Synthesis, pH-induced "on-off-on" luminescence switching, and partially intercalative DNA-binding and DNA photocleavage properties of an β-D-allopyranoside-grafted ruthenium(II) complex.
A new ruthenium(II) complex [Ru(Happip)(3)](ClO(4))(2) {Happip=2-(4-(β-D-allopyranoside)phenyl)imidazo[4,5-f][1,10]phenanthroline} was synthesized and characterized by elemental analysis, (1)H NMR and matrix-assisted laser desorption ionization mass spectrometry. Calf-thymus DNA-binding properties were studied by DNA viscosity measurements and spectroscopic methods of UV-visible (UV-vis) absorption and luminescence titrations, steady-state emission quenching, DNA competitive binding with ethidium bromide and DNA melting experiments, indicating that the complex partially intercalates into the DNA with a large binding constant greater than 10(6)M(-1). The pH effects on UV-vis absorption and emission spectra of the complex were studied, demonstrating that the complex acted as an excellent pH-induced "on-off-on" luminescence switch with large on-off intensity ratios of 88 and 50 with one of luminescence on/off switching actions occurring in near-physiological pH region (pK(a2)=7.33). The DNA photocleaving properties of [Ru(Happip)(3)](2+) were also studied and compared with those of [Ru(bpy)(2)(Happip)](2+) and [Ru(bpy)(2)(Hpip)](2+){bpy=2,2'-bipyridine, Hpip=2-(4-phenyl)imidazo[4,5-f][1,10]phenanthroline}.
College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
Xiao-Long
XL
Zhao
Yan-Zi
YZ
Ma
Ke-Zhi
KZ
Wang
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives; D001667:Binding, Competitive; D056831:Coordination Complexes / Q000138:chemical synthesis*; D004247:DNA / Q000737:chemistry; D004996:Ethidium / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007364:Intercalating Agents / Q000138:chemical synthesis*; D007700:Kinetics; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D010618:Phenanthrolines / Q000737:chemistry; D010782:Photolysis; D012428:Ruthenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014466:Ultraviolet Rays
D056831:Coordination Complexes; D006027:Glycosides; D007364:Intercalating Agents; D010618:Phenanthrolines; D015082:2,2'-Dipyridyl; D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium; C025205:1,10-phenanthroline