PARP16/ARTD15 is a novel endoplasmic-reticulum-associated mono-ADP-ribosyltransferase that interacts with, and modifies karyopherin-ß1.
BACKGROUND
Protein mono-ADP-ribosylation is a reversible post-translational modification that modulates the function of target proteins. The enzymes that catalyze this reaction in mammalian cells are either bacterial pathogenic toxins or endogenous cellular ADP-ribosyltransferases. The latter include members of three different families of proteins: the well characterized arginine-specific ecto-enzymes ARTCs, two sirtuins and, more recently, novel members of the poly(ADP-ribose) polymerase (PARP/ARTD) family that have been suggested to act as cellular mono-ADP-ribosyltransferases. Here, we report on the characterisation of human ARTD15, the only known ARTD family member with a putative C-terminal transmembrane domain.


RESULTS
Immunofluorescence and electron microscopy were performed to characterise the sub-cellular localisation of ARTD15, which was found to be associated with membranes of the nuclear envelope and endoplasmic reticulum. The orientation of ARTD15 was determined using protease protection assay, and is shown to be a tail-anchored protein with a cytosolic catalytic domain. Importantly, by combining immunoprecipitation with mass spectrometry and using cell lysates from cells over-expressing FLAG-ARTD15, we have identified karyopherin-ß1, a component of the nuclear trafficking machinery, as a molecular partner of ARTD15. Finally, we demonstrate that ARTD15 is a mono-ADP-ribosyltransferase able to induce the ADP-ribosylation of karyopherin-ß1, thus defining the first substrate for this enzyme.


CONCLUSIONS
Our data reveal that ARTD15 is a novel ADP-ribosyltransferase enzyme with a new intracellular location. Finally, the identification of karyopherin-ß1 as a target of ARTD15-mediated ADP-ribosylation, hints at a novel regulatory mechanism of karyopherin-ß1 functions.
Consorzio Mario Negri Sud, Santa Maria Imbaro (Chieti), Italy.
Simone
S
Di Paola
Massimo
M
Micaroni
Giuseppe
G
Di Tullio
Roberto
R
Buccione
Maria
M
Di Girolamo
D036002:ADP Ribose Transferases / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000201:enzymology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D013058:Mass Spectrometry; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000201:enzymology* / Q000648:ultrastructure; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D028961:beta Karyopherins / Q000378:metabolism*
D028961:beta Karyopherins; D036002:ADP Ribose Transferases; C576117:PARP16 protein, human; D011065:Poly(ADP-ribose) Polymerases