Golgi complex fragmentation in G2/M transition: An organelle-based cell-cycle checkpoint.
In mammalian cells, the Golgi complex is organized into a continuous membranous system known as the Golgi ribbon, which is formed by individual Golgi stacks that are laterally connected by tubular bridges. During mitosis, the Golgi ribbon undergoes extensive fragmentation through a multistage process that is required for its correct partitioning into the daughter cells. Importantly, inhibition of this Golgi disassembly results in cell-cycle arrest at the G2 stage, suggesting that accurate inheritance of the Golgi complex is monitored by a "Golgi mitotic checkpoint." Here, we discuss the mechanisms and regulation of the Golgi ribbon breakdown and briefly comment on how Golgi partitioning may inhibit G2/M transition.
Institute of Protein Biochemistry, National Research Council (CNR), Via Pietro Castellino 111, Naples, Italy. d.corda@ibp.cnr.it
Daniela
D
Corda
Maria Luisa
ML
Barretta
Romina Ines
RI
Cervigni
Antonino
A
Colanzi
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D016195:G2 Phase / Q000502:physiology*; D006056:Golgi Apparatus / Q000502:physiology*; D000077614:Golgi Matrix Proteins; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008938:Mitosis / Q000502:physiology*; D051381:Rats; D012097:Repressor Proteins / Q000378:metabolism
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C108705:GORASP1 protein, human; C122048:GORASP2 protein, human; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; D012097:Repressor Proteins; D000429:Alcohol Oxidoreductases; C096794:C-terminal binding protein