[Identification of signal transduction pathway in fresh and vitrified porcine oocytes].
Signal transduction pathway under the influence of somatotropin have been identified basis on the analysis of Ca2+ release from intracellular stores of fresh and vitrified porcine oocytes using inhibitory analysis. Somatotropin and GTP individually stimulated Ca2+ release from intracellular stores. The joint action of somatotropin and GTP activated additional Ca2+ release from intracellular stores both in fresh and vitrified porcine oocytes. Treatment of the oocytes with inhibitor of protein kinase C caused no additional Ca2+ release from intracellular stores. Ca2+ release from intracellular stores stimulated by GTP was connected with phosphate hydrolysis. Moving between intracellular Ca2+ depots stimulated by GTP was not determined by phosphate hydrolysis. Inhibitor of protein kinase C and microtubules were involved in the interaction of various intracellular depots. The data obtained suggest that signal transduction pathway in porcine oocytes do not change after vitrification.
V Iu
VIu
Denisenko
T I
TI
Kuz'mina
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D013006:Growth Hormone / Q000378:metabolism / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D015739:Nocodazole / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism* / Q000502:physiology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D015398:Signal Transduction; D013552:Swine; D058989:Vitrification
D004791:Enzyme Inhibitors; D007211:Indoles; D006160:Guanosine Triphosphate; D013006:Growth Hormone; D011493:Protein Kinase C; D015739:Nocodazole; D002118:Calcium; C064758:Ro 31-8220