[Association of sterol regulatory element binding protein-1c genetic polymorphisms rs2297508 and rs11868035 with type 2 diabetes mellitus in Gansu Han and Dongxiang population].
OBJECTIVE
To assess the association between single nucleotide polymorphisms (SNP) rs2297508 and rs11868035 of sterol regulatory element binding protein-1c (SREBP-1c) gene and type 2 diabetes mellitus (T2DM) in Han and Dongxiang populations in Gansu.


METHODS
A total of 342 patients with T2DM and 343 healthy controls of Han ethnics and 218 patients with T2DM and 238 healthy controls of Dongxiang ethnics were enrolled during 2006 and 2009. Genotypes of rs2297508 and rs11868035 were determined by polymerase chain reaction-denaturing high performance liquid chromatography (PCR-DHPLC). Plasma levels of lipid, glucose and insulin in all subjects were assayed by biochemical techniques. Chi-square test was used for data analysis.


RESULTS
The genotypic and allelic frequencies of rs2297508 and rs11868035 polymorphisms did not differ significantly in the control groups of Han and Dongxiang ethnics (P> 0.05). But those of T2DM patients were significantly higher than in the controls (P< 0.01). Plasma low density lipoprotein cholesterol (LDL-C) levels for carriers of C allele for rs2297508 were significantly higher than those of non-carriers from the control group of Han ethnics (P<0.05). That of carriers of CC genotypes for rs11868035 was significantly higher than carriers of GG genotype in the control group of Dongxiang ethnics (P<0.05).


CONCLUSIONS
SNP rs2297508 and rs11868035 of SREBP-1c gene may be associated with T2DM, and C allele appears to be a risk factor for T2DM in Gansu Han or Dongxiang ethnics. Han and Dongxiang ethnics did not differ significantly in terms of genotypic and allelic frequencies for the two SNPs. SNP rs2297508 of SREBP-1c gene may be associated with increased plasma levels of LDL-C in both Han or Dongxiang ethnics.
Department of Endocrinology, People's Hospital of Gansu Province, Lanzhou, Gansu 730000, P. R. China.
Ju-xiang
JX
Liu
Jing
J
Liu
Qian
Q
Guo
Jia
J
Liu
D000483:Alleles; D044466:Asian People; D001483:Base Sequence; D001786:Blood Glucose; D008078:Cholesterol, LDL / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D051780:Sterol Regulatory Element Binding Protein 1 / Q000097:blood / Q000235:genetics*
D001786:Blood Glucose; D008078:Cholesterol, LDL; D007328:Insulin; D051780:Sterol Regulatory Element Binding Protein 1