Interleukin-6 inhibits L-type calcium channel activity of cultured cerebellar granule neurons.
Our previous work has shown that interleukin-6 (IL-6) implements its neuroprotective effect by inhibiting the intracellular Ca(2+) overload in neurons. Here, we examined whether regulation of L-type calcium channels (LCCs) activities is involved in the neuroprotective action of IL-6. In cultured cerebellar granule neurons (CGNs), patch-clamp recording showed that the whole-cell Ca(2+) current and LCC current were significantly reduced by IL-6 pretreatment (120 ng/ml, for 24 h). Calcium imaging data indicated that IL-6 significantly suppressed high K(+)-induced intracellular Ca(2+) overload and LCC Ca(2+) influx. Moreover, expression of the LCC subunit, Ca(v)1.2, was remarkably downregulated by IL-6 in cultured CGNs. These findings suggest that IL-6 exerts a neurotrophic effect by preventing Ca(2+) overload, at least partly through inhibition of LCC activity in cultured CGNs.
Department of Anatomy and Histology & Embryology, School of Biology and Basic Medical Sciences, Soochow University, 1 Shizi Street, Suzhou, 215006, China.
Song-Hua
SH
Ma
Bing
B
Li
Hui-Wei
HW
Huang
Yu-Ping
YP
Peng
Yi-Hua
YH
Qiu
D000818:Animals; D000831:Animals, Newborn; D020746:Calcium Channels, L-Type / Q000096:biosynthesis*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects*; D019610:Cytoprotection; D015850:Interleukin-6 / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology
D020746:Calcium Channels, L-Type; D015850:Interleukin-6; D011994:Recombinant Proteins; D011188:Potassium