[Analysis of DNA damage/repair level in Rutilus rutilus L. from reservoirs of the Techa River cascade with different levels of radiactive pollution].
The Comet Assay and micronucleus assays have been used to evaluate the condition of the nuclear DNA in erythrocytes of peripheral blood of roach (Rutilus rutilus L.) from water-storage of low-level radioactive waste. The Rutilus rutilus L. from the Shershny reservoir, Chelyabinsk, was used as a control population. Radionuclide maintenance in water, sediments and roach in those reservoirs and Shershny reservoir was defined. The dose rate for Rutilus rutilus L. was calculated using program complex ERICA Assessment Tool 1.0 May 2009. Our investigation has shown that a chronic radiation of population (dose rate - 5.2 mGy/day and 19.5 mGy/day) leads to a significantly higher level of the DNA damage in erythrocytes of peripheral blood and increases the speed of nuclear DNA reparation after irradiation of erythrocytes in vitro. We suppose that it may be a result of the increased quantity of active form of oxygen in cells of the fish in water-storage of low-level radioactive waste.
E V
EV
Stiazhkina
N A
NA
Obvintseva
I A
IA
Shaposhnikova
G A
GA
Triapitsyna
P M
PM
Stukalov
E A
EA
Priakhin
D000818:Animals; D020552:Comet Assay / Q000379:methods; D003530:Cyprinidae / Q000235:genetics*; D004247:DNA / Q000097:blood* / Q000235:genetics; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D015162:Micronucleus Tests; D011868:Radioisotopes / Q000032:analysis; D045483:Rivers; D012426:Russia; D014875:Water Pollutants, Radioactive / Q000009:adverse effects*
D011868:Radioisotopes; D014875:Water Pollutants, Radioactive; D004247:DNA