Synthesis, characterization, cell imaging and anti-tumor activity of multifunctional nanoparticles.
Most anticancer complexes are unable to differentiate between diseased and healthy cells, systemic toxicity and undesired side effects can result. In the current study, a PEG and RGD peptides functionalized fluorescent dye Rhodamine B isothiocyanate (RBITC) doped magnetic silica nanoparticle (MnFe(3)O(4)@SiO(2)-PEG-RGD), carrying a anticancer superparamagnetic Mn(II) complex, was synthesized and characterized using spectroscopic methods. The multifunctional nanoparticles (MnFe(3)O(4)@SiO(2)-PEG-RGD) can image HepG-2 cells and differentiate between HepG-2 and WRL-68 cells based on T(1) MR imaging technology. The in vitro fluorescence image and inhibition assay on the proliferation of HeLa cells indicate that MnFe(3)O(4)@SiO(2)-PEG-RGD nanoparticles can effectively reach the tumor site, be internalized by endocytosis and then retain in cancer cells due to the retention effect of nanoparticles. This study demonstrated that a PEG and RGD peptides functionalized silica nanoparticle was a good carrier for the anticancer complexes, and the anticancer complexes loaded multifunctional nanoparticles could be developed as special agents in monitoring therapy of cancer.
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. chenqy@ujs.edu.cn
Qiu-Yun
QY
Chen
Gen-Ping
GP
Tao
Ying-Qi
YQ
Liu
Xia
X
Yang
D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D048430:Cell Shape / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003952:Diagnostic Imaging; D005290:Ferric Compounds / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D012822:Silicon Dioxide / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
D000970:Antineoplastic Agents; D005290:Ferric Compounds; D009842:Oligopeptides; D012235:Rhodamines; C000499:ferric oxide; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; C047981:arginyl-glycyl-aspartic acid; C027755:rhodamine isothiocyanate