Effect of sodium fluoride and magnesium chloride on different hydroxyproline fractions in rat liver.
Sodium fluoride (NaF) is used for prevention of caries in the form of fluoridated drinking water, fluoride tablets etc. In the present study, the effect of NaF-induced alterations in hydroxyproline (Hyp) and collagen was investigated in rat liver. The effect of pretreatment with MgCl2 on NaF-induced changes in liver Hyp and collagen was also studied. The NaF treatment at 5, 10 and 20 mg/kg body wt (reported LD50 of NaF being 24 mg/kg body wt through intraperitoneal route) caused a significant decrease in free Hyp (P < 0.05), when compared to control rats. The rats treated with 20 mg/kg body wt of NaF showed a significant increase in protein-bound Hyp (P < 0.001), as compared to control group, while of NaF treatment at 5 and 10 mg/kg body wt caused no significant change in protein-bound Hyp. All the doses of NaF had no significant effect on peptide-bound and total Hyp and total collagen. Treatment of with MgCl2 alone (30 mg/kg body wt) or with NaF (10 mg/kg body wt) caused a significant decrease in free Hyp (P < 0.05). MgCl2 alone and with NaF caused a significant increase (P < 0.05) in total collagen content. Thus, the present study demonstrated that NaF had no significant effect on total Hyp and collagen, indicating that its use in various products may not interfere with the liver collagen.
Department of Biochemistry, College of Science, PO Box 22452, King Saud University, Riyadh - 11495, Saudi Arabia. nikhat@ksu.edu.sa
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D000818:Animals; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006909:Hydroxyproline / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D015636:Magnesium Chloride / Q000009:adverse effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000009:adverse effects*
D015636:Magnesium Chloride; D012969:Sodium Fluoride; D003094:Collagen; D006909:Hydroxyproline