Early cartilage degeneration in a rat experimental model of developmental dysplasia of the hip.
Osteoarthritis (OA) is a common long-term complication of developmental dysplasia of the hip (DDH) that is associated with a higher incidence of OA. In addition, the age of onset of OA in DDH patients is significantly younger than in the general population. In order to investigate the early degeneration in DDH cartilage, we used a rat DDH model that was established by the straight-leg swaddling position. The hips were isolated from the DDH model rats and an untreated control group at postnatal weeks 2, 4, 6, and 8. Histology and proteoglycan levels were observed in articular cartilage using Safranin O staining. Biomarkers of cartilage degeneration, including type X collagen and matrix metalloproteinase (MMP)-13, were assessed using immunohistochemistry and quantitative real-time polymerase chain reaction. In addition, expressions of ADAMTS-4 and ADAMTS-5 were studied using quantitative real-time polymerase chain reaction at different ages. DDH rats showed decreased proteoglycans and derangement of chondrocytes when compared with the control group. Collagen X and MMP-13 expressions were higher in the superficial zone of DDH rats than in that of controls (p < 0.05), and the increase was age-dependent. mRNA expression of Collagen X and MMP-13 showed similar results (p < 0.05). A significant increase in mRNA expression of ADAMTS-5 was found in the DDH model cartilage at 8 weeks (p < 0.05). However, no change was observed in ADAMTS-4 expression. This study shows that degenerative cartilage changes occur at an early stage in the rat DDH model and become aggravated with age.
Department of Pediatric Orthopaedic, Children's Hospital of Fudan University, Shanghai, China.
Ning
N
Bo
Wang
W
Peng
Pei
P
Xinghong
Ruixue
R
Ma
D051722:ADAM Proteins / Q000378:metabolism; D000071121:ADAMTS4 Protein; D000071116:ADAMTS5 Protein; D000818:Animals; D001848:Bone Diseases, Developmental / Q000378:metabolism* / Q000473:pathology; D002356:Cartilage / Q000378:metabolism* / Q000473:pathology; D024121:Collagen Type X / Q000096:biosynthesis; D005786:Gene Expression Regulation; D006615:Hip / Q000473:pathology; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis; D015207:Osteoarthritis, Hip / Q000378:metabolism / Q000473:pathology; D011348:Procollagen N-Endopeptidase / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D024121:Collagen Type X; D012333:RNA, Messenger; D051722:ADAM Proteins; D000071116:ADAMTS5 Protein; C518696:Adamts5 protein, rat; D053509:Matrix Metalloproteinase 13; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein