Multiparametric assessment of Cd²+ cytotoxicity using MTT-based microfluidic image cytometry.
A modified MTT protocol-based microfluidic image cytometry (μFIC) was performed to assess Cd(2+) induced cytotoxicity. The expanded capabilities of μFIC, such as in situ measurement, high-throughput, and multiparametric analysis of adherent cells under precisely controlled chemical environments of microfluidic channels, were demonstrated in this study. Multiparametric analysis of μFIC data has enabled us to categorize the progress of cell death into at least four different subgroups based on their morphology and metabolic activity. These advantages of the MTT-based μFIC as a simpler, cheaper, and faster in vitro cell-based assay tool have many implications in biomedical, pharmaceutical, toxicological, and biological application areas, and we propose this technique as a future high throughput-high content screening (HT-HCS) platform for cytotoxicity assays and drug screening.
Department of Chemistry, Hanyang University, Seoul 133-791, Korea.
Kook Hee
KH
Lim
Jonghoon
J
Park
Seog Woo
SW
Rhee
Tae Hyun
TH
Yoon
D002104:Cadmium / Q000633:toxicity*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D004129:Dimethylpolysiloxanes; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D044085:Microfluidics / Q000379:methods*; D013228:Stearates / Q000378:metabolism*; D013997:Time Factors
D004129:Dimethylpolysiloxanes; D013228:Stearates; D002104:Cadmium; C070243:monooxyethylene trimethylolpropane tristearate