Serum S-glutathionylated proteins as a potential biomarker of carotid artery stenosis.
OBJECTIVE
As oxidative stress is known to be associated with the development of atherosclerosis, we investigated whether the serum S-glutathionylated proteins were increased in patients with carotid artery stenosis (CS).


METHODS
Fifty-four patients with CS and 20 age-matched non-CS patients were involved in this study. S-glutathionylated proteins in serum were examined by immunoblot analysis using an antibody against S-glutathionylated bovine serum albumin.


RESULTS
The antibody against S-glutathionylated bovine serum albumin was confirmed to specifically recognize the serum S-glutathionylated proteins in patient samples. The S-glutathionylated proteins in serum were significantly increased in the patients with CS (p<0.01) compared to the non-CS patients, and the increase did not depend on the stage of CS. Logistic regression analysis revealed that the serum levels of S-glutathionylated proteins were associated with the development of CS (p<0.01).


CONCLUSIONS
Oxidative stress likely contributes to the development of CS, and serum S-glutathionylated proteins may be a potential biomarker of CS.
Department of Neurosurgery, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
Morito
M
Nakamoto
Makoto
M
Hirose
Miho
M
Kawakatsu
Toshiyuki
T
Nakayama
Yoshishige
Y
Urata
Kensaku
K
Kamata
Makio
M
Kaminogo
Tao-Sheng
TS
Li
Izumi
I
Nagata
D000368:Aged; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000276:immunology / Q000378:metabolism*; D002339:Carotid Arteries / Q000378:metabolism / Q000473:pathology; D016893:Carotid Stenosis / Q000097:blood* / Q000473:pathology; D016022:Case-Control Studies; D002417:Cattle; D005260:Female; D005978:Glutathione / Q000097:blood* / Q000276:immunology; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress
D000906:Antibodies; D015415:Biomarkers; D001798:Blood Proteins; D005978:Glutathione