Lymphocytic enzymes and lipid peroxidation in patients with metabolic syndrome.
OBJECTIVE
Metabolic syndrome (MetS) is considered a state of chronic inflammation. This study aimed to ascertain selected parameters of purinergic and cholinergic systems related to glucose metabolism and inflammation, as well as (γ)-glutamyltransferase (GGT) and N-acetyl-b-glucosaminidase (NAG) activities and lipoperoxidation in lymphocytes of patients with MetS.


METHODS
The adenosine deaminase (ADA), dipeptidyl peptidase IV (DPP-IV), acetylcholinesterase (AChE), GGT and NAG activities, as well as thiobarbituric acid reactive substances (TBARS) levels were investigated in lymphocytes of patients with MetS (n=38) and healthy volunteers (n=41). We also evaluated the insulin levels, anthropometric measurements and routine biochemical analyses.


RESULTS
ADA (p<0.05), DPP-IV and AChE (p<0.0001) activities were higher in patients with MetS when compared to the control group. Furthermore, we observed correlations between ADA and DPP-IV activities (p=0.0002; r=0.5945), TBARS levels and ADA (p=0.0021; r=0.5172) and DPP-IV activities (p=0.0022; r=0.5010).


CONCLUSIONS
Our findings showed that MetS might cause tissue distress that disturbed lymphocytic ADA, DPP-IV and AChE activities in response to inflammatory stimuli. These alterations evidence clinical abnormalities, since these enzymatic systems are able to regulate several aspects of adipose tissue function and inflammatory state of MetS and could be used successfully both for preventing and for halting the progression of MetS.
Postgraduate Program in Pharmacology, Department of Clinical and Toxicology Analysis, Center of Healthy Sciences, Federal University of Santa Maria (UFSM), 97105-900, Santa Maria, RS, Brazil. ksbona@yahoo.com.br
Karine S
KS
De Bona
Gabriela
G
Bonfanti
Lariane O
LO
Cargnelutti
Paula E R
PE
Bitencourt
Priscila S
PS
da Silva
Ronise
R
Ceolin
Victor C
VC
Pimentel
Maria Beatriz
MB
Moretto
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D004789:Enzyme Activation; D057075:Enzyme Assays; D005260:Female; D006801:Humans; D007249:Inflammation / Q000201:enzymology; D007328:Insulin / Q000378:metabolism; D015227:Lipid Peroxidation; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D024821:Metabolic Syndrome / Q000175:diagnosis / Q000201:enzymology*; D008875:Middle Aged; D012307:Risk Factors; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D048828:beta-N-Acetyl-Galactosaminidase / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D015415:Biomarkers; D007328:Insulin; D017392:Thiobarbituric Acid Reactive Substances; D005723:gamma-Glutamyltransferase; D048828:beta-N-Acetyl-Galactosaminidase; D018819:Dipeptidyl Peptidase 4