Muscle carbonic anhydrase III levels in normal and muscular dystrophia afflicted chickens.
BACKGROUND
The levels and immunohistochemical localization of muscle carbonic anhydrase III (CA-III) in healthy chickens and in muscular dystrophia affected (DA) chickens show that the muscles of diseased animal undergo a progressive increase of enzyme activity.


METHODS
An enzyme-linked immunoassay was used to assess the CA-III levels in the muscles and other tissues from eight normal White Leghorn chickens and in two chickens with muscular dystrophy. Immunohistochemical localization of the enzyme in the muscles of these animals was also determined.


RESULTS
The levels of CA-III in the tensor fasciae latae and the superficial pectoral muscles of the DA chickens were higher than the level in normal chickens. The concentrations of CA-III in erythrocytes and plasma from diseased chickens were approximately 15-fold and 1.4-fold higher than in the normal chickens, respectively. In the superficial pectoral and the tensor fasciae latae muscles of diseased chickens, the numbers of strongly stained and weakly stained fibers were greater than that in the normal chickens.


CONCLUSIONS
The levels of CA-III in the superficial pectoral muscle, the tensor fasciae latae muscle, plasma and erythrocytes from the chickens with muscular dystrophy were higher than found in normal chickens.
Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu, University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan. nishida@azabu-u.ac.jp
Toshiho
T
Nishita
Daisuke
D
Yorifuji
Kensuke
K
Orito
Nobutsune
N
Ichihara
Kazuyoshi
K
Arishima
D000818:Animals; D024402:Carbonic Anhydrase II / Q000378:metabolism; D024403:Carbonic Anhydrase III / Q000378:metabolism*; D002645:Chickens / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology*; D009137:Muscular Dystrophy, Animal / Q000201:enzymology*
D024402:Carbonic Anhydrase II; D024403:Carbonic Anhydrase III