Cell-selective metabolic glycan labeling based on ligand-targeted liposomes.
A cell-specific metabolic glycan labeling strategy has been developed using azidosugars encapsulated in ligand-targeted liposomes. The ligands are designed to bind specific cell-surface receptors that are only expressed or up-regulated in target cells, which mediates the intracellular delivery of azidosugars. The delivered azidosugars are metabolically incorporated into cell-surface glycans, which are then imaged via a bioorthogonal reaction.
Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Ran
R
Xie
Senlian
S
Hong
Lianshun
L
Feng
Jie
J
Rong
Xing
X
Chen
D001386:Azides / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism; D002241:Carbohydrates / Q000008:administration & dosage / Q000032:analysis; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D050356:Lipid Metabolism; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism*; D013194:Staining and Labeling
D001386:Azides; D002241:Carbohydrates; D008081:Liposomes; D011134:Polysaccharides