Use of matrix assisted laser desorption ionisation-time of flight mass spectrometry in a paediatric clinical laboratory for identification of bacteria commonly isolated from cystic fibrosis patients.
BACKGROUND
Matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) has been described as a rapid, accurate method for bacterial identification.


OBJECTIVE
To investigate the ability of the technique, using the unamended database supplied with the system, to identify bacteria commonly isolated in cystic fibrosis (CF) patients.


METHODS
Organisms commonly isolated from CF patients identified by MALDI-TOF MS were compared to conventional phenotypic and genotypic analyses. For MALDI-TOF MS, the direct colony technique was used routinely with one extraction procedure performed on a mucoid Pseudomonas aeruginosa. For 24 unique CF specimens, workload comparison and time to identification were assessed.


RESULTS
Of 464 tested isolates, conventional (phenotypic and genotypic) identification compared to MALDI-TOF MS showed complete genus, species agreement in 92%, with genus agreement in 98%. This included 29 isolates within the Burkholderia cepacia complex. All 29 were correctly identified to the genus level and 24 of these were speciated. Time to identification with 47 bacterial isolates from 24 CF patients showed identification of 85% of isolates by MALDI-TOF MS at 48 h of incubation, compared to only 34% with conventional methods.


CONCLUSIONS
Using the unamended database supplied with the system, MALDI-TOF MS provides rapid and reliable identification of bacteria isolated from CF specimens. Time to identification studies showed that the use of same day, same method for organism identification will decrease time to result and optimise microbiology workflow.
Department of Pediatric Infectious Diseases, Emory University School of Medicine, 2015 Uppergate Drive, Atlanta, GA 30322, USA. ankita.desai@emory.edu
Ankita Patel
AP
Desai
Theresa
T
Stanley
Maria
M
Atuan
Jonelle
J
McKey
John J
JJ
Lipuma
Beverly
B
Rogers
Robert
R
Jerris
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis / Q000382:microbiology*; D001431:Bacteriological Techniques; D019121:Burkholderia Infections / Q000175:diagnosis / Q000382:microbiology; D042602:Burkholderia cepacia complex / Q000235:genetics / Q000302:isolation & purification; D019411:Clinical Laboratory Techniques; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology*; D005838:Genotype; D005845:Georgia; D006801:Humans; D010641:Phenotype; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D013996:Time and Motion Studies; D057188:Workflow