Laser capture.
This chapter describes detailed methods used for laser capture microdissection (LCM) of discrete subpopulations of cells. Topics covered include preparing tissue blocks, cryostat sectioning, processing slides, performing the LCM, and purification of RNA from LCM samples. Notes describe the fine points of each operation, which can often mean the difference between success and failure.
Division of Developmental Biology, Children's Hospital Medical Center, Cincinnati, OH, USA. steve.potter@cchmc.org
S Steven
SS
Potter
Eric W
EW
Brunskill
D000818:Animals; D016616:Cryoultramicrotomy / Q000379:methods; D004867:Equipment Design; D007668:Kidney / Q000196:embryology*; D061346:Laser Capture Microdissection / Q000295:instrumentation / Q000379:methods*; D012313:RNA / Q000302:isolation & purification
D012313:RNA