Disruption of the ECM33 gene in Candida albicans prevents biofilm formation, engineered human oral mucosa tissue damage and gingival cell necrosis/apoptosis.
In this study we demonstrated that ΔCaecm33 double mutant showed reduced biofilm formation and causes less damage to gingival mucosa tissues. This was confirmed by the reduced level of necrotic cells and Bax/Bcl2 gene expression as apoptotic markers. In contrast, parental and Caecm33 mutant strains decreased basement membrane protein production (laminin 5 and type IV collagen). We thus propose that ECM33 gene/protein represents a novel target for the prevention and treatment of infections caused by Candida.
Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval Pavillon de Médecine Dentaire, 2420 Rue de la Terrasse, QC G1V 0A6, Canada.
Mahmoud
M
Rouabhia
Abdelhabib
A
Semlali
Jyotsna
J
Chandra
Pranab
P
Mukherjee
Witold
W
Chmielewski
Mahmoud A
MA
Ghannoum
D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D002176:Candida albicans / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D002177:Candidiasis / Q000517:prevention & control; D002478:Cells, Cultured; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D009061:Mouth Mucosa / Q000382:microbiology*; D009336:Necrosis / Q000517:prevention & control
D005656:Fungal Proteins