Effect of Nigella sativa seeds extracts on iNOS through antioxidant potential only: crude/total extract as molecular therapy drug.
There is general belief that only pure phytomolecules may be used as molecular therapeutic agent through one to one action. However, the traditional systems of medicine e.g. Ayurveda, uses the crude extracts, mostly water decoctions and oils, as drug. A comparative study of hexane, ethyl acetate and methanol fractions of N. Sativa seeds has been carried out on fresh rat-peritoneal-macrophage culture with reference to their role on various targets of lipopolysaccharide induced release of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) expression. The results indicated significant antioxidant potential with methanolic extract as most effective. Its mechanism of action was proposed primarily through its antioxidant potential and not through direct inhibition of other kinases, involved in its signaling cascade.
Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India. yaminiok@yahoo.com
Yamini B
YB
Tripathi
A P
AP
Chaturvedi
Nidhi
N
Pandey
D000085:Acetates / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D005591:Chemical Fractionation; D004305:Dose-Response Relationship, Drug; D006586:Hexanes / Q000737:chemistry; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D031881:Nigella sativa / Q000737:chemistry*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D012639:Seeds / Q000737:chemistry*; D013997:Time Factors
D000085:Acetates; D000975:Antioxidants; D006586:Hexanes; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide; C007650:ethyl acetate; D052247:Nitric Oxide Synthase Type II; D000432:Methanol