Responsive behavior of tumor-marker-imprinted hydrogels using macromolecular cross-linkers.
Tumor-marker-imprinted hydrogels having lectin and antibody molecules as ligands for a tumor-specific marker glycoprotein were strategically prepared by biomolecular imprinting using minute amounts of low-molecular-weight or high-molecular-weight cross-linkers. The tumor-marker-imprinted hydrogels shrank gradually in response to a target glycoprotein, because their apparent cross-linking density increased owing to simultaneous complex formation of lectin and antibody ligands with a target glycoprotein after their ligands dynamically recognized the glycoprotein. The swelling ratio of the tumor-marker-imprinted hydrogel using high-molecular-weight cross-linker with an optimal chain length decreased more drastically than that using a low-molecular-weight cross-linker, but the hydrogel using the cross-linker with a chain that was too long did not exhibit tumor-marker responsive behavior. This paper focuses on the effect of the molecular weight of cross-linkers on the responsive behavior of tumor-marker-imprinted hydrogels having lectin and antibody molecules as ligands. The cross-linker chain length was an important factor in determining the dynamic glycoprotein recognition and responsive behavior of the biomolecule-imprinted hydrogels.
Department of Chemistry and Materials Engineering, Kansai University, Suita, Osaka, 564-8680, Japan. tmiyata@kansai-u.ac.jp
Takashi
T
Miyata
Takeshi
T
Hayashi
Yoshiaki
Y
Kuriu
Tadashi
T
Uragami
D000178:Acrylamides / Q000737:chemistry; D000327:Adsorption; D000912:Antibodies, Neoplasm / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry; D019245:Compressive Strength; D003208:Concanavalin A / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D008958:Models, Molecular; D054802:Molecular Imprinting / Q000379:methods; D008970:Molecular Weight; D009369:Neoplasms / Q000175:diagnosis; D011092:Polyethylene Glycols / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000737:chemistry
D000178:Acrylamides; D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D003432:Cross-Linking Reagents; D020100:Hydrogels; D008689:Methacrylates; D000509:alpha-Fetoproteins; C421283:poly(ethylene glycol)-dimethacrylate; C437167:poly(ethylene glycol)diacrylate; D003208:Concanavalin A; D011092:Polyethylene Glycols; C021221:N,N'-methylenebisacrylamide