[Effect of Euphorbia kansui on urination and kidney AQP2, IL-1beta and TNF-alpha mRNA expression of mice injected with normal saline].
OBJECTIVE
To observe the effect of Euphorbia kansui (E. KS) alcohol extracts on urination and kidney-related expressions of mice injected with normal saline and to discuss its impact on kidney.


METHODS
Mice intraperitoneally injected with normal saline were observed for urination and changes in kidney-related histiocytic factors of after intragastrical administration of E. KS and compared with normal mice.


RESULTS
E. KS alcohol extracts can promote urination of mice injected with normal saline and enhance peripheral serum creatinine, with no obvious pathological change showed in tissue sections. It had a certain effect on reducing AQP2 expression and enhancing TNF-alpha expression.


CONCLUSIONS
Euphorbia kansui in large dose has a remarkable effect on kidney but may be accompanied with pathological reactions to some extent, especially the dose of 1.2 g x kg(-1). The pathological reactions may be related with increased serum creatinine and TNF-alpha expression.
Protein Science Laboratory of Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Huiyu
H
Li
Fan
F
Lei
Yugang
Y
Wang
Xinyue
X
Xiao
Jun
J
Hu
Xianlong
X
Cheng
Dongming
D
Xing
Lei
L
Hua
Ruichao
R
Lin
Lijun
L
Du
D000818:Animals; D051399:Aquaporin 2 / Q000235:genetics*; D028481:Euphorbia; D053583:Interleukin-1beta / Q000235:genetics*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014554:Urination / Q000187:drug effects*
C496024:Aqp2 protein, mouse; D051399:Aquaporin 2; D053583:Interleukin-1beta; D010936:Plant Extracts; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha