Evaluation of platelet function during extended storage in additive solution, prepared in a new container that allows manual buffy-coat platelet pooling and leucoreduction in the same system.
BACKGROUND
A novel and practical storage container designed for manual buffy-coat pooling and leucodepletion was evaluated to assess its filtration performance and to analyse the quality of stored leucoreduced buffy-coat-derived platelet pools.


METHODS
To analyse the grifols leucored transfer PL system, blood was collected from random donors into standard triple bag systems, and fractionated using standard procedures to obtain buffy-coats. Ten leucodepleted platelet pools were prepared each from five units of buffy-coats in additive solution. Concentrates were stored for 10 days at 22 °C on an end-over-end agitator. On days 0, 5, 7, and 10 of storage, samples were tested using standard in vitro platelet parameters.


RESULTS
The use of this novel system for volume reduction and leucodepletion of buffy-coats resuspended in additive solution led to platelet pools that met the European requirements. pH was maintained well, declining from an initial value of 7.11±0.04 to 6.88±0.08 after 10 days. Parameters of cell lysis, response to a hypotonic stimulus and aggregation induced by agonists (arachidonic acid, ristocetin, collagen or thrombin receptor activating peptide) were also well-preserved. During storage, the quality profile of the platelet pools remained very similar to that previously reported in platelet concentrates in terms of metabolism, platelet activation (CD62, CD63, sCD62), expression of glycoproteins Ib and IIb/IIIa, capacity of glycoprotein IIb/IIIa to become activated upon ADP stimulation, and release of biological response modifiers (sCD40L and RANTES).


CONCLUSIONS
This new system allows the preparation of leucodepleted buffy-coat platelet pools in additive solution with good preservation of platelet function. The logistics of the procedure are relatively simple and it results in good-quality components, which may reduce costs and ease the process of buffy-coat pooling and leucocyte reduction in transfusion services.
Regional Blood Donation Centre and University of Murcia, Spain.
Eva María
EM
Plaza
María Luisa
ML
Lozano
Isabel Sánchez
IS
Guiu
José Manuel
JM
Egea
Vicente
V
Vicente
Laura Collantes
LC
De Terán
José
J
Rivera
D016824:Antigens, Human Platelet / Q000096:biosynthesis*; D058621:Blood Buffy Coat / Q000166:cytology / Q000378:metabolism; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D001793:Blood Preservation; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D047589:Leukocyte Reduction Procedures / Q000295:instrumentation / Q000379:methods; D008297:Male; D010979:Platelet Function Tests / Q000379:methods; D013997:Time Factors
D016824:Antigens, Human Platelet