YqjD is an inner membrane protein associated with stationary-phase ribosomes in Escherichia coli.
Here, we provide evidence that YqjD, a hypothetical protein of Escherichia coli, is an inner membrane and ribosome binding protein. This protein is expressed during the stationary growth phase, and expression is regulated by stress response sigma factor RpoS. YqjD possesses a transmembrane motif in the C-terminal region and associates with 70S and 100S ribosomes at the N-terminal region. Interestingly, E. coli possesses two paralogous proteins of YqjD, ElaB and YgaM, which are expressed and bind to ribosomes in a similar manner to YqjD. Overexpression of YqjD leads to inhibition of cell growth. It has been suggested that YqjD loses ribosomal activity and localizes ribosomes to the membrane during the stationary phase.
Department of Physics, Osaka Medical College, Takatsuki, Osaka, Japan. yhide@art.osaka-med.ac.jp
Hideji
H
Yoshida
Yasushi
Y
Maki
Shou
S
Furuike
Akiko
A
Sakai
Masami
M
Ueta
Akira
A
Wada
D001426:Bacterial Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism*; D012808:Sigma Factor / Q000378:metabolism
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D012808:Sigma Factor; C043426:inner membrane protein, E coli; C069567:sigma factor KatF protein, Bacteria