Impact of intestinal microbiota on intestinal luminal metabolome.
Low-molecular-weight metabolites produced by intestinal microbiota play a direct role in health and disease. In this study, we analyzed the colonic luminal metabolome using capillary electrophoresis mass spectrometry with time-of-flight (CE-TOFMS) -a novel technique for analyzing and differentially displaying metabolic profiles- in order to clarify the metabolite profiles in the intestinal lumen. CE-TOFMS identified 179 metabolites from the colonic luminal metabolome and 48 metabolites were present in significantly higher concentrations and/or incidence in the germ-free (GF) mice than in the Ex-GF mice (p < 0.05), 77 metabolites were present in significantly lower concentrations and/or incidence in the GF mice than in the Ex-GF mice (p < 0.05), and 56 metabolites showed no differences in the concentration or incidence between GF and Ex-GF mice. These indicate that intestinal microbiota highly influenced the colonic luminal metabolome and a comprehensive understanding of intestinal luminal metabolome is critical for clarifying host-intestinal bacterial interactions.
Dairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd., Tokyo 190-0182, Japan. m-matumoto@meito.co.jp
Mitsuharu
M
Matsumoto
Ryoko
R
Kibe
Takushi
T
Ooga
Yuji
Y
Aiba
Shin
S
Kurihara
Emiko
E
Sawaki
Yasuhiro
Y
Koga
Yoshimi
Y
Benno
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods; D005260:Female; D054884:Host-Pathogen Interactions; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D054892:Metagenome / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013047:Specific Pathogen-Free Organisms