Olivibacter jilunii sp. nov., isolated from DDT-contaminated soil.
Bacterial strain 14-2A(T), isolated from a long-term DDT-contaminated soil in China, was characterized by using a polyphasic approach to clarify its taxonomic position. Strain 14-2A(T) was found to be Gram-negative, aerobic, non-spore-forming, non-motile, non-flagellated and rod-shaped. The new isolate was able to grow at 4-42 °C, pH 6.0-9.0 and with 0-5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the family Sphingobacteriaceae. The 16S rRNA gene sequence of strain 14-2A(T) showed the highest similarity with Olivibacter oleidegradans TBF2/20.2(T) (99.4 %), followed by Pseudosphingobacterium domesticum DC-186(T) (93.8 %), Olivibacter ginsengisoli Gsoil 060(T) (93.6 %), Olivibacter terrae Jip13(T) (93.1 %), Olivibacter soli Gsoil 034(T) (92.8 %) and Olivibacter sitiensis AW-6(T) (89.6 %). The DNA-DNA hybridization value between strains 14-2A(T) and O. oleidegradans TBF2/20.2(T) was 34.45±2.11 %. Strain 14-2A(T) contained phosphatidylethanolamine, phosphatidylmonomethylethanolamine, aminophospholipid and phosphatidylinositol mannoside as the major polar lipids. The DNA G+C content was 41.2 mol%. MK-7 is the major isoprenoid quinone. Summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH are the major fatty acids. The phenotypic and chemotaxonomic data confirmed the affiliation of strain 14-2A(T) to the genus Olivibacter. On the basis of the phylogenetic and phenotypic characteristics, and chemotaxonomic data, strain 14-2A(T) is considered to represent a novel species of the genus Olivibacter, for which the name Olivibacter jilunii sp. nov. is proposed; the type strain is 14-2A(T) ( = KCTC 23098(T) = CCTCC AB 2010105(T)).
Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
Kai
K
Chen
The Key Laboratory for Microbial Resources of Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Shu-Kun
SK
Tang
College of Life Science, Huaibei Normal University, Huaibei 235000, PR China.|Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
Guang-Li
GL
Wang
College of Life Science, Henan Normal University, Xinxiang 453002, PR China.
Guo-Xing
GX
Nie
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science, Haikou 571101, PR China.
Qin-Fen
QF
Li
NJAU Bioengineering of Environment Co., Ltd, Nanjing 210095, PR China.
Ji-Dong
JD
Zhang
The Key Laboratory for Microbial Resources of Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Wen-Jun
WJ
Li
Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
Shun-Peng
SP
Li
D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002681:China; D003634:DDT; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D024482:Vitamin K 2; C062629:menaquinone 7; D003634:DDT