Development of lipid A-imprinted polymer hydrogels that selectively recognize lipopolysaccharides.
To remove lipopolysaccharide (LPS) from pure water, we developed polymer hydrogels that selectively recognize LPS. A molecular imprinting technique was used to prepare the polymer hydrogels. We prepared the polymer hydrogels with LPS-binding sites by using acryloyllysine and acryloylphenylalanine as functional monomers and used lipid A as a template because it is the biologically active part of LPS and contains two phosphate groups. Co-existence of n-octane during the polymerization process was highly effective in promoting the formation of LPS-accessible sites on the surface of the hydrogels. Both an electrostatic and a hydrophobic interaction between the lipid A portion of LPS and the recognition site of the imprinted hydrogel are necessary for LPS recognition. The adsorption isotherm of LPS to the lipid A-imprinted hydrogels was Langmuir-type; the saturated adsorption capacity and the adsorption constant, calculated by applying an equation for Langmuir-type adsorption isotherms, were 1.0 × 10(-11)mol/cm(2) and 2.5 × 10(5)M(-1), respectively. The imprinted hydrogels selectively recognized toxic LPS in a competition experiment in which two other kinds of LPS with similar chemical structures to that of the LPS of E. coli (toxic LPS) were adsorbed to the lipid A-imprinted hydrogels.
Graduate School of Bionics, Tokyo University of Technology, 1404-1, Katakura, Hachioji, Tokyo 192-0982, Japan.
Kei-ichi
K
Ogawa
Masumi
M
Hyuga
Tomoko
T
Okada
Norihiko
N
Minoura
D000327:Adsorption; D001665:Binding Sites; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D020100:Hydrogels / Q000737:chemistry*; D008050:Lipid A / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D008239:Lysine / Q000031:analogs & derivatives; D054802:Molecular Imprinting; D009795:Octanes / Q000737:chemistry; D010649:Phenylalanine / Q000031:analogs & derivatives; D011108:Polymers / Q000737:chemistry*; D014871:Water Microbiology; D018508:Water Purification / Q000379:methods*
D020100:Hydrogels; D008050:Lipid A; D008070:Lipopolysaccharides; D009795:Octanes; D011108:Polymers; D010649:Phenylalanine; D008239:Lysine; C026728:octane