Isolation and characterization of a FLOWERING LOCUS T homolog from pineapple (Ananas comosus (L.) Merr).
FLOWERING LOCUS T (FT)-like genes are crucial regulators of flowering in angiosperms. A homolog of FT, designated as AcFT (GenBank ID: HQ343233), was isolated from pineapple cultivar Comte de Paris by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The cDNA sequence of AcFT is 915 bp in length and contains an ORF of 534 bp, which encodes a protein of 177 aa. Molecular weight was 19.9 kDa and isoelectric point was 6.96. The deduced protein sequence of AcFT was 84% and 82% identical to homologs encoded by CgFT in Cymbidium goeringii and OgFT in Oncidium Gower Ramsey respectively. Quantitative real-time PCR (qRT-PCR) analyses showed that the expression of AcFT was high in flesh and none in leaves. qRT-PCR analyses in different stages indicated that the expression of AcFT reached the highest level on 40 d after flower inducing, when the multiple fruit and floral organs were forming. The 35S::AcFT transgenic Arabidopsis plants flowered earlier and had more inflorescences or branches than wild type plants.
South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang, Guangdong, 524091, China.
LingLing
L
Lv
Jun
J
Duan
JiangHui
J
Xie
ChangBin
C
Wei
YuGe
Y
Liu
ShengHui
S
Liu
GuangMing
G
Sun
D000595:Amino Acid Sequence; D031230:Ananas / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D035264:Flowers / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D056426:Genetic Loci; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D010940:Plant Proteins