Osteoblast response to porous titanium surfaces coated with zinc-substituted hydroxyapatite.
BACKGROUND
The aims of this study were to deposit a zinc-hydroxyapatite (Zn-HA) coating on titanium surfaces by using the electrochemical process and investigate the cell response to the Zn-HA-coated titanium surface.


METHODS
Surface characteristics were evaluated by scanning electron microscopy (SEM) and inductively coupled plasma atomic emission spectroscopy (ICP-AES). Murine preosteoblast cell (MC3T3-E1) proliferation, alkaline phosphatase (ALP) activity, and osteocalcin release on Zn-HA-coated surfaces were compared with HA-coated surfaces.


RESULTS
Field-emission SEM observation showed rod-like HA crystals with a hexagonal cross-section on the HA-coated surface, although the hexagon of the cross-section of Zn-HA crystals became irregular. ICP-AES analysis showed that Zn was present in the Zn-HA coatings at a Zn/(Ca+Zn) molar ratio of 1.04%. Significant increases in cell proliferation, ALP activity on day 7, and osteocalcin production on day 14 (P < .05) were observed for Zn(2+)-containing HA-coated surfaces.


CONCLUSIONS
The present study showed that a Zn-HA coating deposited by using the electrochemical process enhances proliferation and differentiation of osteoblasts, which has the potential benefit to enhance implant osseointegration.
Department of Implantology, Stomatology Hospital, Medical School, Zhejiang University, Hangzhou, China.
Fan
F
Yang
Wen-jing
WJ
Dong
Fu-ming
FM
He
Xiao-xiang
XX
Wang
Shi-fang
SF
Zhao
Guo-li
GL
Yang
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry*; D004595:Electroplating; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D015675:Osteocalcin / Q000096:biosynthesis; D013054:Spectrophotometry, Atomic / Q000379:methods; D013499:Surface Properties; D014025:Titanium; D015032:Zinc / Q000494:pharmacology*
D020099:Coated Materials, Biocompatible; D015675:Osteocalcin; D017886:Durapatite; D014025:Titanium; D000469:Alkaline Phosphatase; D015032:Zinc