Flii neutralizing antibodies improve wound healing in porcine preclinical studies.
Wound healing is an important area of widely unmet medical need, with millions of procedures carried out worldwide which could potentially benefit from a product to improve the wound repair process. Our studies investigating the actin-remodeling protein Flightless I (Flii) show it to be an important regulator of wound healing. Flii-deficient mice have enhanced wound healing in comparison to Flii overexpressing mice which have impaired wound healing. For the first time, we show that a Flightless I neutralizing monoclonal antibody (FnAb) therapy is effective in a large animal model of wound repair. Porcine 5 cm incisional and 6.25 cm(2) excisional wounds were treated with FnAb at the time of wounding and for two subsequent days. The wounds were dressed in Tegaderm dressings and left to heal by secondary intention for 7 and 35 days, respectively. At the relevant end points, the wounds were excised and processed for histological analysis. Parameters of wound area, collagen deposition, and scar appearance were analyzed. The results show that treatment with FnAb accelerates reepithelialization and improves the macroscopic appearance of early scars. FnAbs have the potential to enhance wound repair and reduce scar formation.
Women's and Children's Health Research Institute, North Adelaide, South Australia, Australia. jessica.lindo@adelaide.edu.au
Jessica E
JE
Jackson
Zlatko
Z
Kopecki
Damian H
DH
Adams
Allison J
AJ
Cowin
D000199:Actins / Q000276:immunology*; D000818:Animals; D057134:Antibodies, Neutralizing / Q000378:metabolism*; D002352:Carrier Proteins; D049109:Cell Proliferation; D002921:Cicatrix / Q000276:immunology / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000276:immunology*; D004847:Epithelial Cells / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins; D013552:Swine; D013997:Time Factors; D015534:Trans-Activators; D014945:Wound Healing / Q000276:immunology; D014947:Wounds and Injuries / Q000276:immunology / Q000473:pathology*
D000199:Actins; D057134:Antibodies, Neutralizing; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C000609263:FlII protein, mouse; D008840:Microfilament Proteins; D015534:Trans-Activators; D003094:Collagen