A simple and sensitive method for determining the strand orientation of single-stranded viral genomes.
Determining the nature of the viral genome is one of the first steps in characterization of any new virus. However, in the case of viruses with a single-stranded genome, it is not always simple to identify its orientation. In this study, a rapid, sensitive and simple PCR-based method is described to identify the strand orientation of single-stranded viral genomes. This method has been tested on the single-stranded DNA viruses, M13 and phiX174.
Institut Pasteur, Department of Microbiology, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, 75015 Paris, France. tomohiro.mochizuki@pasteur.fr
Tomohiro
T
Mochizuki
David
D
Prangishvili
D017104:Bacteriophage M13 / Q000235:genetics*; D010584:Bacteriophage phi X 174 / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics*; D005827:Genetics, Microbial / Q000379:methods*; D016679:Genome, Viral; D008967:Molecular Biology / Q000379:methods*; D014773:Virology / Q000379:methods*
D004277:DNA, Single-Stranded