Ecofriendly approach for detection of phenols in water using laccase from different fungi.
Laccase-initiated oxidative coupling reactions of phenol and its derivatives with 4-aminoantipyrene using air as an oxidant has been investigated. The oxidation reaction of phenols and 4-aminoantipyrene is getting a lot of attention due to environmental concerns. Oxidation of simple phenol and 4-aminoantipyrene as a benchmark reaction enabled us to rank the relative oxidation ability of various laccases. Among the laccases tested, laccase from Pycnoporus cinnabarinus successfully yielded 72% antipyrilquinoneimine dye. The present method can also be used to determine p-substituted phenols and chlorophenols. In this work, the influence of mediators on laccase activity has also been studied.
Department of Chemistry, University of Delhi, New Delhi, India. kidwai.chemistry@gmail.com
Mazaahir
M
Kidwai
Arti
A
Jain
Abha
A
Sharma
Ramesh
R
Chander Kuhad
D004784:Environmental Monitoring; D005658:Fungi / Q000201:enzymology*; D042845:Laccase / Q000378:metabolism*; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D014867:Water / Q000737:chemistry*
D010636:Phenols; D014867:Water; D042845:Laccase