Superparamagnetic iron oxide nanoparticles conjugated to a grass pollen allergen and an optical probe.
In this study we report the development of a bioconjugate between superparamagnetic iron oxide nanoparticles and Phl p5a (one of the major allergens from grass pollen). The bioconjugate also contains an optical probe (Alexa647) conjugated to the nanoparticle via biotin-streptavidin association. We show that this conjugate has a range of features that makes it a very promising candidate to image the localization of this allergen in vivo: (a) upon conjugation to the iron oxide nanoparticles, the allergen retains its ability to interact with IgE antibodies; (b) the magnetic properties of the iron oxide core of this bioconjugate are suitable for MR imaging; and (c) Alexa647 fluorophore retains its emission properties once attached to the iron oxide nanoparticles, yielding a dual modality MRI-optical probe.
Department of Chemistry, Imperial College London, UK.
Fernando
F
Herranz
Carsten B
CB
Schmidt-Weber
Mohamed H
MH
Shamji
Annemarie
A
Narkus
Jesus
J
Ruiz-Cabello
Ramon
R
Vilar
D000485:Allergens / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism; D001710:Biotin / Q000378:metabolism; D002232:Carbocyanines / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D055095:Optics and Photonics; D010940:Plant Proteins / Q000378:metabolism*; D011058:Pollen / Q000378:metabolism*; D019809:Streptavidin / Q000378:metabolism
C569686:Alexa Fluor 647; D000485:Allergens; D002232:Carbocyanines; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C076049:Phl p V protein, Phleum pratense; D010940:Plant Proteins; C000499:ferric oxide; D007073:Immunoglobulin E; D001710:Biotin; D019809:Streptavidin