Forced exercise changes catecholamine synthesis in the spleen of adult rats.
Treadmill training produces modulation of neuro-endocrine and immune functions. This study examined the effects of chronic forced running (CFR) on the plasma concentration of catecholamines and the expression of splenic catecholamine biosynthetic enzymes in rats by using real-time RT-PCR and Western blot analyses. We found that CFR increases the plasma catecholamine levels, decreases splenic tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) mRNA levels and increases splenic PNMT protein levels. This shows that CFR is a very strong stressor which activates the sympatho-adrenomedullary system and increases synthesis of splenic PNMT by 20%, which both can modulate the immune function.
Institute of Nuclear Sciences Vinča, Laboratory of Molecular Biology and Endocrinology, University of Belgrade, Belgrade, Serbia. gljubica@vinca.rs
Ljubica
L
Gavrilović
Vesna
V
Stojiljković
Jelena
J
Kasapović
Snežana
S
Pejić
Ana
A
Todorović
Snežana B
SB
Pajović
Sladjana
S
Dronjak
D000818:Animals; D002395:Catecholamines / Q000096:biosynthesis* / Q000097:blood; D004299:Dopamine beta-Hydroxylase / Q000096:biosynthesis; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D010625:Phenylethanolamine N-Methyltransferase / Q000096:biosynthesis; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012420:Running / Q000502:physiology; D013154:Spleen / Q000378:metabolism*; D013312:Stress, Physiological; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis
D002395:Catecholamines; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D010625:Phenylethanolamine N-Methyltransferase