Influence of large molecular polymeric pigments isolated from fermented Zijuan tea on the activity of key enzymes involved in lipid metabolism in rat.
Influence of large molecular polymeric pigments (LMPP) isolated from fermented Zijuan tea on the activity and mRNA expression of key enzymes involved in lipid metabolism in rat was explored. The results show that intragastric infusion of high-dose LMPP (1.215 g/kg body weight) effectively suppressed the elevation in TC and LDL-C (p<0.05), and prevented the reduction in HDL-C (p<0.05), compared with the hyperlipidemia model group. LMPP significantly enhanced the activity of HL and HSL, and increased the HSL mRNA expression in the liver tissue and adipose tissue. High-LMPP treatment significantly reduced the HMG-CoA reductase expression by 56.5% in the liver compared with hyperlipidemia model group. In contrast, LDL-R expression was increased by 120% in the presence of high-LMPP treatment. These results suggest that LMPP have the hypolipidemic effect to some extent and significantly enhance HSL mRNA expression in the liver and adipose tissue, thereby increasing HSL activity in rat.
Faculty of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan 650201, China.
Qiu-Ping
QP
Wang
Chun-Xiu
CX
Peng
Bin
B
Gao
Jia-Shun
JS
Gong
D000214:Acyl Coenzyme A / Q000187:drug effects; D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D004798:Enzymes / Q000187:drug effects*; D005285:Fermentation; D006949:Hyperlipidemias / Q000188:drug therapy / Q000201:enzymology; D050356:Lipid Metabolism / Q000187:drug effects*; D008071:Lipoprotein Lipase / Q000187:drug effects; D008099:Liver / Q000201:enzymology; D008297:Male; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000187:drug effects; D010860:Pigments, Biological / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002787:Sterol Esterase / Q000187:drug effects; D013662:Tea
D000214:Acyl Coenzyme A; D004798:Enzymes; D010860:Pigments, Biological; D010936:Plant Extracts; D012333:RNA, Messenger; D013662:Tea; C008047:3-hydroxy-3-methylglutaryl-coenzyme A; D002784:Cholesterol; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D002787:Sterol Esterase; D008071:Lipoprotein Lipase