Analysis of bacterial polyhydroxybutyrate production by multimodal nanoimaging.
In this paper, we will employ two microscopy techniques, transmission electron microscopy and infrared nanospectromicroscopy, to study the production of polyhydroxybutyrate in Rhodobacter capsulatus and to evaluate the influence of glucose and acetone on the production yield. The results overlap which leads us to a consistent conclusion, highlighting that each technique brings specific and complementary information. By using electron microscopy and infrared nanospectromicroscopy we have proved that both glucose and acetone had a positive effect on the biopolymer production, although the first study done by Fourier transform infrared spectroscopy only identified the effect of acetone. In conclusion, we have now established a method to be able to perform fast diagnostic for PHB production.
Laboratoire de Chimie Physique, Université Paris-Sud, 91405 ORSAY, France.
Céline
C
Mayet
Ariane
A
Deniset-Besseau
Rui
R
Prazeres
Jean-Michel
JM
Ortega
Alexandre
A
Dazzi
D000096:Acetone / Q000378:metabolism / Q000494:pharmacology; D001431:Bacteriological Techniques; D003470:Culture Media; D004867:Equipment Design; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006885:Hydroxybutyrates / Q000032:analysis* / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods*; D011091:Polyesters / Q000032:analysis* / Q000378:metabolism*; D016354:Rhodobacter capsulatus / Q000187:drug effects / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D000094122:Polyhydroxybutyrates
D000096:Acetone; D003470:Culture Media; D005947:Glucose; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates