Overcharging in biological systems: reversal of electrophoretic mobility of aqueous polyaspartate by multivalent cations.
Charge reversal as an extreme case of charge compensation is directly observed by capillary electrophoresis for a negatively charged peptide in aqueous solutions of trivalent cations. Atomistic and coarse-grained simulations provide molecular interpretation of this effect showing that it is largely of electrostatic origin with a minor contribution of chemical specificity of the salt ions.
Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, Albertov 2030, 12840 Prague 2, Czech Republic.
Anna
A
Kubíčková
Tomáš
T
Křížek
Pavel
P
Coufal
Mario
M
Vazdar
Erik
E
Wernersson
Jan
J
Heyda
Pavel
P
Jungwirth
D002412:Cations / Q000737:chemistry; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010455:Peptides / Q000737:chemistry*; D012996:Solutions; D055672:Static Electricity; D014867:Water / Q000737:chemistry
D002412:Cations; D004573:Electrolytes; D010455:Peptides; D012996:Solutions; D014867:Water; C017645:polyaspartate