Indirect electrochemiluminescence detection of lysine and histidine separated by capillary electrophoresis based on charge displacement.
Indirect electrochemiluminescence (ECL) detection was applied for the analysis of lysine (Lys) and histidine (His) separated by capillary electrophoresis (CE). With the most effective electrophoretic buffer system, which contained 15 mM phosphate buffer (pH = 5.8) and 0.5 mM Tripropylamine (TPA), fast separation of the two basic amino acids could be performed within 7 min. The linear ranges were 10-35 μM, 35-150 μM for Lys; and 5-35 μM, 35-150 μM for His. The detection limits (S/N = 3) were 0.3 μM for Lys and 1.0 μM for His, respectively. The proposed method was also successfully used for the determination of Lys in the oral pharmaceutical formulations.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, China.
Huan
H
Yu
Lei
L
Xu
Tianyan
T
You
D019075:Electrophoresis, Capillary; D006639:Histidine / Q000737:chemistry* / Q000302:isolation & purification; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D008239:Lysine / Q000737:chemistry* / Q000302:isolation & purification
D006639:Histidine; D008239:Lysine