Evaluation of melanogenesis in A-375 cells in the presence of DMSO and analysis of pyrolytic profile of isolated melanin.
The increase of a skin malignant melanoma (melanoma malignum) incidence in the world has been observed in recent years. The tumour, especially in advanced stadium with metastases, is highly resistant to conventional treatment. One of the strategies is to modulate melanogenesis using chemical compounds. In this study, the processes of differentiation and melanogenesis induced by dimethylsulfoxide (DMSO) in human melanoma cells (A-375) were investigated. Natural melanin isolated from A-375 melanoma cell line treated with 0.3% DMSO was analyzed by pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS) method. The products derived from pheomelanin have not been stated in the pyrolytic profile of analyzed melanin. Within all products derived from eumelanins, 1,2-benzenediol has been predominated. It has been shown that in the melanoma cells stimulated with 0.3% and 1% DMSO, the increase of transcriptional activity of the tyrosinase gene took place. It was accompanied by the rise of tyrosinase activity and an accumulation of melanin in the cells. The better knowledge about the structure of melanins can contribute to establish the uniform criteria of malignant melanoma morbidity risk.
Department of Biopharmacy, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland. echodurek@sum.edu.pl
Ewa
E
Chodurek
Arkadiusz
A
Orchel
Joanna
J
Orchel
Sławomir
S
Kurkiewicz
Natalia
N
Gawlik
Zofia
Z
Dzierżewicz
Krystyna
K
Stępień
D045744:Cell Line, Tumor; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008543:Melanins / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism; D012878:Skin Neoplasms
D008543:Melanins; D014442:Monophenol Monooxygenase