Identification and characterization of FHL3 as a novel angiogenin-binding partner.
Angiogenin (Ang) is known to induce cell proliferation and inhibit apoptosis by cellular signaling pathways and by direct nuclear functions of Ang, but the mechanism of action for Ang is not yet clear. The aim of present study was to identify novel binding partner of Ang and to explore the underlying mechanism. With the use of yeast two-hybrid screening system, Ang was used as the bait to screen human fetal hepatic cDNA library for interacting proteins. Four and a half LIM domains 3 (FHL3) was identified as a novel Ang binding partner. The interaction between Ang and the full length FHL3 was further confirmed by yeast two-hybrid assay, co-immunoprecipitation and GST pull-down assays. Furthermore, FHL3 was required for Ang-mediated HeLa cell proliferation and nuclear translocation of Ang. These findings suggest that the interaction between Ang and FHL3 may provide some clues to the mechanisms of Ang-regulated cell growth and apoptosis.
Laboratory of Genome Engineering, Beijing Institute of Basic Medical Sciences, Beijing, China.
Wenrong
W
Xia
Wenliang
W
Fu
Ling
L
Cai
Haibo
H
Kong
Xin
X
Cai
Jing
J
Liu
Yuanyuan
Y
Wang
Minji
M
Zou
Donggang
D
Xu
D001483:Base Sequence; D017931:DNA Primers; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012259:Ribonuclease, Pancreatic / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D017931:DNA Primers; C117563:FHL3 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; C046366:angiogenin; D012259:Ribonuclease, Pancreatic