Correlation and clinical utility of pp65 antigenemia and quantitative polymerase chain reaction assays for detection of cytomegalovirus in pediatric renal transplant patients.
qPCR and pp65 antigenemia assays are used to monitor CMV infection in renal transplant recipients, but correlation of assays in a pediatric population has not been evaluated. Paired CMV real-time qPCR and pp65 antigenemia tests from 882 blood samples collected from 115 pediatric renal transplant recipients were analyzed in this retrospective cohort study for the strength of association and clinical correlates. The assays correlated well in detecting infection (κ = 0.61). Higher qPCR values were demonstrated with increasing levels of antigenemia (p < 0.01). Discordant test results were associated with antiviral treatment (OR 4.33, p < 0.01) and low-level viremia, with odds of concordance increasing at higher qPCR values (OR 3.67, p < 0.01), and no discordance occurring above 8500 genomic equivalents/mL. Among discordant samples, neither test preceded the other in detecting initial infection or in returning to negative while on treatment. Only two cases of disease occurred during the two-yr study period. With strong agreement in the detection of CMV infection, either qPCR or pp65 antigenemia assays can be used effectively for monitoring pediatric renal transplant patients for both detection and resolution of infection.
Department of Pediatrics Biostatistics, The University of Alabama, Birmingham, AL, USA. brha@peds.uab.edu
Brian
B
Rha
David
D
Redden
Mark
M
Benfield
Fred
F
Lakeman
Richard J
RJ
Whitley
Masako
M
Shimamura
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007223:Infant; D016030:Kidney Transplantation / Q000009:adverse effects / Q000379:methods*; D008297:Male; D010372:Pediatrics / Q000379:methods; D010750:Phosphoproteins / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D051437:Renal Insufficiency / Q000150:complications / Q000628:therapy*; D012189:Retrospective Studies; D014763:Viral Matrix Proteins / Q000276:immunology*
D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa