[Qualitative and quantitative analysis of flavonoids in Chinese hawthorn fruit].
OBJECTIVE
To develop an LC-MS method for identification of flavonoids in Chinese hawthorn fruits and an HPLC method for quantification of 5 flavonoids.


METHODS
Accurate molecular weights of the target flavonoids were acquired by time-of-flight mass spectrometer (TOF-MS) in both positive and negative ion modes. The flavoniods were then further confirmed by standards. An RP-HPLC method was established to quantitatively analysis five major flavonoids (chlorogenic acid, procyanidin B2, epicatechin, hyperoside and isoquercitrin) in hawthorn fruits from ten different regions.


RESULTS
The molecular formulae of 8 components were determined by TOF-MS. In the quantitative method validation, the method is linear over the studied range of 1.8-178.5, 2.2-284.8, 2.7-270. 6,1.5-150.0, 1.5-150.0 mg x L(-1) for chlorogenic acid, procyanidin B2, epicatechin, hyperoside and isoquercitrin, respectively. The correlation coefficient for each analyze was greater than 0.999. The inter-day precision of the analysis was lower than 4%, The RSDs of precision and stability were lower than 5%. The recovery rate was from 98.5% to 102.5%, and RSDs were less than 3.5%.


CONCLUSIONS
The method is simple, specific and reliable, and is suitable for quality control of Chinese hawthorn.
Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, China.
Hongyang
H
Zhang
Chi
C
Zhang
Yuerong
Y
Wang
Min
M
Zhang
Xin
X
Jin
Ping
P
Hu
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D027825:Crataegus / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry; D011786:Quality Control
D005419:Flavonoids