The effect of deacetylated gellan gum on aesculin distribution in the posterior segment of the eye after topical administration.
The aim of the present work was to evaluate the effect of deacetylated gellan gum on delivering hydrophilic drug to the posterior segment of the eye. An aesculin-containing in situ gel based on deacetylated gellan gum (AG) was prepared and characterized. In vitro corneal permeation across isolated rabbit cornea of aesculin between AG and aesculin solution (AS) was compared. The results showed that deacetylated gellan gum promotes corneal penetration of aesculin. Pharmacokinetics and ocular tissue distribution of aesculin after topical administration in rabbit eye showed that AG greatly improved aesculin accumulation in posterior segmentsrelative to AS, which was probably attributed to conjunctivital/sclera pathway. The area-under-the-curve (AUC) for AG in aqueous humor, choroid-retina, sclera and iris-ciliary body were significantly larger than those of AS. AG can be used as a potential carrier for broading the application of aesculin.
College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu, China. cqhhong_617@126.com
Qiuhong
Q
Chen
Yu
Y
Zheng
Ye
Y
Li
Ying
Y
Zeng
Jianchao
J
Kuang
Shixiang
S
Hou
Xiaohui
X
Li
D000107:Acetylation; D000287:Administration, Topical; D000818:Animals; D003315:Cornea / Q000187:drug effects* / Q000378:metabolism*; D004338:Drug Combinations; D004347:Drug Interactions / Q000502:physiology; D004929:Esculin / Q000008:administration & dosage* / Q000378:metabolism*; D005123:Eye / Q000187:drug effects / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage* / Q000378:metabolism*; D011817:Rabbits; D011897:Random Allocation; D014018:Tissue Distribution / Q000187:drug effects / Q000502:physiology; D016896:Treatment Outcome
D004338:Drug Combinations; D011135:Polysaccharides, Bacterial; D004929:Esculin; C048288:gellan gum