Development of a fluorescence immunochromatographic assay for the detection of zeta globin in the blood of (--(SEA)) α-thalassemia carriers.
Southeast Asian deletion (--(SEA)) α-thalassemia is an inherited monogenic disorder of human hemoglobin, and embryonic globin ζ (hemoglobin ζ, zeta globin chain or Hb zeta chain) has been shown to be a marker that can be used for the identification of carriers of the (--(SEA)) α-thalassemia deletion. In this work, a fluorescence immunochromatographic assay (FL-ICA) was established to detect the zeta globin chain in the hemolysates of carriers of the (--(SEA)) α-thalassemia deletion. This assay can be completed within 10min using a simple UV detector and does not suffer from interference from the red background color of the hemolysate. A total of 314 blood samples were tested by FL-ICA and ELISA. The results of these assays were confirmed by PCR, the standard technique for genetic disease testing. The sensitivity and specificity of this novel FL-ICA were 100% and 98.0%, respectively; the corresponding values for the ELISA performed simultaneously were 100% and 99.2%, respectively. In conclusion, a new FL-ICA-a simple, fast, convenient, low-cost method-was developed that may be useful in both high-throughput screening and individual detection of the (--(SEA)) α-thalassemia deletion in carriers. Additionally, this qualitative FL-ICA may enlighten the development of a new systems for analysis of other target molecules using whole-blood samples.
Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
Liyan
L
Wen
Ping
P
Zhu
Yanjun
Y
Liu
Qingjun
Q
Pan
Yanxia
Y
Qu
Xiangmin
X
Xu
Xueli
X
Li
Ning
N
Fu
D000328:Adult; D000911:Antibodies, Monoclonal; D001210:Asia, Southeastern; D001483:Base Sequence; D016022:Case-Control Studies; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D005820:Genetic Testing; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017384:Sequence Deletion; D017085:alpha-Thalassemia / Q000097:blood / Q000175:diagnosis* / Q000235:genetics*; D055543:zeta-Globins / Q000032:analysis* / Q000235:genetics
D000911:Antibodies, Monoclonal; D055543:zeta-Globins