[Expression of monocarboxylate transporter 8 mRNA in the brain tissue of rats with cerebral ischemia].
OBJECTIVE
To investigate the mRNA expression of monocarboxylate transporter 8 (MCT8), a thyroid hormone transport protein, in the lateral ventricle of rats with cerebral ischemia.


METHODS
Immunofluorescence staining was used to observe the expression of MCT8 in the lateral ventricle of 5 normal SD rats. Another 20 adult male SD rats were randomized into 4 groups and subject to permanent ligation of both the common carotid arteries (2-vessel occlusion, 2VO) for 3 days, 2 weeks, or 5 weeks, or no ligation (control). At the end of the experiment, the transcriptional level of MCT8 in the brain tissue of the rats were detected using fluorescent quantitative PCR.


RESULTS
MCT8 mRNA levels in 3-day and 2-week 2VO groups were comparable with that in the control group (P=0.909; P=0.694), but increased significantly in 5-week 2VO group compared with that in the control and 3-day 2VO groups (P=0.029; P=0.023). No significance was found in MCT8 mRNA between the 2-week and 5-week 2VO groups (P=0.065).


CONCLUSIONS
Prolonged cerebral ischemia causes compensatory increase of MCT8 mRNA expression on the capillary endothelial cell membranes in the lateral ventricle of rats.
Department of Neurology, Southern Medical University, Guangzhou, China. cuidan197936@163.com
Dan
D
Cui
Yuqing
Y
Guan
Haishan
H
Jang
Jin
J
Wang
Lei
L
Xi
Qun
Q
Wang
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D002544:Cerebral Infarction / Q000378:metabolism; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D027501:Monocarboxylic Acid Transporters; C478998:monocarboxylate transporter 8, rat