Adhesion molecule L1 is down-regulated in malignant peripheral nerve sheath tumors versus benign neurofibromatosis type 1-associated tumors.
Type 1 neurofibromatosis (NF-1), also known as von Recklinghausen disease, is caused by a disorder of a single gene on chromosome 17 that usually restrains cell division. A sequence that is frequently associated with NF-1 is tumor progression from neurofibromas to malignant peripheral nerve sheath tumors (MPNSTs). The aim of this study was to determine the expression of the neural L1 cell adhesion molecule in dermal-diffuse neurofibromas, plexiform neurofibromas, and MPNSTs of NF-1. We retrospectively analyzed surgically resected primary tumors, including 20 dermal neurofibromas, 23 plexiform neurofibromas, and 17 MPNSTs, by immunohistochemistry in paraffin sections of NF-1 tumors with the use of the L1-specific monoclonal antibody UJ127, which does not cross-react with other members of the L1 family. Immunostainings for CD34 and S100 were included to distinguish and allocate L1-expressing Schwann cells and perineural (specialized) fibroblasts. Our data showed that L1 is highly expressed in all benign NF-1 tumors and in some but not all MPNSTs. Furthermore, we demonstrated a correlation between L1 expression and differentiation grade of MPNSTs. There was a significant trend toward lower or nondetectable expression in the poorly differentiated MPNSTs, in contrast to all other tumor entities so far investigated, in which L1 expression correlated positive with malignancy, except for juvenile but not adult-derived neuroblastomas. Future studies are warranted to elucidate the molecular basis of the varying effects of the degree of L1 expression, receptor, and signal transduction mechanisms in different tumors.
Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Germany. blessmann@uke.uni-hamburg.de
Marco
M
Blessmann
Alexander
A
Gröbe
Alexander
A
Quaas
Jussuf T
JT
Kaifi
Georgios
G
Mistakidis
Christian
C
Bernreuther
Guido
G
Sauter
Stephanie
S
Gros
Tamina
T
Rawnaq
Reinhard
R
Friedrich
Victor F
VF
Mautner
Ralf
R
Smeets
Max
M
Heiland
Melitta
M
Schachner
Jakob R
JR
Izbicki
D000293:Adolescent; D000328:Adult; D000368:Aged; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D008407:Mast Cells / Q000378:metabolism; D008875:Middle Aged; D018317:Nerve Sheath Neoplasms / Q000235:genetics / Q000378:metabolism*; D039842:Neural Cell Adhesion Molecule L1 / Q000235:genetics / Q000378:metabolism*; D009455:Neurofibroma / Q000235:genetics / Q000378:metabolism*; D009456:Neurofibromatosis 1 / Q000235:genetics / Q000378:metabolism; D012189:Retrospective Studies; D055815:Young Adult
D039842:Neural Cell Adhesion Molecule L1