Role of autologous mesenchymal stem cells associated with platelet-rich plasma on healing of cutaneous wounds in diabetic mice.
BACKGROUND
Chronic cutaneous lesions affect 15% of human patients with diabetes, and the associated risk of limb amputations is 15-46 times greater than that of people with normal glycaemia. It is estimated that half of these limb amputations could be avoided by opportune treatment with somatic stem cells or platelet-rich plasma (PRP).


METHODS
We evaluated the effects of autologous transplant of mesenchymal stem cells (MSCs) with or without combination with autologous PRP in the re-epithelialization of cutaneous lesions induced in diabetic mice.


RESULTS
Animals treated with MSCs alone showed a similar level of re-epithelialization of cutaneous lesions to those treated with MSC plus PRP, and no significant difference was found between the two treatments.


CONCLUSIONS
Both treatments gave better results than daily cleaning of the cutaneous lesions with saline or covering of the lesions with semipermeable adherent bandage.
Department of Veterinary Medicine, Federal University of Piauí, Teresina, Brazil. argolo_napoleao@hotmail.com
N M
NM
Argôlo Neto
R J
RJ
Del Carlo
B S
BS
Monteiro
N B
NB
Nardi
P C
PC
Chagastelles
A F S
AF
de Brito
A M S
AM
Reis
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D003094:Collagen / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000150:complications*; D003922:Diabetes Mellitus, Type 1 / Q000139:chemically induced / Q000150:complications*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D053657:Platelet-Rich Plasma; D012867:Skin / Q000293:injuries*; D014182:Transplantation, Autologous; D014945:Wound Healing / Q000502:physiology*
D015703:Antigens, CD; D003094:Collagen