ICOS, SLAM and PD-1 expression and regulation on T lymphocytes reflect the immune dysregulation in patients with HIV-related illness with pulmonary tuberculosis.
BACKGROUND
Tuberculosis (TB) continues to be the most frequent cause of illness and death from an infectious agent globally, and its interaction with HIV is having devastating effects. To investigate how HIV alters the immune response to Mycobacterium tuberculosis (Mtb), we assessed basal and Mtb-induced proliferation, cytokine production, and expression of signalling lymphocytic activation molecule (SLAM), inducible costimulator (ICOS) and programmed death-1 (PD-1) on T lymphocytes from HIV-positive individuals coinfected with TB, HIV-positive subjects, TB patients and healthy donors (HD).


RESULTS
HIV-TB patients showed increased ICOS, SLAM and PD-1 basal levels on T lymphocytes, whereas HIV-positive individuals displayed elevated levels of SLAM and PD-1, TB patients high levels of SLAM, and HD low levels of the three proteins. Mtb-stimulation enhanced ICOS expression in the four groups, but only TB and HD increased SLAM and PD-1 levels.


CONCLUSIONS
These data show the immune deregulation that takes place during the immune response against TB in different study populations.
Department of Biological Chemistry, University of Buenos Aires, School of Sciences, Buenos Aires, Argentina.
Javier Oscar
JO
Jurado
Virginia
V
Pasquinelli
Ivana Belén
IB
Alvarez
Gustavo Javier
GJ
Martínez
Natalia
N
Laufer
Omar
O
Sued
Pedro
P
Cahn
Rosa María
RM
Musella
Eduardo
E
Abbate
Horacio
H
Salomón
María Florencia
MF
Quiroga
D015703:Antigens, CD / Q000096:biosynthesis*; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D020869:Gene Expression Profiling; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D060889:Inducible T-Cell Co-Stimulator Protein / Q000096:biosynthesis*; D061026:Programmed Cell Death 1 Receptor / Q000096:biosynthesis*; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
D015703:Antigens, CD; D016207:Cytokines; C556692:ICOS protein, human; D060889:Inducible T-Cell Co-Stimulator Protein; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; D011956:Receptors, Cell Surface; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1