Direct biomolecule binding on nonfouling surfaces via newly discovered supramolecular self-assembly of lysozyme under physiological conditions.
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue" that binds strongly and quickly onto non-fouling coatings. This binding is highly selective towards lysozyme, and excludes synthetic, chemical/physical activation/deactivation (blocking) steps. By using biotinylated lysozyme, such a phase transition quickly creates a perfect biotinylated surface on non-fouling surfaces for avidin binding, showing great potential for the development of low-cost and practical biochips.
Center for Biologically Inspired Materials and Materials Systems-CBIMMS, Department of Biomedical Engineering, Duke University, NC 27705, USA. yangpengbuct@126.com
Peng
P
Yang
D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D002021:Buffers; D006531:HEPES; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry*; D044367:Phase Transition; D040081:Protein Array Analysis / Q000379:methods*; D012996:Solutions; D013499:Surface Properties
D002021:Buffers; D055767:Immobilized Proteins; D012996:Solutions; D001360:Avidin; D001710:Biotin; D009113:Muramidase; D006531:HEPES