The influence of the organic modifier in hydro-organic mobile phase on separation selectivity of steroid hormones separation using cholesterol-bonded stationary phases.
Chromatographic properties of four cholesterol bonded phases with different structures were studied. The columns used were packed with a stationary phase containing a cholesterol molecule attached to the silica surface using different types of linkage molecules. Columns were compared according to the retention and separation selectivity of steroid hormones. The measurements were done using binary hydro-organic mobile phases with methanol, ethanol and acetonitrile as an organic modifier. The presented results show that the coverage density of the bonded ligands and the type of organic modifier strongly influence the retention mechanism and separation selectivity of steroid hormones on cholesterol-containing adsorbents.
Chair of Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St., PL-87-100 Toruń, Poland.
Szymon
S
Bocian
Jan
J
Soukup
Maria
M
Matyska
Joseph
J
Pesek
Pavel
P
Jandera
Bogusław
B
Buszewski
D000327:Adsorption; D002784:Cholesterol / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry; D013256:Steroids / Q000032:analysis*
D013256:Steroids; D012822:Silicon Dioxide; D002784:Cholesterol