Effect of giardiasis combined with low-protein diet on intestinal absorption of glucose and electrolytes in gerbils.
Studies have shown that symptomatic infection by Giardia lamblia causes acute or chronic diarrhea, dehydration, abdominal pain and malabsorption, leading to undernutrition and weight loss. The aim of the present study was to evaluate the effects of giardiasis and its combination with a low-protein diet on the intestinal absorption of glucose and electrolytes in gerbils. The intestinal absorption of glucose, sodium and potassium was investigated in male gerbils weighing 46-64 g (n≥5). A Tyrode solution containing twice the glucose, sodium and potassium concentration (pH 7.4) was infused through the intestinal loops for 40 min. Glucose absorption was not significantly affected by diet and infection. However, there was a significant increase in sodium absorption in the Giardia-infected group (57.2±6.1, p<0.05) in comparison to the control, low-protein diet and low-protein diet+Giardia-infected groups (8.9±6.5, 2.8±11.1 and 0.8±7.9, respectively; p<0.05). Moreover, potassium was absorbed in the Giardia-infected group (0.45±0.30), while the other groups exhibited potassium secretion. A low-protein diet and Giardia infection had no influence over glucose absorption. However, Giardia infection increased sodium and potassium uptake, suggesting a compensatory mechanism for maintaining homeostasis after likely hypernatremia and hypokalemia caused by the diarrhea that accompanies giardiasis.
Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Maria Aparecida
MA
Gomes
Dirce Ribeiro
DR
de Oliveira
Sabrina Emanuele
SE
de Freitas
Marcelo
M
de Pinho Viana
Elizabeth Lage
EL
Borges
D000818:Animals; D018753:Diet, Protein-Restricted; D004573:Electrolytes / Q000378:metabolism*; D005849:Gerbillinae; D005873:Giardiasis / Q000150:complications / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007408:Intestinal Absorption / Q000502:physiology*; D008297:Male; D011188:Potassium / Q000378:metabolism; D011502:Protein-Energy Malnutrition / Q000150:complications / Q000378:metabolism*; D011897:Random Allocation; D012964:Sodium / Q000378:metabolism
D004573:Electrolytes; D012964:Sodium; D005947:Glucose; D011188:Potassium