Leveraging kinase inhibitors to develop small molecule tools for imaging kinases by fluorescence microscopy.
As the usage of fluorescence microscopy as a tool to study biological systems continues to grow, so does the need for additional tools that permit the selective detection of proteins of interest. Existing selective and well-characterized kinase inhibitors may be exploited to develop novel small molecule probes useful in imaging kinases by fluorescence microscopy.
Department of Chemistry, University of Massachusetts Boston, Boston, MA 02125, USA. wei2.zhang@umb.edu
Zijuan
Z
Zhang
Nicholas
N
Kwiatkowski
Hong
H
Zeng
Sang Min
SM
Lim
Nathanael S
NS
Gray
Wei
W
Zhang
Priscilla L
PL
Yang
D001896:Boron Compounds / Q000737:chemistry; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018385:Centrosome / Q000201:enzymology / Q000648:ultrastructure; D022004:Chromosome Structures / Q000201:enzymology / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000138:chemical synthesis; D047428:Protein Kinase Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011621:Pteridines / Q000737:chemistry* / Q000494:pharmacology; D000097869:Polo-Like Kinase 1
D001896:Boron Compounds; D018797:Cell Cycle Proteins; D005456:Fluorescent Dyes; D015335:Molecular Probes; D047428:Protein Kinase Inhibitors; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D011621:Pteridines; D000097869:Polo-Like Kinase 1; C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; C518477:BI 2536; C075840:TTK protein, human