[Comparison of different methods of molecular-genetic analysis of somatic mutations in K-ras gene in patients with colorectal cancer].
Two approaches to somatic point mutations in 12 and 13 codones of K-ras gene were analyzed: PCR/SSCP/ACRS/sequencing and allele-specific PCR in the real-life regimen (Russian set "KRAS-7M"). The comparison was carried out on 62 examples of genomic DNA extracted from frozen colon carcinomas, which underwent manual dissection. The results obtained in two attempts were consistent in 95,2% (N=59). Specificity and sensitivity of K-ras mutations detection using "KRAS-7M" set were 100 and 96,4% respectively, and 94,1 and 100% respectievly using PCR/SSCP/ACRS/automatic sequencing. False positive results were absent when detecting with "KRAS-7M" and accounted for 2 cases (5,9%) when using PCR/SSCP/ ACRS/automatic sequencing. The only false negative response (3,6%) was obtained analyzing mutations using "KRAS-7M".
F A
FA
Amosenko
I V
IV
Karpov
A V
AV
Poliakov
S P
SP
Kovalenko
V A
VA
Shamanin
L N
LN
Liubchenko
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis / Q000379:methods / Q000592:standards; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D011905:Genes, ras; D005820:Genetic Testing / Q000379:methods / Q000592:standards; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods / Q000592:standards; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
D004273:DNA, Neoplasm