Mincle polarizes human monocyte and neutrophil responses to Candida albicans.
The distribution and function of the C-type lectin Mincle has not previously been investigated in human cells, although mouse models have demonstrated a non-redundant role for Mincle in the host response to fungal infections. This study identified an unusual pattern of reciprocal expression of Mincle on peripheral blood monocytes or neutrophils isolated from the same donor. Expression on monocytes was inversely correlated with phagocytosis and yeast killing, but was necessary for the induction of inflammatory cytokines in response to ex vivo Candida challenge. In contrast, Mincle expression on neutrophils was associated with phagocytic and candidacidal potential of those cells. Candida challenge upregulated Mincle expression but only in Mincle+ cells. These data highlight species-specific differences between the regulation of Mincle expression in mouse and man. Reciprocal expression of Mincle modified the candidacidal potential of monocytes or neutrophils, suggesting it may also polarize the type of host response to fungal infection.
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland, Australia.
Dipti
D
Vijayan
Kristen J
KJ
Radford
Anthony G
AG
Beckhouse
Robert B
RB
Ashman
Christine A
CA
Wells
D002176:Candida albicans / Q000276:immunology* / Q000502:physiology; D016764:Cell Polarity / Q000276:immunology; D002478:Cells, Cultured; D005434:Flow Cytometry; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D053583:Interleukin-1beta / Q000276:immunology / Q000378:metabolism; D037181:Lectins, C-Type / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D009000:Monocytes / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis / Q000276:immunology; D011971:Receptors, Immunologic / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism
C482593:CLEC4D protein, human; D053583:Interleukin-1beta; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D011971:Receptors, Immunologic; D014409:Tumor Necrosis Factor-alpha