Biological, immunological and regenerative characteristics of placenta-derived mesenchymal stem cell isolated using a time-gradient attachment method.
It has been verified that placenta contains multi-lineage mesenchymal stem cells (MSCs). We have used a time-gradient attachment method to isolate placenta-derived MSCs (PMSCs). The morphology, differentiation potential, immunogenicity and xenogenic reconstruction potential of these PMSCs were examined. The results showed that PMSCs isolated using the time-gradient attachment method showed higher potential of in vitro proliferation and multi-lineage differentiation. PMSCs isolated using the time-gradient attachment method showed a low immunogenicity. HLA-A gene fragment and no HLA-DR gene fragment were detected in PMSCs isolated using the time-gradient attachment method, and the mixed lymphocyte reaction (MLR) assay identified that these cells inhibited the proliferation of the allogeneic T-lymphocytes induced by PHA. The transplantation in calvaria of rats showed that PMSCs had the higher xenogenic reconstruction potential. Finally, the significance of PMSCs isolated using the time-gradient attachment method in experimental and clinical applications is discussed.
Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Wenji
W
Yuan
Chen
C
Zong
Yingzhi
Y
Huang
Ying
Y
Gao
Dongyan
D
Shi
Changsong
C
Chen
Liyue
L
Liu
Jinfu
J
Wang
D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D048430:Cell Shape; D005260:Female; D006801:Humans; D056747:Immunomodulation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology*; D010641:Phenotype; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D012886:Skull / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing