Dynamic in situ chromosome immobilisation and DNA extraction using localized poly(N-isopropylacrylamide) phase transition.
A method of in situ chromosome immobilisation and DNA extraction in a microfluidic polymer chip was presented. Light-induced local heating was used to induce poly(N-isopropylacrylamide) phase transition in order to create a hydrogel and embed a single chromosome such that it was immobilised. This was achieved with the use of a near-infrared laser focused on an absorption layer integrated in the polymer chip in close proximity to the microchannel. It was possible to proceed to DNA extraction while holding on the chromosome at an arbitrary location by introducing protease K into the microchannel.
Johan
J
Eriksen
Anil Haraksingh
AH
Thilsted
Rodolphe
R
Marie
Christopher James
CJ
Lüscher
Lars Bue
LB
Nielsen
Winnie Edith
WE
Svendsen
Peter
P
Szabo
Anders
A
Kristensen