Fast multispectral diffuse optical tomography system for in vivo three-dimensional imaging of seizure dynamics.
We describe a multispectral continuous-wave diffuse optical tomography (DOT) system that can be used for in vivo three-dimensional (3-D) imaging of seizure dynamics. Fast 3-D data acquisition is realized through a time multiplexing approach based on a parallel lighting configuration--our system can achieve 0.12 ms per source per wavelength and up to a 14 Hz sampling rate for a full set of data for 3-D DOT image reconstruction. The system is validated using both static and dynamic tissue-like phantoms. An initial in vivo experiment using a rat model of seizure is also demonstrated.
Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA.
Jianjun
J
Yang
Tao
T
Zhang
Hao
H
Yang
Huabei
H
Jiang
D000818:Animals; D001640:Bicuculline / Q000008:administration & dosage / Q000031:analogs & derivatives; D002138:Calibration; D004058:Diffusion; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D008297:Male; D019047:Phantoms, Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology*; D013053:Spectrophotometry; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods
C017069:bicuculline methiodide; D001640:Bicuculline