Multiplexed bead-based immunoassay of four serum biomarkers for diagnosis             of ovarian cancer.
The purpose of the present study was to evaluate multiplex liquid assay-based             measurement of multiple ovarian cancer-associated biomarkers such as hemoglobin,             haptoglobin and apolipoprotein E, together with CA125, which has been widely used             in the diagnosis of ovarian cancer, in order to provide a higher diagnostic power.             We measured the serum levels of CA125, hemoglobin, haptoglobin and apolipoprotein             E from the serum of 76 healthy individuals and 69 ovarian cancer patients using             a multiplex liquid assay system, Luminex 100. The results were analyzed according             to normal versus ovarian cancer, tumor stages and tumor histology. In addition,             to validate the use of these biomarkers for the diagnosis of ovarian cancer, the             sensitivity and specificity of each biomarker was analyzed by its receiver operating             characteristics (ROC) curve. The serum levels of all four biomarkers in ovarian             cancer patients were significantly higher than those of healthy individuals. When             CA125 was combined with the biomarkers, the overall sensitivity and specificity             were significantly improved in the ROC curve, which showed 95 and 75% sensitivity             and specificity, respectively. At 95% specificity for all stages the sensitivity             increased to 75% compared to 41% for CA125 alone. For stage I+II increased the             sensitivity to 68% from 36% for CA125 alone. For stage III+IV the corresponding             values were 100 and 95%, respectively. Taken together, the new combination of             hemoglobin, haptoglobin and apolipoprotein E with CA125 significantly improved             both the sensitivity and the specificity of ovarian cancer diagnosis compared             with those of individual biomarkers. These findings suggest the benefit of the             combination of these markers for the diagnosis of ovarian cancer.
Catholic Research Institutes of Medical Science, College of Medicine, The Catholic University of Korea, Gangnam Severance Hospital Biomedical Research Center, Seoul, Republic of Korea.
Yong-Wan
YW
Kim
Su Mi
SM
Bae
In-Wook
IW
Kim
Hai-Bo
HB
Liu
Hyo Joo
HJ
Bang
Pankaj Kumar
PK
Chaturvedi
Gantumur
G
Battogtokh
Hyunsun
H
Lim
Woong Shick
WS
Ahn
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D018394:CA-125 Antigen / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology; D016019:Survival Analysis; D055815:Young Adult
D014408:Biomarkers, Tumor; D018394:CA-125 Antigen