Human platelet antigen genotypes in Turkish and Caucasian blood donors in Germany.
Exposition to allogenic human platelet antigens (HPAs) can lead to antibody formation causing neonatal alloimmune thrombocytopenia (NAIT), post-transfusion purpura or platelet (PLT) transfusion refractoriness. The frequencies of HPA differ between ethnical groups which could be associated with different potential alloimmunization risk. The Turkish population is the largest ethnic minority group living in Germany. However, no data are available about the HPA frequency among Turkish population. We compared the frequency of HPA between Caucasian and Turkish blood donors. DNA from blood samples of 119 Caucasian and 117 Turkish blood donors was isolated. The genotype of HPA-1, -2, -3 -4, -5 and -15 was determined using a commercialized polymerase chain reaction kit with sequence-specific primers. In Turkish blood donors, the gene frequencies of HPA-1a/1b, -2a/2b, -3a/3b, -4a/4b, -5a/5b and -15a/15b were 0.863/0.137, 0.868/0.133, 0.607/0.393, 0.996/0.004, 0.893/0.107 and 0.474/0.256, respectively. In Caucasians, we observed 0.798/0.202, 0.908/0.092, 0.567/0.432, 1.000/0.000, 0.916/0.084 and 0.517/0.483 for HPA-1a/1b, -2a/2b, -3a/3b, -4a/4b, -5a/5b and -15a/15b, respectively. No statistically significant difference between genotypes in these populations could be observed. Due to the similar distribution of HPA genotypes in both ethnical groups, no increased risk of NAIT for children of mixed couples or of post-transfusion purpura or PLT transfusion refractoriness secondary to antibodies to HPAs for recipients of PLT concentrates from blood donors of the other ethnicity is given.
Department of Transfusion Medicine and Hemostaseology, University Hospital Erlangen, Erlangen, Germany. barbara.hauck@uk-erlangen.de
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B
Hauck-Dlimi
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Hammon
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R
Eckstein
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Ott
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Zimmermann
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Dengler
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Ringwald
D016824:Antigens, Human Platelet / Q000235:genetics*; D001782:Blood Donors; D001803:Blood Transfusion; D005220:Fathers; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D005858:Germany; D006801:Humans; D007114:Immunization; D008297:Male; D009035:Mothers; D011247:Pregnancy; D012307:Risk Factors; D014421:Turkey / Q000208:ethnology; D044465:White People / Q000235:genetics*
D016824:Antigens, Human Platelet