Simple and reproducible hepatectomy in the mouse using the clip technique.
OBJECTIVE
To investigate the reliability of massive hepatectomy models by using clip techniques.


METHODS
We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction > 70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves, and histopathological findings.


RESULTS
According to anatomical results, models with 75%, 80%, and 90% hepatectomy produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques.


CONCLUSIONS
Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.
Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, United States. hori.tomohide@mayo.edu
Tomohide
T
Hori
Norifumi
N
Ohashi
Feng
F
Chen
Ann-Marie T
AM
Baine
Lindsay B
LB
Gardner
Toshiyuki
T
Hata
Shinji
S
Uemoto
Justin H
JH
Nguyen
D000818:Animals; D006498:Hepatectomy / Q000295:instrumentation* / Q000379:methods*; D059032:Learning Curve; D008099:Liver / Q000473:pathology / Q000601:surgery*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D009336:Necrosis; D009929:Organ Size; D015203:Reproducibility of Results; D013525:Surgical Instruments; D013536:Suture Techniques; D013997:Time Factors