Long non-coding RNA expression profiles predict clinical phenotypes in glioma.
Glioma is the commonest form of primary brain tumor in adults with varying malignancy grades and histological subtypes. Long non-coding RNAs (lncRNAs) are a novel class of non-protein-coding transcripts that have been shown to play important roles in cancer development. To discover novel tumor-related lncRNAs and determine their associations with glioma subtypes, we first applied a lncRNA classification pipeline to identify 1970 lncRNAs that were represented on Affymetrix HG-U133 Plus 2.0 array. We then analyzed the lncRNA expression patterns in a set of previously published glioma gene expression profiles of 268 clinical specimens, and identified sets of lncRNAs that were unique to different histological subtypes (astrocytic versus oligodendroglial tumors) and malignancy grades. These lncRNAs signatures were then subject to validation in another non-overlapping, independent data set that contained 157 glioma samples. This is the first reported study that correlates lncRNA expression profiles with malignancy grade and histological differentiation in human gliomas. Our findings indicate the potential roles of lncRNAs in the biogenesis, development and differentiation of gliomas, and provide an important platform for future studies.
Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong.
Xiaoqin
X
Zhang
Stella
S
Sun
Jenny Kan Suen
JK
Pu
Anderson Chun On
AC
Tsang
Derek
D
Lee
Venus On Ying
VO
Man
Wai Man
WM
Lui
Stanley Thian Sze
ST
Wong
Gilberto Ka Kit
GK
Leung
D000328:Adult; D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D009837:Oligodendroglioma / Q000235:genetics* / Q000473:pathology; D010641:Phenotype; D062085:RNA, Long Noncoding / Q000235:genetics*
D062085:RNA, Long Noncoding