In vitro degradation, biocompatibility, and in vivo osteogenesis of poly(lactic-co-glycolic acid)/calcium phosphate cement scaffold with unidirectional lamellar pore structure.
The aim of this study was to investigate the in vitro degradation, cytocompatibility, and in vivo osteogenesis of poly(lactic-co-glycolic acid) (PLGA)/calcium phosphate cement (CPC) scaffold with unidirectional lamellar pore structure. CPC-based scaffold was fabricated by unidirectional freeze casting, and PLGA was used to improve the mechanical properties of the CPC-based scaffold, which covered the surface of the pore wall as coating. The in vitro degradation results demonstrated that the PLGA/CPC scaffold had good degradability. The degradation of PLGA film on the surface of the scaffold made the CPC matrix exposed, which facilitated cell response and osteogenesis. Rat bone mesenchymal stem cells (rMSCs) were seeded on the PLGA/CPC composite scaffold. Cell viability, proliferation, and differentiation on the PLGA/CPC composite scaffold were evaluated. The results showed that viable rMSCs attached on the surface of pore wall gradually penetrated into the internal pores of the scaffold as prolongation of culture time. In addition, the rMSCs seeded on the scaffold exhibited good proliferation and growing alkaline phosphatase activity. The scaffold was implanted in the defects in distal end of femora of New Zealand white rabbits. Histological evaluation indicated that the PLGA/CPC scaffold with unidirectional lamellar pore structure had good biocompatibility and effective osteogenesis. These results suggest PLGA/CPC composite scaffold with unidirectional lamellar pore structure is a promising scaffold for bone tissue engineering.
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Fupo
F
He
Jiandong
J
Ye
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001843:Bone Cements / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019245:Compressive Strength / Q000187:drug effects; D005269:Femur / Q000187:drug effects / Q000473:pathology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D019344:Lactic Acid / Q000494:pharmacology*; D008422:Materials Testing / Q000379:methods*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010012:Osteogenesis / Q000187:drug effects*; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D051381:Rats; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C020243:calcium phosphate