Imaging histamine in live basophils and macrophages with a fluorescent mesoionic acid fluoride.
Histamine is a biogenic amine with fundamental roles in circulatory and immune systems. We report a fluorescent small molecule (Histamine Blue) for imaging intracellular histamine in live basophils and macrophages. Histamine Blue is a fluorescent mesoionic acid fluoride that turns on upon reaction with histamine. The selective response of Histamine Blue enabled the visualization of intracellular histamine under different physiological conditions.
Institute for Biomedical Research, Barcelona Science Park, Spain.
Nicola
N
Kielland
Marc
M
Vendrell
Rodolfo
R
Lavilla
Young-Tae
YT
Chang
D000818:Animals; D001491:Basophils / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging; D005456:Fluorescent Dyes / Q000378:metabolism*; D005459:Fluorides / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D051381:Rats
D005456:Fluorescent Dyes; D006632:Histamine; D005459:Fluorides