Haplotype combination of the bovine INSIG1 gene sequence variants and association with growth traits in Nanyang cattle.
The insulin-induced gene 1 (INSIG1) gene encodes a protein that blocks proteolytic activation of sterol regulatory element binding proteins, which are transcription factors that activate genes that regulate cholesterol, fatty acid, and glucose metabolism. However, similar research for the bovine INSIG1 gene is lacking. Therefore, in this study, polymorphisms of the bovine INSIG1 gene were detected in 643 individuals from four cattle breeds by DNA pooling, forced PCR-RFLP, PCR-SSCP, and DNA sequencing methods. Only 10 novel SNPs were identified, which included four mutations in the coding region and the others in the introns. In Nanyang individuals, seven common haplotypes were identified based on four coding region SNPs. The haplotype GACT, with a frequency of 75.4%, was the most prevalent haplotypes and SNPs formed two linkage disequilibrium blocks with strong multi-allelic D' (D' = 1). Additionally, association analysis between mutations of the bovine INSIG1 gene and growth traits in Nanyang cattle at 6, 12, 18, and 24 months old was performed, and the results indicated that the polymorphisms were not significantly associated with body mass.
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Jiajie
J
Sun
Yuan
Y
Gao
Dong
D
Liu
Wei
W
Ma
Jing
J
Xue
Chunlei
C
Zhang
Xianyong
X
Lan
Chuzhao
C
Lei
Hong
H
Chen
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002417:Cattle; D002681:China; D018507:Gene Expression Regulation, Developmental; D005787:Gene Frequency; D006239:Haplotypes; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D007438:Introns; D015810:Linkage Disequilibrium; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational
D047908:Intracellular Signaling Peptides and Proteins