Identification and functional characterization of protein kinase A phosphorylation sites in the major lipolytic protein, adipose triglyceride lipase.
Catecholamine-stimulated lipolysis occurs by activating adenylate cyclase and raising cAMP levels, thereby increasing protein kinase A (PKA) activity. This results in phosphorylation and modulated activity of several key lipolytic proteins. Adipose triglyceride lipase (ATGL) is the primary lipase for the initial step in triacylglycerol hydrolysis, and ATGL activity is increased during stimulated lipolysis. Here, we demonstrate that murine ATGL is phosphorylated by PKA at several serine residues in vitro and identify Ser(406) as a functionally important site. ATGL null adipocytes expressing ATGL S406A (nonphosphorylatable) had reduced stimulated lipolysis. Studies in mice demonstrated increased ATGL Ser(406) phosphorylation during fasting and moderate intensity exercise, conditions associated with elevated lipolytic rates. ATGL Ser(404) (corresponding to murine Ser(406)) phosphorylation was increased by β-adrenergic stimulation but not 5'AMP-activated protein kinase activation in human subcutaneous adipose tissue explants, which correlated with lipolysis rates. Our studies suggest that β-adrenergic activation can result in PKA-mediated phosphorylation of ATGL Ser(406), to moderately increase ATGL-mediated lipolysis.
Department of Physiology, Monash University, Clayton, 3800 Victoria, Australia.
Joanne
J
Pagnon
Maria
M
Matzaris
Romana
R
Stark
Ruth C R
RC
Meex
S Lance
SL
Macaulay
Wendy
W
Brown
Paul E
PE
O'Brien
Tony
T
Tiganis
Matthew J
MJ
Watt
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008066:Lipolysis / Q000235:genetics / Q000502:physiology; D051379:Mice; D010766:Phosphorylation; D016133:Polymerase Chain Reaction
D017868:Cyclic AMP-Dependent Protein Kinases; D008049:Lipase