Poly-γ-glutamic acid enhances the growth and viability of Chinese hamster ovary cells in serum-free medium.
The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection was dependent upon the molecular weight of γPGA and its concentration. When 0.05 or 0.075 % of 500 kDa γPGA was added, the cell growth and viability were almost equal to those of Pluronic F68 supplementation and were much higher than those of the control without additives. For the first time, we show that γPGA is another environmentally-friendly medium additive that can be used in place of Pluronic F68.
Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Korea.
Bok-Hwan
BH
Chun
Yong Kwon
YK
Lee
Namhyun
N
Chung
D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D016895:Culture Media, Serum-Free; D020442:Poloxamer / Q000494:pharmacology; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D013314:Stress, Mechanical; D014783:Viscosity
D016895:Culture Media, Serum-Free; C511775:poly(gamma-glutamic acid); D020442:Poloxamer; D011099:Polyglutamic Acid