[Effects of external use of jiuyi dan for one month on blood and urine mercury levels and liver and kidney functions of rabbits].
OBJECTIVE
To observe the changes of the blood and urine mercury (Hg) levels and liver & kidney functions of rabbits after administration of Jiuyi Dan (calcined gypsum-Sheng Dan 9: 1) for 1 month and the recovery of rabbits after the drug withdrawal.


METHODS
The rabbits were randomly divided into 2 groups: the calcined gypsum group and the Jiuyi Dan group. After 36 mg of calcined gypsum and 40 mg of Jiuyi Dan were used on the surface of wound (5 cm x 5 cm) on one side of rabbit back for 4 h, the surfaces of wound were washed by saline. The bloods were taken from the rabbit hearts before and after the drug administration for 14 and 28 days, and after the drug withdrawal for 7, 40, 71, and 92 days for determining Hg level in blood, and liver & kidney function indicators (ALT, AST, CREAT and BUN). The Hg level in urine collected from bladders was examined while rabbits were dissected after the drug withdrawal for 1, 40, 71, and 92 days.


RESULTS
The Hg level in blood was significantly increased (P < 0.01) after the rabbits were administrated with drugs for 14 and 28 days and after the drug treatment was stopped for 7 and 40 days. The Hg level in urine was significantly enhanced after the drug withdrawal for 1, 40, 71 days. However, the liver & kidney indicators were not influenced.


CONCLUSIONS
The Hg level in rabbit blood and urine was significantly increased after the consecutive administration of double-dose Jiuyi Dan for 1 month. However, the blood Hg level and urine Hg level recover after the drug withdrawal for 71 days and 3 months, respectively. The liver & kidney indicators do not significantly change with the dose.
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Yu'e
Y
Cao
Xiaomiao
X
Chen
Shengguang
S
Fu
Meina
M
Ye
Xiwen
X
Jin
Ruomin
R
Jin
Hongfeng
H
Chen
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001806:Blood Urea Nitrogen; D001835:Body Weight / Q000187:drug effects; D003404:Creatinine / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000633:toxicity*; D005260:Female; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008628:Mercury / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011817:Rabbits; D011897:Random Allocation; D012867:Skin / Q000187:drug effects* / Q000293:injuries; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D003404:Creatinine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D008628:Mercury