CCS52 and DEL1 genes are key components of the endocycle in nematode-induced feeding sites.
The establishment of galls and syncytia as feeding sites induced by root-knot and cyst nematodes, respectively, involves a progressive increase in nuclear and cellular size. Here we describe the functional characterization of endocycle activators CCS52A, CCS52B and a repressor of the endocycle, DEL1, during two types of nematode feeding site development in Arabidopsis thaliana. In situ hybridization analysis showed that expression of CCS52A1 and CCS52B was strongly induced in galls and syncytia and DEL1 was stably but weakly expressed throughout feeding site development. Down-regulation and over-expression of CCS52 and DEL1 in Arabidopsis drastically affected giant cell and syncytium growth, resulting in restrained nematode development, illustrating the need for mitotic activity and endo-reduplication for feeding site maturation. Exploiting the mechanism of endo-reduplication may be envisaged as a strategy to control plant-parasitic nematodes.
Institut National de la Recherche Agronomique, UMR 1355 ISA/Centre National de la Recherche Scientifique, UMR 7254 ISA/Université de Nice-Sophia Antipolis, UMR ISA, 400 Route des Chappes, Sophia-Antipolis, France. janice.almeida-engler@sophia.inra.fr
Janice
J
de Almeida Engler
Tina
T
Kyndt
Paulo
P
Vieira
Elke
E
Van Cappelle
Veronique
V
Boudolf
Vanesa
V
Sanchez
Carolina
C
Escobar
Lieven
L
De Veylder
Gilbert
G
Engler
Pierre
P
Abad
Godelieve
G
Gheysen
D000818:Animals; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D062951:Endoreduplication; D005260:Female; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D055786:Gene Knockout Techniques; D015726:Giant Cells / Q000378:metabolism / Q000469:parasitology; D010935:Plant Diseases / Q000469:parasitology*; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000469:parasitology; D030821:Plants, Genetically Modified; D011003:Ploidies; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000469:parasitology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014431:Tylenchoidea / Q000166:cytology / Q000502:physiology*
D029681:Arabidopsis Proteins; C583229:CCS52B protein, Arabidopsis; D018797:Cell Cycle Proteins; C500416:DEL1 protein, Arabidopsis; C540491:FZR2 protein, Arabidopsis; D014157:Transcription Factors