Multicolor super-resolution DNA imaging for genetic analysis.
Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100 bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
Murat
M
Baday
Aaron
A
Cravens
Alex
A
Hastie
Hyeongjun
H
Kim
Deren E
DE
Kudeki
Pui-Yan
PY
Kwok
Ming
M
Xiao
Paul R
PR
Selvin
D010582:Bacteriophage lambda / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D014644:Genetic Variation / Q000235:genetics; D006801:Humans
D004247:DNA; D004720:Endonucleases