A highly selective G-quadruplex-based luminescent switch-on probe for the detection of gene deletion.
A G-quadruplex-based switch-on luminescence assay has been developed for the detection of gene deletion using a cyclometallated iridium(III) complex as a G-quadruplex-selective probe. Upon hybridization with the target DNA, the two split G-quadruplex-forming sequences assemble into a split G-quadruplex, which greatly enhances the luminescence emission of the iridium(III) probe. The assay is simple and highly selective.
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
Hong-Zhang
HZ
He
Daniel Shiu-Hin
DS
Chan
Chung-Hang
CH
Leung
Dik-Lung
DL
Ma
D056831:Coordination Complexes / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry; D054856:G-Quadruplexes; D017353:Gene Deletion; D007495:Iridium / Q000737:chemistry; D008163:Luminescent Measurements; D019713:Receptors, CCR5 / Q000235:genetics; D013056:Spectrophotometry, Ultraviolet
D056831:Coordination Complexes; D005456:Fluorescent Dyes; D019713:Receptors, CCR5; D007495:Iridium; D004247:DNA