Glicemical analysis of human blood serum using FT-Raman: a new approach.
BACKGROUND
Fourier transform Raman spectroscopy (FT-Raman) is a noninvasive diagnostic tool largely applied to the analysis of biological fluids and tissues.


METHODS
We examined the variation of carbohydrate concentration in human blood during 180 min relative to 29 spectra of patients split into four categories: hypoglycemic, healthy, threshold, and diabetic. The main bands monitored were placed in 960, 1030, 1091, 1128, and 1205 cm(-1). These bands were respectively attributed to C-O, C-C (stretching), C-O-H and C-O-C of carbohydrates. In this study, the Raman scattering signal of the all the blood samples was collected during 360 sec. The calculated correlation coefficient (R) between the concentration of carbohydrates and the Raman intensity was 0.923.


CONCLUSIONS
The obtained results are reasonable according to classical biochemical analysis. Our proposed FT-Raman-based method was shown to be suitable for the monitoring of carbohydrate concentration in human blood, and presented some advantages over classical biochemical methods, such as real-time analysis, required small sample volume, and was nondestructive, and the samples did not need any previous treatment.
Programa de Pós Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Brazil. j.saade@yahoo.com.br
Jamil
J
Saade
Josivandro do Nascimento
Jdo N
Silva
Patrícia M Albuquerque
PM
de Farias
Daniela Franco
DF
Lopes
Cássio Tristão
CT
dos Santos
Bruno de Araújo
Bde A
Farias
Kátia Calligaris
KC
Rodrigues
Airton Abrahão
AA
Martin
D001786:Blood Glucose / Q000032:analysis; D002241:Carbohydrates / Q000097:blood*; D003920:Diabetes Mellitus / Q000097:blood; D005583:Fourier Analysis; D006801:Humans; D007003:Hypoglycemia / Q000097:blood; D013059:Spectrum Analysis, Raman / Q000379:methods*
D001786:Blood Glucose; D002241:Carbohydrates