Detection and identification of Vibrio species using whole-cell protein pattern analysis.
Outbreaks of foodborne diseases associated with Vibrio species such as V. parahaemolyticus, V. vulnificus, and V. cholerae frequently occur in countries having a dietary habit of raw seafood consumption. For rapid identification of different Vibrio species involved in foodborne diseases, whole-cell protein pattern analysis for 13 type strains of 12 Vibrio species was performed using SDS-PAGE analysis. Pathogenic Vibrio species such as V. parahaemolyticus, V. vulnificus, V. cholerae, V. alginolyticus, V. fluvialis, and V. mimicus were included in the 12 Vibrio species used in this study. Each of the 12 Vibrio species showed clearly specific band patterns of its own. Two different strains of V. parahaemolyticus showed two different SDS-PAGE wholecell protein patterns, giving the possibility of categorizing isolated strains in the same V. parahaemolyticus species into two subgroups. The 36 Vibrio isolates collected from sushi restaurants in Busan were all identified as V. parahaemolyticus by comparing their protein patterns with those of Vibrio type strains. The identified isolates were categorized into two different subgroups of V. parahaemolyticus. The whole-cell protein pattern analysis by SDS-PAGE can be used as a specific, rapid, and simple identification method for Vibrio spp. involved in foodborne diseases at the subspecies level.
Department of Food Science & Technology, Ewha Woman's University, Seoul 120-750, Korea.
Chae-Yoon
CY
Lee
Yeun
Y
Hong
Jio
J
Ryu
Young-Rok
YR
Kim
Sang-Suk
SS
Oh
Soon-Ho
SH
Lee
In-Gyun
IG
Hwang
Hae-Yeong
HY
Kim
D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005516:Food Microbiology; D020543:Proteome / Q000032:analysis*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014733:Vibrio / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification
D001426:Bacterial Proteins; D020543:Proteome