Enhancement of microalgal biomass and lipid productivities by a model of photoautotrophic culture with heterotrophic cells as seed.
For overcoming the long period of seed cultured photoautotrophically and inadequate cell supply for the inoculation of microalgae photoautotrophic cultivation, a model for the photoautotrophic culture of three Chlorella species with heterotrophic cells as seed was investigated. The model can not only take advantages of rapid cell growth in heterotrophic process for preparation of cells as seed but also increase the biomass and lipid productivities of the microalgae cultivated photoautotrophically. The results showed that biomass productivities of Chlorella pyrenoidosa, Chlorella ellipsoidea and Chlorella vulgaris cultured by heterotrophy were 20.9, 26.9 and 25.2 times higher than those by photoautotrophy in seed culturing period. In the subsequent photoautotrophic culture, the biomass and lipid productivities of C. pyrenoidosa, C. ellipsoidea and C. vulgaris with heterotrophic seed were 1.91, 1.51, 1.48 and 1.66, 1.37, 1.42 times higher than those with photoautotrophic seed. Furthermore, the culture model was also carried out successfully outdoor.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
Feifei
F
Han
Jianke
J
Huang
Yuanguang
Y
Li
Weiliang
W
Wang
Jun
J
Wang
Jianhua
J
Fan
Guomin
G
Shen
D052818:Autotrophic Processes / Q000187:drug effects / Q000528:radiation effects*; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects / Q000528:radiation effects; D018929:Cell Culture Techniques; D002708:Chlorella / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects; D002734:Chlorophyll / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D052836:Heterotrophic Processes / Q000187:drug effects / Q000528:radiation effects*; D008027:Light; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects; D008954:Models, Biological; D013997:Time Factors
D005227:Fatty Acids; D008055:Lipids; D002734:Chlorophyll; D005947:Glucose