Amplified fluorescence polarization aptasensors based on structure-switching-triggered nanoparticles enhancement for bioassays.
We have developed an amplified fluorescence polarization aptasensor that relies on aptamer structure-switching-triggered nanoparticles (NPs) enhancement for biomolecules detection. This new type of assay exhibits higher detection sensitivity over traditional homogeneous aptasensors by two orders of magnitude and high specificity for target molecules.
Ministry of Education Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China.
Yong
Y
Huang
Shulin
S
Zhao
Zhen-Feng
ZF
Chen
Ming
M
Shi
Hong
H
Liang
D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D005454:Fluorescence Polarization / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013917:Thrombin / Q000032:analysis*
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D000255:Adenosine Triphosphate; D013917:Thrombin