Antimicrobial substantivity over time of chlorhexidine and cetrimide.
BACKGROUND
To reach an adequate control of dentin infection and to promote success in endodontic therapy, antimicrobial irrigating solutions with confirmed substantivity are recommended. The aim of the present study was to evaluate the antimicrobial substantivity against Enterococcus faecalis of a dentin-volumetric unit exposed for 1 minute to chlorhexidine (CHX) and cetrimide (CTR).


METHODS
Standardized coronal dentin blocks of human molars, with and without collagen, were treated for 1 minute with 0.2% and 2% CHX and 0.2% CTR. Afterwards, they were exposed to E. faecalis suspension to determine the antimicrobial substantivity over a period of 60 days. Results were analyzed by means of Kaplan-Meier survival analysis (P < .05).


RESULTS
A direct relationship was seen between CHX concentration and survival time, and the most statistically significant results were obtained in specimens with collagen. CTR showed intermediate survival values close to those of 2% CHX.


CONCLUSIONS
The present study shows that 2% CHX used for 1 minute provides the longest substantivity followed by 0.2% CTR when applied to a dentin-volumetric model.
Department of Preventive Dentistry, School of Dentistry, University of Granada, Granada, Spain. pbaca@ugr.es
Pilar
P
Baca
Pilar
P
Junco
María Teresa
MT
Arias-Moliz
Francisca
F
Castillo
Alberto
A
Rodríguez-Archilla
Carmen María
CM
Ferrer-Luque
D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology*; D002710:Chlorhexidine / Q000494:pharmacology*; D003094:Collagen; D003804:Dentin / Q000737:chemistry / Q000382:microbiology*; D004305:Dose-Response Relationship, Drug; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D053208:Kaplan-Meier Estimate; D012388:Root Canal Irrigants / Q000494:pharmacology*; D013997:Time Factors
D000891:Anti-Infective Agents, Local; D002593:Cetrimonium Compounds; D012388:Root Canal Irrigants; D003094:Collagen; D002710:Chlorhexidine; D000077286:Cetrimonium