Caffeic acid phenethyl ester protects 661W cells from H2O2-mediated cell death and enhances electroretinography response in dim-reared albino rats.
OBJECTIVE
Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H(2)O(2)-mediated cell death and in albino rats (in vivo) against various light conditions.


METHODS
The 661W cells were pretreated with CAPE and then stressed with H(2)O(2). Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02%) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding.


RESULTS
Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light.


CONCLUSIONS
CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats' retinas.
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Hui
H
Chen
Julie-Thu A
JT
Tran
Robert E
RE
Anderson
Md Nawajes A
MN
Mandal
D000417:Albinism; D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects / Q000235:genetics; D004596:Electroretinography; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000032:analysis; D015870:Gene Expression / Q000187:drug effects*; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D008027:Light; D058945:Lipoxygenases / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D020419:Photoreceptor Cells, Vertebrate / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011429:Propolis / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D000099169:Fos-Related Antigen 1
D002109:Caffeic Acids; D005136:Eye Proteins; D015525:Fatty Acids, Omega-3; D051547:Heme Oxygenase-1; D006861:Hydrogen Peroxide; D018799:Intercellular Adhesion Molecule-1; D007770:L-Lactate Dehydrogenase; D058945:Lipoxygenases; D016328:NF-kappa B; D010626:Phenylethyl Alcohol; D011429:Propolis; D020011:Protective Agents; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D000099169:Fos-Related Antigen 1; C090509:Icam1 protein, mouse; C055494:caffeic acid phenethyl ester