MgFe-layered double hydroxide modified electrodes for direct electron transfer of heme proteins.
In this study, the Fe-based layered double hydroxides (Mg(3)Fe LDH) were used to immobilize heme proteins including hemoglobin (Hb), myoglobin (Mb) and horseradish peroxidase (HRP) for fabrication of heme/Mg(3)Fe LDH film on glassy carbon electrode (Mg(3)Fe-heme/GCE). The possible role of iron in framework of LDH to promote direct electron transfer (DET) of heme proteins was investigated using an LDH containing non-iron as a reference. Hb was selected as a model protein for studying the electrocatalytic activity of immobilized heme in LDH film. The Mg(3)Fe-Hb/GCE displayed an enhanced electrocatalytic reduction towards H(2)O(2). The biosensor showed a very low detection limit (0.036 μM) and apparent Michaelis-Menten constant (7.98 μM). This work outlines that Fe-based LDH modified electrode provides a promising platform for immobilization of heme proteins and development of sensitive biosensors.
Key Laboratory of Chemo-Biosensing, Anhui Province, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. limaoguo@mail.ahnu.edu.cn
Maoguo
M
Li
Huiqin
H
Ji
Yinling
Y
Wang
Lin
L
Liu
Feng
F
Gao
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002417:Cattle; D004566:Electrodes; D004579:Electron Transport; D006420:Hemeproteins / Q000737:chemistry* / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D006878:Hydroxides / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D058085:Iron Compounds / Q000737:chemistry*; D057230:Limit of Detection; D017616:Magnesium Compounds / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results
D006420:Hemeproteins; D006454:Hemoglobins; D006878:Hydroxides; D055767:Immobilized Proteins; D058085:Iron Compounds; D017616:Magnesium Compounds; D009211:Myoglobin; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase