One-cell biopsy significantly improves the outcome of preimplantation genetic diagnosis (PGD) treatment: retrospective analysis of 569 PGD cycles at the Stockholm PGD centre.
OBJECTIVE
What are the significant factors that influence the outcome of a PGD treatment?


CONCLUSIONS
The age of the woman and the number of biopsied cells per embryo are of significant importance for a successful PGD treatment.


BACKGROUND
Younger women are more likely to succeed with an IVF treatment.


METHODS
Cohort study, retrospective analysis of 569 PGD cycles, 1996-2009.


METHODS
256 couples and 569 PGD treatments at 'Stockholm PGD centre'. At this centre after 2003, a 1-cell policy was applied, when possible, with respect to the reliability of the diagnostic test and since 2009, 1-cell biopsy policy was also applied for monogenic disorders.


RESULTS
The women under 36 years of age were three times more likely to get pregnant after PGD treatment, P = 0.003 and odds ratio 3.1 [95% confidence interval (CI) 1.5-6.5]. The 1-cell biopsy cycles were twice as likely to result in a pregnancy in comparison with cycles were 2 cells were removed from the embryo, P = 0.0013 and odds ratio 2.55 (95% CI 1.44-4.52). No other factors were found to be significant for the outcome.


CONCLUSIONS
Retrospective analysis with 1- and 2-cell biopsies at different times.


CONCLUSIONS
The results will have an impact on the implementation of PGD in general, thereby making it possible to significantly improve the treatment outcome.
Clinical Genetics Unit, Department of Molecular Medicine and Surgery, Karolinska Institutet, SE-171 76 Stockholm, Sweden. katarina.haapaniemi-kouru@karolinska.se
K
K
Haapaniemi Kouru
H
H
Malmgren
M
M
Nordenskjöld
M
M
Fridström
G
G
Csemiczky
E
E
Blennow
D000328:Adult; D001706:Biopsy / Q000379:methods*; D002869:Chromosome Aberrations; D041321:Chromosomes, Human, X / Q000235:genetics; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005306:Fertilization; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D010062:Ovulation Induction; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis / Q000379:methods*; D015203:Reproducibility of Results; D012189:Retrospective Studies; D013548:Sweden; D016896:Treatment Outcome