Study of saiga horn using high-performance liquid chromatography with mass spectrometry.
The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are keratins and collagen. The basic representation protein in all samples is keratin type I microfibrillar (from sheep), keratin type II microfibrillar (from sheep), collagen type I (α(1)) (from bovine) and collagen type I (α(2)) (from bovine). Free amino acids we determined in all samples are nontreated by enzyme.
Institute of Physiology, Academy of Science of the Czech Republic, VÍdeňská 1083, Prague, Czech Republic.
Kateřina
K
Mikulíková
Oleg
O
Romanov
Ivan
I
Miksik
Adam
A
Eckhardt
Statis
S
Pataridis
Pavla
P
Sedláková
D000818:Animals; D000866:Antelopes / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006733:Horns / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*