[Effects of long-term external use of goupi gao on renal functions and lead accumulation in rats].
OBJECTIVE
To observe the effect of long-term external use of Goupi Gao on renal function and lead accumulation in rats.


METHODS
Rats were externally administered with Goupi Gao at different doses (7, 3.5 and 1.75 g x kg(-1)) for 90 d. At 45 days and 90 days after administration, the renal indicator, levels of blood urea nitrogen (BU) and creatinine (Cr) in serum, beta2-microglobulin (beta2-MG) and N-acetyl-beta-D-glucosaminidase (NAG) in urine were determined. Lead content in kidneys was detected by atomic absorption spectrometry.


RESULTS
A 90-day administration with Goupi Gao significantly enhanced the renal indicator, levels of NAG in urine and lead content in renal, when compared with the normal rats. However, the levels of BUN and beta2-MG as well as renal pathology in Goupi Gao treated rats were not obviously changed.


CONCLUSIONS
Consecutive administration of Goupi Gao for 90 days can increase the renal indicator and levels of NAG in urine, enhance the accumulation of lead in renal, but with no effect on excretory function of kidneys and organic changes.
Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese Herbal Medicines of Ministry of Education, Chengdu 610075, China.
Yong
Y
Zeng
Fanfan
F
Li
Chungang
C
Li
Huaibin
H
Chen
Guiqin
G
Zhao
Xianli
X
Meng
D000118:Acetylglucosaminidase / Q000652:urine; D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D007854:Lead / Q000032:analysis / Q000378:metabolism*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013054:Spectrophotometry, Atomic; D001613:beta 2-Microglobulin / Q000652:urine
D004365:Drugs, Chinese Herbal; D001613:beta 2-Microglobulin; D007854:Lead; D003404:Creatinine; D000118:Acetylglucosaminidase