Sentulic acid: a cytotoxic ring A-seco triterpenoid from Sandoricum koetjape Merr.
A new ring A-seco triterpenoid, sentulic acid, along with a known oleanane-type triterpenoid, 3-oxoolean-12-en-27-oic acid, were isolated from the Indonesian plant Sandoricum koetjape Merr. Their chemical structures were elucidated by spectroscopic analysis. In addition, the cytotoxic effects of these compounds on human promyelocytic leukemia HL-60 cells were studied. The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays and trypan blue dye exclusion tests showed that sentulic acid and 3-oxoolean-12-en-27-oic acid were able to induce cytotoxicity in these cells. Furthermore, morphological examination and DNA fragmentation analysis indicated that these cytotoxic effects were mediated by apoptosis.
Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Andalas, 25163 Limau Manis, Padang, Indonesia. maiefdi@yahoo.com
Mai
M
Efdi
Masayuki
M
Ninomiya
Erma
E
Suryani
Kaori
K
Tanaka
Sanusi
S
Ibrahim
Kunitomo
K
Watanabe
Mamoru
M
Koketsu
D017209:Apoptosis / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D029582:Meliaceae / Q000737:chemistry*; D008968:Molecular Conformation; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity
D014315:Triterpenes; C575692:sentulic acid; D004247:DNA