Discrepancy between minimal inhibitory concentration to enrofloxacin and mutations present in the quinolone-resistance determining regions of Mycoplasma gallisepticum field strains.
Molecular characterization of the quinolone-resistance determining regions (QRDRs) of DNA gyrase and topoisomerase IV in 93 Mycoplasma gallisepticum field strains isolated in different geographic regions revealed discrepancies between minimal inhibitory concentration values and presence of amino acid substitutions within the QRDRs of GyrA and ParC in 9/93 (10%) strains. This may delimitate applicability of a gene-based assay to detect fluoroquinolone resistance in this avian pathogen.
Mycoplasma Unit, Kimron Veterinary Institute, Bet Dagan, Israel. innal@moag.gov.il
I
I
Lysnyansky
I
I
Gerchman
S
S
Levisohn
I
I
Mikula
A
A
Feberwee
N M
NM
Ferguson
A H
AH
Noormohammadi
J
J
Spergser
H M
HM
Windsor
D019943:Amino Acid Substitution; D000818:Animals; D001717:Birds; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000737:chemistry / Q000235:genetics; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009175:Mycoplasma Infections / Q000382:microbiology; D045686:Mycoplasma gallisepticum / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D011201:Poultry Diseases / Q000382:microbiology; D015363:Quinolones / Q000494:pharmacology
D024841:Fluoroquinolones; D015363:Quinolones; D000077422:Enrofloxacin; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase