The effects of hydrogen sulfide under sevoflurane administration against ischemia and reperfusion injury in isolated rat heart.
BACKGROUND
Hydrogen sulfide (H(2)S) produces a protective effect against myocardial ischemia and reperfusion injury. Sevoflurane, which is used for anesthesia in cardiac problem patients, also has a protective effect. This study is designed to reveal the effects of H(2)S under sevoflurane using rat hearts.


METHODS
The hearts were Langendorff-perfused, subjected to 30 minutes ischemia and 60 minutes reperfusion. Group I was a control group. The other groups were pretreated for 15 minutes before ischemia as follows: 1.6% sevoflurane for group S; 18.5 µM H(2)S S for group H; and 1.6% sevoflurane and 18.5 µM H(2)S simultaneously for group HS. Hemodynamics and the infarct size were measured.


RESULTS
Group HS presented depressed hemodynamics during pretreatment. LV function in group HS achieved better recovery than group I after reperfusion. The infarct size of groups S, H and HS was smaller than group I, while there were no differences between groups S, H and HS.


CONCLUSIONS
Exogenous H(2)S did not enhance the preconditioning effects of sevoflurane. Rather, the results suggest that H(2)S under sevoflurane might depress hemodynamics.
Department of Anesthesiology and Pain Medicine, Korea University Guro Hospital, Seoul, Korea.
Dong Kyu
DK
Lee
Sang Ho
SH
Lim
Nan Suk
NS
Kim