Anti-cytomegalovirus effects of tricin are dependent on CXCL11.
It has been reported that treatment with tricin (4',5,7-trihydroxy-3',5'-dimethoxyflavone), a derivative of Sasa albo-marginata, after human cytomegalovirus (HCMV) infection significantly suppressed both infectious virus production and HCMV replication in the human embryonic fibroblast cell line MRC-5. In this paper, we examined the mechanisms for the anti-HCMV effects of tricin in MRC-5 cells. Exposure of fibroblasts to tricin inhibited infectious HCMV production, with concomitant decreases in levels of transcripts of the CXC chemokine IFN-inducible T cell alpha chemoattractant (I-TAC or CXCL11) gene. We also found that the transcripts of the HCMV immediate early (IE) gene and replication of HCMV were lower in CXCL11 gene-knockdown cells. These results suggest that tricin is a novel compound with potential anti-HCMV activity and that CXCL11 is one of the chemokines involved in HCMV replication. In addition, it is possible that CXCL11 is the one of the targets of tricin.
Department of Microbiology and Immunology, Hokuriku University, Ho-3 Kanagawa-machi, Kanazawa 920-1181, Japan. t-murayama@hokuriku-u.ac.jp
Tsugiya
T
Murayama
Ying
Y
Li
Takashi
T
Takahashi
Rie
R
Yamada
Keiko
K
Matsubara
Yuuzo
Y
Tuchida
Zhuan
Z
Li
Hidetaka
H
Sadanari
D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D054371:Chemokine CXCL11 / Q000235:genetics / Q000378:metabolism*; D003587:Cytomegalovirus / Q000187:drug effects* / Q000502:physiology; D005347:Fibroblasts / Q000187:drug effects / Q000821:virology; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D031787:Sasa / Q000737:chemistry; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; C515202:CXCL11 protein, human; D054371:Chemokine CXCL11; D005419:Flavonoids; D010936:Plant Extracts; C017769:tricin