Identification of Enzyme Inhibitors Using Therapeutic Target Protein - Magnetic Nanoparticle Conjugates.
Target protein - magnetic nanoparticle (MNP) conjugates, i.e. α-glucosidase-MNP and protein tyrosine phosphatase 1B (PTP1B)-MNP, were prepared and evaluated for the first time for affinity extraction of the enzyme inhibitors from herbal extracts. Four ligands extracted from granati pericarpium were identified by ESI-MS analysis. In vitro tests indicated that they inhibited both α-glucosidase and PTP1B, two important target proteins for diabetic treatment.
National Engineering Research Center for Natural Medicines, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, P.R. China. Tel: 86-28-85253968.
Lin-Sen
LS
Qing
Nan
N
Tang
Ying
Y
Xue
Jian
J
Liang
Yi-Ming
YM
Liu
Xun
X
Liao