Evaluation of multidrug resistance in 46 newly diagnosed patients with acute leukemia.
OBJECTIVE
The aim of the present study was to assess the expression profile of multidrug resistance (MDR)-related proteins ABCB1, ABCC1 and LRP in 46 patients with acute leukemia (AL).


METHODS
The levels of MDR gene mRNA expression and protein expression at diagnosis were analyzed by semi-quantitative PCR and flow cytometry, respectively.


RESULTS
In the adult group, higher expression levels of abcc1 gene were associated with older age and lower levels of lactate dehydrogenase (LDH). In the pediatric group, abcc1 gene expression levels were associated with higher CD34 expression and a higher ABCB1 protein expression was correlated with high WBC counts.


CONCLUSIONS
The present data indicate that abcb1 gene overexpression may be associated with a poor prognosis in adults with AL and that ABCB1 and abcc1 expression correlates with different prognostic factors in pediatric patients with AL. Our findings demonstrate that the method of choice to evaluate chemotherapy resistance-related proteins is a major variable.
Departamento de Análises Clínicas, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, Brazil.
Ana Carolina Rabello
AC
de Moraes
Marley Aparecida
MA
Licínio
Júlio Araújo
JA
Zampirolo
Susie Coutinho
SC
Liedke
Joanita Angela Gonzaga
JA
Del Moral
Marcos José
MJ
Machado
Maria Luiza
ML
Bazzo
Maria Cláudia Santos
MC
da Silva
D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics* / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D019008:Drug Resistance, Neoplasm; D015870:Gene Expression; D006801:Humans; D007223:Infant; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D015456:Leukemia, Biphenotypic, Acute / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D007958:Leukocyte Count; D008875:Middle Aged; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D012307:Risk Factors; D016896:Treatment Outcome; D020394:Vault Ribonucleoprotein Particles / Q000235:genetics* / Q000378:metabolism; D000099187:Major Vault Protein
D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018952:Antigens, CD34; D000970:Antineoplastic Agents; D007770:L-Lactate Dehydrogenase; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein; C513055:ABCB1 protein, human; C421109:multidrug resistance-associated protein 1