Differential expression of salivary glycoproteins in aggressive and chronic periodontitis.
OBJECTIVE
The aim of this study was to compare the pattern of secretion and the expression of mucin glycoprotein-2 (MG2) and lactoferrin in individuals with or without periodontitis.


METHODS
Five individuals with aggressive periodontitis (APG), 5 with generalized chronic periodontitis (CPG) and 5 without periodontitis (CG) were enrolled after informed consent. Non-stimulated and stimulated submandibular and sublingual saliva was collected and samples analyzed by Western blot probed with specific antibodies.


RESULTS
Stimulated and non-stimulated salivary flow rates did not differ among groups. Western blot analysis revealed that stimulation led to: an increase in MG2 expression in all groups, and to lactoferrin expression in APG and CPG. In non-stimulated saliva, CG exhibited the highest expression of both glycoproteins. In stimulated saliva, CG exhibited the highest expression of MG2, whereas APG the highest of lactoferrin.


CONCLUSIONS
The pattern of secretion of MG2 and lactoferrin in health and disease is complex. Although the present study analyzed samples from a limited number of participants, the reduced expression of MG2 and lactoferrin in APG and CPG under non-stimulated condition, the predominant circumstance of salivary secretion during the day, suggests that these salivary constituents may play a role in the etiopathogenesis of these diseases.
School of Dentistry, Pontifical Catholic University of Minas Gerais, Belo Horizonte, MG, Brazil.
Daniela de Morais
Dde M
Rocha
Elton Gonçalves
EG
Zenóbio
Thomas
T
Van Dyke
Karine Simões
KS
Silva
Fernando Oliveira
FO
Costa
Rodrigo Villamarim
RV
Soares
D000328:Adult; D010520:Aggressive Periodontitis / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000032:analysis* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D055262:Mucin-2 / Q000032:analysis* / Q000378:metabolism; D012463:Saliva / Q000737:chemistry*; D012469:Salivary Glands / Q000378:metabolism*; D012636:Secretory Rate; D013997:Time Factors
D055262:Mucin-2; D007781:Lactoferrin