Functionalization of polycarbonate with proteins; open-tubular enzymatic microreactors.
This paper examines a set of techniques for the immobilization of enzymes on the surface of microchannels fabricated in polycarbonate (PC). Our experiments identify the method that uses combined physico-chemical immobilization on a layer of polyethyleneimine (PEI) as a reproducible vista for the robust immobilization of proteins. As an example, we demonstrate the fabrication, throughput and stability of an open-tubular reactor draped with alkaline phosphatase (ALP, EC 3.1.3.1) as a model enzyme. As PC is suitable for industrial applications the method could potentially be used to immobilize proteins in numbered-up implementations.
Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland. ogonczyk@ichf.edu.pl
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Ogończyk
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Jankowski
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Garstecki
D000469:Alkaline Phosphatase / Q000737:chemistry*; D019149:Bioreactors; D002234:Carbodiimides / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D011076:Polycarboxylate Cement / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D055672:Static Electricity; D014510:Urease / Q000737:chemistry
D002234:Carbodiimides; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D011076:Polycarboxylate Cement; C005506:polycarbonate; D011094:Polyethyleneimine; D000469:Alkaline Phosphatase; D014510:Urease