Diffusion tensor MRI to assess skeletal muscle disruption following eccentric exercise.
BACKGROUND
Structural evidence of exercise-induced muscle disruption has traditionally involved histological analysis of muscle tissue obtained by needle biopsy, however, there are multiple limitations with this technique. Recently, diffusion tensor magnetic resonance imaging (DT-MRI) has been successfully demonstrated to noninvasively assess skeletal muscle abnormalities induced by traumatic injury.


METHODS
To determine the potential for DT-MRI to detect musculoskeletal changes after a bout of eccentric exercise, 10 healthy men performed 300 eccentric actions on an isokinetic dynamometer. DT-MRI measurements and muscle biopsies from the vastus lateralis were obtained before and 24 h post-exercise.


RESULTS
Z-band streaming was higher 24 h post-exercise compared with baseline (P < 0.05). The histological indices of damage coincided with changes in DT-MRI parameters of fractional anisotropy (FA) and apparent diffusion coefficient; reflecting altered skeletal muscle geometry (P < 0.05). Z-band streaming quantified per fiber correlated with FA (r = -0.512; P < 0.05).


CONCLUSIONS
DT-MRI can detect changes in human skeletal muscle structure following eccentric exercise.
Exercise Metabolism Research Group, Department of Kinesiology, McMaster University, Hamilton, Ontario.
Naomi M
NM
Cermak
Michael D
MD
Noseworthy
Jacqueline M
JM
Bourgeois
Mark A
MA
Tarnopolsky
Martin J
MJ
Gibala
D000328:Adult; D056324:Diffusion Tensor Imaging; D015444:Exercise / Q000502:physiology*; D006801:Humans; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D018482:Muscle, Skeletal / Q000293:injuries / Q000473:pathology* / Q000502:physiology