Genetic characterization of Mycobacterium tuberculosis in the West Bank, Palestinian Territories.
BACKGROUND
The World Health Organization (WHO) declared human tuberculosis (TB) a global health emergency and launched the "Global Plan to Stop Tuberculosis" which aims to save a million lives by 2015. Global control of TB is increasingly dependent on rapid and accurate genetic typing of species of the Mycobacterium tuberculosis (MTB) complex including M. tuberculosis. The aim of this study was to identify and genetically characterize the MTB isolates circulating in the West Bank, Palestinian Territories. Genotyping of the MTB isolates from patients with pulmonary TB was carried out using two molecular genetic techniques, spoligotyping and mycobacterial interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) supported by analysis of the MTB specific deletion 1 (TbD1).


RESULTS
A total of 17 MTB patterns were obtained from the 31 clinical isolates analyzed by spoligotyping; corresponding to 2 orphans and 15 shared-types (SITs). Fourteen SITs matched a preexisting shared-type in the SITVIT2 database, whereas a single shared-type SIT3348 was newly created. The most common spoligotyping profile was SIT53 (T1 variant), identified in 35.5 % of the TB cases studied. Genetic characterization of 22 clinical isolates via the 15 loci MIRU-VNTR typing distinguished 19 patterns. The 15-loci MIT144 and MIT145 were newly created within this study. Both methods determined the present of M. bovis strains among the isolates.


CONCLUSIONS
Significant diversity among the MTB isolates circulating in the West Bank was identified with SIT53-T1 genotype being the most frequent strain. Our results are used as reference database of the strains circulating in our region and may facilitate the implementation of an efficient TB control program.
Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, Palestine. sereqat@med.alquds.edu
Suheir
S
Ereqat
Abedelmajeed
A
Nasereddin
Kifaya
K
Azmi
Ziad
Z
Abdeen
Charles L
CL
Greenblatt
Mark
M
Spigelman
Nalin
N
Rastogi
Gila Kahila
GK
Bar-Gal
D000995:Antitubercular Agents / Q000627:therapeutic use; D015373:Bacterial Typing Techniques / Q000379:methods; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D030541:Databases, Genetic; D024881:Drug Resistance, Bacterial / Q000235:genetics; D017353:Gene Deletion; D005838:Genotype; D006801:Humans; D020071:Interspersed Repetitive Sequences; D008877:Middle East / Q000453:epidemiology; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology; D009154:Mutation; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*
D000995:Antitubercular Agents; D004269:DNA, Bacterial