DNA analysis by application of Pt nanoparticle electrochemical amplification with single label response.
This study demonstrates a highly sensitive sensing scheme for the detection of low concentrations of DNA, in principle down to the single biomolecule level. The previously developed technique of electrochemical current amplification for detection of single nanoparticle (NP) collisions at an ultramicroelectrode (UME) has been employed to determine DNA. The Pt NP/Au UME/hydrazine oxidation reaction was employed, and individual NP collision events were monitored. The Pt NP was modified with a 20-base oligonucleotide with a C6 spacer thiol (detection probe), and the Au UME was modified with a 16-base oligonucleotide with a C6 spacer thiol (capture probe). The presence of a target oligonucleotide (31 base) that hybridized with both capture and detection probes brought a Pt NP on the electrode surface, where the resulting electrochemical oxidation of hydrazine resulted in a current response.
Department of Chemistry, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 143-701, Korea.
Seong Jung
SJ
Kwon
Allen J
AJ
Bard
D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008839:Microelectrodes; D010984:Platinum / Q000737:chemistry
D010984:Platinum; D006046:Gold; D004247:DNA