[Effects of salicylic acid on synthesis of rosmarinic acid and related enzymes in the suspension cultures of Salvia miltiorrhiza].
Rosmarinic acid (RA), a phenolic acid, is one of the important secondary metabolites produced in Salvia miltiorrhiza. To observe the influence of salicylic acid (SA), an elicitor, on the synthesis of RA and related enzymes, we treated the cell suspension cultures of S. miltiorrhiza with SA and L-a-aminooxy-beta-phenylpropionic acid (AOPP), a competitive inhibitor of tyrosine aminotransferase (TAT). Under this condition, the activities of related enzymes, such as phenylalanine ammonia-lyase and TAT were traced and assayed; the accumulative amount of RA was measured. The results showed that the PAL activity reached the peak at 4 h, 124% higher than that of the control, and the content of RA reached its maximum ((5.914 +/- 0.296) mg/g dry weight) at 8 h, after treated by 6.25 mg/L SA on day 6 of the suspension culture. The results of treatment with 0.1 micromol/L AOPP showed that AOPP affected little on the TAT activity, while the PAL activity was significantly influenced, with 44% lower than that of the control at 6 h. Meanwhile, the reduced accumulation of RA ((4.709 +/- 0.204) mg/g dry weight) paralleled with the decrease in PAL activity. The co-treatment by 0.1 micromol/L AOPP and 6.25 mg/L SA relieved the restriction imposed by AOPP on PAL, and made the cell cultures accumulate more RA than sole treatment with AOPP, indicated that SA induced the accumulation of RA in suspension cell culture of S. miltiorrhiza, and the rate-limiting effect of PAL was stronger than TAT.
College of Life Sciences, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi, China.
Mengli
M
Jiao
Rongrong
R
Cao
Hongyan
H
Chen
Wenfang
W
Hao
Juan'e
J
Dong
D018929:Cell Culture Techniques / Q000379:methods*; D002934:Cinnamates / Q000378:metabolism*; D053630:Depsides / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D059828:Plant Cells / Q000378:metabolism*; D020156:Salicylic Acid / Q000494:pharmacology*; D027561:Salvia miltiorrhiza / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D013535:Suspensions; D014444:Tyrosine Transaminase / Q000378:metabolism; D000097123:Rosmarinic Acid
D002934:Cinnamates; D053630:Depsides; D010650:Phenylalanine Ammonia-Lyase; D020156:Salicylic Acid; D013535:Suspensions; D014444:Tyrosine Transaminase; D000097123:Rosmarinic Acid