Cultivable microbiota of Lithobates catesbeianus and advances in the selection of lactic acid bacteria as biological control agents in raniculture.
The cultivable microbiota of skin and cloaca of captive Lithobates catesbeianus includes microorganisms generally accepted as beneficial and potentially pathogenic bacteria. In order to select a group of potentially probiotic bacteria, 136 isolates were evaluated for their surface properties and production of antagonistic metabolites. Then, 11 lactic acid bacteria (LAB) strains were selected and identified as Lactobacillus plantarum, Lb. brevis, Pediococcus pentosaceus, Lactococcus lactis, L. garvieae and Enterococcus gallinarum. Studies of compatibility indicate that all the strains could be included in a multi-strain probiotic, with the exception of Ent. gallinarum CRL 1826 which inhibited LAB species through a bacteriocin-like metabolite. These results contribute to the design of a probiotic product to improve the sanitary status of bullfrogs in intensive culture systems, to avoid the use of antibiotics and thus to reduce production costs. It could also be an alternative to prevent infectious diseases during the ex situ breeding of amphibian species under threat of extinction.
Instituto Superior de Investigaciones Biológicas (INSIBIO-CONICET)-Instituto de Biología Dr. Francisco D. Barbieri, Facultad de Bioquímica, Química y Farmacia-Universidad Nacional de Tucumán, Chacabuco 461, CP T4000ILI, San Miguel de Tucumán, Argentina.
Gabriela Montel
GM
Mendoza
Sergio E
SE
Pasteris
Cesar E
CE
Ale
María C
MC
Otero
Marta I
MI
Bühler
María E Fátima
ME
Nader-Macías
D000822:Animal Husbandry; D000818:Animals; D003470:Culture Media; D007778:Lactobacillus / Q000235:genetics / Q000502:physiology*; D010572:Pest Control, Biological; D011898:Ranidae / Q000382:microbiology*
D003470:Culture Media