[Comparison of phenotype and functions of dendritic cells in culture medium supplemented with different serum or plasma].
OBJECTIVE
To  find a safe and effective method of culturing dendritic cells (DCs) for clinical application  by comparing the phenotype and functions of DCs  in  different culture mediums which are supplemented with 100 mL/L fetal bovine serum (FBS),  20 mL/L human AB serum and 10 mL/L autologous plasma.


METHODS
Peripheral blood mononuclear cells (PBMCs) were induced to DCs in 100 mL/L FBS,  20 mL/L human AB serum,  and 10 mL/L autologous plasma,  respectively. On day 8,  the purity,  phenotype and antigen uptake capability of DCs were examined by flow cytometry. The mixed lymphocyte reaction (MLR) was detected by MTT assay.


RESULTS
Focused on DC aggregates,  DC yield,  DC purity,  antigen uptake capability and ability of stimulating lymphocyte proliferation,  the experiments showed  that those of  100 mL/L FBS culture group were better than those of  20 mL/L human AB serum and 10 mL/L autologous plasma culture groups.  There was no significant difference among these three culture groups (P>0.05) except DC yield (P<0.05).


CONCLUSIONS
Autologous plasma is the best choice for DC cultivation among these three culture ways. It is a safe and efficient way for culturing DCs for  clinical  purpose.
Institute of Human Reproduction and Stem Cell Engineering, Central South University, Changsha, China. yihuijun888@gmail.com
Hui-jun
HJ
Yi
Guang-xiu
GX
Lu
D002478:Cells, Cultured; D003470:Culture Media; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D010641:Phenotype; D010949:Plasma / Q000378:metabolism*; D044967:Serum / Q000378:metabolism*
D003470:Culture Media