Collagen-based fibrillar multilayer films cross-linked by a natural agent.
Surface functionalization plays an important role in the design of biomedical implants, especially when layer forming cells, such as endothelial or epithelial cells, are needed. In this study, we define a novel nanoscale surface coating composed of collagen/alginate polyelectrolyte multilayers and cross-linked for stability with genipin. This buildup follows an exponential growth regime versus the number of deposition cycles with a distinct nanofibrillar structure that is not damaged by the cross-linking step. Stability and cell compatibility of the cross-linked coatings were studied with human umbilical vein endothelial cells. The surface coating can be covered by a monolayer of vascular endothelial cells within 5 days. Genipin cross-linking renders the surface more suitable for cell attachment and proliferation compared to glutaraldehyde (more conventional cross-linker) cross-linked surfaces, where cell clumps in dispersed areas were observed. In summary, it is possible with the defined system to build fibrillar structures with a nanoscale control of film thickness, which would be useful for in vivo applications such as inner lining of lumens for vascular and tracheal implants.
Institut National de la Santé et de la Recherche Médicale , INSERM UMR 977, Biomaterials and Tissue Engineering, Strasbourg, France.
Christophe
C
Chaubaroux
Engin
E
Vrana
Christian
C
Debry
Pierre
P
Schaaf
Bernard
B
Senger
Jean-Claude
JC
Voegel
Youssef
Y
Haikel
Christian
C
Ringwald
Joseph
J
Hemmerlé
Philippe
P
Lavalle
Fouzia
F
Boulmedais
D000464:Alginates / Q000737:chemistry; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry*; D024022:Fibrillar Collagens / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D039823:Iridoids / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D057139:Nanofibers / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry
D000464:Alginates; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D024022:Fibrillar Collagens; D006603:Hexuronic Acids; D039823:Iridoids; D020723:Glucuronic Acid; C007834:genipin; D005976:Glutaral