Towards 3D modelling and imaging of infection scenarios at the single cell level using holographic optical tweezers and digital holographic microscopy.
The analysis of dynamic interactions of microorganisms with a host cell is of utmost importance for understanding infection processes. We present a biophotonic holographic workstation that allows optical manipulation of bacteria by holographic optical tweezers and simultaneously monitoring of dynamic processes with quantitative multi-focus phase imaging based on self-interference digital holographic microscopy. Our results show that several bacterial cells, even with non-spherical shape, can be aligned precisely on the surface of living host cells and localized reproducibly in three dimensions. In this way a new label-free multipurpose device for modelling and quantitative analysis of infection scenarios at the single cell level is provided.
Center for Biomedical Optics and Photonics, University of Muenster, Robert-Koch-Str. 45, 48149 Muenster, Germany. bkemper@uni-muenster.de
Björn
B
Kemper
Álvaro
Á
Barroso
Mike
M
Woerdemann
Lena
L
Dewenter
Angelika
A
Vollmer
Robin
R
Schubert
Alexander
A
Mellmann
Gert
G
von Bally
Cornelia
C
Denz
D001412:Bacillus subtilis / Q000166:cytology* / Q000502:physiology; D045744:Cell Line, Tumor; D006696:Holography / Q000379:methods*; D006801:Humans; D008853:Microscopy / Q000379:methods*; D008954:Models, Biological; D052898:Optical Tweezers; D059010:Single-Cell Analysis / Q000379:methods*