Dissolution and regeneration of collagen fibers using ionic liquid.
Native skin collagen fibers were successfully dissolved in the ionic liquid, 1-butyl-3-methylimidazolium chloride ([BMIM]Cl), and regenerated in different precipitators. The observation by polarized optical microscopy showed that the crystal structure of collagen fibers had been destroyed by [BMIM]Cl during the heating. Temperature-dependent FTIR was applied to detect the structural change of collagen/[BMIM]Cl during dissolving. The structure of regenerated collagen was characterized by FTIR and XRD. It showed that the triple helical structure of collagen had been partly destroyed during the dissolution and regeneration. The film forming ability and the thermostability of the regenerated collagen was highly dependent on the precipitating treatment. The possible mechanisms of dissolving of collagen in [BMIM]Cl and the regeneration in the precipitators have been proposed. The collagen/cellulose composite with different forms (film, fiber, gel) can be successfully prepared by using [BMIM]Cl as medium.
College of Materials Science and Engineering, Zhengzhou University, Henan 450052, China.
Zhuojun
Z
Meng
Xuejing
X
Zheng
Keyong
K
Tang
Jie
J
Liu
Zhi
Z
Ma
Qiaoling
Q
Zhao
D002482:Cellulose / Q000737:chemistry; D011232:Chemical Precipitation; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification*; D006358:Hot Temperature; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D012867:Skin / Q000737:chemistry; D012996:Solutions
D007093:Imidazoles; D052578:Ionic Liquids; D012996:Solutions; C502841:1-butyl-3-methylimidazolium chloride; D002482:Cellulose; D003094:Collagen