Defective macrophage migration in Gαi2- but not Gαi3-deficient mice.
Various heterotrimeric G(i) proteins are considered to be involved in cell migration and effector function of immune cells. The underlying mechanisms, how they control the activation of myeloid effector cells, are not well understood. To elucidate isoform-redundant and -specific roles for Gα(i) proteins in these processes, we analyzed mice genetically deficient in Gα(i2) or Gα(i3). First, we show an altered distribution of tissue macrophages and blood monocytes in the absence of Gα(i2) but not Gα(i3). Gα(i2)-deficient but not wild-type or Gα(i3)-deficient mice exhibited reduced recruitment of macrophages in experimental models of thioglycollate-induced peritonitis and LPS-triggered lung injury. In contrast, genetic ablation of Gα(i2) had no effect on Gα(i)-dependent peritoneal cytokine production in vitro and the phagocytosis-promoting function of the Gα(i)-coupled C5a anaphylatoxin receptor by liver macrophages in vivo. Interestingly, actin rearrangement and CCL2- and C5a anaphylatoxin receptor-induced chemotaxis but not macrophage CCR2 and C5a anaphylatoxin receptor expression were reduced in the specific absence of Gα(i2). Furthermore, knockdown of Gα(i2) caused decreased cell migration and motility of RAW 264.7 cells, which was rescued by transfection of Gα(i2) but not Gα(i3). These results indicate that Gα(i2), albeit redundant to Gα(i3) in some macrophage activation processes, clearly exhibits a Gα(i) isoform-specific role in the regulation of macrophage migration.
Clinical Department of Immunology and Rheumatology, Hannover Medical School, Hannover, Germany.
Kristina
K
Wiege
Duc D
DD
Le
Shahzad N
SN
Syed
Syed R
SR
Ali
Ana
A
Novakovic
Sandra
S
Beer-Hammer
Roland P
RP
Piekorz
Reinhold E
RE
Schmidt
Bernd
B
Nürnberg
J Engelbert
JE
Gessner
D055371:Acute Lung Injury / Q000276:immunology / Q000473:pathology; D000818:Animals; D002464:Cell Migration Inhibition / Q000235:genetics / Q000276:immunology*; D051960:GTP-Binding Protein alpha Subunit, Gi2 / Q000172:deficiency* / Q000235:genetics; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000172:deficiency* / Q000502:physiology*; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D057507:Mice, 129 Strain; D018345:Mice, Knockout; D009000:Monocytes / Q000276:immunology / Q000473:pathology; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D013864:Thioglycolates / Q000633:toxicity
D008070:Lipopolysaccharides; D013864:Thioglycolates; C498819:GNAI3 protein, human; D051960:GTP-Binding Protein alpha Subunit, Gi2; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; C498016:Gnai2 protein, mouse