Multimodal photoacoustic ophthalmoscopy in mouse.
Photoacoustic ophthalmoscopy (PAOM) is a novel imaging technology that measures optical absorption in the retina. The capability of PAOM can be further enhanced if it could image mouse eyes, because mouse models are widely used for various retinal diseases. The challenges in achieving high-quality imaging of mouse retina, however, come from the much smaller eyeball size. Here, we report an optimized imaging system, which integrates PAOM, spectral-domain optical coherence tomography (SD-OCT), and autofluorescence-scanning laser ophthalmoscopy (AF-SLO), for mouse eyes. Its multimodal capability was demonstrated by imaging transgenic Nrl-GFP mice that express green fluorescent protein (GFP) in photoreceptors. SD-OCT provided guidance of optical alignment for PAOM and AF-SLO, and complementary contrast with high depth-resolution retinal cross sections. PAOM visualized the retinal vasculature and retinal pigment epithelium melanin, and AF-SLO measured GFP-expressing in retinal photoreceptors. The in vivo imaging results were verified by histology and confocal microscopy.
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd., Evanston IL 60208, USA.|Department of Physics, Harbin Institute of Technology, 92 West Da-Zhi Street Nangang District, Harbin, Heilongjiang, P.R. China 150080.
Wei
W
Song
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd., Evanston IL 60208, USA.
Qing
Q
Wei
Department of Ophthalmology, Northwestern University, 645 North Michigan Ave., Chicago IL 60611, USA.
Liang
L
Feng
Department of Ophthalmology, Northwestern University, 645 North Michigan Ave., Chicago IL 60611, USA.
Vijay
V
Sarthy
Department of Ophthalmology, University of Southern California, 1450 San Pablo Street, Los Angeles CA 90033, USA.
Shuliang
S
Jiao
Department of Ophthalmology, Northwestern University, 645 North Michigan Ave., Chicago IL 60611, USA.
Xiaorong
X
Liu
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd., Evanston IL 60208, USA.|Department of Ophthalmology, Northwestern University, 645 North Michigan Ave., Chicago IL 60611, USA.
Hao F
HF
Zhang
D000818:Animals; D005654:Fundus Oculi; D051379:Mice; D008822:Mice, Transgenic; D064847:Multimodal Imaging / Q000379:methods*; D009887:Ophthalmoscopy / Q000379:methods*; D061088:Photoacoustic Techniques / Q000379:methods*; D041623:Tomography, Optical Coherence