Molecular characterization of the type I IFN receptor in two woodchuck species and detection of its expression in liver samples from woodchucks infected with woodchuck hepatitis virus (WHV).
Type I interferons (IFN-α/β) serve as the first line of defense against viral infection and share the same type I IFN receptor (IFNAR) complex, which is composed of IFNAR1 and -2. The Eastern woodchuck (Marmota monax) and Chinese woodchuck (Marmota himalayana) are suitable for studying hepatitis B virus (HBV) infection. Here, the complete or partial sequences of the IFNARs of both species were obtained and analyzed. Small interference RNAs targeting wIFNAR1 and -2 specifically down-regulated the expression of wIFNAR1 and -2 and the IFN-stimulated gene MxA in a woodchuck cell line, respectively. IFNAR2 was significantly up-regulated in primary woodchuck hepatocytes stimulated with IFN-α or -γ. The expression of woodchuck IFNAR1 and -2 was decreased in woodchucks chronically infected with woodchuck hepatitis virus (WHV). These results are essential for studying type I IFN-related innate immunity and therapy in hepadnaviral infection in the woodchuck model.
Division of Clinical Immunology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Hebin
H
Fan
Zhenni
Z
Zhu
Yang
Y
Wang
Xiaoyong
X
Zhang
Yinping
Y
Lu
Yuanqing
Y
Tao
Wei
W
Fan
Zhongdong
Z
Wang
Hu
H
Wang
Micheal
M
Roggendorf
Mengji
M
Lu
Baoju
B
Wang
Dongliang
D
Yang
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006509:Hepatitis B / Q000235:genetics* / Q000821:virology; D017919:Hepatitis B Virus, Woodchuck / Q000502:physiology; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D054884:Host-Pathogen Interactions; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D008099:Liver / Q000378:metabolism* / Q000821:virology; D008392:Marmota / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D064547:Myxovirus Resistance Proteins; D010802:Phylogeny; D034622:RNA Interference; D053633:Receptor, Interferon alpha-beta / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D016898:Interferon-alpha; D064547:Myxovirus Resistance Proteins; D053633:Receptor, Interferon alpha-beta; D007371:Interferon-gamma; D019204:GTP-Binding Proteins