Comparison of acute oxidative stress on rat lung induced by nano and fine-scale, soluble and insoluble metal oxide particles: NiO and TiO2.
The aim of the present study is to understand the association between metal ion release from nickel oxide (NiO) nanoparticles and induction of oxidative stress in the lung. NiO nanoparticles have cytotoxic activity through nickel ion release and subsequent oxidative stress. However, the interaction of oxidative stress and nickel ion release in vivo is still unclear. In the present study, we examined the effect of metal ion release on oxidative stress induced by NiO nanoparticles. Additionally, nano and fine TiO(2) particles as insoluble particles were also examined. Rat lung was exposed to NiO and TiO(2) nanoparticles by intratracheal instillation. The NiO nanoparticles released Ni(2+) in dispersion. Bronchoalveolar lavage fluid (BALF) was collected at 1, 24, 72 h and 1 week after instillation. The lactate dehydrogenase (LDH) and HO-1 levels were elevated at 24 and 72 h after instillation in the animals exposed to the NiO nanoparticles. On the other hand, total hydroxyoctadecadienoic acid (tHODE), which is an oxidative product of linoleic acid, as well as SP-D and α-tochopherol levels were increased at 72 h and 1 week after instillation. Fine NiO particles, and nano and fine TiO(2) particles did not show lung injury or oxidative stress from 1 h to 1 week after instillation. These results suggest that Ni(2+) release is involved in the induction of oxidative stress by NiO nanoparticles in the lung. Ni(2+) release from NiO nanoparticles is an important factor inoxidative stress-related toxicity, not only in vitro but also in vivo.
Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan. masa-horie@med.uoeh-u.ac.jp
Masanori
M
Horie
Hiroko
H
Fukui
Shigehisa
S
Endoh
Junko
J
Maru
Arisa
A
Miyauchi
Mototada
M
Shichiri
Katsuhide
K
Fujita
Etsuo
E
Niki
Yoshihisa
Y
Hagihara
Yasukazu
Y
Yoshida
Yasuo
Y
Morimoto
Hitoshi
H
Iwahashi
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000187:drug effects; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D053768:Metal Nanoparticles / Q000633:toxicity*; D009532:Nickel / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D037663:Pulmonary Surfactant-Associated Protein D / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D014025:Titanium / Q000633:toxicity*; D024502:alpha-Tocopherol / Q000378:metabolism
D011506:Proteins; D037663:Pulmonary Surfactant-Associated Protein D; C009495:titanium dioxide; D009532:Nickel; C028007:nickel monoxide; D014025:Titanium; D007770:L-Lactate Dehydrogenase; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D024502:alpha-Tocopherol