Effects of mechanical strain on human mesenchymal stem cells and ligament fibroblasts in a textured poly(L-lactide) scaffold for ligament tissue engineering.
The purpose of this study was to prove the effect of cyclic uniaxial intermittent strain on the mRNA expression of ligament-specific marker genes in human mesenchymal stem cells (MSC) and anterior cruciate ligament-derived fibroblasts (ACL-fibroblasts) seeded onto a novel textured poly(L-lactide) scaffold (PLA scaffold). Cell-seeded scaffolds were mechanically stimulated by cyclic uniaxial stretching. The expression of ligament matrix gene markers: collagen types I and III, fibronectin, tenascin C and decorin, as well as the proteolytic enzymes matrix metalloproteinase MMP-1 and MMP-2 and their tissue specific inhibitors TIMP-1 and TIMP-2 was investigated by analysing the mRNA expression using reverse transcriptase polymerase chain reaction and related to the static control. In ACL-fibroblasts seeded on PLA, mechanical load induced up-regulation of collagen types I and III, fibronectin and tenascin C. No effect of mechanical stimulation on the expression of ligament marker genes was found in undifferentiated MSC seeded on PLA. The results indicated that the new textured PLA scaffold could transfer the mechanical load to the ACL-fibroblasts and improved their ligament phenotype. This scaffold might be suitable as a cell-carrying component of ACL prostheses.
Institute of Orthopaedic Research and Biomechanics, Center of Musculoskeletal Research, University of Ulm, Helmholtzstrasse 14, 89081 Ulm, Germany. ludwika.kreja@uni-ulm.de
Ludwika
L
Kreja
Astrid
A
Liedert
Heiter
H
Schlenker
Rolf E
RE
Brenner
Jörg
J
Fiedler
Benedikt
B
Friemert
Lutz
L
Dürselen
Anita
A
Ignatius
D001483:Base Sequence; D017931:DNA Primers; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008022:Ligaments / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological; D023822:Tissue Engineering; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism; D054457:Tissue Scaffolds
D017931:DNA Primers; D016326:Extracellular Matrix Proteins; D011091:Polyesters; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; C033616:poly(lactide); D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1