Laboratory assessment of uptake and toxicity of lanthanum (La) in the leaves of Hydrocharis dubia (Bl.) Backer.
Increasing amounts of lanthanum (La) is released into aquatic environments. However, little information is available on the influence of La on aquatic plants. In this study, physiological and ultrastructural responses of Hydrocharis dubia (Bl.) Backer leaves to elevated concentrations of La (up to 160 μM) were investigated. The accumulation of La was found to be increased in a concentration-dependent manner. La disturbed the intrinsic balance of nutrient elements (P, Mg, Ca, Fe, K, and Zn). Pigment content decreased with the rise of the La concentrations and the EC(50) value for chlorophyll was 20 μM on day 7. The antioxidants (superoxide dismutase, peroxidase, catalase, reduced ascorbate, and reduced glutathione) exhibited varied response to the La treatments. Malondialdehyde content enhanced gradually at all La concentrations. The enhancement in proline content was found in a concentration-dependent manner. The amounts of three polypeptides with apparent molecular weights of 61.9, 51.5, and 16.7 kDa, respectively, were gradually diminished, as well as one existing polypeptides with apparent molecular weight of 22.3 kDa, elevating in response to increasing La concentrations. Significant damage to the chloroplast, mitochondrion, and nucleus was imposed by La indicated a general disarray in the cellular functions. The negative effects of La on H. dubia unequivocally indicate that La could exert an adverse influence on aquatic ecosystem and should lead to a more careful discharge of such elements into water environment.
Institute of Plant Cell and Molecular Biology, College of Life Science, Nanjing Normal University, Nanjing, 210046, People's Republic of China. xuqinsong@163.com
Qinsong
Q
Xu
Yongyang
Y
Fu
Haili
H
Min
Sanjuan
S
Cai
Sha
S
Sha
Guangyu
G
Cheng
D001205:Ascorbic Acid; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D031321:Hydrocharitaceae / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D007811:Lanthanum / Q000378:metabolism* / Q000633:toxicity; D008315:Malondialdehyde; D009195:Peroxidase / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity
D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D008315:Malondialdehyde; D007811:Lanthanum; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid