Dopamine-assisted rapid fabrication of nanoscale protein arrays by colloidal lithography.
The development of cost-effective methodologies for the precise nanometer-scale positioning of biomolecules permits the low-cost production of various biofunctional devices for a range of biomedical and nanotechnological applications. By combining colloidal lithography and the mussel-inspired multifunctional polydopamine coating, we present a novel parallel benchtop method that allows rapid nanoscale patterning of proteins without the need for electrically powered equipment in the fabrication process. The PDA-immobilized binary nanopattern consisting of BSA surrounded by PLL-g-PEG is fabricated over a large area, and the integrity of the pattern is confirmed using AFM and FM.
Interdisciplinary Nanoscience Center, Faculty of Science, Aarhus University, Ny Munkegade, 8000 Aarhus C, Denmark. ryo@inano.au.dk
Ryosuke
R
Ogaki
Dines T
DT
Bennetsen
Ilko
I
Bald
Morten
M
Foss
D003102:Colloids; D004298:Dopamine / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry; D011327:Printing / Q000379:methods*; D040081:Protein Array Analysis; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
D003102:Colloids; D011506:Proteins; C443714:polylysine-graft-(poly(ethylene glycol)); D011107:Polylysine; D011092:Polyethylene Glycols; D004298:Dopamine