Anti-inflammatory activity of Bambusae Caulis in Taeniam through heme oxygenase-1 expression via Nrf-2 and p38 MAPK signaling in macrophages.
Recently, it has been reported that several natural extracts have anti-inflammatory effects through HO-1 induction. In this study, we used the ethyl acetate fraction of Bambusae Caulis in Taeniam (BCE) to investigate its anti-inflammatory effect on macrophages stimulated with LPS from Porphyromonas gingivalis. BCE inhibited the production of pro-inflammatory cytokines in P. gingivalis LPS-stimulated RAW264.7 cells. BCE also suppressed the nuclear translocation of NF-κB and AP-1. A selective inhibitor of HO-1 attenuated BCE's inhibitory effects on the production of pro-inflammatory cytokines. BCE also dose-dependently increased HO-1 expression at both the mRNA and the protein levels. BCE increased nuclear translocation of Nrf-2. Finally, a specific inhibitor of p38 reduced BCE-induced HO-1 expression and BCE-induced phosphorylation of p38 MAPK. BCE induced anti-inflammatory effects by activating Nrf-2-mediated HO-1 induction via p38 signaling in P. gingivalis LPS-stimulated macrophages. This result indicates that BCE is a promising therapeutic agent for combating periodontitis.
Department of Microbiology, Pusan National University, Busan 609-735, Republic of Korea.
Gwi Hae
GH
Jin
Bio-IT Fusion Technology Research Institute, Busan, Republic of Korea.
Sun Young
SY
Park
Department of Quality Assurance, Qsstem Implant, Busan, Republic of Korea.
Eunkyoung
E
Kim
Bio-IT Fusion Technology Research Institute, Busan, Republic of Korea.
Eun Yeon
EY
Ryu
Bio-IT Fusion Technology Research Institute, Busan, Republic of Korea.
Young Hun
YH
Kim
Institute for Research & Industry Cooperation, Busan, Republic of Korea.
Geuntae
G
Park
Department of Microbiology, Pusan National University, Busan 609-735, Republic of Korea. Electronic address: sangjoon@pusan.ac.kr.
Sang Joon
SJ
Lee
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems; D006109:Poaceae; D016966:Porphyromonas gingivalis; D015398:Signal Transduction / Q000187:drug effects; D018808:Transcription Factor AP-1 / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495636:Nfe2l2 protein, mouse; D010936:Plant Extracts; D018808:Transcription Factor AP-1; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D048051:p38 Mitogen-Activated Protein Kinases