In vitro biochemical assays to monitor rhodopsin function.
Rhodopsin is the dim-light photoreceptor responsible for initiation of the visual transduction cascade. In the dark its activity is very low, while light activation catalyzes the activation of its G-protein transducin. The first step in resetting rhodopsin and the phototransduction cascade involves the phosphorylation of light-active rhodopsin by rhodopsin kinase. Here, we describe assays to monitor the function of rhodopsin or rhodopsin mutants.
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
Joshua
J
Sammons
Alecia K
AK
Gross
D000818:Animals; D019556:COS Cells; D002417:Cattle; D002522:Chlorocebus aethiops; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods; D051606:G-Protein-Coupled Receptor Kinase 1 / Q000378:metabolism; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods; D051379:Mice; D012243:Rhodopsin / Q000302:isolation & purification / Q000378:metabolism*; D015293:Transducin / Q000302:isolation & purification / Q000378:metabolism
D012243:Rhodopsin; D051606:G-Protein-Coupled Receptor Kinase 1; D015293:Transducin