Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase.
Fluoride assays for oxygenases: The 2-oxoglutarate-dependent oxygenase BBOX catalyses the final step in carnitine biosynthesis and is a medicinal chemistry target. We report that BBOX can hydroxylate fluorinated substrates analogues with subsequent release of a fluoride ion, thereby enabling an efficient fluorescence-based assay.
Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
Anna M
AM
Rydzik
Ivanhoe K H
IK
Leung
Grazyna T
GT
Kochan
Armin
A
Thalhammer
Udo
U
Oppermann
Timothy D W
TD
Claridge
Christopher J
CJ
Schofield
D055162:Biocatalysis; D005453:Fluorescence; D005459:Fluorides / Q000032:analysis*; D006801:Humans; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011522:Protons; D050563:gamma-Butyrobetaine Dioxygenase / Q000032:analysis* / Q000378:metabolism*
D006845:Hydrocarbons, Fluorinated; D011522:Protons; D050563:gamma-Butyrobetaine Dioxygenase; D005459:Fluorides