Expansion of a plasmid classification system for Gram-positive bacteria and determination of the diversity of plasmids in Staphylococcus aureus strains of human, animal, and food origins.
An expansion of a previously described plasmid classification was performed and used to reveal the plasmid content of a collection of 92 Staphylococcus aureus strains of different origins. rep genes of other genera were detected in Staphylococcus. S1 pulsed-field gel electrophoresis (PFGE) hybridizations were performed with 18 representative S. aureus strains, and a high number of plasmids of different sizes and organizations were detected.
Area Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.
Carmen
C
Lozano
Lourdes
L
García-Migura
Carmen
C
Aspiroz
Myriam
M
Zarazaga
Carmen
C
Torres
Frank Møller
FM
Aarestrup
D000818:Animals; D004265:DNA Helicases / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D010957:Plasmids / Q000145:classification* / Q000302:isolation & purification; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D015534:Trans-Activators / Q000235:genetics
D004269:DNA, Bacterial; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases