Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium.
A novel Gram-stain-positive actinobacterium, designated RI148-Li105(T), was isolated from a lichen sample from Rishiri Island, Japan, and its taxonomic position was investigated by a polyphasic approach. 16S rRNA gene sequencing study indicated that strain RI148-Li105(T) was related to the type strain of Luteimicrobium subarcticum, with a similarity of 97.8%. Cells of strain RI148-Li105(T) exhibited a rod-coccus cycle. The diagnostic cell-wall diamino acid of this organism was lysine and the peptidoglycan type was found to be A4α. The predominant menaquinones were MK-8(H(2)) and MK-9(H(2)), and the major fatty acids were iso-C(16:0), C(17:1) ω9c and C(17:0). The DNA G+C content was 73.6 mol%. The major phenotypic characteristics of strain RI148-Li105(T) basically corresponded to those of the genus Luteimicrobium excluding the fatty acid composition. These results suggest that strain RI148-Li105(T) should be affiliated with the genus Luteimicrobium. Meanwhile, DNA-DNA hybridization and some phenotypic characteristics revealed that the strain differs from L. subarcticum. Therefore, strain RI148-Li105(T) represents a novel species of the genus Luteimicrobium, for which the name Luteimicrobium album sp. nov. is proposed. The type strain of Luteimicrobium album is RI148-Li105(T) (=NBRC 106348(T)=DSM 24866(T)).
NITE Biological Resource Center, National Institute of Technology and Evaluation, Kisarazu, Chiba, Japan. hamada-moriyuki@nite.go.jp
Moriyuki
M
Hamada
Hideki
H
Yamamura
Chiaki
C
Komukai
Tomohiko
T
Tamura
Ken-ichiro
K
Suzuki
Masayuki
M
Hayakawa
D039903:Actinobacteria / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D000596:Amino Acids / Q000032:analysis; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007564:Japan; D008011:Lichens / Q000382:microbiology*; D008855:Microscopy, Electron, Scanning; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000032:analysis
D000596:Amino Acids; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2