Maternal dietary folate and/or vitamin B12 restrictions alter body composition (adiposity) and lipid metabolism in Wistar rat offspring.
Maternal vitamin deficiencies are associated with low birth weight and increased perinatal morbidity and mortality. We hypothesize that maternal folate and/or vitamin B(12) restrictions alter body composition and fat metabolism in the offspring. Female weaning Wistar rats received ad libitum for 12 weeks a control diet (American Institute of Nutrition-76A) or the same with restriction of folate, vitamin B(12) or both (dual deficient) and, after confirming vitamin deficiency, were mated with control males. The pregnant/lactating mothers and their offspring received their respective diets throughout. Biochemical and body composition parameters were determined in mothers before mating and in offspring at 3, 6, 9 and 12 months of age. Vitamin restriction increased body weight and fat and altered lipid profile in female Wistar rats, albeit differences were significant with only B(12) restriction. Offspring born to vitamin-B(12)-restricted dams had lower birth weight, while offspring of all vitamin-restricted dams weighed higher at/from weaning. They had higher body fat (specially visceral fat) from 3 months and were dyslipidemic at 12 months, when they had high circulating and adipose tissue levels of tumor necrosis factor α, leptin and interleukin 6 and low levels of adiponectin and interleukin 1β. Vitamin-restricted offspring had higher activities of hepatic fatty acid synthase and acetyl-CoA-carboxylase and higher plasma cortisol levels. In conclusion, maternal and peri-/postnatal folate and/or vitamin B(12) restriction increased visceral adiposity (due to increased corticosteroid stress), altered lipid metabolism in rat offspring perhaps by modulating adipocyte function and may thus predispose them to high morbidity later.
Division of Endocrinology and Metabolism, National Institute of Nutrition, Indian Council of Medical Research (ICMR), Hyderabad-500 604, India.
Kalle Anand
KA
Kumar
Anumula
A
Lalitha
Dhandapani
D
Pavithra
Inagadapa J N
IJ
Padmavathi
Manisha
M
Ganeshan
Kalashikam Rajender
KR
Rao
Lagishetty
L
Venu
Nagala
N
Balakrishna
Nemani Hari
NH
Shanker
Singi Umakar
SU
Reddy
Giriraj Ratan
GR
Chandak
Shantanu
S
Sengupta
Manchala
M
Raghunath
D000273:Adipose Tissue / Q000187:drug effects; D000818:Animals; D000831:Animals, Newborn; D001724:Birth Weight; D001823:Body Composition / Q000187:drug effects*; D005260:Female; D005492:Folic Acid / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D014805:Vitamin B 12 / Q000494:pharmacology*; D014806:Vitamin B 12 Deficiency / Q000378:metabolism
D005492:Folic Acid; D014805:Vitamin B 12