Rapid, accurate particle tracking by calculation of radial symmetry centers.
I introduce an algorithm for subpixel localization of imaged objects based on an analytic, non-iterative calculation of the best-fit radial symmetry center. This approach yields tracking accuracies that are near theoretical limits, similarly to Gaussian fitting, but with orders-of-magnitude faster execution time, lower sensitivity to nearby particles and applicability to any radially symmetric intensity distribution. I demonstrate the method with several types of data, including super-resolution microscopy images.
Department of Physics, University of Oregon, Eugene, Oregon, USA. raghu@uoregon.edu
Raghuveer
R
Parthasarathy
D000465:Algorithms; D003198:Computer Simulation; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008962:Models, Theoretical; D010316:Particle Size; D012680:Sensitivity and Specificity; D013997:Time Factors