Nanowire transistor-based ultrasensitive virus detection with reversible surface functionalization.
We have applied a reusable silicon nanowire field-effect transistor (SiNW-FET) as a biosensor to conduct ultrasensitive detection of H5N2 avian influenza virus (AIV) in very dilute solution. The reversible surface functionalization of SiNW-FET was made possible using a disulfide linker. In the surface functionalization, 3-mercaptopropyltrimethoxysilane (MPTMS) was first modified on the SiNW-FET (referred to as MPTMS/SiNW-FET), with subsequent dithiothreitol washing to reduce any possible disulfide bonding between the thiol groups of MPTMS. Subsequently, receptor molecules could be immobilized on the MPTMS/SiNW-FET by the formation of a disulfide bond. The success of the reversible surface functionalization was verified with fluorescence examination and electrical measurements. A surface topograph of the SiNW-FET biosensor modified with a monoclonal antibody against H5N2 virus (referred to as mAb(H5)/SiNW-FET) after detecting approximately 10(-17) M H5N2 AIVs was scanned by atomic force microscopy to demonstrate that the SiNW-FET is capable of detecting very few H5N2 AIV particles.
Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan, R.O.C.
Pei-Ling
PL
Chiang
Tzu-Chi
TC
Chou
Tzu-Heng
TH
Wu
Chang-Chi
CC
Li
Chun-Da
CD
Liao
Jeng-Yu
JY
Lin
Ming-Hsueh
MH
Tsai
Chia-Chang
CC
Tsai
Chih-Jung
CJ
Sun
Ching-Ho
CH
Wang
Jim-Min
JM
Fang
Yit-Tsong
YT
Chen
D000818:Animals; D055910:Antibodies, Immobilized / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D015374:Biosensing Techniques; D001717:Birds / Q000821:virology; D053125:Influenza A Virus, H5N2 Subtype / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000821:virology; D053770:Nanowires / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties; D014173:Transistors, Electronic
D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D012821:Silanes; D012825:Silicon