Draft genome sequence of Streptomyces coelicoflavus ZG0656 reveals the putative biosynthetic gene cluster of acarviostatin family α-amylase inhibitors.
OBJECTIVE
The aims of this study are to obtain the draft genome sequence of Streptomyces coelicoflavus ZG0656, which produces novel acarviostatin family α-amylase inhibitors, and then to reveal the putative acarviostatin-related gene cluster and the biosynthetic pathway.


RESULTS
The draft genome sequence of S. coelicoflavus ZG0656 was generated using a shotgun approach employing a combination of 454 and Solexa sequencing technologies. Genome analysis revealed a putative gene cluster for acarviostatin biosynthesis, termed sct-cluster. The cluster contains 13 acarviostatin synthetic genes, six transporter genes, four starch degrading or transglycosylation enzyme genes and two regulator genes. On the basis of bioinformatic analysis, we proposed a putative biosynthetic pathway of acarviostatins. The intracellular steps produce a structural core, acarviostatin I00-7-P, and the extracellular assemblies lead to diverse acarviostatin end products.


CONCLUSIONS
The draft genome sequence of S. coelicoflavus ZG0656 revealed the putative biosynthetic gene cluster of acarviostatins and a putative pathway of acarviostatin production.


CONCLUSIONS
To our knowledge, S. coelicoflavus ZG0656 is the first strain in this species for which a genome sequence has been reported. The analysis of sct-cluster provided important insights into the biosynthesis of acarviostatins. This work will be a platform for producing novel variants and yield improvement.
TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin, China.
X
X
Guo
P
P
Geng
F
F
Bai
G
G
Bai
T
T
Sun
X
X
Li
L
L
Shi
Q
Q
Zhong
D053898:Biosynthetic Pathways; D016680:Genome, Bacterial; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D000516:alpha-Amylases