A new fluorescent and colorimetric probe for Cu2+ in live cells.
Based on a change in structure between spirocyclic (non-fluorescent) and ring-open (fluorescent) forms of rhodamine-based dyes, a new fluorescent and colorimetric Cu(2+) probe was designed and synthesized. Upon treatment with Cu(2+), the weakly fluorescent probe exhibited a strong fluorescence response with high selectivity. In addition, the turn-on fluorescent probe upon the addition of Cu(2+) was applied in live cell imaging.
Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
Wei-Yong
WY
Liu
Hai-Ying
HY
Li
Bao-Xiang
BX
Zhao
Jun-Ying
JY
Miao
D002470:Cell Survival; D002478:Cells, Cultured; D003124:Colorimetry; D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D005456:Fluorescent Dyes; D012235:Rhodamines; D003300:Copper