Cortisol treatment reduces ghrelin signaling and food intake in tilapia, Oreochromis mossambicus.
It is well known that after a stressor, levels of plasma cortisol rise, inducing physiological changes within the animal that are directed toward maintaining homeostasis. Less well understood is the role of cortisol in regulating food intake in teleosts. This study investigated the effect of cortisol on food intake and regulation of the neuroendocrine appetite-stimulating hormones, neuropeptide Y (NPY) and ghrelin, in tilapia (Oreochromis mossambicus). Male and female tilapia were randomly assigned to one of the following treatments: unhandled control, vehicle-injected control, or cortisol (2 μg/g BW). Food intake was determined 24 h after injection during a 1-h feeding trial. Cortisol reduced food intake (P<0.001). An identical study was conducted to measure the effects of 24-h cortisol treatment on the endocrine regulators of food intake. Cortisol reduced stomach expression of ghrelin mRNA (P<0.05) and plasma concentrations of ghrelin (P<0.05). In the hypothalamus/optic tectum cortisol reduced levels of GHSR1a-LR (biologically active ghrelin receptor) mRNA. In the telencephalon/preoptic area cortisol significantly reduced levels of NPY and GHSR1b-LR (biologically inactive ghrelin receptor) mRNA. These findings suggest that anorexigenic actions of cortisol may be mediated via two separate pathways: (1) reducing circulating ghrelin levels as well as GHSR1a-LR expression in the hypothalamus/optic tectum and/or (2) suppressing NPY expression in the telencephalon/preoptic area.
Department of Biology, California State University at Fresno, Fresno, CA 93740, USA.
W J
WJ
Janzen
C A
CA
Duncan
L G
LG
Riley
D000818:Animals; D001786:Blood Glucose; D004435:Eating / Q000187:drug effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D054439:Ghrelin / Q000378:metabolism*; D006854:Hydrocortisone / Q000494:pharmacology*; D008297:Male; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054440:Receptors, Ghrelin / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D017210:Tilapia / Q000502:physiology*
D001786:Blood Glucose; D054439:Ghrelin; D009478:Neuropeptide Y; D012333:RNA, Messenger; D054440:Receptors, Ghrelin; D006854:Hydrocortisone