Porous polysaccharide-based scaffolds for human endothelial progenitor cells.
Human ECFCs contribute to vascular repair. For this reason, they are considered as valuable cell therapy products in ischemic diseases. Porous scaffolds are prepared that are composed of natural polysaccharides, pullulan and dextran, by chemical crosslinking without use of organic solvents. These porous scaffolds, which have pores with an average size of 42 µm and a porosity of 21%, preserve the viability and the proliferation of cord-blood ECFCs. After 7 d of culture in porous scaffolds, ECFCs express endothelial markers (CD31 and vWf) and maintain endothelial functions. The cultured cells can be easily retrieved by enzymatic degradation of the porous scaffolds. In vitro results suggest that the porous scaffold could allow cell delivery of ECFCs for treatment of vascular diseases.
Inserm, U972, Hôpital Paul Brousse, Villejuif, France.
Mélanie
M
Lavergne
Mohammed
M
Derkaoui
Catherine
C
Delmau
Didier
D
Letourneur
Georges
G
Uzan
Catherine
C
Le Visage
D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D015415:Biomarkers / Q000032:analysis; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D003911:Dextrans / Q000737:chemistry*; D005434:Flow Cytometry; D005936:Glucans / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D016062:Porosity; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014841:von Willebrand Factor / Q000032:analysis
D001672:Biocompatible Materials; D015415:Biomarkers; D003432:Cross-Linking Reagents; D003911:Dextrans; D005936:Glucans; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014841:von Willebrand Factor; C009109:pullulan