Production of galanthamine by Leucojum aestivum shoots grown in different bioreactor systems.
The production of galanthamine by shoots of Leucojum aestivum grown in different bioreactor systems (shaking and nonshaking batch culture, temporary immersion system, bubble bioreactor, continuous and discontinuous gassing bioreactor) under different culture conditions was studied. The influence of the nutrient medium, weight of inoculum, and size of bioreactor on both growth and galanthamine production was studied. The maximal yield of galanthamine (19.416 mg) was achieved by cultivating the L. aestivum shoots (10 g of fresh inoculum) in a temporary immersion system in a 1-L bioreactor vessel which was used as an airlift culture vessel, gassing 12 times per day (5 min).
BioPlanta GmbH, Deutscher Platz 5, 04103 Leipzig, Germany. Anika.Schumann@bioplanta-leipzig.de
Anika
A
Schumann
Strahil
S
Berkov
Diana
D
Claus
André
A
Gerth
Jaume
J
Bastida
Carles
C
Codina
D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D005702:Galantamine / Q000096:biosynthesis*; D019668:Liliaceae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
D003470:Culture Media; D005702:Galantamine