β-Amyrin oxidation by oat CYP51H10 expressed heterologously in yeast cells: the first example of CYP51-dependent metabolism other than the 14-demethylation of sterol precursors.
CYP51 has been recognized as a unique CYP family that consists of one isolated molecular species, a sterol 14-demethylase essential for sterol biosynthesis. However, another CYP51 gene classified as the CYP51H subfamily has been identified in higher plants, in addition to a sterol 14-demethylase gene, CYP51G1. To shed light on the function of this "second CYP51", oat CYP51H10 was introduced into the β-amyrin-producing yeast cells, and the effect of the expressed CYP51H10 on β-amyrin metabolism in the host cells was examined. In the CYP51H10-introduced cells, β-amyrin was converted to a metabolite with 12,13-epoxy and one additional hydroxyl group. Since the 12,13-epoxy group introduced into β-amyrin ring is an essential structure of avenacin A-1, a triterpene glycoside produced in oat from β-amyrin, the present findings indicate the contribution of CYP51H10 to avenacin A-1 biosynthesis from β-amyrin. This is the first study showing a second function of the CYP51 family.
Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo, Japan.
Mieko
M
Kunii
Yutaka
Y
Kitahama
Ery Odette
EO
Fukushima
Hikaru
H
Seki
Toshiya
T
Muranaka
Yuzo
Y
Yoshida
Yuri
Y
Aoyama
D018554:Avena / Q000201:enzymology / Q000235:genetics*; D017343:Genes, Plant; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D010840:Phytosterols / Q000096:biosynthesis; D012503:Saponins / Q000096:biosynthesis*; D058886:Sterol 14-Demethylase / Q000235:genetics* / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D010840:Phytosterols; D012503:Saponins; D009828:Oleanolic Acid; C091460:avenacin A 1; D058886:Sterol 14-Demethylase; C036380:beta-amyrin