[Synergistic interactions of TRAIL and paclitaxel on the nasopharyngeal carcinoma cell lines in vitro].
OBJECTIVE
To investigate the synergistic cytotoxicity of TRAIL and paclitaxel on nasopharyngeal cell lines CNE-1 and CNE-2.


METHODS
CCK-8 assays the growth inhibition rate of CNE-1 and CNE-2 which was treated with TRAIL or paclitaxel or combination of both. Flow cytometry tests the apoptosis rate of CNE-1 and CNE-2 which was treated with TRAIL or paclitaxel or combination of each other.


RESULTS
In certain range of time and concentration,TRAIL and paclitaxel inhibited the growth of the cell lines of CNE-1 and CNE-2 in a time-dose dependent manner (P < 0.05). The rate of growth inhibition and apoptosis in TRAIL and paclitaxel combinative group was more significant than that in the TRAIL and paclitaxel singular group (P < 0.05).


CONCLUSIONS
TRAIL and paclitaxel had a synergistic killing effect on NPC cell lines and showed better affection than singular group, which provides a novel and prospective strategy for NPC chemotherapy.
Department of Otorhinolaryngology, Xiangya Hospital, Central South University, Changsha 410008, China.
Pingqing
P
Tan
Yong
Y
Liu
Yuanzheng
Y
Qiu
Shisheng
S
Li
Ying
Y
Guo
Gao
G
Li
Yongquan
Y
Tian
Xin
X
Zhang
Donghai
D
Huang
Jianyun
J
Xiao
D017209:Apoptosis / Q000187:drug effects*; D002277:Carcinoma; D045744:Cell Line, Tumor; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology*; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology*
D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D017239:Paclitaxel