The dynamic changes of circulating OCN+ cells versus insulinlike growth factor-I during primary healing of orthognathic surgeries.
OBJECTIVE
The objective of this study was to determine the dynamic changes of circulating osteocalcin(+) (OCN(+)) cells and insulinlike growth factor-I (IGF-I) in peripheral blood during early primary repair of jaw bones in patients with orthognathic surgery.


METHODS
The expression of bone-related genes was detected by RT-PCR in circulating OCN(+) cells. The numbers of OCN(+) cells and serum level of IGF-I were determined by flow cytometry, immunocytochemical staining, and ELISA.


RESULTS
OCN(+) cells significantly increased in peripheral blood, and reached the peak at 1 to 2 weeks after surgery (P < .05). IGF-I in patients significantly decreased 1 week after surgery (P < .05), and then returned gradually to the normal level. There was no significant correlation between the number of circulating OCN(+) cells and the level of IGF-I (P > .05).


CONCLUSIONS
These findings suggested that circulating OCN(+) cells, at least in part, could be mobilized in response to bone injury, and contribute to bone repair in patients with orthognathic surgery.
Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.
Xi
X
Yang
Xiao-an
XA
Tao
Jian-qiang
JQ
Liang
Yi-jun
YJ
Huang
Xiao-ping
XP
Yang
D000328:Adult; D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics; D001854:Bone Marrow Cells / Q000166:cytology*; D001861:Bone Regeneration / Q000235:genetics* / Q000502:physiology; D019070:Cell Lineage; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D009504:Neutrophils / Q000166:cytology; D056948:Orthognathic Surgical Procedures; D010006:Osteoblasts / Q000166:cytology; D015675:Osteocalcin / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D010865:Pilot Projects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D055815:Young Adult
D024042:Collagen Type I; D015675:Osteocalcin; D007334:Insulin-Like Growth Factor I; D000469:Alkaline Phosphatase