Presence of CSE1L protein in urine of patients with urinary bladder urothelial carcinomas.
The chromosome segregation 1-like (CSE1L) protein is highly expressed in most cancers and has been shown to be secreted by tumor cells. We studied the presence of CSE1L in the urine of patients with bladder urothelial carcinomas. The results of our immunohistochemical analysis showed a high expression of CSE1L in bladder cancer specimens, while the normal bladder specimens only showed a very faint staining in some cells. Immunoblotting showed that CSE1L was present in urine of patients with bladder cancer. Urinary CSE1L-positive cases were detected in 95% (57/60) of patients with bladder urothelial carcinomas or the atypical/suspicious cases with urothelial atypia. No CSE1L was detected in urine of healthy controls (p<0.01). Our results suggest that urinary CSE1L deserves further evaluation for the screening of bladder cancer.
Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan.
Cheng-Jeng
CJ
Tai
Ching-Fong
CF
Liao
Tzu-Cheng
TC
Su
Ko-Hung
KH
Shen
Chun-Chao
CC
Chang
Shu-Hui
SH
Lin
Chung-Min
CM
Yeh
Shing-Chuan
SC
Shen
Woan-Ruoh
WR
Lee
Jeng-Fong
JF
Chiou
Chih-Hung
CH
Lin
Ying-Chun
YC
Chen
Hsin-Yi
HY
Shih
Kun-Tu
KT
Yeh
Ming-Chung
MC
Jiang
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000652:urine*; D002295:Carcinoma, Transitional Cell / Q000473:pathology / Q000652:urine*; D029563:Cellular Apoptosis Susceptibility Protein / Q000652:urine*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D001749:Urinary Bladder Neoplasms / Q000473:pathology / Q000652:urine*
D014408:Biomarkers, Tumor; D029563:Cellular Apoptosis Susceptibility Protein