Intra-amygdala infusion of the protein kinase Mzeta inhibitor ZIP disrupts foreground context fear memory.
Protein kinase Mzeta has been the subject of much recent interest, as it is the only molecule currently identified to maintain memory. Despite the wealth of studies investigating PKMζ in memory, questions remain about which types of memory PKMζ supports. Further, it is unclear how long the inhibitor of PKMz, ζ-pseudosubstrate inhibitory peptide (ZIP) remains in the brain after infusion. Here, we demonstrate that foreground context fear memory requires PKMζ activity in the amygdala. We also show that ZIP is fully cleared from the brain by 24h after infusion. These data contribute to a growing body of literature that demonstrates that PKMζ plays a key role in maintaining amygdala-dependent memory and provides new information about the degradation timecourse of the most commonly used inhibitor of PKMζ, ZIP.
Department of Psychology, University of Wisconsin-Milwaukee, 2441 Hartford Ave., Milwaukee, WI 53211, USA.
Janine L
JL
Kwapis
Timothy J
TJ
Jarome
Marieke R
MR
Gilmartin
Fred J
FJ
Helmstetter
D000679:Amygdala / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001245:Association Learning / Q000187:drug effects / Q000502:physiology*; D057846:Cell-Penetrating Peptides; D005239:Fear; D055666:Lipopeptides / Q000494:pharmacology; D008297:Male; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D051381:Rats; D020318:Rats, Long-Evans; D012153:Retention, Psychology / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
D057846:Cell-Penetrating Peptides; D055666:Lipopeptides; C568285:zeta-inhibitory peptide; D011493:Protein Kinase C; C504730:protein kinase M zeta, rat