The interaction pattern of murine serum ficolin-A with microorganisms.
The ficolins are soluble pattern recognition molecules in the lectin pathway of complement, but the spectrum and mode of interaction with pathogens are largely unknown. In this study, we investigated the binding properties of the murine serum ficolin-A towards a panel of different clinical relevant microorganisms (N = 45) and compared the binding profile with human serum ficolin-2 and ficolin-3. Ficolin-A was able to bind Gram-positive bacteria strains including E. faecalis, L. monocytogenes and some S. aureus strains, but not to the investigated S. agalactiae (Group B streptococcus) strains. Regarding Gram-negative bacteria ficolin-A was able to bind to some E. coli and P. aeruginosa strains, but not to the investigated Salmonella strains. Of particular interest ficolin-A bound strongly to the pathogenic E. coli, O157:H7 and O149 strains, but it did not bind to the non-pathogenic E. coli, ATCC 25922 strain. Additionally, ficolin-A was able to bind purified LPS from these pathogenic strains. Furthermore, ficolin-A bound to a clinical isolate of the fungus A. fumigatus. In general ficolin-2 showed similar selective binding spectrum towards pathogenic microorganisms as observed for ficolin-A indicating specific pathophysiological roles of these molecules in host defence. In contrast, ficolin-3 did not bind to any of the investigated microorganisms and the anti-microbial role of ficolin-3 still remains elusive.
Laboratory of Molecular Medicine, Department of Clinical Immunology, Rigshospitalet, Copenhagen, Denmark.
Tina
T
Hummelshøj
Ying Jie
YJ
Ma
Lea
L
Munthe-Fog
Thomas
T
Bjarnsholt
Claus
C
Moser
Mikkel-Ole
MO
Skjoedt
Luigina
L
Romani
Teizo
T
Fujita
Yuichi
Y
Endo
Peter
P
Garred
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D005658:Fungi / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000097:blood* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D000097604:Ficolins
D037102:Lectins; D008070:Lipopolysaccharides; D000097604:Ficolins