AFB(1) -induced mutagenesis of the gpt gene in AS52 cells.
Aflatoxin B(1) (AFB(1) ) is a potent mutagen and an important risk factor for hepatocellular carcinoma (HCC) in humans. Transgenic mouse strains and cells in culture have been used to detect different types of mutations caused by AFB(1) and investigate the molecular determinants of their location and frequency. The AFB(1) mutational spectrum in the gpt gene was markedly different in AS52 cells compared with the liver in gpt delta B6C3F1 transgenic mice. The results demonstrate the importance of metabolism, chromosomal location, transcription and selection conditions on mutational spectra.
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Roongtiwa
R
Wattanawaraporn
Min Young
MY
Kim
Jillian
J
Adams
Laura J
LJ
Trudel
Leslie L
LL
Woo
Robert G
RG
Croy
John M
JM
Essigmann
Gerald N
GN
Wogan
D016604:Aflatoxin B1 / Q000633:toxicity*; D000410:Alanine Transaminase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009154:Mutation / Q000187:drug effects*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D016604:Aflatoxin B1; D000410:Alanine Transaminase