Resveratrol induces antioxidant defence via transcription factor Yap1p.
Resveratrol is a polyphenol suggested to play a protective role against ageing and age-related diseases. We demonstrate that administering low-doses of resveratrol causes ROS accumulation and transcriptional changes in yeast cells and human adipocytes. These changes in gene expression depend on the oxidative transcription factor Yap1p. In particular, resveratrol induces expression of Yap1p gene targets, such as TRX2, TRR1 or AHP1, in a Yap1p-dependent mode. Under resveratrol treatment, Yap1p is phosphorylated and accumulated in the nucleus. Yap1p knockout causes resveratrol sensitivity, which totally depends on the presence of the C-terminal region of Yap1p. Thus, resveratrol may enhance cellular lifespan by hormetic ROS accumulation, which leads to strengthening the cells' antioxidant capacity.
Hospital Universitari de Tarragona Joan XXIII. IISPV, Universitat Rovira i Virgili, CIBERDEM, Spain. xescote@gmail.com
Xavier
X
Escoté
Merce
M
Miranda
Sandra
S
Menoyo
Boris
B
Rodríguez-Porrata
Didac
D
Carmona-Gutiérrez
Helmut
H
Jungwirth
Frank
F
Madeo
Ricardo R
RR
Cordero
Albert
A
Mas
Francisco
F
Tinahones
Josep
J
Clotet
Joan
J
Vendrell
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000077185:Resveratrol; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D013267:Stilbenes; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; D000077185:Resveratrol