Fungal fludioxonil sensitivity is diminished by a constitutively active form of the group III histidine kinase.
The fungicide fludioxonil is used to control plant-pathogenic fungi by causing improper activation of the Hog1-type MAPK. However, the appearance of fludioxonil resistant mutants, mostly caused by mutations in the group III histidine kinases, poses a serious problem. Moreover, such mutations cause also hyperosmotic sensitivity and the underlying mechanism has been elusive for a long time. Using Saccharomyces cerevisiae as an experimental host, we show that those phenotypes are conferred by a constitutively active form of the group III histidine kinase. Our results explain the different reasons for fludioxonil resistance conferred by its deletion and missense mutation.
Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, 40530 Gothenburg, Sweden. kentaro.furukawa@cmb.gu.se
Kentaro
K
Furukawa
Anmoldeep
A
Randhawa
Harsimran
H
Kaur
Alok K
AK
Mondal
Stefan
S
Hohmann
D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology*; D001671:Biochemistry / Q000379:methods; D004149:Dioxoles / Q000494:pharmacology*; D025141:Drug Resistance, Fungal; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D000071677:Histidine Kinase; D008969:Molecular Sequence Data; D009154:Mutation; D020125:Mutation, Missense; D010641:Phenotype; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011758:Pyrroles / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D000935:Antifungal Agents; D004149:Dioxoles; D011758:Pyrroles; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C106494:HSL1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; D000071677:Histidine Kinase; C108339:fludioxonil