Reactivity of nucleosides with a hydroxyl radical in non-aqueous medium.
DNA damage: The reactivity of HO(.) with silylated 2'-deoxyribonucleosides was investigated in acetonitrile by means of a time-resolved technique. The obtained rate constants were in general slightly lower than those reported for the natural nucleosides in water. Analysis of the reaction mixture by UPLC-MS revealed that HO(.) attack occurred at the nucleobase (see scheme).
Instituto de Tecnología Química UPV-CSIC, Universidad Politécnica de Valencia, Consejo Superior de Investigaciones Científicas, Avenida de los Naranjos, s/n, 46022 Valencia, Spain.
Gemma M
GM
Rodríguez-Muñiz
M Luisa
ML
Marin
Virginie
V
Lhiaubet-Vallet
Miguel A
MA
Miranda
D000097:Acetonitriles / Q000737:chemistry*; D004249:DNA Damage; D003853:Deoxyribonucleosides / Q000138:chemical synthesis / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry*; D015394:Molecular Structure; D009705:Nucleosides / Q000737:chemistry*; D055668:Photochemical Processes; D012821:Silanes / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D003853:Deoxyribonucleosides; D009705:Nucleosides; D012821:Silanes; D012997:Solvents; D014867:Water; D017665:Hydroxyl Radical