Determination of effective charge of small ions, polyelectrolytes and nanoparticles by capillary electrophoresis.
In this paper, a systematic and comparative study related to the effective charge determination of three kinds of solutes (small ions, polyelectrolytes and nanoparticles) was performed. Four approaches were compared regarding their conditions of validity and their advantages/disadvantages. Three of them allow the effective charge determination from the electrophoretic mobility and the hydrodynamic radius of the solutes using electrophoretic mobility modelings based on Nernst-Einstein (NE), O'Brien-White-Ohshima (OWO) and Yoon and Kim (YK) equations. Electrophoretic mobility and hydrodynamic radius were determined by capillary electrophoresis and Taylor dispersion analysis, respectively, using the same instrumentation in similar conditions, on a large set of samples. A fourth experimental approach based on the sensitivity of detection in indirect UV detection mode (IUV) was compared to the previously mentioned methods. OWO and YK modelings are well adapted for the effective charge determination of small ions and nanoparticles, while IUV is the only method adapted for polyelectrolytes.
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, Université de Montpellier 1-2, Place Eugène Bataillon CC 1706, 34095 Montpellier Cedex 5, France.
Amal
A
Ibrahim
Hiroyuki
H
Ohshima
Stuart A
SA
Allison
Hervé
H
Cottet
D004573:Electrolytes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D057446:Hydrodynamics; D007477:Ions / Q000737:chemistry; D008956:Models, Chemical; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D004573:Electrolytes; D007477:Ions; D011108:Polymers