Glandular trichomes of Ceratotheca triloba (Pedaliaceae): morphology, histochemistry and ultrastructure.
This study was initiated to characterize the distribution, morphology, secretion mode, histochemistry and ultrastructure of the glandular trichomes of Ceratotheca triloba using light and electron microscopy. Its leaves bear two morphologically distinct glandular trichomes. The first type has long trichome with 8-12 basal cells of pedestal, 3-14 stalk cells, a neck cell and a head of four cells in one layer. The second type has short trichome comprising one or two basal epidermal cells, a unicellular or bicellular stalk and a multicellular head of two to eight cells. There is a marked circular area in the upper part of each head cell of the long trichome. This area is provided with micropores to exudate directly the secretory product onto the leaf surface by an eccrine pathway. The secretory product has copious amount of dark microbodies arising from plastids which are positive to Sudan tests and osmium tetroxide for unsaturated lipids. The secretion mode of short trichomes is granulocrine and involves two morphologically and histochemically distinct vesicle types: small Golgi-derived vesicles which are positive to Ruthenium Red test for mucilaginous polysaccharides; the second type is dark large microbodies similar to that of long trichomes with low quantity. These two types are stored in numerous peripheral vacuoles and discharge their contents accompanied by the formation of irregular invaginations of the plasmalemma inside the vacuoles via reverse pinocytosis. These two secretion modes of long and short trichomes are reported for the first time in the family Pedaliaceae. The long trichomes have more unsaturated lipids, while the short trichomes contain more mucilaginous polysaccharides.
School of Life Sciences, University of KwaZulu-Natal, P/Bag X54001, Durban 4000, South Africa. naidooy1@ukzn.ac.za
Yougasphree
Y
Naidoo
Taariq
T
Karim
Samia
S
Heneidak
Channangihalli Thimmegowda
CT
Sadashiva
Gonasageran
G
Naidoo
D000470:Alkaloids / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D006651:Histocytochemistry; D008055:Lipids / Q000032:analysis; D008830:Microbodies / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D029601:Pedaliaceae / Q000737:chemistry / Q000648:ultrastructure*; D010636:Phenols / Q000032:analysis; D010873:Pinocytosis; D019441:Plant Epidermis / Q000737:chemistry / Q000648:ultrastructure*; D053147:Plant Exudates / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry / Q000648:ultrastructure; D011134:Polysaccharides / Q000032:analysis
D000470:Alkaloids; D005419:Flavonoids; D008055:Lipids; D010636:Phenols; D053147:Plant Exudates; D011134:Polysaccharides