Recombinant ELISA using baculovirus-expressed VP2 for detection of antibodies against canine parvovirus.
The gene encoding the VP2 protein of canine parvovirus type 2 was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination, Western blotting and hemagglutination inhibition test, using Canine parvovirus type-2 (CPV-2) positive sera. An enzyme-linked immunosorbent assay (ELISA) using the rVP2 was used for testing CPV-2 positive and negative sera from dogs and for determining the threshold of maternally derived antibodies interfering with successful vaccination of pups against CPV-2.
Department of Veterinary Public Health, University of Bari, Valenzano (BA), Italy. g.elia@veterinaria.uniba.it
Gabriella
G
Elia
Costantina
C
Desario
Giulia
G
Pezzoni
Michele
M
Camero
Emiliana
E
Brocchi
Nicola
N
Decaro
Vito
V
Martella
Canio
C
Buonavoglia
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D002460:Cell Line; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015870:Gene Expression; D005822:Genetic Vectors; D007313:Insecta; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D017993:Parvovirus, Canine / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics; D014730:Veterinary Medicine / Q000379:methods*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins