HPLC-ESI-MS and MS/MS structural characterization of multifucosylated N-glycoforms of the basic proline-rich protein IB-8a CON1+ in human saliva.
This study describes the characterization of the glycan moieties and the peptide backbone of six glycoforms of IB-8a CON1(+), a basic proline-rich protein present in human saliva. MS analyses on the intact glycoproteins before and after N-deglycosylation with PNGase F and high-resolution MS/MS sequencing by LTQ Orbitrap XL of peptides and glycopeptides from tryptic digests allowed the structural characterization of the glycan moieties and the polypeptide backbone, as well as to establish the glycosylation site at the asparagine residue at 98th position. Five of the glycoforms carry a biantennary N-linked glycan fucosylated in the innermost N-acetylglucosamine of the core and showing from zero to four additional fucoses in the antennal region. The sixth glycoform carries a monoantennary monofucosylated oligosaccharide. The glycoform cluster was detected on 28 of 71 adult saliva specimens. Level of fucosylation showed interindividual variability with the major relative abundance for the trifucosylated glycoform. Nonglycosylated IB-8a CON1(+) and the variant IB-8a CON1(-), lacking of the glycosylation site, have been also detected in human saliva.
Dipartimento di Scienze della Vita e dell'Ambiente, Università di Cagliari, Italy.
Tiziana
T
Cabras
Roberto
R
Boi
Elisabetta
E
Pisano
Federica
F
Iavarone
Chiara
C
Fanali
Sonia
S
Nemolato
Gavino
G
Faa
Massimo
M
Castagnola
Irene
I
Messana
D000293:Adolescent; D000328:Adult; D020816:Amino Acid Motifs; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006031:Glycosylation; D006801:Humans; D008297:Male; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012463:Saliva / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D055815:Young Adult
D011506:Proteins