Are titin properties reflected in single myofibrils?
Titin is a structural protein in muscle that spans the half sarcomere from Z-band to M-line. Although there are selected studies on titin's mechanical properties from tests on isolated molecules or titin fragments, little is known about its behavior within the structural confines of a sarcomere. Here, we tested the hypothesis that titin properties might be reflected well in single myofibrils. Single myofibrils from rabbit psoas were prepared for measurement of passive stretch-shortening cycles at lengths where passive titin forces occur. Three repeat stretch-shortening cycles with magnitudes between 1.0 and 3.0μm/sarcomere were performed at a speed of 0.1μm/s·sarcomere and repeated after a ten minute rest at zero force. These tests were performed in a relaxation solution (passive) and an activation solution (active) where cross-bridge attachment was inhibited with 2,3 butanedionemonoxime. Myofibrils behaved viscoelastically producing an increased efficiency with repeat stretch-shortening cycles, but a decreased efficiency with increasing stretch magnitudes. Furthermore, we observed a first distinct inflection point in the force-elongation curve at an average sarcomere length of 3.5μm that was associated with an average force of 68±5nN/mm. This inflection point was thought to reflect the onset of Ig domain unfolding and was missing after a ten minute rest at zero force, suggesting a lack of spontaneous Ig domain refolding. These passive myofibrillar properties observed here are consistent with those observed in isolated titin molecules, suggesting that the mechanics of titin are well preserved in isolated myofibrils, and thus, can be studied readily in myofibrils, rather than in the extremely difficult and labile single titin preparations.
University of Calgary, Faculty of Kinesiology, 2500 University Drive, N.W., Calgary, AB, Canada T2N 1N4.
Jens A
JA
Herzog
Tim R
TR
Leonard
Azim
A
Jinha
Walter
W
Herzog
D000818:Animals; D064211:Connectin; D055119:Elastic Modulus / Q000502:physiology; D008565:Membrane Proteins / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D009124:Muscle Proteins / Q000737:chemistry* / Q000502:physiology* / Q000648:ultrastructure; D009210:Myofibrils / Q000737:chemistry* / Q000502:physiology* / Q000648:ultrastructure; D011494:Protein Kinases / Q000737:chemistry* / Q000502:physiology* / Q000648:ultrastructure; D011817:Rabbits; D013718:Tensile Strength / Q000502:physiology
D064211:Connectin; D008565:Membrane Proteins; D009124:Muscle Proteins; D011494:Protein Kinases