Development of microsatellite markers in a riparian shrub, Spiraea thunbergii (Rosaceae).
OBJECTIVE
Microsatellite primers in the deciduous shrub Spiraea thunbergii were developed to investigate genetic diversity and population genetic structure. Cross-species transferability was assayed in four congeneric species.


RESULTS
Using a compound simple sequence repeat (SSR) marker method, 10 primer sets were identified in Japanese populations of S. thunbergii. The primers amplified compound SSRs with two to five alleles per locus. More than half of the primers were also amplified in S. prunifolia, S. nipponica var. nipponica, and S. japonica.


CONCLUSIONS
These markers might be useful for future studies of population genetics of S. thunbergii and congeneric species.
Organization for the Strategic Coordination of Research and Intellectual Property, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa, Japan.
Kazuya
K
Ashizawa
Megumi K
MK
Kimura
Akiko
A
Takahashi
Chunlan
C
Lian
Noboru
N
Kuramoto
D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population / Q000379:methods; D018895:Microsatellite Repeats; D045483:Rivers; D013045:Species Specificity; D031992:Spiraea / Q000235:genetics*
D018744:DNA, Plant; D005819:Genetic Markers