Polarization-resolved second harmonic generation microscopy with a four-channel Stokes-polarimeter.
We developed a four-channel photon counting based Stokes-polarimeter for spatial characterization of polarization effects in second harmonic generation (SHG). We have implemented a calibration technique allowing quantitative measurement of polarization parameters, such as the degree of polarization (DOP), degree of linear polarization (DOLP), degree of circular polarization (DOCP), as well as anisotropy from the acquired Stokes parameters. The technique is used as contrast mechanism to characterize the polarization properties from two potassium dihydrogen phosphate (KDP) micro-crystals and collagen type-I in SHG microscopy.
Institute of Biophotonics, National Yang-Ming University, 155, Li-Nong St. Taipei 11221, Taiwan.
Nirmal
N
Mazumder
Jianjun
J
Qiu
Matthew R
MR
Foreman
Carlos Macías
CM
Romero
Chih-Wei
CW
Hu
Han-Ruei
HR
Tsai
Peter
P
Török
Fu-Jen
FJ
Kao
D004867:Equipment Design; D019544:Equipment Failure Analysis; D018613:Microscopy, Confocal / Q000295:instrumentation*; D015203:Reproducibility of Results; D057109:Scanning Laser Polarimetry / Q000295:instrumentation*; D012680:Sensitivity and Specificity