Determination of chrysene degradation under saline conditions by Fusarium sp. F092, a fungus screened from nature.
Sixty-two rotted wood and soil samples were used to screen for chrysene-degrading fungi. A strain of Fusarium, named F092, was identified as most capable of degrading chrysene. F092 was active under saline and nonsaline conditions, breaking down 48% of the chrysene in 30 d. The percentage of chrysene degraded did not change at 35‰ salinity with pH 8.2 in solid and liquid cultures. The degradation under saline conditions increased about 0.6- and 2.1-fold in cultures with polypeptone and Tween80, and 0.03-fold in agitated cultures. F092 secreted nonligninolytic enzymes named 1,2-dioxygenase and 2,3-dioxygenase. The level of 1,2-dioxygenase activity reached 203.5 U L(-1) at 30 d and that of 2,3-dioxygenase activity, 29.7 U L(-1) at 40 d. The degradation pathway was clarified from the intermediates produced; chrysene 1,2-oxide, chrysene trans-1,2-dihydrodiol, 1-hydroxy 2-naphtoic acid, and catechol. F092 is a potential degrader of chrysene for bioremediation.
United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi Matsuyama, Ehime 790-8566, Japan.
Asep
A
Hidayat
Sanro
S
Tachibana
Kazutaka
K
Itoh
D001711:Biotransformation; D002911:Chrysenes / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D049308:Dioxygenases / Q000378:metabolism; D005670:Fusarium / Q000302:isolation & purification* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D053858:Metabolic Networks and Pathways; D054712:Salinity; D012988:Soil Microbiology; D013997:Time Factors; D014934:Wood / Q000382:microbiology*
D002911:Chrysenes; D003470:Culture Media; C031180:chrysene; D049308:Dioxygenases