Protective effect of l-citrulline against ethanol-induced gastric ulcer in rats.
We examined the protective effect of l-citrulline on ethanol-induced gastric ulcer in rats. Administration of l-citrulline at doses of 300, 600 and 900mg/kg body weight prior to ethanol ingestion protected the stomach from ulceration. The gastric lesions were significantly attenuated by all doses of l-citrulline as compared to the ethanol group. Pre-treatment with l-citrulline prevented the oxidative damage and the decrease of nitric oxide content as well as the increase of the myeloperoxidase activity. Consequently, significant changes observed included the attenuation in the elevation in total nitric oxide synthase activity and inducible nitric oxide synthase activity as well as the decrease in constitutive nitric oxide synthase activity in the gastric mucosa induced by ethanol. Analysis of serum cytokines of ethanol-induced rats showed a moderate decrease in interleukin-10 with considerable increase of interleukin-6 while l-citrulline inhibited the acute alteration of cytokines. These results suggested the gastroprotective effect of l-citrulline.
Department of Pharmacy, Xuzhou Medical College, Jiangsu, China. Electronic address: cbpe201087@gmail.com.
Yi
Y
Liu
Department of Pharmacy, Xuzhou Medical College, Jiangsu, China. Electronic address: xuezhetian@163.com.
Xia
X
Tian
Department of Pharmacy, Xuzhou Medical College, Jiangsu, China. Electronic address: jifeng0241527@163.com.
Lingshan
L
Gou
Department of Pharmacy, Xuzhou Medical College, Jiangsu, China. Electronic address: cbpe201104@163.com.
Xiaobin
X
Fu
Department of Pharmacy, Xuzhou Medical College, Jiangsu, China. Electronic address: lisai0821@sina.com.
Sai
S
Li
Department of Pharmacy, Xuzhou Medical College, Jiangsu, China. Electronic address: xinxinyueer717@sohu.com.
Nuo
N
Lan
Department of Pharmacy, Xuzhou Medical College, Jiangsu, China. Electronic address: boshitom@126.com.
Xiaoxing
X
Yin
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002956:Citrulline / Q000494:pharmacology / Q000627:therapeutic use*; D000431:Ethanol; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D020011:Protective Agents; D016753:Interleukin-10; D002956:Citrulline; D009569:Nitric Oxide; D000431:Ethanol; D008315:Malondialdehyde; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione