Killer cell immunoglobulin-like receptors (KIR) typing by DNA sequencing.
DNA sequencing is a powerful technique for identifying allelic variation within the natural killer cell immunoglobulin-like receptor genes. Because of the relatively large size of the KIR genes, each locus is amplified in two or more overlapping segments. Sanger sequencing of each gene from a preparation containing one or two alleles yields a sequence that is used to identify the alleles by comparison with a reference database.
Department of Pediatrics, CW Bill Young Marrow Donor Recruitment and Research Program, Georgetown University Medical Centre, Washington, DC, USA.
Lihua
L
Hou
Minghua
M
Chen
Noriko
N
Steiner
Kanthi
K
Kariyawasam
Jennifer
J
Ng
Carolyn K
CK
Hurley
D006801:Humans; D010641:Phenotype; D054340:Receptors, KIR / Q000235:genetics*; D017422:Sequence Analysis, DNA
D054340:Receptors, KIR