[Effect of water deficit on gene expression of enzymes related with hydrogen peroxide detoxification system in Scutellaria baicalensis].
OBJECTIVE
To analysis the effects of water deficit on the transcript level of SOD, APX, DHAR and MDHAR genes in Scutellaria baicalensis.


METHODS
Three-month-old S. baicalensis was in glasshouse under water deficit stress, and the transcript level of SOD, APX, DHAR and MDHAR genes were analysis utilized semi-quantitative RT-PCR.


RESULTS
Compared with the control group, a significant decline of the transcriptional level of APX gene was observed at 70 days after water deficit. The transcript level of DHAR gene was reduced at 30 and 50 days after water deficit. And MDHARI gene was significant declined at 50 days.


CONCLUSIONS
AsA which is an important antioxidant plays a major role in hydrogen peroxide clear system under water deficit, and maybe have an antagonistic effect to the accumulation of baicalein.
Wuhan Polythechnic University, Wuhan 430023, China.
Chong
C
Wu
Shuangshuang
S
Qin
Yuan
Y
Yuan
Ping
P
Chen
Shuifang
S
Lin
D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D031364:Scutellaria baicalensis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014867:Water / Q000378:metabolism / Q000494:pharmacology*
D010940:Plant Proteins; D014867:Water; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D060387:Ascorbate Peroxidases; D013482:Superoxide Dismutase; D009247:NADH, NADPH Oxidoreductases; C023927:monodehydroascorbate reductase (NADH); C020666:glutathione dehydrogenase (ascorbate); D001205:Ascorbic Acid