Bacterial colonization and beta defensins in the female genital tract in HIV infection.
Beta defensins are antimicrobial peptides that serve to protect the host from microbial invasion at skin and mucosal surfaces. Here we explore the relationships among beta defensin levels, total bacterial colonization, and colonization by bacterial vaginosis (BV)-related bacteria and lactobacilli in the female genital tract in HIV infected women and healthy controls. Cervicovaginal lavage (CVL) samples were obtained from 30 HIV-infected women and 36 uninfected controls. Quantitative PCR assays were used to measure DNA levels of bacterial 16S ribosomal DNA (reflective of total bacterial load), and levels of three BV-related bacteria, three Lactobacillus species (L. crispatus, L. iners and L. jensenii), and total Lactobacillus levels in CVL. Levels of human beta defensins (hBD-2 and hBD-3) were quantified by ELISA. In viremic HIV+ donors, we found that CVL levels of bacterial 16S rDNA were significantly increased, and inversely correlated with peripheral CD4+ T cell counts in HIV+ women, and inversely correlated with age in both HIV+ women and controls. Although CVL DNA levels of BV-associated bacteria tended to be increased, and CVL levels of Lactobacillus DNAs tended to be decreased in HIV+ donors, none of these differences was significant. CVL levels of hBD-2 and hBD-3 were correlated and were not different in HIV+ women and controls. However, significant positive correlations between hBD-3 levels and total bacterial DNA levels in controls were not demonstrable in HIV+ women; the significant positive correlations of hBD2 or hBD-3 and three Lactobacillus species in controls were also not demonstrable in HIV+ women. These results suggest that HIV infection is associated with impaired regulation of innate defenses at mucosal sites.
Division of Infectious Diseases, Department of Medicine, Center for AIDS Research, Case Western Reserve University and University Hospitals/Case Medical Center, 2109 Adelbert Rd, CWRU BRB1048B, 4984, Cleveland, OH 44106, USA. jxw103@case.edu
Wei
W
Jiang
Santosh K
SK
Ghosh
Rebecca
R
Flyckt
Magdalena
M
Kalinowska
David
D
Starks
Richard
R
Jurevic
Aaron
A
Weinberg
Michael M
MM
Lederman
Benigno
B
Rodriguez
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000382:microbiology*; D000328:Adult; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014621:Vagina / Q000276:immunology / Q000382:microbiology*; D044364:Vaginal Douching; D014626:Vaginal Smears; D016585:Vaginosis, Bacterial / Q000276:immunology / Q000382:microbiology*; D019562:Viral Load; D023083:beta-Defensins / Q000276:immunology*
C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; D004269:DNA, Bacterial; D023083:beta-Defensins