Embedding matrix for simultaneous processing of multiple histological samples.
A method is described for simultaneous histological processing of multiple fixed tissue samples. The tissue samples are embedded in a gelatin-albumin protein matrix that is rapidly solidified and bound to the tissue surface using a cross-linking reagent. After freezing and sectioning, the individual sections containing multiple samples can be processed for immunocytochemical and histochemical staining. The method is demonstrated for simultaneous processing of multiple rodent brains, and for reconstruction of fragmented human postmortem brain samples.
Nathan Kline Institute for Psychiatric Research, Orangeburg, NY, USA. smiley@nki.rfmh.org
John F
JF
Smiley
Cynthia
C
Bleiwas
D000418:Albumins; D000818:Animals; D001921:Brain / Q000033:anatomy & histology; D005780:Gelatin; D006801:Humans; D007150:Immunohistochemistry; D016610:Tissue Embedding / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods
D000418:Albumins; D005780:Gelatin