Biocatalytically induced growth of gold nanoshells: using enzyme reaction for the controllable fabrication of nanomaterials.
In the present work, the enzymatically controlled growth process of gold nanoshells (GNSs) in the presence of O2/glucose/glucose oxidase (GOx) and its chloroaurate ion electron acceptor is described. The biocatalytically stimulated growth process is one of the bio-inspired synthetic procedures directed by biological molecules which occur under ambient conditions. It is found that hydrogen peroxide (H2O2) could enlarge the gold nanoparticles (GNPs) on the surface of GNSs precursor composites, of which the preadsorbed GNPs serve as nucleation sites for further gold deposition. Here, GOx is harnessed for its unparalled level of catalytic activity and substrate specificity while H2O2 is produced as a by-product during the oxidation of D-glucose to gluconic acid by GOx. Then the bio-generated H2O2 is used as the reducing agent in the catalytic deposition process of GNSs formation. During the procedure, the localized surface plasmon resonance peaks range across hundreds of nanometers from visible to near infrared region accompanying by the resultant formation of uniform and continuous core-shell nanostructures. The corresponding optical, morphological and enzyme kinetic properties are all well investigated. The novel protocol offers a new perspective for the bio-directed synthesis method in nanotechnology.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P R. China.
Xiaoyuan
X
Ma
Liangliang
L
Liu
Fangjing
F
Liu
Weiping
W
Qian
D055162:Biocatalysis; D003102:Colloids; D004798:Enzymes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D057145:Nanoshells; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance
D003102:Colloids; D004798:Enzymes; D006046:Gold; D006861:Hydrogen Peroxide