Increased detection of cutaneous leishmaniasis in Norway by use of polymerase chain reaction.
Cutaneous leishmaniasis (CL) is a parasitic infection and occurs in tropical and subtropical regions worldwide and in the region of the Mediterranean Sea. The diagnosis is based on the clinical appearance and biopsy findings that may be supplemented with polymerase chain reaction (PCR). In this study 20 cases were selected if (i) a histopathological diagnosis of granulomatous dermatitis was made, (ii) CL was taken into consideration or (iii) the diagnosis was CL. PCR analysis with primers specific for leishmania was performed on archived histological specimens and was positive in 6 of the 20 cases. In two cases both the clinical and histopathological diagnosis concurred with CL. In the remaining four cases a clinical diagnosis other than CL was made. In two of these cases the histopathology showed granulomatous dermatitis, and detection of parasites led to consideration of CL. In the last two cases leishmaniasis was not taken into consideration by clinicians or pathologists. Our study shows that CL may occur more often than anticipated in Norway, but clinicians do not consider the diagnosis as often as they should. Pathologists may also fail to diagnose or suggest CL especially when parasites are not visualized in the histopathological specimen.
Department of Pathology, Oslo University Hospital-Rikshospitalet, Norway. kblonski@web.de
Katharina M
KM
Blonski
Norbert
N
Blödorn-Schlicht
Thomas M
TM
Falk
Ragnar S
RS
Faye
Ole P F
OP
Clausen
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045303:Cytochromes b / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D007891:Leishmania / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D009664:Norway / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies; D055815:Young Adult
D016054:DNA, Protozoan; D045303:Cytochromes b