Overexpression of a maize sulfite oxidase gene in tobacco enhances tolerance to sulfite stress via sulfite oxidation and CAT-mediated H2O2 scavenging.
Sulfite oxidase (SO) plays an important role in sulfite metabolism. To date, the molecular mechanisms of sulfite metabolism in plants are largely unknown. Previously, a full-length cDNA of the putative sulfite oxidase gene from maize (ZmSO) was cloned, and its response to SO(2)/sulfite stress at the transcriptional level was characterized. In this study, the recombinant ZmSO protein was purified from E. coli. It exhibited sulfite-dependent activity and had strong affinity for the substrate sulfite. Over-expression (OE) of ZmSO in tobacco plants enhanced their tolerance to sulfite stress. The plants showed much less damage, less sulfite accumulation, but greater amounts of sulfate. This suggests that tolerance of transgenic plants to sulfite was enhanced by increasing SO expression levels. Interestingly, H(2)O(2) accumulation levels by histochemical detection and quantitative determination in the OE plants were much less than those in the wild-type upon sulfite stress. Furthermore, reductions of catalase levels detected in the OE lines were considerably less than in the wild-type plants. This indicates that SO may play an important role in protecting CAT from inhibition by excess sulfite. Collectively, these data demonstrate that transgenic tobacco plants over-expressing ZmSO enhance tolerance to excess sulfite through sulfite oxidation and catalase-mediated hydrogen peroxide scavenging. This is the first SO gene from monocots to be functionally characterized.
College of Life Science, Henan Agricultural University, Zhengzhou, People's Republic of China. xiazl08@163.com
Zongliang
Z
Xia
Kaile
K
Sun
Meiping
M
Wang
Ke
K
Wu
Hua
H
Zhang
Jianyu
J
Wu
D002374:Catalase / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D030821:Plants, Genetically Modified; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D050876:Sulfite Oxidase / Q000235:genetics*; D013447:Sulfites / Q000378:metabolism / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
D016166:Free Radical Scavengers; D013447:Sulfites; D006861:Hydrogen Peroxide; D002374:Catalase; D050876:Sulfite Oxidase