Recombinant canine distemper virus serves as bivalent live vaccine against rabies and canine distemper.
Effective, safe, and affordable rabies vaccines are still being sought. Attenuated live vaccine has been widely used to protect carnivores from canine distemper. In this study, we generated a recombinant canine distemper virus (CDV) vaccine strain, rCDV-RVG, expressing the rabies virus glycoprotein (RVG) by using reverse genetics. The recombinant virus rCDV-RVG retained growth properties similar to those of vector CDV in Vero cell culture. Animal studies demonstrated that rCDV-RVG was safe in mice and dogs. Mice inoculated intracerebrally or intramuscularly with rCDV-RVG showed no apparent signs of disease and developed a strong rabies virus (RABV) neutralizing antibody response, which completely protected mice from challenge with a lethal dose of street virus. Canine studies showed that vaccination with rCDV-RVG induced strong and long-lasting virus neutralizing antibody responses to RABV and CDV. This is the first study demonstrating that recombinant CDV has the potential to serve as bivalent live vaccine against rabies and canine distemper in animals.
Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Xijun
X
Wang
Na
N
Feng
Jinying
J
Ge
Lei
L
Shuai
Liyan
L
Peng
Yuwei
Y
Gao
Songtao
S
Yang
Xianzhu
X
Xia
Zhigao
Z
Bu
D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D002522:Chlorocebus aethiops; D004216:Distemper / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D004217:Distemper Virus, Canine / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D004285:Dogs; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology*; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D011818:Rabies / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D011820:Rabies virus / Q000276:immunology* / Q000472:pathogenicity; D059386:Reverse Genetics / Q000379:methods; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014779:Virus Replication
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus