Contact chemoreceptors mediate male-male repulsion and male-female attraction during Drosophila courtship.
The elaborate courtship ritual of Drosophila males is dictated by neural circuitry established by the transcription factor Fruitless and triggered by sex-specific sensory cues. Deciphering the role of different stimuli in driving courtship behavior has been limited by the inability to selectively target appropriate sensory classes. Here, we identify two ion channel genes belonging to the degenerin/epithelial sodium channel/pickpocket (ppk) family, ppk23 and ppk29, which are expressed in fruitless-positive neurons on the legs and are essential for courtship. Gene loss-of-function, cell-inactivation, and cell-activation experiments demonstrate that these genes and neurons are necessary and sufficient to inhibit courtship toward males and promote courtship toward females. Moreover, these cells respond to cuticular hydrocarbons, with different cells selectively responding to male or female pheromones. These studies identify a large population of pheromone-sensing neurons and demonstrate the essential role of contact chemosensation in the early courtship steps of mate selection and courtship initiation.
Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute, 16 Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA.
Robert
R
Thistle
Peter
P
Cameron
Azeen
A
Ghorayshi
Lisa
L
Dennison
Kristin
K
Scott
D000818:Animals; D030801:Animals, Genetically Modified; D003380:Courtship; D004330:Drosophila / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism; D005260:Female; D007473:Ion Channels / Q000378:metabolism; D008297:Male; D052586:Mating Preference, Animal; D009474:Neurons / Q000378:metabolism; D012724:Sex Attractants / Q000378:metabolism*
D029721:Drosophila Proteins; D007473:Ion Channels; C573945:PPK23 protein, Drosophila; C573946:PPK29 protein, Drosophila; D012724:Sex Attractants