Vps18 deficiency inhibits dendritogenesis in Purkinje cells by blocking the lysosomal degradation of Lysyl Oxidase.
Dendrite development occupies a central position in the formation of nervous system. However, whether lysosomal degradative function is required for dendritogenesis of neurons remains unknown. We have recently demonstrated the critical role of Vps18 in the lysosomal degradation pathway in mice. Here, we report that Vps18 deficiency severely blocks the dendrite development of Pukinje cells but not cerebral cortical neurons. Furthermore, we also demonstrate that the lysyl oxidase (Lox) protein is degraded through lysosome and accumulated in the Vps18 deficient cerebellum but not in cerebral cortices. Our results suggest that lysosome regulates dendritogenesis of Purkinje cells though degrading Lox.
Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai 200433, China.
Chao
C
Peng
Shunfei
S
Yan
Jian
J
Ye
Lingxi
L
Shen
Tian
T
Xu
Wufan
W
Tao
D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology; D003712:Dendrites / Q000201:enzymology / Q000502:physiology*; D008247:Lysosomes / Q000201:enzymology; D051379:Mice; D018345:Mice, Knockout; D008249:Protein-Lysine 6-Oxidase / Q000378:metabolism*; D059748:Proteolysis; D011689:Purkinje Cells / Q000201:enzymology / Q000502:physiology*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000502:physiology*
D033921:Vesicular Transport Proteins; C575743:Vps18 protein, mouse; D008249:Protein-Lysine 6-Oxidase