Simple procedure for fatty acid analysis of glycerophospholipids in Escherichia coli and Saccharomyces cerevisiae.
Rapid, convenient methods have been developed for fatty acid analysis of membrane glycerophospholipids in microorganisms. Fatty acid methyl esters derived from glycerophospholipids have been prepared directly from wet pellets of Escherichia coli cells or Saccharomyces cerevisiae spheroplasts without lipid extraction and fractionation in high yields under mild temperature conditions for analysis by gas chromatography.
Department of Biomolecular Chemistry, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan. ken-ichi@astem.or.jp
Ken'ichi
K
Ichihara
Atsumi
A
Tani
D005591:Chemical Fractionation; D002849:Chromatography, Gas / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D020404:Glycerophospholipids / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013104:Spheroplasts / Q000737:chemistry; D013696:Temperature
D005227:Fatty Acids; D020404:Glycerophospholipids