Oral and genital human herpesvirus 8 and human papillomavirus in heterosexual partners.
BACKGROUND
The purpose of this study was to verify a possible co-infection of human herpesvirus 8 (HHV-8) in commonly associated human papillomavirus (HPV) penile lesions and to determine the frequency of detection of these viruses in the oral mucosa of their female counterparts.


METHODS
Thirty-one male subjects underwent penile swabs from clinical HPV-related lesions. Their female counterparts underwent swabs of the vagina, uterine cervix, and oral mucosa. HPV and HHV-8 detection was performed by polymerase chain reaction using the consensus primers MY11/MY09 and KS1/KS2, respectively.


RESULTS
HPV DNA was detected in 31/31 penile lesions. HPV DNA was also detected in 18/31 (58%) female genital brushings and 17/31 (54%) female oral brushings. HHV-8 DNA was detected in 1/31 (3.2%) male genital brushings and 3/31 (9.6%) female oral mucosa brushings. None of the female genital brushings were HHV-8 DNA-infected.


CONCLUSIONS
Based upon the results of this study, co-infection between HPV and HHV-8 in malignant and pre-malignant penile lesions is an unlikely finding.
Departmento de Clínica e Odontologia Preventiva, School of Dentistry, Oral Medicine Unit, Universidade Federal de Pernambuco/UFPE, Recife, PE, Brazil.
Catarina da Mota Vasconcelos
Cda M
Brasil
Camila Maria Beder
CM
Ribeiro
Jair Carneiro
JC
Leão
D000293:Adolescent; D000328:Adult; D052685:Alphapapillomavirus / Q000302:isolation & purification; D002294:Carcinoma, Squamous Cell / Q000821:virology; D016009:Chi-Square Distribution; D060085:Coinfection / Q000821:virology*; D005260:Female; D005836:Genitalia, Female / Q000821:virology*; D006566:Herpesviridae Infections / Q000635:transmission*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification; D020010:Heterosexuality; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000821:virology*; D030361:Papillomavirus Infections / Q000635:transmission* / Q000821:virology; D010412:Penile Neoplasms / Q000821:virology*; D012514:Sarcoma, Kaposi / Q000821:virology; D012747:Sexual Partners; D011795:Surveys and Questionnaires; D017201:Virus Shedding; D055815:Young Adult