Chemical composition of currant seed extracts and their protective effect on human lymphocytes DNA.
On the basis of LC/UV/MS analysis, 35 compounds from the extracts of seeds of black, red, and white currants (2 cultivars of each) were identified. Black currants cultivars contained protocatechuic acid and p-hydroxybenzoic acid, and traces of nitril containing phenolic acids. The presence of synapoyl glucoside was characteristic for cv. Malling Jewel. Sesquiterpenoid glycosides and carboxymethylindol glycosides were present mainly in white and red currant cultivars. Blackcurrant seeds contained higher amounts of flavonoids, especially rutin, isoquercetin, and taxifolin. The currant seed extracts were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. The frequency of MN was scored in binucleated cells, and the cultivars of black currants showed the best antioxidant potential. At a concentration of only 0.17 mg/mL, extract of the seed cv. Malling Jewel effected a decrease in the frequency of MN of 60% compared with control cell cultures.


CONCLUSIONS
Our results provide evidence of protective effects of currant seed extracts and isolated pure compounds on cytogenetic damages in human lymphocytes. Thus, currant seed extracts could exert beneficial effects in quite a few diseases, for many of the biological actions have been attributed to their antioxidant properties.
Inst of Chemistry, Technology and Metallurgy, Njegoševa 12, Univ of Belgrade, Serbia. dgodjev@chem.bg.ac.rs
Dejan
D
Gođevac
Vele
V
Tešević
Vlatka
V
Vajs
Slobodan
S
Milosavljević
Gordana
G
Zdunić
Boban
B
Dorđević
Miroslava
M
Stanković
D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D062385:Hydroxybenzoates / Q000032:analysis / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D015162:Micronucleus Tests; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D031965:Ribes / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
D000975:Antioxidants; D005419:Flavonoids; D062385:Hydroxybenzoates; D010936:Plant Extracts; C009091:protocatechuic acid; D004247:DNA; C017616:phenolic acid