Kangiella sediminilitoris sp. nov., isolated from a tidal flat sediment.
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped bacterial strain, BB-Mw22(T), was isolated from a tidal flat sediment of the South Sea in South Korea. It grew optimally at 30-37 °C, at pH 7.0-7.5 and in the presence of 2-3 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain BB-Mw22(T) belonged to the genus Kangiella and the cluster comprising Kangiella species and strain BB-Mw22(T) was clearly separated from other taxa. Strain BB-Mw22(T) exhibited 95.3-98.7 % 16S rRNA gene sequence similarity to the type strains of recognized Kangiella species. Strain BB-Mw22(T) contained Q-8 as the predominant ubiquionone and iso-C15 : 0 and iso-C11 : 0 3-OH as the major fatty acids. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and one unidentified aminolipid. The DNA G+C content of strain BB-Mw22(T) was 48.9 mol%, and its mean DNA-DNA hybridization values with Kangiella geojedonensis YCS-5(T), Kangiella japonica JCM 16211(T) and Kangiella taiwanensis JCM 17727(T) were 14-28 %. Phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain BB-Mw22(T) is distinguishable from all recognized Kangiella species. On the basis of the data presented, strain BB-Mw22(T) is considered to represent a novel species of the genus Kangiella, for which the name Kangiella sediminilitoris sp. nov. is proposed. The type strain is BB-Mw22(T) ( = KCTC 23892(T)  = CCUG 62217(T)).
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 306-809, Republic of Korea.
Soo-Young
SY
Lee
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 306-809, Republic of Korea.
Sooyeon
S
Park
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 306-809, Republic of Korea.
Tae-Kwang
TK
Oh
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea.
Jung-Hoon
JH
Yoon
D053521:Alcanivoraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8