[Study on physiological and germination characteristics of Tulipa edulis seed].
OBJECTIVE
Current study was conducted to investigate the seed physiological characteristics of Tulipa edulis and improve germination rate.


METHODS
Anatomical characteristics was observed. Seed water absorption curve was tested by soaking method. Dynamic of embryo development and germination rate as well as germination index under different conditions were recorded. And the biological test of cabbage seed was used for detecting the germination inhibitors.


RESULTS
The embryo rate of newly matured seeds was about 10%, and there was no obstacle of water absorption on testa of T. edulis. The optimum method for embryo development was exposure to 300 mg x L(-1) gibberellin solution for 24 hours, and stratification at 25 degrees C for 70 days followed by stratification at 5 degrees C for 40 days. The germintion rate and germination index of dormancy-broken seeds under the dark environment at 10 degrees C and 15 degrees C were significantly higher than those under other conditions. Additionally, there were some germination inhibitory substances in dry seeds.


CONCLUSIONS
The seed of T. edulis can be classified as having complex morphophysiological dormancy, and the morphological embryo dormancy played a leading role. Warm and cold stratification resulted in a fast dormancy breaking effect, and a high germination rate more than 90% could be obtained under the optimum conditions.
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Zhengjun
Z
Wu
Zaibiao
Z
Zhu
Qiaosheng
Q
Guo
Hongliang
H
Ma
Hongjian
H
Xu
Yuanyuan
Y
Miao
D018525:Germination / Q000502:physiology*; D012639:Seeds / Q000502:physiology; D031447:Tulipa / Q000502:physiology*