Effects of bisphenol A on the proliferation and cell cycle of HBL-100 cells.
Bisphenol A (BPA) is an environmental estrogen that exhibits non-genotoxic carcinogenicity, causing concern globally. The aim was to investigate the effects of BPA on the proliferation and cell cycle of human normal breast cells HBL-100. An improved E-Screen assay was used to study cell proliferation, and flow cytometry was used to study cell cycle phases. Western blot analysis was utilized to detect cell cycle proteins and estrogen receptor α (ERα) expression. The results showed that the highest cell proliferation rate induced by BPA was at 1.0 × 10(-6)mol/L. At 1.0 × 10(-10), 1.0 × 10(-8), and 1.0 × 10(-6)mol/L, BPA promoted more cells to enter into G2/M phase and caused an increase in the expression of cyclinD1 and CDK4. After adding ICI182780 into the system, the promoting effects of BPA on cell proliferation and cell cycle change decreased, but these promoting effects were still significantly greater compared with the solvent control (P<0.05). Regardless of ICI182780 exposure, BPA did not have significant effect on ERα expression. BPA has estrogen-like activity and can stimulate HBL-100 proliferation and cell division through the estrogen receptor pathway. BPA may have other pathways through which it can exert stimulating effects and exhibit non-genotoxic carcinogenicity.
Department of Toxicology, School of Public Health, Peking University, Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, China.
Shuang
S
Wu
Xuetao
X
Wei
Jianjun
J
Jiang
Lanqin
L
Shang
Weidong
W
Hao
D001559:Benzhydryl Compounds / Q000494:pharmacology*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D006801:Humans; D010636:Phenols / Q000494:pharmacology*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D018797:Cell Cycle Proteins; D047628:Estrogen Receptor alpha; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds