Effect of acellular dermal matrix as a delivery carrier of adipose-derived mesenchymal stem cells on bone regeneration.
The purpose of this study is to evaluate the effect of acellular dermal matrix (ADM) as a delivery carrier of adipose-derived mesenchymal stem cells (ASCs) on bone regeneration in athymic murine calvarial bone defect. Paired-critical size defects in nude rat skull were made. The right-side defects received ASCs/ADM or only ADM, whereas the left-side defect was not treated. In 3D images, new bone formation in the ASCs/ADM group was apparent at 4 wk, but in the ADM group at 8 wk. At 4 and 8 wk, bone mineral density and tissue volume in rats that received ASCs/ADM were significantly greater than rats that received ADM and control groups. Histological examination revealed that the defect was repaired by bone in the ASCs/ADM group, whereas only minimal bone island with fibrous connection was observed in the control group. In histomorphometric analysis, the total healing score in the ASCs/ADM group at 4 wk was significantly higher than the ADM and negative control group, whereas the score of 8 wk was similar between the ASCs/ADM and ADM group. ASCs/ADM implants promote new bone formation more rapidly than ADM only or no treatment. ADM seeded with ASCs may be potentially useful as a future biomaterial option in bone implants.
Department of Veterinary Surgery, Seoul National University, College of Veterinary Medicine, Seoul, Korea.
Hee Jung
HJ
Kim
Sung Su
SS
Park
Sun Young
SY
Oh
Hyoju
H
Kim
Oh-Kyeong
OK
Kweon
Heung Myong
HM
Woo
Wan Hee
WH
Kim
D000273:Adipose Tissue; D000818:Animals; D001861:Bone Regeneration; D020405:Dermis; D004285:Dogs; D005109:Extracellular Matrix; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells; D051379:Mice; D051381:Rats; D011923:Rats, Nude; D013997:Time Factors; D014183:Transplantation, Heterologous