Microarray-guided discovery of two-photon (2P) small molecule probes for live-cell imaging of cysteinyl cathepsin activities.
A microarray immobilized with 105 aldehyde-containing small molecules was screened against mammalian cell lysates over-expressing cathepsin L to identify two potent inhibitors, which were subsequently converted into cell-permeable probes capable of live-cell imaging of endogenous cysteinyl cathepsin activities by two-photon fluorescence microscopy.
Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
Zhenkun
Z
Na
Lin
L
Li
Mahesh
M
Uttamchandani
Shao Q
SQ
Yao
D000447:Aldehydes / Q000737:chemistry; D056668:Cathepsin L / Q000037:antagonists & inhibitors / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D046228:Microarray Analysis; D036641:Microscopy, Fluorescence, Multiphoton; D015335:Molecular Probes / Q000737:chemistry*; D011480:Protease Inhibitors / Q000737:chemistry
D000447:Aldehydes; D015335:Molecular Probes; D011480:Protease Inhibitors; D056668:Cathepsin L