[Tissue distribution of 1,3-diphenyl-1,3-propanedione in mice].
OBJECTIVE
To investigate tissue distribution characteristics of 1,3-diphenyl-1,3-propanedione (DPPD) in mice.


METHODS
Male ICR mice were  dosed with DPPD 500 mg/kg via oral gavage, and the tissue samples of the heart, liver, spleen, lungs, kidneys and muscle of each mouse were collected  as scheduled. At each time point, the concentrations of DPPD in the mouse tissues were measured by high performance liquid chromatography (HPLC)  method. The main pharmacokinetic parameters were calculated by Thermo Kinetica 4.4.1 software.


RESULTS
DPPD was absorbed rapidly after oral  administration. The concentrations of DPPD in the liver and in the kidney were higher, respectively (liver: AUC(tot)=41.92 μg×h/g, kidney: AUC(tot)=40.40 μg×h/g). The drug concentrations showed a rapid distribution in the liver and lungs (T(max)=0.32 h and 0.33 h respectively) after oral administration, but in the  muscle the maximum was 3.85 h. The maximum concentration of DPPD was in the liver (C(max)=31.20 μg/g), which was also the highest tissue concentration  of all the subjects. DPPD could be detected at the low concentration within 24 h in all the tissues involved.


CONCLUSIONS
DPPD distributed unevenly in  various tissues. In the liver, kidney and muscle, the amount of the drug concentration was larger, and was lower in the lungs and spleen.
Beijing Centers for Preventive Medical Research, Beijing, China.
Han-mei
HM
Tian
Rong
R
Cui
Yue-ying
YY
He
Yan
Y
Lv
Bao-xue
BX
Zhang
D000818:Animals; D047188:Chalcones / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D014018:Tissue Distribution
D047188:Chalcones; C061481:dibenzoylmethane