Hydrodynamic chromatography.
Hydrodynamic chromatography (HDC) has experienced a resurgence in recent years for particle and polymer characterization, principally because of its coupling to a multiplicity of physical detection methods. When coupled to light scattering (both multiangle static and quasi-elastic), viscometric, and refractometric detectors, HDC can determine the molar mass, size, shape, and structure of colloidal analytes continuously and as a function of one another, all in a single analysis. In so doing, it exposes the analytes to less shear force (and, hence, less potential for flow-induced degradation) than in, for instance, size-exclusion chromatography. In this review, we discuss the fundamental chromatographic underpinnings of this technique in terms of retention, band broadening, and resolution, and we describe the power of multidetector HDC with examples from the recent literature.
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA.
André M
AM
Striegel
Amandaa K
AK
Brewer
D000818:Animals; D002850:Chromatography, Gel / Q000266:history / Q000295:instrumentation / Q000379:methods*; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D057446:Hydrodynamics; D010316:Particle Size; D011108:Polymers / Q000737:chemistry
D011108:Polymers