Comprehensive analysis of chemokines and cytokines secreted in the peritoneal cavity during laparotomy.
We recently found that chemokine-driven peritoneal cell aggregation is the primary mechanism of postoperative adhesion in a mouse model. To investigate this in humans, paired samples of peritoneal lavage fluid were obtained from seven patients immediately after incision (preoperative) and before closure (postoperative), and were assayed for the presence of 27 cytokines and chemokines using multiplex beads assay. As a result, IL-6 and CCL5 showed the most striking increase during operation. Recombinant CCL5 or lavage fluid induced chemotaxis of human peripheral blood mononuclear cells. We propose that CCL5 is possibly involved in the mechanism of postoperative adhesion in humans.
Department of Gastroenterology, Research Center for Hepatitis and Immunology, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Rei
R
Kawashima
Yuki I
YI
Kawamura
Tomoyuki
T
Oshio
Noriko
N
Mizutani
Toshihiko
T
Okada
Yutaka J
YJ
Kawamura
Fumio
F
Konishi
Taeko
T
Dohi
D000328:Adult; D000368:Aged; D001202:Ascitic Fluid / Q000737:chemistry*; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000032:analysis / Q000494:pharmacology; D018925:Chemokines / Q000032:analysis*; D002633:Chemotaxis / Q000187:drug effects; D005260:Female; D006801:Humans; D007813:Laparotomy; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000502:physiology; D008297:Male; D008875:Middle Aged; D010533:Peritoneal Lavage; D011994:Recombinant Proteins / Q000494:pharmacology
C483024:CCL5 protein, human; D018946:Chemokine CCL5; D018925:Chemokines; D011994:Recombinant Proteins