Enrichment of neural-related genes in human mesenchymal stem cells from             neuroblastoma patients.
Neuroblastoma (NB) is one of the most common pediatric solid tumors and,             like most human cancers, is characterized by a broad variety of genomic alterations.             Although mesenchymal stem cells (MSCs) are known to interact with cancer cells,             the relationship between MSCs and metastatic NB cancer cells in bone marrow (BM)             is unknown. To obtain genetic evidence about this interaction, we isolated ΒΜ-derived             MSCs from children with NB and compared their global expression patterns with             MSCs obtained from normal pediatric donors, using the Agilent 44K microarrays.             Significance analysis of microarray results with a false discovery rate (FDR) <5%             identified 496 differentially expressed genes showing either a 2-fold upregulation             or downregulation between both groups of samples. Comparison of gene ontology             categories of differentially expressed genes revealed the upregulation of genes             categorized as 'neurological system process', 'cell adhesion', 'apoptosis', 'cell             surface receptor linked signal transduction', 'intrinsic to membrane' and 'extracellular             region'. Among the downregulated genes, several immunology-related terms were             the most abundant. These findings provide preliminary genetic evidence of the             interaction between MSCs and NB cancer cells in ΒΜ as well as identify relevant             biological processes potentially altered in MSCs in response to NB.
Cellular Biotechnology Unit, Institute of Health Carlos III,                     Majadahonda, Madrid, Spain.
Miguel Angel
MA
Rodriguez-Milla
Isabel
I
Mirones
Luis
L
Mariñas-Pardo
Gustavo J
GJ
Melen
Isabel
I
Cubillo
Manuel
M
Ramírez
Javier
J
García-Castro
D001854:Bone Marrow Cells / Q000378:metabolism; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism*