Use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis for rapid confirmation of Burkholderia pseudomallei in septicemic melioidosis.
Burkholderia pseudomallei was quickly identified from blood cultures collected from septicemic patients by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis using an in-house reference library. This procedure reduced the time to definitive identification by more than 24 hours. This analysis is a useful addition to laboratory methods for early recognition of septicemic melioidosis in non-endemic settings.
School of Pathology and Laboratory Medicine, Faculty of Medicine, Dentistry, and Health Sciences, University of Western Australia, Nedlands 6009, Western Australia, Australia. tim.inglis@health.wa.gov.au
Timothy J J
TJ
Inglis
Paul E
PE
Healy
Leith J
LJ
Fremlin
Clayton L
CL
Golledge
D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001315:Australia; D016957:Burkholderia pseudomallei / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D008297:Male; D008554:Melioidosis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology*; D000077731:Meropenem; D008875:Middle Aged; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013845:Thienamycins / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D013845:Thienamycins; D000077731:Meropenem