Modified hyaluronan hydrogels support the maintenance of mouse embryonic stem cells and human induced pluripotent stem cells.
These studies provide evidence for the ability of a commercially available, defined, hyaluronan-gelatin hydrogel, HyStem-C™, to maintain both mouse embryonic stem cells (mESCs) and human induced pluripotent stem cells (hiPSCs) in culture while retaining their growth and pluripotent characteristics. Growth curve and doubling time analysis show that mESCs and hiPSCs grow at similar rates on HyStem-C™ hydrogels and mouse embryonic fibroblasts and Matrigel™, respectively. Immunocytochemistry, flow cytometry, gene expression and karyotyping reveal that both human and murine pluripotent cells retain a high level of pluripotency on the hydrogels after multiple passages. The addition of fibronectin to HyStem-C™ enabled the attachment of hiPSCs in a xeno-free, fully defined medium.
Center for Biomaterials, Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT 06030, USA.
Yongxing
Y
Liu
Lyndon F
LF
Charles
Thomas I
TI
Zarembinski
Kalle I
KI
Johnson
Sarah K
SK
Atzet
Robin L
RL
Wesselschmidt
Mark E
ME
Wight
Liisa T
LT
Kuhn
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D020100:Hydrogels; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D007621:Karyotyping; D016256:Lewis X Antigen / Q000378:metabolism; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D015415:Biomarkers; D003470:Culture Media; D005353:Fibronectins; D020100:Hydrogels; D016256:Lewis X Antigen; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D005780:Gelatin; D006820:Hyaluronic Acid