Fluorochrome-functionalized magnetic nanoparticles for high-sensitivity monitoring of the polymerase chain reaction by magnetic resonance.
Easy to find: magnetic nanoparticles bearing fluorochromes (red) that intercalate with DNA (green) form microaggregates with DNA generated by the polymerase chain reaction (PCR). These aggregates can be detected at low cycle numbers by magnetic resonance (MR).
Center for Translational Nuclear Medicine and Molecular Imaging, Massachusetts General Hospital, Charlestown, 02129, USA.
David
D
Alcantara
Yanyan
Y
Guo
Hushan
H
Yuan
Craig J
CJ
Goergen
Howard H
HH
Chen
Hoonsung
H
Cho
David E
DE
Sosnovik
Lee
L
Josephson
D017209:Apoptosis; D004247:DNA / Q000378:metabolism*; D052203:Ferrosoferric Oxide / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006397:Hematinics / Q000378:metabolism; D007364:Intercalating Agents / Q000494:pharmacology; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D009930:Organic Chemicals / Q000378:metabolism; D016133:Polymerase Chain Reaction
D005456:Fluorescent Dyes; D006397:Hematinics; D007364:Intercalating Agents; D009930:Organic Chemicals; C405903:TO-PRO-1; D004247:DNA; D052203:Ferrosoferric Oxide