Differential collagenolytic activity of Candida albicans isolated from oral mucosa and dentinal carious lesions of HIV-infected children.
OBJECTIVE
The aim of this study was to compare type I collagen degradation by Candida albicans isolated from oral mucosa (M) and cavitated active dentinal caries (CAD) of HIV-infected children.


METHODS
To verify the proteolytic activity, the specimens were cultivated in brain-heart infusion medium and the supernatants were incubated in the presence or absence of type I collagen at 37°C for 12 hours and analyzed using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intensity of the bands on the gels was assessed by densitometric analysis using a scanner and images analyzed with software from Kodak Digital Science EDAS 120.


RESULTS
Supernatants of all the C. albicans degraded type I collagen: that from M, on average, by 38.3% (SD 21.67) and that from CAD by 54% (SD 25.94; Wilcoxon test: P < .05). Predisposing factors had no association with the percentage of type I collagen degradation (Mann-Whitney test: P > .05).


CONCLUSIONS
Candida albicans from different sites of the oral cavity of HIV-infected children has proteolytic activity for type I collagen.
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Maristela Barbosa
MB
Portela
Madeleine Souza
MS
das Chagas
Daniella Ferreira
DF
Cerqueira
Ivete Pomarico Ribeiro
IP
de Souza
Thaís
T
Souto-Padrón
Rosangela Maria
RM
de Araújo Soares
Gloria Fernanda Barbosa
GF
de Araújo Castro
D002176:Candida albicans / Q000201:enzymology*; D002648:Child; D002675:Child, Preschool; D024042:Collagen Type I / Q000378:metabolism*; D003731:Dental Caries / Q000150:complications / Q000382:microbiology*; D003804:Dentin / Q000382:microbiology; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000382:microbiology*; D059748:Proteolysis; D018709:Statistics, Nonparametric
D024042:Collagen Type I