Activity-based probes for studying the activity of flavin-dependent oxidases and for the protein target profiling of monoamine oxidase inhibitors.
High profile: new activity-based protein profiling (ABPP) probes have been designed that target exclusively monoamine oxidases A and B within living cells (see picture; FAD=flavin adenine dinucleotide, FMN=flavin monodinucleotide). With these probes it could be shown that the MAO inhibitor deprenyl, which is in clinical use against Parkinson's disease, shows unique protein specificity despite its covalent mechanism of action.
Institute of Organic Chemistry, Graz University of Technology, Austria.
Joanna M
JM
Krysiak
Johannes
J
Kreuzer
Peter
P
Macheroux
Albin
A
Hermetter
Stephan A
SA
Sieber
Rolf
R
Breinbauer
D001932:Brain Neoplasms / Q000188:drug therapy* / Q000201:enzymology*; D005486:Flavin Mononucleotide / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D006801:Humans; D008995:Monoamine Oxidase / Q000737:chemistry* / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D012642:Selegiline / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D008996:Monoamine Oxidase Inhibitors; D005182:Flavin-Adenine Dinucleotide; D012642:Selegiline; D005486:Flavin Mononucleotide; D008995:Monoamine Oxidase