Trypanosomes contain two highly different isoforms of peroxin PEX13 involved in glycosome biogenesis.
We previously identified the peroxin PEX13 in Trypanosoma brucei. Although lacking some features considered typical of PEX13s, it appeared functional in the biogenesis of glycosomes, the peroxisome-like organelles of trypanosomatids. Here we report the identification of a very different trypanosomatid PEX13, not containing the commonly encountered PEX13 SH3 domain but having other typical features. It is readily detected with the jackhmmer database search program, but not with PSI-BLAST. This is the first time different PEX13 isoforms are reported in a single organism. We show that this PEX13.2, like the PEX13.1 previously described, is associated with glycosomes and that its depletion by RNA interference affects the biogenesis of the organelles and viability of trypanosomes. The features considered typical of PEX13s are discussed.
Research Unit for Tropical Diseases, de Duve Institute and Laboratory of Biochemistry, Université catholique de Louvain, Avenue Hippocrate 74, Postal Box B1.74.01, B-1200 Brussels, Belgium.
Ana
A
Brennand
Daniel J
DJ
Rigden
Paul A M
PA
Michels
D000595:Amino Acid Sequence; D008830:Microbodies / Q000378:metabolism*; D008969:Molecular Sequence Data; D001678:Organelle Biogenesis; D020675:Peroxisomes / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D016415:Sequence Alignment; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000378:metabolism*; D018909:src Homology Domains
D020033:Protein Isoforms; D015800:Protozoan Proteins