The whereabouts of 2D gels in quantitative proteomics.
Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features make it a still highly valuable tool, especially when multiple quantitative comparisons of samples must be made, and even for large samples series. However, quantitative proteomics using 2D gels is critically dependent on the performances of the protein detection methods used after the electrophoretic separations. This chapter therefore examines critically the various detection methods (radioactivity, dyes, fluorescence, and silver) as well as the data analysis issues that must be taken into account when quantitative comparative analysis of 2D gels is performed.
CEA-DSV-iRTSV/CBM and UMR CNRS-UJF 5249, CEA Grenoble, Grenoble, France. thierry.rabilloud@cea.fr
Thierry
T
Rabilloud
D000818:Animals; D002478:Cells, Cultured; D004396:Coloring Agents / Q000737:chemistry; D003627:Data Interpretation, Statistical; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics; D016622:Silver Staining
D004396:Coloring Agents; D005456:Fluorescent Dyes; D020543:Proteome