Isolation and structure determination of new siderophore tsukubachelin B from Streptomyces sp. TM-74.
The new siderophore tsukubachelin B (1) was isolated from the iron-deficient culture medium of the newly isolated strain Streptomyces sp. TM-74. The chemical structure of tsukubachelin B (1) was established via interpretation of 2D nuclear magnetic resonance and electrospray ionization-mass spectroscopic data. The structure of tsukubachelin B (1) comprises 6 mol of amino acids, including 2 mol of serine and 1 mol each of ornithine, N-α-methyl-N-δ-hydroxy-N-δ-formylornithine, N-α-methyl-N-δ-hydroxyornithine, and cyclic N-hydroxyornithine.
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Suruga-ku, Shizuoka, Japan. askodan@ipc.shizuoka.ac.jp
Shinya
S
Kodani
Fumiya
F
Kobayakawa
Michitaka
M
Hidaki
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry* / Q000302:isolation & purification*; D017262:Siderophores / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism; D013302:Streptomyces / Q000378:metabolism*
D000806:Angiotensin-Converting Enzyme Inhibitors; D009842:Oligopeptides; D017262:Siderophores; C585506:tsukubachelin B