Internal ribosomal entry site-mediated translation is important for rhythmic PERIOD1 expression.
The mouse PERIOD1 (mPER1) plays an important role in the maintenance of circadian rhythm. Translation of mPer1 is directed by both a cap-dependent process and cap-independent translation mediated by an internal ribosomal entry site (IRES) in the 5' untranslated region (UTR). Here, we compared mPer1 IRES activity with other cellular IRESs. We also found critical region in mPer1 5'UTR for heterogeneous nuclear ribonucleoprotein Q (HNRNPQ) binding. Deletion of HNRNPQ binding region markedly decreased IRES activity and disrupted rhythmicity. A mathematical model also suggests that rhythmic IRES-dependent translation is a key process in mPER1 oscillation. The IRES-mediated translation of mPer1 will help define the post-transcriptional regulation of the core clock genes.
Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea.
Kyung-Ha
KH
Lee
Sung-Hoon
SH
Kim
Do-Yeon
DY
Kim
Seunghwan
S
Kim
Kyong-Tai
KT
Kim
D020121:5' Untranslated Regions; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002940:Circadian Rhythm / Q000235:genetics*; D005786:Gene Expression Regulation; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism; D051379:Mice; D008962:Models, Theoretical; D056950:Period Circadian Proteins / Q000235:genetics*; D010766:Phosphorylation; D014176:Protein Biosynthesis
D020121:5' Untranslated Regions; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C486686:Hnrpr protein, mouse; C539023:Per1 protein, mouse; D056950:Period Circadian Proteins