Inhibition of S100A9 expression by propofol in monocytes of rats with             endotoxemia.
The inflammatory response is a non-specific autoimmune response. Monocytes             are the most important effector cells in the systemic inflammatory response. In             recent years, the function of the intravenous anesthetic, propofol, in the inhibition             of the inflammatory response has attracted much attention. However, the specific             signal transduction mechanism related to the anti-inflammatory effect of propofol             remains unclear. In this study, monocyte protein expression in rats with endotoxemia             was detected using proteomic techniques before and after propofol intervention.             By two-dimensional electrophoresis and mass spectrometric identification, we found             that S100A9 protein expression was significantly reduced after propofol treatment.             In addition, we used western blot analysis to confirm the results of two-dimensional             electrophoresis. The S100A9 protein, a member of the S100A calcium-binding protein             family, is closely related to the occurrence and development of inflammation.             The results of this study suggest that the anti-inflammatory effect of propofol             may be related to the inhibition of S100A9 protein expression.
Department of Anesthesiology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, PR China.
Zhen
Z
Li
Guodong
G
Zhao
Guobin
G
Zhou
Yan
Y
Wang
Qing
Q
Wang
Jinquan
J
Ji
Zhipeng
Z
Wang
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D040502:Calgranulin B / Q000737:chemistry / Q000378:metabolism*; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D019446:Endotoxemia / Q000378:metabolism* / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D015742:Propofol / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000893:Anti-Inflammatory Agents; D040502:Calgranulin B; D008070:Lipopolysaccharides; D020543:Proteome; D015742:Propofol