[Investigation of tomato (Solanum lycopersicum) aminotransferases involved in biosynthesis of branched-chain-amino-acids].
This study presents evidence for the role of BCAT3 and BCAT4 proteins in the synthesis of branched-chain-amino-acids in tomato Solanum lycopersicum. BCAT3 and BCAT4 genes were located on tomato chromosomal map by RFLP method (restriction fragment length polymorphism). Using confocal microscopy it was shown that BCAT3-GFP and BCAT4-GFP fusion proteins were localised in chloroplasts. It was observed that these aminotransferase isoforms exhibited distinct kinetic properties and a differential expression pattern of mRNA levels in various tomato tissues.
A S
AS
Kochevenko
A R
AR
Fernie
D000597:Amino Acids, Branched-Chain / Q000096:biosynthesis*; D002736:Chloroplasts / Q000201:enzymology* / Q000235:genetics; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000737:chemistry / Q000235:genetics; D007527:Isoenzymes; D007700:Kinetics; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics; D018613:Microscopy, Confocal; D009928:Organ Specificity; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D000637:Transaminases / Q000235:genetics / Q000378:metabolism*
D000597:Amino Acids, Branched-Chain; D007527:Isoenzymes; D010940:Plant Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D000637:Transaminases