Gene expression in visceral and subcutaneous adipose tissue in overweight women.
Excess weight and obesity are common health problems with multifactorial and polygenic causes. Abdominal or visceral obesity is associated with a higher risk of obesity related complications. The aim of the study was to evaluate differentially expressed genes in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) of 10 overweight women undergoing elective laparoscopic cholecystectomy. Following expression profiling using microarrays, a set of 294 genes that exhibited differential expression between VAT and SAT was further analyzed to test the functional correlation of gene sets using the Gene Set Enrichment Analysis method. To confirm the functional pathways involved in differential expression between SAT and VAT, additional pathway analysis was done using the GeneGo MetaCore software and the Ingenuity Pathway Analysis. Ten differentially expressed genes were selected according to the microarray data, with seven exhibiting significant differential expression in the RT-PCR experiments. The data from this preliminary study suggest enrichment of inflammation and oxidative stress related pathways in VAT, while insulin homeostasis pathways as well as pathways pertaining to several growth factors are enriched in SAT.
Department of Endocrinology and Metabolic Disorders, University Hospital Center Zagreb, Zagreb, Croatia.
Mirko
M
Korsic
Kristina
K
Gotovac
Matea
M
Nikolac
Tina
T
Dusek
Mate
M
Skegro
Dorotea
D
Muck-Seler
Fran
F
Borovecki
Nela
N
Pivac
D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D050177:Overweight / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D050151:Subcutaneous Fat / Q000378:metabolism*
D017931:DNA Primers