Biotechnological utilization of biodiesel-derived glycerol for the production of ribonucleotides and microbial biomass.
Ten yeast strains were evaluated concerning their capabilities to assimilate biodiesel-derived glycerol in batch cultivation. The influence of glycerol concentration, temperature, pH and yeast extract concentration on biomass production was studied for the yeast selected. Further, the effect of agitation on glycerol utilization by the yeast Hansenula anomala was also studied. The yeast H. anomala CCT 2648 showed the highest biomass yield (0.30 g g(-1)) and productivity (0.19 g L(-1) h(-1)). Citric acid, succinic acid, acetic acid and ethanol were found as the main metabolites produced. The increase of yeast extract concentration from 1 to 3 g L(-1) resulted in high biomass production. The highest biomass concentration (21 g L(-1)), yield (0.45 g g(-1)) and productivity (0.31 g L(-1) h(-1)), as well as ribonucleotide production (13.13 mg g(-1)), were observed at 700 rpm and 0.5 vvm. These results demonstrated that glycerol from biodiesel production process showed to be a feasible substrate for producing biomass and ribonucleotides by yeast species.
Departamento de Biotecnologia-LOT, Escola de Engenharia de Lorena, Universidade de São Paulo, Estrada Municipal do Campinho, PO Box 116, 12602810 Lorena, São Paulo, Brazil.
Juan Daniel
JD
Rivaldi
Boutros Fouad
BF
Sarrouh
Ricardo de Freitas
Rde F
Branco
Ismael Maciel
IM
de Mancilha
Silvio Silvério
SS
da Silva
D000332:Aerobiosis; D000693:Anaerobiosis; D061249:Batch Cell Culture Techniques; D056804:Biofuels / Q000032:analysis*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002264:Carboxylic Acids / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D012313:RNA / Q000096:biosynthesis; D012265:Ribonucleotides / Q000096:biosynthesis*; D015003:Yeasts / Q000378:metabolism*
D056804:Biofuels; D002264:Carboxylic Acids; D012265:Ribonucleotides; D000431:Ethanol; D012313:RNA; D005990:Glycerol