[Clinical and experimental study of a multiple myeloma case with low hypodiploidy].
OBJECTIVE
To report the clinical and laboratory characterization of a case of multiple myeloma with low hypodiploid complex karyotyptic abnormalities.


METHODS
Cytogenetic examination of bone marrow performed by 24 h culture method. R-banding technique was used to analyze the karyotype. Interphase fluorescence in situ hybridization (FISH) was performed using chromosome probes such as 13q14, p53, Rb1, 1q21 and IgH/CCND1. The DNA content was detected by flow cytometry.


RESULTS
Chromosome analysis revealed complex chromosomal rearrangement. Five cells had a low hypodiploid karyotype with 35 chromosomes. Three cells had the duplication of the low hypodiploid karyotype. Four cells had a normal karyotype. Monosomy 1, 13, 14, 17 and a mark chromosome 1 derived from chromosome 11 resulting in the amplication of CCND1 gene were confirmed by interphase FISH. Flow cytometric analysis displayed a low hypodiploid peak with the DNA index of 0.8426.


CONCLUSIONS
These results indicated that the low hypodiploidy is a rare abnormality in multiple myeloma. Interphase FISH is a reliable method for detecting molecular abnormalities in multiple myeloma.
The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Thrombosis and Hemostasis, Key Laboratory of the Ministry of Health, Suzhou, Jiangsu 215006, P. R. China.
Shu-xiao
SX
Bai
Jin-lan
JL
Pan
Yong-quan
YQ
Xue
Su-ning
SN
Chen
Ya-fang
YF
Wu
Yong
Y
Wang
Jun
J
Zhang
Juan
J
Shen
D059786:Abnormal Karyotype; D000328:Adult; D003582:Cytogenetics / Q000379:methods; D005260:Female; D015321:Gene Rearrangement / Q000235:genetics; D006801:Humans; D009101:Multiple Myeloma / Q000175:diagnosis / Q000235:genetics*