Successful nanolitre real-time PCR detection of respiratory pathogens in clinical specimens.
We performed a proof-of-concept study to determine if human pathogens could be detected in clinical specimens using nanolitre-volume real-time PCR. Nanolitre PCR for Bordetella pertussis/B. parapertussis and respiratory syncytial virus (RSV) was performed on nasopharyngeal specimens and results compared with conventional methods. B. pertussis/B. parapertussis nanolitre PCR detection was 100% sensitive (20/20; 95% CI, 84-100%) and 100% specific (26/26; 95% CI, 87-100%). RSV nanolitre PCR was also 100% sensitive (21/21; 95% CI, 85-100%) and specific (25/25; 95%, CI 87-100%). Respiratory pathogens can be successfully detected in clinical specimens using nanolitre-volume PCR.
Children's Hospital of Eastern Ontario, Ottawa, ON, Canada. slinger@cheo.on.ca
R
R
Slinger
I
I
Moldovan
N
N
Barrowman
F
F
Chan
D042483:Bordetella parapertussis / Q000235:genetics / Q000302:isolation & purification; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009305:Nasopharynx / Q000382:microbiology / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity