The lymphocyte crossmatch by flow cytometry for kidney transplantation.
The flow cytometric lymphocyte crossmatch is a standard technique for evaluating the compatibility of potential kidney transplant recipients and donors. Recipient serum is incubated with donor lymphocytes and the latter are analysed in a flow cytometer for the presence of bound IgG antibodies. An increase in the level of IgG binding compared to a negative control indicates the presence of donor-specific antibodies which may lead to deleterious graft function. Described here is a method to perform T and B lymphocyte crossmatching in the same tube to detect IgG donor-reactive antibodies.
Tissue Typing Laboratory, New Zealand Blood Service, Auckland, New Zealand. jonathan.downing@nzblood.co.nz
Jonathan
J
Downing
D000906:Antibodies / Q000032:analysis / Q000276:immunology; D001788:Blood Grouping and Crossmatching; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*
D000906:Antibodies; D007074:Immunoglobulin G