Sinomicrobium oceani gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment.
A marine bacterium, designated SCSIO 03483(T), was isolated from a marine sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or marine agar 2216. Optimal growth occurred in the presence of 0-4 % (w/v) NaCl, at pH 7.0 and a temperature range of 28-37 °C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1(T) (92.7 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The DNA G+C content of strain SCSIO 03483(T) was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483(T) ( = KCTC 23994(T) = CGMCC 1.12145(T)).
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.|Key Laboratory of Marine Bio-resources Sustainable Utilization (CAS), RNAM Center for Marine Microbiology (CAS), Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
Ying
Y
Xu
Key Laboratory of Marine Bio-resources Sustainable Utilization (CAS), RNAM Center for Marine Microbiology (CAS), Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
Xin-Peng
XP
Tian
Key Laboratory of Marine Bio-resources Sustainable Utilization (CAS), RNAM Center for Marine Microbiology (CAS), Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
Yu-Juan
YJ
Liu
Key Laboratory of Marine Bio-resources Sustainable Utilization (CAS), RNAM Center for Marine Microbiology (CAS), Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
Jie
J
Li
Korea Research Institutes of Biosciences and Biotechnology, 52 Eoeun-dong, Yuseong gu, Daejeon 305-333, Republic of Korea.
Chang-Jin
CJ
Kim
Key Laboratory of Marine Bio-resources Sustainable Utilization (CAS), RNAM Center for Marine Microbiology (CAS), Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
Hao
H
Yin
Key Laboratory of Biogeography and Bioresource in Arid Land, Chinese Academy of Science, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Wen-Jun
WJ
Li
Key Laboratory of Marine Bio-resources Sustainable Utilization (CAS), RNAM Center for Marine Microbiology (CAS), Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
Si
S
Zhang
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6