Characterization of  microsatellite markers in cassava based on microsatellite-AFLP technique.
We  developed molecular markers for cassava based on the microsatellite-amplified  fragment length polymorphism (M-AFLP) technique. Twenty primer pairs were  developed and used for the analysis of 48 samples of Manihot species,  consisting of M. esculenta (33), M. esculenta ssp flabellifolia (3), M. chlorosticta (3), M. carthaginensis (3), M.  filamentosa (3), and M. tristis (3). Nine microsatellite loci that  were polymorphic among these Manihot species were identified, giving 32  polymorphic alleles and from two to seven alleles per locus. Unbiased and  direct count heterozygosity varied from 0.0233 to 0.7924 and 0.0000 to 0.7083,  respectively. There was significant deviation (P < 0.05) from Hardy-Weinberg  equilibrium at five loci. Genotypic data from the Manihot species were  subjected to genetic diversity analysis. We found that M. chlorosticta and M. esculenta ssp flabellifolia were the closest populations,  while M. filamentosa and M. esculenta ssp flabellifolia were  the most divergent. Considering within M. esculenta, the samples from  Nigeria and Fiji were the most closely related, while those from Venezuela and  of unknown origin were the most divergent. We conclude that the M-AFLP  technique is an effective method for generating microsatellite markers that are  useful for genetic diversity analysis in Manihot species.
Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
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Whankaew
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Sraphet
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Thaikert
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Smith
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Triwitayakorn
D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D005838:Genotype; D002365:Manihot / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics