[A study of immunoassay by using quantum dots to detect Hantavirus infection].
OBJECTIVE
To develop a new method to detect anti-Hantavirus IgG antibodies (HV IgG) based on quantum dots (QDs) and indirect immune technique.


METHODS
The carbodiimide crosslinking method was used to couple protein G and goat antihuman IgG on the surface of water-solubility QDs. The coverglass covered HV antigen was used as carrier, and QDs-PG-IgG conjugates was used as labeled second antibody to detect the HV-IgG in the serum samples. The detecting conditions were optimized.


RESULTS
The optimum reaction time, pH and goat antihuman IgG concentration for conjugating the QDs with goat antihuman IgG were 6.0, 2h, and 20 microg/ml, respectively. The optimum working dilution of QDs-PG-IgG conjugates was 1: 200. The detection limit of the serum samples was about 1: 1280 dilution.


CONCLUSIONS
The method established in this study has been demonstrated to be a specific, sensitive, rapid test for detecting HV antibodies, laying the foundation of single molecule detection. The anti-fluorescence quenching ability of this method was significant improved.
School of Public Health, Nanjing Medical University, Nanjing 210029, China.
Ya
Y
Shen
Jie
J
Wang
Long
L
Ba
Ping-Ping
PP
Yao
Xiao-Zhao
XZ
Deng
Chang-Jun
CJ
Wang
Han-Ping
HP
Zhu
Yun
Y
Zhang
Zhan-Qiu
ZQ
Yang
D000914:Antibodies, Viral / Q000097:blood; D005453:Fluorescence; D018778:Hantavirus Infections / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood; D045663:Quantum Dots
D000914:Antibodies, Viral; D007074:Immunoglobulin G