Cell surface molecules involved in infection mediated by lymphocytic choriomeningitis virus glycoprotein.
The glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV), the prototype arenavirus, is a promising envelope protein of lentiviral pseudotype vectors for gene therapy. The distribution of dystroglycan, a known receptor for LCMV, cannot explain the narrow tropism of LCMV-GP-pseudotypes. Here, we examined whether infection of LCMV-GP-pseudotypes was affected by the expression of four cell surface molecules-Axl and Tyro3 (from the TAM family) and DC-SIGN and LSECtin (from the C-type lectin family)-that are known receptors of Lassa virus, another arenavirus. All four molecules enhanced LCMV-GP-pseudotype infection of cells. These results help explain the tropism of LCMV-GP-pseudotypes and further our understanding of LCMV infection in animals.
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Masayuki
M
Shimojima
Yoshihiro
Y
Kawaoka
D001117:Arenaviridae Infections / Q000276:immunology* / Q000821:virology*; D015815:Cell Adhesion Molecules / Q000276:immunology; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D019169:Jurkat Cells; D037181:Lectins, C-Type / Q000276:immunology; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D020794:Receptor Protein-Tyrosine Kinases / Q000276:immunology; D011956:Receptors, Cell Surface / Q000276:immunology; D011991:Receptors, Virus / Q000276:immunology*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D037181:Lectins, C-Type; D020794:Receptor Protein-Tyrosine Kinases; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C505228:CLEC4G protein, human; C084944:TYRO3 protein, human