Enhanced loading and controlled release of antibiotics using nucleic acids as an antibiotic-binding effector in hydrogels.
Antibiotic delivery is important to treat bacterial infections, one of the most challenging health problems globally. This study explored the application of nucleic acids as an antibiotic-binding effector for antibiotic loading and release. The data showed that the partition coefficient of tetracycline increased proportionally to the oligonucleotide concentration ranging from 0 to 1 mM. Resultantly, the incorporation of the oligonucleotides led to enhanced tetracycline loading in the hydrogels. In addition to the enhanced drug loading, the oligonucleotides could slow the release of tetracycline from the hydrogels. Experiments were further carried out to examine the capability of oligonucleotide-functionalized hydrogels in the inhibition of bacterial growth. The results showed that the oligonucleotide-functionalized hydrogels had higher antibacterial efficiency. Moreover, after tetracycline release, the oligonucleotide-functionalized hydrogels could be refilled with fresh tetracycline to reproduce the capability of inhibiting bacterial growth. Therefore, nucleic acid oligonucleotides are a promising antibiotic-binding effector for hydrogel functionalization in antibiotic delivery.
Program of Biomedical Engineering, School of Engineering, University of Connecticut, Storrs, Connecticut 06269, United States.
Xiaolong
X
Zhang
Boonchoy
B
Soontornworajit
Zhaoyang
Z
Zhang
Niancao
N
Chen
Yong
Y
Wang
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D018736:DNA Adducts / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D004339:Drug Compounding; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D020100:Hydrogels; D007700:Kinetics; D050296:Microbial Viability / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry*; D012212:Rheology; D013752:Tetracycline / Q000737:chemistry* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D018736:DNA Adducts; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D020100:Hydrogels; D011092:Polyethylene Glycols; D013752:Tetracycline