Compromised autophagy by MIR30B benefits the intracellular survival of Helicobacter pylori.
Helicobacter pylori evade immune responses and achieve persistent colonization in the stomach. However, the mechanism by which H. pylori infections persist is not clear. In this study, we showed that MIR30B is upregulated during H. pylori infection of an AGS cell line and human gastric tissues. Upregulation of MIR30B benefited bacterial replication by compromising the process of autophagy during the H. pylori infection. As a potential mechanistic explanation for this observation, we demonstrate that MIR30B directly targets ATG12 and BECN1, which are important proteins involved in autophagy. These results suggest that compromise of autophagy by MIR30B allows intracellular H. pylori to evade autophagic clearance, thereby contributing to the persistence of H. pylori infections.
Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing, China.
Bin
B
Tang
Na
N
Li
Jiang
J
Gu
Yuan
Y
Zhuang
Qian
Q
Li
Hai-Guang
HG
Wang
Yao
Y
Fang
Bo
B
Yu
Jin-Yu
JY
Zhang
Qing-Hua
QH
Xie
Li
L
Chen
Xue-Jun
XJ
Jiang
Bin
B
Xiao
Quan-Ming
QM
Zou
Xu-Hu
XH
Mao
D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy; D000071192:Autophagy-Related Protein 12; D001483:Base Sequence; D000071186:Beclin-1; D002460:Cell Line; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics; D016481:Helicobacter Infections / Q000235:genetics / Q000382:microbiology; D016480:Helicobacter pylori / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D042541:Intracellular Space / Q000382:microbiology*; D008565:Membrane Proteins / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D050296:Microbial Viability; D008969:Molecular Sequence Data; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics
C466624:ATG12 protein, human; D051017:Apoptosis Regulatory Proteins; D000071192:Autophagy-Related Protein 12; C491997:BECN1 protein, human; D000071186:Beclin-1; C546086:MIRN30b microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D025841:Small Ubiquitin-Related Modifier Proteins