Mechanism of the prokaryotic transmembrane disulfide reduction pathway and its in vitro reconstitution from purified components.
Making your (Dsb) connection: the redox pathway bringing reducing equivalents from bacterial cytoplasm, across the inner membrane, to the three reductive Dsb pathways in the otherwise oxidizing periplasm (see scheme; TR=thioredoxin reductase, Trx=thioredoxin) is reconstituted from purified components. Transfer of reducing equivalents across the membrane is demonstrated and underlying mechanistic details are revealed.
Institute for Molecular Biology and Biophysics, ETH Zurich, Switzerland. malojcic@fas.harvard.edu
Goran
G
Malojčić
Eric R
ER
Geertsma
Maurice S
MS
Brozzo
Rudi
R
Glockshuber
D002462:Cell Membrane / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004220:Disulfides / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism*; D010084:Oxidation-Reduction; D019897:Periplasm / Q000378:metabolism*; D011510:Proteolipids / Q000378:metabolism; D015398:Signal Transduction; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism*
D004220:Disulfides; D011510:Proteolipids; C020485:proteoliposomes; D013879:Thioredoxins; D013880:Thioredoxin-Disulfide Reductase