[Expression, purification and identification of St. Louis encephalitis virus-like particles in eukaryotic cells].
OBJECTIVE
To express St. Louis encephalitis virus-like particles in mammalian cells, it will provide a prerequisite for further immunological diagnostic studies.


METHODS
293T-cell were transiently transfected with recombinant PreM-E plasmid. Expression and antigenicity of the purified protein were determined by transmission electron microscope (TEM), Western-Blot, immunofluorescence assay and ELISA.


RESULTS
Recombinant subviral particles, about 50 nm in diameter, were observed by TEM in the supernatant of transfected cells. The results of Western-Blot, IFA and ELISA showed the recombinant proteins retained immunoreactivity similar to those of native virus proteins.


CONCLUSIONS
St. Louis encephalitis virus-like particles has good antigenicity and physical appearance. It will provide a prerequisite for further immune diagnostic reagent.
Changchun Institute of Biological products, Changchun 130000, China.
Xue
X
Guo
Fu-Bin
FB
Xing
Qing-Jie
QJ
Xian
Lin
L
Wang
Peng-Fei
PF
Yang
Li-Ping
LP
Zhang
Rui-Jin
RJ
Ping
Kong-Xin
KX
Hu
D004665:Encephalitis Virus, St. Louis / Q000276:immunology*; D004674:Encephalitis, St. Louis / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D057809:HEK293 Cells; D006801:Humans; D010957:Plasmids; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D014162:Transfection; D014771:Virion / Q000276:immunology* / Q000302:isolation & purification
D011994:Recombinant Proteins