Activity-dependent A-to-I RNA editing in rat cortical neurons.
Changes in neural activity influence synaptic plasticity/scaling, gene expression, and epigenetic modifications. We present the first evidence that short-term and persistent changes in neural activity can alter adenosine-to-inosine (A-to-I) RNA editing, a post-transcriptional site-specific modification found in several neuron-specific transcripts. In rat cortical neuron cultures, activity-dependent changes in A-to-I RNA editing in coding exons are present after 6 hr of high potassium depolarization but not after 1 hr and require calcium entry into neurons. When treatments are extended from hours to days, we observe a negative feedback phenomenon: Chronic depolarization increases editing at many sites and chronic silencing decreases editing. We present several different modulations of neural activity that change the expression of different mRNA isoforms through editing.
Broad Institute, Cambridge, Massachusetts 02142, USA. neville@broadinstitute.org
Neville E
NE
Sanjana
Erez Y
EY
Levanon
Emily A
EA
Hueske
Jessica M
JM
Ambrose
Jin Billy
JB
Li
D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology*; D007288:Inosine / Q000378:metabolism*; D008564:Membrane Potentials; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D017393:RNA Editing; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013569:Synapses / Q000378:metabolism; D013997:Time Factors
D012333:RNA, Messenger; D007288:Inosine; D000241:Adenosine; D002118:Calcium