Preparation and characterization of a VEGF-Fc fusion protein matrix for enhancing HUVEC growth.
To enhance vascularization of hydrophobic implants in vivo, a VEGF-Fc fusion protein consisting of vascular endothelial growth factor (VEGF) fused to the immunoglobulin G Fc domain was prepared as an artificial extracellular matrix (ECM). VEGF-Fc was stably immobilized on a polystyrene plate due to the hydrophobicity of the Fc domain, and significantly enhanced the adhesion of human umbilical vein endothelial cells (HUVECs). Additionally, the use of VEGF-Fc as an ECM markedly promoted the proliferation of HUVECs longer than 72 h and induced the reorganization of actin filaments into larger stress fibers within these cells. The VEGF-Fc fusion protein may be a promising artificial ECM for enhancing endothelial cell growth.
School of Chemical Engineering, Tianjin University, Tianjin 300072, China. yumeihua19861230@yahoo.cn
Meihua
M
Yu
Fengyi
F
Du
Hirohiko
H
Ise
Wei
W
Zhao
Yan
Y
Zhang
Yaoting
Y
Yu
Fanglian
F
Yao
Jun
J
Yang
Toshihiro
T
Akaike
D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000502:physiology*; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism; D011137:Polystyrenes / Q000737:chemistry; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D007141:Immunoglobulin Fc Fragments; D011137:Polystyrenes; D011993:Recombinant Fusion Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A