Isolation and detection of small RNAs from plant tissues.
In plants, several classes of non-coding small RNA (sRNA) have been shown to be important regulators of gene expression in a wide variety of biological processes. The two main classes of sRNA, the small-interfering RNA (siRNA) and microRNA (miRNA) classes, are well documented and several experimental approaches have been developed to allow for their routine isolation and detection from plant tissues. Here, we describe the current methods used for the isolation of total RNA and the subsequent enrichment of low-molecular-weight (LMW) RNA species, as well as to outline how sRNAs are detected from such nucleic acid preparations.
Division of Plant Industry, CSIRO, Canberra, ACT, Australia. neil.smith@csiro.com.au
Neil A
NA
Smith
Andrew L
AL
Eamens
D017360:Arabidopsis / Q000235:genetics; D001467:Hordeum / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000302:isolation & purification*; D012275:Oryza / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D034741:RNA, Small Interfering / Q000235:genetics / Q000302:isolation & purification*; D014026:Nicotiana / Q000235:genetics; D014908:Triticum / Q000235:genetics
D035683:MicroRNAs; D018749:RNA, Plant; D034741:RNA, Small Interfering