The importance of immunohistochemical evaluation of the vascular changes from the decidua and placenta in recurrent pregnancy loss.
The angiogenesis is a complex process, incompletely understood, regulated by various stimulating and inhibiting angiogenic factors. In the present study, we proposed to evaluate the angiogenic changes that occur in the cases with recurrent pregnancy loss comparing with a control group represented by women with requested abortion. The evaluation of the changes in the vascular bed was made by immunohistochemical methods, evaluating the answer of the curettage products to the mouse anti-human CD31 and CD34 monoclonal antibodies immunolabeling. The endothelial cells reaction to the CD31 antibody was different, very intense in the normal or slightly congestive vessels. The endothelial cells from the strongly congestive vessels had a light and scratchy reaction. We found intense positive reactions in the control group for CD34 in the vessels from the villous axis and also in the vessels from the spongious decidua. In the study group, we found light positive reaction in the vessels from the decidua situated in the proximity of the necrotic areas; we found a light positive reaction also in the vessels and mesenchymal fibroblasts from some chorial villous axis.
Department of Obstetrics and Gynecology, University of Medicine and Pharmacy of Craiova, Craiova, Romania. manoleamaria@gmail.com
Maria Magdalena
MM
Manolea
Olguţa Alice
OA
Gavrilă
Florina Carmen
FC
Popescu
Liliana
L
Novac
Garofiţa Olivia
GO
Mateescu
D000026:Abortion, Habitual / Q000473:pathology*; D000818:Animals; D003656:Decidua / Q000098:blood supply* / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D010920:Placenta / Q000098:blood supply* / Q000276:immunology; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012307:Risk Factors