Apple pectin affects the efficacy of epigallocatechin gallate on oral sucrose tolerance test in adult mice.
Epigallocatechin gallate (EGCg), a dietary polyphenol and a major tea catechin, is a known sucrase inhibitor. Since dietary pectin is known to modulate some of the functions of the gastrointestinal tract, we investigated whether it could specifically affect the efficacy of EGCg on an oral sucrose tolerance test in mice. Male Crj:CD-1 (ICR) mice (seven weeks old) were randomly divided into two groups and fed a 5 % apple pectin (PE) or 5 % cellulose (CE) diet (control diet) for 28 days. After the experimental diet period, all mice were fasted overnight. A volume of 0.2 mL EGCg (20 mg/mL) was orally administered to all the mice by stainless steel feeding needle via injection syringe and a sucrose tolerance test was performed. The blood glucose levels were measured in blood collected from the tail vein using the OneTouch® Ultra® blood glucose monitoring system. Blood glucose levels at 30 minutes and 60 minutes after sucrose loading in the PE group were significantly higher than initial blood glucose levels. However, blood glucose levels at 30 minutes, 60 minutes, and 120 minutes after sucrose loading in the CE group were not significantly higher than initial blood glucose levels. After laparotomy, plasma lipids were also measured. Plasma triglyceride concentrations were significantly greater in the PE group than in the CE (control) group. This demonstrates that dietary pectin can affect the efficacy of EGCg on the oral sucrose tolerance test in mice.
National Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan. motoita@affrc.go.jp
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Tamura
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Hori
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002482:Cellulose / Q000494:pharmacology; D004043:Dietary Fiber / Q000494:pharmacology; D008055:Lipids / Q000097:blood; D008297:Male; D027845:Malus; D051379:Mice; D008813:Mice, Inbred ICR; D010368:Pectins / Q000494:pharmacology*; D013395:Sucrose
D001786:Blood Glucose; D004043:Dietary Fiber; D008055:Lipids; D013395:Sucrose; D010368:Pectins; D002392:Catechin; D002482:Cellulose; C045651:epigallocatechin gallate