Studying the generation of regenerated retinal neuron from Müller glia in the mouse eye.
Retinal regeneration has been studied for decades in nonmammalian species. From these studies, we learned that retinal Müller glia are a potential source of neuronal regeneration by de novo neurogenesis. Although spontaneous regeneration in mammals is absent after retinal damage, we discovered that certain manipulations stimulate a limited regenerative program in adult mice. This allows the study of cellular and molecular barriers that limit regeneration in mice and man as well as to compare it to regenerative species with more complete repair-like fish and birds. Thereby, it may offer novel means to the better understanding of retinal regeneration and develop successful therapies of retinal diseases. In this chapter, we describe the methods to study Müller glia-derived regeneration in adult mice in vivo and discuss potential caveats.
Department of Biological Structure, Institute for Stem Cells and Regenerative Medicine, University of Washington, Seattle, WA, USA.
Mike O
MO
Karl
Thomas A
TA
Reh
D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D012038:Regeneration; D012160:Retina / Q000187:drug effects / Q000293:injuries; D055351:Retinal Neurons / Q000378:metabolism / Q000502:physiology*