XRCC1 Arg399Gln was associated with repair capacity for DNA damage induced by occupational chromium exposure.
BACKGROUND
Occupational chromium exposure may induce DNA damage and lead to lung cancer and other work-related diseases. DNA repair gene polymorphisms, which may alter the efficiency of DNA repair, thus may contribute to genetic susceptibility of DNA damage. The aim of this study was to test the hypothesis that the genetic variations of 9 major DNA repair genes could modulate the hexavalent chromium (Cr (VI))-induced DNA damage.


RESULTS
The median (P25-P75) of Olive tail moment was 0.93 (0.58-1.79) for individuals carrying GG genotype of XRCC1 Arg399Gln (G/A), 0.73 (0.46-1.35) for GA heterozygote and 0.50 (0.43-0.93) for AA genotype. Significant difference was found among the subjects with three different genotypes (P = 0.048) after adjusting the confounding factors. The median of Olive tail moment of the subjects carrying A allele (the genotypes of AA and GA) was 0.66 (0.44-1.31), which was significantly lower than that of subjects with GG genotype (P = 0.043). The A allele conferred a significantly reduced risk of DNA damage with the OR of 0.39 (95% CI: 0.15-0.99, P = 0.048). No significant association was found between the XRCC1Arg194Trp, ERCC1 C8092A, ERCC5 His1104Asp, ERCC6 Gly399Asp, GSTP1 Ile105Val, OGG1 Ser326Cys, XPC Lys939Gln, XPD Lys751Gln and DNA damage.


CONCLUSIONS
The polymorphism of Arg399Gln in XRCC1 was associated with the Cr (VI)- induced DNA damage. XRCC1 Arg399Gln may serve as a genetic biomarker of susceptibility for Cr (VI)- induced DNA damage.
Department of Epidemiology and Biostatistics, Zhejiang University School of Medicine, 388 Yu-Hang-Tang Road, Hangzhou 310058, Zhejiang, People's Republic of China.
Xuhui
X
Zhang
Xuan
X
Zhang
Lei
L
Zhang
Qing
Q
Chen
Zhangping
Z
Yang
Jingmin
J
Yu
Hong
H
Fu
Yimin
Y
Zhu
D001120:Arginine / Q000235:genetics*; D001483:Base Sequence; D002857:Chromium / Q000633:toxicity*; D015986:Confounding Factors, Epidemiologic; D004249:DNA Damage; D017931:DNA Primers; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D005998:Glycine / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D016273:Occupational Exposure; D016133:Polymerase Chain Reaction; D000076105:X-ray Repair Cross Complementing Protein 1
D017931:DNA Primers; D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; D002857:Chromium; D001120:Arginine; D005998:Glycine