Antioxidant action of mangrove polyphenols against gastric damage induced by absolute ethanol and ischemia-reperfusion in the rat.
Rhizophora mangle, the red mangrove, has long been known as a traditional medicine. Its bark has been used as astringent, antiseptic, hemostatic, with antifungic and antiulcerogenic properties. In this paper, we aimed to evaluate the antioxidant properties of a buthanolic fraction of the R. mangle bark extract (RM) against experimental gastric ulcer in rats. Unib-Wh rats received pretreatment of R. mangle after the induction of gastric injury with absolute ethanol and ischemia-reperfusion. Gastric tissues from both methods were prepared to the enzymatic assays, the levels of sulfhydril compounds (GSH), lipid peroxides (LPO), and the activities of glutathione reductase (GR), glutathione peroxidase (GPx), superoxide dismutase (SOD) and myeloperoxidase (MPO) were measured. The RM protected the gastric mucosa in both methods used, ethanol-induced gastric ulcer and ischemia-reperfusion, probably, by modulating the activities of the enzymes SOD, GPx, and GR and increasing or maintaining the levels of GSH; in addition, LPO levels were reduced. The results suggest that the RM antioxidant activity leads to tissue protection; thus one of the antiulcer mechanisms present on the pharmacological effects of R. mangle is the antioxidant property.
Departamento de Farmacologia, Faculdade de Ciências Médicas, UNICAMP, 13083-970 Campinas, SP, Brazil. defaria.felipe@gmail.com
Felipe Meira
FM
de-Faria
Ana Cristina Alves
AC
Almeida
Anderson
A
Luiz-Ferreira
Christiane
C
Takayama
Ricardo José
RJ
Dunder
Marcelo Aparecido
MA
da Silva
Marcos José
MJ
Salvador
Patrícia Verardi
PV
Abdelnur
Marcos Nogueira
MN
Eberlin
Wagner
W
Vilegas
Walber
W
Toma
Alba Regina Monteiro
AR
Souza-Brito
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000627:therapeutic use*; D002374:Catalase; D000431:Ethanol / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy*; D043982:Rhizophoraceae / Q000737:chemistry; D013276:Stomach Ulcer / Q000139:chemically induced* / Q000188:drug therapy*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D008054:Lipid Peroxides; D010936:Plant Extracts; D000431:Ethanol; D002374:Catalase; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione