Prediction of the stability of modified RNA duplexes based on deformability analysis: oligoribonucleotide derivatives modified with 2'-O-cyanoethyl-5-propynyl-2-thiouridine as a promising component.
We describe a method to predict the stability of a modified RNA duplex. Ten unique modified RNA duplexes showed a linear relationship between the calculated and experimentally determined duplex stabilities.
Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, Japan.
Yoshiaki
Y
Masaki
Ryuta
R
Miyasaka
Kunihiro
K
Hirai
Hirosuke
H
Tsunoda
Akihiro
A
Ohkubo
Kohji
K
Seio
Mitsuo
M
Sekine
D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D020871:RNA Stability; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000737:chemistry; D044366:Transition Temperature
C105273:2-thiouridine; D009843:Oligoribonucleotides; D013891:Thiouridine; D012313:RNA