Analysis of F9 point mutations and their correlation to severity of haemophilia B disease.
Haemophilia B is an X-linked recessive disorder caused by deficiency of functional coagulation factor IX, which results almost exclusively from mutations in the F9 gene. We sought to determine features, which could distinguish between mutations that cause severe disease symptoms from those that cause non-severe disease symptoms. Towards this objective, we have performed a statistical analysis of reported point mutations in F9. These include: potential local changes in mRNA free energy, codon usage, charge and type of mutated amino acid, location of the mutation with regard to protein secondary structure and functional domain and amino acids' evolutionary conservation scores. Wilcoxon signed-rank tests showed highly significant differences between severe and non-severe disease causing mutations in their effect on free energy of small mRNA fragments and evolutionarily conserved amino acids. Our results suggest that information at the mRNA level as well as conservation of the amino acid correlate well with disease severity. This study demonstrates that computational tools may be used to characterize the severity of haemophilia B associated with point mutations and suggests their utility in predicting the outcome of sequence changes in recombinant proteins.
Division of Hematology, Laboratory of Hemostasis, Center for Biologics Evaluation & Research, US FDA, Bethesda, MD 20892, USA.
N
N
Hamasaki-Katagiri
R
R
Salari
V L
VL
Simhadri
S C
SC
Tseng
E
E
Needlman
N C
NC
Edwards
Z E
ZE
Sauna
V
V
Grigoryan
A A
AA
Komar
T M
TM
Przytycka
C
C
Kimchi-Sarfaty
D000596:Amino Acids / Q000737:chemistry; D020134:Catalytic Domain; D030541:Databases, Genetic; D005164:Factor IX / Q000235:genetics*; D002836:Hemophilia B / Q000235:genetics*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D017354:Point Mutation; D021382:Protein Sorting Signals; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D012720:Severity of Illness Index; D013816:Thermodynamics
D000596:Amino Acids; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D005164:Factor IX