Galectin-10 is released in the nasal lavage fluid of patients with aspirin-sensitive respiratory disease.
The aim of this work was to determine the presence of galectin-10 in nasal lavage fluid (NLF) of patients with aspirin-sensitive respiratory disease (ASRD) before and after challenge with L-ASA (aspirin) by ELISA. Fifteen ASRD patients, ten aspirin-tolerant asthmatics (ATA), and fifteen healthy controls (HC) were studied. The baseline presence of Galectin-10 in PBMC was determined using real time PCR. Galectin-10 was evaluated in tissue of nasal polyps by western blot. Our results showed a lower expression in PBMC of ASRD patients than in ATA and healthy controls. However, a higher concentration of galectin-10 in NLF was found in ASRD patients before and after L-ASA challenge; western blot confirmed a high expression of galectin-10 in tissue from nasal polyps obtained from ASRD patients. Our results suggest a probable role of galectin-10 in the inflammatory response observed in ASRD patients; however, confirmatory studies are needed.
Department of Immunogenetics and Allergy, Instituto Nacional de Enfermedades Respiratorias, Calzada Tlalpan 4502, 14080 Mexico, DF, Mexico. crisdanmara@yahoo.com
Ma Cristina
MC
Negrete-Garcia
Carla Yoneli
CY
Jiménez-Torres
Noe
N
Alvarado-Vásquez
A Rosalía
AR
Montes-Vizuet
J R
JR
Velázquez-Rodriguez
M Carmen
MC
Jimenez-Martinez
Luis Manuel
LM
Teran-Juárez
D000293:Adolescent; D000328:Adult; D000368:Aged; D001241:Aspirin / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D037161:Galectins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017817:Nasal Lavage Fluid / Q000737:chemistry*; D012140:Respiratory Tract Diseases / Q000139:chemically induced* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D037161:Galectins; D001241:Aspirin