Alveolar macrophages in neonatal mice are inherently unresponsive to Pneumocystis murina infection.
Pneumocystis pneumonia was first diagnosed in malnourished children and has more recently been found in children with upper respiratory symptoms. We previously reported that there is a significant delay in the immune response in newborn mice infected with Pneumocystis compared to adults (Garvy BA, Harmsen AG, Infect. Immun. 64:3987-3992, 1996, and Garvy BA, Qureshi M, J. Immunol. 165:6480-6486, 2000). This delay is characterized by the failure of neonatal lungs to upregulate proinflammatory cytokines and attract T cells into the alveoli. Here, we report that regardless of the age at which we infected the mice, they failed to mount an inflammatory response in the alveolar spaces until they were 21 days of age or older. Anti-inflammatory cytokines had some role in dampening inflammation, since interleukin-10 (IL-10)-deficient pups cleared Pneumocystis faster than wild-type pups and the neutralization of transforming growth factor beta (TGF-β) with specific antibody enhanced T cell migration into the lungs at later time points. However, the clearance kinetics were similar to those of control pups, suggesting that there is an intrinsic deficiency in the ability of innate immunity to control Pneumocystis. We found, using an adoptive transfer strategy, that the lung environment contributes to association of Pneumocystis organisms with alveolar macrophages, implying no intrinsic deficiency in the binding of Pneumocystis by neonatal macrophages. Using both in vivo and in vitro assays, we found that Pneumocystis organisms were less able to stimulate translocation of NF-κB to the nucleus of alveolar macrophages from neonatal mice. These data indicate that there is an early unresponsiveness of neonatal alveolar macrophages to Pneumocystis infection that is both intrinsic and related to the immunosuppressive environment found in neonatal lungs.
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky, USA.
Cathryn
C
Kurkjian
Melissa
M
Hollifield
J Louise
JL
Lines
Amy
A
Rogosky
Kerry M
KM
Empey
Mahboob
M
Qureshi
Stephen A
SA
Brown
Beth A
BA
Garvy
D000818:Animals; D000831:Animals, Newborn; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D005786:Gene Expression Regulation / Q000276:immunology; D007113:Immunity, Innate / Q000502:physiology*; D007074:Immunoglobulin G / Q000032:analysis / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D008198:Lymph Nodes; D016676:Macrophages, Alveolar / Q000382:microbiology*; D051379:Mice; D008815:Mice, Inbred Strains; D011010:Pneumocystis / Q000145:classification*; D016720:Pneumocystis Infections / Q000276:immunology / Q000382:microbiology*; D011650:Pulmonary Alveoli / Q000382:microbiology; D013601:T-Lymphocytes / Q000502:physiology; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
D007074:Immunoglobulin G; D016212:Transforming Growth Factor beta; D016753:Interleukin-10