Mixotrophic cultivation of Chlorella vulgaris using industrial dairy waste as organic carbon source.
Growth parameters and biochemical composition of the green microalga Chlorella vulgaris cultivated under different mixotrophic conditions were determined and compared to those obtained from a photoautotrophic control culture. Mixotrophic microalgae showed higher specific growth rate, final biomass concentration and productivities of lipids, starch and proteins than microalgae cultivated under photoautotrophic conditions. Moreover, supplementation of the inorganic culture medium with hydrolyzed cheese whey powder solution led to a significant improvement in microalgal biomass production and carbohydrate utilization when compared with the culture enriched with a mixture of pure glucose and galactose, due to the presence of growth promoting nutrients in cheese whey. Mixotrophic cultivation of C. vulgaris using the main dairy industry by-product could be considered a feasible alternative to reduce the costs of microalgal biomass production, since it does not require the addition of expensive carbohydrates to the culture medium.
IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Ana P
AP
Abreu
Bruno
B
Fernandes
António A
AA
Vicente
José
J
Teixeira
Giuliano
G
Dragone
D052818:Autotrophic Processes / Q000187:drug effects / Q000528:radiation effects; D002244:Carbon / Q000378:metabolism* / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D048408:Chlorella vulgaris / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D003612:Dairying; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007220:Industrial Waste / Q000032:analysis*; D008027:Light; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D009930:Organic Chemicals / Q000378:metabolism* / Q000494:pharmacology; D010860:Pigments, Biological / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D013213:Starch / Q000096:biosynthesis / Q000378:metabolism
D007220:Industrial Waste; D008055:Lipids; D009930:Organic Chemicals; D010860:Pigments, Biological; D011506:Proteins; D002244:Carbon; D013213:Starch; D005947:Glucose; D005690:Galactose