Novel function of MKP-5/DUSP10, a phosphatase of stress-activated kinases,             on ERK-dependent gene expression, and upregulation of its gene expression in colon             carcinomas.
Mitogen-activated protein kinase phosphatase 5 (MKP-5)/DUSP10 acts as a             phosphatase of stress-activated kinases (JNK and p38), but its activity towards             ERK has not been demonstrated. In the present study we observed that MKP-5 interacts             with ERK, retains it in the cytoplasm, suppresses its activation and downregulates             ERK-dependent transcription. These data suggested a novel MKP-5 function as a             scaffold protein for the ERK pathway. We analyzed MKP-5 gene expression in several             tumors, and found that it is frequently upregulated in colorectal but not in lung             and breast cancer, suggesting its association with the malignant phenotype of             colon cancer.
Division of Cancer Chemotherapy, Miyagi Cancer Center Research                     Institute, Miyagi 981-1293, Japan.
Miyuki
M
Nomura
Ken-Ichi
K
Shiiba
Chiaki
C
Katagiri
Isao
I
Kasugai
Kouhei
K
Masuda
Ikuro
I
Sato
Masayuki
M
Sato
Yoichiro
Y
Kakugawa
Eiki
E
Nomura
Katsuhisa
K
Hayashi
Yoshihiro
Y
Nakamura
Toshiyuki
T
Nagata
Tsunayuki
T
Otsuka
Ryuichi
R
Katakura
Yoji
Y
Yamashita
Masami
M
Sato
Nobuhiro
N
Tanuma
Hiroshi
H
Shima
D002277:Carcinoma / Q000201:enzymology*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000201:enzymology*; D054637:Dual-Specificity Phosphatases / Q000235:genetics / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D020935:MAP Kinase Signaling System; D054639:Mitogen-Activated Protein Kinase Phosphatases / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D020218:Response Elements; D014158:Transcription, Genetic; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D008156:Luciferases; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C120407:DUSP10 protein, human; D054639:Mitogen-Activated Protein Kinase Phosphatases; D054637:Dual-Specificity Phosphatases