Quantitative analysis of miroestrol and kwakhurin for standardisation of Thai miracle herb 'Kwao Keur' (Pueraria mirifica) and establishment of simple isolation procedure for highly estrogenic miroestrol and deoxymiroestrol.
Quantitative analysis of miroestrol (1) and kwakhurin (3) by HPLC, leading to standardisation of commercially available Thai miracle herb 'Kwao Keur' which has been identified with Pueraria mirifica, was established using independent solvent systems. The simple isolation procedure of highly estrogenic miroestrol (1) and deoxymiroestrol (2) from P. mirifica was also proposed.
Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8675, Japan.
Satoko
S
Shimokawa
Takuya
T
Kumamoto
Tsutomu
T
Ishikawa
Miho
M
Takashi
Yoshihiro
Y
Higuchi
Chaiyo
C
Chaichantipyuth
Sunee
S
Chansakaow
D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification; D007529:Isoflavones / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification*
D003374:Coumarins; D007529:Isoflavones; D013256:Steroids; C406602:deoxymiroestrol; C511898:kwakhurin; C571572:miroestrol