Signal-on electrochemical Y or junction probe detection of nucleic acid.
A methylene-blue (MB)-labeled molecular beacon junction probe allows for a signal-on electrochemical detection of nucleic acids via target recycling using endonucleases. Electron transfer is reduced when the MB is intercalated in the stem of the molecular beacon, but then electron transfer from MB to a gold electrode is enhanced upon cleavage of the junction probe due to increased probability of MB approaching the electrode when attached to the more flexible ssDNA.
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Zuliang
Z
Shen
Shizuka
S
Nakayama
Steve
S
Semancik
Herman O
HO
Sintim
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods*; D004579:Electron Transport; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D008751:Methylene Blue / Q000737:chemistry*
D006046:Gold; D004247:DNA; D004262:DNA Restriction Enzymes; D008751:Methylene Blue