A transgenic Bm cell line of piggyBac transposon-derived targeting expression of humanized glycoproteins through N-glycosylation.
Glycoproteins have been implicated in a wide variety of important biochemical and biological functions, including protein stability, immune function, enzymatic function, cellular adhesion and others. Unfortunately, there is no therapeutic protein produced in insect system to date, due to the expressed glycoproteins are paucimannosidic N-glycans, rather than the complex, terminally sialylated N-glycans in mammalian cells. In this paper, we cloned the necessary genes in glycosylation of mammalian cells, such as N-acetylglucosaminyltransferase II (Gn-TII), galactosyltransferases (Gal-Ts), 2,6-Sial-T (ST6 GalII)and 2,3-Sial-T (ST3GalIII), and transformed them to silkworm genome of BmN cell line through transgenesis to establish a transgenic Bm cell line of piggyBac transposon-derived targeting expression of humanized glycoproteins. The study supplied a new insect cell line which is practically to produce "bisected" complex N-glycans like in mammalian cells.
Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
Jia-Biao
JB
Hu
Peng
P
Zhang
Mei-Xian
MX
Wang
Fang
F
Zhou
Yan-Shan
YS
Niu
Yun-Gen
YG
Miao
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D023061:Gene Order; D018390:Gene Targeting; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D010957:Plasmids; D011134:Polysaccharides / Q000378:metabolism; D014170:Transformation, Genetic; D019076:Transgenes
D004251:DNA Transposable Elements; D006023:Glycoproteins; D011134:Polysaccharides