CTLA-4 exon 1 +49A/G polymorphism is associated with renal involvement in pediatric Henoch-Schönlein purpura.
BACKGROUND
Henoch-Schönlein purpura (HSP) is a multisystemic vasculitis of unknown etiology. Cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) and CD28 have been reported to be important candidate genes for conferring susceptibility to autoimmunity. In this study, we investigated the correlation of CTLA-4 and CD28 gene polymorphisms with HSP in children with and without renal involvement.


METHODS
The CTLA-4 exon 1 +49A/G, promoter -318C/T and CD28 IVS3 +17T/C single nucleotide polymorphisms (SNPs) were genotyped in 110 children with HSP and 90 ethnically matched healthy controls through restriction fragment-length polymorphism (RFLP).


RESULTS
The CTLA-4 (+49) GG genotype and G allele (GG + AG genotype) were more common in HSP patients with renal involvement (n = 52) than in HSP patients without renal involvement (n = 58) (P = 0.019 and 0.001, respectively). There were no significant differences in the prevalence of CTLA-4 (+49 A/G), (-318C/T) and CD28 IVS3 (+17 /T/C) polymorphisms between HSP patients and controls.


CONCLUSIONS
Our findings suggest that the CTLA-4 +49 GG genotype and G allele may contribute to increased risk for the development of renal damage in HSP patients.
Department of Pediatrics, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, 637000, People's Republic of China.
Jian-Jun
JJ
Wang
Department of Traditional Chinese Medicine, Xi'an Children's Hospital, Xi'an University of Medicine, Xi'an, Shanxi, 710033, People's Republic of China.
Yan-Ping
YP
Shi
Department of Pediatrics, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, 637000, People's Republic of China.
Huang
H
Yue
Department of Pediatrics, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, 637000, People's Republic of China.
Wu
W
Chun
Department of Pediatrics, Chinese PLA General Hospital, Beijing, 100853, People's Republic of China. zouliping21@hotmail.com.
Li-Ping
LP
Zou
D000293:Adolescent; D000367:Age Factors; D054458:Amplified Fragment Length Polymorphism Analysis; D018106:CD28 Antigens / Q000235:genetics; D060908:CTLA-4 Antigen / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005091:Exons; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006417:Hematuria / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D006801:Humans; D011695:IgA Vasculitis / Q000150:complications / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D007668:Kidney / Q000276:immunology* / Q000473:pathology; D008297:Male; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012307:Risk Factors
D018106:CD28 Antigens; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human