AxyR, an AraC family transcriptional activator of the xylanase 3 gene, requires xylo-oligosaccharides as a cofactor for DNA binding in Paenibacillus sp. strain W-61.
The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.
Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Mutsumi
M
Fukuda
Shigeki
S
Yoshida
Hiroya
H
Itoh
Yoji
Y
Kato
Seiji
S
Watanabe
Yoshifumi
Y
Itoh
Yoshiyuki
Y
Kamio
Jun
J
Kaneko
D051777:AraC Transcription Factor / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D003067:Coenzymes / Q000378:metabolism*; D004187:Disaccharides / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000378:metabolism*; D056507:Paenibacillus / Q000201:enzymology* / Q000235:genetics; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014312:Trisaccharides / Q000378:metabolism; D014990:Xylans / Q000378:metabolism*
D051777:AraC Transcription Factor; D001426:Bacterial Proteins; D003067:Coenzymes; D004187:Disaccharides; D014312:Trisaccharides; D014990:Xylans; C515044:xylotriose; D043364:Endo-1,4-beta Xylanases; C004173:xylobiose