[Effects of dilution rate and carbon-to-nitrogen ratio on lipid accumulation by Rhodosporidium toruloides under chemostat conditions].
The objective of this work is to investigate how dilution rate and carbon-to-nitrogen (C/N) ratio affects lipid accumulation by Rhodosporidium toruloides AS 2.138 9 in continuous culture. Under steady-state conditions, the increase in dilution rate led to the decrease in lipid content and lipid yield. The highest lipid yield and lipid content at D = 0.02 h(-1) were 0.18 g lipid/g sugar and 57.1%, respectively, while the highest lipid productivity and biomass productivity were obtained at D = 0.14 h(-1). The increase in C/N ratio led to the increase in lipid content. The highest lipid content of 38% was obtained at C/N = 237. The highest lipid yield of 0.12 g lipid/g sugar was obtained at C/N = 92. However, the highest lipid productivity of 0.12 g/(L x h) was obtained at C/N = 32. No significant changes were observed in terms of fatty acid composition of the lipid produced under different C/N ratios, and these three fatty acids, palmitic acid, stearic acid and oleic acid, took over 85% in all samples.
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, Liaoning, China.
Hongwei
H
Shen
Guojie
G
Jin
Cuimin
C
Hu
Zhiwei
Z
Gong
Fengwu
F
Bai
Zongbao K
ZK
Zhao
D001487:Basidiomycota / Q000254:growth & development / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media; D005227:Fatty Acids / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism*; D019301:Oleic Acid / Q000096:biosynthesis; D019308:Palmitic Acid / Q000378:metabolism
D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D019301:Oleic Acid; D019308:Palmitic Acid; D002244:Carbon; D005947:Glucose; D009584:Nitrogen