In vivo iontophoretic delivery of salmon calcitonin across microporated skin.
The purpose of this study was to determine the effect of microneedle (MN) technology and its combination with iontophoresis (ITP) on the in vivo transdermal delivery of salmon calcitonin (sCT). Maltose MNs (500 µm) were used to porate skin prior to application of the drug, with or without ITP. Micropores created by maltose MNs were characterized by histological sectioning and calcein imaging studies, which indicated uniformity of the created micropores. In vivo studies were performed in hairless rats to assess the degree of enhancement achieved by ITP (0.2 mA/cm² for 1 h), MNs (81 MNs), and their combination. In vivo studies indicate a serum maximal concentration of 0.61 ± 0.42 ng/mL, 1.79 ± 0.72 ng/mL, and 5.51 ± 0.32 ng/mL for ITP, MNs, and combination treatment, respectively. MN treatment alone increased serum concentration 2.5-fold and the combination treatment increased the concentration ninefold as compared with iontophoretic treatment alone. Combination treatment of ITP and MNs resulted in the highest delivery of sCT and therapeutic levels were achieved within 5 min of administration.
College of Pharmacy and Health Sciences, Mercer University, Atlanta, Georgia 30341, USA.
Viswatej
V
Vemulapalli
Yun
Y
Bai
Haripriya
H
Kalluri
Anushree
A
Herwadkar
Hyun
H
Kim
Shawn P
SP
Davis
Phil M
PM
Friden
Ajay K
AK
Banga
D000279:Administration, Cutaneous; D000818:Animals; D050071:Bone Density Conservation Agents / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D002116:Calcitonin / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems / Q000295:instrumentation*; D004867:Equipment Design; D007478:Iontophoresis / Q000295:instrumentation*; D008320:Maltose / Q000737:chemistry; D051381:Rats; D054772:Rats, Hairless; D012867:Skin / Q000378:metabolism*
D050071:Bone Density Conservation Agents; D008320:Maltose; C028815:salmon calcitonin; D002116:Calcitonin