Characterization of grapevine microR164 and its target genes.
MicroRNAs (miRNAs) are an extensive class of newly identified small RNAs that regulate gene expression at post-transcription level by mRNA cleavage or translation. In our study, we used qRT-PCR and found that Vv-miR164 is expression in grapevine leaves, stems, tendrils, inflorescences, flowers and fruits. In addition, two potential target genes for Vv-miR164 were also found and verified by PPM-RACE and RLM-RACE. The results not only maps the cleavage site of the target mRNA but allowed for detection the expression pattern of cleaved fragments that can indicate the regulatory function of this miRNA on its target genes. These target genes were explored by qRT-PCR where some exhibited different expression patterns from their corresponding miRNA, indicating the cleavage mode of the miRNA on its target genes. The efficient and powerful approach used in this study can help in further understanding of how miRNAs cleaved their target mRNAs. Results from this study prove the importance of Vv-miR164 in regulating development and growth of grapes, and adds to the existing knowledge of small RNA-mediated regulation in grapes.
College of Horticulture, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095 Jiangsu Province, People's Republic of China. 2010104017@njau.edu.cn
Xin
X
Sun
Nicholas Kibet
NK
Korir
Jian
J
Han
Ling-Fei
LF
Shangguan
Emrul
E
Kayesh
Xiang-Peng
XP
Leng
Jing-Gui
JG
Fang
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D035261:Plant Components, Aerial / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D027843:Vitis / Q000235:genetics* / Q000378:metabolism
D035683:MicroRNAs; D010940:Plant Proteins; D018749:RNA, Plant