A novel technique for in situ aggregation of Gluconobacter oxydans using bio-adhesive magnetic nanoparticles.
Here, we present a novel technique to immobilize magnetic particles onto whole Gluconobacter oxydans in situ via a synthetic adhesive biomimetic material inspired by the protein glues of marine mussels. Our approach involves simple coating of a cell adherent polydopamine film onto magnetic nanoparticles, followed by conjugation of the polydopamine-coated nanoparticles to G. oxydans which resulted in cell aggregation. After optimization, 21.3 mg (wet cell weight) G. oxydans per milligram of nanoparticle was aggregated and separated with a magnet. Importantly, the G. oxydan aggregates showed high specific activity and good reusability. The facile approach offers the potential advantages of low cost, easy cell separation, low diffusion resistance, and high efficiency. Furthermore, the approach is a convenient platform technique for magnetization of cells in situ by direct mixing of nanoparticles with a cell suspension.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Kefeng
K
Ni
Huimin
H
Lu
Cunxun
C
Wang
Kvar C L
KC
Black
Dongzhi
D
Wei
Yuhong
Y
Ren
Phillip B
PB
Messersmith
D001422:Bacterial Adhesion / Q000187:drug effects*; D018914:Cells, Immobilized / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D017743:Equipment Reuse; D020600:Gluconobacter oxydans / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009994:Osmolar Concentration; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D012965:Sodium Chloride; D013696:Temperature
D007211:Indoles; D058185:Magnetite Nanoparticles; D011108:Polymers; C568283:polydopamine; D012965:Sodium Chloride