Investigating primary cilia in cultured metanephric mesenchymal cells.
Primary cilia are present in most vertebrate cells. They have complex structures that are required for signal transduction in developing tissues. The embryonic kidney consists of two major cell lineages, ureteric and metanephric mesenchyme. Here, we describe a method to isolate metanephric mesenchyme from ureteric bud, culture metanephric mesenchyme cells, and study primary cilia in cell culture.
Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, University of Toronto, Toronto, ON, Canada.
Lijun
L
Chi
Norman
N
Rosenblum
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002923:Cilia / Q000648:ultrastructure*; D007668:Kidney / Q000166:cytology / Q000196:embryology*; D008648:Mesoderm / Q000166:cytology*; D051379:Mice