Kisspeptin-10 stimulates the release of luteinizing hormone and testosterone in pre- and post-pubertal male goats.
The aims of the present study were to clarify the effect of kisspeptin-10 (Kp10) on the secretion of luteinizing hormone (LH) and testosterone (T) in pre-pubertal and post-pubertal male ruminants. Four male goats (Shiba goats) were given an intravenous (i.v.) injection of Kp10 (5 µg/kg body weight (b.w.)), gonadotoropin-releasing hormone (GnRH, 1 µg/kg b.w.), or 2 mL of saline as a control at the ages of 3 (pre-pubertal) and 6 (post-pubertal) months. A single i.v. injection of Kp10 significantly stimulated the release of LH and T in both groups. The area under the response curve (AUC) of LH for a 60-min period after the i.v. injection of Kp10 was significantly greater in the pre-pubertal goats (P < 0.05). The AUC of T for a 120 min period post-injection did not differ between the two age groups. A single i.v. injection of GnRH also significantly stimulated the release of LH and T in both groups (P < 0.05). The secretory pattern of LH and T in response to GnRH resembled that in response to Kp10. These results show that the LH-releasing response to Kp10 is greater in pre-pubertal than post-pubertal male goats. They also show that Kp10, as well as GnRH, is able to stimulate the release of T in male goats.
Faculty of Agriculture, Iwate University, Morioka, Japan.
Hayato
H
Saito
Tatsuru
T
Sawada
Tomoyoshi
T
Yaegashi
Yuki
Y
Goto
Jin
J
Jin
Ken
K
Sawai
Tsutomu
T
Hashizume
D000818:Animals; D006041:Goats / Q000502:physiology*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007275:Injections, Intravenous; D059648:Kisspeptins / Q000008:administration & dosage* / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D011627:Puberty / Q000502:physiology*; D013268:Stimulation, Chemical; D013739:Testosterone / Q000378:metabolism*
C106628:KISS1 protein, human; D059648:Kisspeptins; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D007986:Luteinizing Hormone