[Study of dahuangzhechong pills on anti-arterial thrombosis with the orthogonal design].
OBJECTIVE
To screen the main component of Dahuangzhechong pill's anti-arterial thrombosis with the orthogonal design and refine Dahuangzhechong pills.


METHODS
In accordance with the orthogonal design table (L(16)2(15)), divided herbs into 16 groups and made the appropriate liquid. The liquid was gave to SD rats by intragastric administration,the model group, normal control group received the same volume of physiological saline. Isolated rats' carotid artery after intragastric administration a week,modeled according to ferric chloride inducement the carotid artery thrombosis method, then collected blood, detected content of platelet, thromboxane B2 (TXB2) and 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha), sheared and measured dry weight of the modeling artery, then placed arteries in 10% formalin fixation, observed morphological changes in vascular tissue by HE staining.


RESULTS
Pathological examination revealed: each experimental group had thrombosis, softening, dissolution, absorption, and intimal injury, but the severity of thrombosis were diferent. Orthogonal analysis showed: 1, influence on dry weight of thrombus: rhubarb, ground beetle, leeches, peach seed, dry paint, except dry paint P<0.05, the others P<0.01.2, influence on plasma 6- keto-PGF1alpha level: peach seed, dry paint, ground beetle, gadfly, grubs, leeches, rhubarb, except rhubarb P<0.05, the others P<0.01.3, influence on plasma TXB2: ground beetle, peach seed, dried paint, rhubarb, leeches, except leech P<0.05, the others P<0.01.4, influence on platelet count: peach seed, dry paint, rhubarb, ground beetle, gadfly, leeches, except gadfly, leeches P<0.05, the others P<0.01.


CONCLUSIONS
Anti-artery thrombosis of Dahuangzhechong Pill is most closely related with rhubarb, ground beetle, leeches, peach seed, dry paint and gadfly.
Xiangya Hospital Central South University, Changsha 410008, China.
Xing
X
Wei
Zhan-Yi
ZY
Wang
Jie-Yang
JY
Li
Ling
L
Wan
Fu-Yuan
FY
He
Xiao-Peng
XP
Duan
Guang-Wei
GW
Zhong
Yao
Y
Jiang
Dong-Sheng
DS
Wang
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D002341:Carotid Artery Thrombosis / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D017536:Carotid Artery, Common; D004195:Disease Models, Animal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012250:Rheum; D015912:Thrombolytic Therapy; D013929:Thromboxane B2 / Q000097:blood
C521634:Dahuang Zhechong pill; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005343:Fibrinolytic Agents; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha