Proteomic analysis of pulmonary tissue in tail-suspended rats under simulated weightlessness.
Weightlessness affects lung function and even causes certain damages to pulmonary tissue. This study used rat tail-suspension model to simulate the physiological effects of weightlessness and investigate the alterations of lung proteome, to reveal the mechanism of lung injury under weightlessness condition. Twenty male Sprague-Dawley rats were randomly divided into two groups: tail-suspended and control. Protein samples from pulmonary tissue of tail-suspended and control groups were separated by two-dimensional (2D) gel electrophoresis and analyzed with ImageMaster 2D elite software. Differentially expressed proteins were identified by high definition mass spectrometry (HDMS) in combination with database searching. Seventeen differentially expressed proteins were identified, among which 13 proteins were upregulated, and four proteins downregulated. The functions of these identified proteins can be classified into six classes related to: metabolism, oxidative stress, cellular functions, cytoskeletal proteins, signal tranduction, and protein degradation. They are mainly related to cellular energy metabolism, stress and inflammatory response, cell injury and repair, intracellular signal transduction, and other cellular functions, playing important roles in weightlessness-induced lung injury.
Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, Beijing 100853, PR China.
Junfeng
J
Wang
Changting
C
Liu
Tianzhi
T
Li
Yang
Y
Wang
Delong
D
Wang
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D019417:Hindlimb Suspension / Q000502:physiology*; D008168:Lung / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008840:Microfilament Proteins / Q000032:analysis / Q000378:metabolism; D008954:Models, Biological; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism; D054464:Peroxiredoxins / Q000032:analysis / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D054928:Validation Studies as Topic; D018474:Weightlessness Simulation / Q000379:methods
D008840:Microfilament Proteins; D009124:Muscle Proteins; D011506:Proteins; D020543:Proteome; C051615:transgelin; C489898:Prdx1 protein, rat; D054464:Peroxiredoxins