Inhibition of UVB-induced wrinkle formation and MMP-9 expression by mangiferin isolated from Anemarrhena asphodeloides.
Chronic exposure of human skin to solar ultraviolet (UV) radiation causes photoaging. Naturally occurring phytochemicals are known to have anti-photoaging effects. The present study examined the effect of mangiferin isolated from Anemarrhena asphodeloides on wrinkle formation, skin thickness, and changes in collagen fibers in hairless mice. The in vitro effects and possible mechanism of mangiferin on UVB irradiation were determined in human keratinocyte (HEKa) cells. In vitro results showed that mangiferin reduced UVB-induced matrix metalloproteinase (MMP)-9 protein expression and enzyme activity and subsequent attenuation of UVB-induced phosphorylation of mitogen-activated protein kinase kinase1 (MEK) and extracellular signal-regulated kinase (ERK). In the in vivo studies, mangiferin inhibited UVB-induced mean length and mean depth of skin wrinkle based on skin replica, epidermal thickening, and damage to collagen fiber. Taken together, these results indicate that mangiferin exerts anti-photoaging activity in UVB-irradiated hairless mice by regulating MMP-9 expression through inhibition of MEK and ERK.
Biomolecular Science, University of Science and Technology, Daejon 305-806, Republic of Korea.
Hui-Seong
HS
Kim
Jae Hyoung
JH
Song
Ui Joung
UJ
Youn
Jin Won
JW
Hyun
Woo Seok
WS
Jeong
Mi Young
MY
Lee
Hwa Jung
HJ
Choi
Hyeong-Kyu
HK
Lee
Sungwook
S
Chae
D031403:Anemarrhena; D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D057809:HEK293 Cells; D006801:Humans; D015603:Keratinocytes; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008812:Mice, Hairless; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D027343:Rhizome; D015595:Skin Aging / Q000187:drug effects* / Q000473:pathology; D014466:Ultraviolet Rays / Q000009:adverse effects; D044004:Xanthones / Q000302:isolation & purification / Q000494:pharmacology*
D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D044004:Xanthones; C013592:mangiferin; D020780:Matrix Metalloproteinase 9