[Micromorphological observation of bacterial biofilms on ciliated epithelia of chronic rhinosinusitis].
To observe the micromorphological characteristic of bacterial biofilm on mucosa of chronic rhinosinusitis (CRS).



Mucosa samples of middle turbinate were obtained from 4 patients of CRS during ESS. The size of each sample was about 4 mm x 4 mm. The samples were fixed in 4% paraformaldehyde for 24 hours, then fixed in 1% osmium tetroxide for 2 hours, graded dehydration with ethanol, dried with carbon dioxide and sputter coated with gold. The ultrastructure of these samples was observed by scanning electron microscope.



Bacterial biofilms were found on samples in all 4 patients. The biofilms were mainly formed on the surface of cilia. The bacterial flagella and cilia were intertwined. The biofilms could be found in a lot kinds of bacterial infections or mixed infections which were caused by multiple bacteria and fungi.



Bacterial biofilm could be formed on ciliated epithelia.
Department of Otorhinolaryngology, Zhongshan Hospital, Xiamen University, Xiamen, 361004, China. iamyhb@hotmail.com
Haibin
HB
Yang
Yongqing
YQ
Guo
Mingqiang
MQ
Yu
D001424:Bacterial Infections / Q000382:microbiology*; D018441:Biofilms; D002923:Cilia / Q000382:microbiology* / Q000648:ultrastructure; D004848:Epithelium / Q000382:microbiology / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012852:Sinusitis / Q000382:microbiology*