Wolbachia infection and dramatic intraspecific mitochondrial DNA divergence in a fig wasp.
Mitochondria and Wolbachia are maternally inherited genomes that exhibit strong linkage disequilibrium in many organisms. We surveyed Wolbachia infections in 187 specimens of the fig wasp species, Ceratosolen solmsi, and found an infection prevalence of 89.3%. DNA sequencing of 20 individuals each from Wolbachia-infected and -uninfected subpopulations revealed extreme mtDNA divergence (up to 9.2% and 15.3% in CO1 and cytochrome b, respectively) between infected and uninfected wasps. Further, mtDNA diversity was significantly reduced within the infected group. Our sequencing of a large part of the mitochondrial genome from both Wolbachia-infected and -uninfected individuals revealed that high sequence divergence is common throughout the mitochondrial genome. These patterns suggest a partial selective sweep of mitochondria subsequent to the introduction of Wolbachia into C. solsmi, by hybrid introgression from a related species.
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Jin-Hua
JH
Xiao
Ning-Xin
NX
Wang
Robert W
RW
Murphy
James
J
Cook
Ling-Yi
LY
Jia
Da-Wei
DW
Huang
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics; D022761:Gene Transfer, Horizontal; D016905:Gram-Negative Bacterial Infections / Q000235:genetics* / Q000382:microbiology; D006239:Haplotypes; D016133:Polymerase Chain Reaction; D014863:Wasps / Q000235:genetics*; D020577:Wolbachia / Q000302:isolation & purification*
D017931:DNA Primers; D004272:DNA, Mitochondrial