Fucose-specific conjugation of hydrazide derivatives to a vascular-targeting monoclonal antibody in IgG format.
We describe a method that enables specific and efficient conjugation of hydrazide-moieties to an IgG targeting the tumor neovasculature. The resulting chemically defined, homogeneous hydrazone-linked IgG conjugates remain immunoreactive and have a half-life of approximately 18 hours at physiological pH and temperature suitable for localized delivery of toxic drugs.
Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETH Zürich), Wolfgang-Pauli Str. 10, 8093 Zürich, Switzerland.
Kathrin
K
Zuberbühler
Giulio
G
Casi
Gonçalo J L
GJ
Bernardes
Dario
D
Neri
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D005643:Fucose / Q000737:chemistry* / Q000276:immunology; D006834:Hydrazines / Q000737:chemistry* / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology; D013696:Temperature
D000911:Antibodies, Monoclonal; D006834:Hydrazines; D007074:Immunoglobulin G; D009844:Oligosaccharides; D005643:Fucose