[Attenuation of experimental peritonitis and periodontitis in human chemokine-like receptor 1 (CMKLR1 or hChemR23) transgenic mice].
OBJECTIVE
To prepare a transgenic mouse model with myeloid-selective expression of human chemokine-like receptor 1 (CMKLR1 or hChemR23)  and investigate the in vivo inflammatory response of the particular transgenic mice in induced-peritonitis and periodontitis.


METHODS
The full-length  hChemR23 cDNA and hCD11b promoters were cloned into pcDNA3 plasmid. Purified transgene (2.9 kb) was used to make transgenic mice at Boston  University Transgenic/Knock out Mouse Core Facility. Copy numbers and mRNA level of hChemR23 transgene in the founders were verified by Custom  TaqMan Gene Expression Real-time PCR assay. Hemizygous colonies were amplified by out-breeding hChemR23 transgenic males with wide-type  FVB females. Transgenic mice were injected intraperitoneally with 1 mL of zymosan A solution (1 g/L in PBS). Peritoneal lavage cells stained with PE-conjugated anti-mouse F4/80 and FITC-conjugated anti-mouse Ly6G were analyzed by FACSort. Then 9-0 silk ligature was tied around the first molar  to induce a periotontitis mouse model. The distance of cementoenamel junction to the alveolar bone crest (CEJ-ABC) was calculated to evaluate the  alveolar bone loss. Data were analyzed with student's t-test and variance analysis.


RESULTS
One out of the four F1 founders carried the hChemR23  transgene. hChemR23 transgenic mice had reduced 56% fewer positive labeled polymorphonuclear neutrophil (PMNs) in peritonitis abdominal lavage  fluid as compared with wide-type littermates. In a ligature-induced peirodontitis mouse model, hChemR23 transgenic mice showed decreased alveolar  bone loss as compared with WT littermates (P<0.05).


CONCLUSIONS
The hChemR23 transgenic mice over-expressed the hChemR23 at mRNA level  and had reduced inflammation in the acute peritonitis and ligature-induced periodontitis model.
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China. gaolily1979@yahoo.com.cn
Li
L
Gao
Robert
R
Cyurko
Thomas
T
Van Dyke
D016301:Alveolar Bone Loss / Q000473:pathology; D000818:Animals; D005260:Female; D007249:Inflammation / Q000276:immunology*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009504:Neutrophils; D010518:Periodontitis / Q000276:immunology*; D010538:Peritonitis / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019707:Receptors, Chemokine / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
C102741:CMKLR1 protein, human; D012333:RNA, Messenger; D019707:Receptors, Chemokine