Expression of CCR7 and CD45RA in CD4+ and CD8+ subsets in cerebrospinal fluid of 134 patients with inflammatory and non-inflammatory neurological diseases.
We investigated CD45RA and CCR7 expression in CD4+ and CD8+ subsets of cerebrospinal fluid (CSF) lymphocytes, both immediately ex vivo and after stimulation, from 134 patients with a variety of inflammatory and non-inflammatory neurological diseases. Most inflammatory diseases had a higher CD4+:CD8+ ratio and higher percentage of effector memory T cells (T(EM)) than non-inflammatory controls, excluding active infection. Moreover, we found that patients with highly elevated cell counts in the CSF tended to have a lower percentage of central memory T cells (T(CM)) than patients with low or absent pleocytosis, with a concomitant increase in T(EM). We also found that samples with elevated IgG index or presence of oligoclonal bands had a significantly higher CD4+:CD8+ ratio than normal samples, consistent with increased CD4+ help for intrathecal IgG synthesis by B cells.
Johns Hopkins School of Medicine, Department of Neurology, 600 N. Wolfe St., Baltimore, MD 21287, United States.
Katherine M
KM
Mullen
Anne R
AR
Gocke
Rameeza
R
Allie
Achilles
A
Ntranos
Inna V
IV
Grishkan
Carlos
C
Pardo
Peter A
PA
Calabresi
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000276:immunology / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D007223:Infant; D007249:Inflammation / Q000134:cerebrospinal fluid / Q000276:immunology / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000096:biosynthesis / Q000134:cerebrospinal fluid / Q000276:immunology; D008297:Male; D008875:Middle Aged; D054400:Receptors, CCR7 / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
C515370:CCR7 protein, human; D007074:Immunoglobulin G; D054400:Receptors, CCR7; D017493:Leukocyte Common Antigens