Electron tomographic analysis of synaptic ultrastructure.
Synaptic function depends on interactions among sets of proteins that assemble into complex supramolecular machines. Molecular biology, electrophysiology, and live-cell imaging studies have provided tantalizing glimpses into the inner workings of the synapse, but fundamental questions remain regarding the functional organization of these "nano-machines." Electron tomography reveals the internal structure of synapses in three dimensions with exceptional spatial resolution. Here we report results from an electron tomographic study of axospinous synapses in neocortex and hippocampus of the adult rat, based on aldehyde-fixed material stabilized with tannic acid in lieu of postfixation with osmium tetroxide. Our results provide a new window into the structural basis of excitatory synaptic processing in the mammalian brain.
Department of Cell & Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA. alain.burette@gmail.com
Alain C
AC
Burette
Thomas
T
Lesperance
John
J
Crum
Maryann
M
Martone
Niels
N
Volkmann
Mark H
MH
Ellisman
Richard J
RJ
Weinberg
D000818:Animals; D016477:Artifacts; D049229:Dendritic Spines / Q000502:physiology / Q000648:ultrastructure; D055032:Electron Microscope Tomography / Q000379:methods*; D008297:Male; D009993:Osmium Tetroxide; D057907:Post-Synaptic Density / Q000502:physiology / Q000648:ultrastructure; D017729:Presynaptic Terminals / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure*
D009993:Osmium Tetroxide