Aptamer-nanoparticle assembly for logic-based detection.
In this work, gold nanoparticles perform Boolean logic operations in response to two proangiogenic targets important in cancer diagnosis and treatment: PDGF and VEGF. In the absence of protein target, gold nanoparticles are initially dispersed as a red solution; the addition of target proteins causes nanoparticle aggregation, turning the solution blue, as well as the release of dye-labeled aptamer probes, which causes an increase in fluorescence. These outputs constitute an AND or OR gate for simultaneous protein detection. We believe this logic-gate-based detection system will become the basis for novel rapid, cheap, and reliable sensors for diagnostic applications.
Center for Research at Bio/nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, University of Florida , Gainesville, Florida 32611-7200, United States.
Mohammed I
MI
Shukoor
Meghan O
MO
Altman
Da
D
Han
Abdullah Tahir
AT
Bayrac
Ismail
I
Ocsoy
Zhi
Z
Zhu
Weihong
W
Tan