Assessment of ruminal hydrogen sulfide or urine thiosulfate as diagnostic tools for sulfur induced polioencephalomalacia in cattle.
To determine if ruminal hydrogen sulfide, urine thiosulfate, or blood sulfhemoglobin could be used as diagnostic indicators for sulfur-induced polioencephalomalacia, 16 steers (8 cannulated, 368 ± 12 kg; 8 unmodified, 388 ± 10 kg; mean ± standard error) were fed 1 of 2 dietary treatments. Diets consisted of a low sulfate (0.24% S; control) wheat midd-based pellet or the control pellet with sodium sulfate added to achieve a high-sulfate (0.68% S) pellet. As designed, intake did not differ (P = 0.80) between treatments. At 8 hr postfeeding, ruminal hydrogen sulfide was not affected by cannulation (P = 0.35) but was greater (P < 0.01) in high S (6,005 ± 475 mg/l) than control (1,639 ± 472 mg/l) steers. Time of day of sampling affected (P = 0.01) ruminal hydrogen sulfide, with peak concentrations occurring 4-12 hr after feeding. Urine was collected prefeeding (AM) and 7-9 hr postfeeding (PM). Urine thiosulfate concentrations of high S steers sampled in the PM were greater (P > 0.01) than in the AM. However, there was no difference due to time of sampling for control. In both the AM and PM, urine thiosulfate concentrations of high S were greater (P > 0.01) than control. Although hydrogen sulfide and thiosulfate were elevated by increased dietary S intake, a concentration at which polioencephalomalacia is likely to occur could not be determined. Sampling urine for thiosulfate or rumen gas for hydrogen sulfide of nonsymptomatic pen mates 4-8 hr after feeding may be useful to assess sulfur exposure and differentiate between causes of polioencephalomalacia.
Department of Animal Science, Iowa State University, 313F Kildee Hall, Ames, IA 50011-3150, USA.
Mary E
ME
Drewnoski
Steve M
SM
Ensley
Don C
DC
Beitz
Jon P
JP
Schoonmaker
Dan D
DD
Loy
Paula M
PM
Imerman
John A
JA
Rathje
Stephanie L
SL
Hansen
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism* / Q000652:urine; D004678:Encephalomalacia / Q000175:diagnosis / Q000378:metabolism / Q000652:urine / Q000662:veterinary*; D006862:Hydrogen Sulfide / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008297:Male; D011897:Random Allocation; D012417:Rumen / Q000378:metabolism*; D013431:Sulfates / Q000008:administration & dosage / Q000378:metabolism* / Q000633:toxicity*; D013435:Sulfhemoglobin / Q000032:analysis*; D013885:Thiosulfates / Q000652:urine*
D013431:Sulfates; D013885:Thiosulfates; D013435:Sulfhemoglobin; D006862:Hydrogen Sulfide