Biologically active steroids from the aerial parts of Vernonia anthelmintica Willd.
A new steroid, vernoanthelsterone A (1), and five known steroids were isolated from the aerial parts of Vernonia anthelmintica Willd. Compound 1 possesses a Δ(8(14))-15-one moiety. To our best knowledge, few steroids with this moiety have been reported before. Compounds 1-6 were tested for their antibacterial activities and their effects on estrogen biosynthesis in human ovarian granulosa-like cells (KGN cells). Compound 2 showed the ability to promote estrogen biosynthesis with EC(50) of 56.95 μg/mL and also exhibited the antibacterial activities against Bacillus cereus, Staphyloccocus aureus, Bacillus subtilis and Escherichia coli with MICs ranging from 3.15 to 15.5 μg/mL. The structures of 1-6 were determined on the basis of IR, MS, 1D and 2D NMR.
State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, PR China.
Lei
L
Hua
Ya
Y
Li
Fei
F
Wang
Dan-feng
DF
Lu
Kun
K
Gao
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001407:Bacillus / Q000187:drug effects; D002460:Cell Line; D004926:Escherichia coli / Q000187:drug effects; D004967:Estrogens / Q000096:biosynthesis*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D035741:Vernonia / Q000737:chemistry*
C576424:24xi-hydroperoxy-24-vinyllathosterol; D000900:Anti-Bacterial Agents; D004967:Estrogens; D010936:Plant Extracts; D013256:Steroids; C576423:vernoanthelsterone A