[Cloning and expression in follicle anagen of ILK gene in sheep].
Integrin linked kinase (ILK) is a scaffold protein, which plays important roles in hair follicle development. The cDNA sequence of novel ILK gene in sheep was cloned by PCR method and analyzed by bioinformatics. Tissue expression profiling in eight tissues and temporal profiling at different wool follicle anagen stages in skin was analyzed. The results showed that the whole open reading frame (ORF) of ILK gene was 1 359 bp in length, which encoded 452 amino acids. Bioinformatic analysis indicated that the secondary structure of ILK gene was mainly made up of three ankyrin repeats and a kinase domain, and there were multiple phosphorylation and Protein Kinase C sites in this gene. The RT-PCR result confirmed that ILK mRNA was expressed in heart, liver, spleen, lung, skeletal muscle, skin, and small intestine, and the expression level was much higher in skin, spleen, and liver than others. The q-PCR analysis demonstrated that the ex-pression level of ILK was significantly increased from March to May (early follicle anagen initiation) in both sheep breeds, Chinese Merino and Kazakh sheep, and there were certain differences from June to October between the two breeds. The above results indicated that ILK gene may play key roles in regulating secondary follicle growth.
College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.
Jian-Bo
JB
Yang
Shang-Quan
SQ
Gan
Yong-Lin
YL
Yang
Hong-Lin
HL
Zhang
Tian-Zeng
TZ
Song
Jing
J
Feng
Jing-Quan
JQ
Yang
Lei
L
Gao
Guo-Qing
GQ
Shi
Min
M
Shen
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D019295:Computational Biology / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D018859:Hair Follicle / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D012756:Sheep / Q000235:genetics* / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D000099200:Scaffold Protein ILK
D018076:DNA, Complementary; D017346:Protein Serine-Threonine Kinases; D000099200:Scaffold Protein ILK