Simultaneous determination of baicalin, wogonoside, baicalein, wogonin, oroxylin A and chrysin of Radix scutellariae extract in rat plasma by liquid chromatography tandem mass spectrometry.
A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of baicalin, wogonoside, baicalein, wogonin, oroxylin A and chrysin in rat plasma, using naringin as an internal standard. After acidifying with HCl, plasma samples were pretreated by liquid-liquid extraction with acetone. Chromatographic separation was accomplished on a Hypersil Gold-C(18) analytical column (2.1×150 mm, 5 μm) utilizing a gradient elution profile and a mobile phase consisting of (A) 0.1% formic acid in water and (B) acetonitrile. Detection was performed by multiple reaction monitoring (MRM) mode using electrospray ionization in the positive ion mode. All analytes showed good linearity over the investigated concentration range (r>0.9900). The lower limit of quantification was 0.5 ng/ml for baicalin, wogonoside, wogonin and oroxylin A, and 1.0 ng/ml for baicalein and chrysin. Intra-day and inter-day precisions (RSD%) were less than 15% and accuracy (RE%) ranged from -6.7% to 5.8%. The validated method was successfully applied to investigate the pharmacokinetics of the major flavonoids of Radix scutellariae extract after oral administration to rats.
School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China.
Ling
L
Tong
Meixu
M
Wan
Lihua
L
Zhang
Yonghong
Y
Zhu
He
H
Sun
Kaishun
K
Bi
D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D044950:Flavanones / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D005419:Flavonoids / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D005960:Glucosides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D006207:Half-Life; D057230:Limit of Detection; D008297:Male; D008657:Metabolic Clearance Rate; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results; D031364:Scutellaria baicalensis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000592:standards
D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D010936:Plant Extracts; C038044:baicalin; C043561:chrysin; C006680:baicalein; C080669:5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one; C473995:wogonoside; C085514:wogonin