The role of tissue factor and protease-activated receptor 2 in endometriosis.
OBJECTIVE
Little is known about the roles of TF and PAR-2 in endometriosis. This article investigated the expression of TF and PAR-2 in ectopic and eutopic endometrium with endometriosis and their relationship with the menstrual cycle.


METHODS
Ectopic and eutopic endometrium tissues from 42 women with ovarian endometrioma and endometrium tissues from 20 women without endometriosis were obtained. All the samples were assessed for TF and PAR-2 protein location using immunohistochemistry and for relative TF and PAR-2 mRNA expression using real-time florescent quantitative polymerase chain reaction (FQ-PCR).


RESULTS
Total TF and PAR-2 expression were significantly higher in ectopic and eutopic endometrium of women with endometriosis when compared with controls. Moreover, TF expression in ectopic and eutopic endometrium and PAR-2 expression in ectopic endometrium were significantly increased through the whole menstrual cycle. However, in eutopic endometrium with endometriosis, PAR-2 expression only in secretory phase was higher than its cycle-matched normal controls. There is no such difference in the proliferative phase.


CONCLUSIONS
The abnormal co-upregulated expression of TF and PAR-2 in eutopic and ectopic endometrium may affect the development and growth of endometriotic lesions and highlighted the pathologic role of TF and PAR-2 in eutopic endometrium in endometriosis.
Department of Obstetrics and Gynaecology, Third Affiliated Hospital of Guangzhou Medical College, Guangzhou, China.
Min
M
Lin
Huinan
H
Weng
Xuefeng
X
Wang
Bing
B
Zhou
Ping
P
Yu
Yifeng
Y
Wang
D000328:Adult; D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008597:Menstrual Cycle / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D060888:Real-Time Polymerase Chain Reaction; D044464:Receptor, PAR-2 / Q000235:genetics*; D013925:Thromboplastin / Q000235:genetics*
D012333:RNA, Messenger; D044464:Receptor, PAR-2; D013925:Thromboplastin