Temporal change in IL-6 mRNA and protein expression produced by cyclic             stretching of human pulmonary artery endothelial cells.
The time courses of interleukin (IL)-6 gene expression and protein production were examined in human pulmonary artery endothelial cells (HPAECs) subjected to cyclic stretching. IL-6 protein was increased even in cells without stretching. Fold changes determined by dividing the level of IL-6 protein in stretched cells by that in unstretched cells at the same sampling times indicated that IL-6 protein was increased by stretching. At least 1 h of stretching was necessary to elicit an increase of IL-6 protein, and the levels peaked at 3 h after the start of stretching. After withdrawal of stretching, there was no further increase of IL-6 protein. The expression levels of the IL-6 gene were significantly increased by stretching and peaked at 30 min after the start of stretching. The difference in the peak times of IL-6 gene and protein expression likely reflects the process of protein synthesis after the appearance of IL-6 mRNA.
Graduate School of Medical Science, Kitasato University, Sagamihara, Minami-ku, Kanagawa 252-0373, Japan. kozukoba@kitasato-u.ac.jp
Kozue
K
Kobayashi
Masumi
M
Tanaka
Satoru
S
Nebuya
Kenichi
K
Kokubo
Yutaka
Y
Fukuoka
Yoshiteru
Y
Harada
Hirouske
H
Kobayashi
Makoto
M
Noshiro
Hidenori
H
Inaoka
D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism*; D011651:Pulmonary Artery / Q000378:metabolism*; D013314:Stress, Mechanical; D013997:Time Factors
D015850:Interleukin-6