New role for the ibeA gene in H2O2 stress resistance of Escherichia coli.
ibeA is a virulence factor found in some extraintestinal pathogenic Escherichia coli (ExPEC) strains from the B2 phylogenetic group and particularly in newborn meningitic and avian pathogenic strains. It was shown to be involved in the invasion process of the newborn meningitic strain RS218. In a previous work, we showed that in the avian pathogenic E. coli (APEC) strain BEN2908, isolated from a colibacillosis case, ibeA was rather involved in adhesion to eukaryotic cells by modulating type 1 fimbria synthesis (M. A. Cortes et al., Infect. Immun. 76:4129-4136, 2008). In this study, we demonstrate a new role for ibeA in oxidative stress resistance. We showed that an ibeA mutant of E. coli BEN2908 was more sensitive than its wild-type counterpart to H(2)O(2) killing. This phenotype was also observed in a mutant deleted for the whole GimA genomic region carrying ibeA and might be linked to alterations in the expression of a subset of genes involved in the oxidative stress response. We also showed that RpoS expression was not altered by the ibeA deletion. Moreover, the transfer of an ibeA-expressing plasmid into an E. coli K-12 strain, expressing or not expressing type 1 fimbriae, rendered it more resistant to an H(2)O(2) challenge. Altogether, these results show that ibeA by itself is able to confer increased H(2)O(2) resistance to E. coli. This feature could partly explain the role played by ibeA in the virulence of pathogenic strains.
INRA, UMR 1282 Infectiologie et Santé Publique, Nouzilly, France.
Maud
M
Fléchard
Mélanie A M
MA
Cortes
Maryline
M
Répérant
Pierre
P
Germon
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000096:biosynthesis; D001483:Base Sequence; D015536:Down-Regulation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D018384:Oxidative Stress; D017384:Sequence Deletion; D012808:Sigma Factor / Q000096:biosynthesis
D001426:Bacterial Proteins; C096350:CusC protein, E coli; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D006861:Hydrogen Peroxide