Natronoarchaeum philippinense sp. nov., a haloarchaeon isolated from commercial solar salt.
A Gram-staining-negative, pleomorphic, aerobic, halophilic archaeon, designated strain 294-194-5(T), was isolated in Japan from commercial solar salt imported from the Philippines. Colonies of strain 294-194-5(T) were translucent and red. Strain 294-194-5(T) was able to grow at 20-50 °C (optimum, 37-45 °C), with 14-30 % (w/v) NaCl (optimum, 18 %), and at pH 6.5-8.5 (optimum, pH 8.0). MgCl2 was not required for growth. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain 294-194-5(T) was most closely related to Natronoarchaeum mannanilyticum YSM-123(T) (96.8-97.1 % sequence similarities). The major polar lipids of the novel strain were the C20C20 and C20C25 derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester and the same glycolipids (disulfated diglycosyl diether and one unidentified glycolipid) as detected in N. mannanilyticum YSM-123(T). The DNA G+C content of strain 294-194-5(T) was 63.0 mol%. The DNA-DNA relatedness values between the novel strain and N. mannanilyticum YSM-123(T)were 46.5 % and 48.5 % (reciprocal). Based on these data, strain 294-194-5(T) represents a novel species of the genus Natronoarchaeum, for which the name Natronoarchaeum philippinense sp. nov. is proposed. The type strain is 294-194-5(T) ( = JCM 16593(T) = CECT 7630(T)).
Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
Yasuhiro
Y
Shimane
Department of Biological Applied Chemistry, Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Shuhei
S
Nagaoka
Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Hiroaki
H
Minegishi
Halophiles Research Institute, 677-1 Shimizu, Noda-shi, Chiba 278-0043, Japan.
Masahiro
M
Kamekura
Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Akinobu
A
Echigo
Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
Yuji
Y
Hatada
Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
Takashi
T
Ito
Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.|Department of Biological Applied Chemistry, Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Ron
R
Usami
D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010679:Philippines; D010715:Phosphatidylglycerols / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
D019641:DNA, Archaeal; D005227:Fatty Acids; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride