[Significance and role of hormone detection methods in the development of endocrinology as a biological discipline].
This review covers the development of technology and methods of hormone detection in the human biological environment: from radioimmunological technique to various immune-enzyme methods. Modem ultrasensitive methods of immuneanalysis, which function on the principle of luminescent or fluorescent signal detection, are described in the article. In the recent years physiochemical ultrasensitive methods of detection using mass-spectrometry in combination with high resolution liquid chromatography are being more acknowledged and in perspective will become the leading technique of biological detection. This article also lists main achievements reached using aforesaid methods of hormone detection both in fundamental and in clinical endocrinology.
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Goncharov
D002623:Chemistry Techniques, Analytical; D002853:Chromatography, Liquid / Q000379:methods; D019963:Clinical Chemistry Tests / Q000379:methods; D004704:Endocrinology / Q000379:methods*; D006728:Hormones / Q000032:analysis* / Q000276:immunology; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D011863:Radioimmunoassay; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry
D006728:Hormones