Increased exposure of vitamin A by Chrysanthemum morifolium Ramat extract in rat was not via induction of CYP1A1, CYP1A2, and CYP2B1.
The aim of this study was to investigate the effect of Chrysanthemum morifolium Ramat (CM) extract on the pharmacokinetics of retinol and activities of cytochrome P450s (CYP450s) related to retinoid metabolism. Rats were treated with CM extract for 15 d. Plasma concentrations of retinol were measured following oral administration of retinol (45 mg/kg). Basal levels of retinol and retinoic acid in serum and liver were also measured. 7-Ethoxyresorufin-O-deethylase activity, phenacetin-O-deethylase activity, and 7-pentoxyresorufin-O-deethylase activities were used to assay the activities of CYP1A1, CYP1A2, and CYP2B1 in hepatic microsomes of rats, respectively. Protein expressions of the 3 CYP450s were measured by western blot. Our studies demonstrated that CM extract dose-dependently increased basal level of retinol in serum. In pharmacokinetic experiment, CM extract dose-dependently increased plasma concentrations of retinol after oral administration of retinol to rats treated with CM extract. But activities and expressions of CYP1A1, CYP1A2, and CYP2B1 in hepatic microsomes of rats were also induced by CM extract.
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical Univ, Nanjing, PR China.
Ping
P
Wang
Xian
X
Pan
Guanming
G
Chen
Jia
J
Li
Li
L
Liu
Xiaodong
X
Liu
Shi
S
Jin
Lin
L
Xie
Guangji
G
Wang
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000032:analysis; D031202:Chrysanthemum / Q000737:chemistry*; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis* / Q000378:metabolism; D019388:Cytochrome P-450 CYP1A2; D019362:Cytochrome P-450 CYP2B1 / Q000096:biosynthesis* / Q000378:metabolism; D003580:Cytochromes / Q000096:biosynthesis* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005419:Flavonoids / Q000032:analysis; D035264:Flowers / Q000737:chemistry; D007700:Kinetics; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014212:Tretinoin / Q000097:blood / Q000378:metabolism; D014801:Vitamin A / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D003580:Cytochromes; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D014801:Vitamin A; D002726:Chlorogenic Acid; D014212:Tretinoin; C017170:Cyp1a2 protein, rat; D019363:Cytochrome P-450 CYP1A1; D019388:Cytochrome P-450 CYP1A2; D019362:Cytochrome P-450 CYP2B1