Robust quantification of microvascular transit times via linear dynamical systems using two-photon fluorescence microscopy data.
Vascular transit time is an important indicator of microcirculatory health. We present a second-order-plus-dead-time (SOPDT) model for robust estimation of kinetic parameters characterizing microvascular bolus passage using two-photon fluorescence microscopy (2PFM) in anesthetized rats receiving somatosensory stimulation. This methodology enables quantification of transit time, time-to-peak, overshoot, and rate of bolus passage through the microvascular network. The overall transit time during stimulation, of 2.2 ± 0.1 seconds, was shorter (P ~ 0.0008) than that at rest (2.7 ± 0.2 seconds). When compared with conventional γ-variate modeling, the SOPDT modeling yielded better quality of fit both at rest (P<0.0001) and on activation (P<0.001).
Imaging Research, Sunnybrook Research Institute, Toronto, Ontario, Canada.
Lakshminarayan V
LV
Chinta
Liis
L
Lindvere
Bojana
B
Stefanovic
D000465:Algorithms; D000758:Anesthesia; D000818:Animals; D001776:Blood Circulation Time; D002560:Cerebrovascular Circulation / Q000502:physiology; D016001:Confidence Intervals; D003627:Data Interpretation, Statistical; D007700:Kinetics; D016014:Linear Models; D008297:Male; D008833:Microcirculation / Q000502:physiology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley