[Experimental study of the effect of adipose stromal vascular fraction cells with VEGF on the neovascularization of free fat transplantation].
OBJECTIVE
To investigate the effect of adipose stromal vascular fraction cells (SVFs) with VEGF on the neovascularization of free fat transplantation.


METHODS
SVFs were obtained from subcutaneous fat and labelled with DiI. 0.3 ml autologous fat tissue was mixed with 0.2 ml cells: 1) autologous SVFs with VEGF (Group A); 2) autologous SVFs (Group B); 3) complete DMEM (Group C) And then the mixture was injected randomly under the back skin of 12 nude mice. The transplanted fat tissue in three groups was harvested at 2 months after implantation. Wet weight and diameter of fat grafts was measured. After HE and CD31 staining,blood vessel density, viable adipocytes and fibrous proliferation were observed.


RESULTS
Trace of SVFs labeled by DiI in vivo could be detected by fluorescent microscope. The wet weight of fat grafts was (191.90 +/- 9.81) mg in group A, (177.01 +/- 10.50) mg in group B, and (92.05 +/- 8.30) mg in group C (P<0.01). The diameter of fat grafts was (0.49 +/- 0.24) cm in group A, (0.40 +/- 0.26) cm in group B, and (0.32 +/- 0.28) cm in group C (P<0.01). Histological analysis showed the blood vessel density was (14.58 +/- 2.06)/HPL in group A, (11.55 +/- 2.18)/HPL in group B, (7.87 +/- 1.55)/HPL in group C. Compared with group B and group C, group A had more adipose tissue with less fat necrosis and fibrosis and had significantly higher capillary density.


CONCLUSIONS
The autologous adipose stromal vascular fraction cells with VEGF could improve the neovascularization of free fat significantly. It indicates a wide clinical application in the future.
Department of Plastic Surgery, Nanfang Hospital of Southern Medical University, Guangzhou 510515, China.
Li-Qun
LQ
Li
Jian-Hua
JH
Gao
Feng
F
Lu
Jun-Da
JD
Zheng
Qiu-Xiang
QX
He
Bin-Ting
BT
Ni
Sheng-Sheng
SS
Pan
D017667:Adipocytes; D000273:Adipose Tissue / Q000033:anatomy & histology / Q000098:blood supply* / Q000637:transplantation*; D000818:Animals; D002196:Capillaries; D006085:Graft Survival; D051379:Mice; D008819:Mice, Nude; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D009929:Organ Size; D017154:Stromal Cells / Q000637:transplantation*; D042461:Vascular Endothelial Growth Factor A / Q000627:therapeutic use*
D042461:Vascular Endothelial Growth Factor A