Psoriasin (S100A7) is a positive regulator of survival and invasion of prostate cancer cells.
OBJECTIVE
Psoriasin, also known as S100A7 and first identified as a protein highly expressed in psoriatic lesions, is a calcium binding protein that has been indicated in various malignancies. The current study aimed to examine the implication of psoriasin in prostate cancer (CaP), particularly its impact on functions of CaP cells.


METHODS
Expression of psoriasin was examined in a variety of prostatic cell lines and human CaP tissues using reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively. Knockdown and overexpression of psoriasin in CaP cells was performed using specifically constructed plasmids, which either had an anti-psoriasin ribozyme transgene or the full-length human S100A7 coding sequence. The effects of manipulating psoriasin expression on cellular functions of CaP cells were assessed using in vitro assays.


RESULTS
Psoriasin was expressed in prostate epithelia and cancer cells. Elevated expression of psoriasin was evident in CaP from its IHC staining in CaP frozen specimens. Psoriasin promoted cell survival under serum starvation. Its expression was inversely correlated with cell-matrix adhesion. Psoriasin increased invasiveness of PC-3 cells via a regulation of matrix metalproteinases (MMPs).


CONCLUSIONS
Aberrant expression of psoriasin is implicated in CaP. Its expression in CaP cells is associated with cell survival, adhesion, and in vitro invasion, which is via the regulation of MMPs.
Metastasis and Angiogenesis Research Group, Institute of Cancer and Genetics, Cardiff University School of Medicine, Heath Park, Cardiff, UK. Electronic address: yel@cf.ac.uk.
Lin
L
Ye
Ping-Hui
PH
Sun
Tracey A
TA
Martin
Andrew J
AJ
Sanders
Malcolm D
MD
Mason
Wen G
WG
Jiang
D017209:Apoptosis / Q000235:genetics; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins / Q000235:genetics* / Q000378:metabolism
D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; C089242:S100A7 protein, human; D019278:Matrix Metalloproteinase 3; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1