Recombinant Shiga toxin B subunit can induce neutralizing immunoglobulin Y antibody.
Previously, we have shown that chickens immunized with Shiga toxin (Stx) produce Stx-neutralizing egg yolk immunoglobulin Y (IgY) antibody. The anti-Stx-1 IgY and anti-Stx-2 IgY exert their neutralizing activity through their antibody activity against the B subunit of the toxin but not the A subunit. In the present study, chickens were immunized with recombinant Stx-1 B subunit (rStx-1B) and recombinant Stx-2 B subunit (rStx-2B). Induced anti-rStx-1B and anti-rStx-2B IgY neutralized the toxicity of Stx-1 and Stx-2 against HeLa 229 cells. The neutralizing activity of anti-rStx-1B IgY on Stx-1 was almost 10 times stronger than that of anti-Stx-1 IgY, and that of anti-rStx-2B IgY was 2.6 times stronger than that of anti-Stx-2 IgY. Anti-rStx-1B and anti-rStx-2B IgY reacted with multimeric and monomeric forms of the B subunits in contrast to anti-Stx-1 and anti-Stx-2 IgY that reacted with only the multimeric form. These results indicated that recombinant B subunits were promising antigens for induction of neutralizing antibodies in chickens.
Microbiology, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University, Japan.
Paola
P
Neri
Shunji
S
Tokoro
Tsuyoshi
T
Sugiyama
Kouji
K
Umeda
Takeshi
T
Shimizu
Takao
T
Tsuji
Yoshikatsu
Y
Kodama
Hiroshi
H
Mori
D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D002645:Chickens; D004530:Egg Yolk / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D007136:Immunoglobulins / Q000276:immunology*; D021122:Protein Subunits / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D022621:Shiga Toxin / Q000276:immunology*
D057134:Antibodies, Neutralizing; C016512:IgY; D007136:Immunoglobulins; D021122:Protein Subunits; D011994:Recombinant Proteins; D022621:Shiga Toxin