Maspin reprograms the gene expression profile of prostate carcinoma cells for differentiation.
Maspin is an epithelial-specific tumor suppressor gene. Previous data suggest that maspin expression may redirect poorly differentiated tumor cells to better differentiated phenotypes. Further, maspin is the first and only endogenous polypeptide inhibitor of histone deacetylase 1 (HDAC1) identified thus far. In the current study, to address what central program of tumor cell redifferentiation is regulated by maspin and how tumor microenvironments further define the effects of maspin, we conducted a systematic and extensive comparison of prostate tumor cells grown in 2-dimensional culture, in 3-dimensional collagen I culture, and as in vivo bone tumors. We showed that maspin was sufficient to drive prostate tumor cells through a spectrum of temporally and spatially polarized cellular processes of redifferentiation, a reversal of epithelial-to-mesenchymal transition (EMT). Genes commonly regulated by maspin were a small subset of HDAC target genes that are closely associated with epithelial differentiation and TGFβ signaling. These results suggest that a specific endogenous HDAC inhibitor may regulate one functionally related subset of HDAC target genes, although additional maspin-induced changes of gene expression may result from tumor interaction with its specific microenvironments. Currently, EMT is recognized as a critical step in tumor progression. To this end, our current study uncovered a link between maspin and a specific mechanism of prostate epithelial differentiation that can reverse EMT.
Wayne State University School of Medicine, Detroit, MI, USA.
M Margarida
MM
Bernardo
Yonghong
Y
Meng
Jaron
J
Lockett
Gregory
G
Dyson
Alan
A
Dombkowski
Alexander
A
Kaplun
Xiaohua
X
Li
Shuping
S
Yin
Sijana
S
Dzinic
Mary
M
Olive
Ivory
I
Dean
David
D
Krass
Kamiar
K
Moin
R Daniel
RD
Bonfil
Michael
M
Cher
Wael
W
Sakr
Shijie
S
Sheng