Involvement of virulence properties and antibiotic resistance in Escherichia coli strains causing pyelonephritis in children.
A total of 145 Escherichia coli strains causing pyelonephritis in children were investigated for the prevalence of genes encoding the following virulence factors (VFs): P fimbria (67.6 %), S fimbria (53.8 %), AFA adhesins (2.8 %), cytotoxic necrotizing factor 1 (37.9 %), α-hemolysin (41.4 %), and aerobactin (71.7 %). One hundred and thirty-six (93.8 %) isolates harbored at least one of the virulence genes detected in the present study. Statistically significant co-occurrent presence of two VF genes was found for α-hly-cnf1, α-hly-sfa, cnf1-sfa (p<0.001), and α-hly-pap (p=0.001). Twenty-six profiles of VF genes were detected in this study. The combinations of aer-pap and aer-pap-sfa-α-hly-cnf1 were presented with the highest frequency-both of them in 28 isolates (19.3 %). All E. coli strains included in the study were susceptible to meropenem, amikacin, and tobramycin; the highest frequency resistance was found toward ampicillin (43.4 %), piperacillin (31.7 %), tetracycline (15.9 %), and trimethoprim/sulfamethoxazole (11.7 %). The resistance to the other tested antimicrobial drugs did not exceed 3 % incidence. Overall, 55.9 % strains were susceptible to all tested anti-infective agents. Antimicrobial resistance of E. coli strains toward trimethoprim/sulfamethoxazole statistically significantly correlated with the presence of α-hly (p<0.001), sfa (p<0.01), and cnf1 (p<0.05).
Institute of Microbiology, Faculty of Medicine, Comenius University and University Hospital Bratislava, Sasinkova 4, 811 08, Bratislava, Slovak Republic. jan.koren@fmed.uniba.sk
Ján
J
Koreň
Katarína
K
Čurová
Marta
M
Kmeťová
Leonard
L
Siegfried
Viktor
V
Jankó
László
L
Kovács
Helena
H
Hupková
Ján
J
Luha
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000472:pathogenicity*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D011704:Pyelonephritis / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*
D037521:Virulence Factors