Fish oil supplementation maintains adequate plasma arachidonate in cats, but similar amounts of vegetable oils lead to dietary arachidonate deficiency from nutrient dilution.
Because fatty acid (FA) metabolism of cats is unique, effects of dietary fish and vegetable oil supplementation on plasma lipids, lipoproteins, lecithin/cholesterol acyl transferase activities, and plasma phospholipid and esterified cholesterol (EC) FAs were investigated. Cats were fed a commercial diet supplemented with 8 g oil/100 g diet for 4 weeks using either high-oleic-acid sunflower oil (diet H), Menhaden fish oil (diet M), or safflower oil (diet S). When supplemented, diet M contained sufficient arachidonate (AA), but diets H and S were deficient. We hypothesized that diet M would modify plasma lipid metabolism, increase FA long-chain n-3 (LCn-3) FA content but not deplete AA levels. Also, diet S would show linoleic acid (LA) accumulation without conversion to AA, and both vegetable oil supplements would dilute dietary AA content when fed to meet cats' energy needs. Plasma samples on weeks 0, 2, and 4 showed no alterations in total cholesterol or nonesterified FA concentrations. Unesterified cholesterol decreased and EC increased in all groups, whereas lecithin/cholesterol acyl transferase activities were unchanged. Diet M showed significant triacylglycerol lowering and decreased pre-β-lipoprotein cholesterol. Plasma phospholipid FA profiles revealed significant enrichment of 18:1n-9 with diet H, LA and 20:2n-6 with diet S, and FA LCn-3FA with diet M. Depletion of AA was observed with diets H and S but not with diet M. Diet M EC FA profiles revealed specificities for LA and 20:5n-3 but not 22:5n-3 or 22:6n-3. Oversupplementation of some commercial diets with vegetable oils causes AA depletion in young cats due to dietary dilution. Findings are consistent with the current recommendations for at least 0.2 g AA/kg diet and that fish oil supplements provide both preformed LCn-3 polyunsaturated FA and AA.
Companion Animal Nutrition Lab, Department of Veterinary Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4474, USA.
Rebecca J
RJ
Angell
Melena K
MK
McClure
Karen E
KE
Bigley
John E
JE
Bauer
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D016718:Arachidonic Acid / Q000097:blood* / Q000172:deficiency; D002415:Cats; D002784:Cholesterol / Q000097:blood; D003677:Deficiency Diseases / Q000097:blood / Q000209:etiology / Q000662:veterinary*; D004041:Dietary Fats / Q000097:blood / Q000494:pharmacology; D019587:Dietary Supplements; D005227:Fatty Acids / Q000097:blood*; D015525:Fatty Acids, Omega-3 / Q000097:blood*; D005395:Fish Oils / Q000097:blood / Q000494:pharmacology*; D019787:Linoleic Acid / Q000097:blood*; D019301:Oleic Acid / Q000097:blood / Q000494:pharmacology; D010743:Phospholipids / Q000097:blood / Q000737:chemistry; D010938:Plant Oils / Q000378:metabolism / Q000494:pharmacology*; D012450:Safflower Oil / Q000378:metabolism / Q000494:pharmacology; D000074242:Sunflower Oil; D014166:Transferases / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
D004041:Dietary Fats; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D010743:Phospholipids; D010938:Plant Oils; D000074242:Sunflower Oil; D014280:Triglycerides; C033266:Menhaden oil; D016718:Arachidonic Acid; D019301:Oleic Acid; D012450:Safflower Oil; D002784:Cholesterol; D019787:Linoleic Acid; D014166:Transferases