Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (∙)NO.
In this study, the effects of L-arginine-nitric-oxide ((∙)NO)-producing pathway on protein content of ubiquitin, as an important component of ubiquitin-proteasome system for protein removal, were investigated. We showed that L-arginine markedly decreased ubiquitin protein content in interscapular brown adipose tissue, both in thermogenic inactive (at room temperature) and thermogenic active (on cold) states; while in L-NAME-treated groups this effect was abolished. This result suggests that nitric oxide ((∙)NO), besides well established roles, is involved in this aspect of structure remodeling, as well.
Department of Physiology, Institute for Biological Research Sinisa Stankovic, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
Milica
M
Vucetic
Vesna
V
Otasevic
Ana
A
Stancic
Aleksandra
A
Jankovic
Milica
M
Markelic
Igor
I
Golic
Ksenija
K
Velickovic
Biljana
B
Buzadzic
Aleksandra
A
Korac
Bato
B
Korac
D000064:Acclimatization / Q000502:physiology*; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D003080:Cold Temperature; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D025801:Ubiquitin / Q000096:biosynthesis*
D004791:Enzyme Inhibitors; D025801:Ubiquitin; D009569:Nitric Oxide; D019331:NG-Nitroarginine Methyl Ester