Anthocyanin identification and composition of wild Vitis spp. accessions by using LC-MS and LC-NMR.
The composition and concentration of anthocyanins of grape berry skins were analyzed in order to assess phenotypic variation between four grape wine varieties belonging to 4 different species: Vitis vinifera, Vitis amurensis, Vitis cinerea and Vitis X champinii. High-performance liquid chromatography coupled to mass spectrometry (LC-MS) and NMR spectroscopy (LC-NMR) were used to separate and identify the structure of anthocyanins present in these species. Combination of LC-MS and LC-NMR data resulted in the identification of 33 anthocyanins. In particular, newly reported cis isomers of p-coumaric-derivatives were identified (petunidin-, peonidin- and malvidin-3-(6-p-coumaroyl)-5-diglucoside). In V. cinerea and V. vinifera, anthocyanins were monoglucoside derivatives whereas in V. amurensis and V. X champinii, both mono- and diglucoside derivatives were identified. Malvidin-, delphinidin- and petunidin-derivatives were, respectively, the most abundant components in V. cinerea and V. vinifera, V. amurensis and V. X champinii.
Université de Bordeaux, ISVV, EA 3675 GESVAB, 33882 Villenave d'Ornon, France.
Alexander
A
Acevedo De la Cruz
Ghislaine
G
Hilbert
Céline
C
Rivière
Virginie
V
Mengin
Nathalie
N
Ollat
Louis
L
Bordenave
Stéphane
S
Decroocq
Jean-Claude
JC
Delaunay
Serge
S
Delrot
Jean-Michel
JM
Mérillon
Jean-Pierre
JP
Monti
Eric
E
Gomès
Tristan
T
Richard
D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D021241:Spectrometry, Mass, Electrospray Ionization; D027843:Vitis / Q000737:chemistry*
D000872:Anthocyanins; D005960:Glucosides