[Establishment of a cisplatin-resistant human cervical cancer cell line].
OBJECTIVE
To establish a cisplatin (cDDP)-resistant human cervical cancer cell line named SiHa/cDDP and researched its biological characteristics.


METHODS
The development of cDDP resistance in SiHa cell line was induced by continuously stepwise exposure of the cells to cDDP. Cell growth curve, doubling time and resistance index (RI) were evaluated by MTT analysis. The expression of P-gp, GST-pi, Topo I were assessed with immunocytochemical method.


RESULTS
SiHa/cDDP cell line was successfully established which had stable growth, subculture, frozen reservation and resuscitation in the concentration of 2 microg/ml cDDP, doubling time was (45.82 +/- 3.69) h and RI to cDDP was 16.131. It also showed different degrees of cross-resistance to several anticancer drugs. As compared to parental SiHa, SiHa/cDDP had over-expression in P-gp and GST-pi (P < 0.01), but Topo I showed no statistically significant differences.


CONCLUSIONS
We successfully established the cDDP-resistant human cervical cancer cell line SiHa/cDDP, which may provide ideal experimental model for research of human cervical carcinoma.
Laboratory of Genetics, West China Institute of Women and Children's Health, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Xin-Lian
XL
Chen
He
H
Wang
Xue-Mei
XM
Zhang
Hong-Qian
HQ
Liu
Shan-Ling
SL
Liu
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D019008:Drug Resistance, Neoplasm; D005260:Female; D051549:Glutathione S-Transferase pi / Q000378:metabolism; D006801:Humans; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
D018435:ATP Binding Cassette Transporter, Subfamily B; D000970:Antineoplastic Agents; D051549:Glutathione S-Transferase pi; D004250:DNA Topoisomerases, Type II; D002945:Cisplatin