Antibiotic synergy interaction against multidrug-resistant Pseudomonas aeruginosa isolated from an abattoir effluent environment.
Pseudomonas aeruginosa is an opportunistic pathogen in environmental waters with a high prevalence of multidrug resistance. In this study the synergistic efficacy of synergy antibiotic combinations in multidrug-resistant P. aeruginosa strains isolated from an abattoir effluent was investigated. Water samples were processed using membrane filtration; Pseudomonas was isolated with Pseudomonas Isolation Agar and confirmed using polymerase chain reaction with specie-specific primer. Susceptibility studies and in vitro synergy interaction testing were carried out, employing agar dilution and Etest procedure, respectively. Resistance was noted for clinically relevant antipseudomonal agents tested. Finding from antibiotic synergy interaction studies revealed that cefepime, imipenem, and meropenem combined with amikacin resulted in statistically significant (P < 0.0001) in vitro antibiotics synergy interaction, indicating the possible use of this regimen in treatment of pseudomonal infections.
Department of Microbiology, Ambrose Alli University, Private Mail Bag 14, Ekpoma 310001, Edo State, Nigeria.
Etinosa O
EO
Igbinosa
Emmanuel E
EE
Odjadjare
Isoken H
IH
Igbinosa
Phillips O
PO
Orhue
May N O
MN
Omoigberale
Napoleon I
NI
Amhanre
D000003:Abattoirs; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D002470:Cell Survival / Q000187:drug effects; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects*; D004357:Drug Synergism; D007220:Industrial Waste; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification*; D014871:Water Microbiology
D000900:Anti-Bacterial Agents; D007220:Industrial Waste