Construction and preliminary  characterization of a river buffalo bacterial artificial  chromosome library.
River  buffalo genome analyses have advanced significantly in the last decade, and  the genome sequence of Bubalus bubalis will be available shortly.  Nonetheless, large-insert DNA library resources such as bacterial artificial  chromosomes (BAC) are still required for validation and accurate assembly of  the genome sequence. We constructed a river buffalo BAC library containing  52,224 clones with an average insert size of 97 kb, representing 1.7 × coverage  of the genome. This genomic resource for river buffalo will facilitate further  studies in this economically important species allowing for instance, whole  genome physical mapping and isolation of genes and gene clusters, contributing  to the elucidation of gene organization and identification of regulatory  elements.
Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual de São Paulo "Júlio de Mesquita Filho", São José do Rio Preto, SP, Brazil.
N B
NB
Stafuzza
C A
CA
Abbey
C A
CA
Gill
J E
JE
Womack
M E J
ME
Amaral
D000818:Animals; D020029:Base Pairing / Q000235:genetics; D002020:Buffaloes / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D015723:Gene Library; D008967:Molecular Biology / Q000379:methods*; D045483:Rivers