Fluorescence study on aggregated lysozyme and lipid bilayer interactions.
Fluorescent probes 1,6-diphenyl-1,3,5-hexatriene (DPH), pyrene, 4-dimethylaminochalcone (DMC) and 4-p-(dimethylaminostyryl)-1-dodecylpyridinium (DSP-12) have been utilized to monitor the impact of lysozyme (Lz) oligomers on physicochemical properties of phosphatidylcholine/cardiolipin (PC/CL) membranes. Analysis of spectral responses of the employed probes revealed the reduction of membrane free volume and dehydration of lipid bilayer surface upon incorporation of Lz self-assemblies. Hydrophobic interactions were found to control the binding of Lz oligomers to the lipid bilayer. Comparison of the effects of Lz monomers, oligomers and fibrils showed that soluble oligomeric intermediates exert the most destructive influence on membrane properties.
Department of Biological and Medical Physics, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov 61022, Ukraine. valtrusova@yahoo.com
Valeriya M
VM
Trusova
Galyna P
GP
Gorbenko
D000818:Animals; D002645:Chickens; D002784:Cholesterol / Q000737:chemistry; D005453:Fluorescence; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000737:chemistry
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D002784:Cholesterol; D009113:Muramidase