In vitro evaluation of ram sperm frozen with glycerol, ethylene glycol or acetamide.
The aim was to assess the in vitro effect of glycerol, ethylene glycol or acetamide on frozen-thawed ram spermatozoa. Aliquots of each sixteen ejaculates from four rams of the Morada Nova breed were diluted in Tris-egg yolk with glycerol (5%), ethylene glycol (3% or 5%) or acetamide (3% or 5%) and frozen at -196°C. After thawing, progressive sperm motility was greater (P<0.05) in cryopreservation with glycerol 5% and ethylene glycol (3% or 5%) than with acetamide (3% or 5%). Acrosome integrity was greater (P<0.05) with ethylene glycol 5% than acetamide (3% or 5%). The percentage of sperm without oxidative stress was greater (P<0.05) with ethylene glycol (3% or 5%) than with acetamide (3% or 5%). Plasma membrane integrity was greater with glycerol 5% (P<0.05) than with the other cryoprotectants. Thus, it is concluded that glycerol 5% and ethylene glycol 3% or 5% protect ram sperm against the harmful effects of freezing and that glycerol 5% offers greater protection to sperm plasma membrane.
Andrology Laboratory, Veterinary Medicine Department, Federal Rural University of Pernambuco, Recife 52171-900, PE, Brazil. silva.ecb@gmail.br
E C B
EC
Silva
J F P
JF
Cajueiro
S V
SV
Silva
A H
AH
Vidal
P C
PC
Soares
M M P
MM
Guerra
D000081:Acetamides / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D003451:Cryoprotective Agents / Q000737:chemistry / Q000494:pharmacology; D019855:Ethylene Glycol / Q000737:chemistry / Q000494:pharmacology*; D005615:Freezing; D005990:Glycerol / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D012756:Sheep / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
D000081:Acetamides; D003451:Cryoprotective Agents; C030686:acetamide; D019855:Ethylene Glycol; D005990:Glycerol