Porphyrins from a metagenomic library of the marine sponge Discodermia calyx.
Marine sponges harbouring uncultured symbiotic bacteria are important sources of biologically active compounds. Since they would be interesting resources to explore unknown functional genes by means of a metagenomic approach, we constructed a metagenomic library of the Japanese marine sponge Discodermia calyx. The functional screening afforded the two clones producing porphyrins as red pigments. The isolation and structural elucidation of the red pigments revealed that the major red pigment was Zn-coproporphyrin III. The sequence data of the clones identified genes encoding glutamyl-tRNA reductase along with other ORFs related to porphyrin biosynthesis.
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Rui
R
He
Toshiyuki
T
Wakimoto
Yuya
Y
Takeshige
Yoko
Y
Egami
Hiromichi
H
Kenmoku
Takuya
T
Ito
Bochu
B
Wang
Yoshinori
Y
Asakawa
Ikuro
I
Abe
D000818:Animals; D056186:Metagenomics / Q000379:methods*; D010802:Phylogeny; D011161:Porifera / Q000235:genetics / Q000378:metabolism*; D011166:Porphyrins / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA
D011166:Porphyrins