Chitosan encapsulated quantum dots platform for leukemia detection.
We report results of the studies relating to electrophoretic deposition of nanostructured composite of chitosan (CS)-cadmium-telluride quantum dots (CdTe-QDs) onto indium-tin-oxide coated glass substrate. The high resolution transmission electron microscopic studies of the nanocomposite reveal molecular level coating of the CdTe-QDs with CS molecules in the colloidal dispersion medium. This novel composite platform has been explored to fabricate an electrochemical DNA biosensor for detection of chronic myelogenous leukemia (CML) by immobilizing amine terminated oligonucleotide probe sequence containing 22 base pairs, identified from BCR-ABL fusion gene. The results of differential pulse voltammetry reveal that this nucleic acid sensor can detect as low as 2.56 pM concentration of complementary target DNA with a response time of 60s. Further, the response characteristics show that this fabricated bioelectrode has a shelf life of about 6 weeks and can be used for about 5-6 times. The results of experiments conducted using clinical patient samples reveal that this sensor can be used to distinguish CML positive and the negative control samples.
Department of Science & Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, Materials Physics & Engineering Division, National Physical Laboratory (Council of Scientific & Industrial Research), Dr K.S. Krishnan Marg, New Delhi 110012, India.
Aditya
A
Sharma
Chandra Mouli
CM
Pandey
Gajjala
G
Sumana
Udit
U
Soni
Sameer
S
Sapra
A K
AK
Srivastava
Tathagat
T
Chatterjee
Bansi D
BD
Malhotra
D001483:Base Sequence; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D055664:Electrochemical Techniques / Q000191:economics / Q000379:methods; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000235:genetics; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry; D045663:Quantum Dots; D013691:Tellurium / Q000737:chemistry; D013997:Time Factors; D017971:Tin Compounds / Q000737:chemistry
D019187:Cadmium Compounds; D015342:DNA Probes; D017971:Tin Compounds; C109984:indium tin oxide; D048271:Chitosan; D016044:Fusion Proteins, bcr-abl; D013691:Tellurium; C028337:cadmium telluride