Light scattering: a novel approach to analyze protein 4.1R FERM domain interaction with inside-out-vesicles in solution.
In this study, we describe a novel application for light scattering, a method widely used for separation of molecules in solution based on their size.  We demonstrate that light scattering analysis can monitor the change in particle size of protein 4.1R prior to and after binding to red blood cell inside-out-vesicles in solution.  Light scattering constitutes therefore a novel tool to analyze protein-binding association constants.
Sysmex Corporation, Kobe, Japan. Shiba.Kouhei@sysmex.co.jp
Kohei
K
Shiba
Wataru
W
Nunomura
Yuichi
Y
Takakuwa
D003598:Cytoskeletal Proteins / Q000032:analysis*; D004912:Erythrocytes / Q000737:chemistry; D008027:Light; D008565:Membrane Proteins / Q000032:analysis*; D010316:Particle Size; D012542:Scattering, Radiation; D012996:Solutions
D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D012996:Solutions; C028841:erythrocyte membrane band 4.1 protein