Geraniol synthase whose mRNA is induced by host-selective ACT-toxin in the ACT-toxin-insensitive rough lemon (Citrus jambhiri).
Host-selective toxins (HSTs) produced by some strains of Alternaria alternata are selectively toxic to certain cultivars of plants. However, the role of HSTs in toxin-insensitive plants is currently unknown. Here, we studied the role of ACT-toxin using an ACT-toxin producing A. alternata strain SH20 and the ACT-toxin-insensitive plant rough lemon. Induction of some defense related genes in response to SH20 were faster or stronger than in response to the ACT-toxin deficient SH20 mutant. By sequencing subtractive PCR clones obtained from mRNA of rough lemon leaves inoculated with SH20 after subtraction with that of the ACT-toxin deficient SH20 mutant, we isolated the SH20-responsive genes in rough lemon. Among the SH20-responsive genes analyzed in this study, we isolated a terpene synthase (TPS) gene, RlemTPS3. We also determined that RlemTPS3 localizes to the chloroplast and produces the monoterpene geraniol.
Kagawa University, Miki, Kagawa 761-0795, Japan.
Hodaka
H
Shishido
Yoko
Y
Miyamoto
Rika
R
Ozawa
Shiduku
S
Taniguchi
Junji
J
Takabayashi
Kazuya
K
Akimitsu
Kenji
K
Gomi
D000528:Alternaria / Q000737:chemistry*; D000595:Amino Acid Sequence; D002957:Citrus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006790:Host-Parasite Interactions / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000633:toxicity*; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
D018076:DNA, Complementary; D009183:Mycotoxins; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D010744:Phosphoric Monoester Hydrolases