Thauera humireducens sp. nov., a humus-reducing bacterium isolated from a microbial fuel cell.
A Gram-negative, rod-shaped, non-spore-forming bacterium, designated SgZ-1(T), was isolated from the anode biofilm of a microbial fuel cell. The strain had the ability to grow under anaerobic condition via the oxidation of various organic compounds coupled to the reduction of anthraquione-2,6-disulfonate (AQDS) to anthrahydroquinone-2,6-disulfonate (AHQDS). Growth occurred in TSB in the presence of 0-5.5 % (w/v) NaCl (optimum 0-1 %), at 10-45 °C (optimum 25-37 °C) and at pH 6.0-10.0 (optimum 8.0-8.5). Based on 16S rRNA gene sequence similarity, strain SgZ-1(T) belonged to the genus Thauera. The highest level of 16S rRNA gene sequences similarity (96.7 %) was found to be with Thauera aminoaromatica S2(T) and Thauera selenatis AX(T), and lower values were obtained when compared with other recognized Thauera species. Chemotaxonomic analysis revealed that strain SgZ-1(T) contained Q-8 as the predominant quinone, and putrescine and 2-hydroxyputrescine as the major polyamines. The major cellular fatty acids (>5 %) were C16 : 1ω6c and/or C16 : 1ω7c (44.6 %), C16 : 0 (18.8 %), and C18 : 1ω6c and/or C18 : 1ω7c (12.7 %). Based on its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain SgZ-1(T) ( = KACC 16524(T) = CCTCC M 2011497(T)) was designated the type strain of a novel species of the genus Thauera, for which the name Thauera humireducens sp. nov. was proposed.
Guangdong Institute of Eco-Environmental and Soil Science, Guangzhou 510650, PR China.
Gui-Qin
GQ
Yang
College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 PR China.|Guangdong Institute of Eco-Environmental and Soil Science, Guangzhou 510650, PR China.
Jun
J
Zhang
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, RDA, Suwon 441-707, Republic of Korea.
Soon-Wo
SW
Kwon
Guangdong Institute of Eco-Environmental and Soil Science, Guangzhou 510650, PR China.
Shun-Gui
SG
Zhou
Guangdong Institute of Eco-Environmental and Soil Science, Guangzhou 510650, PR China.
Lu-Chao
LC
Han
Guangdong Institute of Eco-Environmental and Soil Science, Guangzhou 510650, PR China.
Ming
M
Chen
Guangdong Institute of Eco-Environmental and Soil Science, Guangzhou 510650, PR China.
Chen
C
Ma
Guangdong Institute of Eco-Environmental and Soil Science, Guangzhou 510650, PR China.
Li
L
Zhuang
D015373:Bacterial Typing Techniques; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D018441:Biofilms; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D011700:Putrescine / Q000031:analogs & derivatives / Q000032:analysis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil; D020628:Thauera / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012987:Soil; C002952:2-hydroxyputrescine; D011700:Putrescine