[The cultivation of bone marrow cells and cell lines on polymeric films].
The cultivation of multipotent mesenchymal stromal bone marrow cells and cells of A-431, MDCK, Vero, 3T3 and Hep-G2 was performed on polymeric films (PVA) with different hydrophobic fatty acid residues. The cells of different types grew on these films with different intensity, but in the most cases comparable with the cultivation control on usual plastic. The examined films were nontoxic to cells and sufficiently adhesive. They did not changed pH of cultural media, were optically transparent under microscope and comfortable in the experimental work. These films can be used as a model for the artificial organ construction. The covalent binding of different fatty acids to PVA shows possibility of the adaptable changes of films properties (hydrophobity and adhesiveness), and therefore possibility of the creation of optimal conditions for different cell types attachement and growth.
M S
MS
Dolgikh
D N
DN
Livak
M E
ME
Krasheninnikov
N A
NA
Onishchenko
D016475:3T3 Cells; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002417:Cattle; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002470:Cell Survival; D002522:Chlorocebus aethiops; D003470:Culture Media / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D000882:Haplorhini; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D051381:Rats; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D014709:Vero Cells
D003470:Culture Media; D005227:Fatty Acids; D011142:Polyvinyl Alcohol