Fabrication of biocompatible titanium scaffolds using space holder technique.
Open-pore titanium scaffolds were fabricated by sintering of compressed mixtures of TiH(1.924) and urea. Spherical and irregular shaped space holders were used to investigate the effect of pore shape on cellular behavior. After removal of the space holder, the shape of the spacers was replicated to the pores. Average diameter of the pores was in the range of 300-600 μm. SEM images showed that titanium hydride resulted in higher surface roughness and larger micro porosities than pure titanium. In vitro evaluations were carried out by using MTT assay, measuring alkaline phosphatase activity and alizarin red staining in flow perfusion bioreactor for cell culture. Observations revealed excellent attachment and proliferation of G-292 cells to the highly porous scaffolds fabricated with titanium hydride and urea of this research.
Department of Materials Science and Engineering, Sharif University of Technology, PO Box 11365-9466, Tehran, Iran.
S Naddaf
SN
Dezfuli
S K
SK
Sadrnezhaad
M A
MA
Shokrgozar
S
S
Bonakdar
D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008855:Microscopy, Electron, Scanning; D054457:Tissue Scaffolds; D014025:Titanium; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D014025:Titanium; D000469:Alkaline Phosphatase