Analgesic and antibutyrylcholinestrasic activities of the venom prepared from the Mediterranean jellyfish Pelagia noctiluca (Forsskal, 1775).
BACKGROUND
Toxins derived from jellyfishes have been exploited as a model for the development of new drug promising applications to treat neurodegenerative diseases. The present work is aimed to evaluate the acute toxicity of crude venom of Pelagia noctiluca and then to screen the analgesic and antibutyrylcholinestrasic (anti-BuChE) activities of the crude venom and its fractions.


METHODS
Sephadex G75 gel was used to separate crude venom of Pelagia noctiluca, which led to some fractions. In addition, in vivo analgesic and in vitro plasma antibutyrylcholinestrasic activities were carried out with Pelagia crude venom and its fractions respectively.


RESULTS
The crude venom and its fractions displayed analgesic and anti-BuChE activities at different doses without inducing acute toxicity. Fraction 2 possesses the highest analgesic and antibutyrylcholinestrasic properties. The crude venom and fraction 1 had shown to possess less significant inhibitory activity against analgesic and antibutyrylcholinestrasic models.


CONCLUSIONS
Based on this study, the crude venom of Pelagia noctiluca is found to be a useful tool for probing pharmacological activity. The purification and the determination of chemical structures of compounds of active fractions of the venom are under investigation.
Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry, Monastir university, Monastir, Tunisia.
Yosra
Y
Ayed
Afef
A
Dellai
Hedi
H
Ben Mansour
Hassen
H
Bacha
Salwa
S
Abid
D000700:Analgesics / Q000008:administration & dosage* / Q000302:isolation & purification; D000818:Animals; D002091:Butyrylcholinesterase / Q000378:metabolism*; D005591:Chemical Fractionation; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage* / Q000302:isolation & purification; D002850:Chromatography, Gel; D003064:Cnidarian Venoms / Q000008:administration & dosage* / Q000302:isolation & purification; D045424:Complex Mixtures / Q000008:administration & dosage / Q000302:isolation & purification; D003911:Dextrans; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005612:Freeze Drying; D008297:Male; D008522:Mediterranean Sea; D051379:Mice; D058894:Nematocyst / Q000737:chemistry; D037401:Scyphozoa / Q000737:chemistry*
D000700:Analgesics; D002800:Cholinesterase Inhibitors; D003064:Cnidarian Venoms; D045424:Complex Mixtures; D003911:Dextrans; C025614:sephadex; D002091:Butyrylcholinesterase