Effectiveness of a dynein team in a tug of war helped by reduced load sensitivity of detachment: evidence from the study of bidirectional endosome transport in D. discoideum.
Bidirectional cargo transport by molecular motors in cells is a complex phenomenon in which the cargo (usually a vesicle) alternately moves in retrograde and anterograde directions. In this case, teams of oppositely pulling motors (e.g., kinesin and dynein) bind to the cargo, simultaneously, and 'coordinate' their activity such that the motion consists of spells of positively and negatively directed segments, separated by pauses of varying duration. A set of recent experiments have analyzed the bidirectional motion of endosomes in the amoeba D. discoideum in detail. It was found that in between directional switches, a team of five to six dyneins stall a cargo against a stronger kinesin in a tug of war, which lasts for almost a second. As the mean detachment time of a kinesin under its stall load was also observed to be ∼1 s, we infer that the collective detachment time of the dynein assembly must also be similar. Here, we analyze this inference from a modeling perspective, using experimentally measured single-molecule parameters as inputs. We find that the commonly assumed exponential load-dependent detachment rate is inconsistent with observations, as it predicts that a five-dynein assembly will detach under its combined stall load in less than a hundredth of a second. A modified model where the load-dependent unbinding rate is assumed to saturate at stall-force level for super-stall loads gives results which are in agreement with experimental data. Our analysis suggests that the load-dependent detachment of a dynein in a team is qualitatively different at sub-stall and super-stall loads, a conclusion which is likely to have implications in other situations involving collective effects of many motors.
Department of Physics, Indian Institute of Technology Madras, Chennai 600036, India. deepak@physics.iitm.ac.in
Deepak
D
Bhat
Manoj
M
Gopalakrishnan
D001692:Biological Transport; D003198:Computer Simulation; D004023:Dictyostelium / Q000378:metabolism*; D004398:Dyneins / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism*; D016547:Kinesins / Q000378:metabolism; D008954:Models, Biological; D015800:Protozoan Proteins / Q000378:metabolism*; D013269:Stochastic Processes
D015800:Protozoan Proteins; D004398:Dyneins; D016547:Kinesins