Reduced systemic bicyclo-prostaglandin-E2 and cyclooxygenase-2 gene expression are associated with inefficient erythropoiesis and enhanced uptake of monocytic hemozoin in children with severe malarial anemia.
In holoendemic Plasmodium falciparum transmission areas, severe malaria primarily occurs in children aged <48 months and manifests as severe malarial anemia [SMA; hemoglobin (Hb) < 6.0 g/dL]. Induction of high levels of prostaglandin-E(2) (PGE(2)) through inducible cyclooxygenase-2 (COX-2) is an important host-defense mechanism against invading pathogens. We have previously shown that COX-2-derived PGE(2) levels are reduced in children residing in hyperendemic transmission regions with cerebral malaria and in those with mixed sequelae of anemia and hyperparasitemia. Our in vitro studies further demonstrated that reduced PGE(2) was due to downregulation of COX-2 gene products following phagocytosis of malarial pigment (hemozoin, PfHz). However, as COX-2-PGE(2) pathways and the impact of naturally acquired PfHz on erythropoietic responses have not been determined in children with SMA, plasma and urinary bicyclo-PGE(2)/creatinine and leukocytic COX-2 transcripts were determined in parasitized children (<36 months) stratified into SMA (n = 36) and non-SMA (Hb ≥ 6.0 g/dL; n = 38). Children with SMA had significantly reduced plasma (P = 0.001) and urinary (P < 0.001) bicyclo-PGE(2)/creatinine and COX-2 transcripts (P = 0.007). There was a significant positive association between Hb and both plasma (r = 0.363, P = 0.002) and urinary (r = 0.500, P = 0.001)] bicyclo-PGE(2)/creatinine. Furthermore, decreased systemic bicyclo-PGE(2)/creatinine was associated with inefficient erythropoiesis (i.e., reticulocyte production index; RPI < 2.0, P = 0.026). Additional analyses demonstrated that plasma (P = 0.031) and urinary (P = 0.070) bicyclo-PGE(2)/creatinine and COX-2 transcripts (P = 0.026) progressively declined with increasing concentrations of naturally acquired PfHz by monocytes. Results presented here support a model in which reduced COX-2-derived PGE(2), driven in part by naturally acquired PfHz by monocytes, promotes decreased erythropoietic responses in children with SMA.
Laboratories of Parasitic and Viral Diseases, Centre for Global Health Research, Kenya Medical Research Institute, University of New Mexico, Kisumu, Kenya.
Samuel B
SB
Anyona
Prakasha
P
Kempaiah
Evans
E
Raballah
Gregory C
GC
Davenport
Tom
T
Were
Stephen N
SN
Konah
John M
JM
Vulule
James B
JB
Hittner
Charity W
CW
Gichuki
John M
JM
Ong'echa
Douglas J
DJ
Perkins
D000740:Anemia / Q000097:blood / Q000469:parasitology / Q000652:urine; D002675:Child, Preschool; D003404:Creatinine / Q000097:blood / Q000652:urine; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D015232:Dinoprostone / Q000097:blood / Q000652:urine; D004920:Erythropoiesis; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006420:Hemeproteins / Q000378:metabolism*; D006801:Humans; D007223:Infant; D016778:Malaria, Falciparum / Q000097:blood / Q000652:urine; D008297:Male; D009000:Monocytes / Q000378:metabolism / Q000469:parasitology; D018512:Parasitemia; D010587:Phagocytosis; D012720:Severity of Illness Index
D006420:Hemeproteins; C020753:hemozoin; D003404:Creatinine; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D015232:Dinoprostone