Different effects of sonoporation on cell morphology and viability.
The objective of our study was to investigate changes in cell morphology and viability after sonoporation. Sonoportion was achieved by ultrasound (21 kHz) exposure on adherent human prostate cancer DU145 cells in the cell culture dishes with the presence of microbubble contrast agents and calcein (a cell impermeant dye). We investigated changes in cell morphology immediately after sonoporation under scanning electron microscope (SEM) and changes in cell viability immediately and 6 h after sonoporation under fluorescence microscope. It was shown that various levels of intracellular calcein uptake and changes in cell morphology can be caused immediately after sonoporation: smooth cell surface, pores in the membrane and irregular cell surface. Immediately after sonoporation, both groups of cells with high levels of calcein uptake and low levels of calcein uptake were viable; 6 h after sonoporation, group of cells with low levels of calcein uptake still remained viable, while group of cells with high levels of calcein uptake died. Sonoporation induces different effects on cell morphology, intracellular calcein uptake and cell viability.
Department of Ultrasound in Medicine, Shanghai Jiao Tong University Affiliated 6th People's Hospital, Shanghai Institute of Ultrasound in Medicine, 600 Yi Shan Road, Shanghai 200233, China.
Ji-Zhen
JZ
Zhang
Jasdeep K
JK
Saggar
Zhao-Li
ZL
Zhou
Bing
B
Hu
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology*; D002477:Cells / Q000648:ultrastructure*; D003470:Culture Media; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D008297:Male; D045423:Microbubbles; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D013010:Sonication; D014465:Ultrasonics
D003470:Culture Media; D005452:Fluoresceins; C007740:fluorexon