Comparative antigenic proteins and proteomics of pathogenic Yersinia enterocolitica bio-serotypes 1B/O: 8 and 2/O: 9 cultured at 25°C and 37°C.
Yersinia enterocolitica is a Gram-negative enteric pathogen responsible for a number of gastrointestinal disorders; the most pathogenic bio-serotype is 1B/O: 8. In this study, we compared the antigenicity of the outer membrane proteins and proteomics of the whole-cell proteins of a pathogenic bio-serotype 2/O: 9 isolated in China and a bio-serotype 1B/O: 8 strain isolated in Japan. Using two-dimensional gel electrophoresis, we showed that the outer membrane proteins A (OmpA), C (OmpC) and F (OmpF) were the major antigens for both strains, although proteins located on the bacterial cell membrane and enzymes involved in energy metabolism were also identified as antigenic. We compared the whole-cell proteins of the two strains cultured at 25°C and 37°C and found portions of the outer membrane proteins (OmpX, OmpF and OmpA) were downregulated when the bacteria were cultured at 37°C, whereas urease subunit gamma (UreA), urease subunit alpha (UreC) and urease accessory protein (UreE), which are involved in urease synthesis, were upregulated when the bacteria were grown at 37°C. These observations will lay a foundation to selection of diagnostic markers for pathogenic Yersinia enterocolitica, and maybe contribute to choose the vaccine targets.
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
Wenpeng
W
Gu
Xin
X
Wang
Haiyan
H
Qiu
Xia
X
Luo
Di
D
Xiao
Yuchun
Y
Xiao
Liuying
L
Tang
Biao
B
Kan
Huaiqi
H
Jing
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D001431:Bacteriological Techniques / Q000379:methods; D002462:Cell Membrane / Q000737:chemistry / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D005260:Female; D018272:Porins / Q000032:analysis / Q000276:immunology; D020543:Proteome / Q000032:analysis* / Q000276:immunology; D040901:Proteomics / Q000379:methods*; D011817:Rabbits; D013696:Temperature; D015008:Yersinia enterocolitica / Q000254:growth & development / Q000276:immunology*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C113724:OmpC protein; C109237:OmpF protein; D018272:Porins; D020543:Proteome; C474041:ureC protein, Yersinia enterocolitica; C078167:OMPA outer membrane proteins