Identification of novel glutathione transferases in Echinococcus granulosus. An evolutionary perspective.
Glutathione transferase enzymes (GSTs) constitute a major detoxification system in helminth parasites and have been related to the modulation of host immune response mechanisms. At least three different GSTs classes have been described in Platyhelminthes: Mu, Sigma and Omega. Mining the genome of Echinococcus multilocularis and the ESTs databases of Taenia solium and E. granulosus identified two new GSTs from the cestode E. granulosus, named EgGST2 and EgGST3. It also revealed that the Omega class of GSTs was absent from the Taenidae family. EgGST2 and EgGST3 are actively expressed in the parasite. In order to know the origin of these new GSTs, in silico analyses were performed. While EgGST2 is classified as belonging to the Sigma class, the data obtained for EgGST3 allowed a less clear interpretation. The study of the evolutionary relatedness based on the C-terminal domain sequence, gene structure conservation and three-dimensional structure predictions, suggests that EgGST3 is derived from the Platyhelminthes' Sigma-class cluster. Interestingly, the N-terminal domain displays some characteristic Omega-class residues, including a Cys residue that is likely to be involved in the catalytic mechanism. We discuss different evolutionary scenarios that could explain the observed patterns.
Laboratorio de Organización y Evolución del Genoma, Facultad de Ciencias (UDELAR), Iguá 4225, CP 11400 Montevideo, Uruguay.
Andrés
A
Iriarte
Paula
P
Arbildi
Silvana
S
La-Rocca
Héctor
H
Musto
Verónica
V
Fernández
D000595:Amino Acid Sequence; D000818:Animals; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D048209:Echinococcus granulosus / Q000201:enzymology* / Q000235:genetics*; D048210:Echinococcus multilocularis / Q000201:enzymology / Q000235:genetics; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D041201:Taenia solium / Q000201:enzymology / Q000235:genetics
D018509:DNA, Helminth; D005982:Glutathione Transferase