Multifunctional poly (lactide-co-glycolide) nanoparticles for luminescence/magnetic resonance imaging and photodynamic therapy.
Poly (lactide-co-glycolide) (PLGA) coupled with methoxy poly (ethylene glycol) (mPEG) or chlorin e6 (Ce6) was synthesized using the Steglich esterification method. PLGA-linked mPEG (PLGA-mPEG), PLGA-linked Ce6 (PLGA-Ce6), and Fe(3)O(4) were utilized to constitute multifunctional PLGA nanoparticles (∼160 nm) via the multi-emulsion W(1)/O/W(2) (water-in-oil-in-water) method. The photo-sensitizing properties of Ce6 molecules anchored to PLGA nanoparticles enabled in vivo luminescence imaging and photodynamic therapy for the tumor site. The encapsulation of Fe(3)O(4) allowed high contrast magnetic resonance (MR) imaging of the tumor in vivo. Overall, PLGA nanoparticles resulted in a significant tumor volume regression for the light-illuminated KB tumor in vivo and enhanced the contrast at the tumor region, compared to that of Feridex(®) (commercial contrast agent).
Division of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok 2-dong, Wonmi-gu, Bucheon-si, Gyeonggi-do 420-743, Republic of Korea.
Dong Jin
DJ
Lee
Ga Young
GY
Park
Kyung Taek
KT
Oh
Nam Muk
NM
Oh
Dong Sup
DS
Kwag
Yu Seok
YS
Youn
Young Taik
YT
Oh
Jin Woo
JW
Park
Eun Seong
ES
Lee
D000818:Animals; D045744:Cell Line, Tumor; D002735:Chlorophyllides; D003911:Dextrans; D005260:Female; D005290:Ferric Compounds; D006801:Humans; D007624:KB Cells; D019344:Lactic Acid / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000628:therapy; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000737:chemistry; D011091:Polyesters; D011092:Polyethylene Glycols / Q000737:chemistry; D011098:Polyglactin 910 / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011166:Porphyrins
D002735:Chlorophyllides; D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D017319:Photosensitizing Agents; D011091:Polyesters; D011166:Porphyrins; C558447:methoxypolyethyleneglycol-poly(lactic-co-glycolic acid); C000499:ferric oxide; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011098:Polyglactin 910; D011092:Polyethylene Glycols; C062985:phytochlorin; C065507:ferumoxides