High throughput detection of tetracycline residues in milk using graphene or graphene oxide as MALDI-TOF MS matrix.
In this work, a new pre-analysis method for tetracyclines (TCs) detection from the milk samples was established. As a good accomplishment for the existing accurate quantification strategies for TCs detection, the new pre-analysis method was demonstrated to be simple, sensitive, fast, cost effective, and high throughput, which would do a great favor to the routine quality pre-analysis of TCs from milk samples. Graphene or graphene oxide was utilized, for the first time, as a duel-platform to enrich and detect the TCs by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). All together, four TCs were chosen as models: tetracycline, oxytetracycline, demeclocycline, and chlortetracycline. Due to the excellent electronic, thermal, and mechanical properties, graphene and graphene oxide were successfully applied as matrices for MALDI-TOF MS with free background inference in low mass range. Meanwhile, graphene or graphene oxide has a large surface area and strong interaction force with the analytes. By taking the advantage of these features, TCs were effectively enriched with the limit of detection (LOD) as low as 2 nM.
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
Junyan
J
Liu
Yang
Y
Liu
Mingxia
M
Gao
Xiangmin
X
Zhang
D000818:Animals; D004350:Drug Residues / Q000032:analysis* / Q000302:isolation & purification; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods*; D013754:Tetracyclines / Q000032:analysis* / Q000302:isolation & purification
D010087:Oxides; D013754:Tetracyclines; D006108:Graphite