Defining and detecting crossover-interference mutants in yeast.
The analysis of crossover interference in many creatures is complicated by the presence of two kinds of crossovers, interfering and noninterfering. In such creatures, the values of the traditional indicators of interference are subject not only to the strength of interference but also to the relative frequencies of crossing over contributed by the two kinds. We formalize the relationship among these variables and illustrate the possibilities and limitations of classical interference analysis with meiotic tetrad data from wild-type Saccharomyces cerevisiae and from mlh1 and ndj1 mutants.
Institute of Molecular Biology, University of Oregon, Eugene, Oregon, United States of America. fstahl@uoregon.edu
Frank
F
Stahl
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics; D003434:Crossing Over, Genetic / Q000502:physiology*; D008540:Meiosis / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics; D008954:Models, Biological; D000070957:MutL Protein Homolog 1; D009154:Mutation / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; C530079:CSM4 protein, S cerevisiae; D018797:Cell Cycle Proteins; C088859:MLH1 protein, S cerevisiae; D008565:Membrane Proteins; C433703:Ndj1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000070957:MutL Protein Homolog 1