Resveratrol prevents nicotine-induced teratogenesis in cultured mouse embryos.
Nicotine, a major toxic component in tobacco smoke, leads to severe embryonic damage during organogenesis in embryos. We investigated whether resveratrol would positively influence nicotine-induced teratogenesis in mouse embryos (embryonic day 8.5) cultured for 48 h using a whole embryo culture system. Embryos exposed to nicotine (1mM) revealed significantly severe morphological anomalies, increased levels of caspase-3 mRNA and lipid peroxidation, and decreased levels of cytoplasmic superoxide dismutase (SOD), mitochondrial manganese SOD, cytosolic glutathione peroxidase, phospholipid hydroperoxide glutathione peroxidase, hypoxia-inducible factor 1α, Bcl-x(L), and sirtuin1 (SIRT1) mRNAs and SOD activity compared to those in the normal control group. However, when resveratrol (1×10(-8) μM or 1×10(-7) μM) was added concurrently to the embryos exposed to nicotine, all the parameters in above improved conspicuously. These findings indicate that resveratrol has a noted protective effect against nicotine-induced teratogenesis in mouse embryos through its antioxidative and anti-apoptotic effects.
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, South Korea.
Chunmei
C
Lin
Jung-Min
JM
Yon
A Young
AY
Jung
Jong Geol
JG
Lee
Ki Youn
KY
Jung
Jong-Koo
JK
Kang
Beom Jun
BJ
Lee
Young Won
YW
Yun
Sang-Yoon
SY
Nam
D000014:Abnormalities, Drug-Induced / Q000517:prevention & control*; D000818:Animals; D053148:Caspase 3 / Q000235:genetics; D004622:Embryo, Mammalian / Q000187:drug effects*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000235:genetics; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009538:Nicotine / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D020011:Protective Agents / Q000494:pharmacology*; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000235:genetics; D013267:Stilbenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013723:Teratogens / Q000633:toxicity*; D051020:bcl-X Protein / Q000235:genetics; D000094765:Glutathione Peroxidase GPX1
C494812:Bcl2l1 protein, mouse; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D020011:Protective Agents; D013267:Stilbenes; D013723:Teratogens; D051020:bcl-X Protein; D009538:Nicotine; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D053148:Caspase 3; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D000077185:Resveratrol; D000094765:Glutathione Peroxidase GPX1; C000722068:Gpx1 protein, mouse