Detecting the effect of targeted anti-cancer medicines on single cancer cells using a poly-silicon wire ion sensor integrated with a confined sensitive window.
A mold-cast polydimethylsiloxane (PDMS) confined window was integrated with a poly-silicon wire (PSW) ion sensor. The PSW sensor surface inside the confined window was coated with a 3-aminopropyltriethoxysilane (γ-APTES) sensitive layer which allowed a single living cell to be cultivated. The change in the microenvironment due to the extracellular acidification of the single cell could then be determined by measuring the current flowing through the PSW channel. Based on this, the PSW sensor integrated with a confined sensitive window was used to detect the apoptosis as well as the effect of anti-cancer medicines on the single living non-small-lung-cancer (NSLC) cells including lung adenocarcinoma cancer cells A549 and H1299, and lung squamous-cell carcinoma CH27 cultivated inside the confined window. Single human normal cells including lung fibroblast cells WI38, lung fibroblast cells MRC5, and bronchial epithelium cell Beas-2B were tested for comparison. Two targeted anti-NSCLC cancer medicines, Iressa and Staurosporine, were used in the present study. It was found that the PSW sensor can be used to accurately detect the apoptosis of single cancer cells after the anti-cancer medicines were added. It was also found that Staurosporine is more effective than Iressa in activating the apoptosis of cancer cells.
Department of Electrical Engineering, National Chi Nan University, Puli, Nantou, Taiwan 54561, Republic of China. ylwu@ncnu.edu.tw
You-Lin
YL
Wu
Po-Yen
PY
Hsu
Chung-Ping
CP
Hsu
Jing-Jenn
JJ
Lin
D000230:Adenocarcinoma / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods; D000077156:Gefitinib; D006801:Humans; D007477:Ions / Q000378:metabolism; D008175:Lung Neoplasms / Q000473:pathology; D011437:Propylamines; D011799:Quinazolines / Q000494:pharmacology; D012821:Silanes / Q000378:metabolism; D012825:Silicon / Q000737:chemistry; D059010:Single-Cell Analysis / Q000379:methods*; D019311:Staurosporine / Q000494:pharmacology
D000970:Antineoplastic Agents; D007477:Ions; D011437:Propylamines; D011799:Quinazolines; D012821:Silanes; D019311:Staurosporine; C477625:amino-propyl-triethoxysilane; D000077156:Gefitinib; D012825:Silicon