In vitro anthelmintic activity of five tropical legumes on the exsheathment and motility of Haemonchus contortus infective larvae.
This study investigated the in vitro anthelmintic (AH) activity of five tropical legume plants [Arachis pintoi CIAT 22160 (A.p. 22160), Gliricidia sepium, Cratylia argentea (C.a. Yacapani), C. argentea CIAT 22386 (C.a. 22386), C. argentea Veranera (C.a. Veranera)] against Haemonchus contortus infective larvae and the role of tannins/polyphenolic compounds in the AH effect. Lyophilized leaf extracts of each plant were evaluated using the Larval Exsheathment Inhibition Assay (LEIA) and the larval migration inhibition assay (LMIA). The role of tannins/polyphenolic compounds in the AH effect was evaluated in both assays using polyethylene glycol (PEG) to remove tannins from the solutions. At the highest concentration (1200μg of extract/ml), A. pintoi 22160, C.a. Yacapani, C.a. Veranera and C.a. 22386 completely inhibited the exsheathment process of H. contortus (P<0.01). At the same concentration (1200μg of extract/ml), the inhibition of larval migration for C.a. 22386, C.a. Veranera and G. sepium was 66.0%, 35.9% and 39.2% (relative to the PBS control), respectively. In both bioassays (LEIA and LMIA), the AH effect shown by each plant was blocked after the addition of polyethylene glycol (PEG), corroborating the role of tannins/polyphenolic compounds.
Centro de Enseñanza Investigación y Extensión en Ganadería Tropical, Universidad Nacional Autónoma de México, Km 5.5 Carretera Federal Tlapacoyan-Martínez de la Torre, CP 93600, Veracruz, Mexico.
Elke
E
von Son-de Fernex
Miguel Angel
MA
Alonso-Díaz
Braulio
B
Valles-de la Mora
Concepción M
CM
Capetillo-Leal
D000818:Animals; D000871:Anthelmintics / Q000302:isolation & purification / Q000494:pharmacology*; D001681:Biological Assay; D007887:Fabaceae / Q000737:chemistry*; D006189:Haemonchus / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D012756:Sheep; D013634:Tannins / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology
D000871:Anthelmintics; D010636:Phenols; D010936:Plant Extracts; D013634:Tannins