Scanometric analysis of DNA microarrays using DNA intercalator-conjugated gold nanoparticles.
We introduce a scanometric detection method for the analysis of DNA microarrays using DNA intercalator-conjugated gold nanoparticles that can be analyzed with the naked eye or with an optical scanner after the enhancement of the AuNPs. Moreover, we successfully detected a hemagglutinin-subtyping DNA array using this method.
Bio Nanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Republic of Korea.
Hyunmin
H
Cho
Juyeon
J
Jung
Bong Hyun
BH
Chung
D000818:Animals; D003124:Colorimetry / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D003630:Daunorubicin / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006388:Hemagglutinins / Q000235:genetics; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification; D007364:Intercalating Agents / Q000737:chemistry*; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009976:Orthomyxoviridae Infections / Q000175:diagnosis; D013552:Swine
D004279:DNA, Viral; D006388:Hemagglutinins; D007364:Intercalating Agents; D006046:Gold; D004247:DNA; D003630:Daunorubicin