Chiral pharmacokinetics of zaltoprofen in rats by HPLC with solid-phase extraction.
We investigated the pharmacokinetic profile of (R)- and (S)-zaltoprofen (ZPF) in rats using rapid and selective liquid chromatography with solid-phase extraction (SPE). The ZPF enantiomers were extracted from a small volume of plasma (0.2 mL) by means of SPE using cartridges and were analyzed on a Chiralcel OJ-H (4.6 mm × 150 mm, 5 μm) column with ultraviolet detection at 244 nm. The lower limit of quantification of the ZPF enantiomers in plasma was 0.1μg/mL. The validated method was successfully applied to chiral pharmacokinetic studies of oral administration of racemic ZPF to rats. (S)-ZPF showed significantly higher AUC, T(max), and C(max) and a longer half-life than (R)-ZPF, indicating higher bioavailability of the (S)-isomer. A total of 8 samples (about 12% of the total number of samples) were selected for incurred sample reanalysis (ISR). The % difference between the re-assay concentrations and the original concentrations were all less than 15% of their mean values and met the acceptance criteria for ISR.
College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
Van Men
VM
Chu
Kyung Tae
KT
Kim
Sang Huyck
SH
Kim
Wonjae
W
Lee
Ki Cheul
KC
Lee
Van Long
VL
Nguyen
Van Luong
VL
Hoang
Young Keun
YK
Lee
Kyung Rae
KR
Park
San Hun
SH
Jung
Jong Seong
JS
Kang
D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001578:Benzopyrans / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D001682:Biological Availability; D002138:Calibration; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D004355:Drug Stability; D006207:Half-Life; D007536:Isomerism; D057230:Limit of Detection; D016014:Linear Models; D008657:Metabolic Clearance Rate; D011422:Propionates / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000592:standards; D013056:Spectrophotometry, Ultraviolet
D001578:Benzopyrans; D011422:Propionates; C043940:pyranoprofen; D002482:Cellulose