Discrimination between glycosylation patterns of therapeutic antibodies using a microfluidic platform, MALDI-MS and multivariate statistics.
Optimal glycosylation with respect to the efficacy, serum half-life time, and immunogenic properties is essential in the generation of therapeutic antibodies. The glycosylation pattern can be affected by several different parameters during the manufacture of antibodies and may change significantly over cultivation time. Fast and robust methods for determination of the glycosylation patterns of therapeutic antibodies are therefore needed. We have recently presented an efficient method for the determination of glycans on therapeutic antibodies using a microfluidic CD platform for sample preparation prior to matrix-assisted laser-desorption mass spectrometry analysis. In the present work, this method is applied to analyse the glycosylation patterns of three commercially available therapeutic antibodies and one intended for therapeutic use. Two of the antibodies produced in mouse myeloma cell line (SP2/0) and one produced in Chinese hamster ovary (CHO) cells exhibited similar glycosylation patterns but could still be readily differentiated from each other using multivariate statistical methods. The two antibodies with most similar glycosylation patterns were also studied in an assessment of the method's applicability for quality control of therapeutic antibodies. The method presented in this paper is highly automated and rapid. It can therefore efficiently generate data that helps to keep a production process within the desired design space or assess that an identical product is being produced after changes to the process.
Department of Analytical Chemistry, Stockholm University, SE-10691 Stockholm, Sweden.
Tran Thi
TT
Thuy
Erik
E
Tengstrand
Magnus
M
Aberg
Gunnar
G
Thorsén
D000818:Animals; D000906:Antibodies / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use; D001331:Automation; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D016002:Discriminant Analysis; D006031:Glycosylation; D051379:Mice; D046210:Microfluidic Analytical Techniques; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D011499:Protein Processing, Post-Translational; D011786:Quality Control; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism* / Q000592:standards / Q000627:therapeutic use; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014162:Transfection; D057188:Workflow
D000906:Antibodies; D011994:Recombinant Proteins