Isolation of mouse mammary epithelial subpopulations: a comparison of leading methods.
Isolation of mammary epithelial subpopulations, including stem and progenitor cells, has become a standard technique in recent years. However, a number of methods and approaches for this have developed and the relative benefits of the different approaches, and the reason for their development, have not always been clear. Here, three of the leading laboratories working on the separation of mammary cell subpopulations have summarised their methods, highlighted their differences and similarities and also discussed the reasoning behind the approaches they have taken. This article will assist workers establishing mammary cell separation protocols in their laboratories to make informed choices about the methods they should use.
European Cancer Stem Cell Research Institute, Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, UK. SmalleyMJ@Cardiff.ac.uk
Matthew J
MJ
Smalley
Howard
H
Kendrick
Julie M
JM
Sheridan
Joseph L
JL
Regan
Michael D
MD
Prater
Geoffrey J
GJ
Lindeman
Christine J
CJ
Watson
Jane E
JE
Visvader
John
J
Stingl
D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism*; D005260:Female; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D009940:Organoids / Q000166:cytology / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods
D000954:Antigens, Surface