WEFTA interlaboratory comparison on total lipid determination in fishery products using the Smedes method.
Lipid determination by the Smedes method was tested in an interlaboratory trial performed by nine laboratories from seven countries belonging to the West European Fish Technologists Association Analytical Methods Working Group. Five samples of fish and fishery products with different lipid contents, including two blind duplicates, were distributed among the participants. All laboratories applied a slightly modified Smedes method, which included extraction of lipids by cyclohexane and isopropanol, transfer of lipids to the cyclohexane phase by addition of water, phase separation by centrifugation, and gravimetric lipid determination. The results indicate that the RSD for reproducibility (RSD(R)) was between 4.11 and 6.31% for samples with moderate (7%) and high (14%) lipid content, depending on the sample. Larger SDs among the laboratories were obtained for a cod sample with low lipid content of 0.5%. The method is judged to be suitable as a routine method for lipid determination in fish and fishery products.
Max Rubner-Institute, Federal Research Institute of Nutrition and Food, Department of Safety and Quality of Milk and Fish Products, Palmaille 9, D-22767 Hamburg, Germany. Horst.Karl@mri.bund.de
Horst
H
Karl
Jörg
J
Oehlenschläger
Karen
K
Bekaert
Jean-Pascal
JP
Bergé
Asli
A
Cadun
Guillaume
G
Duflos
Bianca Maria
BM
Poli
Margarita
M
Tejada
Silvia
S
Testi
Maike
M
Timm-Heinrich
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods; D005399:Fishes; D005504:Food Analysis / Q000379:methods*; D007753:Laboratories / Q000592:standards*; D008055:Lipids / Q000737:chemistry*; D008460:Meat / Q000032:analysis*; D015588:Observer Variation
D008055:Lipids