[Study of chemical kinetics between pyrroloquinoline quinine and D-serine in body by ion-pair liquid chromatography].
As a new neurotransmitter present in the glial cells, D-serine (DSer) plays an important role in central nervous system diseases. Pyrroloquinoline quinine (PQQ) can promote the production of the nerve growth factor and has a protective effect on nerve injuries. The chemical kinetics of PQQ and DSer was studied by determining the free contents of PQQ using ion-pair liquid chromatography (LC), so it can provide important information for the mechanisms of PQQ in the regulation of neurotransmitter. The PQQ and the production of the incubation were separated on an Amethyst C18-P column using tetrabutylammonium bromide as ion-pair reagent. The average recoveries were between 94.2% and 99.3%, and the relative standard deviations were between 1.05% and 2.03%. The average rate constants (K) of PQQ with DSer were 0.032, 0.07 and 0.17 h(-1) at 25, 37 and 50 degrees C, respectively. The average activation energy (E(a)) was 54.7 kJ/mol. The values of half life (t1/2) were 22.0, 9.8 and 3.99 h at 25, 37 and 50 degrees C, respectively. The results showed that PQQ can regulate the balance of DSer in the brain. The method is simple and reliable.
Jiangsu Institute of Nuclear Medicine, Key Laboratory of Nuclear Medicine of Ministry of Health, Key Laboratory of Molecular Nuclear Medicine of Jiangsu, Wuxi 214063, China. zhouxingqin@jsinm.org
Xingqin
X
Zhou
Xiaofeng
X
Qin
Jiankang
J
Zhang
Guoxian
G
Cao
D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007700:Kinetics; D045542:PQQ Cofactor / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D012694:Serine / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D013237:Stereoisomerism
D012694:Serine; D045542:PQQ Cofactor