Effect of platelet-rich plasma with fibrin matrix on healing of intrasynovial flexor tendons.
OBJECTIVE
To investigate the effects of platelet-rich plasma (PRP) with fibrin matrix on the healing of intrasynovial flexor tendons in a rabbit model in vivo.


METHODS
We transected and repaired 156 toe flexors of 73 rabbits using the technique of Tsuge et al and a simple running epitendinous suture. We randomly assigned Repaired tendons to groups that recieved no additional treatment (control) or to which we applied PRP, fibrin (F), or PRP with fibrin matrix (PRP-F) at the repair site. We scored edema and adhesion at 2, 3, and 6 weeks after surgery, and linearly tested repaired tendons for load to failure. We also histologically evaluated tendons at 2 and 3 weeks.


RESULTS
Edema scores and adhesion scores did not significantly differ among the 4 groups at any time point. Mean load to failure in the PRP-F group (14.7 N) was the highest among the 4 groups at 2 weeks after surgery, and was significantly higher than in the control group (10.0 N). Median histological scores in the PRP-F group (3.3 points) were significantly higher than in the control group (1.0 point). Mean load to failure in the PRP-F group (16.1 N) was highest, and median histological scores in the PRP-F group (3.5 points) were higher than in the control group (2.4 points) at 3 weeks, although there were no significant differences at 3 or 6 weeks.


CONCLUSIONS
In a rabbit model of cut flexor tendons, PRP with fibrin matrix significantly increased healing strength within 2 weeks after surgery. Side effects such as increases in toe edema or adhesions around the tendons did not arise.


CONCLUSIONS
Platelet-rich plasma with fibrin matrix might help reduce the risk of repeated rupture after flexor tendon surgery, and lead to early rehabilitation.
Department of Orthopaedic Surgery, Yamagata University School of Medicine, Yamagata, Japan. d-satou@med.id.yamagata-u.ac.jp
Daisuke
D
Sato
Masatoshi
M
Takahara
Atsushi
A
Narita
Junichi
J
Yamakawa
Junichi
J
Hashimoto
Hitoshi
H
Ishikawa
Toshihiko
T
Ogino
D000704:Analysis of Variance; D000818:Animals; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D053657:Platelet-Rich Plasma; D011817:Rabbits; D011897:Random Allocation; D018709:Statistics, Nonparametric; D013314:Stress, Mechanical; D013536:Suture Techniques; D013710:Tendons / Q000378:metabolism* / Q000601:surgery*; D014945:Wound Healing
D015718:Fibrin Tissue Adhesive