Response of osteoclasts to titanium surfaces with increasing surface roughness: an in vitro study.
Osteoclasts are responsible for bone resorption and implant surface roughness promotes osseointegration. However, little is known about the effect of roughness on osteoclast activity. This study aims at the characterization of osteoclastic response to surface roughness. The number of osteoclasts, the tartrate-resistant acid phosphatase and matrix metalloproteinase (MMP) activities, the cell morphology and the actin-ring formation were examined on smooth (TS), acid-etched (TA) and sandblasted acid-etched (TLA) titanium and on native bone. Cell morphology was comparable on TA, TLA and bone, actin rings being similar in size on TLA and bone, but smaller on TA and virtually absent on TS. Gelatin zymography revealed increased proMMP-9 expression on TA, TLA, and bone compared to TS. In general, osteoclasts show similar characteristics on rough titanium surfaces and on bone, but reduced activity on smooth titanium surfaces. These results offer some insight into the involvement of osteoclasts in remodeling processes around implant surfaces.
Cells and BioMaterials, Department of Materials, ETH Zurich, Wolfgang-Pauli-Strasse 10, 8093, Zürich, Switzerland. j.brinkmann@utwente.nl
Jenny
J
Brinkmann
Thomas
T
Hefti
Falko
F
Schlottig
Nicholas D
ND
Spencer
Heike
H
Hall
D000135:Acid Phosphatase / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D048430:Cell Shape; D007527:Isoenzymes / Q000378:metabolism*; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000201:enzymology* / Q000502:physiology*; D013499:Surface Properties; D000071681:Tartrate-Resistant Acid Phosphatase; D014025:Titanium / Q000737:chemistry*
D000199:Actins; D007527:Isoenzymes; D014025:Titanium; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D020782:Matrix Metalloproteinases