Nanoporous gold electrode as a platform for the construction of an electrochemical DNA hybridization biosensor.
The application of a nanoporous gold electrode (NPGE) in the fabrication of an electrochemical sensing system for the detection of single base mismatches (SBMs) using ferrocene-modified DNA probe has been investigated in the present manuscript. Ferrocene carboxylic acid is covalently attached to the amino-modified probe using EDC/NHS chemistry. By covalent attachment of the redox reporter molecules on the top of DNA, the direct oxidation of the ferrocene on the electrode surface is avoided. On the other hand, the electrochemical signals are amplified by anodizing the electrode surface and converting it to nanoporous form. By improving the sensitivity of the biosensor, the different SBMs including the thermodynamically stable G-A and G-T mismatches, can be easily distinguished. In this research, NPGE was prepared by anodization and chemical reduction of Au surface and used for signal amplification. Nanoporous electrode enhances the sensitivity of DNA biosensor and makes it capable to detect complementary target DNA in sub-nanomole scales.
Department of Chemistry, Faculty of Science, University of Isfahan, Isfahan 81746-73441, Iran.
Laleh Enayati
LE
Ahangar
Masoud A
MA
Mehrgardi
D020137:Base Pair Mismatch; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D000075163:Metallocenes; D049329:Nanostructures / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016062:Porosity; D012680:Sensitivity and Specificity; D013816:Thermodynamics
D015342:DNA Probes; D005296:Ferrous Compounds; D000075163:Metallocenes; C032949:ferrocenecarboxylic acid; D006046:Gold; D004247:DNA