Paenibacillus thermophilus sp. nov., a novel bacterium isolated from a sediment of hot spring in Fujian province, China.
A Gram-positive, thermophilic, strictly aerobic bacterium, designated WP-1(T), was isolated from a sediment sample from a hot spring in Fujian province of China and subjected to a polyphasic taxonomic study. Cells of strain WP-1(T) were rods (~0.6-0.8 × 2.5-3.5 μm) and motile by means of peritrichous flagella. Endospores were ellipsoidal in terminal or subterminal positions. Strain WP-1(T) grew at 37-60 °C (optimum 42-45 °C), 0-3 % NaCl (optimum 1 %, w/v) and pH 3.0-9.0 (optimum pH 6.5-7.0). The predominant menaquinone was MK-7. The major fatty acids were anteiso-C(15:0), iso-C(16:0), C(16:0) and anteiso-C(17:0). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two glycolipids, two unidentified phospholipids and two unknown polar lipids. The cell-wall peptidoglycan contained meso-diaminopimelic acid (meso-DAP). The G + C content of the genomic DNA was 52.5 %. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain WP-1(T) is a member of the genus Paenibacillus and exhibited sequence similarity of 99.3 % to Paenibacillus macerans DSM 24(T) and both strains represented a separate lineage from all other Paenibacillus species. However, the level of DNA-DNA relatedness between strain WP-1(T) and P. macerans DSM 24(T) was 34.0 ± 4.7 %. On the basis of phylogenetic, physiological and chemotaxonomic analysis data, strain WP-1(T) is considered to represent as a novel species of the genus Paenibacillus, for which the name Paenibacillus thermophilus sp. nov., is proposed, with the type strain WP-1(T) (=DSM 24746(T) = JCM 17693(T) = CCTCC AB 2011115(T)).
Zhejiang Province Key Laboratory for Food Safety, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Yu
Y
Zhou
Sheng
S
Gao
Da-Qiao
DQ
Wei
Ling-Ling
LL
Yang
Xing
X
Huang
Jie
J
He
Yun-Jiao
YJ
Zhang
Shu-Kun
SK
Tang
Wen-Jun
WJ
Li
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella / Q000502:physiology; D019015:Geologic Sediments / Q000382:microbiology*; D045482:Hot Springs; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride; D003960:Diaminopimelic Acid