[The use of hydrosol hexacianferrate (II) ferrum (III) for developing diagnostic lateral flow tests].
On the basis of synthesized negatively charged hydrosol hexacianferrate (II) ferrum (III) (HCFF) by diameter 10-20 HM are received stable conjugates with antibodies and antigens glycoprotein and lipopolysaccharide (LPS) nature. Synthesized hydrosol (HCFF) is new type of a disperse phase in the lateral flow assay. Conjugates mentioned above were applied for construction lateral flow tests-systems for revealing cholera toxin, the rabbit antibodies to recombinant glycoproteine complex from Micobacterium tuberculosis H37 Rv, human immunoglobulin, LPS antigens S. typhimurium, S. enteritidis. Developed lateral flow tests-systems had high analysis speed (5-7 min), good specificity and sensitivity: on cholera toxin of 2.0 ug/ml, on LPS antigens S. typhimurium, S. enteritidis 0.5 ug/ml.
S N
SN
Skopinskaia
S P
SP
Iarkov
V N
VN
Zlobin
Kh Kh
KhKh
Valiev
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D002772:Cholera Toxin / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry*; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011817:Rabbits; D012477:Salmonella enteritidis / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D005290:Ferric Compounds; D006023:Glycoproteins; D008070:Lipopolysaccharides; D002772:Cholera Toxin