Expression of CCL1 and CCL18 in atopic dermatitis and psoriasis.
BACKGROUND
Recent studies have shown that chemoattractive proteins play an important role in the organization of the innate and adaptive immune responses. There are some reports that chemokine (C-C motif) ligand (CCL)1 and CCL18, members of a family of chemoattractive proteins, have increased expression in atopic dermatitis (AD).


OBJECTIVE
To evaluate the quantity and pattern of CCL1 and CCL18 expression in lesions and blood of patients with AD, and compare them with those of patients with psoriasis.


METHODS
Biopsy specimens were taken from atopic skin and normal-appearing skin of patients with AD and from the psoriatic skin only of patients with psoriasis. Quantitative real-time PCR analysis and immunohistochemistry of CCL1 and CCL18 expression were performed, and the quantities of expressed CCL1 and CCL18 in acute AD were compared with those of normal-appearing atopic skin and psoriatic skin. The serum level of CCL1 and CCL18 was assessed by ELISA.


RESULTS
Expression of CCL1 mRNA and protein was significantly higher in the acute lesional skin of patients with AD than in their nonlesional skin or in the lesional skin of patients with psoriasis. Both CCL18 mRNA and protein were abundant in acute AD lesions and in psoriatic lesions, but were lower in the nonlesional skin of patients with AD. The serum levels of CCL1 and CCL18 were not different in patients with AD and patients with psoriasis.


CONCLUSIONS
CCL1 is a chemokine that is associated with AD. Both CCL1 and CCL18 may play important roles in the initiation and progression of atopic skin inflammation.
Department of Dermatology, Collage of Medicine, Hallym University, Seoul, Korea.
H O
HO
Kim
S I
SI
Cho
B Y
BY
Chung
H K
HK
Ahn
C W
CW
Park
C H
CH
Lee
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D054402:Chemokine CCL1 / Q000378:metabolism*; D019742:Chemokines, CC / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012720:Severity of Illness Index; D055815:Young Adult
C065971:CCL1 protein, human; C111792:CCL18 protein, human; D054402:Chemokine CCL1; D019742:Chemokines, CC