Biodistribution properties of cleistanthin A and cleistanthin B using magnetic resonance imaging in a normal and tumoric animal model.
OBJECTIVE
To determine the biodistribution properties of cleistanthin A and cleistanthin B in rodents using magnetic resonance imaging (MRI).


METHODS
Cleistanthins A and B, constituents of Cleistanthus collinus Roxb., were labelled with gadolinium (Gd(3+)) directly and injected into normal and tumoric nude mice. The tissue signal intensity was measured using MRI to perform a noninvasive kinetic assay. Wistar rats were used for determination of the grayscale intensity to observe the distribution patterns of of cleistanthins A and B.


RESULTS
Cleistanthin A is kinetically more attractive to the gastrointestinal tract than is cleistanthin B, which gets accumulated in muscular tissues of mice in greater concentrations compared with cleistanthin A. Cleistanthin B but not cleistanthin A showed tumoric affinity and exhibited a tumor kinetic attraction in tumoric mice. In rats, cleistanthin A showed greater grayscale intensities in the brain, liver, and skeletal muscles in immediate post contrast MRI images, whereas the gadolinium tagged cleistanthin B showed higher grayscale intensities in the cardiac muscle and skeletal muscles in delayed post contrast MRI images.


CONCLUSIONS
Cleistanthin A is more pharmacokinetically attractive to the gastrointestinal tract than cleistanthin B.
Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
Subramani
S
Parasuraman
Ramasamy
R
Raveendran
Mehdi Shafiee
MS
Ardestani
Ramesh
R
Ananthakrishnan
Ali
A
Jabbari-Arabzadeh
Mohammad Shafiee
MS
Alavidjeh
Mohammad Reza
MR
Aghasadeghi
Sundararajan
S
Elangovan
Halanaik
H
Dhanapathi