Synthesis and characterization of PEG-based drug-responsive biohybrid hydrogels.
Interactive materials being responsive to a biocompatible stimulus represent a promising approach for future therapeutic applications. In this study, we present a novel biohybrid material synthesized from biocompatible components being stimulus-responsive to the pharmaceutically approved small-molecule novobiocin. The hydrogel design is based on the gyrase B (GyrB) protein, which is covalently grafted to multi-arm polyethylene glycol (PEG) using a Michael-type addition reaction. Upon addition of the GyrB-dimerizing substance coumermycin, stable hydrogels form which can be dissolved in a dose-adjustable manner by the antibiotic novobiocin. The switchable properties of this PEG-based hydrogel are favorable for future applications in tissue engineering and as externally controlled drug depot.
Faculty of Biology, Centre for Biological Signalling Studies (BIOSS) and Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.
Raphael J
RJ
Gübeli
Martin
M
Ehrbar
Martin
M
Fussenegger
Christian
C
Friedrich
Wilfried
W
Weber
D049933:Aminocoumarins / Q000737:chemistry*; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D027081:DNA Gyrase / Q000737:chemistry / Q000378:metabolism; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D009675:Novobiocin / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D059005:Topoisomerase II Inhibitors
D049933:Aminocoumarins; D001672:Biocompatible Materials; D020100:Hydrogels; D059005:Topoisomerase II Inhibitors; D009675:Novobiocin; D011092:Polyethylene Glycols; D027081:DNA Gyrase; C004628:coumermycin