G-rich sequence-specific recognition and scission of human genome by PNA/DNA hybrid G-quadruplex formation.
Hole in one: A single peptide nucleic acid (PNA) effectively targets the G-rich region in double-stranded DNA through formation of a PNA/DNA hybrid G-quadruplex. Only one target site in the whole human genome was selectively cleaved by the hybrid G-quadruplex. Such site-selective scission of DNA is central to gene manipulation for molecular biology, biotechnology, and therapy.
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Takumi
T
Ishizuka
Jie
J
Yang
Makoto
M
Komiyama
Yan
Y
Xu
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D053960:DNA Breaks; D054856:G-Quadruplexes; D015894:Genome, Human; D006801:Humans; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics
D020135:Peptide Nucleic Acids; D016271:Proto-Oncogene Proteins c-myc; D004247:DNA