Three-dimensional evaluation of the spinal local neural network revealed by the high-voltage electron microscopy: a double immunohistochemical study.
Three-dimensional (3-D) analysis of anatomical ultrastructures is important in biological research. However, 3-D image analysis on exact serial sets of ultra-thin sections from biological specimens is very difficult to achieve, and limited information can be obtained by 3-D reconstruction from these sections due to the small area that can be reconstructed. On the other hand, the high-penetration power of electrons by an ultra-high accelerating voltage enables thick sections of biological specimens to be examined. High-voltage electron microscopy (HVEM) is particularly useful for 3-D analysis of the central nervous system because considerably thick sections can be observed at the ultrastructure level. Here, we applied HVEM tomography assisted by light microscopy to a study of the 3-D chemical neuroanatomy of the rat lower spinal cord annotated by double-labeling immunohistochemistry. This powerful methodology is useful for studying molecular and/or chemical neuroanatomy at the 3-D ultrastructural level.
Ushimado Marine Institute, Graduate School of Natural Science and Technology, Okayama University, 130-17 Kashino, Ushimado, Setouchi, Okayama 701-4303, Japan.
Takumi
T
Oti
Keita
K
Satoh
Kazuhiro
K
Saito
Kazuyoshi
K
Murata
Mitsuhiro
M
Kawata
Tatsuya
T
Sakamoto
Hirotaka
H
Sakamoto
D000818:Animals; D005260:Female; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009415:Nerve Net / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000648:ultrastructure*