Photochemical immobilization of cells onto a glass substrate for in situ DNA analysis.
A simple and robust method to immobilize cells onto a glass substrate is presented.  The method employs a photochemical reaction of benzophenone, which is modified on the substrate using a standard silane coupling agent, with cells.  Cells were immobilized to an area irradiated with UV light from a standard light source under an inverted microscope.  The dependence of immobilization on the light power intensity and irradiation time was investigated.  In situ DNA analysis within the immobilized cells was demonstrated using target-primed rolling circle amplification and fluorescent detection.
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo, Japan.
Naoki
N
Sasaki
Anri
A
Isu
Reina
R
Ishii
Kae
K
Sato
D001577:Benzophenones / Q000737:chemistry; D018914:Cells, Immobilized / Q000528:radiation effects*; D004272:DNA, Mitochondrial / Q000032:analysis*; D005453:Fluorescence; D005898:Glass / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010316:Particle Size; D055668:Photochemical Processes / Q000528:radiation effects; D013499:Surface Properties; D014466:Ultraviolet Rays
D001577:Benzophenones; D004272:DNA, Mitochondrial; C047723:benzophenone