Nanohybrids of silver particles immobilized on silicate platelet for infected wound healing.
Silver nanoparticles supported on nanoscale silicate platelets (AgNP/NSP) possess interesting properties, including a large surface area and high biocide effectiveness. The nanohybrid of AgNP/NSP at a weight ratio 7/93 contains 5-nm Ag particles supported on the surface of platelets with dimensions of approximately 80×80×1 nm(3). The nanohybrid expresses a trend of lower cytotoxicity at the concentration of 8.75 ppm Ag and low genotoxicity. Compared with conventional silver ions and the organically dispersed AgNPs, the nanohybrid promotes wound healing. We investigated overall wound healing by using acute burn and excision wound healing models. Tests on both infected wound models of mice were compared among the AgNP/NSP, polymer-dispersed AgNPs, the commercially available Aquacel, and silver sulfadiazine. The AgNP/NSP nanohybrid was superior for wound appearance, but had similar wound healing rates, vascular endothelial growth factor (VEGF)-A levels and transforming growth factor (TGF)-β1 expressions to Aquacel and silver sulfadiazine.
Department of Dermatology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Chia-Yu
CY
Chu
Fu-Chuo
FC
Peng
Ying-Fang
YF
Chiu
Hsing-Chuan
HC
Lee
Chien-Wen
CW
Chen
Jiun-Chiou
JC
Wei
Jiang-Jen
JJ
Lin
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D020552:Comet Assay; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D053768:Metal Nanoparticles / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017640:Silicates / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D012837:Silver Sulfadiazine / Q000737:chemistry; D013211:Staphylococcus aureus; D053773:Transforming Growth Factor beta1 / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000097:blood; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D015850:Interleukin-6; D017640:Silicates; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D012834:Silver; D012837:Silver Sulfadiazine