The structural biology of enzymes involved in natural product glycosylation.
The glycosylation of microbial natural products often dramatically influences the biological and/or pharmacological activities of the parental metabolite. Over the past decade, crystal structures of several enzymes involved in the biosynthesis and attachment of novel sugars found appended to natural products have emerged. In many cases, these studies have paved the way to a better understanding of the corresponding enzyme mechanism of action and have served as a starting point for engineering variant enzymes to facilitate to production of differentially-glycosylated natural products. This review specifically summarizes the structural studies of bacterial enzymes involved in biosynthesis of novel sugar nucleotides.
Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA.
Shanteri
S
Singh
George N
GN
Phillips
Jon S
JS
Thorson
D001419:Bacteria / Q000201:enzymology*; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D015394:Molecular Structure; D009711:Nucleotides / Q000378:metabolism; D011487:Protein Conformation
D001688:Biological Products; D009711:Nucleotides; D016695:Glycosyltransferases