Recognition of Hg2+ ion through restricted imine isomerization: crystallographic evidence and imaging in live cells.
A newly synthesized imine-based receptor (L) showed remarkable specificity toward the Hg(2+) ion in aqueous media over other metal ions. Coordination of L to Hg(2+) induces a turn-on fluorescence response. This was explained based on the restricted imine isomerization along with PET on coordination to Hg(2+). X-ray structural evidence tends to favor a C-C bond rotation rather than C═N isomerization for adopting a favorable conformation in L for coordination to Hg(2+). This reagent could be used for imaging the accumulation of Hg(2+) ions in HeLa cells.
Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar 364002, Gujarat, India.
Amal Kumar
AK
Mandal
Moorthy
M
Suresh
Priyadip
P
Das
E
E
Suresh
Mithu
M
Baidya
Sudip K
SK
Ghosh
Amitava
A
Das
D002413:Cations, Divalent / Q000737:chemistry; D002470:Cell Survival; D018360:Crystallography, X-Ray; D006367:HeLa Cells; D006801:Humans; D007097:Imines / Q000737:chemistry*; D007536:Isomerism; D008628:Mercury / Q000032:analysis* / Q000737:chemistry
D002413:Cations, Divalent; D007097:Imines; D008628:Mercury