Effects of coumestrol administration to maternal mice during pregnancy and lactation on renal Ca metabolism in neonatal mice.
The present study was conducted to clarify the effects of coumestrol administration to maternal mice during pregnancy and lactation on serum Ca and Ca metabolism in their neonatal mice. From 6.5 to 16.5 days post coitus and from 3 to 10 days after parturition, maternal mice were administered at 200 µg/kg body weight/day of coumestrol. Coumestrol administration did not affect weight gains, serum Ca and the expression of vitamin D receptor (VDR) protein in the kidney of neonatal mice, but weight gains of maternal mice were decreased by coumestrol administration. Coumestrol administration increased the messenger RNA (mRNA) expressions of epithelial Ca channels 1 (ECaC1) and VDR in the kidney of neonatal mice, and also increased the mRNA expressions of ECaC2 in the kidney and small intestine of male neonatal mice. The mRNA expressions of ECaC1, ECaC2, calbindin-D(9k) (CaBP-9k) and estrogen receptor (ER)α in the kidney and VDR in the small intestine of male neonatal mice were higher than those of female mice. Thus, coumestrol administration to maternal mice during pregnancy and lactation may affect renal Ca metabolism in neonatal mice, especially male neonatal mice via maternal milk.
Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, Japan.
Michihisa
M
Ueda
Yoshihiro
Y
Horiguchi
Miki
M
Sugimoto
Shuntaro
S
Ikeda
Shinichi
S
Kume
D000818:Animals; D000831:Animals, Newborn / Q000097:blood / Q000378:metabolism*; D064026:Calbindins; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism; D003375:Coumestrol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D005260:Female; D007421:Intestine, Small / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007774:Lactation / Q000187:drug effects*; D008297:Male; D008431:Maternal-Fetal Exchange / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D048789:Phytoestrogens / Q000494:pharmacology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000235:genetics / Q000378:metabolism; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
D064026:Calbindins; D015220:Calcium Channels; D047628:Estrogen Receptor alpha; D048789:Phytoestrogens; D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D064030:S100 Calcium Binding Protein G; D050916:TRPV Cation Channels; C492240:Trpv5 protein, mouse; C446923:Trpv6 protein, mouse; D002118:Calcium; D003375:Coumestrol