Chromosomal blaCTX-M-₁₅ associated with ISEcp1 in Proteus mirabilis and Morganella morganii isolated at the Military Hospital of Tunis, Tunisia.
This study investigated the genetic environment of bla(CTX-M) genes and associated resistance genes in seven Proteus mirabilis and six Morganella morganii extended spectrum β-lactamase (ESBL)-positive isolates. The isolates were recovered from hospitalized patients with respiratory or urinary tract infections at the Military Hospital of Tunis, Tunisia. Twenty-one of the 200 strains exhibited non-susceptibility to third generation cephalosporins and, among these strains, the double-disk synergy test confirmed the ESBL phenotype in 13 isolates. These ESBL producers were co-resistant to chloramphenicol, tetracycline and oflaxacin but remained susceptible to ertapenem (MIC<0.25 mg l(-1)). The presence and nature of bla(CTX-M-15), bla(CTX-M-8), bla(TEM-24), bla(TEM-1) and bla(TEM-2) genes was determined by PCR and sequencing. Chromosomal localization of the bla(CTX-M-15) gene was confirmed in all strains, with the exception of M. morganii isolate M-17991, by Southern-blot analysis performed either on chromosomal or plasmid DNA. M. morganii M-12012 and M. morganii M-6019 showed the same PFGE pattern, whereas the remaining CTX-M-15-producing isolates were unrelated. The presence of ISEcp1 was ascertained in CTX-M-15-producing isolates. A class 1 integron with different gene cassettes (dfrA1, orfC and aadB) was found in five P. mirabilis and six M. morganii isolates.
Laboratoire de Biochimie et Technobiologie, Universitè de Tunis, Tunisia.|Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi dell'Aquila, L'Aquila, Italy.
Sihem
S
Mahrouki
Laboratoire de Biochimie et Technobiologie, Universitè de Tunis, Tunisia.
Omrane
O
Belhadj
Laboratoire de Biochimie et Technobiologie, Universitè de Tunis, Tunisia.
Hela
H
Chihi
Laboratoire de Bacteriologie, Hospital Militaire de Tunis, Tunisia.
Ben Moussa
BM
Mohamed
Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi dell'Aquila, L'Aquila, Italy.
Giuseppe
G
Celenza
Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi dell'Aquila, L'Aquila, Italy.
Gianfranco
G
Amicosante
Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi dell'Aquila, L'Aquila, Italy.
Mariagrazia
M
Perilli
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology; D006772:Hospitals, Military / Q000706:statistics & numerical data*; D006801:Humans; D008826:Microbial Sensitivity Tests; D020613:Morganella morganii / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011513:Proteus mirabilis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D014416:Tunisia / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D047090:beta-Lactams; D001618:beta-Lactamases