Serological analysis of SEREX-defined medullary breast carcinoma-associated antigens.
Medullary breast carcinoma (MBC) despite anaplastic features and high grade has a good prognosis that can be related to prominent lymphocytic infiltration. We analyzed the frequency of antibody response toward 41 SEREX (serological recombinant expression cloning)-defined MBC antigens in sera of allogeneic MBC patients using serological plaque-spot assay and examined the mRNA expression profile of some antigens in MBC tissues. This preliminary study allowed us to select 18 autoantigens as potential MBC-associated antigens. Further studies in large cohorts of breast cancer patients will be performed for their evaluation as targets for cancer diagnostics or therapy.
Department of Cell Signaling, Institute of Molecular Biology and Genetics NAS of Ukraine, The State Key Laboratory of Molecular and Cellular Biology, Kyiv, Ukraine. kostjanez@ukr.net
Olga
O
Kostianets
Maksym
M
Shyian
Demidov
D
Sergiy
Stepan
S
Antoniuk
Ivan
I
Gout
Valeriy
V
Filonenko
Ramziya
R
Kiyamova
D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis; D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D015723:Gene Library; D006801:Humans
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor