Rhamnosyltransferase genes migA and wapR are regulated in a differential manner to modulate the quantities of core oligosaccharide glycoforms produced by Pseudomonas aeruginosa.
migA and wapR are rhamnosyltransferase genes involved in the biosynthesis of Pseudomonas aeruginosa lipopolysaccharide core oligosaccharide. Here, we show that preferential expression of migA and wapR correlated with the levels of uncapped and O polysaccharide-capped core, respectively. wapR is negatively regulated, while migA is positively regulated by RhlR/RhlI quorum sensing.
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
Dana
D
Kocíncová
Sarah L
SL
Ostler
Erin M
EM
Anderson
Joseph S
JS
Lam
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000378:metabolism; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D008025:Ligases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism*; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics*; D053038:Quorum Sensing; D014157:Transcription Factors / Q000378:metabolism
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; C417484:MigA protein, Pseudomonas aeruginosa; C086515:RhlR protein, Pseudomonas aeruginosa; D014157:Transcription Factors; D017853:Phosphotransferases (Alcohol Group Acceptor); C062877:rhamnulokinase; D008025:Ligases; C096955:RHLI protein, Pseudomonas aeruginosa