A novel germline mutation in HOXB13 is associated with prostate cancer risk in Chinese men.
BACKGROUND
A rare mutation G84E in HOXB13 was recently identified to be associated with prostate cancer (PCa) in Caucasians. The goal of this study is to test association between HOXB13 genetic variants and PCa risk in Chinese men.


METHODS
All study subjects were part of the Chinese Consortium for Prostate Cancer Genetics (ChinaPCa). In the first stage, we screened for mutations by sequencing the HOXB13 coding region in 96 unrelated PCa patients. In stage 2, G84E and novel mutations found in stage 1 were genotyped in 671 PCa patients and 1,536 controls. In stage 3, mutation status in 751 additional PCa patients was imputed via haplotype.


RESULTS
The G84E mutation was not detected in this study. However, a novel mutation, G135E, was identified among 96 patients in stage 1. It was also observed twice in 575 additional PCa patients but not in 1,536 control subjects of stage 2. The frequency of G135E was significantly different between cases and controls, with a P-value of 0.027, based on Fisher's exact test. Haplotype estimation showed that G135E mutation carriers shared a unique haplotype that was not observed in other subjects. In stage 3, two more PCa patients were predicted to carry the G135E mutation.


CONCLUSIONS
We identified a novel rare mutation in the HOXB13 gene, G135E, which appears to be a founder mutation. This mutation is associated with increased PCa risk in Chinese men. Consistent with a previous report, our findings provide further evidence that rare mutations in HOXB13 contribute to PCa risk.
Fudan-VARI Center for Genetic Epidemiology, School of Life Sciences, Fudan University, Shanghai, PR China.
Xiaoling
X
Lin
Lianxi
L
Qu
Zhuo
Z
Chen
Chuanliang
C
Xu
Dingwei
D
Ye
Qiang
Q
Shao
Xiang
X
Wang
Jun
J
Qi
Zhiwen
Z
Chen
Fangjian
F
Zhou
Meilin
M
Wang
Zhong
Z
Wang
Dalin
D
He
Denglong
D
Wu
Xin
X
Gao
Jianlin
J
Yuan
Gongxian
G
Wang
Yong
Y
Xu
Guozeng
G
Wang
Pei
P
Dong
Yang
Y
Jiao
Jin
J
Yang
Jun
J
Ou-Yang
Haowen
H
Jiang
Yao
Y
Zhu
Shancheng
S
Ren
Zhengdong
Z
Zhang
Changjun
C
Yin
Qijun
Q
Wu
Ying
Y
Zheng
Aubrey R
AR
Turner
Sha
S
Tao
Rong
R
Na
Qiang
Q
Ding
Daru
D
Lu
Rong
R
Shi
Jielin
J
Sun
Fang
F
Liu
S Lilly
SL
Zheng
Zengnan
Z
Mo
Yinghao
Y
Sun
Jianfeng
J
Xu
D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution / Q000235:genetics; D044466:Asian People / Q000235:genetics*; D002681:China / Q000453:epidemiology; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D018095:Germ-Line Mutation / Q000235:genetics*; D018698:Glutamic Acid / Q000235:genetics; D005998:Glycine / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*
C484414:HOXB13 protein, human; D018398:Homeodomain Proteins; D018698:Glutamic Acid; D005998:Glycine