Histone deacetylase-4 is required during early cranial neural crest development for generation of the zebrafish palatal skeleton.
BACKGROUND
Histone deacetylase-4 (Hdac4) is a class II histone deacetylase that inhibits the activity of transcription factors. In humans, HDAC4 deficiency is associated with non-syndromic oral clefts and brachydactyly mental retardation syndrome (BDMR) with craniofacial abnormalities.


RESULTS
We identify hdac4 in zebrafish and characterize its function in craniofacial morphogenesis. The gene is present as a single copy, and the deduced Hdac4 protein sequence shares all known functional domains with human HDAC4. The zebrafish hdac4 transcript is widely present in migratory cranial neural crest (CNC) cells of the embryo, including populations migrating around the eye, which previously have been shown to contribute to the formation of the palatal skeleton of the early larva. Embryos injected with hdac4 morpholinos (MO) have reduced or absent CNC populations that normally migrate medial to the eye. CNC-derived palatal precursor cells do not recover at the post-migratory stage, and subsequently we found that defects in the developing cartilaginous palatal skeleton correlate with reduction or absence of early CNC cells. Palatal skeletal defects prominently include a shortened, clefted, or missing ethmoid plate, and are associated with a shortening of the face of young larvae.


CONCLUSIONS
Our results demonstrate that Hdac4 is a regulator of CNC-derived palatal skeletal precursors during early embryogenesis. Cleft palate resulting from HDAC4 mutations in human patients may result from defects in a homologous CNC progenitor cell population.
Institute of Neuroscience, 1254 University of Oregon, Eugene, OR 97403, USA. april@uoneuro.uoregon.edu
April
A
DeLaurier
Yukio
Y
Nakamura
Ingo
I
Braasch
Vishesh
V
Khanna
Hiroyuki
H
Kato
Shigeyuki
S
Wakitani
John H
JH
Postlethwait
Charles B
CB
Kimmel
D000818:Animals; D002356:Cartilage / Q000002:abnormalities / Q000196:embryology; D006257:Head / Q000196:embryology; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D060172:Morpholinos / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology* / Q000378:metabolism; D010159:Palate / Q000002:abnormalities* / Q000196:embryology*; D012097:Repressor Proteins / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D060172:Morpholinos; D012097:Repressor Proteins; D029961:Zebrafish Proteins; C400300:HDAC4 protein, human; C576359:HDAC4 protein, zebrafish; D006655:Histone Deacetylases