[CSF RT-PCR standardization in pediatric patients with enteroviral central nervous system infection].
OBJECTIVE
We standardized the RT-PCR panviral CSF and determined its applicability in detecting acute enterovirus infection in the central nervous system in children under 15 years.


METHODS
RT-PCR was performed directly in CSF samples of 10 pediatric patients with suspected CNS infection and 9, with different conditions of the central nervous system.


RESULTS
80% (8/10) of RT-PCR samples were positive for enterovirus in patients with suspected CNS infection and no sample was positive in patients with different ailments.


CONCLUSIONS
Since enteroviruses are among the main etiologies of pediatric encephalitis, RT-PCR could be particularly useful for rapid detection in CSF.
Unidad de Investigación en Inmunología e Infectología, Hospital de Infectología Dr. Federico Méndez, Centro Médico Nacional La Raza, IMSS, México, DF. gelorriaga@webtelmex.net.mx
Guadalupe
G
García-Elorriaga
Amalia
A
Esparza-García
Claudia
C
Méndez-Rojas
Guillermo
G
del Rey-Pineda
César
C
González-Bonilla
D000208:Acute Disease; D002555:Cerebrospinal Fluid / Q000737:chemistry / Q000821:virology; D002648:Child; D002653:Child Behavior Disorders / Q000134:cerebrospinal fluid / Q000209:etiology; D002675:Child, Preschool; D003244:Consciousness Disorders / Q000134:cerebrospinal fluid / Q000209:etiology; D018792:Encephalitis, Viral / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000821:virology; D004769:Enterovirus Infections / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000821:virology; D005260:Female; D005335:Fever of Unknown Origin / Q000134:cerebrospinal fluid / Q000209:etiology; D006801:Humans; D007223:Infant; D008297:Male; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000821:virology; D010865:Pilot Projects; D012367:RNA, Viral / Q000134:cerebrospinal fluid; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012640:Seizures / Q000134:cerebrospinal fluid / Q000209:etiology
D012367:RNA, Viral