A simple and high resolution ion-pair HPLC method for separation and simultaneous determination of nitrate and thiocyanate in different water samples.
An ion-pair reversed-phase high-performance liquid chromatography method with isocratic elution and ultraviolet detection was developed and validated for the separation and simultaneous determination of nitrate and thiocyanate. The separation was performed on a C18 analytical column with mobile phase containing 0.08 mM hexadecyltrimethylammonium bromide as an ion-pair reagent, 40 mM of acetate buffer and 30% methanol at pH 3.2. The detection was monitored at 206 nm. The response was linear from 1 to 10 mM for nitrate, with a detection limit of 0.05 mM, and 1 to 10 mM for thiocyanate, with a detection limit of 0.31 mM. Calibration curves were found to be linear in these concentration ranges with correlation coefficient better than 0.99.
Department of Chemistry, Imam Khomeini International University, Qazvin, Iran. m-soleimani@hotmail.com
Majid
M
Soleimani
Yadollah
Y
Yamini
Farzane
F
Mohazab Rad