Development of microsatellite markers for the small  yellow croaker Larimichthys polyactis (Sciaenidae) by cross-species  amplification.
The small yellow croaker (Larimichthys polyactis)  is a highly valued fish for human consumption found in the Western Pacific that  was considered endangered until recently because of overfishing. We selected  microsatellite markers for this species from markers developed for Miichthys  miiuy, also of the family Sciaenidae. Among 43 markers polymorphic for M.  miiuy, 11 were found to be polymorphic for L. polyactis.  Characterization of these 11 loci was made based on 30 L. polyactis individuals  collected by trawling in the Zhoushan Fishing Ground, Zhejiang Province, China.  Total genomic DNA was isolated from fin clips. The number of alleles per locus  ranged from 4 to 10, with a mean of 5.82, while the effective number of alleles  ranged from 1.64 to 10.00, with a mean of 3.22. Observed and expected  heterozygosities ranged from 0.17 to 0.72 and from 0.39 to 0.81, respectively.  Significant deviation from Hardy-Weinberg equilibrium was found at four loci,  after applying Bonferroni's correction. There was no significant association  between any of the pairs of microsatellite loci, hence allelic variation at  these loci was considered independent. These 11 polymorphic microsatellite loci  will be useful for genetic diversity analysis and molecular-assisted breeding  for L. polyactis.
Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, PR China.
D Q
DQ
Sun
H Y
HY
Li
T J
TJ
Xu
R X
RX
Wang
D000483:Alleles; D000818:Animals; D014644:Genetic Variation / Q000235:genetics; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D010473:Perciformes / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics