Characterization and in-vitro bioactivity evaluation of paclitaxel-loaded polyester nanoparticles.
Paclitaxel, an antimicrotubular agent used in the treatment of ovarian and breast cancer, was encapsulated in nanoparticles of poly(DL-lactide-co-glycolide) and poly(ε-caprolactone) polymers using the double emulsion-solvent evaporation technique. The morphology, size distribution, drug encapsulation efficiency, thermal degradation and in-vitro drug release profile were characterized. High-performance liquid chromatography was used to determine the drug encapsulation efficiency and in-vitro drug release profile. MCF-7 breast cancer cells were used to evaluate the cytotoxicity (MTT assay), the cellular uptake and the cell cycle. The particle size was in the range of 200-400 nm. Poly(lactide-co-glycolide) nanoparticles showed more effective cellular uptake compared with those of poly(ε-caprolactone). Unloaded nanoparticles were found to be cytocompatible on MCF-7 cells and paclitaxel formulations showed efficacy in killing MCF-7 cells. Paclitaxel-loaded nanoparticles induced the release of the drug-blocking cells in the G2/M phase. Paclitaxel-loaded nanoparticles may be considered a promising drug delivery system in the evaluation of an in-vivo model.
Department of Pharmacology, Complutense University of Madrid, Madrid, Spain.
Patricia
P
López-Gasco
Irene
I
Iglesias
Juana
J
Benedí
Rafael
R
Lozano
María Dolores
MD
Blanco
D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D004705:Endocytosis / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D017239:Paclitaxel / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011098:Polyglactin 910 / Q000737:chemistry*; D012995:Solubility; D013499:Surface Properties; D013818:Thermogravimetry
D004337:Drug Carriers; D011091:Polyesters; C016240:polycaprolactone; D011098:Polyglactin 910; D017239:Paclitaxel