Comparison of a retrotransposon-based marker with microsatellite markers  for discriminating accessions of Vitis vinifera.
Identification and knowledge concerning genetic  diversity are fundamental for efficient management and use of grapevine  germplasm. Recently, new types of molecular markers have been developed, such  as retrotransposon-based markers. Because of their multilocus pattern,  retrotransposon-based markers might be able to differentiate grapevine accessions  with just one pair of primers. In order to evaluate the efficiency of this type  of marker, we compared retrotransposon marker Tvv1 with seven  microsatellite markers frequently used for genotyping of the genus Vitis (VVMD7,  VVMD25, VVMD5, VVMD27, VVMD31, VVS2, and VZAG62). The reference population that  we used consisted of 26 accessions of Vitis, including seven European  varieties of Vitis vinifera, four North American varieties and hybrids  of Vitis labrusca, and 15 rootstock hybrids obtained from crosses of  several Vitis species. Individually, the Tvv1 and the group of  seven SSR markers were capable of distinguishing all accessions except 'White  Niagara' compared to 'Red Niagara'. Using the Structure software, the  retrotransposon marker Tvv1 generated two clusters: one with V.  vinifera plus North American varieties and the other comprising rootstocks.  The seven SSR markers generated five clusters: V. vinifera, the North  American varieties, and three groups of rootstock hybrids. The percentages of  variation explained by the first two components in the principal coordinate  analysis were 65.21 (Tvv1) and 50.42 (SSR markers) while the Mantel  correlation between the distance matrixes generated by the two types of markers  was 42.5%. We conclude that the Tvv1 marker is useful for DNA  fingerprinting, but it lacks efficiency for discrimination of structured  groups.
Departamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
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Sant'Ana
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JL
Ferreira
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Rocha
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Borém
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Pasqual
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Cançado
D018744:DNA, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D018626:Retroelements / Q000235:genetics*; D027843:Vitis / Q000145:classification / Q000235:genetics*
D018744:DNA, Plant; D018626:Retroelements