Production, characterization and application of inulinase from fungal endophyte CCMB 328.
Inulinase (β-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v).
Laboratório de Enzimologia e Tecnologia de Fermentação, Departamento de Saúde, Universidade Estadual de Feira de Santana, BA, Brasil.
Diego S
DS
Nascimento
Gildomar
G
Valasques Junior
Pedro
P
Fernandes
Geise C A
GC
Ribeiro
Danyo M
DM
Lima
Aristóteles
A
Góes-Neto
Rodrigo Q
RQ
Oliveira
Rita de Cassia L
Rde C
Figueiredo-Ribeiro
Sandra A de
SA
Assis
D001938:Brazil; D003889:Desert Climate; D005658:Fungi / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000737:chemistry
D006026:Glycoside Hydrolases; C023086:inulinase