Islet isolation and purification from inbred Wuzhishan miniature pigs.
BACKGROUND
To investigate the applicability of inbred Wuzhishan (WZS) miniature pigs for porcine islet isolation and purification.


METHODS
Islet isolation and purification was conducted on adult (1-yr-old), male inbred WZS miniature pigs and age- and sex-matched market pigs obtained from a local slaughterhouse (control group). Pancreata were excised, and islet isolation was carried out by static digestion and discontinuous gradient centrifugation. Viability of the purified islets was tested by radioimmunochemistry assay to measure glucose-induced insulin release in culture and transplantation in an in vivo study.


RESULTS
The anatomical structure of the WZS miniature pig pancreas was more similar to the human pancreas than that of the market pig. Islet yield of the WZS miniature pigs' pancreata was significantly higher than that of the market pigs (6078 ± 1105 vs. 2500 ± 625 islet equivalents [IEQ]/g). In vitro study demonstrated that the islets isolated from WZS miniature pigs were viable, as they efficiently responded to glucose challenge. In vivo study showed that the islets from both groups could cure the diabetic rat with the survival varied from 3 to 5 days (median, 4.3 days) and 2-4 days (median, 3.6 days) in experimental group and control group, respectively.


CONCLUSIONS
Wuzhishan miniature pig pancreas may be a feasible source of islets for xenotransplantation.
Department of Surgery, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Xiaofeng
X
Jiang
Taolai
T
Qian
Thomas
T
Linn
Liangqi
L
Cao
Guangyang
G
Xiang
Yezeng
Y
Wang
Heping
H
Peng
Ping
P
Xue
Lehong
L
Zhang
De
D
Chen
Xuewei
X
Yang
D000818:Animals; D001947:Breeding; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008099:Liver / Q000601:surgery; D008297:Male; D009924:Organ Culture Techniques; D009929:Organ Size; D013045:Species Specificity; D034421:Sus scrofa; D013552:Swine; D013556:Swine, Miniature; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014183:Transplantation, Heterologous / Q000379:methods*
D007328:Insulin; D005947:Glucose