Multiple folding states and disorder of ribosomal protein SA, a membrane receptor for laminin, anticarcinogens, and pathogens.
The human ribosomal protein SA (RPSA) is a multilocus protein, present in most cellular compartments. It is a multifunctional protein, which belongs to the ribosome but is also a membrane receptor for laminin, growth factors, prion, pathogenic microorganisms, toxins, and the anticarcinogen epigallocatechin gallate. It contributes to the crossing of the blood-brain barrier by neurotropic viruses and bacteria and is used as a biomarker of metastasis. RPSA includes an N-terminal domain, which is homologous to the prokaryotic ribosomal proteins S2, and a C-terminal extension, which is conserved in vertebrates. The structure of its N-domain has been determined from crystals grown at 17 °C. The structure of its C-domain remains unknown. We produced in Escherichia coli and purified the full-length RPSA and its N- and C-domains. We characterized the folding states of these recombinant proteins mainly by methods of fluorescence and circular dichroism spectrometry, in association with quantitative analyses of their unfolding equilibria, induced with heat or urea. The necessary equations were derived from first principles. The results showed that the N-domain unfolded according to a three-state equilibrium. The monomeric intermediate was predominant at the body temperature of 37 °C. It also existed in the full-length RPSA and bound ANS, a small fluorescent molecule. The C-domain was in an intrinsically disordered state. The recombinant N- and C-domains weakly interacted together. These results indicated a high plasticity of RPSA, which could be important for its multiple cellular localizations and functional interactions.
Institut Pasteur , Unit of Molecular Prevention and Therapy of Human Diseases, Department of Infection and Epidemiology, rue du Dr. Roux, F-75015 Paris, France.
Mohamed B
MB
Ould-Abeih
Isabelle
I
Petit-Topin
Nora
N
Zidane
Bruno
B
Baron
Hugues
H
Bedouelle
D000595:Amino Acid Sequence; D000818:Animals; D016588:Anticarcinogenic Agents / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D051379:Mice; D008829:Microbiology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D058767:Protein Unfolding / Q000187:drug effects; D017474:Receptors, Laminin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014508:Urea / Q000494:pharmacology
D016588:Anticarcinogenic Agents; D007797:Laminin; C510624:RPSA protein, human; D017474:Receptors, Laminin; D012269:Ribosomal Proteins; D014508:Urea