Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission.
Optogenetic silencing using light-driven ion fluxes permits rapid and effective inhibition of neural activity. Using rodent hippocampal neurons, we found that silencing activity with a chloride pump can increase the probability of synaptically evoked spiking after photoactivation; this did not occur with a proton pump. This effect can be accounted for by changes to the GABA(A) receptor reversal potential and demonstrates an important difference between silencing strategies.
Department of Pharmacology, Oxford University, Oxford, UK.
Joseph V
JV
Raimondo
Louise
L
Kay
Tommas J
TJ
Ellender
Colin J
CJ
Akerman
D000818:Animals; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D016623:Ion Pumps / Q000378:metabolism / Q000528:radiation effects; D009433:Neural Inhibition / Q000528:radiation effects*; D009474:Neurons / Q000528:radiation effects*; D017494:Proton Pumps / Q000378:metabolism / Q000528:radiation effects; D051381:Rats; D011963:Receptors, GABA-A / Q000378:metabolism / Q000528:radiation effects; D009435:Synaptic Transmission / Q000502:physiology / Q000528:radiation effects
D016623:Ion Pumps; D017494:Proton Pumps; D011963:Receptors, GABA-A