Antioxidant activity of topiramate: an antiepileptic agent.
A number of experimental and clinical reports suggest the involvement of oxidative stress in pathophysiology of epilepsy. Topiramate, a new antiepileptic drug, induces antioxidant effect in epileptic animals. However, to date, no further studies appear to be carried out in order to demonstrate the ability of topiramate to act as antioxidant. Therefore, the objective of this work was to evaluate the in vitro superoxide (O₂(·-)), hydroxyl radical (OH·), hypochlorous acid (HOCl), hydrogen peroxide (H₂O₂), singlet oxygen ((1)O₂) and peroxynitrite (ONOO(-)) scavenging capacity of topiramate in comparison with reference compounds. In addition, we investigated the possible antitumour activity of this compound in some cancer cell lines. Topiramate displays a scavenging capacity compared to the reference compound, with the exception of ONOO(-), although it was less efficient than nordihydroguaiaretic acid, dimethylthiourea, ascorbic acid, sodium pyruvate and glutathione for O₂(·-), OH·, HOCl, H₂O₂ and (1)O₂(P < 0.0001), respectively, and not induced significant growth inhibition in cancer cell lines. The direct antioxidant properties of topiramate could explain the neuroprotective effects attributed to this compound and suggest its use as chemopreventive agent in a future.
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría, 04530 Mexico, DF, Mexico. noemicr2001@yahoo.com.mx
N
N
Cárdenas-Rodríguez
E
E
Coballase-Urrutia
B
B
Huerta-Gertrudis
M E
ME
García-Cruz
J
J
Pedraza-Chaverri
R
R
Coria-Jiménez
C
C
Bandala
M
M
Ruíz-García
D000975:Antioxidants / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000145:classification / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000378:metabolism*; D005632:Fructose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D010084:Oxidation-Reduction / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077236:Topiramate
D000975:Antioxidants; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D000077236:Topiramate; D005632:Fructose