Expression of integrin alphavbeta6 in the intestinal epithelial cells of patients with inflammatory bowel disease.
OBJECTIVE
The prevalence of inflammatory bowel disease (IBD) is about 0.05% in industrialized countries. The pathogenesis of IBD remains to be further understood. The present study aims to elucidate the expression of integrin αvβ6 in the intestinal mucosa of patients with IBD.


METHODS
Colonic biopsy was obtained from a group of IBD patients. The expression of αvβ6 in the intestinal mucosa was detected by Western blotting. Human colonic epithelial cell line T84 cells were stimulated by microbial antigen flagellin. The expression of αvβ6 in T84 cells was evaluated by quantitative RT-PCR and Western blotting.


RESULTS
The levels of αvβ6 in the intestinal mucosa were much lower than it in normal control subjects. The serum levels of myeloperoxidase (MPO) were higher in IBD patients that were negatively correlated with the levels of αvβ6 in the intestinal mucosa. The expression of αvβ6 was detectable in T84 cells at naοve status that could be upregulated by exposure to microbial antigen flagellin. Pretreatment with MPO dramatically suppressed the expression of αvβ6 in T84 cells.


CONCLUSIONS
We conclude that the expression of αvβ6 was suppressed in IBD intestinal mucosa, which could be resulted from the high levels of MPO.
Brain Body Institute, McMaster University, Hamilton, ON, Canada.
Bai-Sui
BS
Feng
Xiao
X
Chen
Ping
P
Li
Peng-Yuan
PY
Zheng
Jasmine
J
Chong
Dan-Bi
DB
Cho
Shao-Heng
SH
He
Shang-Guo
SG
Tang
Ping-Chang
PC
Yang