Decreased serum levels of nucleolin protein fragment, as analyzed by bead-based proteomic technology, in multiple sclerosis patients compared to controls.
In order to investigate the biomarkers associated with relapsing-remitting multiple sclerosis (RRMS), we analyzed 72 patients with RRMS and 65 healthy controls using proteome technology. Peptides in sera were purified using magnetic beads, and analyzed by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and ClinProTool software. Thirteen peptides were significantly different between patients with RRMS and healthy controls. Furthermore, a pattern of peaks was selected for genetic algorithm (GA), supervised neural network (SNN) and quick classifier (QC) model building. Among these three models, GA method was best with 93.49% of recognition capability and 82.66% of cross-validation and discriminated the proteomic spectra in patients with RRMS from healthy controls, with a sensitivity of 80% and a specificity of 91.3%. Meanwhile, the first peptide with m/z 2023.3 was identified as fragment of nucleolin protein. There is a possible relationship between the fragment peptide of nucleolin and the trigger of relapse in MS. Sera nucleolin may serve as a possible biomarker of RRMS.
Department of Neurology, Xuan Wu Hospital of Capital Medical University, Beijing, 100053, PR China.
Jianghong
J
Liu
Linlin
L
Yin
Huiqing
H
Dong
Erhe
E
Xu
Lan
L
Zhang
Yuchen
Y
Qiao
Yuan
Y
Liu
Lin
L
Li
Jianping
J
Jia
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood*; D010750:Phosphoproteins / Q000097:blood*; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D016601:RNA-Binding Proteins / Q000097:blood*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult; D000097796:Nucleolin
D015415:Biomarkers; D010750:Phosphoproteins; D020543:Proteome; D016601:RNA-Binding Proteins; D000097796:Nucleolin