Synthetic mammalian riboswitches based on guanine aptazyme.
Allosteric hammerhead ribozymes (aptazymes) that are activated by guanine were used to control mammalian gene expression in cis and in trans. Coexpression of the two mechanistically distinct riboswitches resulted in an improved dynamic range of gene expression.
Department of Biomedical Engineering, University of California Davis, 451 Health Sciences Drive, Davis, CA 95616, USA.
Yoko
Y
Nomura
Deepak
D
Kumar
Yohei
Y
Yokobayashi
D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D016466:CHO Cells; D006224:Cricetinae; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000235:genetics; D006147:Guanine / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D016337:RNA, Catalytic / Q000235:genetics* / Q000378:metabolism; D058928:Riboswitch; D014162:Transfection
D052157:Aptamers, Nucleotide; D016337:RNA, Catalytic; D058928:Riboswitch; C485184:enhanced green fluorescent protein; C427204:hammerhead ribozyme; D049452:Green Fluorescent Proteins; D006147:Guanine