Oral tissue response to ovine grafting biomaterial: morphological and morphometric study using scanning electron and light microscopy tissue response to ovine grafting biomaterial.
OBJECTIVE
To evaluate the oral tissue response to an experimental particle ovine biomaterial by scanning electron microscopy (SEM) and light microscopy (LM).


METHODS
Forty-eight rats had surgical periodontal defects treated with either blood clotting (control), bovine biomaterial™ (B), or an experimental ovine biomaterial (O). Data from SEM analysis (defect exposure, root surface exposure, diameter of matrix fibers and bundles, and globuli areas; n = 5) were applied to Shapiro-Wilk, Kruskal-Wallis, and Dunn's test, whereas LM analysis (tissue cicatrization characteristics and diameter defect; n = 3) had data applied to two-way analysis of variance. Animals were monitored for 1 and 3 weeks.


RESULTS
By SEM, the O samples showed significant differences from B and C in the area of defect exposure (H(2,15) = 8.66; P < 0.05). In both periods, O and B samples showed similar results for matrix fiber diameters, differently than C samples (H(2,15) = 14.0; P < 0.05). All other SEM variables were considered equivalent among the groups (P > 0.05). Under LM, an acute and chronic granulomatous inflammation was seen in the presence of both biomaterials (B and O, 1 week); both the control and the ovine grafting samples showed mature bone in the repair site (3 weeks); the defect diameter showed similar values among groups, at both monitoring periods (F(2,12) = 1.0401; P > 0.05).


CONCLUSIONS
The ovine particles of this study showed a favorable response to oral tissue repair, demonstrating to be a potential source for the development of bone grafting biomaterials.
Master's Program in Clinical Dentistry, Universidade Positivo, Curitiba, PR 81280-330, Brazil. jzielak2@gmail.com
Joao Cesar
JC
Zielak
Alvaro Luiz
AL
Mathias
Rosiellen
R
Da Silva
Bruno Skronski
BS
Ton
Aline Graziela Cidral
AG
Sobreiro
Allan Fernando
AF
Giovanini
Tatiana Miranda
TM
Deliberador
Flares
F
Baratto Filho
Lucienne Miranda
LM
Ulbrich
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D004195:Disease Models, Animal; D008853:Microscopy / Q000379:methods; D010510:Periodontal Diseases / Q000628:therapy*; D051381:Rats; D012756:Sheep; D016896:Treatment Outcome
D001672:Biocompatible Materials