[Expression and significance of vimentin in different types of chronic rhinosinusitis].
OBJECTIVE
To explore the expression and distribution of vimentin in different types of chronic rhinosinusitis and its significance.


METHODS
There were four groups including control (10 samples), Eos CRSwNP (10 samples), non-Eos CRSwNP (12 samples) and CRSsNP (10 samples). The expression of vimentin in chronic rhinosinusitis were detected by immunohistochemistry technique. The double-immunofluorescence was used to detect the positive staining of both vimentin and E-cadherin, both of which were the marker of epithelial cells.


RESULTS
The positive staining of vimentin were observed both in epithelium and lamina propria. The expression of vimentin were found in myofibroblast, endothelium and other mesenchymal cells. The vimentin positive cells in epithelium were epithelial cells but not mesenchymal cells, as they also expressed E-cadherin.


CONCLUSIONS
The vimentin positive staining cells distribute in lamina propria and epithelium of both normal nasal mucosa and chronic rhinosinusitis. The positive staining epithelial cells may generate from epithelial-mesenchymal transition. So the vimentin may play an important role in the development of chronic rhinosinusitis.
Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital,Tongji Medical College, Huazhong University of Science and Technology, Wuhan,430030, China.
Lili
L
Shi
Xiang
X
Lu
Zheng
Z
Liu
Yonghua
Y
Cui
D000328:Adult; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D002908:Chronic Disease; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism*; D012852:Sinusitis / Q000378:metabolism* / Q000473:pathology; D014746:Vimentin / Q000378:metabolism*; D055815:Young Adult
D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D014746:Vimentin