Estimation of biodiesel cytotoxicity by using acid phosphatase as a biomarker of lysosomal integrity.
Biodiesel is promoted as environmentally less harmful than diesel fuel. Nevertheless its water-soluble-fraction (WSF) may contain methanol, which appears by a reversion of the transesterification reaction, when biodiesel contacts water. This paper evaluated the loss of the lysosomal membrane integrity in liver homogenate of juvenils Tilapia exposed to biodiesels-WSF, through the increase of the acid phosphatase activity, as an evidence of citotoxicity. Differences in the enzyme activity levels (3.4, 2.3 and 0.8 mU mg(-1) total protein over the control value, which was 1.6 mU mg(-1) total protein), found for castor oil, waste cooking-oil and palm oil-biodiesels, respectively, were indicative of their toxicity according to this decreasing trend. WSF-chromatograms suggest the cytotoxicity as related to methanol.
LABIOMAR, Institute of Biology, Federal University of Bahia Campus Ondina, Av Oceânica 2353/403 Ondina, Salvador, Bahia, 40170-010, Brazil.
Andrea Cristina Santos
AC
da Cruz
Maria Bernadete N L
MB
Leite
Luiz Erlon Araújo
LE
Rodrigues
Iracema Andrade
IA
Nascimento
D000135:Acid Phosphatase / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D056804:Biofuels / Q000633:toxicity*; D002368:Castor Oil / Q000378:metabolism; D002849:Chromatography, Gas; D023681:Cichlids / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D004951:Esterification; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008247:Lysosomes / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism; D000073878:Palm Oil; D010938:Plant Oils / Q000378:metabolism; D018570:Risk Assessment
D056804:Biofuels; D010938:Plant Oils; D000073878:Palm Oil; D002368:Castor Oil; D000135:Acid Phosphatase; D000432:Methanol