Poor harvest of peripheral blood stem cell in donors with microcytic red blood cells.
BACKGROUND
The outcome of peripheral blood stem cell (PBSC) harvest depends on mobilization and leukapheresis. Some poor harvests might not be directly related to poor mobilizations.


METHODS
We retrospectively analyzed the results of 793 consecutive healthy donors who underwent PBSC donation to evaluate the impact of low mean corpuscular volume (MCV) of red blood cells on the outcomes of hematopoietic stem cell mobilization and leukapheresis.


RESULTS
The circulating CD34+ cells in peripheral blood after five doses of granulocyte-colony-stimulating factor injection were similar in donors with low MCV and those with normal MCV (68.0×10(6) /L vs. 69.2×10(6) /L, p=0.38). The procedural settings were not different between the two groups. However, the apheresis outcome of donors with low MCV was significantly lower in total CD34+ cells, cell dose, apheresis yield, and collection efficiency than those with normal MCV (277.6×10(6) vs. 455.0×10(6) ; 4.9×10(6) /kg vs. 8.2×10(6) /kg; 16.9×10(6) /L vs. 27.3×10(6) /L; 0.285 vs. 0.388; all p<0.0001). Similar results were noticed in subgroup analysis using the severity of microcytosis and Mentzer index for the donors with MCV of less than 80fL. The collection efficiency was significantly correlated with the MCV (r=0.30, p<0.0001).


CONCLUSIONS
Low MCV was associated with poor apheresis outcomes in PBSC donors. This effect is not related to poor mobilization of CD34+ cells into the peripheral blood. Further studies to elucidate the detailed mechanism and develop strategy to avoid poor harvest are necessary.
Department of Hematology and Oncology and the Department of Pediatrics, Buddhist Tzu-Chi General Hospital, Taiwan.
Tso-Fu
TF
Wang
Shu-Huey
SH
Chen
Shang-Hsien
SH
Yang
Yu-Chieh
YC
Su
Sung-Chao
SC
Chu
Dian-Kun
DK
Li
D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism; D001782:Blood Donors; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology*; D004913:Erythrocytes, Abnormal / Q000187:drug effects; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007937:Leukapheresis; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation; D012189:Retrospective Studies
D018952:Antigens, CD34; D016178:Granulocyte-Macrophage Colony-Stimulating Factor