The HIV-1 Nef protein interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA.
BACKGROUND
Human immunodeficiency virus type 1 (HIV-1) Nef-encoded protein plays key functions at almost all stages of the viral life cycle, but its role in translation is largely unknown.


METHODS
To determine the effect of Nef on translation we used an in vitro translation assay. The detection of Nef/RPS10 complexes and the presence of 18S rRNA and tRNAs in the complexes were performed by coimmunoprecipitation and RT-PCR assay.


RESULTS
We observed that the HIV-1 Nef protein specifically impaired translation in vitro. We observed the interaction of Nef with RPS10 by coimmunoprecipitation assay. In addition 18S rRNA and tRNAs were present in the Nef/RPS10 complexes.


CONCLUSIONS
Our results are consistent with a model in which the Nef protein by binding to two components of the 40S small ribosomal subunit, RPS10 and 18S rRNA, and to a lesser extent to tRNAs, could lead to decreased protein synthesis.
Department of Virology, University of Franche-Comte, UPRES EA 4266, IFR 133 INSERM, CHU Besancon, F-25030, Besançon, France.
Wasim
W
Abbas
Isabelle
I
Dichamp
Georges
G
Herbein
D000818:Animals; D002478:Cells, Cultured; D015497:HIV-1 / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D008954:Models, Biological; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012337:RNA, Ribosomal, 18S / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000378:metabolism*; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
D012337:RNA, Ribosomal, 18S; D012269:Ribosomal Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus; C514837:nef protein, Human immunodeficiency virus 1; C029537:ribosomal protein S10