Formation of phenolic microbial metabolites and short-chain fatty acids from rye, wheat, and oat bran and their fractions in the metabolical in vitro colon model.
Rye bran and aleurone, wheat bran and aleurone, and oat bran and cell wall concentrate were compared in their in vitro gut fermentation patterns of individual phenolic acids and short-chain fatty acids, preceded by enzymatic in vitro digestion mimicking small intestinal events. The formation of phenolic metabolites was the most pronounced from the wheat aleurone fraction. Phenylpropionic acids, presumably derived from ferulic acid (FA), were the major phenyl metabolites formed from all bran preparations. The processed rye, wheat, and oat bran fractions contained more water-extractable dietary fiber (DF) and had smaller particle sizes and were thus more easily fermentable than the corresponding brans. Rye aleurone and bran had the highest fermentation rate and extent probably due to high fructan and water-extractable arabinoxylan content. Oat samples also had a high content of water-extractable DF, β-glucan, but their fermentation rate was lower. Enzymatic digestion prior to in vitro colon fermentation changed the structure of oat cell walls as visualized by microscopy and increased the particle size, which is suggested to have retarded the fermentability of oat samples. Wheat bran was the most slowly fermentable among the studied samples, presumably due to the high proportion of water-unextractable DF. The in vitro digestion reduced the fructan content of wheat samples, thus also decreasing their fermentability. Among the studied short-chain fatty acids, acetate dominated the profiles. The highest and lowest production of propionate was from the oat and wheat samples, respectively. Interestingly, wheat aleurone generated similar amounts of butyrate as the rye fractions even without rapid gas production.
VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Finland. emilia.nordlund@vtt.fi
Emilia
E
Nordlund
Anna-Marja
AM
Aura
Ismo
I
Mattila
Tuija
T
Kössö
Xavier
X
Rouau
Kaisa
K
Poutanen
D018554:Avena / Q000737:chemistry*; D002087:Butyrates / Q000032:analysis / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000382:microbiology*; D003373:Coumaric Acids / Q000032:analysis / Q000378:metabolism; D004043:Dietary Fiber / Q000008:administration & dosage / Q000378:metabolism; D004063:Digestion; D005232:Fatty Acids, Volatile / Q000032:analysis* / Q000378:metabolism; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D005285:Fermentation; D006801:Humans; D007421:Intestine, Small / Q000378:metabolism; D054892:Metagenome; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D011422:Propionates / Q000032:analysis / Q000378:metabolism; D012434:Secale / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry*; D047071:beta-Glucans / Q000032:analysis / Q000378:metabolism
D002087:Butyrates; D003373:Coumaric Acids; D004043:Dietary Fiber; D005232:Fatty Acids, Volatile; D010636:Phenols; D011422:Propionates; D047071:beta-Glucans; C004999:ferulic acid