[Determination of chloramphenicol in propolis by high performance liquid chromatography-tandem mass spectrometry].
A method for the determination of chloramphenicol in propolis was developed by high performance liquid chromatography-tandem mass spectrometry. The flavones were removed with lead acetate solution after the extraction of the sample with water. The extract was cleaned up by liquid-liquid extraction. Internal standard method was used for quantitative analysis. The linear range was 0.05 - 2.0 microg/L and the correlation coefficient (r2) was 0.9996. The limit of detection (LOD, S/N = 3) and limit of quantitation (LOQ, S/N = 10) were 0.1 microg/kg and 0.3 microg/kg, respectively. The recoveries ranged from 70.1% to 94.0% while the intra-day precision lower than 10% and inter-day precision lower than 15%. The method reduced the interference by removing most of the flavones and was suitable for the determination of chloramphenicol in propolis.
National Honey Reference Laboratory, Animal, Plant and Food Inspection Center (APFIC) of Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China. zhangxy@jsciq.gov.cn
Xiaoyan
X
Zhang
Rui
R
Zhang
Wei
W
Xu
Juan
J
Huang
Yan
Y
Liu
Bin
B
Wu
Lei
L
Chen
Tao
T
Ding
Chongyu
C
Shen
Huilan
H
Chen
D000818:Animals; D002701:Chloramphenicol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D011429:Propolis / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D002701:Chloramphenicol; D011429:Propolis