A sensitive dual signal amplification method for western blotting based on antibody-functionalised graphene oxide and gold nanoparticles.
In this study, we report an ultrasensitive western blotting method using antibody-functionalised graphene oxide sheets and gold nanoparticles. Additionally, the cost is reduced greatly by conjugating two different primary antibodies on gold nanoparticles (C. Welinder and L. Ekblad, J. Proteome Res., 2011, 10, 1416-1419).
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China.
Hongjun
H
Lin
Jingrui
J
Huo
Aihong
A
Zhang
Yingfu
Y
Liu
Qingming
Q
Wang
Yun
Y
Cai
Wantao
W
Ying
Weijie
W
Qin
Yangjun
Y
Zhang
Xiaohong
X
Qian
D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D015153:Blotting, Western / Q000191:economics / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D016271:Proto-Oncogene Proteins c-myc / Q000276:immunology
D000906:Antibodies; D010087:Oxides; D016271:Proto-Oncogene Proteins c-myc; D006046:Gold; D006108:Graphite