A loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: application to a field survey.
In this study, we applied a loop-mediated isothermal amplification method for identification of human Taenia tapeworms in Tibetan communities in Sichuan, China. Out of 51 proglottids recovered from 35 carriers, 9, 1, and 41 samples were identified as Taenia solium, Taenia asiatica and Taenia saginata, respectively. Same results were obtained afterwards in the laboratory, except one sample. These results demonstrated that the LAMP method enabled rapid identification of parasites in the field surveys, which suggested that this method would contribute to the control of Taenia infections in endemic areas.
Department of Parasitology, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, 078-8510, Hokkaido, Japan.
Agathe
A
Nkouawa
Yasuhito
Y
Sako
Tiaoying
T
Li
Xingwang
X
Chen
Minoru
M
Nakao
Tetsuya
T
Yanagida
Munehiro
M
Okamoto
Patrick
P
Giraudoux
Francis
F
Raoul
Kazuhiro
K
Nakaya
Ning
N
Xiao
Jiamin
J
Qiu
Dongchuan
D
Qiu
Philip S
PS
Craig
Akira
A
Ito
D000818:Animals; D002590:Cestode Infections / Q000453:epidemiology / Q000469:parasitology*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D013045:Species Specificity; D013621:Taenia / Q000145:classification* / Q000235:genetics*; D018609:Tibet / Q000453:epidemiology