Allonocardiopsis opalescens gen. nov., sp. nov., a new member of the suborder Streptosporangineae, from the surface-sterilized fruit of a medicinal plant.
An endophytic actinomycete, designated strain I10A-01259(T), was isolated from a surface-sterilized fruit of Lonicera maackii (Rupr.) Maxim., a medicinal plant, which was collected from a suburb of Beijing, China. Whole-cell hydrolysates of the isolate contained galactose and meso-diaminopimelic acid. The predominant phospholipids were phosphatidylglycerol and diphosphatidylglycerol; the menaquinones consisted mainly of MK-9, MK-11 and MK-12, with a minor amount of MK-10. The major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. Comparative analysis of 16S rRNA gene sequences indicated that strain I10A-01259(T) was most closely related to Nocardiopsis arabia S186(T) (93.2 % sequence similarity), Thermobifida halotolerans YIM 90462(T) (93.0 %) and other strains of genera within the families Nocardiopsaceae and Thermomonosporaceae. On the phylogenetic tree based on 16S rRNA gene sequences, strain I10A-01259(T) fell within the radius of the suborder Streptosporangineae, in which the strain formed a distinct lineage next to the genera of the families Nocardiopsaceae and Thermomonosporaceae. Based on the data from our polyphasic taxonomic study, a novel genus and species, Allonocardiopsis opalescens gen. nov., sp. nov., are proposed within the suborder Streptosporangineae. The type strain of Allonocardiopsis opalescens is strain I10A-01259(T) ( = CPCC 203428(T)  = DSM 45601(T)  = KCTC 19844(T)).
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Hui-Jing
HJ
Du
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Yu-Qin
YQ
Zhang
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Hong-Yu
HY
Liu
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Jing
J
Su
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Yu-Zhen
YZ
Wei
Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing 100102, PR China.
Bai-Ping
BP
Ma
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China.
Bao-Lin
BL
Guo
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Li-Yan
LY
Yu
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005638:Fruit / Q000382:microbiology*; D031239:Lonicera / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010946:Plants, Medicinal / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2