Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract.
BACKGROUND
Magnolia grandiflora L. flower is wildly used in Asian as a traditional herbal medication. The purpose of the study was to investigate the antimelanogenic and antioxidant properties of Magnolia grandiflora L. flower extract. In the study, the inhibitory effects of M. grandiflora L. flower extract on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were determined spectrophotometrically. Meanwhile, the antioxidative capacity of the flower extract was also investigated.


RESULTS
Our results revealed that M. grandiflora L. flower extract inhibit mushroom tyrosinase activity (IC(50) = 11.1%; v/v), the flower extract also effectively suppressed intracellular tyrosinase activity (IC(50) = 13.6%; v/v) and decreased the amount of melanin (IC(50) = 25.6%; v/v) in a dose-dependent manner in B16F10 cells. Protein expression level of tyrosinase and tyrosinase-related protein 1 (TRP-1) were also decreased by the flower extract. Additionally, antioxidant capacities such as ABTS(+) free radical scavenging activity, reducing capacity and total phenolic content of the flower extract were increased in a dose-dependent pattern.


CONCLUSIONS
Our results concluded that M. grandiflora L. flower extract decreased the expression of tyrosinase and TRP-1, and then inhibited melanogenesis in B16F10 cells. The flower extract also show antioxidant capacities and depleted cellular reactive oxygen species (ROS). Hence, M. grandiflora L. flower extract could be applied as a type of dermatological whitening agent in skin care products.
Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan.
Huey-Chun
HC
Huang
Wan-Yu
WY
Hsieh
Yu-Lin
YL
Niu
Tsong-Min
TM
Chang
D000363:Agaricales / Q000201:enzymology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D052160:Benzothiazoles; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D035264:Flowers; D006801:Humans; D031566:Magnolia; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
D000975:Antioxidants; D052160:Benzothiazoles; D008543:Melanins; D008562:Membrane Glycoproteins; D010636:Phenols; D010936:Plant Extracts; D013451:Sulfonic Acids; D013844:Thiazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D010088:Oxidoreductases; C489799:TYRP1 protein, human; D014442:Monophenol Monooxygenase