Fast and robust multiple sequence alignment with phylogeny-aware gap placement.
BACKGROUND
ProGraphMSA is a state-of-the-art multiple sequence alignment tool which produces phylogenetically sensible gap patterns while maintaining robustness by allowing alternative splicings and errors in the branching pattern of the guide tree.


RESULTS
This is achieved by incorporating a graph-based sequence representation combined with the advantages of the phylogeny-aware gap placement algorithm of Prank. Further, we account for variations in the substitution pattern by implementing context-specific profiles as in CS-Blast and by estimating amino acid frequencies from input data.


CONCLUSIONS
ProGraphMSA shows good performance and competitive execution times in various benchmarks.
Department of Computer Science, ETH Zürich, Universitätstrasse, Switzerland. adam.szalkowski@inf.ethz.ch
Adam M
AM
Szalkowski
D000465:Algorithms; D017398:Alternative Splicing; D010802:Phylogeny; D016415:Sequence Alignment / Q000379:methods*; D020539:Sequence Analysis, Protein; D012984:Software