Nanoparticle based galectin-1 gene silencing, implications in methamphetamine regulation of HIV-1 infection in monocyte derived macrophages.
Galectin-1, an adhesion molecule, is expressed in macrophages and implicated in human immunodeficiency virus (HIV-1) viral adsorption. In this study, we investigated the effects of methamphetamine on galectin-1 production in human monocyte derived macrophages (MDM) and the role of galectin-1 in methamphetamine potentiation of HIV-1 infection. Herein we show that levels of galectin-1 gene and protein expression are significantly increased by methamphetamine. Furthermore, concomitant incubation of MDM with galectin-1 and methamphetamine facilitates HIV-1 infection compared to galectin-1 alone or methamphetamine alone. We utilized a nanotechnology approach that uses gold nanorod (GNR)-galectin-1 siRNA complexes (nanoplexes) to inhibit gene expression for galectin-1. Nanoplexes significantly silenced gene expression for galectin-1 and reversed the effects of methamphetamine on galectin-1 gene expression. Moreover, the effects of methamphetamine on HIV-1 infection were attenuated in the presence of the nanoplex in MDM.
Department of Medicine, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Innovation Center, 640 Ellicott Street, Buffalo, NY 14203, USA. jlr8@buffalo.edu
Jessica L
JL
Reynolds
Wing Cheung
WC
Law
Supriya D
SD
Mahajan
Ravikumar
R
Aalinkeel
Bindukumar
B
Nair
Donald E
DE
Sykes
Ken-Tye
KT
Yong
Rui
R
Hui
Paras N
PN
Prasad
Stanley A
SA
Schwartz
D037483:Galectin 1 / Q000096:biosynthesis* / Q000235:genetics; D020868:Gene Silencing / Q000187:drug effects / Q000502:physiology*; D015658:HIV Infections / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D008694:Methamphetamine / Q000494:pharmacology / Q000627:therapeutic use*; D053758:Nanoparticles / Q000627:therapeutic use*; D034741:RNA, Small Interfering / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use
D037483:Galectin 1; D034741:RNA, Small Interfering; D008694:Methamphetamine