Development and validation of a reversed-phase HPLC method for determination of alkaloids from Papaver somniferum L. (Papaveraceae).
An HPLC method for the separation of six target alkaloids from Papaver somniferum L. (morphine, codeine, oripavine, thebaine, papaverine, and noscapine) was developed, optimized, and validated. The chromatographic behavior of these alkaloids was investigated using a reversed-phase chromatography at acidic and alkaline pH. The effects of ion-pairing agents, pH value of the mobile phase, concentration of the buffer components, mobile phase organic modifier, and column temperature were studied. Regardless of the large differences in their pKa values, all alkaloids were separated within a close retention window, and good peak shape was achieved for each of the six alkaloids. The proposed method has adequate selectivity, linearity, accuracy, precision, and reproducibility and is applicable for poppy straw.
University Ss. Cyril and Methodius, Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Vodnjanska 17, 1000 Skopje, Republic of Macedonia. jpetrusevska@yahoo.com
Jelena
J
Acevska
Aneta
A
Dimitrovska
Gjoshe
G
Stefkov
Katerina
K
Brezovska
Marija
M
Karapandzova
Svetlana
S
Kulevanova
D000470:Alkaloids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010207:Papaver / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000470:Alkaloids