The comet assay for the evaluation of genotoxic potential of landfill leachate.
Genotoxic assessment of landfill leachate before and after biological treatment was conducted with two human cell lines (Me45 and NHDF) and Daphnia magna somatic cells. The alkali version of comet assay was used to examine genotoxicity of leachate by DNA strand breaks analysis and its repair dynamics. The leachate samples were collected from Zabrze landfill, situated in the Upper Silesian Industrial District, Poland. Statistically significant differences (Kruskal-Wallice ANOVA rank model) were observed between DNA strand breaks in cells incubated with leachate before and after treatment (P < 0.001). Nonparametric Friedman ANOVA confirmed time-reliable and concentration-reliable cells response to leachate concentration. Examinations of chemical properties showed a marked decrease in leachate parameters after treatment which correlate to reduced genotoxicity towards tested cells. Obtained results demonstrate that biological cotreatment of leachate together with municipal wastewater is an efficient method for its genotoxic potential reduction; however, treated leachate still possessed genotoxic character.
Institute of Water and Wastewater Engineering, Silesian University of Technology, Akademicka Street 2A, 44-100 Gliwice, Poland.
Kamila
K
Widziewicz
Joanna
J
Kalka
Magdalena
M
Skonieczna
Paweł
P
Madej
D000818:Animals; D002460:Cell Line; D020552:Comet Assay; D003621:Daphnia; D060587:Groundwater; D006801:Humans; D009153:Mutagens / Q000633:toxicity*; D011044:Poland; D014873:Water Pollutants / Q000633:toxicity*
D009153:Mutagens; D014873:Water Pollutants