Spectrofluorimetric determination of human serum albumin using terbium-danofloxacin probe.
A spectrofluorimetric method is proposed for the determination of human serum albumin (HSA) and bovine serum albumin (BSA) using terbium-danofloxacin (Tb(3+)-Dano) as a fluorescent probe. These proteins remarkably enhance the fluorescence intensity of the Tb(3+)-Dano complex at 545 nm, and the enhanced fluorescence intensity of Tb(3+)-Dano is proportional to the concentration of proteins (HSA and BSA). Optimum conditions for the determination of HSA were investigated and found that the maximum response was observed at: pH = 7.8, [Tb(3+)] = 8.5 × 10(-5) mol L(-1), [Dano] = 1.5 × 10(-4) mol L(-1). The calibration graphs for standard solutions of BSA, HSA, and plasma samples of HSA were linear in the range of 0.2 × 10(-6) - 1.3 × 10(-6) mol L(-1), 0.2 × 10(-6) - 1.4 × 10(-6) mol L(-1), and 0.2 × 10(-6) - 1 × 10(-6) mol L(-1), respectively. The detection limits (S/N = 3) for BSA, HSA, and plasma sample of HSA were 8.7 × 10(-8) mol L(-1), 6.2 × 10(-8) mol L(-1), and 8.1 × 10(-8) mol L(-1), respectively. The applicability of the method was checked using a number of real biological plasma samples and was compared with the UV spectrometric reference method. The results was showed that the method could be regarded as a simple, practical, and sensitive alternative method for determination of albumin in biological samples.
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Amir M
AM
Ramezani
Jamshid L
JL
Manzoori
Mohammad
M
Amjadi
Abolghasem
A
Jouyban
D000818:Animals; D002138:Calibration; D002417:Cattle; D024841:Fluoroquinolones / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D013725:Terbium / Q000737:chemistry*; D014325:Tromethamine / Q000737:chemistry
D024841:Fluoroquinolones; D012709:Serum Albumin; D012997:Solvents; D013501:Surface-Active Agents; D014325:Tromethamine; D013725:Terbium; C068581:danofloxacin