A semiquinone glucoside derivative provides protection to male reproductive system of the mice against gamma radiation toxicity.
Present investigation was carried out to evaluate the radioprotective efficacy of a novel Semiquinone glucoside derivative (SQGD), isolated from Bacillus sp. INM-1, in the male reproductive system of BALB/c mice. Animals were administered 50 mg/kg b.wt. (i.p.) SQGD 2 h before whole body γ-irradiation (10 Gy). Radiation-induced cellular toxicity and its modulation by SQGD pretreatment was evaluated in the mice testes by quantitative histological and protein expression analysis. SQGD pretreatment protects irradiated mice from radiation-induced testicular atrophy and germ cells degeneration, which may lead to emptiness of seminiferous tubules. Significant decrease in P53 and P21((Cip/WAF-1)) expression was observed in the irradiated mice pretreated (2 h) by SQGD at 6 h compared with only irradiated mice. However, contrary to P53, expressions of P21 at latter time, that is, 24-72 h was found to be increased significantly in the irradiated mice pretreated by SQGD. Significant increase in the intact PARP-1 protein expression were observed in the testes of the mice pretreated by SQGD 2 h before irradiation at 24-72 h compared with the only irradiated mice, whereas significant increase in PARP-1 cleaved fragment was noticed at 24 h. Similarly, significant increase in NF-kB and BCL-2/BAX expressions ratio was noticed in SQGD-treated mice (± irradiation) compared with irradiated mice, suggested a role of SQGD in the activation of prosurvival signaling in the testicular germinal cells population of the irradiated mice and thus contributed to protection against lethal γ-irradiation.
Institute of Nuclear Medicine and Allied Sciences, Delhi 110054, India, >
Dev Dutt
DD
Patel
Deen Dayal
DD
Bansal
Saurabh
S
Mishra
Rajesh
R
Arora
Rakesh Kumar
RK
Sharma
Swatantra Kumar
SK
Jain
Raj
R
Kumar
D000818:Animals; D001407:Bacillus / Q000737:chemistry; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D005720:Gamma Rays / Q000009:adverse effects*; D005960:Glucosides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011809:Quinones / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014916:Whole-Body Irradiation / Q000009:adverse effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism
C494827:Bax protein, mouse; C493957:Cdkn1a protein, mouse; D050759:Cyclin-Dependent Kinase Inhibitor p21; D005960:Glucosides; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D011809:Quinones; D011837:Radiation-Protective Agents; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C052617:Bcl2 protein, mouse