Serologic study of pig-associated viral zoonoses in Laos.
We conducted a serologic survey of four high-priority pig-associated viral zoonoses, Japanese encephalitis virus (JEV), hepatitis E virus (HEV), Nipah virus (NiV), and swine influenza virus (SIV), in Laos. We collected blood from pigs at slaughter during May 2008-January 2009 in four northern provinces. Japanese encephalitis virus hemagglutination inhibition seroprevalence was 74.7% (95% confidence interval [CI] = 71.5-77.9%), JEV IgM seroprevalence was 2.3% (95% CI = 1.2-3.2%), and HEV seroprevalence was 21.1% (95% CI = 18.1-24.0%). Antibodies to SIV were detected in 1.8% (95% CI = 0.8-2.8%) of pigs by screening enzyme-linked immunosorbent assay, and only subtype H3N2 was detected by hemagglutination inhibition in two animals with an inconclusive enzyme-linked immunosorbent assay result. No NiV antibody-positive pigs were detected. Our evidence indicates that peak JEV and HEV transmission coincides with the start of the monsoonal wet season and poses the greatest risk for human infection.
James V
JV
Conlan
Khamphouth
K
Vongxay
Richard G
RG
Jarman
Robert V
RV
Gibbons
Ross A
RA
Lunt
Stanley
S
Fenwick
R C Andrew
RCA
Thompson
Stuart D
SD
Blacksell
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004664:Encephalitis Virus, Japanese / Q000302:isolation & purification / Q000472:pathogenicity; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D016752:Hepatitis E virus / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification / Q000472:pathogenicity; D007812:Laos / Q000453:epidemiology; D045405:Nipah Virus / Q000302:isolation & purification / Q000472:pathogenicity; D012307:Risk Factors; D016036:Seroepidemiologic Studies; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000097:blood / Q000453:epidemiology* / Q000635:transmission* / Q000821:virology; D015047:Zoonoses / Q000821:virology*
D000914:Antibodies, Viral; D007075:Immunoglobulin M