Bioconjugation of green fluorescent protein via an unexpectedly stable cyclic sulfonium intermediate.
Smooth converter: Bioconjugation of superfolder GFP involving the formation of an unusually stable, and unprecedented, cyclic sulfonium species is described. This sulfonium can undergo smooth reaction with a range of nucleophiles to give sulfur-, selenium- and azide-modified GFP derivatives in high conversions.
Department of Chemistry, University College London, London, WC1H 0AJ, UK.
Ramiz
R
Nathani
Paul
P
Moody
Mark E B
ME
Smith
Richard J
RJ
Fitzmaurice
Stephen
S
Caddick
D003545:Cysteine; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D013452:Sulfonium Compounds / Q000737:chemistry*
D013452:Sulfonium Compounds; D049452:Green Fluorescent Proteins; D003545:Cysteine