Peripheral blood gene expression profile of atherosclerotic coronary artery disease in patients of different ethnicity in Malaysia.
The molecular basis of coronary artery disease (CAD) has been widely studied in the western world but there is no published work on the Malaysian population. This study looked at the global gene expression profiling of the peripheral blood of patients with CAD from the 3 main ethnic groups in Malaysia. Male subjects selected were based on angiographically confirmed CAD (≥50% stenosis) and normal control subjects (0% stenosis) with age range of 55.6±5.3 and 51.0±5.5 years, respectively. The global gene expression of 12 angiographically documented CAD patients and 11 matched control subjects were performed. The combined group samples identified 6 up regulated differential expression (DE) genes (GHRL, LTA, CBS, HP, ITGA2B, and OLR1) and 12 down regulated DE genes (IL18R1, ITGA2B, IL18RAP, HP, OLR1, SOD2 ITGB3, IL1B, MMP9, PLA2G7, UTS2, and CBS) to be involved in CAD at the fold change of 1.3 with fault discovery rate (FDR) of 1%. Three genes, MMP9, IL1B, and SOD2 were down regulated in all the 3 ethnic groups making them potential biomarker candidates for CAD across all three ethnicities. Further verification in a cohort study is needed.
Faculty of Medicine, Cyberjaya University College of Medical Sciences, 63000 Cyberjaya, Selangor, Malaysia. hafizngoo@cybermed.edu.my
Mohd Hafiz Ngoo
MH
Abdullah
Zulhabri
Z
Othman
Hamdan Mohd
HM
Noor
Siti Suri
SS
Arshad
Ahmad Khairuddin Mohd
AK
Yusof
Rahman
R
Jamal
Abdul Rashid Abdul
AR
Rahman
D015415:Biomarkers / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics*; D015536:Down-Regulation; D005006:Ethnicity; D015870:Gene Expression; D006801:Humans; D008296:Malaysia; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D012984:Software; D015854:Up-Regulation
D015415:Biomarkers