Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.
We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak interactions. Conversion of ligands into SNAP-tag substrates thus provides access to a powerful toolbox for the analysis of their interactions with proteins.
École Polytechnique Fédérale de Lausanne-EPFL, Institute of Chemical Sciences and Engineering, Institute of Bioengineering, National Centre of Competence in Research-NCCR in Chemical Biology, Lausanne, Switzerland.
Hirohito
H
Haruki
Monica Rengifo
MR
Gonzalez
Kai
K
Johnsson
D008024:Ligands; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
D008024:Ligands; D011506:Proteins; D011993:Recombinant Fusion Proteins