IgGs containing λ- and κ-type light chains and of all subclasses (IgG1-IgG4) from the sera of patients with autoimmune diseases and viral and bacterial infections hydrolyze DNA.
We present the first evidence demonstrating that small fractions of IgGs of all four subclasses (IgG1-IgG4) from patients with viral (tick-borne encephalitis), bacterial infections (streptococcal infection or erysipelas), and suppurative surgical infections caused by epidermal staphylococci as well as from patients with autoimmune diseases (systemic lupus erythematosus and multiple sclerosis) are catalytically active in the hydrolysis of supercoiled DNA. The hydrolysis of DNA was analyzed by agarose gel electrophoresis. The catalytic activities of nonfractionated IgGs increased in the following order: tick-borne encephalitis < suppurative surgical infection < streptococcal infection < multiple sclerosis < systemic lupus erythematosus, whereas IgGs of healthy donors were inactive. However, the pools of antibodies corresponding to any particular disease were characterized by a specific ratio of IgGs of all four subclasses (IgG1-IgG4) and IgGs containing λ- and κ-type light chains, and each of these subfractions of immunoglobulins demonstrated characteristic relative DNase activity. The relative activities of IgGs containing λ-type light chains may on average be higher, lower, or comparable with those for IgGs with κ-type light chains. The relative contributions of IgGs of different subclasses to the total activity of IgGs also varied widely in the case of various diseases: IgG1 (7%-45%), IgG2 (0.4%-73%), IgG3 (0%-12%), and IgG4 (9%-66%). Thus, immune systems of patients with different diseases can generate a variety of anti-DNA abzymes of different types and with different catalytic properties, which can play an important role in the pathogenesis or protection from the development of these diseases.
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia.
Taisiya A
TA
Parkhomenko
Valentina N
VN
Buneva
Boris M
BM
Doronin
Margarita V
MV
Volkova
Sergey A
SA
Senkovich
Igor I
II
Generalov
Georgy A
GA
Nevinsky
D000293:Adolescent; D000328:Adult; D000368:Aged; D001327:Autoimmune Diseases / Q000097:blood* / Q000276:immunology; D001424:Bacterial Infections / Q000097:blood* / Q000276:immunology; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D006868:Hydrolysis / Q000187:drug effects; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D007145:Immunoglobulin kappa-Chains / Q000097:blood / Q000378:metabolism / Q000494:pharmacology; D007146:Immunoglobulin lambda-Chains / Q000097:blood / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D014777:Virus Diseases / Q000097:blood* / Q000276:immunology; D055815:Young Adult
D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D004247:DNA