Salinity-induced changes in protein expression in the halophytic plant Nitraria sphaerocarpa.
Salinity is a major abiotic stress that inhibits plant growth and development. Plants have evolved complex adaptive mechanisms that respond to salinity stress. However, an understanding of how plants respond to salinity stress is far from being complete. In particular, how plants survive salinity stress via alterations to their intercellular metabolic networks and defense systems is largely unknown. To delineate the responses of Nitraria sphaerocarpa cell suspensions to salinity, changes in their protein expression patterns were characterized by a comparative proteomic approach. Cells that had been treated with 150 mM NaCl for 1, 3, 5, 7, or 9 days developed several stress-related phenotypes, including those affecting morphology and biochemical activities. Of ~1100 proteins detected in 2-DE gel patterns, 130 proteins showed differences in abundance with more than 1.5-fold when cells were stressed by salinity. All but one of these proteins was identified by MS and database searching. The 129 spots contained 111 different proteins, including those involved in signal transduction, cell rescue/defense, cytoskeleton and cell cycle, protein folding and assembly, which were the most significantly affected. Taken together, our results provide a foundation to understand the mechanism of salinity response.
Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
Jinhui
J
Chen
Tielong
T
Cheng
Pengkai
P
Wang
Weidong
W
Liu
Jiao
J
Xiao
Yunqiang
Y
Yang
Xiangyang
X
Hu
Zeping
Z
Jiang
Shougong
S
Zhang
Jisen
J
Shi
D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D046228:Microarray Analysis; D008954:Models, Biological; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D054712:Salinity; D055051:Salt-Tolerant Plants / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D053719:Tandem Mass Spectrometry; D028461:Zygophyllaceae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins; D020543:Proteome; D012965:Sodium Chloride