[Genotype analysis of RhD-negative donors with immune antibodies].
In order to analyze the genotype of RhD-negative blood donors with immune antibodies in Harbin, the voluntary blood donors from 1 April 2008 to 30 september 2011 were detected serologically to determine the RhD-negative donors. The blood donors confirmed to be RhD negative were detected to screen the immune antibodies, the samples with immune antibodies were analyzed by PCR-SSP and DNA sequencing to detect RhD genotype. The results showed that the 12 cases of the immune antibodies (0.95%) were screened out from 1265 cases of RhD-negative donors, among which 9 cases showed anti-D-antibody, 3 cases showed anti-(D+C) antibody; 10 cases were RhD-negative, 2 cases were RHD 711D(el)C. It is concluded that RhD negative and RHD 711D(el)C are easy to be immunized to produce the immune antibodies; RhD-negative population, especially women should be highly aware of avoiding mis-transfusion of RhD-positive blood, and also avoiding multiple pregnancies resulting in newborn's hemolytic disease.
Harbin City Blood Genter, Harbin, Heilongjiang Province, China.
Chun-Yan
CY
Zhang
Ji-Hong
JH
Li
Su-Zen
SZ
Zhao
Jie
J
Liu
D001483:Base Sequence; D001782:Blood Donors; D005091:Exons; D005838:Genotype; D006801:Humans; D007518:Isoantibodies; D010641:Phenotype; D012204:Rh-Hr Blood-Group System / Q000235:genetics* / Q000276:immunology; D018029:Rho(D) Immune Globulin
D007518:Isoantibodies; C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin