Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis.
Intimins and invasins are virulence factors produced by pathogenic Gram-negative bacteria. They contain C-terminal extracellular passenger domains that are involved in adhesion to host cells and N-terminal β domains that are embedded in the outer membrane. Here, we identify the domain boundaries of an E. coli intimin β domain and use this information to solve its structure and the β domain structure of a Y. pseudotuberculosis invasin. Both β domain structures crystallized as monomers and reveal that the previous range of residues assigned to the β domain also includes a protease-resistant domain that is part of the passenger. Additionally, we identify 146 nonredundant representative members of the intimin/invasin family based on the boundaries of the highly conserved intimin and invasin β domains. We then use this set of sequences along with our structural data to find and map the evolutionarily constrained residues within the β domain.
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
James W
JW
Fairman
Nathalie
N
Dautin
Damian
D
Wojtowicz
Wei
W
Liu
Nicholas
N
Noinaj
Travis J
TJ
Barnard
Eshwar
E
Udho
Teresa M
TM
Przytycka
Vadim
V
Cherezov
Susan K
SK
Buchanan
D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D054324:Enterohemorrhagic Escherichia coli / Q000737:chemistry* / Q000378:metabolism / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010957:Plasmids; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D037521:Virulence Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015011:Yersinia pseudotuberculosis / Q000737:chemistry* / Q000378:metabolism / Q000472:pathogenicity
D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D037521:Virulence Factors; C053476:invasin, Yersinia; C073819:eaeA protein, E coli