Upregulation of miR-196b confers a poor prognosis in glioblastoma patients via inducing a proliferative phenotype.
OBJECTIVE
To explore the expression pattern, prognostic value and functional role of miR-196b in glioblastoma (GBM) patients using large cohorts.


METHODS
MiR-196b expression was measured using the Human v2.0 miRNA Expression BeadChip (Illumina) in 198 frozen glioma tissues. The expression levels of miR-196b were also validated in an independent cohort containing 128 formalin-fixed paraffin-embedded (FFPE) glioma samples using qRT-PCR. The presence of other molecular prognostic indicators was assessed centrally in the glioma samples. Whole genome gene profiling was performed to investigate the underlying biological behavior. MiR-196b functional analyses were performed in U87 and U251 cell lines.


RESULTS
The expression levels of miR-196b were inversely correlated with overall survival in GBM patients. Gene set enrichment analysis (GSEA) showed that the gene sets relating to cell cycle were significantly enriched in the cases with miR-196b overexpression. Functional analyses in U87 and U251 cells revealed that miR-196b was involved in cell proliferation.


CONCLUSIONS
MiR-196b is overexpressed and confers a poor prognosis via promoting cellular proliferation in GBM patients.
Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Ruimin
R
Ma
Wei
W
Yan
Guojun
G
Zhang
Hong
H
Lv
Zhizhong
Z
Liu
Fang
F
Fang
Wei
W
Zhang
Junxia
J
Zhang
Tao
T
Tao
Yongping
Y
You
Tao
T
Jiang
Xixiong
X
Kang
D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000401:mortality*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009857:Oncogenes; D010641:Phenotype; D011379:Prognosis; D015854:Up-Regulation / Q000235:genetics
C542543:MIRN196 microRNA, human; D035683:MicroRNAs