Three-dimensional in vivo imaging of tumors expressing red fluorescent proteins.
3D imaging of genetically-engineered fluorescent tumors enables quantitative monitoring of tumor growth/regression, metastatic processes, including during anticancer therapy in real-time.Fluorescent tumor models for 3D imaging require stable expression of genetically encoded fluorescent proteins and maintenance of the properties of tumor cell line including growth rate, morphology, and immunophenotype.In this chapter, the protocol for 3D imaging of tumors expressing red fluorescent protein are described in detail.
A.N. Bach Institute of Biochemistry of Russian Academy of Sciences, Moscow, Russia. apsavitsky@inbi.ras.ru
Alexander P
AP
Savitsky
Irina G
IG
Meerovich
Victoria V
VV
Zherdeva
Lyaysan R
LR
Arslanbaeva
Olga S
OS
Burova
Darina V
DV
Sokolova
Elena M
EM
Treshchalina
Anatoly Yu
AY
Baryshnikov
Ilya I
II
Fiks
Anna G
AG
Orlova
Michael S
MS
Kleshnin
Ilya V
IV
Turchin
Alexander M
AM
Sergeev
D000818:Animals; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein