HPLC-assisted automated oligosaccharide synthesis.
A standard HPLC was adapted to polymer supported oligosaccharide synthesis. Solution-based reagents are delivered using a software-controlled solvent delivery system. The reaction progress and completion can be monitored in real time using a standard UV detector. All steps of oligosaccharide assembly including loading, glycosylation, deprotection, and cleavage can be performed using this setup.
Department of Chemistry and Biochemistry and the Center for Nanoscience, University of Missouri-St. Louis, One University Boulevard, St. Louis, Missouri 63121, USA.
N Vijaya
NV
Ganesh
Kohki
K
Fujikawa
Yih Horng
YH
Tan
Keith J
KJ
Stine
Alexei V
AV
Demchenko
D057205:Automation, Laboratory / Q000295:instrumentation* / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D015394:Molecular Structure; D009844:Oligosaccharides / Q000138:chemical synthesis*; D011108:Polymers / Q000737:chemistry; D012984:Software
D009844:Oligosaccharides; D011108:Polymers