Adoptive immunity mediated by HLA-A*0201 restricted Asp f16 peptides-specific CD8+ T cells against Aspergillus fumigatus infection.
Aspergillus fumigatus (A. fumigatus) is the most common pathogen of invasive aspergillosis (IA), a life-threatening infection in immunocompromised patients. Recent findings revealed that CD8+ T cells can mediate cytotoxic activity against A. fumigatus. Here, we bioinformatically identified three HLA-A*0201-restricted peptides from Asp f16, an A. fumigatus antigen which was previously shown to be involved in T cell immunity. Our immunological results demonstrated that these peptides can potently induce cytotoxic T lymphocyte (CTL) response in CD8+ T cells, thus, damaging the conidia and hyphae of A. fumigatus. Moreover, the Asp f16 peptides can also raise Th1 cell-like response, as measured by interferon-γ (IFN-γ) enzyme-linked immunosorbent spot (ELISPOT). Furthermore, we established an invasive pulmonary aspergillosis model in HLA-A*0201 transgenic mice. Adoptive transfer of Asp f16 peptides-specific CTL significantly extended the overall survival time in the A. fumigatus-infected immunocompromised mice. In conclusion, our results demonstrate that the Asp f16 peptides might provide immunity against invasive A. fumigatus infection.
Department of Dermatology and Venereology, Peking University First Hospital, Beijing, 100034, People's Republic of China.
Z
Z
Sun
P
P
Zhu
L
L
Li
Z
Z
Wan
Z
Z
Zhao
R
R
Li
D056704:Adaptive Immunity; D019264:Adoptive Transfer; D000485:Allergens / Q000276:immunology*; D000818:Animals; D001228:Aspergillosis / Q000276:immunology*; D001232:Aspergillus fumigatus / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D019295:Computational Biology; D004195:Disease Models, Animal; D058501:Enzyme-Linked Immunospot Assay; D005656:Fungal Proteins / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000378:metabolism*; D025301:Hyphae / Q000276:immunology; D007116:Immunization, Passive; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013172:Spores, Fungal / Q000276:immunology; D016019:Survival Analysis; D018417:Th1 Cells / Q000276:immunology
D000485:Allergens; D005656:Fungal Proteins; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; C438315:allergen Asp f 16; D007371:Interferon-gamma