[Comparison of allelic frequencies of 15 short tandem repeat loci between chronic myeloid leukemia patients and non-related healthy individuals].
OBJECTIVE
To compare allelic frequencies of 15 short tandem repeat (STR) loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA) between chronic myeloid leukemia (CML) patients and non-related healthy individuals from Changzhou region in order to predict genes related with the CML.


METHODS
Blood samples were collected from 745 healthy subjects and 132 CML patients with complete remission. Genotypes were determined with gene scan technology and multiplex PCR with fluorescence-labeled primers. Allelic polymorphisms of 15 STR loci were compared between the two groups. Potential genes related with CML were predicted with statistical analysis of differences in allelic frequencies.


RESULTS
Allelic frequencies of 3 loci, including CSF1PO, vWA and TPOX, showed a significant difference (P<0.05) between the two groups.


CONCLUSIONS
CSF1PO, vWA and TPOX loci may be related with CML, albeit that the exact biologic mechanisms is unclear.
Laboratory of Hematology, the Third Affiliated Hospital of Soochow University, the First People's Hospital of Changzhou, Changzhou, Jiangsu 213003, P. R. China. wzl1100@sina.com
Zhi-lin
ZL
Wang
Li
L
Dai
Sang
S
Li
Guo-qiang
GQ
Qiu
Hao-qing
HQ
Wu
D005787:Gene Frequency; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic