[Determination of phenobarbital in human urine and serum using flow injection chemiluminescence].
A sensitive chemiluminescence method, based on the enhancive effect of phenobarbital on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of phenobarbital. The chemiluminescence intensity responded to the concentration of phenobarbital linearly ranging from 0.05 to 10 ng x ml(-1) with the detection limit of 0.02 ng x ml(-1) (3 sigma). At a flow rate of 2.0 ml x min(-1), a complete determination of phenobarbital, including sampling and washing, could be accomplished in 0.5 min, offering the sampling efficiency of 120 h(-1) accordingly. The method was applied successfully in an assay of PB for pharmaceutical preparations, human urine and serum without any pretreatment with recovery from 95.7 to 106.7% and RSDs of less than 3.0%.
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Niu
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He
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Song
D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D010634:Phenobarbital / Q000032:analysis* / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results
D008165:Luminol; D010634:Phenobarbital