QuaMeter: multivendor performance metrics for LC-MS/MS proteomics instrumentation.
LC-MS/MS-based proteomics studies rely on stable analytical system performance that can be evaluated by objective criteria. The National Institute of Standards and Technology (NIST) introduced the MSQC software to compute diverse metrics from experimental LC-MS/MS data, enabling quality analysis and quality control (QA/QC) of proteomics instrumentation. In practice, however, several attributes of the MSQC software prevent its use for routine instrument monitoring. Here, we present QuaMeter, an open-source tool that improves MSQC in several aspects. QuaMeter can directly read raw data from instruments manufactured by different vendors. The software can work with a wide variety of peptide identification software for improved reliability and flexibility. Finally, QC metrics implemented in QuaMeter are rigorously defined and tested. The source code and binary versions of QuaMeter are available under Apache 2.0 License at http://fenchurch.mc.vanderbilt.edu.
Department of Biomedical Informatics, Vanderbilt University Medical Center, Nashville, Tennessee 37232-8575, United States.
Ze-Qiang
ZQ
Ma
Kenneth O
KO
Polzin
Surendra
S
Dasari
Matthew C
MC
Chambers
Birgit
B
Schilling
Bradford W
BW
Gibson
Bao Q
BQ
Tran
Lorenzo
L
Vega-Montoto
Daniel C
DC
Liebler
David L
DL
Tabb
D002853:Chromatography, Liquid / Q000295:instrumentation*; D010455:Peptides / Q000032:analysis; D040901:Proteomics / Q000295:instrumentation*; D012984:Software; D053719:Tandem Mass Spectrometry / Q000295:instrumentation*; D013997:Time Factors
D010455:Peptides