Enhancing effect of human thyroid stimulating hormone (hTSH) monoclonal antibody in the hTSH immunoradiometric assay (IRMA) system.
Different monoclonal antibodies, both commercial and indigenously produced, were evaluated in various combinations to optimize an immunoradiometric assay (IRMA) system for human thyroid stimulating hormone (hTSH). During these studies, it was observed that mixing one of the indigenously produced hTSH monoclonal antibody (2B11) in the hTSH IRMA system using Immunotech (Beckman Coulter, Czech Republic) kit reagents, led to an overall increase in the assay binding and sensitivity (from 0.025 mIU/L to 0.015mIU/L) of this IRMA system. This is not a general property of all monoclonal antibodies against hTSH. The mechanism for this enhancement can be attributed to the formation of a multicomponent complex.
Department of Atomic Energy, Radioimmunoassay Group, Radiopharmaceuticals Programme, Board of Radiation and Isotope Technology, Navi Mumbai, India. shubhangi.mirapurkar@rediffmail.com
Shubhangi
S
Mirapurkar
G
G
Samuel
S D
SD
Borkute
N
N
Sivaprasad
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D045744:Cell Line, Tumor; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D015592:Immunoradiometric Assay; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D013972:Thyrotropin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D013972:Thyrotropin