Development of a diagnostic method applicable to various serotypes of hantavirus infection in rodents.
Antigenic diversity among different hantaviruses requires a variety of reagents for diagnosis of hantavirus infection. To develop a diagnostic method applicable to various hantavirus infections with a single set of reagents, we developed an enzyme-linked immunosorbent assay (ELISA) using recombinant nucleocapsid proteins of three hantaviruses, Amur, Hokkaido, and Sin Nombre viruses. This novel cocktail antigen-based ELISA enabled detection of antibodies against Hantaan, Seoul, Amur, Puumala, and Sin Nombre viruses in immunized laboratory animals. In wild rodent species, including Apodemus, Rattus, and Myodes, our ELISA detected antibodies against hantaviruses with high sensitivity and specificity. These data suggest that our novel diagnostic ELISA is a useful tool for screening hantavirus infections and could be effectively utilized for serological surveillance and quarantine purposes.
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18 Nishi-9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
Takahiro
T
Sanada
Hiroaki
H
Kariwa
Ngonda
N
Saasa
Keisuke
K
Yoshikawa
Takahiro
T
Seto
Vyacheslav G
VG
Morozov
Evgeniy A
EA
Tkachenko
Leonid I
LI
Ivanov
Kumiko
K
Yoshimatsu
Jiro
J
Arikawa
Kentaro
K
Yoshii
Ikuo
I
Takashima
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D003411:Arvicolinae; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D006476:Orthohantavirus / Q000235:genetics*; D018778:Hantavirus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D051189:Murinae; D019590:Nucleocapsid Proteins / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics; D012376:Rodent Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary; D013045:Species Specificity
D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins