KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry.
Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair, dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.
Department of Chemistry, Universität Konstanz, Konstanz, Germany.
Karin
K
Betz
Denis A
DA
Malyshev
Thomas
T
Lavergne
Wolfram
W
Welte
Kay
K
Diederichs
Tammy J
TJ
Dwyer
Phillip
P
Ordoukhanian
Floyd E
FE
Romesberg
Andreas
A
Marx
D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D019914:Taq Polymerase / Q000378:metabolism*
D004247:DNA; D019914:Taq Polymerase