Brumimicrobium mesophilum sp. nov., isolated from a tidal flat sediment, and emended descriptions of the genus Brumimicrobium and Brumimicrobium glaciale.
A Gram-stain-negative, aerobic, rod-shaped, non-motile and orange-coloured marine bacterium, YH207(T), was isolated from a tidal flat at Yeongheung-do on the coast of the Yellow Sea, Korea. 16S rRNA gene sequence analysis revealed that strain YH207(T) was affiliated with the family Cryomorphaceae and showed highest similarity to Brumimicrobium glaciale IC156(T) (95.4 %). Growth was observed at 11-36 °C, at pH 6.5-10.0 and with 0.4-7.0 % NaCl. The predominant cellular fatty acids when grown at 20 °C were iso-C15 : 0 (44.2 %), iso-C15 : 1 G (34.3 %), iso-C17 : 0 3-OH (8.7 %) and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c; 2.3 %). The major respiratory quinone was MK-6. Phosphatidylethanolamine, phosphatidylglycerol, three unidentified lipids, three unidentified aminophospholipids, one unidentified phospholipid, four unidentified aminolipids and three unidentified glycolipids were identified as major polar lipids. The DNA G+C content was 34.3 mol%. On the basis of the data from our polyphasic taxonomic study, strain YH207(T) should be classified in a novel species in the genus Brumimicrobium, for which the name Brumimicrobium mesophilum sp. nov. is proposed. The type strain is YH207(T) ( = KCCM 42331(T)  = JCM 14063(T)). Emended descriptions of the genus Brumimicrobium and Brumimicrobium glaciale Bowman et al. 2003 are also given.
Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
Sung-Hyun
SH
Yang
Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
Hyun-Seok
HS
Seo
Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
Hyun-Myung
HM
Oh
Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
Sang-Jin
SJ
Kim
Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
Jung-Hyun
JH
Lee
Marine Biotechnology Research Division, Korea Institute of Ocean Science & Technology, PO Box 29, Ansan 425-600, Republic of Korea.
Kae Kyoung
KK
Kwon
D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6