[Intestinal absorption characteristics of gastrodigenin in rats].
OBJECTIVE
To investigate the intestinal absorption characteristics of gastrodigenin.


METHODS
In vitro everted gut sac model and in situ rat single-pass intestinal perfusion model were used to evaluate the absorption characteristics of gastrodigenin in the different intestinal segments. The concentrations of gastrodigenin in the samples were determined by Ultra Performance Liquid Chromatography (UPLC) method, and the relevant absorption parameters were calculated.


RESULTS
In the everted gut sac tests, no significant difference of absorption among the four segments was observed. A positive correlation was found between drug concentration and the accumulated absorption amount (Q). At the concentration of 400 mg x L(-1), the Q of gastrodigenin in the duodenum, jejunum, ileum and colon were 224.33, 225.81, 233.18 and 189.25 microg, respectively. The in situ rat single-pass intestinal perfusion tests showed that there was also no significant difference of absorption among the four segments. The absorption rates (A) of gastrodigenin in the duodenum, jejunum, ileum and colon were 45.8%, 48.39%, 47.00%, 54.35%, respectively.


CONCLUSIONS
Gastrodigenin can be well absorbed via passive diffusion in the intestine. The absorption rates of gastrodigenin in the different intestinal segments show no regioselectivity.
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. juanhuangzi@126.com
Juan
J
Huang
Hui
H
Luo
Yun
Y
Gong
Zhongqiu
Z
Liu
Zheng
Z
Cai
D000818:Animals; D001592:Benzyl Alcohols / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D008954:Models, Biological; D010477:Perfusion / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
D001592:Benzyl Alcohols; C018966:4-hydroxybenzyl alcohol