Synthesis of cationic poly(4-vinylpyridine)-functionalized colloidal particles by emulsion polymerization with reactive block copolymer for protein adsorption.
We report here a novel protein sequestration method using polymeric colloidal particles prepared by emulsion polymerization with reactive block copolymers. Specifically, poly(4-vinyl-N-ethylpyridine bromide)-block-polymethylacrylate block copolymers were synthesized from reversible addition-fragmentation chain transfer polymerization and used as emulsifiers for emulsion polymerization of poly(4-vinyl-N-ethylpyridine bromide)-functionalized polymeric colloidal particles. The particles have high and stable zeta potentials when dispersed in solution, regardless of pH variations. As a result, the polymeric colloids demonstrate a high affinity for oppositely charged proteins, even though the isoelectric points of proteins may vary greatly. We show here that BSA can be sequestered highly efficiently with a maximum binding capacity (~900 mg/g). The adsorbed protein is easily released, and the polymeric colloids are regenerated after washing with a buffer solution of high ionic strength. These properties may prompt this type of novel macromolecule-functionalized colloids to be utilized for effective protein adsorption and separation.
Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China. cjx724@163.com
Jinxin
J
Cao
Xiaodi
X
Pan
Wei
W
Huang
Yingjie
Y
Wang
Daoben
D
Hua
Xiulin
X
Zhu
Hongjun
H
Liang
D000179:Acrylates / Q000737:chemistry*; D000327:Adsorption; D002412:Cations; D003102:Colloids; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D009994:Osmolar Concentration; D011145:Polyvinyls / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions
D000179:Acrylates; D002412:Cations; D003102:Colloids; D011145:Polyvinyls; D012996:Solutions; C019535:poly(4-vinylpyridine); D012710:Serum Albumin, Bovine