Chemical composition, antioxidant and anticancer effects of the seeds and leaves of indigo (Polygonum tinctorium Ait.) plant.
Seeds and leaves of indigo (Polygonum tinctorium Ait.) plant were investigated and compared with another medicinal plant named prolipid for their properties such as chemical composition, antioxidant, and anticancer effects by Fourier transform infrared, three-dimensional fluorescence spectroscopy, and electrospray ionization-MS in negative mode. It was found that polyphenols, flavonoids, and flavanols were significantly higher in prolipid (P<0.05), following by indigo mature leaves, immature leaves, and seeds. Methanol extract of mature indigo leaves in comparison with the ethyl acetate extract showed higher inhibition of proliferation. The interaction between polyphenol extracts of indigo mature leaves and BSA showed that indigo has a strong ability, as other widely used medicinal plants, to quench the intrinsic fluorescence of BSA by forming complexes. In conclusion, indigo mature leaves were compared with prolipid. High content of bioactive compounds, antioxidant, fluorescence, and antiproliferative properties of indigo justifies the use of this plant as a medicinal plant and a new source of antioxidants.
Naju Foundation of Natural Dyeing Culture, Naju 520-931, South Korea.
Hong-Gi
HG
Jang
Buk-Gu
BG
Heo
Yong Seo
YS
Park
Jacek
J
Namiesnik
Dinorah
D
Barasch
Elena
E
Katrich
Kann
K
Vearasilp
Simon
S
Trakhtenberg
Shela
S
Gorinstein
D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D018533:Biomass; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002839:Chromans / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005470:Fluorometry; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared
D000970:Antineoplastic Agents; D000975:Antioxidants; D002839:Chromans; D010936:Plant Extracts; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid