Sensitive detection of whole-genome differentiation among closely-related species of the genus Fusarium using DNA-DNA hybridization and a microplate technique.
We developed a new system for detection of whole-genome differentiation using DNA-DNA hybridization, and tested its sensitivity with three closely-related Fusarium species. We compared DNA-DNA relatedness to nucleotide sequence homologies of five genetic regions between each of five strains of three Fusarium species. DNA-DNA relatedness by our system was 16.2-86.6%. Sequence homologies of 18S rDNA, rDNA cluster region from ITS1 to 28S rDNA, β-tub, EF-1α and lys2 were 100.0, 99.0-100.0, 96.7-100.0, 95.1-99.4, and 94.7-100.0%, respectively. Our system could clearly detect differentiation between closely-related fungal species which have very similar morphological-characteristics, and exhibit little diagnoses in nucleotide sequences. Our results suggest that this system is a good tool for identification and phylogenetic analysis of closely-related fungal species.
Division of Microbiology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, Japan. : mwatanabe@nihs.go.jp
Maiko
M
Watanabe
Keiichi
K
Goto
Yoshiko
Y
Sugita-Konishi
Yoichi
Y
Kamata
Yukiko
Y
Hara-Kudo
D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005670:Fusarium / Q000145:classification / Q000235:genetics*; D016681:Genome, Fungal; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020648:Peptide Elongation Factor 1 / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000737:chemistry / Q000235:genetics
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D020648:Peptide Elongation Factor 1; D012337:RNA, Ribosomal, 18S; D014404:Tubulin; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase