Visualization and analysis of vascular receptors using confocal laser scanning microscopy and fluorescent ligands.
The use of fluorescent ligands to analyze receptor distribution is increasing in popularity. This is due to the ever growing number of fluorescent ligands and the increased sensitivity of microscope-based technologies. Image-analysis methods have advanced to a stage where quantification of fluorescent signals is relatively simple (if used appropriately). In this chapter we describe a method of analyzing the 2D and 3D distribution of fluorescent ligands in segments of blood vessels. In addition, we introduce the issues surrounding the accurate analysis of colocalization of two different fluorescent ligands.
College of Medical, Veterinary & Life Sciences, School of Life Sciences, University of Glasgow, Glasgow, UK. Craig.Daly@Glasgow.ac.uk
Craig J
CJ
Daly
Ingela
I
Parmryd
John C
JC
McGrath
D000818:Animals; D001808:Blood Vessels / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional; D008024:Ligands; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D011485:Protein Binding; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism*
D005456:Fluorescent Dyes; D008024:Ligands; D011506:Proteins