A conditionally replicating adenovirus carrying interleukin-24 sensitizes melanoma cells to radiotherapy via apoptosis.
Combinatorial therapy is the current trend of the development of novel cancer treatments due to the high heterogenous nature of solid tumors. In this study, we investigated the effects of the combined use of a conditionally replicating adenovirus carrying IL-24 (ZD55-IL-24) and radiotherapy on the proliferation and apoptosis of melanoma A375 cells in vitro and in vivo. Compared with either agent used alone, ZD55-IL-24 combined with radiotherapy significantly inhibited cell proliferation, accompanied with increased apoptosis. Radiotherapy did not affect the expression of IL-24 and E1A of ZD55-IL-24-treated cells, but increased the expression of Bax, promoted the activation of caspase-3, while decreasing Bcl-2 levels. Thus, this synergistic effect of ZD55-IL-24 in combination with radiotherapy provides a novel strategy for the development of melanoma therapies, and is a promising approach for further clinical development.
Department of Dermatology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
Guan
G
Jiang
Kai
K
Zhang
Ai-Jun
AJ
Jiang
Dan
D
Xu
Yong
Y
Xin
Zhi-Ping
ZP
Wei
Jun-Nian
JN
Zheng
Yan-Qun
YQ
Liu
D000256:Adenoviridae / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003131:Combined Modality Therapy; D015536:Down-Regulation / Q000187:drug effects / Q000528:radiation effects; D016503:Drug Delivery Systems; D006801:Humans; D007150:Immunohistochemistry; D007378:Interleukins / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D015854:Up-Regulation / Q000187:drug effects / Q000528:radiation effects; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D023041:Xenograft Model Antitumor Assays; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D000099180:Interleukin-24
D007378:Interleukins; D051028:bcl-2-Associated X Protein; D000099180:Interleukin-24