Toxicological effect of herbicides (diuron and bentazon) on snake venom and electric eel acetylcholinesterase.
The toxicological effects of the active ingredients of the herbicides diuron and bentazon on the activity of acetylcholinesterase (AChE) of krait (Bungarus sindanus) venom and electric eel (Electrophorus electricus) were studied. The diuron and entazon caused non-competitive inhibition of AChE from both species. For the venom AChE, the calculated IC50 for diuron and bentazon were found to be 3.25 and 0.14 μM, while for eel AChE, the respective IC50 values were 3.6 and 0.135 μM. In comparison, bentazon was a more potent inhibitor than diuron of AChE from both species. The insecticide lindane did not have any inhibitory effect on AChE activity in either species, even when tested at high concentrations (200-800 μM).
Department of Biotechnology, University of Science and Technology, Bannu, KPK, Pakistan. mushtaq213@yahoo.com
Mushtaq
M
Ahmed
Nadia
N
Latif
Rehmat Ali
RA
Khan
Akhlaq
A
Ahmad
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001581:Benzothiadiazines / Q000633:toxicity*; D017838:Bungarus / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000633:toxicity; D004237:Diuron / Q000633:toxicity*; D004593:Electrophorus / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D006540:Herbicides / Q000633:toxicity*; D001556:Hexachlorocyclohexane / Q000633:toxicity; D020128:Inhibitory Concentration 50; D007306:Insecticides / Q000633:toxicity; D012910:Snake Venoms / Q000378:metabolism*
D001581:Benzothiadiazines; D002800:Cholinesterase Inhibitors; D006540:Herbicides; D007306:Insecticides; D012910:Snake Venoms; D001556:Hexachlorocyclohexane; D004237:Diuron; D000110:Acetylcholinesterase; C002003:bentazone