Cadmium tolerance in six poplar species.
Selection of poplar species with greater Cd tolerance and exploiting the physiological mechanisms involved in Cd tolerance are crucial for application of these species to phyto-remediation. The aim of this study is to investigate variation in Cd tolerance among the six poplar species and its underlying physiological mechanisms. Cuttings of six Populus species were cultivated for 10 weeks before exposure to either 0 or 200 μM CdSO(4) for 20 days. Gas exchange in mature leaves was determined by a portable photosynthesis system. Cd concentrations in tissues were analyzed by a flame atomic absorbance spectrometry. Subsequently, Cd amount per plant, bio-concentration factor (BCF) and translocation factor (T (f)) were calculated. Nonenzymatic compounds and activities of antioxidative enzymes in tissues were analyzed spectrophotometrically. Cd exposure caused decline in photosynthesis in four poplar species including Populus cathayana (zhonghua 1). Among the six species, P. cathayana (zhonghua 1) displayed the highest Cd concentrations in tissues, the largest Cd amount in aerial parts, the highest BCF in aerial parts and T (f) under Cd exposure. Under Cd stress, increases in total soluble sugars in roots but decreases in starch in roots, wood, and leaves of P. cathayana (zhonghua 1) were found. Induced O (2) (•-) and H(2)O(2) production in roots and leaves, and increases in free proline, soluble phenolics, and activities of antioxidative enzymes were observed in P. cathayana (zhonghua 1). Based on results of this pot experiment, it is concluded that P. cathayana (zhonghua 1) is superior to other five species for Cd phyto-remediation, and its well-coordinated physiological changes under Cd exposure confer the great Cd tolerance of this species.
College of Life Sciences, Northwest Agriculture & Forestry University, Yangling, Shaanxi 712100, People's Republic of China.
Jiali
J
He
Chaofeng
C
Ma
Yonglu
Y
Ma
Hong
H
Li
Jingquan
J
Kang
Tongxian
T
Liu
Andrea
A
Polle
Changhui
C
Peng
Zhi-Bin
ZB
Luo
D000222:Adaptation, Physiological; D002104:Cadmium / Q000633:toxicity*; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D032107:Populus / Q000187:drug effects / Q000502:physiology*; D012989:Soil Pollutants / Q000633:toxicity*
D012989:Soil Pollutants; D002104:Cadmium