Postoperative adhesion prevention with a new barrier: an experimental study.
BACKGROUND
Postoperative adhesion formation remains a major clinical problem. The aim of this study was to test the effect of a new hydrogel on adhesion formation in a rat model.


METHODS
A reproducible rat model was used to induce standardized adhesion formation in three experiments. In experiment 1, a cross-linked polyvinyl alcohol (PVA) and carboxymethylcellulose (CMC) hydrogel (PVA/CMC, A-Part®; B. Braun Aesculap, Germany) was tested in different dosages. In experiment 2, PVA/CMC gel was compared to icodextrin 4% (Adept®; Baxter USA). In both groups, animals were sacrificed after 2 weeks. In experiment 3, histological examination after 4 and 6 weeks was performed. The percentage of adhesions to the defect was measured and the density was determined according to the Zühlke scale. During histological examination of the abdominal wall, the formation of neoperitoneum and potential residues of the agents were assessed.


RESULTS
In experiment 1, a significant reduction in amount as well as density of the adhesions was visible with all dosages of PVA/CMC gel. In experiment 2, again quantity and density of the adhesions were diminished by PVA/CMC hydrogel compared to the control group. Icodextrin 4% showed no significant reduction in adhesion formation. In experiment 3, no residues of PVA/CMC gel or icodextrin 4% were found during histological examination after 4 and 6 weeks and neoperitoneum was present in all cases.


CONCLUSIONS
PVA/CMC hydrogel appears to be a novel effective adhesion prevention agent. Together with an upcoming safety study, these data encourage to start clinical efficacy studies.
Department of Surgery, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands. m.ditzel@erasmusmc.nl
M
M
Ditzel
E B
EB
Deerenberg
N
N
Komen
I M
IM
Mulder
H
H
Jeekel
J F
JF
Lange
D000818:Animals; D002266:Carboxymethylcellulose Sodium; D005260:Female; D020100:Hydrogels / Q000627:therapeutic use*; D023421:Models, Animal; D011142:Polyvinyl Alcohol; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D000267:Tissue Adhesions / Q000517:prevention & control*
D020100:Hydrogels; C062364:polyvinyl alcohol hydrogel; D011142:Polyvinyl Alcohol; D002266:Carboxymethylcellulose Sodium