Impaired bone formation with a high-protein diet in rats with adriamycin-induced nephrotic syndrome.
OBJECTIVE
The purpose of the study was to investigate the effects of a high-protein (HP) diet on bone metabolism in rats with adriamycin (ADR)-induced nephrotic syndrome.


METHODS
Nephrotic syndrome was established by weekly injections of ADR (2 mg/kg, i.p.) for 6 weeks. After a final injection, we confirmed that nephrotic syndrome had developed. Then, the rats were divided into two groups for the dietary treatments, namely the HP diet (30% of calories from protein) and the low-protein (LP) diet (7% of calories from protein), and were fed an isocaloric diet for the following 5 weeks.


RESULTS
Urinary protein and phosphate excretion were significantly greater in the HP diet group than in the LP diet group (p < 0.05). Serum parathyroid hormone and osteocalcin levels were significantly higher and lower, respectively, in the HP diet group (p < 0.05). Femur weight, femur mass index and femur calcium contents were significantly lower in the HP diet group than in the LP diet group (p < 0.05). Bone mineral density was significantly lower in the HP diet group than in the LP diet group (p < 0.05); however, bone mineral content did not differ between the two groups.


CONCLUSIONS
We confirmed that an HP diet negatively affects bone mineral metabolism and bone density in ADR-induced nephrotic syndrome rats.
Department of Medical Nutrition, Kyung Hee University, Yongin, Korea.
A Young
AY
Lim
Su Yeon
SY
Kim
In Seok
IS
Lee
Ryowon
R
Choue
D000818:Animals; D015519:Bone Density / Q000187:drug effects* / Q000502:physiology; D002113:Calcification, Physiologic / Q000187:drug effects* / Q000502:physiology; D004044:Dietary Proteins / Q000008:administration & dosage / Q000633:toxicity*; D004317:Doxorubicin / Q000633:toxicity*; D008297:Male; D009404:Nephrotic Syndrome / Q000139:chemically induced* / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D004044:Dietary Proteins; D004317:Doxorubicin