Expression of sarcoglycans in the human cerebral cortex: an immunohistochemical and molecular study.
The sarcoglycan (SG) complex (SGC) is a subcomplex within the dystrophin-glycoprotein complex (DGC) and is composed of several transmembrane proteins (α, β, δ, γ, ε and ζ). The DGC supplies a transmembranous connection between the subsarcolemmal cytoskeleton networks and the basal lamina in order to protect the lipid bilayer and to provide a scaffold for signaling molecules in all muscle cells. In addition to its role in muscle tissue, dystrophin and some DGC components are expressed in neurons and glia. Very little is known about the SG subunits in the central nervous system (CNS) and some data suggested the presence of ε and ζ subunits only. In fact, mutations in the ε-SG gene cause myoclonus-dystonia, indicating its importance for brain function. To determine the presence and localization of SGC in the human cerebral cortex, we performed an investigation using immunofluorescence, immunoblotting and reverse transcriptase polymerase chain reaction. The results showed that all SG subunits are expressed in the human cerebral cortex, particularly in large neurons but also in astrocytes. These data suggest that the SG subcomplex may be involved in the organization of CNS synapses.
Department of Biomorphology and Biotechnologies, University of Messina, Messina, Italy.
Giuseppe
G
Anastasi
Francesco
F
Tomasello
Debora
D
Di Mauro
Giuseppina
G
Cutroneo
Angelo
A
Favaloro
Alfredo
A
Conti
Alessia
A
Ruggeri
Carmela
C
Rinaldi
Fabio
F
Trimarchi
D015153:Blotting, Western; D002540:Cerebral Cortex / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D020133:Reverse Transcriptase Polymerase Chain Reaction; D049031:Sarcoglycans / Q000378:metabolism*
D049031:Sarcoglycans