Effects of aging and anatomic location on gene expression in human retina.
OBJECTIVE
To determine the effects of age and topographic location on gene expression in human neural retina.


METHODS
Macular and peripheral neural retina RNA was isolated from human donor eyes for DNA microarray and quantitative RT-PCR analyses.


RESULTS
Total RNA integrity from human donors was preserved. Hierarchical clustering analysis demonstrates that the gene expression profiles of young, old, macula, and peripheral retina cluster into four distinct groups. Genes which are highly expressed in macular, peripheral, young, or old retina were identified, including inhibitors of Wnt Signaling Pathway (DKK1, FZD10, and SFRP2) which are preferably expressed in the periphery.


CONCLUSIONS
The transcriptome of the human retina is affected by age and topographic location. Wnt pathway inhibitors in the periphery may maintain peripheral retinal cells in an undifferentiated state. Understanding the effects of age and topographic location on gene expression may lead to the development of new therapeutic interventions for age-related eye diseases.
Department of Ophthalmology, Harkness Eye Institute, Columbia University, New York NY, USA.
Hui
H
Cai
Mark A
MA
Fields
Risa
R
Hoshino
Lucian V Del
LV
Priore