Genetic studies of the role of fatty acid and coenzyme A in photocatalytic inactivation of Escherichia coli.
The roles of bacterial cellular components, namely, fatty acid profile and coenzyme A, in photocatalytic inactivation of bacteria were investigated. Escherichia coli BW25113, as a "parental strain", and its isogenic single-gene deletion mutants E. coli JW1081 (fabF(-) mutant) and E. coli JW3942 (coaA(-) mutant) showed different susceptibilities towards photocatalytic inactivation by titanium dioxide (TiO(2), irradiated by UVA lamps (λ = 365 nm)). Regulating the fatty acid composition through pre-incubation temperature adjustment demonstrated the crucial role of cell membrane fatty acid profile in bacterial susceptibility towards photocatalytic inactivation, while the lower coenzyme A level in coaA(-) mutant correlated well with its lower susceptibility towards photocatalytic inactivation. Furthermore, transmission electron microscopic study demonstrated the photocatalytic destruction process of bacterial cells. This is the first study using single-gene deletion mutants to explore better understanding of the photocatalytic inactivation mechanism of E. coli.
School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China.
Minghui
M
Gao
Taicheng
T
An
Guiying
G
Li
Xin
X
Nie
Ho-Yin
HY
Yip
Huijun
H
Zhao
Po-Keung
PK
Wong
D000123:Acetyltransferases / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism; D003065:Coenzyme A / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D029968:Escherichia coli Proteins / Q000235:genetics; D054889:Fatty Acid Synthase, Type II / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism*; D050296:Microbial Viability / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D055668:Photochemical Processes; D013696:Temperature; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology; D014466:Ultraviolet Rays
D029968:Escherichia coli Proteins; D005227:Fatty Acids; C009495:titanium dioxide; D014025:Titanium; D000123:Acetyltransferases; C557137:fabF protein, E coli; D017853:Phosphotransferases (Alcohol Group Acceptor); C023088:pantothenate kinase; D054889:Fatty Acid Synthase, Type II; D003065:Coenzyme A