DNA barcodes for insects.
DNA barcoding refers to the technique of sequencing a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene, the "DNA barcode," from a taxonomically unknown specimen and performing comparisons with a reference library of barcodes of known species origin to establish a species-level identification. The library barcodes gain their value due to an intimate association-through the vouchered specimens from where they came-with other data; particularly Linnaean names, collection localities, and morphology in the form of digital images. Consequently, this chapter details means of efficiently obtaining barcodes along two general streams: rapid barcode assembly to populate the library and retrieval of barcodes from highly prized specimens, but also emphasizes organization and collection of the barcode collaterals.
Biodiversity Institute of Ontario, University of Guelph, Guelph, ON, Canada. jwilso04@uoguelph.ca
John James
JJ
Wilson
D000818:Animals; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D007313:Insecta / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004247:DNA