Glutathione S-transferase genes and the risk of recurrent miscarriage in Italian women.
OBJECTIVE
To investigate the role of glutathione S-transferases (GSTs) in the pathogenesis of recurrent miscarriage (RM).


METHODS
Genetic association study.


METHODS
University of Rome, Tor Vergata and San Giovanni Calibita, Fatebenefratelli Hospital.


METHODS
One hundred twenty-one women with RM and 113 women without pregnancy complications.


METHODS
Genomic DNA extracted from buccal cells and screening of positive/null genotypes of GSTM1 and GSTT1 genes and single nucleotide polymorphisms of GSTA1, GSTO2, and GSTP1 genes.


METHODS
Occurrence of GST polymorphisms.


RESULTS
Women with at least one GSTA1*-69T allele are more frequent in the RM group than in the control group: 67% vs. 48%, respectively. Significant outcomes were obtained considering different genetic models: codominant, dominant, and log-additive. In addition, the combined analysis suggests that GSTA1 and GSTM1 variants have a significant interaction in RM risk.


CONCLUSIONS
Our study highlighted a significant association between the GSTA1 gene and an increased risk of RM. In particular, the -69T allele in the GSTA1 gene may be considered as a predisposing factor of RM.
Department of Biology, University of Rome Tor Vergata, Rome, Italy.
Renato
R
Polimanti
Sara
S
Piacentini
Natalia
N
Lazzarin
Elena
E
Vaquero
Maria Antonietta
MA
Re
Dario
D
Manfellotto
Maria
M
Fuciarelli
D000026:Abortion, Habitual / Q000201:enzymology / Q000453:epidemiology* / Q000235:genetics*; D000328:Adult; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies / Q000379:methods*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008875:Middle Aged; D011247:Pregnancy; D012307:Risk Factors; D055815:Young Adult
C484555:GSTA1 protein, human; D005982:Glutathione Transferase