[Determination of lutein and zeaxanthin in serum by high performance liquid chromatography].
OBJECTIVE
To establish a simultaneous determination method for measuring lutein, zeaxanthin and β-carotene in serum by internal standard on C(30)-HPLC.


METHODS
Experimental data were as follows: stationary phase, Develosil carotenoid column C(30) (250 mm×4.6 mm, 5 μm); mobile phase A,  acetonitrile:methanol (3:2, v/v); mobile phase B, MTBE; grads elution; flow rate, 1 mL/min; monitoring wavelength, 450 nm; injection volume, 20 μL;  column temperature, 25 °C.


RESULTS
Lutein, zeaxanthin and β-carotene were thoroughly separated with the average retention time of 9.9 min, 10.3 min  and 21.2 min, respectively. The intra-day relative standard deviation (RSD) values were 3.22%, 3.81% and 1.60%. The linear ranges of serum  concentrations of lutein and β-carotene were both 0.012 5-12.5 mg/L (r=0.999 5, r=0.999 7), and that of zeaxanthin was 0.005-5.0 mg/L (r=1). The mean serum  concentrations of lutein, zeaxanthin and β-carotene for 58 healthy elder inhabitants (>50 years) were 0.410 μmol/L, 0.054 μmol/L and 0.128 μmol/L,  respectively.


CONCLUSIONS
This established method can be used for determination of lutein, zeaxanthin and β-carotene in serum.
Department of Nutrition and Food Hygiene, Peking University School of Public Health, Beijing, China.
Yang-mu
YM
Huang
Shao-fen
SF
Yan
Le
L
Ma
Zhi-yong
ZY
Zou
Xian-rong
XR
Xu
Xin
X
Xiao
Xun
X
Wang
Fei-fei
FF
Huang
Xiao-ming
XM
Lin
D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D014975:Lutein / Q000097:blood*; D024341:Xanthophylls / Q000097:blood*; D065146:Zeaxanthins; D019207:beta Carotene / Q000097:blood
D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D014975:Lutein