Bone marrow mesenchymal stem cells attenuate lung inflammation of hyperoxic newborn rats.
BPD is a significant global health problem and currently lacks effective therapy. We established a neonatal rat model of BPD to investigate therapeutic potential of BMSCs in neonatal lung disease. BMSCs were isolated, identified, and transfected by lentiviral vector carrying GFP gene in vitro. Neonatal rats were injected intravenously with either BMSCs or PBS after they had been already exposed to high oxygen for seven days, and assessed on post-injection day 3, day 7, and day 14 for weight gaining, lung histology, radical alveolar counts, and lung cytokine level. BMSCs were positive for CD29, CD44, and CD90 whereas negative for CD34, CD45, CD11b and with differentiation potential into osteoblasts, adipocytes, and chondrocytes. BMSCs expressed GFP after transfected by lentivirus. After injection, BMSCs exert their therapeutic benefit of improving weight gaining, preventing alveolar growth arrest, and suppressing lung inflammation of neonatal rats. Intravenous delivery of BMSCs in newborn rats conferred protection from hyperoxia-induced lung injury, and one of the effects of BMSCs treatment is suppressing lung inflammation.
Department of Pediatric, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China. zhhosh@126.com
Hongshan
H
Zhang
Jianpei
J
Fang
Haobin
H
Su
Min
M
Yang
Wenyu
W
Lai
Yougang
Y
Mai
Yanyun
Y
Wu
D017667:Adipocytes / Q000166:cytology; D000818:Animals; D000831:Animals, Newborn; D001854:Bone Marrow Cells / Q000166:cytology*; D001997:Bronchopulmonary Dysplasia / Q000473:pathology* / Q000628:therapy*; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D018496:Hyperoxia; D007231:Infant, Newborn; D007249:Inflammation / Q000628:therapy*; D019012:Integrin beta1 / Q000096:biosynthesis; D016086:Lentivirus / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018800:Thy-1 Antigens / Q000096:biosynthesis
D018960:Hyaluronan Receptors; D019012:Integrin beta1; D018800:Thy-1 Antigens; D049452:Green Fluorescent Proteins; D010100:Oxygen