Selective blockade of apoptosis by in vivo electroporation-mediated gene transfer combined with portal infusion of plasmid DNA attenuates liver cirrhosis.
OBJECTIVE
The purpose of the present study was to determine whether in vivo electroporation could achieve selective blockade of apoptosis in a rat liver cirrhosis model.


METHODS
A dimethylnitrosamine (DMN)-induced rat liver cirrhosis model was used. In vivo electroporation was performed after portal vein infusion of plasmid DNA. pFas-Fc plasmid DNA was used to block the apoptotic pathway. pUC/HGF and pCAGGS/EGFP were used as positive and negative controls, respectively. Liver collagen content was evaluated by hydroxyproline assay two weeks after gene transfer. Terminal deoxynucleotidyltransferase dUTP nick end-labeling was simultaneously performed in the liver to evaluate suppression of apoptosis. Survival analysis was performed using 10 rats that received the sFas gene, 10 that received the HGF gene, and 13 that received the GFP gene.


RESULTS
The apoptotic cell index in the DMN-injected liver was significantly lower in rats that received the sFas gene compared with the negative control. The collagen content of the DMN-injected liver was also lower in rats that received the sFas gene compared with the negative control. There was no significant difference in the apoptotic cell index and collagen content of rats that received the sFas and HGF genes. Ten weeks after the initiation of DMN treatment, the survival rates with the sFas, HGF, and GFP genes were 56%, 100%, and 0, respectively.


CONCLUSIONS
Selective blockade of apoptosis by in vivo electroporation-mediated gene transfer improved the apoptotic cell index, hydroxyproline content, and survival rate. Soluble Fas gene therapy using in vivo electroporation can be a safe and efficient therapy for liver cirrhosis in rats.
Department of General and Cardiothoracic Surgery, Gifu University, Gifu, Japan. miyakanabon@yahoo.co.jp
T
T
Miyahara
Y
Y
Umeda
S
S
Yoshikawa
Y
Y
Matsuno
H
H
Iwata
H
H
Takemura
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D004247:DNA / Q000008:administration & dosage*; D018274:Electroporation; D018014:Gene Transfer Techniques; D007262:Infusions, Intravenous; D008103:Liver Cirrhosis / Q000235:genetics* / Q000517:prevention & control*; D008297:Male; D010957:Plasmids; D011169:Portal Vein; D051381:Rats; D017207:Rats, Sprague-Dawley
D004247:DNA