Development of a strategy for biological monitoring in a chemical plant producing 3,3'-dichlorobenzidine dihydrochloride.
In a chemical plant in Germany producing 3,3'-dichlorobenzidine dihydrochloride for the manufacture of colorants, blood and urine samples were taken for biological monitoring. 3,3'-Dichlorobenzidine (DBZ) was analyzed in urine by thin-layer chromatography and subsequently further combined with analysis of adducts of 3,3'-DBZ in hemoglobin. Data highlight current ranges of industrial exposure to 3,3'-DBZ in Germany and demonstrate the applicability of biological monitoring to minimize this exposure. Effective biological monitoring was achieved by a combination of monitoring hemoglobin adducts with spot samplings of urinary 3,3'-DBZ excretion in cases of reported exposure periods. Data presented might help to identify biological guidance values (BGV/BAR) for 3,3'-DBZ-exposed individuals.
Leibniz Research Centre for Working Environment and Human Factors (IfADo), Dortmund, Germany.
Kristian F
KF
Knoell
Norbert
N
Will
Gabriele
G
Leng
Silvia
S
Selinski
Jan G
JG
Hengstler
Klaus
K
Golka
Hermann M
HM
Bolt
D015101:3,3'-Dichlorobenzidine / Q000032:analysis*; D002273:Carcinogens / Q000032:analysis*; D002617:Chemical Industry; D002855:Chromatography, Thin Layer; D004784:Environmental Monitoring / Q000379:methods*; D062665:Epidemiological Monitoring; D005858:Germany / Q000453:epidemiology; D017408:Guidelines as Topic; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D016273:Occupational Exposure / Q000009:adverse effects
D002273:Carcinogens; D006454:Hemoglobins; D015101:3,3'-Dichlorobenzidine