Temporally controlled targeted somatic mutagenesis in mouse eye pigment epithelium.
To generate temporally controlled site-specific somatic mutations in the mouse eye pigment epithelium, we generated a TRP1-Cre-ER(T2) transgenic mouse line that expresses the tamoxifen-dependent Cre-ER(T2) recombinase under the control of the tyrosinase-related protein 1 (TRP1) promoter. Cre-ER(T2) transcripts were readily detected in the retinal pigment epithelium (RPE), and tamoxifen treatment of adult TRP1-Cre-ER(T2) transgenic mice induced efficient excision of floxed DNA in patches of RPE cells, in numerous epithelial cells of the iris and ciliary body, and in very few cells of the neural retina. Importantly, no excision was detected in any cells in the absence of tamoxifen treatment. Thus, the TRP1-Cre-ER(T2) mouse line provides a powerful tool to study in vivo gene functions in the mouse eye pigment epithelium.
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR7104/INSERM U964, Collège de France, Université de Strasbourg, Illkirch Cedex, France.
Mikiro
M
Mori
Laetitia
L
Gargowitsch
Jean-Marc
JM
Bornert
Jean-Marie
JM
Garnier
Manuel
M
Mark
Pierre
P
Chambon
Daniel
D
Metzger
D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods; D019426:Integrases / Q000235:genetics; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D016296:Mutagenesis; D010088:Oxidoreductases / Q000235:genetics*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D047490:Retinoid X Receptor alpha / Q000235:genetics
D008562:Membrane Glycoproteins; D047490:Retinoid X Receptor alpha; D010088:Oxidoreductases; C079621:Tyrp1 protein, mouse; C045073:Cre recombinase; D019426:Integrases