Molecular tools for carotenogenesis analysis in the zygomycete Mucor circinelloides.
The carotene producer fungus Mucor circinelloides is the zygomycete more amenable to genetic manipulations by using molecular tools. Since the initial development of an effective procedure of genetic transformation, more than two decades ago, the availability of new molecular approaches such as gene replacement techniques and gene expression inactivation by RNA silencing, in addition to the sequencing of its genome, has made Mucor a valuable organism for the study of a number of processes. Here we describe in detail the main techniques and methods currently used to manipulate M. circinelloides, including transformation, gene replacement, gene silencing, RNAi, and immunoprecipitation.
Departamento de Genética y Microbiología (Unidad Asociada al IQFR-CSIC), Facultad de Biología, Universidad de Murcia, Campus de Espinardo, Murcia, Spain. storres@um.es
Santiago
S
Torres-Martínez
Rosa M
RM
Ruiz-Vázquez
Victoriano
V
Garre
Sergio
S
López-García
Eusebio
E
Navarro
Ana
A
Vila
D015153:Blotting, Western; D002338:Carotenoids / Q000096:biosynthesis*; D004586:Electrophoresis; D020868:Gene Silencing; D005800:Genes, Fungal / Q000235:genetics; D005821:Genetic Techniques; D005822:Genetic Vectors / Q000235:genetics; D047468:Immunoprecipitation; D008970:Molecular Weight; D009089:Mucor / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011523:Protoplasts / Q000378:metabolism; D015347:RNA Probes / Q000737:chemistry; D012331:RNA, Fungal / Q000737:chemistry / Q000302:isolation & purification; D014170:Transformation, Genetic
D015347:RNA Probes; D012331:RNA, Fungal; D002338:Carotenoids