Specific detection by the polymerase chain reaction of potentially allergenic salmonid fish residues in processed foods.
Salmonid fish is one of the allergenic items that are recommended to be labeled in the Japanese allergen-labeling system. This study develops a salmonid-specific polymerase chain reaction (PCR) method. A new primer pair, SKE-F/SKE-R, was designed to specifically detect the salmonid fish gene encoding mitochondrial DNA cytochrome b. Genomic DNAs extracted from 58 kinds of seafood and 11 kinds of processed food were individually subjected to PCR by using the primer pair, and a salmonid-specific fragment of 212 bp was only amplified in the salmonid samples and salmonid-containing processed foods. The detection limit of the PCR method was as low as 0.02 fg/µL of salmonid fish DNA (corresponding to 10 copies). There is no ELISA method for salmonid fish, making our PCR method the only reliable measure for detecting salmonid fish in processed foods.
Tokyo University of Marine Science and Technology, Japan. ishizak@kaiyodai.ac.jp
Shoichiro
S
Ishizaki
Yumiko
Y
Sakai
Takeo
T
Yano
Shigeru
S
Nakano
Toshihiro
T
Yamada
Yuji
Y
Nagashima
Kazuo
K
Shiomi
Yoshiki
Y
Nakao
Hiroshi
H
Akiyama
D000485:Allergens / Q000032:analysis*; D000818:Animals; D045303:Cytochromes b / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification*; D057140:Fast Foods / Q000032:analysis; D006801:Humans; D007564:Japan; D057230:Limit of Detection; D016133:Polymerase Chain Reaction / Q000379:methods*; D012487:Salmonidae / Q000235:genetics*; D017747:Seafood / Q000032:analysis*
D000485:Allergens; D017931:DNA Primers; D004272:DNA, Mitochondrial; D045303:Cytochromes b