Rapid ultrastructural detection of success or failure after bone marrow transplantation in the Chediak-Higashi syndrome.
The present study has used electron microscopic techniques to rapidly detect the success or failure of bone marrow transplantation in three patients with the Chediak-Higashi Syndrome (CHS). The most rapid procedure was the whole mount technique to determine the presence or absence of dense bodies, which are inherently electron-opaque, serotonin-containing storage organelles in platelets. Dense bodies were present in normal numbers in platelets from two patients with successful transplantation and absent in thrombocytes from another patient in whom the transplant had failed.
Department of Laboratory Medicine, Pathology and Pediatrics, University of Minnesota School of Medicine, Minneapolis, MN, USA. White003@umn.edu
James G
JG
White
Richard A
RA
Hess
William A
WA
Gahl
Wendy
W
Introne
D001792:Blood Platelets / Q000648:ultrastructure*; D016026:Bone Marrow Transplantation; D002609:Chediak-Higashi Syndrome / Q000175:diagnosis / Q000473:pathology* / Q000628:therapy; D002675:Child, Preschool; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D006801:Humans; D007223:Infant; D007962:Leukocytes / Q000648:ultrastructure; D011379:Prognosis; D016896:Treatment Outcome