Genetic instability and CpG methylation in the 5'-flanking region of the PAI-1 gene in  Chinese patients with gastric cancer.
We explored a possible correlation of genetic  instability and CpG methylation in the 5'-flanking region of the PAI-1 gene  with clinicopathologic features of gastric cancer in Chinese patients and  looked for molecular markers for diagnosing gastric tumor development.  Microsatellite instability and loss of heterozygosity of the PAI-1 gene locus D7S515, D7S471 and pai-1 in 50 specimens of gastric cancer and relevant  pericancerous tissues were detected by PCR-single strand conformation  polymorphism (PCR-SSCP) with sliver staining. Methylation-specific PCR was used  to detect CpG methylation in the 5'-flanking region of the PAI-1 gene.  Microsatellite instability was significantly more common in the negative than  in the positive serosa infiltration group of gastric cancer (42.86 vs 2.33%).  The frequency of microsatellite instability was significantly lower in the  cases with lymph node metastasis than in those without metastasis (18.18 vs 2.56%);  however, it was significantly higher in the low differentiation group than that  in the middle or high differentiation groups (21.05 vs 0.00%). CpG  methylation in the 5'-flanking region of the PAI-1 gene did not differ  significantly. Microsatellite instability and loss of heterozygosity of the  PAI-1 gene apparently regulates the development of gastric cancer through  different pathways. Microsatellite instability could be used as a molecular  marker for the development of gastric cancer. CpG methylation in the  5'-flanking region of the PAI-1 gene appears not to be involved in the  development of gastric cancer.
Institute of Cell Biology, Zhejiang University, Hangzhou, China.
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Yang
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D024506:5' Flanking Region / Q000235:genetics*; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D020022:Genetic Predisposition to Disease; D042822:Genomic Instability / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics; D053842:Microsatellite Instability; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D017395:Plasminogen Activator Inhibitor 1; C051321:SERPINE1 protein, human