Colorimetric detection of urine glucose based ZnFe2O4 magnetic nanoparticles.
In this paper, we discovered that ZnFe(2)O(4) magnetic nanoparticles (MNPs) possess intrinsic peroxidase-like activity. ZnFe(2)O(4) MNPs exhibit several advantages such as high catalytic efficiency, good stability, monodispersion, and rapid separation over other peroxidase nanomimetics and horseradish peroxidase (HRP). ZnFe(2)O(4) MNPs were used as a colorimetric biosensor for the detection of urine glucose. This method is simple, inexpensive, highly sensitive, and selective for glucose detection using glucose oxidase (GOx) and ZnFe(2)O(4) MNPs with a linear range from 1.25 × 10(-6) to 1.875 × 10(-5) mol L(-1) with a detection limit of 3.0 × 10(-7) mol L(-1). The color change observable by the naked eyes based on the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) is the principle for the sensing of urine glucose level.
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, China.
Li
L
Su
Jie
J
Feng
Ximin
X
Zhou
Cuiling
C
Ren
Honghong
H
Li
Xingguo
X
Chen
D001560:Benzidines / Q000032:analysis; D040761:Biomimetic Materials / Q000737:chemistry* / Q000378:metabolism; D002863:Chromogenic Compounds / Q000032:analysis; D003124:Colorimetry / Q000191:economics / Q000379:methods*; D005296:Ferrous Compounds / Q000737:chemistry* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006029:Glycosuria / Q000652:urine*; D006801:Humans; D057230:Limit of Detection; D008280:Magnetics; D008297:Male; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D017967:Zinc Compounds / Q000737:chemistry* / Q000378:metabolism
D001560:Benzidines; D002863:Chromogenic Compounds; D005296:Ferrous Compounds; D017967:Zinc Compounds; C021758:3,3',5,5'-tetramethylbenzidine; D005949:Glucose Oxidase; D009195:Peroxidase; D005947:Glucose