An efficient in vitro propagation protocol of cocoyam [Xanthosoma sagittifolium (L) Schott].
Sprouted corm sections of "South Dade" white cocoyam were potted and maintained in a greenhouse for 8 weeks. Shoot tips of 3-5 mm comprising the apical meristem with 4-6 leaf primordial, and approximately 0.5 mm of corm tissue at the base. These explants were treated to be used into the culture medium. A modified Gamborg's B5 mineral salts supplemented with 0.05 μM 1-naphthaleneacetic acid (NAA) were used throughout the study. Thidiazuron (TDZ) solution containing 0.01% dimethyl sulfoxide (DMSO) was used. Erlenmeyer flasks and test tubes were used for growing cultures. The effect of different media substrate, thidiazuron, and the interaction between TDZ and Benzylaminopurine (BAP) on cocoyam culture were tested. Results indicated that cocoyam can be successfully micropropagated in vitro through various procedures. All concentrations tested (5-20 μM BAP and 1-4 μM TDZ) produced more axillary shoots per shoot tip than the control without cytokinins. Greater proliferation rates were obtained through the use of 20 μM BAP and 2 μM TDZ, respectively, 12 weeks from initiation. Shoots produced with BAP were larger and more normal in appearance than those produced with TDZ, which were small, compressed, and stunted. The use of stationary liquid media is recommended for economic reasons.
Department of Horticulture & Landscape Architecture, Fort Collins, CO 80523-1173, USA.
Anne E
AE
Sama
Harrison G
HG
Hughes
Mohamed S
MS
Abbas
Mohamed A
MA
Shahba
D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D066298:In Vitro Techniques; D009280:Naphthaleneacetic Acids / Q000737:chemistry; D018520:Plant Shoots / Q000254:growth & development; D031063:Xanthosoma / Q000378:metabolism*
D003470:Culture Media; D009280:Naphthaleneacetic Acids; D004121:Dimethyl Sulfoxide