Development of polymorphic microsatellite markers issued from pyrosequencing technology for the medicinal mushroom  Agaricus subrufescens.
The recently described procedure of microsatellite-enriched library pyrosequencing was used to isolate microsatellite loci in the gourmet and medicinal mushroom Agaricus subrufescens. Three hundred and five candidate loci containing at least one simple sequence repeats (SSR) locus and for which primers design was successful, were obtained. From a subset of 95 loci, 35 operational and polymorphic SSR markers were developed and characterized on a sample of 14 A. subrufescens genotypes from diverse origins. These SubSSR markers each displayed from two to 10 alleles with an average of 4.66 alleles per locus. The observed heterozygosity ranged from 0 to 0.71. Several multiplex combinations can be set up, making it possible to genotype up to six markers easily and simultaneously. Cross-amplification in some closely congeneric species was successful for a subset of loci. The 35 microsatellite markers developed here provide a highly valuable molecular tool to study genetic diversity and reproductive biology of A. subrufescens.
INRA, UR1264 MycSA, Villenave d'Ornon, France. mfoulong@bordeaux.inra.fr
Marie
M
Foulongne-Oriol
Cathy
C
Spataro
Magalie
M
Moinard
Delphine
D
Cabannes
Philippe
P
Callac
Jean-Michel
JM
Savoie
D000364:Agaricus / Q000145:classification* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods*; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004271:DNA, Fungal