Genome-wide location analysis reveals an important overlap between the targets of the yeast transcriptional regulators Rds2 and Adr1.
Upon glucose depletion, a massive reprogramming of gene expression occurs in the yeast Saccharomyces cerevisiae for the use of alternate carbon sources such as the nonfermentable compounds ethanol and glycerol. This process is mediated by the master kinase Snf1 that controls the activity of various targets including the transcriptional regulators Cat8, Sip4 and Adr1. We have recently identified Rds2 as an additional player in this pathway. Here, we have performed genome-wide location analysis of Rds2 in cells grown in the presence of glycerol. We show that Rds2 binds to promoters of genes involved in gluconeogenesis, the glyoxylate shunt, and the TCA cycle as well as some genes encoding mitochondrial components or some involved in the stress response. Interestingly, we also detected Rds2 at the promoters of SIP4, ADR1 and HAP4 which encodes the limiting subunit of the Hap2/3/4/5 complex, a regulator of respiration. Strikingly, we observed an important overlap between the targets of Rds2 and Adr1. Finally, we provide a model to account for the complex interplay among these transcriptional regulators.
Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tianthalay Road, Tha Kham, Bang Khuntian, Bangkok 10150, Thailand. nitnipa.soo@kmutt.ac.th
Nitnipa
N
Soontorngun
Sirilak
S
Baramee
Chalinee
C
Tangsombatvichit
Piyasuda
P
Thepnok
Supapon
S
Cheevadhanarak
François
F
Robert
Bernard
B
Turcotte
D002952:Citric Acid Cycle / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D026141:Drug Resistance, Multiple, Fungal / Q000235:genetics; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D055106:Genome-Wide Association Study; D005943:Gluconeogenesis / Q000235:genetics*; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology; D006019:Glycolysis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
C074682:ADR1 protein, S cerevisiae; D004268:DNA-Binding Proteins; C527549:Rds2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005990:Glycerol