Theoretical and in vitro studies of a C-terminal peptide from PGKC of Leishmania mexicana mexicana.
Trypanosomatids cause deadly diseases in humans. Of the various biochemical pathways in trypanosomatids, glycolysis, has received special attention because of being sequestered in peroxisome like organelles critical for the survival of the parasites. This study focuses on phosphoglycerate kinase (PGK) from Leishmania spp. which, exists in two isoforms, the cytoplasmic PGKB and glycosomal PGKC differing in their biochemical properties. Computational analysis predicted the likelihood of a transmembrane helix only in the glycosomal isoform PGKC, of approximate length 20 residues in the 62-residue extension, ending at, arginine residues R471 and R472. From experimental studies using circular dichroism and NMR with deuterated sodium dodecyl sulfate, we find that the transmembrane helix spans residues 448±2 to 476 in Leishmania mexicana PGKC. The significance of this observation is discussed in the context of glycosomal transport and substrate tunneling.
National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India.
Sandeep
S
Kaushik
Bankala
B
Krishnarjuna
Srinivasarao
S
Raghothama
Sangita
S
Aggarwal
Vidya
V
Raghunathan
Anjali
A
Ganjiwale
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D019295:Computational Biology; D003593:Cytoplasm / Q000201:enzymology / Q000235:genetics; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D007894:Leishmania mexicana / Q000201:enzymology* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins / Q000737:chemistry; D008823:Micelles; D008830:Microbodies / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D010735:Phosphoglycerate Kinase / Q000737:chemistry*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D015800:Protozoan Proteins / Q000737:chemistry*; D012995:Solubility; D013379:Substrate Specificity
D007527:Isoenzymes; D008565:Membrane Proteins; D008823:Micelles; D010455:Peptides; D015800:Protozoan Proteins; D010735:Phosphoglycerate Kinase