Establishment of a cell line persistently infected with chronic wasting disease prions.
Elk prion protein (PrP(C)) has been confirmed to be capable of rendering rabbit epithelial RK13 cells permissive to temporal infection by chronic wasting disease (CWD) prions. The present study satisfactorily generated persistently CWD prion-affected RK13 cells (RKC1-11) using elk PrP(C) expressing cells (elkRK13) that were generated via the lentiviral expression system with high efficiency. The elkRK13 cells have been shown to be permissive to accumulation of abnormal isoforms of prion protein (PrP(Sc)) resulting from CWD prions up to 97 serial passages thus far. This novel prion-affected cell line will help facilitate investigation of the molecular basis of CWD prion pathogenesis and confirmation of CWD prion infectivity in vitro.
Foreign Animal Disease Division, Animal Plant and Fisheries Quarantine and Inspection Agency, Anyang, Gyeonggi-do, Korea.
Hyo-Jin
HJ
Kim
Dong-Seob
DS
Tark
Yoon-Hee
YH
Lee
Min-Jeong
MJ
Kim
Won-Yong
WY
Lee
In-Soo
IS
Cho
Hyun-Joo
HJ
Sohn
Takashi
T
Yokoyama
D000818:Animals; D002460:Cell Line; D003670:Deer / Q000378:metabolism*; D005822:Genetic Vectors / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism*; D011328:Prions / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D056910:Republic of Korea; D034081:Wasting Disease, Chronic / Q000378:metabolism*
D011328:Prions