Effect of local environment, fibrin, and basic fibroblast growth factor incorporation on a canine autologous model of bioengineered cartilage tissue.
We developed a technique to form a bioabsorbable synthetic polymer (polyglycolic acid, PGA) combined with a natural polymer (fibrin) to serve as a scaffold to help retain seeded cells and improve the seeding efficiency of chondrocytes in an implantable construct. This approach was evaluated in a canine autologous implant model of bioengineered cartilage. The implantation site (subcutaneous or intrafascial) and the use of basic fibroblast growth factor (b-FGF) were also evaluated with this system. The intrafascial implantation site yielded optimal results, especially when used in conjunction with fibrin and a b-FGF sustained-release system incorporated into the complex. A thicker, more sustained cartilagenous layer was formed, with a more vascularized outer fibrous supporting tissue layer. This combined approach of implant environment selection, natural polymer for cell retention, and growth factor supplementation offers a more optimized method for generating bioengineered auricular cartilage.
Department of Plastic and Reconstructive Surgery, Kinki University School of Medicine, Osaka, Japan.
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T
Morotomi
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Wada
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Uehara
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M
Enjo
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Isogai
D000818:Animals; D001698:Biomedical Engineering / Q000379:methods*; D002356:Cartilage / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D004285:Dogs; D005260:Female; D005337:Fibrin / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D023822:Tissue Engineering / Q000379:methods*
D016222:Fibroblast Growth Factor 2; D005337:Fibrin