[Anti-tumor peptide of tumstatin in the treatment of the transplantable model of human laryngeal squamous carcinoma in nude mice].
OBJECTIVE
To study the effect of anti-tumor peptide of tumstatin on tumor growth of human laryngeal squamous carcinoma in nude mice and the underlying mechanism.


METHODS
Nude mice model bearing laryngocarcinoma were established by using human laryngeal squamous carcinoma cell line (Hep-II). The animals were given tumstatin or PBS for 10 consecutive days. The volumes of the subcutaneous tumor were observed. The microstructure in which the general 2-step immunohistochemical examination was adopted and ultra-micro-structural changes of carcinoma after administration of tumstatin were observed under light and electron microscopes for pathology examination.


RESULTS
The differences was statistically significant in the net mice weight, tumor weight, tumor volume and tumor weight/net mice weight between the treatment group and the control group (P<0.01). The restrained percentage of tumor was 51.58%. The necrosis and apoptosis of the tumor cells and the angiogenesis reduction were found under light and electron microscope in the treatment group. MVD of the treatment group was lower than that of the control group (P<0.01).


CONCLUSIONS
Tumstatin can significantly restrain the development of laryngocarcinoma.
Department of Otorhinolaryngology-Head and Neck Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China. hljwangchao@yahoo.com.cn
Chao
C
Wang
Qiuying
Q
Li
Hongchao
H
Yao
Ming
M
Liu
Yuli
Y
Xiao
Dejun
D
Jin
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001324:Autoantigens / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D024141:Collagen Type IV / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010455:Peptides / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
D001324:Autoantigens; D024141:Collagen Type IV; D010455:Peptides; C057112:type IV collagen alpha3 chain