Photocatalytic reactive oxygen species production and phototoxicity of titanium dioxide nanoparticles are dependent on the solar ultraviolet radiation spectrum.
Generation of reactive oxygen species (ROS) by titanium dioxide nanoparticles (nano-TiO(2)) and its consequent phototoxicity to Daphnia magna were measured under different solar ultraviolet (UV) spectra by applying a series of optical filters in a solar simulator. Removing UV-B (280-320 nm) from solar radiation had no significant impact on photocatalytic ROS production of nano-TiO(2), whereas removal of UV-A (320-400 nm) decreased ROS production remarkably. Removal of wavelengths below 400 nm resulted in negligible ROS production. A linear correlation between ROS production and D. magna immobilization suggests that photocatalytic ROS production may be a predictor of phototoxicity for nano-TiO(2). Intracellular ROS production within D. magna was consistent with the immobilization of the organism under different solar UV spectra, indicating that oxidative stress was involved in phototoxicity. The dependence of nano-TiO(2) phototoxicity on environmentally realistic variations in solar radiation suggests that risk assessment of these nanomaterials requires careful evaluation of exposure conditions in the environment.
Office of Research and Development, Mid-Continent Ecology Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Duluth, Minnesota, USA.
Hongbo
H
Ma
Amanda
A
Brennan
Stephen A
SA
Diamond
D000818:Animals; D002384:Catalysis; D003621:Daphnia; D004785:Environmental Pollutants / Q000633:toxicity*; D053758:Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress; D055668:Photochemical Processes; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012993:Solar Energy; D014025:Titanium / Q000633:toxicity*; D014466:Ultraviolet Rays
D004785:Environmental Pollutants; D017382:Reactive Oxygen Species; C009495:titanium dioxide; D014025:Titanium