Effects on inflammatory responses by the sphingoid base 4,8-sphingadienine.
Sphingolipids (SLs) are a class of lipids, which are structural cell components             involved in the regulation of cellular processes such as cell proliferation, differentiation,             apoptosis and inflammation. Dietary SLs are enzymatically hydrolyzed in the gut             lumen into metabolites, namely ceramides and sphingoid bases. The sphingoid base             4,8-sphingadienine (4,8-SD) is the metabolite of glucocerebrosides derived from             plants that are part of the human diet. The present findings provide insight into             the effects of 4,8-SD on inflammatory responses that may be of nutritional and             therapeutic benefit. We demonstrated that 4,8-SD significantly inhibited tumor             necrosis factor-α (TNF-α)- and lipopolysaccharide (LPS)-induced expression of             IL-8 and E-selectin in human endothelial cells in a dose-dependent manner. The             anti-inflammatory effect was observed at significantly lower concentrations of             4,8-SD compared those affecting cell viability as judged by the LDH and WST-1             assays.
Department of Pharmacognosy, University of Vienna, A-1090 Vienna,                     Austria.
Evelien
E
Rozema
Markus
M
Binder
Murty
M
Bulusu
Valery
V
Bochkov
Georg
G
Krupitza
Brigitte
B
Kopp
D031057:Arisaema / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D019040:E-Selectin / Q000378:metabolism; D004983:Ethanolamines / Q000737:chemistry / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D019040:E-Selectin; D004983:Ethanolamines; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; C003337:sphingadienine