A molecular epidemiological and genetic diversity study of tuberculosis in Ibadan, Nnewi and Abuja, Nigeria.
BACKGROUND
Nigeria has the tenth highest burden of tuberculosis (TB) among the 22 TB high-burden countries in the world. This study describes the biodiversity and epidemiology of drug-susceptible and drug-resistant TB in Ibadan, Nnewi and Abuja, using 409 DNAs extracted from culture positive TB isolates.


RESULTS
DNAs extracted from clinical isolates of Mycobacterium tuberculosis complex were studied by spoligotyping and 24 VNTR typing. The Cameroon clade (CAM) was predominant followed by the M. africanum (West African 1) and T (mainly T2) clades. By using a smooth definition of clusters, 32 likely epi-linked clusters related to the Cameroon genotype family and 15 likely epi-linked clusters related to other "modern" genotypes were detected. Eight clusters concerned M. africanum West African 1. The recent transmission rate of TB was 38%. This large study shows that the recent transmission of TB in Nigeria is high, without major regional differences, with MDR-TB clusters. Improvement in the TB control programme is imperative to address the TB control problem in Nigeria.
Zankli Medical Centre, Abuja, Nigeria.
Lovett
L
Lawson
Jian
J
Zhang
Michel K
MK
Gomgnimbou
Saddiq T
ST
Abdurrahman
Stéphanie
S
Le Moullec
Fatima
F
Mohamed
Gertrude N
GN
Uzoewulu
Olumide M
OM
Sogaolu
Khye Seng
KS
Goh
Nnamdi
N
Emenyonu
Guislaine
G
Refrégier
Luis E
LE
Cuevas
Christophe
C
Sola
D000293:Adolescent; D000328:Adult; D000368:Aged; D016000:Cluster Analysis; D019143:Evolution, Molecular; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009549:Nigeria / Q000453:epidemiology; D010802:Phylogeny; D014376:Tuberculosis / Q000453:epidemiology* / Q000635:transmission; D018088:Tuberculosis, Multidrug-Resistant / Q000235:genetics; D055815:Young Adult