Evaluation of healing activity of PVA/chitosan hydrogels on deep second degree burn: pharmacological and toxicological tests.
Hydrogel based on poly(vinyl alcohol) containing 0.25% of chitosan was synthesized by gamma irradiation and evaluated as wound dressing material in a burn rat model. Histological analyses, Primary Irritation Index (P.I.I.) and Ocular Irritation Index (O.I.I.) were investigated. The comparative study showed that the wounds treated with PVA/chitosan hydrogel healed on the 9th day, while those treated with paraffin gauze dressing and cotton gauze healed on the 16th day. Histological analysis showed that new granulation tissue and epithelialization progressed better in wound treated with hydrogel PVA/chitosan. The determined values of P.I.I. and O.I.I. of the PVA/chitosan hydrogel were, respectively 0.5 and zero. These values indicate that the PVA/chitosan hydrogel can be considered as non-irritating to the skin.
Department of Irradiation Technology, Nuclear Research Centre of Algiers, BP-399 Alger-Gare, Algeria. nacerbou@yahoo.fr
Assia
A
Nacer Khodja
Mohamed
M
Mahlous
Djamel
D
Tahtat
Samah
S
Benamer
Souad
S
Larbi Youcef
Henni
H
Chader
Latifa
L
Mouhoub
Manel
M
Sedgelmaci
Nassima
N
Ammi
Mohamed Benslimane
MB
Mansouri
Saadia
S
Mameri
D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use* / Q000633:toxicity; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D048271:Chitosan / Q000627:therapeutic use* / Q000633:toxicity; D004195:Disease Models, Animal; D020100:Hydrogels / Q000627:therapeutic use*; D008297:Male; D011142:Polyvinyl Alcohol / Q000627:therapeutic use* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000293:injuries; D014945:Wound Healing / Q000187:drug effects*
D001672:Biocompatible Materials; D020100:Hydrogels; D011142:Polyvinyl Alcohol; D048271:Chitosan