Comparison of the Application of Allogeneic Fibroblast and Autologous Mesh Grafting With the Conventional Method in the Treatment of Third-Degree Burns.
Wound healing is a multipart process involving different cell types and growth factors. Third-degree burns are usually treated by early excision and skin grafting. Tissue engineering has been developed in this field in response to limitations associated with autografts. Allogeneic fibroblasts on meshed split thickness skin grafts (STSGs) are known to have useful properties in wound healing and can be used to construct a new model of living skin substitute. Fourteen patients were chosen from June 2009 until December 2010 as the sample for this study. After debridement and wound excision, meshed STSG was used to cover the entire wound. Alloskin (allofibroblasts cultured on a combination of silicone and glycosaminoglycan) was applied on one side and petroleum jelly-impregnated gauze (Iran Polymer and Petrochemical Institute) was applied on the other. The healing time, scar formation, and pigmentation score were assessed for the patients. All analyses were undertaken with SPSS 17 software. Alloskin demonstrated good properties compared to petroleum jelly-impregnated gauze. The average healing time and hypertrophic scar formation were significantly different between the two groups. In addition, the skin pigmentation score in the alloskin group was closer to normal. Alloskin grafting, including fibroblasts on meshed STSG, may be a useful method to reduce healing time and scar size and may require less autologous STSG in extensive burns where a high percentage of skin is burned and there is a lack of available donor sites.
From the *Skin Research Center, Department of Dermatology, and †Plastic and Reconstructive Surgery Department, 15th Khordad Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran; ‡Surgery Department, Ayatollah Mousavi Hospital, §Microbiology Department, and ‖Surgery Department, Ayatollah Mousavi Hospital, Zanjan University of Medical Sciences, Zanjan, Iran; and ¶Polymeric Biomaterials Department, Iran Polymer and Petrochemical Institute (IPPI), Tehran, Iran.
Hamideh
H
Moravvej
Abdoljalil Kalantar
AK
Hormozi
Seyed Nejat
SN
Hosseini
Rahim
R
Sorouri
Naser
N
Mozafari
Mohammad Reza
MR
Ghazisaidi
Mahnaz Mahmoudi
MM
Rad
Mohammad Hossein
MH
Moghimi
Shahin Mohammad
SM
Sadeghi
Hamid
H
Mirzadeh
D000328:Adult; D001684:Biological Dressings; D002056:Burns / Q000209:etiology / Q000473:pathology* / Q000601:surgery*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008297:Male; D016038:Skin Transplantation; D017768:Skin, Artificial; D016896:Treatment Outcome; D014945:Wound Healing