Genotyping of single nucleotide polymorphisms by 5' nuclease allelic discrimination.
Real-time quantitative PCR is an efficient method for high-throughput genotyping of single nucleotide polymorphisms (SNPs). In this chapter, we describe the 5' nuclease allelic discrimination assay for genotyping biallelic SNPs.
Fred Hutchinson Cancer Research Centre, Seattle, WA, USA.
Mari
M
Malkki
Effie W
EW
Petersdorf
D015720:5'-Nucleotidase / Q000378:metabolism*; D000483:Alleles; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
D015720:5'-Nucleotidase