Characterization of a novel ERF transcription factor in Artemisia annua and its induction kinetics after hormones and stress treatments.
The full-length cDNA sequence of AaERF3 was cloned and characterized from Artemisia annua. The bioinformatic analysis and phylogenetic tree analysis implied that the AaERF3 encoded a putative protein of 193 amino acids which formed a closely related subgroup with AtERF1, ERF1 and ORA59 in Arabidopsis. The result of subcellular localization showed that AaERF3 targeted to both of the nuclei and the cytoplasm. The qRT-PCR analysis showed that Green young alabastrums had the highest expression level of AaERF3 in the 5-months-old plants. The qRT-PCR analysis also revealed that ABA, Wound and Cold treatments significantly enhanced the transcript expression of AaERF3. MeJA and Ethylene treatment could also slightly induce the accumulation of AaERF3 transcription.
Plant Biotechnology Research Center, SJTU-Cornell Institute of Sustainable Agriculture and Biotechnology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
Xu
X
Lu
Weimin
W
Jiang
Ling
L
Zhang
Fangyuan
F
Zhang
Qian
Q
Shen
Tao
T
Wang
Yunfei
Y
Chen
Shaoyan
S
Wu
Zongyou
Z
Lv
Erdi
E
Gao
Bo
B
Qiu
Kexuan
K
Tang
D031172:Artemisia annua / Q000235:genetics*; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D007700:Kinetics; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D019441:Plant Epidermis / Q000166:cytology / Q000378:metabolism; D010937:Plant Growth Regulators / Q000502:physiology*; D021381:Protein Transport; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological; D014026:Nicotiana; D050656:Transcription Factor AP-2 / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
D018076:DNA, Complementary; D010937:Plant Growth Regulators; D050656:Transcription Factor AP-2