Green synthesis, characterization and anticancer potential of platinum nanoparticles Bioplatin.
OBJECTIVE
In the present study, the anticancer potential of platinum nanoparticles Bioplatin is explored and the mode of interactions of Bioplatin with calf thymus DNA and honey was analyzed.


METHODS
Bioplatin was synthesized with the help of green nanotechnology and characterized by particle size, zeta potential and surface morphology. The interaction of Bioplatin with DNA and honey was also checked with the help of circular dichroism spectroscopy and Fourier-transform infrared spectroscopy, respectively. The anticancer potential of Bioplatin was evaluated on peripheral blood mononuclear cells and A375 cells in vitro by analyzing results of MTT (3-(4,5)-dimethyl-thiahiazo-(-z-y1)-3,5-di-phenytetrazoliumromide), fluorescence microscopic studies and DNA fragmentation assay.


RESULTS
Bioplatin exhibited a small particle size of 137.5 nm and a surface charge of -35.8 mV. Bioplatin interacted with DNA and brought in effective changes in structure and conformation of DNA, and formed a new complex that increased its stability of DNA intercalated with the base pair of DNA. In vitro studies demonstrated that Bioplatin arrested cell proliferation, and induced chromatin condensation and internucleosomal DNA fragmentation.


CONCLUSIONS
Bioplatin induces apoptosis in cancer cells and may have some beneficial effect against human carcinoma. It interacts with DNA, brings stabilization to DNA, and thus prevents the replication of DNA.
Research and Development Section, Cell and Molecular Biology Department, Rasayani Biologics Private Limited, Pune 411030, India. ssbku13@gmail.com
Yogesh
Y
Bendale
Vineeta
V
Bendale
Saili
S
Paul
Soumya Sundar
SS
Bhattacharyya
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000138:chemical synthesis / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002417:Cattle; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D004247:DNA; D053938:DNA Fragmentation; D055772:Green Chemistry Technology; D006801:Humans; D053758:Nanoparticles; D036103:Nanotechnology; D010316:Particle Size; D010984:Platinum / Q000008:administration & dosage / Q000494:pharmacology
D000970:Antineoplastic Agents; D010984:Platinum; D004247:DNA; C427203:calf thymus DNA