Expression of ferritin-like protein in Listeria monocytogenes after cold and freezing stress.
The cold shock protein family consists of the transfer of the foodborne pathogen Listeria monocytogenes from 37 to 4 and -20 °C and was characterized by the sharp induction of a low molecular mass protein. This major cold shock protein ferritin-like protein (Flp) has an important role in regulation of various microbial physiological processes. Flp have a molecular mass of about 18 kDa, as observed on SDS-PAGE. The purification procedure including ammonium sulfate fractionation was used. Monospecific polyclonal antibodies raised in rabbits against the purified new Flp immunostained a single 18-kDa Flp band in extracts from different cytoplasmic proteins blotted onto nitrocellulose. A 411-bp cDNA fragment that corresponds to an internal region of an flp gene was obtained by RT-PCR. Our result indicated a surexpression of major cold shock protein and an important increase in flp mRNA amount after a downshift temperature especially at -20 °C.
Laboratoire d'Analyses, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Faculté de Pharmacie, rue Avicenne 5000, Monastir, Tunisia. miladi_h@yahoo.fr
Hanene
H
Miladi
Abdelaziz
A
Soukri
Amina
A
Bakhrouf
Emna
E
Ammar
D000645:Ammonium Sulfate / Q000378:metabolism; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D015153:Blotting, Western; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D003080:Cold Temperature; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D005615:Freezing; D020869:Gene Expression Profiling; D008089:Listeria monocytogenes / Q000528:radiation effects*; D008970:Molecular Weight; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; C418864:ferritin-like protein, Listeria; D005293:Ferritins; D000645:Ammonium Sulfate