Selective packaging of cellular miRNAs in HIV-1 particles.
Retroviral particles are known to package specific host cell components such as RNA molecules in addition to the two copies of the viral RNA genome. The highly sensitive SOLiD sequencing technology was used to determine the cellular miRNA content of human immunodeficiency virus type 1 (HIV-1) particles. We determined the relative concentration of cellular miRNAs in a T cell line and several primary cell subsets before and after HIV-1 infection, and compared those values to the miRNA content of virion particles. A small subset of the cellular miRNAs is dramatically concentrated in the virions up to 115 fold, suggesting a biological function in HIV-1 replication.
Laboratory of Experimental Virology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
Nick C T
NC
Schopman
Thijs
T
van Montfort
Marcel
M
Willemsen
Stefanie A
SA
Knoepfel
Georgios
G
Pollakis
Antoine
A
van Kampen
Rogier W
RW
Sanders
Joost
J
Haasnoot
Ben
B
Berkhout
D002478:Cells, Cultured; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D017421:Sequence Analysis; D013601:T-Lymphocytes / Q000821:virology; D014771:Virion / Q000737:chemistry*; D019065:Virus Assembly
D035683:MicroRNAs