Separation and cryopreservation of lymphocytes from spleen and lymph node.
Spleen and lymph node retrieved post-mortem from deceased organ donors are a rich source of lymphocytes. Storage of lymphocytes separated from these sources can be valuable where post-transplant testing (crossmatching) is required. DNA extraction from stored lymphocytes also allows further genetic testing where required, for example additional HLA typing not performed at the time of transplant for donor-specific antibody monitoring. Methods for the isolation and freezing of such cells is described.
Department of Clinical Immunology, PathWest Laboratory Medicine, Royal Perth Hospital, Perth, WA, Australia.
Gabriella
G
Tassone
Samantha J
SJ
Fidler
D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D006650:Histocompatibility Testing; D006801:Humans; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology; D013154:Spleen / Q000166:cytology* / Q000276:immunology