Effect of addition of coconut water (Cocos nucifera) to the freezing media on post-thaw viability of boar sperm.
The aims of this experiment were to evaluate the addition of coconut water in natura to the freezing media, compare the effect of deionized water vs filtered water of coconut over the post-thaw seminal characteristics, and evaluate the effect of the deionized water and in natura coconut water on the seminal characteristics of boar sperm at different post-thaw times. Thirty-four ejaculates were used divided in three aliquots which received one of the following treatments (T): T1, LEY (bidistilled water, lactose, and egg yolk) and LEYGO (LEY + glycerol and Orvus ET paste); T2, LEY(A) (coconut deionized water, lactose, and egg yolk)-LEYGO(A); and T3, LEY(B) (in natura coconut water, lactose, and egg yolk)-LEYGO(B). Samples of boar semen were frozen according to the Westendorf method, thawed at 38°C, and evaluated at three incubation times (0, 30, and 60 min). Seminal characteristics assessed were motility (Mot), acrosomal integrity (AInt), membrane integrity (MInt), and mitochondrial activity (MAct). T1 showed a higher percentage of viable sperm than T3 (Mot 36.5 vs 5.4 %, AInt 61.8 vs 41.2 %, MInt 50.4 vs 41.3 %, and MAct 56.9 vs 50.5 %). T2 kept a higher percentage of viable sperm at all incubation times. In natura coconut water showed a detrimental effect over the viability of the frozen-thawed boar semen. Deionized coconut water improved the boar semen viability post-thaw, outperforming results of in natura coconut water.
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, km 15.5 Carretera Mérida-Xmatkuil, CP 97100, Mérida, Yucatán, México.
María
M
Bottini-Luzardo
Fernando
F
Centurión-Castro
Militza
M
Alfaro-Gamboa
Ricardo
R
Aké-López
José
J
Herrera-Camacho
D000177:Acrosome / Q000187:drug effects; D000704:Analysis of Variance; D000818:Animals; D003059:Cocos / Q000737:chemistry*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000032:analysis / Q000494:pharmacology*; D003470:Culture Media / Q000032:analysis / Q000494:pharmacology*; D004530:Egg Yolk; D007785:Lactose; D008297:Male; D008928:Mitochondria / Q000187:drug effects; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects*; D034421:Sus scrofa
D003451:Cryoprotective Agents; D003470:Culture Media; D007785:Lactose