Effect of non-surgical periodontal treatment on the subgingival microbiota of patients with chronic kidney disease.
This study investigated the effect of non-surgical periodontal therapy on the composition of subgingival microbiota of patients with chronic kidney disease (CKD). Sixteen CKD pre-dialysis individuals (CKD) and 14 individuals without clinical evidence of kidney disease (C) presenting chronic periodontitis were treated by scaling and root planing. Subgingival samples were collected from each patient and analyzed for their composition by checkerboard at baseline and 3 months post-therapy. Significant differences between groups at baseline were sought by the Mann-Whitney and χ² tests. Changes over time were examined by the Wilcoxon test. At baseline, the CKD group had significantly lower counts of E. faecalis compared to the C group (p < 0.05). After treatment, the levels of a greater number of species were reduced in the C group. Higher levels of A. israelii, C. rectus, F. periodonticum, P. micra, P. nigrescens, T. forsythia, N. mucosa, and S. anginosus (p < 0.05) were found in the CKD group compared to the C group. Also, non-responsive sites in CKD individuals harbored significantly higher levels of pathogenic species (T. forsythia, P. gingivalis, T. denticola, Fusobacterium spp., D. pneumosintes, E. faecalis and S. aureus; p < 0.05) than sites that responded to therapy, as well as non-responsive sites in the C group. The periodontitis-associated subgingival microbiota of CKD and systemically healthy individuals was similar in composition. However, high levels of pathogenic species persisted in the subgingival microbiota of patients with CKD after treatment.
Department of Dental Clinic, Division of Graduate Periodontics, School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Hilana Paula Carillo
HP
Artese
Celso Oliveira
CO
de Sousa
Maria Cynésia Medeiros de Barros
MC
Torres
Carina Maciel
CM
Silva-Boghossian
Ana Paula Vieira
AP
Colombo
D000368:Aged; D058491:Bacterial Load; D002908:Chronic Disease; D015342:DNA Probes; D012534:Dental Scaling; D005260:Female; D005881:Gingiva / Q000382:microbiology*; D006801:Humans; D008297:Male; D054892:Metagenome; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology / Q000628:therapy*; D051436:Renal Insufficiency, Chronic / Q000276:immunology / Q000382:microbiology*; D018709:Statistics, Nonparametric; D013997:Time Factors; D016896:Treatment Outcome
D015342:DNA Probes