Mitogen activated protein kinases blockade improves lipopolysaccharide-induced ileal motor disturbances.
BACKGROUND
several diseases such as sepsis can affect the ileum. Lipopolysaccharide (LPS), an endotoxin present in the cell wall of gram negative bacteria, is a causative agent of sepsis.


OBJECTIVE
the aims of this study were: a) to investigate the role of mitogen activated protein kinases (MAPKs) in the effect of LPS on the acetylcholine-induced contractions of rabbit ileum; and b) to study the localization of MAPKs in the ileum.


METHODS
ileal contractility was studied in an organ bath and MAPKs were localized by immunohistochemistry.


RESULTS
acetylcholine-induced contractions decreased with LPS. SB203580, SP600125 and U0126 blocked the effect of LPS on the acetylcholine-induced contractions. Phosphorylated p38 and ERK were detected in neurons of myenteric plexus and Phosphorylated p38 and JNK in smooth muscle cells of ileum.


CONCLUSIONS
we can suggest that p38, JNK, and ERK MAPKs are involved in the mechanism of action of LPS in the ileum.
Department of Pharmacology and Physiology, Unit of Physiology, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.
Sergio
S
Gonzalo
Laura
L
Grasa
Ligia Verónica
LV
Hernández
María Pilar
MP
Arruebo
Miguel Ángel
MÁ
Plaza
María Divina
MD
Murillo
D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D002070:Butadienes / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D007082:Ileum / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology*; D007093:Imidazoles / Q000494:pharmacology; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009570:Nitriles / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D011817:Rabbits; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000873:Anthracenes; D015415:Biomarkers; D002070:Butadienes; D004791:Enzyme Inhibitors; D007093:Imidazoles; D008070:Lipopolysaccharides; D009570:Nitriles; D011725:Pyridines; C113580:U 0126; C440499:lipopolysaccharide, Escherichia coli O111 B4; C432165:pyrazolanthrone; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D000109:Acetylcholine; C093642:SB 203580