The temperature response of CO2 assimilation, photochemical activities and Rubisco activation in Camelina sativa, a potential bioenergy crop with limited capacity for acclimation to heat stress.
The temperature optimum of photosynthesis coincides with the average daytime temperature in a species' native environment. Moderate heat stress occurs when temperatures exceed the optimum, inhibiting photosynthesis and decreasing productivity. In the present study, the temperature response of photosynthesis and the potential for heat acclimation was evaluated for Camelina sativa, a bioenergy crop. The temperature optimum of net CO(2) assimilation rate (A) under atmospheric conditions was 30-32 °C and was only slightly higher under non-photorespiratory conditions. The activation state of Rubisco was closely correlated with A at supra-optimal temperatures, exhibiting a parallel decrease with increasing leaf temperature. At both control and elevated temperatures, the modeled response of A to intercellular CO(2) concentration was consistent with Rubisco limiting A at ambient CO(2). Rubisco activation and photochemical activities were affected by moderate heat stress at lower temperatures in camelina than in the warm-adapted species cotton and tobacco. Growth under conditions that imposed a daily interval of moderate heat stress caused a 63 % reduction in camelina seed yield. Levels of cpn60 protein were elevated under the higher growth temperature, but acclimation of photosynthesis was minimal. Inactivation of Rubisco in camelina at temperatures above 35 °C was consistent with the temperature response of Rubisco activase activity and indicated that Rubisco activase was a prime target of inhibition by moderate heat stress in camelina. That photosynthesis exhibited no acclimation to moderate heat stress will likely impact the development of camelina and other cool season Brassicaceae as sources of bioenergy in a warmer world.
US Department of Agriculture, Agricultural Research Service, Arid-Land Agricultural Research Center, Maricopa, AZ 85138, USA.
A Elizabete
AE
Carmo-Silva
Michael E
ME
Salvucci
D000064:Acclimatization / Q000502:physiology; D056804:Biofuels; D019607:Brassicaceae / Q000254:growth & development / Q000502:physiology*; D002245:Carbon Dioxide / Q000378:metabolism; D002734:Chlorophyll / Q000032:analysis; D018556:Crops, Agricultural; D004789:Enzyme Activation; D005453:Fluorescence; D003368:Gossypium / Q000502:physiology; D018869:Heat-Shock Response; D006358:Hot Temperature; D010788:Photosynthesis / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*; D013312:Stress, Physiological; D014026:Nicotiana / Q000502:physiology
D056804:Biofuels; D010940:Plant Proteins; D002734:Chlorophyll; D002245:Carbon Dioxide; D012273:Ribulose-Bisphosphate Carboxylase