Biodesulfurization of dibenzothiophene by Gordonia sp. AHV-01 and optimization by using of response surface design procedure.
A novel desulfurizing bacterium has been isolated from oil-contaminated soils in Khuzestan. The ability for dibenzothiophene desulfurization and its biochemical pathway were investigated. The bacterium was identified as Gordonia sp. AHV-01 (Genbank Accession No HQ607780) by 16S rRNA gene sequencing. HPLC results and Gibb's assay were shown that dibenzothiophene desulfurized via 4S-pathway Maximum growth (0.426 g dry cells/L) and produced 2-hydroxybiphenyl (63.1 microM) were observed at 120 h of cultivation. By using of response surface design procedure the optimization of pH, temperature and rotary shaker round on the desulfurization reaction of isolate AHV-01 were performed. The optimum conditions were determined at pH of 7.0, temperature of 30 degrees C and rotary shaker round of 180 rpm. At these conditions, the dibenzothiophene desulfurization activity was increased and maximum 2-hydroxybiphenyl production was detected 70.29 microM at 96 h. According to these results, Isolate AHV-01 was capable to desulfurize dibenzothiophene via 4S-pathway and likely it can be useful to reduce organic sulfur contents of crude oil.
Department of Microbiology, Islamic Azad University, Masjedsoleyman Branch, Iran. a_aminsefat@yahoo.com
Ayeh
A
Aminsefat
Behnam
B
Rasekh
Mohammad Roayaei
MR
Ardakani
D033601:Gordonia Bacterium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007492:Iran; D010578:Petroleum / Q000378:metabolism / Q000382:microbiology; D059392:Petroleum Pollution; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods; D012988:Soil Microbiology; D013455:Sulfur / Q000378:metabolism; D013876:Thiophenes / Q000378:metabolism*
D010578:Petroleum; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013876:Thiophenes; D013455:Sulfur; C016366:dibenzothiophene