Simultaneous determination of oleanolic acid, p-coumaric acid, ferulic acid, kaemperol and quercetin in rat plasma by LC-MS-MS and application to a pharmacokinetic study of Oldenlandia diffusa extract in rats.
A simple, rapid and sensitive liquid chromatography tandem mass spectrometry method is presented for the simultaneous determination of oleanolic acid, p-coumaric acid, ferulic acid, kaemperol and quercetin in rat plasma. Glycyrrhetinic acid was used as an internal standard, and sample pretreatment consisted of a liquid-liquid extraction. Chromatographic separation was achieved on a Gemini 110A C18 column (50 × 2.0 mm i.d., 5 µm) by gradient elution with a mobile phase consisting of methanol, acetonitrile and 0.01% formic acid in water. Tandem mass spectrometric detection was conducted using multiple reaction monitoring under negative ionization mode. Calibration curves offered linear ranges of two orders of magnitude with r > 0.99. The method was validated in terms of matrix effect, intra-day and inter-day precision, accuracy, linearity, specificity and stability. The relative standard deviation of intra-day and inter-day variations ranged from 2.66 to 14.74% and 1.9 to 14.55%. No substantial endogenous interference from blank plasma was observed. The method has been successfully applied to a pharmacokinetic study of Oldenlandia diffusa extract after oral administration in rats.
Guangzhou University of Chinese Medicine, Institute of Clinical Pharmacology, China.
Neng
N
Li
Changhui
C
Liu
Suiqing
S
Mi
Ningsheng
N
Wang
Xia
X
Zheng
Yingyi
Y
Li
Xiaotao
X
Huang
Shaoling
S
He
Hao
H
Chen
Xinhua
X
Xu
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D004355:Drug Stability; D044948:Flavonols / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D032067:Oldenlandia / Q000737:chemistry*; D009828:Oleanolic Acid / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods
D003373:Coumaric Acids; D044948:Flavonols; D010936:Plant Extracts; D009828:Oleanolic Acid