Isolation and functional analysis of Thmfs1, the first major facilitator superfamily transporter from the biocontrol fungus Trichoderma harzianum.
A novel major facilitator superfamily (MFS) transporter gene, Thmfs1, was isolated from Trichoderma harzianum (T. harzianum). A Thmfs1 over-expressing mutant displayed enhanced antifungal activity and fungicide tolerance, while the Thmfs1 disruption mutant showed the opposite trend. Trichodermin production in Thmfs1 disruption group (185 mg l(-1)) was decreased by less than 17 % compared to the parental strain, suggesting that Thmfs1 is not mainly responsible for trichodermin secretion. Real-time PCR showed that Thmfs1 transcript level could be induced by a certain range of trichodermin concentrations, while expression of Tri5, encoding a trichodiene synthase, was strongly inhibited under these conditions. To our knowledge, Thmfs1 is the first MFS transporter gene identified in T. harzianum.
Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, 258 Xueyuan Street, Xiasha, Hangzhou, 310018 Zhejiang, China. lovemotherchina@163.com
Mu
M
Liu
Jun
J
Liu
Wei Min
WM
Wang
D016960:Agrobacterium tumefaciens / Q000235:genetics; D025141:Drug Resistance, Fungal; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D009154:Mutation; D010572:Pest Control, Biological; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014242:Trichoderma / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014243:Trichodermin / Q000302:isolation & purification / Q000378:metabolism*
D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; D014243:Trichodermin