Isolation and characterisation of a human-like antibody fragment (scFv) that inactivates VEEV in vitro and in vivo.
Venezuelan equine encephalitis virus (VEEV) belongs to the Alphavirus genus and several species of this family are pathogenic to humans. The viruses are classified as potential agents of biological warfare and terrorism and sensitive detection as well as effective prophylaxis and antiviral therapies are required.In this work, we describe the isolation of the anti-VEEV single chain Fragment variable (scFv), ToR67-3B4, from a non-human primate (NHP) antibody gene library. We report its recloning into the bivalent scFv-Fc format and further immunological and biochemical characterisation.The scFv-Fc ToR67-3B4 recognised viable as well as formalin and ß-propionolactone (ß-Pl) inactivated virus particles and could be applied for immunoblot analysis of VEEV proteins and immuno-histochemistry of VEEV infected cells. It detected specifically the viral E1 envelope protein of VEEV but did not react with reduced viral glycoprotein preparations suggesting that recognition depends upon conformational epitopes. The recombinant antibody was able to detect multiple VEEV subtypes and displayed only marginal cross-reactivity to other Alphavirus species except for EEEV. In addition, the scFv-Fc fusion described here might be of therapeutic use since it successfully inactivated VEEV in a murine disease model. When the recombinant antibody was administered 6 hours post challenge, 80% to 100% of mice survived lethal VEEV IA/B or IE infection. Forty to sixty percent of mice survived when scFv-Fc ToR67-3B4 was applied 6 hours post challenge with VEEV subtypes II and former IIIA. In combination with E2-neutralising antibodies the NHP antibody isolated here could significantly improve passive protection as well as generic therapy of VEE.
Technische Universität Braunschweig, Institut für Biochemie und Biotechnologie, Braunschweig, Germany.
Torsten
T
Rülker
Luzie
L
Voß
Philippe
P
Thullier
Lyn M
LM
O' Brien
Thibaut
T
Pelat
Stuart D
SD
Perkins
Claudia
C
Langermann
Thomas
T
Schirrmann
Stefan
S
Dübel
Hans-Jürgen
HJ
Marschall
Michael
M
Hust
Birgit
B
Hülseweh
D000818:Animals; D057134:Antibodies, Neutralizing / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D003001:Cloning, Molecular; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology*; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007116:Immunization, Passive; D008252:Macaca fascicularis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D017421:Sequence Analysis; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies