Passive immunization with a monoclonal IgM antibody specific for Strongyloides ratti HSP60 protects mice against challenge infection.
It is estimated that 30-100 million people are infected with the pathogenic nematode Strongyloides stercoralis worldwide but parasite control is still based on anti-helminthic treatment. To develop protective vaccination strategies, we use the murine model of Strongyloides ratti infection. We have shown recently that vaccination with alum-precipitated, but not with native or CFA-emulsified S. ratti heat shock protein 60 (srHSP60) conferred protection to challenge infection. Here we describe the generation of a monoclonal IgM specific for srHSP60. Anti-srHSP60 detected human and srHSP60 and stained S. ratti infective larvae in vitro. Passive immunization of mice with monoclonal anti-srHSP60 IgM led to reduced numbers of migrating larvae in lung and head, reduced numbers of parasitic adults in the small intestine and reduced larval output upon S. ratti challenge infection. Taken together, our findings highlight the relevance of srHSP60 as vaccine candidate for the induction of antibody-mediated protection against Strongyloides infection.
Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht Str. 74, 20359 Hamburg, Germany.
Nadia
N
Ben Nouir
Melanie
M
Piédavent
Anke
A
Osterloh
Minka
M
Breloer
D000818:Animals; D000909:Antibodies, Helminth / Q000008:administration & dosage* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D018834:Chaperonin 60 / Q000276:immunology; D004195:Disease Models, Animal; D005243:Feces / Q000469:parasitology; D005260:Female; D007116:Immunization, Passive / Q000379:methods*; D007075:Immunoglobulin M / Q000008:administration & dosage* / Q000276:immunology; D007421:Intestine, Small / Q000469:parasitology; D051379:Mice; D008810:Mice, Inbred C57BL; D059208:Parasite Load; D017170:Strongyloides ratti / Q000276:immunology; D013322:Strongyloidiasis / Q000276:immunology / Q000517:prevention & control*; D016896:Treatment Outcome
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D018834:Chaperonin 60; D007075:Immunoglobulin M