Visual detection of DNA mutation using multicolor fluorescent coding.
A visual colorimetric method for detecting multiplex DNA mutations has been developed using multicolor fluorescent coding based on multistep FRET of cationic conjugated polymers. Expensive instruments and technical expertise are not required in this method. Also our visual system provides a quantitative detection by simply analyzing RGB values of images. Genomic DNAs extracted from 60 FFPE colon tissues can be sensitively determined by utilizing our visual assay with a high-throughput manner. Thus, it proves to be sensitive, reliable, cost-effective, simple, and high-throughput for mutation detection.
Beijing National Laboratory for Molecular Science, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences , Beijing, 100190, P. R. China.
Jinzhao
J
Song
Qiong
Q
Yang
Fengting
F
Lv
Libing
L
Liu
Shu
S
Wang
D045744:Cell Line, Tumor; D058534:Class I Phosphatidylinositol 3-Kinases; D004247:DNA / Q000032:analysis*; D005449:Fluorenes / Q000737:chemistry; D019793:Fluorescein / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D009154:Mutation; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
D005449:Fluorenes; D005456:Fluorescent Dyes; D011108:Polymers; D000644:Quaternary Ammonium Compounds; C526234:poly(9,9-bis(6'-N,N,N-trimethylammonium)hexyl)fluorenephenylene bromide; D004247:DNA; D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human; D019793:Fluorescein