HVJ-E/importin-β hybrid vector for overcoming cytoplasmic and nuclear membranes as double barrier for non-viral gene delivery.
In order to enhance the nuclear import of the transgene, we prepared plasmid DNA/importin-β conjugates consisting of biotinylated poly(ethylenimine)s and recombinant streptavidin-fused importin-β. Hemagglutinating virus of Japan-envelope vector containing the PEI polyplex/importin-β conjugate showed high transfection efficiency not only in vitro but also in vivo. We showed that novel HVJ-E/importin-β-conjugated PEI polyplex hybrid vector could overcome plasma and nuclear membrane barriers to achieve effective transfection.
Department of Applied Chemistry & Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto, Sumiyoshi, Osaka, Japan.
Takeshi
T
Kawazu
Hiroyuki
H
Kanzaki
Atsushi
A
Uno
Hideki
H
Azuma
Takeshi
T
Nagasaki
D000818:Animals; D019921:Biotinylation; D002462:Cell Membrane / Q000378:metabolism; D004247:DNA / Q000008:administration & dosage*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D008297:Male; D051379:Mice; D041681:NIH 3T3 Cells; D009685:Nuclear Envelope / Q000378:metabolism; D010957:Plasmids; D011094:Polyethyleneimine / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000737:chemistry; D029082:Sendai virus / Q000235:genetics*; D019809:Streptavidin / Q000737:chemistry; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics; D028961:beta Karyopherins / Q000008:administration & dosage*
D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D028961:beta Karyopherins; D011094:Polyethyleneimine; D004247:DNA; D019809:Streptavidin