Biodiesel synthesis by a one-step method in a genetically engineered Escherichia coli using rice straw hydrolysate and restaurant oil wastes as raw materials.
OBJECTIVE
To reduce the cost of biodiesel, the utilization of rice straw hydrolysate and restaurant oil wastes as the substrates for biodiesel production in a recombinant strain by a one-step method was investigated.


RESULTS
A recombinant Escherichia coli pET28a (+)-PAW, which encodes enzymes for the ethanol pathway and acyltransferase, was constructed. Fermentation results in Luria-Bertani medium revealed that xylose was favourable for fatty acid ethyl esters (FAEEs) production (2.79 g l(-1) ) by E. coli pET28a (+)-PAW. When rice straw hydrolysate and restaurant oil wastes were utilized as the monosaccharide substituent and the fatty acids substituent, 0.27 g l(-1) FAEEs and 0.249 g l(-1) FAEEs were obtained, respectively. 1.27 g l(-1) FAEEs was obtained in the restaurant oil wastes medium supplemented by 2% sodium oleate.


CONCLUSIONS
The fermentation results indicated that the strain was effective in biodiesel production. The rice straw hydrolysate and restaurant oil wastes could be utilized by E. coli as the substrates for FAEE production.


CONCLUSIONS
This novel exploration might pave the way for biodiesel production from waste materials by genetically engineered microorganism.
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, China.
B
B
Wang
H
H
Lin
J
J
Zhan
Y
Y
Yang
Q
Q
Zhou
Y
Y
Zhao
D056804:Biofuels / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry; D005285:Fermentation; D005818:Genetic Engineering; D005947:Glucose / Q000737:chemistry; D009821:Oils / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry*; D012147:Restaurants; D014866:Waste Products; D014994:Xylose / Q000737:chemistry
D056804:Biofuels; D003470:Culture Media; D005227:Fatty Acids; D009821:Oils; D014866:Waste Products; D014994:Xylose; D005947:Glucose