Tissue protection and endothelial cell signaling by 20-HETE analogs in intact ex vivo lung slices.
The capacity to follow cell type-specific signaling in intact lung remains limited. 20-hydroxyeicosatetraenoic acid (20-HETE) is an endogenous fatty acid that mediates signaling for a number of key physiologic endpoints in the pulmonary vasculature, including cell survival and altered vascular tone. We used confocal microscopy to identify enhanced reactive oxygen species (ROS) production in endothelial cell (EC)s in intact lung evoked by two stable analogs of 20-HETE, 20-5,14-HEDE (20-hydroxyeicosa-5(Z),14(Z)-dienoic acid) and 20-5,14-HEDGE (N-[20-hydroxyeicosa-5(Z),14(Z)-dienoyl]glycine). These analogs generated increased ROS in cultured pulmonary artery endothelial cells as well. 20-HETE analog treatment decreased apoptosis of pulmonary tissue exposed to hypoxia-reoxygenation (HR) ex vivo. Enhanced ROS production and apoptosis were confirmed by biochemical assays. Our studies identify physiologically critical, graded ROS from ECs in live lung tissue ex vivo treated with 20-HETE analogs and protection from HR-induced apoptosis. These methodologies create exciting possibilities for studying signaling by stable 20-HETE analogs and other factors in pulmonary endothelial and other lung cell types in their native milieu.
Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, United States. Elizabeth.Jacobs@va.gov
Elizabeth R
ER
Jacobs
Sreedhar
S
Bodiga
Irshad
I
Ali
Aaron M
AM
Falck
John R
JR
Falck
Meetha
M
Medhora
Anuradha
A
Dhanasekaran
D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D000860:Hypoxia / Q000378:metabolism; D055666:Lipopeptides / Q000494:pharmacology*; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018613:Microscopy, Confocal; D010100:Oxygen / Q000494:pharmacology; D061251:Primary Cell Culture; D011651:Pulmonary Artery / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D046509:Tissue Culture Techniques
C494665:20-hydroxyeicosa-5(Z),14(Z)-dienoic acid; D006893:Hydroxyeicosatetraenoic Acids; D055666:Lipopeptides; C533498:N-(20-hydroxyeicosa-5,14-dienoyl)glycine; D017382:Reactive Oxygen Species; C055987:20-hydroxy-5,8,11,14-eicosatetraenoic acid; D010100:Oxygen