Cytotoxic effect of MTA and CEM cement in human gingival fibroblast cells. Scanning electronic microscope evaluation.
In this ex-vivo study, we assessed the adhesion of human gingival fibroblast (HGF) to mineral trioxide aggregate (MTA) and calcium-enriched mixture (CEM) cement using a scanning electronic microscope (SEM). Test biomaterials were prepared and inserted in polyethylene wells (n = 18). Glass coverslips were used for control groups. HGFs were seeded in the glass coverslips, MTA and CEM. For the positive-control group, distilled water was added to the medium; the samples were observed with SEM at one, three and seven-day intervals. Normal cell morphology was observed in the negative controls. In the positive controls, round cells with rougher surfaces were seen; these cells showed few attachments to the glass coverslip. HGFs spread and adhered similarly on MTA and CEM by forming a monolayer of flat cells; there were no statistical differences between these two experimental groups. HGF cells displayed a favorable biologic response in contact with MTA and CEM. This demonstrates non-cytotoxicity of CEM as a novel endodontic biomaterial.
Iranian Center for Endodontic Research, Shahid Beheshti University of Medical Sciences, Tehran, Iran. saasgary@yahoo.com
Saeed
S
Asgary
Seyed Hasan
SH
Moosavi
Zahra
Z
Yadegari
Shahriar
S
Shahriari
D017607:Aluminum Compounds / Q000633:toxicity*; D001672:Biocompatible Materials / Q000633:toxicity; D017610:Calcium Compounds / Q000633:toxicity*; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003738:Dental Cements / Q000633:toxicity*; D004338:Drug Combinations; D005347:Fibroblasts / Q000187:drug effects* / Q000648:ultrastructure; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000633:toxicity*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D013997:Time Factors
D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D003738:Dental Cements; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate