Discovery of a trans-dichloroethene-respiring Dehalogenimonas species in the 1,1,2,2-tetrachloroethane-dechlorinating WBC-2 consortium.
The WBC-2 consortium is an organohalide-respiring anaerobic microbial enrichment culture capable of dechlorinating 1,1,2,2-tetrachloroethane (TeCA) to ethene. In the WBC-2 culture, TeCA is first transformed to trans-dichloroethene (tDCE) by dichloroelimination; tDCE is subsequently transformed to vinyl chloride (VC) and then to ethene by hydrogenolysis. Analysis of 16S rRNA gene clone libraries from culture DNA revealed sequences from three putative dechlorinating organisms belonging to Dehalococcoides, Dehalobacter, and Dehalogenimonas genera. Quantitative PCR primers were designed for each of these sequences, and their abundance was quantified in enrichment cultures over time. These data revealed that complete dechlorination of TeCA to ethene involves all three organisms. Dehalobacter spp. grew during the dihaloelimination of TeCA to tDCE, while Dehalococcoides and Dehalogenimonas spp. grew during hydrogenolysis of tDCE to ethene. This is the first time a genus other than Dehalococcoides has been implicated in dechlorination of tDCE to VC.
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada.
Marie J
MJ
Manchester
Laura A
LA
Hug
Matt
M
Zarek
Anna
A
Zila
Elizabeth A
EA
Edwards
D041862:Chloroflexi / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002849:Chromatography, Gas; D003470:Culture Media / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004980:Ethane / Q000031:analogs & derivatives* / Q000378:metabolism; D015723:Gene Library; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity
D003470:Culture Media; D017931:DNA Primers; D006843:Hydrocarbons, Chlorinated; D012336:RNA, Ribosomal, 16S; C015530:1,1,2,2-tetrachloroethane; D004980:Ethane