Metabolite profiles of rice cultivars containing bacterial blight-resistant genes are distinctive from susceptible rice.
The metabolic changes of bacterial blight-resistant line C418/Xa23 generated by molecular marker-assisted selection (n= 12), transgenic variety C418-Xa21 generated by using the Agrobacterium-mediated system (n= 12), and progenitor cultivar C418 (n= 12) were monitored using gas chromatography/mass spectrometry. The validation, discrimination, and establishment of correlative relationships between metabolite signals were performed by cluster analysis, principal component analysis, and partial least squares-discriminant analysis. Significant and unintended changes were observed in 154 components in C418/Xa23 and 48 components in C418-Xa21 compared with C418 (P< 0.05, Fold change > 2.0). The most significant decreases detected (P< 0.001) in both C418/Xa23 and C418-Xa21 were in three amino acids: glycine, tyrosine, and alanine, and four identified metabolites: malic acid, ferulic acid, succinic acid, and glycerol. Linoleic acid was increased specifically in C418/Xa23 which was derived from traditional breeding. This line, possessing a distinctive metabolite profile as a positive control, shows more differences vs. the parental than the transgenic line. Only succinic acid that falls outside the boundaries of natural variability between the two non-transgenic varieties C418 and C418/Xa23 should be further investigated with respect to safety or nutritional impact.
Institute of Tropic Bioscience and Biotechnology,  Chinese Academy of Tropic Agricultural Sciences, Haikou 571101,  China.
Jiao
J
Wu
Haichuan
H
Yu
Haofu
H
Dai
Wenli
W
Mei
Xin
X
Huang
Shuifang
S
Zhu
Ming
M
Peng
D000409:Alanine / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D003373:Coumaric Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005990:Glycerol / Q000378:metabolism; D016018:Least-Squares Analysis; D019787:Linoleic Acid / Q000737:chemistry; D008293:Malates / Q000378:metabolism; D055432:Metabolomics / Q000379:methods; D008957:Models, Genetic; D012275:Oryza / Q000235:genetics* / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D025341:Principal Component Analysis; D019802:Succinic Acid / Q000378:metabolism; D019076:Transgenes; D014443:Tyrosine / Q000378:metabolism
D003373:Coumaric Acids; D008293:Malates; D010940:Plant Proteins; D014443:Tyrosine; C030298:malic acid; D019787:Linoleic Acid; D019802:Succinic Acid; C004999:ferulic acid; D000409:Alanine; D005990:Glycerol