SNP-Ratio Mapping (SRM): identifying lethal alleles and mutations in complex genetic backgrounds by next-generation sequencing.
We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds.
Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich, CH-8008 Zürich, Switzerland.
Heike
H
Lindner
Michael T
MT
Raissig
Christian
C
Sailer
Hiroko
H
Shimosato-Asano
Rémy
R
Bruggmann
Ueli
U
Grossniklaus
D002874:Chromosome Mapping / Q000379:methods*; D005804:Genes, Lethal; D017343:Genes, Plant; D005838:Genotype; D009154:Mutation; D053205:Pollen Tube / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide