Phenolic acids content, antioxidant and antimicrobial activity of Ligusticum mutellina L.
A simple HPLC method has been used for separation and quantitative analysis of the phenolic acids in the methanolic extracts of Ligusticum mutellina aerial parts. Chlorogenic acid was the predominant phenolic acid. Additionally, gallic, p-OH-benzoic, caffeic, p-coumaric and ferulic acids were identified. Moderate antibacterial and antifungal activity (MIC = 1.25-2.5 mg mL(-1)) was observed for the methanol extract of L. mutellina herb received from plants in flowering stage against a broad spectrum of bacteria. Micrococcus luteus, Pseudomonas aeruginosa and Candida spp. were the most sensitive to this plant material. Total phenolic content for the methanol extract of L. mutellina herb received from plants in flowering stage was 1.56 g of chlorogenic acid equivalents/100 g dry weight. The methanol extract of L. mutellina herb received from plants in flowering stage showed antioxidant activity with DPPH (IC50 value of 0.40 mg mL(-1)) and with ABTS (IC50 value of 8.65 mg mL(-1)).
Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki, PL-20-093 Lublin, Poland. elwira.sieniawska@gmail.com
Elwira
E
Sieniawska
Tomasz
T
Baj
Renata
R
Los
Krystyna
K
Skalicka-Wozniak
Anna
A
Malm
Kazimierz
K
Glowniak
D000146:Acids, Carbocyclic / Q000032:analysis* / Q000737:chemistry; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects; D002726:Chlorogenic Acid / Q000032:analysis; D035264:Flowers / Q000737:chemistry; D062385:Hydroxybenzoates / Q000032:analysis; D020128:Inhibitory Concentration 50; D030004:Ligusticum / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D016982:Micrococcus luteus / Q000187:drug effects; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects
D000146:Acids, Carbocyclic; D000890:Anti-Infective Agents; D000975:Antioxidants; D062385:Hydroxybenzoates; D010936:Plant Extracts; D002726:Chlorogenic Acid; C017616:phenolic acid