Molecular epidemiological survey of the Babesia gibsoni cytochrome b gene in western Japan.
In this study, we conducted a survey of the cytochrome b (cytb) gene of Babesia gibsoni (B. gibsoni) isolated from clinical cases to determine the prevalence of potential atovaquone (ATV)-resistant variants. Ninety-two blood samples were collected from naturally B. gibsoni infected dogs. The cytb nucleotide sequence was determined by direct sequencing. Twelve non-synonymous amino acid substitutions were identified in cytb. The principal ATV-resistant substitution, M121I, was detected in three cases. This survey determined that potentially ATV-resistant B. gibsoni strains are present in dogs in Japan.
Laboratory of Small Animal Internal Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima, Japan.
Masato
M
Sakuma
Kenta
K
Fukuda
Katsuyoshi
K
Takayama
Yukuharu
Y
Kobayashi
Takako
T
Shimokawa Miyama
Asuka
A
Setoguchi
Yasuyuki
Y
Endo
D000818:Animals; D053626:Atovaquone / Q000627:therapeutic use; D001403:Babesia / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D001404:Babesiosis / Q000188:drug therapy / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D045303:Cytochromes b / Q000235:genetics*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000188:drug therapy / Q000453:epidemiology* / Q000469:parasitology*; D004285:Dogs; D004791:Enzyme Inhibitors / Q000627:therapeutic use; D007564:Japan / Q000453:epidemiology; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics
D016054:DNA, Protozoan; D004791:Enzyme Inhibitors; D012337:RNA, Ribosomal, 18S; D045303:Cytochromes b; D053626:Atovaquone