Oligodendrocytes enforce immune tolerance of the uninfected brain by purging the peripheral repertoire of autoreactive CD8+ T cells.
Myelin-specific CD8(+) T cells are thought to contribute to the pathogenesis of multiple sclerosis. Here we modeled this contribution in mice with CD8(+) T cells recognizing ovalbumin (OVA) expressed in oligodendrocytes (ODCs). Surprisingly, even very high numbers of activated OVA-reactive CD8(+) T cells failed to induce disease and were cleared from the immune system after antigen encounter in the central nervous system (CNS). Peripheral infection with OVA-expressing Listeria (Lm-OVA) enabled CD8(+) T cells to enter the CNS, leading to the deletion of OVA-specific clones after OVA recognition on ODCs. In contrast, intracerebral infection allowed OVA-reactive CD8(+) T cells to cause demyelinating disease. Thus, in response to infection, CD8(+) T cells also patrol the CNS. If the CNS itself is infected, they destroy ODCs upon cognate antigen recognition in pursuit of pathogen eradication. In the sterile brain, however, antigen recognition on ODCs results in their deletion, thereby maintaining tolerance.
Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany.
Shin-Young
SY
Na
Andreas
A
Hermann
Monica
M
Sanchez-Ruiz
Alexander
A
Storch
Martina
M
Deckert
Thomas
T
Hünig
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017209:Apoptosis / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D015551:Autoimmunity; D001921:Brain / Q000276:immunology* / Q000382:microbiology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D006410:Hematopoiesis / Q000276:immunology; D007108:Immune Tolerance; D039041:Integrin alpha4beta1 / Q000276:immunology; D008089:Listeria monocytogenes / Q000276:immunology; D008088:Listeriosis / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009836:Oligodendroglia / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D010047:Ovalbumin / Q000276:immunology; D017154:Stromal Cells / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology; D019014:fas Receptor / Q000276:immunology
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D039041:Integrin alpha4beta1; D019014:fas Receptor; D010047:Ovalbumin