[Mitochondrial mechanisms of antisense oligodeoxynucleotide Stat3 induced apoptosis in laryngeal carcinoma cell].
OBJECTIVE
To investigate the changes of mitochondrion by transferring antisense oligodeoxynucleotide Stat3 into laryngeal carcinoma Hep-2 cell, for elucidating the mechanism of laryngeal carcinoma Hep-2 cell apoptosis, for developing more effective treatment for laryngeal cancer.


METHODS
The designed Stat3 ASODN was transferred into laryngeal carcinoma Hep-2 cell by lipofection. Mitochondrion membrane potential, VDAC and Cyt-c were detected for determining the changes of mitochondrion.


RESULTS
MMP was fell, Cyt-c and VDAC were increased with the heighten concentration of ASODN.


CONCLUSIONS
Mitochondria approach play an important role in the apoptosis mechanism of human Hep-2 cell by Stat3. This research elucidated the regulating mechanism of Hep-2 cell proliferation by Stat3, provided a new research focus for clinical therapy.
Department of Otolaryngology-Head and Neck Surgery, Xuanwu Hospital, Capital University of Medical Science, Beijing 100053, China. lvhaili7823@gmail.com
Haili
H
Lü
Quihang
Q
Zhang
Bo
B
Yan
D017209:Apoptosis; D049109:Cell Proliferation; D045304:Cytochromes c / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism*; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics*; D050796:STAT3 Transcription Factor / Q000235:genetics*; D014162:Transfection; D050994:Voltage-Dependent Anion Channels / Q000378:metabolism
D020319:Oligodeoxyribonucleotides, Antisense; D050796:STAT3 Transcription Factor; D050994:Voltage-Dependent Anion Channels; D045304:Cytochromes c