Antioxidant, anti-inflammatory, and anti-senescence activities of a phlorotannin-rich natural extract from brown seaweed Ascophyllum nodosum.
Aging at the cellular level is characterized by oxidative stress, inflammation, and cell senescence. An extract of the brown seaweed Ascophyllum nodosum rich in phlorotannins has been studied for its inhibitory activity against oxidative stress, inflammation, and senescence. A. nodosum extract at 0.2 % prevented tBHP-induced reactive oxygen species production (evaluated using the H2DCF-DA test in cytofluorometry) in epithelial cells and LPS-induced TNF-α and IL-6 release (evaluated using ELISA technique) in macrophages. A. nodosum extract also increased nuclear SIRT1 activity in epithelial cells. Altogether, these beneficial cellular effects of phlorotannin-rich A. nodosum extract could be used in topical therapeutic formulations against aging.
Yslab, 2 rue Félix Le Dantec, 29000 Quimper, France. melody.dutot@yslab.fr
Mélody
M
Dutot
Roxane
R
Fagon
Marc
M
Hemon
Patrice
P
Rat
D000375:Aging / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D044646:Ascophyllum / Q000737:chemistry*; D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D004146:Dioxanes / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012624:Seaweed / Q000737:chemistry*; D013634:Tannins / Q000302:isolation & purification / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016207:Cytokines; D004146:Dioxanes; D017382:Reactive Oxygen Species; D013634:Tannins