Discrete bimodal probes for thrombus imaging.
Here we report a generalizable solid/solution-phase strategy for the synthesis of discrete bimodal fibrin-targeted imaging probes. A fibrin-specific peptide was conjugated with two distinct imaging reporters at the C- and N-termini. In vitro studies demonstrated retention of fibrin affinity and specificity. Imaging studies showed that these probes could detect fibrin over a wide range of probe concentrations by optical, magnetic resonance, and positron emission tomography imaging.
A. A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Suite 2301, Charlestown, Massachusetts 02129, USA.
Ritika
R
Uppal
Kate L
KL
Ciesienski
Daniel B
DB
Chonde
Galen S
GS
Loving
Peter
P
Caravan
D005337:Fibrin / Q000031:analogs & derivatives; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005682:Gadolinium; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D010455:Peptides; D049268:Positron-Emission Tomography / Q000379:methods; D013927:Thrombosis / Q000175:diagnosis*
C503783:EP-2104R; D010455:Peptides; D005337:Fibrin; D005682:Gadolinium; D016650:Fluorescein-5-isothiocyanate