The relationship of maternal characteristics and circulating progesterone concentrations with reproductive outcome in the bottlenose dolphin (Tursiops truncatus) after artificial insemination, with and without ovulation induction, and natural breeding.
Bottlenose dolphins (Tursiops truncatus) undergoing natural breeding and artificial insemination (AI) were examined to characterize serum progesterone concentrations and determine relationships among age, parity, and reproductive outcome. Progesterone profiles of five cycle types (n = 119 total cycles from 54 animals) were characterized as follows: (i) conception and production of a live term calf (conceptive-term, n = 73); (ii) conception and abortion after Day 60 (conceptive-abortion, n = 12); (iii) unknown conception status with prolonged, elevated progesterone and absence of a fetus (conceptive-unknown, n = 14); (iv) conception failure with normal luteal phase progesterone concentrations (non-conceptive, n = 14, AI cycles only); and (v) conception failure with progesterone insufficiency occuring after spontaneous ovulation or owing to premature ovulation induction using GnRH (non-conceptive-PI, n = 6, AI cycles only). By Day 21 post-insemination (PI), progesterone concentrations were similar (P > 0.05) among conceptive-term, conceptive-abortion and conceptive-unknown, and higher (P < 0.05) for conceptive-term than non-conceptive and non-conceptive-PI cycles. Progesterone concentrations of known conceptive cycles peaked by Week 7 PI (P < 0.05) and remained elevated for the remainder of pregnancy (Weeks 8 up to 54, ≥ 5 days pre-partum). During midpregnancy (Days 121-240), conceptive-term cycles had higher (P > 0.05) progesterone concentrations than conceptive-abortion and unknown conception status cycles. Parity was not associated with reproductive outcome based on cycle type (P > 0.05). Age of females in conceptive-unknown (26.5 ± 10.1 yrs) and conceptive-abortion (22.1 ± 9.4 yrs) groups was higher (P < 0.05) than in conceptive-term (15.7 ± 7.2 yrs). The conceptive-unknown cycle type possibly represents undetected early embryonic loss occurring before Day 60 PI. Length of gestation using known conception dates was 376.1 ± 11.0 days and the range of this parameter (355-395 days) has implications for peri-parturient management procedures for the species.
SeaWorld and Busch Gardens Reproductive Research Center, SeaWorld Parks and Entertainment, 2595 Ingraham Street, San Diego, California 92109, USA. justine.obrien@seaworld.com
J K
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O'Brien
T R
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Robeck
D000034:Abortion, Veterinary; D000367:Age Factors; D000818:Animals; D050681:Bottle-Nosed Dolphin / Q000502:physiology*; D001947:Breeding; D005260:Female; D005306:Fertilization; D005865:Gestational Age; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage; D007315:Insemination, Artificial / Q000662:veterinary*; D010062:Ovulation Induction / Q000662:veterinary*; D010298:Parity; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000662:veterinary*; D011374:Progesterone / Q000097:blood*; D012621:Seasons; D014463:Ultrasonography / Q000662:veterinary
D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone