[Establishment of a keloid model by transplanting human keloid onto the backs of nude mice].
Keloid scar is a proliferative healing dysfunction formed by an excessive build-up of collagen fibers on the dermis. It is responsible of aesthetic and functional disabilities. There is no ideal treatment and recurrence occurs very often. Keloid scars occur only to human, that's why animal model needs to be made to study this pathology and new treatments. Few models have been described using human keloid scars implanted into subcutaneous tissue of nude mice or rat. To allow study of topical and laser treatment we have developed a new animal model using human keloid scar fragment with epidermal and dermal tissue implanted into back of nude mice like a full thickness skin graft. Keloid fragments from five donors have been grafted onto 40 nudes mice. Macroscopic and microscopic studies have been made at day 28, 56, 84 and 112. We observed integration of the fragments in all cases. Hyalinized collagen bundles were observed in all implant biopsies confirming the stability of the keloid architecture within 112 days. This model is easily reproducible and allows the study of topical treatment and laser due to the accessibility of the keloid.
Service de chirurgie plastique et maxillofacial, hôpital Nord de Marseille, chemin des Bourrely, 13915 Marseille cedex, France; Inserm U 703, 152, rue du Dr.-Yersin, 59120 Loos, France. Electronic address: cecile.philandrianos@ap-hm.fr.
C
C
Philandrianos
Service de chirurgie plastique et maxillofacial, hôpital Nord de Marseille, chemin des Bourrely, 13915 Marseille cedex, France.
D
D
Gonnelli
Laboratoire d'anatomie-pathologique, hôpital Nord de Marseille, chemin des Bourrely, 13915 Marseille cedex, France.
L
L
Andrac-Meyer
Laboratoire d'anatomie-pathologique, hôpital Nord de Marseille, chemin des Bourrely, 13915 Marseille cedex, France.
M
M
Bruno
Service de chirurgie plastique, hôpital de la Conception, 147, boulevard Baille, 13905 Marseille, France.
G
G
Magalon
Inserm U 703, 152, rue du Dr.-Yersin, 59120 Loos, France.
S
S
Mordon
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007627:Keloid / Q000601:surgery; D051379:Mice; D008819:Mice, Nude; D014183:Transplantation, Heterologous