Treating acute cystitis with biodegradable micelle-encapsulated quercetin.
Intravesical application of an anti-inflammatory drug is an efficient strategy for acute cystitis therapy. Quercetin (QU) is a potent anti-inflammatory agent; however, its poor water solubility restricts its clinical application. In an attempt to improve water solubility of QU, biodegradable monomethoxy poly(ethylene glycol)-poly(ɛ-caprolactone) (MPEG-PCL) micelles were used to encapsulate QU by self-assembly methods, creating QU/MPEG-PCL micelles. These QU/MPEG-PCL micelles with DL of 7% had a mean particle size of <34 nm, and could release QU for an extended period in vitro. The in vivo study indicated that intravesical application of MPEG-PCL micelles did not induce any toxicity to the bladder, and could efficiently deliver cargo to the bladder. Moreover, the therapeutic efficiency of intravesical administration of QU/MPEG-PCL micelles on acute cystitis was evaluated in vivo. Results indicated that QU/MPEG-PCL micelle treatment efficiently reduced the edema and inflammatory cell infiltration of the bladder in an Escherichia coli-induced acute cystitis model. These data suggested that MPEG-PCL micelle was a candidate intravesical drug carrier, and QU/MPEG-PCL micelles may have potential application in acute cystitis therapy.
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Chengdu, People's Republic of China.
Bi Lan
BL
Wang
Xiang
X
Gao
Ke
K
Men
Jinfeng
J
Qiu
Bowen
B
Yang
Ma Ling
ML
Gou
Mei Juan
MJ
Huang
Ning
N
Huang
Zhi Yong
ZY
Qian
Xia
X
Zhao
Yu Quan
YQ
Wei
D000208:Acute Disease; D000704:Analysis of Variance; D000818:Animals; D003556:Cystitis / Q000188:drug therapy* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006651:Histocytochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008823:Micelles; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity; D009929:Organ Size / Q000187:drug effects; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D014018:Tissue Distribution; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism
D008823:Micelles; D053769:Nanocapsules; D011091:Polyesters; C439611:methoxy poly(ethylene glycol-co-epsilon-caprolactone); D011092:Polyethylene Glycols; D011794:Quercetin