Simple sets for digital microphotography used and tested in the study of microorganisms.
Four simple sets for digital microphotography are described that have been tested with the Carl Zeiss Jena, Meopta Prague, Lambda Prague, and LOMO Sankt Petersburg microscopes and with DSLR Nikon D 70 and Nikon D 300 cameras. They permit precise image focusing in the camera using a prism Zeiss. The sets make use of commonly available extensions Zeiss, Praktica and reductions Nikon-Praktica manufactured by ROWI (without a lens) or HAMA (with a lens). An extension has further been designed and manufactured for connecting the DSLR Nikon D 300 camera fitted with the HAMA reduction (only with a lens) and a focusing extensible prism with Zeiss Jena light measurement. It permits a precise image focusing of low light intensity objects (autofluorescence or low-contrast or moving objects when using positive or negative phase contrast). The sets are applicable to all microscopes constructed according to German DIN industrial standards.
Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. zizka@biomed.cas.cz
Zdeněk
Z
Zižka
D007091:Image Processing, Computer-Assisted / Q000379:methods; D008828:Microbiological Techniques / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D010781:Photography / Q000379:methods*; D014871:Water Microbiology; D014934:Wood / Q000382:microbiology