Detection of Brucella spp. DNA in the semen of seronegative bulls by polymerase chain reaction.
Semen samples from 88 reproductively mature bulls were screened to detect the presence of Brucella spp. by polymerase chain reaction. Twenty-seven samples were found to be positive, underscoring the importance of researching brucellosis in males and the need for greater care in the selection of sperm-donating bulls for semen centres.
Faculty of Veterinary Medicine, Collaborating Centre of Agricultural Protection in Central Brazil, Federal University of Uberlândia, Uberlândia, MG, Brazil. dan_hp2002@yahoo.com.br
D G
DG
Junqueira Junior
G M S
GM
Rosinha
C E G
CE
Carvalho
C E
CE
Oliveira
C C
CC
Sanches
A M C
AM
Lima-Ribeiro
D000818:Animals; D002002:Brucella / Q000235:genetics / Q000302:isolation & purification*; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology; D004269:DNA, Bacterial / Q000302:isolation & purification*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012661:Semen / Q000382:microbiology*; D012698:Serologic Tests
D004269:DNA, Bacterial