[Effect of zhuhong ointment on renal antioxidant capability in skin ulcer model rats].
OBJECTIVE
To study the effect of repeated administration of Zhuhong ointment on renal antioxidant capability of ulcerous skin in rats, in order to further discuss the mechanism of mercury contained in Zhuhong ointment on the antioxidant capability of kidney in skin ulcer rats.


METHODS
Eighty SD rats were randomly divided into eight groups: Zhuhong ointment A, B, C, D, E (1.219, 0.609, 0.305, 0.152, 0.76 g x kg(-1)) groups, the vaseline group, the ulcer model group and the impairment control group. The levels of NAG and RBP of toxicity for early kidney tubular injury and T-AOC, SOD, GSH-PX and GSH in kidney were determined after consecutive administration for 14 days.


RESULTS
Compared with ulcer model group, the levels of RBP in groups A, B, C and D increased, while the levels of NAG increased only in the group A. The level of T-AOC increased in groups A, B and C. The level of T-SOD increased in the group E, while it dropped down greatly in the group A. The level of GSH-PX increased in groups A, B and C. The content of GSH increased in every dose groups.


CONCLUSIONS
Antioxidant capacity in rats can be increased in a reasonable dose of Zhuhong ointment, but some antioxidant activity can be notably inhibited by with the increase of dose.
Beijing University of Chinese Medicine, Beijing 100029, China.
Leping
L
Wang
Jianrong
J
Li
Jianxun
J
Dong
Ling
L
Luo
Rong
R
He
Bo
B
Peng
Qihua
Q
Xu
Han
H
Lin
Xuhui
X
Zhang
D000118:Acetylglucosaminidase / Q000187:drug effects / Q000652:urine; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D007684:Kidney Tubules / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D008297:Male; D008628:Mercury / Q000378:metabolism*; D009824:Ointments; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012177:Retinol-Binding Proteins / Q000187:drug effects / Q000652:urine; D012883:Skin Ulcer / Q000378:metabolism* / Q000382:microbiology; D013047:Specific Pathogen-Free Organisms; D013207:Staphylococcal Skin Infections / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D009824:Ointments; D012177:Retinol-Binding Proteins; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000118:Acetylglucosaminidase; D008628:Mercury; D005978:Glutathione