In ovo eye electroporation.
Electroporation has been used successfully to introduce macromolecules such as DNA into the chick embryo for at least 15 years. Purified plasmid DNA is microinjected into embryo and then a series of low voltage electrical pulses are applied to the embryo which allows naked DNA to enter cells. Following entrance into the cytoplasm, the DNA is transported to the nucleus where it is transiently expressed. This powerful technique is useful for studies involving overexpression, misexpression, and knockdown of genes of interest at a variety of developmental timepoints.
Department of Biology & Center for Regenerative Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN, USA. tbadams@iupui.edu
Teri L
TL
Belecky-Adams
Scott R
SR
Hudson
Sarika
S
Tiwari
D000818:Animals; D002642:Chick Embryo; D004247:DNA / Q000378:metabolism; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D005123:Eye / Q000196:embryology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D008845:Microinjections; D019076:Transgenes
D004247:DNA