Recovery of Gram-negative bacilli in stored endotracheal aspirates.
This study assessed the recovery rates of Gram-negative bacilli from stored endotracheal aspirates frozen with and without glycerol. Samples frozen with glycerol showed a significant difference in isolate recovery, 89.7% versus 69.2% (P = 0.02). This study demonstrates that it is possible to achieve high recovery rates of potentially pathogenic organisms from endotracheal aspirates when stored with glycerol, thus broadening the scope of active surveillance cultures for both clinical and research purposes.
Departments of Pathology, School of Medicine, University of Maryland, Baltimore, USA.
Tarah D
TD
Ranke
Paula
P
Strassle
Anthony D
AD
Harris
Jingkun
J
Zhu
J Kristie
JK
Johnson
D012634:Bodily Secretions / Q000382:microbiology*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D006801:Humans; D050296:Microbial Viability / Q000528:radiation effects; D013048:Specimen Handling / Q000379:methods*; D014132:Trachea / Q000382:microbiology*
D003451:Cryoprotective Agents; D005990:Glycerol