Asymptomatic encephalitis in calves experimentally infected with bovine herpesvirus-5.
This study demonstrated that bovine herpesvirus 5 (BoHV)-5 infected calves can develop encephalitis and remain asymptomatic. Seven calves were infected intranasally and monitored for 30 days. Cerebrospinal fluid (CSF) analysis was performed from the onset of neurological signs. Multiple sections of brain and the trigeminal ganglion were submitted to histopathology. Virus detection (PCR and isolation) was performed on CSF and tissues. Four calves developed signs of neurologic disease and died. Three calves remained asymptomatic and were euthanized 30 days post-infection. Cerebrospinal fluid mononuclear pleocytosis occurred in symptomatic and asymptomatic calves. BoHV-5 was isolated and viral DNA was detected in multiple areas of the encephalon of all calves. The viral DNA was detected in the CSF of 2 calves showing neurological signs. Histologically, inflammation was noted in the brain of all calves and confirmed that the encephalitis caused by BoHV-5 may be mild and asymptomatic.
Department of Veterinary Clinics, Universidade Estadual de Londrina, P.O. Box 6001, 86051-990, Londrina, Paraná, Brazil.
Allan Jürgen
AJ
Isernhagen
Mariana
M
Cosenza
Marcio Carvalho
MC
da Costa
Kerlei Cristina
KC
Médici
Mara Regina Stipp
MR
Balarin
Ana Paula Frederico Rodrigues Loureiro
AP
Bracarense
Amauri Alcindo
AA
Alfieri
Júlio Augusto Naylor
JA
Lisbôa
D000818:Animals; D001921:Brain / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology* / Q000821:virology; D002555:Cerebrospinal Fluid / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D018792:Encephalitis, Viral / Q000473:pathology / Q000662:veterinary*; D006566:Herpesviridae Infections / Q000473:pathology / Q000662:veterinary*; D025881:Herpesvirus 5, Bovine / Q000302:isolation & purification / Q000472:pathogenicity; D008297:Male; D008590:Meningoencephalitis / Q000473:pathology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary
D004279:DNA, Viral