Inactivation of thioredoxin f1 leads to decreased light activation of ADP-glucose pyrophosphorylase and altered diurnal starch turnover in leaves of Arabidopsis plants.
Chloroplast thioredoxin f (Trx f) is an important regulator of primary metabolic enzymes. However, genetic evidence for its physiological importance is largely lacking. To test the functional significance of Trx f in vivo, Arabidopsis mutants with insertions in the trx f1 gene were studied, showing a drastic decrease in Trx f leaf content. Knockout of Trx f1 led to strong attenuation in reductive light activation of ADP-glucose pyrophosphorylase (AGPase), the key enzyme of starch synthesis, in leaves during the day and in isolated chloroplasts, while sucrose-dependent redox activation of AGPase in darkened leaves was not affected. The decrease in light-activation of AGPase in leaves was accompanied by a decrease in starch accumulation, an increase in sucrose levels and a decrease in starch-to-sucrose ratio. Analysis of metabolite levels at the end of day shows that inhibition of starch synthesis was unlikely due to shortage of substrates or changes in allosteric effectors. Metabolite profiling by gas chromatography-mass spectrometry pinpoints only a small number of metabolites affected, including sugars, organic acids and ethanolamine. Interestingly, metabolite data indicate carbon shortage in trx f1 mutant leaves at the end of night. Overall, results provide in planta evidence for the role played by Trx f in the light activation of AGPase and photosynthetic carbon partitioning in plants.
Department Biologie I, Metabolism Group Mass-Spectrometry Group, Ludwig-Maximilians-Universität München, Martinsried, Germany.
Ina
I
Thormählen
Joachim
J
Ruber
Edda
E
von Roepenack-Lahaye
Sven-Matthias
SM
Ehrlich
Vincent
V
Massot
Christine
C
Hümmer
Justyna
J
Tezycka
Emmanuelle
E
Issakidis-Bourguet
Peter
P
Geigenberger
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D054479:Chloroplast Thioredoxins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology; D002940:Circadian Rhythm; D004789:Enzyme Activation; D008401:Gas Chromatography-Mass Spectrometry; D055786:Gene Knockout Techniques; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000378:metabolism*; D008027:Light; D010084:Oxidation-Reduction; D010788:Photosynthesis; D018515:Plant Leaves / Q000378:metabolism*; D013213:Starch / Q000096:biosynthesis*; D013395:Sucrose / Q000378:metabolism
D054479:Chloroplast Thioredoxins; D013395:Sucrose; D013213:Starch; D051599:Glucose-1-Phosphate Adenylyltransferase