Imaging microglial activation and glucose consumption in a mouse model of Alzheimer's disease.
In Alzheimer's disease (AD), persistent microglial activation as sign of chronic neuroinflammation contributes to disease progression. Our study aimed to in vivo visualize and quantify microglial activation in 13- to 15-month-old AD mice using [(11)C]-(R)-PK11195 and positron emission tomography (PET). We attempted to modulate neuroinflammation by subjecting the animals to an anti-inflammatory treatment with pioglitazone (5-weeks' treatment, 5-week wash-out period). [(11)C]-(R)-PK11195 distribution volume values in AD mice were significantly higher compared with control mice after the wash-out period at 15 months, which was supported by immunohistochemistry data. However, [(11)C]-(R)-PK11195 μPET could not demonstrate genotype- or treatment-dependent differences in the 13- to 14-month-old animals, suggesting that microglial activation in AD mice at this age and disease stage is too mild to be detected by this imaging method.
European Institute for Molecular Imaging, Westfalian Wilhelms-University Münster, Münster, Germany.
Sara
S
Rapic
Heiko
H
Backes
Thomas
T
Viel
Markus P
MP
Kummer
Parisa
P
Monfared
Bernd
B
Neumaier
Stefan
S
Vollmar
Mathias
M
Hoehn
Annemie
A
Van der Linden
Michael T
MT
Heneka
Andreas H
AH
Jacobs
D000544:Alzheimer Disease / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000378:metabolism; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000473:pathology; D001931:Brain Mapping; D039481:CD11b Antigen / Q000378:metabolism; D002250:Carbon Radioisotopes; D004195:Disease Models, Animal; D019788:Fluorodeoxyglucose F18; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007546:Isoquinolines; D051379:Mice; D008822:Mice, Transgenic; D017628:Microglia / Q000000981:diagnostic imaging / Q000473:pathology*; D009154:Mutation / Q000235:genetics; D049268:Positron-Emission Tomography; D053764:Presenilin-1 / Q000235:genetics
D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D039481:CD11b Antigen; D002250:Carbon Radioisotopes; D007546:Isoquinolines; C508853:PSEN1 protein, human; D053764:Presenilin-1; D019788:Fluorodeoxyglucose F18; D005947:Glucose; C037850:PK 11195