Skin epithelial cells as possible substitutes for ameloblasts during tooth regeneration.
The disappearance of ameloblasts in erupted teeth hampers the implementation of tissue engineering-based tooth regeneration. We aimed at utilizing skin epithelial cells as the appropriate substitute for ameloblasts. The conversion potential of 1 day postnatal rat skin epithelial cells to ameloblasts was investigated under the induction of dental papillae mesenchymal cells (DPMCs). Induction strategies had been designed both in vitro and in vivo. Markers for ameloblasts had been detected in skin epithelial cells, which showed a columnar appearance with the nuclei located at one side, under indirect co-culture with DPMCs in vitro. An enamel-dentine-like and tooth germ-like structure was formed by recombining skin epithelial pieces or cells with DPMCs after 14 days of implantation in rat renal capsule. Immunohistochemistry and cell labelling analysis further demonstrated that the enamel-forming cells were skin epithelium-derived. These results indicated that the skin epithelium-derived cells from postnatal rats have the potential to convert to functional ameloblasts under effective induction.
Research and Development Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China; Institute of Stomatology, General Hospital of Chinese People's Liberation Army, Beijing, People's Republic of China.
Yihan
Y
Liu
Ming
M
Jiang
Wei
W
Hao
Wenjia
W
Liu
Liang
L
Tang
Hongchen
H
Liu
Yan
Y
Jin
D000565:Ameloblasts / Q000166:cytology*; D000818:Animals; D003771:Dental Papilla / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology* / Q000637:transplantation*; D007150:Immunohistochemistry; D020007:Implants, Experimental; D008648:Mesoderm / Q000166:cytology; D009930:Organic Chemicals / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D044968:Regenerative Medicine / Q000379:methods*; D012867:Skin / Q000166:cytology*; D013194:Staining and Labeling; D014070:Tooth / Q000166:cytology* / Q000502:physiology
D009930:Organic Chemicals; C070080:PKH 26