Changes of myogenic reactive oxygen species and interleukin-6 in contracting skeletal muscle cells.
The aim of this study was to measure changes in myotube reactive oxygen species (ROS) and the production of interleukin (IL)-6 in electrically stimulated mouse C2C12 skeletal muscle cells. After five days of differentiation, myotubes were stimulated using an electrical stimulator set at 45 V at a frequency of 5 Hz, with a pulse width of 20 ms. Acute stimulations were performed for 45, 60, 75, 90, or 120 min in each dish. ROSs were detected in the extracted cells directly using a fluorescent probe. IL-6 mRNA expression in C2C12 myotubes and IL-6 concentration in C2C12 myotube supernatants were determined using real-time PCR and ELISA, respectively. Compared with control cells, ROS generation was significantly increased at 45 min after the onset of stimulation (P < 0.01) and continued to increase, reaching a maximum at 120 min. IL-6 mRNA expression and IL-6 concentration in C2C12 cells were significantly increased after 75 min (P < 0.01) and 120 min (P < 0.05) of electrical stimulation (ES) compared with the control cells. Our data show that a specific ES intensity may modulate ROS accumulation and affect IL-6 gene expression in contracting skeletal muscle cells.
College of Sports Science, South China Normal University, Guangzhou 510006, China.
Hongying
H
Pan
Xiaoyang
X
Xu
Xuanming
X
Hao
Yajun
Y
Chen
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002459:Cell Fusion; D002460:Cell Line; D004558:Electric Stimulation; D005786:Gene Expression Regulation; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D032342:Muscle Cells / Q000166:cytology / Q000378:metabolism*; D009119:Muscle Contraction / Q000502:physiology*; D024510:Muscle Development / Q000235:genetics; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013997:Time Factors
D015850:Interleukin-6; D012333:RNA, Messenger; D017382:Reactive Oxygen Species