Participation of putative glycoside hydrolases SlgC1 and SlgC2 in the biosynthesis of streptolydigin in Streptomyces lydicus.
Two genes of the streptolydigin gene cluster in Streptomyces lydicus cluster encode putative family 16 glycoside hydrolases. Both genes are expressed when streptolydigin is produced. Inactivation of these genes affects streptolydigin production when the microorganism is grown in minimal medium containing either glycerol or d-glucans as carbon source. Streptolydigin yields in S. lydicus were increased by overexpression of either slgC1 or slgC2.
Departamento de Biología Funcional, Universidad de Oviedo, 33006, Oviedo, Spain.
Cristina
C
Gómez
Dina H
DH
Horna
Carlos
C
Olano
Carmen
C
Méndez
José A
JA
Salas
D000617:Aminoglycosides / Q000096:biosynthesis*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D018628:Gene Dosage; D055786:Gene Knockout Techniques; D005936:Glucans / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*
D000617:Aminoglycosides; D003470:Culture Media; D005936:Glucans; C100307:streptolydigin; D002244:Carbon; D006026:Glycoside Hydrolases; D005990:Glycerol