Protective effect of allicin against acrylamide-induced hepatocyte damage in vitro and in vivo.
Acrylamide (AA) is known to be a neurotoxic, genotoxic, and carcinogenic compound. The presence of AA in foods causes public health concerns. In our previous study, we found that allicin can effectively reduce AA content in Maillard model system. However, there has been no report on whether allicin can reduce AA-induced toxicity in vitro and in vivo. In our present study, we evaluated the protective effect of allicin against AA-induced hepatocyte damage in cultured mouse primary hepatocytes and mouse liver. Our date suggested that allicin significantly decreased the levels of maleic dialdehyde (MDA) and 8-hydroxy-desoxyguanosine (8-OHdG) both in vitro and in vivo study. Allicin also markedly increased the activity of total superoxide dismutase (SOD) and level of glutathione (GSH). The in vitro study revealed that 15 μM allicin was the optimum concentration for inhibiting AA-induced hepatocyte damage. The in vivo study revealed that 20mg/kg b.w./day allicin was the optimum dose for inhibiting AA-induced hepatocyte damage. The protective effects of allicin against AA-induced hepatocyte damage may be due to its ability to scavenge free radicals and its effective recovery of the antioxidative defense system, and its ability to block the epoxidation process of AA to GA by inhibiting P450 enzyme.
College of Quartermaster Technology, Jilin University, Changchun 130062, China.
Lulu
L
Zhang
Huanjie
H
Zhang
Yutian
Y
Miao
Sijia
S
Wu
Haiqing
H
Ye
Yuan
Y
Yuan
D000080242:8-Hydroxy-2'-Deoxyguanosine; D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002478:Cells, Cultured; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004220:Disulfides; D022781:Hepatocytes / Q000187:drug effects*; D066298:In Vitro Techniques; D015416:Maillard Reaction; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000187:drug effects; D013441:Sulfinic Acids / Q000494:pharmacology*
D004220:Disulfides; D013441:Sulfinic Acids; D020106:Acrylamide; C006452:allicin; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine