Study on impurities in naringin extracted from Citrus grandis 'tomentosa'.
OBJECTIVE
To investigate the impurities in naringin extracted from Citrus grandis 'Tomentosa'.


METHODS
High performance liquid chromatographies coupled with photodiode array and electrospray ionization mass spectrometry detectors (HPLC-PDA/ESI-MS/MS) were applied to investigate the impurities, and their structures were elucidated by spectral data analyses. Quantification was carried out by main component self-compare with correction factor according to ICH guidelines.


RESULTS
Rhoifolin and neoeriocitrin were identified as major impurities. The correction factors of rhoifolin and neoeriocitrin were 1.82 and 1.02, respectively tested by HPLC method. The content of rhoifolin ranged from 0.742% to 0.926%, and the content of neoeriocitrin ranged from 0.335% to 0.464%. The gross impurities were less than 1.5%.


CONCLUSIONS
The categories and quantities of impurities in naringin product are relatively stable. The research provides a way of specification and verification for the analysis of impurities and objective evidence for security assessment of naringin product.
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. liumenghua_one@126.com
Meng-Hua
MH
Liu
Hai-Bin
HB
Liu
Wei-Wei
WW
Su
Xiao-Rou
XR
Liu
Jia-He
JH
Li
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis* / Q000737:chemistry; D044950:Flavanones / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D000960:Hypolipidemic Agents / Q000737:chemistry; D015394:Molecular Structure; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D005947:Glucose / Q000031:analogs & derivatives; D008358:Mannose / Q000031:analogs & derivatives; D047310:Apigenin
D004187:Disaccharides; D044950:Flavanones; D005419:Flavonoids; D006027:Glycosides; D000960:Hypolipidemic Agents; C553460:neoeriocitrin; C089378:rhoifolin; D005947:Glucose; D008358:Mannose; D047310:Apigenin; C005274:naringin