iTRAQ: a method to elucidate cellular responses to mycotoxin zearalenone.
Mycotoxin zearalenone (ZEN) is a secondary metabolite produced by some Fusarium species that contaminate a large variety of grains and feedstuffs worldwide. ZEN has been associated with a wide variety of adverse health effects including hepatotoxic, hematologic, immunotoxic and genotoxic. In order to better understand the mechanism of ZEN toxicity, a proteomic approach was applied to characterize cellular responses of hepatocarcinoma cells (HepG2) to ZEN exposure. Protein extracts from cultured HepG2 cells treated with 100 µm ZEN for 8 h, as well as extracts from control cells. The screening method applied to compare the proteome was based on the stable isotope approach of isobaric tagging for relative and absolute quantification (iTRAQ). This study identified 982 proteins, among which peptides and their corresponding proteins were identified and quantified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Ingenuity pathways analysis software was then used to determine the biological functions and canonical pathways associated with the ZEN-responsive proteins.
Laboratory of Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, Monastir, 5000, Tunisia.
Amel Chatti
AC
Gazzah
Luc
L
Camoin
Salwa
S
Abid
Hassen
H
Bacha
Moncef
M
Ladjimi
D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology; D013058:Mass Spectrometry; D009183:Mycotoxins / Q000633:toxicity*; D010455:Peptides / Q000378:metabolism; D011506:Proteins / Q000737:chemistry; D020543:Proteome / Q000187:drug effects; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D015025:Zearalenone / Q000633:toxicity*
D002273:Carcinogens; D007202:Indicators and Reagents; D009183:Mycotoxins; D010455:Peptides; D011506:Proteins; D020543:Proteome; D015025:Zearalenone