[Study of expression of cytokine genes in the process of cultivation of healthy donor leukocytes].
OBJECTIVE
Study of expression of cytokine genes in the process of cultivation of healthy donor leukocytes.


METHODS
RNA isolated before, after 3 and 24 hours of cultivation of leukocytes of 15 healthy donors aged 19 - 32 years was the object of the study. Study of the expression of genes of 8 cytokines by the level of their mRNA was performed by using polymerase chain reactionwith reverse transcription.


RESULTS
In healthy donors among with individual differences, general regularities were traced: higher levels of IFNalpha and anti-inflammatory cytokine gene expression, as well as predominantly low expression of pro-inflammatory cytokines.


CONCLUSIONS
In most of the cases in the process of cultivation of leukocytes isolated from blood donors the highest level of gene expression of pro-inflammatory cytokines is noted after 3 hours of cultivation, and expression of genes of anti-inflammatory cytokines remains constant for 24 hours of cultivation.
L V
LV
Koval'chuk
L V
LV
Gankovskaia
M V
MV
Mezentseva
I M
IM
Shapoval
Iu V
IuV
Aitova
A M
AM
Magomedova
V V
VV
Romanovskaia
D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D013997:Time Factors
D016207:Cytokines; D012333:RNA, Messenger