Removal of organic toxic chemicals using the spent mushroom compost of Ganoderma lucidum.
The removal of the organic toxic chemicals di-n-butyl phthalate (DBP), di-2-ethyl hexyl phthalate (DEHP), nonylphenol (NP), and bisphenol-A (BPA) by laccase obtained from the spent mushroom compost (SMC) of the white rot fungi, Ganoderma lucidum, was investigated. The optimal conditions for the extraction of laccase from SMC required using sodium acetate buffer (pH 5.0, solid : solution ratio 1 : 5), and extraction over 3 h at 4 °C. The removal of NP was enhanced by adding CuSO(4) (1 mM), MnSO(4) (0.5 mM), tartaric acid (20 mM), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS; 1 mM), and 1-hydroxybenzotriazole (HBT; 20 mg L(-1)), with ABTS yielding a higher NP removal efficiency than the other additives. At a concentration of 2 mg L(-1), DBP, DEHP, NP, and BPA were almost entirely removed by laccase after incubation for 1 day. The removal efficiencies, in descending order of magnitude, were DBP > BPA > NP > DEHP. We believe that these findings could provide useful information for improving the efficiency of the removal of organic toxic chemicals in the environment.
Department of Civil and Ecological Engineering, I Shou University, Kaohsiung 84001, Taiwan.
Chien-Sen
CS
Liao
Shaw-Ying
SY
Yuan
Bau-Huei
BH
Hung
Bea-Ven
BV
Chang
D001673:Biodegradation, Environmental; D015386:Hazardous Substances / Q000032:analysis / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism; D010636:Phenols / Q000032:analysis / Q000378:metabolism; D010795:Phthalic Acids / Q000032:analysis / Q000378:metabolism; D020940:Reishi / Q000254:growth & development / Q000378:metabolism*; D012987:Soil; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
D015386:Hazardous Substances; D010636:Phenols; D010795:Phthalic Acids; D012987:Soil; D012989:Soil Pollutants; D042845:Laccase