A simple technique for long-term preservation of leptospires.
The viability of six serovars of Leptospira spp. was studied after long storage at -70°C. The bacteria were either preserved in Ellinghausen-McCullough-Johnson-Harris (EMJH) liquid growth medium or in sheep blood added as a cryoprotectant. The viability of the strains was observed on a monthly basis by dark-ground microscopy over a period of 20 months at -70°C. Addition of sheep blood was not significantly advantageous, since leptospires that were stored in EMJH showed a slight increase in number after recovery. The results suggest a very simple and useful technique for long-term preservation of such Leptospira.
Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt. ahmedsamir121@hotmail.com
Ahmed
A
Samir
Momtaz O
MO
Wasfy
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D007919:Leptospira / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000502:physiology*; D050296:Microbial Viability; D011309:Preservation, Biological / Q000379:methods*; D012703:Serotyping
D003470:Culture Media