Calf form bovine leukosis with lameness in a Holstein heifer.
A 12-month-old Holstein heifer with anorexia, lameness, and enlargement of peripheral lymph nodes was suspected of having bovine leukosis. Although lymphocytosis was not observed, cytology of fine needle aspirate from a superficial cervical node, and increased serum lactate dehydrogenase and thymidine kinase activities, strongly suggested lymphosarcoma. Increased numbers of mononuclear cells as well as mitotic cells were observed in synovial fluid collected from swollen joints. Pathological examination confirmed B-cell calf form bovine leukosis and joint swelling related to neoplastic cell infiltration. Both interleukin-2 receptor and thymidine kinase 1 genes were highly expressed in cells from superficial cervical lymph node aspirate.
Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan.
Mohammad Monir
MM
Tawfeeq
Saori
S
Miura
Yuuki
Y
Nakanishi
Kazuya
K
Sugimoto
Yoshiyasu
Y
Kobayashi
Hidefumi
H
Furuoka
Hisashi
H
Inokuma
D000818:Animals; D044963:Biopsy, Fine-Needle / Q000662:veterinary; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000150:complications / Q000276:immunology* / Q000473:pathology*; D017809:Fatal Outcome; D005260:Female; D006652:Histological Techniques / Q000662:veterinary; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007794:Lameness, Animal / Q000209:etiology / Q000276:immunology* / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013582:Synovial Fluid / Q000166:cytology / Q000276:immunology*; D013937:Thymidine Kinase / Q000097:blood
D015375:Receptors, Interleukin-2; D007770:L-Lactate Dehydrogenase; D013937:Thymidine Kinase