The gut microbiota profile is associated with insulin action in humans.
The role of the gut microbiota in the induction of metabolic diseases has now been increasingly recognized worldwide. Indeed, a specific gut microbiota has been shown to characterize lean versus obese phenotypes both in humans and mice. We have also recently demonstrated that a precise gut microbiota is associated with the host's responsiveness to a high-fat diet. Therefore, we hypothesized that insulin resistance in humans could also be linked to a specific gut microbiota. To this aim, microbial DNA and RNA were extracted from the appendix contents of insulin-resistant versus insulin-sensitive obese subjects, matched for body mass index and age, and analyzed by DNA- and RNA-DGGE. Microbial DNA analysis showed that the patients fully segregated according to their degree of insulin action. Conversely, microbial RNA investigation showed that some degree of homology still existed between insulin-sensitive and insulin-resistant patients. Quantitative trait analysis, ordinary least squares regression, principal components regression, partial least squares, canonical correlation analysis, and canonical correspondence analysis also showed a net separation of the two phenotypes analyzed. We conclude that a specific gut microbial profile is associated with insulin action in humans.
Institut National de la Santé et de la Recherche Médicale (INSERM), Toulouse, France, matteo.serino@inserm.fr.
Matteo
M
Serino
José Manuel
JM
Fernández-Real
Eduardo
E
García-Fuentes
Eduardo García
EG
Fuentes
Maribel
M
Queipo-Ortuño
José María
JM
Moreno-Navarrete
Alex
A
Sánchez
Rémy
R
Burcelin
Francisco
F
Tinahones
D000328:Adult; D016022:Case-Control Studies; D004269:DNA, Bacterial / Q000032:analysis; D004271:DNA, Fungal / Q000032:analysis; D059305:Diet, High-Fat; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance; D008297:Male; D054892:Metagenome / Q000502:physiology; D046228:Microarray Analysis; D064307:Microbiota / Q000502:physiology*; D008875:Middle Aged; D009765:Obesity / Q000150:complications / Q000378:metabolism / Q000382:microbiology; D059467:Transcriptome
D004269:DNA, Bacterial; D004271:DNA, Fungal; D007328:Insulin