Molecular diagnosis of CMV infection in fetal aborted tissues in the region of Thrace.
OBJECTIVE
To detect the incidence of CMV infection in spontaneous abortion in Thrace.


METHODS
Genetic material from 143 fetuses aged from 11 to 39 weeks was examined. The material originated from various regions of Thrace. All fetuses and the respective placentas underwent routine histopathology. DNA was isolated from sections of paraffinized tissues. Detection of CMV in the DNA genomic samples was performed using a commercial PCR-based detection kit.


RESULTS
From the 143 fetuses that were examined, two were found to be CMV positive. Pathological findings related to inflammatory corruptions were observed in the placentas of 97 embryos, including the CMV infected ones.


CONCLUSIONS
This study indicates CMV-DNA infection in 1.4% of aborted fetuses. CMV infection incidence in aborted fetuses is similar to this reported in other European regions. The molecular technique of PCR applied on paraffin-embedded biopsy material is proven to be an accurate, valid and fast method for investigating the CMV infection in aborted fetuses.
Laboratory of Histology-Embryology, Medical School, Democritus University of Thrace, Greece.
I
I
Grammatikopoulou
M
M
Lambropoulou
E
E
Chatzaki
T E
TE
Deftereou
V
V
Lambropoulou
M
M
Simopoulou
E
E
Papadopoulos
G
G
Galazios
Th
T
Dimitriou
A
A
Petrou
N
N
Papadopoulos
D037881:Aborted Fetus / Q000473:pathology; D000022:Abortion, Spontaneous / Q000473:pathology / Q000821:virology*; D000293:Adolescent; D000328:Adult; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000150:complications*; D005260:Female; D006115:Greece; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D055815:Young Adult