Excess genistein suppresses the synthesis of extracellular matrix in female rat mandibular condylar cartilage.
OBJECTIVE
To investigate the effect of excess genistein on the extracellular matrix in mandibular condylar cartilage of female rats in vivo.


METHODS
Female SD rats were administered through oral gavage with genistein (50 mg/kg) or placebo daily for 6 weeks. The morphological changes of temporomandibular joints were studied with HE staining. The expression of cartilage matrix compounds (aggrecan and collagen type II), estrogen-related molecules (aromatase, estradiol, ERα and ERβ) and proliferating cell nuclear antigen (PCNA) in mandibular condylar cartilage was detected using immunohistochemistry, ELISA and real-time PCR.


RESULTS
The genistein treatment significantly reduced the thickness of the posterior and middle regions of mandibular condylar cartilage, and decreased the expression of collagen type II, aggrecan and PCNA. Compared with the control group, the estradiol content and expression levels of the key estradiol-synthesizing enzyme aromatase in the genistein-treatment group were significantly decreased. The genistein treatment significantly increased the expression of ERβ, but decreased the expression of ERα.


CONCLUSIONS
Excess genistein suppresses extracellular matrix synthesis and chondrocytes proliferation, resulting in thinner mandibular condylar cartilage. These effects may be detrimental to the ability of mandibular condylar cartilage to adapt to mechanical loads.
School of Stomatology, Fourth Military Medical University, Xi'an, China.
Shi-bin
SB
Yu
Xiang-hui
XH
Xing
Guang-ying
GY
Dong
Xi-li
XL
Weng
Mei-qing
MQ
Wang
D000818:Animals; D002356:Cartilage / Q000187:drug effects* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D004958:Estradiol / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D008335:Mandibular Condyle / Q000187:drug effects* / Q000378:metabolism; D048789:Phytoestrogens / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D048789:Phytoestrogens; D004958:Estradiol; D019833:Genistein