Up-regulation of antioxidants in tobacco by low concentrations of H₂O₂ suppresses necrotic disease symptoms.
Pretreatment of tobacco leaves with low concentrations (5 to 10 mM) of H₂O₂ suppressed hypersensitive-type necrosis associated with resistance to Tobacco mosaic virus (TMV) or Pseudomonas syringae pv. phaseolicola. The same pretreatment resulted in suppression of normosensitive necrosis associated with susceptibility to Botrytis cinerea. This type of H₂O₂-mediated, induced disease symptom resistance correlated with enhanced host antioxidant capacity, i.e., elevated enzymatic activities of catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (POX) after viral and bacterial infections. Induction of genes that encode the antioxidants superoxide dismutase (SOD), CAT, and APX was also enhanced early after TMV infection. Artificial application of SOD and CAT suppressed necroses caused by viral, bacterial, or fungal pathogens similarly as H₂O₂ pretreatment, implying that H₂O₂-mediated symptom resistance operates through enhancement of plant antioxidant capacity. Pathogen multiplication was not significantly affected in H₂O₂-pretreated plants. Salicylic acid (SA), a central component of plant defense, does not seem to function in this type of H₂O₂-mediated symptom resistance, indicated by unchanged levels of free and bound SA and a lack of early up-regulation of an SA glucosyltransferase gene in TMV-infected H₂O₂-pretreated tobacco. Taken together, H₂O₂-mediated, induced resistance to necrotic symptoms in tobacco seems to depend on enhanced antioxidant capacity.
Plant Protection Institute, Centre for agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.
Yaser Mohamed
YM
Hafez
Renáta
R
Bacsó
Zoltán
Z
Király
András
A
Künstler
Lóránt
L
Király
D000975:Antioxidants / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology* / Q000821:virology; D018515:Plant Leaves / Q000187:drug effects / Q000382:microbiology / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011549:Pseudomonas / Q000145:classification; D018749:RNA, Plant / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014027:Tobacco Mosaic Virus; D015854:Up-Regulation
D000975:Antioxidants; D010940:Plant Proteins; D018749:RNA, Plant; D006861:Hydrogen Peroxide