Leptin regulates proliferation and apoptosis in human prostate.
This paper aimed to evaluate the leptin role on the cellular proliferation and the expression of fibroblast growth factor 2, aromatase enzyme, and apoptotic genes in the human prostate tissue.


METHODS
Fifteen samples of hyperplasic prostate tissue were divided in four symmetric parts maintained in RPMI medium supplemented with 10% fetal bovine serum, 1 ng/mL of gentamicin, and added with 50 ng/mL leptin (L) or not (C). After 3 hours of incubation, gene expression was evaluated by real time RT-PCR. Cellular proliferation was evaluated by immunohistochemistry for PCNA.


RESULTS
The leptin treatment led to an increase cellular proliferation (C = 21.8 ± 0.5; L = 64.8 ± 0.9; P < 0.0001) and in the expression of Bax (C = 0.4 ± 0.1; L = 0.9 ± 0.2; P < 0.05) while Bcl-2 (C = 19.9 ± 5.6; L = 5.6 ± 1.8; P < 0.05), Bcl-x (C = 0.2 ± 0.06; L = 0.07 ± 0.02; P < 0.05), and aromatase expressions (C = 1.9 ± 0.6; L = 0.4 ± 0.1; P < 0.04) were significantly reduced.


CONCLUSIONS
Leptin has an important role in maintaining the physiological growth of the prostate since it stimulates both cellular proliferation and apoptosis, with the decrement in the aromatase gene expression.
Urogenital Research Unit, State University of Rio de Janeiro, 20551-030 Rio de Janeiro, Brazil.
Eduardo
E
Leze
Jorge Luiz
JL
Alves-Pereira
Sicilia
S
Colli
Fernanda Silveira
FS
Cavalcante
Francisco
F
José Sampaio
Cristiane
C
da Fonte Ramos
D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D020738:Leptin / Q000494:pharmacology*; D008297:Male; D011467:Prostate / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism
D020738:Leptin