[Influence of jinlingzi powder with different compatibility on activity of cytochrome P1 A2 from rat liver microsomes].
To illustrate the compability rule of Jinlingizi powder, by investigating the effects of Jinlingzi Powder with different compatibility on the enzymatic activity of cytochrome P1 A2 (CYP1A2) from rat liver microsome. The different compability of Jinlingizi powder is designed, based on the orthogonal array L9 (3(4)). In vitro test, rat liver microsomes incubation system is applied to detect the 50% inhibitory concentraton of Jinlingzi powder with different compatibility to cytochrome P1A2 (CYP1A2) enzyme. In vivo experiments, rats is treated orally with the different compability of Jinlingizi powder for 5 days, then be injected with probe drug phenacetin. The biosample from liver tissue is obtained by microdialysis probe, then analysisd by HPLC. The concentration-time data are modulated by software WinNonlin. IC50 data show no significant inhibitory activty to cytochrome P1 A2. Acetaminophen and phenacetin PK parameters indicate that the different compability of Jinlingizi powder can modulate the CYP 1A2 mediated metabolism, which is associate with the compatibility of Jinlingzi powder.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Long
L
Cheng
Lan
L
Wang
Yanli
Y
Wang
Rixin
R
Liang
Weipeng
W
Yang
Wei
W
Wang
Nan
N
Hu
Xiaojie
X
Yin
Xiaogang
X
Weng
Yiwei
Y
Wang
Qing
Q
Yang
D000818:Animals; D065609:Cytochrome P-450 CYP1A2 Inhibitors; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D008516:Medicine, Chinese Traditional; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D011208:Powders; D051381:Rats; D017208:Rats, Wistar
D065609:Cytochrome P-450 CYP1A2 Inhibitors; D004365:Drugs, Chinese Herbal; D011208:Powders