[Association of MMP14 gene polymorphisms and osteoporosis in Zhuang men from Baise region of Guangxi].
OBJECTIVE
To assess the association between membrane type 1 matrix metalloproteinase gene (MT1-MMP, MMP14) polymorphisms and osteoporosis in Zhuang men from Baise region of Guangxi.


METHODS
Genotypes of 5 loci (rs1003349, rs3751488, rs2269213, rs2236303 and rs743257) of MMP14 gene in 301 Zhuang men were determined with single base extension methods, and bone mineral density (BMD) at left calcaneus was evaluated with quantitative ultrasound with measured values of broadband ultrasonic attenuation (BUA). The subjects were divided according to BMD into osteoporosis group, osteopenia group and normal bone density group.


RESULTS
All selected loci were in Hardy-Weinberg equilibrium (P> 0.05). By multiple linear stepwise regression analysis, polymorphisms of the five loci were not associated with BUA. But a significant higher risk of osteoporosis was found in individuals with MMP14 rs1003349 GT genotype (vs. GG genotype; P<0.05) and rs2236303 CC and CT genotypes (vs. TT genotype; P<0.05). Genetic linkage between rs1003349 and rs2236303 was also discovered (D'= 0.839, r(2) = 0.458, P<0.01). Compared with the normal bone density group, the frequency of a G-T haplotype of rs1003349 and rs2236303 was significantly lower in the osteoporosis group (P<0.05). And the risk of osteoporosis for individuals with G-C and T-C haplotypes was 2.556 (95% CI: 1.029-6.349, P = 0.038) and 5.111 (95% CI: 1.341-19.485, P = 0.011) compared with G-T haplotype.


CONCLUSIONS
Polymorphisms of rs1003349 and rs2236303 loci of MMP14 gene are associated with the susceptibility of osteoporosis in Zhuang men in Guangxi. G-C and T-C haplotypes for loci rs1003349 and rs2236303 may increase the disease risk.
Department of Anatomy, Institute of Anthropology of Youjiang Medical University for Nationalities, Baise, Guangxi 533000, P. R. China.
Qing-hui
QH
Zhou
Xiu-feng
XF
Huang
Jin-hua
JH
Wang
Chao-wen
CW
Lin
Yuan-yuan
YY
Yang
Chang-sheng
CS
Huang
Lian-tao
LT
Wu
Yu-mei
YM
Wu
D000328:Adult; D000368:Aged; D015519:Bone Density / Q000235:genetics; D002681:China; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000235:genetics*; D008875:Middle Aged; D010024:Osteoporosis / Q000201:enzymology / Q000235:genetics*; D011110:Polymorphism, Genetic
C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14