Synthesis of ¹³C- and ¹⁴C-labeled dinucleotide mRNA cap analogues for structural and biochemical studies.
Herein we describe the first simple and short method for specific labeling of mono- and trimethylated dinucleotide mRNA cap analogues with (13)C and (14)C isotopes. The labels were introduced within the cap structures either at the N7 for monomethylguanosine cap or N7 and N2 position for trimethylguanosine cap. The compounds designed for structural and biochemical studies will be useful tools for better understanding the role of the mRNA cap structures in pre-mRNA splicing, nucleocytoplasmic transport, translation initiation and mRNA degradation.
Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
Karolina
K
Piecyk
Richard E
RE
Davis
Marzena
M
Jankowska-Anyszka
D002247:Carbon Isotopes / Q000737:chemistry; D002250:Carbon Radioisotopes / Q000737:chemistry; D015226:Dinucleoside Phosphates / Q000737:chemistry*; D007553:Isotope Labeling; D012314:RNA Cap Analogs / Q000138:chemical synthesis* / Q000737:chemistry; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*
D002247:Carbon Isotopes; D002250:Carbon Radioisotopes; D015226:Dinucleoside Phosphates; D012314:RNA Cap Analogs; D012333:RNA, Messenger