Escherichia coli exhibits a membrane-related response to a small arginine- and tryptophan-rich antimicrobial peptide.
Since multiresistant bacterial strains are more widespread and the victim numbers steadily increase, it is very important to possess a broad bandwidth of antimicrobial substances. Antibiotics often feature membrane-associated effect mechanisms. So, we present a membrane proteomic approach to shed light on the cellular response of Escherichia coli as model organism to the hexapeptide MP196, which is arginine and tryptophan rich. Analyzing integral membrane proteins are still challenging, although various detection strategies have been developed in the past. In particular, membrane proteomics in bacteria have been conducted very little due to the special physical properties of these membrane proteins. To obtain more information on the cellular response of the new compound group of small peptides, the tryptophan- and arginine-rich hexapeptide MP196 was subject to a comprehensive quantitative membrane proteomic study on E. coli by means of metabolic labeling in combination with membrane lipid analyses. This study provides in total 767 protein identifications including 185 integral membrane proteins, from which 624 could be quantified. Among these proteins, 134 were differentially expressed. Thereby, functional groups such as amino acid and membrane biosynthesis were affected, stress response could be observed, and the lipid composition of the membrane was significantly altered. Especially, the strong upregulation of the envelope stress induced protein. Spy indicates membrane damage, as well as the downregulation of the mechano-sensitive channel MscL beside others. Finally, the exceptional downregulation of transport systems strengthens these findings.
Lehrstuhl für Analytische Chemie, Biomolekulare Massenspektrometrie, Fakultät für Chemie und Biochemie, Ruhr-Universität Bochum, Bochum, Germany.
Benjamin
B
Fränzel
Maya
M
Penkova
Christian
C
Frese
Nils
N
Metzler-Nolte
Dirk
D
Andreas Wolters
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000032:analysis / Q000378:metabolism; D008563:Membrane Lipids / Q000032:analysis / Q000737:chemistry; D053858:Metabolic Networks and Pathways; D008826:Microbial Sensitivity Tests; D020543:Proteome / Q000187:drug effects / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D029968:Escherichia coli Proteins; D008563:Membrane Lipids; D020543:Proteome