SNP genotyping identifies new signatures of selection in a deep sample of West African Plasmodium falciparum malaria parasites.
We used a high-density single-nucleotide polymorphism array to genotype 75 Plasmodium falciparum isolates recently collected from Senegal and The Gambia to search for signals of selection in this malaria endemic region. We found little geographic or temporal stratification of the genetic diversity among the sampled parasites. Through application of the iHS and REHH haplotype-based tests for positive selection, we found evidence of recent selective sweeps at a known drug resistance locus, at several known antigenic loci, and at several genomic regions not previously identified as sites of recent selection. We discuss the value of deep population-specific genomic analyses for identifying selection signals within sampled endemic populations of parasites, which may correspond to local selection pressures such as distinctive therapeutic regimes or mosquito vectors.
Alfred
A
Amambua-Ngwa
Daniel J
DJ
Park
Sarah K
SK
Volkman
Kayla G
KG
Barnes
Amy K
AK
Bei
Amanda K
AK
Lukens
Papa
P
Sene
Daria
D
Van Tyne
Daouda
D
Ndiaye
Dyann F
DF
Wirth
David J
DJ
Conway
Daniel E
DE
Neafsey
Stephen F
SF
Schaffner
D000818:Animals; D005714:Gambia; D017125:Genes, Protozoan / Q000235:genetics; D005838:Genotype; D060005:Genotyping Techniques; D016778:Malaria, Falciparum / Q000469:parasitology*; D010271:Parasites / Q000235:genetics* / Q000302:isolation & purification; D010963:Plasmodium falciparum / Q000235:genetics* / Q000302:isolation & purification; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D025341:Principal Component Analysis; D012641:Selection, Genetic; D012675:Senegal