[Study on vector-specific and foreign gene-specific immune responses induced by rAd5 and rAAV2/1 vaccines].
OBJECTIVE
To compare the foreign gene-specific and vector-specific immune responses in BALB/c mice immunized with rAd5 or rAAV2/1 expressing the same gene.


METHODS
BALB/c mice were immunized with rAd5-gag or rAAV2/1-gag once, HIV-1 Gag-specific and vector-specific cellular immune responses were analyzed by Elispot assay, HIV-1 P24-specific IgG and vector-specific IgG were tested by ELISA assay.


RESULTS
Mice immunized with rAd5-gag induced potent Gag-specific cellular immune responses and that were significantly higher than Ad5-specfic cellular responses, while rAAV2/1-gag elicited weak Gag-specific and AAV2/1-specific cellular responses. Both P24-specific and Ad5-specific IgG titers induced by rAd5-gag were high and in similar level. Higher level of P24-specific IgG was found in mice inoculated with rAAV2/1-gag than rAd5-gag. And the P24-specific IgG titers were higher than the vector-specific IgG titers in mice immunized with rAAV2/1.


CONCLUSIONS
rAd5 could elicit strong foreign gene-specific cellular and humoral immune responses, weak vector-specific cellular responses and strong vector-specific antibodies, rAAV2/1 could induce potent foreign gene-specific antibodies that were much higher than vector-specific IgG, while both foreign gene-specific and vector-specific cellular responses were very low.
Laboratory of Virology and Pharmocology, College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100052, China.
Liu
L
Yang
Yun
Y
Li
Ling
L
Yang
Ling-Fei
LF
Zhang
Xia
X
Feng
Shuang-Qing
SQ
Yu
Dan-Ying
DY
Chen
Ze-Lin
ZL
Li
Yi
Y
Zeng
D016915:AIDS Vaccines / Q000276:immunology*; D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000229:Dependovirus / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors / Q000276:immunology*; D016655:HIV Core Protein p24 / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D014614:Vaccines, Synthetic / Q000276:immunology
D016915:AIDS Vaccines; D000914:Antibodies, Viral; D016655:HIV Core Protein p24; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic