Polyherbal preparation for anti-diabetic activity: a screening study.
OBJECTIVE
To screen the Polyherbal preparation for anti-diabetic activity in rats.


METHODS
The blood glucose lowering activity of the Polyherbal preparation-I (1:1:1 of Wheat germ oil, Coriandrum sativum and Aloe vera) was studied in normal rats after oral administration at doses of 1.0 and 2.0 ml/kg and Polyherbal preparation-I, II (Wheat germ oil, fresh juice of C. sativum and Aloe vera in the ratio of 2:2:1), and III (Wheat germ oil, fresh juice of C. sativum and Aloe vera in the ratio of 1:2:2) on alloxan-induced diabetic rats, after oral administration at doses of 1.0 and 2.0 ml/kg. Blood samples were collected from the tail vein method at 0, 0.5, 1, 2, 4, 8, 12, and 24 h in normal rats and in diabetic rats at 0, 1, 3, 7, 15, and 30 days. Blood plasma glucose was estimated by the GOD/POD (glucose oxidase and peroxidase) method. The data was compared statistically using the one-way ANOVA method followed by the Dunnett multiple component test. Statistical significance was set at P<0.05.


RESULTS
The Polyherbal preparation-I produced significant (P<0.05) reduction in the blood glucose level of normal rats and Polyherbal preparation-I, II, and III produced significant (P<0.01) reduction in the blood glucose level of diabetic rats during 30 days study and compared with that of control and Glibenclamide.


CONCLUSIONS
The Polyherbal preparation-I showed a significant glucose lowering effect in normal rats and Polyherbal preparation-I, II, and III in diabetic rats. This preparation is going to be promising anti-diabetic preparation for masses; however, it requires further extensive studies in human beings.
Department of Pharmaceutical Sciences, Bhagwant University, Sikar Road, Ajmer, India.
Noopur
N
Srivastava
Gaurav
G
Tiwari
Ruchi
R
Tiwari
D000496:Alloxan; D000504:Aloe; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D028526:Coriandrum; D003920:Diabetes Mellitus / Q000139:chemically induced / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D008517:Phytotherapy; D010938:Plant Oils / Q000008:administration & dosage; D028321:Plant Preparations / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010938:Plant Oils; D028321:Plant Preparations; C050055:wheat germ oil; D000496:Alloxan