Crystallization and preliminary crystallographic analysis of D-aspartate oxidase from porcine kidney.
D-Aspartate oxidase (DDO) from porcine kidney was crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as a precipitant. The crystal belonged to space group P2(1), with unit-cell parameters a = 79.38, b = 144.0, c = 80.46 Å, β = 101.1°, and diffracted to 1.80 Å resolution. Molecular-replacement trials using the structure of human D-amino-acid oxidase, which is 42% identical in sequence to DDO, as a search model provided a satisfactory solution.
Structure Guided Drug Development Project, JBIC Research Institute, Japan Biological Informatics Consortium, 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan. toshiya-senda@aist.go.jp
Miki
M
Senda
Atsushi
A
Yamamoto
Hiroyuki
H
Tanaka
Tetsuo
T
Ishida
Kihachiro
K
Horiike
Toshiya
T
Senda
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D050804:D-Aspartate Oxidase / Q000737:chemistry*; D007668:Kidney / Q000201:enzymology*; D013552:Swine
D050804:D-Aspartate Oxidase