Stability Study of Crude Dextransucrase from Leuconostoc citreum NRRL B-742.
In the present work, the stability of crude dextransucrase from Leuconostoc citreum B-742 was evaluated in synthetic and in cashew apple juice culture broth. Optimum stability conditions for dextransucrase from L. citreum B-742 were different from the reported for its parental industrial strain enzyme (L. mesenteroides B-512F). Crude dextransucrase, from L. citreum B-742, produced using cashew apple juice as substrate, presented higher stability than the crude enzyme produced using synthetic culture medium, showing the same behavior previously reported for dextransucrase from L. mesenteroides B-512F. The crude enzyme presented good stability in cashew apple juice for 48 h at 25°C and pH 6.5.
Departamento de Tecnologia de Alimentos, Universidade Federal do Ceara, Av. Mister Hull, 2977, Bloco 858 - Campus do Pici, Fortaleza, CE 60356-000 Brazil.
Maria Cristiane
MC
Rabelo
Claudia M L
CM
Fontes
Sueli
S
Rodrigues