Identification of the novel HLA allele, HLA-B*40:159, in a Taiwanese hematopoietic stem cell donor and the probable HLA haplotype in an association with B*40:159.
Using a sequence-based typing method, we found a new HLA-B*40 variant, B*40:159, in a Taiwanese hematopoietic stem cell donor. The sequence of B*40:159 is identical to the sequence of B*40:06:01:01 in exons 2 and 3 except the nucleotides at positions 412 (A → G) and 429 (A → C). The sequence variation caused two amino acid exchanges at residue 114 (N → D) and residue 116 (Y → S). The probable HLA-A, HLA-B, HLA-C and HLA-DRB1 haplotype in association with B*40:159 may be deduced as HLA-A*02:06-B*40:159-C*08:01-DRB1*08:03. The generation of B*40:159 is thought as the result of a sequence recombination event where B*46:01:01:01, B*15:01:01 or B*15:02:01 donated a minimum length of the DNA sequence from residue 412 to residue 419 to the recipient sequence of B*40:06:01:01.
Laboratory of Immunogenetics and Cord Blood Bank, Buddhist Tzu Chi Stem Cells Centre and Marrow Donor Registry, Buddhist Tzu Chi General Hospital, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan. edward@tzuchi.com.tw
K L
KL
Yang
S K
SK
Lee
P Y
PY
Lin
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D056726:Genetic Association Studies; D059947:HLA-B40 Antigen / Q000737:chemistry / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D013624:Taiwan; D014019:Tissue Donors
D059947:HLA-B40 Antigen