Production of isomaltooligosaccharides from banana flour.
BACKGROUND
Banana is one of the important crops native to tropical Southeast Asia. Since overproduction frequently leads to excessive waste of produce, alternative uses are continuously sought in order to utilise fruits at all stages of maturity. The aim of this study was to investigate the production of isomaltooligosaccharides (IMOs) from banana flour.


RESULTS
Banana slurries liquefied by Termamyl SC and saccharified by either Fungamyl 800 L or barley β-amylase were used for IMO synthesis by Transglucosidase L. After 12 h of transglucosylation, maximum IMO yields of 76.67 ± 2.71 and 70.74 ± 4.09 g L(-1) respectively were achieved. Although the yields were comparable, the IMO profiles obtained through the use of the two saccharification enzymes were different. Glucose and maltose were removed by 10 g L(-1) bakers' yeast fermentation for 12 h. Regarding total sugars, the final IMO mixture was composed of 53% isomaltotriose, 21% isomaltotetraose and 26% maltooligoheptaose and larger oligomers.


CONCLUSIONS
Banana flour could be used as a potential raw material for IMO synthesis.
Department of Applied Sciences, Faculty of Science and Technology, Loei Rajabhat University, Loei 42000, Thailand.
Suwimol
S
Chockchaisawasdee
Naiyatat
N
Poosaran
D005285:Fermentation; D005433:Flour; D005638:Fruit; D005947:Glucose / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism*; D001467:Hordeum / Q000201:enzymology; D007534:Isomaltose / Q000096:biosynthesis*; D008320:Maltose / Q000378:metabolism; D028521:Musa; D009844:Oligosaccharides / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae; D014312:Trisaccharides / Q000096:biosynthesis; D000516:alpha-Amylases / Q000378:metabolism; D001614:beta-Amylase / Q000378:metabolism
D009844:Oligosaccharides; D014312:Trisaccharides; C045441:isomaltotriose; C047512:isomaltotetraose; D007534:Isomaltose; D008320:Maltose; D005959:Glucosidases; D000516:alpha-Amylases; C582722:termamyl; D001614:beta-Amylase; D005947:Glucose