Ocular anterior segment dysgenesis upon ablation of p120 catenin in neural crest cells.
OBJECTIVE
Development of the ocular anterior segment depends largely on periocular mesenchyme cells, which are derived predominantly from neural crest cells (NCC). Specific and differential cell adhesion is expected to be instrumental in induction, migration, and differentiation of NCC. As p120 catenin (ctn) is an important component of cadherin-catenin cell adhesion complexes, we assessed its role in development of the anterior segment structure.


METHODS
We generated conditional p120ctn(fl/fl);Wnt1Cre knockout mice and studied the effect of this gene ablation on eye development in vivo. In addition, p120ctn was knocked down in vitro.


RESULTS
Wnt1Cre-mediated deletion of floxed p120ctn alleles in NCC resulted in serious ocular anterior segment dysgenesis (ASD), including iridocorneal angle closure, complete anterior chamber obliteration, iris and ciliary body hypoplasia, corneal malformation and opacity, and glaucoma-like defects. A completely penetrant phenotype was visible approximately three weeks after birth, but histologic defects were obvious at embryonal day 18.5 (E18.5). Neither migration of NCC nor expression of key transcription factors appeared to be affected. In contrast, the N-cadherin expression pattern was changed significantly in iridocorneal angle cells and corneal endothelium. A human trabecular meshwork cell line in which p120ctn was knocked down also showed decreased expression levels of N-cadherin and β-catenin at the plasma membrane, but no defect in cell migration.


CONCLUSIONS
p120ctn has a critical role in ocular mesenchyme development. Loss of p120ctn and the associated N-cadherin downregulation in NCC leads to ASD without affecting cell migration. p120ctn abnormalities might have a role in the pathophysiology of mammalian eye development.
Molecular Cell Biology Unit, Department for Molecular Biomedical Research, Ghent, Belgium.
Huiyu
H
Tian
Ellen
E
Sanders
Albert
A
Reynolds
Frans
F
van Roy
Jolanda
J
van Hengel
D000818:Animals; D000869:Anterior Eye Segment / Q000002:abnormalities / Q000473:pathology; D015820:Cadherins / Q000172:deficiency; D051177:Catenins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D002465:Cell Movement; D002478:Cells, Cultured; D015536:Down-Regulation; D005123:Eye / Q000196:embryology; D005124:Eye Abnormalities / Q000209:etiology* / Q000473:pathology; D017353:Gene Deletion; D055785:Gene Knockdown Techniques; D051379:Mice; D008822:Mice, Transgenic; D009432:Neural Crest / Q000166:cytology / Q000378:metabolism; D000095002:Delta Catenin
D015820:Cadherins; D051177:Catenins; D000095002:Delta Catenin; C000722349:Ctnnd1 protein, mouse