Preclinical pharmacokinetic analysis of armillarisin succinate ester in mouse plasma and tissues by LC-MS/MS.
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated to determine the concentration of armillarisin succinate ester in mouse plasma and tissues, used for preclinical evaluation. Bavachin was employed as the internal standard. Separation was performed on a 3.5 µm Zorbax SB-C(18) column (30 × 2.1 mm), with a mobile phase consisting of methanol and aqueous 20 mm ammonium acetate. Both analyte and internal standard were determined using electrospray ionization and the MS data acquisition was via selected ion monitoring in negative scanning mode. Quantification was performed using the transitions m/z 333 → 233 and 323 → 221 for armillarisin succinate ester and internal standard, respectively. The method was validated with respect to linearity, accuracy, precision, recovery and stability. This assay has been successfully applied to a pharmacokinetic and tissue distribution study after intravenous injection of ASE in mouse in a dose of 10 mg/kg.
Institute of Pharmaceutical Analysis and Drug Screening, Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei Shiyan, China. cqh77@163.com
Qinhua
Q
Chen
Peng
P
Li
Jinfeng
J
Zhang
Jun
J
Zhu
D000818:Animals; D001578:Benzopyrans / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004952:Esters / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D005260:Female; D016014:Linear Models; D008297:Male; D051379:Mice; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019802:Succinic Acid / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution
D001578:Benzopyrans; D004952:Esters; D019802:Succinic Acid; C023435:armillarisin A