Utility of immunohistochemical analysis of KAI1, epithelial-specific antigen, and epithelial-related antigen for distinction of chromophobe renal cell carcinoma, an eosinophilic variant from renal oncocytoma.
Distinction of renal oncocytoma (RO) from chromophobe renal cell carcinoma (ChRCC) is important because their clinical behavior is different. As part of a search for the best available immunohistochemical markers to distinguish ChRCC from RO, we investigated the immunohistochemical profiles of these tumors. We selected 30 renal tumors consisting of ChRCC, typical variant (n = 14), ChRCC, eosinophilic variant (n = 6), and RO (n = 10). Their expression of cytokeratin (CK) 7, KAI1, epithelial-specific antigen (ESA), epithelial-related antigen (ERA), Claudin- 7, and Claudin-8 was studied using an autostainer. Immunoreactivity was assessed based on a combined score of the extent and intensity of staining. Compared to RO, a significantly higher percentage of the total ChRCCs stained positive for CK7 (85% vs. 10%, respectively), KAI1 (90% vs. 10%), ESA (95% vs. 10%), ERA (95% vs. 10%), and Claudin-7 (95% vs. 20%) (P < 0.001). Additionally, there was a significant difference between the percentage of ChRCC eosinophilic variant (ChRCC-E) and RO that stained positive for KAI1 (100% vs. 10%, respectively), ESA (83% vs. 10%), and ERA (83% vs. 10%) (P < 0.001). We recommend immunohistochemical analysis of KAI1, ESA, and ERA to distinguish ChRCC-E from RO.
Department of Pathology, Kansai Medical University, Hirakata Hospital, 2-3-1 Shinmachi, Hirakata, Osaka, 573-1191, Japan.
Chisato
C
Ohe
Naoto
N
Kuroda
Kosho
K
Takasu
Hideto
H
Senzaki
Nobuaki
N
Shikata
Tadanori
T
Yamaguchi
Chika
C
Miyasaka
Yorika
Y
Nakano
Noriko
N
Sakaida
Yoshiko
Y
Uemura
D018249:Adenoma, Oxyphilic / Q000175:diagnosis / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000175:diagnosis / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D057167:Claudins / Q000378:metabolism; D003937:Diagnosis, Differential; D004801:Eosine Yellowish-(YS) / Q000378:metabolism; D000071858:Epithelial Cell Adhesion Molecule; D006801:Humans; D007150:Immunohistochemistry; D051861:Kangai-1 Protein / Q000378:metabolism*; D053552:Keratin-7 / Q000378:metabolism; D007680:Kidney Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D012680:Sensitivity and Specificity
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C497688:CD82 protein, human; C473255:CLDN7 protein, human; D015815:Cell Adhesion Molecules; D057167:Claudins; D000071858:Epithelial Cell Adhesion Molecule; D051861:Kangai-1 Protein; D053552:Keratin-7; C502011:claudin 8; D004801:Eosine Yellowish-(YS)