pKa modulation of the acid/base catalyst within GH32 and GH68: a role in substrate/inhibitor specificity?
Glycoside hydrolases of families 32 (GH32) and 68 (GH68) belong to clan GH-J, containing hydrolytic enzymes (sucrose/fructans as donor substrates) and fructosyltransferases (sucrose/fructans as donor and acceptor substrates). In GH32 members, some of the sugar substrates can also function as inhibitors, this regulatory aspect further adding to the complexity in enzyme functionalities within this family. Although 3D structural information becomes increasingly available within this clan and huge progress has been made on structure-function relationships, it is not clear why some sugars bind as inhibitors without being catalyzed. Conserved aspartate and glutamate residues are well known to act as nucleophile and acid/bases within this clan. Based on the available 3D structures of enzymes and enzyme-ligand complexes as well as docking simulations, we calculated the pKa of the acid-base before and after substrate binding. The obtained results strongly suggest that most GH-J members show an acid-base catalyst that is not sufficiently protonated before ligand entrance, while the acid-base can be fully protonated when a substrate, but not an inhibitor, enters the catalytic pocket. This provides a new mechanistic insight aiming at understanding the complex substrate and inhibitor specificities observed within the GH-J clan. Moreover, besides the effect of substrate entrance on its own, we strongly suggest that a highly conserved arginine residue (in the RDP motif) rather than the previously proposed Tyr motif (not conserved) provides the proton to increase the pKa of the acid-base catalyst.
Laboratory of Molecular Plant Physiology, Institute of Botany and Microbiology, KU Leuven, Heverlee, Belgium.
Shuguang
S
Yuan
Katrien
K
Le Roy
Tom
T
Venken
Willem
W
Lammens
Wim
W
Van den Ende
Marc
M
De Maeyer
D000596:Amino Acids / Q000737:chemistry; D002384:Catalysis; D005630:Fructans / Q000737:chemistry / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008968:Molecular Conformation; D056004:Molecular Dynamics Simulation; D009154:Mutation; D011485:Protein Binding; D013379:Substrate Specificity; D013395:Sucrose / Q000737:chemistry / Q000378:metabolism
D000596:Amino Acids; D005630:Fructans; D013395:Sucrose; D006026:Glycoside Hydrolases; C023086:inulinase