[Expression of fibroblast activation protein in HBV related hepatocellular carcinoma].
OBJECTIVE
To analyze the gene expression level of fibroblast activation protein in HBV related hepatocellular carcinoma patients and discuss its clinical significance.


METHODS
FAP gene expression in 33 hepatocellular carcinoma patients cancer tissues, peficancerous tissues, distant relative normal liver tissues and 13 normal liver tissues were examined by reverse transcription PCR; and real-time fluorescent quantitative PCR (qRT-PCR) was used to quantify their expression.


RESULTS
FAP were expressed in all the tissues,the relative expression values in cancer tissues, peficancerous tissues and distant relative normal liver tissues were 5.14 +/- 6.69, 1.58 +/- 0.96, 1.63 +/- 0.94, respectively, the differences were statistically significant (F = 4.401, P < 0.05); and in TNM stage I, II, IIII, they were 2.89 +/- 3.35, 4.15 +/- 4.69, 10.09 +/- 9.51 respectively; in well-differentiated, differentiated and poorly differentiated hepatocellular carcinoma were 1.62 +/- 1.74, 3.84 +/- 3.79, 1.26 +/- 13.34 respectively. The differences were all statistically significant (P < 0.05).


CONCLUSIONS
FAP may play an important role in the occurrence and development of HBV related hepatocellular carcinoma.
Department of Infectious Disease, Third Affiliated Hospital of Sun-Yat-Sen University, Guangzhou 510630, China.
Ye-Qiong
YQ
Zhang
Jian-Xi
JX
Lu
Hai-Xia
HX
Sun
Xin
X
Shu
Hong
H
Cao
Xing-Fei
XF
Pan
Qi-Huan
QH
Xu
Gang
G
Li
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000378:metabolism; D010450:Endopeptidases; D005260:Female; D018093:Gelatinases / Q000235:genetics / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D006509:Hepatitis B / Q000150:complications*; D006801:Humans; D008113:Liver Neoplasms / Q000209:etiology* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D012697:Serine Endopeptidases / Q000235:genetics / Q000502:physiology*; D000099167:Fibroblast Activation Protein Alpha
D010450:Endopeptidases; D018093:Gelatinases; D008565:Membrane Proteins; D012333:RNA, Messenger; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha