Improvement of the growth defect in salt- and ethanol-tolerant yeast mutagenized with error-prone DNA polymerization by using backcross cell fusion.
Salt- and ethanol-tolerant mutants of Saccharomyces cerevisiae, isolated from the uracil-requiring mutant derived from Taiken No. 396 by proofreading-deficient DNA polymerization, showed less growth than their parent strain. The fusants, between these tolerant mutants and the lysine-requiring mutant from Taiken No. 396 obtained by the protoplast fusion, indicated improved growth.
Division of Applied Life Science, Graduate School of Applied and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
Kazukiyo
K
Hayashi
Shuntaro
S
Yano
Hiroko
H
Abe
Yasuko
Y
Fujita
Masao
M
Kishida
D004258:DNA Polymerase III / Q000235:genetics; D000431:Ethanol; D007218:Industrial Microbiology; D016296:Mutagenesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D012965:Sodium Chloride
D000431:Ethanol; D012965:Sodium Chloride; D004258:DNA Polymerase III