Effects of inhibitors on the synergistic interaction between calpain and caspase-3 during post-mortem aging of chicken meat.
Calpain has been considered to be the most important protease involved in tenderization during the conversion of muscle into meat. However, recent evidence suggests the possible involvement of the key apoptosis protease, caspase, on post-mortem tenderization. This study used inhibitors of calpain and caspase-3 to treat chicken muscle immediately after slaughter and followed the changes in caspase-3 and calpain activities together with their expression during 5 days of aging. Addition of calpain inhibitors to the system resulted in significantly higher caspase-3 activities (p < 0.01) during storage. Western blot analysis of pro-caspase-3 and α-spectrin cleavage of the 120 kDa peptide (SBDP 120) showed that the addition of calpain inhibitors resulted in the formation of higher amounts of the active form of caspase-3 compared with the control (p < 0.01). Inclusion of inhibitors of caspase-3 led to lower calpain activities (p < 0.01) and dramatically reduced the expression of calpain-1 and calpain-2 (p < 0.01). Concomitantly, this inhibition resulted in greater calpastatin expression compared with the control (p < 0.01). The findings of this investigation show that calpain prevented the activation of caspase-3, whereas caspase-3 appeared to enhance the calpain activity during post-mortem aging through inhibition of calpastatin. It is therefore suggested that there is a relationship between caspase-3 and calpain which contributes to the tenderizing process during the conversion of muscle tissue into meat.
Key Laboratory of Meat Processing, Quality Control, Ministry of Education, Nanjing Agricultural University , Nanjing 210095, China.
Lin
L
Chen
Xian Chao
XC
Feng
Wan Gang
WG
Zhang
Xing Lian
XL
Xu
Guang Hong
GH
Zhou
D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002154:Calpain / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism*; D061945:Caspase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D002645:Chickens; D004151:Dipeptides / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology; D011200:Poultry; D011480:Protease Inhibitors / Q000737:chemistry / Q000494:pharmacology*
D002135:Calcium-Binding Proteins; D061945:Caspase Inhibitors; D004151:Dipeptides; D009842:Oligopeptides; D011480:Protease Inhibitors; C108637:aspartyl-glutamyl-valyl-aspartal; C071482:calpeptin; C033668:calpastatin; D002154:Calpain; D053148:Caspase 3; C058076:calpain inhibitor III