Moloney murine leukemia virus integrase and reverse transcriptase interact with PML proteins.
Pull-down assay and co-immunoprecipitation of cell extracts in which the integrase or reverse transcriptase of Moloney murine leukemia virus was transiently expressed showed that both enzymes interacted with PML proteins. In infected cells, interaction between the integrase and PML was also observed. Transient expression of PIASy and SUMO proteins facilitated SUMOylation of the integrase but had no apparent effects on the interaction with PML. A FLAG-tagged integrase co-localized with PML protein possibly in the PML body. Knockdown of PML by small interfering RNA resulted in reduced viral cDNA levels and integration efficiency. This suggested that PML proteins activated reverse transcription.
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, 464-8603, Japan.
Yuuki
Y
Okino
Yujin
Y
Inayoshi
Yusuke
Y
Kojima
Shunsuke
S
Kidani
Hidenori
H
Kaneoka
Akiko
A
Honkawa
Hiroshi
H
Higuchi
Ken-ichi
K
Nishijima
Katsuhide
K
Miyake
Shinji
S
Iijima
D000818:Animals; D022003:Cell Nucleus Structures / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008979:Moloney murine leukemia virus / Q000201:enzymology*; D041681:NIH 3T3 Cells / Q000821:virology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D000071156:Promyelocytic Leukemia Protein; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
D009687:Nuclear Proteins; C513293:Pml protein, mouse; D000071156:Promyelocytic Leukemia Protein; D025842:SUMO-1 Protein; D025841:Small Ubiquitin-Related Modifier Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human; D019426:Integrases; D012194:RNA-Directed DNA Polymerase