[Analysis of an acellular pigskin based nerve scaffold].
A scaffold fabricated with lysine/nerve growth factor (NGF)/poly (lactic acid coglycolic acid) copolymer (PLGA) and acellular pigskin was evaluated in vitro as a potential artificial nerve scaffold. Properties of the scaffold such as microstructure, mechanical property, degradation behavior in PBS and water, Schwann cell adhesion property, and release of NGF were investigated. Results showed PLGA had permeated into the porous structure of acellular pigskin; its breaking strength was 8.308 MPa, breaking extensibility was 38.98%, elastic modulus was 97.27 MPa. The porosities of the scaffold ranged from 68.3% to 81.2% with densities from 0.62 g/cm3 to 0.68 g/cm3. At 4 weeks of degradation in vitro, maximum mass loss ratio was 43.3%. The release of NGF could still be detected on the 30th day, and its accumulative release rate was 38%. Lysine added into the scaffold neutralized the acidoid preventing degradation of PLGA to maintain a solution pH value. Schwann cells had grown across the scaffold after co-cultivation for 15 days. These in vitro properties of the pigskin based composite might indicate its potentiality to be an artificial nerve scaffold.
Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Chongqing 400715, China.
Bin
B
Liu
Jinxing
J
Ke
Shaoxi
S
Cai
Xiaokun
X
Li
Lu
L
Zhang
Wenqi
W
Chen
Yaoguang
Y
Zhang
D062088:Acellular Dermis; D000818:Animals; D001672:Biocompatible Materials; D048091:Guided Tissue Regeneration; D019344:Lactic Acid / Q000494:pharmacology*; D008239:Lysine / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000737:chemistry / Q000494:pharmacology*; D009416:Nerve Regeneration; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D009414:Nerve Growth Factors; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D008239:Lysine