[Expression of c-myc and c-myb gene in leukemic bone marrow stromal cells and their correlation].
This study was purposed to investigate the expression level of oncogene c-myc and c-myb in leukemic cells and leukemic bone marrow stromal cells (BMSC) and the correlation with each other. The expression levels of c-myc and c-myb in those cells were examined semi-quantitatively by reverse transcription polymerase chain reaction (RT-PCR). Flow cytometry analysis was used to detect the membrane surface antigens of leukemic cells and BMSC. The karyotype was analyzed by G-banding techniques. The results showed that (1) c-myc and c-myb gene were expressed in the normal control group, the leukemic cells and BMSC of patients group. The mean expression levels of c-myb mRNA and c-myc mRNA in abnormal chromosomal leukemic cells were 1.03 ± 0.48 and 1.15 ± 0.38 respectively, which were significantly higher than those in control group (P < 0.05). In the abnormal karyotype stromal cells, the mean expression level of c-myb mRNA and c-myc mRNA were 2.08 ± 0.82 and 1.46 ± 0.29 respectively (P < 0.05). (2) The expression level of c-myc and c-myb mRNA were closely associated with patients' platelet counts (P < 0.05). (3) The expression of c-myc mRNA linearly correlated with the expression of c-myb mRNA in different prognostic groups. (4) In acute leukemic cells and BMSC, c-myc expression positively correlated with c-myb expression. (5) The expression level of c-myc in leukemic cells correlated with the expression levels of c-myc and c-myb in BMSC, respectively. It is concluded that the reduction of c-myc or c-myb expression levels may be a therapeutic regimen for leukemia.
The First Hospital of Lanzhou University, Lanzhou, Gansu Province, China.
Ying
Y
Zhang
Rui
R
Chen
Wei
W
Zhang
Juan
J
Li
Xuan
X
Chen
Li
L
Zhao
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D015973:Gene Expression Regulation, Leukemic; D020570:Genes, myb; D016259:Genes, myc; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008875:Middle Aged; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
C489427:MYC protein, human; D020598:Proto-Oncogene Proteins c-myb; D016271:Proto-Oncogene Proteins c-myc