Industrial-scale preparation of akebia saponin D by a two-step macroporous resin column separation.
A simple and efficient procedure for the industrial preparation of akebia saponin D, one of the bioactive compounds commonly found in the well-known Chinese Medicinal herb Dipsaci Radix, was developed. First, HPD-722 was selected from among 10 kinds of macroporous absorption resins. Following this step, the purity of akebia saponin D was increased about 10 times from 6.27% to 59.41%. In order to achieve a higher purity, ADS-7 was chosen from among five kinds of macroporous absorption resins, and the purity of akebia saponin D was increased from 59.41% to 95.05%. The result indicated HPD-722 and ADS-7 were the most suitable resins to purify akebia saponin D from Dipsaci Radix. Under these conditions, large-scale preparation of akebia saponin D was carried out successfully. The preparation method is simple, efficient, and has been demonstrated to be effective for large scale preparations of akebia saponin D from Dipsaci Radix.
State Key Laboratory of Natural Products and Functions, China Pharmaceutical University, Ministry of Education, Nanjing, China. ywupub@gmail.com
Yue
Y
Wu
De
D
Ji
Yunfei
Y
Liu
Chunfeng
C
Zhang
Zhonglin
Z
Yang
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D049917:Dipsacaceae / Q000737:chemistry; D007221:Industry / Q000379:methods*; D007700:Kinetics; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*; D012503:Saponins / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification*; D012996:Solutions
D012117:Resins, Synthetic; D012503:Saponins; D012996:Solutions; C534004:akebia saponin D