Effects of rs769217 and rs1001179 polymorphisms of catalase gene on blood catalase, carbohydrate and lipid biomarkers in diabetes mellitus.
Oxidative stress and deficiency of the enzyme catalase, which is the primary scavenger of the oxidant H(2)O(2), may contribute to diabetes. The current study examined two polymorphisms in the catalase gene, -262C>nT in the promoter and 111C>T in exon 9, and their effects on blood catalase activity as well as on concentrations of blood glucose, haemoglobin A1c, triglyceride, cholesterol, HDL, LDL, ApoA-I and ApoB. Subjects were type-1 and type-2 diabetics. We evaluated PCR-single strand conformational polymorphism for 111C>T and PCR-restriction fragment length polymorphism for - 262C>T. TT genotype frequency of 111C>T polymorphism was increased in type-1 diabetes. Type-2 diabetics with the CC or CT genotypes had decreased catalase and increased glucose, hemoglobinA1c and ApoB. Type-2 diabetics who have TT genotype in -262C>T may have elevated risk for diabetes complications; these patients had the lowest mean catalase and HDL, as well as the highest glucose, haemoglobin A1c, cholesterol and ApoB.
Biomedical Laboratory, Department of Medical Laboratory and Diagnostic Imaging, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Hungary. goth@dote.hu
László
L
Góth
Teréz
T
Nagy
Zsuzsanna
Z
Kósa
Zsolt
Z
Fejes
Harjit Pal
HP
Bhattoa
György
G
Paragh
Miklós
M
Káplár
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D050260:Carbohydrate Metabolism / Q000235:genetics; D002241:Carbohydrates / Q000097:blood; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood* / Q000235:genetics*; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000201:enzymology* / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology* / Q000235:genetics; D005260:Female; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D017382:Reactive Oxygen Species; D055815:Young Adult
D015415:Biomarkers; D002241:Carbohydrates; D008055:Lipids; D017382:Reactive Oxygen Species; D002374:Catalase