[A method for induction of mouse CD8α(+);CD11b(+);jagged2(high);regulatory dendritic cells with mesenchymal stem cells in vitro].
OBJECTIVE
To develop a method for induction of the mouse bone marrow-derived CD8α(+);CD11b(+);jagged2(high);regulatory dendritic cells (DCregs) using mesenchymal stem cells (MSCs) in vitro.


METHODS
BALB/c(H-2(d);)mouse bone marrow cells (BMCs) were isolated and induced to CD8α(+);CD11c(+);CD11b(+);DCs (DCs) by 4 cytokines in vitro for 3 d. The DCs were selected by flow cytometry (FCM),  and co-cultured with MSCs for 10 d to get DCregs. Immunophenotypes,  cell cycle,  Jagged1 and Jagged2 ligands of the Notch pathway of DCs were analyzed by FCM before and after co-culture.


RESULTS
The novel DCs were transformed into DCregs successfully. The expressions of CD86,  CD80,  CD40,  and MHC-II significantly decreased (P<0.05),  while those of CD205,  Jagged1 and Jagged2 obviously increased on DCregs (P<0.05). Meanwhile,  the percentage of treated MSCs cells went up in G2 and S phases.


CONCLUSIONS
MSCs co-cultured with DCs can induce the development of DCregs,  which have immune tolerance-associated phenotypes and higher proliferation ability. This mechanism might be related to the up-regulated Jagged1 and Jagged2 expressions and the T-cell Notch pathway activation.
Department of Haematology, Guangdong Academy of Medical Sciences, Guangzhou, China.
Jian-yu
JY
Weng
Xin
X
Huang
Pei-long
PL
Lai
Yue-ying
YY
Li
Lin
L
Xu
Ping
P
Wu
Su-xia
SX
Geng
Xin
X
DU
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism*; D016827:CD8 Antigens / Q000378:metabolism*; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D016130:Immunophenotyping; D000072101:Jagged-2 Protein; D008565:Membrane Proteins / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C
D039481:CD11b Antigen; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; C490503:Jag2 protein, mouse; D000072101:Jagged-2 Protein; D008565:Membrane Proteins