CTAB-coated gold nanorods elicit allergic response through degranulation and cell death in human basophils.
The effect of CTAB (cetyltrimethylammonium bromide)- or PEG (polyethylene glycol)-coated gold-nanorods (Au-NRs) on the non-IgE mediated allergic response was studied. We found that the CTAB-Au-NRs released more allergic mediators such as histamine and β-hexosaminidase from human basophil KU812, a common model for studying allergy, after 20 min incubation. Also, the CTAB-Au-NRs induced more apoptosis than the PEG-Au-NRs in KU812 24 h after treatment. These short- and long-term effects were not solely due to the CTAB residues in the supernatant desorbed from the Au-NRs.
Programme of Biochemistry, School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong (CUHK), Shatin, NT, Hong Kong. kalun2004@cuhk.edu.hk
Ka Lun
KL
Cheung
Huanjun
H
Chen
Qiulan
Q
Chen
Jianfang
J
Wang
Ho Pui
HP
Ho
Chun Kwok
CK
Wong
Siu Kai
SK
Kong
D017209:Apoptosis / Q000187:drug effects*; D001491:Basophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006632:Histamine / Q000378:metabolism; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D043942:Nanotubes / Q000737:chemistry / Q000633:toxicity*; D011092:Polyethylene Glycols / Q000737:chemistry; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D002593:Cetrimonium Compounds; D011092:Polyethylene Glycols; D006046:Gold; D006632:Histamine; D001619:beta-N-Acetylhexosaminidases; D000077286:Cetrimonium