Development of a fluorescent chalcone library and its application in the discovery of a mouse embryonic stem cell probe.
We report the first fluorescent diamino-chalcone library and its application in the discovery of a mouse embryonic stem cell (mESC) probe. CDg4, a novel green fluorescent mESC probe was discovered through a high-content image based screening of 160 members of the chalcone library. Interestingly, the molecular binding target of CDg4 was identified as the glycogen of the stem cell colony surface, rather than a conventional protein target from an intracellular source.
Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Biopolis, Singapore 138667.
Sung-Chan
SC
Lee
Nam-Young
NY
Kang
Sung-Jin
SJ
Park
Seong-Wook
SW
Yun
Yogeswari
Y
Chandran
Young-Tae
YT
Chang
D000818:Animals; D002599:Chalcone / Q000737:chemistry* / Q000493:pharmacokinetics; D053595:Embryonic Stem Cells / Q000737:chemistry / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000493:pharmacokinetics; D051379:Mice; D018613:Microscopy, Confocal; D015394:Molecular Structure; D054852:Small Molecule Libraries / Q000737:chemistry
D005456:Fluorescent Dyes; D054852:Small Molecule Libraries; D002599:Chalcone