Development of an integrated chip for automatic tracking and positioning manipulation for single cell lysis.
This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.
Department of Biomechatronic Engineering, National Chiayi University, Chiayi 600, Taiwan. youngcw@mail.ncyu.edu.tw
Chao-Wang
CW
Young
Jia-Ling
JL
Hsieh
Chyung
C
Ay
D045744:Cell Line, Tumor; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D004566:Electrodes; D001330:Electronic Data Processing; D018274:Electroporation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D046210:Microfluidic Analytical Techniques; D009997:Osmotic Pressure
D004247:DNA