Entrapment of Saccharomyces cerevisiae and 3T3 fibroblast cells into blue light cured hydrogels.
Hydrogels, containing yeast cells or fibroblast cells, were fabricated using blue light-induced polymerization technique. The cell-loaded prepolymer formulation was comprised of poly(ethyleneglycol) diacrylate (more than or equal to 50% v/v), 0.5 wt % Eosin Y and 0.5 wt % triethanolamine as the base oligomer, photo-initiator, and co-initiator, respectively. The two model cell lines, Saccharomyces cerevisiae and NIH 3T3 fibroblasts maintained high viability pre- and post-processing. Several bioassays have demonstrated the unaffected intracellular and extracellular activities of the cells entrapped within the hydrogels. Scanning electron microscopy confirmed the proliferation of S. cerevisiae cells that were entrapped and cultivated for 48 h in growth media, which validated the favorable microenvironment and nutrient transport in these gels. Upon entrapment, fibroblast cells remain viable upto 12 h, however they failed to attach within the crosslinked network, thus no further proliferation was observed. The tunable properties of this hydrogel system project it as a useful matrix for specialized biohybrids.
Department of Bioengineering, University of Massachusetts Dartmouth, N. Dartmouth, Massachusetts 02747, USA. swatimishra.iitd@gmail.com
Swati
S
Mishra
Frank J
FJ
Scarano
Paul
P
Calvert
D016475:3T3 Cells; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004058:Diffusion / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D020100:Hydrogels / Q000494:pharmacology* / Q000528:radiation effects*; D007700:Kinetics; D008027:Light; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects; D018613:Microscopy, Confocal; D011092:Polyethylene Glycols / Q000737:chemistry; D011485:Protein Binding / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013395:Sucrose / Q000378:metabolism; D013997:Time Factors; D043324:beta-Fructofuranosidase / Q000378:metabolism
D003432:Cross-Linking Reagents; D020100:Hydrogels; C437167:poly(ethylene glycol)diacrylate; D011092:Polyethylene Glycols; D013395:Sucrose; D043324:beta-Fructofuranosidase