[A new DNA methyltransferase M.BstC8I forms 5'-G(M5C)NNGC-3'. Studying of restriction endonuclease sensitivity to M.BstC8I-methylation].
Bacillus stearothermophilus C8 was grown up on the Luria agar at 37 degrees C. A new DNA-methylase was determined in cellular lysate. The methylation of the DNAs of bacteriophages lambda and T7 in the region of 5'-G(m5C)NNGC-3' blocked the activity of BstC8I. Specificity of M.BstC8I was analyzed on methylated lambda DNA. For this purpose, we used computer modeling and the data on the sensitivity of restrictases BstC8I, BsuRI, AjnI, and PvuII to methylation. The sensitivity of some restrictases to new methylation was studied. The results may be used for DNA methylation studying.
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Dedkov
D017123:Bacteriophage T7 / Q000737:chemistry; D010582:Bacteriophage lambda / Q000737:chemistry; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000737:chemistry* / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes / Q000737:chemistry*; D004795:Enzyme Stability; D001411:Geobacillus stearothermophilus / Q000737:chemistry*; D015183:Restriction Mapping
D015254:DNA Modification Methylases; C574045:DNA methyltransferase M.BstC8I, Bacillus stearothermophilus; D004262:DNA Restriction Enzymes