A histone acetyltransferase regulates active DNA demethylation in Arabidopsis.
Active DNA demethylation is an important part of epigenetic regulation in plants and animals. How active DNA demethylation is regulated and its relationship with histone modification patterns are unclear. Here, we report the discovery of IDM1, a regulator of DNA demethylation in Arabidopsis. IDM1 is required for preventing DNA hypermethylation of highly homologous multicopy genes and other repetitive sequences that are normally targeted for active DNA demethylation by Repressor of Silencing 1 and related 5-methylcytosine DNA glycosylases. IDM1 binds methylated DNA at chromatin sites lacking histone H3K4 di- or trimethylation and acetylates H3 to create a chromatin environment permissible for 5-methylcytosine DNA glycosylases to function. Our study reveals how some genes are indicated by multiple epigenetic marks for active DNA demethylation and protection from silencing.
Shanghai Center for Plant Stress Biology and Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, China.
Weiqiang
W
Qian
Daisuke
D
Miki
Heng
H
Zhang
Yunhua
Y
Liu
Xi
X
Zhang
Kai
K
Tang
Yunchao
Y
Kan
Honggui
H
La
Xiaojie
X
Li
Shaofang
S
Li
Xiaohong
X
Zhu
Xiaobing
X
Shi
Kangling
K
Zhang
Olga
O
Pontes
Xuemei
X
Chen
Renyi
R
Liu
Zhizhong
Z
Gong
Jian-Kang
JK
Zhu
D000107:Acetylation; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D045647:DNA Glycosylases / Q000378:metabolism; D019175:DNA Methylation; D018744:DNA, Plant / Q000378:metabolism*; D020868:Gene Silencing; D017343:Genes, Plant; D051548:Histone Acetyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D008745:Methylation; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D019076:Transgenes
D029681:Arabidopsis Proteins; D002843:Chromatin; D018744:DNA, Plant; D006657:Histones; D009687:Nuclear Proteins; C471271:ROS1 protein, Arabidopsis; D051548:Histone Acetyltransferases; C573241:IDM1 protein, Arabidopsis; C093335:5-methylcytosine-DNA glycosylase; D045647:DNA Glycosylases