Interleukin-8 in the pathogenesis of primary central nervous system lymphoma in association with HIV infection.
The pathogenesis of acquired immunodeficiency syndrome-associated primary central nervous system lymphoma (AIDS-associated PCNSL) remains unclear. However, cell adhesion molecules have been reported to be strongly associated with PCNSL. In this study, we established Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs) from HIV-positive patients (LCL(HIV)) and normal individuals (LCL(N)). The expression of CD18 antigen by LCL(HIV) was stronger than that by LCL(N). We performed a cell adhesion assay using ISO-HAS, which is the human hemangiosarcoma cell line and expresses intercellular adhesion molecule 1 (CD54). The binding rates of LCL(HIV) and ISO-HAS without stimulation were higher than those of LCL(N). Further, we demonstrated that azidothymidine or simvastatin inhibited the binding rates of LCL(HIV) and ISO-HAS more significantly than those of LCL(N). Further, the levels of interleukin (IL)-8, a CD18 inducer, were higher in LCL(HIV) than in LCL(N). We conclude that interaction between IL-8 and CD18 may be critical to AIDS-related PCNSL.
Department of Hematology, Kinki University School of Medicine, Osaka, Japan.
Atsushi
A
Sasakawa
Chikara
C
Hirase
Terufumi
T
Yamaguchi
Yasuyoshi
Y
Morita
Jun-ichi
J
Miyatake
Itaru
I
Matsumura
Yasuhiro
Y
Maeda
D019380:Anti-HIV Agents / Q000494:pharmacology; D018821:CD18 Antigens / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D016543:Central Nervous System Neoplasms / Q000150:complications / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D006678:HIV / Q000502:physiology; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism*; D016483:Lymphoma, AIDS-Related / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D019821:Simvastatin / Q000494:pharmacology; D015215:Zidovudine / Q000494:pharmacology
D019380:Anti-HIV Agents; D018821:CD18 Antigens; D016209:Interleukin-8; D018799:Intercellular Adhesion Molecule-1; D015215:Zidovudine; D019821:Simvastatin