Anococcygeal raphe revisited: a histological study using mid-term human fetuses and elderly cadavers.
OBJECTIVE
We recently demonstrated the morphology of the anococcygeal ligament. As the anococcygeal ligament and raphe are often confused, the concept of the anococcygeal raphe needs to be re-examined from the perspective of fetal development, as well as in terms of adult morphology.


METHODS
We examined the horizontal sections of 15 fetuses as well as adult histology. From cadavers, we obtained an almost cubic tissue mass containing the dorsal wall of the anorectum, the coccyx and the covering skin. Most sections were stained with hematoxylin and eosin or Masson-trichrome solution.


RESULTS
The adult ligament contained both smooth and striated muscle fibers. A similar band-like structure was seen in fetuses, containing: 1) smooth muscle fibers originating from the longitudinal muscle coat of the anal canal and 2) striated muscle fibers from the external anal sphincter (EAS). However, in fetuses, the levator ani muscle did not attach to either the band or the coccyx. Along and around the anococcygeal ligament, we did not find any aponeurotic tissue with transversely oriented fibers connecting bilateral levator ani slings. Instead, in adults, a fibrous tissue mass was located at a gap between bilateral levator ani slings; this site corresponded to the dorsal side of the ligament and the EAS in the immediately deep side of the natal skin cleft.


CONCLUSIONS
We hypothesize that a classically described raphe corresponds to the specific subcutaneous tissue on the superficial or dorsal side of the anococcygeal ligament.
Division of Colon and Rectal Surgery, Shizuoka Cancer Center Hospital, Shizuoka, Japan. y.kinugasa@scchr.jp
Yusuke
Y
Kinugasa
Takashi
T
Arakawa
Hiroshi
H
Abe
Shinichi
S
Abe
Baik Hwan
BH
Cho
Gen
G
Murakami
Kenichi
K
Sugihara
D000369:Aged, 80 and over; D001003:Anal Canal / Q000033:anatomy & histology* / Q000196:embryology; D002102:Cadaver; D005260:Female; D005333:Fetus; D006801:Humans; D008297:Male; D009130:Muscle, Smooth / Q000033:anatomy & histology* / Q000196:embryology; D012007:Rectum / Q000033:anatomy & histology* / Q000196:embryology