Hydrogen may inhibit collagen-induced platelet aggregation: an ex vivo and in vivo study.
OBJECTIVE
Hydrogen selectively reduces hydroxyl radicals and peroxynitrite, and numerous experimental and clinical studies suggest that hydrogen can exert potent cellular protective effects against a wide variety of diseases. Furthermore, there is increasing evidence that antioxidants can modulate platelet activation. The aim of the present study was to investigate the relationship between hydrogen and collagen-induced platelet aggregation.


METHODS
For human ex vivo studies, we collected blood samples from six healthy humans and added normal saline or hydrogen-rich saline to blood and platelet-rich plasma. We found that collagen (1 µg/mL)-induced platelet aggregation was significantly inhibited by hydrogen-rich saline compared with a normal saline group (p=0.044). For rat in vivo studies, animals (n=17) were exposed to either nitrogen-based mixed gas with hydrogen (H2 gas group; n=9) or without hydrogen (non-H2 gas group; n=8). Additionally, another animals (n=13) administered either normal (NS group; n=7) or hydrogen-rich saline (HS group; n=6) (5 ml/kg) via intravenous infusion. Blood samples were drawn from the vena cava before treatment and from the right ventricle after treatment. Collagen (12 µg/mL)-induced platelet aggregation was then measured.


RESULTS
Collagen-induced platelet aggregation was significantly decreased in H2 gas and HS group rats (p=0.042, 0.018, respectively), while there was no difference in non-H2 gas and NS group rats before and after treatment.


CONCLUSIONS
In summary, these data suggest that hydrogen may inhibit collagen-induced platelet aggregation.
Department of Neurosurgery, National Defense Medical College, Japan. s.takeuchi@room.ocn.ne.jp
Satoru
S
Takeuchi
Kojiro
K
Wada
Kimihiro
K
Nagatani
Hideo
H
Osada
Naoki
N
Otani
Hiroshi
H
Nawashiro
D000328:Adult; D000818:Animals; D003094:Collagen / Q000494:pharmacology; D005260:Female; D006801:Humans; D006859:Hydrogen / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D066298:In Vitro Techniques; D007262:Infusions, Intravenous; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000097:blood
D013481:Superoxides; D006859:Hydrogen; D003094:Collagen