Stable expression of Y-box protein 1 gene in early development of the abalone Haliotis diversicolor.
Abalone animals are import models for the study of the early development of marine invertebrates. However, systematical evaluations of internal control genes (ICG) have seldomly been performed. In this study, ten candidate genes were cloned and surveyed for their stability throughout the early developmental period of H. diversicolor using qPCR. In a period from fertilized egg to postlarva, three genes, Y-box protein 1 (YB1), ornithine decarboxylase antizyme 1 (OAZ1) and eukaryotic translation initiation factor 5A (EIF5A), were found to be the most stable and could be used as ICGs. It is suggested that using two genes jointly, such as YB1 and OAZ1, could be sufficiently reliable to normalize the temporal dynamics of other genes. Normalized by YB1/OAZ1, some rough features of early development of a small abalone were characterized. This is the first report of the temporal dynamics of metabolic activities and overall mRNA abundance of abalone animals in early stages. It is also the first time the multi-functional gene YB1 has been described as an internal control for early developmental biology studies. Phylogeny and function of YB1 are further discussed.
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China. chenjun@xmu.edu.cn
Jun
J
Chen
Zhi-Sen
ZS
Chen
Zi-Xia
ZX
Huang
Cai-Huan
CH
Ke
Jie
J
Zhang
Yi-Xiang
YX
Zhong
Wei-Wei
WW
You
Jing
J
Zhao
D000818:Animals; D003001:Cloning, Molecular; D049851:Gastropoda / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000166:cytology / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051840:Y-Box-Binding Protein 1 / Q000235:genetics* / Q000378:metabolism; D000097574:Eukaryotic Translation Initiation Factor 5A
D010448:Peptide Initiation Factors; D011506:Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D051840:Y-Box-Binding Protein 1; D000097574:Eukaryotic Translation Initiation Factor 5A; C031017:ornithine decarboxylase antizyme