High lipid content of irradiated human melanoma cells does not affect cytokine-matured dendritic cell function.
Gamma irradiation is one of the methods used to sterilize melanoma cells prior to coculturing them with monocyte-derived immature dendritic cells in order to develop antitumor vaccines. However, the changes taking place in tumor cells after irradiation and their interaction with dendritic cells have been scarcely analyzed. We demonstrate here for the first time that after irradiation a fraction of tumor cells present large lipid bodies, which mainly contain triglycerides that are several-fold increased as compared to viable cells as determined by staining with Oil Red O and BODIPY 493/503 and by biochemical analysis. Phosphatidyl-choline, phosphatidyl-ethanolamine and sphingomyelin are also increased in the lipid bodies of irradiated cells. Lipid bodies do not contain the melanoma-associated antigen MART-1. After coculturing immature dendritic cells with irradiated melanoma cells, tumor cells tend to form clumps to which dendritic cells adhere. Under such conditions, dendritic cells are unable to act as stimulating cells in a mixed leukocyte reaction. However, when a maturation cocktail composed of TNF-alpha, IL-6, IL-1beta and prostaglandin E2 is added to the coculture, the tumor cells clumps disaggregate, dendritic cells remain free in suspension and their ability to efficiently stimulate allogeneic lymphocytes is restored. These results help to understand the events following melanoma cell irradiation, shed light about interactions between irradiated cells and dendritic cells, and may help to develop optimized dendritic cell vaccines for cancer therapy.
Centro de Investigaciones Oncológicas-Fundación Cáncer, Instituto Alexander Fleming, Cramer 1180, 1426, Buenos Aires, Argentina. gabriela.pizzurro@gmail.com
Gabriela A
GA
Pizzurro
Florencia P
FP
Madorsky Rowdo
Luciana M
LM
Pujol-Lereis
Luis A
LA
Quesada-Allué
Andrea M
AM
Copati
María P
MP
Roberti
Jean-Luc
JL
Teillaud
Estrella M
EM
Levy
María M
MM
Barrio
José
J
Mordoh
D015153:Blotting, Western; D019496:Cancer Vaccines / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D018920:Coculture Techniques; D016207:Cytokines / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry; D005720:Gamma Rays; D006801:Humans; D008055:Lipids; D008545:Melanoma / Q000737:chemistry* / Q000276:immunology* / Q000473:pathology
D019496:Cancer Vaccines; D016207:Cytokines; D008055:Lipids