[Constrution of human single herpes virus carried BDNF gene vector and its bioactivity evaluation].
OBJECTIVE
To construct the recombinant vector of human single herpes virus (HSV) carried brain derived neurotrophic factor (BDNF) gene.


METHODS
BDNF gene was acquired from rat brain tissue by RT-PCR, then was cloned into plasmid, and enveloped by HSV. The recombinant was used to transfer cultured cortical neurons. The number and neurite length of neurons were quantified. The BDNF level and subcellular localization were detected by ELISA and immunohistochemistry.


RESULTS
HSV carried BDNF gene recombinant has been successfully constructed. The recombinant showed the bioactivity on the growth of cortical neurons. BDNF level was increased significantly in BDNF transferred group.


CONCLUSIONS
HSV carried BDNF gene recombinant, with the bioactivity, has been successfully constructed. This could provide the vector for the treatment of BDNF under disease condition base on transferring gene technique.
Department of Resparation, the First Affiliated Hospital of Yunnan Province, Kunhua Hospital, Kunming Medical College, Kunming 650031, China.
Sheng-Lan
SL
Wang
Qi-Qin
QQ
Dan
Rong
R
Rong
Ting-Hua
TH
Wang
Yun-Hui
YH
Zhang
D000818:Animals; D001483:Base Sequence; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D003001:Cloning, Molecular; D005822:Genetic Vectors; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D019208:Brain-Derived Neurotrophic Factor; D011994:Recombinant Proteins