Effect of graphene oxide on undifferentiated and retinoic acid-differentiated SH-SY5Y cells line.
Graphene oxide (GO), has created an unprecedented opportunity for development and application in biology, due to its abundant functional groups and well water solubility. Recently, the potential toxicity of GO in the environment and in humans has garnered more and more attention. In this paper, we systematically studied the cytotoxicity of GO nanosheets via examining the effect of GO on the morphology, viability and differentiation of a human neuroblastoma SH-SY5Y cell line, which was an ideal model used to study neuronal disease in vitro. The results suggested that GO had no obvious cytotoxicity at low concentration (<80 μg mL(-1)) for 96 h, but the viability of cells exhibited dose- and time-dependent decreases at high concentration (≥ 80 μg mL(-1)). Moreover, GO did not induce apoptosis. Very interestingly, GO significantly enhanced the differentiation of SH-SY5Y induced-retinoic acid (RA) by evaluating neurite length and the expression of neuronal marker MAP2. These data provide a promising application for neurodegenerative diseases.
Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
Min
M
Lv
Yujie
Y
Zhang
Le
L
Liang
Min
M
Wei
Wenbing
W
Hu
Xiaoming
X
Li
Qing
Q
Huang
D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006108:Graphite / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D010087:Oxides / Q000737:chemistry*; D014212:Tretinoin / Q000737:chemistry* / Q000633:toxicity
C489788:MAP2 protein, human; D008869:Microtubule-Associated Proteins; D010087:Oxides; D014212:Tretinoin; D006108:Graphite