Biomarkers of oxidative stress in epidermis of Tunisian pemphigus foliaceus patients.
BACKGROUND
Reactive oxygen species play a key role in the development of many dermatological disorders.


OBJECTIVE
The purpose of this study is to examine the lipid peroxidation, protein oxidation and antioxidative profile in Tunisian pemphigus foliaceus (PF) patients.


METHODS
Malondialdehyde (MDA), conjugated dienes (CD), protein thiol levels, catalase (CAT) and superoxide dismutase (SOD) activities were evaluated in skin biopsies of 13 patients compared to biopsies of 7 healthy controls.


RESULTS
Oxidative stress was confirmed in these three types of patient biopsies as compared to controls. Thus, MDA, CD levels and catalase CAT and SOD activities were significantly increased in lesional, perilesional and normal biopsies of PF patients than in those of control subjects. Protein oxidative was confirmed by lower levels of protein thiols in lesional, perilesional and normal biopsies than in control's biopsies. Otherwise, in patients, a significant rise of these biomarkers was observed in lesional and perilesional biopsies compared with normal biopsies.


CONCLUSIONS
This study shows that oxidative stress could be involved in the pathogenesis of PF by the spread of skin lesions and/or by the increase in auto-antibodies' reactivity.
Laboratoire d'Immunologie, hôpital Habib Bourguiba, Université de Sfax, Tunisie Laboratoire d'analyse, valorisation et sécurité des aliments, groupe de recherche, Biotechnologie et pathologies, Institut Supérieur de Biotechnologie de Sfax, Université de Sfax, Tunisie Service de Dermatologie, hôpital Hédi Chaker, Sfax, Tunisie Service de chirugie Maxillo-fasciale, hôpital Habib Bourguiba, Sfax, Tunisie.
O
O
Abida
B
B
Gargouri
R
R
Ben Mansour
M
M
Mseddi-Djemal
A
A
Masmoudi
M
M
Ben Ayed
M
M
Abdelmoula
H
H
Turki
S
S
Lassoued
H
H
Masmoudi
D015415:Biomarkers / Q000378:metabolism*; D001706:Biopsy; D016022:Case-Control Studies; D002374:Catalase / Q000378:metabolism; D004817:Epidermis / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D010392:Pemphigus / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014416:Tunisia
D015415:Biomarkers; D013438:Sulfhydryl Compounds; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase