Estradiol protects adipose tissue-derived stem cells against H(2)O(2)-induced toxicity.
Oxidative stress is associated with various pathophysiological processes, including cell survival, adhesion, apoptosis, and cancer. In the present study, we aimed to evaluate the effects of H(2)O(2)-induced toxicity on adipose tissue-derived stem cells (ADSCs) and whether 17β-estradiol (E(2)) has protective effects on these cells. ADSCs derived from adult Sprague-Dawley rats were pretreated with different doses of E(2) for 24 h and then exposed to 200 µM H(2)O(2) for 4 h. Incubation of ADSCs with H(2)O(2)-decreased cell viability in a concentration-dependent fashion (p < 0.0001), whereas pretreatment of these cells with E(2) significantly reversed toxicity (p < 0.05), inhibited apoptotic changes, and decreased lipid peroxidation (p < 0.0005). Our findings suggest that E(2) protects ADSCs from oxidative-induced cell death, and therefore, it may be used to improve the survival rate and regenerative capacity of stem cells.
Department of Physiology, Ege University School of Medicine, 35100 Izmir, Turkey. dilek.taskiran@ege.edu.tr
Dilek
D
Taskiran
Vedat
V
Evren
D053687:Adult Stem Cells / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020219:Chondrogenesis; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D050152:Intra-Abdominal Fat / Q000166:cytology*; D015227:Lipid Peroxidation / Q000187:drug effects; D010012:Osteogenesis; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D051381:Rats
D004967:Estrogens; D016877:Oxidants; D004958:Estradiol; D006861:Hydrogen Peroxide