Effect of ribose, xylose, aspartic acid, glutamine and nicotinic acid on ethyl (S)-4-chloro-3-hydroxybutanoate synthesis by recombinant Escherichia coli.
Most reductases which belong to the short chain dehydrogenase/reductase (SDR) superfamily require NAD (P) H for activity. Addition of this cofactor was still necessary for the production of ethyl (S)-4-chloro-3-hydroxybutanoate by Escherichia coli even when a cofactor regeneration system was constructed by co-expressing carbonyl reductase from Pichia stipitis (PsCRI) and glucose dehydrogenase from Bacillus megaterium (BmGDH). In an attempt to reduce dependence on the expensive cofactor, compounds directly or indirectly involved in NADP synthesis were added to the medium. Only glutamine and xylose enhanced the content of intracellular NADP (H) and the concentration of product. The concentration and yield of (S)-CHBE reached 730 mM and 48.7%, with 30 g/L of glutamine and 40 g/L of xylose, a 2.6-fold increase over the control without the addition of the two compounds.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, PR China.
Nan
N
Li
Yueyuan
Y
Zhang
Qi
Q
Ye
Yuzhe
Y
Zhang
Yong
Y
Chen
Xiaochun
X
Chen
Jinglan
J
Wu
Jianxin
J
Bai
Jingjing
J
Xie
Hanjie
H
Ying
D001224:Aspartic Acid / Q000494:pharmacology*; D055162:Biocatalysis / Q000187:drug effects; D002087:Butyrates / Q000378:metabolism*; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D005973:Glutamine / Q000494:pharmacology*; D009525:Niacin / Q000494:pharmacology*; D011995:Recombination, Genetic / Q000187:drug effects / Q000235:genetics; D012266:Ribose / Q000494:pharmacology*; D014994:Xylose / Q000494:pharmacology*
D002087:Butyrates; D003470:Culture Media; C121244:ethyl 4-chloro-3-hydroxybutanoate; D005973:Glutamine; D009525:Niacin; D001224:Aspartic Acid; D012266:Ribose; D014994:Xylose