Expression, purification and functional reconstitution of slack sodium-activated potassium channels.
The slack (slo2.2) gene codes for a potassium-channel α-subunit of the 6TM voltage-gated channel family. Expression of slack results in Na(+)-activated potassium channel activity in various cell types. We describe the purification and reconstitution of Slack protein and show that the Slack α-subunit alone is sufficient for potassium channel activity activated by sodium ions as assayed in planar bilayer membranes and in membrane vesicles.
Department of Cellular and Molecular Physiology, Yale University, New Haven, CT 06520-8026, USA.
Yangyang
Y
Yan
Youshan
Y
Yang
Shumin
S
Bian
Fred J
FJ
Sigworth
D002460:Cell Line; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D008094:Lithium / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D055550:Protein Stability; D021122:Protein Subunits / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
D015221:Potassium Channels; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D008094:Lithium; D012964:Sodium