Monitoring and visualizing of PAHs into mangrove plant by two-photon laser confocal scanning microscopy.
In this paper, we described the applications of two-photon laser confocal scanning microscopy (TPLCSM) for in situ monitoring and visualizing the localizations and movements of naphthalene, phenanthrene, and pyrene into living Aegiceras corniculata (L.) Blanco seedlings (A. corniculata). Experimental results demonstrated that all of the polycyclic aromatic hydrocarbons (PAHs) were observed entering into the root of A. corniculata and being transmitted to the stem. The transport processes and subsequent storages of the three typical PAHs into A. corniculata were similar. Further studies indicated that the transmission rates of the PAHs in A. corniculata were in the order of naphthalene>phenanthrene>pyrene. Compared with the control group, the growth of the A. corniculata was inhibited by these three specific PAHs, and the inbibitional effect of naphthalene was the most obvious (P<0.05). Furthermore, without the need for sample manipulation or modification this TPLCSM provides us a real-time tool for direct observation of organic chemicals within plants.
School of Environmental Science and Public Health, Wenzhou Medical College, Wenzhou 325035, China.
Ping
P
Wang
Tun-Hua
TH
Wu
Yong
Y
Zhang
D004784:Environmental Monitoring / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D029623:Primulaceae / Q000737:chemistry / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism*
D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical