Characterization of the Salmonella bacteriophage vB_SenS-Ent1.
The bacteriophage vB_SenS-Ent1 (Ent1) is a member of the family Siphoviridae of tailed bacteriophages and infects a broad range of serovars of the enteric pathogen Salmonella enterica. The virion particle is composed of an icosahedral head 64 nm in diameter and a flexible, non-contractile tail of 116 × 8.5 nm possessing terminal fibres. The adsorption rate constant at 37 °C is 6.73 × 10(-9) ml min(-1). Latent and eclipse periods are 25 and 20 min, respectively, and the burst size is 35 progeny particles per cell after 35 min at 37 °C. Sequencing revealed a circularly permuted, 42 391 bp dsDNA genome containing 58 ORFs organized into four major transcriptional units. Comparisons with the genome sequences of other bacteriophages revealed a high level of nucleotide sequence identity and shared orthologous proteins with the Salmonella phages SETP3, SE2 and KS7 (SS3e) and the Escherichia phages K1G, K1H, K1ind1 and K1ind3.
Centre for Research in Biosciences, Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK.
Dann
D
Turner
Centre for Research in Biosciences, Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK.
Mohammed
M
Hezwani
Centre for Research in Biosciences, Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK.
Shona
S
Nelson
Centre for Research in Biosciences, Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK.
Vyv
V
Salisbury
Centre for Research in Biosciences, Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK.
Darren
D
Reynolds
D001483:Base Sequence; D016679:Genome, Viral; D058507:Host Specificity; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012483:Salmonella Phages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D019779:Salmonella enterica / Q000821:virology*; D012722:Sewage / Q000382:microbiology*; D017906:Siphoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
D012722:Sewage; D014764:Viral Proteins