CuO thin film based uric acid biosensor with enhanced response characteristics.
An efficient reagentless uric acid biosensor has been realized using a copper oxide (CuO) thin film matrix grown onto platinum (Pt) coated corning glass substrates by pulsed laser deposition (PLD) technique. The p-type CuO matrix successfully introduces redox property in the electrode and provides enhanced electron communication features. Sensing response obtained by the bioelectrocatalytic oxidation of uric acid by uricase/CuO/Pt/glass electrode was studied without any external mediator using cyclic voltammetry (CV) and photometric assay. The studies reveal that the uricase/CuO/Pt/glass bio-electrode exhibits good linearity over a wide range of 0.05 mM to 1.0mM uric acid concentration with enhanced response of 2.7 mA/mM and high shelf life (>14 weeks). A low Michaelis-Menten constant (K(m)) of 0.12 mM, indicate that the immobilized enzyme (uricase) has enhanced affinity towards its analyte (uric acid). The results confirm promising application of the p-type CuO thin film matrix for the realization of a reagentless integrated implantable biosensor.
Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India.
Kajal
K
Jindal
Monika
M
Tomar
Vinay
V
Gupta
D015374:Biosensing Techniques / Q000295:instrumentation*; D003300:Copper / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry; D012680:Sensitivity and Specificity; D014503:Urate Oxidase / Q000378:metabolism; D014527:Uric Acid / Q000032:analysis / Q000097:blood* / Q000378:metabolism
D004800:Enzymes, Immobilized; D014527:Uric Acid; D010984:Platinum; D003300:Copper; D014503:Urate Oxidase; C000520:cuprous oxide