Labelling of olfactory ensheathing cells with micron-sized particles of iron oxide and detection by MRI.
A crucial issue in transplant-mediated repair of the damaged central nervous system (CNS) is serial non-invasive imaging of the transplanted cells, which has led to interest in the application of magnetic resonance imaging (MRI) combined with designated intracellular magnetic labels for cell tracking. Micron-sized particles of iron oxide (MPIO) have been successfully used to track cells by MRI, yet there is relatively little known about either their suitability for efficient labelling of specific cell types, or their effects on cell viability. The purpose of this study was to develop a suitable MPIO labelling protocol for olfactory ensheathing cells (OECs), a type of glia used to promote the regeneration of CNS axons after transplantation into the injured CNS. Here, we demonstrate an OEC labelling efficiency of >90% with an MPIO incubation time as short as 6 h, enabling intracellular particle uptake for single-cell detection by MRI without affecting cell proliferation, migration and viability. Moreover, MPIO are resolvable in OECs transplanted into the vitreous body of adult rat eyes, providing the first detailed protocol for efficient and safe MPIO labelling of OECs for non-invasive MRI tracking of transplanted OECs in real time for use in studies of CNS repair and axon regeneration.
MI Lab and Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway. ioanna.sandvig@ntnu.no
Ioanna
I
Sandvig
Linh
L
Hoang
Thomas C P
TC
Sardella
Susan C
SC
Barnett
Christian
C
Brekken
Kåre
K
Tvedt
Martin
M
Berry
Olav
O
Haraldseth
Axel
A
Sandvig
Marte
M
Thuen
D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D005290:Ferric Compounds / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009830:Olfactory Bulb / Q000166:cytology*; D010316:Particle Size; D051381:Rats; D011916:Rats, Inbred F344; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors; D014048:Tolonium Chloride / Q000378:metabolism
D005290:Ferric Compounds; D014048:Tolonium Chloride; C000499:ferric oxide