Paramagnetic 19F chemical shift probes that respond selectively to calcium or citrate levels and signal ester hydrolysis.
Paramagnetic magnetic resonance chemical shift probes containing a proximal CF(3) group have been characterised. Different systems have been created that report reversible changes in calcium ion concentrations in the millimolar regime, signal the presence of citrate selectively in competitive aqueous media and allow the monitoring of remote ester/amide hydrolysis in relayed, irreversible transformations. Chemical shift non-equivalence is amplified by the presence of the proximate lanthanide ion, with a mean separation between the CF(3) group and the metal ion of 6.4 Å found for a thulium complex, in an X-ray structure of the metal complex aqua adduct. The enhanced rate of longitudinal relaxation of the (19)F nucleus allows faster data acquisition.
Department of Chemistry, Durham University, South Road, Durham DH1 3LE, UK.
Peter
P
Harvey
Kirsten H
KH
Chalmers
Elena
E
De Luca
Anurag
A
Mishra
David
D
Parker