Determination of posttranslational modifications of photoreceptor differentiation factor NRL: focus on SUMOylation.
Conjugation of SUMO (small ubiquitin-related modifier 1) is a critical posttranslational modification, with significant impact on protein function/activity. Here, we describe direct SUMOylation of GST (glutathione S-transferase)-fusion protein and immunoprecipitation assays for investigating SUMOylation of any protein of interest. We have employed these methods to examine SUMOylation of the basic-motif leucine zipper transcription factor NRL.
Neurobiology Neurodegeneration and Repair Laboratory, National Eye Institute, National Institutes of Health, Bethesda, MD, USA.
Jerome E
JE
Roger
Jacob
J
Nellissery
Anand
A
Swaroop
D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism*; D002460:Cell Line; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D058207:Sumoylation
D050976:Basic-Leucine Zipper Transcription Factors; D005136:Eye Proteins; C490037:NRL protein, human; D011993:Recombinant Fusion Proteins