Determining the full three-dimensional orientation of single anisotropic nanoparticles by differential interference contrast microscopy.
Keeping track: By combining differential interference contrast (DIC) image pattern recognition with DIC polarization anisotropy, the exact full three-dimensional angular information of individual tilted gold nanorods positioned in the focal plane of the objective lens can be readily determined. The angular rotational modes and kinetics of individual in-focus gold nanorods can thus be resolved dynamically.
Ames Laboratory, U.S. Department of Energy, and Department of Chemistry, Iowa State University, Ames, IA 50011, USA.
Lehui
L
Xiao
Ji Won
JW
Ha
Lin
L
Wei
Gufeng
G
Wang
Ning
N
Fang
D016880:Anisotropy; D006046:Gold / Q000032:analysis*; D053768:Metal Nanoparticles / Q000032:analysis*; D008858:Microscopy, Phase-Contrast; D010316:Particle Size
D006046:Gold