Protective effect of ascorbic acid after single and repetitive administration of cadmium in Swiss mice.
The aim of the study was to delineate the protective effect of ascorbic acid with plausible mechanism after single and repetitive cadmium administration to Swiss mice. The effects of single high dose administration of CdCl(2) (6 mg/kg) or ascorbic acid (AsA) (50 mg/kg) and chronic (three times) administration of Cd at low dose (2 mg/kg) or AsA at same dose (50 mg/kg) were compared in Swiss albino mice. Changes of lipid peroxidation [determined by the malonyldialdehyde (MDA) concentration] were taken as a measure of the oxidative stress intensity. Lipid fatty acid's unsaturation related to the permeability of cell membranes was also examined. Mobilization of the immune system was determined by analyzing changes in antioxidant concentrations of AsA and glutathione (GSH), and by measuring the activation of antioxidant enzymes SOD, GPx and CAT. In addition, the level of free polyamines and variation in their proportions were examined. In conclusion, exposure to higher levels of cadmium will have more deleterious effects on the body rather than chronic exposure at lower levels with this toxic metal, while this study clearly demonstrated the protective effects of AsA in a mouse model.
Department of Animal Physiology and Toxicology, Institute of Biology, Pedagogical University of Cracow, Cracow, Poland. agrenagren@gmail.com
Agnieszka
A
Greń
Anna
A
Barbasz
Barbara
B
Kreczmer
Apolonia
A
Sieprawska
Elżbieta
E
Rudolphi-Skórska
Maria
M
Filek
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D001923:Brain Chemistry; D019256:Cadmium Chloride / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004785:Environmental Pollutants / Q000633:toxicity*; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D007107:Immune System / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D013997:Time Factors
D000975:Antioxidants; D004785:Environmental Pollutants; D005227:Fatty Acids; D008315:Malondialdehyde; D010088:Oxidoreductases; D019256:Cadmium Chloride; D001205:Ascorbic Acid