DNA circuits as amplifiers for the detection of nucleic acids on a paperfluidic platform.
This article describes the use of non-enzymatic nucleic acid circuits based on strand exchange reactions to detect target sequences on a paperfluidic platform. The DNA circuits that were implemented include a non-enzymatic amplifier and transduction to a fluorescent reporter; these yield an order of magnitude improvement in detection of an input nucleic acid signal. To further improve signal amplification and detection, we integrated the enzyme-free amplifier with loop-mediated isothermal amplification (LAMP). By bridging the gap between the low concentrations of LAMP amplicons and the limits of fluorescence detection, the non-enzymatic amplifier allowed us to detect as few as 1200 input templates in a paperfluidic format.
University of Texas at Austin, Chemistry and Biochemistry Dept., 1 University Station, A4800, USA.
Peter B
PB
Allen
Seyed A
SA
Arshad
Bingling
B
Li
Xi
X
Chen
Andrew D
AD
Ellington
D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D055029:Inverted Repeat Sequences; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000032:analysis*; D010209:Paper
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D004247:DNA