Change in mutagenic activity of genistein after a nitrite treatment.
This study examined the mutagenic activity of genistein after a nitrite treatment under acidic conditions. Nitrite-treated genistein exhibited mutagenic activity toward Salmonella typhimurium strains TA 100 and TA 98 with or without S9 mix. Nitrite-treated genistein was demonstrated by electron spin resonance to generate radicals. An instrumental analysis showed 3'-nitro-genistein to have been formed in the reaction mixture. However, 3'-nitro-genistein did not exhibit mutagenic activity toward the S. typhimurium strains, suggesting that other mutagens might also have been formed in the reaction mixture. The clastogenic properties of nitrite-treated genistein and 3'-nitro-genistein were examined by a micronucleus test with male ICR mice. Nitrite-treated genistein and 3'-nitro-genistein showed a significantly higher frequency of micronucleated reticulocytes in mice than in the control group. These results suggest that a daily oral intake of genistein and nitrite through foodstuffs might induce the formation of various mutagenic compounds in the body.
School of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka, Japan. masudas@u-shizuoka-ken.ac.jp
Shuichi
S
Masuda
Yuko
Y
Shimamura
Tatsuya
T
Kato
Tan
T
Yu-Feng
Ken-ichi
K
Iwamoto
Naohide
N
Kinae
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000378:metabolism; D019833:Genistein / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity*; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D009573:Nitrites / Q000737:chemistry*; D012156:Reticulocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development
D005609:Free Radicals; D009573:Nitrites; D019833:Genistein