Microarray analysis of the gene expression profile of HMC-1 mast cells following Schizonepeta tenuifolia Briquet treatment.
It has long been believed that mast cells play a crucial role in the development of many physiological changes during immediate allergic responses. This study was conducted to evaluate the anti-inflammation mechanism of Schizonepeta tenuifolia (ST) extract and ST purified chemicals on the PMA plus A23187-induced stimulation of HMC-1 human mast cells. ST, rosmarinic acid, pulegone, and 2α,3α,24-thrihydrooxylen-12en-28oic acid treatment of HMC-1 cells led to significant suppression of pro-inflammatory cytokines (IL-6, IL-8, and TNF-α) in a dose dependent manner. In addition, the results of the microarray and real-time RT-PCR analyses revealed that ST regulates several pathways, including the cytokine-cytokine receptor interaction (CCRI), MAPK, and the Toll-like receptor (TLR) signaling pathways. ST may be useful for the treatment of inflammation disease via anti-inflammation activity that occurs through inhibition of the CCRI, MAPK, and TLR signaling pathways.
Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
Sung-Hwa
SH
Sohn
Sunim
S
Cho
Eun Seok
ES
Ji
Sung-Hoon
SH
Kim
Minkyu
M
Shin
Moochang
M
Hong
Hyunsu
H
Bae
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D061207:Calcium Ionophores / Q000494:pharmacology; D002460:Cell Line; D002934:Cinnamates / Q000494:pharmacology; D000081005:Cyclohexane Monoterpenes; D016207:Cytokines / Q000096:biosynthesis; D053630:Depsides / Q000494:pharmacology; D020869:Gene Expression Profiling; D006801:Humans; D019686:Lamiaceae; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D039821:Monoterpenes / Q000494:pharmacology; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D000097123:Rosmarinic Acid
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000001:Calcimycin; D061207:Calcium Ionophores; D002934:Cinnamates; D000081005:Cyclohexane Monoterpenes; D016207:Cytokines; D053630:Depsides; D039821:Monoterpenes; D009828:Oleanolic Acid; D010936:Plant Extracts; D000097123:Rosmarinic Acid; C575836:2,3,24-trihydroxyolean-12-en-28-oic acid; C039648:pulegone