Characterization of a cfr-positive methicillin-resistant Staphylococcus epidermidis strain of the lineage ST22 implicated in a life-threatening human infection.
The cfr gene was identified in 3 methicillin-resistant Staphylococcus epidermidis (MRSE) isolates of lineage ST22 implicated in a fatal human infection. MRSE isolates displayed an indistinguishable pulsed-field gel electrophoresis profile and SCCmec type III, and showed a multiresistance phenotype. The presence of cfr, fexA, aac(6')-aph(2″), and dfrS1 genes was confirmed by polymerase chain reaction and sequencing. A mutation in 23S rRNA gene (C2534T) and amino acid changes and/or insertions in L3 and L4 proteins were detected. The cfr and fexA genes were located in a conjugative plasmid of approximately 45 kb and in the chromosome.
Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 51, 26006 Logroño, Spain.
Carmen
C
Lozano
Montserrat
M
Ruiz-García
Elena
E
Gómez-Sanz
Pilar
P
López-García
Gloria
G
Royo-García
Myriam
M
Zarazaga
Carmen
C
Torres
D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016000:Cluster Analysis; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D017809:Fatal Outcome; D005260:Female; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D008712:Methicillin / Q000494:pharmacology; D058889:Molecular Typing; D016254:Mutagenesis, Insertional; D010957:Plasmids; D017354:Point Mutation; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics; D013203:Staphylococcal Infections / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D012338:RNA, Ribosomal, 23S; D012269:Ribosomal Proteins; D008712:Methicillin