Liquid chromatography-tandem mass spectrometric assay for the non-nucleoside reverse transcriptase inhibitor rilpivirine in human plasma.
An analytical method based on liquid chromatographic-tandem mass spectrometry (LC-MS/MS) was developed for the determination of the non-nucleoside reverse transcriptase inhibitor rilpivirine in human plasma using nevirapine as an internal standard. Analyte and the internal standard were extracted from human plasma by liquid-liquid extraction. The reconstituted samples were chromatographed on a C(18) column using a mixture of acetonitrile and 0.1% formic acid buffer (80:20, v/v) as the mobile phase at a flow rate of 0.5 mL/min. The linearity was confirmed in the concentration range 0.51-200 ng/mL in human plasma. Multiple reaction monitoring mode was used for quantification of ion transitions at m/z 367.2/195.1 and 267.1/226.1 for the drug and the internal standard, respectively. The results of the intra- and inter-day precision and accuracy studies were well within the acceptable limits. Extraction recoveries of drug from plasma were >69.5%. A run time of 2.50 min for each sample made it possible to analyze more than 300 plasma samples per day. The developed method is simple, rapid and sensitive for the determination of rilpivirine concentrations in real-time plasma samples obtained from pharmacokinetic studies.
University College of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, India.
Laxminarayana
L
Burugula
Nageswara Rao
NR
Pilli
Ajitha
A
Makula
Durga Srinivas
DS
Lodagala
Rajnarayana
R
Kandhagatla
D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D016018:Least-Squares Analysis; D059625:Liquid-Liquid Extraction; D008297:Male; D009570:Nitriles / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D011743:Pyrimidines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D000068696:Rilpivirine; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D009570:Nitriles; D011743:Pyrimidines; D000068696:Rilpivirine