In vitro culture of embryonic kidney rudiments and isolated ureteric buds.
In vitro culture of embryonic kidney rudiments has been utilized to study a variety of cellular processes and developmental mechanisms. Here, we describe two-dimensional (2D) culture of embryonic kidney rudiments on Transwell filters and three-dimensional (3D) cultures in collagen gels in detail, and 3D culture of isolated ureteric bud (UB) in Matrigel with BSN-conditioned media.
Pediatrics Department, University of California, San Diego, CA, USA.
Xing
X
Zhang
Kevin T
KT
Bush
Sanjay K
SK
Nigam
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D004867:Equipment Design; D005109:Extracellular Matrix / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D007668:Kidney / Q000166:cytology / Q000196:embryology / Q000254:growth & development*; D051379:Mice; D009924:Organ Culture Techniques / Q000295:instrumentation / Q000379:methods*; D051381:Rats
D003470:Culture Media; D005782:Gels