Evaluation of injection conditions for preparative supercritical fluid chromatography.
Preparative supercritical fluid chromatography (SFC) has become the preferred method for the rapid purification of drug candidates during the pharmaceutical discovery process. This paper will discuss the evaluation of injection techniques for preparative SFC. A thorough evaluation of mixed stream vs. modifier stream™ injection was performed. It was shown that for the majority of the compounds evaluated, modifier stream injection gave better resolution relative to mixed stream injection. The improvement in resolution with modifier stream injection increased as injection volume increased. In addition, a study evaluating the effect of dissolution solvent on chromatographic performance for the preparative resolution of enantiomers using SFC showed that dissolution solvent had minimal impact on preparative resolution for the preparative SFC separation of trans stilbene oxide.
Amgen, 360 Binney Street, Cambridge, MA 02142, USA. millerl@amgen.com
Larry
L
Miller
Ian
I
Sebastian
D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D008956:Models, Chemical; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification; D013237:Stereoisomerism; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification
D004364:Pharmaceutical Preparations; D013267:Stilbenes; C025906:stilbene oxide