Thermal inactivation kinetics of recombinant proteins of the lipoxygenase pathway related to the synthesis of virgin olive oil volatile compounds.
The aim of this work was to characterize the thermal inactivation parameters of recombinant proteins related to the biosynthesis of virgin olive oil (VOO) volatile compounds through the lipoxygenase (LOX) pathway. Three purified LOX isoforms (Oep2LOX1, Oep1LOX2, and Oep2LOX2) and a hydroperoxide lyase (HPL) protein (OepHPL) were studied. According to their thermal inactivation parameters, recombinant Oep1LOX2 and Oep2LOX2 could be identified as the two LOX isoforms active in olive fruit crude preparations responsible for the synthesis of 13-hydroperoxides, the main substrates for the synthesis of VOO volatile compounds. Recombinant Oep2LOX1 displayed a low thermal stability, which suggests a weak actuation during the oil extraction process considering the current thermal conditions of this industrial process. In addition, recombinant OepHPL could be identified as the HPL activity in crude preparations. The thermal stability was the highest among the recombinant proteins studied, which suggests that HPL activity is not a limiting factor for the synthesis of VOO volatile compounds.
Department of Physiology and Technology of Plant Products, Instituto de la Grasa, CSIC, Seville, Spain.
María N
MN
Padilla
José M
JM
Martínez-Rivas
Ana G
AG
Pérez
Carlos
C
Sanz
D000446:Aldehyde-Lyases / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004795:Enzyme Stability; D005511:Food Handling / Q000379:methods; D006358:Hot Temperature; D007700:Kinetics; D008084:Lipoxygenase / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D011994:Recombinant Proteins / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000378:metabolism*
D000069463:Olive Oil; D010938:Plant Oils; D011994:Recombinant Proteins; D055549:Volatile Organic Compounds; D003577:Cytochrome P-450 Enzyme System; D008084:Lipoxygenase; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase