The expression of a naturally occurring, truncated allele of an α-SNAP gene suppresses plant parasitic nematode infection.
Transcriptional mapping experiments of the major soybean cyst nematode resistance locus, rhg1, identified expression of the vesicular transport machinery component, α soluble NSF attachment protein (α-SNAP), occurring during defense. Sequencing the α-SNAP coding regions from the resistant genotypes G. max                         ([Peking/PI 548402]) and G. max                         ([PI 437654]) revealed they are identical, but differ from the susceptible G. max                         ([Williams 82/PI 518671]) by the presence of several single nucleotide polymorphisms. Using G. max                         ([Williams 82/PI 518671]) as a reference, a G → T(2,822) transversion in the genomic DNA sequence at a functional splice site of the α-SNAP([Peking/PI 548402]) allele produced an additional 17 nucleotides of mRNA sequence that contains an in-frame stop codon caused by a downstream G → A(2,832) transition. The G. max                         ([Peking/PI 548402]) genotype has cell wall appositions (CWAs), structures identified as forming as part of a defense response by the activity of the vesicular transport machinery. In contrast, the 17 nt α-SNAP([Peking/PI 548402]) mRNA motif is not found in G. max                         ([PI 88788]) that exhibits defense to H. glycines, but lack CWAs. The α-SNAP([PI 88788]) promoter contains sequence elements that are nearly identical to the α-SNAP([Peking/PI 548402]) allele, but differs from the G. max                         ([Williams 82/PI 518671]) ortholog. Overexpressing the α-SNAP([Peking/PI 548402]) allele in the susceptible G. max                         ([Williams 82/PI 518671]) genotype suppressed H. glycines infection. The experiments indicate a role for the vesicular transport machinery during infection of soybean by the soybean cyst nematode. However, increased GmEREBP1, PR1, PR2, PR5 gene activity but suppressed PR3 expression accompanied the overexpression of the α-SNAP([Peking/PI 548402]) allele prior to infection.
Department of Biological Sciences, Mississippi State University, Mississippi State, MS 39762, USA.
Prachi D
PD
Matsye
Gary W
GW
Lawrence
Reham M
RM
Youssef
Kyung-Hwan
KH
Kim
Katheryn S
KS
Lawrence
Benjamin F
BF
Matthews
Vincent P
VP
Klink
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D060467:Disease Resistance / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D006790:Host-Parasite Interactions; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins / Q000235:genetics* / Q000378:metabolism; D013025:Glycine max / Q000145:classification / Q000235:genetics* / Q000469:parasitology; D013045:Species Specificity; D014431:Tylenchoidea / Q000502:physiology
D010940:Plant Proteins; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins