In vitro anti-Candida activity of lidocaine and nitroglycerin: alone and combined.
The aim of this work was to study the anti-Candida activity of lidocaine and nitroglycerin alone and in combination. Ten Candida strains were included, corresponding to 1 collection type strain (ATCC 10231) and 9 clinical isolates: 4 C. albicans, 2 C. glabrata, 1 C. tropicalis, 1 C. krusei, and 1 C. parapsilosis. The CLSI reference M27-A3 micromethod was used to determine the anti-Candida activity of the drugs alone; minimal inhibitory and lethal concentrations were determined. The classic checkboard technique was used to determine the activity of combined drugs. Lidocaine fungicidal effect was dosedependent. Nitroglycerin exhibited a higher effect. The drugs combination resulted in a reduction of the inhibitory concentration, corresponding to an additive effect. In conclusion, both drugs exhibited an interesting anti-Candida activity. The combination of lidocaine with nitroglycerin was shown to have an additive effect against Candida spp., predicting the interest to include, in the future, these drugs in a new delivery system for the treatment of mucocutaneous candidosis.
Health Sciences Research Center, Faculty of Health Sciences, University of Beira Interior, Covilhã, Portugal. apo@fcsaude.ubi.pt
Ana
A
Palmeira-de-Oliveira
Ana Rita
AR
Ramos
Carlos
C
Gaspar
Rita
R
Palmeira-de-Oliveira
Paula
P
Gouveia
José
J
Martinez-de-Oliveira
D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D004359:Drug Therapy, Combination; D008012:Lidocaine / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D005996:Nitroglycerin / Q000494:pharmacology*
D000935:Antifungal Agents; D008012:Lidocaine; D005996:Nitroglycerin