Molecular characterization and panton-valentine leucocidin typing of community-acquired methicillin-sensitive Staphylococcus aureus clinical isolates.
Limited comprehensive molecular typing data exist currently for Panton-Valentine leucocidin (PVL)-positive, methicillin-sensitive Staphylococcus aureus (PVL-MSSA) clinical isolates. Characterization of PVL-MSSA isolates by multilocus sequence typing (MLST) and spa typing in this study showed a genetic similarity to PVL-positive, methicillin-resistant S. aureus (PVL-MRSA) strains, although three novel spa types and a novel MLST (ST1518) were detected. Furthermore, the detection of PVL phages and haplotypes in PVL-MSSA identical to those previously found in PVL-MRSA isolates highlights the role these strains may play as precursors of emerging lineages of clinical significance.
Centre for Healthcare Associated Infections, School of Molecular Medical Sciences, University of Nottingham, Centre for Biomolecular Sciences, University Park, Nottingham, United Kingdom. mrxko2@nottingham.ac.uk
Kome
K
Otokunefor
Tim
T
Sloan
Angela M
AM
Kearns
Richard
R
James
D000293:Adolescent; D000328:Adult; D000368:Aged; D001427:Bacterial Toxins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000382:microbiology*; D005098:Exotoxins / Q000235:genetics*; D005260:Female; D006239:Haplotypes; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007956:Leukocidins / Q000235:genetics*; D008297:Male; D008712:Methicillin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D017720:Molecular Epidemiology; D058889:Molecular Typing; D039002:Prophages / Q000235:genetics; D013203:Staphylococcal Infections / Q000382:microbiology*; D013204:Staphylococcus Phages / Q000235:genetics; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics*; D055815:Young Adult
D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D037521:Virulence Factors; D008712:Methicillin