The simian varicella virus ORF A is expressed in infected cells but is non-essential for replication in cell culture.
The simian varicella virus (SVV) genome encodes ORF A, a truncated homolog of SVV ORF 4. The SVV ORF A was expressed as a 1.0-kb transcript in SVV-infected Vero cells. The ORF A promoter was active in infected Vero cells and was stimulated by the SVV immediate-early gene ORF 62 product (IE62), a viral transactivator of SVV genes. The SVV ORF A did not transactivate SVV IE, early, or late gene promoters in transfected Vero cells and was unable to augment IE62-mediated transactivation of SVV promoters. A SVV mutant lacking ORF A replicated as efficiently as wild-type SVV in infected Vero cells, indicating that ORF A expression is not essential for in vitro replication.
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, 4301 West Markham Street, Slot 511, Little Rock, AR 72205, USA. graywaynel@uams.edu
Wayne L
WL
Gray
D000818:Animals; D002522:Chlorocebus aethiops; D017353:Gene Deletion; D015870:Gene Expression; D020869:Gene Expression Profiling; D018140:Varicellovirus / Q000235:genetics / Q000502:physiology*; D014709:Vero Cells; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics; D014779:Virus Replication
D014764:Viral Proteins