Lactobacillus protected bone damage and maintained the antioxidant status of liver and kidney homogenates in female wistar rats.
The aim of the study was to evaluate protective property of Lactobacillus casei and Lactobacillus acidophilus in minimizing oxidative stress associated with arthritis from liver and kidney. Subsequently, protective property of Lactobacillus against the bone damage was also taken into consideration. Arthritis was induced by injecting freund's complete adjuvant (100 μl) into sub plantar surface of hind paw. Oral administration of culture, vehicle, and drug started after induction of arthritis (i.e. on day 9th). Indomethacin was used as a standard drug. Radiographic analysis of the hind paw knee joint was also done at the end of the 21st day. Oxidative stress parameters were studied from liver and kidney homogenate. Histopathology of liver and kidney was also performed. Lactobacillus treatment significantly rescued the enzymatic level of catalase, superoxide dismutase, reduced glutathione, and glutathione peroxidase in both liver and kidney homogenates, whereas it has decreased the malonaldehyde accumulation. Oral administration of Lactobacillus also significantly decreased the serum ceruloplasmin level. Radiographic analysis also corroborated these findings. Lactobacillus treatment maintained the normal histopathology of liver and kidney. Results of this study clearly suggest that L. casei and L. acidophilus, alone or in combination, decreased the bone damaged and effectively restored antioxidant status of liver and kidney. Lactobacillus has a significant antiarthritic and antioxidant activity against freund's complete adjuvant induced arthritis in rats.
Department of Microbiology, Barkatullah University, Bhopal 462026, Madhya Pradesh, India. ananyasarika@gmail.com
Sarika
S
Amdekar
Avnish
A
Kumar
Poonam
P
Sharma
Rambir
R
Singh
Vinod
V
Singh
D000276:Adjuvants, Immunologic / Q000009:adverse effects / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001168:Arthritis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005620:Freund's Adjuvant / Q000009:adverse effects / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology / Q000473:pathology; D007779:Lactobacillus acidophilus; D007780:Lacticaseibacillus casei; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000276:Adjuvants, Immunologic; D000975:Antioxidants; D005620:Freund's Adjuvant; D010088:Oxidoreductases; D005978:Glutathione