Organ tropism of murine coronavirus does not correlate with the expression levels of the membrane-anchored or secreted isoforms of the carcinoembryonic antigen-related cell adhesion molecule 1 receptor.
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is the sole known receptor of murine hepatitis virus (MHV) A59, but the available, often qualitative, data about CEACAM1 expression does not explain MHV organ tropism. Ceacam1 transcripts undergo alternative splicing resulting in multiple isoforms, including secreted CEACAM1 isoforms that can neutralize the virus. We determined the quantities of Ceacam1 transcripts encoding membrane-bound and secreted isoforms in mouse organs and a set of cell lines. In vivo, the lowest receptor mRNA levels were found in brain and muscle and these were similar to those in easily infectable cultured cells. While the quantities of the receptor transcripts varied between mouse organs, their abundance did not correlate with susceptibility to MHV infection. The proportion of transcripts encoding secreted isoforms also could not explain the selection of sites for virus replication, as it was constant in all organs. Our data suggest that neither of the two CEACAM1 isoforms defines MHV organ tropism.
Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands.
Olga
O
Slobodskaya
Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands.
Eric J
EJ
Snijder
Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands.
Willy J M
WJM
Spaan
D000818:Animals; D001921:Brain; D002272:Carcinoembryonic Antigen / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000235:genetics* / Q000378:metabolism; D018352:Coronavirus Infections / Q000235:genetics / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D005260:Female; D015870:Gene Expression; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D006517:Murine hepatitis virus / Q000235:genetics / Q000502:physiology*; D009132:Muscles / Q000378:metabolism* / Q000821:virology; D009928:Organ Specificity; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D011991:Receptors, Virus / Q000235:genetics* / Q000378:metabolism; D012376:Rodent Diseases / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D056189:Viral Tropism
D002272:Carcinoembryonic Antigen; C486183:Ceacam1 protein, mouse; D020033:Protein Isoforms; D011991:Receptors, Virus