Release profiles of tricalcium phosphate nanoparticles from poly(L-lactic acid) electrospun scaffolds with single component, core-sheath, or porous fiber morphologies: effects on hASC viability and osteogenic differentiation.
Functional PLA scaffolds are created with single component, core-sheath, or porous fiber morphology and doped with TCP nanoparticles to study the release profiles for use in bone tissue engineering applications. Pharmacokinetic analyses are performed for the three different nanofibrous structures after doping with TCP. Results indicate that single component and porous fiber scaffolds exhibit an initial-burst release profile whereas core-sheath fibers show a steady release. All scaffolds are then seeded with human adipose-derived stem cells (hASC), which remain viable and continue proliferation on all nanofibrous morphologies for up to 21 d. Osteogenic differentiation of hASC and cell-mediated calcium accretion are largest on porous fibers.
College of Textiles, North Carolina State University, Raleigh, NC 27695, USA.
Mahsa Mohiti
MM
Asli
Behnam
B
Pourdeyhimi
Elizabeth G
EG
Loboa
D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects; D000328:Adult; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); C018392:tricalcium phosphate