H. pylori clinical isolates have diverse babAB genotype distributions over different topographic sites of stomach with correlation to clinical disease outcomes.
BACKGROUND
Intragenomic recombination between babA and babB mediates antigenic variations and may help H. pylori colonization. This study determined whether variable genotypes of babA and babB correlate to different clinical disease outcomes, and can distribute over the different gastric niches.


RESULTS
This study enrolled 92 clinical strains (45 from peptic ulcer, 27 from gastritis, and 20 from gastric cancer) to detect whether the babA and babB are at locus A or B by PCR reactions using the primers designed from the upstream and variable region of the babA and babB genes. Four genotypes of babA and babB (A B, AB B, A AB, AB AB) were found. The distribution of the 4 genotypes in 92 clinical strains was significantly different among patients with different gastric diseases (p < 0.05). The isolates from gastric cancer patients had a higher rate of AB AB genotype than those from non-cancer patients (40.0% vs. 9.7%, p < 0.05). The AB AB genotype was associated with a higher intensity of intestinal metaplasia (p < 0.05), but did not correlate with a higher inflammation and colonization density in gastric histology (p > 0.05). Besides, the study enrolled 19 patients to verify whether variable genotypes of babAB existed in the different gastric niches. Among the patients infected with more than one babAB genotypes over antrum and corpus, there were higher rate of genotypes as A B or AB AB in isolates from antrum than in those from corpus (75.0 % vs. 16.7%, p < 0.05).


CONCLUSIONS
The H. pylori isolate with the AB AB genotype correlates with an increased gastric cancer risk, and colonize in an antrum predominant manner.
Department of Internal Medicine, College of Medicine, National Cheng Kung University, 138 Sheng Li Road, Tainan, Taiwan.
Shew-Meei
SM
Sheu
Bor-Shyang
BS
Sheu
Wen-Cheng
WC
Chiang
Cheng-Yen
CY
Kao
Hsiu-Mei
HM
Wu
Hsiao-Bai
HB
Yang
Jiunn-Jong
JJ
Wu
D018829:Adhesins, Bacterial / Q000235:genetics*; D019943:Amino Acid Substitution; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005756:Gastritis / Q000382:microbiology / Q000473:pathology; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D010437:Peptic Ulcer / Q000382:microbiology / Q000473:pathology; D017422:Sequence Analysis, DNA; D013270:Stomach / Q000033:anatomy & histology / Q000382:microbiology* / Q000473:pathology; D013274:Stomach Neoplasms / Q000382:microbiology / Q000473:pathology
D018829:Adhesins, Bacterial; C400429:BabA protein, Helicobacter pylori; C451032:BabB protein, Helicobacter pylori; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial