Peroxisome targeting of lycopene pathway enzymes in Pichia pastoris.
Cellular targeting of biosynthetic pathway enzymes is an invaluable technique in metabolic engineering to modify metabolic fluxes towards metabolite of interest. Especially, recombinant carotenoid biosynthesis in yeasts should be balanced with a precursor pathway present in a specific cellular location because yeasts, being eukaryotes, have more defined intracellular location. Here, peroxisomal targeting of lycopene pathway enzymes, CrtE, CrtB, and CrtI, by fusing to peroxisomal targeting sequence 1 (PTS1) in Pichia pastoris X-33 is described.
Department of Molecular Science and Technology, Ajou University, Suwon, South Korea. pclee@ajou.ac.kr
Pyung Cheon
PC
Lee
D002338:Carotenoids / Q000096:biosynthesis*; D046508:Culture Techniques; D005656:Fungal Proteins / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D000077276:Lycopene; D060847:Metabolic Engineering / Q000379:methods*; D008856:Microscopy, Fluorescence; D020675:Peroxisomes / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D010843:Pichia / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014170:Transformation, Genetic
D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D002338:Carotenoids; D000077276:Lycopene