Embryonic cell ablation in Drosophila using lasers.
Cell ablation is a powerful tool in the study of eukaryotic developmental biology. The selective removal of cells by ablation may provide much information about their origin, fate, or function in the developing organism. Laser-based techniques have an advantage over genetic or chemical ablation methods in that the operations can be performed in essentially any cell pattern and at any time in development. This protocol describes the methods needed to target and ablate specific cells of interest in Drosophila embryos with lasers.
Sean T
ST
Sweeney
Alicia
A
Hidalgo
J Steven
JS
de Belle
Haig
H
Keshishian
D055011:Ablation Techniques / Q000379:methods*; D000818:Animals; D004330:Drosophila / Q000196:embryology*; D004772:Entomology / Q000379:methods*; D007834:Lasers