[Experimental study on the ileum tissue proteins differentially expressed in Pi-qi deficiency syndrome rats].
OBJECTIVE
To identify the ileum tissue proteins differentially expressed in Pi-qi deficiency syndrome rats using proteomic approach.


METHODS
Thirty-seven rats were randomly divided into 3 groups, i. e., the normal control group (Group 1), the Pi-qi deficiency syndrome model group (Group 2), and the reserpine group (Group 3). The Pi-qi deficiency syndrome model was established using excessive exerting combined with irregular diet, and peritoneally injecting Reserpine Injection (1 mg/mL) respectively. The ileum tissues were separated after identified fuzzy method. The differentially expressed proteins were separated with two dimensional electrophoresis (2-DE), analyzed by Mass Spectrometry, and identified by MASCOT Software.


RESULTS
Three proteins were differentially expressed in Group 2 and nine proteins were differentially expressed in Group 3 (P < 0.05).


CONCLUSIONS
Pi-qi deficiency syndrome was closely related with decreased expression of albumin as well as increased expressions of trypsin and glucose-regulated protein 78.
RInstitute of Chinese Medical Literature, Liaoning University of Traditional Chinese Medicine, Shenyang 110847. swayling@sohu.com
Ling
L
Lu
Lian-Qun
LQ
Jia
Wei
W
Ma
D000418:Albumins / Q000378:metabolism; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism; D007082:Ileum / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D008516:Medicine, Chinese Traditional; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019413:Qi; D051381:Rats; D017207:Rats, Sprague-Dawley; D013577:Syndrome; D014357:Trypsin / Q000378:metabolism; D000091342:Endoplasmic Reticulum Chaperone BiP
D000418:Albumins; D004365:Drugs, Chinese Herbal; D006360:Heat-Shock Proteins; D020543:Proteome; D014357:Trypsin; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP