Investigation of two-photon excited fluorescence increment via crosslinked bovine serum albumin.
The two-photon excited fluorescence (TPEF) increments of two dyes via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking technique were investigated. One is Rose Bengal (RB) with a high non-radiative decay rate, while the other is Eosin Y with a low non-radiative decay rate. Experimental results demonstrate that the quantum yield and lifetime of RB are both augmented via crosslinked BSA microstructures. Compared with theoretical analysis, this result indicates that the non-radiative decay rate of RB is decreased; hence, the quenched effect induced by BSA solution is suppressed. However, the fluorescence lifetime of Eosin Y is acutely abated despite the augmented quantum yield for the two-photon crosslinking processing from BSA solution. This result deduces that the radiative decay rate increased. Furthermore, the increased TPEF intensity and lifetime of RB correlated with the concentration of fabricated crosslinked BSA microstructures through pulse selection of the employed femtosecond laser is demonstrated and capable of developing a zone-plate-like BSA microstructure.
Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.
Chun-Yu
CY
Lin
Chi-Hsiang
CH
Lien
Keng-Chi
KC
Cho
Chia-Yuan
CY
Chang
Nan-Shan
NS
Chang
Paul J
PJ
Campagnola
Chen Yuan
CY
Dong
Shean-Jen
SJ
Chen
D003432:Cross-Linking Reagents / Q000032:analysis / Q000737:chemistry; D004801:Eosine Yellowish-(YS) / Q000032:analysis / Q000737:chemistry*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D012395:Rose Bengal / Q000032:analysis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry*
D003432:Cross-Linking Reagents; D012395:Rose Bengal; D012710:Serum Albumin, Bovine; D004801:Eosine Yellowish-(YS)