Aminotriazole attenuated carbon tetrachloride-induced oxidative liver injury in mice.
Carbon tetrachloride (CCl(4)) has been used extensively to study xenobiotic-induced oxidative liver injury. Catalase (CAT) is a major antioxidant enzyme while aminotriazole (ATZ) is commonly used as a CAT inhibitor. In the present study, the effects of ATZ on CCl(4)-induced liver injury were investigated. Our experimental data showed that pretreatment with ATZ significantly decreased CCl(4)-induced elevation of serum aspartate transaminase (AST) and alanine transaminase (ALT) and improved hepatic histopathological abnormality. ATZ dose-dependently inhibited the activity of CAT, but it reduced the content of H(2)O(2) and the levels of malondialdehyde (MDA) in liver tissues. ATZ decreased plasma level of pro-inflammatory cytokines (TNF-α and IL-6) and reduced hepatic levels of myeloperoxidase (MPO). In addition, posttreatment with ATZ also decreased the level of ALT and AST. These data indicated that ATZ effectively alleviated CCl(4)-induced oxidative liver damage. These findings suggested that ATZ might have potential value in preventing oxidative liver injury.
Department of Pathophysiology, Chongqing Medical University, Chongqing, China.
Xinyu
X
Deng
Kunwei
K
Wu
Jingyuan
J
Wan
Longjiang
L
Li
Rong
R
Jiang
Mengying
M
Jia
Yuping
Y
Jing
Li
L
Zhang
D000410:Alanine Transaminase / Q000097:blood; D000640:Amitrole / Q000494:pharmacology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002374:Catalase / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000201:enzymology / Q000517:prevention & control*; D016207:Cytokines / Q000097:blood; D004305:Dose-Response Relationship, Drug; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress
D016207:Cytokines; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D002374:Catalase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000640:Amitrole