The identification of significant chromosomal regions correlated with oral tongue cancer progression.
OBJECTIVE
Oral squamous cell carcinoma (OSCC) is associated with substantial mortality and morbidity, and oral tongue squamous cell carcinoma (SCC) is representative in OSCC. Early detection of oral premalignant lesions (OPLs) that will develop into invasive tumors is necessary to improve the poor prognosis of this cancer.


METHODS
To identify potential biomarkers that could be used for early detection, we compared the gene expression of incident primary oral tongue SCC, severe dysplasia, mild and moderate dysplasia. For this, we used three expression datasets obtained from a public database and selected chromosomal locations related with the progress of oral tongue cancer from a dataset. We then evaluated the gene set, which is included in the selected chromosomal locations, using out of bag (OOB) error and plots in two validation datasets.


RESULTS
Sixty-two chromosomal locations were detected, and most genomic aberrations were shown in chromosome 3. We identified 62 genes included in those locations, and three precancerous and SCC groups were well classified with low OOB error rates. These were also discriminative in the two validation datasets.


CONCLUSIONS
The selected probes with genomic alteration in low-grade dysplasia can be used as an effective predictor for disease progression.
Oral Cancer Research Institute, College of Dentistry, Yonsei University, Seoul 120-752, Republic of Korea. kky1004@yuhs.ac
Ki-Yeol
KY
Kim
In-Ho
IH
Cha
D000328:Adult; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D002893:Chromosomes, Human, Pair 3; D018450:Disease Progression; D042241:Early Diagnosis; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology*; D011379:Prognosis; D014062:Tongue Neoplasms / Q000235:genetics* / Q000473:pathology*; D055815:Young Adult
D014408:Biomarkers, Tumor