1β-Hydroxy-2-oxopomolic acid isolated from Agrimonia pilosa extract inhibits adipogenesis in 3T3-L1 cells.
In order to determine anti-adipogenic effect, this study investigated 1β-hydroxy-2-oxopomolic acid (HOA) isolated from Agrimonia pilosa inhibits adipocyte differentiation and expression of adipogenic marker genes, such as peroxisome proliferator activated receptor γ (PPARγ), CCAAT-enhancer-binding protein α (C/EBPα), glucose transporter 4 (GLUT4), adiponectin, adipocyte fatty acid-binding protein 2 (aP2), adipocyte determination and differentiation factor 1/sterol regulatory element binding protein 1c (ADD1/SREBP1c), resistin, and fatty acid synthase (Fas) in 3T3-L1 preadipocyte. We demonstrated that HOA induced a significant decrease in lipid accumulation and expression of adipogenic marker genes in a dose-dependent manner. In addition, HOA reduced the transcripitional activity of PPARγ induced by troglitazone, a potent diabetes agent; it also suppressed expression of PPARγ and C/EBPα protein levels. Our data suggest that HOA isolated from Agrimonia pilosa inhibits adipocyte differentiation through downregulation of various adipocytokines by blocking PPARγ and C/EBPα expression.
Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion, Suwon-si, Gyeonggi-do, Republic of Korea.
Eun-Kyung
EK
Ahn
Jung A
JA
Lee
Dong-Wan
DW
Seo
Seong Su
SS
Hong
Joa Sub
JS
Oh
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D054392:Adipokines / Q000235:genetics / Q000378:metabolism*; D031981:Agrimonia / Q000737:chemistry*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D002839:Chromans / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D015870:Gene Expression / Q000187:drug effects; D005819:Genetic Markers; D007004:Hypoglycemic Agents / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D045162:Thiazolidinediones / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D000077288:Troglitazone
C412808:2-oxopomolic acid; D054392:Adipokines; D022763:CCAAT-Enhancer-Binding Protein-alpha; D002839:Chromans; D005819:Genetic Markers; D007004:Hypoglycemic Agents; D047495:PPAR gamma; D010936:Plant Extracts; D045162:Thiazolidinediones; D014315:Triterpenes; D000077288:Troglitazone