Ischemia-reperfusion injury of the mouse kidney.
Studies of the complex responses of the kidney to acute injury have yielded important insights into mechanisms of tissue injury and repair. A variety of injury models have contributed to this impressive body of knowledge, but the ischemia-reperfusion (IR) model has perhaps been the most widely used. This chapter contains a detailed method description for IR injury in the mouse together with notes on blood sampling and tissue harvesting. The aim of the chapter is to provide the novice with a step-by-step guide to establishing this procedure in their research program.
Maine Medical Center Research Institute, Scarborough, ME, USA. Oxburl@mmc.org
Leif
L
Oxburgh
Mark P
MP
de Caestecker
D018681:Anesthetics, General / Q000627:therapeutic use; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006993:Hypnotics and Sedatives / Q000627:therapeutic use; D007530:Isoflurane / Q000627:therapeutic use; D007649:Ketamine / Q000627:therapeutic use; D007668:Kidney / Q000473:pathology*; D008297:Male; D051379:Mice; D009399:Nephrons / Q000473:pathology; D015427:Reperfusion Injury / Q000097:blood / Q000473:pathology*; D014991:Xylazine / Q000627:therapeutic use
D018681:Anesthetics, General; D006993:Hypnotics and Sedatives; D014991:Xylazine; D007649:Ketamine; D007530:Isoflurane