Comparison of mother and child antibodies that target high-molecular-mass Toxoplasma gondii antigens by immunoblotting improves neonatal diagnosis of congenital toxoplasmosis.
This retrospective study proposes a new reading of immunoblotting (IB) in the diagnosis of congenital toxoplasmosis. Our findings demonstrate that a three-IgM-band association at 75, 90, and 100 kDa called the IgM triplet increases the sensitivity to 95.8% when combined with prenatal and serological neonatal tests.
Laboratoire de Parasitologie et Mycologie, Centre Hospitalo-Universitaire de la Timone, Assistance Publique-Hôpitaux de Marseille (AP-HM), Marseille, France. coralie.l'ollivier@gmail.com
Coralie
C
L'Ollivier
Martine
M
Wallon
Benoit
B
Faucher
Renaud
R
Piarroux
François
F
Peyron
Jacqueline
J
Franck
D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000737:chemistry; D005260:Female; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007075:Immunoglobulin M / Q000097:blood; D007231:Infant, Newborn; D008970:Molecular Weight; D011247:Pregnancy; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014122:Toxoplasma / Q000737:chemistry / Q000276:immunology*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007075:Immunoglobulin M