Cardiac-specific inducible and conditional gene targeting in mice.
Mouse genetic engineering has revolutionized our understanding of the molecular and genetic basis of heart development and disease. This technology involves conditional tissue-specific and temporal transgenic and gene targeting approaches, as well as introduction of polymorphisms into the mouse genome. These approaches are increasingly used to elucidate the genetic pathways underlying tissue homeostasis, physiology, and pathophysiology of adult heart. They have also led to the development of clinically relevant models of human cardiac diseases. Here, we review the technologies and their limitations in general and the cardiovascular research community in particular.
BIO5 Institute and Department of Cellular & Molecular Medicine, University of Arizona, Tucson, AZ, USA.
Thomas
T
Doetschman
Mohamad
M
Azhar
D017398:Alternative Splicing; D000818:Animals; D003851:Deoxyribonucleases / Q000378:metabolism; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D006321:Heart / Q000196:embryology / Q000503:physiopathology; D006329:Heart Conduction System / Q000378:metabolism / Q000473:pathology; D006331:Heart Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D009432:Neural Crest / Q000378:metabolism / Q000473:pathology; D010496:Pericardium / Q000378:metabolism / Q000473:pathology; D020641:Polymorphism, Single Nucleotide
D035683:MicroRNAs; D003851:Deoxyribonucleases