Derivation of induced pluripotent stem cells from fetal human skin fibroblasts.
The isolation and study of autologous human stem cells remain among the most urgent problems in cell biology and biomedicine to date. Induced pluripotent stem cells can be derived from human somatic cells by the overexpression of a number of genes. In this study we reprogrammed fetal human skin fibroblasts by transduction with retroviral vectors carrying murine  Oct4  ,  Sox2  ,  Klf4  , and  c-Myc  cDNAs. As a result, cells with the protein expression and gene transcription pattern characteristic of human embryonic stem cells were derived. These induced pluripotent cells are capable of differentiation  in vitro  into the ectoderm, mesoderm, and endoderm derivatives.
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences.
S P
SP
Medvedev
A A
AA
Malakhova
E V
EV
Grigor'eva
A I
AI
Shevchenko
E V
EV
Dementyeva
I A
IA
Sobolev
I N
IN
Lebedev
A G
AG
Shilov
I F
IF
Zhimulev
S M
SM
Zakian