New triterpenoid saponins from cacti and anti-type I allergy activity of saponins from cactus.
The research in our laboratory focuses on the isolation of saponins from cactus. In this study, we report five new triterpenoid saponins, dumortierinoside A methyl ester (1), pachanoside I1 (2), pachanoside D1 (3), gummososide A (4), and gummososide A methyl ester (5). Compounds 1-3 isolated from Isolatocereus dumortieri Backbg., and compounds 4 and 5 were isolated from Stenocereus alamosensis A. C. Gibson & K. E. Horak. Compound 2 possessed a new pachanane-type triterpene skeleton, pachanol I, in its aglycon. The aglycon of 3 was pachanol D, while those of 4 and 5 were both gummosogenin, which we have previously reported, but this is the first report of pachanol D and gummosogenin in their aglycon forms. Additionally, we evaluated the anti-type I allergy activity of the saponins with RBL-2H3 (Rat basophilic leukemia) cells by measuring the β-hexosaminidase release inhibitory activity. As a result of these studies, gummososide A methyl ester (5) was found to show activity (IC(50)=99.5 μM) and thurberoside A exhibited mild activity (IC(50)=166.9 μM).
Department of Pharmacognosy and Phytochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose-shi, Tokyo 204-8588, Japan.
Kazutaka
K
Kakuta
Masaki
M
Baba
Satoru
S
Ito
Kaoru
K
Kinoshita
Kiyotaka
K
Koyama
Kunio
K
Takahashi
D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D029421:Cactaceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D008958:Models, Molecular; D015394:Molecular Structure; D051381:Rats; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D018926:Anti-Allergic Agents; D012503:Saponins; D014315:Triterpenes; D001619:beta-N-Acetylhexosaminidases