Mediators and cytokines in persistent allergic rhinitis and nonallergic rhinitis with eosinophilia syndrome.
BACKGROUND
Patients with nonallergic rhinitis with eosinophilia syndrome (NARES) show typical symptoms of persistent allergic rhinitis (PAR). The aim of the present study was to compare nasal cytokine patterns between NARES and PAR.


METHODS
Nasal secretions of 31 patients suffering from NARES, 20 patients with PAR to house dust mite and 21 healthy controls were collected using the cotton wool method and analyzed for interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1β (MIP-1β) by Bio-Plex Cytokine Assay as well as eosinophil cationic protein (ECP) and tryptase by UniCAP-FEIA.


RESULTS
NARES and PAR presented elevated levels of tryptase, while ECP was markedly increased solely in NARES compared to both the controls and PAR. Elevated levels of IL-1β, IL-17, IFN-γ, TNF-α and MCP-1 were found in NARES compared to the controls as well as PAR. MIP-1β was elevated in NARES and PAR, while IL-4, IL-6 and G-CSF showed increased levels in NARES, and IL- 5 was elevated in PAR only.


CONCLUSIONS
In patients with NARES and PAR, eosinophils and mast cells appear to be the pivotal cells of inflammation, reflected by high levels of tryptase and ECP as well as IL-5 and GM-CSF as factors for eosinophil migration and survival. The elevated levels of proinflammatory cytokines in NARES may indicate the chronic, self-perpetuating process of inflammation in NARES which seems to be more pronounced than in PAR. IL-17 might be a factor for neutrophilic infiltration or be responsible for remodeling processes in NARES.
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Munich, Germany. moritz.groeger@med.uni-muenchen.de
Moritz
M
Gröger
Christine
C
Klemens
Sebastian
S
Wendt
Sven
S
Becker
Martin
M
Canis
Miriam
M
Havel
Elisabeth
E
Pfrogner
Gerd
G
Rasp
Matthias F
MF
Kramer
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism*; D040002:Dermatophagoides pteronyssinus / Q000276:immunology; D004802:Eosinophilia / Q000150:complications* / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000276:immunology; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology; D012220:Rhinitis / Q000150:complications* / Q000276:immunology*; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology*; D013577:Syndrome; D055815:Young Adult
D016207:Cytokines; D018836:Inflammation Mediators