Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen.
Perfluorooctane sulfonate (PFOS) is a pollutant widely found throughout nature and is toxic to animals. We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analysis using the FASTAskan program on the RELIC bioinformatics server showed several human proteins that likely bound PFOS. Among them, we confirmed binding between PFOS and a recombinant soluble form of monocyte differentiation antigen CD14 (sCD14) by a surface plasmon biosensor. Furthermore, PFOS inhibited TNF-α production induced by the sCD14 in mouse macrophage RAW264.7 cells.
Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Yuka
Y
Miyano
Senko
S
Tsukuda
Ippei
I
Sakimoto
Ryo
R
Takeuchi
Satomi
S
Shimura
Noriyuki
N
Takahashi
Tomoe
T
Kusayanagi
Yoichi
Y
Takakusagi
Mami
M
Okado
Yuki
Y
Matsumoto
Kaori
K
Takakusagi
Toshifumi
T
Takeuchi
Shinji
S
Kamisuki
Atsuo
A
Nakazaki
Keisuke
K
Ohta
Masahiko
M
Miura
Kouji
K
Kuramochi
Yoshiyuki
Y
Mizushina
Susumu
S
Kobayashi
Fumio
F
Sugawara
Kengo
K
Sakaguchi
D017738:Alkanesulfonic Acids / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D019295:Computational Biology; D005466:Fluorocarbons / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000378:metabolism; D051379:Mice; D019151:Peptide Library; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012984:Software; D020349:Surface Plasmon Resonance; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D017738:Alkanesulfonic Acids; D002352:Carrier Proteins; D005466:Fluorocarbons; D018950:Lipopolysaccharide Receptors; D019151:Peptide Library; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C076994:perfluorooctane sulfonic acid