[Generation of goat H-FABP overexpression transgenic mice by testicular injection].
To explore the possibility of transgenic animals by testicular injection, the goat heart-type fatty acid binding protein (H-FABP) expression vector pEGFP-H-FABP was injected into the testis of 6 mice randomly by liposome mediated transfection. By detection of testis slice, sperm fluorescence and sperm DNA PCR, the exogenous gene was expressed in the parental mice. The exogenous gene was expressed at different levels in both the F1 generation mice gave birthed by treated male mice and normal female mice and the F2 generation mice generated by mating F1 could be detected that the exogenous gene expressed at different levels with the positive rates of 4% and 30.23%, respectively. The results suggested that testicu-lar injection, as an effective method to generate transgenic animal, could realize the stable integration of exogenous gene. The amelioration and maturity of testicular injection provides theoretical and practical significance in generation of trans-genic animals and even in the animal trait improvement and breeding.
Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. yinjianbin0312@gmail.com
Yan-Hui
YH
Yin
Min
M
Sun
Ting-Feng
TF
Chen
Ya-Ni
YN
Zhang
Cai-Ye
CY
Zhu
Wei
W
Li
Bi-Chun
BC
Li
D000818:Animals; D050556:Fatty Acid-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018014:Gene Transfer Techniques; D006041:Goats; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
D050556:Fatty Acid-Binding Proteins