Correlation of dynamic PET and gene array data in patients with gastrointestinal stromal tumors.
BACKGROUND
The results obtained with dynamic PET (dPET) were compared to gene expression data obtained in patients with gastrointestinal stromal tumors (GIST). The primary aim was to assess the association of the dPET results and gene expression data.


METHODS
dPET was performed following the injection of F-18-fluorodeoxyglucose (FDG) in 22 patients with GIST. All patients were examined prior to surgery for staging purpose. Compartment and noncompartment models were used for the quantitative evaluation of the dPET examinations. Gene array data were based on tumor specimen obtained by surgery after the PET examinations.


RESULTS
The data analysis revealed significant correlations for the dPET parameters and the expression of zinc finger genes (znf43, znf85, znf91, znf189). Furthermore, the transport of FDG (k1) was associated with VEGF-A. The cell cycle gene cyclin-dependent kinase inhibitor 1C was correlated with the maximum tracer uptake (SUVmax) in the tumors.


CONCLUSIONS
The data demonstrate a dependency of the tracer kinetics on genes associated with prognosis in GIST. Furthermore, angiogenesis and cell proliferation have an impact on the tracer uptake.
Medical PET Group, Biological Imaging, CCU Nuclear Medicine, German Cancer Research Center, 69120 Heidelberg, Germany. lgs@ads-lgs.de
Ludwig G
LG
Strauss
Antonia
A
Dimitrakopoulou-Strauss
Dirk
D
Koczan
Leyun
L
Pan
Peter
P
Hohenberger
D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D005770:Gastrointestinal Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism*; D046152:Gastrointestinal Stromal Tumors / Q000000981:diagnostic imaging / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D049268:Positron-Emission Tomography / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013223:Statistics as Topic
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18