[Effect of hepatitis C virus core gene transfection on NFAT1 expression in human intrahepatic cholangiocarcinoma cells].
OBJECTIVE
To explore whether hepatitis C virus core protein (HCV C) regulates the expression of NFAT1 to participate in the progression and malignant biological behavior of intrahepatic cholangiocarcinoma cells.


METHODS
The recombinant plasmid pEGFP-N(3)-HCV C and the empty vector pEGFP-N(3) were cotransfected with enhanced green fluorescent protein (EGFP) into RBE cells using liposome. Real-time PCR and Western blotting were used to examine the expression of NFAT1 mRNA and protein in the transfected RBE cells. MTT assay was used to evaluate the changes in the cell proliferation, and the cell cycle changes were analyzed by flow cytometry.


RESULTS
HCV C transfection significantly enhanced the expressions of NFAT1 mRNA and protein in RBE cells (P<0.05) and promoted the progression of cell cycle into G(2)/M phase to accelerate the cell proliferation.


CONCLUSIONS
Transfection with HCV C gene up-regulates NFAT1 expression and promotes the cell cycle progression and proliferation of intrahepatic cholangiocarcinoma cells, suggesting the involvement of HCV C in the progression of intrahepatic cholangiocarcinoma.
Department of Oncology, Sun Yat-sen University, Guangzhou, China. idea39@126.com
Qiaofang
Q
Liao
Zhihua
Z
Li
Rufu
R
Chen
Ning
N
Guo
Bing
B
Zeng
Di
D
Cheng
Liping
L
Zheng
D001650:Bile Duct Neoplasms / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018281:Cholangiocarcinoma / Q000473:pathology*; D015870:Gene Expression; D006801:Humans; D050778:NFATC Transcription Factors / Q000235:genetics*; D010957:Plasmids; D014162:Transfection; D014758:Viral Core Proteins / Q000235:genetics*
D050778:NFATC Transcription Factors; C494006:NFATC2 protein, human; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus