Knowledge-driven analysis identifies a gene-gene interaction affecting high-density lipoprotein cholesterol levels in multi-ethnic populations.
Total cholesterol, low-density lipoprotein cholesterol, triglyceride, and high-density lipoprotein cholesterol (HDL-C) levels are among the most important risk factors for coronary artery disease. We tested for gene-gene interactions affecting the level of these four lipids based on prior knowledge of established genome-wide association study (GWAS) hits, protein-protein interactions, and pathway information. Using genotype data from 9,713 European Americans from the Atherosclerosis Risk in Communities (ARIC) study, we identified an interaction between HMGCR and a locus near LIPC in their effect on HDL-C levels (Bonferroni corrected P(c) = 0.002). Using an adaptive locus-based validation procedure, we successfully validated this gene-gene interaction in the European American cohorts from the Framingham Heart Study (P(c) = 0.002) and the Multi-Ethnic Study of Atherosclerosis (MESA; P(c) = 0.006). The interaction between these two loci is also significant in the African American sample from ARIC (P(c) = 0.004) and in the Hispanic American sample from MESA (P(c) = 0.04). Both HMGCR and LIPC are involved in the metabolism of lipids, and genome-wide association studies have previously identified LIPC as associated with levels of HDL-C. However, the effect on HDL-C of the novel gene-gene interaction reported here is twice as pronounced as that predicted by the sum of the marginal effects of the two loci. In conclusion, based on a knowledge-driven analysis of epistasis, together with a new locus-based validation method, we successfully identified and validated an interaction affecting a complex trait in multi-ethnic populations.
Department of Biological Statistics and Computational Biology, Cornell University, Ithaca, New York, United States of America.
Li
L
Ma
Ariel
A
Brautbar
Eric
E
Boerwinkle
Charles F
CF
Sing
Andrew G
AG
Clark
Alon
A
Keinan
D001741:Black or African American; D008076:Cholesterol, HDL / Q000235:genetics*; D008078:Cholesterol, LDL / Q000235:genetics; D004843:Epistasis, Genetic; D055106:Genome-Wide Association Study; D006630:Hispanic or Latino; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics; D008049:Lipase / Q000235:genetics; D014280:Triglycerides / Q000235:genetics; D044465:White People
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; C504173:LIPC protein, human; D014280:Triglycerides; C553220:HMGCR protein, human; D006903:Hydroxymethylglutaryl CoA Reductases; D008049:Lipase