Temperature-dependent antibody kinetics as a tool in antibody lead selection.
Antibody-antigen interactions can principally be classified into three different temperature-dependent kinetic rate profiles. The affinity K               (D) can persist, decrease, or increase in the temperature gradient. Today, the impact of temperature-dependent antibody kinetics is recognized, especially as part of the development of best in class monoclonal antibodies. Here, a robust surface plasmon resonance-based protocol is presented, which describes a sensitive temperature-dependent kinetic measurement and evaluation method.
Roche Diagnostics GmbH, Penzberg, Germany. michael.schraeml@roche.com
Michael
M
Schräml
Leopold
L
von Proff
D000818:Animals; D000937:Antigen-Antibody Reactions; D006801:Humans; D007700:Kinetics; D020349:Surface Plasmon Resonance; D013696:Temperature