Effect of HSP70 and 90 in modulation of JNK, ERK expression in preeclamptic placental endothelial cell.
Preeclampsia is a disease of worldwide significance with increasing maternal mortality rate of 20-80 %. Though apoptosis is a normal constituent during pregnancy, there seems to be an altered balance between proliferation and apoptosis of endothelial cell in preeclampsia leading to a placental dysregulation resulting in premature delivery. Molecular chaperones like HSP70 and 90 play a significant role in control of preeclamptic progression and protect the developing fetus. This is governed by alterations in expression of HSF1, HIF1α a nuclear transcription factor and signaling molecule like ERK, JNK1/2, and Bcl-2. Endothelial cell from normotensive and preeclamptic placenta were analyzed for variation in viability and expression of signaling molecules. A significant decrease in viability of endothelial cell (p < 0.05) was noted in preeclamptic samples when compared to normotensive samples. The results indicate that there was an increase in, HSP70 and 90 (p < 0.01), HSF1 (p < 0.01), HIF1α (p < 0.05), ERK (p < 0.05), JNK1/2 (p < 0.05), and Bcl-2 (p < 0.05). Though there is a significant change in the viability of endothelial cell, the live fetal delivery is not predominantly affected during preeclampsia. The interplay between these signaling molecules which alter the apoptotic pathway to sustain endothelial cell viability is discussed.
P.G. Department of Biochemistry, Bharathi Women's College, Affiliated to University of Madras, Chennai, Tamil Nadu, India. ntrfbwc@gmail.com
Ekambaram
E
Padmini
Venkatraman
V
Uthra
Srinivasan
S
Lavanya
D000328:Adult; D002470:Cell Survival; D042783:Endothelial Cells / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D018384:Oxidative Stress; D010920:Placenta / Q000473:pathology*; D011225:Pre-Eclampsia / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy
D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases