Microglia/monocytes with NG2 expression have no phagocytic function in the cortex after LPS focal injection into the rat brain.
While OX42(+) microglia/macrophages have been considered as a scavenger in the brain, NG2(+) cells are generally considered as oligodendrocyte progenitor cells or function-unknown glial cells. Recent evidence showed that under some pathological conditions, certain cells have become positive for both anti-NG2 and anti-OX42 antibodies. Our results suggested that some OX42(+) microglia or macrophages were induced to express NG2 proteins 3 and 5 days later after focal injection of lipopolysaccharide into the brain cortex of Sprague-Dawley rats. In consideration of the induction of NG2 expression may associate with gaining or losing functions of microglia/macrophages, we further showed that, while OX42(+) or ED1(+) microglia/macrophages presented active phagocytic function, NG2(+) /OX42(+) cells failed to engulf latex beads. The induced expression of NG2 protein may possibly indicate the functional diversity of activated microglia/macrophages in the brain.
Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Lie
L
Zhu
Ping
P
Xiang
Kun
K
Guo
Anni
A
Wang
Jia
J
Lu
Samuel Sam Wah
SS
Tay
Hua
H
Jiang
Bei Ping
BP
He
D000818:Animals; D000941:Antigens / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000099216:Chondroitin Sulfate Proteoglycan 4
D000941:Antigens; D008070:Lipopolysaccharides; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4