Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress.
Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil. The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity. In this study, utilizing (1)H NMR-based metabolite analysis combined with the physiological studies we assessed the effects of short-term water stress on overall growth, nitrogen fixation, ureide and proline dynamics, as well as metabolic changes in drought tolerant (NA5009RG) and sensitive (DM50048) genotypes of soybean in order to elucidate metabolite adjustments in relation to the physiological responses in the nitrogen-fixing plants towards water limitation. The results of our analysis demonstrated critical differences in physiological responses between these two genotypes, and identified the metabolic pathways that are affected by short-term water limitation in soybean plants. Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. silvente@ccg.unam.mx
Sonia
S
Silvente
Anatoly P
AP
Sobolev
Miguel
M
Lara
D000222:Adaptation, Physiological / Q000235:genetics / Q000502:physiology*; D000821:Animal Feed; D000818:Animals; D020369:Bradyrhizobium / Q000502:physiology; D018556:Crops, Agricultural / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D055864:Droughts; D005838:Genotype; D054884:Host-Pathogen Interactions; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009586:Nitrogen Fixation / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D025341:Principal Component Analysis; D011392:Proline / Q000378:metabolism; D053204:Root Nodules, Plant / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D013025:Glycine max / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*; D013559:Symbiosis; D014867:Water / Q000378:metabolism / Q000494:pharmacology*
D014867:Water; D011392:Proline