[Full sequence analysis for a null allele of MICA gene (MICA*063N)].
OBJECTIVE
To analyze the full nucleotide sequence of a null allele of major histocompatibility complex class I chain-related gene (MICA).


METHODS
A sequence-based typing method was used to determine the nucleotide sequence of the MICA gene. Potential alleles were identified with a computer program.


RESULTS
The identified allele has possessed a sequence similar to that of MICA*027 except for a C→T substitution at position 184 in codon 62 (CAG→TAG) of exon 2. As a stop codon, this may result in a truncated protein.


CONCLUSIONS
A null allele of MICA gene has been identified. The sequence has been submitted to the Genbank nucleotide sequence database (submission No. HWS10011131), which was officially named as MICA*063N by the WHO Nomenclature Committee in October 2010.
Department of Surgery, Guangzhou First Municipal People's Hospital Affiliated to Guangzhou Medical College, Guangzhou, Guangdong 510180, P.R.China. huangyingfeng@yahoo.com.cn
Ying-feng
YF
Huang
Yin
Y
Tan
Shao-yu
SY
Yang
Hong-tu
HT
Luo
Tai-chen
TC
Zou
D000483:Alleles; D001483:Base Sequence; D018388:Codon, Terminator; D005091:Exons; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D017421:Sequence Analysis / Q000379:methods
D018388:Codon, Terminator; D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A