Production of mycosporine-like amino acids of in situ phytoplankton community in Kongsfjorden, Svalbard, Arctic.
The spatial distribution of UV-absorbing compounds (mycosporine-like amino acids, MAAs), was investigated by comparing the phytoplankton community structures in the inner and outer waters of the Kongsfjorden inlet, which is located in arctic Svalbard. Thalassiosira sp. and Phaeocystis sp. were dominant in the outer waters of the Kongfjorden inlet, demonstrating high chlorophyll a (chl a) concentrations and low MAA concentrations in the outer bay waters. However, Kongsfjorden Bay was dominated by Phaeocystis sp. and demonstrated high MAA concentrations despite low chl a concentrations. The carbon fixation rate at a station located inside Kongsfjorden Bay (T05) was significantly photo-inhibited by UV radiation, demonstrating higher production rates of MAA and chl a than at a station (B09) in outer bloom waters. Additionally, the turnover rates of individual MAAs were faster inside the Kongsfjorden Bay than in the outside waters. As a result, the natural phytoplankton community demonstrated different UV adaptation mechanisms according to the phytoplankton species, in this case, Thalassiosira sp. vs. Phaeocystis sp. It is possible to understand real-time changes for newly photosynthesized MAAs as UV-absorbing compounds in the natural phytoplankton community. This takes place via determination of in situ MAA production rates using (13)C tracer and High Performance Liquid Chromatography (HPLC) combined with an isotope ratio mass spectrometer (irMS).
Hanyang University, Department of Marine Environmental Science, 1271-3 dong, Sangnok-gu, Ansan, Kyeonggi-do 425-791, South Korea.
Sun-Yong
SY
Ha
Young-Nam
YN
Kim
Mi-Ok
MO
Park
Sung-Ho
SH
Kang
Hyun-choel
HC
Kim
Kyung-Hoon
KH
Shin
D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D002247:Carbon Isotopes / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism; D000077194:Chlorophyll A; D002851:Chromatography, High Pressure Liquid; D003511:Cyclohexanols / Q000737:chemistry*; D058087:Haptophyta / Q000378:metabolism; D013058:Mass Spectrometry; D010788:Photosynthesis; D010839:Phytoplankton / Q000378:metabolism*; D013538:Svalbard; D014466:Ultraviolet Rays
D000596:Amino Acids; D002247:Carbon Isotopes; D003511:Cyclohexanols; D002734:Chlorophyll; D000077194:Chlorophyll A