Fluorescence in situ hybridization for cancer-related studies.
Cytogenetic analysis of tumour material has been greatly enhanced over the past 30 years by the application of a range of techniques based around fluorescence in situ hybridization (FISH). Fluorescence detection for in situ hybridization has the advantage of including the use of a multitude of fluorochromes to allow simultaneous specific detection of multiple probes by virtue of their differential labelling and emission spectra. FISH can be used to detect structural (translocation/inversion) and numerical (deletion/gain) genetic aberrations. This chapter will deal with FISH methods to detect and localize one or more complementary nucleic acid sequences (probes) within a range of different cellular targets including metaphase chromosomes, nuclei from cell suspension, and formalin-fixed paraffin-embedded FFPE tissue sections. Methods for the efficient localization of probes to FFPE tissue cores in tissue microarrays (TMAs) are also described.
Haemato-Oncology Research Unit, Division of Molecular Pathology, The Institute of Cancer Research, Surrey, UK.
Lyndal
L
Kearney
Janet
J
Shipley
D001710:Biotin / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002877:Chromosomes, Human / Q000235:genetics; D015342:DNA Probes / Q000378:metabolism; D004076:Digoxigenin / Q000378:metabolism; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase / Q000235:genetics; D008677:Metaphase / Q000235:genetics; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*; D016612:Paraffin Embedding; D046888:Tissue Array Analysis; D016707:Tissue Fixation
D015342:DNA Probes; D005819:Genetic Markers; D001710:Biotin; D004076:Digoxigenin