RING finger palmitoylation of the endoplasmic reticulum Gp78 E3 ubiquitin ligase.
Gp78 is an E3 ubiquitin ligase within the endoplasmic reticulum-associated degradation pathway. We show that Flag-tagged gp78 undergoes sulfhydryl cysteine palmitoylation (S-palmitoylation) within the RING finger motif, responsible for its ubiquitin ligase activity. Screening of 19 palmitoyl acyl transferases (PATs) identified five that increased gp78 RING finger palmitoylation. Endoplasmic reticulum (ER)-localized Myc-DHHC6 overexpression promoted the peripheral ER distribution of Flag-gp78 while RING finger mutation and the palmitoylation inhibitor 2-bromopalmitate restricted gp78 to the central ER. Palmitoylation of RING finger cysteines therefore regulates gp78 distribution to the peripheral ER.
University of British Columbia, Department of Cellular and Physiological Sciences, Vancouver, British Columbia, Canada.
Maria
M
Fairbank
Kun
K
Huang
Alaa
A
El-Husseini
Ivan R
IR
Nabi
D020816:Amino Acid Motifs; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005786:Gene Expression Regulation; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D009154:Mutation; D010168:Palmitates / Q000737:chemistry; D010169:Palmitic Acids / Q000737:chemistry; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D060285:Receptors, Autocrine Motility Factor / Q000737:chemistry*
D010168:Palmitates; D010169:Palmitic Acids; C022776:2-bromopalmitate; C555002:Amfr protein, mouse; D060285:Receptors, Autocrine Motility Factor; D046988:Proteasome Endopeptidase Complex