Human circulating microRNA-1 and microRNA-126 as potential novel indicators for acute myocardial infarction.
Circulating miRNAs have been shown as promising biomarkers for various pathologic conditions. The aim of this study was to clarify that circulating miR-1 and miR-126 in human plasma might be useful as biomarkers in acute myocardial infarction (AMI). In our study, after pre-test, two candidate miRNAs were detected by using real-time RT-PCR. Cardiac troponin I (cTnI) concentrations were measured by ELISA assay in plasma from patients with AMI (n=17) and healthy subjects (n=25), simultaneously. Increased miR-1 and decreased miR-126 in plasma from patients with AMI after the onset of symptoms compared with healthy subjects were found. A remarkable finding in this study is that miR-1, miR-126 and cTnI expression levels exhibited the same trend. Our results suggest that the plasma concentrations of miR-1 and miR-126 may be useful indicators for AMI.
Department of Internal Medicine and the Institute of Hypertension, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Guangwen
G
Long
Feng
F
Wang
Quanlu
Q
Duan
Fuqiong
F
Chen
Shenglan
S
Yang
Wei
W
Gong
Yan
Y
Wang
Chen
C
Chen
Dao Wen
DW
Wang
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015415:Biomarkers; C544547:MIRN1 microRNA, human; C527111:MIRN126 microRNA, human; D035683:MicroRNAs