Oxidized dextran as crosslinker for chitosan cryogel scaffolds and formation of polyelectrolyte complexes between chitosan and gelatin.
Macroporous scaffolds composed of chitosan and using oxidized dextran as a crosslinker are produced through cryogelation. Introducing gelatin as a third component into the structure results in the formation of mesopores in the pore walls, which are not seen if gelatin is excluded. The mesoporous structure is explained by the formation of polyelectrolyte complexes between chitosan and gelatin before crosslinking takes place. The scaffolds exhibit highly elastic properties withstanding compressions up to 60%. The in vitro biocompatibility of the cryogels is evaluated using fibroblasts from a mouse cell line (L929) and it is seen that the cells adhere and proliferate on the scaffolds. The mesoporous structure seems to have a positive effect on proliferation.
Department of Biotechnology, Lund University, P.O. Box 124, 22100 Lund, Sweden. Dmitry.Berillo@biotek.lu.se
Dmitriy
D
Berillo
Linda
L
Elowsson
Harald
H
Kirsebom
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents; D059985:Cryogels / Q000737:chemistry* / Q000494:pharmacology; D003911:Dextrans / Q000737:chemistry*; D055119:Elastic Modulus; D004573:Electrolytes; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D059985:Cryogels; D003911:Dextrans; D004573:Electrolytes; D010504:Periodic Acid; D005780:Gelatin; C009288:metaperiodate