Diagnosis of human parechovirus infections of the central nervous system with a commercial real-time reverse transcription-polymerase chain reaction kit and direct genotyping of cerebrospinal fluid specimens.
We screened 100 cerebrospinal fluid specimens for the human parechoviruses (HPeV) genome with the commercial parechovirus r-gene™ kit, which allows results to be available in a clinically relevant time frame. HPeV infection was diagnosed in 4 infants (<4 months) and all genotyped viruses were HPeV3.
CHU Clermont-Ferrand, Laboratoire de Virologie, Centre de Biologie, F-63003 Clermont-Ferrand, France. amirand@chu-clermontferrand.fr
Audrey
A
Mirand
Christine
C
Archimbaud
Martine
M
Chambon
Christel
C
Regagnon
Amélie
A
Brebion
Jean-Luc
JL
Bailly
Hélène
H
Peigue-Lafeuille
Cécile
C
Henquell
D000293:Adolescent; D000328:Adult; D000368:Aged; D002555:Cerebrospinal Fluid / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D018792:Encephalitis, Viral / Q000175:diagnosis* / Q000821:virology; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D030061:Parechovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000175:diagnosis* / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014773:Virology / Q000379:methods; D055815:Young Adult