Multiple sclerosis-linked and interferon-beta-regulated gene expression in plasmacytoid dendritic cells.
The cause of multiple sclerosis (MS) is not known and the mechanism of interferon-beta, a disease-modifying treatment, is not well-understood. We studied gene expression in plasmacytoid dendritic cells (pDCs), antigen-presenting cells implicated in MS pathogenesis. PDCs were separated from healthy donors and MS patients at two time points: before and after initiation of treatment with interferon-beta. Expression of selected MS-linked and interferon-beta-regulated genes was validated with single assays. We have identified 60 genes which were abnormally expressed in MS patients and were corrected after treatment. These genes could be studied as potential MS biomarkers and possible therapeutic targets in MS.
Department of Neurology, Robert Wood Johnson Medical School, New Brunswick, NJ 08901, United States.
Latt Latt
LL
Aung
Andrew
A
Brooks
Steven A
SA
Greenberg
Michael L
ML
Rosenberg
Suhayl
S
Dhib-Jalbut
Konstantin E
KE
Balashov
D000328:Adult; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D006801:Humans; D016899:Interferon-beta / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008297:Male; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016899:Interferon-beta