Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage.
Exposure to high levels of different environmental pollutants is known to be associated with induction of DNA damage in humans. Thus DNA repair is of great importance in preventing mutations and contributes crucially to the prevention of cancer. In our study we have focused on quantitative analysis of Gentiana asclepiadea aqueous or methanolic extracts obtained from flower and haulm, their antioxidant potency in ABTS post-column derivatisation, and their potential ability to enhance DNA repair in human lymphocytes after hydrogen peroxide (H(2)O(2)) treatment (250 μM, 5 min). We also studied DNA repair in human kidney HEK 293 cells after exposure to 20 nm silver nanoparticles (AgNPs) (100 μg/ml, 30 min) in the presence and absence of the plant extract. We have found that mangiferin along with unidentified polar compounds are the most pronounced antioxidants in the studied extracts. Extract from haulm exhibited slightly stronger antioxidant properties compared to flower extracts. However, all four extracts showed significant ability to enhance DNA repair in both cell types after H(2)O(2) and AgNP treatments.
Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina, Bratislava 842 15, Slovakia.
Alexandra
A
Hudecová
Barbara
B
Kusznierewicz
Katarína
K
Hašplová
Anna
A
Huk
Zuzana
Z
Magdolenová
Eva
E
Miadoková
Eliška
E
Gálová
Mária
M
Dušinská
D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D004260:DNA Repair; D030012:Gentiana / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D053768:Metal Nanoparticles; D010936:Plant Extracts / Q000494:pharmacology*; D012834:Silver / Q000737:chemistry*
D000975:Antioxidants; D010936:Plant Extracts; D012834:Silver; D006861:Hydrogen Peroxide