Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase.
The Ca(2+)-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoH(V83F), was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoH(V83F) mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH.
Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
Taísa
T
Magnani Dinamarco
Neil Andrew
NA
Brown
Ricardo Sergio
RS
Couto de Almeida
Patrícia
P
Alves de Castro
Marcela
M
Savoldi
Maria Helena
MH
de Souza Goldman
Gustavo Henrique
GH
Goldman
D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics; D019703:Calcineurin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D020134:Catalytic Domain; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009701:Nucleoside-Diphosphate Kinase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
D005656:Fungal Proteins; D009701:Nucleoside-Diphosphate Kinase; D019703:Calcineurin; D002118:Calcium