Depot-specific gene expression profiles during differentiation and transdifferentiation of bovine muscle satellite cells, and differentiation of preadipocytes.
We report a systematic study of gene expression during myogenesis and transdifferentiation in four bovine muscle tissues and of adipogenesis in three bovine fat tissues using DNA microarray analysis. One hundred hybridizations were performed and 7245 genes of known and unknown function were identified as being differentially expressed. Supervised hierarchical cluster analysis of gene expression patterns revealed the tissue specificity of genes. A close relationship in global gene expression observed for adipocyte-like cells derived from muscle and adipocytes derived from intramuscular fat suggests a common origin for these cells. The role of transthyretin in myogenesis is a novel finding. Different genes were highly induced during the transdifferentiation of myogenic satellite cells and in the adipogenesis of preadipocytes, indicating the involvement of different molecular mechanisms in these processes. Induction of CD36 and FABP4 expression in adipocyte-like cells and adipocytes may share a common pathway.
School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
Eun Ju
EJ
Lee
Hyung Jeong
HJ
Lee
Majid Rasool
MR
Kamli
Smritee
S
Pokharel
Abdul R
AR
Bhat
Yong-Ho
YH
Lee
Bong-Hwan
BH
Choi
Taehoon
T
Chun
Se Won
SW
Kang
Yong Seok
YS
Lee
Jae Woo
JW
Kim
Robert D
RD
Schnabel
Jeremy F
JF
Taylor
Inho
I
Choi
D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis; D000818:Animals; D002417:Cattle; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D005786:Gene Expression Regulation; D024510:Muscle Development; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D011228:Prealbumin / Q000235:genetics / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism; D050507:Subcutaneous Fat, Abdominal / Q000166:cytology / Q000378:metabolism; D059467:Transcriptome; D015854:Up-Regulation
D003470:Culture Media; D011228:Prealbumin