[Determination of multiple constituents in Wuling capsules by HPLC simultaneously].
OBJECTIVE
To establish a method for the content determination of 5-methylmellein, 5-hydroxymellein, 5-carboxylmellein and genistein in Wuling capsules simultaneously by HPLC.


METHODS
Four components were determined by HPLC on a Kromasil KR100-5C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile and 0. 2% phosphoric acid as mobile phase in a gradient elution. The flow rate was 1.0 mL x m min(-1), and the column temperature was set at 30 degrees C.


RESULTS
5-hydroxymellein showed a good linear relationship at the range of 7.86-157.2 ng, the average recovery was 101.0% with RSD 1.3%. 5-carboxylmellein showed a good linear relationship at the range of 9.57-191.4 ng, the average recovery was 98.6% with RSD 1.5%. Genistein showed a good linear relationship at the range of 28.80-576.0 ng, the average recovery was 98.6% with RSD 1.8%. 5-methylmellein showed a good linear relationship at the range of 21.46-429.2 ng, the average recovery was 99.2% with RSD 1.8%.


CONCLUSIONS
The established method is feasible and the repeatability is good. The method can be used for quality control of Wuling capsules.
Zhejiang Provincial Institute for Food and Drug Control, Hangzhou 310004, China. chenyong_zyj@163.com
Yong
Y
Chen
Jingxian
J
Lu
Ming
M
Zhu
Lei
L
Luo
D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019833:Genistein / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results
D002214:Capsules; D004365:Drugs, Chinese Herbal; D009793:Ochratoxins; C533472:wuling; D019833:Genistein; C120538:ochracin