Application of pheB as a reporter gene for Geobacillus spp., enabling qualitative colony screening and quantitative analysis of promoter strength.
The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3-dioxygenase (C23O), catalyzes the formation of 2-hydroxymuconic semialdehyde, which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.
Department of Life Sciences, Imperial College London, London, United Kingdom.
Jeremy
J
Bartosiak-Jentys
Kirstin
K
Eley
David J
DJ
Leak
D050557:Catechol 2,3-Dioxygenase / Q000378:metabolism; D005285:Fermentation; D015870:Gene Expression; D017930:Genes, Reporter; D005827:Genetics, Microbial / Q000379:methods*; D056506:Geobacillus / Q000235:genetics*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008403:Mass Screening; D011401:Promoter Regions, Genetic
D007770:L-Lactate Dehydrogenase; D050557:Catechol 2,3-Dioxygenase