Fusarium tupiense sp. nov., a member of the Gibberella fujikuroi complex that causes mango malformation in Brazil.
Fusarium tupiense, the main causal agent of mango malformation in Brazil, is described through a combination of morphological, biological and molecular markers. This new species belongs to the Gibberella fujikuroi species complex (GFSC) and has an anamorph morphologically similar to Fusarium mangiferae and F. sterilihyphosum. F. tupiense can be differentiated from other species in the G. fujikuroi species complex on the basis of sexual crosses, amplified fragment length polymorphism (AFLP) markers and partial sequences of the tef1 and tub2 genes. Female fertility for field isolates of F. tupiense appears to be low. PCR with primers specific for the mating type (MAT) alleles and sexual crosses identified this species as heterothallic with two idiomorphs. Female-fertile tester strains were developed for the identification of field strains of this species through sexual crosses.
Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco, Garanhuns, Pernambuco, Brazil. ludwig@ufla.br
Cristiano S
CS
Lima
Ludwig H
LH
Pfenning
Sarah S
SS
Costa
Lucas M
LM
Abreu
John F
JF
Leslie
D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D001938:Brazil; D003433:Crosses, Genetic; D004271:DNA, Fungal / Q000235:genetics; D005670:Fusarium / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D049770:Genes, Mating Type, Fungal / Q000235:genetics; D005874:Gibberella / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D056626:Inflorescence / Q000382:microbiology; D031022:Mangifera / Q000382:microbiology*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018520:Plant Shoots / Q000382:microbiology; D013172:Spores, Fungal / Q000145:classification / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification
D004271:DNA, Fungal