A rapid and facile signal enhancement method for microcantilever-based immunoassays using the agglomeration of ferromagnetic nanoparticles.
A rapid and facile signal enhancement method for detecting alpha-fetoprotein (AFP) was developed using the magnetic agglomeration of ferromagnetic nanoparticles and microcantilever sensors. The resonance frequency and deflection of the cantilevers were found to be more than 10-fold greater than that before physical agglomeration of the free nanoparticles around the magnetized nanoparticles.
Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea.
Dongkyu
D
Lee
Donghoon
D
Kwon
Wooree
W
Ko
Jinmyoung
J
Joo
Hyejung
H
Seo
Soo Suk
SS
Lee
Sangmin
S
Jeon
D055910:Antibodies, Immobilized / Q000276:immunology; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000295:instrumentation*; D059346:Magnets / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000191:economics / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
D055910:Antibodies, Immobilized; D000509:alpha-Fetoproteins