Arabidopsis-derived shrimp viral-binding protein, PmRab7 can protect white spot syndrome virus infection in shrimp.
White spot syndrome virus is currently the leading cause of production losses in the shrimp industry. Penaeus monodon Rab7 protein has been recognized as a viral-binding protein with an efficient protective effect against white spot syndrome infection. Plant-derived recombinant PmRab7 might serve as an alternative source for in-feed vaccination, considering the remarkable abilities of plant expression systems. PmRab7 was introduced into the Arabidopsis thaliana T87 genome. Arabidopsis-derived recombinant PmRab7 showed high binding activity against white spot syndrome virus and a viral envelope, VP28. The growth profile of Arabidopsis suspension culture expressing PmRab7 (ECR21# 35) resembled that of its counterpart. PmRab7 expression in ECR21# 35 reached its maximum level at 5 mg g(-1) dry weight in 12 days, which was higher than those previously reported in Escherichia coli and in Pichia. Co-injection of white spot syndrome virus and Arabidopsis crude extract containing PmRab7 in Litopenaeus vannamei showed an 87% increase in shrimp survival rate at 5 day after injection. In this study, we propose an alternative PmRab7 source with higher production yield, and cheaper culture media costs, that might serve the industry's need for an in-feed supplement against white spot syndrome infection.
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Chonprakun
C
Thagun
Jiraporn
J
Srisala
Kallaya
K
Sritunyalucksana
Jarunya
J
Narangajavana
Punchapat
P
Sojikul
D000704:Analysis of Variance; D000818:Animals; D017756:Aquaculture / Q000379:methods; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D019149:Bioreactors; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D017931:DNA Primers / Q000235:genetics; D019587:Dietary Supplements; D004797:Enzyme-Linked Immunosorbent Assay; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D009500:Neutralization Tests; D033561:Penaeidae / Q000276:immunology / Q000821:virology*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015996:Survival Rate; D046848:White spot syndrome virus 1 / Q000276:immunology / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D000090802:rab7 GTP-Binding Proteins
D017931:DNA Primers; D011994:Recombinant Proteins; D000090802:rab7 GTP-Binding Proteins; D020691:rab GTP-Binding Proteins