Apolipoprotein E genotypes, lipid peroxidation, and antioxidant status among mild and severe preeclamptic women from western Iran: protective role of apolipoprotein ε2 allele in severe preeclampsia.
OBJECTIVE
The aim of this study was to examine the association of apolipoprotein E (APOE) genotypes and oxidative stress with the risk of mild and severe preeclampsia.


METHODS
In a case-control study, 198 women with preeclampsia including 128 women with mild and 70 women with severe preeclampsia and 101 control pregnant women from Western Iran were studied. The APOE genotypes were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The serum level of malondialdehyde (MDA) and total antioxidant capacity (TAC) were determined using high-performance liquid chromatography and commercial kits, respectively.


RESULTS
The frequency of APOE ε2 allele in severe preeclamptic women (2.1%) was significantly (p = 0.008) lower than that in controls (9.4%). The presence of APOE ε2 allele was associated with around five times decreased risk of severe preeclampsia [OR = 0.21 (95% CI = 0.6-0.73, p = 0.014)]. A significantly higher serum level of MDA was observed in women with severe (10.87 ± 4.61 μM) and mild (9.81 ± 3.67 μM) preeclampsia compared with that in controls. A trend toward decrease serum level of MDA was observed according to the APOE alleles as ε2 < ε3 < ε4 (9.23, 10.22, and 10.43 μM, respectively). In preeclamptic women, an inverse correlation was detected between serum levels of MDA and HDL-C (r = -0.16, p = 0.029). However, there was a direct correlation between serum level of MDA with diastolic blood pressure (r = 0.15, p = 0.037).


CONCLUSIONS
Our study in a population with Kurdish ethnic background indicates a protective role for APOE ε2 allele against severe preeclampsia that might be through high antioxidant capacity of this allele.
Department of Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Reza
R
Ahmadi
Zohreh
Z
Rahimi
Asad
A
Vaisi-Raygani
Amir
A
Kiani
Nasrin
N
Jalilian
Ziba
Z
Rahimi
D000328:Adult; D001057:Apolipoproteins E / Q000235:genetics*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007492:Iran / Q000453:epidemiology; D015227:Lipid Peroxidation; D008074:Lipoproteins / Q000097:blood; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress; D011225:Pre-Eclampsia / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D012307:Risk Factors; D055815:Young Adult
D001057:Apolipoproteins E; D015415:Biomarkers; D008074:Lipoproteins; D008315:Malondialdehyde