microRNA-146a targets the L1 cell adhesion molecule and suppresses the             metastatic potential of gastric cancer.
Recent studies have shown that microRNA-146a (miR-146a) is associated with             cancer metastasis. However, the mechanisms underlying this process remain poorly             understood. In this study, we aimed to investigate the potential role of miR-146a             in gastric cancer metastasis. A wound-healing assay and a Transwell assay were             used to investigate the impact of miR-146a on the migratory and invasive abilities             of MKN-45 cells in vitro. MKN-45 cells stably expressing miR-146a or the negative             control were transplanted into nude mice through the lateral tail vein to explore             the effect of miR-146a on tumor metastasis in vivo. A luciferase reporter assay             and western blot analysis were used to identify the potential target genes. Our             results show that the overexpression of miR-146a inhibits the invasion and metastasis             of MKN-45 cells in vitro and in vivo. Furthermore, the L1 cell adhesion molecule             (L1CAM) was identified as a novel target of miR‑146a in gastric cancer. Taken             together, our results provide evidence that miR-146a suppresses gastric cancer             cell invasion and metastasis in vitro and in vivo, which may be in part due to             the downregulation of L1CAM. miR-146a may have the therapeutic potential to suppress             gastric cancer metastasis.
Department of Oncology, The Affiliated Zhongda Hospital of Southeast University, Nanjing, Jiangsu 210029, PR China.
Zhibo
Z
Hou
Haitao
H
Yin
Cong
C
Chen
Xinzheng
X
Dai
Xiaolin
X
Li
Baorui
B
Liu
Xuefeng
X
Fang
D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D017930:Genes, Reporter; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000378:metabolism*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D039842:Neural Cell Adhesion Molecule L1 / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014183:Transplantation, Heterologous
C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D039842:Neural Cell Adhesion Molecule L1