Attenuating effects of granulocyte-colony stimulating factor (G-CSF) in radiation induced intestinal injury in mice.
Gastrointestinal injury is a major cause of death following exposure to high levels of radiation, and no effective treatments are currently available. In this study, we examined the capacity of granulocyte colony-stimulating factor (G-CSF) to mitigate intestinal injury in, and improve survival of, C3H/HeN mice given a lethal dose (12 Gy) of radiation to the abdomen. G-CSF (100 μg/kg body weight) was injected subcutaneously daily for 3 days after irradiation and shown to improve survival and intestinal morphology at 3.5 days compared with saline-injected controls. The morphological features improved by G-CSF included crypt number and depth, villous length, and the length of basal lamina of 10 enterocytes. G-CSF also normalized the levels of circulating tumor necrosis factor alpha and attenuated the loss of peripheral neutrophils, caused by radiation-induced myelosuppression. In conclusion, our results suggest that G-CSF enhanced the survival of irradiated mice and minimized the effects of radiation on gastrointestinal injury.
Research Center, Dongnam Institute of Radiological & Medical Sciences (DIRAMS), Busan 619-953, Republic of Korea. kmyang@dirams.re.kr
Joong-Sun
JS
Kim
Seung-Bum
SB
Ryoo
Kyu
K
Heo
Joong-Gook
JG
Kim
Tae-Gen
TG
Son
Changjong
C
Moon
Kwangmo
K
Yang
D000818:Animals; D016207:Cytokines / Q000097:blood; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D007583:Jejunum / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D015996:Survival Rate
D016207:Cytokines; D016179:Granulocyte Colony-Stimulating Factor