Analysis of helicase-RNA interactions using nucleotide analog interference mapping.
Nucleotide analog interference mapping (NAIM) is a combinatorial approach that probes individual atoms and functional groups in an RNA molecule and identifies those that are important for a specific biochemical function. Here, we show how NAIM can be adapted to reveal functionally important atoms and groups on RNA substrates of helicases. We explain how NAIM can be used to investigate translocation and unwinding mechanisms of helicases and discuss the advantages and limitations of this powerful chemogenetic approach.
CNRS UPR4301, Centre de Biophysique Moléculaire, Orléans cedex 2, France.
Annie
A
Schwartz
Makhlouf
M
Rabhi
Emmanuel
E
Margeat
Marc
M
Boudvillain
D000818:Animals; D006801:Humans; D009710:Nucleotide Mapping / Q000379:methods*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism*; D020365:RNA Helicases / Q000378:metabolism*
D012313:RNA; D020365:RNA Helicases