Polymerase chain reaction for detection of Ureaplasma diversum from urogenital swabs in cattle in Australia.
BACKGROUND
Ureaplasma diversum has been associated with various reproductive problems in cattle, including granular vulvovaginitis, endometritis, salpingitis, early embryonic death, weak calves, decreased conception rates, balanoprosthitis, impaired spermatozoids and seminal vesiculitis in bulls.


METHODS
This study briefly outlines the use of polymerase chain reaction (PCR) for the rapid detection of U. diversum directly from urogenital swabs collected from Australian beef cattle.


RESULTS
The 16S ribosomal RNA gene sequences obtained from the PCR products of the clinical samples were closely related to U. diversum strain A417.


CONCLUSIONS
The present test enabled detection of the organism directly from clinical swabs collected from animals with or without lesions.
School of Animal and Veterinary Science, University of Adelaide, Roseworthy, South Australia 5173, Australia.
A
A
Smith
K K
KK
Chousalkar
P C
PC
Chenoweth
D000818:Animals; D001315:Australia; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D014509:Ureaplasma / Q000302:isolation & purification*; D016869:Ureaplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D014621:Vagina / Q000382:microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S