Effect of mobile phones on micronucleus frequency in human exfoliated oral mucosal cells.
OBJECTIVE
In the last two decades, the use of mobile phones has increased enormously all over the world. The controversy regarding whether radiofrequency (RF) fields exert effects upon biological systems is a concern for the general population. An evaluation is made of DNA damage and cytokinetic defects, proliferative potential, and cell death because of RF radiation emitted by mobile phones in healthy young users.


METHODS
This cohort study was carried out in 50 Caucasian mobile phone users. We collected two cell samples from each subject (a total of 100 cell samples), corresponding to the right and left cheek mucosa, respectively. Case histories and personal information were assessed, including age, gender, body height and weight, history of cancer, smoking and alcohol consumption, exposure to chemical carcinogens or radiation, and dietary habits. Sampling comprised cell collection from both cheeks with a cytobrush, centrifugation, slide preparation, fixation, and staining, followed by fluorescent microscopic analysis. A total of 2000 exfoliated cells were screened for nuclear abnormalities, especially micronucleus.


RESULTS
No statistically significant changes were recorded in relation to age, gender, body mass index, or smoking status. A comparison of the results vs the control area according to the side of the face on which the mobile phone was placed, and in relation to the duration of exposure (years) to mobile phone radiation in the total 100 samples, yielded no significant differences.


CONCLUSIONS
No genotoxic effects because of RF exposure were observed in relation to any of the study parameters.
Department of Oral Medicine, Morales Meseguer Hospital, University of Murcia, Murcia, Spain.
I
I
Ros-Llor
M
M
Sanchez-Siles
F
F
Camacho-Alonso
P
P
Lopez-Jornet
D000328:Adult; D000428:Alcohol Drinking; D001827:Body Height; D015992:Body Mass Index; D001835:Body Weight; D002273:Carcinogens; D016923:Cell Death / Q000528:radiation effects; D040421:Cell Phone; D049109:Cell Proliferation / Q000528:radiation effects; D015331:Cohort Studies; D003581:Cytodiagnosis / Q000295:instrumentation; D004249:DNA Damage; D004574:Electromagnetic Fields / Q000009:adverse effects; D004781:Environmental Exposure; D005247:Feeding Behavior; D005260:Female; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000145:classification*; D015162:Micronucleus Tests; D008856:Microscopy, Fluorescence; D009061:Mouth Mucosa / Q000528:radiation effects* / Q000648:ultrastructure; D011846:Radio Waves / Q000009:adverse effects; D012907:Smoking; D055815:Young Adult
D002273:Carcinogens