Tunable biomolecular interaction and fluorescence quenching ability of graphene oxide: application to "turn-on" DNA sensing in biological media.
As a platform for "turn-on" DNA sensing, the level of oxidation of graphene oxide strongly affects its fluorescence quenching ability and binding interactions to single-stranded oligodeoxyribonucleotides (ssODNs), leading to a broad range of sensitivity. Fine-tuning the level of oxidation of graphene oxide yields a DNA-detection platform that is highly sensitive in serum and biological media.
Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.
Bong Jin
BJ
Hong
Zhi
Z
An
Owen C
OC
Compton
SonBinh T
ST
Nguyen
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D005453:Fluorescence; D006108:Graphite / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011806:Quinolinium Compounds / Q000737:chemistry
C481052:Alexa fluor 546; D009838:Oligodeoxyribonucleotides; D011806:Quinolinium Compounds; D006108:Graphite; D004247:DNA