Proteome analysis of developing mice diastema region.
Different from humans, who have a continuous dentition of teeth, mice have only three molars and one incisor separated by a toothless region called the diastema in the hemi mandibular arch. Although tooth buds form in the embryonic diastema, they regress and do not develop into teeth. In this study, we evaluated the proteins that modulate the diastema formation through comparative analysis with molar-forming tissue by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) proteome analysis. From the comparative and semi-quantitative proteome analysis, we identified 147 up- and 173 down-regulated proteins in the diastema compared to the molar forming proteins. Based on this proteome analysis, we selected and evaluated two candidate proteins, EMERIN and RAB7A, as diastema tissue specific markers. This study provides the first list of proteins that were detected in the mouse embryonic diastema region, which will be useful to understand the mechanisms of tooth development.
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu 700-422, Korea.
Young-Mi
YM
Chae
Young-Joo
YJ
Jin
Hyeng-Soo
HS
Kim
Gi-Jeong
GJ
Gwon
Wern-Joo
WJ
Sohn
Sung-Hyun
SH
Kim
Myoung-Ok
MO
Kim
Sanggyu
S
Lee
Jo-Young
JY
Suh
Jae-Young
JY
Kim
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003970:Diastema / Q000196:embryology*; D015536:Down-Regulation; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry; D014070:Tooth / Q000196:embryology / Q000254:growth & development; D014083:Tooth Germ / Q000196:embryology / Q000378:metabolism; D015854:Up-Regulation; D020691:rab GTP-Binding Proteins / Q000378:metabolism; D000090802:rab7 GTP-Binding Proteins
D008565:Membrane Proteins; D009687:Nuclear Proteins; D020543:Proteome; C092710:emerin; D000090802:rab7 GTP-Binding Proteins; D020691:rab GTP-Binding Proteins