Antimicrobial activity of alcohols from Musca domestica.
Information on the stimulatory and inhibitory effects of cuticular alcohols on growth and virulence of insecticidal fungi is unavailable. Therefore, we set out to describe the content of cuticular and internal alcohols in the body of housefly larvae, pupae, males and females. The total cuticular alcohols in larvae, males and females of Musca domestica were detected in comparable amounts (4.59, 3.95 and 4.03 μg g(-1) insect body, respectively), but occurred in smaller quantities in pupae (2.16 μg g(-1)). The major free alcohol in M. domestica larvae was C(12:0) (70.4%). Internal alcohols of M. domestica larvae were not found. Among cuticular pupae alcohols, C(12:0) (31.0%) was the most abundant. In the internal lipids of pupae, only five alcohols were identified in trace amounts. The most abundant alcohol in males was C(24:0) (57.5%). The percentage content of cuticular C(24:0) in males and females (57.5 and 36.5%, respectively) was significantly higher than that of cuticular lipids in larvae and pupae (0.9 and 5.6%, respectively). Only two alcohols were present in the internal lipids of males in trace amounts (C(18:0) and C(20:0)). The most abundant cuticular alcohols in females were C(24:0) (36.5%) and C(12:0) (26.8%); only two alcohols (C(18:0) and C(20:0)) were detected in comparable amounts in internal lipids (3.61±0.32 and 5.01±0.42 μg g(-1), respectively). For isolated alcohols, antimicrobial activity against 10 reference strains of bacteria and fungi was determined. Individual alcohols showed approximately equal activity against fungal strains. C(14:0) was effective against gram-positive bacteria, whereas gram-negative bacteria were resistant to all tested alcohols. Mixtures of alcohols found in cuticular lipids of larvae, pupae, males and females of M. domestica generally presented higher antimicrobial activity than individual alcohols. In contrast, crude extracts containing both cuticular and internal lipids showed no antifungal activity against the entomopathogenic fungus Conidiobolus coronatus, which efficiently kills adult house flies.
Institute for Environmental and Human Health Protection, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland. goleb@chem.univ.gda.pl
Marek
M
Gołębiowski
Małgorzata
M
Dawgul
Wojciech
W
Kamysz
Mieczysława I
MI
Boguś
Wioletta
W
Wieloch
Emilia
E
Włóka
Monika
M
Paszkiewicz
Elżbieta
E
Przybysz
Piotr
P
Stepnowski
D000438:Alcohols / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D004198:Disease Susceptibility; D005260:Female; D005658:Fungi / Q000187:drug effects; D006793:Houseflies / Q000187:drug effects* / Q000382:microbiology; D007814:Larva / Q000737:chemistry / Q000187:drug effects; D008055:Lipids / Q000737:chemistry; D008297:Male; D008752:Methylene Chloride / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D009181:Mycoses / Q000473:pathology; D010578:Petroleum; D011679:Pupa / Q000737:chemistry / Q000187:drug effects; D014020:Tissue Extracts
D000438:Alcohols; D000890:Anti-Infective Agents; D008055:Lipids; D010578:Petroleum; D014020:Tissue Extracts; D008752:Methylene Chloride