Cadmium-induced DNA damage and mutations in Arabidopsis plantlet shoots identified by DNA fingerprinting.
Random amplified polymorphic DNA (RAPD) test is a feasible method to evaluate the toxicity of environmental pollutants on vegetal organisms. Herein, Arabidopsis thaliana (Arabidopsis) plantlets following Cadmium (Cd) treatment for 26 d were screened for DNA genetic alterations by DNA fingerprinting. Four primers amplified 20-23 mutated RAPD fragments in 0.125-3.0 mg L(-1) Cd-treated Arabidopsis plantlets, respectively. Cloning and sequencing analysis of eight randomly selected mutated fragments revealed 99-100% homology with the genes of VARICOSE-Related, SLEEPY1 F-box, 40S ribosomal protein S3, phosphoglucomutase, and noncoding regions in Arabidopsis genome correspondingly. The results show the ability of RAPD analysis to detect significant genetic alterations in Cd-exposed seedlings. Although the exact functional importance of the other mutated bands is unknown, the presence of mutated loci in Cd-treated seedlings, prior to the onset of significant physiological effects, suggests that these altered loci are the early events in Cd-treated Arabidopsis seedlings and would greatly improve environmental risk assessment.
Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. liuwan63@hotmail.com
Wan
W
Liu
Lizong
L
Sun
Ming
M
Zhong
Qixing
Q
Zhou
Zongqiang
Z
Gong
Peijun
P
Li
Peidong
P
Tai
Xiaojun
X
Li
D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D002104:Cadmium / Q000633:toxicity*; D004249:DNA Damage; D016172:DNA Fingerprinting; D009154:Mutation; D036226:Seedlings / Q000187:drug effects / Q000502:physiology; D012989:Soil Pollutants / Q000633:toxicity*
D012989:Soil Pollutants; D002104:Cadmium