An E. coli SOS-EGFP biosensor for fast and sensitive detection of DNA damaging agents.
An E. coli SOS-EGFP biosensor which expresses enhanced green fluorescent protein as a reporter protein under the control of recA gene promoter in SOS response was constructed for detection of DNA damage and evaluation of DNA damaging chemicals. The chemicals that may cause substantial DNA damage will trigger SOS response in the constructed bacterial biosensor, and then the reporter egfp gene under the control of recA promoter is stimulated to express as a fluorescent protein, allowing fast and sensitive fluorescence detection. Interestingly, this biosensor can be simultaneously applied for evaluation of genotoxicity and cytotoxicity. The SOS-EGFP bacterial biosensor provides a sensitive, specific and simple method for detecting known and potential DNA damaging chemicals.
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Zhilan
Z
Chen
Meiling
M
Lu
Dandan
D
Zou
Hailin
H
Wang
D015374:Biosensing Techniques; D004249:DNA Damage / Q000187:drug effects* / Q000502:physiology; D004269:DNA, Bacterial / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000633:toxicity*; D011401:Promoter Regions, Genetic; D013014:SOS Response, Genetics / Q000187:drug effects*
D004269:DNA, Bacterial; D009153:Mutagens; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins