Anti-inflammatory effect of 3,4-DHPEA-EDA [2-(3,4 -hydroxyphenyl) ethyl (3S, 4E)-4-formyl-3-(2-oxoethyl)hex-4-enoate] on primary human vascular endothelial cells.
Diets in which fat is significantly provided by olive oil and are relatively rich in vegetables, have been associated with a low incidence of cardiovascular diseases, mostly due to the presence of several phenolic compounds which have anti-oxidant and antiinflammatory properties. [1]. In this work, we describe the anti-inflammatory effect of 3,4-DHPEA-EDA in a cell model that we developed to mimic inflammatory injury of endothelium. This was based on the production of the proinflammatory chemokine CCL2, following in vitro stimulation of primary human endothelial cells. Pre-treatment of cells with 3,4-DHPEA-EDA resulted in a dose-dependent inhibition of CCL2 secretion. The effect of 3,4-DHPEA-EDA on CCL2 expression was observed at the transcriptional level. Functional data have shown that 3,4-DHPEA-EDA diminished monocyte adhesion to HUVECs. These results point on the use of 3,4- DHPEA-EDA as a novel drug aimed to prevent or reduce inflammation of endothelium.
Department of Chemistry University of Calabria Ponte Bucci, cubo 12C, 87036 Arcavacata di Rende (CS) Italy. sindona@unical.it
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Sindona
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Caruso
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Cozza
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Fiorentini
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Lorusso
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Marini
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Nardi
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Procopio
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Zicari
D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D011714:Pyrans / Q000737:chemistry / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
C547883:3,4-dihydroxyphenylethanol-elenolic acid dialdehyde; D000893:Anti-Inflammatory Agents; D015815:Cell Adhesion Molecules; D018932:Chemokine CCL2; D008070:Lipopolysaccharides; D010636:Phenols; D011714:Pyrans; D014409:Tumor Necrosis Factor-alpha