Obstructed diffusion propagator analysis for single-particle tracking.
We describe a method for the analysis of the distribution of displacements, i.e., the propagators, of single-particle tracking measurements for the case of obstructed subdiffusion in two-dimensional membranes. The propagator for the percolation cluster is compared with a two-component mobility model against Monte Carlo simulations. To account for diffusion in the presence of obstacle concentrations below the percolation threshold, a propagator that includes the transient motion in finite percolation clusters and hopping between obstacle-induced compartments is derived. Finally, these models are shown to be effective in the analysis of Kv2.1 channel diffusive measurements in the membrane of living mammalian cells.
School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado 80523, USA.
Aubrey V
AV
Weigel
Shankarachary
S
Ragi
Michael L
ML
Reid
Edwin K P
EK
Chong
Michael M
MM
Tamkun
Diego
D
Krapf
D004867:Equipment Design; D019544:Equipment Failure Analysis; D057054:Molecular Imaging / Q000295:instrumentation*; D009038:Motion; D053758:Nanoparticles / Q000648:ultrastructure*; D012031:Refractometry / Q000295:instrumentation*