Detection of allo-HLA cross-reactivity by virus-specific memory T-cell clones using single HLA-transfected K562 cells.
The ability to directly measure virus-specific lymphocytes using fluorochrome-labeled tetrameric complexes has proven a great advancement for the transplantation field. Viral peptide/HLA tetrameric complexes allow the rapid generation of virus-specific clones using single cell sorting apparatus, permitting the determination of alloreactivity from a single TCR with known specificity. When combined with new target "detector" cells called single HLA antigen-transfected K562 cells (SALs), the human alloresponse can for the first time be examined specifically and reliably. Here we describe a method for detection of "heterologous immunity" from virus-specific memory T-cells using single HLA expressing cell lines as allogeneic targets.
Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands. Lloyd.DOrsogna@health.wa.gov.au
Lloyd J
LJ
D'Orsogna
Ellen M W
EM
van der Meer-Prins
Yvonne M
YM
Zoet
Dave L
DL
Roelen
Ilias I N
II
Doxiadis
Frans H J
FH
Claas
D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002999:Clone Cells; D003429:Cross Reactions / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D020014:K562 Cells; D014162:Transfection; D014780:Viruses / Q000276:immunology*
D006680:HLA Antigens