Initial crystallographic studies of a small heat-shock protein from Xylella fastidiosa.
The ORF XF2234 in the Xylella fastidiosa genome was identified as encoding a small heat-shock protein of 17.9 kDa (HSP17.9). HSP17.9 was found as one of the proteins that are induced during X. fastidiosa proliferation and infection in citrus culture. Recombinant HSP17.9 was crystallized and surface atomic force microscopy experiments were conducted with the aim of better characterizing the HSP17.9 crystals. X-ray diffraction data were collected at 2.7 Å resolution. The crystal belonged to space group P4(3)22, with unit-cell parameters a = 68.90, b = 68.90, c = 72.51 Å, and is the first small heat-shock protein to crystallize in this space group.
Institute of Biology, Molecular Biology and Genetic Engineering Centre, University of Campinas, CP 6010, 13083-875 Campinas-SP, Brazil.
Susely F S
SF
Tada
Antonio Marcos
AM
Saraiva
Gabriela S
GS
Lorite
Luciana K
LK
Rosselli-Murai
Alexandre César
AC
Pelloso
Marcelo Leite
ML
dos Santos
Daniela B B
DB
Trivella
Mônica A
MA
Cotta
Anete Pereira
AP
de Souza
Ricardo
R
Aparicio
D018360:Crystallography, X-Ray; D050888:Heat-Shock Proteins, Small / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D044167:Xylella / Q000737:chemistry*
D050888:Heat-Shock Proteins, Small