[Experimental study on invigorating kidney and activating blood on preventing and curing SD rats with knee osteoarthritis].
OBJECTIVE
To explore the effects and mechanism of invigorating kidney and activating blood, invigorating kidney and expelling wind on hemorheology, IL-1β and TNF-α of SD rats with knee osteoarthritis, then definite the evolution of muscle certified turning into heumatism and compare the effect of Chinese herbal.


METHODS
One hundred and eighty SD rats with 3-month-old (each weight was 185 to 215 g) received intra-articular injection of papain solution for establishing knee OA models. All rats were randomly divided into activating blood group, preventing group, expelling wind group, invigorating kidney group, invigorating kidney and activating blood group and model group. Laboratory indexes were obtained at the 30th, 60th, 90th days after gastric perfusion, which including state of mind, activity, fur, weight, joint swelling, largely image, hemorheology, inflammation and HE pathological appearance.


RESULTS
After operation, rats appeared blood stasis and swelling and difficulty crawling. There was significant difference of hemorheology in invigorating kidney and activating blood group the content of IL-1β and TNF-α was obviously lower than model group (P < 0.05 ). While the content of IL-1β and TNF-α on the early stage was obviously higher than late stage (P < 0.05).


CONCLUSIONS
Knee osteoarthritis mainly show synovial inflammation at the early stage, inflammation at early stage is more severe than late; invigorating kidney and activating blood decoction can inhibit the knee cartilage injury, improve blood circulation and prevent local inflammatory reaction. Activating blood decoction and invigorating kidney and activating blood Decoction have certain curative effect in early time, but the effects of invigorating kidney and activating blood Decoction is more effective than other on the late stage.
Department of Molecular Pharmacology, Laboratory of Molecular Genetics, Institute of Gubi Syndrome, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China. jiweifeng@zjtcm.net
Wei-Feng
WF
Ji
Wei-Feng
WF
Shi
Lin
L
Chen
Zhen-Chuan
ZC
Ma
Xiao-Feng
XF
Yuan
Jian
J
Xu
Ju
J
Li
Pei-Jian
PJ
Tong
Lu-Wei
LW
Xiao
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D007270:Injections, Intra-Articular; D053583:Interleukin-1beta / Q000276:immunology; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology; D008297:Male; D020370:Osteoarthritis, Knee / Q000188:drug therapy* / Q000276:immunology / Q000503:physiopathology / Q000517:prevention & control; D051381:Rats; D048069:Tumor Necrosis Factors / Q000276:immunology
D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D048069:Tumor Necrosis Factors