Increased tumor homing and tissue penetration of the filamentous plant viral nanoparticle Potato virus X.
Nanomaterials with elongated architectures have been shown to possess differential tumor homing properties compared to their spherical counterparts. Here, we investigate whether this phenomenon is mirrored by plant viral nanoparticles that are filamentous (Potato virus X) or spherical (Cowpea mosaic virus). Our studies demonstrate that Potato virus X (PVX) and Cowpea mosaic virus (CPMV) show distinct biodistribution profiles and differ in their tumor homing and penetration efficiency. Analogous to what is seen with inorganic nanomaterials, PVX shows enhanced tumor homing and tissue penetration. Human tumor xenografts exhibit higher uptake of PEGylated filamentous PVX compared to CPMV, particularly in the core of the tumor. This is supported by immunohistochemical analysis of the tumor sections, which indicates greater penetration and accumulation of PVX within the tumor tissues. The enhanced tumor homing and retention properties of PVX along with its higher payload carrying capacity make it a potentially superior platform for applications in cancer drug delivery and imaging applications.
Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106, United States.
Sourabh
S
Shukla
Amber L
AL
Ablack
Amy M
AM
Wen
Karin L
KL
Lee
John D
JD
Lewis
Nicole F
NF
Steinmetz
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D045744:Cell Line, Tumor; D002642:Chick Embryo; D017798:Comovirus / Q000378:metabolism*; D003952:Diagnostic Imaging / Q000379:methods; D016503:Drug Delivery Systems / Q000379:methods; D019073:HT29 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000008:administration & dosage*; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000821:virology*; D050130:Oncolytic Virotherapy / Q000379:methods; D010944:Plants / Q000821:virology; D011092:Polyethylene Glycols / Q000008:administration & dosage; D017863:Potexvirus / Q000378:metabolism*; D014018:Tissue Distribution
D000970:Antineoplastic Agents; D011092:Polyethylene Glycols