Comparison of ammonia-oxidizing bacterial community structure in membrane-assisted bioreactors using PCR-DGGE and FISH.
The ammonia-oxidizing bacterial (AOB) communities in three membrane bioreactors (MBRs) were monitored for 2 months after an acclimation period in order to investigate the influence of sludge age and medium type on AOB changeability and its connection with nitrification effectiveness. One MBR with a sludge age of 4 days was fed with a synthetic medium, whereas the other two with sludge ages of 8 and 32 days were fed with landfill leachate. The research revealed that landfill leachate can be effectively treated in an MBR with a higher sludge age for longer periods of time and that this improvement in performance was correlated with an increase in AOB biodiversity. Interestingly, the medium type has a stronger influence on AOB biocenosis formation than the sludge age.
The Silesian University of Technology, Environmental Biotechnology Department, Akademicka str. 2, 44-100 Gliwice, Poland. aleksandra.ziembinska@polsl.pl
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Ziembińska
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Ciesielski
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Gnida
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Zabczyńki
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J
Surmacz-Górska
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K
Miksch
D000641:Ammonia / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D058448:Biota; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008566:Membranes / Q000382:microbiology*; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction / Q000379:methods; D012722:Sewage / Q000382:microbiology; D013997:Time Factors
D012722:Sewage; D000641:Ammonia