Nicotiana tabacum Tsip1-interacting ferredoxin 1 affects biotic and abiotic stress resistance.
Tsip1, a Zn finger protein that was isolated as a direct interactor with tobacco stress-induced 1 (Tsi1), plays an important role in both biotic and abiotic stress signaling. To further understand Tsip1 function, we searched for more Tsip1-interacting proteins by yeast two-hybrid screening using a tobacco cDNA library. Screening identified a new Tsip1-interacting protein, Nicotiana tabacum Tsip1-interacting ferredoxin 1 (NtTfd1), and binding specificity was confirmed both in vitro and in vivo. The four repeats of a cysteine-rich motif (CXXCXGXG) of Tsip1 proved important for binding to NtTfd1. Virus-induced gene silencing of NtTfd1, Tsip1, and NtTfd1/Tsip1 rendered plants more susceptible to salinity stress compared with TRV2 control plants. NtTfd1- and Tsip1-silenced tobacco plants were more susceptible to infection by Cucumber mosaic virus compared with control plants. These results suggest that NtTfd1 might be involved in the regulation of biotic and abiotic stresses in chloroplasts by interaction with Tsip1.
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Sung Un
SU
Huh
In-Ju
IJ
Lee
Byung-Kook
BK
Ham
Kyung-Hee
KH
Paek
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D002734:Chlorophyll / Q000378:metabolism; D017799:Cucumovirus / Q000502:physiology; D060467:Disease Resistance / Q000235:genetics; D005288:Ferredoxins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D008969:Molecular Sequence Data; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D055049:Salt Tolerance / Q000235:genetics; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
D005288:Ferredoxins; D010940:Plant Proteins; D002734:Chlorophyll