Methods for chromatographic removal of endotoxin.
Endotoxin removal is critical when producing therapeutic proteins in bacterial systems. This hydrophobic compound can be removed through chromatography or filtration, but presents unique challenges dependent upon protein composition as well as production scale. Here we present a robust method for endotoxin removal at the pilot production scale using fast protein liquid chromatography and buffers specifically engineered for endotoxin removal.
Graduate Field of Microbiology, Cornell University, Ithaca, NY, USA. alowe@srcinc.com
Adam J
AJ
Lowe
Cameron L
CL
Bardliving
Carl A
CA
Batt
D002021:Buffers; D002473:Cell Wall / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D004731:Endotoxins / Q000737:chemistry* / Q000302:isolation & purification; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D008070:Lipopolysaccharides / Q000737:chemistry*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
D002021:Buffers; D004731:Endotoxins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40