MicroRNA-23b downregulates peroxiredoxin III in human prostate cancer.
To investigate the mechanism by which peroxiredoxin III (PRDX3) is altered in human prostate cancer (PCa), we used microRNA (miRNA) target prediction program and miRNA microarray to predict and identify miR-23b as a candidate miRNA that targets PRDX3. We showed that miR-23b suppresses PRDX3 protein expression in human DU145 cells under normal and hypoxic conditions. Additionally, the clinical significance of miR-23b and PRDX3 expression in PCa patients was also confirmed. In conclusion, our data suggest that the effects of PRDX3 in PCa progression may be caused by the regulation function of miR-23b, and consequently, miR-23b may be involved in the response of PCa cells to hypoxia stress.
Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First Municipal People's Hospital, Affiliated Guangzhou Medical College, Guangzhou 510180, China.
Hui-chan
HC
He
Jian-guo
JG
Zhu
Xi-bin
XB
Chen
Shan-ming
SM
Chen
Zhao-dong
ZD
Han
Qi-shan
QS
Dai
Xiao-hui
XH
Ling
Xin
X
Fu
Zhuo-yuan
ZY
Lin
Ye-han
YH
Deng
Guo-qiang
GQ
Qin
Chao
C
Cai
Jia-hong
JH
Chen
Wei-de
WD
Zhong
D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D045744:Cell Line, Tumor; D018450:Disease Progression; D018572:Disease-Free Survival; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000860:Hypoxia; D008297:Male; D035683:MicroRNAs / Q000378:metabolism / Q000502:physiology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D061105:Peroxiredoxin III / Q000096:biosynthesis*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism*
C552430:MIRN23a microRNA, human; D035683:MicroRNAs; C516115:PRDX3 protein, human; D061105:Peroxiredoxin III