Biochemical and molecular analysis of some commercial samples of chilli peppers from Mexico.
The genus Capsicum provides antioxidant compounds, such as phenolics and carotenoids, into the diet. In Mexico, there is a wide diversity of species and varieties of chilli peppers, a fruit which has local cultural and gastronomic importance. In the present study, the relationship of the carotenoid and phenolic profiles with the RAPD fingerprint of three different commercial cultivars of chilli peppers of seven regions of Mexico was investigated. Through RAPD, the species of chilli were differentiated by means of different primers (OPE-18, MFG-17, MFG-18, C51, and C52). The genetic distance found with OPE 18 was in the order of 2.6. The observed differences were maintained when the chromatographic profile of carotenoids, and the molecular markers were analyzed, which suggest a close relationship between carotenoids and the genetic profile. While the chromatographic profile of phenols and the molecular markers were unable to differentiate between genotypes of chilli peppers. In addition, by using infrared spectroscopy and statistical PCA, differences explained by geographic origin were found. Thus, this method could be an alternative for identification of chilli species with respect to their geographic origin.
Departamento de Ingenieria Bioquimica, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, Carpio y Plan de Ayala s/n Col, Casco de Santo Tomás, 11340 Mexico City, DF, Mexico.
Ivonne Guadalupe
IG
Troconis-Torres
Marlon
M
Rojas-López
César
C
Hernández-Rodríguez
Lourdes
L
Villa-Tanaca
Ignacio Eduardo
IE
Maldonado-Mendoza
Lidia
L
Dorantes-Álvarez
Darío
D
Tellez-Medina
María Eugenia
ME
Jaramillo-Flores
D000383:Agriculture; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D002212:Capsicum / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D002338:Carotenoids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D016000:Cluster Analysis; D018744:DNA, Plant / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D005819:Genetic Markers / Q000235:genetics; D008800:Mexico; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D025341:Principal Component Analysis; D019105:Random Amplified Polymorphic DNA Technique
D015415:Biomarkers; D018744:DNA, Plant; D005819:Genetic Markers; D010636:Phenols; D002338:Carotenoids