Oxygen inhalation improves survival time of mice with acute intra-abdominal hypertension and protects liver cells.
OBJECTIVE
The objectives of this study were to establish a mouse model of sustainable, stable acute intra-abdominal hypertension (IAH), seeking to observe the survival time and liver functions at different intra-abdominal pressures (IAP), and to investigate changes after oxygen therapy.


METHODS
Sixty Kunming mice were assigned to 4 groups with an average of 15, 20, 30, or 40 cmH(2)O IAP. The 40 cmH(2)O group seemed to be appropriate for follow-up experiments. The 45 mice added to the cohort were assigned into 3 groups administered an average of 50%, 80%, and 100% O(2), respectively. Liver and blood samples were used to compare the rates of apoptosis using the TUNEL assay as well as alanine aminotransferase (ALT), aspartate aminotransferase (AST); Caspase-3, 9, MDA, and SOD concentrations.


RESULTS
Most animals in the 15 and 20 cmH(2)O groups survived 8 hours. The average survival for the 30 and 40 cmH(2)O groups were 4.54 ± 0.54 hours and 2.04 ± 0.44 hours, respectively (P < .01). As the oxygen concentration increased, the survival time was prolonged among the 40 cmH(2)O IAP group (P < .01), and the member of apoptotic hepatic cells decreased (P < .01), with a concomittent decrease in caspase 3 and 9 as well as malondialdehyde, although superoxide dismutase showed the opposite results.


CONCLUSIONS
The present work using a mouse model for acute IAH showed oxygen inhalation to improve host survival and protect liver cells.
General Surgery Department, The 180th Hospital of People's Liberation Army, Quanzhou, People's Republic of China.
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Q
He
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L
Cai
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S
Zhang
Y
Y
Chen
G
G
Liu
C
C
Zhang
D000208:Acute Disease; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D019610:Cytoprotection; D004195:Disease Models, Animal; D000860:Hypoxia / Q000097:blood / Q000473:pathology / Q000628:therapy*; D020287:In Situ Nick-End Labeling; D059325:Intra-Abdominal Hypertension / Q000097:blood / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010102:Oxygen Inhalation Therapy; D011312:Pressure; D013050:Spectrometry, Fluorescence; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D015415:Biomarkers; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C505409:Casp3 protein, mouse; C506434:Casp9 protein, mouse; D053148:Caspase 3; D053453:Caspase 9