Enrichment of colorectal cancer stem cells through epithelial-mesenchymal             transition via CDH1 knockdown.
Cancer stem cells (CSCs) are considered to be the origin of cancer relapse             and metastasis. The better understanding of CSCs, including CSCs in human colorectal             cancer (CRC), may facilitate prevention and treatment. This study aimed to establish             a CSC enrichment model via the induction of epithelial-mesenchymal transition             (EMT) in CRC cells. We established an EMT model using the SW480 CRC cell line             by CDH1 knockdown using shRNA interference. CD24+CD44+ cells were enriched in             the CDH1 knockdown cells. The cells exhibited mesenchymal morphology and expressed             high levels of EMT-related proteins, which confirmed that these cells had undergone             EMT. Our results further showed that the proliferation rate of the transfected             cells was reduced, whereas their colony-forming capacity and tumorigenesis in vivo             was significantly enhanced compared to the control cells. In conclusion, these             cells were highly enriched CSCs (compared to normal CSCs) and may be used as a             stable model for cancer research and anticancer drug screening.
Cancer Institute, Key Laboratory for Cancer Prevention and Intervention, China National Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, PR China.
Jun
J
Ye
Dang
D
Wu
Jinwen
J
Shen
Pin
P
Wu
Chao
C
Ni
Jing
J
Chen
Jing
J
Zhao
Tao
T
Zhang
Xiaolei
X
Wang
Jian
J
Huang
D000818:Animals; D015703:Antigens, CD; D051927:CD24 Antigen / Q000378:metabolism; D015820:Cadherins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014183:Transplantation, Heterologous
D015703:Antigens, CD; D051927:CD24 Antigen; C513384:CDH1 protein, human; D015820:Cadherins; D018960:Hyaluronan Receptors; D034741:RNA, Small Interfering