In vitro and in vivo evaluation of a biphasic calcium phosphate scaffold coated with a native allogeneic extracellular matrix.
This study evaluated the osteogenic properties of biphasic calcium phosphate (BCP) scaffolds coated with extracellular matrix (ECM) derived in vitro from allogeneic mesenchymal stem cells (MSCs). BCP/ECM and plain BCP scaffolds were seeded with MSCs from F344 rats and cultured in osteoinductive medium. At 1, 7, 14 and 21 days post-seeding, assessments were made of cellularity, alkaline phosphatase (ALP) activity and RNA expression of osteocalcin, bone sialoprotein and osteopontin. MSCs seeded on BCP/ECM scaffolds exhibited significantly higher cellularity, ALP activity and transcript levels for the three genes examined. For the in vivo study, BCP/ECM and BCP scaffolds with and without MSCs were implanted subcutaneously into F344 rats. After four weeks of implantation, the extent of new bone formation and tissue response were examined by histology and histomorphometry; histological evidence showed that the seeded cell scaffolds induced new bone formation at the ectopic site and a higher average ratio of bone in the cell-seeded BCP/ECM scaffold group. Results suggest that modification of the BCP scaffold with an in vitro generated allogeneic ECM can effectively enhance osteogenic properties in vitro and in vivo.
Department of Emergency Surgery, the First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng Street, 150001, Harbin, Heilongjiang Province, China.
Shifeng
S
Zhou
Wei
W
Zhao
Xiaoqi
X
Liu
Guodan
G
Liu
Chunyang
C
Xi
Xintao
X
Wang
Jinglong
J
Yan
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D006882:Hydroxyapatites / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000201:enzymology; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000378:metabolism; D011916:Rats, Inbred F344; D054457:Tissue Scaffolds / Q000737:chemistry*; D014184:Transplantation, Homologous
D015415:Biomarkers; D020099:Coated Materials, Biocompatible; D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate; D000469:Alkaline Phosphatase