Histone modifications and lamin A regulate chromatin protein dynamics in early embryonic stem cell differentiation.
Embryonic stem cells are characterized by unique epigenetic features including decondensed chromatin and hyperdynamic association of chromatin proteins with chromatin. Here we investigate the potential mechanisms that regulate chromatin plasticity in embryonic stem cells. Using epigenetic drugs and mutant embryonic stem cells lacking various chromatin proteins, we find that histone acetylation, G9a-mediated histone H3 lysine 9 (H3K9) methylation and lamin A expression, all affect chromatin protein dynamics. Histone acetylation controls, almost exclusively, euchromatin protein dynamics; lamin A expression regulates heterochromatin protein dynamics, and G9a regulates both euchromatin and heterochromatin protein dynamics. In contrast, we find that DNA methylation and nucleosome repeat length have little or no effect on chromatin-binding protein dynamics in embryonic stem cells. Altered chromatin dynamics associates with perturbed embryonic stem cell differentiation. Together, these data provide mechanistic insights into the epigenetic pathways that are responsible for chromatin plasticity in embryonic stem cells, and indicate that the genome's epigenetic state modulates chromatin plasticity and differentiation potential of embryonic stem cells.
Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra campus 91904, Israel.
Shai
S
Melcer
Hadas
H
Hezroni
Eyal
E
Rand
Malka
M
Nissim-Rafinia
Arthur
A
Skoultchi
Colin L
CL
Stewart
Michael
M
Bustin
Eran
E
Meshorer
D000107:Acetylation; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002843:Chromatin / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006657:Histones / Q000378:metabolism*; D034904:Lamin Type A / Q000378:metabolism*; D008745:Methylation; D051379:Mice
D002843:Chromatin; D006657:Histones; D034904:Lamin Type A