Morphological phenotypes of olfactory ensheathing cells display different migratory responses upon Slit-2.
Olfactory ensheathing cells (OECs) are a type of glial cells with morphological plasticity in the olfactory system. Cultured OECs display the process-bearing and flattened shape. Our previous studies have shown that the frontal application of Slit-2 gradient induced the collapse of leading front, and reversed the soma translocation of process-bearing OECs. However, the migratory properties of flattened OECs upon Slit-2 gradient remain elusive. Here, we found that Slit-2 gradient induced the collapse of their plasma membrane, and inhibited migration of flattened OECs. Upon to Slit-2 gradient, the leading front of flattened type 1 OECs firstly showed collapse and retraction, then gradually re-grew a new lamellipodia, finally, showed collapse again (this phenomenon was called as adaptation), while flattened type 2 OECs only showed collapse of plasma membrane. These different migratory responses upon Slit-2 stimulation were possibly due to their different sub-cellular distribution of Robo receptor. Furthermore, F-actin at the peripheral region of leading front was more sensitive to the Slit-2 stimulation than microtubules and the loss of F-actin might be implicated in initiating the collapse of flattened OECs. Finally, the adaptation of flattened type 1 OECs induced by Slit-2 was independent on protein synthesis. Taken together, these results demonstrate that morphological phenotypes of OECs display different migratory properties upon Slit-2 and an unexpected finding that the protein synthesis-independent adaptation in OECs induced by Slit-2.
School of Laboratory Medicine and Life Science, Wenzhou Medical College, Wenzhou, Zhejiang 325035, China.
Ying
Y
Wang
Zhi-hui
ZH
Huang
D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D016764:Cell Polarity; D048430:Cell Shape / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology* / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology* / Q000502:physiology*; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D009833:Olfactory Pathways / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D013347:Subcellular Fractions / Q000378:metabolism; D000098552:Slit Homolog 2 Protein; D000097905:Roundabout Proteins
D003513:Cycloheximide; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D011500:Protein Synthesis Inhibitors; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D000098552:Slit Homolog 2 Protein; C516057:Robo2 protein, rat; D000097905:Roundabout Proteins