The construction of "phylomer" peptide libraries as a rich source of potent inhibitors of protein/protein interactions.
Phylomer libraries are made from random overlapping genome fragments of biodiverse bacteria and Archaea. They provide a rich source of high-affinity binders to protein interfaces, and can be used both for target-directed screening approaches and for phenotypic screens to discover new targets. Here, we describe methods used for the construction of a Phylomer library, illustrated by examples of construction in both a yeast two-hybrid vector and a phage display vector.
Telethon Institute for Child Heath Research and Centre for Child Health, University of Western Australia, Subiaco, WA, Australia. nadiam@phylogica.com
Nadia
N
Milech
Paul
P
Watt
D001105:Archaea; D001419:Bacteria; D003001:Cloning, Molecular; D005821:Genetic Techniques; D005822:Genetic Vectors; D020745:Genome, Archaeal; D016680:Genome, Bacterial; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D054730:Protein Interaction Domains and Motifs; D011506:Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics
D019151:Peptide Library; D010455:Peptides; D011506:Proteins