Pseudonocardia antitumoralis sp. nov., a deoxynyboquinone-producing actinomycete isolated from a deep-sea sediment.
An aerobic actinomycete, designated SCSIO 01299(T), was isolated from a deep-sea sediment collected from the northern South China Sea at a depth of 3258 m. The isolate was found to be a natural producer of the synthesized antitumour agent deoxynyboquinone and its three new derivatives, pseudonocardians A, B and C. A blast search based on almost-complete 16S rRNA gene sequences showed that strain SCSIO 01299(T) had high sequence similarities with members of the genus Pseudonocardia. The 16S rRNA gene sequence phylogenetic tree revealed that strain SCSIO 01299(T) was a member of the genus Pseudonocardia. Phenotypic analysis, chemotaxonomy and DNA-DNA relatedness could readily distinguish the isolate from established members in this genus. It was concluded that strain SCSIO 01299(T) represents a novel species, for which the name Pseudonocardia antitumoralis sp. nov. is proposed. The type strain is SCSIO 01299(T) ( = DSM 45322(T)  = CCTCC M 2011255(T)).
Key Laboratory of Marine Bio-resources Sustainable Utilization, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China.
Xin-Peng
XP
Tian
Key Laboratory of Marine Bio-resources Sustainable Utilization, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China.
Li-Juan
LJ
Long
Key Laboratory of Marine Bio-resources Sustainable Utilization, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China.
Su-Mei
SM
Li
Key Laboratory of Marine Bio-resources Sustainable Utilization, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China.
Jing
J
Zhang
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.
Ying
Y
Xu
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.
Jie
J
He
Key Laboratory of Marine Bio-resources Sustainable Utilization, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China.
Jie
J
Li
Key Laboratory of Marine Bio-resources Sustainable Utilization, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China.
Fa-Zuo
FZ
Wang
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.
Wen-Jun
WJ
Li
Key Laboratory of Marine Bio-resources Sustainable Utilization, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China.
Chang-Sheng
CS
Zhang
Key Laboratory of Marine Bio-resources Sustainable Utilization, CAS; RNAM Center for Marine Microbiology, CAS; Guangdong Key Laboratory of Marine Materia Medica; South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China.
Si
S
Zhang
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; C550580:deoxynyboquinone; D024482:Vitamin K 2; C024223:vitamin MK 8