Interleukin-13 peptide kinoid vaccination attenuates allergic inflammation             in a mouse model of asthma.
Asthma is an atopic disorder with increasing frequency and severity in developed             nations. Interleukin-13 (IL-13) is one of the most critical mediators of asthma             pathology. In the present study, we developed a vaccine comprised of a keyhole             limpet hemocyanin-mIL-13 heterocomplex immunogen to persistently neutralize excessive             endogenous IL-13. Our results showed that the IL-13 peptide kinoid vaccine could             induce sustained and high titer of IL-13-specific IgG when using aluminum hydroxide             as an adjuvant, and could suppress the accumulation of eosinophils as well as             IL-13 levels in bronchoalveolar lavage fluid (BALF). In addition, total IgE and             ovalbumin (OVA)-specific IgE in serum were significantly inhibited. This study             also showed that vaccination could prevent airway inflammation and epithelial             cell proliferation with goblet cell hyperplasia in a mouse model of acute asthma.             In summary, our findings suggest that the IL-13 peptide kinoid can serve as an             innovative and effective vaccine against asthma.
State Key Laboratory of Biotherapy and Cancer Center, West China                     Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan,                     P.R. China.
Qiuping
Q
Jing
Tao
T
Yin
Yang
Y
Wan
Huashan
H
Shi
Shuntao
S
Luo
Meng
M
Li
Hailong
H
Zhang
Hong
H
He
Shijia
S
Liu
Haoyu
H
Li
Yuquan
Y
Wei
Li
L
Yang
D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000517:prevention & control*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology; D005260:Female; D020397:Goblet Cells / Q000276:immunology / Q000473:pathology; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D018793:Interleukin-13 / Q000737:chemistry / Q000276:immunology*; D008679:Metaplasia; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology*; D022223:Vaccines, Subunit
D016207:Cytokines; D007074:Immunoglobulin G; D018793:Interleukin-13; D010455:Peptides; D022223:Vaccines, Subunit; D007073:Immunoglobulin E; D010047:Ovalbumin