Gaetbulibacter lutimaris sp. nov., isolated from a tidal flat sediment.
A Gram-stain-negative, aerobic, non-flagellated, non-gliding, rod-shaped bacterial strain, D1-y4(T), was isolated from a tidal flat sediment of the South Sea in South Korea and subjected to a polyphasic study. Strain D1-y4(T) grew optimally at 25 °C, at pH 7.0-7.5 and in the presence of 2-3 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain D1-y4(T) belonged to the genus Gaetbulibacter, joining the type strain of Gaetbulibacter marinus, with which it exhibited 97.8 % similarity. Sequence similarities to Gaetbulibacter saemankumensis SMK-12(T) and Gaetbulibacter aestuarii KYW382(T) were 96.5 and 96.2 %, respectively. Strain D1-y4(T) contained MK-6 as the predominant menaquionone and iso-C15 : 0, iso-C15 : 1 G and anteiso-C15 : 0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain D1-y4(T) was 34.6 mol% and its mean DNA-DNA relatedness value with G. marinus KCTC 23046(T) was 7 %. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain D1-y4(T) is distinguishable from the three recognized Gaetbulibacter species. On the basis of the data presented here, strain D1-y4(T) is considered to represent a novel species of the genus Gaetbulibacter, for which the name Gaetbulibacter lutimaris sp. nov. is proposed. The type strain is D1-y4(T) ( = KCTC 23716(T)  = CCUG 61504(T)).
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea.
Jung-Hoon
JH
Yoon
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 306-809, Republic of Korea.
Soo-Young
SY
Lee
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 306-809, Republic of Korea.
Tae-Kwang
TK
Oh
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6