The bovine oocyte in vitro maturation model: a potential tool for reproductive toxicology screening.
Reproductive toxicity testing according to the present guidelines requires a high number of animals. Therefore, the development of alternative in vitro methods is urgently required. The aim of the present study was to investigate the applicability domain of the bovine oocyte in vitro maturation assay (bIVM) to study female reproductive toxicology. Therefore, bovine oocytes were exposed to a broad set of chemicals of two distinct biological function groups: (a) affecting female fertility and (b) affecting embryonic development and having a broad range of physical and chemical properties. The endpoints evaluated were the oocyte nuclear maturation (progression of meiosis) and general cytotoxicity. The oocyte nuclear maturation was negatively affected by all compounds tested and the effect was observed at concentrations lower than the cytotoxic ones. The bIVM assay correctly predicted the classification of compounds between those predefined groups. Additionally, the bIVM model contributes significantly for the 3R principle, since no test animals are used in this assay. In conclusion, the bIVM is a sensitive and valuable alternative assay to identify potential chemical hazard on female fertility.
TNO, Zeist, The Netherlands. anna.beker@tno.nl
Anna
A
Beker van Woudenberg
Mariska
M
Gröllers-Mulderij
Cor
C
Snel
Nelleke
N
Jeurissen
Rob
R
Stierum
André
A
Wolterbeek
D000818:Animals; D001681:Biological Assay; D002417:Cattle; D048708:Cell Growth Processes / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D015203:Reproducibility of Results; D012098:Reproduction / Q000187:drug effects; D013723:Teratogens / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods*
D013723:Teratogens