Characterization of porcine SKIP gene in skeletal muscle development: polymorphisms, association analysis, expression and regulation of cell growth in C2C12 cells.
Skeletal muscle and kidney-enriched inositol phosphatase (SKIP) was identified as a 5'-inositol phosphatase that hydrolyzes phosphatidylinositol (3,4,5)-triphosphate (PI(3,4,5)P3) to PI(3,4)P2 and negatively regulates insulin-induced phosphatidylinositol 3-kinase signaling in skeletal muscle. In this study, two new single nucleotide polymorphisms (SNPs) in porcine SKIP introns 1 and 6 were detected. The C1092T locus in intron 1 showed significant associations with some meat traits, whereas the A17G locus in intron 6 showed significant associations with some carcass traits. Expression analysis showed that porcine SKIP is upregulated at d 65 of gestation and Meishan fetuses have higher and prolonged expression of SKIP compared to Large White at d 100 of gestation. Ectopic expression of porcine SKIP decreased insulin-induced cell proliferation and promoted serum starvation-induced cell cycle arrest in G0/G1 phase in C2C12. Our results suggest that SKIP plays a negative regulatory role in skeletal muscle development partly by preventing cell proliferation.
Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Qi
Q
Xiong
Jin
J
Chai
Changyan
C
Deng
Siwen
S
Jiang
Yang
Y
Liu
Tao
T
Huang
Xiaojun
X
Suo
Nian
N
Zhang
Xiaofeng
X
Li
Qianping
Q
Yang
Mingxin
M
Chen
Rong
R
Zheng
D000818:Animals; D017849:Animals, Inbred Strains; D001823:Body Composition; D002460:Cell Line; D049109:Cell Proliferation; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D056726:Genetic Association Studies / Q000662:veterinary; D007438:Introns; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008460:Meat / Q000032:analysis; D051379:Mice; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D034622:RNA Interference; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D013045:Species Specificity; D034421:Sus scrofa / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism
D007527:Isoenzymes; D011994:Recombinant Proteins; D010744:Phosphoric Monoester Hydrolases