Determination of ortho-phthalaldehyde in water by high performance liquid chromatography and gas chromatography-mass spectrometry after hydrazine derivatization.
A simple high performance liquid chromatographic (HPLC) method and a highly sensitive gas chromatography mass spectrometric (GC-MS) method have been established for the determination of ortho-phthalaldehyde (OPA) in water. These methods are based on the derivatization of OPA with hydrazine in water. The following optimum reaction conditions were established: reagent dosage, 20 mg/mL of hydrazine; pH 2; reaction for 20 min at 70 °C. The organic derivative was detected directly by HPLC or after the extraction with methylene chloride/concentration by GC-MS. The limit of detection of OPA in water was 4.0 and 0.3 μg/L by HPLC and GC-MS, respectively. The calibration curve showed good linearity with r² = 0.9993 and r² = 0.9994 by HPLC and GC-MS, respectively, the accuracy was in a range of 95-105%, and the precision of the assay was less than 13% in water. The HPLC method was simple and reproducible enough to permit the OPA content analysis in the disinfectant products, and the GC-MS method is sensitive enough to permit reliable analysis of OPA to the μg/L level in environmental water.
Department of Environmental Science, Kongju National University, Kongju 314-701, South Korea.
Jin-Aa
JA
Oh
Ho-Sang
HS
Shin
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006834:Hydrazines / Q000737:chemistry; D057230:Limit of Detection; D012044:Regression Analysis; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D009764:o-Phthalaldehyde / Q000032:analysis*
D006834:Hydrazines; D014874:Water Pollutants, Chemical; C029424:hydrazine; D009764:o-Phthalaldehyde