The effects of medical ozone therapy on renal ischemia/reperfusion injury.
BACKGROUND
This study was designed to investigate the possible beneficial effects of medical ozone therapy (OT), known as an immunomodulator and antioxidant, on the renal function, morphology, and biochemical parameters of oxidative stress in kidneys subjected to ischemia/reperfusion injury (IRI).


METHODS
Thirty male Sprague-Dawley rats were classified into three groups: control, renal IRI, and renal IRI + OT. The IRI group was induced by bilateral renal ischemia for 60 min, followed by reperfusion for 6 h. After reperfusion, the kidneys and blood of rats were obtained for histopathologic and biochemical evaluation.


RESULTS
Renal IRI increased the tissue oxidative stress parameters (lipid peroxidation, protein oxidation, and nitrite plus nitrate) and decreased the antioxidant enzyme activities (superoxide dismutase and glutathione peroxidase). The serum neopterin levels showed correlation with oxidative stress parameters. All these parameters were brought to control values in the treatment group. Histopathologically, the kidney injury in the treatment group was significantly lesser than in the renal IRI group.


CONCLUSIONS
Our results clearly showed that OT has beneficial effect to protect kidney against IRI. The serum neopterin levels might be used as a marker to detect the degree of renal IRI.
Health Services Command, Turkish Armed Forces, Ankara, Turkey. muzafferoztosun@yahoo.com
Muzaffer
M
Oztosun
Emin Ozgur
EO
Akgul
Erdinc
E
Cakir
Tuncer
T
Cayci
Bulent
B
Uysal
Recai
R
Ogur
Ayhan
A
Ozcan
Taner
T
Ozgurtas
Ahmet
A
Guven
Ahmet
A
Korkmaz
D058186:Acute Kidney Injury / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D007668:Kidney / Q000473:pathology; D008297:Male; D010126:Ozone / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000473:pathology / Q000517:prevention & control*
D010126:Ozone