Clinical and histologic evaluation of effects of hyaluronic acid in rat tongue.
OBJECTIVE
This study aimed to investigate local and systemic effects of 2 concentrations of hyaluronic acid (HA) in rats.


METHODS
Fifty-four female Wistar rats were allocated to 3 treatment groups (25 mg/mL HA, 5.5 mg/mL HA, and 0.9% NaCl) and 3 subgroups of experimental time (7, 60, and 90 days). The rats were evaluated clinically and then killed. The tongue and right kidney were removed and stained by hematoxylin and eosin and picrosirius (tongue only).


RESULTS
The clinical changes did not differ significantly between groups (Kruskal-Wallis: P > .05). Histologically there was significantly greater inflammatory response in the groups with HA compared with control (Kruskal-Wallis: P < .001). All kidney samples were normal. The 25 mg/mL HA group had higher collagen formation (analysis of variance mixed models: P = .016).


CONCLUSIONS
HA causes moderate inflammatory reaction to 7 days and mild to 60 and 90 days, which is compatible with tissue repair, and a slightly increased collagen formation around the material was detected.
Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil.
Ruchielli
R
Loureiro Borghetti
Karlon Fróes
KF
de Vargas
Sabrina
S
Pozatti Moure
Fernanda
F
Gonçalves Salum
Maria Antonia Zancanaro
MA
de Figueiredo
D000038:Abscess / Q000139:chemically induced; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis; D003238:Connective Tissue / Q000187:drug effects; D003357:Cosmetic Techniques; D004305:Dose-Response Relationship, Drug; D005260:Female; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014059:Tongue / Q000187:drug effects* / Q000378:metabolism; D055682:Viscoelastic Substances / Q000008:administration & dosage / Q000494:pharmacology*
D001672:Biocompatible Materials; D055682:Viscoelastic Substances; D006820:Hyaluronic Acid; D003094:Collagen