Genetic characterization of a new subtype of Hantaan virus isolated from a hemorrhagic fever with renal syndrome (HFRS) epidemic area in Hubei Province, China.
To characterize hantaviruses currently circulating in the hemorrhagic fever with renal syndrome (HFRS) epidemic area of Hubei Province, rodents were captured and serum samples were collected from several HFRS patients. The partial S segment of the hantaviruses amplified from two serum samples had a high degree of sequence identity to the corresponding hantavirus strain isolated from Apodemus agrarius (designated as HV004). The complete S, M, and L segment sequences of HV004 were determined. The sequence identities between strain HV004 and other Hantaan viruses (HTNVs) were 83 %-90 % at the nucleotide level and 95 %-99 % at the amino acid level. Phylogenetic analysis showed that HV004 belonged to a new HTNV lineage. These data suggest the presence of a new HTNV subtype, which probably caused the HFRS cases in the endemic area of Hubei Province.
State Key Laboratory of Virology, Institute of Medical Virology, School of Medicine Wuhan University, 185 Donghu Road, Wuhan, People's Republic of China.
Jin-lin
JL
Li
Jia-xin
JX
Ling
Dong-ying
DY
Liu
Jing
J
Liu
Yuan-yuan
YY
Liu
Fei
F
Wei
Fan
F
Luo
Wen
W
Chen
Yi-hui
YH
Zhang
Hai-rong
HR
Xiong
Wei
W
Hou
Zhan-qiu
ZQ
Yang
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D058872:Epidemics; D018063:Hantaan virus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D051189:Murinae / Q000145:classification / Q000821:virology; D010802:Phylogeny; D012376:Rodent Diseases / Q000453:epidemiology / Q000821:virology; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D000914:Antibodies, Viral