Immunolocalization of bone morphogenetic protein 2 during the early healing events after guided bone regeneration.
OBJECTIVE
The objective of this study was to evaluate the immunolocalization of bone morphogenetic protein 2 (BMP-2) after autogenous block grafting covered or not with an e-PTFE membrane.


METHODS
Forty-eight rats were divided into 2 groups, autogenous block graft (B) and autogenous block graft + e-PTFE membrane (MB), and were evaluated by immunohistochemistry at baseline and 3, 7, 14, 21, and 45 days.


RESULTS
The largest number of positive cells in the recipient bed was observed after 3 days in both groups. At the graft border, the largest number of positive cells was seen after 7 days in group B and after 14 days in group MB. The highest proportion of staining in the graft was observed after 3 days in group B and after 21 days in group MB.


CONCLUSIONS
High proportions of stain were related to intense revascularization and osteogenesis. Except for the interface, BMP-2 staining occurred later in group MB than in group B in all structures analyzed.
Department of Stomatology, University of São Paulo, São Paulo, Brazil. andreacdemarco@gmail.com
Andréa Carvalho
AC
De Marco
Maria Aparecida Neves
MA
Jardini
Filipe
F
Modolo
Fábio Daumas
FD
Nunes
Luiz Antonio Pugliesi Alves
LA
de Lima
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis* / Q000502:physiology; D001861:Bone Regeneration; D016025:Bone Transplantation; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D008297:Male; D008334:Mandible / Q000098:blood supply / Q000601:surgery; D008567:Membranes, Artificial; D018919:Neovascularization, Physiologic; D010006:Osteoblasts / Q000378:metabolism; D010011:Osteocytes / Q000378:metabolism; D011138:Polytetrafluoroethylene; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000378:metabolism
D055396:Bone Morphogenetic Protein 2; D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene