Competition between two virulent Marek's disease virus strains in vivo.
Previous studies have demonstrated the presence of multiple strains of Marek's disease virus simultaneously circulating within poultry flocks, leading to the assumption that individual birds are repeatedly exposed to a variety of virus strains in their lifetime. Virus competition within individual birds may be an important factor that influences the outcome of co-infection under field conditions, including the potential outcome of emergence or evolution of more virulent strains. A series of experiments was designed to evaluate virus competition within chickens following simultaneous challenge with two virulent serotype 1 Marek's disease virus strains, using either pathogenically similar (rMd5 and rMd5/pp38CVI) or dissimilar (JM/102W and rMd5/pp38CVI) virus pairs. Bursa of Fabricius, feather follicle epithelium, spleen, and tumour samples were collected at multiple time points to determine the frequency and distribution of each virus present using pyrosequencing, immunohistochemistry and virus isolation. In the similar pair, rMd5 appeared to have a competitive advantage over rMd5/pp38CVI, which in turn had a competitive advantage over the less virulent JM/102W in the dissimilar virus pair. Dominance of one strain over the other was not absolute for either virus pair, as the subordinate virus was rarely eliminated. Interestingly, competition between two viruses with either pair rarely ended in a draw. Further work is needed to identify factors that influence virus-specific dominance to better understand what characteristics favour emergence of one strain in chicken populations at the expense of other strains.
USDA, Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA. john.dunn@ars.usda.gov
John R
JR
Dunn
Robert F
RF
Silva
Lucy F
LF
Lee
Richard L
RL
Witter
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D060085:Coinfection / Q000821:virology*; D008381:Herpesvirus 2, Gallid / Q000145:classification / Q000472:pathogenicity*; D007150:Immunohistochemistry / Q000662:veterinary; D008380:Marek Disease / Q000821:virology*; D056265:Microbial Interactions / Q000235:genetics / Q000502:physiology*; D011157:Population Dynamics; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013045:Species Specificity; D018709:Statistics, Nonparametric; D014774:Virulence
D000911:Antibodies, Monoclonal