Immunohistochemical and morphometric evaluation of neuronal dysfunction in pelviureteral junction obstruction.
OBJECTIVE
The pathophysiology of pelviureteral junction obstruction remains unclear. In this study, we attempted to evaluate the neuronal dysfunction using immunohistochemical and morphometric parameters.


METHODS
Using specific antibodies, we studied the neuronal markers and morphometry of specimens from 10 cases of pelviureteral junction obstruction and 10 normal pelviureteral junctions by immunohistochemistry using synaptophysin (synaptic vesicle membrane protein), S-100 (nerve cell fiber marker), protein gene product 9.5 (neuron specific protein) and CD-117 (transmembrane receptor with tyrosine kinase activity).


RESULTS
In pelviureteral junction obstruction, smooth muscle thickness was significantly higher than in normal specimens. Cytoplasmic cells stained by synaptophysin were found in the pelviureteral junction obstruction specimens but were absent in normal pelviureteral junction specimens. In addition, significantly more intense staining for S-100 was found in the pelviureteral junction obstruction specimens compared with the normal specimens. Immunohistochemical staining for protein gene product 9.5 showed no differences between the normal and pelviureteral junction obstruction specimens. Neither the normal specimens nor pelviureteral junction obstruction groups were stained by CD-117.


CONCLUSIONS
We propose that the cause of neuronal dysfunction in pelviureteral junction obstruction depends on the increase in number and structure of neuronal cells and smooth muscle thickness. These factors could play an important role in the pathophysiology of pelviureteral junction obstruction by affecting motility and peristalsis.
Department of Pediatric Surgery, Meram Medical School of Selçuk University, Konya, Turkey. drmetingunduz@yahoo.com
Metin
M
Gündüz
Müslim
M
Yurtçu
Hatice
H
Toy
Adnan
A
Abasiyanik
Serafettin
S
Demirci
D014408:Biomarkers, Tumor; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007682:Kidney Pelvis; D008297:Male; D009130:Muscle, Smooth / Q000294:innervation / Q000473:pathology; D009418:S100 Proteins; D016708:Synaptophysin; D043222:Ubiquitin Thiolesterase; D014517:Ureteral Obstruction / Q000503:physiopathology* / Q000601:surgery
D014408:Biomarkers, Tumor; D009418:S100 Proteins; C569014:SYP protein, human; D016708:Synaptophysin; C482860:UCHL1 protein, human; D043222:Ubiquitin Thiolesterase