Analysis of native kidney structures in three dimensions.
Optical Projection Tomography (OPT) is an imaging technique, which has proven to be ideally suited to the observation and quantification of kidney development in rodents. Unlike confocal microscopy systems, OPT is capable of imaging the organ in toto across a long window of embryonic development at sufficient resolution to capture relative changes in branching dynamics, pelvis development, and nephrogenesis. Here, we describe how to image kidneys by OPT, and initial steps to quantify kidney development from this data.
Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia. e.kieran.short@monash.edu
Kieran M
KM
Short
Ian M
IM
Smyth
D000818:Animals; D004210:Dissection / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007668:Kidney / Q000254:growth & development* / Q000648:ultrastructure*; D051379:Mice; D013194:Staining and Labeling / Q000379:methods; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods; D041622:Tomography, Optical / Q000379:methods*