Oxaloacetate and malate production in engineered Escherichia coli by expression of codon-optimized phosphoenolpyruvate carboxylase2 gene from Dunaliella salina.
A new phosphoenolpyruvate carboxylase (PEPC) gene of Dunaliella salina is identified using homology analysis was conducted using PEPC gene of Chlamydomonas reinhardtii and Arabidopsis thaliana. Recombinant E. coli SGJS115 with increased production of malate and oxaloacetate was developed by introducing codon-optimized phosphoenolpyruvate carboxylase2 (OPDSPEPC2) gene of Dunaliella salina. E. coli SGJS115 yielded a 9.9 % increase in malate production. In addition, E. coli SGJS115 exhibited two times increase in the yield of oxaloacetate over the E. coli SGJS114 having identified PEPC2 gene obtained from Dunaliella salina.
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea.
Soohyun
S
Park
Kwang Suk
KS
Chang
Eonseon
E
Jin
Seung Pil
SP
Pack
Jinwon
J
Lee
D003062:Codon / Q000235:genetics; D004926:Escherichia coli / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods; D008293:Malates / Q000302:isolation & purification / Q000378:metabolism*; D062907:Oxaloacetic Acid / Q000302:isolation & purification / Q000378:metabolism*; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000378:metabolism; D019657:Solanaceae / Q000201:enzymology* / Q000235:genetics*; D014162:Transfection / Q000379:methods
D003062:Codon; D008293:Malates; D011994:Recombinant Proteins; D062907:Oxaloacetic Acid; C030298:malic acid; D010730:Phosphoenolpyruvate Carboxylase