Caffeic acid product from the highly copper-tolerant plant Elsholtzia splendens post-phytoremediation: its extraction, purification, and identification.
In the current study, caffeic acid was an important metabolite in the highly copper-tolerant plant Elsholtzia splendens. Preparation and purification of caffeic acid were performed on the dried biomass of the plants by means of sonication/ethanol extraction, followed by purification using a macroporous resin (D101 type) column and silica gel chromatography. The faint-yellow caffeic acid product was yielded with a purity of 98.46%, and it was chemically identified from spectra of Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance ((1)H NMR)/carbon nuclear magnetic resonance ((13)C NMR), and electrospray ionization mass spectrometry (ESI-MS). Caffeic acid is a possible product from the post-harvest processing of Elsholtzia splendens biomass.
Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
Yan
Y
Xing
Hong-yun
HY
Peng
Meng-xi
MX
Zhang
Xia
X
Li
Wei-wei
WW
Zeng
Xiao-e
XE
Yang
D001673:Biodegradation, Environmental; D018533:Biomass; D002109:Caffeic Acids / Q000302:isolation & purification* / Q000378:metabolism*; D002850:Chromatography, Gel; D003300:Copper / Q000378:metabolism; D019686:Lamiaceae / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D012989:Soil Pollutants / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared
D002109:Caffeic Acids; D012989:Soil Pollutants; D003300:Copper; C040048:caffeic acid