Two methods to easily obtain nucleotide sequences from AFLP loci of interest.
Genome scans based on anonymous Amplified Fragment Length Polymorphism (AFLP) markers scattered throughout the genome are becoming an increasingly popular approach to study the genetic basis of adaptation and speciation in natural populations. A shortcoming of this approach is that despite its efficiency to detect signatures of selection, it can hardly help pinpoint the specific genomic region(s), gene(s), or mutation(s) targeted by selection. Here, we present two methods to be undertaken after performing an AFLP-based genome scan to easily obtain the sequences of AFLP loci detected as outliers by population genomics approaches. The first one is based on the gel excision of the target AFLP fragment, after simplification of the AFLP fingerprint and separation of the fragments by migration. The second one is a combination of classical AFLP protocol and 454 pyrosequencing.
Institute of Integrative Biology, ETH Zurich, Zurich, Switzerland. margotparis1@gmail.com
Margot
M
Paris
Claire-Lise
CL
Meyer
Christelle
C
Blassiau
Eric
E
Coissac
Pierre
P
Taberlet
Laurence
L
Després
D000222:Adaptation, Physiological / Q000235:genetics; D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D000818:Animals; D001419:Bacteria; D001483:Base Sequence / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005658:Fungi; D056426:Genetic Loci; D049810:Genetic Speciation; D016678:Genome; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D010944:Plants; D011110:Polymorphism, Genetic; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*