Sequencing and phylogenetic analysis of the hexon, fiber, and penton regions of adenoviruses isolated from AIDS patients.
Sequencing and phylogenetic analysis of the hexon, fiber, and penton regions of adenoviruses isolated between 1986 and 1997 from AIDS patients has been performed. Sequencing the L2 part of the hexon gene of 51 adenoviruses isolated between 1986 and 1997 from AIDS patients revealed only one type each from species A and C and two types from species B with all the remaining isolates from species D. Further sequencing and phylogenetic analysis of the fiber knob region of these species D adenoviruses revealed that 28/46 were intermediate strains with conflicting hexon and fiber sequences. When the penton regions of these intermediate strains were sequenced, it became clear that some had originated from a third adenovirus type presumably by intergene recombination events. Evidence from sequencing the L1 hexon and fiber shaft regions showed no evidence of intragene recombination but penton sequences showed that recombination between the hypervariable region (HVR) and RGD regions was common. Six isolates appear to be from three new adenovirus types. Five AIDS patients showed sequential infection with different adenovirus variants and six such variants were isolated from a single patient in 2 years.
Virology Unit, Inflammation Sciences Group, The University of Manchester, Manchester, United Kingdom.
Yasir Mohammed A
YM
Al Qurashi
Moustafa Alissa
MA
Alkhalaf
Lusiana
L
Lim
Malcolm
M
Guiver
Robert J
RJ
Cooper
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000821:virology; D000257:Adenoviridae Infections / Q000150:complications* / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D015658:HIV Infections / Q000150:complications* / Q000821:virology; D006801:Humans; D008297:Male; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus; C043322:penton protein, adenovirus