Identification and biological activities of bryostatins from Japanese bryozoan.
Six bryostatins were isolated from Japanese bryozoan by evaluating their binding to the C1B domain of protein kinase Cδ (PKCδ). Structure-activity studies of bryostatins 4, 10, and 14 suggested that the ester group at C20 was not necessary for binding to and activating PKCδ. These bryostatins showed significant anti-tumor-promoting activity in induction tests with the Epstein-Barr virus early antigen.
Department of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Japan.
Sayo
S
Ueno
Ryo C
RC
Yanagita
Kazuma
K
Murakami
Akira
A
Murakami
Harukuni
H
Tokuda
Nobutaka
N
Suzuki
Takeshi
T
Fujiwara
Kazuhiro
K
Irie
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000821:virology; D054713:Bryostatins / Q000302:isolation & purification* / Q000494:pharmacology; D002013:Bryozoa / Q000737:chemistry*; D004789:Enzyme Activation; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D007564:Japan; D011485:Protein Binding; D051745:Protein Kinase C-delta / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship
D000956:Antigens, Viral; D000970:Antineoplastic Agents; D054713:Bryostatins; C033305:Epstein-Barr virus early antigen; D007527:Isoenzymes; D051745:Protein Kinase C-delta