Detection of vanA-containing Enterococcus species in faecal microbiota of gilthead seabream (Sparus aurata).
Vancomycin-resistant Enterococcus faecalis, E. faecium and E. durans isolates with the genotype vanA were detected in 7 of 118 faecal samples (5.9%) of natural gilthead seabream recovered off the coast of Portugal, and one vancomycin-resistant isolate/sample was further characterized. The genes erm(B), tet(L), tet(M), aac(6')-aph(2"), aph(3')-IIIa and/or ant(6)-Ia were identified in most of the 7 vancomycin-resistant enterococci. Sequence types ST273, ST313 and ST76 were detected in three E. faecium isolates and ST6 in two E. faecalis isolates. VanA-containing enterococci are suggested to be disseminated in fish in marine ecosystems close to areas of human activity.
Center of Genetics and Biotechnology, University of Tras-os-Montes and Alto Douro, Vila Real, Portugal.
Joana
J
Barros
Margarida
M
Andrade
Hajer
H
Radhouani
Maria
M
López
Gilberto
G
Igrejas
Patricia
P
Poeta
Carmen
C
Torres
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D017753:Ecosystem; D016983:Enterococcus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D016984:Enterococcus faecium / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D011174:Portugal; D021541:Sea Bream / Q000382:microbiology*; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; D014640:Vancomycin; D019729:Carbon-Oxygen Ligases