Structural characterisation and identification of phenylethanoid glycosides from Cistanches deserticola Y.C. Ma by UHPLC/ESI-QTOF-MS/MS.
BACKGROUND
Phenylethanoid glycosides (PhGs) are the major active constituents of Cistanches deserticola Y.C. Ma. However, the isolation, purification and identification procedures of PhGs are difficult and time-consuming.


OBJECTIVE
To establish a rapid and sensitive ultrahigh-pressure liquid chromatography (UHPLC)/ESI (electrospray ion source)-quadrupole time of flight (QTOF)-MS/MS method that could be applied to rapidly profile and identify PhGs.


METHODS
Seven standard compounds were used for the investigation of the fragmentation pattern. Based on the UHPLC/ESI-QTOF-MS/MS method, the important structural information on the types of aglycone and saccharide sequences present should be obtained.


RESULTS
According to the HPLC retention behaviour, the proposed fragmentation pathways provided by high-resolution MS and MS/MS spectra and literature sources, a total of 13 PhGs in the crude extract of C. deserticola were identified or tentatively identified.


CONCLUSIONS
A rapid and accurate UHPLC/ESI-QTOF-MS/MS method was established for the identification of PhGs in the crude extract of C. deserticola. This method therefore can be used for rapid prediction of the chemical constituents and qualities of this plant.
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Tianjin, 300193, PR China.
Lifeng
L
Han
Mavis
M
Boakye-Yiadom
Erwei
E
Liu
Yi
Y
Zhang
Wei
W
Li
Xinbo
X
Song
Fenghua
F
Fu
Xiumei
X
Gao
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D031671:Cistanche / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D006027:Glycosides; D010936:Plant Extracts