Comparison of separation performances of novel β-cyclodextrin-based chiral stationary phases in high-performance liquid chromatographic enantioseparation.
Three β-cyclodextrin-based chiral stationary phases were developed applying novel bonding chemistry. The separation performances of β-cyclodextrin, (R,S)-2-hydroxypropyl-β-cyclodextrin, and permethyl-β-cyclodextrin-based CSPs were compared in the resolution of structurally divergent analytes, such as coumarins, dansyl amino acids, and propionic acid derivatives. Separations were carried out in reversed phase mode applying 0.1% triethylammonium phosphate (pH 3.5)/MeOH mobile phase systems in different compositions. Of the three novel CSPs the permethyl-β-cyclodextrin bonded phase proved to be the most effective one for the enantioseparation of investigated analytes.
ChiroQuest Chiral Technologies Development Ltd., Rumbach S. u. 7, H-1075 Budapest, Hungary.
Gábor
G
Varga
Gábor
G
Fodor
István
I
Ilisz
Julianna
J
Szemán
Júlia
J
Visy
Lajos
L
Szente
Antal
A
Péter
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry; D003505:Cyclodextrins / Q000737:chemistry*; D003619:Dansyl Compounds / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D011422:Propionates / Q000032:analysis* / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D002021:Buffers; D003374:Coumarins; D003505:Cyclodextrins; D003619:Dansyl Compounds; D011422:Propionates; D000644:Quaternary Ammonium Compounds; D012997:Solvents; D047392:beta-Cyclodextrins; C487420:heptakis(2,3,6-tri-O-methyl)cyclomaltoheptaose; C066585:triethylammonium cation; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000432:Methanol