Effect of S-PRG Eluate on Biofilm Formation and Enzyme Activity of Oral Bacteria.
Recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (S-PRG) filler was revealed. We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis. Adherence ability of S. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of P. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. Coaggregation of P. gingivalis with Fusobacterium nucleatum was also examined. S-PRG eluate was found to suppress streptococcal adherence. S-PRG eluate inhibited the protease and gelatinase activities of P. gingivalis and the coaggregation between P. gingivalis and F. nucleatum. These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis. These findings may prompt research into novel strategies for preventing caries and periodontitis.
Center for Oral Diseases, Fukuoka Dental College, 3-2-1 Hakataekimae, Hakata-ku 812-0011, Japan.
Masahiro
M
Yoneda
Nao
N
Suzuki
Yosuke
Y
Masuo
Akie
A
Fujimoto
Kosaku
K
Iha
Kazuhiko
K
Yamada
Tomoyuki
T
Iwamoto
Takao
T
Hirofuji