Immunomodulatory influence of bone marrow-derived mesenchymal stem cells on neuroinflammation in astrocyte cultures.
The therapeutic benefits associated with mesenchymal stem cells (MSCs) largely result from their immunomodulatory and neurotrophic properties. In this study, we evaluated the effects of MSCs on astrocyte cultures exposed to lipopolysaccharide. In response to this inflammatory trigger, astrocytes showed an increased expression of pro-inflammatory genes (IL-1β, TNFα, IL-6), which was attenuated by pre-exposure to MSC conditioned medium. Furthermore, mediators released by MSCs increased cell proliferation and altered the regulation of intermediate filaments (GFAP, vimentin), pro-inflammatory enzymes (iNOS, COX-2) and receptors (TLR4, CD14, mGluR3, mGluR5). These data demonstrate that MSCs influence diverse cell types participating in the response to neuroinflammation.
Institute of Neuroscience (IoNS), Group of Neuropharmacology, Université catholique de Louvain, Av. Hippocrate 54, Brussels, Belgium.
Sabrina
S
Schäfer
André-Guilhem
AG
Calas
Maxime
M
Vergouts
Emmanuel
E
Hermans
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology; D007150:Immunohistochemistry; D007155:Immunologic Factors / Q000378:metabolism* / Q000494:pharmacology; D056747:Immunomodulation / Q000187:drug effects / Q000276:immunology*; D007249:Inflammation / Q000139:chemically induced; D008070:Lipopolysaccharides / Q000276:immunology / Q000633:toxicity; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017077:Culture Media, Conditioned; D007155:Immunologic Factors; D008070:Lipopolysaccharides