The gene transfection properties of a lipophosphoramidate derivative with two phytanyl chains.
Development of efficient and non-toxic gene delivery systems is among the most challenging requirements for successful gene therapy. Cationic lipophosphoramidates constitute a class of cationic lipids we have already shown to be efficient for in vivo gene transfer. Herein, we report the synthesis of a cationic lipophosphoramidate bearing two phytanyl chains (BSV18) as hydrophobic domain, and studied its gene transfection properties. In vitro, BSV18 exhibited a high transfection efficacy associated with a low cytotoxicity. (31)P NMR studies of various cationic lipophosphoramidates in water solution suggested that the phytanyl chains may favor the formation of an inverted hexagonal phase, a supramolecular arrangement which is presumed to enhance the endosomal escape and consequently increase the transfection efficiency. In vivo, systemic delivery of BSV18-based lipoplexes allowed a high efficiency of gene transfection into the mouse lung. With a view to clinical application, we evaluated not only the efficiency of lung transfection but also the eventual in vivo side-effects. Thus, in addition to monitoring the in vivo transfection efficiency by bioluminescent imaging and identifying by immunohistochemistry the cell types transfected, we also assessed in living animals the potential liver reaction as well as the inflammatory and immune responses induced by BSV18-mediated transfection. All those adverse effects were actually highly transient. Thus, taken together, these results indicate that lipophosphoramidates equipped with two phytanyl chains may have great potential for lung gene therapy, in particular for Cystic Fibrosis.
Unité INSERM 1078, IFR 148 ScInBioS, Université de Bretagne Occidentale, Université Européenne de Bretagne, 46 rue Félix Le Dantec, CS51819, 29218 Brest Cedex 02, France.
Mattias F
MF
Lindberg
Nathalie
N
Carmoy
Tony
T
Le Gall
Aurore
A
Fraix
Mathieu
M
Berchel
Christophe
C
Lorilleux
Hélène
H
Couthon-Gourvès
Pascale
P
Bellaud
Alain
A
Fautrel
Paul-Alain
PA
Jaffrès
Pierre
P
Lehn
Tristan
T
Montier
D000577:Amides / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D005260:Female; D018014:Gene Transfer Techniques / Q000009:adverse effects; D015316:Genetic Therapy; D006801:Humans; D007150:Immunohistochemistry; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D010756:Phosphoric Acids / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
D000577:Amides; D010756:Phosphoric Acids; C011067:phosphoramidic acid