Expression and characterization of α-methylacyl CoA racemase from Anisakis simplex larvae.
Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as α-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-40℃) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae.
Department of Internal Medicine, Kosin University College of Medicine, Busan 602-703, Korea.
Bong Jin
BJ
Kim
Sun Mi
SM
Kim
Min Kyung
MK
Cho
Hak Sun
HS
Yu
Yong Seok
YS
Lee
Hee Jae
HJ
Cha
Meesun
M
Ock
D000595:Amino Acid Sequence; D000818:Animals; D017163:Anisakis / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015723:Gene Library; D006801:Humans; D007150:Immunohistochemistry; D007814:Larva / Q000201:enzymology / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D010802:Phylogeny; D011817:Rabbits; D019998:Racemases and Epimerases / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D019998:Racemases and Epimerases; C108352:alpha-methylacyl-CoA racemase