Measurement of intrinsic properties of amyloid fibrils by the peak force QNM method.
We report the investigation of the mechanical properties of different types of amyloid fibrils by the peak force quantitative nanomechanical (PF-QNM) technique. We demonstrate that this technique correctly measures the Young's modulus independent of the polymorphic state and the cross-sectional structural details of the fibrils, and we show that values for amyloid fibrils assembled from heptapeptides, α-synuclein, Aβ(1-42), insulin, β-lactoglobulin, lysozyme, ovalbumin, Tau protein and bovine serum albumin all fall in the range of 2-4 GPa.
ETH Zurich, Food & Soft Materials Science, Institute of Food, Nutrition & Health, Schmelzbergstrasse 9, LFO E23, 8092 Zurich, Switzerland.
Jozef
J
Adamcik
Cecile
C
Lara
Ivan
I
Usov
Jae Sun
JS
Jeong
Francesco S
FS
Ruggeri
Giovanni
G
Dietler
Hilal A
HA
Lashuel
Ian W
IW
Hamley
Raffaele
R
Mezzenga
D000682:Amyloid / Q000737:chemistry* / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002417:Cattle; D055119:Elastic Modulus; D007782:Lactoglobulins / Q000737:chemistry / Q000378:metabolism; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology / Q000379:methods*; D010047:Ovalbumin / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D051844:alpha-Synuclein / Q000737:chemistry / Q000378:metabolism; D016875:tau Proteins / Q000737:chemistry / Q000378:metabolism
D000682:Amyloid; D016229:Amyloid beta-Peptides; D007782:Lactoglobulins; D010446:Peptide Fragments; D051844:alpha-Synuclein; C075222:amyloid beta-protein (1-42); D016875:tau Proteins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D009113:Muramidase