Histamine determination in human urine using sub-2 μm C18 column with fluorescence and mass spectrometric detection.
A fast, sensitive, and selective method for the determination of histamine in human urine samples by ultrahigh pressure liquid chromatography (LC) with fluorescence and mass spectrometry (MS) detection is investigated. A fluorescent reagent, 4-(1-pyrene) butyric acid N-hydroxysuccinimide ester was conjugated to the primary and secondary amino moieties of histamine. The structure of dipyrene-labeled histamine in human urine was determined by quadrupole time-of-flight MS with electospray ionization interface. The determination of the dipyrene derivative of histamine in urine samples was achieved within 3.9 min on an ultrahigh pressure LC Eclipse Zorbax XDB-C(18)  column with 1.8 μm particle diameter. In this work, histamine separation was achieved significantly faster (3.9 min) with improved detection limit (signal-to-noise = 3) of 0.04 nM than 19.5 min with a detection limit of 0.183 nM as reported in a previous method.
Innovative Chromatography Group, The Irish Separation Science Cluster, Department of Chemistry and the Analytical & Biological Chemistry Research Facility, University College Cork, Cork, Ireland.
Anna-Marie
AM
Hogan
Conor
C
Crean
Una Marie
UM
Barrett
Elizabeth
E
Guihen
Jeremy D
JD
Glennon
D006632:Histamine / Q000652:urine*; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity
D006632:Histamine