A new strain of tomato severe leaf curl virus and a unique variant of tomato yellow leaf curl virus from Mexico.
The complete genome sequence of a distinct variant of tomato yellow leaf curl virus-Israel (TYLCV-IL) and the DNA-A sequence of a new strain of tomato severe leaf curl virus (ToSLCV) isolated in San Luis Potosi, Mexico, are described and analyzed. The TYLCV-IL[MX:SLP:11] variant differs from all TYLCV-IL isolates described so far by a unique 42-nt duplicated sequence comprising a part of the conserved stem-loop element of the virion-strand replication origin and adjacent regulatory sequences. TYLCV-IL[MX:SLP:11] was associated with tomato chino La Paz virus (ToChLPV-B[MX:SLP:11]) in a Solanum pimpinellifolium plant, and with pepper huasteco yellow vein virus (PHYVV-[MX:SLP:11]) and ToSLCV-GT[MX:SLP:11] in a Solanum lycopersicum plant. In addition, a distinct ToSLCV exhibiting low sequence identity (<89 %) to other ToSLCV isolates from Mexico was found in a tomato plant collected in the same field. Sequence analysis of this new ToSLCV strain indicates that it is a recombinant of close relatives of ToSLCV-GT[MX:SLP:11] and ToChLPV-B[MX:SLP:11] found in mixed infections with TYLCV-IL[MX:SLP:11].
Instituto Potosino de Investigación Científica y Tecnológica (IPICYT), A.C., Camino a la Presa de San José, 78216 San Luis Potosi, SLP, Mexico.
B
B
Bañuelos-Hernández
J A
JA
Mauricio-Castillo
Y
Y
Cardenas-Conejo
R G
RG
Guevara-González
G R
GR
Arguello-Astorga
D053839:Begomovirus / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D018551:Solanum lycopersicum / Q000821:virology*; D008800:Mexico; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004279:DNA, Viral