[Protective effect of exogenous nucleotides on the developmental toxicity of alcohol].
OBJECTIVE
To investigate the preventive effect of exogenous nucleotide (EN) on the developmental toxicity of alcohol.


METHODS
C57BL/6J pregnant  mice were divided into 6 groups randomly: the control group, the alcohol group, the low (0.01%EN), middle (0.04%EN), high (0.16%EN) and higher (0.64% EN) intervention groups. From the 6th gestational days (GD) to the 15th GD, pregnant animals except those in the control group were administrated with 5 g  (per kg body weight) alcohol intragastrically. Normal mouse forage was provided to the animals in the control group and alcohol group, while forage  containing different quantities of EN was provided to the animals in the intervention groups. On GD 18, all the dams were killed, their blood samples were  collected for further analysis, and fetal developmental indexes were observed.


RESULTS
Compared with the animals in the alcohol group, offspring's  body weight and placenta weight of EN intervention groups improved a lot; superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity  increased significantly (P<0.05), malondialdehyde (MDA) levels decreased significantly (P<0.01); the live birth rate of animals in the interventions groups  has increased significantly and the absorbed embryo rate of them has decreased significantly (P<0.01); the forepaw phalanges, hindpaw phalanges and  caudal vertebrae ossification point increased significantly (P<0.01); the anomalies of both occipital and sternum have decreased significantly in 0.04%EN  group (P<0.05).


CONCLUSIONS
EN has a significant protective effect on the developmental toxicity of alcohol.
Department of Nutrition and Food Hygiene, Peking University School of Public Health, Beijing, China.
Wen-hong
WH
Dong
Jie
J
Zhao
Jia-xi
JX
Zhang
Lin-lin
LL
Xu
Ya-jun
YJ
Xu
D000818:Animals; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000517:prevention & control*; D008297:Male; D018811:Maternal Exposure; D051379:Mice; D008810:Mice, Inbred C57BL; D009711:Nucleotides / Q000494:pharmacology / Q000627:therapeutic use*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000517:prevention & control*
D009711:Nucleotides; D000431:Ethanol