[Enzyme-linked immunosorbent assay for determination of cyclosporin A in whole blood].
A test-system based on enzyme-linked immunosorbent assay (ELISA) for quantitative determination of cyclosporin A (CSA) in human whole blood has been developed. The detection limit of the method was 25 ng/ml, the linearity of the method in the concentration range 60-1400 ng/ml varied from 94 to 105%, the variation coefficient did not exceed 8%. The novel method exhibited good correlation with radio-immunoassay and polarization fluoroimmunoassay methods; the linear regression coefficients were 0.965 and 0.983, respectively. The developed test system is stable for at least 9 months when stored at 4 degrees C and can be used in clinical practice.
I P
IP
Andreeva
N T
NT
Vorob'eva
L I
LI
Vinnitskiĭ
S S
SS
Bogush
E M
EM
Gavrilova
A M
AM
Egorov
D000911:Antibodies, Monoclonal; D016572:Cyclosporine / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood*; D057230:Limit of Detection; D011933:Reagent Kits, Diagnostic
D000911:Antibodies, Monoclonal; D007166:Immunosuppressive Agents; D011933:Reagent Kits, Diagnostic; D016572:Cyclosporine