A simple, robust orthogonal background correction method for two-dimensional liquid chromatography.
Background correction is a very important step that must be performed before peak detection or any quantification procedure. When successful, this step greatly simplifies such procedures and enhances the accuracy of quantification. In the past, much effort has been invested to correct drifting baseline in one-dimensional chromatography. In fast online comprehensive two-dimensional liquid chromatography (LC×LC) coupled with a diode array detector (DAD), the change in the refractive index (RI) of the mobile phase in very fast gradients causes extremely serious baseline disturbances. The method reported here is based on the use of various existing baseline correction methods of one-dimensional (1D) liquid chromatography to correct the two-dimensional (2D) background in LC×LC. When such methods are applied orthogonally to the second dimension ((2)D), background correction is dramatically improved. The method gives an almost zero mean background level and it provides better background correction than does simple subtraction of a blank. Indeed, the method proposed does not require running a blank sample.
Department of Chemistry, Smith and Kolthoff Halls, University of Minnesota, 207 Pleasant Street S.E., Minneapolis, Minnesota 55455, USA.
Marcelo R
MR
Filgueira
Cecilia B
CB
Castells
Peter W
PW
Carr
D000465:Algorithms; D002853:Chromatography, Liquid; D012031:Refractometry