Fiber-based source for multiplex-CARS microscopy based on degenerate four-wave mixing.
We present a fiber-based laser source for multiplex coherent anti-Stokes Raman scattering (CARS) microscopy. This source is very compact and potentially alignment-free. The corresponding pump and Stokes pulses for the CARS process are generated by degenerate four-wave mixing (FWM) in photonic-crystal fibers. In addition, an ytterbium-doped fiber laser emitting spectrally narrow 100 ps pulses at 1035 nm wavelength serves as pump for the FWM frequency conversion. The FWM process delivers narrow-band pulses at 648 nm and drives a continuum-like spectrum ranging from 700 to 820 nm. With the presented source vibrational resonances with energies between 1200 cm-1and 3200 cm-1can be accessed with a resolution of 10 cm-1. Additionally, the temporal characteristics of the FWM output have been investigated by a cross-correlation setup, revealing the suitability of the emitted pulses for CARS microscopy. This work marks a significant step towards a simple and powerful all-fiber, maintenance-free multiplex-CARS source for real-world applications outside a laboratory environment.
Institute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-Universität Jena, Max-Wien-Platz 1, 07743 Jena, Germany. Thomas.Gottschall@uni-jena.de
Thomas
T
Gottschall
Martin
M
Baumgartl
Aude
A
Sagnier
Jan
J
Rothhardt
Cesar
C
Jauregui
Jens
J
Limpert
Andreas
A
Tünnermann
D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation*; D007834:Lasers; D008853:Microscopy / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*