Microtubule stability affects the unique motility of F-actin in Marchantia               polymorpha.
Actin microfilaments play crucial roles in diverse plant functions. Some specific cellular processes require interaction between F-actin and microtubules, and it is believed that there are direct or indirect connections between F-actin and microtubules. We previously reported that actin microfilaments exhibit unique dynamic motility in cells of the liverwort, Marchantia polymorpha; the relevance of this activity to microtubules has not been explored. To examine whether the dynamics of F-actin in M. polymorpha were somehow regulated by microtubules, we investigated the effects of stabilization or destabilization of microtubules on dynamics of actin bundles, which were visualized by Lifeact-Venus. To our surprise, both stabilization and destabilization of microtubules exerted similar effects on F-actin motility; apparent sliding movement of F-actin in M. polymorpha cells was accelerated by both oryzalin and paclitaxel, with the effect of paclitaxel more evident than that of oryzalin. Immunofluorescence staining revealed that some F-actin bundles were arrayed along with microtubules in M. polymorpha thallus cells. These results suggest that microtubules play regulatory roles in the unique F-actin dynamics in M. polymorpha.
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Atsuko
A
Era
Natsumaro
N
Kutsuna
Takumi
T
Higaki
Seiichiro
S
Hasezawa
Akihiko
A
Nakano
Takashi
T
Ueda
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D001692:Biological Transport; D004136:Dinitrobenzenes / Q000494:pharmacology; D047008:Marchantia / Q000166:cytology* / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology*; D013424:Sulfanilamides / Q000494:pharmacology; D050257:Tubulin Modulators / Q000494:pharmacology
D000199:Actins; D004136:Dinitrobenzenes; D013424:Sulfanilamides; D050257:Tubulin Modulators; C012465:oryzalin; D017239:Paclitaxel