A photochromic ATP analogue driving a motor protein with reversible light-controlled motility: controlling velocity and binding manner of a kinesin-microtubule system in an in vitro motility assay.
We synthesized two photochromic ATP analogues (ATP-Azos) featuring azobenzene derivatives tethered at the 2' position of the ribose ring. In the presence of the ATP-Azo tethering p-tert-butylazobenzene, we observed reversible photo-control of the motility, velocity and binding manner, of a kinesin-microtubule system in an in vitro motility assay.
Research Institute for Electronic Science, Hokkaido University, N20, W10, Kita-ku, Sapporo, Hokkaido, Japan.
Takashi
T
Kamei
Tuyoshi
T
Fukaminato
Nobuyuki
N
Tamaoki
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism*; D001391:Azo Compounds / Q000737:chemistry / Q000378:metabolism; D016547:Kinesins / Q000378:metabolism* / Q000648:ultrastructure; D008027:Light; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D014466:Ultraviolet Rays
D001391:Azo Compounds; D000255:Adenosine Triphosphate; D016547:Kinesins; C009850:azobenzene