Enhanced the enzymatic hydrolysis efficiency of wheat straw after combined steam explosion and laccase pretreatment.
Laccase, capable of selectively degrading lignin while keeping cellulose intact, has been widely applied for the modification and bio-bleaching of pulp. In this study Sclerotium sp. laccase (MSLac) was employed in combination with steam explosion to evaluate the effect of this treatment on cellulose hydrolysis. Combined steam explosion with laccase pretreatment enhanced the cellulose conversion rate of wheat straw no matter in the case of successive (MSLac-Cel) and simultaneous (MSLac+Cel) MSLac and cellulase hydrolysis. The highest cellulose conversion rate of 84.23% was obtained when steam-exploded wheat straw (SEWS) (1.3 MPa, 5 min) was treated by MSLac+Cel at a laccase loading of 0.55 U g(-1) substrate. FT-IR and SEM analyses indicated that MSLac oxidized the phenol and changed electron configuration of the ring, which contributed to loosening the compact wrap of lignin-carbohydrate complex and consequently enhancing the enzymatic hydrolysis efficiency of cellulose. This article provided a promising method for lignocellulose bio-pretreatment.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Weihua
W
Qiu
Hongzhang
H
Chen
D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D005107:Explosions; D006868:Hydrolysis / Q000187:drug effects; D042845:Laccase / Q000378:metabolism* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013227:Steam; D014908:Triticum / Q000737:chemistry / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis*
D013227:Steam; D014866:Waste Products; D002482:Cellulose; D042845:Laccase; D002480:Cellulase