1,3-Propanediol production from glycerol with Lactobacillus diolivorans.
The aim of this study was to evaluate the natural producer Lactobacillus diolivorans as potential production organism of 1,3-propanediol from glycerol. Different cultivation parameters, such as oxygen supply, feeding-strategy, or medium composition have been tested in batch and fed-batch cultivations. The 1,3-propanediol concentration obtained in batch cultivations was 41.7 g/l. This could be increased to 73.7 g/l in a fed-batch co-feeding glucose and glycerol with a molar ratio of 0.1. Yeast extract as part of the MRS cultivation medium could be replaced by nicotinic acid and riboflavin. Furthermore, the addition of Vitamin B(12) to the culture medium increased production by 15% to a final titer of 84.5 g/l 1,3-propanediol.
School of Bioengineering, FH Campus Wien - University of Applied Sciences, Muthgasse 62, 1190 Vienna, Austria.
Stefan
S
Pflügl
Hans
H
Marx
Diethard
D
Mattanovich
Michael
M
Sauer
D019149:Bioreactors / Q000382:microbiology*; D003198:Computer Simulation; D005990:Glycerol / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism*; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism*; D011409:Propylene Glycols / Q000302:isolation & purification* / Q000378:metabolism*
D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol; D010100:Oxygen