Functional monolithic materials for boronate-affinity chromatography via Schrock catalyst-triggered ring-opening metathesis polymerization.
Monolithic polymeric materials are prepared via ring-opening metathesis copolymerization of norborn-2-ene with 1,4,4a,5,8,8a-hexahydro-1,4,5,8-exo,endo-dimethanonaphthalene in the presence of macro- and microporogens, that is, of n-hexane and 1,2-dichloroethane, using the Schrock catalyst Mo(N-2,6-(2-Pr)(2) -C(6) H(3) )(CHCMe(2) Ph)(OCMe(3) )(2) . Functionalization of the monolithic materials is accomplished by either terminating the living metal alkylidenes with various functional aldehydes or by post-synthesis grafting with norborn-5-en-2-ylmethyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzoate. Finally, boronate-grafted monolithic columns (100 × 3 mm i.d.) are successfully applied to the affinity chromatographic separation of cis-diol-based biomolecules.
Institute of Polymer Chemistry, University of Stuttgart, Stuttgart, Germany. ipobuch@ipoc.uni-stuttgart.de
Rajendar
R
Bandari
Michael R
MR
Buchmeiser
D000327:Adsorption; D000447:Aldehydes / Q000737:chemistry; D002384:Catalysis; D002846:Chromatography, Affinity / Q000295:instrumentation*; D015394:Molecular Structure; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
D000447:Aldehydes; D011108:Polymers