Hybridizing ability and nuclease resistance profile of backbone modified cationic phosphorothioate oligonucleotides.
Various stereochemically pure cationic phosphorothioate oligonucleotides bearing aminoalkyl moieties were synthesized, and their duplex-forming ability against single-stranded DNA (ssDNA), single-stranded RNA (ssRNA) and triplex-forming ability against double-stranded DNA (dsDNA) were evaluated by UV melting experiments. The cationic Rp stereoisomers showed improved duplex-forming ability against ssDNA, triplex-forming ability against dsDNA and nuclease stability.
Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh. rahman_du@yahoo.com
S M Abdur
SM
Rahman
Takeshi
T
Baba
Tetsuya
T
Kodama
Md Ariful
MA
Islam
Satoshi
S
Obika
D002412:Cations / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism*; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D054735:Phosphorothioate Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000378:metabolism; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D002412:Cations; D004277:DNA, Single-Stranded; D054735:Phosphorothioate Oligonucleotides; D012313:RNA; D004720:Endonucleases