Biomarker identification for prostate cancer and lymph node metastasis from microarray data and protein interaction network using gene prioritization method.
Finding a genetic disease-related gene is not a trivial task. Therefore, computational methods are needed to present clues to the biomedical community to explore genes that are more likely to be related to a specific disease as biomarker. We present biomarker identification problem using gene prioritization method called gene prioritization from microarray data based on shortest paths, extended with structural and biological properties and edge flux using voting scheme (GP-MIDAS-VXEF). The method is based on finding relevant interactions on protein interaction networks, then scoring the genes using shortest paths and topological analysis, integrating the results using a voting scheme and a biological boosting. We applied two experiments, one is prostate primary and normal samples and the other is prostate primary tumor with and without lymph nodes metastasis. We used 137 truly prostate cancer genes as benchmark. In the first experiment, GP-MIDAS-VXEF outperforms all the other state-of-the-art methods in the benchmark by retrieving the truest related genes from the candidate set in the top 50 scores found. We applied the same technique to infer the significant biomarkers in prostate cancer with lymph nodes metastasis which is not established well.
Institute of Information Systems and Applications, National Tsing Hua University, Hsinchu 30013, Taiwan. carlos.r.arias@gmail.com
Carlos Roberto
CR
Arias
Hsiang-Yuan
HY
Yeh
Von-Wun
VW
Soo
D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics / Q000503:physiopathology*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D060066:Protein Interaction Maps / Q000235:genetics / Q000502:physiology*; D049490:Systems Biology / Q000379:methods
D014408:Biomarkers, Tumor