Oxidative stress in growth hormone transgenic coho salmon with compressed lifespan--a model for addressing aging.
Growth hormone (GH) transgenic fish have dramatically enhanced growth rates, increased oxygen demands and reactive oxygen species production. GH-transgenic coho salmon provide an opportunity to address effects of increased metabolism on physiological aging. The objective of this study was to compare oxidative stress in wild-type (WT) and GH-transgenic (T) coho salmon (Oncorhynchus kisutch) of different ages (1 and 2 years). Antioxidant enzyme activity, protein carbonyls (PC) and glutathione (GSH, GSSG) were measured. PC correlated to growth rates in individual fish. T fish exhibited lower antioxidant enzyme activities and GSH levels compared to the WT, while levels of PC and GSSG were higher. Age affects were observed in both WT and T fish; enzyme activities and GSH decreased while PC and GSSG increased. Our results support the metabolic rate theory of aging. This study aims to be a platform for continued studies of the theories of aging using fish as model organisms.
University of Gothenburg, Department of Biological and Environmental Sciences, Göteborg, Sweden. bethanie.carney@zool.gu.se
Bethanie
B
Carney Almroth
Jörgen I
JI
Johnsson
Robert
R
Devlin
Joachim
J
Sturve
D000375:Aging / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D000975:Antioxidants / Q000378:metabolism; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D023421:Models, Animal; D017684:Oncorhynchus kisutch / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D017382:Reactive Oxygen Species; D013006:Growth Hormone