Identification of imprinting regulators at the Meg3 differentially methylated region.
Genomic imprinting at the Delta-like 1 (Dlk1)-Maternally expressed gene 3 (Meg3) locus is regulated by the Meg3 differentially methylated region (DMR), but the mechanism by which this DMR acts is unknown. The goal of this study was to analyze the Meg3 DMR during imprinting establishment and maintenance for the presence of histone modifications and trans-acting DNA binding proteins using chromatin immunoprecipitation. In embryonic stem (ES) cells, where Meg3 is biallelically expressed, the DMR showed variable DNA methylation, with biallelic methylation at one region but paternal allele-specific methylation at another. All histone modifications detected at the Meg3 DMR of ES cells were biallelic. In embryonic day 12.5 (e12.5) embryos, where Meg3 is maternally expressed, the paternal Meg3 DMR was methylated, and activating histone modifications were specific to the maternal DMR. DNA-binding proteins that represent potential regulatory factors were identified in both ES cells and embryos.
Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA.
Erin N
EN
McMurray
Jennifer V
JV
Schmidt
D000483:Alleles; D000818:Animals; D002135:Calcium-Binding Proteins; D047369:Chromatin Immunoprecipitation; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D047108:Embryonic Development; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D044127:Epigenesis, Genetic; D056426:Genetic Loci; D018392:Genomic Imprinting; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D012737:Sex Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D002135:Calcium-Binding Proteins; C512925:Dlk1 protein, mouse; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; C422882:MEG3 non-coding RNA, mouse; D062085:RNA, Long Noncoding; D015534:Trans-Activators