High-resolution protein structure determination by serial femtosecond crystallography.
Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.
Linac Coherent Light Source, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA. sboutet@slac.stanford.edu
Sébastien
S
Boutet
Lukas
L
Lomb
Garth J
GJ
Williams
Thomas R M
TR
Barends
Andrew
A
Aquila
R Bruce
RB
Doak
Uwe
U
Weierstall
Daniel P
DP
DePonte
Jan
J
Steinbrener
Robert L
RL
Shoeman
Marc
M
Messerschmidt
Anton
A
Barty
Thomas A
TA
White
Stephan
S
Kassemeyer
Richard A
RA
Kirian
M Marvin
MM
Seibert
Paul A
PA
Montanez
Chris
C
Kenney
Ryan
R
Herbst
Philip
P
Hart
Jack
J
Pines
Gunther
G
Haller
Sol M
SM
Gruner
Hugh T
HT
Philipp
Mark W
MW
Tate
Marianne
M
Hromalik
Lucas J
LJ
Koerner
Niels
N
van Bakel
John
J
Morse
Wilfred
W
Ghonsalves
David
D
Arnlund
Michael J
MJ
Bogan
Carl
C
Caleman
Raimund
R
Fromme
Christina Y
CY
Hampton
Mark S
MS
Hunter
Linda C
LC
Johansson
Gergely
G
Katona
Christopher
C
Kupitz
Mengning
M
Liang
Andrew V
AV
Martin
Karol
K
Nass
Lars
L
Redecke
Francesco
F
Stellato
Nicusor
N
Timneanu
Dingjie
D
Wang
Nadia A
NA
Zatsepin
Donald
D
Schafer
James
J
Defever
Richard
R
Neutze
Petra
P
Fromme
John C H
JC
Spence
Henry N
HN
Chapman
Ilme
I
Schlichting
D000818:Animals; D018360:Crystallography, X-Ray / Q000379:methods*; D007834:Lasers; D009113:Muramidase / Q000737:chemistry / Q000528:radiation effects; D011487:Protein Conformation
C421864:hen egg lysozyme; D009113:Muramidase