Characterization and cloning of GNA-like lectin from the mushroom Marasmius oreades.
A new mannose-recognizing lectin (MOL) was purified on an asialofetuin-column from fruiting bodies of Marasmius oreades grown in Japan. The lectin (MOA) from the fruiting bodies of the same fungi is well known to be a ribosome-inactivating type lectin that recognizes blood-group B sugar. However, in our preliminary investigation, MOA was not found in Japanese fruiting bodies of M. oreades, and instead, MOL was isolated. Gel filtration showed MOL is a homodimer noncovalently associated with two subunits of 13 kDa. The N-terminal sequence of MOL was blocked. The sequence of MOL was determined by cloning from cDNA and by protein sequencing of enzyme-digested peptides. The sequence shows mannose-binding motifs of bulb-type mannose-binding lectins from plants, and similarity to the sequences. Analyses of sugar-binding specificity by hemagglutination inhibition revealed the preference of MOL toward mannose and thyroglobulin, but asialofetuin was the strongest inhibitor of glycoproteins tested. Furthermore, glycan-array analysis showed that the specificity pattern of MOL was different from those of typical mannose-specific lectins. MOL preferred complex-type N-glycans rather than high-mannose N-glycans.
Department of Applied Biological Chemistry, The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Michiko
M
Shimokawa
Ayako
A
Fukudome
Ryoko
R
Yamashita
Yuji
Y
Minami
Fumio
F
Yagi
Hiroaki
H
Tateno
Jun
J
Hirabayashi
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001212:Asialoglycoproteins / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D060748:Fetuins / Q000737:chemistry / Q000378:metabolism; D048690:Fruiting Bodies, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007564:Japan; D037601:Mannose-Binding Lectin / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D055433:Marasmius / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D055503:Protein Multimerization; D011817:Rabbits; D013954:Thyroglobulin / Q000737:chemistry / Q000378:metabolism; D014312:Trisaccharides / Q000737:chemistry / Q000378:metabolism
D001212:Asialoglycoproteins; D060748:Fetuins; D005656:Fungal Proteins; D037601:Mannose-Binding Lectin; D014312:Trisaccharides; C017029:asialofetuin; C406478:blood group B trisaccharide; D013954:Thyroglobulin