Characterization of fluorescent poly(isobutylcyanoacrylate) nanoparticles obtained by copolymerization of a fluorescent probe during Redox Radical Emulsion Polymerization (RREP).
OBJECTIVE
The purpose of the work was to demonstrate that a polymerizable fluorescent labeled was incorporated in the core of chitosan/pluronic® F68-coated Poly(IsobutylCyanoAcrylate) (PIBCA) nanoparticles thanks to a covalent linkage. It was also aimed to show that the labeling did not modify the complement activation capacity of the nanoparticles which are designed as drug carriers for the in vivo delivery of siRNA.


METHODS
Fluorescent nanoparticles were prepared by adding a fluorescent monomer dye, methacryloxyethyl thiocarbamoyl rhodamine B during the preparation of nanoparticles by redox radical emulsion polymerization. The structure and composition of the fluorescent nanoparticles was investigated. The capacity of the fluorescent nanoparticles to activate the complement system was evaluated by 2D immunoelectrophoresis.


RESULTS
Results from the analysis of the composition and structure of polymers forming the nanoparticles showed that the fluorescent dye was incorporated in the core of the nanoparticles by formation of a stable covalent linkage with PIBCA. The labeled nanoparticles showed the same surface properties as the corresponding non-labeled nanoparticles based on analysis of the polymer structure, physicochemical properties and evaluation of their capacity to activate the complement system.


CONCLUSIONS
This work showed that the fluorescent PIBCA nanoparticles were labeled by incorporation of the fluorescent probe in the nanoparticle core and that the fluorescent probe did not modify the nanoparticle surface properties. These fluorescent nanoparticles can be proposed as relevant models to investigate how they deliver siRNA to their biological target in cell cultures and during in vivo experiments.
Pharmacotechnie, Biopharmacie, Université de Paris Sud, Chatenay Malabry, France.
Christelle
C
Zandanel
Christine
C
Vauthier
D048271:Chitosan / Q000737:chemistry*; D003167:Complement Activation / Q000187:drug effects; D003487:Cyanoacrylates / Q000737:chemistry*; D016503:Drug Delivery Systems; D004655:Emulsions; D004659:Enbucrilate; D005456:Fluorescent Dyes / Q000737:chemistry*; D007123:Immunoelectrophoresis, Two-Dimensional; D053758:Nanoparticles; D020442:Poloxamer / Q000737:chemistry*; D058105:Polymerization; D034741:RNA, Small Interfering / Q000008:administration & dosage; D012235:Rhodamines / Q000737:chemistry; D013499:Surface Properties
D003487:Cyanoacrylates; D004655:Emulsions; D005456:Fluorescent Dyes; D034741:RNA, Small Interfering; D012235:Rhodamines; D020442:Poloxamer; D048271:Chitosan; D004659:Enbucrilate