WhiB2/Rv3260c, a cell division-associated protein of Mycobacterium tuberculosis H37Rv, has properties of a chaperone.
whiB-like genes have been found in all actinomycetes sequenced so far. The amino-acid sequences of WhiB proteins of Mycobacterium tuberculosis H37Rv are highly conserved and participate in several cellular functions. Unlike other WhiB proteins of M. tuberculosis that have properties of protein disulfide reductases, WhiB2 showed properties like a chaperone as it suppressed the aggregation of several model substrates (e.g. citrate synthase, rhodanese and luciferase). Suppression of aggregation of the model substrates did not require ATP. Four cysteine residues of WhiB2 form two intramolecular disulfide bonds; however, chaperone function was unaffected by the redox state of the cysteines. WhiB2 also restored the activity of chemically denatured citrate synthase and did not require either ATP or a co-chaperone for refolding. The results indicate that WhiB2, which has been shown to be associated with cell division in mycobacteria and streptomyces, has evolved independently of other WhiBs, although it retains basic properties of this group of proteins. This is the first report to show that a WhiB protein has chaperone-like function; therefore, this report will have major implications in attempts to understand the role of WhiB proteins in mycobacteria, particularly in cell division.
Institute of Microbial Technology/CSIR, Chandigarh, India.
Monica
M
Konar
Md Suhail
MS
Alam
Chandni
C
Arora
Pushpa
P
Agrawal
D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D011232:Chemical Precipitation; D002950:Citrate (si)-Synthase / Q000737:chemistry / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D007328:Insulin / Q000737:chemistry / Q000378:metabolism; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000502:physiology*; D010084:Oxidation-Reduction; D011489:Protein Denaturation; D012694:Serine / Q000737:chemistry
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007328:Insulin; D018832:Molecular Chaperones; D012694:Serine; D000255:Adenosine Triphosphate; D002950:Citrate (si)-Synthase; D003545:Cysteine