Construction of an insertion marker collection of Sz. japonicus (IMACS) for genetic mapping and a fosmid library covering its genome.
Measuring relative genetic distances is one of the best ways to locate genetic loci. Here we report the construction of a strains set for genetic mapping in Schizosaccharomyces japonicus, which belongs to the genus Schizosaccharomyces together with the well-studied fission yeast Sz. pombe. We constructed 29 strains that bear a positive-negative selection marker at different loci. The marker was inserted every 500 kb in the genome of Sz. japonicus. Each marker thus becomes a 'scale mark' of a chromosome that behaves like a yardstick. By determining the genetic distances from the inserted markers, the relative location of a genomic mutation can be determined. We also constructed a fosmid library that covers an entire genome of Sz. japonicus. These tools together would facilitate identification and cloning of the gene.
Department of Genetics, Graduate University for Advanced Studies, Sokendai, Japan.
Kanji
K
Furuya
Keita
K
Aoki
Hironori
H
Niki
D002874:Chromosome Mapping / Q000379:methods; D015723:Gene Library; D005827:Genetics, Microbial / Q000379:methods*; D016681:Genome, Fungal; D016254:Mutagenesis, Insertional / Q000379:methods*; D012568:Schizosaccharomyces / Q000235:genetics*; D012641:Selection, Genetic