Dopaminergic cell populations of the rat substantia nigra are differentially affected by essential fatty acid dietary restriction over two generations.
Essential fatty acids play a crucial role in the activity of several neurotransmission systems, especially in the monoaminergic systems involved in cognitive and motor aspects of behavior. The present study investigated whether essential fatty acid dietary restriction over two generations could differentially affect dopaminergic cell populations located in the substantia nigra rostro-dorso-medial (SNrm) or caudo-ventro-lateral (SNcv) regions which display distinct neurochemical profile and vulnerability to lesions under selected pathological conditions. Wistar rats were raised from conception on control or experimental diets containing adequate or reduced levels of linoleic and α-linolenic fatty acids, respectively. Stereological methods were used to estimate both the number and soma size of tyrosine hydroxylase (TH)-immunoreactive neurons in the SNrm and SNcv. TH protein levels were assessed with Western blots. Long-term treatment with the experimental diet modified the fatty acid profile of midbrain phospholipids and significantly decreased TH protein levels in the ventral midbrain (3 fold), the number of TH-positive cells in the SNrm (∼20%) and the soma size of these neurons in both SNrm (∼20%) and SNcv (∼10%). The results demonstrate for the first time a differential sensitivity of two substantia nigra dopaminergic cell populations to unbalanced levels of essential fatty acids, indicating a higher vulnerability of SNrm to the harmful effects induced by docosahexaenoic acid brain deficiency.
Departamento de Fisiologia e Farmacologia, Centro de Ciências Biológicas, CCB, Universidade Federal de Pernambuco, Cidade Universitária, Recife, PE, Brazil.
Priscila Pereira
PP
Passos
Juliana Maria Carrazone
JM
Borba
Ana Paula
AP
Rocha-de-Melo
Rubem Carlos Araujo
RC
Guedes
Reginaldo Pereira
RP
da Silva
Waldenício Teixeira Melo
WT
Filho
Kátia Maria Marques
KM
Gouveia
Daniela Maria do Amaral Ferraz
DM
Navarro
Geanne Karla Novais
GK
Santos
Roseane
R
Borner
Cristovam Wanderley
CW
Picanço-Diniz
Antonio
A
Pereira
Miriam Stela Maris
MS
de Oliveira Costa
Marcelo Cairrão Araújo
MC
Rodrigues
Belmira Lara da Silveira
BL
Andrade-da-Costa
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000254:growth & development / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D048429:Cell Size; D018752:Diet, Fat-Restricted; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D059290:Dopaminergic Neurons / Q000276:immunology / Q000378:metabolism*; D005228:Fatty Acids, Essential / Q000008:administration & dosage / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007150:Immunohistochemistry; D019787:Linoleic Acid / Q000008:administration & dosage / Q000378:metabolism; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D051381:Rats; D017208:Rats, Wistar; D013238:Stereotaxic Techniques; D013378:Substantia Nigra / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology / Q000378:metabolism*; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000378:metabolism
D005228:Fatty Acids, Essential; D017962:alpha-Linolenic Acid; D004281:Docosahexaenoic Acids; D019787:Linoleic Acid; D014446:Tyrosine 3-Monooxygenase