Activity of MMP-9 after repair of abdominal wall defects with acellular and crosslinked bovine pericardium in rabbit.
This study was undertaken for the identification of matrix metalloproteinases (MMPs) in extracts obtained from native, acellular and crosslinked bovine pericardium (in vitro), as well as in the plasma after implantation of these biomaterials in rabbits (in vivo). Native pericardium (NP) expressed a 72 kDa (MMP-2) band; whereas, in acellular pericardium (AP) two bands (10 kDa and 92 kDa) of MMPs were observed of which, 92 kDa band was very faint. AP crosslinked with glutaraldehyde did not show any gelatinase activity and thus reflects the creation of new additional chemical bonds between the collagen molecules which has been effectively removed. Gelatin zymography showed only one major band of 92 kDa in all the implanted and untreated rabbit plasma, but the relative amount of 92 kDa was 1-2 times higher in acellular bovine pericardium implanted rabbits as compared to crosslinked and native groups. In NP group, the 92 kDa band was the dullest among the three groups. This indicated that the level of MMP-9 corresponds to the degree of collagen degradation.
Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, IndiaDivision of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, IndiaDepartment of Bioscience and Biotechnology, Banasthali Vidyapeeth, Rajasthan, IndiaDepartment of Biomedical technology, Jiwaji University, Gwalior, MP, India.
Himani
H
Singh
Naveen
N
Kumar
A K
AK
Sharma
Meena
M
Kataria
Ashok
A
Munjal
Amit
A
Kumar
Rukmani
R
Dewangan
Vineet
V
Kumar
J
J
Devarathnam
Sachin
S
Kumar
D058107:Abdominal Wound Closure Techniques; D000818:Animals; D002417:Cattle; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis* / Q000097:blood; D010496:Pericardium / Q000201:enzymology*; D011817:Rabbits
D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9