Cell surface-anchored fluorescent aptamer sensor enables imaging of chemical transmitter dynamics.
A fluorescent aptamer sensor was applied to the analysis of extracellular chemical transmitter dynamics. We utilized a tocopherol-labeled aptamer, which allowed the direct anchoring of the fluorescent aptamer on the cell surface while retaining its performance as a fluorescent sensor. The fast-responsive fluorescent DNA aptamer sensor, which targets adenine compounds, was anchored on the surface of brain astrocytes. Fluorescence imaging of the aptamer-anchored astrocytes enabled the real-time monitoring of release of adenine compounds as a gliotransmitter, which was synchronized with calcium wave propagation in neighboring cells.
INAMORI Frontier Research Center, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Takeshi
T
Tokunaga
Shigeyuki
S
Namiki
Katsuhiro
K
Yamada
Takahiro
T
Imaishi
Hiroshi
H
Nonaka
Kenzo
K
Hirose
Shinsuke
S
Sando
D000225:Adenine / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D001921:Brain / Q000166:cytology; D020013:Calcium Signaling; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D008958:Models, Molecular; D051381:Rats; D024505:Tocopherols / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D000225:Adenine; D024505:Tocopherols