In vitro trans-translation assays.
Trans-translation is a bacterial quality control system in protein synthesis facilitated by transfer-messenger RNA (tmRNA). Here, we describe the in vitro system using purified factors to evaluate the two steps of trans-translation: peptidyl-transfer from peptidyl-tRNA to alanyl-tmRNA and decoding of the resume codon on tmRNA.
Department of Biochemistry and Molecular Biology, Hirosaki University, Hirosaki, Japan.
Daisuke
D
Kurita
Akira
A
Muto
Hyouta
H
Himeno
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D003062:Codon / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology; D010445:Peptide Elongation Factors / Q000378:metabolism; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism
D003062:Codon; D010445:Peptide Elongation Factors; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; C019037:tRNA, peptidyl-; C096222:tmRNA; D000604:Amino Acyl-tRNA Synthetases