[The effects of exercise and glucose and/or acanthopanacis senticosi after workout on AMPK in muscle cell of rat].
OBJECTIVE
To explore the effect of glucose and/or acanthopanacis senticosi administration supplement on AMP-activated protein kinase (AMPK) activation and its change in different phase after exercise in muscle cell of rat.


METHODS
128 rats were divided into exercise control groups (C groups), exercise and glucose administration groups (G groups), exercise and acanthopanacis senticosi administration groups (A groups), exercise and glucose and acanthopanacis senticosi administration groups (GA groups). The glucose and acanthopanacis senticosi supplement were completed by intragastric administration in half hour after exercise. All rats were killed in different designed time points before or after glycogen depletion exercise (0 h, 4 h and 12 h respectively) and finally divided into 16 groups (n = 8). The values of AMPK in soleus muscle were analyzed by Western blot.


RESULTS
(1) After exercise, the protein content of AMPK quickly increased and reached the peak (209.23 +/- 21.32) then gradually decreased. (2) Acanthopanacis senticosi administration markedly increased the protein content of AMPK in the 0 h and 4 h points after glycogen consumption training (225.11 +/- 20.58 and 186.31 +/- 15.26 vs 195.19 +/- 13.31 and 157.11 +/- 16.43) without any difference after 12 h. (3) Glucose administration had no significant effect on AMPK activation. (4) Both glycogen and acanthopanacis senticosi were supplied simultaneously that had enhanced the AMPK content in 4 h and 12 h point (217.96 +/- 19.25 and 191.86 +/- 14.69). However, the AMPK content in GA group was lower than that in the C groups at 12 h point (121.89 +/- 15.23 vs 137.92 +/- 16.01).


CONCLUSIONS
Exercise could markedly activate the AMPK protein in muscle cell and acanthopanacis senticosi administration augmented such activation. Glucose administration had no significant effect on AMPK activation.
Physicol Education Department of Hunan University of Science and Technology, Xiangtan 411201, China. zhouliang89@sohu.com
Liang
L
Zhou
Ze-Yi
ZY
Yang
D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D028001:Eleutherococcus; D005947:Glucose / Q000494:pharmacology*; D008297:Male; D032342:Muscle Cells / Q000187:drug effects* / Q000201:enzymology*; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D010805:Physical Conditioning, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D055372:AMP-Activated Protein Kinases; D005947:Glucose