Gene expression profiling of hybridoma cells after bursal-derived bioactive factor BP5 treatment.
Bursa of Fabricius is the acknowledged vital humoral immune system for B cell differentiation and antibody production. To study the molecular mechanism underlying the effect of bursal-derived BP5, we used gene microarray to analyze the genomic expression profiling of BP5-treated hybridoma cells. BP5 exhibited an immunomodulatory effect on antibody production in hybridoma cells and induced alterations in the gene expression profiles related to the immune-related biological processes, such as T cell activation and proliferation, B cell activation, B cell-mediated immunity, and cytokines cytokine production involved in immune response. In addition, 26 biological pathways associated with immunomodulatory functions were regulated in BP5-treated hybridoma cells, in which p53 signal pathway played an important role in antitumor. Among these regulated genes, 12 differentially expressed genes were verified by qRT-PCR. The activation of p53 activity by BP5 was further confirmed by p53 luciferase reporter assay and p53 expression. Our data revealed that bursal-derived BP5 could regulate various immune-related cellular processes, including antitumor factor p53 signal pathway, perhaps partially accounting for the reported immunomodulatory roles and novel antiproliferation on tumor cells functions of bursal-derived bioactive factor BP5.
Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, College of Veterinary Medicine, Nanjing Agriculture University, Nanjing, 210095, China.
Xiu L
XL
Feng
Qing T
QT
Liu
Rui B
RB
Cao
Bin
B
Zhou
De Y
de Y
Li
Yuan P
YP
Zhang
Ke
K
Liu
Xiao D
XD
Liu
Jian C
JC
Wei
Ya F
YF
Qiu
Xin F
XF
Li
Zhi Y
ZY
Ma
Pu Y
PY
Chen
D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004354:Drug Screening Assays, Antitumor; D020869:Gene Expression Profiling; D006367:HeLa Cells; D006801:Humans; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013329:Structure-Activity Relationship; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014709:Vero Cells
D000970:Antineoplastic Agents; D010455:Peptides; D016159:Tumor Suppressor Protein p53