Matrix effects in analysis of β-agonists with LC-MS/MS: influence of analyte concentration, sample source, and SPE type.
The synergistic influences of analyte concentration, sample source, and solid-phase extraction (SPE) type on matrix effects in the multiresidue analyses of eight β-agonists with LC-ESI-MS/MS were evaluated. Porcine muscle and liver extracts and urine from diverse sources were purified by strong or mixed-mode cation exchange and molecularly imprinted polymer SPE cartridges, respectively. Three spiked concentrations (2, 10, and 20 ng/mL) of eight β-agonists in the purified matrices and the different sample sources were analyzed. The results show that for most β-agonists there are significant differences in matrix effects between analyte concentrations or sample sources (P < 0.05), whereas there is no significant difference in matrix effects between different SPE cartridges (P > 0.05). Results from main effects testing indicated that analyte concentration was the main effector.
National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine,  South China Agricultural University , Guangzhou 510642, China.
Li-Qi
LQ
Wang
Zhen-Ling
ZL
Zeng
Yi-Juan
YJ
Su
Gao-Kui
GK
Zhang
Xiu-Ling
XL
Zhong
Zheng-Peng
ZP
Liang
Li-Min
LM
He
D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000302:isolation & purification; D000327:Adsorption; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008099:Liver / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods; D014556:Urine / Q000737:chemistry*
D000318:Adrenergic beta-Agonists; D011108:Polymers