Cryopreservation of the bovine oocyte: current status and perspectives.
This review presents some of the most noticeable aspects related with the oocyte cryopreservation procedures, emphasizing their evolution in the bovine, which points towards the critical points determining the reduced survival rates of female gametes to freezing and vitrification. Factors such as the maturation status, the cytoskeleton and membrane sensitivity, the role of the cumulus cells, the impact of the cryoprotectants agents and the protocols utilized and the future of this tool have been extensively reviewed.
Centro de Biotecnología Animal-SERIDA, Gijón, Spain. mcdiez@serida.org
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Díez
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Muñoz
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Caamaño
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Gómez
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology; D015925:Cryopreservation / Q000662:veterinary*; D005260:Female; D009865:Oocytes / Q000166:cytology / Q000502:physiology*