DNA polymerase beta is involved in the protection against the cytotoxicity and genotoxicity of cigarette smoke.
Reactive oxygen species (ROS) and oxidative DNA damage have been implicated in the cigarette smoke-induced cytotoxicity and genotoxicity. DNA polymerase β (polβ), a key base excision repair (BER) enzyme in repairing oxidative DNA damage, may play a crucial role in fighting against the cytotoxicity and genotoxicity of cigarette smoke. In this study, we applied a novel approach to collect cigarette smoke extract (CSE) and investigated the cytotoxic and genotoxic effects of CSE by using the mouse embryo fibroblasts that express wild-type of polβ (polβ(+/+)), null of polβ (polβ(-/-)) and overexpression of polβ (polβ(oe)). Our results showed that polβ(-/-) cells treated with CSE exhibited a higher ROS level and more DNA single-strand breaks and chromosomal aberrations than that of polβ(+/+) and polβ(oe) cells. These data suggested that polβ mediated-BER may involve in repairing the CSE-induced DNA damage and protection against the cytotoxicity and genotoxicity of CSE.
Department of Environmental Health, Sichuan University, West China School of Public Health, Chengdu, People's Republic of China.
Jie
J
Cui
Department of Environmental Health, Sichuan University, West China School of Public Health, Chengdu, People's Republic of China.
Wei
W
Zhao
Department of Environmental Health, Sichuan University, West China School of Public Health, Chengdu, People's Republic of China.
Xin
X
Xu
Department of Environmental Health, Sichuan University, West China School of Public Health, Chengdu, People's Republic of China.
Mo
M
Yang
Department of Environmental Health, Sichuan University, West China School of Public Health, Chengdu, People's Republic of China.
Yaou
Y
Ren
Department of Environmental Health, Sichuan University, West China School of Public Health, Chengdu, People's Republic of China. Electronic address: zhangzunzhen@163.com.
Zunzhen
Z
Zhang
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D045424:Complex Mixtures / Q000633:toxicity*; D003603:Cytotoxins / Q000633:toxicity*; D004249:DNA Damage; D019951:DNA Polymerase beta / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012906:Smoke; D012907:Smoking / Q000009:adverse effects; D014026:Nicotiana
D045424:Complex Mixtures; D003603:Cytotoxins; D009153:Mutagens; D017382:Reactive Oxygen Species; D012906:Smoke; D019951:DNA Polymerase beta