Dorsal and ventral stimuli in cell-material interactions: effect on cell morphology.
Cells behave differently between bidimensional (2D) and tridimensional (3D) environments. While most of the in vitro cultures are 2D, most of the in vivo extracellular matrices are 3D, which encourages the development of more relevant culture conditions, seeking to provide more physiological models for biomedicine (e.g., cancer, drug discovery and tissue engineering) and further insights into any dimension-dependent biological mechanism. In this study, cells were cultured between two protein coated surfaces (sandwich-like culture). Cells used both dorsal and ventral receptors to adhere and spread, undergoing morphological changes with respect to the 2D control. Combinations of fibronectin and bovine serum albumin on the dorsal and ventral sides led to different cell morphologies, which were quantified from bright field images by calculating the spreading area and circularity. Although the mechanism underlying these differences remains to be clarified, excitation of dorsal receptors by anchorage to extracellular proteins plays a key role on cell behavior. This approach--sandwich-like culture--becomes therefore a versatile method to study cell adhesion in well-defined conditions in a quasi 3D environment.
Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, Spain.
José
J
Ballester-Beltrán
Myriam
M
Lebourg
Patricia
P
Rico
Manuel
M
Salmerón-Sánchez
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D048430:Cell Shape; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013499:Surface Properties
D005353:Fibronectins; D012710:Serum Albumin, Bovine