Inhibitory effects of microRNA-34a on cell migration and invasion of invasive urothelial bladder carcinoma by targeting Notch1.
MicroRNAs (miRNAs or miRs) are a class of short, non-coding RNAs that participate in various oncological processes. This study aims to explore the roles of microRNA-34a (miR-34a) in invasive urothelial bladder carcinoma. miR-34a was transfected into bladder cancer cell lines 253J and J82. The miR-34a expression levels in tissues and cells were detected by using qRT-PCR. The Notch1 expression was detected by qRT-PCR and Western blotting. Cell migratory and invasive abilities were measured by Transwell chamber assay. Bioinformatics and luciferase assay were performed to predict and analyze the binding sites between miRNA-34a and Notch1. It was found that there was aberrant expression of miR-34a in bladder cancer tissues. Moreover, we revealed that ectopic expression of miR-34a suppressed cell migration and invasion, while forced expression of Notch1 increased cell migratory and invasive abilities. Finally, we observed that miR-34a transfection significantly down-regulated luciferase activity and reduced the mRNA and protein levels of Notch1. Our study concluded that microRNA-34a antagonizes Notch1 and inhibits cell migration and invasion of bladder cancer cells, which indicates the tumor-suppressive function of microRNA-34a in bladder cancer.
Department of Urology, Chinese PLA General Hospital, Beijing, 100853, China.|Nankai University, Tianjin, 300071, China.
Chao
C
Zhang
Department of Urology, Air Force General Hospital of PLA, Beijing, 1000853, China.
Zhiyong
Z
Yao
Department of Urology, Chinese PLA General Hospital, Beijing, 100853, China.
Mingyang
M
Zhu
Department of Urology, Chinese PLA General Hospital, Beijing, 100853, China.
Xin
X
Ma
Department of Urology, Chinese PLA General Hospital, Beijing, 100853, China.
Taoping
T
Shi
Department of Urology, Chinese PLA General Hospital, Beijing, 100853, China.
Hongzhao
H
Li
Department of Urology, Chinese PLA General Hospital, Beijing, 100853, China.
Baojun
B
Wang
Department of Endocrinology, Chinese PLA General Hospital, Beijing, 100853, China.
Jinzhi
J
Ouyang
Department of Urology, Chinese PLA General Hospital, Beijing, 100853, China. xzhang@tjh.tjmu.edu.cn.
Xu
X
Zhang
D000328:Adult; D000368:Aged; D002465:Cell Movement / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D018390:Gene Targeting; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D051881:Receptor, Notch1 / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000473:pathology* / Q000503:physiopathology*
C522425:MIRN34 microRNA, human; D035683:MicroRNAs; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1