Degradation of the resin-dentin bonds after simulated and inhibited cariogenic challenge in an in situ model.
The aim of this study was to evaluate the resin-dentin bonds of two simplified etch-and-rinse adhesive after simulated cariogenic and inhibited cariogenic challenge in situ. Dental cavities (4 mm wide, 4 mm long, and 1.5 mm deep) were prepared in 60 bovine teeth with enamel margins. Restorations were bonded with either adhesive Adper Single Bond 2 (3MESPE) or Optibond Solo Plus (Kerr). Forty restorations were included in an intra-oral palatal appliance that was used for 10 adult volunteers while the remaining 20 dental blocks were not submitted to any cariogenic challenge [NC group] and tested immediately. For the simulated cariogenic challenge [C+DA], each volunteer dropped 20% sucrose solution onto all blocks four times a day during 14 days and distilled water twice a day. In the inhibited cariogenic challenge group [C + FA], the same procedure was done, but slurry of fluoride dentifrice (1.100 ppm) was applied instead of water. The restored bovine blocks were sectioned to obtain a slice for cross-sectional Vickers microhardness evaluation and resin-dentin bonded sticks (0.8 mm(2)) for resin-dentin microtensile evaluation. Data were evaluated by two-way ANOVA and Tukey's tests (α = 0.05). Statistically lower microhardness values and degradation of the resin-dentin bonds were only found in the C + DW group for both adhesives. The in situ model seems to be a suitable short-term methodology to investigate the degradation of the resin-dentin bonds under a more realistic condition.
School of Dentistry, Department of Restorative Dentistry, University Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil.
Stella Maria Glaci
SM
Reinke
Juliana Aparecida de Campos
JA
Lawder
Sindianara
S
Divardin
Daniela
D
Raggio
Alessandra
A
Reis
Alessandro Dourado
AD
Loguercio
D000328:Adult; D000818:Animals; D002417:Cattle; D003731:Dental Caries; D003804:Dentin; D005260:Female; D006801:Humans; D008297:Male; D008422:Materials Testing; D008954:Models, Biological; D012117:Resins, Synthetic / Q000737:chemistry*; D013997:Time Factors
D012117:Resins, Synthetic