Use of panitumumab-IRDye800 to image microscopic head and neck cancer in an orthotopic surgical model.
BACKGROUND
Fluorescence imaging hardware (SPY) has recently been developed for intraoperative assessment of blood flow via detection of probes emitting in the near-infrared (NIR) spectrum. This study sought to determine if this imaging system was capable of detecting micrometastatic head and neck squamous cell carcinoma (HNSCC) in preclinical models.


METHODS
A NIR fluorescent probe (IRDye800CW) was covalently linked to a monoclonal antibody targeting epidermal growth factor receptor (EGFR; panitumumab) or nonspecific IgG. HNSCC flank (SCC-1) and orthotopic (FADU and OSC19) xenografts were imaged 48-96 h after systemic injection of labeled panitumumab or IgG. The primary tumor and regional lymph nodes were dissected using fluorescence guidance with the SPY system and grossly assessed with a charge-coupled NIR system (Pearl). Histologic slides were also imaged with a NIR charged-coupled device (Odyssey) and fluorescence intensity was correlated with pathologic confirmation of disease.


RESULTS
Orthotopic tongue tumors were clearly delineated from normal tissue with tumor-to-background ratios of 2.9 (Pearl) and 2.3 (SPY). Disease detection was significantly improved with panitumumab-IRDye compared to IgG-IRDye800 (P < 0.05). Tissue biopsy samples (average size 3.7 mm) positive for fluorescence were confirmed for pathologic disease by histology and immunohistochemistry (n = 25 of 25). Biopsy samples of nonfluorescent tissue were proven to be negative for malignancy (n = 28 of 28). The SPY was able to detect regional lymph node metastasis (<1.0 mm) and microscopic areas of disease. Standard histological assessment in both frozen and paraffin-embedded histologic specimens was augmented using the Odyssey.


CONCLUSIONS
Panitumumab-IRDye800 may have clinical utility in detection and removal of microscopic HNSCC using existing intraoperative optical imaging hardware and may augment analysis of frozen and permanent pathology.
Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.
C Hope
CH
Heath
Nicholas L
NL
Deep
Larissa
L
Sweeny
Kurt R
KR
Zinn
Eben L
EL
Rosenthal
D000818:Animals; D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000601:surgery; D066246:ErbB Receptors / Q000037:antagonists & inhibitors / Q000276:immunology; D006258:Head and Neck Neoplasms / Q000175:diagnosis* / Q000601:surgery; D006801:Humans; D007124:Immunoenzyme Techniques; D007211:Indoles; D008207:Lymphatic Metastasis; D051379:Mice; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D008953:Models, Anatomic; D061848:Optical Imaging; D000077544:Panitumumab; D019265:Spectroscopy, Near-Infrared; D025321:Surgery, Computer-Assisted; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; C427728:IRDye800; D007211:Indoles; D000077544:Panitumumab; C512478:EGFR protein, human; D066246:ErbB Receptors