Carbohydrate recognition mechanism of HA70 from Clostridium botulinum deduced from X-ray structures in complexes with sialylated oligosaccharides.
Clostridium botulinum produces the botulinum neurotoxin, forming a large complex as progenitor toxins in association with non-toxic non-hemagglutinin and/or several different hemagglutinin (HA) subcomponents, HA33, HA17 and HA70, which bind to carbohydrate of glycoproteins from epithelial cells in the infection process. To elucidate the carbohydrate recognition mechanism of HA70, X-ray structures of HA70 from type C toxin (HA70/C) in complexes with sialylated oligosaccharides were determined, and a binding assay by the glycoconjugate microarray was performed. These results suggested that HA70/C can recognize both α2-3- and α2-6-sialylated oligosaccharides, and that it has a higher affinity for α2-3-sialylated oligosaccharides.
Life Science Research Center and Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Kita-gun, Kagawa 761-0793, Japan.
Satoshi
S
Yamashita
Hiromi
H
Yoshida
Noboru
N
Uchiyama
Yukari
Y
Nakakita
Shin-ichi
S
Nakakita
Takashi
T
Tonozuka
Keiji
K
Oguma
Atsushi
A
Nishikawa
Shigehiro
S
Kamitori
D001905:Botulinum Toxins / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D003014:Clostridium botulinum / Q000378:metabolism*; D018360:Crystallography, X-Ray / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000378:metabolism; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D006388:Hemagglutinins / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011485:Protein Binding; D013696:Temperature
D002241:Carbohydrates; D006023:Glycoproteins; D006388:Hemagglutinins; D009844:Oligosaccharides; D001905:Botulinum Toxins; C096324:botulinum toxin type C; D019158:N-Acetylneuraminic Acid; D005947:Glucose; D005690:Galactose