Co-circulation of two genotypes of dengue virus serotype 3 in Guangzhou, China, 2009.
Dengue is emerging as the most important mosquito borne viral disease in the world. In mainland China, sporadic and large outbreaks of dengue illness caused by the four serotypes of dengue virus (DENV-1 to DENV-4) have been well documented. Guangdong province is the major affected area in China, and DENV-1 has dominantly circulated in Guangdong for a long time. In this study, a family cluster of DENV-3 infection in Guangzhou was described. Three cases were diagnosed as dengue fever based on clinical manifestation, serological and RT-PCR assays. Two DENV-3 strains were isolated in C6/36 cells and the complete genome sequences were determined. Phylogenetic analysis revealed that the new DENV-3 isolates from the family cluster were grouped within genotype III. Considering the fact that several DENV-3 strains within genotype V were also identified in Guangzhou in 2009, at least two genotypes of DENV-3 co-circulated in Guangzhou. Careful investigation and virological analysis should be warranted in the future.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No. 20 Dongda Street, Fengtai District, Beijing 100071, China.
Tao
T
Jiang
Xue-Dong
XD
Yu
Wen-Xin
WX
Hong
Wei-Ze
WZ
Zhou
Man
M
Yu
Yong-Qiang
YQ
Deng
Shun-Ya
SY
Zhu
E-De
ED
Qin
Jian
J
Wang
Cheng-Feng
CF
Qin
Fu-Chun
FC
Zhang
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D003715:Dengue / Q000453:epidemiology* / Q000473:pathology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005192:Family Health; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D000914:Antibodies, Viral; D012367:RNA, Viral