Evaluation of electrospun (1,3)-(1,6)-β-D-glucans/biodegradable polymer as artificial skin for full-thickness wound healing.
(1,3)-(1,6)-β-D-glucan (BG), a natural product of glucose polymers, has immune stimulatory activity that is especially effective in wound healing. In this study, poly(lactic-co-glycolic acid) (PLGA) membranes containing BGs (BG/PLGA membranes) were investigated for their wound-healing effects. The growth rate of human dermal fibroblasts was enhanced in BG/PLGA membranes. Their growth rates were improved with the increase of BG concentration in the membranes. The PLGA membranes with and without BGs were treated in full-thickness skin wound using male BALB/c nude mice (n=6 for each group). According to the animal study, BG/PLGA membranes enhanced the interaction with the surrounding cells in wound sites. In the wound site treated BG/PLGA, the positive of the Ki-67 (a proliferation cell marker) and the CD 31 (an endothelial cell marker) were 77.2%±5.6% and 34±8.6 capillaries. In the wound site treated PLGA, the Ki-67 positive cells were 51.3%±7.0%, and the positive-stained capillaries of CD 31 were 22.7±8.6. The wound site treated with BG/PLGA membranes was stronger stained of them in the wound site than those of the wound sites treated with PLGA membranes. BG/PLGA membranes accelerated wound healing by improving the interaction, proliferation of cells, and angiogenesis. BG/PLGA membranes can be useful as a skin substitute for enhancing wound healing.
Department of Medical Engineering, Yonsei University College of Medicine, Shinchon-dong, Seodaemun-gu, Seoul, Republic of Korea.
Hye-Lee
HL
Kim
Jeong-Hyun
JH
Lee
Mi Hee
MH
Lee
Byeong Ju
BJ
Kwon
Jong-Chul
JC
Park
D000328:Adult; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001673:Biodegradation, Environmental; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D005936:Glucans / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D019344:Lactic Acid / Q000494:pharmacology*; D008297:Male; D008567:Membranes, Artificial; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing / Q000187:drug effects*
D001672:Biocompatible Materials; D005936:Glucans; D008567:Membranes, Artificial; C033671:epiglucan; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid