An analysis of the concentration change of intermediate metabolites by gene manipulation in fatty acid biosynthesis.
In this report, concentration of malonic acid and acetic acid produced in Escherichia coli were investigated by the expression of acetyl-CoA carboxylase genes (accs) and a malonyl-CoA:ACP transacylase gene (fabD). Both malonyl-CoA and acetyl-CoA are essential intermediate metabolites in the fatty acid biosynthetic pathway, and are reversibly transformed to malonic acid and acetic acid, respectively in the cell. Acetyl-CoA is converted to malonic-CoA by acetyl-CoA carboxylases (Accs), which are composed of 3 different subunits (AccA, AccB, and AccC), and the resulting malonyl-CoA is then converted to malonyl-[acp] by malonyl-CoA:ACP transacylase (FabD). In this study, these genes were separately cloned, and the influences of overexpression of 4 different genes on the concentration of malonic acid and acetic acid were analyzed. Compared with the wild type E. coli, a recombinant strain containing 3 acc genes together showed a 41.03% enhanced malonic acid production, and a 4.29-fold increased ratio of malonic acid to acetic acid.
Department of Chemical Engineering, Hongik University, Seoul 121-791, Republic of Korea.
Seo-Young
SY
Park
Eunyoung
E
Jeon
Sunhee
S
Lee
Jinwon
J
Lee
Taiyong
T
Lee
Jong-In
JI
Won
D019342:Acetic Acid / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D051080:Acyl-Carrier Protein S-Malonyltransferase / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D054889:Fatty Acid Synthase, Type II / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D005798:Genes, Bacterial; D008314:Malonates / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D005227:Fatty Acids; D008314:Malonates; C030290:malonic acid; D051080:Acyl-Carrier Protein S-Malonyltransferase; C494968:fabD protein, E coli; D054889:Fatty Acid Synthase, Type II; D000103:Acetyl-CoA Carboxylase; C557124:accA protein, E coli; C557125:accC protein, E coli; C079633:biotin carboxyl carrier protein; D019342:Acetic Acid