Behcet's disease with active uveitis: detection of serum protein biomarkers using MALDI-TOF-MS.
Behcet's Disease (BD) is a multisystem autoimmune disorder that lacks sensitive and specific diagnostic methods. The aim of this study was to identify potential biomarkers specific for BD and to establish a diagnostic model. Serum samples from patients with BD, Vogt-Koyanagi-Harada syndrome (VKH), and healthy controls (HC) were randomly divided into a training set (49 BD, 31 VKH, and 48 HC) and a testing set (13 BD, 10 VKH, and 11 HC). Proteomic mass spectra were generated by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Thirty-nine differential m/z peaks associated with BD were identified, and the m/z peaks at 1,644, 1,711, 2,023, 4,347, 6,628, and 8,559 were used to construct a model for the diagnosis of BD. This diagnostic model can distinguish BD from non-BD controls with a sensitivity of 83.67% (41/49) and a specificity of 89.87% (71/79). BD was detected in our blinded testing set with good sensitivity and specificity of 84.6 and 90.48%, respectively. The results suggested that proteomic fingerprint technology combining magnetic beads with MALDI-TOF-MS has potential for the diagnosis of BD. The biomarker classification model was suitable for preliminary identification of BD and could potentially serve as a useful tool for BD diagnosis and differential diagnosis.
Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.
Xin
X
Wang
Mei Fen
MF
Zhang
Jing
J
Xie
Zhi Li
ZL
Li
Peng
P
Wang
D000293:Adolescent; D000328:Adult; D001528:Behcet Syndrome / Q000097:blood* / Q000150:complications / Q000175:diagnosis; D015415:Biomarkers / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014605:Uveitis / Q000097:blood* / Q000175:diagnosis / Q000209:etiology; D014607:Uveomeningoencephalitic Syndrome / Q000097:blood* / Q000150:complications / Q000175:diagnosis; D055815:Young Adult
D015415:Biomarkers; D001798:Blood Proteins