Dynamic compression promotes proliferation and neovascular networks of endothelial progenitor cells in demineralized bone matrix scaffold seed.
Neovascularization is required for bone formation and successful fracture healing. In the process of neovascularization, endothelial progenitor cells (EPCs) play an important role and finish vascular repair through reendothelialization to promote successful fracture healing. In this study, we found that dynamic compression can promote the proliferation and capillary-like tube formation of EPCs in the demineralized bone matrix (DBM) scaffold seed. EPCs isolated from the bone marrow of rats have been cultured in DBM scaffolds before dynamic compression and then seeded in the DBM scaffolds under dynamic conditions. The cells/scaffold constructs were subjected to cyclic compression with 5% strain and at 1 Hz for 4 h/day for 7 consecutive days. By using MTT and real-time PCR, we found that dynamic compression can significantly induce the proliferation of EPCs in three-dimensional culture with an even distribution of cells onto DBM scaffolds. Both in vitro and in vivo, the tube formation assays in the scaffolds showed that the loaded EPCs formed significant tube-like structures. These findings suggest that dynamic compression promoted the vasculogenic activities of EPCs seeded in the scaffolds, which would benefit large bone defect tissue engineering.
Department of Orthopaedics, Shengjing Hospital of China Medical University, Shenyang, China.
Zhan
Z
Kong
Jianjun
J
Li
Qun
Q
Zhao
Zhendong
Z
Zhou
Xiangnan
X
Yuan
Dongxiang
D
Yang
Xu
X
Chen
D000818:Animals; D019149:Bioreactors; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism* / Q000637:transplantation / Q000648:ultrastructure; D005786:Gene Expression Regulation; D040542:Mechanotransduction, Cellular; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic / Q000235:genetics; D011312:Pressure; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism* / Q000648:ultrastructure; D013552:Swine; D013997:Time Factors; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics / Q000378:metabolism; D014841:von Willebrand Factor / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D014841:von Willebrand Factor; D040301:Vascular Endothelial Growth Factor Receptor-2