Development of a biosensor for human blood: new routes to body fluid identification.
The identification of human blood at a crime scene can provide crucial information to an investigation whilst also providing a source of nuclear material which can be targeted for DNA profiling. Here, we report on the development of an immunofluorescent biosensor for the identification of human blood which has the potential to overcome the drawbacks of the current body fluid identification techniques. An antibody (Ab) raised against human erythrocytes was conjugated to fluorescent semiconductor quantum dots (QDs) by sulfhydryl chemistry. The conjugation was verified by agarose gel electrophoresis and immunohistochemistry. Incubation of liquid blood samples with the conjugated nanocrystals was shown to quench the fluorescence emission spectra in a concentration-dependent manner. A different effect was observed with unconjugated QDs incubated in blood. Full profiles were obtained from blood samples previously treated with the Ab-QDs, demonstrating that the method does not interfere with DNA profiling. To our knowledge, this is the first example of a hybrid Ab-QD sensor that has the potential to be employed for the identification of human blood. The results of this study are expected to open up a new research direction in the field of body fluid detection.
School of Biomedical and Health Sciences, Department of Forensic Science and Drug Monitoring, King's College London, London, UK. nunzianda.frascione@kcl.ac.uk
Nunzianda
N
Frascione
Vania
V
Pinto
Barbara
B
Daniel
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods*; D001826:Body Fluids / Q000737:chemistry*; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D045663:Quantum Dots