High-throughput cellular screening of engineered ECM based on combinatorial polyelectrolyte multilayer films.
The capacity to engineer the extracellular matrix is critical to better understand cell function and to design optimal cellular environments to support tissue engineering, transplantation and repair. Stacks of adsorbed polymers can be engineered as soft wet three dimensional matrices, with properties tailored to support cell survival and growth. Here, we have developed a combinatorial method to generate coatings that self assemble from solutions of polyelectrolytes in water, layer by layer, to produce a polyelectrolyte multilayer (PEM) coating that has enabled high-throughput screening for cellular biocompatibility. Two dimensional combinatorial PEMs were used to rapidly identify assembly conditions that promote optimal cell survival and viability. Conditions were first piloted using a cell line, human embryonic kidney 293 cells (HEK 293), and subsequently tested using primary cultures of embryonic rat spinal commissural neurons. Cell viability was correlated with surface energy (wettability), modulus (matrix stiffness), and surface charge of the coatings.Our findings indicate that the modulus is a crucial determinant of the capacity of a surface to inhibit or support cell survival.
Department of Chemistry, Program in NeuroEngineering, McGill University, 801 Sherbrooke St. W., Montreal, Quebec H3A 2K6, Canada.
Miloslav
M
Sailer
Karen
K
Lai Wing Sun
Ozzy
O
Mermut
Timothy E
TE
Kennedy
Christopher J
CJ
Barrett
D000180:Acrylic Resins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism*; D004867:Equipment Design; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D057809:HEK293 Cells; D057166:High-Throughput Screening Assays / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008422:Materials Testing / Q000295:instrumentation* / Q000379:methods; D009474:Neurons / Q000166:cytology; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism; D051381:Rats
D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; D011073:Polyamines; C006903:carbopol 940