SCAR markers for discriminating species of two genera  of medicinal plants, Liriope and Ophiopogon.
The development of DNA markers that can closely  discriminate between Liriope and Ophiopogon species is vital for  efficient and accurate identification of these species, and to ensure the  quality, safety, and efficacy of medicines made from these plants. We developed  species-specific molecular markers for these two genera. Forty RAPD primers  were tested to detect polymorphism; species-specific RAPD bands were  gel-purified, cloned, and sequenced. Primers for sequence-characterized  amplified regions (SCARs) were then designed, based on nucleotide sequences of  specific RAPD primers. SCAR markers SA06 and SB05, specific to Ophiopogon  japonicus, amplified 460- and 553-bp DNA fragments, respectively. The  marker SA12 amplified a 485-bp fragment specific to Liriope platyphylla.  This is the first report of a species-specific SCAR marker for this group.  These markers will be useful for rapid identification of closely related Liriope and Ophiopogon species.
Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, Korea.
G
G
Li
Y-J
YJ
Park
D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics; D031424:Liriope Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000235:genetics; D031441:Ophiopogon / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D018744:DNA, Plant; D005819:Genetic Markers; D009711:Nucleotides