A neuronal transmembrane protein LRFN4 complexes with 14-3-3s and NCK1 to induce morphological change in monocytic cells via Rac1-mediated actin cytoskeleton reorganization.
We previously reported that leucine-rich repeat and fibronectin type III domain-containing 4 (LRFN4) functioned in migration and morphological change (i.e. cell elongation) of monocytic cells. Here, we examined a molecular mechanism regulating LRFN4-mediated cell elongation. We found that 14-3-3 and NCK proteins complexed with LRFN4, and they were involved in LRFN4-mediated cell elongation. We also identified the regions of LRFN4 interacting with NCK1 and 14-3-3s. Finally, we demonstrated that a Rac1 small GTPase was involved in LRFN4-mediated cell elongation. These results indicated that LRFN4 complexed with 14-3-3s and NCK1 to mediate elongation in monocytic cells via Rac-1-mediated actin cytoskeleton reorganization.
Department of Medicinal and Life Science, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. s-konaka@rs.noda.tus.ac.jp
Shu
S
Konakahara
Yuki
Y
Suzuki
Takao
T
Kawakami
Maki
M
Saitou
Masunori
M
Kajikawa
Yasuhiko
Y
Masuho
Junya
J
Kohroki
D048948:14-3-3 Proteins / Q000737:chemistry* / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D009000:Monocytes / Q000166:cytology; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D015513:Oncogene Proteins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D020033:Protein Isoforms; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*
D048948:14-3-3 Proteins; D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; C561460:LRFN4 protein, human; D008562:Membrane Glycoproteins; C078332:Nck protein; D009419:Nerve Tissue Proteins; D015513:Oncogene Proteins; D020033:Protein Isoforms; C107953:RAC1 protein, human; D020830:rac1 GTP-Binding Protein