Dissociation of embryonic kidney followed by re-aggregation as a method for chimeric analysis.
This chapter presents three methods for re-constructing mouse foetal kidney tissue from simple suspensions of cells. These techniques are very useful for a number of purposes: (1) they allow the production of fine-grained chimaeras in which cell autonomy of mutations can be tested, (2) they provide an environment that allows the renal differentiation potential of stem cells to be assessed, and (3) they are an excellent system in which to study the mechanisms of self-organization. Each of the methods described here begins with disaggregation of embryonic mouse kidneys, followed by re-aggregation and culture; the main differences are in the culture methods, each of which has advantages for particular purposes.
University of Edinburgh, Hugh Robson Building, Edinburgh, UK.
Jamie A
JA
Davies
Mathieu
M
Unbekandt
Jessica
J
Ineson
Michael
M
Lusis
Melissa H
MH
Little
D000818:Animals; D002678:Chimera / Q000254:growth & development*; D016591:Histocytological Preparation Techniques / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D007668:Kidney / Q000166:cytology* / Q000196:embryology*; D051379:Mice; D008853:Microscopy / Q000379:methods; D041681:NIH 3T3 Cells; D009924:Organ Culture Techniques / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*