Selenium distribution and speciation in the hyperaccumulator Astragalus bisulcatus and associated ecological partners.
The goal of this study was to investigate how plant selenium (Se) hyperaccumulation may affect ecological interactions and whether associated partners may affect Se hyperaccumulation. The Se hyperaccumulator Astragalus bisulcatus was collected in its natural seleniferous habitat, and x-ray fluorescence mapping and x-ray absorption near-edge structure spectroscopy were used to characterize Se distribution and speciation in all organs as well as in encountered microbial symbionts and herbivores. Se was present at high levels (704-4,661 mg kg(-1) dry weight) in all organs, mainly as organic C-Se-C compounds (i.e. Se bonded to two carbon atoms, e.g. methylselenocysteine). In nodule, root, and stem, up to 34% of Se was found as elemental Se, which was potentially due to microbial activity. In addition to a nitrogen-fixing symbiont, the plants harbored an endophytic fungus that produced elemental Se. Furthermore, two Se-resistant herbivorous moths were discovered on A. bisulcatus, one of which was parasitized by a wasp. Adult moths, larvae, and wasps all accumulated predominantly C-Se-C compounds. In conclusion, hyperaccumulators live in association with a variety of Se-resistant ecological partners. Among these partners, microbial endosymbionts may affect Se speciation in hyperaccumulators. Hyperaccumulators have been shown earlier to negatively affect Se-sensitive ecological partners while apparently offering a niche for Se-resistant partners. Through their positive and negative effects on different ecological partners, hyperaccumulators may influence species composition and Se cycling in seleniferous ecosystems.
Department of Biology, Colorado State University, Fort Collins, Colorado 80523, USA.
José R
JR
Valdez Barillas
Colin F
CF
Quinn
John L
JL
Freeman
Stormy D
SD
Lindblom
Sirine C
SC
Fakra
Matthew A
MA
Marcus
Todd M
TM
Gilligan
Élan R
ÉR
Alford
Ami L
AL
Wangeline
Elizabeth A H
EA
Pilon-Smits
D000818:Animals; D027883:Astragalus Plant / Q000378:metabolism*; D017753:Ecosystem; D035264:Flowers / Q000033:anatomy & histology / Q000378:metabolism; D060434:Herbivory / Q000502:physiology; D007814:Larva / Q000502:physiology; D008954:Models, Biological; D009036:Moths / Q000502:physiology; D009928:Organ Specificity; D012639:Seeds / Q000033:anatomy & histology / Q000378:metabolism; D012643:Selenium / Q000378:metabolism*; D013052:Spectrometry, X-Ray Emission; D013455:Sulfur / Q000378:metabolism; D056928:X-Ray Absorption Spectroscopy
D013455:Sulfur; D012643:Selenium