In vitro and in vivo anthelmintic activity of Euphorbia helioscopia L.
The anthelmintic potentials of the aqueous and methanol extracts of Euphorbia helioscopia were investigated. In folklore medicine, E. helioscopia (Euphorbiaceae) is used in the treatment of various gastrointestinal ailments and diseases. A worm motility inhibition (WMI) assay and egg hatch assay (EHA) were used for in vitro assessment, and a faecal egg count reduction (FECR) assay was used for an in vivo study. The in vitro study revealed anthelmintic effects of crude methanolic extracts of E. helioscopia on live Haemonchus contortus worms as evident from their paralysis and/or death at 8h after exposure, different concentrations (12.5 mg ml(-1), 25 mg ml(-1) and 50 mg ml(-1)) of aqueous and methanolic extracts were used against H. contortus which exhibited dose-dependent anthelmintic effects on H. contortus. Aqueous and methanolic extracts of aerial parts (stem, leaves and flowers) of E. helioscopia were found to have very low percent inhibitory egg hatching as compared to levamisole. It is concluded that the entire plant of E. helioscopia possesses significant anthelmintic activity and could be a potential alternative for treating cases of helminth infections in ruminants.
Centre of Research for Development, University of Kashmir, Srinagar 190 006, India. bashir.lone@gmail.com
Bashir A
BA
Lone
M Z
MZ
Chishti
Fayaz Ahmad
FA
Bhat
Hidayatullah
H
Tak
Suhaib A
SA
Bandh
D000818:Animals; D000871:Anthelmintics / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D028481:Euphorbia / Q000737:chemistry*; D005243:Feces / Q000469:parasitology; D005260:Female; D008297:Male; D010270:Parasite Egg Count; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy / Q000469:parasitology*
D000871:Anthelmintics; D010936:Plant Extracts