Virological analysis in patients with human herpes virus 6-associated ocular inflammatory disorders.
OBJECTIVE
To determine whether human herpes virus 6 (HHV-6) genomic DNA and mRNA can be detected in ocular samples from patients with inflammatory disorders, and whether viral replication is involved in the development of inflammation in the eye.


METHODS
After informed consent was obtained, ocular fluid samples (aqueous humor and vitreous fluids) were collected from 350 patients with uveitis or endophthalmitis. Corneal samples were also collected from 65 patients with corneal infections. Multiplex PCR was performed to screen ocular samples from the patients for HHV-1 to HHV-8. Samples were also assayed for HHV-6 DNA using quantitative real-time PCR. Primers for nested RT-PCR were designed to detect amplification of mRNA (HHV-6 A IE1 U90).


RESULTS
PCR results indicated a total of seven patients with uveitis or endophthalmitis (7/350, 2% +) and a single patient with corneal inflammatory disease were positive for HHV-6 DNA (1/65, 1.5% +). These eight patients had high copy numbers of HHV-6 DNA, with values ranging from 4.0 × 10(3) to 5.1 × 10(6) copies/mL. Real-time PCR analysis indicated that two of these cases were HHV-6 variant A and six cases were variant B. In addition, HHV-6 mRNA was clearly detected in vitreous cells collected from one of the patients, suggesting that viral replication may occur in the eye.


CONCLUSIONS
Our results indicate that HHV-6 infection/reactivation is implicated in ocular inflammatory diseases.
Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Chuo-ku, Kobe, Japan. sunaoph@cdb.riken.jp
Sunao
S
Sugita
Norio
N
Shimizu
Ken
K
Watanabe
Manabu
M
Ogawa
Kazuichi
K
Maruyama
Norio
N
Usui
Manabu
M
Mochizuki
D000368:Aged; D001082:Aqueous Humor / Q000737:chemistry*; D003316:Corneal Diseases / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D009877:Endophthalmitis / Q000821:virology; D015817:Eye Infections / Q000821:virology*; D005260:Female; D006566:Herpesviridae Infections / Q000821:virology*; D015654:Herpesvirus 6, Human / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D014605:Uveitis / Q000821:virology; D014822:Vitreous Body / Q000737:chemistry*; D055815:Young Adult
D004279:DNA, Viral; D012333:RNA, Messenger