Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.
Bone marrow stromal cell cultures contain multipotent cells that may have therapeutic utility for tissue restoration; however, the identity of the cell that maintains this function remains poorly characterized. We have utilized a unique model of murine bone marrow stroma in combination with liquid chromatography mass spectrometry to compare the nuclear, cytoplasmic and membrane associated proteomes of multipotent (MSC) (CD105+) and non-multipotent (CD105-) stromal cells. Among the 25 most reliably identified proteins, 10 were verified by both real-time PCR and Western Blot to be highly enriched, in CD105+ cells and were members of distinct biological pathways and functional networks. Five of these proteins were also identified as potentially expressed in human MSC derived from both standard and serum free human stromal cultures. The quantitative amount of each protein identified in human stromal cells was only minimally affected by media conditions but varied highly between bone marrow donors. This study provides further evidence of heterogeneity among cultured bone marrow stromal cells and identifies potential candidate proteins that may prove useful for identifying and quantifying both murine and human MSC in vitro.
Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, Health Products and Food Branch, Health Canada, Ottawa, Canada. michael.rosu-myles@hc-sc.gc.ca
Michael
M
Rosu-Myles
Yi-Min
YM
She
Joel
J
Fair
Gauri
G
Muradia
Jelica
J
Mehic
Pablo
P
Menendez
Shiv S
SS
Prasad
Terry D
TD
Cyr
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D002454:Cell Differentiation; D002853:Chromatography, Liquid; D000071063:Endoglin; D006801:Humans; D051379:Mice; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000276:immunology; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface