[Detection of antioxidant active compounds in mori ramulus by HPLC-MS-DPPH].
OBJECTIVE
To study major antioxidant active compounds in Mori Ramulus.


METHODS
The combination of on-line HPLC method and liquid chromatography-mass spectrometry was used to identify major antioxidant compounds and their content was determined by HPLC-DAD (detection wavelength of 320 nm).


RESULTS
Oxyresveratrol was proved to be the major antioxidant compound in Mori Ramulus ethanolic extracts. Using the HPLC-DAD quantitative determination, Mori Ramulus oxyresveratrol had a good linear range of 14-1 260 mg x L(-1) (r = 0.999 96). The average recovery was 98.2% with RSD of 1.2%. This method is simple, rapid, accurate and sensitive. There was rich oxyresveratrol in Mori Ramulus and the content was significantly different according to the mulberry varieties.


CONCLUSIONS
The on-line HPLC-MS-DPPH method is applicable for the determination of antioxidant compounds in Mori Ramulus.
The College of Animal Science, Zhejiang University, Hangzhou 310029, China.
Zuofa
Z
Zhang
Lian'gen
L
Shi
D000975:Antioxidants / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D031625:Morus / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D013997:Time Factors
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad