A turn-on fluorescent sensor for imaging labile Fe(3+) in live neuronal cells at subcellular resolution.
An eye for an iron: A highly sensitive, selective and reversible turn-on Fe(3+)  sensor for imaging labile Fe(3+)  in live cells at subcellular resolution is reported. The sensor can respond to changes in intracellular Fe(3+)  levels and was used to image endogenous chelatable Fe(3+)  in live human neuroblastoma SH-SY5Y cells, with two Fe(3+)  pools being identified in mitochondria and endosomes/ lysosomes for the first time.
Department of Chemistry and Biochemistry and UMass Cranberry Health Research Center, University of Massachusetts Dartmouth, 285 Old Westport Road, Dartmouth, MA 02747, USA.
Yibin
Y
Wei
Ziya
Z
Aydin
Yi
Y
Zhang
Zhiwei
Z
Liu
Maolin
M
Guo
D045744:Cell Line, Tumor; D002470:Cell Survival; D005290:Ferric Compounds / Q000032:analysis* / Q000138:chemical synthesis; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal; D015394:Molecular Structure; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology
D005290:Ferric Compounds; D005456:Fluorescent Dyes