SRY-positive 78, XY ovotesticular disorder of sex development in a wolf cloned by nuclear transfer.
Recently, we reported the three wolves cloning with normal karyotype from somatic cells of endangered male gray wolves (Canis lupus), but one wolf had female external genitalia. In this study, we conducted further clinical, histological, and genetic analyses. This cloned wolf had a normal uterus but developed ovotestis. Through molecular analysis of the SRY gene, a mutation in the coding sequence of SRY gene could be excluded as a cause of intersexuality. This is the first report of a cloned wolf with a 78, XY ovotesticular disorder affecting sexual development characterized by bilateral ovotestes.
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Jung Taek
JT
Kang
Hyung Jin
HJ
Kim
Hyun Ju
HJ
Oh
So Gun
SG
Hong
Jung Eun
JE
Park
Min Jung
MJ
Kim
Min Kyu
MK
Kim
Goo
G
Jang
Dae Yong
DY
Kim
Byeong Chun
BC
Lee
D000818:Animals; D019976:Cloning, Organism / Q000662:veterinary*; D005260:Female; D007621:Karyotyping; D009154:Mutation; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D050090:Ovotesticular Disorders of Sex Development / Q000473:pathology / Q000662:veterinary*; D019596:Wolves