T-cell immune monitoring by the ELISPOT assay for interferon gamma.
The enzyme-linked immunosorbent spot (ELISPOT) assay for interferon gamma has been available for more than twenty years and has been used for a number of applications, including the monitoring of T cell immunity in solid organ transplant recipients. Studies from single centers indicate that heightened T cell alloreactivity measured with this assay correlates with acute and chronic rejection and with poor long-term allograft function. The assay has been used not only to assess T cell reactivity after transplantation, but also as a tool for assessing immune risk prior to transplantation. Additional work is needed to validate the assay in larger multicenter clinical trials.
Division of Nephrology and Hypertension, Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, Ohio 44116, USA.
Joshua J
JJ
Augustine
Donald E
DE
Hricik
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006084:Graft Rejection / Q000209:etiology; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis* / Q000276:immunology; D015166:Monitoring, Immunologic; D016377:Organ Transplantation / Q000009:adverse effects; D018570:Risk Assessment; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous / Q000009:adverse effects
D007371:Interferon-gamma