Correlative light and scanning X-ray scattering microscopy of healthy and pathologic human bone sections.
Scanning small and wide angle X-ray scattering (scanning SWAXS) experiments were performed on healthy and pathologic human bone sections. Via crystallographic tools the data were transformed into quantitative images and as such compared with circularly polarized light (CPL) microscopy images. SWAXS and CPL images allowed extracting information of the mineral nanocrystalline phase embedded, with and without preferred orientation, in the collagen fibrils, mapping local changes at sub-osteon resolution. This favorable combination has been applied for the first time to biopsies of dwarfism syndrome and Paget's disease to shed light onto the cortical structure of natural bone in healthy and pathologic sections.
Istituto di Cristallografia-IC-CNR, via Amendola 122/O, I-70126 Bari, Italy. cinzia.giannini@ic.cnr.it
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Giannini
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Siliqi
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Bunk
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Beraudi
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Ladisa
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Altamura
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Stea
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Baruffaldi
D000328:Adult; D000369:Aged, 80 and over; D001847:Bone Diseases / Q000473:pathology*; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000648:ultrastructure; D018360:Crystallography, X-Ray; D004392:Dwarfism / Q000473:pathology; D006253:Haversian System / Q000033:anatomy & histology; D006801:Humans; D008027:Light; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008859:Microscopy, Polarization / Q000379:methods; D010001:Osteitis Deformans / Q000473:pathology; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D053838:Scattering, Small Angle; D013577:Syndrome; D014961:X-Ray Diffraction