Response of an environment-sensitive intramolecular charge transfer probe towards solubilization of liposome membranes by a non-ionic detergent: association and dissociation kinetics.
The present report describes an endeavor to follow the solubilization of DMPC and DMPG liposome membranes by a non-ionic detergent Triton X-100 on the lexicon of environment-sensitive intramolecular charge transfer (ICT) photophysics of an extrinsic molecular probe 5-(4-dimethylamino-phenyl)-penta-2, 4-dienoic acid methyl ester (DPDAME). The prospective applicability of the probe to function as a reporter for detergent-sequestered solubilization of liposome membranes is argued on the basis of comparison of the spectral properties of the probe in various environments. Fluorescence anisotropy study delineates the degree of motional restriction imposed on the probe in different microheterogeneous assemblies. The kinetics of association of the probe with the liposome membranes and the dissociation kinetics of TX-100-sequestered solubilization process of the liposomes have been monitored by the stopped-flow fluorescence technique and the results are rationalized in relevance to fluorescence anisotropy study.
Department of Chemistry, University of Calcutta, 92 A.P.C. Road, Calcutta 700009, India.
Bijan Kumar
BK
Paul
Nikhil
N
Guchhait
D000042:Absorption; D003902:Detergents / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004583:Electrons; D004777:Environment; D005454:Fluorescence Polarization; D007477:Ions; D007700:Kinetics; D008027:Light; D008081:Liposomes / Q000737:chemistry*; D008567:Membranes, Artificial; D008744:Methylamines / Q000737:chemistry; D008823:Micelles; D015335:Molecular Probes / Q000737:chemistry*; D009391:Nephelometry and Turbidimetry; D010316:Particle Size; D010421:Pentanoic Acids / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D012995:Solubility; D013050:Spectrometry, Fluorescence
C551375:5-(4-dimethylamino-phenyl)-penta-2,4-dienoic acid methyl ester; D003902:Detergents; D007477:Ions; D008081:Liposomes; D008567:Membranes, Artificial; D008744:Methylamines; D008823:Micelles; D015335:Molecular Probes; D010421:Pentanoic Acids; D010715:Phosphatidylglycerols; C551282:polyethylene glycol monooctylphenyl ether; D011092:Polyethylene Glycols; C002773:dimyristoylphosphatidylglycerol; D004134:Dimyristoylphosphatidylcholine