Osteogenic effect of fluvastatin combined with biodegradable gelatin-hydrogel.
The aim was to investigate the local osteogenic effect of fluvastatin incorporated into a biodegradable gelatin-hydrogel (GH) scaffold. The GH scaffolds were prepared through crosslinking by ultraviolet irradiation followed by freeze-drying. Two circular defects were surgically created on fifteen-week-old male rats calvaria. All defects of each rat were randomly filled with two of three treatments, specifically: fluvastatin incorporated into a GH disk (Flu-GH), distilled water incorporated into a GH disk, and no treatment. New bone formation was quantitatively analyzed after 7, 14, and 28 days using a micro-computed tomography (micro-CT) system, and histologically observed. Evaluation by micro-CT revealed a significant difference in new bone formation among the three kinds of defect. A highly osteogenic effect was observed in the Flu-GH group. The results showed that the fluvastatin incorporated into a biodegradable GH scaffold promoted osteogenesis in rat calvarial bone, indicating its potential for bone regeneration.
Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Mihama-ku, Chiba, Japan. kotanabe@tdc.ac.jp
Koji
K
Tanabe
Hideki
H
Nomoto
Naoto
N
Okumori
Tadashi
T
Miura
Masao
M
Yoshinari
D020341:Absorbable Implants; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D000077340:Fluvastatin; D020100:Hydrogels; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000601:surgery; D054457:Tissue Scaffolds; D014867:Water; D055114:X-Ray Microtomography
D005229:Fatty Acids, Monounsaturated; D020100:Hydrogels; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007211:Indoles; D014867:Water; D000077340:Fluvastatin