Metagenomic microbial community profiling using unique clade-specific marker genes.
Metagenomic shotgun sequencing data can identify microbes populating a microbial community and their proportions, but existing taxonomic profiling methods are inefficient for increasingly large data sets. We present an approach that uses clade-specific marker genes to unambiguously assign reads to microbial clades more accurately and >50× faster than current approaches. We validated our metagenomic phylogenetic analysis tool, MetaPhlAn, on terabases of short reads and provide the largest metagenomic profiling to date of the human gut. It can be accessed at http://huttenhower.sph.harvard.edu/metaphlan/.
Department of Biostatistics, Harvard School of Public Health, Boston, Massachusetts, USA.
Nicola
N
Segata
Levi
L
Waldron
Annalisa
A
Ballarini
Vagheesh
V
Narasimhan
Olivier
O
Jousson
Curtis
C
Huttenhower
D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D056186:Metagenomics; D010802:Phylogeny; D015203:Reproducibility of Results
D005819:Genetic Markers