[Immunosuppression of human amniotic mesenchymal cells on allogeneic peripheral blood lymphocytes].
OBJECTIVE
To explore the immunoregulatory effects of human amniotic mesenchymal cells (hAMCs) on allogeneic peripheral blood lymphocytes.


METHODS
The hAMCs were isolated from abandoned human amnion. Peripheral blood mononuclear lymphocytes (PBMLs) were separated from healthy donors by density gradient centrifugation. Then, PBMLs were treated with phytohemagglutinin (PHA) and different concentrations of hAMCs. Proliferation effect of PBMLs was tested using MTS assay, and production of IFN-gamma and TNF-alpha by PBMLs was detected by ELISA.


RESULTS
hAMCs could remarkably inhibit the lymphocytes proliferation. When the ratios of hAMCs to PBMLs were 0.05: 1, 0.10 :1, 0.20: 1, the inhibitory rates of PBMLs proliferation were 16.91%, 20.83% and 28.19%, respectively. HAMCs also decreased the production of IFN-gamma and TNF-alpha by PBMLs in a dose-dependent manner (P<0.01).


CONCLUSIONS
HAMCs could inhibit the proliferation of allogeneic lymphocytes and reduce secretion of IFN-gamma and TNF-alpha, which might be one of the mechanism for prevention and remission of transplant rejection.
Institute of Clinical Medical Science, China-Japan Friendship Hospital, Beijing 100029, China. shujun2000cn@yahoo.com.cn
Jun
J
Shu
Ke-Hua
KH
Zhang
Hong
H
Li
Lin
L
Pan
Xiao-Jie
XJ
Huang
Ping
P
Wang
Wei-Gang
WG
Wang
Zhe
Z
Cai
D000650:Amnion / Q000166:cytology*; D049109:Cell Proliferation; D006801:Humans; D007108:Immune Tolerance; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008648:Mesoderm / Q000166:cytology*; D010835:Phytohemagglutinins / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D010835:Phytohemagglutinins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma