Bread matters: a national initiative to profile the genetic diversity of Australian wheat.
The large and complex genome of wheat makes genetic and genomic analysis in this important species both expensive and resource intensive. The application of next-generation sequencing technologies is particularly resource intensive, with at least 17 Gbp of sequence data required to obtain minimal (1×) coverage of the genome. A similar volume of data would represent almost 40× coverage of the rice genome. Progress can be made through the establishment of consortia to produce shared genomic resources. Australian wheat genome researchers, working with Bioplatforms Australia, have collaborated in a national initiative to establish a genetic diversity dataset representing Australian wheat germplasm based on whole genome next-generation sequencing data. Here, we describe the establishment and validation of this resource which can provide a model for broader international initiatives for the analysis of large and complex genomes.
Australian Centre for Plant Functional Genomics and University of Queensland, St. Lucia, Qld, Australia.
David
D
Edwards
Stephen
S
Wilcox
Roberto A
RA
Barrero
Delphine
D
Fleury
Colin R
CR
Cavanagh
Kerrie L
KL
Forrest
Matthew J
MJ
Hayden
Paula
P
Moolhuijzen
Gabriel
G
Keeble-Gagnère
Matthew I
MI
Bellgard
Michał T
MT
Lorenc
Catherine A
CA
Shang
Ute
U
Baumann
Jennifer M
JM
Taylor
Matthew K
MK
Morell
Peter
P
Langridge
Rudi
R
Appels
Anna
A
Fitzgerald
D001315:Australia; D030541:Databases, Genetic; D014644:Genetic Variation; D018745:Genome, Plant; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics*