Histone deacetylase 3 mediates allergic skin inflammation by regulating expression of MCP1 protein.
We have shown the induction of histone deacetylase 3 (HDAC3) in antigen-stimulated rat basophilic leukemia cells via NF-κB. We investigated the role of HDAC3 in allergic skin inflammation. We used a BALB/c mouse model of triphasic cutaneous anaphylaxis (triphasic cutaneous reaction; TpCR) and passive cutaneous anaphylaxis (PCA) to examine the role of HDAC3 in allergic skin inflammation. Triphasic cutaneous reaction involved induction of HDAC3 and was mediated by HDAC3. HDAC3 showed an interaction with FcεRIβ. Trichostatin A (TSA), an inhibitor of HDAC(s), disrupted this interaction. Cytokine array analysis showed that the down-regulation of HDAC3 led to the decreased secretion of monocyte chemoattractant protein 1 (MCP1). FcεRI was necessary for induction of HDAC3 and MCP1. ChIP assays showed that HDAC3, in association with Sp1 and c-Jun, was responsible for induction of MCP1 expression. TSA exerted a negative effect on induction of MCP1. HDAC3 exerted a negative regulation on expression of HDAC2 via interaction with Rac1. The down-regulation of HDAC3 or inactivation of Rac1 induced binding of HDAC2 to MCP1 promoter sequences. TSA exerted a negative effect on HDAC3-mediated TpCR. The BALB/c mouse model of PCA involved induction of HDAC3 and MCP1. HDAC3 and MCP1 were necessary for PCA that involved ear swelling, enhanced vascular permeability, and angiogenesis. Recombinant MCP1 enhanced β-hexosaminidase activity and histamine release and also showed angiogenic potential. TSA exerted a negative effect on PCA. Our data show HDAC3 as a valuable target for the development of allergic skin inflammation therapeutics.
Department of Biochemistry, Kangwon National University, Chunchon 200-701, Korea.
Youngmi
Y
Kim
Kyungjong
K
Kim
Deokbum
D
Park
Eunmi
E
Lee
Hansoo
H
Lee
Yun-Sil
YS
Lee
Jongseon
J
Choe
Dooil
D
Jeoung
D000818:Animals; D002199:Capillary Permeability / Q000276:immunology; D002460:Cell Line; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D017449:Dermatitis, Allergic Contact / Q000201:enzymology* / Q000276:immunology; D004139:Dinitrofluorobenzene / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D056464:Histone Deacetylase 2 / Q000235:genetics; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006655:Histone Deacetylases / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic; D009479:Neuropeptides / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D017455:Receptors, IgE / Q000378:metabolism; D015533:Transcriptional Activation; D020744:rac GTP-Binding Proteins / Q000378:metabolism; D020830:rac1 GTP-Binding Protein; D000099186:Histone Deacetylase 3
D018932:Chemokine CCL2; D004139:Dinitrofluorobenzene; D056464:Histone Deacetylase 2; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases; D006877:Hydroxamic Acids; D009479:Neuropeptides; D017455:Receptors, IgE; D020744:rac GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein; D000099186:Histone Deacetylase 3; C491768:Ccl2 protein, mouse; C484602:Rac1 protein, mouse; C012589:trichostatin A; C533424:Hdac2 protein, mouse