Comparison of Ki-67, cyclin E, and p63 in benign and malignant human pleomorphic adenoma.
OBJECTIVE
A set of cell proliferation-related molecular markers was used to detect the differences in proliferative activity between benign pleomorphic adenoma (PA) and carcinoma ex pleomorphic adenoma (CXPA).


METHODS
Seventy samples comprising 53 PAs, 10 noninvasive and 7 widely invasive (WI) CXPAs were selected. Immunohistochemical staining was used to detect Ki-67, cyclin E, and p63 expression.


RESULTS
The average Ki-67 labeling index of WI CXPAs was higher than that of PAs and the malignant component of noninvasive CXPAs (M-noninvasive CXPA; P < .01). An increased cyclin E-positive staning was observed in M-noninvasive CXPAs and WI CXPAs compared with PAs (P = .006). The p63 expression levels were notably decreased in M-noninvasive CXPAs and WI CXPAs compared with PAs (P = .007).


CONCLUSIONS
The data support that increased or decreased expression of Ki-67, cyclin E, and p63 may indicate the proliferative differences between PA and CXPA.
State Key Laboratory Breeding Base of Basic Science of Stomatology, School of Stomatology, Wuhan University, China.
Xiaojie
X
Zhu
Jiali
J
Zhang
Xinming
X
Chen
Xiaojie
X
Feng
D008949:Adenoma, Pleomorphic / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002471:Cell Transformation, Neoplastic; D016009:Chi-Square Distribution; D019927:Cyclin E / Q000096:biosynthesis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D018709:Statistics, Nonparametric; D014157:Transcription Factors / Q000096:biosynthesis; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis; D055815:Young Adult
D019927:Cyclin E; D019394:Ki-67 Antigen; C115051:TP63 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins