Methyl jasmonate and yeast extract stimulate mitragynine production in Mitragyna speciosa (Roxb.) Korth. shoot culture.
Mitragynine is a pharmacologically-active terpenoid indole alkaloid found in Mitragyna speciosa leaves. Treatment with methyl jasmonate (10 μM) for 24 h and yeast extract (0.1 mg/ml) for 12 h were the optimum conditions of elicitation of mitragynine accumulation in a M. speciosa shoot culture. The former elicitor gave 0.11 mg mitragynine/g dry wt. Tryptophan decarboxylase and strictosidine synthase mRNA levels were enhanced in accordance with mitragynine accumulation.
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand. juraithip.w@psu.ac.th
Juraithip
J
Wungsintaweekul
Jutarat
J
Choo-Malee
Tossaton
T
Charoonratana
Niwat
N
Keawpradub
D000085:Acetates / Q000494:pharmacology*; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000235:genetics / Q000378:metabolism; D019759:Carbon-Nitrogen Lyases / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D046508:Culture Techniques; D003517:Cyclopentanes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D032065:Mitragyna / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D046948:Secologanin Tryptamine Alkaloids / Q000378:metabolism*; D015003:Yeasts / Q000737:chemistry*
D000085:Acetates; D003470:Culture Media; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D012333:RNA, Messenger; D046948:Secologanin Tryptamine Alkaloids; C072239:methyl jasmonate; D001142:Aromatic-L-Amino-Acid Decarboxylases; D019759:Carbon-Nitrogen Lyases; C022684:strictosidine synthetase; C001801:mitragynine