[The influence of endothelin-1 and endothelin receptor blocker on the content of white blood cells in the peripheral blood of rat].
The aim of the study was to evaluate the influence of endothelin-1 (ET-1) and endothelin receptor (ETA) blocker (BQ 123) on the content of white blood cells in the peripheral blood of rat.


METHODS
Experiments were performed on Wistar rats divided into following groups: group I (control) received saline; group II (ET-1) received endothelin-1 (3 microg/kg b.w.); group III (BQ 123) received ETA receptor blocker (1 mg/kg b.w.); group IV (BQ 123+ET-1) received BQ 123 (1 mg/kg b.w) and 30 minutes later ET-1 (3 microg/kg b.w.). Peripheral blood was collected from animals at three time intervals to determine the content of white blood cells using a hematology analyzer MICROS OT 45.


RESULTS
Administration of ET-1 resulted in a statistically significant increase in the concentration of white blood cells after 1 hour after administration, compared with the control group. Contrast, administration of a specific blocker of ETA (BQ 123) reduced the leukocyte content after 1 and 5 hours after administration of ET-1.


CONCLUSIONS
Intravenous administration of ET-1 significantly increased the content of white blood cells in peripheral blood of rat via the ETA receptor since reversed its blockage caused the change.
Uniwersytet Medyczny w Łodzi, Katedra Fizjologii Doświadczalnej i Klinicznej, Zakład Fizjologii Układu Krazenia, Mazowiecka. kleniewska.p@interia.pl
Paulina
P
Kleniewska
Łukasz
Ł
Michalski
Beata
B
Skibska
Anna
A
Goraca
D000818:Animals; D065130:Endothelin A Receptor Antagonists; D019332:Endothelin-1 / Q000008:administration & dosage* / Q000037:antagonists & inhibitors*; D007275:Injections, Intravenous; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D065130:Endothelin A Receptor Antagonists; D019332:Endothelin-1