P4-ATPase ATP8A2 acts in synergy with CDC50A to enhance neurite outgrowth.
P(4)-ATPases are lipid flippases that transport phospholipids across cellular membranes, playing vital roles in cell function. In humans, the disruption of the P(4)-ATPase ATP8A2 gene causes a severe neurological phenotype. Here, we found that Atp8a2 mRNA was highly expressed in PC12 cells, hippocampal neurons and the brain. Overexpression of ATP8A2 increased the length of neurite outgrowth in NGF-induced PC12 cells and in primary cultures of rat hippocampal neurons. Inducing the loss of function of CDC50A in hippocampal neurons via RNA interference reduced neurite outgrowth, and the co-overexpression of CDC50A and ATP8A2 in PC12 cells enhanced NGF-induced neurite outgrowth. These results indicate that ATP8A2, acting in synergy with CDC50A, performs an important role in neurite outgrowth in neurons.
State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences (SIBS), Graduate School of the Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China.
Qin
Q
Xu
Guo-Ying
GY
Yang
Na
N
Liu
Peng
P
Xu
Yue-Lei
YL
Chen
Zheng
Z
Zhou
Zhen-Ge
ZG
Luo
Xiaoyan
X
Ding
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D057809:HEK293 Cells; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D016501:Neurites / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D016716:PC12 Cells; D049294:Phospholipid Transfer Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats
D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C545244:Tmem30a protein, rat; D020932:Nerve Growth Factor; D000251:Adenosine Triphosphatases; C575049:ATP8A2 protein, rat