Yeast fermentation of carboxylic acids obtained from pyrolytic aqueous phases for lipid production.
The presence of very reactive C1-C4 molecules adversely affects the quality bio-oils produced from the pyrolysis of lignocellulosic materials. In this paper a scheme to produce lipids with Cryptococcus curvatus from the carboxylic acids in the pyrolytic aqueous phase collected in fractional condensers is proposed. The capacities of three oleaginous yeasts C. curvatus, Rhodotorula glutinis, Lipomyces starkeyi to ferment acetate, formate, hydroxylacat-aldehyde, phenol and acetol were investigated. While acetate could be a good carbon source for lipid production, formate provides additional energy and contributes to yeast growth and lipid production as auxiliary energy resource. Acetol could slightly support yeast growth, but it inhibits lipid accumulation. Hydroxyacetaldehyde and phenols showed high yeast growth and lipid accumulation inhibition. A pyrolytic aqueous phase with 20 g/L acetate was fermented with C. curvatus, after neutralization and detoxification to produce 6.9 g/L dry biomass and 2.2 g/L lipid.
Biological Systems Engineering, Washington State University, WA 99164-6120, USA.
Jieni
J
Lian
Manuel
M
Garcia-Perez
Ralph
R
Coates
Hongwei
H
Wu
Shulin
S
Chen
D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000494:pharmacology; D000085:Acetates / Q000378:metabolism; D000096:Acetone / Q000031:analogs & derivatives / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002264:Carboxylic Acids / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation / Q000187:drug effects / Q000502:physiology*; D005561:Formates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis*; D010636:Phenols / Q000494:pharmacology; D013696:Temperature; D015003:Yeasts / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D000085:Acetates; D002264:Carboxylic Acids; D005227:Fatty Acids; D005561:Formates; D008055:Lipids; D010636:Phenols; C030544:formic acid; D000096:Acetone; C004433:acetol; D000079:Acetaldehyde; C010972:glycolaldehyde