Crossing abilities of ALS target-site resistant black-grass (Alopecurus myosuroides Huds.).
Black-grass crossings were conducted outdoor for two successive years in a way to determine the percentage of resistant black-grass that can be engendered in one generation, after the introduction of one target-site resistant individual. After germination, plants were genotyped and couples of resistant and susceptible individuals were formed, then planted outdoor and enclosed with a pollen-proof cloth to avoid any external pollen contamination. Seeds were collected and tested in glasshouses with herbicide spraying. DNA analyses were also performed on surviving plants to distinguish hetero- from homozygotes. The resistance level of the progeny seems to be fairly high and Mendelean distribution is generally confirmed. The offspring of self-crossings was also observed to assess the facultative autogamy ability, according to the genotype and the presence of mutant ALS alleles (position W-574).
Université de Liège - Gembloux Agro-Bio Tech, Unité de Phytotechnie des Régions Tempérées, Passage des Déportés, 2 B-5030 Gembloux, Belgique. pymarechal@doct.ulg.ac.be
P Y
PY
Maréchal
F
F
Henriet
F
F
Vancutsem
B
B
Bodson
D000095:Acetolactate Synthase / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001947:Breeding; D003433:Crosses, Genetic; D053209:Herbicide Resistance; D006540:Herbicides / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D006109:Poaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*
D006540:Herbicides; D010940:Plant Proteins; D000095:Acetolactate Synthase