Colony to colorimetry in 6 h: ELISA detection of a surface-expressed Pseudomonas aeruginosa virulence factor using immobilized bacteria.
A rapid ELISA employing intact Pseudomonas aeruginosa (PA) is described that allows discrimination between strains harboring flagellin type a or b. All 52 PA strains known to harbor flagellin type b were positive in this ELISA when screened with a fully human monoclonal antibody (LST-007) targeting flagellin type b. Completion of this assay in only 6 h, from picking a single bacterial colony to a colorimetric product, could easily be adapted to a clinical laboratory setting and permit the appropriate choice of therapeutic monoclonal antibody versus its homologous flagellin target in PA-infected patients.
Lostam BioPharmaceuticals Ltd., Nazareth Illit, 17111, Israel.
Azmi
A
Adawi
Lewis F
LF
Neville
D001431:Bacteriological Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005408:Flagellin / Q000032:analysis*; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D013997:Time Factors
D005408:Flagellin