Phosphoproteomics and molecular cardiology: techniques, applications and challenges.
Protein phosphorylation has been widely documented as a key regulatory and signaling mechanism associated with many cardiac diseases. Recent advances in phosphoproteomic technologies such as phosphopeptide enrichment, novel mass spectrometry applications, and bioinformatic tools have resulted in high-throughput identification and quantitation of protein phosphorylation in a global manner. This review summarizes mainstream phosphoproteomic workflows and highlights the most recent applications of phosphoproteomics used in a range of molecular cardiology research.
Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO 80523, USA.
Zeyu
Z
Sun
Karyn L
KL
Hamilton
Kenneth F
KF
Reardon
D000465:Algorithms; D000818:Animals; D006801:Humans; D009206:Myocardium / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000502:physiology
D010750:Phosphoproteins; D020543:Proteome