Quantification of the affinities and kinetics of protein interactions using silicon nanowire biosensors.
Monitoring the binding affinities and kinetics of protein interactions is important in clinical diagnostics and drug development because such information is used to identify new therapeutic candidates. Surface plasmon resonance is at present the standard method used for such analysis, but this is limited by low sensitivity and low-throughput analysis. Here, we show that silicon nanowire field-effect transistors can be used as biosensors to measure protein-ligand binding affinities and kinetics with sensitivities down to femtomolar concentrations. Based on this sensing mechanism, we develop an analytical model to calibrate the sensor response and quantify the molecular binding affinities of two representative protein-ligand binding pairs. The rate constant of the association and dissociation of the protein-ligand pair is determined by monitoring the reaction kinetics, demonstrating that silicon nanowire field-effect transistors can be readily used as high-throughput biosensors to quantify protein interactions.
Department of Electrical Engineering, Yale University, New Haven, Connecticut                 06520, USA.
Xuexin
X
Duan
Yue
Y
Li
Nitin K
NK
Rajan
David A
DA
Routenberg
Yorgo
Y
Modis
Mark A
MA
Reed
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D024243:HMGB1 Protein / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D053770:Nanowires / Q000737:chemistry*; D011485:Protein Binding; D012825:Silicon / Q000737:chemistry*
D024243:HMGB1 Protein; D004247:DNA; D012825:Silicon