Assessment of airborne exposure to endotoxin and pyrogenic active dust using electrostatic dustfall collectors (EDCs).
Passive airborne dust sampling using electrostatic dustfall collectors (EDCs) is one possibility especially for long sampling periods. In this study, EDCs were deposited in living rooms of private households and in social rooms of composting plants. The aim of the study was to determine whether endotoxin and pyrogenic activity are measurable using EDCs. In all extracts, endotoxin (via Limulus amebocyte lysate [LAL] assay) and pyrogenic activity (interleukin [IL]-1β release via whole blood assay) were detectable. In addition, the monocyte chemotactic protein (MCP-1; CCL-2) as a secondary proinflammatory marker was measured with whole blood assay. Endotoxin activity and proinflammatory/pyrogenic activity of EDC extracts from social rooms in composting plants were higher compared to extracts obtained from EDCs in private household rooms. A significant correlation between LAL assay and whole blood assay was detectable. In conclusion, EDC sampling is an applicable method to evaluate settled dust from airborne bioaerosols displaying a longer period of exposure. The extraction of EDC without Tween enables one to measure endotoxin as well as proinflammatory/pyrogenic activity using the same sample for parallel detection and more reliable characterization of the airborne bioaerosol contamination.
Institute for Prevention and Occupational Medicine of the German Social Accident Insurance (IPA), Institute of the Ruhr University Bochum, Bochum, Germany. liebers@ipadguv.de
Verena
V
Liebers
Vera
V
van Kampen
Jürgen
J
Bünger
Maria
M
Düser
Heike
H
Stubel
Thomas
T
Brüning
Monika
M
Raulf-Heimsoth
D018932:Chemokine CCL2 / Q000097:blood; D004391:Dust / Q000032:analysis*; D004731:Endotoxins / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D062665:Epidemiological Monitoring; D005374:Filtration; D005858:Germany / Q000453:epidemiology; D019570:Inhalation Exposure / Q000706:statistics & numerical data*; D053583:Interleukin-1beta / Q000097:blood; D008033:Limulus Test; D016273:Occupational Exposure / Q000706:statistics & numerical data; D012987:Soil; D012997:Solvents; D013048:Specimen Handling; D055672:Static Electricity
D018932:Chemokine CCL2; D004391:Dust; D004731:Endotoxins; D053583:Interleukin-1beta; D012987:Soil; D012997:Solvents