Comparative fluorescence two-dimensional gel electrophoresis using a gel strip sandwich assembly for the simultaneous on-gel generation of a reference protein spot grid.
The comparison of proteins separated on 2DE is difficult due to gel-to-gel variability. Here, a method named comparative fluorescence gel electrophoresis (CoFGE) is presented, which allows the generation of an artificial protein grid in parallel to the separation of an analytical sample on the same gel. Different fluorescent stains are used to distinguish sample and marker on the gel. The technology combines elements of 1DE and 2DE. Special gel combs with V-shaped wells are placed in a stacking gel above the pI strip. Proteins separated on the pI strip are electrophoresed at the same time as marker proteins (commercially available purified protein of different molecular weight) placed in V-wells. In that way, grids providing approximately 100 nodes as landmarks for the determination of protein spot coordinates are generated. Data analysis is possible with commercial 2DE software capable of warping. The method improves comparability of 2DE protein gels, because they are generated in combination with regular in-gel anchor points formed by protein standards. This was shown here for two comparative experiments with three gels each using Escherichia coli lysate. For a set of 47 well-defined samples spots, the deviation of the coordinates was improved from 7% to less than 1% applying warping using the marker grid. Conclusively, as long as the same protein markers, the same size of pI-strips and the same technology are used, gel matching is reproducibly possible. This is an important advancement for projects involving comparison of 2DE-gels produced over several years and in different laboratories.
Integrated Functional Genomics,, Interdisciplinary Center for Clinical Research, University of Münster, Germany.
Doreen
D
Ackermann
Weiqun
W
Wang
Benjamin
B
Streipert
Birgit
B
Geib
Lothar
L
Grün
Simone
S
König
D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation* / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007526:Isoelectric Point; D011506:Proteins / Q000032:analysis*
D005456:Fluorescent Dyes; D011506:Proteins