Trypanosoma cruzi: seroprevalence detection in suburban population of Santiago de Querétaro (Mexico).
OBJECTIVE
To evaluate the potential of iron-oxide dismutase excreted (SODeCRU) by T. cruzi as the antigen fraction in the serodiagnosis of Chagas disease and compile new epidemiological data on the seroprevalence of this disease in the suburban population of the city of Santiago de Querétaro (Mexico).


METHODS
258 human sera were analyzed by the techniques of ELISA and Western blot and using the homogenate and the SODeCRU.


RESULTS
A total of 31 sera were positive against ELISA/SODeCRU (12.4%), while 30 sera proved positive by WB/SODeCRU (11.6%). The comparison between the technique of ELISA and WB showed a sensitivity of 93%, and a specificity of 99%. The positive predictive value was 93% and the negative predictive value was 99%, with a Kappa (κ) value of 1.


CONCLUSIONS
These preliminary data reveal the degree of infection of nonrural areas of Mexico and demonstrated that SODeCRU is an antigen useful to diagnose Chagas disease.
Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Severo Ochoa s/n, 18071 Granada, Spain.
Ángeles
Á
López-Céspedes
Elena
E
Villagrán
Kervin
K
Briceño Álvarez
José Antonio
JA
de Diego
Hebert Luís
HL
Hernández-Montiel
Carlos
C
Saldaña
Manuel
M
Sānchez-Moreno
Clotilde
C
Marín
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000913:Antibodies, Protozoan / Q000097:blood; D015153:Blotting, Western; D014355:Chagas Disease / Q000097:blood* / Q000453:epidemiology* / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012107:Research Design; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D012698:Serologic Tests; D013383:Suburban Population; D013696:Temperature; D014349:Trypanosoma cruzi / Q000378:metabolism*
D000913:Antibodies, Protozoan