Over-expression of rice leucine-rich repeat protein results in activation of defense response, thereby enhancing resistance to bacterial soft rot in Chinese cabbage.
Pectobacterium carotovorum subsp. carotovorum causes soft rot disease in various plants, including Chinese cabbage. The simple extracellular leucine-rich repeat (eLRR) domain proteins have been implicated in disease resistance. Rice leucine-rich repeat protein (OsLRP), a rice simple eLRR domain protein, is induced by pathogens, phytohormones, and salt. To see whether OsLRP enhances disease resistance to bacterial soft rot, OsLRP was introduced into Chinese cabbage by Agrobacterium-mediated transformation. Two independent transgenic lines over-expressing OsLRP were generated and further analyzed. Transgenic lines over-expressing OsLRP showed enhanced disease resistance to bacterial soft rot compared to non-transgenic control. Bacterial growth was retarded in transgenic lines over-expressing OsLRP compared to non-transgenic controls. We propose that OsLRP confers enhanced resistance to bacterial soft rot. Monitoring expression of defense-associated genes in transgenic lines over-expressing OsLRP, two different glucanases and Brassica rapa polygalacturonase inhibiting protein 2, PDF1 were constitutively activated in transgenic lines compared to non-transgenic control. Taken together, heterologous expression of OsLRP results in the activation of defense response and enhanced resistance to bacterial soft rot.
National Academy of Agricultural Science, RDA, Suwon, 441-707, Korea.
Young Ho
YH
Park
Changhyun
C
Choi
Eun Mi
EM
Park
Hyo Sun
HS
Kim
Hong Jae
HJ
Park
Shin Cheol
SC
Bae
Ilpyung
I
Ahn
Min Gab
MG
Kim
Sang Ryeol
SR
Park
Duk-Ju
DJ
Hwang
D060054:Agrobacterium / Q000235:genetics / Q000378:metabolism; D029741:Brassica rapa / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D000090263:Leucine-Rich Repeat Proteins; D012275:Oryza / Q000235:genetics*; D016973:Pectobacterium carotovorum / Q000472:pathogenicity*; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D011506:Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013312:Stress, Physiological; D014170:Transformation, Genetic; D019076:Transgenes
D000090263:Leucine-Rich Repeat Proteins; D010940:Plant Proteins; D011506:Proteins