Development of a multiplex PCR assay for rapid identification of Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex.
We have developed a multiplex PCR assay for rapid identification and differentiation of cultures for Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex. The assay is valuable for use in clinical and veterinary laboratories, and in a deployable laboratory during outbreaks.
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia. kohsf.adrian@gmail.com
Seng Fook
SF
Koh
Sun Tee
ST
Tay
Rasana
R
Sermswan
Surasakdi
S
Wongratanacheewin
Kek Heng
KH
Chua
Savithri D
SD
Puthucheary
D042602:Burkholderia cepacia complex / Q000235:genetics*; D042726:Burkholderia mallei / Q000235:genetics*; D016957:Burkholderia pseudomallei / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D058889:Molecular Typing; D060885:Multiplex Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S