Transgenic expression of Tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum.
Plant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic tobacco expressing a variant of Tobacco mosaic virus (TMV) coat protein (CP(T42W)) or movement protein (MP), and a hybrid line (MP×CP(T42W)) that coexpresses both proteins, to study the plant response to individual viral proteins. Findings employing microarray analysis of MP×CP(T42W) plants and silenced mp×cp(T42W)* controls revealed that altered transcripts were mostly downregulated, suggesting a persistent shut-off due to MP×CP(T42W) expression. Next, we showed that MP triggered reactive oxygen species (ROS) accumulation, reduction of total ascorbate, and expression of ROS scavenging genes. These effects were enhanced when both proteins were coexpressed. MP and MP×CP(T42W) plants showed increased levels of salicylic acid (SA) and SA-responsive gene expression. Furthermore, these effects were partially reproduced in Nicotiana benthamiana when GMP1 transcript was silenced. CP(T42W) seems to be playing a negative role in the defense response by reducing the expression of PR-1 and RDR-1. MP and MP×CP(T42W) transgenic expression promoted a recovery-like phenotype in TMV RNA infections and enhanced susceptibility to Pseudomonas syringae and Sclerotinia sclerotiorum. The individual effects of viral proteins may reflect the ability of a virus to balance its own virulence.
Instituto de Biotecnologia, CICVyA-INTA, Hurlingham, Buenos Aires, Argentina.
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Conti
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Rodriguez
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Manacorda
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Asurmendi
D001205:Ascorbic Acid / Q000378:metabolism; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D020868:Gene Silencing; D010084:Oxidation-Reduction; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000821:virology; D053830:Plant Viral Movement Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
D036022:Capsid Proteins; D016166:Free Radical Scavengers; D053830:Plant Viral Movement Proteins; D017382:Reactive Oxygen Species; D001205:Ascorbic Acid