Gravity-oriented microfluidic device for uniform and massive cell spheroid formation.
We propose a simple method for forming massive and uniform three-dimensional (3-D) cell spheroids in a multi-level structured microfluidic device by gravitational force. The concept of orienting the device vertically has allowed spheroid formation, long-term perfusion, and retrieval of the cultured spheroids by user-friendly standard pipetting. We have successfully formed, perfused, and retrieved uniform, size-controllable, well-conditioned spheroids of human embryonic kidney 293 cells (HEK 293) in the gravity-oriented microfluidic device. We expect the proposed method will be a useful tool to study in-vitro 3-D cell models for the proliferation, differentiation, and metabolism of embryoid bodies or tumours.
Kangsun
K
Lee
Choong
C
Kim
Jae
J
Young Yang
Hun
H
Lee
Byungwook
B
Ahn
Linfeng
L
Xu
Ji
J
Yoon Kang
Kwang W
KW
Oh