A new rhodamine-based "off-on" fluorescent chemosensor for Hg (II) ion and its application in imaging Hg (II) in living cells.
A novel rhodamine derivative (Rh-C), synthesized by the reaction of rhodamine ethylenediamine and cinnamoyl chloride, was evaluated as a chemoselective Hg(2+) ion sensor. Addition of Hg(2+) to an ethanol aqueous solution of the Rh-C resulted in a color change from colorless to obvious pink color together with distinctive changes in UV-vis absorption spectrum and fluorescence spectrum. However, other common alkali-, alkaline earth-, transition- and rare earth metal ions induced no or minimal spectral changes. The interaction of Hg(2+) and sensor Rh-C was proven to adopt a 1:1 binding stoichiometry and the recognition process is reversible. The chemosensor displayed a linear response to Hg(2+) in the range of 0.4-5 μM with a detection limit of 7.4 × 10(-8) M. The sensor Rh-C was also successfully applied to the imaging of Hg(2+) in HL-7702 cells.
State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin 300160, China.
Fanyong
F
Yan
Donglei
D
Cao
Meng
M
Wang
Ning
N
Yang
Qiuhua
Q
Yu
Linfeng
L
Dai
Li
L
Chen
D002460:Cell Line; D002470:Cell Survival; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D004785:Environmental Pollutants / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008628:Mercury / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D004785:Environmental Pollutants; D012235:Rhodamines; D008628:Mercury