Optimisation of synergistic biomass-degrading enzyme systems for efficient rice straw hydrolysis using an experimental mixture design.
Synergistic enzyme system for the hydrolysis of alkali-pretreated rice straw was optimised based on the synergy of crude fungal enzyme extracts with a commercial cellulase (Celluclast™). Among 13 enzyme extracts, the enzyme preparation from Aspergillus aculeatus BCC 199 exhibited the highest level of synergy with Celluclast™. This synergy was based on the complementary cellulolytic and hemicellulolytic activities of the BCC 199 enzyme extract. A mixture design was used to optimise the ternary enzyme complex based on the synergistic enzyme mixture with Bacillus subtilis expansin. Using the full cubic model, the optimal formulation of the enzyme mixture was predicted to the percentage of Celluclast™: BCC 199: expansin=41.4:37.0:21.6, which produced 769 mg reducing sugar/g biomass using 2.82 FPU/g enzymes. This work demonstrated the use of a systematic approach for the design and optimisation of a synergistic enzyme mixture of fungal enzymes and expansin for lignocellulosic degradation.
Enzyme Technology Laboratory, National Center for Genetic Engineering and Biotechnology, Thailand Science Park, Pathumthani 12120, Thailand. surisa.suw@biotec.or.th
Surisa
S
Suwannarangsee
Benjarat
B
Bunterngsook
Jantima
J
Arnthong
Atchara
A
Paemanee
Arinthip
A
Thamchaipenet
Lily
L
Eurwilaichitr
Navadol
N
Laosiripojana
Verawat
V
Champreda
D001230:Aspergillus / Q000201:enzymology*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D009097:Multienzyme Complexes / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
D009097:Multienzyme Complexes; D010936:Plant Extracts; C036909:lignocellulose; D008031:Lignin