MicroRNA-100 regulates osteogenic differentiation of human adipose-derived mesenchymal stem cells by targeting BMPR2.
Elucidation of the molecular mechanisms governing human adipose-derived mesenchymal stem cells (hASCs) osteogenic differentiation is of great importance for improving the treatment of bone-related diseases. In this study, we examined the role of microRNA (miR)-100 on the osteogenesis of hASCs. Overexpression of miR-100 inhibited osteogenic differentiation of hASCs in vitro, whereas downregulation of miR-100 enhanced the process. Target prediction analysis and dual luciferase report assay confirmed that bone morphogenetic protein receptor type II (BMPR2) was a direct target of miR-100. Furthermore, knockdown of BMPR2 by RNA interference inhibited osteogenic differentiation of hASCs, similar as the effect of upregulation miR-100. Taken together, our findings imply that miR-100 plays a negative role in osteogenic differentiation and might act through targeting BMPR2.
Center of Excellence in Tissue Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Institute of Basic Medical Sciences and School of Basic Medicine, Beijing, People's Republic of China.
Yang
Y
Zeng
Xuebin
X
Qu
Hongling
H
Li
Shan
S
Huang
Shihua
S
Wang
Qilin
Q
Xu
Ruizhu
R
Lin
Qin
Q
Han
Jing
J
Li
Robert Chunhua
RC
Zhao
D000273:Adipose Tissue / Q000378:metabolism*; D000595:Amino Acid Sequence; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000378:metabolism*; D002454:Cell Differentiation; D015536:Down-Regulation; D017353:Gene Deletion; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D035683:MicroRNAs / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
C544266:MIRN100 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; C498218:BMPR2 protein, human; D052006:Bone Morphogenetic Protein Receptors, Type II