A novel anti-inflammatory role for spleen-derived interleukin-10 in obesity-induced inflammation in white adipose tissue and liver.
Obesity is associated with systemic low-grade inflammation and obesity-related metabolic disorders. Considering that obesity decreases the expression of proinflammatory cytokines in the spleen, we assessed the role of interleukin (IL)-10, an anti-inflammatory cytokine produced by the spleen, in the pathogenesis of obesity. Changes in obesity-related pathogenesis, including inflammatory responses in multiple organs, were assessed after systemic administration of exogenous IL-10 to splenectomy (SPX)-treated obese wild-type and IL-10 knockout (IL-10KO) mice. Obesity resulted in the inability of the spleen to synthesize cytokines, including IL-10, and proinflammatory cytokines in obesity are then likely to emerge from tissues other than the spleen because serum levels of IL-10, but not proinflammatory cytokines, decreased despite the expression of these cytokines in the spleen being reduced in high fat-induced obese mice. SPX aggravated the inflammatory response in white adipose tissue (WAT) and the liver and suppressed adiposity in WAT. However, it accentuated adiposity in the liver. These SPX-induced changes were inhibited by systemic administration of IL-10. Moreover, SPX had little effect on the inflammatory responses in WAT and the liver of IL-10KO mice. These data show the role of spleen-derived IL-10 in diet-induced changes as a result of inflammatory responses in WAT and the liver.
Department of Internal Medicine 1, Faculty of Medicine, Oita University, Hasama, Yufu, Japan. gotokoro@oita-u.ac.jp
Koro
K
Gotoh
Megumi
M
Inoue
Takayuki
T
Masaki
Seiichi
S
Chiba
Takanobu
T
Shimasaki
Hisae
H
Ando
Kansuke
K
Fujiwara
Isao
I
Katsuragi
Tetsuya
T
Kakuma
Masataka
M
Seike
Toshiie
T
Sakata
Hironobu
H
Yoshimatsu
D052436:Adipose Tissue, White / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D059305:Diet, High-Fat; D004435:Eating; D006505:Hepatitis / Q000209:etiology; D007249:Inflammation / Q000209:etiology* / Q000378:metabolism; D016753:Interleukin-10 / Q000097:blood / Q000502:physiology*; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity / Q000150:complications*; D013154:Spleen / Q000378:metabolism*; D013156:Splenectomy
D016753:Interleukin-10