Bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions.
The aim of this work was to evaluate the activity of bromelain in pineapple plants (Ananas comosus var. Comosus), Pérola cultivar, produced in vitro in different culture conditions. This enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. In this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. The most significant levels of bromelain were observed in leaf tissue after 4 months of culture in vitro in medium with a filter paper bridge, followed by medium gelled by the agar. The results of this study, regarding the different structures of the pineapple (leaves and stems) in vitro showed that the activity of bromelain varied depending on the culture conditions, the time and structure of which was quantified, ensuring a viable strategy in the production of seedlings with high levels of bromelain in subsequent phases of micropropagation.
Federal University of Sergipe, Av. Marechal Rondon s/n, 49100-000 Aracaju, SE, Brazil.
Jaci Lima
JL
Vilanova Neta
Ana
A
da Silva Lédo
Aloisio André Bonfim
AA
Lima
José Carlos Curvelo
JC
Santana
Nadjma Souza
NS
Leite
Denise Santos
DS
Ruzene
Daniel Pereira
DP
Silva
Roberto Rodrigues
RR
de Souza
D031230:Ananas / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism; D001963:Bromelains / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods*; D057075:Enzyme Assays / Q000379:methods*; D018515:Plant Leaves / Q000201:enzymology; D018547:Plant Stems / Q000201:enzymology; D059748:Proteolysis
D003470:Culture Media; D001963:Bromelains