[Detection of recombinant-DNA in foods from stacked genetically modified plants].
A quantitative real-time multiplex polymerase chain reaction method was applied to the detection and quantification of MON863 and MON810 in stacked genetically modified maize MON 810xMON 863. The limit of detection was approximately 0,1%. The accuracy of the quantification, measured as bias from the accepted value and the relative repeatability standard deviation, which measures the intra-laboratory variability, were within 25% at each GM-level. A method verification has demonstrated that the MON 863 and the MON810 methods can be equally applied in quantification of the respective events in stacked MON810xMON 863.
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Sorokina
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Chernyshova
D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis*; D005433:Flour / Q000032:analysis; D030841:Food, Genetically Modified / Q000592:standards*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics*; D012680:Sensitivity and Specificity; D003313:Zea mays / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant