Quaternized chitosan-coated nanofibrous materials containing gossypol: preparation by electrospinning, characterization and antiproliferative activity towards HeLa cells.
Nanofibrous polylactide-based materials loaded with a natural polyphenolic compound gossypol (GOS) with antitumor properties were prepared by electrospinning. The nanofibrous materials were coated with a thin film of crosslinked quaternized chitosan (QCh). GOS incorporated in the nanofibrous mats was in the amorphous state. GOS release was diffusion-controlled and its in vitro release profiles depended on the mat composition. The nanofibrous materials exhibited high cytotoxicity towards HeLa tumor cells. Interestingly, it was particularly pronounced in the case of fibrous materials, which contain both QCh and GOS. The observed strong antiproliferative effect of the nanofibrous mats was mainly due to induction of cell apoptosis.
Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., bl. 103A, BG-1113 Sofia, Bulgaria.
Milena
M
Ignatova
Nevena
N
Manolova
Reneta
R
Toshkova
Iliya
I
Rashkov
Elena
E
Gardeva
Liliya
L
Yossifova
Marin
M
Alexandrov
D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000633:toxicity; D004339:Drug Compounding; D016503:Drug Delivery Systems; D006072:Gossypol / Q000737:chemistry* / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry / Q000633:toxicity; D011092:Polyethylene Glycols / Q000737:chemistry / Q000633:toxicity
D011091:Polyesters; D011092:Polyethylene Glycols; C033616:poly(lactide); D048271:Chitosan; D006072:Gossypol