Response of ammonia oxidizing bacteria and archaea to acute zinc stress and different moisture regimes in soil.
Ammonia oxidation has been intensively studied for its sensitivity to environmental shifts and stresses. However, acute stress effects on the occurrence and composition of ammonia oxidizing bacteria (AOB) and archaea (AOA) based on expression of related molecular markers in complex soil environments have been to an extent overlooked, particularly concerning transient but commonly occurring environmental changes like soil moisture shifts. The present study investigates the responses of AOB and AOA to moisture shifts and high Zn soil content. AmoA gene copies and transcripts of AOB and AOA along with potential nitrification activity were measured in a soil microcosm approach for investigating the referred environmental shifts. Moisture change from 87 to 50 % of the water holding capacity caused a ~99 % reduction of AOB but not of AOA amoA transcripts that did not change significantly. Increasing applied zinc concentrations resulted in a reduction of potential nitrification rates and negatively affected studied gene expressions of both AOB and AOA, with AOB being more responsive. Both 16 S rRNA and amoA transcripts of AOB had an inverse relation to the applied zinc, indicating a gradual loss in total cell activity. Our results suggest the existence of pronounced differences between AOB and AOA concerning ammonia oxidation activity.
Institute of Agricultural and Environmental Chemistry, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, Italy.
Sotirios
S
Vasileiadis
Damiano
D
Coppolecchia
Edoardo
E
Puglisi
Annalisa
A
Balloi
Francesca
F
Mapelli
Rebecca E
RE
Hamon
Daniele
D
Daffonchio
Marco
M
Trevisan
D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018407:Bacterial Physiological Phenomena; D019641:DNA, Archaeal / Q000032:analysis / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D017753:Ecosystem; D018869:Heat-Shock Response; D058465:Nitrification; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics; D016133:Polymerase Chain Reaction; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014867:Water; D015032:Zinc / Q000032:analysis / Q000494:pharmacology*
D019641:DNA, Archaeal; D004269:DNA, Bacterial; D012987:Soil; D014867:Water; D000641:Ammonia; D010088:Oxidoreductases; C045582:ammonia monooxygenase; D015032:Zinc