Fine mapping of the Bsr1 barley stripe mosaic virus resistance gene in the model grass Brachypodium distachyon.
The ND18 strain of Barley stripe mosaic virus (BSMV) infects several lines of Brachypodium distachyon, a recently developed model system for genomics research in cereals. Among the inbred lines tested, Bd3-1 is highly resistant at 20 to 25 °C, whereas Bd21 is susceptible and infection results in an intense mosaic phenotype accompanied by high levels of replicating virus. We generated an F(6:7) recombinant inbred line (RIL) population from a cross between Bd3-1 and Bd21 and used the RILs, and an F(2) population of a second Bd21 × Bd3-1 cross to evaluate the inheritance of resistance. The results indicate that resistance segregates as expected for a single dominant gene, which we have designated Barley stripe mosaic virus resistance 1 (Bsr1). We constructed a genetic linkage map of the RIL population using SNP markers to map this gene to within 705 Kb of the distal end of the top of chromosome 3. Additional CAPS and Indel markers were used to fine map Bsr1 to a 23 Kb interval containing five putative genes. Our study demonstrates the power of using RILs to rapidly map the genetic determinants of BSMV resistance in Brachypodium. Moreover, the RILs and their associated genetic map, when combined with the complete genomic sequence of Brachypodium, provide new resources for genetic analyses of many other traits.
State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing, China.
Yu
Y
Cui
Mi Yeon
MY
Lee
Naxin
N
Huo
Jennifer
J
Bragg
Lijie
L
Yan
Cheng
C
Yuan
Cui
C
Li
Sara J
SJ
Holditch
Jingzhong
J
Xie
Ming-Cheng
MC
Luo
Dawei
D
Li
Jialin
J
Yu
Joel
J
Martin
Wendy
W
Schackwitz
Yong Qiang
YQ
Gu
John P
JP
Vogel
Andrew O
AO
Jackson
Zhiyong
Z
Liu
David F
DF
Garvin
D058431:Brachypodium / Q000235:genetics* / Q000821:virology*; D019457:Chromosome Breakage; D020090:Chromosome Segregation / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D005843:Geography; D001467:Hordeum / Q000821:virology; D054643:INDEL Mutation / Q000235:genetics; D007178:Inbreeding; D008954:Models, Biological; D009029:Mosaic Viruses / Q000502:physiology*; D010641:Phenotype; D020161:Physical Chromosome Mapping / Q000379:methods*; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics; D013696:Temperature; D014421:Turkey
D005819:Genetic Markers