Toward the identification and regulation of the Arabidopsis thaliana ABI3 regulon.
The plant-specific, B3 domain-containing transcription factor ABSCISIC ACID INSENSITIVE3 (ABI3) is an essential component of the regulatory network controlling the development and maturation of the Arabidopsis thaliana seed. Genome-wide chromatin immunoprecipitation (ChIP-chip), transcriptome analysis, quantitative reverse transcriptase-polymerase chain reaction and a transient promoter activation assay have been combined to identify a set of 98 ABI3 target genes. Most of these presumptive ABI3 targets require the presence of abscisic acid for their activation and are specifically expressed during seed maturation. ABI3 target promoters are enriched for G-box-like and RY-like elements. The general occurrence of these cis motifs in non-ABI3 target promoters suggests the existence of as yet unidentified regulatory signals, some of which may be associated with epigenetic control. Several members of the ABI3 regulon are also regulated by other transcription factors, including the seed-specific, B3 domain-containing FUS3 and LEC2. The data strengthen and extend the notion that ABI3 is essential for the protection of embryonic structures from desiccation and raise pertinent questions regarding the specificity of promoter recognition.
Department of Molecular Genetics, Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK) Corrensstr. 3, D-06466 Gatersleben, Germany.
Gudrun
G
Mönke
Michael
M
Seifert
Jens
J
Keilwagen
Michaela
M
Mohr
Ivo
I
Grosse
Urs
U
Hähnel
Astrid
A
Junker
Bernd
B
Weisshaar
Udo
U
Conrad
Helmut
H
Bäumlein
Lothar
L
Altschmied
D017360:Arabidopsis / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D018744:DNA, Plant / Q000737:chemistry / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D059372:Nucleotide Motifs; D011401:Promoter Regions, Genetic; D018085:Regulon; D012639:Seeds / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
C091601:ABI3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018744:DNA, Plant; D014157:Transcription Factors