Establishment of mesenchymal cell line derived from human developing odontoma.
OBJECTIVE
An odontoma, which shows proliferating odontogenic epithelium and mesenchymal tissue, is one of the most common odontogenic tumors encountered. These are commonly found in tooth-bearing regions, although the etiology remains unknown. There are no previous reports of an established line of immortalized human odontoma cells.


METHODS
Using odontoma fragments obtained from a girl treated at our department, we established an immortalized human odontoma cell line and investigated cell morphology, dynamic proliferation, the presence of contamination, and karyotype. Moreover, cell characterization was examined using osteogenic and odontogenic markers.


RESULTS
We successfully established a mesenchymal odontoma cell (mOd cells). The cells were found to be fibroblastic and had a high level of telomerase activity. Cell growth was confirmed after more than 200 population doublings without significant growth retardation. mOd cells expressed mRNA for differentiation markers, including collagen type I (COLI), alkaline phosphatase, bone sialoprotein, osteopontin, osteocalcin, cementum-derived protein (CP-23), dentin sialophosphoprotein (DSPP), and distal-less homeobox 3 (DLX3), as well as bone morphogenetic proteins (BMPs). In addition, they showed a high level of calcified nodule formation activity in vitro.


CONCLUSIONS
We successfully established a cell line that may be useful for investigating the mechanisms of normal odontogenesis as well as characteristics of odontoma tumors.
Department of Oral and Maxillofacial Surgery, Division of Cervico-Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
H
H
Hatano
Y
Y
Kudo
I
I
Ogawa
H
H
Shimasue
H
H
Shigeishi
K
K
Ohta
K
K
Higashikawa
M
M
Takechi
T
T
Takata
N
N
Kamata
D000293:Adolescent; D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D019485:Bone Morphogenetic Proteins / Q000032:analysis; D002113:Calcification, Physiologic / Q000502:physiology; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048430:Cell Shape; D002648:Child; D002675:Child, Preschool; D024042:Collagen Type I / Q000032:analysis; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D018398:Homeodomain Proteins / Q000032:analysis; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000032:analysis; D059785:Karyotype; D008648:Mesoderm / Q000473:pathology*; D008875:Middle Aged; D009810:Odontoma / Q000235:genetics / Q000473:pathology*; D015675:Osteocalcin / Q000032:analysis; D053495:Osteopontin / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis; D019098:Telomerase / Q000032:analysis; D014157:Transcription Factors / Q000032:analysis; D055815:Young Adult; D000099311:Dentin Sialophosphoprotein
D000469:Alkaline Phosphatase; D015415:Biomarkers; D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D018398:Homeodomain Proteins; D058574:Integrin-Binding Sialoprotein; D015675:Osteocalcin; D053495:Osteopontin; D010750:Phosphoproteins; D011506:Proteins; D012795:Sialoglycoproteins; D019098:Telomerase; D014157:Transcription Factors; D000099311:Dentin Sialophosphoprotein; C510886:CEMP1 protein, human; C088334:Distal-less homeobox proteins