Kin selection, quorum sensing and virulence in pathogenic bacteria.
Bacterial growth and virulence often depends upon the cooperative release of extracellular factors excreted in response to quorum sensing (QS). We carried out an in vivo selection experiment in mice to examine how QS evolves in response to variation in relatedness (strain diversity), and the consequences for virulence. We started our experiment with two bacterial strains: a wild-type that both produces and responds to QS signal molecules, and a lasR (signal-blind) mutant that does not release extracellular factors in response to signal. We found that: (i) QS leads to greater growth within hosts; (ii) high relatedness favours the QS wild-type; and (iii) low relatedness favours the lasR mutant. Relatedness matters in our experiment because, at relatively low relatedness, the lasR mutant is able to exploit the extracellular factors produced by the cells that respond to QS, and hence increase in frequency. Furthermore, our results suggest that because a higher relatedness favours cooperative QS, and hence leads to higher growth, this will also lead to a higher virulence, giving a relationship between relatedness and virulence that is in the opposite direction to that usually predicted by virulence theory.
Department of Surgery, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
Kendra P
KP
Rumbaugh
Urvish
U
Trivedi
Chase
C
Watters
Maxwell N
MN
Burton-Chellew
Stephen P
SP
Diggle
Stuart A
SA
West
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D005260:Female; D008099:Liver / Q000382:microbiology; D051379:Mice; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D053038:Quorum Sensing; D012641:Selection, Genetic; D012867:Skin / Q000382:microbiology; D015534:Trans-Activators / Q000235:genetics; D014774:Virulence; D014947:Wounds and Injuries / Q000382:microbiology / Q000473:pathology
D001426:Bacterial Proteins; C080365:LasR protein, Pseudomonas aeruginosa; D015534:Trans-Activators