Induction of type I interferons by a novel porcine reproductive and respiratory syndrome virus isolate.
Porcine reproductive and respiratory syndrome virus (PRRSV) is known to interfere with the signaling of type I interferons (IFNs). Here we found PRRSV A2MC2 induced type I IFNs in cultured cells. A2MC2 replication in MARC-145 cells resulted in the synthesis of IFN-α2 protein, transcript elevation of the IFN-stimulated genes ISG15 and ISG56, and the proteins of the signal transducer and activator of transcription 2 (STAT2) and ISG56. A2MC2 infection of primary porcine pulmonary alveolar macrophages (PAMs) also led to the elevation of the two proteins, but had little cytopathic effect. Furthermore, A2MC2 infection of MARC-145 or PAM cells had no detectable inhibitory effect on the ability of IFN-α to induce an antiviral response. Sequencing analysis indicated that A2MC2 was closely related to VR-2332 and Ingelvac PRRS MLV with an identity of 99.8% at the nucleotide level. The identification of this IFN-inducing PRRSV isolate may be beneficial for vaccine development against PRRS.
Molecular Virology Laboratory, VA-MD Regional College of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742, USA.
Yuchen
Y
Nan
Rong
R
Wang
Meiyan
M
Shen
Kay S
KS
Faaberg
Siba K
SK
Samal
Yan-Jin
YJ
Zhang
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005838:Genotype; D007370:Interferon Type I / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D050795:STAT2 Transcription Factor / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013552:Swine; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism; D014709:Vero Cells; D010948:Viral Plaque Assay
D007370:Interferon Type I; D050795:STAT2 Transcription Factor; D014157:Transcription Factors; D014452:Ubiquitins