Down-regulation of gelsolin may play a role in the progression of inverted papilloma through an antiapoptotic mechanism.
BACKGROUND
Gelsolin is an actin-binding protein with multiple cellular functions including apoptosis and is reported to be down-regulated in various cancers and premalignant lesions. The objective of this study was to identify gelsolin and caspase-3 expression in inverted papilloma (IP) and investigate the role of gelsolin in the progression of IP related to apoptosis.


METHODS
Specimens from 30 patients with nondysplastic IP were retrieved. The percentage of surface epithelium covered with squamous metaplastic epithelium was assessed. Immunohistochemically demonstrated gelsolin and caspase-3 expression were compared between IP and adjacent control mucosa. We analyzed the correlations among gelsolin expression, caspase-3 expression, and the degree of squamous metaplasia in IP.


RESULTS
The degree of squamous metaplasia of surface epithelium was inversely correlated with gelsolin (r = -0.610; p < 0.001) and caspase-3 expression (r = -0.433; p = 0.017). Gelsolin expression in IP was significantly lower than that in the control when >50% of surface epithelium showed squamous metaplasia (p = 0.015). Caspase-3 also showed diminished expression when >50% of surface epithelium had undergone squamous metaplasia (p = 0.035). Gelsolin and caspase-3 expression showed no significant differences when the degree of squamous metaplasia was ≤50%. Gelsolin and caspase-3 expression levels in IP had a positive relationship (r = 0.557; p = 0.001).


CONCLUSIONS
Progression of IP may be related to an insidious decrease in caspase-3-mediated apoptosis, and down-regulated gelsolin expression may be correlated with the decrease in apoptosis, especially in more highly progressed IP in which >50% of surface epithelium has undergone squamous metaplasia.
Department of Otolaryngology, National Police Hospital, Seoul, Korea.
Ju Eun
JE
Cho
Won il
Wi
Park
Dong-chul
DC
Kim
Hee-Jin
HJ
Kim
Soo Whan
SW
Kim
Jun Myung
JM
Kang
Jin Hee
JH
Cho
D000368:Aged; D017209:Apoptosis / Q000235:genetics; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D018260:Gelsolin / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008679:Metaplasia / Q000150:complications / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D009666:Nose / Q000473:pathology*; D009669:Nose Neoplasms / Q000150:complications / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018308:Papilloma, Inverted / Q000150:complications / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D018260:Gelsolin; D053148:Caspase 3