Expression of 14-3-3γ in patients with breast cancer: correlation with clinicopathological features and prognosis.
OBJECTIVE
Protein 14-3-3γ is an important member of the 14-3-3 family that play important roles in the regulation of various cellular processes. The aim of the study is to investigate the association between 14-3-3γ expression and the clinicopathological features of patients with breast cancer.


METHODS
The expression of 14-3-3γ was detected by Western blot in both foci of breast cancer and adjacent non-cancerous tissues. In addition, 14-3-3γ expression was analyzed by immunohistochemistry in 60 clinicopathologically characterized breast cancer cases. The association of 14-3-3γ expression with survival of the patients were analyzed.


RESULTS
The expression level of 14-3-3γ protein in breast cancer were significantly higher than that in non-cancerous mammary gland tissues. Moreover, high expression of 14-3-3γ correlated with tumor size and tumor grade (all P<0.05). Patients with high 14-3-3γ expression had worse overall survival rate than that with low expression (P < 0.05). Furthermore, multivariate analysis showed that 14-3-3γ expression was an independent predictor of overall survival (HR, 0.196; 95%CI, 0.043-0.892; P = 0.035).


CONCLUSIONS
Our data suggest for the first time that the increased expression of 14-3-3γ in breast cancer is associated significantly with tumor progression and poor prognosis. 14-3-3γ may be a novel and potential prognostic marker for breast cancer.
Department of Pathology, The first affiliated hospital of Guang Dong Pharmaceutical University, Guangzhou, China. songyulan1@126.com
Yulan
Y
Song
Zheng
Z
Yang
Zunfu
Z
Ke
Yunhong
Y
Yao
Xinrong
X
Hu
Yanqin
Y
Sun
Hongmei
H
Li
Jinbao
J
Yin
Chao
C
Zeng
D048948:14-3-3 Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011379:Prognosis; D015996:Survival Rate
D048948:14-3-3 Proteins; D014408:Biomarkers, Tumor