Encapsulation of basic fibroblast growth factor by polyelectrolyte multilayer microcapsules and its controlled release for enhancing cell proliferation.
Basic fibroblast growth factor (FGF2) is an important protein for cellular activity and highly vulnerable to environmental conditions. FGF2 protected by heparin and bovine serum albumin was loaded into the microcapsules by a coprecipitation-based layer-by-layer encapsulation method. Low cytotoxic and biodegradable polyelectrolytes dextran sulfate and poly-L-arginine were used for capsule shell assembly. The shell thickness-dependent encapsulation efficiency was measured by enzyme-linked immunosorbent assay. A maximum encapsulation efficiency of 42% could be achieved by microcapsules with a shell thickness of 14 layers. The effects of microcapsule concentration and shell thickness on cytotoxicity, FGF2 release kinetics, and L929 cell proliferation were evaluated in vitro. The advantage of using microcapsules as the carrier for FGF2 controlled release for enhancing L929 cell proliferation was analyzed.
Department of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore 117574, Singapore.
Zhen
Z
She
Chunxia
C
Wang
Jun
J
Li
Gleb B
GB
Sukhorukov
Maria N
MN
Antipina
D000818:Animals; D002119:Calcium Carbonate / Q000737:chemistry; D002214:Capsules; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016264:Dextran Sulfate / Q000737:chemistry* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004339:Drug Compounding; D004355:Drug Stability; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D018613:Microscopy, Confocal; D010455:Peptides / Q000737:chemistry* / Q000633:toxicity
D002214:Capsules; D010455:Peptides; D016222:Fibroblast Growth Factor 2; C015462:polyarginine; D016264:Dextran Sulfate; D002119:Calcium Carbonate