Interaction between a plasma membrane-localized ankyrin-repeat protein ITN1 and a nuclear protein RTV1.
The increased tolerance to NaCl 1 (ITN1) protein is a plasma membrane (PM)-localized protein involved in responses to NaCl stress in Arabidopsis. The predicted structure of ITN1 is composed of multiple transmembrane regions and an ankyrin-repeat domain that is known to mediate protein-protein interactions. To elucidate the molecular functions of ITN1, we searched for interacting partners using a yeast two-hybrid assay, and a nuclear-localized DNA-binding protein, RTV1, was identified as a candidate. Bimolecular fluorescence complementation analysis revealed that RTV1 interacted with ITN1 at the PM and nuclei in vivo. RTV1 tagged with red fluorescent protein localized to nuclei and ITN1 tagged with green fluorescent protein localized to PM; however, both proteins localized to both nuclei and the PM when co-expressed. These findings suggest that RTV1 and ITN1 regulate the subcellular localization of each other.
Department of Bioproduction, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri-shi, Hokkaido 093-2422, Japan.
Hikaru
H
Sakamoto
Keiko
K
Sakata
Kensuke
K
Kusumi
Mikiko
M
Kojima
Hitoshi
H
Sakakibara
Koh
K
Iba
D000595:Amino Acid Sequence; D017089:Ankyrin Repeat; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D019441:Plant Epidermis / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C574949:ITN1 protein, Arabidopsis; D008565:Membrane Proteins; D009687:Nuclear Proteins; C574948:RTV1 protein, Arabidopsis; D012097:Repressor Proteins; C461388:VRN1 protein, Arabidopsis