Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles.
Thiol group functionalized silica-coated magnetic nanoparticles (Si-MNPs@SH) were synthesized for rapid and selective magnetic field-based separation of mixed proteins. The highest adsorption efficiencies of binary proteins, bovine serum albumin (BSA; 66 kDa; pI = 4.65) and lysozyme (LYZ; 14.3 kDa; pI = 11) were shown at the pH values corresponding to their own pI in the single-component protein. In the mixed protein, however, the adsorption performance of BSA and LYZ by Si-MNPs@SH was governed not only by pH but also by the molecular weight of each protein in the mixed protein.
Bio-IT Convergence Center, Korea Institute of Ceramic Engineering and Technology, Seoul, 153-801, South Korea. jhchang@kicet.re.kr.
Soo Youn
SY
Lee
Chi Young
CY
Ahn
Jiho
J
Lee
Jin Hyung
JH
Lee
Jeong Ho
JH
Chang