Influenza A viruses control expression of proviral human p53 isoforms p53β and Delta133p53α.
Previous studies have described the role of p53 isoforms, including p53β and Δ133p53α, in the modulation of the activity of full-length p53, which regulates cell fate. In the context of influenza virus infection, an interplay between influenza viruses and p53 has been described, with p53 being involved in the antiviral response. However, the role of physiological p53 isoforms has never been explored in this context. Here, we demonstrate that p53 isoforms play a role in influenza A virus infection by using silencing and transient expression strategies in human lung epithelial cells. In addition, with the help of a panel of different influenza viruses from different subtypes, we also show that infection differentially regulates the expressions of p53β and Δ133p53α. Altogether, our results highlight the role of p53 isoforms in the viral cycle of influenza A viruses, with p53β and Δ133p53α acting as regulators of viral production in a p53-dependent manner.
Division of Medical Science, Centre for Oncology and Molecular Medicine, University of Dundee, Ninewells Hospital, Dundee, Scotland, United Kingdom. olivier.terrier@univ-lyon1.fr
Olivier
O
Terrier
Virginie
V
Marcel
Gaëlle
G
Cartet
David P
DP
Lane
Bruno
B
Lina
Manuel
M
Rosa-Calatrava
Jean-Christophe
JC
Bourdon
D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D006801:Humans; D009980:Influenza A virus / Q000472:pathogenicity*; D007251:Influenza, Human; D020033:Protein Isoforms / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
D020033:Protein Isoforms; D016159:Tumor Suppressor Protein p53