[Analysis of microbial diversity in rectum of calf in large-scale cattle farm].
OBJECTIVE
To analyze the diversity of bacterial community in rectum of diarrheic calves, and differences with health calves.


METHODS
16S rRNA clone libraries were constructed, positive clones were digested by Msp I and Hha I for restriction fragment length polymorphism (RFLP), and then a phylogenetic tree was depicted based on the 16S rRNA sequencing, to confirm the compose of microbe in the diarrheic calf rectum.


RESULTS
The positive rate of clone was 98.75% (474/480) in diarrheic calves, the dominant bacteria included Lactobacillus (14%), Enterococcus (10%) and Escherichia (8%). The positive rate of clone was 96.45% (488/506) in health samples, the dominant bacteria included Clostridium (13%), Bifidobacterium (8%), Megasphaera (5%).


CONCLUSIONS
Complexity and diversity of bacterial community in rectum in 2 weeks old calves had their own features, and significant increase of Lactobacillus, Enterococcus and Escherichia was found in diarrhea calves.
The Breed and Biotechnology Key Laboratory of Sheep in Reproduction and Construction Group of Xinjiang, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China. cszero6130@163.com
Qiang
Q
Xu
Lichao
L
Kang
Xinwen
X
Bo
Xun
X
Ma
D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012007:Rectum / Q000382:microbiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S