Plasma protein adsorption to zwitterionic poly (carboxybetaine methacrylate) modified surfaces: chain chemistry and end-group effects on protein adsorption kinetics, adsorbed amounts and immunoblots.
Protein-surface interactions are crucial to the overall biocompatability of biomaterials, and are thought to be the impetus towards the adverse host responses such as blood coagulation and complement activation. Only a few studies hint at the ultra-low fouling potential of zwitterionic poly(carboxybetaine methacrylate) (PCBMA) grafted surfaces and, of those, very few systematically investigate their non-fouling behavior. In this work, single protein adsorption studies as well as protein adsorption from complex solutions (i.e. human plasma) were used to evaluate the non-fouling potential of PCBMA grafted silica wafers prepared by nitroxide-mediated free radical polymerization. PCBMAs used for surface grafting varied in charge separating spacer groups that influence the overall surface charges, and chain end-groups that influence the overall hydrophilicity, thereby, allows a better understanding of these effects towards the protein adsorption for these materials. In situ ellipsometry was used to quantify the adsorbed layer thickness and adsorption kinetics for the adsorption of four proteins from single protein buffer solutions, viz, lysozyme, α-lactalbumin, human serum albumin and fibrinogen. Total amount of protein adsorbed on surfaces differed as a function of surface properties and protein characteristics. Finally, immunoblots results showed that human plasma protein adsorption to these surfaces resulted, primarily, in the adsorption of human serum albumin, with total protein adsorbed amounts being the lowest for PCBMA-3 (TEMPO). It was apparent that surface charge and chain hydrophilicity directly influenced protein adsorption behavior of PCBMA systems and are promising materials for biomedical applications.
Chemical and Materials Engineering Department, University of Alberta, Edmonton, AB, Canada.
Sinoj
S
Abraham
Markian S
MS
Bahniuk
Larry D
LD
Unsworth
D000327:Adsorption; D001622:Betaine / Q000737:chemistry*; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007700:Kinetics; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties
D001798:Blood Proteins; D011109:Polymethacrylic Acids; C517178:polycarboxybetaine methacrylate; D001622:Betaine