[Effects of different hepatic inflow occlusion methods on liver regeneration following partial hepatectomy in rats].
OBJECTIVE
To explore the effects of different hepatic inflow occlusion methods on liver regeneration in rats after partial hepatectomy (PH).


METHODS
Male Wistar-Furth rats were randomly assigned to three groups: control group, underwent 68% hepatectomy alone; occlusion of portal triad (OPT) group, subjected to occlusion of portal triad under portal blood bypass; and occlusion of portal vein (OPV) group, subjected to occlusion of portal vein under portal blood bypass. Blood flow was occluded for 20, 30, and 40 minutes before 68% hepatectomy. According to the 7-day survival of each group, a same occlusion time T was set. Each group was divided into two subgroups (n = 8), in which animals were killed 3 and 7 days later. Liver regeneration was calculated as a percent of initial liver weight. Immunohistochemistry for proliferating cell nuclear antigen (PCNA) and Ki-67 was performed to quantify proliferating cells. In addition, functional liver volume represented by 99Tc(m)-GSA radioactivity was assessed.


RESULTS
The safe tolerance limit time was 30 minutes for OPT group and 40 minutes for OPV group. At 3 days after PH, no significant difference was observed in the regeneration rate of each group (P > 0.05). However, liver radioactive activity, PCNA labeling index, and Ki-67 index of OPV group was significantly higher than those of OPT group (P < 0.05); the latter were similar to those of control group (P > 0.05). At 7 days after PH, no significant difference was observed in all indexes among three groups (P > 0.05).


CONCLUSIONS
Compared with Pringle maneuver, preserving the hepatic artery flow during portal triad blood inflow occlusion can promote remnant liver regeneration early after PH.
Department of Hepatobiliary Surgery and Hepatobiliary Surgical Institute, Chinese PLA General Hospital, Beijing 100853, China.
Peng-Fei
PF
Wang
Chong-Hui
CH
Li
Ai-Qun
AQ
Zhang
Shou-Wang
SW
Cai
Jia-Hong
JH
Dong
D000818:Animals; D006498:Hepatectomy / Q000379:methods*; D008099:Liver / Q000098:blood supply / Q000601:surgery; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D011184:Postoperative Period; D051381:Rats; D017208:Rats, Wistar