Suppressive effect of modified arabinoxylan from rice bran (MGN-3) on D-galactosamine-induced IL-18 expression and hepatitis in rats.
We investigated in this study the effect of modified arabinoxylan from rice bran (MGN-3) and its fractions on D-galactosamine (D-GalN)-induced IL-18 expression and hepatitis in rats. Male Wistar rats were pretreated with MGN-3 or fractions of the MGN-3 hydrolysate, or with saline 1 h before administering D-GalN (400 mg/kg B.W.). The serum transaminase activities, IL-18 mRNA expression level in the liver and IL-18 concentration in the serum were determined 24 h after injecting D-GalN. Both the oral and intraperitoneal administration of MGN-3 (20 mg/kg B.W.) alleviated D-GalN-induced hepatic injury under these experimental conditions. The low-molecular-weight fraction (LMW) of MGN-3 showed the strongest protective effect on D-GalN-induced liver injury, its main sugar component being glucose. Moreover, the D-GalN-induced IL-18 expression was significantly reduced by treating with MGN-3 and LMW. The results suggest that MGN-3 and LMW could provide significant protection against D-GalN liver injury, and that IL-18 might be involved in their protective influence.
Laboratory of Food and Nutrition, Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan.
Surina
S
Zheng
Hiroo
H
Sanada
Hirofumi
H
Dohi
Shizuka
S
Hirai
Yukari
Y
Egashira
D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism; D005688:Galactosamine / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D006520:Hepatitis, Animal / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D007274:Injections, Intraperitoneal; D020382:Interleukin-18 / Q000037:antagonists & inhibitors* / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D012275:Oryza / Q000737:chemistry*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D014990:Xylans / Q000494:pharmacology*
D020382:Interleukin-18; D012333:RNA, Messenger; D014990:Xylans; D005688:Galactosamine; C085118:arabinoxylan; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase