Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs.
Transcription activator-like effector nucleases (TALENs) are powerful new research tools that enable targeted gene disruption in a wide variety of model organisms. Recent work has shown that TALENs can induce mutations in endogenous zebrafish genes, but to date only four genes have been altered, and larger-scale tests of the success rate, mutation efficiencies and germline transmission rates have not been described. Here, we constructed homodimeric TALENs to 10 different targets in various endogenous zebrafish genes and found that 7 nuclease pairs induced targeted indel mutations with high efficiencies ranging from 2 to 76%. We also tested obligate heterodimeric TALENs and found that these nucleases induce mutations with comparable or higher frequencies and have better toxicity profiles than their homodimeric counterparts. Importantly, mutations induced by both homodimeric and heterodimeric TALENs are passed efficiently through the germline, in some cases reaching 100% transmission. For one target gene sequence, we observed substantially reduced mutagenesis efficiency for a variant site bearing two mismatched nucleotides, raising the possibility that TALENs might be used to perform allele-specific gene disruption. Our results suggest that construction of one to two heterodimeric TALEN pairs for any given gene will, in most cases, enable researchers to rapidly generate knockout zebrafish.
Cardiovascular Research Center, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Charlestown, MA 02129, USA.
Lindsay
L
Cade
Deepak
D
Reyon
Woong Y
WY
Hwang
Shengdar Q
SQ
Tsai
Samir
S
Patel
Cyd
C
Khayter
J Keith
JK
Joung
Jeffry D
JD
Sander
Randall T
RT
Peterson
Jing-Ruey Joanna
JR
Yeh
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000378:metabolism*; D019281:Dimerization; D055786:Gene Knockout Techniques; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D015534:Trans-Activators / Q000737:chemistry; D015027:Zebrafish / Q000235:genetics*
D004268:DNA-Binding Proteins; D015534:Trans-Activators; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific