Flavonoid aglycones can compete with Ochratoxin A for human serum albumin: a new possible mode of action.
The mycotoxin Ochratoxin A (OTA) appears worldwide in cereals, plant products, different foods and drinks. Ochratoxin A binds to plasma albumin with a very high affinity. However, it is well known that natural flavonoids can also bind to human serum albumin (HSA) at the same binding site as OTA does (site I, subdomain IIA). A few experimental literature data suggest that reducing the bound fraction of OTA speeds up its elimination rate with a potential decrease in its toxicity. In our experimental model competitive binding properties of flavonoid aglycones were examined with a fluorescence polarization based approach. Our data show that some of the flavonoids are able to remove the toxin from HSA. We conclude that among the 13 studied flavonoid aglycones galangin and quercetin were the most effective competitors for OTA.
Institute of Laboratory Medicine, University of Pécs, Pécs H-7624, Hungary.
Miklós
M
Poór
Sándor
S
Kunsági-Máté
Tímea
T
Bencsik
József
J
Petrik
Sanda
S
Vladimir-Knežević
Tamás
T
Kőszegi
D001667:Binding, Competitive; D005419:Flavonoids / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D009793:Ochratoxins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*
D005419:Flavonoids; D009793:Ochratoxins; D012709:Serum Albumin; C025589:ochratoxin A