Panorama of DNA hairpin folding observed via diffusion-decelerated fluorescence correlation spectroscopy.
Based on a confocal microscopy platform, we extended the FCS time window by three orders of magnitude to the s timescale by attaching a polystyrene microsphere. We simultaneously monitored the relaxations of multiple intermediates involved in DNA hairpin folding, thus offering a much more detailed view of the kinetics of hairpin folding experimentally.
Beijing National Laboratory for Molecular Sciences and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. zhaoxs@pku.edu.cn and State Key Laboratory for Structural Chemistry of Unstable and Stable Species and Biodynamic Optical Imaging Centre, Peking University, Beijing 100871, China.
Yandong
Y
Yin
Beijing National Laboratory for Molecular Sciences and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. zhaoxs@pku.edu.cn and State Key Laboratory for Structural Chemistry of Unstable and Stable Species and Biodynamic Optical Imaging Centre, Peking University, Beijing 100871, China.
Peng
P
Wang
Beijing National Laboratory for Molecular Sciences and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. zhaoxs@pku.edu.cn and State Key Laboratory for Structural Chemistry of Unstable and Stable Species and Biodynamic Optical Imaging Centre, Peking University, Beijing 100871, China.
Xin Xing
XX
Yang
Beijing National Laboratory for Molecular Sciences and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. zhaoxs@pku.edu.cn and State Key Laboratory for Structural Chemistry of Unstable and Stable Species and Biodynamic Optical Imaging Centre, Peking University, Beijing 100871, China.
Xun
X
Li
Beijing National Laboratory for Molecular Sciences and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. zhaoxs@pku.edu.cn and Synthetic and Functional Biomolecules Centre, Peking University, Beijing 100871, China and Department of Chemistry, The University of Chicago, Chicago IL 60637, USA.
Chuan
C
He
Beijing National Laboratory for Molecular Sciences and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. zhaoxs@pku.edu.cn and State Key Laboratory for Structural Chemistry of Unstable and Stable Species and Biodynamic Optical Imaging Centre, Peking University, Beijing 100871, China.
Xin Sheng
XS
Zhao
D004277:DNA, Single-Stranded / Q000737:chemistry*; D004058:Diffusion; D005456:Fluorescent Dyes / Q000737:chemistry; D008863:Microspheres; D009690:Nucleic Acid Conformation; D011137:Polystyrenes / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D019809:Streptavidin
D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D011137:Polystyrenes; D012235:Rhodamines; C005358:tetramethylrhodamine; D019809:Streptavidin