Trifluoroethanol and acetonitrile induced formation of the molten globule states and aggregates of cellulase.
A systematic investigation on the effects of trifluoroethanol and acetonitrile at various concentrations on cellulase (EC 3.2.1.4) was studied by enzyme assay, intrinsic fluorescence, ANS binding, circular dichroism and ATR-Fourier transform infra red spectroscopy. The results show the presence of molten globule states at 3% (v/v) TFE and 80% (v/v) ACN. Cellulase aggregates at 25% (v/v) TFE and 90% (v/v) ACN, as detected by decrease in intrinsic and ANS fluorescence, loss in tertiary structure and enzyme activity, increase non-native β-sheet structure as evident from far-UV CD and FTIR spectra, increase in Thioflavin T fluorescence and shift in Congo red assay.
Department of Biochemistry, Faculty of Life Science, Aligarh Muslim University, Aligarh, India.
Afshin
A
Iram
Aabgeena
A
Naeem
D000097:Acetonitriles / Q000494:pharmacology*; D001234:Aspergillus niger / Q000201:enzymology; D052160:Benzothiazoles; D032701:Biomimetics; D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D003224:Congo Red / Q000737:chemistry; D011487:Protein Conformation / Q000187:drug effects; D055503:Protein Multimerization / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared; D013844:Thiazoles / Q000737:chemistry; D014270:Trifluoroethanol / Q000494:pharmacology*
D000097:Acetonitriles; D052160:Benzothiazoles; D013844:Thiazoles; C009462:thioflavin T; D003224:Congo Red; D014270:Trifluoroethanol; D002480:Cellulase; C032159:acetonitrile