Influence of airflow intensity on phytase production by solid-state fermentation.
Phytase production by Aspergillus niger F3 by solid state fermentation (SSF) on citrus peel was evaluated at pilot scale under different aeration conditions. The best airflow intensity was 1 VkgM (Lair kg medium(-1) min(-1)), which allowed to produce 65 units of phytases per gram in dry basis (65 Ug(-1) d.b.) as it removed the metabolic heat generated by the microorganism, Agitation did not improve heat removal. Airflow intensity was considered as scale-up criterion. When the airflow intensity was maintained at 1 VkgM for SSF with 2 and 20 kg of medium, the kinetics parameters for biomass and enzyme concentration at the end of fermentation differed by less than 2. The air flow intensity was required to maintain the temperature and cool the SSF and to provide oxygen for microbial growth. Air flow intensity is a key a factor that must be considered when scale-up of SSF is attempted.
Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), P.O. Box 19011, 81.531-980 Curitiba, Brazil. danernesto72@yahoo.com
D E
DE
Rodríguez-Fernández
J A
JA
Rodríguez-León
J C
JC
de Carvalho
S G
SG
Karp
W
W
Sturm
J L
JL
Parada
C R
CR
Soccol
D010832:6-Phytase / Q000096:biosynthesis*; D000332:Aerobiosis; D000392:Air Movements; D001234:Aspergillus niger / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation / Q000502:physiology*; D006358:Hot Temperature; D007700:Kinetics
D010832:6-Phytase