Enhanced immunomodulatory activity and stability in simulated digestive juices of Lactobacillus plantarum L-137 by heat treatment.
This study reports the effect of heat treating Lactobacillus plantarum L-137 on its in vitro cytokine-inducing activity, on the stability of this activity in simulated digestive juices, and on its in vivo immunomodulatory properties. L-137 cells were harvested at the stationary phase with or without the subsequent heat treatment and then lyophilized. Heat-killed L-137 cells stimulated mouse spleen cells to produce more interleukin-12p40 than unheated L-137. The interleukin-12p40-inducing activity of unheated L-137 was significantly lower when incubated with simulated intestinal juice, but the activity of heat-killed L-137 cells was maintained. Furthermore, heat-killed L-137 was more protective than unheated L-137 in a mouse model of dextran sulfate sodium-induced colitis. A heat treatment may therefore be effective for enhancing the immunomodulatory activity of L-137 cells.
Food Science Research Center, House Wellness Foods Corporation, Itami, Japan. Fujiki_Takashi@house-wf.co.jp
Takashi
T
Fujiki
Yoshitaka
Y
Hirose
Yoshihiro
Y
Yamamoto
Shinji
S
Murosaki
D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D003092:Colitis / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D016264:Dextran Sulfate / Q000008:administration & dosage; D005260:Female; D005612:Freeze Drying; D005750:Gastric Juice / Q000737:chemistry; D006358:Hot Temperature; D007155:Immunologic Factors / Q000494:pharmacology*; D053762:Interleukin-12 Subunit p40 / Q000096:biosynthesis / Q000276:immunology; D007419:Intestinal Secretions / Q000737:chemistry; D048191:Lactiplantibacillus plantarum / Q000737:chemistry*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019936:Probiotics / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
D007155:Immunologic Factors; D053762:Interleukin-12 Subunit p40; D016264:Dextran Sulfate