Biointerface: protein enhanced stem cells binding to implant surface.
The number of metallic implantable devices placed every year is estimated at 3.7 million. This number has been steadily increasing over last decades at a rate of around 8 %. In spite of the many successes of the devices the implantation of biomaterial into tissues almost universally leads to the development of an avascular sac, which consists of fibrous tissue around the device and walls off the implant from the body. This reaction can be detrimental to the function of implant, reduces its lifetime, and necessitates repeated surgery. Clearly, to reduce the number of revision surgeries and improve long-term implant function it is necessary to enhance device integration by modulating cell adhesion and function. In this paper we have demonstrated that it is possible to enhance stem cell attachment using engineered biointerfaces. To create this functional interface, samples were coated with polymer (as a precursor) and then ion implanted to create a reactive interface that aids the binding of biomolecules--fibronectin. Both AFM and XPS analyses confirmed the presence of protein layers on the samples. The amount of protein was significant greater for the ion implanted surfaces and was not disrupted upon washing with detergent, hence the formation of strong bonds with the interface was confirmed. While, for non ion implanted surfaces, a decrease of protein was observed after washing with detergent. Finally, the number of stem cells attached to the surface was enhanced for ion implanted surfaces. The studies presented confirm that the developed bionterface with immobilised fibronectin is an effective means to modulate stem cell attachment.
The Faculty of Pharmacy, The University of Sydney, Pharmacy and Bank Building A15, Sydney, NSW, 2006, Australia. wchrzanowski@sydney.edu.au
W
W
Chrzanowski
A
A
Kondyurin
Jae Ho
JH
Lee
Megan S
MS
Lord
M M M
MM
Bilek
Hae-Won
HW
Kim
D000042:Absorption; D000268:Adhesiveness / Q000187:drug effects; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D009532:Nickel / Q000737:chemistry / Q000378:metabolism; D019736:Prostheses and Implants; D011485:Protein Binding; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium / Q000737:chemistry / Q000378:metabolism; D017155:Wettability / Q000187:drug effects
D020099:Coated Materials, Biocompatible; D005353:Fibronectins; C040654:titanium nickelide; D009532:Nickel; D014025:Titanium