A universal platform for amplified multiplexed DNA detection based on exonuclease III-coded magnetic microparticle probes.
An amplified multiplexed DNA detection biosensor has been developed, which combines the unique cleavage function of exonuclease III (Exo III) with the separating ability of magnetic microparticles (MMPs). By using different fluorophores, the multiplexed detection of DNA is demonstrated.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Ming
M
Luo
Xia
X
Xiang
Dongshan
D
Xiang
Si
S
Yang
Xinghu
X
Ji
Zhike
Z
He
D001483:Base Sequence; D015374:Biosensing Techniques; D004279:DNA, Viral / Q000032:analysis*; D029043:Ebolavirus / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006678:HIV / Q000235:genetics*
D004279:DNA, Viral; D005456:Fluorescent Dyes; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III