Using the Neisseria gonorrhoeae multiantigen sequence-typing method to assess strain diversity and antibiotic resistance in San Francisco, California.
Urogenital Neisseria gonorrhoeae isolates (266) collected in San Francisco, CA, in 2009 were analyzed for antimicrobial susceptibility and were subsequently genotyped by N. gonorrhoeae multiantigen sequence typing (NG-MAST). Isolates of identical or closely related sequence types were found to possess highly similar phenotypes with regard to drug susceptibility. Isolates containing decreased susceptibility to oral cephalosporins were detected in 2009 and were found to contain the mosaic penA allele (XXXIV) found previously to be associated with decreased susceptibility to cephalosporins. A better understanding of the relationships between phenotypic and genotypic markers for antimicrobial resistance may be helpful to the development of effective surveillance systems for drug-resistant N. gonorrhoeae.
San Francisco Department of Public Health Laboratory, San Francisco, California 94102, USA.
Sean
S
Buono
Abel
A
Wu
David C
DC
Hess
Jonathan S
JS
Carlson
Leah
L
Rauch
Susan S
SS
Philip
Pennan
P
Barry
Kyle
K
Bernstein
Jeffrey D
JD
Klausner
Mark W
MW
Pandori
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques / Q000379:methods; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006069:Gonorrhea / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D010641:Phenotype; D012495:San Francisco / Q000453:epidemiology; D017422:Sequence Analysis, DNA
D000900:Anti-Bacterial Agents