Hydrolyzed caseinomacropeptide conjugated galactooligosaccharides support the growth and enhance the bile tolerance in Lactobacillus strains.
In this study bioactive caseinomacropeptide was conjugated with prebiotic galactooligosaccharides (hCMP:GOS) by Maillard reaction to synthesize value added prebiotic compounds to Lactobacillus strains. Growth study showed the ability of hCMP:GOS to serve as a sole carbon source for Lactobacillus strains. A significant amount of acetate and lactate was detected in cell free culture supernatant by HPLC. It demonstrated the ability of Lactobacillus strains to ferment the hCMP:GOS as a carbon source. In addition, hCMP:GOS grown Lactobacillus cells exhibited enhanced bile tolerance and retained 90% viability. Overall results of this study indicate that the hCMP conjugated GOS can be potential multipurpose prebiotic substrates to enhance the growth and bile tolerance in Lactobacillus strains and serve as a fermentable substrate to produce beneficial metabolites in the host.
Center for Food Safety-Department of Food Science, University of Arkansas , Fayetteville, Arkansas 72704, United States.
Arunachalam
A
Muthaiyan
Oswaldo
O
Hernandez-Hernandez
F Javier
FJ
Moreno
Maria Luz
ML
Sanz
Steven C
SC
Ricke
D001647:Bile Acids and Salts / Q000494:pharmacology*; D002364:Caseins / Q000737:chemistry* / Q000378:metabolism*; D005285:Fermentation; D006801:Humans; D006868:Hydrolysis; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D056692:Prebiotics / Q000032:analysis
D001647:Bile Acids and Salts; D002364:Caseins; D009844:Oligosaccharides; D010446:Peptide Fragments; D056692:Prebiotics; C065989:caseinomacropeptide; D019344:Lactic Acid