The importance of being modified: PCNA modification and DNA damage response.
PCNA (proliferating cell nuclear antigen) is a sliding clamp that plays important roles during DNA replication and repair. In yeast, PCNA can be modified by either mono- or poly-ubiquitin or by addition of SUMO moieties. These different modifications direct the activity of PCNA toward alternative DNA transactions. In mammals, PCNA ubiquitination was reported, and it seems to have similar effects to those observed in yeast. However, for a long time, no SUMOylation of PCNA could be detected. Two recent papers report the detection of SUMOylated PCNA in mammalian cells. Here, we summarize similarities and differences between the various biological systems and present the current view of the way by which PCNA modification can affect DNA replication and repair pathways.
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel.
Inbal
I
Gazy
Martin
M
Kupiec
D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D004265:DNA Helicases / Q000378:metabolism; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D051135:Rad51 Recombinase / Q000378:metabolism; D025842:SUMO-1 Protein / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
C546328:ATAD5 protein, human; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C477609:Elg1 protein, S cerevisiae; D018809:Proliferating Cell Nuclear Antigen; D025842:SUMO-1 Protein; D029701:Saccharomyces cerevisiae Proteins; C061376:SRS2 protein, S cerevisiae; D051135:Rad51 Recombinase; D000251:Adenosine Triphosphatases; D000074183:ATPases Associated with Diverse Cellular Activities; D004265:DNA Helicases