Discovery, application and protein engineering of Baeyer-Villiger monooxygenases for organic synthesis.
Baeyer-Villiger monooxygenases (BVMOs) are useful enzymes for organic synthesis as they enable the direct and highly regio- and stereoselective oxidation of ketones to esters or lactones simply with molecular oxygen. This contribution covers novel concepts such as searching in protein sequence databases using distinct motifs to discover new Baeyer-Villiger monooxygenases as well as high-throughput assays to facilitate protein engineering in order to improve BVMOs with respect to substrate range, enantioselectivity, thermostability and other properties. Recent examples for the application of BVMOs in synthetic organic synthesis illustrate the broad potential of these biocatalysts. Furthermore, methods to facilitate the more efficient use of BVMOs in organic synthesis by applying e.g. improved cofactor regeneration, substrate feed and in situ product removal or immobilization are covered in this perspective.
Institute of Biochemistry, Dept of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany.
Kathleen
K
Balke
Maria
M
Kadow
Hendrik
H
Mallin
Stefan
S
Sass
Uwe T
UT
Bornscheuer
D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D001711:Biotransformation; D060326:Chemistry Techniques, Synthetic / Q000379:methods; D004952:Esters / Q000737:chemistry / Q000378:metabolism; D005658:Fungi / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007659:Ketones / Q000737:chemistry / Q000378:metabolism*; D007783:Lactones / Q000737:chemistry / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D015202:Protein Engineering / Q000379:methods; D013237:Stereoisomerism
D004952:Esters; D007659:Ketones; D007783:Lactones; D006899:Mixed Function Oxygenases