Electrochemical oxidation of cysteine at a film gold modified carbon fiber microelectrode its application in a flow-through voltammetric sensor.
A flow-electrolytical cell containing a strand of micro Au modified carbon fiber electrodes (CFE) has been designedand characterized for use in a voltammatric detector for detecting cysteine using high-performance liquid chromatography. Cysteine is more efficiently electrochemical oxidized on a Au /CFE than a bare gold and carbon fiber electrode. The possible reaction mechanism of the oxidation process is described from the relations to scan rate, peak potentials and currents. For the pulse mode, and measurements with suitable experimental parameters, a linear concentration from 0.5 to 5.0 mg·L(-1) was found. The limit of quantification for cysteine was below 60 ng·mL(-1).
Department of Medical Chemistry, Chia Nan University of Pharmacy and Science, Tainan, Taiwan. e201466.wang@msa.hinet.net
Lai-Hao
LH
Wang
Wen-Shiuan
WS
Huang
D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D003545:Cysteine / Q000032:analysis*; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry; D008839:Microelectrodes; D010084:Oxidation-Reduction
D000077482:Carbon Fiber; D002244:Carbon; D006046:Gold; D003545:Cysteine