Molecular and histological identification of the acanthocephalan Bolbosoma cf. capitatum from the human small intestine.
Acanthocephalans of the genus Bolbosoma are intestinal parasites of marine mammals with a lifecycle similar to that of anisakid nematodes. Several cases of Bolbosoma infection in humans have been reported, but no species identification has been made. Here, we report a case of Bolbosoma infection, in which the worm was found in histological sections of the partially resected small intestine of a Japanese man. Morphological features of the worm reconstructed from serial sectioning indicated that the worm was most likely to be a sexually immature female of Bolbosoma capitatum. DNA extraction from paraffin-embedded sections and ITS1-5.8S rRNA-ITS2 sequencing showed that this species formed a monophyletic group with Bolbosoma nipponicum, and was clearly distinguishable from Corynosoma spp. or Polymorphus spp. These results may provide a reference for identifying and characterizing unknown acanthocephalans found in histological sections.
Department of Infectious Diseases, Kyoto Prefectural University of Medicine, Kyoto, Japan. arizonon@koto.kpu-m.ac.jp
Naoki
N
Arizono
Toshiaki
T
Kuramochi
Noboru
N
Kagei
D000049:Acanthocephala / Q000145:classification* / Q000235:genetics; D000818:Animals; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005260:Female; D006373:Helminthiasis / Q000469:parasitology*; D006801:Humans; D007421:Intestine, Small / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D010802:Phylogeny
D021903:DNA, Ribosomal Spacer