The first discovered water channel protein, later called aquaporin 1: molecular characteristics, functions and medical implications.
After a decade of work on the water permeability of red blood cells (RBC) Benga group in Cluj-Napoca, Romania, discovered in 1985 the first water channel protein in the RBC membrane. The discovery was reported in publications in 1986 and reviewed in subsequent years. The same protein was purified by chance by Agre group in Baltimore, USA, in 1988, who called in 1991 the protein CHIP28 (CHannel forming Integral membrane Protein of 28 kDa), suggesting that it may play a role in linkage of the membrane skeleton to the lipid bilayer. In 1992 the Agre group identified CHIP28's water transport property. One year later CHIP28 was named aquaporin 1, abbreviated as AQP1. In this review the molecular structure-function relationships of AQP1 are presented. In the natural or model membranes AQP1 is in the form of a homotetramer, however, each monomer has an independent water channel (pore). The three-dimensional structure of AQP1 is described, with a detailed description of the channel (pore), the molecular mechanisms of permeation through the channel of water molecules and exclusion of protons. The permeability of the pore to gases (CO(2), NH(3), NO, O(2)) and ions is also mentioned. I have also reviewed the functional roles and medical implications of AQP1 expressed in various organs and cells (microvascular endothelial cells, kidney, central nervous system, eye, lacrimal and salivary glands, respiratory apparatus, gastrointestinal tract, hepatobiliary compartments, female and male reproductive system, inner ear, skin). The role of AQP1 in cell migration and angiogenesis in relation with cancer, the genetics of AQP1 and mutations in human subjects are also mentioned. The role of AQP1 in red blood cells is discussed based on our comparative studies of water permeability in over 30 species.
First Laboratory of Genetic Explorations, Cluj County Clinical Emergency Hospital, Cluj-Napoca, Romania. gbgbenga@gmail.com
Gheorghe
G
Benga
D000818:Animals; D051398:Aquaporin 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002463:Cell Membrane Permeability; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D005740:Gases / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008659:Metabolic Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008958:Models, Molecular; D009154:Mutation; D055503:Protein Multimerization; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014867:Water / Q000378:metabolism*
D005740:Gases; D021122:Protein Subunits; D014867:Water; D051398:Aquaporin 1