Gene set enrichment analysis identifies LIF as a negative regulator of human Th2 cell differentiation.
In this study we show that IL-4 is crucial during reinforcement window of human Th2 differentiation for optimal Th2 development. We have also shown here that during this stage, IL-4 helps in cellular decision-making process of differentiation versus proliferation. We have combined computational and experimental methods to analyze Th2 transcription network to name novel players of the process of Th2 differentiation. Here we report that LIF through STAT3 negatively regulates Th2 differentiation. This approach can be generalized to analyze "omics" data to identify key regulatory modules.
Immunology Group International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067, India.
Ubaid
U
Ullah
Parul
P
Tripathi
Riitta
R
Lahesmaa
Kanury V S
KV
Rao
D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D053605:Leukemia Inhibitory Factor / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D018418:Th2 Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D050796:STAT3 Transcription Factor; D015847:Interleukin-4