Success rate of collagen gel droplet-embedded culture drug sensitivity test in colorectal cancer: are antibiotics a prerequisite for specimen irrigation?
The collagen gel droplet-embedded culture drug sensitivity test (CD-DST) is one of the best chemosensitivity tests owing to its high success rate. However, CD-DST is often a culture method, and contamination is a serious problem, especially in the case of colorectal cancer, which is contaminated by enteric bacteria. It has been reported that the success rate of CD-DST is 64.0% in the case of colorectal cancer. Therefore, the sampling and washing of specimens before culture are extremely important. By washing specimens carefully with normal saline containing antibiotics, we achieved a success rate of 85.3% in the case of colorectal cancer. To improve the success rate, we started specimen irrigation with a large amount of normal saline in January 2007. As a result, a success rate exceeding 90% was acquired. For the success of CD-DST for colorectal cancer, it is important to irrigate specimens many times with a large amount of normal saline.
Surgery for Organ Function and Biological Regulation, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan. y-tak@nms.ac.jp
Takeshi
T
Yamada
Hayato
H
Kan
Satoshi
S
Matsumoto
Yoshikazu
Y
Kanazawa
Michihiro
M
Koizumi
Junpei
J
Sasaki
Aya
A
Tani
Kimiyoshi
K
Yokoi
Eiji
E
Uchida
D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry*; D015179:Colorectal Neoplasms / Q000382:microbiology*; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005260:Female; D005782:Gels / Q000737:chemistry*; D006801:Humans; D008297:Male; D013048:Specimen Handling / Q000379:methods*; D046509:Tissue Culture Techniques / Q000379:methods*
D000900:Anti-Bacterial Agents; D005782:Gels; D003094:Collagen