In vitro and in vivo isolation and characterization of Duvenhage virus.
A fatal human case of Duvenhage virus (DUVV) infection in a Dutch traveller who had returned from Kenya was reported in 2007. She exhibited classical symptoms of rabies encephalitis with distinct pathological findings. In the present study we describe the isolation and characterization of DUVV in vitro and its passage in BALB/c mice. The virus proved to be neuroinvasive in both juvenile and adult mice, resulting in about 50% lethality upon peripheral infection. Clinical signs in infected mice were those of classical rabies. However, the distribution of viral antigen expression in the brain differed from that of classical rabies virus infection and neither inclusion bodies nor neuronal necrosis were observed. This is the first study to describe the in vitro and in vivo isolation and characterization of DUVV.
Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands. p.koraka@erasmusmc.nl
Penelope
P
Koraka
Byron E E
BE
Martina
Jouke M
JM
Roose
Pieter-Paul A M
PP
van Thiel
Geert
G
van Amerongen
Thijs
T
Kuiken
Albert D M E
AD
Osterhaus
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D001921:Brain / Q000473:pathology / Q000821:virology; D045744:Cell Line, Tumor; D006224:Cricetinae; D018792:Encephalitis, Viral / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D005260:Female; D006801:Humans; D018114:Lyssavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D017423:Sequence Analysis, RNA; D012692:Serial Passage; D014195:Travel
D000956:Antigens, Viral; D012367:RNA, Viral