Traditional medicinal herbs and food plants have the potential to inhibit key carbohydrate hydrolyzing enzymes in vitro and reduce postprandial blood glucose peaks in vivo.
We hypothesized that some medicinal herbs and food plants commonly used in the management of diabetes can reduce glucose peaks by inhibiting key carbohydrate hydrolyzing enzymes. To this effect, extracts of Antidesma madagascariense (AM), Erythroxylum macrocarpum (EM), Pittosporum senacia (PS), and Faujasiopsis flexuosa (FF), Momordica charantia (MC), and Ocimum tenuiflorum (OT) were evaluated for α-amylase and α-glucosidase inhibitory effects based on starch-iodine colour changes and PNP-G as substrate, respectively. Only FF and AM extracts/fractions were found to inhibit α-amylase activity significantly (P < 0.05) and coparable to the drug acarbose. Amylase bioassay on isolated mouse plasma confirmed the inhibitory potential of AM and FF extracts with the ethyl acetate fraction of FF being more potent (P < 0.05) than acarbose. Extracts/fractions of AM and MC were found to inhibit significantly (P < 0.05) α-glucosidase activity, with IC(50) comparable to the drug 1-deoxynojirimycin. In vivo studies on glycogen-loaded mice showed significant (P < 0.05) depressive effect on elevation of postprandial blood glucose following ingestion of AM and MC extracts. Our findings tend to provide a possible explanation for the hypoglycemic action of MC fruits and AM leaf extracts as alternative nutritional therapy in the management of diabetes.
Department of Health Sciences, Faculty of Science, University of Mauritius, Reduit 230, Mauritius. f.mahomoodally@uom.ac.mu
M Fawzi
MF
Mahomoodally
A Hussein
AH
Subratty
A
A
Gurib-Fakim
M Iqbal
MI
Choudhary
S
S
Nahar Khan
D000818:Animals; D019659:Asteraceae / Q000737:chemistry; D001786:Blood Glucose / Q000187:drug effects*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D051379:Mice; D031339:Ocimum / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D010945:Plants, Edible / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D019518:Postprandial Period / Q000187:drug effects; D019685:Rosales / Q000737:chemistry; D000516:alpha-Amylases / Q000378:metabolism*; D000520:alpha-Glucosidases / Q000378:metabolism*
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D000516:alpha-Amylases; D000520:alpha-Glucosidases