A standardized extract of Ginkgo biloba neutralizes cisplatin-mediated reproductive toxicity in rats.
The aim of this study was to evaluate the protective effects of Ginkgo biloba (GB) against testicular damage and oxidative stress as well as caudal sperm indices in a cisplatin- (CIS-) induced rodent model. Adult male Wistar rats were given vehicle, single i.p. dose of CIS alone (10 mg/kg), GB alone (200 mg g/kg every day for five days), or single dose of CIS followed by GB (50, 100, or 200 mg/kg every day for five days). On day 6, after the first drug treatment oxidative and apoptotic testicular toxicity was evaluated. CIS-treated rats displayed decreased weights of testes and epididymis as well as caudal sperm count and motility. This reproductive toxicity was accompanied with increased germ-cell degeneration in seminiferous tubules and increased germ-cell apoptosis, increased testicular MDA levels and MPO activity, and decreased SOD and CAT activities in testes. Intensive expressions of COX-2, iNOS, and NF-κB p65 in testicular tissues were detected in CIS-treated group. Oral GB administrations at all doses to CIS-treated rats effectively alleviated all of the CIS-induced toxicity in reproductive system. The present results provide further insights into the mechanisms of protection against CIS-induced reproductive toxicity and confirm the essential antioxidant potential of a GB extract.
Biology Department, United Arab Emirates University, Al Ain 17551, UAE. a.amin@uaeu.ac.ae
Amr
A
Amin
Christeena
C
Abraham
Alaaeldin A
AA
Hamza
Zeinab A
ZA
Abdalla
Shaikha B
SB
Al-Shamsi
Saina S
SS
Harethi
Sayel
S
Daoud
D000704:Analysis of Variance; D000818:Animals; D002945:Cisplatin / Q000633:toxicity*; D004347:Drug Interactions; D004822:Epididymis / Q000187:drug effects; D020441:Ginkgo biloba / Q000737:chemistry*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013081:Sperm Motility / Q000187:drug effects; D013737:Testis / Q000187:drug effects* / Q000473:pathology*
D010936:Plant Extracts; D020011:Protective Agents; D002945:Cisplatin