Magneto-controlled electrochemical immunoassay of brevetoxin B in seafood based on guanine-functionalized graphene nanoribbons.
A facile and feasible magneto-controlled immunosensing platform was designed for sensitive electrochemical immunoassay of brevetoxin B (BTX-2) in seafood by using guanine-assembled graphene nanoribbons (GGNRs) as molecular tags on a home-made magnetic carbon paste electrode. Initially, monoclonal mouse anti-BTX-2 antibodies were covalently immobilized on the surface of magnetic beads, which were used as the immunosensing probes for the capture of BTX-2. The recognition elements were prepared by chemical modification of bovine serum albumin-BTX-2 conjugates (BTX-2-BSA) with the GGNRs. Based on a competitive-type immunoassay format, the formed magnetic immunocomplex was integrated on the electrode with an external magnet, followed by determination in pH 6.5 phosphate-buffered solution containing 2 μM Ru(bpy)(3)Cl(2). Under optimal conditions, the electrochemical signals decreased with the increasing BTX-2 concentrations in the sample. The dynamic concentration range spanned from 1.0 pg mL(-1) to 10 ng mL(-1) with a detection limit of 1.0 pg mL(-1) BTX-2. Inter- and intra-batch assay precisions were substantially improved by resorting to the GGNR manifold. The method featured unbiased identification of negative (blank) and positive samples. No significant differences at the 95% confidence level were encountered in the analysis of 12 spiked samples including S. constricta, M. senhousia and T. granosa between the electrochemical immunoassay and commercially available enzyme-linked immunosorbent assay (ELISA) for determination of BTX-2.
Ministry of Education & Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
Juan
J
Tang
Li
L
Hou
Dianping
D
Tang
Jun
J
Zhou
Zhouping
Z
Wang
Jianrong
J
Li
Guonan
G
Chen
D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D004141:Dinoflagellida / Q000302:isolation & purification*; D055664:Electrochemical Techniques / Q000379:methods; D006108:Graphite / Q000737:chemistry; D006147:Guanine / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry; D008387:Marine Toxins / Q000032:analysis* / Q000276:immunology; D008974:Mollusca / Q000469:parasitology*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D035964:Oxocins / Q000032:analysis* / Q000276:immunology; D012758:Shellfish / Q000032:analysis / Q000469:parasitology*; D000095702:Polyether Toxins
D055910:Antibodies, Immobilized; D006108:Graphite; D006147:Guanine; D008387:Marine Toxins; D037742:Nanotubes, Carbon; D035964:Oxocins; C043466:brevetoxin B; D000095702:Polyether Toxins