Effects of phthalates on the human corneal endothelial cell line B4G12.
Phthalates are industrial chemicals used in many cosmetics. We evaluated an in vitro model for eye irritancy testing using the human corneal endothelial cell line B4G12. Cell proliferation and toxicity were assessed after exposing to di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisodecyl phthalate (DIDP), di-n-octyl phthalate (DnOP), and di-isononyl phthalate (DINP). Gene expression and secretion of inflammatory cytokines were evaluated after exposure to DBP. Decreased cell proliferation was observed for the phthalates DBP, BBP, and DEHP, and cell toxicity was observed for DBP and BBP. Upon DBP exposure at nontoxic concentrations, a significant increased gene expression and cytokine cell secretion were observed for interleukin-1β (IL-1β) and IL-8, and also an increased IL-6 secretion was observed. In conclusion, the human corneal endothelial cell line B4G12 may be a potential model for inflammatory eye irritancy testing of phthalates.
Department of Public Health, Centre of Arctic Health & Unit of Cellular and Molecular Toxicology, Aarhus University, Aarhus County, Denmark.
Tanja
T
Krüger
Yi
Y
Cao
Søren K
SK
Kjærgaard
Lisbeth E
LE
Knudsen
Eva C
EC
Bonefeld-Jørgensen
D000826:Animal Testing Alternatives; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003124:Colorimetry / Q000379:methods; D003993:Dibutyl Phthalate / Q000633:toxicity; D004051:Diethylhexyl Phthalate / Q000633:toxicity; D042783:Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D004728:Endothelium, Corneal / Q000166:cytology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010795:Phthalic Acids / Q000633:toxicity*; D010968:Plasticizers / Q000633:toxicity*; D060888:Real-Time Polymerase Chain Reaction
D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D010795:Phthalic Acids; D010968:Plasticizers; D003993:Dibutyl Phthalate; C010715:di-n-octyl phthalate; D004051:Diethylhexyl Phthalate; D007770:L-Lactate Dehydrogenase; C042080:diisodecyl phthalate; C027561:butylbenzyl phthalate