Efficient quantification of HIV-1 in heparin plasma spiked with cultured HIV-1 by the Roche Cobas TaqMan and Abbott RealTime HIV-1 tests.
The current automated real-time HIV-1 viral load assays, the Roche Cobas AmpliPrep/Cobas TaqMan test and the Abbott RealTime test, are FDA cleared for use with EDTA plasma. We show that both real-time reverse transcription-PCR (RT-PCR) tests reliably quantify HIV-1 RNA in heparin plasma specimens spiked with HIV-1 isolate MN.
US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA. ljagodzinski@hivresearch.org
Linda L
LL
Jagodzinski
Holly R
HR
Weston
Ying
Y
Liu
Robert J
RJ
O'Connell
Sheila A
SA
Peel
D000925:Anticoagulants / Q000378:metabolism; D001331:Automation / Q000379:methods; D015497:HIV-1 / Q000302:isolation & purification*; D006493:Heparin / Q000378:metabolism; D006801:Humans; D010949:Plasma / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D019562:Viral Load / Q000379:methods*
D000925:Anticoagulants; D006493:Heparin