In vivo functional analysis of transcription factor: response element interaction using transgenic Xenopus laevis.
Analysis of transcription factor-target interactions in vivo is important to the study of transcriptional regulation of gene expression. A key experiment involves analysis of the functional interaction between a trans-acting factor and its corresponding cis-acting element in the context of a target promoter in vivo. We describe a method for this analysis in transgenic Xenopus tadpoles in which expression of the trans-acting factor is knocked down using an shRNA-mediated approach.
Center for Molecular and Human Genetics, The Research Institute at Nationwide Children's Hospital, Columbus, OH, USA. Heithem.El-Hodiri@NationwideChildrens.org
Heithem M
HM
El-Hodiri
Yi
Y
Pan
Lisa E
LE
Kelly
D000818:Animals; D030801:Animals, Genetically Modified; D002467:Cell Nucleus; D005260:Female; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D018390:Gene Targeting; D008297:Male; D053652:Nuclear Transfer Techniques / Q000295:instrumentation; D034741:RNA, Small Interfering / Q000378:metabolism; D020218:Response Elements; D013094:Spermatozoa / Q000166:cytology; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014982:Xenopus laevis / Q000235:genetics* / Q000378:metabolism
D034741:RNA, Small Interfering; D015534:Trans-Activators; D014157:Transcription Factors