Cloning, production, and functional expression of the bacteriocin enterocin A, produced by Enterococcus faecium T136, by the yeasts Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha, and Arxula adeninivorans.
The bacteriocin enterocin A (EntA) produced by Enterococcus faecium T136 has been successfully cloned and produced by the yeasts Pichia pastoris X-33EA, Kluyveromyces lactis GG799EA, Hansenula polymorpha KL8-1EA, and Arxula adeninivorans G1212EA. Moreover, P. pastoris X-33EA and K. lactis GG799EA produced EntA in larger amounts and with higher antimicrobial and specific antimicrobial activities than the EntA produced by E. faecium T136.
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid (UCM), Madrid, Spain.
Juan
J
Borrero
Gotthard
G
Kunze
Juan J
JJ
Jiménez
Erik
E
Böer
Loreto
L
Gútiez
Carmen
C
Herranz
Luis M
LM
Cintas
Pablo E
PE
Hernández
D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics* / Q000494:pharmacology; D003001:Cloning, Molecular; D016984:Enterococcus faecium / Q000235:genetics* / Q000378:metabolism; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C107799:enterocin A