Optimization of liquid fermentation of microbial consortium WSD-5 followed by saccharification and acidification of wheat straw.
The microbial consortium WSD-5 is composed of bacteria and fungi, and the cooperation and symbiosis of the contained microbes enhance the degradation ability of WSD-5. Experiment results showed that the highest cellulase and hemicellulase were obtained when ventilation volume was 4 L/min, stirring rate was 0 rpm, and substrate loading rate was 3%. After 6 days of cultivation, a 67.60% loss in wheat straw dry weight was observed. The crude enzyme secreted from WSD-5 after optimization was evaluated by experiments of saccharification and acidification. The maximum concentration of reducing sugars was 3254 mg/L after 48 h saccharification. The concentration of sCOD peaked on day 2 with a value of 4345 mg/L during acidification, and the biogas yield and methane yield were 22.3% and 32.3% higher than un-acidified samples. This study is the first attempt to explore both the saccharification and the acidification ability of crude enzymes secreted by microbial consortium.
College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, PR China.
Boting
B
Wen
Xufeng
X
Yuan
Yanzhuan
Y
Cao
Yan
Y
Liu
Xiaofen
X
Wang
Zongjun
Z
Cui
D000143:Acids / Q000737:chemistry*; D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D044822:Biodiversity; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D058645:Denaturing Gradient Gel Electrophoresis; D004798:Enzymes / Q000378:metabolism; D005285:Fermentation / Q000502:physiology*; D059013:Microbial Consortia / Q000235:genetics; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D013379:Substrate Specificity; D014908:Triticum / Q000737:chemistry*; D014866:Waste Products / Q000032:analysis*
D000143:Acids; D004798:Enzymes; D012335:RNA, Ribosomal; D014866:Waste Products