Virus-induced gene silencing using begomovirus satellite molecules.
Virus-induced gene silencing (VIGS) has emerged as a powerful method for studying gene function. VIGS is induced by infecting a plant with a plant virus that has had its genome modified to include a sequence from the host gene to be silenced. DNAβ and DNA1 are satellite and single-stranded DNA molecules associated with begomoviruses (family Geminiviridae). We converted DNAβ and DNA1 into gene-silencing vectors. The VIGS vectors can induce silencing efficiently in many solanaceous plants. Here, we describe procedures for the use of these two gene-silencing vectors for VIGS in different hosts.
Institute of Biotechnology, Zhejiang University, Hangzhou, China. zzhou@zju.edu.cn
Xueping
X
Zhou
Changjun
C
Huang
D053839:Begomovirus / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005822:Genetic Vectors; D018551:Solanum lycopersicum / Q000235:genetics / Q000821:virology; D032306:Petunia / Q000235:genetics / Q000821:virology; D034622:RNA Interference; D014026:Nicotiana / Q000235:genetics / Q000821:virology
D004276:DNA, Satellite; D004279:DNA, Viral