Genetic transformation mediated by piggyBac in the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae).
The Asian corn borer, Ostrinia furnacalis, is a serious pest of corn, sorghum, and cotton in China and other Asian countries. The present study is the first attempt to establish the transgenic line in O. furnacalis using a piggyBac transposon, which will shed light on the future genetic control of O. furnacalis. A piggyBac vector pBac[A3EGFP] was constructed to express enhanced green fluorescence protein (EGFP)under the control of Bombyx mori actin3 promoter. Transient EGFP expression was detected 48 h after preblastodermic microinjection of pBac[A3EGFP] and the excision assay showed the transgenic vector was precisely excised. In G1 animals, PCR (polymerase chain reaction)-based investigations revealed that the exogenous gene had been introduced into O. furnacalis genome and expressed at the transcriptional level. Western blot analysis showed EGFP expression at the protein level, indicating the heritability of the transgene.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Dan
D
Liu
Shanchun
S
Yan
Yongping
Y
Huang
Anjiang
A
Tan
David W
DW
Stanley
Qisheng
Q
Song
D000199:Actins / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D004625:Embryo, Nonmammalian; D005260:Female; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008845:Microinjections; D009036:Moths / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic; D019076:Transgenes
D000199:Actins; D004251:DNA Transposable Elements; D019476:Insect Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins