TRPA1 is a polyunsaturated fatty acid sensor in mammals.
Fatty acids can act as important signaling molecules regulating diverse physiological processes. Our understanding, however, of fatty acid signaling mechanisms and receptor targets remains incomplete. Here we show that Transient Receptor Potential Ankyrin 1 (TRPA1), a cation channel expressed in sensory neurons and gut tissues, functions as a sensor of polyunsaturated fatty acids (PUFAs) in vitro and in vivo. PUFAs, containing at least 18 carbon atoms and three unsaturated bonds, activate TRPA1 to excite primary sensory neurons and enteroendocrine cells. Moreover, behavioral aversion to PUFAs is absent in TRPA1-null mice. Further, sustained or repeated agonism with PUFAs leads to TRPA1 desensitization. PUFAs activate TRPA1 non-covalently and independently of known ligand binding domains located in the N-terminus and 5(th) transmembrane region. PUFA sensitivity is restricted to mammalian (rodent and human) TRPA1 channels, as the drosophila and zebrafish TRPA1 orthologs do not respond to DHA. We propose that PUFA-sensing by mammalian TRPA1 may regulate pain and gastrointestinal functions.
Department of Pharmacology and Physiology, Georgetown University, Washington, District of Columbia, USA.
Arianne L
AL
Motter
Gerard P
GP
Ahern
D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism; D004330:Drosophila; D019858:Enteroendocrine Cells / Q000187:drug effects / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D008322:Mammals / Q000235:genetics / Q000378:metabolism; D051379:Mice; D054730:Protein Interaction Domains and Motifs; D051381:Rats; D011984:Sensory Receptor Cells / Q000378:metabolism; D013649:Taste / Q000235:genetics; D050051:Transient Receptor Potential Channels / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish
D005231:Fatty Acids, Unsaturated; D050051:Transient Receptor Potential Channels; D002766:Cholecystokinin