Sulfenylated proteins in the Medicago truncatula-Sinorhizobium meliloti symbiosis.
Reactive oxygen species such as hydrogen peroxide (H(2)O(2)), play a crucial role as signaling molecules in the establishment and functioning of the nitrogen-fixing legume-Rhizobium symbiosis. The regulation of protein function through oxidative modification has emerged as an important molecular mechanism modulating various biological processes. Protein cysteine residues are known to be sensitive targets of H(2)O(2), in a posttranslational modification called sulfenylation. We trapped and identified sulfenylated proteins in the Medicago truncatula-Sinorhizobium meliloti symbiosis, by combining the use of chemical and genetic probes with mass spectrometry analysis. We identified 44 M. truncatula proteins sulfenylated in inoculated roots (two days post infection, 2dpi) and 65 such proteins in the functioning symbiotic organ, the nodule (four weeks post infection, 4wpi); 18 proteins were identified at both time points. However, the largest functional groups at 2dpi and 4wpi were different: redox state-linked proteins early in the interaction and proteins involved in amino-acid and carbohydrate metabolism in the nodule. Twenty proteins from S. meliloti, including some directly involved in nitrogen fixation, were also identified as sulfenylated. These results suggest that sulfenylation may regulate the activity of proteins playing major roles in the development and functioning of the symbiotic interaction.
Université de Nice-Sophia Antipolis, Institut Sophia Agrobiotech, 06903 Sophia Antipolis, France.
Elodie
E
Oger
Daniel
D
Marino
Jean-Marie
JM
Guigonis
Nicolas
N
Pauly
Alain
A
Puppo
D001426:Bacterial Proteins / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D046913:Medicago truncatula / Q000235:genetics / Q000378:metabolism; D009586:Nitrogen Fixation / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000378:metabolism; D013434:Sulfenic Acids / Q000378:metabolism*; D013559:Symbiosis / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; D010940:Plant Proteins; D013434:Sulfenic Acids; D006861:Hydrogen Peroxide