Microfluidity mapping using fluorescence correlation spectroscopy: a new way to investigate plasma membrane microorganization of living cells.
Diffusion time distribution analysis has been employed to highlight the microfluidity fingerprint of plasma membrane of living cells. Diffusion time measurements were obtained through fluorescence correlation spectroscopy performed at the single cell level, over various eukaryotic cell lines (MCF7, LR73, KB3.1, MESSA and MDCKII). The nonsymmetric profile of the diffusion time distributions established experimentally, is discussed according to Monte Carlo simulations, which reproduce the diffusion of the fluorescent probe in heterogeneous membrane.
Université de Technologie de Troyes, Troyes cedex, France.
Pascale
P
Winckler
Aurélie
A
Cailler
Régis
R
Deturche
Pierre
P
Jeannesson
Hamid
H
Morjani
Rodolphe
R
Jaffiol
D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability; D006801:Humans; D009010:Monte Carlo Method; D013050:Spectrometry, Fluorescence / Q000379:methods*