[Localization of point mutations in the coding part of the VHL gene in clear cell renal cancer].
VHL gene is often inactivated in sporadic clear cell renal cancer (CCRC) due to somatic mutations, and it's germline mutations cause hereditary CCRC--von Hippel-Lindau syndrome. Localization of mutations in VHL, identification of new mutations and their influence on CCRC progression and sensitivity to targeted therapy are actual problems in modern oncogenetics. We have provided search and characterization of mutations in 248 primary CCRC using SSCP-analysis and sequencing. Somatic mutations were detected in 37.5% of samples, 72% of mutations were identified for the first time. New missense-mutations were analyzed by alignment programs and three-dimensional structure modeling. Mutation frequency was compared in different groups of patients in respect to stage, grade, and metastases. It was demonstrated that 39.1% samples with stage I harbor somatic mutations, however, no association with progression or metastases was found. We also have investigated localization of mutations in the VHL coding part and positions of missense-mutations and inframe deletions/insertions focusing on VHL critical sequences. VHL mutation analysis performed in this study improve the possibilities of laboratory diagnostics of familial and sporadic CCRC.
D S
DS
Mikhaĭlenko
M V
MV
Grigor'eva
I G
IG
Rusakov
R V
RV
Kurynin
A M
AM
Popov
M V
MV
Peters
V B
VB
Matveev
E S
ES
Iakovlev
D A
DA
Nosov
L N
LN
Liubchenko
S A
SA
Tiuliandin
V V
VV
Strel'nikov
D V
DV
Zaletaev
D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D018450:Disease Progression; D056726:Genetic Association Studies; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D016366:Open Reading Frames / Q000235:genetics; D017354:Point Mutation / Q000235:genetics*; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D013329:Structure-Activity Relationship; D051794:Von Hippel-Lindau Tumor Suppressor Protein / Q000235:genetics*; D006623:von Hippel-Lindau Disease / Q000235:genetics* / Q000473:pathology
D051794:Von Hippel-Lindau Tumor Suppressor Protein; C497446:VHL protein, human