The primary use in indirect ELISA of secreted proteins Mb1761c and Mb2277 of M. bovis.
Disease monitoring on bovine tuberculosis (BTB) has been proven to be a capable strategy to control this disease, which has significantly progressed in developed countries. However, such strategies require a prompt, accurate live animal test, which has thus far been lacking in developing countries, such as China. The two secreted proteins, Mb1761c and Mb2277, were selected from the complete genome of M. bovis by using a bioinformatics method. After having been proven by the reactogenicity by Western blot, the two expressed prokaryotic proteins served as coat antigens in ELISA plates to evaluate their ability to detect the antibodies against M. bovis. The results showed that the sensitivity of the indirect ELISA method coated with Mb2277 as antigen was as same as that coated with Mb1761c. To explore the possibility of increasing the sensitivity of the ELISA test, the two proteins were further mixed in a different ratio and coated as antigen in ELISA. The results showed that the sensitivity of the combination of the coated reconstructive proteins (1:1) was stronger than that of single coated protein.
Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, China.
Fang
F
Liu
Yuan
Y
Lin
Benqiang
B
Li
Man
M
Wang
Jianguo
J
Zhu
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D009163:Mycobacterium bovis / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins