Planosporangium thailandense sp. nov., isolated from soil from a Thai hot spring.
A novel filamentous bacterial strain, HSS8-18(T), was isolated from soil from a hot-spring pond in Krabi province, Thailand. The isolate produced single globose bodies and short, finger-like sporangia directly from the substrate mycelium. The cell wall of the isolate contained meso-diaminopimelic acid, glycine, arabinose and xylose. The characteristic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylethanolamine and hydroxyl phosphatidylethanolamine. The predominant menaquinones were MK-10(H2), MK-9(H4) and MK-10(H4) and the predominant cellular fatty acids were anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0, iso-C17 : 0 and anteiso-C15 : 0. The G+C content of the genomic DNA was 72.0 mol%. 16S rRNA gene sequence analysis indicated that isolate HSS8-18(T) belonged to the genus Planosporangium and shared the highest 16S rRNA gene sequence similarity with Planosporangium flavigriseum YIM 46034(T) (98.3 %) and P. mesophilum YIM 48875(T) (98.0 %). However, DNA-DNA hybridization results and some physiological and biochemical properties indicated that strain HSS8-18(T) could be readily distinguished from its closest phylogenetic relatives. Based on phenotypic and genotypic properties, isolate HSS8-18(T) is judged to represent a novel species of the genus Planosporangium, for which the name Planosporangium thailandense sp. nov. is proposed. The type strain is strain HSS8-18(T) ( = BCC 41917(T)  = JCM 17129(T)).
Actinobacterial Research Unit and Microbial Resource Management Unit, Scientific Instrument Center, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.|Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Chitti
C
Thawai
Actinobacterial Research Unit and Microbial Resource Management Unit, Scientific Instrument Center, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Wasinee
W
Thamsathit
Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Takuji
T
Kudo
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D045482:Hot Springs / Q000382:microbiology*; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013785:Thailand; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2