Short-chain fatty acid receptor GPR41-mediated activation of sympathetic neurons involves synapsin 2b phosphorylation.
Synapsins are neuronal phosphoproteins that coat synaptic vesicles and are believed to function in the regulation of neurotransmitter release. The signaling mechanism for short-chain free fatty acid (SCFA)-stimulated NE release was examined using primary-cultured mouse sympathetic cervical ganglion neurons. Pharmacological and knockdown experiments showed that activation of sympathetic neurons by SCFA propionate involves SCFA receptor GPR41 linking to G??-PLC?3-ERK1/2-synapsin 2 signaling. Further, synapsin 2b directly interacts with activated ERK1/2 and can be phosphorylated on serine when SCFA activates sympathetic neurons.
Department of Genomic Drug Discovery Science, Kyoto University Graduate School of Pharmaceutical Sciences, Sakyo-ku, Kyoto, Japan.
Daisuke
D
Inoue
Ikuo
I
Kimura
Masaki
M
Wakabayashi
Hiroki
H
Tsumoto
Kentaro
K
Ozawa
Takafumi
T
Hara
Yoshinori
Y
Takei
Akira
A
Hirasawa
Yasushi
Y
Ishihama
Gozoh
G
Tsujimoto
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000502:physiology*; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000502:physiology*; D013564:Sympathetic Nervous System / Q000166:cytology; D016704:Synapsins / Q000378:metabolism*
D017931:DNA Primers; C506482:Gpcr41 protein, mouse; D034741:RNA, Small Interfering; D043562:Receptors, G-Protein-Coupled; D016704:Synapsins; D048049:Extracellular Signal-Regulated MAP Kinases