[Bacteriological diagnosis of tuberculosis].
Today, microscopic examination and culture are still essential for active tuberculosis diagnosis and therapeutic decisions. Nucleic acid amplification and line probe assays speed up identification and susceptibility testing of mycobacteria in AFB smear positive specimens or in culture. They are also efficient for comparison of M. tuberculosis strains with each other (genotyping). On the other hand, today, molecular tests are not relevant for the diagnosis in smear negative specimens and even more for diagnosis of culture-negative tuberculosis.
UPMC université Paris-6, ER5, EA 1541, Laboratoire de bactériologie-hygiéne, F-75005 Paris. nicolas.veziris@upmc.fr
Nicolas
N
Veziris
D001431:Bacteriological Techniques / Q000379:methods*; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques; D012680:Sensitivity and Specificity; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology*