Thermoresponsive micropatterned substrates for single cell studies.
We describe the design of micropatterned surfaces for single cell studies, based on thermoresponsive polymer brushes. We show that brushes made of poly(N-isopropylacrylamide) grafted at high surface density display excellent protein and cell anti-adhesive properties. Such brushes are readily patterned at the micron scale via deep UV photolithography. A proper choice of the adhesive pattern shapes, combined with the temperature-dependent swelling properties of PNIPAM, allow us to use the polymer brush as a microactuator which induces cell detachment when the temperature is reduced below [Formula: see text]C.
Laboratoire Interdisciplinaire de Physique UMR 5588, Univ. Grenoble 1/CNRS, Grenoble, France.
Kalpana
K
Mandal
Martial
M
Balland
Lionel
L
Bureau
D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D008853:Microscopy; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature
D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; C052970:poly-N-isopropylacrylamide