The protease-activated receptor 1 possesses a functional and cleavable signal peptide which is necessary for receptor expression.
The protease-activated receptor 1 (PAR1) is activated by thrombin cleavage releasing the physiologically-relevant parstatin peptide (residues 1-41). However, the actual length of parstatin was unclear since the receptor may also possess a cleavable signal peptide (residues 1-21) according to prediction programs. Here, we show that this putative signal peptide is indeed functional and removed from the PAR1 resolving the question of parstatin length. Moreover, we show that the sequence encoding the signal peptide may surprisingly play a role in stabilization of the PAR1 mRNA, a function which would be novel for a G protein-coupled receptor.
Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Str. 10, 13125 Berlin, Germany.
Dimitris E
DE
Zampatis
Claudia
C
Rutz
Jens
J
Furkert
Antje
A
Schmidt
Doreen
D
Wüstenhagen
Stefan
S
Kubick
Nikos E
NE
Tsopanoglou
Ralf
R
Schülein
D018076:DNA, Complementary / Q000378:metabolism; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D009690:Nucleic Acid Conformation; D010446:Peptide Fragments / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D044463:Receptor, PAR-1 / Q000737:chemistry / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013917:Thrombin / Q000737:chemistry
D018076:DNA, Complementary; C535229:PAR-1 receptor (1-41), human; D010446:Peptide Fragments; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D044463:Receptor, PAR-1; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; D013917:Thrombin