Emergence of Salmonella enterica serovar infantis harboring IncI1 plasmid with bla(CTX-M-14) in a broiler farm in Japan.
Cefotaxime (CTX)-resistant and -susceptible Salmonella enterica serovar Infantis isolates obtained from broilers raised on a farm in January 2010 in Japan were characterized to establish their resistance determinants. The CTX-resistant isolates produced CTX-M-14 extended-spectrum β-lactamase and harbored 2 distinct plasmid of approximately 140- and 95-kb, whereas the CTX-susceptible isolates harbored one 140-kb plasmid. The 95-kb plasmids were replicon typed as IncI1 carrying the bla(CTX-M-14) gene, while the 140-kb plasmids were IncP and harbored the aphA1, aadA1, tetA, and sul1 genes. Genetic fingerprinting by pulsed-field gel electrophoresis revealed similar macrorestriction profiles amongst CTX-resistant and susceptible isolates, suggesting a clonal relationship. The presence of CTX-resistant S. Infantis on a broiler farm has occurred through the acquisition of IncI1 resistance plasmid.
Yamaguchi Prefectural Institute of Public Health and Environment, 2-5-67 Aoi, Yamaguchi 753-0821, Japan.
Mitsuhiro
M
Kameyama
Takehisa
T
Chuma
Tomoki
T
Yokoi
Junko
J
Yabata
Kiyoshi
K
Tominaga
Daisuke
D
Miyasako
Hiroyuki
H
Iwata
Karoku
K
Okamoto
D000818:Animals; D002645:Chickens; D021821:Communicable Diseases, Emerging / Q000453:epidemiology / Q000662:veterinary*; D016172:DNA Fingerprinting / Q000662:veterinary; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D007564:Japan / Q000453:epidemiology; D010957:Plasmids / Q000235:genetics; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000453:epidemiology*; D019779:Salmonella enterica / Q000235:genetics*; D001618:beta-Lactamases / Q000378:metabolism
D001618:beta-Lactamases