Rapid quantitative method for total brominated vegetable oil in soft drinks using ion chromatography.
A simple, quantitative and rapid method for total brominated vegetable oil (BVO) using ion chromatography (IC) with suppressed conductivity detection was developed and successfully applied to soft drinks with results expressed as inorganic bromide anion. The procedure involves extraction of BVO with diethyl ether and treatment with zinc dust in a solution of acetic acid, giving recoveries ranging between 92.5 and 98.5%. The calibration curves obtained were linear with correlation coefficients (r²) of 0.998, a coefficient of variation (CV) of less than 5% and limit of detection (LOD) and limit of quantification (LOQ) of 250 and 750 µg l⁻¹, respectively. The method was successfully applied to the determination of BVO in several commercial soft drinks which were found to contain BVO in the range 1.8-14.510 mg l⁻¹. The method has less sources of error compared to previously published methods.
Food Chemistry Unit, Public Health Laboratories Dep, M.O.H, PO Box 5540 Safatt, Code 13056, Kuwait.
Ashraf A
AA
Yousef
Alaa B
AB
Abbas
Bassam Sh
BSh
Badawi
Wafaa Y
WY
Al-Jowhar
Esam A
EA
Zain
Seham A
SA
El-Mufti
D001628:Beverages / Q000032:analysis / Q000592:standards; D002253:Carbonated Beverages / Q000032:analysis* / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D045703:Emulsifying Agents / Q000032:analysis* / Q000737:chemistry; D005062:European Union; D005503:Food Additives / Q000032:analysis* / Q000737:chemistry; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D057141:Food, Preserved / Q000032:analysis / Q000592:standards; D005638:Fruit / Q000737:chemistry; D054879:Halogenation; D007730:Kuwait; D057230:Limit of Detection; D010938:Plant Oils / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012715:Sesame Oil / Q000032:analysis / Q000737:chemistry
D045703:Emulsifying Agents; D005503:Food Additives; D010938:Plant Oils; D012715:Sesame Oil