Microarray-based gene  expression profiles in rabbit retina due to negative pressure suction.
We investigated a possible molecular pathogenesis  involving retinal ganglion cell apoptosis following transient high intraocular  pressure. Changes in the gene expression profiles of the retina were detected  via gene chip methodology. Twelve New Zealand white rabbits were randomly  assigned to control and 3-min negative pressure suction groups. The control  group was treated only with a laser, and the experimental group was also  treated with suction for 3 min, using a negative pressure generator. Total RNA  was then extracted from the retinal tissue at different recovery stages to  analyze gene expression profiles using the Agilent rabbit one-way gene chip.  The groups were then compared. Immediately after negative pressure suction  induction, 704 genes were differentially expressed. Among these, 485 genes were  upregulated, and 219 were downregulated. Expression of the genes encoding  CRYAA, CRYAB, and TLR3 genes, which are involved in apoptosis, was elevated. The  KRT18 gene, which is involved in apoptosis, had reduced expression. Seven days  after negative pressure suction, 482 genes were differentially expressed. Among  these, 178 genes were upregulated, and 304 were downregulated. Expression of  the genes encoding CRYAB, IL1-BETA and IL1R1, which are involved in apoptosis,  was upregulated. Ten days after negative pressure suction, 402 genes were  differentially expressed. Of these, 213 genes were upregulated, and 189 were  downregulated. Apoptosis genes CRYAB, CRYBA3, CRYBB2, IL1- BETA, and IL1R1  showed higher expression levels. We concluded that negative pressure suction  for long periods of time (for example, 3 min) results in changes in gene  expression. Genes with higher fold changes help protect retinal ganglion cells  from apoptosis. We suggest that promoting the expression of these genes should  be considered as a new means for treating ischemic-hypoxic retinopathy.
Department of Ophthalmology, Affiliated Hospital, Inner Mongolia Medical College, Hohhot, Inner Mongolia, China. zhaohaixia@sohu.com
H X
HX
Zhao
C M
CM
Niu
W Y
WY
Guan
D000818:Animals; D016000:Cluster Analysis; D004587:Electrophoresis, Agar Gel; D005456:Fluorescent Dyes / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020411:Oligonucleotide Array Sequence Analysis; D011312:Pressure; D012313:RNA / Q000302:isolation & purification; D011817:Rabbits; D012160:Retina / Q000378:metabolism*; D013194:Staining and Labeling; D013396:Suction
D005456:Fluorescent Dyes; D012313:RNA