The SUMO protease Verloren regulates dendrite and axon targeting in olfactory projection neurons.
Sumoylation is a post-translational modification regulating numerous biological processes. Small ubiquitin-like modifier (SUMO) proteases are required for the maturation and deconjugation of SUMO proteins, thereby either promoting or reverting sumoylation to modify protein function. Here, we show a novel role for a predicted SUMO protease, Verloren (Velo), during projection neuron (PN) target selection in the Drosophila olfactory system. PNs target their dendrites to specific glomeruli within the antennal lobe (AL) and their axons stereotypically into higher brain centers. We uncovered mutations in velo that disrupt PN targeting specificity. PN dendrites that normally target to a particular dorsolateral glomerulus instead mistarget to incorrect glomeruli within the AL or to brain regions outside the AL. velo mutant axons also display defects in arborization. These phenotypes are rescued by postmitotic expression of Velo in PNs but not by a catalytic domain mutant of Velo. Two other SUMO proteases, DmUlp1 and CG12717, can partially compensate for the function of Velo in PN dendrite targeting. Additionally, mutations in SUMO and lesswright (which encodes a SUMO conjugating enzyme) similarly disrupt PN targeting, confirming that sumoylation is required for neuronal target selection. Finally, genetic interaction studies suggest that Velo acts in SUMO deconjugation rather than in maturation. Our study provides the first in vivo evidence for a specific role of a SUMO protease during neuronal target selection that can be dissociated from its functions in neuronal proliferation and survival.
Howard Hughes Medical Institute, Department of Biology, Stanford University, Stanford, California 94305, USA.
Daniela
D
Berdnik
Vincenzo
V
Favaloro
Liqun
L
Luo
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000502:physiology*; D001921:Brain / Q000254:growth & development / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism / Q000502:physiology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D009154:Mutation / Q000502:physiology; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology*; D009833:Olfactory Pathways / Q000254:growth & development*; D011499:Protein Processing, Post-Translational; D025841:Small Ubiquitin-Related Modifier Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D058207:Sumoylation / Q000502:physiology
D029721:Drosophila Proteins; D025841:Small Ubiquitin-Related Modifier Proteins