Enhanced accumulation of secondary metabolites in hairy root cultures of Scutellaria lateriflora following elicitation.
Hairy root cultures of Scutellaria lateriflora were established using Agrobacterium rhizogenes A4 and produced acteoside (18.5 mg g(-1) dry wt), baicalin (14.5 mg g(-1) dry wt) and wogonoside (12 mg g(-1) dry wt). Yeast extract (50 μg ml(-1)) increased acteoside production 1.4-fold and flavone production 1.7-fold after 7 and 14 days of elicitation. Addition of Pectobacterium carotovorum lysate in the stationary phase of the hairy root culture stimulated only the accumulation of wogonin to 30 mg g(-1) dry wt. The production of wogonin in hairy roots could be associated with its role as a phytolaexin.
Department of Pharmacognosy with Medicinal Plant Garden, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.
Agata
A
Wilczańska-Barska
Aleksandra
A
Królicka
Daniel
D
Głód
Magdalena
M
Majdan
Anna
A
Kawiak
Mirosława
M
Krauze-Baranowska
D060054:Agrobacterium / Q000235:genetics*; D003470:Culture Media / Q000737:chemistry; D044950:Flavanones / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism*; D016973:Pectobacterium carotovorum / Q000737:chemistry; D010636:Phenols / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D031363:Scutellaria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors; D014170:Transformation, Genetic; D059808:Polyphenols
D003470:Culture Media; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D010636:Phenols; C038044:baicalin; C058956:acteoside; D059808:Polyphenols; C473995:wogonoside