[Relationship among the expression of lymphatic vessel density, microvessel density, carcinoembryonic antigenic mRNA, KAI1, and Kiss-1, and prognosis in patients with non-small cell lung cancer].
OBJECTIVE
In recent years, many studies have revealed the prognosis in patients with non-small cell lung cancer (NSCLC). In general, some clinic-pathological parameters have been related with prognosis. The aim of this study is to detect the relationship among lymphatic vessel density (LVD), microvessel density (MVD), expression of carcinoembryonic antigen (CEA) mRNA, metastasis suppressor genes (KAI1 and Kiss-1), and the prognosis of NSCLC patients.


METHODS
Blood samples were collected from 57 cases of NSCLC. The transcription of CEA mRNA was detected via nested reverse transcriptase-polymerase chain reaction and micro-fluid chip. Immunohistochemistry was performed to detect the expression of LVD, MVD, KAI1 and Kiss-1 in the patients. All follow-up data were collected and analyzed.


RESULTS
The overall five-year survival rate was 18%, and the median survival was 34 months. TNM stage, lymph node metastasis, and expression of MVD, LVD, CEA mRNA and Kiss-1 were factors to survival, as determined via single survival analysis. Multivariate analysis demonstrated that TNM stage, lymph node metastasis, and expression of CEA mRNA were independent prognostic factors for NSCLC patients.


CONCLUSIONS
The expression of MVD, LVD, Kiss-1 and CEA mRNA is related to the prognosis of NSCLC.
Department of Cardiothoracic Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
Guowen
G
Wang
Zuyi
Z
Wang
Chuankui
C
Li
Ping
P
Wang
Damin
D
Chai
Zenong
Z
Cheng
D002272:Carcinoembryonic Antigen / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000098:blood supply* / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D051861:Kangai-1 Protein / Q000235:genetics; D059648:Kisspeptins / Q000235:genetics; D008175:Lung Neoplasms / Q000098:blood supply* / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D042601:Lymphatic Vessels / Q000378:metabolism*; D055806:Microvessels / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D002272:Carcinoembryonic Antigen; C106628:KISS1 protein, human; D051861:Kangai-1 Protein; D059648:Kisspeptins; D012333:RNA, Messenger