Covalent immobilization of ascorbate oxidase onto polycarbonate strip for L-ascorbic acid detection.
Herein, a simple and rapid method is described for detection of L-ascorbic acid by ascorbate oxidase immobilized onto polycarbonate strip pre-activated by 1-fluoro-2-nitro-4-azidobenzene in photochemical reaction. Covalent attachment of ascorbate oxidase was confirmed by XPS studies. The immobilized-ascorbate oxidase shows higher pH, thermal and storage stability in comparison to free enzyme.
Institute of Genomics and Integrative Biology-CSIR, Mall Road, Delhi 110007, India.
Dileep Kumar
DK
Kannoujia
Saroj
S
Kumar
Pradip
P
Nahar
D001204:Ascorbate Oxidase / Q000737:chemistry*; D001205:Ascorbic Acid / Q000032:analysis*; D001386:Azides / Q000378:metabolism; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D009578:Nitrobenzenes / Q000378:metabolism; D011076:Polycarboxylate Cement / Q000378:metabolism
C492759:1-fluoro-2-nitro-4-azidobenzene; D001386:Azides; D004800:Enzymes, Immobilized; D009578:Nitrobenzenes; D011076:Polycarboxylate Cement; C005506:polycarbonate; D001204:Ascorbate Oxidase; D001205:Ascorbic Acid