[Immunomodulatory effects of Opuntia dillenii polysaccharides on specific immune function of mice].
OBJECTIVE
To investigate specific immune modulation of Opuntia dillenii polysaccharides (ODPs) on mice (body fluid and cells).


METHODS
An immunosuppressed murine model was induced by intraperitoneal injection of cyclophosphamide (Cy, ip). The intraserous hemolysin IgM, IgG levels, the proliferation of spenocytes and the proportion of T lymphocyte subsets in peripheral blood were determined after the mice treated by ODPs. Proliferative effects of ODP-I, the main component purified from ODPs, on the lymphocytes in vitro were also studied.


RESULTS
ODPs could significantly increase intraserous IgM and IgG levels, significantly enhance the proliferation of T and B lymphocytes and restore them to normal level. ODPs significantly reduced the percentage of CD4+ T lymphocytes subset, therefore significantly reduced the proportion of CD4+ and CD8+ T lymphocytes of peripheral blood from immunosuppressive mice and restored it to normal level. ODP-I significantly enhanced the natural proliferation of splenic lymphocyte and assisted the proliferation of T and B lymphocytes which induced by mitogen ConA or LPS.


CONCLUSIONS
ODPs can enhance the specific immune function of immunosuppressed mice and the proliferation of lymphocytes in vitro.
College of Life Science and Technology, Zhanjiang Normal University, Zhanjiang 524048, China. zhaolongyan-dra@163.com
Long-Yan
LY
Zhao
Song-Lian
SL
Zhang
Qing-Xia
QX
Yuan
Jie
J
Cheng
Fu-Hua
FH
Zeng
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000008:administration & dosage; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D016867:Immunocompromised Host / Q000187:drug effects*; D007136:Immunoglobulins / Q000096:biosynthesis / Q000097:blood; D007165:Immunosuppression Therapy; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D029747:Opuntia / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D013154:Spleen / Q000187:drug effects / Q000276:immunology
D000276:Adjuvants, Immunologic; D007136:Immunoglobulins; D011134:Polysaccharides; D003520:Cyclophosphamide