Effects of syringe type and storage conditions on results of equine blood gas and acid-base analysis.
OBJECTIVE
To determine effects of syringe type and storage conditions on blood gas and acid-base values for equine blood samples.


METHODS
Blood samples obtained from 8 healthy horses.


METHODS
Heparinized jugular venous blood was equilibrated via a tonometer at 37°C with 12% O(2) and 5% CO(2). Aliquots (3 mL) of tonometer-equilibrated blood were collected in random order by use of a glass syringe (GS), general-purpose polypropylene syringe (GPPS), or polypropylene syringe designed for blood gas analysis (PSBGA) and stored in ice water (0°C) or at room temperature (22°C) for 0, 5, 15, 30, 60, or 120 minutes. Blood pH was measured, and blood gas analysis was performed; data were analyzed by use of multivariable regression analysis.


RESULTS
Blood Po(2) remained constant for the reference method (GS stored at 0°C) but decreased linearly at a rate of 7.3 mm Hg/h when stored in a GS at 22°C. In contrast, Po(2) increased when blood was stored at 0°C in a GPPS and PSBGA or at 22°C in a GPPS; however, Po(2) did not change when blood was stored at 22°C in a PSBGA. Calculated values for plasma concentration of HCO(3) and total CO(2) concentration remained constant in the 3 syringe types when blood was stored at 22°C for 2 hours but increased when blood was stored in a GS or GPPS at 0°C.


CONCLUSIONS
Blood samples for blood gas and acid-base analysis should be collected into a GS and stored at 0°C or collected into a PSBGA and stored at room temperature.
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
Sarah A
SA
Kennedy
Peter D
PD
Constable
Ismail
I
Sen
Laurent
L
Couëtil
D000818:Animals; D001784:Blood Gas Analysis / Q000379:methods / Q000662:veterinary*; D005260:Female; D006736:Horses / Q000097:blood*; D008297:Male; D015999:Multivariate Analysis; D011897:Random Allocation; D012044:Regression Analysis; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*; D013594:Syringes / Q000662:veterinary*