Preoperative peripheral blood human telomerase reverse transcriptase mRNA concentration is not a prognostic factor for resection of hepatocellular carcinoma.
OBJECTIVE
Hepatocellular carcinoma (HCC) often recurs after complete surgical resection. Detection of markers of residual circulating cancer cells may predict postoperative HCC recurrence. Human telomerase reverse transcriptase (hTERT) mRNA may be a candidate tumor marker.


METHODS
We prospectively assessed the expression patterns and prognostic value of preoperative peripheral blood hTERT mRNA in patients with HCC undergoing hepatic resection (n=17) or liver transplantation (n=6). As controls, we assessed hTERT mRNA in patients with liver cirrhosis without HCC (n=6) and in living liver donors (n=4). Concentrations of hTERT mRNA were measured by real-time quantitative reverse transcription polymerase chain reaction (RTPCR).


RESULTS
No significant difference was observed in the levels of hTERT mRNA between the HCC and control groups. Only alpha-fetoprotein ≥400ng/mL was associated with greater expression levels of hTERT mRNA. At a median follow-up of 30 months, HCC recurred in 10 of 17 resected patients, but in none of the 6 liver transplant recipients. hTERT mRNA concentration was not associated with HCC recurrence after either resection or liver transplantation.


CONCLUSIONS
Peripheral blood hTERT mRNA concentration is not a likely marker for the diagnosis or prognosis of HCC, especially in patients undergoing resection. Owing to the small number of transplanted patients assessed, the clinical significance of hTERT mRNA concentration was not objectively verified, suggesting the need for a study in larger numbers of HCC patients undergoing liver transplantation.
Department of Surgery, University of Ulsan College of Medicine, Seoul, Korea.
Yeon-Dae
YD
Kim
Shin
S
Hwang
Young-Joo
YJ
Lee
Ki-Hun
KH
Kim
Chul-Soo
CS
Ahn
Kwang-Min
KM
Park
Deok-Bog
DB
Moon
Tae-Yong
TY
Ha
Gi-Won
GW
Song
Dong-Hwan
DH
Jung
Se-Ra
SR
Park
Hea-Nam
HN
Hong
Sung-Gyu
SG
Lee
D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000601:surgery; D005260:Female; D006498:Hepatectomy; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood / Q000601:surgery; D008113:Liver Neoplasms / Q000097:blood* / Q000601:surgery; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000097:blood*; D018365:Neoplasm, Residual; D009360:Neoplastic Cells, Circulating; D011237:Predictive Value of Tests; D057234:Preoperative Period; D012333:RNA, Messenger / Q000097:blood*; D018709:Statistics, Nonparametric; D019098:Telomerase / Q000097:blood*
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D019098:Telomerase