Single step preparation of nanosilver loaded calcium phosphate by low temperature co-conversion process.
The preparation and characterization of nanosilver loaded calcium phosphate aiming to enhance the bactericidal performance by a single step co-conversion technique using low temperature ion exchange phosphorization in combination with Tollen's reaction were performed. Silver nitrate was used as a silver ion supply source (0.001-0.1 M) and glucose was employed as a reducing agent. After conversion, surface and shell zones of all samples comprised hydroxyapatite and metallic silver as the main phases regardless of silver nitrate concentration. However, hydroxyapatite, residual calcium sulfate and monetite were found in the core zone when using silver nitrate concentration lower than 0.1 M. The microstructure of all samples comprised the distribution of spherical-shaped silver nanoparticles within the cluster of calcium phosphate nanocrystals. Total silver content (range, 0.09-6.5 %) in the converted samples was found to linearly increase with increasing silver nitrate content. Flexural modulus and strength of converted samples generally decreased with increasing silver content. Effective antibacterial activity of two selected samples (0.001 and 0.005 M AgNO(3)) against two bacterial strains (Pseudomonas aeruginosa and Staphylococcus aureus) was observed. Cytotoxic potentials by MTT assay of both samples were observed at 24 and 48 h extraction respectively.
National Metal and Materials Technology Center, National Science and Technology Development Agency, Ministry of Science and Technology, 114 Paholyothin Road, Klong 1, Klongluang, Pathumthani, 12120, Thailand. jintamai@mtec.or.th
J
J
Suwanprateeb
F
F
Thammarakcharoen
K
K
Wasoontararat
W
W
Chokevivat
P
P
Phanphiriya
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000138:chemical synthesis* / Q000737:chemistry; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003080:Cold Temperature; D016503:Drug Delivery Systems; D006801:Humans; D008826:Microbial Sensitivity Tests; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology; D011208:Powders; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry*; D012835:Silver Nitrate / Q000008:administration & dosage / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D002130:Calcium Phosphates; D011208:Powders; D012834:Silver; D012835:Silver Nitrate