Up-regulation of blood arachidonate (20:4) levels in patients with chronic obstructive pulmonary disease.
OBJECTIVE
Plasma arachidonate (20:4) levels in patients with chronic obstructive pulmonary disease (COPD) were investigated.


METHODS
Plasma was extracted and free fatty acids (FFAs) were separated using column chromatography and measured by fluorescence. Plasma 20:4 levels and its percentage relative to total FFA levels (%20:4) were measured in COPD (n = 18) and control (n = 20) subjects.


CONCLUSIONS
FFA levels were lower in COPD compared with normals. However, there was a significant increase in %20:4 levels in COPD patients (GOLD stage I/II 0.9 ± 0.4%; GOLD stage III/IV 1.1 ± 0.1%) compared with control subjects (0.6 ± 0.1, p < 0.05). %20:4 is a potential biomarker for COPD.
Department of Respiratory Medicine, Kyorin University School of Medicine, Tokyo, Japan. wadagh@ks.kyorin-u.ac.jp
Hiroo
H
Wada
Shin-ichi
S
Hagiwara
Erika
E
Saitoh
Ryuji
R
Ieki
Yorihiro
Y
Yamamoto
Ian M
IM
Adcock
Hajime
H
Goto
D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D001095:Arachidonic Acids / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000503:physiopathology; D012044:Regression Analysis; D018709:Statistics, Nonparametric; D015854:Up-Regulation; D014797:Vital Capacity
D001095:Arachidonic Acids; D015415:Biomarkers; D005230:Fatty Acids, Nonesterified