Characterization of MLS(b)  resistance among Staphylococcus aureus and Staphylococcus epidermidis isolates carrying different SCCmec types.
This work characterizes MLS(b)  resistance in 39 methicillin-resistant Staphylococcus aureus (MRSA) and 32 Staphylococcus epidermidis (MRSE) isolates. Of 21 erm(A) gene encoding MRSA isolates, 71.4% carried SCCmecIII, whereas of 12 isolates carrying the erm(C) gene, 83.3% carried SCCmecIV. Among the 25 MRSE isolates positive for the erm(C) gene, 80% had SCCmecIV or nontypeable cassettes. Isolates carrying these genes had MIC(90) ≥ 256 μg/mL to erythromycin and clindamycin. The msr(A) gene was associated with a low MIC(90)  to these drugs. The erm(A) gene was associated with SCCmecIII in MRSA isolates, whereas the erm(C) gene was associated with SCCmecIV in both MRSA and MRSE isolates.
Department of Medical Microbiology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Cristiane R S
CR
Teodoro
Cláudio S
CS
Mattos
Fernanda S
FS
Cavalcante
Eliezer M
EM
Pereira
Kátia R N
KR
dos Santos
D001426:Bacterial Proteins / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D002876:Chromosomes, Bacterial / Q000235:genetics; D002981:Clindamycin / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D004917:Erythromycin / Q000494:pharmacology*; D005798:Genes, Bacterial; D006801:Humans; D007231:Infant, Newborn; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D008780:Methyltransferases / Q000235:genetics; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins; D010641:Phenotype; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D025381:Streptogramin B / Q000494:pharmacology*
D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; D025381:Streptogramin B; D002981:Clindamycin; D004917:Erythromycin; D008780:Methyltransferases; C431709:ErmA protein, Bacteria; C035181:rRNA (adenosine-O-2'-)methyltransferase