Cleavage and protection of locked nucleic acid-modified DNA by restriction endonucleases.
Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far. We herein for the first time report cleavage by restriction endonuclease of LNA-modified DNA oligonucleotides. The experiments revealed that RsaI is an efficient enzyme capable of recognizing and cleaving LNA-modified DNA oligonucleotides. Furthermore, introduction of LNA nucleotides protects against cleavage by the restriction endonucleases PvuII, PstI, SacI, KpnI and EcoRI.
School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
Lucile
L
Crouzier
Camille
C
Dubois
Jesper
J
Wengel
Rakesh N
RN
Veedu
D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry*
D009696:Nucleic Acids; D004247:DNA; D004262:DNA Restriction Enzymes