Characterization of the C-terminal diglycine motif of SUMO-1/3.
A hallmark of small ubiquitin-related modifier (SUMO) is the production of a C-terminal tail containing diglycines (GGs), which are believed to be required for SUMOylation. Whether GGs are required components in SUMOylation remains unanswered experimentally. In this study we found that the SUMO-1/3-AA/-GS/-GN/-GA mutant can form sodium dodecyl sulfate (SDS)-dithiothreitol (DTT)-resistant complexes with cellular proteins, indicating that the GG motif is not strictly required for SUMOylation.
Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Japan.
Koji
K
Yamada
Miyuki
M
Muramatsu
Daiki
D
Saito
Mai
M
Sato-Oka
Masayuki
M
Saito
Taishi
T
Moriyama
Hisato
H
Saitoh
D020816:Amino Acid Motifs; D004229:Dithiothreitol / Q000737:chemistry; D015870:Gene Expression; D006033:Glycylglycine / Q000737:chemistry* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D050506:Mutant Chimeric Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D011485:Protein Binding; D025842:SUMO-1 Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D058207:Sumoylation; D014162:Transfection; D014452:Ubiquitins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D050506:Mutant Chimeric Proteins; D025842:SUMO-1 Protein; C495501:SUMO1 protein, human; C492189:SUMO3 protein, human; D014452:Ubiquitins; C485184:enhanced green fluorescent protein; D006033:Glycylglycine; D049452:Green Fluorescent Proteins; D012967:Sodium Dodecyl Sulfate; D004229:Dithiothreitol