Cyclic caged morpholinos: conformationally gated probes of embryonic gene function.
Feeling a bit cagey: morpholino-based antisense reagents have been caged through oligonucleotide cyclization, enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.
Department of Chemical and Systems Biology, Stanford University School of Medicine, CA 94305, USA.
Sayumi
S
Yamazoe
Ilya A
IA
Shestopalov
Elayne
E
Provost
Steven D
SD
Leach
James K
JK
Chen
D000818:Animals; D002454:Cell Differentiation; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000166:cytology / Q000378:metabolism*; D060172:Morpholinos / Q000494:pharmacology*; D009841:Oligonucleotides / Q000494:pharmacology*; D038081:Organogenesis; D010179:Pancreas / Q000196:embryology / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism
D060172:Morpholinos; D009841:Oligonucleotides; D049452:Green Fluorescent Proteins