[Anticoagulative and anticomplementary activity of endogenous inhibitor preparation from hepatopancreas of red king crab (Paralithosed camtschaticus) towards human blood].
Serpins (SERine Protease INhibitors)--is large and diverse group of proteins with similar structures, which can inhibit both serine and cysteine proteases by an irreversible suicide mechanism. A novel serpin from hepatopancreas of Red King Crab (Paralithosed camtschaticus) was obtained and was studied its effect on the process of human blood plasma clotting. The investigated serpin shows a noticeable anticoagulative activity, which increases dramatically in the combined action with heparine. Though the inhibitor has almost no effect on thrombin, it inhibits C1s (C1-esterase). We studied the action of the serpin from P. camtschaticus on C1s via its competitive inhibition by C1 inhibitor and the novel enzyme. The calculated inhibition constant of the serpin from P. camtschaticus towards C1s is 2.02 +/- 0.71 M. Unlike C1 inhibitor, the novel serpin from P. camtschaticus doesn't suppress fibrinolysis and at the same time prevents blood clotting. These features may be of interest for medical purposes.
M V
MV
Balashova
L V
LV
Liutova
Iu A
IuA
Rudenskaia
V A
VA
Isaev
S S
SS
Andina
L V
LV
Kozlov
G N
GN
Rudenskaia
D000818:Animals; D033381:Anomura / Q000737:chemistry*; D000925:Anticoagulants / Q000494:pharmacology*; D001667:Binding, Competitive; D001777:Blood Coagulation / Q000187:drug effects; D003172:Complement C1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D005341:Fibrinolysin / Q000037:antagonists & inhibitors; D006493:Heparin / Q000494:pharmacology; D043143:Hepatopancreas / Q000737:chemistry; D006801:Humans; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D015843:Serpins / Q000378:metabolism / Q000494:pharmacology*; D013917:Thrombin / Q000037:antagonists & inhibitors
D000925:Anticoagulants; D003172:Complement C1; D015842:Serine Proteinase Inhibitors; D015843:Serpins; D006493:Heparin; D013917:Thrombin; D005341:Fibrinolysin