Facilitated molecular typing of Shigella isolates using ERIC-PCR.
To evaluate the performance of enterobacterial repetitive intergenic sequence-based polymerase chain reaction (ERIC-PCR) typing versus the current standard for the typing of Shigella pulsed gel electrophoresis (PFGE), we typed 116 Shigella isolates from a village in an endemic setting over a 20-month period using both methods. PFGE identified 37 pulse types and had a discrimination index of 0.925 (95% confidence interval = 0.830-1.00), whereas ERIC-PCR identified 42 types and had a discrimination index of 0.961 (95% confidence interval = 0.886-1.00). PFGE and ERIC-PCR showed a 90.4% correlation in the designation of isolates as clonal or non-clonal in pairwise comparisons. Both systems were highly reproducible and provided highly similar and supplementary data compared with serotyping regarding the transmission dynamics of shigellosis in this community. ERIC-PCR is considerably more rapid and inexpensive than PFGE and may have a complementary role to PFGE for initial investigations of hypothesized outbreaks in resource-limited settings.
Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA. mkosek@jhsph.edu
Margaret
M
Kosek
Pablo Peñataro
PP
Yori
Robert H
RH
Gilman
Henry
H
Vela
Maribel Paredes
MP
Olortegui
Cesar Banda
CB
Chavez
Maritza
M
Calderon
Juan Perez
JP
Bao
Eric
E
Hall
Ryan
R
Maves
Rosa
R
Burga
Graciela Meza
GM
Sanchez
D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D021901:DNA, Intergenic / Q000378:metabolism; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D004405:Dysentery, Bacillary / Q000453:epidemiology / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D012091:Repetitive Sequences, Nucleic Acid; D012703:Serotyping; D012760:Shigella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D021901:DNA, Intergenic