Targeting pre-miRNA by peptide nucleic acids: a new strategy to interfere in the miRNA maturation.
PNAs conjugated to carrier peptides have been employed for the targeting of miRNA precursor, with the aim to develop molecules able to interfere in the pre-miRNA processing. The capability of the molecules to bind pre-miRNA has been tested in vitro by fluorescence assayes on Thiazole Orange labeled molecules and in vivo, in K562 cells, evaluating the amount of miRNA produced after treatment of cells with two amounts of PNAs.
Dipartimento delle Scienze Biologiche, Università di Napoli "Federico II", Napoli, Italy.
Concetta
C
Avitabile
Michele
M
Saviano
Luca
L
D'Andrea
Nicoletta
N
Bianchi
Enrica
E
Fabbri
Eleonora
E
Brognara
Roberto
R
Gambari
Alessandra
A
Romanelli
D001483:Base Sequence; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D008926:Plicamycin / Q000494:pharmacology
D035683:MicroRNAs; D020135:Peptide Nucleic Acids; D008926:Plicamycin