Development of a serotyping enzyme-linked immunosorbent assay system based on recombinant truncated hantavirus nucleocapsid proteins for New World hantavirus infection.
New World hantaviruses were divided into five groups based on the amino acid sequence variability of the internal variable region (around 230-302 amino acids) of hantavirus nucleocapsid protein (NP). Sin Nombre virus (SNV), Andes virus, Black Creek Canal virus (BCCV), Carrizal virus (CARV) and Cano Delgadito virus belong to groups 1, 2, 3, 4 and 5, respectively. Patient and rodent sera were serotyped successfully by an enzyme-linked immunosorbent assay (ELISA) with recombinant truncated NP lacking 99 N-terminal amino acids (trNP100) of SNV, CARV and BCCV. The trNP100 of BCCV showed lower reactivity to heterologous sera. In contrast, whole recombinant NP antigens detected both homologous and heterologous antibodies equally. The results together with results of a previous study suggest that trNP100 can distinguish infections among viruses in groups 1, 2, 3 and 4 of New World hantaviruses. The serotyping ELISA with trNP100 is useful for epidemiological surveillance in humans and rodents.
Department of Microbiology, Graduate School of Medicine, Hokkaido University, Kita-ku, Kita-15, Nishi-7, Sapporo 060-8638, Japan.
Takaaki
T
Koma
Kumiko
K
Yoshimatsu
Midori
M
Taruishi
Daisuke
D
Miyashita
Rika
R
Endo
Kenta
K
Shimizu
Shumpei P
SP
Yasuda
Takako
T
Amada
Takahiro
T
Seto
Ryo
R
Murata
Haruka
H
Yoshida
Hiroaki
H
Kariwa
Ikuo
I
Takashima
Jiro
J
Arikawa
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006476:Orthohantavirus / Q000145:classification* / Q000302:isolation & purification; D018778:Hantavirus Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D012377:Rodentia; D017422:Sequence Analysis, DNA; D012703:Serotyping / Q000379:methods; D014773:Virology / Q000379:methods*
D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins