Caffeine relaxes smooth muscle through actin depolymerization.
Caffeine is sometimes used in cell physiological studies to release internally stored Ca(2+). We obtained evidence that caffeine may also act through a different mechanism that has not been previously described and sought to examine this in greater detail. We ruled out a role for phosphodiesterase (PDE) inhibition, since the effect was 1) not reversed by inhibiting PKA or adenylate cyclase; 2) not exacerbated by inhibiting PDE4; and 3) not mimicked by submillimolar caffeine nor theophylline, both of which are sufficient to inhibit PDE. Although caffeine is an agonist of bitter taste receptors, which in turn mediate bronchodilation, its relaxant effect was not mimicked by quinine. After permeabilizing the membrane using β-escin and depleting the internal Ca(2+) store using A23187, we found that 10 mM caffeine reversed tone evoked by direct application of Ca(2+), suggesting it functionally antagonizes the contractile apparatus. Using a variety of molecular techniques, we found that caffeine did not affect phosphorylation of myosin light chain (MLC) by MLC kinase, actin-filament motility catalyzed by MLC kinase, phosphorylation of CPI-17 by either protein kinase C or RhoA kinase, nor the activity of MLC-phosphatase. However, we did obtain evidence that caffeine decreased actin filament binding to phosphorylated myosin heads and increased the ratio of globular to filamentous actin in precontracted tissues. We conclude that, in addition to its other non-RyR targets, caffeine also interferes with actin function (decreased binding by myosin, possibly with depolymerization), an effect that should be borne in mind in studies using caffeine to probe excitation-contraction coupling in smooth muscle.
Firestone Institute for Respiratory Health, St. Joseph’s Hospital and the Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
Tracy
T
Tazzeo
Genevieve
G
Bates
Horia Nicolae
HN
Roman
Anne-Marie
AM
Lauzon
Mukta D
MD
Khasnis
Masumi
M
Eto
Luke J
LJ
Janssen
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D002110:Caffeine / Q000494:pharmacology*; D002417:Cattle; D057075:Enzyme Assays; D006801:Humans; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D058105:Polymerization / Q000187:drug effects*; D011189:Potassium Chloride / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology
D000199:Actins; D001704:Biopolymers; D007211:Indoles; D010726:Phosphodiesterase Inhibitors; D002110:Caffeine; D011189:Potassium Chloride; D013806:Theophylline; D009219:Myosin-Light-Chain Kinase; D010727:Phosphoric Diester Hydrolases; C000543:cyclopiazonic acid