Effect of neonatal exposure on male rats to bisphenol A on the expression of DNA methylation machinery in the postimplantation embryo.
Bisphenol A (BPA) is used in the production of polycarbonate plastics and epoxy resins. Our previous studies have demonstrated that neonatal exposure of male rats to BPA causes decrease in sperm count and motility, increase in postimplantation loss (POL), ultimately leading to subfertility during adulthood. Epigenetic mechanisms such as DNA methylation play an important role in embryo development. DNA methyltransferases (Dnmts) are the key players involved in regulating DNA methylation marks. The objective of the present study was to determine the mechanism involved in resorption of embryo as a result of BPA exposure. The results of the present study demonstrate that neonatal exposure of male rats to BPA down regulates the gene expression of Dnmts and related transcription factors in resorbed embryos as compared with the viable embryo. Thereby, suggesting that BPA may have altered the sperm epigenome, which might have affected the embryo development and leading to an increase in the POL.
National Center for Preclinical Reproductive and Genetic Toxicology, National Institute for Research in Reproductive Health (ICMR), Mumbai, Maharashtra, India.
Tanvi
T
Doshi
Criselle
C
D'Souza
Vikas
V
Dighe
Geeta
G
Vanage
D000818:Animals; D000831:Animals, Newborn; D001559:Benzhydryl Compounds / Q000633:toxicity*; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation / Q000187:drug effects*; D000090205:DNA Methyltransferase 3A; D020964:Embryo Loss / Q000139:chemically induced*; D047108:Embryonic Development / Q000187:drug effects*; D052244:Endocrine Disruptors / Q000633:toxicity*; D044127:Epigenesis, Genetic; D005260:Female; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects; D007248:Infertility, Male / Q000139:chemically induced; D008297:Male; D009845:Oligospermia / Q000139:chemically induced; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D051705:Sp3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D013081:Sperm Motility / Q000187:drug effects; D014158:Transcription, Genetic; D000097584:DNA Methyltransferase 3B; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; D052244:Endocrine Disruptors; D010636:Phenols; D016329:Sp1 Transcription Factor; D051705:Sp3 Transcription Factor; D000097584:DNA Methyltransferase 3B; C497123:Sp3 protein, rat; C006780:bisphenol A; D000098826:Bisphenol A Compounds