Pediatric brainstem gangliogliomas show overexpression of neuropeptide prepronociceptin (PNOC) by microarray and immunohistochemistry.
BACKGROUND
Gangliogliomas (GGs) primary to brainstem are rare, with the overwhelming majority of GGs occurring in supratentorial, especially temporal lobe, locations. A less favorable prognosis exists for brainstem GGs, despite their usually identical WHO grade I status. Few large clinical series, and limited biological information, exists on these tumors, especially gene expression.


METHODS
Seven pediatric brainstem GGs, all with classic histological features, seen at our institution since 2000 were identified. Frozen section material was available for gene expression microarray profiling from five of seven brainstem GGs and compared with that from three non-brainstem pediatric GGs.


RESULTS
Significant upregulation of a number of genes was identified, most of which were involved in pathways of neural signaling, embryonic development, and pattern specification in pediatric brainstem GGs compared to non-brainstem. The single largest upregulated gene was a 256-fold increase in the expression of the neuropeptide prepronociceptin (PNOC); the protein product of this gene has been implicated in neuronal growth. Overexpression was validated by Western blot and by immunohistochemistry (IHC). Strong IHC expression of PNOC was seen in neoplastic neurons of 7/7 brainstem GGs, but was significantly weaker in non-brainstem GGs, and completely negative in normal pediatric autopsy brainstem controls.


CONCLUSIONS
PNOC IHC was often superior to IHC for NeuN, synaptophysin, or neurofilament for highlighting neoplastic neurons.
Department of Neuro-Oncology, Children's Hospital Colorado, Aurora, CO 80045, USA.
Michael H
MH
Chan
B K
BK
Kleinschmidt-Demasters
Andrew M
AM
Donson
Diane K
DK
Birks
Nicholas K
NK
Foreman
Sarah Z
SZ
Rush
D000293:Adolescent; D020295:Brain Stem Neoplasms / Q000235:genetics* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D018303:Ganglioglioma / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D046228:Microarray Analysis; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D011957:Receptors, Opioid / Q000235:genetics* / Q000378:metabolism
D011498:Protein Precursors; D011957:Receptors, Opioid; C121527:prepronociceptin