Profundibacterium mesophilum gen. nov., sp. nov., a novel member in the family Rhodobacteraceae isolated from deep-sea sediment in the Red Sea, Saudi Arabia.
A slow-growing, strictly aerobic, Gram-negative, coccus bacterial strain, designated KAUST100406-0324(T), was isolated from sea-floor sediment collected from the Red Sea, Saudi Arabia. The catalase- and oxidase-positive strain was non-sporulating and only slightly halophilic. Optimum growth occurred at 20-25 °C and at pH values ranging from 7.0 to 8.0. The major cellular fatty acids of the strain were unsaturated C18 : 1ω6c and/or C18 : 1ω7c, C18 : 1ω7c 11-methyl and C16 : 1ω7c and/or C16 : 1ω6c. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. Ubiquinone 10 was the predominant lipoquinone. The DNA G+C content of strain KAUST100406-0324(T) was 64.0 mol%. Phylogenetic analysis of 16S rRNA gene sequences revealed that the novel strain belonged to the family Rhodobacteraceae of the class Alphaproteobacteria but formed a distinct evolutionary lineage from other bacterial species with validly published names. The 16S rRNA gene sequence of the novel strain was distantly related, but formed a monophyletic cluster with, those of bacteria from two moderately halophilic genera, Hwanghaeicola and Maribius. The similarity of the sequence between the novel strain KAUST100406-0324(T) and the type strains Hwanghaeicola aestuarii Y26(T) (accession number FJ230842), Maribius pelagius B5-6(T) (DQ514326) and Maribius salinus CL-SP27(T) (AY906863) were 94.5 %, 95.2 % and 95.3 %, respectively. Based on the physiological, phylogenetic and chemotaxonomic characteristics presented in this study, we propose that this strain represents a novel species of a new genus in the family Rhodobacteraceae, for which the name of Profundibacterium mesophilum gen. nov., sp. nov. was proposed, with KAUST100406-0324(T) ( = JCM 17872(T)  = NRRL B-59665(T)) as the type strain.
KAUST Global Collaborative Research Program, Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
Pok Yui
PY
Lai
Institute of Marine Science and Technology, Yangzhou University. No. 196, West Huayang Street, Yangzhou City, Jiangsu Province, PR China.
Li
L
Miao
KAUST Global Collaborative Research Program, Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
On On
OO
Lee
KAUST Global Collaborative Research Program, Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
Ling-Li
LL
Liu
Institute of Marine Science and Technology, Yangzhou University. No. 196, West Huayang Street, Yangzhou City, Jiangsu Province, PR China.
Xiao-Jian
XJ
Zhou
KAUST Global Collaborative Research Program, Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
Ying
Y
Xu
The King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Abdulaziz
A
Al-Suwailem
KAUST Global Collaborative Research Program, Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
Pei-Yuan
PY
Qian
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D019156:Indian Ocean; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012529:Saudi Arabia; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2