The temporal analysis of yeast exponential phase using shotgun proteomics as a fermentation monitoring technique.
System biology and bioprocess technology can be better understood using shotgun proteomics as a monitoring system during the fermentation. We demonstrated a shotgun proteomic method to monitor the temporal yeast proteome in early, middle and late exponential phases. Our study identified a total of 1389 proteins combining all 2D-LC-MS/MS runs. The temporal Saccharomyces cerevisiae proteome was enriched with proteolysis, radical detoxification, translation, one-carbon metabolism, glycolysis and TCA cycle. Heat shock proteins and proteins associated with oxidative stress response were found throughout the exponential phase. The most abundant proteins observed were translation elongation factors, ribosomal proteins, chaperones and glycolytic enzymes. The high abundance of the H-protein of the glycine decarboxylase complex (Gcv3p) indicated the availability of glycine in the environment. We observed differentially expressed proteins and the induced proteins at mid-exponential phase were involved in ribosome biogenesis, mitochondria DNA binding/replication and transcriptional activator. Induction of tryptophan synthase (Trp5p) indicated the abundance of tryptophan during the fermentation. As fermentation progressed toward late exponential phase, a decrease in cell proliferation was implied from the repression of ribosomal proteins, transcription coactivators, methionine aminopeptidase and translation-associated proteins.
Bioresource Engineering, McGill University, Ste.-Anne-de-Bellevue, Quebec, Canada H9X 3V9.
Eric L
EL
Huang
Valérie
V
Orsat
Manesh B
MB
Shah
Robert L
RL
Hettich
Nathan C
NC
VerBerkmoes
Mark G
MG
Lefsrud
D000595:Amino Acid Sequence; D002455:Cell Division / Q000502:physiology; D049109:Cell Proliferation; D016000:Cluster Analysis; D005285:Fermentation / Q000502:physiology*; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000502:physiology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000378:metabolism; D013997:Time Factors
D005656:Fungal Proteins; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins