A cell-penetrating ratiometric nanoprobe for intracellular chloride.
NanoChlor, a nanoparticle-based fluorescent probe for chloride that is both ratiometric and capable of spontaneously penetrating neuronal cells at submillimolar concentrations, was designed and studied. NanoChlor is built on silica nanoparticles grafted with 6-methoxyquinolinium as the chloride-sensitive component and fluorescein as the reference dye. A Stern-Volmer constant of 50 M(-1) was measured in Ringer's buffer at pH 7.2, and the response to chemically induced chloride currents was recorded in real time in hippocampal cells.
Dipartimento di Scienze Chimiche, Università di Padova, via Marzolo 1, 35131 Padova, Italy.
Luca
L
Baù
Francesco
F
Selvestrel
Maria
M
Arduini
Ilaria
I
Zamparo
Claudia
C
Lodovichi
Fabrizio
F
Mancin
D000818:Animals; D002460:Cell Line; D002712:Chlorides / Q000737:chemistry*; D042541:Intracellular Space / Q000737:chemistry* / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011806:Quinolinium Compounds / Q000737:chemistry* / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry*
D002712:Chlorides; D011806:Quinolinium Compounds; D012822:Silicon Dioxide