Different approaches to expressing Edwardsiella tarda antigen GAPDH in attenuated Vibrio anguillarum for multivalent fish vaccines.
With the development of gene technology, expressing heterologous antigens in attenuated bacteria has become an important strategy to design multivalent vaccines. In our previous work, an attenuated Vibrio anguillarum named MVAV6203 was developed and proven to be an efficient live vaccine candidate. In this research, we aimed to express protective antigen glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Edwardsiella tarda in attenuated Vibrio anguillarum to establish a multivalent V. anguillarum vector vaccine. Several strategies were compared between low- vs. high-copy plasmid-mediated antigen expression, in vivo-inducible vs. constitutive antigen expression and intracellular vs. surface-displaying antigen expression. Zebrafish, Danio rerio (Hamilton), was applied as the fish model to evaluate the immune protection of the V. anguillarum vector vaccine candidates. Our results demonstrated that V. anguillarum MVAV6203 (pUTatLNG40), which harbours a low-copy plasmid-loaded antigen surface display system under the control of a constitutive promoter, presented the best protective efficacy against the infection of Vibrio anguillarum (relative per cent survival, RPS = 85%) and Edwardsiella tarda (RPS = 70%).
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Y
Y
Zheng
Y
Y
Xiao
H
H
Wu
Q
Q
Wang
J
J
Xiao
Y
Y
Zhang
Q
Q
Liu
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D001428:Bacterial Vaccines / Q000235:genetics*; D020609:Edwardsiella tarda / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000401:mortality; D005393:Fish Diseases / Q000401:mortality / Q000517:prevention & control; D005399:Fishes; D015870:Gene Expression; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000235:genetics*; D014733:Vibrio / Q000235:genetics*; D014735:Vibrio Infections / Q000276:immunology / Q000401:mortality; D015027:Zebrafish / Q000276:immunology
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases