[The effectiveness of hand hygiene products on MRSA colonization of health care workers by using CHROMagar MRSA].
The aims of this study were; to investigate the hand hygiene compliance of the health care workers (HCWs) during their routine patient care, to determine the methicillin-resistant Staphylococcus aureus (MRSA) hand colonization of the HCWs, to investigate the effect of different hand hygiene products on MRSA colonization and to evaluate the effectiveness of chromogenic agar for detecting MRSA. HCWs were investigated during their routine patient care and hand cultures were taken before and after hand wash/hygiene. Two different techniques were used to obtain the hand cultures: fingertip method (CHROMagar MRSA containing HygiSlide); and direct swab method and then inoculation to CHROMagar MRSA media. MRSA strains grown on those cultures were confirmed with conventional methods. A total of 100 HCWs (of them 61 were female; mean age: 32.7 ± 5.2 years; age range: 25-51 years) involving physicians (n= 33), nurses (n= 38) and health care assistants (n= 29), were included in the study. MRSA was detected in 39% and 11% before hand hygiene and in 13% and 6% after hand hygiene, with HygiSlide CHROMagar media and with CHROMagar in plate media, respectively. No difference were found regarding clinics, occupations, or the type of patient handling in those HCWs who were positive (n= 13) for MRSA colonization following hand hygiene, and those who were negative (n= 26). However, the type of the hand hygiene product used exhibited a statistical difference. None of the seven HCWs who used alcohol based hand rub revealed growth in the second culture while 10 of 19 (53%) HCWs who used soap and three of 13 (23%) HCWs who used chlorhexidine were still colonized with MRSA. In terms of reduction in the MRSA counts, the most effective one was the alcohol based hand rub while the soap was the least, since seven of 19 (37%) HCWs who used soap showed no reduction at all in the MRSA counts. A high ratio of hand colonization with MRSA was detected in our hospital staff (39%). It was shown that the colonization could be reduced significantly (with a rate of 66%) with hand hygiene. Alcohol based hand rub was found to be the most effective method in hand hygiene. The fingertip technique was found to be superior to inoculation to plate media for obtaining hand cultures and CHROMagar MRSA media was found to be rapid, effective and practical for detecting the MRSA hand colonization.
Ankara Training and Research Hospital, Clinic of Infectious Diseases and Clinical Microbiology, Ankara, Turkey. drztufan@yahoo.com
Zeliha
Z
Koçak Tufan
Hasan
H
Irmak
Cemal
C
Bulut
Salih
S
Cesur
Sami
S
Kınıklı
Ali Pekcan
AP
Demiröz
D000328:Adult; D002710:Chlorhexidine / Q000592:standards; D002863:Chromogenic Compounds / Q000592:standards; D003428:Cross Infection / Q000517:prevention & control; D003470:Culture Media / Q000592:standards; D004202:Disinfectants / Q000008:administration & dosage / Q000592:standards*; D000431:Ethanol / Q000592:standards; D005260:Female; D006225:Hand / Q000382:microbiology*; D006235:Hand Disinfection; D063373:Hand Hygiene / Q000379:methods* / Q000592:standards; D006282:Health Personnel; D006801:Humans; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000254:growth & development* / Q000302:isolation & purification; D008875:Middle Aged; D012915:Soaps / Q000592:standards; D013048:Specimen Handling / Q000379:methods / Q000592:standards; D013203:Staphylococcal Infections / Q000517:prevention & control*
D002863:Chromogenic Compounds; D003470:Culture Media; D004202:Disinfectants; D012915:Soaps; D000431:Ethanol; D002710:Chlorhexidine