determined using High Performance Liquid Chromatography (HPLC). The serum levels of T and the ratio of LH/FSH in Group A, B. C, D, and F were significantly lower than those of Group E, indicating the model rats with PCOS were successfully established. Compared with Groups A, B, D and E, the serum levels of T and the ratio of LH/FSH in Group C were significantly lower respectively, indicating combination of acupuncture and Chinese medicinal herbs can significantly enhance curative effects in treating model rats with PCOS. The concentration of serum salvianolic acid Group C was significantly higher than Group B, indicating that acupuncture might improve the absorption of salvianolic acid B from the extracts of Salvia miltiorrhiza Bunge in the Chinese medicine formula. Combination of acupuncture and Chinese medicinal herbs significantly enhance curative effects in treating model rats with PCOS and acupuncture has positive effects in improving the absorption of salvianolic acid B in the extracts of the Chinese medicine formula when treating the model rats with PCOS.
The third clinical college of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
Rui-Jie
RJ
Ma
Jue
J
Zhou
Jian-Qiao
JQ
Fang
Dan-Hong
DH
Yang
Fan
F
Qu
D015670:Acupuncture Therapy; D000284:Administration, Oral; D000818:Animals; D001572:Benzofurans / Q000097:blood / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D000077289:Letrozole; D007986:Luteinizing Hormone / Q000097:blood; D009570:Nitriles; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000139:chemically induced / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000097:blood; D016896:Treatment Outcome; D014230:Triazoles; D053630:Depsides
D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D005640:Follicle Stimulating Hormone; D000077289:Letrozole; D007986:Luteinizing Hormone; D009570:Nitriles; D013739:Testosterone; D014230:Triazoles; C076944:salvianolic acid B; D053630:Depsides