Enhanced MHC class I and costimulatory molecules on B16F10 cells by Ganoderma lucidum polysaccharides.
OBJECTIVE
It is obvious that malignant cells evade from immune system in patients with manifest malignancy. Deficient major histocompatibility complex (MHC) class I and costimulatory molecules on malignant cells partially consist of evasion strategy since antigen bond MHC and costimulatory molecules provide two signals necessary for T cell activation. Therefore, enhancement of MHC-I and costimulatory molecules may favor restraint of the evasion. For this purpose, Ganoderma lucidum Polysaccharides (Gl-PS) was used on B16F10 melanoma cells in this study.


METHODS
Immunocytochemistry and flowcytometry were used to determine the H-2K(b) and H-2D(b) (two prominent MHC class I molecules in C57BL mouse) as well as B7-1 and B7-2 (two prominent costimulatory molecules) expression on B16F10 cells after incubation with Gl-PS, while messenger ribonucleic acid (mRNA) of these molecules was detected by reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
The H-2K(b) and H-2D(b), and B7-1 and B7-2 on B16F10 cells and mRNAs of these molecules were enhanced by Gl-PS, and more efficient antitumor cytotoxicity was induced by the Gl-PS treated cells.


CONCLUSIONS
The MHC class I molecules and costimulatory molecules may be enhanced by Gl-PS, and more efficient immune cell mediated cytotoxicity against these B16F10 cells may be induced, which may favor cancer therapy.
Department of Pharmacology, Peking University Health Science Center, School of Basic Medical Sciences, Beijing, China.
Li-Xin
LX
Sun
Zhi-Bin
ZB
Lin
Xin-Suo
XS
Duan
Jie
J
Lu
Zhi-Hua
ZH
Ge
Xue-Fei
XF
Li
Xue-Jun
XJ
Li
Min
M
Li
En-Hong
EH
Xing
You-Xin
YX
Song
Jing
J
Jia
Wei-Dong
WD
Li
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000627:therapeutic use*; D018122:B7-1 Antigen / Q000096:biosynthesis; D051940:B7-2 Antigen / Q000096:biosynthesis; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D005805:Genes, MHC Class I / Q000187:drug effects* / Q000235:genetics; D006183:H-2 Antigens / Q000096:biosynthesis; D008546:Melanoma, Experimental / Q000188:drug therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000737:chemistry / Q000627:therapeutic use*; D020940:Reishi / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D006183:H-2 Antigens; D011134:Polysaccharides