Live Helicobacter pylori in the root canal of endodontic-infected deciduous teeth.
BACKGROUND
Many polymerase chain reaction (PCR)-based studies have shown that Helicobacter pylori DNA is prevalent in the oral cavity, but reports on the isolation of live bacteria are extremely rare. Thus, it is still unclear whether H. pylori can indeed survive in the oral environment.


METHODS
Here we used electron microscopy, selective growth techniques, urease assays, 16S rRNA PCR, and western blotting to investigate the possible presence of live H. pylori in 10 root canal and corresponding plaque samples of endodontic-infected deciduous teeth in three children.


RESULTS
Although H. pylori DNA was verifiable by PCR in several plaque and root canal samples, bacterial colonies could only be grown from two root canals, but not from plaque. These colonies were unequivocally identified as H. pylori by microscopic, genetic, and biochemical approaches.


CONCLUSIONS
Our findings show that root canals of endodontic-infected teeth may be a reservoir for live H. pylori that could serve as a potential source for transmission.
Department of Paediatric Dentistry, University School of Dental Medicine, University of Leipzig, Nuernberger Straße 57, 04103, Leipzig, Germany. Christian.Hirsch@medizin.uni-leipzig.de
Christian
C
Hirsch
Nicole
N
Tegtmeyer
Manfred
M
Rohde
Marion
M
Rowland
Omar A
OA
Oyarzabal
Steffen
S
Backert
D002675:Child, Preschool; D003731:Dental Caries / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D016133:Polymerase Chain Reaction; D014094:Tooth, Deciduous / Q000382:microbiology