Inhibition of human gastric carcinoma cell growth in vitro by a polysaccharide from Aster tataricus.
A water-soluble polysaccharide (WATP), with a molecular weight of 6.3 × 10⁴ Da, was isolated from Aster tataricus. According to gas chromatography (GC) analysis, WATP was composed of galactose, glucose, fucose, rhamnose, arabinose and mannose with molar ratios of 2.1:1.3:0.9:0.5:0.3:0.6. The effects of WATP on cell proliferation and apoptosis in human gastric cancer SGC-7901 cells were examined. MTT assay showed that WATP had a perfectly tumor growth inhibitory activity on SGC-7901 cells, but no cytotoxicity on SGC-7901 and primary human polymorphonuclear (PMN) cells analyzed using LDH assay. Flow cytometry analysis indicated that WATP could significantly induce apoptosis of SGC-7901 cells. Furthermore using Rh123 and Fluo-3 as fluorescent probes, respectively, it was found that mitochondrial transmembrane potential (ΔΨ(m)) of treatment groups was significantly lower than that in un-treatment group and the concentration of calcium in cells exposed to WATP for 24 h was increased in a dose dependent manner compared with unexposed group. These results suggest that WATP induces apoptosis of SGC-7901 cells through calcium- and ΔΨ(m)-dependent pathways, indicating that it is potentially useful as a natural anti-cancer agent.
Department of General Surgery, People's Hospital, Peking University, Beijing 100044, PR China.
Yunxin
Y
Zhang
Qiusheng
Q
Wang
Tie
T
Wang
Haikui
H
Zhang
Ying
Y
Tian
Hong
H
Luo
Shen
S
Yang
Yuan
Y
Wang
Xun
X
Huang
D017209:Apoptosis / Q000187:drug effects; D031173:Aster Plant / Q000737:chemistry*; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D012995:Solubility; D013274:Stomach Neoplasms / Q000473:pathology*; D014867:Water / Q000737:chemistry
D011134:Polysaccharides; D014867:Water; D002118:Calcium