Development of a three-steps derivatization assay for the localization of double bond in monounsaturated monomers of poly-beta-hydroxyalkanoates by GC-MS.
A new gas chromatography-mass spectrometry (GC-MS) method for the localization of double bond in monounsaturated 3-hydroxyalkenoic acids monomers has been developed. A three steps derivation assay was used including a methanolysis, then acetylation and dimethyldisulfide (DMDS) addition to alkene groups. Electron impact GC-MS analysis of such derivatives offers characteristic fragments allowing the unambiguous determination of double bond position in side chain. This novel method is well-suited for the routine analysis of poly-beta-hydroxyalkanoates (PHAs), and was used to characterize monounsaturated monomers in both 3-hydroxyalkenoic acids standards as well as in mcl-PHAs and poly(3-hydroxyoctanoate-co-3-hydroxyundecenoate) (PHOU) produced by bacterial strain Pseudomonas guezennei from glucose or a mixture of sodium octanoate plus 10-undecenoic acid, respectively.
Institut Français de Recherche pour l'Exploitation de la Mer, Centre de Brest, RBE/BRM/LBMM, Plouzané, France. christelle.simon.colin@ifremer.fr
Christelle
C
Simon-Colin
Christelle
C
Gouin
Pierre
P
Lemechko
Nelly
N
Kervarec
Jean
J
Guezennec
D000107:Acetylation; D001704:Biopolymers / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008697:Methane; D008968:Molecular Conformation; D011091:Polyesters / Q000737:chemistry*
D001704:Biopolymers; D005231:Fatty Acids, Unsaturated; D011091:Polyesters; D008697:Methane