Rigidity guided cell attachment on inkjet-printed patterns.
A new approach is presented to control cell attachment behavior on biocompatible substrates. Multiple layers of polylactic acid (PLA) were inkjet-printed on dry alginate films to create composite surfaces with rigidity variation. The printed films were submerged in cell culture medium and fibroblast 3T3-L1 cells were cultured on the printed films. 3T3-L1 cells were found to preferentially adhere on PLA surfaces with higher rigidity. The same approach was also used to create various cell attachment patterns. This study provides a new methodology to fabricate biodegradable matrix for favorable cell adhesion or patterning.
Department of Chemical Engineering, ‡Department of Mechanical Engineering, National Taiwan University, Taipei, Taiwan.
Ying-Chih
YC
Liao
Yun-Ting
YT
Ma
Chi-Hui
CH
Huang
Jiashing
J
Yu
Hao-Ming
HM
Hsiao
D041721:3T3-L1 Cells; D000464:Alginates / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000295:instrumentation; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006244:Hardness / Q000502:physiology*; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
D000464:Alginates; D020099:Coated Materials, Biocompatible; D006603:Hexuronic Acids; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D020723:Glucuronic Acid