Evaluation of modified vaccinia virus Ankara expressing VP2 protein of infectious bursal disease virus as an immunogen in chickens.
A recombinant modified vaccinia Ankara (MVA) virus expressing mature viral protein 2 (VP2) of the infectious bursal disease virus (IBDV) was constructed to develop MVA-based vaccines for poultry. We demonstrated that this recombinant virus was able to induce a specific immune response by observing the production of anti-IBDV-seroneutralizing antibodies in specific pathogen-free chickens. Besides, as the epitopes of VP2 responsible to induce IBDV-neutralizing antibodies are discontinuous, our results suggest that VP2 protein expressed from MVA-VP2 maintained the correct conformational structure. To our knowledge, this is the first report on the usefulness of MVA-based vectors for developing recombinant vaccines for poultry.
Consejo Nacional de Investigaciones Científicas y Técnicas, Rivadavia 1917 (C1033AAJ) Ciudad Autónoma de Buenos Aires, Argentina. fzanetti@cnia.inta.gov.ar
Flavia Adriana
FA
Zanetti
María Paula
MP
Del Médico Zajac
Oscar Alberto
OA
Taboga
Gabriela
G
Calamante
D000818:Animals; D000914:Antibodies, Viral; D018175:Birnaviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D005347:Fibroblasts / Q000378:metabolism; D007243:Infectious bursal disease virus / Q000276:immunology*; D011201:Poultry Diseases / Q000517:prevention & control* / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins; D014765:Viral Vaccines