Type I hair cell regeneration induced by Math1 gene transfer following neomycin ototoxicity in rat vestibular sensory epithelium.
CONCLUSIONS
In the current study, hair cells of vestibular terminal organs in rats were completely eliminated with trans-scala vestibuli injection of neomycin, and then the Math1 gene was transferred. It was shown that type I vestibular hair cells were regenerated and synapses were formed.


OBJECTIVE
The objective of this study was to identify the cell type of the regenerated vestibular hair cells and relative innervation and synaptic linkage after hair cells of vestibular terminal organs in rats were completely eliminated.


METHODS
Neomycin injection was used to eliminate all the vestibular terminal organs, and then the animals were treated with an injection of Ad-Math1-EGFP in the scala vestibuli of the cochlea.


RESULTS
Math1 gene transfer into the inner ear induced type I hair cell regeneration and synaptic formation. However, neither the number nor the appearance of the hair cells was normal.
Department of Otolaryngology Head and Neck Surgery, Institute of Otolaryngology, Chinese PLA General Hospital, Beijing, China.
Jin-Cao
JC
Xu
De-liang
DL
Huang
Zhao-Hui
ZH
Hou
Wei-Wei
WW
Guo
Jian-He
JH
Sun
Li-Dong
LD
Zhao
Ning
N
Yu
Wie-Yen
WY
Young
David Zhi-Zhou
DZ
He
Shi-Ming
SM
Yang
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D018069:Hair Cells, Vestibular / Q000502:physiology* / Q000648:ultrastructure; D006319:Hearing Loss, Sensorineural / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D017348:Microscopy, Electron, Scanning Transmission; D008938:Mitosis; D009355:Neomycin / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*
D051792:Basic Helix-Loop-Helix Proteins; D009355:Neomycin; C500943:Atoh1 protein, mouse