Comparison of the QIAGEN artus HIV-1 QS-RGQ test with the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 test v2.0.
BACKGROUND
Regular HIV-1 viral load monitoring is standard of care in the developed world for patients infected with HIV-1.


OBJECTIVE
Here we report a comparative evaluation of the established Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 v2.0 test (Roche Diagnostics Ltd, Burgess Hill, UK) and the new artus HIV-1 QS-RGQ test (QIAGEN Ltd, Crawley, UK).


METHODS
169 clinical EDTA-plasma samples were tested, all of known HIV-1 subtype.


RESULTS
The mean overall log(10)c/ml difference was 0.10 (QIAGEN - Roche).


CONCLUSIONS
The artus HIV-1 QS-RGQ test compared well with the Roche TaqMan HIV-1 v2.0 test, and Bland-Altman analysis showed good agreement between the two systems across a wide range of subtypes.
Department of Virology, Barts Health NHS Trust, London E1 2ES, UK.
G R
GR
Wall
D
D
Perinpanathan
D A
DA
Clark
D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic / Q000821:virology*; D012044:Regression Analysis; D019562:Viral Load / Q000379:methods*
D011933:Reagent Kits, Diagnostic