Biosurfactants prevent in vitro Candida albicans biofilm formation on resins and silicon materials for prosthetic devices.
OBJECTIVE
The aim of this study was to evaluate in vitro the preventive antiadhesion activity of biosurfactants against Candida albicans biofilm.


METHODS
Disks of silicon and acrylic resin for denture prostheses were precoated with increasing concentrations of biosurfactants obtained from endophyte biofilms selected from Robinia pseudoacacia and from Nerium oleander, and afterward infected with C. albicans cells. The number of biofilm cells were detected by colony-forming unit (CFU) counting, cell viability was established by the 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenyl amino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay, and biosurfactant cytotoxicity was evaluated by the [3-(4,5-dimethyliazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium] (MTT) assay. Chlorhexidine was used as control.


RESULTS
Precoating with biosurfactants caused a greater reduction (P < .01) in biofilm cell number and viability than chlorhexidine. The antiadhesion activity of the biosurfactants was observed at low concentrations (78.12 μg/mL and 156.12 μg/mL) which were noncytotoxic.


CONCLUSIONS
This study demonstrated the preventive antiadhesion activity of biosurfactants against C. albicans biofilm. These agents are amphiphilic, interfere with microbial adhesion, and demonstrate cycompatibility with epithelial cells and fibroblasts.
Dipartimento di Scienze della Salute, Amedeo Avogadro Università del Piemonte Orientale, Novara, Italy.
Andrea
A
Cochis
Letizia
L
Fracchia
Maria Giovanna
MG
Martinotti
Lia
L
Rimondini
D000180:Acrylic Resins; D000818:Animals; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D015169:Colony Count, Microbial; D017266:Dental Prosthesis / Q000382:microbiology*; D004129:Dimethylpolysiloxanes; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D008422:Materials Testing; D051379:Mice; D009010:Monte Carlo Method; D030007:Nerium; D028321:Plant Preparations / Q000494:pharmacology*; D031301:Robinia; D018709:Statistics, Nonparametric; D013501:Surface-Active Agents / Q000494:pharmacology* / Q000633:toxicity
D000180:Acrylic Resins; D004129:Dimethylpolysiloxanes; D028321:Plant Preparations; D013501:Surface-Active Agents; C024149:paladon