[Differentiation of human amniotic mesenchymal stem cells into insulin-secreting cells induced by regenerating pancreatic extract].
In this study, the natural biological inducer, rat regenerating pancreatic extract (RPE), was used to induce human amniotic mesenchymal stem cells (hAMSCs) into insulin-secreting cells. We excised 60% of rat pancreas in order to stimulate pancreatic regeneration. RPE was extracted and used to induce hAMSCs at a final concentration of 20 microg/mL. The experiment methods used were as follows: morphological-identification, dithizone staining, immumofluorescence analysis, reverse transcription-PCR (RT-PCR) and insulin secretion stimulated by high glucose. The results show that the cell morphology of passge3 hAMSCs changed significantly after the induction of RPE, resulting in cluster shape after induction for 15 days. Dithizone staining showed that there were scarlet cell masses in RPE-treated culture. Immumofluorescence analysis indicated that induced cells were insulin-positive expression. RT-PCR showed the positive expression of human islet-related genes Pdx1 and insulin in the induced cells. The result of insulin secretion stimulated by high glucose indicated that insulin increasingly secreted and then kept stable with prolongation of high glucose stimulation. In conclusion, hAMSCs had the potential to differentiate into insulin-secreting cells induced by RPE in vitro.
Central Laboratory, Second Artillery General Hospital, Beijing 100088, China.
Yanmei
Y
Zhang
Dianliang
D
Wang
Hongyan
H
Zeng
Lieming
L
Wang
Jinwei
J
Sun
Zhen
Z
Zhang
Shasha
S
Dong
D000650:Amnion / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D050417:Insulin-Secreting Cells / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010179:Pancreas / Q000502:physiology / Q000601:surgery; D010184:Pancreatic Extracts / Q000494:pharmacology*; D051381:Rats; D012038:Regeneration
D010184:Pancreatic Extracts