Direct scaffolding of biomimetic hydroxyapatite-gelatin nanocomposites using aminosilane cross-linker for bone regeneration.
Hydroxyapatite-gelatin modified siloxane (GEMOSIL) nanocomposite was developed by coating, kneading and hardening processes to provide formable scaffolding for alloplastic graft applications. The present study aims to characterize scaffolding formability and mechanical properties of GEMOSIL, and to test the in vitro and in vivo biocompatibility of GEMOSIL. Buffer Solution initiated formable paste followed by the sol-gel reaction led to a final hardened composite. Results showed the adequate coating of aminosilane, 11-19 wt%, affected the cohesiveness of the powders and the final compressive strength (69 MPa) of the composite. TGA and TEM results showed the effective aminosilane coating that preserves hydroxyapatite-gelatin nanocrystals from damage. Both GEMOSIL with and without titania increased the mineralization of preosteoblasts in vitro. Only did titania additives revealed good in vivo bone formation in rat calvarium defects. The scaffolding formability, due to cohesive bonding among GEMOSIL particles, could be further refined to fulfill the complicated scaffold processes.
Department of Materials Science and Engineering, North Carolina State University, Raleigh, NC 27606, USA.
Chi-Kai
CK
Chiu
Joao
J
Ferreira
Tzy-Jiun M
TJ
Luo
Haixia
H
Geng
Feng-Chang
FC
Lin
Ching-Chang
CC
Ko
D000818:Animals; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000737:chemistry; D001843:Bone Cements / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012821:Silanes / Q000494:pharmacology*; D012886:Skull / Q000293:injuries / Q000473:pathology; D012887:Skull Fractures / Q000628:therapy; D054457:Tissue Scaffolds / Q000737:chemistry*
D001843:Bone Cements; D003432:Cross-Linking Reagents; D012821:Silanes; D005780:Gelatin; D017886:Durapatite