Prevalence of avian pathogenic Escherichia coli (APEC) clone harboring sfa gene in Brazil.
Escherichia coli sfa+ strains isolated from poultry were serotyped and characterized by polymerase chain reaction (PCR) and amplified fragment length polymorphism (AFLP). Isolates collected from 12 Brazilian poultry farms mostly belonged to serogroup O6, followed by serogroups O2, O8, O21, O46, O78, O88, O106, O111, and O143. Virulence genes associated were: iuc 90%, fim 86% neuS 60%, hly 34%, tsh 28%, crl/csg 26%, iss 26%, pap 18%, and 14% cnf. Strains from the same farm presented more than one genotypic pattern belonging to different profiles in AFLP. AFLP showed a clonal relation between Escherichia coli sfa+ serogroup O6. The virulence genes found in these strains reveal some similarity with extraintestinal E. coli (ExPEC), thus alerting for potential zoonotic risk.
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Avenue Prof. Dr. Orlando Marques de Paiva, 87 05508-900 São Paulo, SP, Brazil. tknobl@usp.br
Terezinha
T
Knöbl
Andrea Micke
AM
Moreno
Renata
R
Paixão
Tânia Aparecida Tardelli
TA
Gomes
Mônica Aparecida Midolli
MA
Vieira
Domingos
D
da Silva Leite
Jesus E
JE
Blanco
Antônio José Piantino
AJ
Ferreira
D000818:Animals; D001483:Base Sequence; D001938:Brazil; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011200:Poultry / Q000382:microbiology*; D014774:Virulence
D017931:DNA Primers; D029968:Escherichia coli Proteins; C101637:ymcE protein, E coli