Anti-inflammatory effects of Calophyllum inophyllum L. in RAW264.7 cells.
Calophyllum inophyllum L. has been used as folk medicine in the treatment             of ocular burn and it has demonstrated potential to be an anti-inflammatory agent.             The aim of this study was to explore the anti-inflammatory activities of an acetone             extract of C. inophyllum L. leaves (CIL). The CIL extract was tested on lipopolysaccharide             (LPS)-induced RAW 264.7 cells to evaluate the effect of CIL extract on the expression             of nitric oxide (NO) and inducible nitric oxide synthase (iNOS). Results showed             that the CIL extract markedly suppressed the LPS-induced production of nitric             oxide, as well as the expression of iNOS, cyclooxygenase (COX)-2 and nuclear factor-kappaB             (NF-κB) in a dose-dependent manner. LPS-induced microRNA (miR)-146a expression             was inhibited by CIL extract, while miR-155 and miR-424 expression was not affected             as demonstrated using quantitative RT-PCR analysis. Taken together, these observations             show that CIL extract has anti-inflammatory effect, which extends the potential             application for prevention of inflammatory diseases, and its mechanism may be             partially associated with blocking COX-2 and iNOS of RAW 264.7 cells.
Department of Biological Science and Technology, School of Pharmacy, China Medical University, Taichung 40402, Taiwan, ROC.
Shih-Chang
SC
Tsai
Yu-Hsin
YH
Liang
Jo-Hua
JH
Chiang
Fon-Chang
FC
Liu
Wen-Hsin
WH
Lin
Shu-Jen
SJ
Chang
Wei-Yu
WY
Lin
Chieh-Hsi
CH
Wu
Jing-Ru
JR
Weng
D021581:Active Transport, Cell Nucleus; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D044946:Biflavonoids / Q000737:chemistry / Q000302:isolation & purification; D029759:Calophyllum / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D009828:Oleanolic Acid / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D044946:Biflavonoids; D008070:Lipopolysaccharides; D035683:MicroRNAs; C534190:Mirn146 microRNA, mouse; C532623:Mirn155 microRNA, mouse; D010936:Plant Extracts; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; D009569:Nitric Oxide; D009828:Oleanolic Acid; C011164:amentoflavone; D052247:Nitric Oxide Synthase Type II; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2