Protective efficacy of crude virus-like particle vaccine against HPAI H5N1 in chickens and its application on DIVA strategy.
BACKGROUND
Currently, Asian lineage highly pathogenic avian influenza (HPAI) H5N1 has become widespread across continents. These viruses are persistently circulating among poultry populations in endemic regions, causing huge economic losses, and raising concerns about an H5N1 pandemic. To control HPAI H5N1, effective vaccines for poultry are urgently needed.


OBJECTIVE
In this study, we developed HPAI virus-like particle (VLP) vaccine as a candidate poultry vaccine and evaluated its protective efficacy and possible application for differentiating infected from vaccinated animals (DIVA).


METHODS
Specific pathogen-free chickens received a single injection of HPAI H5N1 VLP vaccine generated using baculovirus expression vector system. Immunogenicity of VLP vaccines was determined using hemagglutination inhibition (HI), neuraminidase inhibition (NI), and ELISA test. Challenge study was performed to evaluate efficacy of VLP vaccines.


CONCLUSIONS
A single immunization with HPAI H5N1 VLP vaccine induced high levels of HI and NI antibodies and protected chickens from a lethal challenge of wild-type HPAI H5N1 virus. Viral excretion from the vaccinated and challenged group was strongly reduced compared with a mock-vaccinated control group. Furthermore, we were able to differentiate VLP-vaccinated chickens from vaccinated and then infected chickens with a commercial ELISA test kit, which offers a promising strategy for the application of DIVA concept.
Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.
Jae-Keun
JK
Park
Dong-Hun
DH
Lee
Ha-Na
HN
Youn
Myeong-Seob
MS
Kim
Yu-Na
YN
Lee
Seong-Su
SS
Yuk
Tae-Hyun
TH
Lim
Jun-Hyuk
JH
Jang
Jung-Hoon
JH
Kwon
Byoung-Yoon
BY
Kim
Sang-Moo
SM
Kang
Baik-Lin
BL
Seong
Joong-Bok
JB
Lee
Seung-Yong
SY
Park
In-Soo
IS
Choi
Chang-Seon
CS
Song
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002645:Chickens; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology* / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D007252:Influenza Vaccines; D058425:Vaccines, Virus-Like Particle