[Kiss-1 gene expression after radiation and its association with proliferation and apoptosis in colorectal cancer cells].
OBJECTIVE
To investigate the change of expression level of metastasis suppressor gene Kiss-1 in the colorectal cancer cell line SW480 after radiation, and to determine its association with the proliferation and apoptosis of SW480 cells.


METHODS
SW480 cells were divided into control group (0 Gy) and study groups (2, 4, 6, 8 Gy). Cells in the study groups were irradiated by 6-MV X-ray radiation for 48 hours. Immunohistochemistry and real-time PCR methods were used to investigate the influence of radiation on Kiss-1 gene expression of SW480. Colony formation assay was used to detect the proliferation of SW480. Flow cytometry-Annexin- V/PI assay was used to observe the change of the apoptosis rate.


RESULTS
Compared with the control group, Kiss-1 protein expression increased after radiation of 6, 8 Gy (P<0.05), but no significant changes were observed after radiation of 2, 4 Gy(P>0.05). Kiss-1 gene mRNA level increased after radiation of 2, 4, 6 Gy, while no obvious change was observed for 8 Gy radiation. The apoptosis rates increased for 4, 6, 8 Gy radiation(P<0.05), however, there was no significant difference for 2 Gy radiation (P<0.05).


CONCLUSIONS
Radiation may increase Kiss-1 gene expression in SW480 cells, which results in decreases proliferation and increases apoptosis in residual surviving cells.
Department of Gastrointestinal Surgery, Fujian Medical University, Fuzhou, China. chenshaoqin@medmail.com.cn
Shao-qin
SQ
Chen
Ming-mei
MM
Tu
Qi-bao
QB
Dai
Su-yong
SY
Lin
Chun-lin
CL
Ke
D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D059648:Kisspeptins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics; D014965:X-Rays
C106628:KISS1 protein, human; D059648:Kisspeptins; D012333:RNA, Messenger