Differential effects of nerve growth factor on expression of dopamine 2 receptor subtypes in GH3 rat pituitary tumor cells.
Nerve growth factor (NGF) can increase expression of dopamine 2 receptors (D2R) in GH3 rat pituitary tumor cells (GH3 cells). As D2R exists in long (D2L) and short (D2S) isoforms, effects of NGF on D2R subtypes have not been accurately evaluated. In this study, we compared mRNA levels of D2R subtypes in GH3 cells treated with or without NGF with real-time RT-PCR. In addition, we also evaluated the relationship between GH3 cell growth after bromocriptine treatment and mRNA levels of D2R subtypes. We found that D2R total, D2L, and D2S mRNA in GH3 cells were significantly increased after NGF treatment, compared with the vehicle group. Moreover, NGF increased the ratio of D2S to D2L. GH3 cell survival rate after bromocriptine treatment was negatively correlated with D2R total mRNA, and D2S may be more potent than D2L in inhibiting cell growth. Cell apoptosis rate was highly elevated in GH3 cells treated with NGF and bromocriptine, compared with the control group or the group treated with NGF or bromocriptine alone. Our data provide preliminary evidence that the effect of NGF was more prominent on expression of D2S than D2L, in addition, D2S might have a greater impact suppressing GH3 cells growth than D2L.
Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, China.
Zhipeng
Z
Su
Xiaolong
X
Jiang
Chengde
C
Wang
Jie
J
Liu
Yunxiang
Y
Chen
Qun
Q
Li
Jinsen
J
Wu
Weiming
W
Zheng
Qichuan
Q
Zhuge
Kunlin
K
Jin
Zhebao
Z
Wu
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001971:Bromocriptine / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005434:Flow Cytometry; D020932:Nerve Growth Factor / Q000494:pharmacology*; D010911:Pituitary Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D017448:Receptors, Dopamine D2 / Q000819:agonists / Q000096:biosynthesis*
D012333:RNA, Messenger; D017448:Receptors, Dopamine D2; D001971:Bromocriptine; D020932:Nerve Growth Factor