Damage repair effect of He-Ne laser on wheat exposed to enhanced ultraviolet-B radiation.
We explored the use of He-Ne laser on alleviating the effects of ultraviolet-B (UV-B) light on winter wheat development. Triticum aestivum L. cv. Linyuan 077038 seeds were irradiated with either UV-B (10.08 kJ m(-2) d(-1)) (enhanced UV-B) or a combination of UV-B light and the He-Ne laser (5.43 mW mm(-2)). Plants also were exposed to the He-Ne laser alone. Our results showed that enhanced UV-B produced negative effects on seed germination and seedling development. Germination rate and shoot growth decreased compared with the control. Root development was inhibited, and root length was decreased. Chlorophyll content and expression of peroxidase (POD) isozymes and their activity decreased. Seedling height and shoot biomass dropped significantly compared to the control. Implementing the He-Ne laser partially alleviated the injury of enhanced UV-B radiation, because germination rate and shoot growth were enhanced together with root development. Chlorophyll content and POD expression and activity increased. Seedling height and shoot biomass were increased. Furthermore, the use of the He-Ne laser alone showed a favorable effect on seedling growth compared with the control.
Shanxi Normal University, Linfen 041004, PR China.
Liyan
L
Yang
Rong
R
Han
Yi
Y
Sun
D018525:Germination / Q000528:radiation effects; D007834:Lasers; D036226:Seedlings / Q000254:growth & development / Q000528:radiation effects*; D014908:Triticum / Q000254:growth & development / Q000528:radiation effects*; D014466:Ultraviolet Rays