Kinetics of bile salt binding to liposomes revealed by carboxyfluorescein release and mathematical modeling.
We propose a mathematical model for the release of carboxyfluorescein from liposomes whose membrane permeability is modified by the binding of different bile salts to the leaflets of the lipid bilayer. We find that the permeability of the liposomal bilayer depends on the difference in the concentrations of bile salt in the inner and outer leaflets and is only minimally influenced by the total concentration of bile salt in the bilayer. Deoxycholate and cholate are found to behave similarly in enhancing permeability for limited times, whereas the novel bile salt, 12-monoketocholate, flips from the outer to inner leaflet slowly, thereby enhancing membrane permeability for a prolonged time.
Department of Mathematical Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA. hinow@uwm.edu
Peter
P
Hinow
Ami
A
Radunskaya
Ian
I
Tucker
Lin
L
Yang
D001647:Bile Acids and Salts / Q000737:chemistry*; D001692:Biological Transport; D002635:Chenodeoxycholic Acid / Q000031:analogs & derivatives; D002793:Cholic Acids / Q000737:chemistry; D003840:Deoxycholic Acid / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry*; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008962:Models, Theoretical; D010539:Permeability; D013997:Time Factors
D001647:Bile Acids and Salts; D002793:Cholic Acids; D005452:Fluoresceins; D008051:Lipid Bilayers; D008081:Liposomes; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; C024098:6-carboxyfluorescein; C025576:3,7-dihydroxy-12-oxocholanoic acid