Overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to Cochliobolus sativus and Fusarium graminearum in transgenic wheat.
The fungus Cochliobolus sativus is the main pathogen of common root rot, a serious soil-borne disease of wheat (Triticum aestivum L.). The fungus Fusarium graminearum is the primary pathogen of Fusarium head blight, a devastating disease of wheat worldwide. In this study, the wheat lipid transfer protein gene, TaLTP5, was cloned and evaluated for its ability to suppress disease development in transgenic wheat. TaLTP5 expression was induced after C. sativus infection. The TaLTP5 expression vector, pA25-TaLTP5, was constructed and bombarded into Chinese wheat variety Yangmai 18. Six TaLTP5 transgenic wheat lines were established and characterized. PCR and Southern blot analyses indicated that the introduced TaLTP5 gene was integrated into the genomes of six transgenic wheat lines by distinct patterns, and heritable. RT-PCR and real-time quantitative RT-PCR revealed that the TaLTP5 gene was over-expressed in the transgenic wheat lines compared to segregants lacking the transgene and wild-type wheat plants. Following challenge with C. sativus or F. graminearum, all six transgenic lines overexpressing TaLTP5 exhibited significantly enhanced resistance to both common root rot and Fusarium head blight compared to the untransformed wheat Yangmai 18.
The National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Genetic and Breeding of the Agriculture Ministry, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Xiuliang
X
Zhu
Zhao
Z
Li
Huijun
H
Xu
Miaoping
M
Zhou
Lipu
L
Du
Zengyan
Z
Zhang
D052179:Antigens, Plant / Q000235:genetics* / Q000378:metabolism; D001203:Ascomycota / Q000276:immunology / Q000472:pathogenicity*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D060467:Disease Resistance; D005670:Fusarium / Q000276:immunology / Q000472:pathogenicity*; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D018745:Genome, Plant; D040582:Inheritance Patterns; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000276:immunology / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000276:immunology / Q000382:microbiology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D019076:Transgenes; D014908:Triticum / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; C511920:lipid transfer proteins, plant