Ruegeria arenilitoris sp. nov., isolated from the seashore sand around a seaweed farm.
A Gram-negative, motile and rod-shaped bacterial strain, G-M8(T), which was isolated from seashore sand around a seaweed farm at Geoje island in South Korea, was characterized taxonomically. It grew optimally at 30-37 °C, at pH 7.0-8.0 and in presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain G-M8(T) joined the cluster comprising the type strains of Ruegeria atlantica and Ruegeria lacuscaerulensis, showing 97.5 % sequence similarity, by a bootstrap resampling value of 85.8 %. It exhibited 16S rRNA gene sequence similarity values of 95.4-96.7 % to the type strains of the other Ruegeria species. Strain G-M8(T) exhibited the highest gyrB sequence similarity value (88.5 %) to the type strain of R. lacuscaerulensis. Strain G-M8(T) contained Q-10 as the predominant ubiquinone and C(18:1)                 ω7c as the predominant fatty acid. The polar lipid profile of strain G-M8(T) was similar to that of R. atlantica KCTC 12424(T). The DNA G+C content of strain G-M8(T) was 64.6 mol% and its mean DNA-DNA relatedness values with R. atlantica KCTC 12424(T) and R. lacuscaerulensis KCTC 2953(T) were 18 ± 5.3 and 10 ± 3.6 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain G-M8(T) is distinguished from other Ruegeria species. On the basis of the data presented, strain G-M8(T) (=KCTC 23960(T) = CCUG 62412(T)) represents a novel species of the genus Ruegeria, for which the name Ruegeria arenilitoris sp. nov. is proposed.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Sooyeon
S
Park
Jung-Hoon
JH
Yoon
D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012624:Seaweed / Q000254:growth & development; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012965:Sodium Chloride; D012822:Silicon Dioxide