Effects of ICOSLG expressed in mouse hematological neoplasm cell lines in the GVL reaction.
As a member of the B7 family, inducible co-stimulator ligand (ICOSLG) expressed on tumor cell has been reported to have an important role in tumor immunity. In this study, we sought to determine whether the expression of ICOSLG in mouse hematological malignancy cells influences GVL reaction after mouse allogeneic BMT. In our study, we analyzed the expression of ICOSLG in six mice hematological malignancy cell lines for the first time, and found that FBL3, A20 and P388 cells expressed high levels of ICOSLG. Then, we chose A20 cells as targets to construct a GVL model and study the effects on the GVL reaction by silencing the ICOSLG gene. The survival was analyzed. We found that in GVL model, mortality of interference groups was significantly delayed compared with the control group (P=0.0005). Our results indicate that knockdown of ICOSLG of mouse leukemic cells may significantly enhance GVL effect after allogeneic BMT.
Department of Hematology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
B
B
Wang
N
N
Ma
H
H
Cheng
H
H
Zhou
H
H
Qiu
J
J
Yang
J
J
Wang
D000818:Animals; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D020544:Graft vs Leukemia Effect; D060945:Inducible T-Cell Co-Stimulator Ligand / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D007938:Leukemia / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D017254:Leukemic Infiltration / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D008099:Liver / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000473:pathology; D016019:Survival Analysis; D014184:Transplantation, Homologous
C400230:Icosl protein, mouse; D060945:Inducible T-Cell Co-Stimulator Ligand; D034741:RNA, Small Interfering; D011994:Recombinant Proteins