The optimization of sulfation modification conditions for ophiopogonpolysaccharide based on antiviral activity.
Ophiopogonpolysaccharide (OPS) was extracted by water decoction and ethanol precipitation, purified through eliminating protein by trichloroacetic acid method and column chromatography of DEAE-Cellulose-52, then sulfatedly modified by chlorosulfonic acid-pyridine method according to three-factors, ratio of chlorosulfonic acid to pyridine, reaction temperature and reaction time, and three level L₉(3⁴) orthogonal designed to obtain nine sulfated OPSs, sOPS₁-sOPS₉. Their effects on NDV to infect chick embryo fibroblast were compared by MTT assay taking the non-modified OPS as control. The results showed that sulfation modification could significantly enhance the antiviral activity of OPS, sOPS₃ presented best effect and the optimal modification conditions were the ratio of chlorosulfonic acid to pyridine of 1:4, the reaction temperature of 60 °C and the reaction time of 2 h.
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Jing
J
Zhang
Yuanliang
Y
Hu
Deyun
D
Wang
Tao
T
Qin
Cui
C
Liu
Xu
X
Liu
Xin
X
Sheng
Shanshan
S
Chang
Yunpeng
Y
Fan
Liwei
L
Guo
The Luong
TL
Nguyen
D000998:Antiviral Agents / Q000737:chemistry* / Q000494:pharmacology*; D009522:Newcastle disease virus / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000737:chemistry*
D000998:Antiviral Agents; D011134:Polysaccharides; D013431:Sulfates