Mycoplasmateceae species are not found in Fallopian tubes of women with tubo-peritoneal infertility.
BACKGROUND
The role of mycoplasmas on the development and sequelae of pelvic inflammatory disease remains controversial. The objective of the present study is to correlate directly the presence of Mycoplasmateceae through polimerase chain reaction (PCR) determinations in cervix and Fallopian tubes of infertile patients with tubo-peritoneal factor diagnosed through laparoscopy.


METHODS
Thirty patients with tubo-peritoneal infertility and 30 normal fertile patients were included in the study; cervical samples and tubal flushings were obtained during laparoscopy. PCR determinations for the detection of genetic material of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealiticum, Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis in cervix and tubal flushings were performed.


RESULTS
No Mycoplasmataceae species as "only" microorganisms were found in tubal flushings of tubo-peritoneal infertility patients, whereas three (10%) fertile patients with normal tubes were positive for mycoplasma presence. This difference was not significant (p = 0.237). Among the 30 patients suffering from tubal infertility diagnosed through laparoscopy, Mycoplasmatecae species were not detected in the Fallopian tubes by PCR determinations, while in normal tubes from fertile patients these and other microorganisms could be found without distorting tubal anatomy.


CONCLUSIONS
Mycoplasmateceae species were not detected in Fallopian tubes of women with tubo-peritoneal infertility.
Departament of Obstetrics and Gynecology, Medical School, Universidad de Santiago de Chile, Santiago, Chile.
Alberto
A
Costoya
Francisco
F
Morales
Paula
P
Borda
Renato
R
Vargas
Juan
J
Fuhrer
Nicole
N
Salgado
Hugo
H
Cárdenas
Luis
L
Velasquez
D000328:Adult; D005184:Fallopian Tube Diseases / Q000382:microbiology*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000382:microbiology*; D060885:Multiplex Polymerase Chain Reaction; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000382:microbiology*; D045704:Mycoplasma genitalium / Q000302:isolation & purification; D019535:Mycoplasma hominis / Q000302:isolation & purification; D009178:Mycoplasmataceae / Q000145:classification / Q000302:isolation & purification*; D011446:Prospective Studies; D014509:Ureaplasma / Q000302:isolation & purification; D055815:Young Adult