Paradoxical effects of alcohol and thiamine deficiency on the eye opening in rat pups.
OBJECTIVE
The present study attempts to determine whether developmental thiamine (B1 vitamin) deficiency and developmental ethanol exposure disturb eye opening in Wistar rat pups.


METHODS
During gestation and lactation, Wistar rat dams were exposed to the following treatments: (1) Prenatal thiamine-deficient dams; (2) perinatal thiamine-deficient dams; (3) postnatal thiamine-deficient dams; (4) 12% alcohol/water drinking mothers; (5) mothers drinking 12% alcohol/water + thiamine hydrochloride mixture; (6) ad libitum control dams. Pair-feeding treatments controlled malnutrition related to thiamine deficiency: (7) Prenatal pair-fed dams; (8) perinatal pair-fed dams; (9) postnatal pair-fed dams and included also the control of alcohol consummation: (10) pair-fed saccharose dams. After birth, from postnatal day 10 (P10) to P18, eye opening was observed in the pups bred by ten different experimental dams.


RESULTS
The present experiments showed eye opening to be delayed strongly in perinatal thiamine-deficient pups only. Consequently, our study suggests perinatal thiamine deficiency to interfere with photoreceptors differentiation in the rat retina. In addition, our results reveal that developmental alcohol exposure-induced premature eye opening contrasted paradoxically with perinatal thiamine deficiency-induced delayed opening.


CONCLUSIONS
The results suggest differential actions of alcohol and thiamine deficiency on cellular genesis in the rat retina.
Université de Cocody, UFR Biosciences, Côte d'Ivoire. abdouba3000@hotmail.com
Abdoulaye
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D000428:Alcohol Drinking / Q000009:adverse effects; D000818:Animals; D000831:Animals, Newborn; D000431:Ethanol / Q000009:adverse effects / Q000494:pharmacology*; D005123:Eye / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development / Q000503:physiopathology; D005260:Female; D005325:Fetal Organ Maturity / Q000187:drug effects; D007774:Lactation / Q000187:drug effects; D009799:Ocular Physiological Phenomena / Q000187:drug effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013831:Thiamine / Q000494:pharmacology*; D013832:Thiamine Deficiency / Q000503:physiopathology*
D000431:Ethanol; D013831:Thiamine