SHORT VEGETATIVE PHASE (SVP) protein negatively regulates miR172 transcription via direct binding to the pri-miR172a promoter in Arabidopsis.
Plant microRNAs (miRNAs) are non-coding RNAs that negatively regulate expression of their target genes. Although much is known about miRNA biogenesis and repression of target genes by miRNAs, the molecular mechanisms underlying the transcriptional regulation of miRNA itself are poorly understood. Here, we report that SHORT VEGETATIVE PHASE (SVP) protein is a direct transcriptional regulator of miR172. The levels of mature miR172 and pri-miR172a were anti-correlated with SVP activity. miR172a has multiple transcription start sites, among which the transcript starting with cytosine (-671bp, relative to the mature miR172a) was a major species. EMSA and ChIP analysis demonstrated that SVP protein binds to the CArG motifs in the miR172a promoter. These results suggest that SVP protein directly regulates miR172 transcription in Arabidopsis.
Creative Research Initiatives, Division of Life Sciences, Korea University, Seoul 136-701, Republic of Korea.
Hyun Jung
HJ
Cho
Jae Joon
JJ
Kim
Jeong Hwan
JH
Lee
Wanhui
W
Kim
Jae-Hoon
JH
Jung
Chung-Mo
CM
Park
Ji Hoon
JH
Ahn
D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D003596:Cytosine / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D035264:Flowers; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005982:Glutathione Transferase / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D035683:MicroRNAs; C543702:Mirn172 microRNA, Arabidopsis; C407549:SVP protein, Arabidopsis; D014157:Transcription Factors; D003596:Cytosine; D005982:Glutathione Transferase