SET oncogene is upregulated in pediatric acute lymphoblastic leukemia.
OBJECTIVE
The SET gene is a target of chromosomal translocations in acute leukemia and encodes a widely expressed multifunctional phosphoprotein. It has been shown that SET is upregulated in BCR-ABL1-positive cell lines, patient-derived chronic myeloid leukemia CD34-positive cells, and some solid tumors.


METHODS
We determined the expression level of SET in 59 pediatric acute lymphoblastic leukemia patients who were BCR-ABL-negative using quantitative real-time reverse-transcriptase-polymerase chain reaction. Results. We showed that SET expression was significantly upregulated in 96.5% of B-acute lymphoblastic leukemia (28 of 29; 16.6 fold) and 93% of T-acute lymphoblastic leukemia (28 of 30; 47.6 fold) patients. This upregulation was not associated with any clinical features or overall and relapse-free survival.


CONCLUSIONS
Our results showed that SET is significantly overexpressed in pediatric acute lymphoblastic leukemia samples, and an increased level of SET might contribute to leukemic process.
Istanbul University, Institute of Experimental Medicine, Department of Genetics, Istanbul, Turkey.
Sema
S
Sirma Ekmekci
Cumhur
C
G Ekmekci
Ayten
A
Kandilci
Cagri
C
Gulec
Meral
M
Akbiyik
Zeliha
Z
Emrence
Neslihan
N
Abaci
Zeynep
Z
Karakas
Leyla
L
Agaoglu
Aysegul
A
Unuvar
Sema
S
Anak
Omer
O
Devecioglu
Duran
D
Ustek
Gerard
G
Grosveld
Ugur
U
Ozbek
D000293:Adolescent; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004268:DNA-Binding Proteins; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056488:Histone Chaperones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D009363:Neoplasm Proteins; C513473:SET protein, human; D014157:Transcription Factors