Osteoprotegerin gene polymorphism is not associated with prosthetic joint infection after total joint arthroplasty in the Czech population.
OBJECTIVE
Osteoprotegerin (OPG; official gene symbol: TNFRSF11B) is considered a negative regulator of bone resorption via inhibition of osteoclast differentiation. Further, OPG expression has been detected in Prosthetic Joint Infection (PJI) a serious complication limiting the overall outcome of total joint arthroplasty (TJA). As OPG may be a candidate molecule for PJI pathogenesis, we investigated whether genetic variation in the OPG promoter, namely the SNP at position -163 was associated with PJI.


METHODS
OPG -163 T/C SNP (rs3102735) was genotyped by polymerase chain reaction with sequence specific primers (PCR-SSP) in 98 Czech patients with PJI and two Czech control groups: 1) aseptic TJA control [251 patients with TJA who did not develop PJI at least 6 yrs. after the surgery] and 2) population control (185 healthy control subjects without TJA).


RESULTS
The distribution of OPG -163 SNP genotypes complied with the Hardy-Weinberg equilibrium in all three groups. The allele frequencies of OPG -163 SNP were similar in patients with PJI (minor allele frequency: 0.14), those with aseptic TJA (0.13) and population controls (0.14, P>0.05). Further, there was no significant difference in genotype or phenotype frequency (carriage rate) between patients with PJI and both control groups (P>0.05).


CONCLUSIONS
In a Czech population, the OPG -163 T/C SNP has not been found to be associated with PJI.
Laboratory of Immunogenomics and Imunoproteomics, Department of Immunology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.
Zdenka
Z
Navratilova
Jiri
J
Gallo
Matej
M
Smizansky
Frantisek
F
Mrazek
Martin
M
Petrek
D000328:Adult; D000368:Aged; D019643:Arthroplasty, Replacement / Q000009:adverse effects*; D016022:Case-Control Studies; D018153:Czech Republic; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D053244:Osteoprotegerin / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D016459:Prosthesis-Related Infections / Q000235:genetics*; D055815:Young Adult
D053244:Osteoprotegerin