An ascorbic acid amperometric sensor using over-oxidized polypyrrole and palladium nanoparticles composites.
We constructed a highly responsive ascorbic acid (AA) sensor utilizing over-oxidized polypyrrole (OPPy) and Palladium nanoparticles (PdNPs) composites (OPPy-PdNPs). In the presence of PdNPs, polypyrrole (PPy) was coated on a gold (Au) electrode through cyclic voltammetry (CV) and over-oxidized at a fixed potential in NaOH solution. The PdNPs were characterized using ultraviolet-visible (UV-vis) spectrum and transmission electron microscopy (TEM). The surface of OPPy-PdNPs on the Au electrode was investigated using field-emission scanning electron microscopy (FE-SEM). Results revealed that the OPPy-PdNPs-modified Au electrode (OPPy-PdNPs/Au) has the capacity to catalyze the oxidation of AA by lowering its oxidation potential to 0 V. The OPPy-PdNPs/Au electrode exhibited 2 different linear concentration ranges. In the low concentration range (1-520 μM), OPPy-PdNPs/Au exhibited a direct linear relation with current responses and had high sensitivity (570 μA mM(-1)cm(-2)) and a high correlation coefficient (0.995). In contrast, in the higher concentration range (120-1600 μM), the relationship between current responses and concentration of AA can be represented by a two-parameter sigmoidal equation. In addition, the sensor exhibited a short response time (less than 2s) and a very low limit of detection of 1 μM. The electrochemical AA sensor constructed in this study was simple, inexpensive, reproducible, sensitive, and resistant to interference. Thus, the proposed sensor has great potential for detecting AA in complex biosystems and can be applied in various fields, particularly neuroscience.
State Key Laboratory of Transducer, Institute of Electronics, Chinese Academy of Science, Beijing 100190, PR China.
Wentao
W
Shi
Chunxiu
C
Liu
Yilin
Y
Song
Nansen
N
Lin
Shuai
S
Zhou
Xinxia
X
Cai
D001205:Ascorbic Acid / Q000032:analysis*; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods; D055664:Electrochemical Techniques / Q000191:economics / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010165:Palladium / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D010165:Palladium; D006046:Gold; D001205:Ascorbic Acid