Development of Leptospirillum ferriphilum dominated consortium for ferric iron regeneration and metal bioleaching under extreme stresses.
Activated iron oxidizing consortium SR-BH-L enriched from Rajpardi lignite mine soil sample gave iron oxidation rate 1954 mg/L/h. Developed novel polystress resistant consortium oxidized ferrous iron under 11cP viscosity, 7.47 M ionic strength, 2.3 pH and g/L of 0.50 cadmium, 3.75 copper, 0.20 lead, 92.00 zinc, 6.4 sodium, 5.5 chloride, 154 sulphate and 393.8 TDS. The developed consortium showed 78.0% and 70.0% copper and zinc extraction from polymetallic bulk concentrate in monophasic bioleaching process. The bioregenerated ferric by the consortium in leachate showed 80.81% and 54.0% copper and zinc leaching in only 30 and 90 min. The DGGE analysis indicated the presence of 11 OTUs in the consortium. 16S rRNA gene sequence (JN797729) of the dominant band on DGGE shared >99% similarity with Leptospirillum ferriphilum. RE digestion analysis of the total 16S rRNA gene also illustrated the dominance of L. ferriphilum in the consortium.
Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad 380 009, Gujarat, India.
Bhargav C
BC
Patel
Devayani R
DR
Tipre
Shailesh R
SR
Dave
D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D003300:Copper / Q000302:isolation & purification; D058645:Denaturing Gradient Gel Electrophoresis; D004587:Electrophoresis, Agar Gel; D007501:Iron / Q000378:metabolism*; D059013:Microbial Consortia; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping; D013312:Stress, Physiological; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D015032:Zinc / Q000302:isolation & purification
D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D003300:Copper; D007501:Iron; D015032:Zinc