α-Actinin4 nuclear translocation mediates gonadotropin-releasing hormone stimulation of follicle-stimulating hormone β-subunit gene transcription in LβT2 cells.
Gonadotropin-releasing hormone (GnRH) regulates the synthesis and secretion of follicle-stimulating hormone (FSH) by stimulating the transcription of Fshβ gene. Our iTRAQ quantitative proteomics result showed that the abundance of α-actinin4 (ACTN4) increased in the nuclei of LβT2 cells upon GnRH induction. Using RNA interference, reverse transcription and real-time PCR, luciferase and transient transfection assays, we proved that ACTN4 is involved in the regulation of mouse Fshβ gene (mFshβ) transcription and its C-terminal calmodulin (CaM)-like domain is crucial for this process. Our study suggests that ACTN4 nuclear translocation mediates GnRH stimulation of mFshβ gene transcription.
Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.
Han
H
Yu
Zhengjun
Z
Li
Dipanjana
D
Ghosh
Teck Kwang
TK
Lim
Yuehui
Y
He
Qingsong
Q
Lin
D000185:Actinin / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D017931:DNA Primers; D037201:Follicle Stimulating Hormone, beta Subunit / Q000235:genetics*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D051379:Mice; D011401:Promoter Regions, Genetic; D021381:Protein Transport; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects*
C491752:Actn4 protein, mouse; D017931:DNA Primers; D037201:Follicle Stimulating Hormone, beta Subunit; D000185:Actinin; D007987:Gonadotropin-Releasing Hormone