Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector.
A unique hyperbranched polymeric system with a linear poly-2-dimethylaminoethyl methacrylate (pDMAEMA) block and a hyperbranched polyethylene glycol methyl ether methacrylate (PEGMEMA) and ethylene dimethacrylate (EGDMA) block was designed and synthesized via deactivation enhanced atom transfer radical polymerisation (DE-ATRP) for efficient gene delivery. Using this unique structure, with a linear pDMAEMA block, which efficiently binds to plasmid DNA (pDNA) and hyperbranched polyethylene glycol (PEG) based block as a protective shell, we were able to maintain high transfection levels without sacrificing cellular viability even at high doses. The transfection capability and cytotoxicity of the polymers over a range of pDNA concentration were analysed and the results were compared to commercially available transfection vectors such as polyethylene imine (branched PEI, 25 kDa), partially degraded poly(amido amine)dendrimer (dPAMAM; commercial name: SuperFect(®)) in fibroblasts and adipose tissue derived stem cells (ADSCs).
Network of Excellence for Functional Biomaterials, NFB Building, IDA Business Park, Newcastle Road, Dangan, National University of Ireland, Galway, Ireland. a.mathew2@nuigalway.ie
Asha
A
Mathew
Hongliang
H
Cao
Estelle
E
Collin
Wenxin
W
Wang
Abhay
A
Pandit
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002470:Cell Survival; D004247:DNA / Q000008:administration & dosage*; D050091:Dendrimers / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D009757:Nylons / Q000737:chemistry; D010957:Plasmids; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D011817:Rabbits; D013234:Stem Cells / Q000378:metabolism; D014162:Transfection
D050091:Dendrimers; D008689:Methacrylates; D009757:Nylons; C104700:PAMAM Starburst; C407037:poly(2-(dimethylamino)ethyl methacrylate); C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; C004919:ethylene dimethacrylate; D011094:Polyethyleneimine; D004247:DNA