[Study on seeds dormancy release and physiological change of Thesium chinense].
OBJECTIVE
To study the seeds dormancy release and physiological change of Thesium chinense.


METHODS
To release dormancy, the seeds of T. chinense were treated with chemical reagent and stratification under 3-5 degrees C.


RESULTS
When washed with flowing water for 24 h, then soaked in 500 mg x L(-1) GA3 for 24 h, finally, treated with stratification method under 3-5 degrees C for 150 day, the split rate of T. chinense seeds reached 22%, crude fat decreased 50%, total sugar and dissolvability sugar increased 3-4 times, ABA decreased more than 90%, GA3 increased more than 5 times.


CONCLUSIONS
The seeds dormancy of T. chinense can be released with the method of washing with flowing water for 24 h, then soaking in 500 mg x L(-1) GA3 for 24 h, finally, treated with stratification method under 3-5 degrees C for 150-180 day.
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Fulai
F
Luo
Qiaosheng
Q
Guo
D000040:Abscisic Acid / Q000494:pharmacology; D002241:Carbohydrates / Q000032:analysis; D003080:Cold Temperature; D005223:Fats / Q000032:analysis; D018525:Germination / Q000502:physiology*; D005875:Gibberellins / Q000494:pharmacology; D019684:Magnoliopsida / Q000187:drug effects / Q000502:physiology*; D057445:Plant Dormancy / Q000187:drug effects / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010946:Plants, Medicinal / Q000187:drug effects / Q000502:physiology; D012639:Seeds / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D014867:Water / Q000494:pharmacology
D002241:Carbohydrates; D005223:Fats; D005875:Gibberellins; D010937:Plant Growth Regulators; D014867:Water; D000040:Abscisic Acid; C007842:gibberellic acid