[Improved expression of human rotavirus VP6 antigen in the recombinant adenoviruse by codon optimization].
OBJECTIVE
To increase the recombinant adenovirus vector mediated human rotavirus VP6 gene expression through coden optimization.


METHODS
We have artificially synthesized VP6 gene of group A human rotavirus according to the human biased codon. The modified gene was transfected into 293 cells using adenovirus vector and the gene product, the respective protein was produced. The expression level of optimized gene and wild type gene was detected by Immunofluorescence (IF) and Western Blot.


RESULTS
A remarkable increase of the expression level of optimized VP6 gene in comparison with the wild-type control.


CONCLUSIONS
The coden optimization indeed help increasing the recombinant adenovirus mediated human rotavirus gene expression, which indicated the potential application of such recombinant adenoviruses in the development of adenoviral-vectored rotavirus vaccines.
Clinical Laboratory of Peking Union Medical College Hospital, Beijing 100073, China.
Da-Wei
DW
Tong
Jing-Dong
JD
Song
Jian-Wei
JW
Wang
Min
M
Wang
D000256:Adenoviridae / Q000235:genetics; D000956:Antigens, Viral / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics*; D003062:Codon; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis*
D000956:Antigens, Viral; D036022:Capsid Proteins; D003062:Codon; D011994:Recombinant Proteins; C061332:VP6 protein, Rotavirus