Simplified rat lung transplantation using a new cuff technique.
We introduce a new procedure for orthotopic left lung transplantation in rats. First, cuffs are attached to vessels or bronchi in both donors and recipients. Next, the cuffs are anastomosed by interposing pieces of the donor's descending aorta. Anastomosis time is markedly reduced to approximately 10 minutes. Transplantations were completed in 8 rats without technical errors. This rat lung transplantation technique is a straightforward method any surgeon can perform.
Department of General Thoracic Surgery, School of Medicine, Keio University, Tokyo, Japan. taichiro@1997.jukuin.keio.ac.jp
Taichiro
T
Goto
Mitsutomo
M
Kohno
Masaki
M
Anraku
Takashi
T
Ohtsuka
Yotaro
Y
Izumi
Hiroaki
H
Nomori
D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D001011:Aorta / Q000601:surgery*; D001807:Blood Vessel Prosthesis; D001980:Bronchi / Q000601:surgery*; D057785:Catheters; D008168:Lung / Q000098:blood supply*; D016040:Lung Transplantation / Q000379:methods*; D008297:Male; D008866:Microsurgery / Q000379:methods; D011651:Pulmonary Artery / Q000601:surgery*; D011667:Pulmonary Veins / Q000601:surgery*; D051381:Rats; D011917:Rats, Inbred Lew