Neural stem cell specific fluorescent chemical probe binding to FABP7.
Fluorescent small molecules have become indispensable tools for biomedical research along with the rapidly developing optical imaging technology. We report here a neural stem cell specific boron-dipyrromethane (BODIPY) derivative compound of designation red 3 (CDr3), developed through a high throughput/content screening of in-house generated diversity oriented fluorescence library in stem cells at different developmental stages. This novel compound specifically detects living neural stem cells of both human and mouse origin. Furthermore, we identified its binding target by proteomic analysis as fatty acid binding protein 7 (FABP7), also known as brain lipid binding protein) which is highly expressed in neural stem cells and localized in the cytoplasm. CDr3 will be a valuable chemical tool in the study and applications of neural stem cells.
Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore.
Seong-Wook
SW
Yun
Cheryl
C
Leong
Duanting
D
Zhai
Yee Ling
YL
Tan
Linda
L
Lim
Xuezhi
X
Bi
Jae-Jung
JJ
Lee
Han Jo
HJ
Kim
Nam-Young
NY
Kang
Shin Hui
SH
Ng
Lawrence W
LW
Stanton
Young-Tae
YT
Chang
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D049109:Cell Proliferation; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
D002352:Carrier Proteins; C107608:FABP7 protein, human; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005456:Fluorescent Dyes; D009419:Nerve Tissue Proteins; D025521:Tumor Suppressor Proteins