Anti-ageing effects of alpha-naphthoflavone on normal and UVB-irradiated human skin fibroblasts.
Ageing is a complex and multifactorial process resulting in several functional and aesthetic changes to the skin. We found that α-Naphthoflavone (α-NF) concentration-dependently induced pro-collagen type I protein expression and inhibited MMP-1 protein expression, in both normal and UVB-irradiated cells. SB431542 and SIS3 - inhibitors of TGF-β and Smad3, respectively - significantly alleviate α-NF-caused response of MMP-1 and pro-collagen. LY294002 (PI3K inhibitor) can reverse α-NF-induced ERK, Akt, Smad-3 activation, pro-collagen synthesis and α-NF-suppressed AP-1 activation. PD (ERK inhibitor) was not involved in pro-collagen generation and MMP-1 inhibition. We concluded that α-NF promotes pro-collagen production and inhibits MMP-1 expression via the activation of a PI3K/Akt/Smad-3 pathway in normal and UVB-irradiated human skin fibroblasts, while TGF-β may play an important role in transducing this pathway. These results suggest that α-NF, a natural plant product, has the potential to become a novel anti-ageing skin application.
Po-Lin
PL
Liao
Ching-Hao
CH
Li
Cheng-Yi
CY
Chang
Siang-Rong
SR
Lu
Cheng-Hui
CH
Lin
Ling-Shan
LS
Tse
Yu-Wen
YW
Cheng
D001571:Benzoflavones / Q000494:pharmacology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011347:Procollagen / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D015595:Skin Aging / Q000187:drug effects*; D051900:Smad3 Protein / Q000378:metabolism; D014466:Ultraviolet Rays
D001571:Benzoflavones; D024042:Collagen Type I; D011347:Procollagen; D051900:Smad3 Protein; C011512:alpha-naphthoflavone; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D020781:Matrix Metalloproteinase 1