Effects on groundwater microbial communities of an engineered 30-day in situ exposure to the antibiotic sulfamethoxazole.
Effects upon microbial communities from environmental exposure to concentrations of antibiotics in the μg L(-1) range remain poorly understood. Microbial communities from an oligotrophic aquifer (estimated doubling rates of only once per week) that were previously acclimated (AC) or unacclimated (UAC) to historical sulfamethoxazole (SMX) contamination, and a laboratory-grown Pseudomonas stutzeri strain, were exposed to 240-520 μg L(-1) SMX for 30 days in situ using filter chambers allowing exposure to ambient groundwater, but not to ambient microorganisms. SMX-exposed UAC bacterial communities displayed the greatest mortality and impairment (viable stain assays), the greatest change in sensitivity to SMX (dose-response assays), and the greatest change in community composition (Terminal Restriction Fragment Length Polymorphism; T-RFLP). The sul1 gene, encoding resistance to SMX at clinically relevant levels, and an element of Class I integrons, was not detected in any community. Changes in microbial community structure and SMX resistance over a short experimental period in previously nonexposed, slow-growing aquifer communities suggest concentrations of antibiotics 2-3 orders of magnitude less than those used in clinical applications may influence ecological function through changes in community composition, and could promote antibiotic resistance through selection of naturally resistant bacteria.
Michigan Water Science Center, U.S. Geological Survey, Lansing, Michigan, USA. skhaack@usgs.gov
Sheridan K
SK
Haack
David W
DW
Metge
Lisa R
LR
Fogarty
Michael T
MT
Meyer
Larry B
LB
Barber
Ronald W
RW
Harvey
Denis R
DR
Leblanc
Dana W
DW
Kolpin
D000064:Acclimatization / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D001965:Bromides / Q000032:analysis; D015169:Colony Count, Microbial; D004352:Drug Resistance, Microbial / Q000187:drug effects; D004781:Environmental Exposure / Q000032:analysis*; D060587:Groundwater / Q000382:microbiology*; D059013:Microbial Consortia / Q000187:drug effects*; D050296:Microbial Viability / Q000187:drug effects; D009513:New Hampshire; D025341:Principal Component Analysis; D013420:Sulfamethoxazole / Q000494:pharmacology*; D014871:Water Microbiology
D000900:Anti-Bacterial Agents; D001965:Bromides; D013420:Sulfamethoxazole