Large scale biosynthesis of ganglioside analogues by RERF-LC-AI cells cultured in HYPERFlask.
The efficient production of ganglioside analogues was accomplished using RERF-LC-AI cells cultured in HYPERFlask (High Yield PERformance Flask). Eight kinds of ganglioside analogues (GM3, GM2, sialylparagloboside, GD3, di-sialylated lacto-N-tetraose, and another three kinds of analogues with intricate structures) were synthesized by the saccharide primer method using lung squamous-cell carcinoma line RERF-LC-AI and 12-azidododecyl β-lactoside primer. The yield for each analogue obtained using HYPERFlask was higher than yields obtained from 100-mm dishes.
Japan Chemical Innovation and Inspection Institute, Tokyo, Japan.
Yumiko
Y
Shimura
Junya
J
Suzuki
Miho
M
Muraoka
Maria Carmelita Zulueta
MC
Kasuya
Koji
K
Matsuoka
Kenichi
K
Hatanaka
D001386:Azides / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002240:Carbohydrate Sequence; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D005732:Gangliosides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007785:Lactose / Q000031:analogs & derivatives / Q000378:metabolism; D008168:Lung / Q000166:cytology*; D008969:Molecular Sequence Data
C421632:12-azidododecyl lactoside; D001386:Azides; D005732:Gangliosides; D007785:Lactose