Self-assembled magnetic bead biosensor for measuring bacterial growth and antimicrobial susceptibility testing.
Bacterial antibiotic resistance is one of the major concerns of modern healthcare worldwide, and the development of rapid, growth-based, antimicrobial susceptibility tests is key for addressing it. The cover image shows a self-assembled asynchronous magnetic bead rotation (AMBR) biosensor developed for rapid detection of bacterial growth. Using the biosensors, the minimum inhibitory concentration of a clinical E. coli isolate can be measured within two hours, where currently tests take 6-24 hours. A 16-well prototype is also constructed for simple and robust observation of the self-assembled AMBR biosensors.
University of Michigan, Department of Chemistry, 930 N. University, Ann Arbor, MI 48109-1055, USA. pkkinn@umich.edu
Paivo
P
Kinnunen
Brandon H
BH
McNaughton
Theodore
T
Albertson
Irene
I
Sinn
Sima
S
Mofakham
Remy
R
Elbez
Duane W
DW
Newton
Alan
A
Hunt
Raoul
R
Kopelman
D000890:Anti-Infective Agents / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D008280:Magnetics / Q000295:instrumentation*; D008826:Microbial Sensitivity Tests / Q000295:instrumentation* / Q000379:methods*; D008863:Microspheres; D012399:Rotation
D000890:Anti-Infective Agents