[Effect of TLR4 on the migration of asthmatic airway smooth muscle cells induced by airway epithelial cells].
OBJECTIVE
To explore the effect of Toll-like receptor 4 (TLR4) activation on the migration of asthmatic airway smooth muscle cell (ASMCs) induced by airway epithelial cells.


METHODS
Primary ASMCs were cultured by the method of cell digestion. Cell culture supernatant of RTE cells were collected by TNF-alpha stimulation of epithelial cells. Detected the IL-8 and RANTES levels in the supernatant. The transmembrane migration of asthmatic ASMCs were detected by Modified Boyden chemotaxis chamber. The effect of TLR4 on the migration of asthmatic ASMCs induced by epithelial cells with TLR4 antibody drugs as a tool.


RESULTS
The levels of IL-8 and RANTES in the supernatant of TNF-alpha groups were significantly increased, and that in the 20 ng/ml group was significantly higher than other groups (P < 0.01). The transmembrane migration of asthmatic ASMCs groups was increased than that of control group. The transmembrane migration of asthmatic ASMCs from asthma group and TNF-alpha + TLR4 antibody group was significantly decreased compared with that in TNF-alpha group (P < 0.01). The migration of asthma ASMCs from TNF-alpha + TLR4 antibody group was increased than that of asthma group (P < 0.05).


CONCLUSIONS
TLR4 in the surface of asthmatic ASMCs may be activated by cytokines secreted by the airway epithelial cells and enhance the transmembrane migration of asthmatic ASMCs induced by airway epithelial cells so that it plays a role in airway remodeling of asthma.
Department of Respiratory Medicine, Hospital Affiliated Guilin Medical College, Guilin 541001, China.
Hai-Ying
HY
Su
Bi-Wen
BW
Mo
Jiang-Hong
JH
Wei
Jian-Wei
JW
Huang
Chang-Ming
CM
Wang
Jin-Rong
JR
Zeng
Qing
Q
Xu
Yun
Y
Lin
D000818:Animals; D001249:Asthma / Q000378:metabolism*; D002465:Cell Movement; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D018946:Chemokine CCL5; D016209:Interleukin-8; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha