Isolation and characterization of a novel noncoding RNA from nickel-induced lung cancer.
Noncoding RNAs have drawn significant attention in carcinogenesis. In this study, we identified a novel gene named nickel-related gene1 (NRG1) associated with nickel-induced cancer. By using rapid amplification of cDNA end PCR, we obtained the full length of the cDNA. The sequence was analyzed by using related bioinformatics software and comparative genomics methods. The results showed that NRG1 was located on chromosome 2q12, within intron2 of ADAMTS6, a disintegrin and metalloproteinase with thrombospondin motifs. And, NRG1 had a high level of homology (76 %) to rat LINE1 sequence RL1.3 (long interspersed middle repetitive DNA). What's more, there was no continuous open reading frame present in NRG1 sequence. Taken together, these data demonstrate that NRG1 is a novel noncoding RNA, and we predicted it may be a transposon-like gene. The identification of NRG1 emphasized the potential role of noncoding RNA in nickel carcinogenesis.
School of Life Science and Technology, Tongji University, Shanghai, 200092, China.
Jing
J
Zhang
Yang
Y
Zhou
Youjun
Y
Wu
Lin
L
Ma
Yingying
Y
Fan
Xuan
X
Kang
Hongjun
H
Shi
Jun
J
Zhang
D051722:ADAM Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002273:Carcinogens / Q000633:toxicity*; D005260:Female; D007438:Introns; D020084:Long Interspersed Nucleotide Elements; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D019042:Muscle Neoplasms / Q000139:chemically induced / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D009532:Nickel / Q000008:administration & dosage / Q000633:toxicity*; D022661:RNA, Untranslated / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018626:Retroelements / Q000187:drug effects*; D012689:Sequence Homology, Nucleic Acid
D002273:Carcinogens; D022661:RNA, Untranslated; D018626:Retroelements; D009532:Nickel; D051722:ADAM Proteins; C017558:nickel sulfide