The use of fluorescent proteins for intravital imaging of cancer cell invasion.
The analysis of cancer cell behavior in the primary tumor in living animals provides an opportunity to explore the process of invasion and intravasation in the complex microenvironment that is present in vivo. In this chapter, we describe the methods that we have developed for performing intravital imaging of mammary tumors. We provide procedures for generating tumors through injection of tumor cell lines, and multiphoton imaging using a skin-flap tumor dissection and a mammary imaging window.
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, USA.
James
J
Hulit
Dmitriy
D
Kedrin
Bojana
B
Gligorijevic
David
D
Entenberg
Jeffrey
J
Wyckoff
John
J
Condeelis
Jeffrey E
JE
Segall
D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000473:pathology; D051379:Mice; D016513:Mice, SCID; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D051381:Rats