Outcome of adrenal tissue fragments allotransplantation: the impact of cryopreservation.
Cryopreservation is thought to have the potential to preserve tissue for transplantation. In addition, it can also be used for decreasing tissue immunogenicity, which might be important for prolonging allograft survival. In the present study we examined the impact of cryopreservation at various cooling rates on the outcome of allotransplantation of murine adrenal tissue fragments (ATFr). ATFr were cryopreserved with a cooling rate at 1; 10; 40 and more than 100 °C/min. After thawing it was found that the number of the cells expressing markers of dendritic cells (CD11c) and macrophages (CD11b) in the suspension obtained from ATFr decreased with increasing cooling rate. After allotransplantation the survival rates of adrenalectomized mice and the blood serum levels of corticosterone were higher in recipients of cryopreserved ATFr. By immunohistochemistry, cryopreserved allografts displayed a decreased infiltration by CD4+ and CD8+ T-lymphocytes as compared to fresh grafts. These findings suggest that cryopreserved allografts cause a less severe rejection by decreasing graft immunogenicity.
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine.
N M
NM
Alabedalkarim
G A
GA
Bozhok
E I
EI
Legach
V D
VD
Ustichenko
P M
PM
Zubov
S B
SB
Bilyavskaya
G V
GV
Dudetskaya
T P
TP
Bondarenko
M W
MW
Hoffmann
D000311:Adrenal Glands / Q000166:cytology / Q000276:immunology* / Q000637:transplantation*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D003345:Corticosterone / Q000097:blood; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D003713:Dendritic Cells / Q000166:cytology; D004121:Dimethyl Sulfoxide; D005802:Genes, MHC Class II; D006084:Graft Rejection; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D014184:Transplantation, Homologous
D003451:Cryoprotective Agents; D003345:Corticosterone; D004121:Dimethyl Sulfoxide