In vitro evaluation of the suppressor potential of conditioned medium from benign myoepithelial cells from pleomorphic adenoma in malignant cell invasion.
Tumoral invasion process is the result of a complex interaction between the tumor cells and microenvironment which plays an important role in modulating the growth and invasion of the cancer. The myoepithelial cells, present in glandular organs such as the breast and salivary glands, seem to exert paracrine effects on the glandular epithelium, acting as natural tumor suppressors. To verify the influence of the benign myoepithelial cells in the invasion of malignant cells, simulating an in situ carcinoma ex pleomorphic adenoma, we have cultured three different high-potential invasive malignant tumors (breast ductal adenocarcinoma, melanoma and oral squamous cell carcinoma) in conditioned medium of myoepithelial cells from salivary gland pleomorphic adenomas using transwell chambers with 8-μm pores membrane coated with matrigel. After 96 h, quantitative analyses of the results were performed by calculating the invasion index (number of cells that invaded in relation to the total number of cells). The results showed that there was a reduction of the invasion index mean for the three different malignant tumors. This study supports a tumoral suppressor function of the myoepithelial cells from pleomorphic adenoma in in vitro invasion process.
São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil. alessandradutrasilva@hotmail.com
Alessandra Dutra
AD
da Silva
Carolina Amália Barcellos
CA
Silva
Victor Angelo Martins
VA
Montalli
Elizabeth Ferreira
EF
Martinez
Vera Cavalcanti
VC
de Araújo
Cristiane
C
Furuse
D000199:Actins / Q000032:analysis; D008949:Adenoma, Pleomorphic / Q000473:pathology*; D001672:Biocompatible Materials / Q000737:chemistry; D001943:Breast Neoplasms / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000032:analysis; D002278:Carcinoma in Situ / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D003094:Collagen / Q000737:chemistry; D017077:Culture Media, Conditioned; D015194:Diffusion Chambers, Culture; D004338:Drug Combinations; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D007797:Laminin / Q000737:chemistry; D008545:Melanoma / Q000473:pathology; D008567:Membranes, Artificial; D008840:Microfilament Proteins / Q000032:analysis; D008851:Micropore Filters; D009062:Mouth Neoplasms / Q000473:pathology; D009124:Muscle Proteins / Q000032:analysis; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000473:pathology*; D011509:Proteoglycans / Q000737:chemistry; D059016:Tumor Microenvironment; D014746:Vimentin / Q000032:analysis; D000096985:Calponins
D000199:Actins; D001672:Biocompatible Materials; D002135:Calcium-Binding Proteins; D003094:Collagen; D017077:Culture Media, Conditioned; D004338:Drug Combinations; D007797:Laminin; D008567:Membranes, Artificial; D008840:Microfilament Proteins; D009124:Muscle Proteins; D011509:Proteoglycans; D014746:Vimentin; D000096985:Calponins; C059485:matrigel