Quantitative and qualitative analysis of coagulation factors in cryoprecipitate prepared from fresh-frozen plasma inactivated with amotosalen and ultraviolet A light.
BACKGROUND
There were no previous studies about the quality of cryoprecipitate prepared from fresh-frozen plasma (FFP) inactivated with amotosalen and ultraviolet A (UVA) light. The aim of this study was to analyze the quantity and quality of coagulation factors in cryoprecipitate prepared from FFP treated with amotosalen and UVA light.


METHODS
FFP was obtained from whole blood donations and inactivated with amotosalen and UVA light according to the manufacturer's instructions. Fibrinogen, factor VIII (FVIII), von Willebrand factor antigen (VWF:Ag) and activity (VWF:RCo), the von Willebrand factor cleavage protease activity (ADAMTS-13), and the multimeric structure of VWF were analyzed.


RESULTS
The content of fibrinogen, FVIII, and ADAMTS-13 was lower in cryoprecipitates prepared from amotosalen-treated plasma when compared with cryoprecipitates prepared from nontreated plasma (35, 40, and 18% loss, respectively). The quantity and quality of VWF as well as VWF multimer patterns were not affected by the inactivation method.


CONCLUSIONS
Cryoprecipitates prepared from amotosalen-treated FFP contained significantly reduced levels of fibrinogen, FVIII, and ADAMTS-13. However, the VWF quantity and quality was well preserved.
Hemotherapy-Hemostasis Department, CDB, IDIBAPS, Hospital Clínic, University of Barcelona, Barcelona, Spain. jcid@clinic.ub.es
Joan
J
Cid
Carolina
C
Caballo
Marc
M
Pino
Ana M
AM
Galan
Nuria
N
Martínez
Ginés
G
Escolar
Maribel
M
Diaz-Ricart
D051722:ADAM Proteins / Q000032:analysis; D000071120:ADAMTS13 Protein; D001779:Blood Coagulation Factors / Q000032:analysis*; D058731:Blood Safety / Q000379:methods; D016022:Case-Control Studies; D005169:Factor VIII / Q000032:analysis / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry*; D011564:Furocoumarins / Q000494:pharmacology*; D006801:Humans; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D017319:Photosensitizing Agents / Q000494:pharmacology; D010865:Pilot Projects; D010949:Plasma / Q000737:chemistry*; D014466:Ultraviolet Rays; D014841:von Willebrand Factor / Q000032:analysis
D001779:Blood Coagulation Factors; D011564:Furocoumarins; D017319:Photosensitizing Agents; C026912:cryoprecipitate coagulum; D014841:von Willebrand Factor; D005169:Factor VIII; D005340:Fibrinogen; D051722:ADAM Proteins; D000071120:ADAMTS13 Protein; C099604:ADAMTS13 protein, human; C118577:amotosalen