Effects of an experimental calcium aluminosilicate cement on the viability of murine odontoblast-like cells.
BACKGROUND
Quick-setting calcium aluminosilicate cement with improved washout resistance is a potential substitute for calcium silicate cements in endodontics. This study examined the effect of an experimental calcium aluminosilicate cement (Quick-Set; Primus Consulting, Bradenton, FL) on the viability of odontoblast-like cells.


METHODS
The biocompatibility of Quick-Set and white ProRoot MTA (WMTA; Dentsply Tulsa Dental Specialties, Tulsa, OK) cements and their eluents was evaluated using a murine dental papilla-derived odontoblast-like cell line (MDPC-23); 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to examine the effects of the 2 hydraulic cements on mitochondrial metabolic activity. Flow cytometry and confocal laser scanning microscopy were used to identify the effects of the 2 cements on cell death-induced plasma membrane permeability to fluorescent dyes and DNA stains.


RESULTS
After the first week of immersion in culture medium, Quick-Set and WMTA were more cytotoxic than the Teflon-negative control (P < .05), and the cells exhibited more apoptosis/necrosis than Teflon (P < .05). After the second week of immersion, the 2 cements were as biocompatible as Teflon (P > .05), with cells exhibiting minimal apoptosis/necrosis. Eluents from the set cements at 1:1 dilution were significantly more cytotoxic that eluents at 1:10 or 1:100 dilution (P < .05).


CONCLUSIONS
Quick-Set and WMTA exhibited similar cytotoxicity profiles. They possess negligible in vitro toxicologic risks after time-dependent elution of toxic components.
Department of Stomatology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.
Wei
W
Wei
Yi-pin
YP
Qi
Sergey Y
SY
Nikonov
Li-na
LN
Niu
Regina L W
RL
Messer
Jing
J
Mao
Carolyn M
CM
Primus
David H
DH
Pashley
Franklin R
FR
Tay
D017607:Aluminum Compounds / Q000633:toxicity; D000538:Aluminum Silicates / Q000737:chemistry / Q000633:toxicity; D000818:Animals; D017610:Calcium Compounds / Q000737:chemistry / Q000633:toxicity; D016923:Cell Death; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D004338:Drug Combinations; D005434:Flow Cytometry; D008422:Materials Testing; D051379:Mice; D018613:Microscopy, Confocal; D009804:Odontoblasts / Q000187:drug effects*; D010087:Oxides / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012823:Silicate Cement / Q000633:toxicity*; D017640:Silicates / Q000737:chemistry / Q000633:toxicity
D017607:Aluminum Compounds; D000538:Aluminum Silicates; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D012823:Silicate Cement; C049037:aluminosilicate; C031293:calcium silicate