Elastic volume reconstruction from series of ultra-thin microscopy sections.
Anatomy of large biological specimens is often reconstructed from serially sectioned volumes imaged by high-resolution microscopy. We developed a method to reassemble a continuous volume from such large section series that explicitly minimizes artificial deformation by applying a global elastic constraint. We demonstrate our method on a series of transmission electron microscopy sections covering the entire 558-cell Caenorhabditis elegans embryo and a segment of the Drosophila melanogaster larval ventral nerve cord.
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Stephan
S
Saalfeld
Richard
R
Fetter
Albert
A
Cardona
Pavel
P
Tomancak
D000818:Animals; D017173:Caenorhabditis elegans / Q000648:ultrastructure; D004331:Drosophila melanogaster / Q000648:ultrastructure; D004548:Elasticity; D004625:Embryo, Nonmammalian / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D007814:Larva / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission / Q000295:instrumentation / Q000379:methods*; D008867:Microtomy / Q000295:instrumentation / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*