Sequence-based detection and breakpoint assembly of polymorphic inversions.
Inversion polymorphisms have occupied a privileged place in Drosophila genetic research since their discovery in the 1920s. Indeed, inversions seem to be nearly ubiquitous, and the majority of species that have been thoroughly surveyed have been found to be polymorphic for one or more chromosomal inversions. Despite enduring interest, however, inversions remain difficult to study because their effects are often cryptic, and few efficient assays have been developed. Even in Drosophila melanogaster, in which inversions can be reliably detected and have received considerable attention, the breakpoints of only three inversions have been characterized molecularly. Hence, inversion detection and assay design remain important unsolved problems. Here, we present a method for identification and local de novo assembly of inversion breakpoints using next-generation paired-end reads derived from D. melanogaster isofemale lines. PCR and cytological confirmations demonstrate that our method can reliably assemble inversion breakpoints, providing tools for future research on D. melanogaster inversions as well as a framework for detection and assay design of inversions and other chromosome aberrations in diverse taxa.
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA. russcd@gmail.com
Russell B
RB
Corbett-Detig
Charis
C
Cardeno
Charles H
CH
Langley
D000818:Animals; D001483:Base Sequence; D056905:Chromosome Breakpoints; D007446:Chromosome Inversion / Q000235:genetics*; D059006:Chromosomes, Insect / Q000235:genetics; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics; D005260:Female; D023281:Genomics; D005838:Genotype; D016133:Polymerase Chain Reaction