Integrated phospholipidomics and transcriptomics analysis of Saccharomyces cerevisiae with enhanced tolerance to a mixture of acetic acid, furfural, and phenol.
A mixture of acetic acid, furfural, and phenol (AFP), three representative lignocellulose-derived inhibitors, significantly inhibited the growth and bioethanol production of Saccharomyces cerevisiae. In order to uncover the mechanisms behind the enhanced tolerance of an inhibitor-tolerant S. cerevisiae strain (T), we measured the plasma membrane properties, which significantly influence cellular adaptation to inhibitors, of T strain and its parental strain (P) with and without AFP treatment. We integrated data obtained from multi-statistics-assisted phospholipidomics and parallel transcriptomics by using LC-tandem MS and microarray analysis. With the AFP treatment, the transcriptional changes of fatty acid metabolic genes showed a strong correlation with the increase of fatty-acyl-chain length of phosphatidylcholine (PC) and phosphatidylinositol (PI). This suggests a possible compensatory mechanism to cope with the increase of plasma membrane permeability and fluidity in both strains. Moreover, the absence of phosphatidylserine (PS) and phosphatidylethanolamine (PE) species from the most variable phospholipid species group was a discriminative feature of the T strain. This resulted from the decrease of CHO1 and increase of CHO2 levels of the T strain upon AFP treatment. These novel findings reveal that the coordinated transcription and phospholipid composition changes contribute to the increased robustness of the T strain and highlight potential metabolic engineering targets for mutants with higher tolerance.
Key Laboratory of Systems Bioengineering, Ministry of Education; Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University , Tianjin, PR China.
Jie
J
Yang
Ming-Zhu
MZ
Ding
Bing-Zhi
BZ
Li
Z Lewis
ZL
Liu
Xin
X
Wang
Ying-Jin
YJ
Yuan
D019342:Acetic Acid / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005662:Furaldehyde / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D008031:Lignin / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D050296:Microbial Viability / Q000187:drug effects / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010539:Permeability; D019800:Phenol / Q000494:pharmacology*; D010743:Phospholipids / Q000096:biosynthesis / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects*
D010743:Phospholipids; D029701:Saccharomyces cerevisiae Proteins; C036909:lignocellulose; D019800:Phenol; D000431:Ethanol; D008031:Lignin; D005662:Furaldehyde; D019342:Acetic Acid