Microbial diversity, tolerance, and biodegradation potential of urban wetlands with different input regimes.
Though microbial transformations are the primary mechanism of contaminant attenuation in wetlands, much remains to be known about microbial communities in urban wetlands. In this study, the microbial communities from urban wetlands with different runoff regimes (i.e., a contaminated remnant wetland, a constructed wetland, and a remnant wetland) were assessed for their capacity to attenuate and tolerate typical urban runoff pollutants. Results from denaturing gradient gel electrophoresis of 16S rRNA genes showed relatively high similarity in community composition among the wetlands. Community-level physiological profiles had similar results but exhibited within-site variation in both the contaminated remnant and remnant wetlands. All wetland communities were less tolerant to copper than 2,4-dichlorophenoxyacetic acid; however, the contaminated remnant wetland had the highest tolerance. All study wetlands had a limited capacity to biodegrade model chlorinated aromatic compounds (e.g., 2,4-dichlorophenoxyacetic acid and 3-chlorobenzoate). Though having different input regimes and contaminant exposure histories, the study wetlands were generally similar with respect to microbial community diversity and function. Additionally, the generally low capacity for these wetlands to biodegrade mobile chlorinated organic contaminants offers preliminary insight into the limited ecosystem services these wetlands may provide in urban environments.
Faculty of Science, University of Ontario Institute of Technology, Oshawa, ON, Canada.
Nicolas
N
Gilbert
Roberta
R
Fulthorpe
Andrea E
AE
Kirkwood
D015084:2,4-Dichlorophenoxyacetic Acid / Q000633:toxicity; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D017606:Chlorine Compounds / Q000378:metabolism; D003300:Copper / Q000633:toxicity; D019015:Geologic Sediments / Q000737:chemistry; D009864:Ontario; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D014505:Urban Population; D014874:Water Pollutants, Chemical / Q000633:toxicity; D053833:Wetlands
D017606:Chlorine Compounds; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D015084:2,4-Dichlorophenoxyacetic Acid; D003300:Copper