Effect of preservation conditions of collagen substrate on its fibril formation and rabbit chondrocyte morphology.
The degree of collagen fibril formation was altered by varying the preservation conditions. The collagen substrate under atmosphere of nitrogen gas for 4 days exhibited well-developed collagen fibril network accompanied with the frequency of round-shaped chondrocyte cells (f(R)) of 0.62, the value of which was slightly lower than that on the reference substrate. The exposure to air and prolongation of preservation led to further degradation of collagen fibril networks accompanied with f(R) lower than 0.4. It was thus demonstrated that the preservation conditions of the collagen substrate influenced the chondrocyte cell morphology.
Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Masrina Mohd
MM
Nadzir
Masahiro
M
Kino-oka
Katsura
K
Sugawara
Masahito
M
Taya
D000818:Animals; D048430:Cell Shape / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D024042:Collagen Type I / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000187:drug effects / Q000648:ultrastructure; D024022:Fibrillar Collagens / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D009584:Nitrogen / Q000494:pharmacology; D011817:Rabbits; D023822:Tissue Engineering
D024042:Collagen Type I; D024022:Fibrillar Collagens; D009584:Nitrogen