[Analysis of a 452C/T single nucleotide polymorphism in γ-glutamyl hydrolase gene in children with acute leukemia].
OBJECTIVE
To investigate the distribution of γ-glutamyl hydrolase gene (GGH) 452C/T genotype and allele frequency in children with acute leukemia (AL) and healthy children.


METHODS
Bone marrow samples from 92 children with AL and peripheral blood samples from 124 healthy children were obtained to prepare complementary DNAs (cDNAs). The cDNAs were analyzed for a GGH 452C/T polymorphism by reverse transcriptase-polymerase chain reaction-denaturing gradient gel electrophoresis (RT-PCR-DGGE) and direct sequencing.


RESULTS
The frequencies of the AL patients with TT, CT and CC genotypes were 2.2%, 13.0% and 84.8%, and the frequencies of the control children were 1.6%, 16.9% and 81.5%, respectively. There was no significant difference in GGH genotype or T allele frequency between the two groups (P> 0.05). However, the T allele frequency in Han Chinese children was significantly different from those reported in Japanese, Mexican and African-American populations.


CONCLUSIONS
The frequency of 452C/T polymorphism of GGH gene in Han Chinese children has been determined. The results suggested that an ethnic difference may exist.
Shenzhen Children's Hospital of Zunyi Medical College, Shenzhen, Guangdong 518026, P. R. China.
Hong-hong
HH
Zhang
Li-jie
LJ
Yue
Xiao-wen
XW
Chen
Wei
W
Zhao
Chun-yan
CY
Hu
Miao-miao
MM
Zheng
Chun-lan
CL
Yang
Cai
C
Xie
D000208:Acute Disease; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007938:Leukemia / Q000201:enzymology / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011623:gamma-Glutamyl Hydrolase / Q000235:genetics*
D011623:gamma-Glutamyl Hydrolase