Isolation of ZnO-binding 12-mer peptides and determination of their binding epitopes by NMR spectroscopy.
Inorganic-binding peptides are in the focus of research fields such as materials science, nanotechnology, and biotechnology. Applications concern surface functionalization by the specific coupling to inorganic target substrates, the binding of soluble molecules for sensing applications, or biomineralization approaches for the controlled formation of inorganic materials. The specific molecular recognition of inorganic surfaces by peptides is of major importance for such applications. Zinc oxide (ZnO) is an important semiconductor material which is applied in various devices. In this study the molecular fundamentals for a ZnO-binding epitope was determined. 12-mer peptides, which specifically bind to the zinc- or/and the oxygen-terminated sides of single-crystalline ZnO (0001) and (000-1) substrates, were selected from a random peptide library using the phage display technique. For two ZnO-binding peptides the mandatory amino acid residues, which are of crucial importance for the specific binding were determined with a label-free nuclear magnetic resonance (NMR) approach. NMR spectroscopy allows the identification of pH dependent interaction sites on the atomic level of 12-mer peptides and ZnO nanoparticles. Here, ionic and polar interaction forces were determined. For the oxygen-terminated side the consensus peptide-binding sequence (HSXXH) was predicted in silico and confirmed by the NMR approach.
Institute for Materials Science, University of Stuttgart, 70569 Stuttgart, Germany. dirk.rothenstein@imw.uni-stuttgart.de
Dirk
D
Rothenstein
Birgit
B
Claasen
Beatrice
B
Omiecienski
Patricia
P
Lammel
Joachim
J
Bill
D000595:Amino Acid Sequence; D001665:Binding Sites; D019906:Nuclear Magnetic Resonance, Biomolecular; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D016415:Sequence Alignment; D015034:Zinc Oxide / Q000378:metabolism*
D019151:Peptide Library; D010455:Peptides; D015034:Zinc Oxide