Anticoagulant activity of Moon jellyfish (Aurelia aurita) tentacle extract.
Moon jellyfish (Aurelia aurita) tentacle extract was studied for its anticoagulant activity in vitro. The Jellyfish Tentacle Extract (JFTE) showed very strong fibrinogenolytic activity by cleaving Aα and Bβ chain of fibrinogen molecule. The fibrinogenolytic activity was found to be stronger than some snake venom derived anticoagulants. JFTE also completely liquefied fibrin clots in 24 h. JFTE was found to contain both high and low molecular weight proteins/peptides. The fibrinogenolysis appears to be caused by high molecular weight fractions of the extract. It has been also noted that PMSF significantly reduced fibrinogenolytic activity and heating totally abolished it. Autolytic degradation of the high molecular weight protein was also noted. Autolysis slowed down, but did not abolish the fibrinogenolytic activity of the extract.
Department of Biological Sciences, Birla Institute of Technology and Science-Pilani, K.K. Birla Goa Campus, Zuarinagar, Goa 403726, India.
Akriti
A
Rastogi
Sumit
S
Biswas
Angshuman
A
Sarkar
Dibakar
D
Chakrabarty
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry*; D017097:Electric Impedance; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000737:chemistry*; D005343:Fibrinolytic Agents / Q000737:chemistry*; D053878:Hemolytic Agents / Q000737:chemistry*; D006801:Humans; D007194:India; D008970:Molecular Weight; D010974:Platelet Aggregation / Q000187:drug effects; D037401:Scyphozoa / Q000737:chemistry*; D014020:Tissue Extracts / Q000737:chemistry*
D000925:Anticoagulants; D005343:Fibrinolytic Agents; D053878:Hemolytic Agents; D014020:Tissue Extracts; D005340:Fibrinogen