Sequential deletion of all the polyketide synthase and nonribosomal peptide synthetase biosynthetic gene clusters and a 900-kb subtelomeric sequence of the linear chromosome of Streptomyces coelicolor.
Streptomyces coelicolor, with its 8 667 507-bp linear chromosome, is the genetically most studied Streptomyces species and is an excellent model for studying antibiotic production and cell differentiation. Here, we report construction of S. coelicolor derivatives containing sequential deletions of all the 10 polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) biosynthetic gene clusters and a 900-kb subtelomeric sequence (total c. 1.22 Mb, 14% of the genome). No obvious differences in growth rates and sporulation of the strains were found. An artificially circularized S. coelicolor genome with deletions of total c. 1.6 Mb segments (840-kb for the left and 761-kb for the right arm of the linear chromosome) was obtained. The actinorhodin biosynthetic gene cluster could be overexpressed in some of the constructed strains.
Key Laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai, China.
Min
M
Zhou
Xinyun
X
Jing
Pengfei
P
Xie
Weihua
W
Chen
Tao
T
Wang
Haiyang
H
Xia
Zhongjun
Z
Qin
D000880:Anthraquinones / Q000378:metabolism; D001483:Base Sequence; D002875:Chromosomes / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D055786:Gene Knockout Techniques; D015723:Gene Library; D016680:Genome, Bacterial; D005810:Multigene Family; D010453:Peptide Synthases / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D048630:Polyketide Synthases / Q000235:genetics*; D017384:Sequence Deletion; D013171:Spores, Bacterial; D048372:Streptomyces coelicolor / Q000201:enzymology / Q000235:genetics*; D000095622:Benzoisochromanequinones
D000880:Anthraquinones; D010453:Peptide Synthases; D048630:Polyketide Synthases; C013390:actinorhodin; D000095622:Benzoisochromanequinones