An evaluation of the Shandon Papspin liquid-based oral test using a novel cytologic scoring system.
OBJECTIVE
The aim of this study was to evaluate a cost-effective oral liquid-based cytology screening test, Shandon Papspin (PS).


METHODS
We compared the diagnostic accuracy of PS with surgical biopsy in 69 patients. Transepithelial cytology specimens were obtained with the use of cervical cytobrushes. Cytology specimens were graded and scored by using a novel oral cytologic grading and scoring system.


RESULTS
Histologic diagnosis of dysplasia or malignancy was made in 51/69 cases, and cytology identified 49/51 cases, with a sensitivity of 96% and specificity of 100%. The best cutoff value for distinguishing reactive/mildly dysplastic lesions from high grade/invasive squamous cell carcinoma was determined to be a cytologic score of 3, representing a sensitivity of 95% and specificity of 96%.


CONCLUSIONS
PS appears to be an accurate and economical test for detection of high-risk dysplasias and cancers, but the real significance of this method will be its diagnostic accuracy in studies focusing strictly on lesions with a low level of clinical concern.
Department of Oral Pathology, University of the Western Cape and National Health Laboratory Services, Tygerberg Hospital, Cape Town, South Africa. amir.afrogheh@nhls.ac.za
Amir
A
Afrogheh
Colleen Anne
CA
Wright
Sean Liam
SL
Sellars
Julie
J
Wetter
Andrew
A
Pelser
Pawel Tomasz
PT
Schubert
Jos
J
Hille
D001706:Biopsy / Q000379:methods; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002498:Centrifugation; D003362:Cost-Benefit Analysis; D003581:Cytodiagnosis / Q000295:instrumentation / Q000379:methods*; D004847:Epithelial Cells / Q000473:pathology*; D006801:Humans; D007202:Indicators and Reagents; D008403:Mass Screening / Q000379:methods; D009061:Mouth Mucosa / Q000473:pathology*; D009062:Mouth Neoplasms / Q000473:pathology*; D060787:Neoplasm Grading / Q000379:methods; D009959:Oropharyngeal Neoplasms / Q000473:pathology; D012680:Sensitivity and Specificity
D007202:Indicators and Reagents