Effect of a high-fat challenge on the proteome of human postprandial plasma.
OBJECTIVE
Postprandial lipemia has been associated with inflammation, oxidative stress and vascular dysfunction. This metabolic disturbance represents a complex process only partly understood. The purpose of this study was to identify variations in plasma proteome after a high-fat challenge in healthy middle-aged men.


METHODS
Two-dimensional electrophoresis was used to compare plasma from seven subjects, drawn before and 4 h after a high-fat challenge.


RESULTS
Among the 231 spots detected and analyzed, 22 were present at different levels in postprandial hyperlipemic plasma compared to preprandial plasma. For 10 of them, corresponding proteins were identified by mass spectrometry. Some of them are related to the hemostatic system (tetranectin and fibrinogen) or the complement system (complement component 3 and 4 and ficollin-3) and have been previously associated to atherothrombosis.


CONCLUSIONS
These results provide new perspectives and broaden our understanding of the biological processes of postprandial metabolic stress, as well as its links with the development of atherosclerosis.
INRA, UMR 1019, UNH, CRNH Auvergne, F-63009 Clermont-Ferrand, France.
Nicolas
N
Dejeans
Agnieszka
A
Herosimczyk
Thierry
T
Sayd
Christophe
C
Chambon
Jean-François
JF
Martin
Jeanette A M
JA
Maier
Igor
I
Tauveron
Andrzej
A
Mazur
D000328:Adult; D003176:Complement C3 / Q000378:metabolism; D003181:Complement C4 / Q000378:metabolism; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000008:administration & dosage*; D015180:Electrophoresis, Gel, Two-Dimensional; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D007249:Inflammation / Q000097:blood; D037181:Lectins, C-Type / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D019518:Postprandial Period; D020543:Proteome / Q000378:metabolism*
D003176:Complement C3; D003181:Complement C4; D004041:Dietary Fats; D037181:Lectins, C-Type; D020543:Proteome; C048648:tetranectin; D005340:Fibrinogen