Bacteriophage control of vancomycin-resistant enterococci in cattle compost.
OBJECTIVE
To isolate bacteriophage that infects vancomycin-resistant enterococci (VRE) and to investigate the ability of this phage to diminish VRE number in vitro and in experimentally VRE-inoculated compost.


RESULTS
We sampled 106 solid or water samples, including 101 bovine faecal samples; lytic phage named Vrep-5 was isolated from one bovine faecal sample by plaque assay using the clinical VRE isolate FN1 (Enterococcus faecium). Vrep-5 generated clear plaques 1 mm in diameter and exhibited characteristics of the family Myoviridae A1, with a spherical head (122 ± 16 nm) and a contractile tail (152 ± 17 nm long). Vrep-5 lysed other bacterial strains, including Enterococcus faecalis. Inoculation of vrep-5 into 0.5 g unsterilized compost experimentally inoculated with FN1 at the multiplicity of infection of 1500 (8.8 × 10(4) CFU g(-1) VRE and 1.3 × 10(8) PFU g(-1) vrep-5) led to a decrease of >3 log(10) in VRE abundance compared with the untreated control after 24 h of incubation.


CONCLUSIONS
The data show that bacteriophage vrep-5 is effective in the rapid reduction in VRE colonization in compost.


CONCLUSIONS
The present study gives valuable new knowledge in the fight against VRE in the animal production.
Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, Osaki, Miyagi, Japan.
K
K
Otawa
Y
Y
Hirakata
M
M
Kaku
Y
Y
Nakai
D000818:Animals; D001435:Bacteriophages / Q000302:isolation & purification / Q000472:pathogenicity*; D002417:Cattle; D013293:Enterococcus faecalis / Q000302:isolation & purification / Q000821:virology*; D016984:Enterococcus faecium / Q000302:isolation & purification / Q000821:virology*; D008372:Manure / Q000382:microbiology*; D012988:Soil Microbiology; D020713:Vancomycin Resistance; D010948:Viral Plaque Assay
D008372:Manure