Regioselective synthesis of 1,3,5-adamantanetriol from 1,3-adamantanediol using Kitasatospora cells.
Washed cells (62 mg) of Kitasatospora sp. GF12 in 4 ml buffer (pH 7) catalyzed the regioselective hydroxylation of 60 mM 1,3-adamantanediol [1,3-ad(OH)(2)] to 30.9 mM 1,3,5-adamantanetriol [1,3,5-ad(OH)(3)] over 120 h at 24 °C. Glycerol at 400 mM was added to the reaction mixture to recycle the intracellular NADH/NADPH. Whole cells of GF12, also catalyzed the hydroxylation of 10 mM 1-adamantanol (1-adOH), to 3.6 mM 1,3,5-ad(OH)(3).
Department of Biomolecular Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. mitukura@gifu-u.ac.jp
Koichi
K
Mitsukura
Naoki
N
Yamanaka
Toyokazu
T
Yoshida
Toru
T
Nagasawa
D000218:Adamantane / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D005990:Glycerol / Q000378:metabolism; D006900:Hydroxylation; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D013237:Stereoisomerism; D013306:Streptomycetaceae / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D009243:NAD; D009249:NADP; D005990:Glycerol; D000218:Adamantane