Adipose tissue angiopoietin-like protein 4 messenger RNA changes with altered energy balance in lactating Holstein cows.
Negative energy balance at the onset of lactation is unfavorably associated with fitness traits in high-producing dairy cows. Angiopoietin-like protein 4 (ANGPTL4) is an adipokine that has been associated with the regulation of lipid metabolism through the inhibition of lipoprotein lipase activity and regulation of lipolysis. Expression of ANGPTL4 messenger RNA (mRNA) increases during early lactation, but its regulation with changing energy status is currently unknown. Accordingly, the objective of this study was to determine whether ANGPTL4 mRNA abundance is responsive to declining energy balance induced by the transition from pregnancy to lactation, feed restriction, and GH administration in lactating dairy cows. The mRNA abundance of leptin, adiponectin, and adiponectin receptor 2 were also measured to compare adipokine mRNA profiles during changes in energy metabolism. Repeated adipose tissue biopsies were taken from different cows during transition from late pregnancy to lactation (n = 26), feed restriction (n = 19), and GH administration (n = 20). As expected, milk yield increased with the onset of lactation and GH administration (P < 0.01) but declined during feed restriction. Energy balance declined in each experiment, resulting in negative energy balance at the onset of lactation and after feed restriction. Abundance of ANGPTL4 mRNA expression increased 2- to 6-fold with declining energy balance in each experiment. Leptin mRNA declined with feed restriction, and adiponectin mRNA decreased with the onset of lactation. The consistency and magnitude of the increase in ANGPTL4 mRNA across multiple models of altered energy balance identifies it as an adipokine that is uniquely responsive to changes in energy balance in the lactating dairy cow.
Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
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Koltes
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Spurlock
D052242:Adiponectin / Q000235:genetics; D000273:Adipose Tissue / Q000737:chemistry*; D042682:Angiopoietins / Q000235:genetics*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D005508:Food Deprivation / Q000502:physiology; D013006:Growth Hormone / Q000008:administration & dosage; D007774:Lactation / Q000378:metabolism*; D020738:Leptin / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis*; D054419:Receptors, Adiponectin / Q000235:genetics
D052242:Adiponectin; D042682:Angiopoietins; D020738:Leptin; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; D013006:Growth Hormone