Suppression of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation in mice by transduced Tat-Annexin protein.
We examined that the protective effects of ANX1 on 12-O-tetradecanoylphorbol- 13-acetate (TPA)-induced skin inflammation in animal models using a Tat-ANX1 protein. Topical application of the Tat-ANX1 protein markedly inhibited TPAinduced ear edema and expression levels of cyclooxygenase-2 (COX-2) as well as pro-inflammatory cytokines such as interleukin- 1 beta (IL-1 β), IL-6, and tumor necrosis factor-alpha (TNF-α). Also, application of Tat-ANX1 protein significantly inhibited nuclear translocation of nuclear factor-kappa B (NF-κ B) and phosphorylation of p38 and extracellular signalregulated kinase (ERK) mitogen-activated protein kinase (MAPK) in TPA-treated mice ears. The results indicate that Tat-ANX1 protein inhibits the inflammatory response by blocking NF-κ B and MAPK activation in TPA-induced mice ears. Therefore, the Tat-ANX1 protein may be useful as a therapeutic agent against inflammatory skin diseases.
Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 200-702, Korea.
Sun Hwa
SH
Lee
Dae Won
DW
Kim
Seon Ae
SA
Eom
Se-Young
SY
Jun
Meeyoung
M
Park
Duk-Soo
DS
Kim
Hyung Joo
HJ
Kwon
Hyeok Yil
HY
Kwon
Kyu Hyung
KH
Han
Jinseu
J
Park
Hyun Sook
HS
Hwang
Won Sik
WS
Eum
Soo Young
SY
Choi
D000818:Animals; D017302:Annexins / Q000627:therapeutic use*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D015696:Gene Products, tat / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation; D011993:Recombinant Fusion Proteins / Q000627:therapeutic use*; D012871:Skin Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D017302:Annexins; D015696:Gene Products, tat; D053583:Interleukin-1beta; D015850:Interleukin-6; D016328:NF-kappa B; D011993:Recombinant Fusion Proteins; C571041:Tat-ANX1 fusion protein; D014409:Tumor Necrosis Factor-alpha; D051546:Cyclooxygenase 2; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D013755:Tetradecanoylphorbol Acetate