Crystallization of the novel S-adenosyl-L-methionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis.
Recombinant Q9F8T9 protein from Streptomyces rishiriensis (CouO), an S-adenosyl-L-methionine-dependent C-methyltransferase, has been successfully cloned, expressed and purified. CouO was crystallized from a single condition in the Morpheus crystallization screen. A vitrified crystal diffracted to 2.05 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 33.02, b = 82.87, c = 76.77 Å, β = 96.93°.
AAustrian Centre of Industrial Biotechnology, GmbH, Petersgasse 14, 8010 Graz, Austria. andrzej.lyskowski@acib.at
Andrzej
A
Lyskowski
Martin
M
Tengg
Georg
G
Steinkellner
Helmut
H
Schwab
Mandana
M
Gruber-Khadjawi
Karl
K
Gruber
D003460:Crystallization; D018360:Crystallography, X-Ray; D008780:Methyltransferases / Q000737:chemistry*; D013302:Streptomyces / Q000201:enzymology*
D008780:Methyltransferases; C093871:methionine S-methyltransferase