[The characterization analysis of HAV recombinant antigen was expressed by vaccinia virus vector].
OBJECTIVE
To find a suitable cell line for hepatitis A antigen expressed by vaccinia virus vector and to find a way of inactivation and preservation of the HAV recombinant antigen. Methods Series of cell lines such as K4,143, HEL, Hep-2 and Vero were inoculated with vaccinia virus that can express HAV recombinant antigen. ELISA was used to determine the contents of expression antigen. The characterization of the HAV antigen expressed by vaccinia virus was then analyzed after it was treated with different methods.


RESULTS
The expression of HAV recombinant antigen in K4,143 and HEL cell lines was a little more than expression in Hep-2 and Vero cell lines. The antigenicity is obviously higher when HAV recombinant antigen was inactivated by beta-propiolactone other than it was inactivated by formalin. It was best to preserve the prepared HAV recombinant antigen under -40 degrees C condition.


CONCLUSIONS
The application of vaccinia virus vector in hepatitis A antigen preparation was very useful and promising.
National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
Hong-Lan
HL
Zhao
Fong
F
Qiou
Qing-Ling
QL
Meng
Yao
Y
Y I
Rui-Guang
RG
Tian
Jian
J
Lu
Jing-Yuan
JY
Cao
Sheng-Li
SL
Bi
D000818:Animals; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005557:Formaldehyde / Q000494:pharmacology; D005822:Genetic Vectors; D035921:Hepatitis A Antigens / Q000235:genetics* / Q000276:immunology; D022362:Hepatitis A Vaccines / Q000276:immunology*; D006801:Humans; D011420:Propiolactone / Q000494:pharmacology; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics*
D035921:Hepatitis A Antigens; D022362:Hepatitis A Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D005557:Formaldehyde; D011420:Propiolactone