Structure of the lipopolysaccharide isolated from the novel species Uruburuella suis.
Uruburuella suis is a novel species isolated from lungs and heart of pigs with pneumonia and pericarditis. Phenotypic and phylogenetic evidences showed that it represented a hitherto unknown subline within the family Neisseriaceae. In the present work we defined the whole structure of the LPS isolated from Uruburuella suis. The structural determination, which was achieved by chemical, spectroscopic and spectrometric approaches, indicates a novel rough type lipopolysaccharide rich in negatively charged groups in the lipid A-inner core region. The elucidation of the structural features of the LPS from Uruburuella suis is a first step toward the comprehension of the characteristics of the cell envelope in such new and interesting microorganisms.
Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy. silipo@unina.it
Alba
A
Silipo
Luisa
L
Sturiale
Cristina
C
De Castro
Rosa
R
Lanzetta
Michelangelo
M
Parrilli
Domenico
D
Garozzo
Antonio
A
Molinaro
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D011232:Chemical Precipitation; D006834:Hydrazines / Q000737:chemistry; D008050:Lipid A / Q000737:chemistry* / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006834:Hydrazines; D008050:Lipid A; D008070:Lipopolysaccharides; C029424:hydrazine