The complete mitochondrial genome of the wild silkworm moth, Actias selene.
The complete mitochondrial genome (mitogenome) of Actias selene (Lepidoptera: Saturniidae) was determined to be 15,236 bp, including 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes and a control region. The arrangement of 13 PCGs was similar to that of other sequenced lepidopterans. The AT skew of the mitogenome of A. selene was slightly negative, indicating a higher number of T compared to A nucleotides. The nucleotide composition of the mitogenome of A. selene was also biased toward A+T nucleotides (78.91%). All PCGs were initiated by ATN codons, except for the gene encoding cytochrome c oxidase subunit 1 (cox1), which may be initiated by the TTAG, as observed in other lepidopterans. Three genes, including cox1, cox2, and nad5, had incomplete stop codons consisting of just a T. With an exception for trnS1(AGN), all the other tRNA genes displayed a typical clover-leaf structure of mitochondrial tRNA. The A+T-rich region of the mitogenome of A. selene was 339 bp in length, and contains several features common to the Lepidopteras, including non-repetitive sequences, a conserved structure combining the motif ATAGA and an 18-bp poly-T stretch and a poly-A element upstream of trnM gene. Phylogenetic analysis showed that A. selene was close to Saturniidae.
Laboratory of Sericulture, College of Life Science, Anhui Agricultural University, 130 Changjiang West Road 230036, PR China.
Qiu-Ning
QN
Liu
Bao-Jian
BJ
Zhu
Li-Shang
LS
Dai
Guo-Qing
GQ
Wei
Chao-Liang
CL
Liu
D000818:Animals; D001483:Base Sequence; D018388:Codon, Terminator / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D049750:Genome, Insect / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics; D007915:Lepidoptera / Q000235:genetics; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics
D018388:Codon, Terminator; D019476:Insect Proteins; D012335:RNA, Ribosomal; D012343:RNA, Transfer; D003576:Electron Transport Complex IV