The respiratory burst activity and expression of catalase in white shrimp, Litopenaeus vannamei, during long-term exposure to pH stress.
In this study, changes of reactive oxygen species (ROS) and the mRNA expression of catalase of the Pacific white shrimp, Litopenaeus vannamei, exposed to pH (5.4, 6.7, 8.0, and 9.3) stress was investigated at different stress time (24, 48, 72, 96, and 120 h). Level of malondialdehyde (MDA) in shrimp also were assessed. The results revealed that acidic (pH 5.4 and 6.7) or alkaline exposure (pH 9.3) induced production of ROS hemocytes and increase of MDA level in shrimp. Moreover, the catalase mRNA expression in hepatopancreas of L. vannamei was up-regulated in 24 h at pH 5.4, in 72 h at pH 6.7 and in 48 h at pH 9.3, whereas was down-regulated significantly after 72 h acidic (pH 5.4 and 6.7) or alkaline (pH 9.4) exposure. In the present study, there was the relationship between ROS and catalase mRNA expression under normal acidic and alkaline conditions. At pH 8, the increase of catalase transcripts due to up-regulation by ROS, whereas MDA level did not significantly change, suggesting activation of corresponding protective mechanisms of detoxifying ROS is essential for the proper functioning of cells and the survival of shrimps.
Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou, PR China. weina63@yahoo.com.cn
Wei-Na
WN
Wang
Bao-Sheng
BS
Li
Jin-Jian
JJ
Liu
Lei
L
Shi
M J
MJ
Alam
Shi-Juan
SJ
Su
Juan
J
Wu
Lei
L
Wang
An-Li
AL
Wang
D000818:Animals; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D004781:Environmental Exposure / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008315:Malondialdehyde / Q000378:metabolism; D033561:Penaeidae / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological; D015854:Up-Regulation
D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D002374:Catalase