Introduction to animal DNA barcoding protocols.
Procedures and protocols common to many DNA barcoding projects are summarized. Planning for any project should emphasize front-end procedures, especially the "genetic lockdown" of collected materials for downstream genetic procedures. Steps further into the DNA barcoding process chain, such as sequencing, data processing, and other back-end functions vary slightly, if at all, among projects and are presented elsewhere in the volume. Point-of-collection sample and tissue handling and data/metadata handling are stressed. Specific predictions of the future workflows and mechanics of DNA barcoding are difficult, so focus is on that which most or all future methods and technologies will surely share.
Laboratories of Analytical Biology, Smithsonian Institution, NMNH, Suitland, MD, USA. weigtl@si.edu
Lee A
LA
Weigt
Amy C
AC
Driskell
Andrea
A
Ormos
Christopher
C
Meyer
Allen
A
Collins
D000818:Animals; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D016133:Polymerase Chain Reaction
D004247:DNA