A novel biosensor regulated by the rotator of F₀F₁-ATPase to detect deoxynivalenol rapidly.
A novel biosensor (immuno-rotary biosensor) was developed by conjugating deoxynivalenol (DON) monoclonal antibodies with the "rotator" ε-subunit of F(0)F(1)-ATPase within chromatophores with an ε-subunit monoclonal antibody-biotin-avidin-biotin linker to capture DON residues. The conjugation conditions were then optimized. The capture of DON was based on the antibody-antigen reaction and it is indicated by the change in ATP synthetic activity of F(0)F(1)-ATPase, which is measured via chemiluminescence using the luciferin-luciferase system with a computerized microplate luminometer analyzer. 10(-7)mg/ml of DON can be detected. The whole detection process requires only about 20min. This method has promising applications in the detection of small molecular compounds because of its rapidity, simplicity, and sensitivity.
Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
Yueliang
Y
Zhao
Peirong
P
Wang
Feng
F
Wang
Hongjie
H
Zhou
Weiming
W
Li
Jiachang
J
Yue
Yiming
Y
Ha
D000255:Adenosine Triphosphate / Q000138:chemical synthesis; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D009183:Mycotoxins / Q000032:analysis* / Q000276:immunology; D006180:Proton-Translocating ATPases / Q000032:analysis* / Q000737:chemistry*; D014255:Trichothecenes / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D009183:Mycotoxins; D014255:Trichothecenes; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases; C007262:deoxynivalenol