Expression, purification and crystallization of the Crimean-Congo haemorrhagic fever virus nucleocapsid protein.
Crimean-Congo haemorrhagic fever virus (CCHFV) is a member of the Nairovirus genus within the Bunyaviridae family of segmented negative-sense RNA viruses. This paper describes the expression, purification and crystallization of full-length CCHFV nucleocapsid (N) protein and the collection of a 2.1 Å resolution X-ray diffraction data set using synchrotron radiation. Crystals of the CCHFV N protein belonged to space group C2, with unit-cell parameters a = 150.38, b = 72.06, c = 101.23 Å, β = 110.70° and two molecules in the asymmetric unit. Circular-dichroism analysis provided insight into the secondary structure, whilst gel-filtration analysis revealed possible oligomeric states of the N protein. Structural determination is ongoing.
Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, England.
S D
SD
Carter
J N
JN
Barr
T A
TA
Edwards
D002942:Circular Dichroism; D003460:Crystallization; D015870:Gene Expression; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000737:chemistry*; D019590:Nucleocapsid Proteins / Q000737:chemistry* / Q000302:isolation & purification
D019590:Nucleocapsid Proteins