Hematopoietic progenitor constituents and adherent cell progenitor morphology isolated from black-rumped agouti (Dasyprocta prymnolopha, Wagler 1831) bone marrow.
Stem cells are present in the adult tissues of most diverse species. Bone marrow is recognized to be the most exploited site to obtain stem cells and cell progenitors. The objective of the present study was to characterize hematopoietic progenitor (HP) morphology and analyze the performance of adherent cell progenitors (ACPs) cultivated in vitro from black-rumped agouti bone marrow (Dasyprocta prymnolopha). Bone marrow aspirates were obtained from tibia crest and used to prepare histological slides and identify cell morphology. Cells were also scattered on culture plates for later isolation, expansion, and quantification. Smears obtained from bone marrow demonstrated HPs at different stages of maturity. In culture, these cells showed fibroblastoid morphology and a strong tendency to form colonies, demonstrated by the presence of cell aggregates, cytoplasmic elongations lying side by side. An 80% cell confluence was observed at 18 days in culture and progressive reduction in the percentage of nonadherent mononuclear cells. After eight passes, a mean cell viability of 96.07% was observed, from a pool of 1.6 × 10(7) cells (ACP). Thirteen 25-cm(2) culture bottles were trypsinized, resuspended in freezing medium, stored in 14 criotubes at a concentration of 1 × 10(6) cells per milliliter, and placed in liquid nitrogen at -196°C. Agouti bone marrow demonstrated high plasticity, moreover different HP lines, and a population of adherent cells demonstrated morphology similar to mesenchymal stem cells in culture.
Animal Science Post-Graduate Program, Federal University of Piauí (UFPI), Teresina, Piauí, Brazil.
Andressa Rego
AR
Da Rocha
Flávio Ribeiro
FR
Alves
Napoleão Martins
NM
Argôlo Neto
Luciano Fonseca
LF
Dos Santos
Hatawa Melo
HM
De Almeida
Yulla Klinger Pereira
YK
De Carvalho
Dayseanny De Oliveira
Dde O
Bezerra
Maíra Soares
MS
Ferraz
Gerson Tavares
GT
Pessoa
Maria Acelina Martins
MA
De Carvalho
D000818:Animals; D001853:Bone Marrow; D002470:Cell Survival; D006412:Hematopoietic Stem Cells / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008853:Microscopy; D012377:Rodentia