High-accuracy analysis system for the redox status of coenzyme Q10 by online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry.
The accurate profiling of oxidative stress markers is often disturbed by oxidation occurred during sample preparation. In this study, an online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry system was constructed utilizing supercritical fluid as an efficient solvent for extraction. A higher ratio of the reduced form of coenzyme Q(10) (CoQ(10)) was detected by online extraction, rather than by offline extraction using a conventional organic solvent. The results suggest that the profile of easily oxidized compounds can be acquired with high accuracy using this system because the oxidation of the target compounds during sample preparation can be avoided when using the online extraction.
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Atsuki
A
Matsubara
Kazuo
K
Harada
Kazumasa
K
Hirata
Eiichiro
E
Fukusaki
Takeshi
T
Bamba
D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D057075:Enzyme Assays / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D010084:Oxidation-Reduction; D012230:Rhizobiaceae / Q000737:chemistry / Q000201:enzymology; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
D014451:Ubiquinone; C024989:coenzyme Q10