Allosteric activation and contrasting properties of L-serine dehydratase types 1 and 2.
Bacterial L-serine dehydratases differ from mammalian L- and D-serine dehydratases and bacterial D-serine dehydratases by the presence of an iron-sulfur center rather than a pyridoxyl phosphate prosthetic group. They exist in two forms, types 1 and 2, distinguished by their sequence and oligomeric configuration. Both types contain an ASB domain, and the type 1 enzymes also contain an ACT domain in a tandem arrangement with the ASB domain like that in type 1 D-3-phosphoglycerate dehydrogenases (PGDHs). This investigation reveals striking kinetic differences between L-serine dehydratases from Bacillus subtilis (bsLSD, type 1) and Legionella pneumophila (lpLSD, type 2). lpLSD is activated by monovalent cations and inhibited by monovalent anions. bsLSD is strongly activated by cations, particularly potassium, and shows a mixed response to anions. Flouride is a competitive inhibitor for lpLSD but an apparent activator for bsLSD at low concentrations and an inhibitor at high concentrations. The reaction products, pyruvate and ammonia, also act as activators but to different extents for each type. Pyruvate activation is competitive with L-serine, but activation of the enzyme is not compatible with it simply competing for binding at the active site and suggests the presence of a second, allosteric site. Because activation can be eliminated by higher levels of L-serine, it may be that this second site is actually a second serine binding site. This is consistent with type 1 PGDH in which the ASB domain functions as a second site for substrate binding and activation.
Department of Developmental Biology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8103, St. Louis, MO 63110, USA.
Shawei
S
Chen
Xiao Lan
XL
Xu
Gregory A
GA
Grant
D000494:Allosteric Regulation; D001412:Bacillus subtilis / Q000201:enzymology; D012695:L-Serine Dehydratase / Q000378:metabolism*; D016952:Legionella pneumophila / Q000201:enzymology; D008954:Models, Biological
D012695:L-Serine Dehydratase