Detection of hepatitis B surface antigen by target-induced aggregation monitored by dynamic light scattering.
In the current work, a one-step, washing-free, homogeneous nanosensor assay has been constructed to sensitively detect hepatitis B surface antigen (HBsAg) based on the light scattering property of gold nanoparticles (GNPs) through a sandwich model. The two nanoprobes in this study were designed by conjugating monoclonal and polyclonal hepatitis B surface antibody (HBsAb) onto the GNPs of different diameters. First, the detection behavior of the combinations of different sizes of GNPs was evaluated and the optimized combination was determined. In analyzing HBsAg in Tris-HCl buffer, such bioassay composed of GNPs of approximately 50 and 100 nm has a limit of detection (LOD) as high as 0.005 IU/ml and a dose-dependent response ranging from 0.005 to 1 IU/ml, which indicates its good diagnostic capability and provides a useful means to analyze protein biomarkers with low virus loads. Observation with transmission electron microscopy (TEM) provides direct evidence that the increase of hydrodynamic diameters resulted from the aggregation induced by immunological reactions. The bioassay also exhibits satisfactory specificity in analyzing HBsAg in serum media. Therefore, with its simple preparation, easy readout, and good stability, this bioassay has the potential to be developed into an automated and widely used biosensor assay.
Beijing Institute of Transfusion Medicine, Beijing 100850, People's Republic of China.
Xiaohui
X
Wang
Yuan
Y
Li
Dongqin
D
Quan
Jidong
J
Wang
Yuhua
Y
Zhang
Juan
J
Du
Jianchun
J
Peng
Qiuxia
Q
Fu
Yong
Y
Zhou
Shuaizheng
S
Jia
Yingli
Y
Wang
Linsheng
L
Zhan
D000042:Absorption; D015374:Biosensing Techniques / Q000379:methods*; D002021:Buffers; D006046:Gold / Q000737:chemistry; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000097:blood; D006801:Humans; D008027:Light; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000648:ultrastructure; D010316:Particle Size; D012015:Reference Standards; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet
D002021:Buffers; D006514:Hepatitis B Surface Antigens; D006046:Gold