Complementary surface-enhanced resonance Raman spectroscopic biodetection of mixed protein solutions by chitosan- and silica-coated plasmon-tuned silver nanoparticles.
Silver nanoparticles with identical plasmonic properties but different surface functionalities are synthesized and tested as chemically selective surface-enhanced resonance Raman (SERR) amplifiers in a two-component protein solution. The surface plasmon resonances of the particles are tuned to 413 nm to match the molecular resonance of protein heme cofactors. Biocompatible functionalization of the nanoparticles with a thin film of chitosan yields selective SERR enhancement of the anionic protein cytochrome b(5), whereas functionalization with SiO(2) amplifies only the spectra of the cationic protein cytochrome c. As a result, subsequent addition of the two differently functionalized particles yields complementary information on the same mixed protein sample solution. Finally, the applicability of chitosan-coated Ag nanoparticles for protein separation was tested by in situ resonance Raman spectroscopy.
Institut für Chemie, Technische Universität Berlin, Berlin, Germany.
Arumugam
A
Sivanesan
Govindasamy
G
Kalaivani
Anna
A
Fischer
Konstanze
K
Stiba
Silke
S
Leimkühler
Inez M
IM
Weidinger
D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D003574:Cytochrome c Group / Q000032:analysis*; D015786:Cytochromes b5 / Q000032:analysis*; D006736:Horses; D006801:Humans; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry*; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*
D003574:Cytochrome c Group; D012834:Silver; D012822:Silicon Dioxide; D048271:Chitosan; D015786:Cytochromes b5