Oral administration of oleic or linoleic acids modulates the production of inflammatory mediators by rat macrophages.
Oleic (OLA) and linoleic (LNA) acids are commonly consumed fatty acids and they can modulate the inflammatory response, in which macrophages play an important role. The aim of this study was to investigate the effects of these two fatty acids on the production of inflammatory mediators by macrophages. Rats received oral administration of water (control), OLA or LNA (0.22 g/kg body weight) daily for 10 days and peritoneal resident macrophages were then isolated. Subsequently, they were seeded in culture plates and the production of various inflammatory mediators was assessed. Oral administration with OLA decreased the production of IL-1β, IL-6 and CINC-2αβ by resident macrophages and LNA decreased the production of IL-1β, IL-6 and VEGF in the absence of lipopolysaccharide (LPS), although it accelerated IL-1β release and decreased IL-10 synthesis when cells were stimulated with LPS. Neither fatty acid affected the production of superoxide anion, hydrogen peroxide, nitrite, TNF-α, PGE(2), LTB(4) or 15(S)-HETE. Thus, OLA and LNA influence the production of several inflammatory mediators by macrophages.
Departamento de Fisiologia e Biofísica, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 1524, Cidade Universitária, Sao Paulo, SP, Brazil. jumagdalon@ig.com.br
Juliana
J
Magdalon
Marco A R
MA
Vinolo
Hosana G
HG
Rodrigues
Vivian A
VA
Paschoal
Rosângela P
RP
Torres
Jorge
J
Mancini-Filho
Philip C
PC
Calder
Elaine
E
Hatanaka
Rui
R
Curi
D000818:Animals; D019743:Chemokines, CXC / Q000096:biosynthesis; D018836:Inflammation Mediators / Q000378:metabolism*; D016753:Interleukin-10 / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008041:Linoleic Acids / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D019301:Oleic Acid / Q000494:pharmacology*; D051381:Rats; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
D019743:Chemokines, CXC; C513294:Cxcl3 protein, rat; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D008041:Linoleic Acids; D008070:Lipopolysaccharides; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D016753:Interleukin-10; D019301:Oleic Acid