Lacticin LC14, a new bacteriocin produced by Lactococcus lactis BMG6.14: isolation, purification and partial characterization.
A new bacteriocin, lacticin LC14, produced by Lactococcus lactis BMG6.14, was isolated and characterized. It was purified to homogeneity from overnight broth culture by ammonium sulfate precipitation, Sep-Pak chromatography, and two steps of reversed-phase HPLC. Lacticin LC14 showed bactericidal-type antimicrobial activity against several lactic acid bacteria and pathogenic strains including Listeria monocytogenes. It was inactivated by proteinase K and pronase E, but was resistant to papain, lysozyme, lipase and catalase. Lacticin LC14 was heat resistant, stable over a wide range of pH (2-10) and after treatment by solvents and detergents. Its N-terminal end was found unreactive towards Edman sequencing. Based on MALDI-TOF mass spectrometry, its molecular mass was 3333.7 Da. LC14 amino acid composition revealed a high proportion of hydrophobic residues, but no modified ones. LC14 may be able to challenge other well known other bacteriocins in probiotic and therapeutic applications.
Laboratoire de Biochimie, CNRS FRE 2738, Faculté de Médecine Nord, Bd Pierre Dramard, 13916 Marseille Cedex 20, France.
Samar
S
Lasta
Hadda
H
Ouzari
Nicolas
N
Andreotti
Ziad
Z
Fajloun
Pascal
P
Mansuelle
Abdellatif
A
Boudabous
Francois
F
Sampieri
Jean Marc
JM
Sabatier
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001430:Bacteriocins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013294:Lactococcus lactis / Q000302:isolation & purification / Q000378:metabolism*
D000596:Amino Acids; D001430:Bacteriocins