Bacterial adhesion forces with substratum surfaces and the susceptibility of biofilms to antibiotics.
Biofilms causing biomaterial-associated infection resist antibiotic treatment and usually necessitate the replacement of infected implants. Here we relate bacterial adhesion forces and the antibiotic susceptibility of biofilms on uncoated and polymer brush-coated silicone rubber. Nine strains of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa adhered more weakly to brush-coated silicone rubber (-0.05 ± 0.03 to -0.51 ± 0.62 nN) than to uncoated silicone rubber (-1.05 ± 0.46 to -5.1 ± 1.3 nN). Biofilms of weakly adhering organisms on polymer brush coatings remained in a planktonic state, susceptible to gentamicin, unlike biofilms formed on uncoated silicone rubber.
Department of Biomedical Engineering, W. J. Kolff Institute, University Medical Center Groningen, and University of Groningen, Groningen, The Netherlands.
Agnieszka K
AK
Muszanska
M Reza
MR
Nejadnik
Yun
Y
Chen
Edwin R
ER
van den Heuvel
Henk J
HJ
Busscher
Henny C
HC
van der Mei
Willem
W
Norde
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005839:Gentamicins / Q000494:pharmacology; D010933:Plankton / Q000187:drug effects / Q000254:growth & development; D020442:Poloxamer / Q000737:chemistry; D019736:Prostheses and Implants / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D012826:Silicone Elastomers / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000254:growth & development
D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D005839:Gentamicins; D012826:Silicone Elastomers; D020442:Poloxamer