DNA polyfluorophores for real-time multicolor tracking of dynamic biological systems.
Dye-ing to live: Spectral limitations of common organic dyes make it difficult or impossible to visualize and follow multiple biological components in rapidly moving systems. The development of a multispectral set of improved DNA-scaffolded fluorophores is described. Their use in multicolor cellular imaging (see scheme) and in tracking of biological motions on the subsecond timescale is demonstrated.
Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
Shenliang
S
Wang
Jia
J
Guo
Toshikazu
T
Ono
Eric T
ET
Kool
D000818:Animals; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry*; D048488:Paramecium caudatum / Q000166:cytology; D015027:Zebrafish / Q000196:embryology
D005456:Fluorescent Dyes; D009841:Oligonucleotides; D004247:DNA