Long term estradiol-17β administration changes population of the dorsal root ganglia neurons innervating the ovary in the sexually mature gilts.
The influence of estradiol-17β (E₂) overdose on the number and distribution of neurons in the dorsal root ganglia (DRGs) supplying the ovary of adult pigs was investigated. The numbers of ovarian substance P (SP)-, calcitonin gene-related peptide (CGRP)-, galanin (GAL)-, pituitary adenylate cyclase-activating polypeptide (PACAP)-, neuronal isoform of nitric oxide synthase (nNOS)- and estrogen receptors (ERs)-immunoreactive perikarya were also determined. On day 3 of the estrous cycle, the ovaries of both the control and experimental gilts were injected with retrograde tracer Fast Blue. From day 4 of the estrous cycle to the expected day 20 of the second studied cycle, the experimental gilts were injected with E₂, while the control gilts received oil. The DRGs Th16-L5 were then collected and processed for double-labelling immunofluorescence. Injections of E₂ increased the E₂ level in the peripheral blood ∼4-5-fold and reduced the following in the DRGs: the total number of Fast Blue-positive perikarya, the number of large perikarya, the population of perikarya in the L2 and L3 ganglia, the numbers of SP- and/or CGRP-, PACAP-, nNOS-immunoreactive perikarya and the number of large perikarya expressing ERs subtype α and β. These results show that long-term E₂ treatment of adult gilts affects both the spatial and neurochemical organization pattern of ovary sensory innervation. Our findings suggest that elevated E₂ levels occurring during pathological states may regulate the transmission of sensory modalities from the ovary to the spinal cord.
Division of Reproductive Biology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, 10-747 Olsztyn, Tuwima 10, Poland. baja@pan.olsztyn.pl
Barbara
B
Jana
Michalina
M
Lata
Michał
M
Bulc
Jarosław
J
Całka
D000375:Aging; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D030762:Estrous Cycle / Q000502:physiology; D005260:Female; D019004:Galanin / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D010053:Ovary / Q000187:drug effects* / Q000294:innervation / Q000473:pathology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000378:metabolism; D013552:Swine
D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D004958:Estradiol; D019004:Galanin; D019001:Nitric Oxide Synthase; D015740:Calcitonin Gene-Related Peptide