Experience-dependent brain plasticity after stroke: effect of ibuprofen and poststroke delay.
Despite indications that brain plasticity may be enhanced after stroke, we have described impairment of experience-dependent plasticity in rat cerebral cortex neighboring the stroke-induced lesion. Photothrombotic stroke was centered behind the barrel cortex in one cerebral hemisphere of rats. Plasticity of cortical representation of one row of vibrissae was induced by sensory deprivation of all surrounding whiskers for 1 month, and visualized with [(14)C]-2-deoxyglucose autoradiography. In control rats deprivation resulted in an enlargement of functional cortical representation of the spared row of vibrissae. After a focal stroke neighbouring the barrel cortex, no plasticity of the spared row representation was found. Investigation of plastic changes with deprivation initiated 1 week and 1 month after stroke have shown that later poststroke onset of deprivation resulted in a partial recovery of cortical plasticity in the barrel field. Western blot analysis of proinflammatory enzyme cyclooxygenase-2 (COX-2) expression revealed its strong upregulation in the barrel cortex 24 h after stroke. When chronic treatment with the anti-inflammatory drug ibuprofen (10 mg/kg or 20 mg/kg) accompanied deprivation, plasticity was restored. Ibuprofen applied before the ischemia also prevented the poststroke upregulation of COX-2. The results strongly suggest that poststroke impairment of experience-dependent cortical plasticity is caused by stroke-induced inflammatory reactions that subside with poststroke delay and can be at least partially ameliorated by pharmacological treatment.
Department of Animal Physiology, Faculty of Biology, Warsaw University, Warsaw, Poland.
Jan A
JA
Jablonka
Malgorzata
M
Kossut
Otto W
OW
Witte
Monika
M
Liguz-Lecznar
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D007052:Ibuprofen / Q000494:pharmacology*; D008297:Male; D009473:Neuronal Plasticity / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012683:Sensory Deprivation; D020521:Stroke / Q000503:physiopathology*; D015854:Up-Regulation
D000894:Anti-Inflammatory Agents, Non-Steroidal; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D007052:Ibuprofen