One-pot synthesis of fluorescent polysaccharides: adenine grafted agarose and carrageenan.
New fluorescent polysaccharides were synthesized by grafting the nucleobase adenine on to the backbones of agarose and κ-carrageenan, which were characterized by FT-IR, (13)C NMR, TGA, XRD, UV, and fluorescence properties. The synthesis involved a rapid water based potassium persulfate (KPS) initiated method under microwave irradiation. The emission spectra of adenine grafted agarose and κ-carrageenan were recorded in aqueous (5×10(-5) M) solution, exhibiting λ(em,max) 347 nm by excitation at 261 nm, affording ca. 30% and 40% enhanced emission intensities, respectively compared to that of pure adenine solution in the same concentration. Similar emission intensity was recorded in the pure adenine solution at its molar equivalent concentrations present in the 5×10(-5) M solution of the agarose and carrageenan grafted products, that is, 3.28×10(-5) M and 4.5×10(-5) M respectively. These fluorescent adenine grafted products may have potential utility in various sensor applications.
Marine Biotechnology & Ecology Discipline, CSIR-Central Salt & Marine Chemicals Research Institute (Council of Scientific & Industrial Research), Bhavnagar 364 002, Gujarat, India.
Mihir D
MD
Oza
Kamalesh
K
Prasad
A K
AK
Siddhanta
D000225:Adenine / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D002236:Carbohydrate Conformation; D002351:Carrageenan / Q000737:chemistry* / Q000648:ultrastructure; D002942:Circular Dichroism; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D008872:Microwaves; D015394:Molecular Structure; D017680:Potassium Compounds / Q000737:chemistry; D012685:Sepharose / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000648:ultrastructure; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000737:chemistry; D013818:Thermogravimetry; D014783:Viscosity; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
D005456:Fluorescent Dyes; D017680:Potassium Compounds; D012997:Solvents; D013431:Sulfates; D014867:Water; C009007:potassium persulfate; D002351:Carrageenan; D012685:Sepharose; D000225:Adenine