The diagnostic performance of classical molecular tests used for detecting human papillomavirus.
Cervical samples were evaluated for human papillomavirus (HPV) presence using the hybrid capture-2 (HC2) assay and the polymerase chain reaction (PCR) with three different primer sets (GP5+/6+, MY09/11 and pU1M/2R). PCR results were compared to HC2 and results of all assays were compared to cytological and colposcopy findings. Post-test probability was assessed in individual assays and test combinations. HPV-DNA prevalence was 36.5% with HC2 and 55.2% with PCR. MY09/11 detected HPV-DNA in 38% of samples, GP5+/6+ in 19.1% and pU1M/2R in 16.4%. pU1M/2R and HC2 had the highest concordance (75.31%, k=0.39 in the whole population; 74.1%, k=0.5 in women with abnormal cytology). pU1M/2R had the best diagnostic performance, including optimal post-test probabilities and cervical abnormality detection (individually or in a panel of tests). Women positive for pU1M/2R may be at higher risk of disease progression; the assay performance when combined with a Pap smear in cervical cancer screening programs should be evaluated.
Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia. marina munoz@fidic.org.com
Marina
M
Munoz
Milena
M
Camargo
Sara C
SC
Soto-De Leon
Adriana
A
Rojas-Villarraga
Ricardo
R
Sanchez
Camilo
C
Jaimes
Antonio
A
Perez-Prados
Manuel E
ME
Patarroyo
Manuel A
MA
Patarroyo
D000328:Adult; D002584:Cervix Uteri / Q000821:virology; D003127:Colposcopy / Q000379:methods; D003584:Cytological Techniques / Q000379:methods; D005260:Female; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D055815:Young Adult