High-resolution melt PCR analysis for rapid identification of Chlamydia abortus live vaccine strain 1B among C. abortus strains and field isolates.
We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus live vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.
Bacterial Zoonoses Unit, French Agency for Food, Environmental & Occupational Health Safety (Anses), Maisons-Alfort, France.
Fabien
F
Vorimore
Noémie
N
Cavanna
Nadia
N
Vicari
Simone
S
Magnino
Hermann
H
Willems
Annie
A
Rodolakis
Victoria I
VI
Siarkou
Karine
K
Laroucau
D000818:Animals; D001428:Bacterial Vaccines / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D002689:Chlamydia / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D002690:Chlamydia Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D004269:DNA, Bacterial / Q000235:genetics; D015511:Goat Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D006041:Goats; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D044366:Transition Temperature; D062690:Vaccines, Live, Unattenuated / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D062690:Vaccines, Live, Unattenuated