Isolation and analysis of small RNAs from virus-infected plants.
In this chapter, we detail some of the methods available to the researcher for isolating and analyzing virus-derived small RNAs (vsRNAs). These methods have been successfully used for four plant viruses: Cucumber mosaic virus (CMV), including the CMV Y-Satellite, Turnip mosaic virus (TuMV), Potato leaf roll virus (PLRV), and Tomato spotted wilt virus (TSWV) from inoculated Arabidopsis thaliana plants (Fusaro et al. EMBO Rep 7:1168-1175, 2006; Curtin et al. FEBS Lett 582:2753-2760, 2008). The protocols presented here can also be employed for the isolation of non-virus related small RNAs such as microRNAs (miRNAs) and hairpin RNA (hpRNA).
Department of Agronomy and Plant Genetics, University of Minnesota, Minnesota, MN, USA.
Shaun J
SJ
Curtin
Agustín
A
Zsögön
John M
JM
Watson
Peter M
PM
Waterhouse
D017360:Arabidopsis / Q000235:genetics / Q000821:virology*; D017799:Cucumovirus / Q000235:genetics; D052679:Luteoviridae / Q000235:genetics; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics*; D034741:RNA, Small Interfering / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D017865:Tospovirus / Q000235:genetics; D017806:Tymovirus / Q000235:genetics
D035683:MicroRNAs; D034741:RNA, Small Interfering; D012367:RNA, Viral