Long time monitoring of the respiratory activity of isolated mitochondria.
We have investigated the ability of optical oxygen sensors incorporated in a microplate to determine the respiratory activity of cell fractions. Different cell fractions were monitored, in particular to evaluate the long term functionality of isolated mitochondria. It is possible to continuously sense respiratory activity of isolated mitochondria over time. We found that they are functional for three hours but stop respiring at a critical limit of 20% air saturation in the system. Furthermore, inhibition and enhancement of respiratory activity were detected. In conclusion, oxygen sensors are a powerful tool to evaluate the functionality of isolated mitochondria.
Department of Pharmaceutical Technology, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany. anne.heller@chemie.uni-regensburg.de
Anne
A
Heller
Lorenz H
LH
Fischer
Otto S
OS
Wolfbeis
Achim
A
Goepferich
D000818:Animals; D016466:CHO Cells; D019069:Cell Respiration / Q000502:physiology; D002469:Cell Separation; D006224:Cricetinae; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption; D013997:Time Factors