[Study on stability of major pharmaceutical ingredients of vladimiriae radix before and after being roasted in artificial gastric juice, artificial intestinal juice and isolated rat gastric, intestinal or colonic incubation juice].
OBJECTIVE
To study the stability of costunolide (COS) and dehydrocostus lactone (DEH) of Vladimiriae Radix before and after being roasted in artificial gastric juice, artificial intestinal juice and isolated rat gastric, intestinal or colonic incubation juice.


METHODS
The HPLC method was used for the determination of the mass concentration of COS and DEH Vladimiriae Radix before and after being roasted artificial gastric juice, artificial intestinal juice and isolated rat gastric, intestinal or colonic incubation juice. The samples were incubated with isolated rat stomach, small intestine; colon was used to study physical adsorption, absorption or degradation parameters.


RESULTS
COS of Vladimiriae Radix before or after being roasted was unstable in artificial gastric juice, with the average degradation constants as 0.758 0 and 0.531 1. Having been roasted, it showed an increasing stability with a significant difference (P < 0.01). Both of COS and DEH of Vladimiriae Radix before or after being roasted showed high adsorption, uptake or degradation (2 h), and it had significant difference between different parts.


CONCLUSIONS
COS was unstable in artificial gastric juice (unprocessed Vladimiriae Radix has a higher degradation rate). Isolated rat stomach, small intestine, colon can adsorb, take, degrade COS and DEH of Vladimiriae Radix before or after roasting process obviously and differently. It provides basis for studies on the absorption mechanisms of effective ingredients of Vladimiriae Radix before and after being roasted.
School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Chengdu 611137, China. 12724591@qq.com
Qian
Q
Mao
Chaomei
C
Fu
Huiling
H
Hu
Zhanguo
Z
Wang
Yao
Y
He
Xifu
X
Bao
D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000378:metabolism; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D005750:Gastric Juice / Q000737:chemistry; D005753:Gastric Mucosa / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007419:Intestinal Secretions / Q000737:chemistry; D007783:Lactones / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012717:Sesquiterpenes / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics
D004365:Drugs, Chinese Herbal; D007783:Lactones; D012717:Sesquiterpenes; C083030:dehydrocostus lactone; C002602:costunolide