The chemical composition of endotoxin isolated from intestinal strain of Desulfovibrio desulfuricans.
Desulfovibrio desulfuricans anaerobes are constituents of human alimentary tract microflora. There are suggestions that they take part in the pathogenesis of periodontitis and some gastrointestinal inflammatory disorders, such as ulcerative colitis or Crohn's disease. Endotoxin is one of Gram-negative bacteria cellular components that influence these microorganisms pathogenicity. Endotoxin is a lipid-polisaccharide heteropolymer consisting of three elements: lipid A, core oligosaccharide, and O-specific polysaccharide, also called antigen-O. The biological activity of lipopolysaccharide (LPS) is determined by its structure. In this study, we show that rhamnose, fucose, mannose, glucose, galactose, heptose, and 2-keto-3-deoxyoctulosonic acid (Kdo) are constituents of D. desulfuricans endotoxin oligosaccharide core and O-antigen. Lipid A of these bacteria LPS is composed of glucosamine disaccharide substituted by 3-acyloxyacyl residues: ester-bound 3-(dodecanoyloxy)tetradecanoic, 3-(hexadecanoyloxy)tetradecanoic acid, and amide-bound 3-(tetradecanoyloxy)tetradecanoic acid.
Department of Biochemistry, Faculty of Pharmacy, Medical University of Silesia, Narcyzow 1 street, 41-200 Sosnowiec, Poland. jlodowska@sum.edu.pl
Jolanta
J
Lodowska
Daniel
D
Wolny
Marzena
M
Jaworska-Kik
Sławomir
S
Kurkiewicz
Zofia
Z
Dzierżewicz
Ludmiła
L
Węglarz
D045285:Desulfovibrio desulfuricans / Q000145:classification* / Q000378:metabolism*; D004731:Endotoxins / Q000096:biosynthesis / Q000737:chemistry*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000737:chemistry*; D013045:Species Specificity
D004731:Endotoxins; D008070:Lipopolysaccharides