Crystallization and preliminary X-ray diffraction analysis of the SOD-TAT fusion protein.
The superoxide dismutase (SOD) family of proteins are necessary to protect oxygen-utilizing cells from the toxicity of reactive oxygen species. The delivery of SOD into tissues is severely limited by its size and biochemical properties. A cell-membrane-permeable SOD, SOD-TAT, has been demonstrated to have the ability to be directly transduced into mammalian cells. In this study, the SOD-TAT fusion protein was expressed, purified and crystallized. Crystals of the SOD-TAT fusion protein diffracted to 3.20 Å resolution and belonged to space group C121.
College of Bioscience and Biotechnology, Fuzhou University, 2 Xue Yuan Road, Fuzhou, Fujian 350108, People's Republic of China.
Jianru
J
Pan
Kangjing
K
Zhou
Guangjin
G
Zheng
Shutao
S
Liu
Pingfan
P
Rao
D003460:Crystallization; D018360:Crystallography, X-Ray; D015696:Gene Products, tat / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D013482:Superoxide Dismutase / Q000737:chemistry*
D015696:Gene Products, tat; D011994:Recombinant Proteins; D013482:Superoxide Dismutase