Magnetic nanoparticles modified with DTPA-AMC-rare earth for fluorescent and magnetic resonance dual mode imaging.
In the present study, we report new water-soluble cell fluorescence imaging and contrast agents that are based on DTPA-AMC(7-amino-4-methyl coumarin)-Eu(3+) and DTPA-AMC(7-amino-4-methyl coumarin)-Gd(3+) compounds conjugated to Fe(3)O(4) NPs via a PEG-NH(2) linker. The novel Fe(3)O(4) NP-conjugates present two main advantages for cell fluorescence labelling: water solubility and targeting ability. The in vitro experiments demonstrate that water-soluble Fe(3)O(4) NPs-DBI-PEG-NH-DTPA-AMC(7-amino-4-methyl coumarin)-Eu(3+) has excellent cell permeating activity. Moreover, the relaxation rate test of Fe(3)O(4) NPs-DBI-PEG-NH-DTPA-AMC(7-amino-4-methyl coumarin)-Gd(3+) shows a higher T1 relaxation effect than traditional DTPA-Gd(3+) MRI agents. According to in vivo liver MRI experiments, better contrast of the liver was achieved after addition of Fe(3)O(4) NPs-DBI-PEG-NH-DTPA-AMC(7-amino-4-methyl coumarin)-Gd(3+). The results will provide a significant guide for researchers exploring the biomedical applications of superparamagnetic Fe(3)O(4) NPs.
State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
Zengchen
Z
Liu
Bo
B
Li
Baodui
B
Wang
Zhengyin
Z
Yang
Qin
Q
Wang
Tianrong
T
Li
Dongdong
D
Qin
Yong
Y
Li
Mingfang
M
Wang
Mihui
M
Yan
D000818:Animals; D003287:Contrast Media / Q000737:chemistry* / Q000494:pharmacology; D003374:Coumarins / Q000737:chemistry* / Q000494:pharmacology; D005063:Europium / Q000737:chemistry* / Q000494:pharmacology; D005682:Gadolinium / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008099:Liver / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D004369:Pentetic Acid / Q000737:chemistry* / Q000494:pharmacology; D013050:Spectrometry, Fluorescence
D003287:Contrast Media; D003374:Coumarins; D058185:Magnetite Nanoparticles; D005063:Europium; D004369:Pentetic Acid; D005682:Gadolinium; C028743:7-amino-4-methylcoumarin