[Study on extraction and immune regulation of polysaccharides from Marsdenia tenacissima].
OBJECTIVE
To optimize the extraction technology and determine the immune activity of polysaccharides from Marsdenia tenacissima.


METHODS
The optimum extraction technology of polysaccharide from M. tenacissima was detected by orthogonal experiments with the extraction rate of polysacchride and the total sugar content as indicators. The immunocompromised mice model was established by intraperitoneal injection cyclophosphamide to detected the content of IL-2, IL-6 in serum, CD4+, CD8+ in the peripheral blood by ELISA and flow cytometry, respectively.


RESULTS
By the extraction rate of polysaccharide, the sequence of seriousness of all affecting factors from high to low was extracting times, temperature, heating time and water ratio. By the total sugar content, the sequence was temperature, extracting times, water ratio and heating time. Compared with the model group, the pleen index, IL-2, IL-6, CD4/CD8+ were increased significantly (P < 0.05) in the 0.14 g x kg(-1) group and 0. 28 g x kg(-1) group.


CONCLUSIONS
T he optimum extraction condition was as follows: extraction three times/1.5 hours at 100 degrees C with 1:8 ratio of M. tenacissima to water. The polysaccharide of M. tenacissima can enhance the cellular immune and humoral immune.
Shanxi Institute of Traditional Chinese Medicine, Taiyuan 030012, China.
Xiufeng
X
Zhu
Yuanyuan
Y
Li
Yonghua
Y
Yue
Xianrong
X
Li
Xuliang
X
Hao
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D016516:CD4-CD8 Ratio; D005591:Chemical Fractionation / Q000379:methods*; D007376:Interleukin-2 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D031101:Marsdenia / Q000737:chemistry*; D051379:Mice; D011134:Polysaccharides / Q000276:immunology* / Q000302:isolation & purification* / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D013696:Temperature; D013997:Time Factors
D007376:Interleukin-2; D015850:Interleukin-6; D011134:Polysaccharides