Use of human perivascular stem cells for bone regeneration.
Human perivascular stem cells (PSCs) can be isolated in sufficient numbers from multiple tissues for purposes of skeletal tissue engineering. PSCs are a FACS-sorted population of 'pericytes' (CD146+CD34-CD45-) and 'adventitial cells' (CD146-CD34+CD45-), each of which we have previously reported to have properties of mesenchymal stem cells. PSCs, like MSCs, are able to undergo osteogenic differentiation, as well as secrete pro-osteogenic cytokines. In the present protocol, we demonstrate the osteogenicity of PSCs in several animal models including a muscle pouch implantation in SCID (severe combined immunodeficient) mice, a SCID mouse calvarial defect and a femoral segmental defect (FSD) in athymic rats. The thigh muscle pouch model is used to assess ectopic bone formation. Calvarial defects are centered on the parietal bone and are standardly 4 mm in diameter (critically sized). FSDs are bicortical and are stabilized with a polyethylene bar and K-wires. The FSD described is also a critical size defect, which does not significantly heal on its own. In contrast, if stem cells or growth factors are added to the defect site, significant bone regeneration can be appreciated. The overall goal of PSC xenografting is to demonstrate the osteogenic capability of this cell type in both ectopic and orthotopic bone regeneration models.
Dental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, UCLA, USA.
Aaron W
AW
James
Janette N
JN
Zara
Mirko
M
Corselli
Michael
M
Chiang
Wei
W
Yuan
Virginia
V
Nguyen
Asal
A
Askarinam
Raghav
R
Goyal
Ronald K
RK
Siu
Victoria
V
Scott
Min
M
Lee
Kang
K
Ting
Bruno
B
Péault
Chia
C
Soo
D000818:Animals; D001861:Bone Regeneration; D005269:Femur / Q000473:pathology; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D023421:Models, Animal; D020286:Pericytes / Q000166:cytology*; D051381:Rats; D011923:Rats, Nude; D012886:Skull / Q000473:pathology; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds