Nucleosome dynamics between tension-induced states.
We studied the dynamical behavior of a mononucleosome under tension using a theoretical model that takes into account the nucleosomal geometry, DNA elasticity, nonspecific DNA-protein binding, and effective repulsion between the two DNA turns. Using a dynamical Monte-Carlo simulation algorithm, we demonstrate that this model shows a behavior that for an appropriate set of parameters is in quantitative agreement with data from micromanipulation experiments on individual nucleosomes. All of the parameters of the model follow from the data obtained from two types of pulling experiments, namely, constant force and constant loading rate ensembles.
Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences, Zanjan, Iran. laleh@iasbs.ac.ir
Laleh
L
Mollazadeh-Beidokhti
Farshid
F
Mohammad-Rafiee
Helmut
H
Schiessel
D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism*; D013314:Stress, Mechanical; D013816:Thermodynamics; D013997:Time Factors
D009707:Nucleosomes; D004247:DNA