Increased NTPDase activity in lymphocytes during experimental sepsis.
We investigated in rats induced to sepsis the activity of ectonucleoside triphosphate diphosphohydrolase (NTPDase; CD39; E.C. 3.6.1.5), an enzyme involved in the modulation of immune responses. After 12 hours of surgery, lymphocytes were isolated from blood and NTPDase activity was determined. It was also performed the histology of kidney, liver, and lung. The results demonstrated an increase in the hydrolysis of adenosine-5'-triphosphate (ATP) (P < 0.01), but no changes regarding adenosine-5'-monophosphate (ADP) hydrolysis (P > 0.05). Histological analysis showed several morphological changes in the septic group, such as vascular congestion, necrosis, and infiltration of mononuclear cells. It is known that the intracellular milieu contains much more ATP nucleotides than the extracellular. In this context, the increased ATPasic activity was probably induced as a dynamic response to clean up the elevated ATP levels resulting from cellular death.
Centro de Ciências da Saúde, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
Claudia de Mello
Cde M
Bertoncheli
Carine Eloise Prestes
CE
Zimmermann
Jeandre Augusto dos Santos
JA
Jaques
Cláudio Alberto Martins
CA
Leal
Jader Betsch
JB
Ruchel
Bruna Cipolatto
BC
Rocha
Kelly de Vargas
Kde V
Pinheiro
Viviane do Carmo Gonçalves
Vdo C
Souza
Daniel Roulim
DR
Stainki
Sônia Cristina Almeida
SC
Luz
Maria Rosa Chitolina
MR
Schetinger
Daniela Bitencourt Rosa
DB
Leal
D000244:Adenosine Diphosphate / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry*; D001081:Apyrase / Q000737:chemistry*; D016923:Cell Death; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D006868:Hydrolysis; D007107:Immune System; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D009711:Nucleotides / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000201:enzymology* / Q000473:pathology; D014018:Tissue Distribution
D015703:Antigens, CD; D009711:Nucleotides; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D001081:Apyrase; C067869:CD39 antigen