Effects of intercellular adhesion molecule-1 on renal damage in spontaneously hypertensive rats.
OBJECTIVE
Intercellular adhesion molecule-1 (ICAM-1) plays an important role in the inflammatory process and immune response. The aim of the study was to investigate ICAM-1 expression in the kidneys of spontaneously hypertensive rats (SHRs) and the relationship between the level of ICAM-1 and renal damage.


METHODS
Male Wistar-Kyoto (WKY) rat and SHR models were employed. Blood pressure (BP) was recorded using tail cuff method. Twenty-four hour proteinuria and β(2)-microglobulin (β(2)-MG) were measured using biuret method and radioimmunity kits, respectively. Biochemical parameters were measured after the animals were killed. ICAM-1 expression in renal tissue was assessed using western blot and real-time polymerase chain reaction (PCR).


RESULTS
It was observed that BP, proteinuria, and urine β(2)-MG were more increased in SHR groups than that in the same age WKY groups. In SHR groups, BP, proteinuria, and urine β(2)-MG at the 56th week were significantly higher than that at the 28th week. ICAM-1 protein and mRNA expression in SHR renal tissues was significantly increased in SHR groups compared with the same age in WKY rats. ICAM-l expression was positively correlated with proteinuria and urine β(2)-MG.


CONCLUSIONS
This study demonstrated that the renal expression of ICAM-1 was increased in SHR with renal damage, and inflammation may be involved in the hypertensive renal damage.
Department of Nephrology, The Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
Ding-Wu
DW
Zhang
Xin-Zhong
XZ
Huang
Jian-Hua
JH
Wu
Ya-Pin
YP
Fan
Hui
H
Shi
D000818:Animals; D062186:Arterial Pressure; D006973:Hypertension / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007668:Kidney / Q000473:pathology*; D008297:Male; D011507:Proteinuria / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D001613:beta 2-Microglobulin / Q000378:metabolism
D012333:RNA, Messenger; D001613:beta 2-Microglobulin; D018799:Intercellular Adhesion Molecule-1