[Genetic analysis of echovirus 30 isolated from acute meningitis/encephalitis syndrome cases in Linyi city of Shandong province, China].
OBJECTIVE
To investigate the pathogen spectrum constitution of HEV related to acute meningitis/encephalitis syndrome (AMES) and to analyze the genetic characterization of the echovirus 30 (Echo30) isolates.


METHODS
Cerebrospinal fluid, stool, and/or throat swab specimens from 101 AMES cases were collected for virus isolation with RD and HEp-2 cell lines in Linyi city from April to December, 2010. After typing by neutralization test, the entire VP1 gene of the isolates were amplified by RT-PCR and sequenced. Homologous comparison was carried out and phylogenetic analysis were performed.


RESULTS
HEVs were isolated from 47 specimens of 34 patients (4 from cerebrospinal fluid, 18 from throut swab and 25 from stool), with 11 Echo30 isolates identified by microneutralization assay and molecular typing method. In alignment of VP1 sequences with global Echo30 isolates, relative high nucleotide homologies (91.2% - 95.4%) with Taian, Zhangqiu and Jiangsu isolates and high divergences (17.9% -19.6%) with prototype strain were observed. Nucleotide divergences among Linyi isolates were 0 - 10.2%, and 3 lineages were revealed via phylogenetic analysis, reflecting 3 transmission chains of Echo30 co-circulated in Linyi city, 2010.


CONCLUSIONS
Echo30 was the predominant serotype of AMES cases in Linyi city, 2010. These isolates possessed considerable divergence with Echo30 from other countries.
Institute of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan 250012, China.
Jing
J
Yang
Xiao-Li
XL
Zhang
Ze-Xin
ZX
Tao
Hai-Yan
HY
Wang
Xiao-Juan
XJ
Lin
Sheng-Xiang
SX
Ji
Yao
Y
Liu
Yu-Jun
YJ
Zhang
Ai-Qiang
AQ
Xu
D000208:Acute Disease; D002648:Child; D002675:Child, Preschool; D002681:China; D018792:Encephalitis, Viral / Q000821:virology*; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D008587:Meningitis, Viral / Q000821:virology*; D010802:Phylogeny