The effects of different sugars on motility, morphology and DNA damage during the liquid storage of rat epididymal sperm at 4°C.
This study evaluated the protective effects of supplementation with three different sugars on the motility, morphology and DNA integrity of rat epididymal sperm chilled and stored at 4°C Epididymides were obtained from each donor. Rat epididymal sperm was diluted in Ham's F10 plus raffinose, Ham's F10 plus trehalose, Ham's F10 plus fructose, and Ham's F10 medium for control purposes. Thereafter, the extended sperm were chilled and stored in liquid form at 4°C. Sperm motility, morphological abnormalities and DNA damage were determined at 0 and 12h after chilling. No significant difference was observed in any of the parameters evaluated at 0h, before storage (P>0.05). After 12h of storage, all sugar additives led to statistically higher motility, normal sperm morphology and DNA integrity in comparison to the control group. Raffinose gave the best motility percentages (32.86±1.84%) after 12h of storage at 4°C, compared to the other groups (P<0.001). In conclusion, Raffinose, trehalose and fructose provided a better protection of sperm functional parameters against chilling injury, in comparison to the control group.
Erciyes University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Kayseri, Turkey. sariozkan75@yahoo.com
Serpil
S
Sariözkan
Mustafa Numan
MN
Bucak
Fazile
F
Canturk
Saim
S
Özdamar
Arzu
A
Yay
Pürhan Barbaros
PB
Tuncer
Servet
S
Özcan
Neslihan
N
Sorgucu
Yusuf
Y
Caner
D000818:Animals; D003080:Cold Temperature; D020552:Comet Assay; D004247:DNA / Q000235:genetics; D004249:DNA Damage; D004822:Epididymis / Q000166:cytology* / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D008297:Male; D011887:Raffinose / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism
D005632:Fructose; D004247:DNA; D014199:Trehalose; D011887:Raffinose