miR-26b promotes granulosa cell apoptosis by targeting ATM during follicular atresia in porcine ovary.
More than 99% of ovarian follicles undergo atresia in mammals, but the mechanism of follicular atresia remains to be elucidated. In this study, we explored microRNA (miRNA) regulation of follicular atresia in porcine ovary. A miRNA expression profile was constructed for healthy, early atretic, and progressively atretic follicles, and the differentially expressed miRNAs were selected and analyzed. We found that miR-26b, which was upregulated during follicular atresia, increased the number of DNA breaks and promoted granulosa cell apoptosis by targeting the ataxia telangiectasia mutated gene directly in vitro.
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Fei
F
Lin
Ran
R
Li
Zeng Xiang
ZX
Pan
Bo
B
Zhou
De Bing
DB
Yu
Xu Guang
XG
Wang
Xue Shan
XS
Ma
Jing
J
Han
Ming
M
Shen
Hong Lin
HL
Liu
D000818:Animals; D017209:Apoptosis; D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins / Q000378:metabolism*; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005496:Follicular Atresia / Q000378:metabolism*; D015571:Follicular Fluid / Q000378:metabolism; D020869:Gene Expression Profiling; D006107:Granulosa Cells / Q000166:cytology*; D049449:Luminescence; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D006080:Ovarian Follicle / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013552:Swine; D014018:Tissue Distribution; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D015854:Up-Regulation
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins; D011374:Progesterone; D004958:Estradiol; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases