[Molecular epidemiology of genital papillomavirus infection in the Republic of Belarus].
OBJECTIVE
Determination of epidemiologic features of genital HPV infection in women living in the Republic of Belarus, and detection of phylogenetic features of HPV-16.


METHODS
1035 samples of epithelial cervix smears from patients with benign pathology, pre-cancer and cervix cancer were included into the study. 35 samples containing HPV-16 type DNAwere selected for sequencing. HPV L1 gene sequencing (300 n.p.) was performed by using 3100-Avant (Applied Biosystems). Sequencing results analysis was performed by using ClustalX (1.8) and BioEdit. MEGA 4.1 was used for phylogenetic analysis of the fragments obtained and tree construction.


RESULTS
HPV-16 type is a dominant genotype that is responsible for the development of cervix cancer, its frequency of occurrence is 56.7% among patients with the specified pathology living in the Republic of Belarus. Predominance of East-Asian and European HPV-16 subtypes that can be attributed to a single evolutionary branch are the territorial features. At the same time detection of HPV-16 subtypes that have 0.003 - 0.007 p-distance between probes may indicate the presence of the several progenitors circulating in the country. A unique HPV-16 subtype was sequenced in the Gomel Region that could be one of the progenitors of HPV-16 circulating on the European continent.


CONCLUSIONS
Further studies performed in the field of molecular epidemiology will allow to give a qualitative characteristic of the epidemic process, determine the direction and time of introduction of the virus into the country, control the circulation of the virus in the population.
G I
GI
Vergeĭchik
V F
VF
Eremin
D036022:Capsid Proteins / Q000235:genetics*; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics*; D006801:Humans; D017720:Molecular Epidemiology; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000235:genetics*; D002094:Republic of Belarus / Q000453:epidemiology; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000235:genetics*
D036022:Capsid Proteins; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral