[Characteristics of Bordetelia pertussis strains isolated from pertussis patients in Moscow by using multilocus sequencing].
OBJECTIVE
Genotyping of B. pertussis strains isolated from pertussis patients in Moscow.


METHODS
53 strains of B. pertussis isolated from pertussis patients in Moscow in 2007 - 2010 as well as 3 vaccine strains currently used in Russia for the production of DTP vaccine were studied by multilocus sequencing (MLST) based on allele combinations of ptxA, ptxC and tcfA genes.


RESULTS
A genetic characteristic of B. pertussis strains isolated from pertussis patients in Moscow by using MLST is presented. Allele profile analysis of the studied B. pertussis strains was performed, 4 sequence types (ST) were identified--ST1, ST2, ST3 and ST5, most of the circulating strains (86.7%) were shown to belong to ST5, equal percentage of cases (5.7%)--to ST2 and ST3, and 1.9%--to ST1, while 2 vaccine production strains belong to ST2 and 1 - to ST1.


CONCLUSIONS
Clonal structure of contemporary Moscow strains was shown to be different from strain structure used for the production of DTP vaccine.
O Iu
OIu
borisova
I K
IK
Mazurova
G A
GA
Ivashinnikova
N T
NT
Gadua
I A
IA
Rudakova
N Ia
NIa
Salova
I P
IP
Trebunskikh
V G
VG
Skachkova
R A
RA
Panferova
F
F
Mooi
V A
VA
Aleshkin
D000483:Alleles; D001886:Bordetella pertussis / Q000235:genetics* / Q000302:isolation & purification*; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014917:Whooping Cough / Q000235:genetics* / Q000382:microbiology
D015721:Diphtheria-Tetanus-Pertussis Vaccine