Isolation and culture of rat embryonic neural cells: a quick protocol.
We are describing a quick method to dissociate and culture hippocampal or cortical neurons from E15-17 rat embryos. The procedure can be applied successfully to the isolation of mouse and human primary neurons and neural progenitors. Dissociated neurons are maintained in serum-free medium up to several weeks. These cultures can be used for nucleofection, immunocytochemistry, nucleic acids preparation, as well as electrophysiology. Older neuronal cultures can also be transfected with a good efficiency rate by lentiviral transduction and, less efficiently, with calcium phosphate or lipid-based methods such as lipofectamine.
LSU Health Sciences Center - New Orleans, USA.
Marco
M
Pacifici
Francesca
F
Peruzzi
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology; D004622:Embryo, Mammalian / Q000166:cytology; D005909:Glioblastoma / Q000473:pathology; D006624:Hippocampus / Q000166:cytology* / Q000196:embryology; D006801:Humans; D009474:Neurons / Q000166:cytology*; D051381:Rats