Prognostic significance of sirtuin 2 protein nuclear localization in glioma: an immunohistochemical study.
The sirtuin 2 (SIRT2) protein is a member of the sirtuin family and homologous             to Sir2 (silent information regulator 2) of Saccharomyces cerevisiae. To assess             the pathobiological significance of SIRT2 protein expression and/or subcellular             localization in human glioma, we examined SIRT2 protein expression in human gliomas             using a polyclonal anti-SIRT2 antibody and immunohistochemistry. In this study,             samples from 23 patients with glioblastoma (GB, grade IV), 8 patients with diffuse             astrocytoma (DA, grade II) and 5 healthy individuals were examined. We established             a SIRT2 labeling index (SIRT2-LI) that represents the percentage of cells with             SIRT2 localized to the nucleus. The mean SIRT2-LI was 65.8±18.6 in GB samples,             41.2±22.8 in DA samples, and 28.6±12.3 in normal control samples. The SIRT2-LI             of GB samples was significantly higher than that of normal control samples (P<0.01,             Mann-Whitney's U-test) and that of DA samples (P<0.05). Moreover, the SIRT2-LI             was positively correlated with malignant progression. Specifically, samples from             patients with GB were divided into two groups, low SIRT2-LI (<60%) and high             SIRT2-LI (≥60%), and the patients with low SIRT2-LI samples survived significantly             longer than patients with high SIRT2-LI samples (P<0.05, Kaplan-Meier method             and log-rank test). In conclusion, SIRT2-LI was indicative of glioma malignancy,             and it may be predictive of GB patient survival.
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, and Department of Surgical Pathology, Toyama University Hospital, Yonago, Tottori 683-8503, Japan.
Natsuko
N
Imaoka
Masaharu
M
Hiratsuka
Mitsuhiko
M
Osaki
Hideki
H
Kamitani
Atsushi
A
Kambe
Junya
J
Fukuoka
Masanori
M
Kurimoto
Shoichi
S
Nagai
Futoshi
F
Okada
Takashi
T
Watanabe
Eisaku
E
Ohama
Shinsuke
S
Kato
Mitsuo
M
Oshimura
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002467:Cell Nucleus / Q000378:metabolism*; D005260:Female; D005909:Glioblastoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D011379:Prognosis; D056565:Sirtuin 2 / Q000378:metabolism*; D046888:Tissue Array Analysis; D055815:Young Adult
C474199:SIRT2 protein, human; D056565:Sirtuin 2