Using a nanopore for single molecule detection and single cell transfection.
We assert that it is possible to trap and identify proteins, and even (conceivably) manipulate proteins secreted from a single cell (i.e. the secretome) through transfection via electroporation by exploiting the exquisite control over the electrostatic potential available in a nanopore. These capabilities may be leveraged for single cell analysis and transfection with single molecule resolution, ultimately enabling a careful scrutiny of tissue heterogeneity.
University of Notre Dame, Notre Dame, IN 46556, USA.
Edward M
EM
Nelson
Volker
V
Kurz
Jiwook
J
Shim
Winston
W
Timp
Gregory
G
Timp
D045744:Cell Line, Tumor; D006801:Humans; D044085:Microfluidics; D049329:Nanostructures; D014162:Transfection