Structural characterization and antitumor and mitogenic activity of a lectin from the gill of bighead carp (Aristichthys nobilis).
In this study, we investigated the gross structure, secondary structure, and antitumor and mitogenic activity of GANL, a lectin from the gill of bighead carp (Aristichthys nobilis). We used infrared spectroscopy, β-elimination, and circular dichroism spectroscopy to determine the structure of GANL. We measured antiproliferation activity against six human tumor cell lines and mitogenic activity against murine splenocytes using the MTT assay. Based on infrared spectroscopy and β-elimination, we conclude that GANL is a glycoprotein. The protein and carbohydrate moieties are joined by O-glycosidic linkage. A circular dichroism spectroscopic analysis revealed that the secondary structure of GANL consists of α-helices (34.8 %), β-sheets (12.1 %), β-turns (24.5 %), and unordered structures (33.0 %). GANL exerted potent antitumor activity against the HeLa cell line (IC(50) = 11.86 μg/mL) and a mitogenic effect on murine splenocytes in the MTT assay. GANL, a lectin that is isolated from the gills of bighead carp, is a glycoprotein with potent antitumor and mitogenic activity.
School of Marine Science and Technology, Huaihai Institute of Technology, 59 Changwu Road, Lianyungang, 222005, China.
Dongrui
D
Yao
School of Marine Science and Technology, Huaihai Institute of Technology, 59 Changwu Road, Lianyungang, 222005, China. pskgx@163.com.
Saikun
S
Pan
School of Marine Science and Technology, Huaihai Institute of Technology, 59 Changwu Road, Lianyungang, 222005, China.
Mingqian
M
Zhou
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002347:Carps / Q000378:metabolism*; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D005260:Female; D005880:Gills / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology
D000970:Antineoplastic Agents; D037102:Lectins; D008934:Mitogens