Dietary fish oil substitution alters the eicosanoid profile in ankle joints of mice during Lyme infection.
Dietary ingestion of (n-3) PUFA alters the production of eicosanoids and can suppress chronic inflammatory and autoimmune diseases. The extent of changes in eicosanoid production during an infection of mice fed a diet high in (n-3) PUFA, however, has not, to our knowledge, been reported. We fed mice a diet containing either 18% by weight soybean oil (SO) or a mixture with fish oil (FO), FO:SO (4:1 ratio), for 2 wk and then infected them with Borrelia burgdorferi. We used an MS-based lipidomics approach and quantified changes in eicosanoid production during Lyme arthritis development over 21 d. B. burgdorferi infection induced a robust production of prostanoids, mono-hydroxylated metabolites, and epoxide-containing metabolites, with 103 eicosanoids detected of the 139 monitored. In addition to temporal and compositional changes in the eicosanoid profile, dietary FO substitution increased the accumulation of 15-deoxy PGJ(2), an antiinflammatory metabolite derived from arachidonic acid. Chiral analysis of the mono-hydroxylated metabolites revealed they were generated from primarily nonenzymatic mechanisms. Although dietary FO substitution reduced the production of inflammatory (n-6) fatty acid-derived eicosanoids, no change in the host inflammatory response or development of disease was detected.
Departments of Chemistry, Biochemistry, and Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Darren S
DS
Dumlao
Anna M
AM
Cunningham
Laura E
LE
Wax
Paul C
PC
Norris
Jennifer Hughes
JH
Hanks
Rachel
R
Halpin
Kawasi M
KM
Lett
Victoria A
VA
Blaho
William J
WJ
Mitchell
Kevin L
KL
Fritsche
Edward A
EA
Dennis
Charles R
CR
Brown
D000821:Animal Feed; D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D015777:Eicosanoids / Q000378:metabolism*; D005227:Fatty Acids / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage / Q000494:pharmacology*; D006614:Hindlimb; D006358:Hot Temperature; D007596:Joints / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008193:Lyme Disease / Q000178:diet therapy* / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H
D004042:Dietary Fats, Unsaturated; D015777:Eicosanoids; D005227:Fatty Acids; D005395:Fish Oils