Effect of glyphosate-based herbicide on acetylcholinesterase activity in tadpoles, Hoplobatrachus rugulosus.
This study focused on the effects of a glyphosate-based herbicide on activity of the neuron enzyme, acetylcholinesterase (AchE), in the tadpole stage (stage 35-39) of the East Asian Bullfrog, Hoplobatrachus rugulosus. There were 4 herbicide concentration treatments consisting of glyphosate-based herbicide added at 21, 24, 27 and 30 microl to 1L de-chlorinated water in glass containers (10x15x20 cm). There were 4 replicates per treatment, each replicate using 20 tadpoles. The toxicity results were compared with tadpoles in distilled water as a control treatment. After 24, 48, 72 and 96 hours exposure to glyphosate-based herbicide concentrations, LC50 values of 25.21, 24.66, 24.16 and 23.63 microl/L, respectively, were recorded. AChE activities decreased significantly and markedly with herbicide concentration. Such inhibition of AChE activity by this glyphosate-based herbicide indicates the potential of such herbicides to disrupt ecological communities in water near where the herbicides are applied.
Department of Zoology, Faculty of Science, Kasetsart University, 50 Phahon Yothin Road, Chatuchak, Bangkok 10900, Thailand. rwatchar@su.ac.th
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Ruamthum
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Visetson
J R
JR
Milne
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Bullangpoti
D000818:Animals; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D004334:Drug Administration Schedule; D005998:Glycine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000008:administration & dosage / Q000633:toxicity*; D007814:Larva / Q000201:enzymology / Q000378:metabolism; D011892:Rana catesbeiana / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*; D000097797:Glyphosate
D002800:Cholinesterase Inhibitors; D005998:Glycine; D006540:Herbicides; D014874:Water Pollutants, Chemical; D000097797:Glyphosate