Metabolic profiling of lipids by supercritical fluid chromatography/mass spectrometry.
This review describes the usefulness of supercritical fluid chromatography (SFC) for the metabolic profiling of lipids. First, non-targeted lipid profiling by SFC/MS is described. The use of SFC/MS allows for high-throughput, exhaustive analysis of diverse lipids, and hence, this technique finds potential applications in lipidomics. Development of a polar lipid profiling method with trimethylsilyl (TMS) derivatization widens the scope of applicability of SFC/MS. SFC is a high-resolution technique that is suitable for non-targeted profiling aimed at the simultaneous analysis of many components. Next, targeted lipid profiling by SFC/MS is described. SFC is useful for the separation of lipids, such as carotenoids and triacylglycerols, which have numerous analogs with similar structures. In addition, SFC/MS shows the maximum efficiency for the target analysis of lipids in a biological sample that includes many matrices. Finally, a high-resolution, high-throughput analytical system based on SFC/MS is stated to be suitable for lipidomics because it is useful not only for the screening of lipid mixtures (as a fingerprint method) but also for the detailed profiling of individual components.
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. bamba@bio.eng.osaka-u.ac.jp
Takeshi
T
Bamba
Jae Won
JW
Lee
Atsuki
A
Matsubara
Eiichiro
E
Fukusaki
D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis / Q000097:blood / Q000737:chemistry; D055432:Metabolomics; D053719:Tandem Mass Spectrometry / Q000379:methods*
D008055:Lipids