Oroxylum indicum seeds--analysis of flavonoids by HPLC-MS.
The seeds of Oroxylum indicum Vent. play an important role in Traditional Chinese Medicine to treat infectious diseases of the respiratory tract. In this manuscript the first HPLC-MS assay for the separation of all major flavonoids as well as their isolation and quantification is reported. The developed method was fully validated and showed to be accurate (recovery rates≥96.2%), precise (intra-day σ(rel)≤4.83%, inter-day σ(rel)≤5.23%), repeatable (σ(rel)≤2.57), sensitive (LOD: 0.05-0.34 μg/ml) and linear (R(2)≥0.999). Four samples of Oroxyli semen from different origins were separated with this optimized HPLC procedure on a Phenomenex Luna 5 μ C8 (2) 100 Å column using acetonitrile and water/0.1% formic acid as mobile phase. Temperature, flow rate and injection volume were set to 25 °C, 0.5 ml/min and 10 μl, respectively. The most dominant flavonoid showed to be Baicalein-7-O-β-D-gentiobiosid, occurring in the specimens in percentages ranging from 0.92 to 4.08%.
Institute of Pharmacy, Pharmacognosy, University of Innsbruck, Innrain 80-82, 6020 Innsbruck, Austria.
Anja
A
Krüger
Markus
M
Ganzera
D000097:Acetonitriles / Q000737:chemistry; D029402:Bignoniaceae / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D005419:Flavonoids / Q000032:analysis*; D005561:Formates / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000592:standards; D010946:Plants, Medicinal; D012015:Reference Standards; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013696:Temperature; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D005419:Flavonoids; D005561:Formates; D014867:Water; C030544:formic acid; C032159:acetonitrile