Impact of cell division on intracellular uptake and nuclear targeting with fluorescent SiC-based nanoparticles.
Semiconductor nanoparticles (NPs) became important and wide-used tool for cell imaging because of their unique remarkable properties. Nevertheless, all previous investigations in this area were done on proliferating cells. For the first time, this work demonstrates strong influence of cell active proliferation/contact inhibition of proliferation on uptake of NPs. In addition, we show that cell division plays key-role in penetration of silicon carbide based NPs (SiC NPs) inside the cell nucleus. This may very likely concern other types of NPs able to reach the cell nuclei. In particular, observed effect of cell division gives perspectives for future selective cancer treatment with NPs.
University of Lyon, CarMeN Laboratory, UMR INSERM 1060, INSA de Lyon, University of Lyon, France. tetiana.serdiuk@gmail.com
Tetiana
T
Serdiuk
Vladimir
V
Lysenko
Barbara
B
Mognetti
Valery
V
Skryshevsky
Alain
A
Géloën
D041721:3T3-L1 Cells; D000818:Animals; D001692:Biological Transport; D017554:Carbon Compounds, Inorganic / Q000737:chemistry* / Q000378:metabolism*; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D053758:Nanoparticles; D012666:Semiconductors; D017655:Silicon Compounds / Q000737:chemistry* / Q000378:metabolism*
D017554:Carbon Compounds, Inorganic; D005456:Fluorescent Dyes; D017655:Silicon Compounds; C022088:silicon carbide