Expression of Monstera deliciosa agglutinin gene (mda) in tobacco confers resistance to peach-potato aphids.
The aphid is one of the most serious pests that causes damage to crops worldwide. Lectins from Araceae plant had been proved useful to control the aphid. Herein, the full-length cDNA of Monstera deliciosa agglutinin (mda) gene was cloned and then introduced into tobacco and the influence of the expression of mda in transgenic tobacco against peach-potato aphids (Myzus persicae) was investigated. Among 92 regenerated plants, 59 positive tobacco lines were obtained. Real-time PCR assays and aphid bioassay test revealed that there is a positive correlation between the expression level of mda and the inhibitory effect on peach-potato aphids. The average anti-pests ability of mda transgenic tobacco was 74%, which was higher than that of other reported lectins from Araceae plant. These results indicated that MDA is one of promising insect resistance proteins selected for the control of peach-potato aphids.
Research and Development Center of Plant Resources, Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, People's Republic of China. gykai@shnu.edu.cn
Guoyin
G
Kai
Qian
Q
Ji
Yang
Y
Lu
Zhongying
Z
Qian
Lijie
L
Cui
D000373:Agglutinins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000378:metabolism*; D029064:Araceae / Q000235:genetics* / Q000378:metabolism; D001681:Biological Assay; D001709:Biotechnology / Q000379:methods; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005786:Gene Expression Regulation; D005821:Genetic Techniques; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010802:Phylogeny; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
D000373:Agglutinins; D018076:DNA, Complementary; D018749:RNA, Plant