The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to equine herpesvirus type 1 infection.
Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.
Division of Virology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Ciszewskiego 8, 02-786 Warsaw, Poland.
A
A
Golke
J
J
Cymerys
A
A
Słońska
T
T
Dzieciatkowski
A
A
Chmielewska
A
A
Tucholska
M W
MW
Bańbura
D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000662:veterinary; D002478:Cells, Cultured; D017097:Electric Impedance; D055724:Electrophysiological Phenomena; D004861:Herpesvirus 1, Equid / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000502:physiology / Q000821:virology*; D012867:Skin / Q000166:cytology*