[Study on the effect of realgar nanoparticles anti-adenovirus in vitro].
OBJECTIVE
This study was to establish a model that adenovirus type 3 (HAdV-3) infected on Hep-2 cell in order to explore anti-adenovirus3 (HAdV-3) effect of Chinese medicine realgar in vitro.


METHODS
Use high-energy ball milling with distilled water to prepare realgar nanoparticles. The concentration of nanometer realgar was tested by molybdenum blue staining method and realgar nanoparticles' particle size was tested on Nano Series. The technique of cell culture with ribavirin as positiv control was to observe anti-adenovirus effect through prevention, treatment and direct inactivation of three kinds of drug delivery.


RESULTS
This drug was found to be a potential inhibitor of HAdV-3 in a concentration-dependent manner with the median toxic concentration (TC50) of 0.649 microg/ml in Hep-2 Cell culture. The median inhibition concentration (IC50) was 0.255 microg/ml when drug was added before infection. The IC50 was 0.142 microg/ml when drug was added after virus infection, and it was 0.117 microg/ml as the drug was added after it mixed with virus. The therapeutic index (TI) was 2.55, 4.57 and 5.55 respectively.


CONCLUSIONS
The direct inactivation effect of realgar nanoparticles is the most evident in three drug deliveries manner with the same concentration in vitro.
Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100052, China.
Miao
M
Cheng
Cheng-Xiang
CX
Wang
Hui-Fang
HF
Wang
Bao-Di
BD
Gou
Zhen
Z
Zhu
Ming-Zhe
MZ
Wang
Hong-Ri
HR
Xu
Wen-Bo
WB
Xu
D000256:Adenoviridae / Q000187:drug effects*; D001152:Arsenicals / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D006801:Humans; D053758:Nanoparticles; D013440:Sulfides / Q000008:administration & dosage / Q000494:pharmacology*
D001152:Arsenicals; D013440:Sulfides; C058317:arsenic disulfide