Microarray-based gene profiling analysis of Müller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish.
Microarray-based gene profiling has become an important technique to measure changes in gene expression on a genome-wide scale. Recently, cell-specific microarrays have been reported to study changes in gene expression of a particular cell type in several model organisms. Here, we describe a protocol to prepare RNA samples for microarray analysis of isolated Müller glia-derived retinal stem cells from light-damaged adult zebrafish expressing a fluorescent marker in Müller cells using enzymatic retinal dissociation followed by fluorescence-activated cell sorting (FACS).
Department of Molecular, Cellular, and Developmental Biology, University of Michigan College of Literature, Science, and the Arts, Ann Arbor, MI, USA.
Zhao
Z
Qin
Pamela A
PA
Raymond
D000818:Animals; D005434:Flow Cytometry; D020869:Gene Expression Profiling / Q000379:methods*; D008027:Light / Q000009:adverse effects*; D009457:Neuroglia / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012160:Retina / Q000166:cytology / Q000293:injuries / Q000378:metabolism* / Q000528:radiation effects; D013234:Stem Cells / Q000378:metabolism*; D015027:Zebrafish