Live cell imaging of G protein-coupled receptors.
Live cell imaging experiments with G protein-coupled receptors (GPCRs) tagged with fluorescent fusion proteins were originally performed to study trafficking and subcellular location of these important drug targets. In the past decade, however, substantial progress came from improved imaging methods and from the cloning of novel fluorescent fusion proteins. Today, these methods allow to visualize not only GPCR interactions but also, e.g., receptor activation, trafficking between subcellular compartments, and to measure transport kinetics. Here, we summarize recent progress in live cell imaging of GPCRs using a confocal laser scanning microscope.
Leibniz-Institut für Molekulare Pharmakologie, Berlin, Germany.
Anke
A
Teichmann
Antje
A
Schmidt
Burkhard
B
Wiesner
Alexander
A
Oksche
Ralf
R
Schülein
D002470:Cell Survival; D057809:HEK293 Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D043562:Receptors, G-Protein-Coupled