Aurora kinase B expression in breast carcinoma: cell kinetic and genetic aspects.
BACKGROUND
Mitotic deregulations may contribute significantly to cell division errors and the development of aggressive tumor cells. The mitotic kinase Aurora B is essential for chromosome segregation. Its gene is located at 17p13 in close proximity to the TP53 gene. Although the frequent alteration of this locus is well known, the information about the AURKB status and protein expression is limited.


METHODS
50 breast carcinoma cases were evaluated for 17p13 status and chromosome 17 ploidy by FISH and for Aurora B protein by immunohistochemistry.


RESULTS
Aurora B protein expression showed a strong correlation with cell proliferation (regression coefficient = 0.77). Therefore, the Aurora B/MIB-1 index was used as a measure of expression, which showed a wide range (1-35%, mean 0.32, SD ± 0.28). A gain in the 17p13 chromosome locus could not be shown while a deletion was stated in 10/50 cases including a subset with TP53 and AURKB codeletion in 6/10 cases. The loss of TP53/AURKB loci strongly correlated with aneusomy (p < 0.0001).


CONCLUSIONS
Elevated Aurora B expression frequently occurs due to an increased cell proliferation rate in breast carcinoma. Codeletion of TP53 and AURKB at 17p13 indicates a concerted mechanism leading to the survival of cell clones with deficient mitotic kinase function which could contribute to the formation of aneuploid cells and an aggressive tumor phenotype.
Department of Pathology, University of Debrecen Medical and Health Science Center, Debrecen, Hungary.
Katalin
K
Hegyi
Kristóf
K
Egervári
Zsuzsa
Z
Sándor
Gábor
G
Méhes
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D064107:Aurora Kinase B; D064127:Aurora Kinases; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000201:enzymology / Q000235:genetics*; D018275:Carcinoma, Lobular / Q000201:enzymology / Q000235:genetics*; D002453:Cell Cycle; D049109:Cell Proliferation; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D056915:DNA Copy Number Variations; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008875:Middle Aged; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism
D019394:Ki-67 Antigen; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases