Immunohistochemical staining of dpERK staining during early kidney development.
Signaling through the ERK/MAPK pathway within the Wolffian duct and ureteric bud epithelium is critical for kidney induction and branching morphogenesis. ERK signaling is activated by receptor tyrosine kinase such as RET and FGFR2. This protocol describes a method to detect the diphosphorylated form of ERK (dpERK) on paraffin embedded tissue of early mouse embryo.
Department of Genetics and Development, Columbia University, New York, NY, USA.
Xuan
X
Chi
Odyssé
O
Michos
D000818:Animals; D016616:Cryoultramicrotomy / Q000379:methods; D004622:Embryo, Mammalian / Q000196:embryology / Q000201:enzymology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000032:analysis*; D007120:Immunochemistry / Q000379:methods*; D007668:Kidney / Q000196:embryology* / Q000201:enzymology*; D051379:Mice; D016612:Paraffin Embedding / Q000379:methods; D010766:Phosphorylation
D048049:Extracellular Signal-Regulated MAP Kinases