In vitro explant culture and related protocols for the study of mouse retinal development.
The mouse retina is composed of many cell types and subtypes with distinct morphology and function; how these cells are differentiated from the multipotent progenitors is still largely unknown. Retinal in vitro explant culture has proven to be a useful tool to study the molecular and cellular mechanisms underlying retinal development. Here, we provide detailed descriptions about how to prepare retroviruses, dissect retinal cups, perform in vitro explant culture, and collect explant samples.
Department of Pediatrics, Center for Advanced Biotechnology and Medicine, Graduate Program in Molecular Genetics, Microbiology and Immunology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ, USA.
Kangxin
K
Jin
Mengqing
M
Xiang
D000818:Animals; D018274:Electroporation; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D007150:Immunohistochemistry; D051379:Mice; D012160:Retina / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics; D046509:Tissue Culture Techniques