tlpA gene expression is required for arginine and bicarbonate chemotaxis in Helicobacter pylori.
About half of the human population is infected with Helicobacter pylori, a bacterium causing gastritis, peptic ulcer and progression to gastric cancer. Chemotaxis and flagellar motility are required for colonization and persistence of H. pylori in the gastric mucus layer. It is not completely clear which chemical gradients are used by H. pylori to maintain its position. TlpA, a chemotaxis receptor for arginine/ bicarbonate, has been identified. This study aimed to find out whether tlpA gene expression is required for the chemotactic response to arginine/bicarbonate. Wild-type motile H. pylori ATCC 700392 and H. pylori ATCC 43504, a strain having an interrupted tlpA gene, were used. Also, a tlpA-knockout mutant of H. pylori 700392 (H. pylori 700-tlpA::cat) was produced by homologous recombination. Expression of tlpA was assessed by a Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) assay. Chemotaxis was measured as a Relative Chemotaxis Response (RCR) by a modified capillary assay. H. pylori 700392 presented chemotaxis to arginine and sodium bicarbonate. H. pylori 700-tlpA::cat showed neither tlpA gene expression nor chemotaxis towards arginine and bicarbonate. Besides confirming that TlpA is a chemotactic receptor for arginine/bicarbonate in H. pylori, this study showed that tlpA gene expression is required for arginine/bicarbonate chemotaxis.
Department of Neurobiology, Physiology and Behavior, University of California, Davis, CA 95616-8519, USA.
Oscar A
OA
Cerda
Felipe
F
Núñez-Villena
Sarita E
SE
Soto
José Manuel
JM
Ugalde
Remigio
R
López-Solís
Héctor
H
Toledo
D001120:Arginine / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D001639:Bicarbonates / Q000494:pharmacology*; D002633:Chemotaxis / Q000235:genetics*; D015870:Gene Expression; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D000072236:Methyl-Accepting Chemotaxis Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014508:Urea / Q000378:metabolism
D001426:Bacterial Proteins; D001639:Bicarbonates; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D014508:Urea; D001120:Arginine