Evaluation of SNPs in miR-196-a2, miR-27a and miR-146a as risk factors of colorectal cancer.
OBJECTIVE
To investigate whether selected single nucleotide polymorphisms (SNPs) in miR-196a2, miR-27a and miR-146a genes are associated with sporadic colorectal cancer (CRC).


METHODS
In order to investigate the effect of these SNPs in CRC, we performed a case-control study of 197 cases of sporadic CRC and 212 cancer-free controls originating from the Central-European Caucasian population using TaqMan Real-Time polymerase chain reaction and allelic discrimination analysis.


RESULTS
The genotype and allele frequencies of SNPs were compared between the cases and the controls. None of the performed analysis showed any statistically significant results.


CONCLUSIONS
Our data suggest a lack of association between rs11614913, rs895819 and rs2910164 and colorectal cancer risk in the Central-European Caucasian population, a population with an extremely high incidence of sporadic colorectal cancer.
Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech.
Renata
R
Hezova
Alena
A
Kovarikova
Julie
J
Bienertova-Vasku
Milana
M
Sachlova
Martina
M
Redova
Anna
A
Vasku
Marek
M
Svoboda
Lenka
L
Radova
Igor
I
Kiss
Rostislav
R
Vyzula
Ondrej
O
Slaby
D000230:Adenocarcinoma / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D000368:Aged; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015179:Colorectal Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D015994:Incidence; D016015:Logistic Models; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D018570:Risk Assessment; D012307:Risk Factors; D044465:White People / Q000235:genetics
C542542:MIRN146 microRNA, human; C542543:MIRN196 microRNA, human; C531388:MIRN27 microRNA, human; D035683:MicroRNAs