Coagulation aggravates blood-induced joint damage in dogs.
OBJECTIVE
Joint bleeding due to trauma, major joint surgery, or hemophilia leads to joint damage. It is unclear if there are differences between coagulating blood and anticoagulated blood with respect to joint degeneration, especially in vivo. Therefore, we undertook this study to evaluate in a canine in vivo model whether intraarticular exposure to coagulating blood is more destructive than exposure to anticoagulated blood, and whether inflammation plays a role in the cartilage- damaging process.


METHODS
In 7 dogs the left knees were injected with coagulating blood 4 times a week during weeks 1 and 4, and the right knees were injected with saline. In 7 other dogs, anticoagulated heparinized blood was injected, and heparinized saline was used as control. Ten weeks after the last injection, cartilage matrix turnover and synovial inflammation were analyzed. To study inflammation-independent cartilage damage, explants of cartilage from at least 6 human donors per group were exposed in vitro to coagulating and anticoagulated blood, plasma, and serum for 4 days. Cartilage matrix turnover was determined after a recovery period of 12 days.


RESULTS
Canine knees injected with coagulating blood showed more disturbed proteoglycan turnover than knees injected with anticoagulated blood. Synovial inflammation was present only after intraarticular injections with coagulating blood. In in vitro experiments, exposure of human cartilage explants to coagulating blood resulted in more damage than did exposure to anticoagulated blood, while exposure to plasma and serum did not alter cartilage matrix turnover.


CONCLUSIONS
This study shows that coagulating blood causes more long-lasting in vivo joint damage than anticoagulated blood, thereby suggesting that along with joint bleeding in hemophilia, exposure to intraarticular blood should also be avoided during surgery and trauma to prevent joint damage.
Van Creveld Clinic and University Medical Center Utrecht, Utrecht, The Netherlands. M.E.R.vanMeegeren@umcutrecht.nl
Monique E R
ME
van Meegeren
Goris
G
Roosendaal
Angelique D
AD
Barten-van Rijbroek
Roger E G
RE
Schutgens
Floris P J G
FP
Lafeber
Simon C
SC
Mastbergen
D000368:Aged; D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D002358:Cartilage, Articular / Q000378:metabolism / Q000503:physiopathology*; D004285:Dogs; D005260:Female; D006395:Hemarthrosis / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000503:physiopathology; D007270:Injections, Intra-Articular; D007719:Knee Joint / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D011509:Proteoglycans / Q000378:metabolism*
D011509:Proteoglycans