Methylglyoxal inhibition of cytosolic ascorbate peroxidase from Nicotiana tabacum.
Methylglyoxal (MG) is one of the aldehydes accumulated in plants under environmental stress. Cytosolic ascorbate peroxidase (cAPX) plays a key role in the protection of cells from oxidative damage by scavenging reactive oxygen species in higher plants. A cDNA encoding cAPX, named NtcAPX, was isolated from Nicotiana tabacum. We characterized recombinant NtcAPX (rNtcAPX) as a fusion protein with glutathione S-transferase to investigate the effects of MG on APX. NtcAPX consists of 250 amino acids and has a deduced molecular mass of 27.5 kDa. The rNtcAPX showed a higher APX activity. MG treatments resulted in a reduction of APX activity and modifications of amino groups in rNtcAPX with increasing K(m) for ascorbate. On the contrary, neither NaCl nor cadmium reduced the activity of APX. The present study suggests that inhibition of APX is in part due to the modification of amino acids by MG.
Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
Md Anamul
MA
Hoque
Misugi
M
Uraji
Akiko
A
Torii
Mst Nasrin Akhter
MN
Banu
Izumi C
IC
Mori
Yoshimasa
Y
Nakamura
Yoshiyuki
Y
Murata
D060387:Ascorbate Peroxidases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000737:chemistry; D002104:Cadmium / Q000737:chemistry; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004795:Enzyme Stability; D004926:Escherichia coli; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000737:chemistry; D010940:Plant Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000737:chemistry; D011765:Pyruvaldehyde / Q000494:pharmacology*; D012965:Sodium Chloride / Q000737:chemistry; D014026:Nicotiana / Q000166:cytology / Q000201:enzymology*
D004791:Enzyme Inhibitors; D010940:Plant Proteins; D002104:Cadmium; D012965:Sodium Chloride; D011765:Pyruvaldehyde; D060387:Ascorbate Peroxidases; D008274:Magnesium