Adherence of platelets to in situ albumin-binding surfaces under flow conditions: role of surface-adsorbed albumin.
Surfaces that preferentially bind human serum albumin (HSA) were generated by grafting albumin-binding linear peptide (LP1) onto silicon surfaces. The research aim was to evaluate the adsorption pattern of proteins and the adhesion of platelets from platelet-poor plasma and platelet-rich plasma, respectively, by albumin-binding surfaces under physiological shear rate (96 and 319 s(-1)) conditions. Bound proteins were quantified using enzyme-linked immunosorbent assays (ELISAs) and two-dimensional gel electrophoresis. A ratio of ∼1000:100:1 of adsorbed HSA, human immunoglobulin (HIgG) and human fibrinogen (HFib) was noted, respectively, on LP1-functionalized surfaces, and a ratio of ∼5:2:1 of the same was noted on control surfaces, as confirmed by ELISAs. The surface-adsorbed von Willebrand factor was undetectable by sensitive ELISAs. The amount of adhered platelets correlated with the ratio of adsorbed HSA/HFib. Platelet morphology was more rounded on LP1-functionalized surfaces when compared to control surfaces. The platelet adhesion response on albumin-binding surfaces can be explained by the reduction in the co-adsorption of other plasma proteins in a surface environment where there is an excess of albumin molecules, coupled with restrictions in the conformational transitions of other surface-adsorbed proteins into hemostatically active forms.
Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Sanjukta
S
Guha Thakurta
Robert
R
Miller
Anuradha
A
Subramanian
D000327:Adsorption; D000418:Albumins / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D001792:Blood Platelets / Q000166:cytology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004867:Equipment Design; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007136:Immunoglobulins / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010973:Platelet Adhesiveness; D053657:Platelet-Rich Plasma / Q000378:metabolism; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
D000418:Albumins; D001672:Biocompatible Materials; D007136:Immunoglobulins; D010455:Peptides; D012709:Serum Albumin; D005340:Fibrinogen; D012825:Silicon