Nanowire-integrated microfluidic devices for facile and reagent-free mechanical cell lysis.
Cell lysis is an essential task for the detection of intracellular components. In this work, we introduce novel microfluidic devices integrated with patterned one-dimensional nanostructure arrays for facile and high-throughput mechanical cell lysis. The geometry of the hydrothermally grown ZnO nanowires, characterised by sharp tips and high aspect ratios, aids in anchoring the cell and tearing the plasma membrane, enabling simple and highly efficient extraction of cellular proteins and nucleic acids. This method lyses cells more effectively than conventional chemical lysis methods with simpler equipment and a shorter processing time.
Multifunctional and Integrated Nanosystem Technology (MINT) Group, School of Mechanical, Aerospace and Systems Engineering, Division of Mechanical Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, Korea.
Jung
J
Kim
Jung Woo
JW
Hong
Dong Pyo
DP
Kim
Jennifer H
JH
Shin
Inkyu
I
Park
D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D053770:Nanowires / Q000737:chemistry*; D009696:Nucleic Acids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D015034:Zinc Oxide / Q000737:chemistry
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D011506:Proteins; D015034:Zinc Oxide