Polymerase incorporation of pyrene-nucleoside triphosphates.
Pyrene-deoxynucleoside triphosphates (dPTPs), varying by the positioning of the aromatic system, were synthesized. Their ability to function as substrates for the Klenow fragment of Escherichia coli DNA polymerase I and the TdT polymerase was assessed. The dPTPs are all equally well tolerated by the Klenow fragment, and lead to elongation of up to 5 extra nucleotides of a ssDNA primer in a TdT-mediated reaction. The tailing efficiency of the dPTPs compares favorably to other less drastically modified dNTPs.
Department of Chemistry & Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland. hollenstein@dcb.unibe.ch
Marcel
M
Hollenstein
Filip
F
Wojciechowski
Christian J
CJ
Leumann
D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism*; D017931:DNA Primers / Q000378:metabolism; D009711:Nucleotides / Q000737:chemistry / Q000378:metabolism*; D011721:Pyrenes / Q000737:chemistry*; D013379:Substrate Specificity
D017931:DNA Primers; D009711:Nucleotides; D011721:Pyrenes; C030984:pyrene; D004253:DNA Nucleotidylexotransferase; D004256:DNA Polymerase I