Detection of micrometastases in lung cancer with magnetic nanoparticles and quantum dots.
Detection of micrometastases plays an important role in early-stage and recurrent cancer diagnosis. In the study, a new method of screening micrometastases of lung cancer in peripheral blood by magnetic nanoparticles (MNPs) and quantum dots (QDs) was developed to achieve early diagnosis and recurrence prevention. MNPs were prepared by combining miniemulsion polymerization and Stöber coating methods. QDs were prepared by using Cd(Ac)(2) · 2H(2)O and oxygen-free NaHTe with thioglycolic acid as the stabilizer. The carbodiimide-mediated condensation method was used to couple pan-cytokeratin (pan-ck) antibody (Ab) to the surface of the MNPs, and Lunx and SP-A Abs to the surface of the QDs. After four kinds of epithelial tumor cells were enriched by MNPs coupled with pan-ck Ab (MNP-pan-ck), lung cancer cells A549 and SPC-A-1 were successfully identified by QDs with double-labeled Abs. Finally, 32 patients with non-small cell lung cancer (NSCLC) were collected, out of 26 cases with the enriched circulating tumor cells (CTCs), 21 cases were successfully identified by QDs. Therefore, a new method was established in which MNP-pan-ck collected CTCs and QDs with double-labeled Abs could be used simultaneously to identify CTCs from NSCLC patients.
Central Laboratory, China-Japan Union Hospital, Jilin University, Changchun, People's Republic of China.
Yali
Y
Wang
Yucheng
Y
Zhang
Zhenwu
Z
Du
Mei
M
Wu
Guizhen
G
Zhang
D000368:Aged; D019187:Cadmium Compounds / Q000737:chemistry; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000473:pathology*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008175:Lung Neoplasms / Q000097:blood* / Q000473:pathology*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D061206:Neoplasm Micrometastasis; D009360:Neoplastic Cells, Circulating / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D010750:Phosphoproteins / Q000378:metabolism; D037662:Pulmonary Surfactant-Associated Protein A / Q000378:metabolism; D045663:Quantum Dots; D013691:Tellurium / Q000737:chemistry
C485599:BPIFA1 protein, human; D019187:Cadmium Compounds; D006023:Glycoproteins; D058185:Magnetite Nanoparticles; D010750:Phosphoproteins; D037662:Pulmonary Surfactant-Associated Protein A; D007633:Keratins; D013691:Tellurium; C028337:cadmium telluride