Diversification of IFNγ-inducible CXCb chemokines in cyprinid fish.
We earlier identified two CXCL8-like lineages in cyprinid fish, which are functional homologues of the mammalian CXCL8, but with diverged functions. We here investigated whether the carp IFN-γ-inducible CXCb gene, related to the mammalian CXCL9, -10 and -11 chemokines, was subject to a similar diversification. On the zebrafish genome, a cluster of seven CXCb genes was found on chromosome five. Analysis of the promoter of the zebrafish CXCb genes suggests a partially shared, but differential induction. A second CXCb gene, CXCb2, was identified in common carp by homology cloning. CXCb2 is constitutively expressed in immune-related tissues, predominantly in head kidney lymphocytes/monocytes. Interestingly, an induction of CXCb2 gene expression with recombinant carp IFN-γ2 and LPS was observed in macrophages and granulocytes. Finally, difference in sensitivity to LPS, and kinetics of CXCb1 and CXCb2 gene expression during zymosan-induced peritonitis, was observed. These results indicate a functional diversification for cyprinid CXCb chemokines, with functional homology to mammalian CXCL9-11.
Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH, Wageningen, The Netherlands.
Lieke M
LM
van der Aa
Magdalena
M
Chadzinska
Wouter
W
Derks
Marleen
M
Scheer
Jean-Pierre
JP
Levraud
Pierre
P
Boudinot
B M
BM
Lidy Verburg-van Kemenade
D000818:Animals; D002347:Carps / Q000276:immunology* / Q000378:metabolism; D019743:Chemokines, CXC / Q000235:genetics*; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism*; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D010537:Peritoneum / Q000166:cytology / Q000276:immunology; D010538:Peritonitis / Q000276:immunology; D011401:Promoter Regions, Genetic; D059467:Transcriptome; D015027:Zebrafish / Q000235:genetics / Q000276:immunology; D015054:Zymosan
D019743:Chemokines, CXC; D008070:Lipopolysaccharides; D007371:Interferon-gamma; D015054:Zymosan