Western blot analysis as an aid for the diagnosis of cutaneous leishmaniasis due to Leishmania major.
Cutaneous leishmaniasis (CL) due to Leishmania major is endemic in the Old World. To evaluate the diagnostic value of Western blot (WB) compared with IFAT, we tested serum samples from 45 patients with proven CL. Twenty-one (47%) patients were positive by IFAT and all patients were positive by WB with specific bands against 14kDa and/or 18kDa Leishmania antigens. Our results suggest that WB could be a useful non-invasive tool for the diagnosis of CL caused by L. major.
Faculté de Médecine, Université de Nice-Sophia Antipolis, Service de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Nice, Nice, France.
Christelle
C
Pomares
Laura
L
Despierres
Pascal
P
del Giudice
Pascal
P
Delaunay
Grégory
G
Michel
Bernard
B
Ferrua
Pierre
P
Marty
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D015153:Blotting, Western; D005260:Female; D006801:Humans; D018314:Leishmania infantum / Q000276:immunology / Q000302:isolation & purification*; D018320:Leishmania major / Q000276:immunology / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000473:pathology*; D008297:Male; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012867:Skin / Q000469:parasitology / Q000473:pathology*
D000913:Antibodies, Protozoan