Kidney proteome responses in the teleost fish Paralichthys olivaceus indicate a putative immune response against Streptococcus parauberis.
The proteomic response to bacterial infection in a teleost fish (Paralichthys olivaceus) infected with Streptococcus parauberis was analyzed using label-free protein quantitation coupled with LC-MS(E) tandem mass spectrometry. A total of 82 proteins from whole kidney, a major lymphoid organ in this fish, were found to be differentially expressed between healthy and diseased fish analyzed 6, 24, 72 and 120 h post-infection. Among the differentially expressed proteins, those involved in mediating immune responses (e.g., heat shock proteins, cathepsins, goose-type lysozyme and complement components) were most significantly up-regulated by infection. In addition, cell division cycle 48 (CDC48) and calreticulin, which are associated with cellular recovery and glycoprotein synthesis, were up-regulated in the universal protein group, whereas the other proteins in that group were down-regulated. There was continuous activation of expression of immune-associated proteins during infection, but there was also loss of expression of proteins not involved in immune function. We expect that our findings regarding immune response at the protein level would offer new insight into the systemic response to bacterial infection of a major immune organ in teleost fish.
Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea.
In-Seok
IS
Cha
Joseph
J
Kwon
Seong-Hwa
SH
Park
Seong-Won
SW
Nho
Ho-Bin
HB
Jang
Seong-Bin
SB
Park
Carmelo S
CS
del Castillo
Jun-ichi
J
Hikima
Takashi
T
Aoki
Tae-Sung
TS
Jung
D056704:Adaptive Immunity / Q000502:physiology; D000818:Animals; D016000:Cluster Analysis; D005393:Fish Diseases / Q000276:immunology / Q000378:metabolism*; D005432:Flounder / Q000276:immunology / Q000378:metabolism*; D007107:Immune System / Q000737:chemistry / Q000378:metabolism; D007668:Kidney / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008954:Models, Biological; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D013290:Streptococcal Infections / Q000276:immunology / Q000378:metabolism*; D013291:Streptococcus / Q000276:immunology*; D014018:Tissue Distribution
D020543:Proteome