Characterization of a novel IMP-28 metallo-β-lactamase from a Spanish Klebsiella oxytoca clinical isolate.
An isolate of Klebsiella oxytoca carrying a novel IMP metallo-β-lactamase was discovered in Madrid, Spain. The bla(IMP-28) gene is part of a chromosomally located class I integron. The IMP-28 k(cat)/K(m) values for ampicillin, ceftazidime, and cefepime and, to a lesser extent, imipenem and meropenem, are clearly lower than those of IMP-1. The His306Gln mutation may induce important modifications of the L3 loop and thus of substrate accessibility and hydrolysis and be the main reason for this behavior.
Servicio de Microbiología-INIBIC, Complejo Hospitalario Universitario A Coruña, A Coruña, Spain.
Francisco José
FJ
Pérez-Llarena
Ana
A
Fernández
Laura
L
Zamorano
Frédéric
F
Kerff
Alejandro
A
Beceiro
Belén
B
Aracil
Emilia
E
Cercenado
Elisenda
E
Miro
Antonio
A
Oliver
Jesús
J
Oteo
Ferran
F
Navarro
Germán
G
Bou
D000595:Amino Acid Sequence; D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D000077723:Cefepime; D002442:Ceftazidime / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D006657:Histones / Q000235:genetics; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D032023:Integrons / Q000235:genetics*; D041121:Klebsiella oxytoca / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D016415:Sequence Alignment; D013030:Spain; D013845:Thienamycins / Q000494:pharmacology; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002511:Cephalosporins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D006657:Histones; D013845:Thienamycins; C074160:BlaA protein, Bacteria; D015378:Imipenem; D000667:Ampicillin; D000077723:Cefepime; D002442:Ceftazidime; D001618:beta-Lactamases; D000077731:Meropenem