Utility of saliva and hair follicles in donor selection for hematopoietic stem cell transplantation and chimerism monitoring.
Selection of an HLA identical donor is a critical pre-requisite for successful hematopoietic stem cell transplantation (HSCT). Most transplant centers utilize blood as the most common source of DNA for HLA testing. However, obtaining blood through phlebotomy is often challenging in patients with conditions like severe leucopenia or hemophilia, pediatric and elderly patients. We have used a simple in-house protocol and shown that HLA genotypes obtained on DNA extracted from saliva or hair are concordant with blood and hence can be used for selection of donors for HSCT or organ transplantation. Similarly, for post-HSCT chimerism monitoring, non-availability of pre-transplant DNA samples poses a major limitation of reference STR fingerprints. This study shows that DNA obtained post-HSCT from hair follicles can be used to generate pre-transplant patient specific fingerprints while the STR profiles obtained in saliva samples cannot as these display a mixed state of chimerism.
Department of Transplant Immunology and Immunogenetics, India Institute of Medical Sciences; New Delhi, India. gurvinder@hotmail.com
Gurvinder
G
Kaur
Neeraj
N
Kumar
Ramya
R
Nandakumar
Chowphi C
CC
Rapthap
Gaurav
G
Sharma
Shekhar
S
Neolia
Heena
H
Kumra
Prateek
P
Mahalwar
Abhinav
A
Garg
Sunil
S
Kumar
Jasmeet
J
Kaur
Mrinali
M
Hakim
Lalit
L
Kumar
Narinder K
NK
Mehra
D000293:Adolescent; D000328:Adult; D000483:Alleles; D046528:Chimerism; D004247:DNA / Q000235:genetics; D016172:DNA Fingerprinting; D046148:Donor Selection / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D005260:Female; D015234:HLA-A Antigens / Q000235:genetics; D018859:Hair Follicle / Q000378:metabolism*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000378:metabolism*; D020080:Tandem Repeat Sequences / Q000235:genetics; D055815:Young Adult
D015234:HLA-A Antigens; D004247:DNA