New laser soldering-based closures: a promising method in natural orifice transluminal endoscopic surgery.
BACKGROUND
Complete closure of gastrotomy is the linchpin of safe natural orifice transgastric endoscopic surgery.


OBJECTIVE
To evaluate feasibility and efficacy of a new method of gastrotomy closure by using a sutureless laser tissue-soldering (LTS) technique in an ex vivo porcine stomach.


METHODS
In vitro experiment.


METHODS
Experimental laboratory.


METHODS
Histological analysis and internal and external liquid pressure with and without hydrochloric acid exposure were determined comparing gastrotomy closure with LTS and with hand-sewn surgical sutures.


METHODS
Comparison of LTS and hand-sewn surgical gastrotomy closure. The primary outcome parameter was the internal leak pressure. Secondary parameters were the difference between internal and external leak pressures, the impact of an acid environment on the device, histological changes, and feasibility of endoscopic placement.


RESULTS
The internal liquid leak pressure after LTS was almost twice as high as after hand-sewn surgical closure (416 ± 53 mm Hg vs 229 ± 99 mm Hg; P = .01). The internal leak pressure (416 ± 53 mm Hg) after LTS was higher than the external leak pressure (154 ± 46 mm Hg; P < .0001). An acidic environment did not affect leak pressure after LTS. Endoscopic LTS closure was feasible in all experiments. Histopathology revealed only slight alterations beneath the soldering plug.


CONCLUSIONS
In vitro experiments.


CONCLUSIONS
Leak pressure after LTS closure of gastrotomy is higher than after hand-sewn surgical closure. LTS is a promising technique for closure of gastrotomies and iatrogenic perforations. Further experiments, in particular survival studies, are mandatory.
Department of Biomedical Photonics, Institute of Applied Physics, University of Bern, Bern, Switzerland.
Serge
S
Bogni
Maria-Anna
MA
Ortner
Istvan
I
Vajtai
Christian
C
Jost
Michael
M
Reinert
Bernard
B
Dallemagne
Martin
M
Frenz
D000818:Animals; D005774:Gastrostomy / Q000379:methods*; D066298:In Vitro Techniques; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D057605:Natural Orifice Endoscopic Surgery / Q000379:methods*; D010865:Pilot Projects; D011091:Polyesters; D011312:Pressure; D013270:Stomach / Q000473:pathology / Q000601:surgery*; D013537:Sutures; D013552:Swine; D058106:Wound Closure Techniques
D011091:Polyesters; C016240:polycaprolactone