Functional differentiation in the leucine-rich repeat domains of closely related plant virus-resistance proteins that recognize common avr proteins.
The N' gene of Nicotiana sylvestris and L genes of Capsicum plants confer the resistance response accompanying the hypersensitive response (HR) elicited by tobamovirus coat proteins (CP) but with different viral specificities. Here, we report the identification of the N' gene. We amplified and cloned an N' candidate using polymerase chain reaction primers designed from L gene sequences. The N' candidate gene was a single 4143 base pairs fragment encoding a coiled-coil nucleotide-binding leucine-rich repeat (LRR)-type resistance protein of 1,380 amino acids. The candidate gene induced the HR in response to the coexpression of tobamovirus CP with the identical specificity as reported for N'. Analysis of N'-containing and tobamovirus-susceptible N. tabacum accessions supported the hypothesis that the candidate is the N' gene itself. Chimera analysis between N' and L(3) revealed that their LRR domains determine the spectrum of their tobamovirus CP recognition. Deletion and mutation analyses of N' and L(3) revealed that the conserved sequences in their C-terminal regions have important roles but contribute differentially to the recognition of common avirulence proteins. The results collectively suggest that Nicotiana N' and Capsicum L genes, which most likely evolved from a common ancestor, differentiated in their recognition specificity through changes in the structural requirements for LRR function.
Iwate Biotechnology Research Center, Iwate, Japan.
Ken-Taro
KT
Sekine
Reiko
R
Tomita
Shigeharu
S
Takeuchi
Go
G
Atsumi
Hiromasa
H
Saitoh
Hiroyuki
H
Mizumoto
Akinori
A
Kiba
Naoto
N
Yamaoka
Masamichi
M
Nishiguchi
Yasufumi
Y
Hikichi
Kappei
K
Kobayashi
D020816:Amino Acid Motifs; D002212:Capsicum / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D010935:Plant Diseases / Q000276:immunology / Q000821:virology; D018515:Plant Leaves; D010942:Plant Viruses / Q000276:immunology*; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D014026:Nicotiana / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014764:Viral Proteins / Q000276:immunology*
D014764:Viral Proteins