Polyherbal formulation Bresol® protects the mast cells against compound 48/80-induced disruption and histamine release: a non-immunological mechanism of mast cell stabilization.
OBJECTIVE
Present study was aimed to evaluate the protective effect of Bresol®, a polyherbal formulation, on mast cell degranulation and histamine release from mast cells.


METHODS
Mast cell-stabilizing activity of Bresol® was evaluated against compound 48/80-induced mast cell degranulation and histamine release from rat peritoneal mast cells in ex vivo conditions.


RESULTS
Microscopy of the control group smears showed more of intact mast cells, with very minimum number of degranulated mast cells and negligible amount of histamine release. In contrast, incubation of mast cells with compound 48/80 caused significant degranulation of the mast cells associated with release of high concentration of histamine in the positive control group. Furthermore, Bresol® at 100 mg/L showed a significant inhibition of compound 48/80-induced mast cell degranulation. In addition, Bresol® significantly and dose-dependently inhibited compound 48/80-induced histamine release.


CONCLUSIONS
Bresol® inhibits compound 48/80-induced mast cell degranulation and histamine release in ex vivo conditions. The present findings could be one of the non-immunological mechanism responsible for usefulness of Bresol® in various allergic conditions.
Department of Pharmacology, Research and Development Center, the Himalaya Drug Company, Bangalore 562123, Karnataka, India.
Yathiraj
Y
Ashwini
Mohamed
M
Rafiq
Gollapalle L
GL
Viswanatha
Subbanna
S
Rajesh
Selvam
S
Senthilraja
Mohammed
M
Azeemuddin
Suryakanth D
SD
Anturlikar
Paramesh
P
Rangesh
Pralhad S
PS
Patki
D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D005089:Exocytosis / Q000187:drug effects; D006636:Histamine Release / Q000187:drug effects*; D008407:Mast Cells / Q000187:drug effects*; D028321:Plant Preparations / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
C570562:Bresol; D028321:Plant Preparations; D003189:p-Methoxy-N-methylphenethylamine