Transgenic nude mouse with green fluorescent protein expression-based human glioblastoma multiforme animal model with EGFR expression and invasiveness.
Previously, we developed an orthotopic xenograft model of human glioblastoma multiforme (GBM) with high EGFR expression and invasiveness in Balb/c nu/nu nude mice. Now we also developed the same orthotopic xenograft model in transgenic nude mice with green fluorescent protein (GFP) expression. The present orthotopic xenografts labeled by phycoerythrin fluorescing red showed high EGFR expression profile, and invasive behavior under a bright green-red dual-color fluorescence background. A striking advantage in the present human GBM model is that the change of tumor growth can be observed visually instead of sacrificing animals in our further antitumor therapy studies.
School of Life Sciences, Neurosurgical Department of the First Affiliated Hospital, Xiamen University, China.
Guo-Wei
GW
Tan
Fo-Lin
FL
Lan
Jian-Guo
JG
Gao
Cai-Mou
CM
Jiang
Yi
Y
Zhang
Xiao-Hong
XH
Huang
Yue-Hong
YH
Ma
He-Dui
HD
Shao
Xue-Yang
XY
He
Jin-Long
JL
Chen
Jian-Wu
JW
Long
Hui-Sheng
HS
Xiao
Zhi-Tong
ZT
Guo
Yi
Y
Diao
D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D018773:Genes, erbB-1; D005909:Glioblastoma / Q000098:blood supply / Q000235:genetics* / Q000473:pathology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
D049452:Green Fluorescent Proteins