Sphingobacterium psychroaquaticum sp. nov., a psychrophilic bacterium isolated from Lake Michigan water.
A psychrophilic, Gram-negative bacterium, designated MOL-1(T), was isolated from water of Lake Michigan. 16S rRNA gene sequence analysis revealed that the sequence of strain MOL-1(T) has sequence similarity of 95.6, 94.8, 94.3, 94.3, 94.2 and 93.9 %, respectively, to the 16S rRNA gene sequences of Sphingobacterium shayense HS39(T), S. lactis WCC 4512(T), S. composti T5-12(T), S. daejeonense TR6-04(T), S. bambusae IBFC2009(T) and S. alimentarium WCC 4521(T). The major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c). Menaquinone MK-7 is the predominant respiratory quinone, while sym-homospermidine is the predominant polyamine. The polar lipid profile is composed of the predominant lipids phosphatidylethanolamine and unidentified polar lipid L2, with moderate amounts of unidentified polar lipids L1, L5 and L6 and unidentified aminophospholipids APL1 and APL2 and minor to trace amounts of unidentified polar lipids L3, L4, L7, L8, L9 and L10, unidentified phospholipid PL4 and unidentified aminophospholipid APL3. After molecular and phenotypic studies, including chemotaxonomic analyses, it was concluded that strain MOL-1(T) represents a novel Sphingobacterium species, for which the name Sphingobacterium psychroaquaticum sp. nov. is proposed. The type strain is MOL-1(T) ( = NRRL B-59232(T)  = DSM 22418(T)).
Water Quality Center, Marquette University, Civil & Environmental Engineering, PO Box 1881 Milwaukee, WI 53233, USA.|Kleen Test Products, 1611 Sunset Road, Port Washington, WI 53074, USA.
Richard A
RA
Albert
Kleen Test Products, 1611 Sunset Road, Port Washington, WI 53074, USA.
Nancy E
NE
Waas
Kleen Test Products, 1611 Sunset Road, Port Washington, WI 53074, USA.
Shawn C
SC
Pavlons
Kleen Test Products, 1611 Sunset Road, Port Washington, WI 53074, USA.
Jamie L
JL
Pearson
Department of Biological Sciences, UW-Milwaukee, Milwaukee, WI 53201, USA.|Kleen Test Products, 1611 Sunset Road, Port Washington, WI 53074, USA.
Laura
L
Ketelboeter
Grup de Microbiologia Marina, Institut Mediterrani d'Estudis Avançats, Mallorca, E-07190 Esporles, Spain.
Ramon
R
Rosselló-Móra
Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna, Austria.
Hans-Jürgen
HJ
Busse
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000031:analogs & derivatives / Q000032:analysis; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; C007961:sym-homospermidine; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7; D013095:Spermidine