Involvement of commensal bacteria may lead to dysregulated inflammatory and autoimmune responses in a mouse model for chronic nonsuppurative destructive cholangitis.
BACKGROUND
We previously reported a mouse model of primary biliary cirrhosis (PBC)-like chronic nonsuppurative destructive cholangitis (CNSDC), in which frequent injections of Streptococcus intermedius induced CNSDC and autoantibody production. The present study was performed to verify the model by examining 1) the reappearance of the PBC-like CNSDC after lymphocyte transfer from model to naïve mice, 2) the involvement of autophagy, and 3) the influence of the strain difference.


METHODS
Mice were inoculated with S. intermedius weekly for 8 weeks, then sacrificed to obtain samples. Spleen cells obtained from S. intermedius-inoculated mice were transferred to RAG2(-/-) mice.


RESULTS
CNSDC and elevated serum level of anti-gp210 titers were observed in S. intermedius-inoculated C57BL/6 mice, similar to the results of our previous report using BALB/c mice. Portal inflammation was induced in the livers of RAG2(-/-) mice by the transfer of spleen cells from S. intermedius-inoculated C57BL/6 mice. Among the inflammatory cells in the RAG2(-/-) mice, CD3-positive cells were predominant. Autophagosome-like structures were detected histologically, in the cytoplasm of infiltrated cells around the bile ducts in the livers of S. intermedius-inoculated both C57BL/6 and BALB/c mice. In S. intermedius-inoculated C3H/HeJ mice, inflammation in the portal area was less extensive than that in the hepatic parenchyma.


CONCLUSIONS
Bacterial component(s) and sequentially upregulated innate and acquired immune responses, accompanied by autophagy, might trigger CNSDC, via autoimmune mechanisms. Throughout the generation of bacteria-triggered PBC-like CNSDC, strain difference may influence the response to S. intermedius-inoculation in the liver.
Department of Microbiology and Immunology, Tokyo Women's Medical University, 8-1, Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan. haruta@research.twmu.ac.jp
Ikuko
I
Haruta
Ken
K
Kikuchi
Minoru
M
Nakamura
Katsuhiko
K
Hirota
Hidehito
H
Kato
Hiroshi
H
Miyakawa
Noriyuki
N
Shibata
Yoichiro
Y
Miyake
Etsuko
E
Hashimoto
Keiko
K
Shiratori
Junji
J
Yagi
D019264:Adoptive Transfer; D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D008105:Liver Cirrhosis, Biliary / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D028861:Nuclear Pore Complex Proteins / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013290:Streptococcal Infections / Q000276:immunology* / Q000473:pathology; D034367:Streptococcus intermedius
D000974:Antibodies, Antinuclear; D004268:DNA-Binding Proteins; D028861:Nuclear Pore Complex Proteins; C512902:Nup210 protein, mouse; C499308:Rag2 protein, mouse