[Cloning and functional analysis of SCTF-1 encoding a C2H2-type Zinc finger protein from soybean].
The zinc finger protein is one of the proteins with finger-like domain. Some of them are transcription factors which play important role in plant growth and plant resistance to abiotic stresses. In this paper, a novel C2H2-type zinc finger protein gene SCTF-1 (GenBank accession number JQ692081) was isolated from soybean (Glycine max (L.) Merr.) This gene has a 699 bp ORF (open reading frame) with no intron and encodes a 24.9 kDa protein with 233 amino acids. Its isoelectric point (pI) is 8.33. The SCTF-1 protein contains two typical C2H2-type zinc finger domains. Both of them have highly conserved amino acid sequence-QALGGH which is a particular characteristic of plant. Transient expression of the GFP-SCTF-1 protein in onion epidermal cell showed that SCTF-1 was localized in cell nuclei. RT-PCR results showed that SCTF-1 gene was expressed with high levels in flowers and leaves in soybean, but low in roots and stems. The expression of SCTF-1 gene was strongly induced by low temperature in the soybean seedlings. Overexpression of SCTF-1 enhanced cold tolerance of transgenic tobacco (Nicotiana tabacum L.) compared to the control.
College of Agriculture, Jilin Agricultural University, Changchun 130118, China. song19800123@126.com
Bing
B
Song
Pi-Wu
PW
Wang
Yong-Ping
YP
Fu
Xu-Hong
XH
Fan
Hai-Feng
HF
Xia
Wei
W
Gao
Yang
Y
Hong
He
H
Wang
Zhuo
Z
Zhang
Jian
J
Ma
D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism*; D016335:Zinc Fingers / Q000235:genetics*
D010940:Plant Proteins