Cell surface shaving of Candida albicans biofilms, hyphae, and yeast form cells.
We used a brief trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS to identify the surface proteins on live Candida albicans organisms growing in biofilms and planktonic yeast cells and hyphae. One hundred thirty-one proteins were present in at least two of the three replicates of one condition and distributed in various combinations of the three growth conditions. Both previously reported and new surface proteins were identified and these were distributed between covalently attached proteins and noncovalently attached proteins of the cell wall.
Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid and Ramón y Cajal Institute of Health Research, Madrid, Spain.
Vital
V
Vialás
Palani
P
Perumal
Dolores
D
Gutierrez
Pilar
P
Ximénez-Embún
César
C
Nombela
Concha
C
Gil
W LaJean
WL
Chaffin
D018441:Biofilms; D002176:Candida albicans / Q000737:chemistry / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D025301:Hyphae / Q000737:chemistry / Q000166:cytology; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods
D005656:Fungal Proteins; D010446:Peptide Fragments