[Acute and chronic stress induced changes in gene transcriptions related to Alzheimer's disease].
Preclinical and clinical studies demonstrate that stress may be implicated in the risk of neurodegenerative diseases such as Alzheimer's disease (AD). Our study aimed to investigate the effects of acute and chronic immobilization stress (IS) on the gene transcriptions of beta-actin, amyloid precursor protein (APP) and mitogen activated protein kinase-1 (MAPK-1), proteins related to synaptic plasticity and neuronal degeneration. Male Wistar rats were exposed to IS for five hours daily for 3 days (acute stress) or through 7-14-21 days (chronic stress). At the end of exposure periods, total RNA was purified from the cortex and hippocampus. The amounts of beta-actin, APP and MAPK-1 mRNA were determined with real time PCR method. Our results indicate that the mRNA expression of beta-actin and APP followed a U-shaped time-response curve. Both acute and chronic IS caused a significant increase in beta-actin and MAPK-1 mRNA expression. Significant APP mRNA elevation was observed only by the 3rd week after RS. Our findings demonstrate that both acute and chronic IS lead to gene transcriptional changes of beta-actin, APP and MAPK-1. These proteins maintain the normal function of the cytoskeleton and the synaptic plasticity. The above changes may lead to cognitive deterioration, and the development of AD.
Szegedi Tudományegyetem, Pszichiátriai Klinika, Alzheimer-kór Kutatócsoport, Szeged. santha.petra@gmail.com
Petra
P
Sántha
Magdolna
M
Pákáski
Orsike
O
Fazekas
Szabina
S
Szucs
Eszter Klára
EK
Fodor
János
J
Kálmán
Sára
S
Kálmán
Gyula
G
Szabó
Zoltán
Z
Janka
János
J
Kálmán
D000199:Actins / Q000235:genetics* / Q000378:metabolism; D000208:Acute Disease; D000544:Alzheimer Disease / Q000235:genetics* / Q000503:physiopathology* / Q000523:psychology; D016564:Amyloid beta-Protein Precursor / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002908:Chronic Disease; D007103:Immobilization; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics* / Q000378:metabolism; D009473:Neuronal Plasticity / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D013315:Stress, Psychological / Q000150:complications / Q000235:genetics*; D014158:Transcription, Genetic
D000199:Actins; D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger; C535149:Mapk1 protein, rat; D019950:Mitogen-Activated Protein Kinase 1