A facile method to synthesize histones with posttranslational modification mimics.
Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.
Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
Zhiyong U
ZU
Wang
Yane-Shih
YS
Wang
Pei-Jing
PJ
Pai
William K
WK
Russell
David H
DH
Russell
Wenshe R
WR
Liu
D000107:Acetylation; D006657:Histones / Q000138:chemical synthesis* / Q000737:chemistry*; D047468:Immunoprecipitation; D008239:Lysine / Q000737:chemistry; D008745:Methylation; D015394:Molecular Structure; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D012694:Serine / Q000737:chemistry
D006657:Histones; D012694:Serine; D008239:Lysine