Alteration of carbohydrates metabolism and midgut glucose absorption in Gromphadorhina portentosa after subchronic exposure to imidacloprid and fenitrothion.
This study was undertaken to test the hypothesis that following exposure to insecticides, changes take place in the metabolism of carbohydrates and absorption in the midgut of insects. The Madagascar hissing cockroach (Gromphadorhina portentosa) was chosen for the experiment as a model organism, due to it being easy to breed and its relatively large alimentary tract, which was important when preparing the microperfusion midgut bioassay. In each group of cockroaches treated with imidacloprid and fenitrothion, absorption of glucose, expressed as the area under the curve (AUC), was elevated compared to the control group. Glucose in the hemolymph of the examined insects was present in a vestigial amount, often below the threshold of determination, so the determinable carbohydrate indices were: hemolymph trehalose concentration and fat body glycogen content. The level of trehalose found in the hemolymph of insects when exposed to fenitrothion, and irrespective of the level of concentration mixed into food, were significantly lower when comparing to the control samples. Imidacloprid acted analogically with one exception at the concentration of 10 mg·kg(-1) dry food where trehalose concentration did not differ from the control values. Coupling with fat body glycogen concentration was less visible and appeared only at the concentrations of 5 and 10 mg imidacloprid·kg(-1) dry food. As described in this study changes in the sugar distribution and midgut glucose absorption indicate that insects cover the increased energy needs induced by insecticides; also at the gastrointestinal tract level. The result indicates that the midgut glucose absorption parameters could be considered as a non-specific biomarker of insecticide toxicity.
Department of Physiology in Zabrze, Medical University of Silesia, Jordana, Zabrze, Poland. sawczyn.t@gmail.com
Tomasz
T
Sawczyn
Bogdan
B
Dolezych
Marcin
M
Klosok
Maria
M
Augustyniak
Dominika
D
Stygar
Rafal J
RJ
Buldak
Michal
M
Kukla
Katarzyna
K
Michalczyk
Iwona
I
Karcz-Socha
Krystyna
K
Zwirska-Korczala
D000042:Absorption; D000818:Animals; D002855:Chromatography, Thin Layer; D003058:Cockroaches / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005216:Fat Body / Q000187:drug effects / Q000378:metabolism; D005278:Fenitrothion / Q000008:administration & dosage / Q000633:toxicity*; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D007093:Imidazoles / Q000008:administration & dosage / Q000633:toxicity*; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000008:administration & dosage / Q000633:toxicity*; D014199:Trehalose / Q000378:metabolism
D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; C082359:imidacloprid; D006003:Glycogen; D014199:Trehalose; D005947:Glucose; D005278:Fenitrothion