Site-specific attachment of a protein to a carbon nanotube end without loss of protein function.
Establishing a nanobiohybrid device largely relies on the availability of various bioconjugation procedures which allow coupling of biomolecules and inorganic materials. Especially, site-specific coupling of a protein to nanomaterials is highly useful and significant, since it can avoid adversely affecting the protein's function. In this study, we demonstrated a covalent coupling of a protein of interest to the end of carbon nanotubes without affecting protein's function. A modified Staudinger-Bertozzi ligation was utilized to couple a carbon nanotube end with an azide group which is site-specifically incorporated into a protein of interest. We demonstrated that Ca(2+)-sensor protein, calmodulin, can be attached to the end of the nanotubes without affecting the ability to bind to the substrate in a calcium-dependent manner. This procedure can be applied not only to nanotubes, but also to other nanomaterials, and therefore provides a fundamental technique for well-controlled protein conjugation.
Graduate School of Biostudies, Kyoto University, Yoshida-konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan. yoshimura@lif.kyoto-u.ac.jp
Shige H
SH
Yoshimura
Shahbaz
S
Khan
Satoshi
S
Ohno
Takashi
T
Yokogawa
Kazuya
K
Nishikawa
Takamitsu
T
Hosoya
Hiroyuki
H
Maruyama
Yoshikazu
Y
Nakayama
Kunio
K
Takeyasu
D000596:Amino Acids / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002147:Calmodulin / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D037742:Nanotubes, Carbon / Q000737:chemistry*
D000596:Amino Acids; D001386:Azides; D002147:Calmodulin; D037742:Nanotubes, Carbon