The importance of genetic variants in TNFα for periodontal disease in a cohort of coronary patients.
OBJECTIVE
The aim of this analysis was to evaluate the importance of genetic variants of TNFα for the severity of periodontal disease and periodontal risk factors with respect to periodontal risk factors in a cohort of coronary patients.


METHODS
A total of 942 consecutive patients with angiographic proven coronary heart disease were prospectively included in the study entitled "Periodontitis and Its Microbiological Agents as Prognostic Factors in Patients With Coronary Heart Disease" (ClinicalTrials.gov identifier:NCT01045070).


METHODS
After including of patients, an extensive periodontal examination also involving PCR-sampling for 11 periodontal bacteria was performed. In this subanalysis, single nucleotide polymorphisms (SNPs) c.-308G>A, c.-238G>A and haplotypes for TNFα were analysed by CTS-PCR-SSP Tray kit (Heidelberg, Germany).


RESULTS
The AG+AA genotype of SNP c.-238G>A of TNFα gene was associated with the amount of clinical attachment loss in patients with coronary heart disease in multivariate regression analysis. Moreover, Prevotella intermedia occurred more frequently in carriers who were positive for the AG+AA genotype and A-allele of SNP c.-308G>A in bivariate and multivariate analyses. Furthermore, only in bivariate analyses significant associations of genetic variants of TNFα with intensified bleeding on probing and with higher plasma level of interleukin 6 could be shown.


CONCLUSIONS
Genetic variants of TNFα gene, namely c.-308G>A and c.-238G>A, are associated with periodontal conditions in patients with coronary heart disease.
Department of Operative Dentistry and Periodontology, Martin-Luther University Halle-Wittenberg, Halle, Germany. susanne.schulz@medizin.uni-halle.de
Susanne
S
Schulz
Axel
A
Schlitt
Andrea
A
Lutze
Sandra
S
Lischewski
Tim
T
Seifert
Tanya
T
Dudakliewa
Robert
R
Gawe
Karl
K
Werdan
Britt
B
Hofmann
Christiane
C
Gläser
Hans-Günter
HG
Schaller
Stefan
S
Reichert
D000225:Adenine; D000368:Aged; D000483:Alleles; D001419:Bacteria / Q000145:classification / Q000235:genetics; D015331:Cohort Studies; D003327:Coronary Disease / Q000150:complications*; D003774:Dental Plaque Index; D048909:Diabetes Complications; D005051:Eubacterium / Q000302:isolation & purification; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D005884:Gingival Hemorrhage / Q000276:immunology / Q000382:microbiology; D006147:Guanine; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D017622:Periodontal Attachment Loss / Q000276:immunology / Q000382:microbiology; D010514:Periodontal Pocket / Q000276:immunology / Q000382:microbiology; D010518:Periodontitis / Q000276:immunology* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D018713:Prevotella intermedia / Q000302:isolation & purification; D011446:Prospective Studies; D012307:Risk Factors; D012737:Sex Factors; D012907:Smoking; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D006147:Guanine; D000225:Adenine