Tumor suppressor BLU inhibits proliferation of nasopharyngeal carcinoma cells by regulation of cell cycle, c-Jun N-terminal kinase and the cyclin D1 promoter.
BACKGROUND
Tumor suppressor genes function to regulate and block tumor cell proliferation. To explore the mechanisms underlying the tumor suppression of BLU/ZMYND10 gene on a frequently lost human chromosomal region, an adenoviral vector with BLU cDNA insert was constructed.


METHODS
BLU was re-expressed in nasopharyngeal carcinoma cells by transfection or viral infection. Clonogenic growth was assayed; cell cycle was analyzed by flow cytometry-based DNA content detection; c-Jun N-terminal kinase (JNK) and cyclin D1 promoter activities were measured by reporter gene assay, and phosphorylation was measured by immunoblotting. The data for each pair of groups were compared with Student t tests.


RESULTS
BLU inhibits clonogenic growth of nasopharyngeal carcinoma cells, arrests cell cycle at G1 phase, downregulates JNK and cyclin D1 promoter activities, and inhibits phosphorylation of c-Jun.


CONCLUSIONS
BLU inhibits growth of nasopharyngeal carcinoma cells by regulation of the JNK-cyclin D1 axis to exert tumor suppression.
Department of Pathophysiology, Guangdong Medical College, 1 Xincheng Road, Song-Shan Lake SSL Science Technology and Industrial Park Dongguan, Guangdong, 523808, China. Zhangxn_2006@126.com
Xiangning
X
Zhang
Hui
H
Liu
Binbin
B
Li
Peichun
P
Huang
Jianyong
J
Shao
Zhiwei
Z
He
D000256:Adenoviridae; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism*; D003598:Cytoskeletal Proteins; D015536:Down-Regulation; D016193:G1 Phase / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000502:physiology*; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000502:physiology*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000502:physiology*
D003598:Cytoskeletal Proteins; D025521:Tumor Suppressor Proteins; C472720:ZMYND10 protein, human; D019938:Cyclin D1; D048031:JNK Mitogen-Activated Protein Kinases