Aptasensing of chloramphenicol in the presence of its analogues: reaching the maximum residue limit.
A novel, label-free folding induced aptamer-based electrochemical biosensor for the detection of chloramphenicol (CAP) in the presence of its analogues has been developed. CAP is a broad-spectrum antibiotic that has lost its favor due to its serious adverse toxic effects on human health. Aptamers are artificial nucleic acid ligands (ssDNA or RNA) able to specifically recognize a target such as CAP. In this article, the aptamers are fixed onto a gold electrode surface by a self-assembly approach. In the presence of CAP, the unfolded ssDNA on the electrode surface changes to a hairpin structure, bringing the target molecules close to the surface and triggering electron transfer. Detection limits were determined to be 1.6 × 10(-9) mol L(-1). In addition, thiamphenicol (TAP) and florfenicol (FF), antibiotics with a structure similar to CAP, did not influence the performance of the aptasensor, suggesting a good selectivity of the CAP-aptasensor. Its simplicity and low detection limit (because of the home-selected aptamers) suggest that the electrochemical aptasensor is suitable for practical use in the detection of CAP in milk samples.
Department of Chemistry, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.
Sanaz
S
Pilehvar
Jaytry
J
Mehta
Freddy
F
Dardenne
Johan
J
Robbens
Ronny
R
Blust
Karolien
K
De Wael
D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D002417:Cattle; D002701:Chloramphenicol / Q000031:analogs & derivatives / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D008892:Milk / Q000737:chemistry; D013839:Thiamphenicol / Q000031:analogs & derivatives / Q000737:chemistry
D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D002701:Chloramphenicol; D006046:Gold; C035534:florfenicol; D013839:Thiamphenicol