Trichoderma mienum sp. nov., isolated from mushroom farms in Japan.
During an investigation of Hypocrea/Trichoderma species inhabiting mushroom bedlogs, we found five strains of an undescribed species from a culture collection. These were analyzed using a combined approach, including morphology of holomorph, cultural studies, and phylogenetic analyses of the rRNA gene cluster of the internal transcribed spacer region, translation elongation factor 1-α, and RNA polymerase subunit II gene sequences. Distinctive morphological characters include stromata with green ascospores produced on potato dextrose agar medium, and Gliocladium-like to irregularly Verticillium-like conidiophores. In phylogenetic analyses, this species belongs to the Semiorbis clade, but its morphological characteristics do not match the other members of this clade. Based on morphological observations and phylogenetic analyses, we describe this as a new species, Trichoderma mienum, representing its Hypocrea teleomorph and Trichoderma anamorph.
The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-cho-minami, Tottori, 680-8553, Japan.
Chang Sun
CS
Kim
Takashi
T
Shirouzu
Akira
A
Nakagiri
Kozue
K
Sotome
Eiji
E
Nagasawa
Nitaro
N
Maekawa
D000363:Agaricales / Q000254:growth & development*; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D007564:Japan; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000166:cytology; D014242:Trichoderma / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*
D003470:Culture Media; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D020648:Peptide Elongation Factor 1; D012321:DNA-Directed RNA Polymerases