Immunosuppression effects of bone marrow mesenchymal stem cells on renal interstitial injury in rats with unilateral ureteral obstruction.
We investigated the effects of intravenously administered bone marrow mesenchymal stem cells (BMSCs) on renal interstitial inflammation and fibrosis. In unilateral ureteral obstruction (UUO) rats, the CD4(+)CD25(+) regulatory T-cell (Treg) cell, macrophage population and some inflammation related cytokines were tested. In the BMSCs -treated rats, renal exhibited lower renal Masson scores, decreased macrophage infiltration and interferon gamma (IFNγ) expression, and increased forkhead transcription factor (Foxp3) and interleukin-10 (IL-10) expression. No significant differences in the CD4(+)CD25(+) Treg population and renal transforming growth factor-β1 (TGFβ1) expression were observed between BMSCs-treated group and control group (p>0.05). In conclusion, BMSCs infusion leads to an anti-inflammation response in the early stage of UUO which may related to paracine mechanism.
The Department of Nephrology, Shanghai Jiao Tong University School of Medicine Affiliated Ninth People's Hospital, Shanghai 200011, China.
Ying-Li
YL
Liu
Ying-Deng
YD
Wang
Feng
F
Zhuang
Shu-Li
SL
Xian
Jun-Yan
JY
Fang
Wei
W
Su
Wei
W
Zhang
D000818:Animals; D001853:Bone Marrow / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D053331:Ectodysplasins / Q000276:immunology; D051858:Forkhead Transcription Factors / Q000276:immunology; D007668:Kidney / Q000276:immunology* / Q000293:injuries; D008297:Male; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D014517:Ureteral Obstruction / Q000276:immunology*
D053331:Ectodysplasins; D051858:Forkhead Transcription Factors; C500522:Foxp3 protein, rat