Large-scale mapping of branchpoints in human pre-mRNA transcripts in vivo.
We present the first large-scale identification of lariats-the transient branched introns that are released as a byproduct of pre-mRNA splicing. The locations of the branchpoints in these introns provide insight into the early steps of splicing. From this data set, we have developed a comprehensive model of 3' splice-site selection, identified new mechanisms of alternative splicing and mapped the distribution of splicing factors around branchpoints.
Laboratory of Molecular Medicine, Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA.
Allison J
AJ
Taggart
Alec M
AM
DeSimone
Janice S
JS
Shih
Madeleine E
ME
Filloux
William G
WG
Fairbrother
D006801:Humans; D007438:Introns; D012322:RNA Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
D012322:RNA Precursors; D012333:RNA, Messenger