Improving the detection of fungi in eosinophilic mucin: seeing what we could not see before.
OBJECTIVE
To investigate the improvement in histologic detection of fungi with Gomori methenamine silver (GMS) stain by trypsin predigestion in the mucus of patients with chronic rhinosinusitis (CRS).


METHODS
Prospective, single group, descriptive analysis.


METHODS
Multi-institutional.


METHODS
Thirty-four sinus specimens from 12 surgical patients with CRS were stained with hematoxylin and eosin, GMS stain, GMS with trypsin digestion, immunofluorescence stains for chitinase, and anti-Alternaria. All patients received skin testing, total IgE serology, and radioallergosorbent tests (RAST) for 23 fungal-specific IgE antibodies.


RESULTS
The conventional GMS stain detected fungi in only 9 of 34 (27%) specimens. Predigesting the specimen with trypsin dramatically improved the visualization of fungi (31/34, 91%). The chitinase immunofluorescence visualized fungi in 32 of 34 (94%), and anti-Alternaria visualized 33 of 34 specimens (97%). Only 8 of 12 (75%) patients had detectable allergies.


CONCLUSIONS
This report describes a simple modification of the conventional GMS stain that can significantly improve the visualization of fungi on histology and explains the lack of detection in previous studies. These novel, more sensitive histologic methods reveal the presence of fungi within the eosinophilic mucin in allergic and also nonallergic CRS patients, further questioning a crucial role of an IgE-mediated pathophysiology.
University at Buffalo School of Medicine and Biomedical Sciences, Buffalo, NY, USA. cantingg@buffalo.edu
Canting
C
Guo
Saied
S
Ghadersohi
Gail M
GM
Kephart
Roger A
RA
Laine
David A
DA
Sherris
Hirohita
H
Kita
Jens U
JU
Ponikau
D000328:Adult; D000368:Aged; D002908:Chronic Disease; D004396:Coloring Agents / Q000737:chemistry*; D004804:Eosinophils; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D008709:Methenamine; D008875:Middle Aged; D009077:Mucins; D009093:Mucus / Q000382:microbiology*; D011446:Prospective Studies; D012220:Rhinitis / Q000382:microbiology*; D012852:Sinusitis / Q000382:microbiology*; D013194:Staining and Labeling
D004396:Coloring Agents; D009077:Mucins; D008709:Methenamine