Involvement of reactive oxygen species and glutathione in gallic acid-induced             human umbilical vein endothelial cell death.
Gallic acid (GA) has various biological effects including apoptosis. In             this study, we investigated the effects of GA on human primary umbilical vein             endothelial cells (HUVECs) in relation to cell growth, cell death, reactive oxygen             species (ROS) and glutathione (GSH). Treatment with 200 or 400 µM GA inhibited             the growth of HUVECs at 24 h and induced cell death, which was accompanied by             the loss of mitochondrial membrane potential (MMP; ∆Ψm). GA decreased or increased             ROS levels including O2•-. It dose-dependently increased GSH depleted cell numbers.             Pan-caspase inhibitor (Z-VAD) did not affect cell growth inhibition, cell death,             ROS and GSH levels in GA-treated HUVECs. However, N-acetyl-cysteine (NAC; a well             known antioxidant) and L-buthionine sulfoximine (BSO; an inhibitor of GSH synthesis)             enhanced cell growth inhibition, cell death and MMP (∆Ψm) loss in GA-treated HUVECs.             NAC decreased general ROS levels in GA-treated HUVECs, but strongly increased             O2•- levels in these cells. Both NAC and BSO intensified the GSH depletion of             GA-treated HUVECs. In conclusion, GA treatment induced growth inhibition and death             of HUVECs. The changes of ROS and GSH levels by NAC and BSO influenced cell growth             and death in GA-treated HUVECs.
Department of Physiology, Medical School, Research Institute for Endocrine Sciences Chonbuk National University, JeonJu 561-180, Republic of Korea. parkwh71@chonbuk.ac.kr
Woo Hyun
WH
Park
Suhn Hee
SH
Kim
D000111:Acetylcysteine / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005707:Gallic Acid / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*
D017382:Reactive Oxygen Species; D019328:Buthionine Sulfoximine; D005707:Gallic Acid; D005978:Glutathione; D000111:Acetylcysteine