Prediction and identification of potential immunodominant epitopes in glycoproteins B, C, E, G, and I of herpes simplex virus type 2.
Twenty B candidate epitopes of glycoproteins B (gB2), C (gC2), E (gE2), G (gG2), and I (gI2) of herpes simplex virus type 2 (HSV-2) were predicted using DNAstar, Biosun, and Antheprot methods combined with the polynomial method. Subsequently, the biological functions of the peptides were tested via experiments in vitro. Among the 20 epitope peptides, 17 could react with the antisera to the corresponding parent proteins in the EIA tests. In particular, five peptides, namely, gB2(466-473) (EQDRKPRN), gC2(216-223) (GRTDRPSA), gE2(483-491) (DPPERPDSP), gG2(572-579) (EPPDDDDS), and gI2(286-295) (CRRRYRRPRG) had strong reaction with the antisera. All conjugates of the five peptides with the carrier protein BSA could stimulate mice into producing antibodies. The antisera to these peptides reacted strongly with the corresponding parent glycoproteins during the Western Blot tests, and the peptides reacted strongly with the antibodies against the parent glycoproteins during the EIA tests. The antisera against the five peptides could neutralize HSV-2 infection in vitro, which has not been reported until now. These results suggest that the immunodominant epitopes screened using software algorithms may be used for virus diagnosis and vaccine design against HSV-2.
Department of Biochemistry and Molecular Biology, School of Preclinical Medicine, Nanjing Medical University, Nanjing 210029, China.
Mingjie
M
Pan
Xingsheng
X
Wang
Jianmin
J
Liao
Dengke
D
Yin
Suqin
S
Li
Ying
Y
Pan
Yao
Y
Wang
Guangyan
G
Xie
Shumin
S
Zhang
Yuexi
Y
Li
D000465:Algorithms; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D019295:Computational Biology; D018604:Epitope Mapping; D006558:Herpes Genitalis / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D051379:Mice; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology; D014765:Viral Vaccines
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D016056:Immunodominant Epitopes; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C044525:glycoprotein B, herpes simplex virus type 2; C048232:glycoprotein C, herpes simplex virus type 2; C048621:glycoprotein E, herpes simplex-virus type 2; C045464:glycoprotein G, herpes simplex virus type 2