A vesiculovirus showing a steepened transcription gradient and dominant trans-repression of virus transcription.
Vesicular stomatitis virus (VSV) is a prototype nonsegmented, negative-sense virus used to examine viral functions of a broad family of viruses, including human pathogens. Here we demonstrate that S(2) VSV, an isolate with a small plaque phenotype compared to other Indiana strain viruses, has a transcription defect resulting in an altered pattern and rapid decline of transcription. The S(2) VSV transcription gradient is dominant over the wild-type transcription in a coinfection. This is the first characterization of an altered gradient of transcription not dependent on RNA template sequence or host response and may provide insight into new approaches to viral attenuation.
Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts, USA.
Erin N
EN
Hodges
Bianca S
BS
Heinrich
John H
JH
Connor
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D015967:Gene Expression Regulation, Viral; D012367:RNA, Viral / Q000096:biosynthesis; D014158:Transcription, Genetic; D018116:Vesiculovirus / Q000502:physiology*; D014779:Virus Replication
D012367:RNA, Viral