Development of a host blood meal database: de novo sequencing of hemoglobin from nine small mammals using mass spectrometry.
We report the successful de novo sequencing of hemoglobin using a mass spectrometry-based approach combined with automatic data processing and manual validation for nine North American species with currently unsequenced genomes. The complete α and β chain of all nine mammalian hemoglobin samples used in this study were successfully sequenced. These sequences will be appended to the existing database containing all known hemoglobins to be used for identification of the mammalian host species that provided the last blood meal for the tick vector of Lyme disease, Ixodes scapularis.
Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ 85721, USA.
Ünige A
ÜA
Laskay
Jennifer
J
Burg
Erin J
EJ
Kaleta
Inger-Marie E
IM
Vilcins
Sam R
SR
Telford Iii
Alan G
AG
Barbour
Vicki H
VH
Wysocki
D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D030562:Databases, Protein; D006454:Hemoglobins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D020539:Sequence Analysis, Protein / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D006454:Hemoglobins