Nano-/microfiber scaffold for tissue engineering: physical and biological properties.
Alginate hydrogel (AH) has intrinsic physical and biological limitations that hinder its broader application in tissue engineering. We hypothesized that the inclusion of nanofibers in the hydrogel and the use of a biotemplate that mimics nature would enhance the translational potential of alginate hydrogels. In this study, we have shown a method to obtain nano-/microfibers of titanium (nfTD) and hydroxyapatite (nfHY) using cotton as a biotemplate. These fibers were incorporated in the alginate hydrogel and the mechanical characteristics and biological response to these reinforced materials were evaluated. We observed that these nanofibers resembled the structure of natural collagen and did not mediate cell toxicity. The incorporation of nfTD or nfHY to the AH has not increased the viscosity of the hydrogel. Therefore, this is a feasible method to produce a scaffold with improved physical characteristics, while at the same time generating an enhanced environment for cell adhesion and proliferation.
Department of Operative Dentistry School of Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil. biancapalmasantana.mg@gmail.com
Bianca Palma
BP
Santana
Gian Francesco dos Reis
GF
Paganotto
Fernanda
F
Nedel
Evandro
E
Piva
Rodrigo Varella
RV
de Carvalho
Jacques Eduardo
JE
Nör
Flávio Fernando
FF
Demarco
Neftali Lenin Villarreal
NL
Carreño
D016475:3T3 Cells; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002470:Cell Survival; D017886:Durapatite / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; D017886:Durapatite; D014025:Titanium