Oxidative stress induced in the workers of natural gas refineries, no role for GSTM1 and GSTT1 polymorphisms.
Oxidative stress has an important role in the pathophysiology of many occupational diseases. In this controlled exposure study, the intensity of oxidative stress biomarkers was assessed in the workers of natural sweet and sour gas refineries (SwGR and SoGR, respectively) and compared with controls. In addition, the role of gluthatione S-transferase M1 (GSTM1)-null and GSTT1-null polymorphisms on the intensity of oxidative stress and liver function tests (LFTs) was investigated. Blood samples were taken and measured for lipid peroxidation (LPO) level, total antioxidant capacity (TAC) and total thiol molecules (TTMs). GSTM1- and GSTT1-null genotypes were determined using polymerase chain reaction. LPO was significantly (p < 0.05) higher in the workers of SoGR. TAC was significantly lower in SwGR subjects (p < 0.001). TTMs were significantly lower in SoGR and SwGR subjects. Among LFTs, activities of aspartate aminotransferase and alanine aminotransferase but not alkaline phosphatase were elevated significantly (p < 0.001) in SoGR subjects. Multivariate linear regression revealed no association between studied polymorphisms, oxidative stress biomarkers, and LFTs. These results indicate that working in the SoGR and SwGR can lead to oxidative stress and abnormal LFTs. Continuous monitoring of natural gas workers for probable ongoing problems is therefore suggested.
Pharmaceutics Research Center, Department of Pharmacology and Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran. alimandegary@kmu.ac.ir
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A
Mandegary
M
M
Sezavar
A
A
Saeedi
B
B
Amirheidari
B
B
Naghibi
D000328:Adult; D019652:Extraction and Processing Industry; D020022:Genetic Predisposition to Disease; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008107:Liver Diseases / Q000201:enzymology / Q000209:etiology; D008111:Liver Function Tests; D008297:Male; D059206:Natural Gas / Q000009:adverse effects*; D016273:Occupational Exposure / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects*; D011110:Polymorphism, Genetic
D005982:Glutathione Transferase; D059206:Natural Gas; C413545:glutathione S-transferase T1