In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model.
For the treatment of ununited fractures, we developed a system of delivering magnetic labelled mesenchymal stromal cells (MSCs) using an extracorporeal magnetic device. In this study, we transplanted ferucarbotran-labelled and luciferase-positive bone marrow-derived MSCs into a non-healing femoral fracture rat model in the presence of a magnetic field. The biological fate of the transplanted MSCs was observed using luciferase-based bioluminescence imaging and we found that the number of MSC derived photons increased from day one to day three and thereafter decreased over time. The magnetic cell delivery system induced the accumulation of photons at the fracture site, while also retaining higher photon intensity from day three to week four. Furthermore, radiological and histological findings suggested improved callus formation and endochondral ossification. We therefore believe that this delivery system may be a promising option for bone regeneration.
Hiroshima University, Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan. akira5152001@yahoo.co.jp
A
A
Kodama
N
N
Kamei
G
G
Kamei
W
W
Kongcharoensombat
S
S
Ohkawa
A
A
Nakabayashi
M
M
Ochi
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement; D003911:Dextrans; D004195:Disease Models, Animal; D005260:Female; D005264:Femoral Fractures / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D017102:Fracture Healing / Q000502:physiology*; D005599:Fractures, Ununited / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D008163:Luminescent Measurements / Q000379:methods; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000473:pathology / Q000502:physiology; D051381:Rats; D011917:Rats, Inbred Lew
D003911:Dextrans; D058185:Magnetite Nanoparticles; C065507:ferumoxides