Safe deprotection strategy for the tert-butyldimethylsilyl (TBS) group during RNA synthesis.
This unit describes two protocols for the deprotection of 2'-O-TBS groups in oligoribonucleotides under mild conditions. Desilylation using ammonium fluoride is applicable to fully protected "RNA only" substrates and desilylation using potassium fluoride is applicable to "mixed RNA/non-RNA" substrates. Characterization of products is accomplished using LC/MS, RP HPLC and SAX HPLC.
Department of Process Research, Merck Research Laboratories, Rahway, New Jersey, USA.
Daniel
D
Zewge
D064751:Ammonium Compounds; D002851:Chromatography, High Pressure Liquid; D005459:Fluorides / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000138:chemical synthesis*; D017646:Organosilicon Compounds / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis*
D064751:Ammonium Compounds; D015345:Oligonucleotide Probes; D017646:Organosilicon Compounds; D000644:Quaternary Ammonium Compounds; C009660:t-butyldimethylsilyl compounds; C024822:ammonium fluoride; D012313:RNA; D005459:Fluorides