Oxidative stress response in two representative bacteria exposed to atrazine.
Bacteria are present extensively in the environment. Investigation of their antioxidant properties will be useful for further study on atrazine stress tolerance of bacteria and the defense mechanism of antioxidant enzymes against atrazine or other triazine herbicides. Superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and total antioxidant capacity (T-AOC) from one Gram-negative representative strain Escherichia coli K12 and one Gram-positive representative strain Bacillus subtilis B19, respectively, were tested for response to atrazine stress. The results indicated that SOD, CAT, GST and T-AOC were induced upon exposure to atrazine. The growth of two bacteria was better in the absence than in the presence of atrazine, indicating that atrazine can decrease bacterial growth. The changes of enzyme activities indicate the presence of oxidative stress. Oxidative stress induced by atrazine may be due to imbalance of redox potential in bacterial cells, which leads to bacterial metabolic disorder.
School of Resources & the Environment, Northeast Agricultural University, Harbin, China. zhangyinghr@hotmail.com
Ying
Y
Zhang
Dongfang
D
Meng
Zhigang
Z
Wang
Huosheng
H
Guo
Yang
Y
Wang
D000900:Anti-Bacterial Agents / Q000633:toxicity*; D000975:Antioxidants / Q000378:metabolism; D001280:Atrazine / Q000633:toxicity*; D001412:Bacillus subtilis / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D048168:Escherichia coli K12 / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D015870:Gene Expression; D005982:Glutathione Transferase / Q000378:metabolism; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D001280:Atrazine