Structural characterization and immunostimulatory activity of a novel linear α-(1→6)-D-glucan isolated from Panax ginseng C. A. Meyer.
Panax ginseng C. A. Meyer is a well-known plant medicine in the world. Ginseng polysaccharides mainly contain starch-like glucan and pectin. In this paper, a novel glucan WGPA-UH-N1 was purified from ginseng pectin by the treatment of de-esterification and endo-polygalacturonase, followed by the chromatographies on DEAE-Sepharose Fast Flow and Sephadex G-50 column. WGPA-UH-N1 has molecular weight about 17 kDa. WGPA-UH-N1 was determined to be a linear α-(1→6)-D-glucan without side chains by FT-IR, (13)C-NMR, (1)H-NMR, HMQC and HMBC spectra. It is the first time to isolate a linear α-(1→6)-D-glucan from Panax ginseng C. A. Meyer. Immunological activity assays showed that WGPA-UH-N1, although not effective on the phagocytosis of macrophage, could significantly induce lymphocyte proliferation without mitogenic stimuli at 1.0 mg/mL or with LPS at 0.5 mg/mL, also significantly increase NO production at the range of 0.1-1.0 mg/mL in a dose-dependent manner. The immunological activities of WGPA-UH-N1 are different from those of the β-(1→6)-D-glucan (BIWP2) isolated from the fruit bodies of Bulgaria Inquinans (Fries).
School of Life Sciences, Northeast Normal University, Changchun, People's Republic of China.
Lin
L
Sun
Xiaoxia
X
Peng
Pan
P
Sun
Jiahong
J
Shi
Xiaowen
X
Yuan
Jingjing
J
Zhu
Guihua
G
Tai
Yifa
Y
Zhou
D000818:Animals; D001203:Ascomycota / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002845:Chromatography; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D009569:Nitric Oxide; D005894:Panax / Q000737:chemistry*; D010587:Phagocytosis / Q000187:drug effects; D011096:Polygalacturonase / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D005936:Glucans; D007155:Immunologic Factors; D009569:Nitric Oxide; D011096:Polygalacturonase