Incorporation of a fluorous diazirine group into phosphatidylinositol 4,5-bisphosphate to illustrate its interaction with ADP-ribosylation factor 1.
Phosphatidylinositides are one family of the most versatile signaling molecules in cells, yet how they interact with different proteins to regulate biological processes is not well understood. Towards a general strategy to identify phosphatidylinositide-protein interactions, a fluorous diazirine group has been incorporated into phosphatidylinositol 4,5-bisphosphate (PIP(2)). The modified PIP(2) was effectively cleaved by phospholipase C, one signaling protein that utilizes PIP(2) as its endogenous substrate. Upon light illumination, the PIP(2) probe effectively crosslinks with small GTPase ADP-ribosylation 1 to form a complex, suggesting that the probe might be suitable to identify PIP(2)-interacting proteins on the proteome level.
Division of Chemical Biology and Medicinal Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Weigang
W
Huang
Wei
W
Sun
Zhiquan
Z
Song
Yanbao
Y
Yu
Xian
X
Chen
Qisheng
Q
Zhang
D020823:ADP-Ribosylation Factor 1 / Q000032:analysis / Q000737:chemistry*; D003978:Diazomethane; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000737:chemistry*; D010716:Phosphatidylinositols / Q000737:chemistry*
D005456:Fluorescent Dyes; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D003978:Diazomethane; D020823:ADP-Ribosylation Factor 1