Recombinant VirB5 protein as a potential serological marker for the diagnosis of bovine brucellosis.
The molecular tag vaccine against Brucella abortus and serological testing are the main methods of prevention of brucellosis used currently. They can discriminate vaccinated animals and humans from those naturally infected. In this study, we constructed a gene deletion mutant strain, B. abortus S19 virB5 with a molecular tag. Recombinant VirB5 was expressed and purified for evaluation as a diagnostic reagent for bovine brucellosis. In total, 400 sera samples were tested using a VirB5 antigen-based enzyme-linked immunosorbent assay (ELISA) and the results were compared with those of the standard tube agglutination test (SAT). This showed that the sensitivity was 88.2%, specificity was 97.8% and accuracy was 94.8%. Recombinant VirB5 could also be used to discriminate B. abortus-infected mice from mice infected with the B. abortus S19 virB5 mutant strain. It was concluded that recombinant VirB5 could be used as a potential antigen and serological marker for the diagnosis of bovine brucellosis.
College of Animal Science and Veterinary Medicine, Jilin University, No. 5333 Xi’an Road, Changchun 130062, China.
Wei
W
Tan
Xiu-ran
XR
Wang
Ying
Y
Nie
Chong
C
Wang
Li-qing
LQ
Cheng
Xiao-cen
XC
Wang
Rui
R
Zhang
Guang-mou
GM
Yan
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D002003:Brucella abortus / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D011994:Recombinant Proteins