[Comparison of different methods for isolating total RNA from bulblet of Fritillaria anhuiensis].
OBJECTIVE
To optimize a simple and effective method for total RNA extraction from bulblet of Fritillaria anhuiensis.


METHODS
Four methods, i. e. guanidine isothiocyanate, bentonite, modified SDS/phenol and the RNAiso plus, were used to extract total RNA from bulblet of F. anhuiensis. Then the results of the extraction were compared and analyzed by electrophoresis detection and RT-PCR verification.


RESULTS
The total RNA extracted by bentonite method were clear and no dispersion, the integrity of the RNA was well, and there was no obvious contamination with DNA and other impurities, was suitable for RT-PCR test.


CONCLUSIONS
The bentonite method is quick, economic, and efficient for total RNA extraction from bulblet of F. anhuiensis.
School of Life Science, Huaibei Normal University, Huaibei, Anhui Key Laboratory of Plant Resources and Biology, Huaibei 235000, China.
Hedong
H
Lu
Tao
T
Xue
Yuezhong
Y
Li
Jianping
J
Xue
Wei
W
Sheng
Yanfang
Y
Zhu
Aimin
A
Zhang
D001546:Bentonite / Q000737:chemistry*; D018076:DNA, Complementary / Q000032:analysis; D004586:Electrophoresis; D031410:Fritillaria / Q000235:genetics*; D006146:Guanidines / Q000737:chemistry; D017879:Isothiocyanates / Q000737:chemistry; D019800:Phenol / Q000737:chemistry; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D018749:RNA, Plant / Q000032:analysis / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013997:Time Factors
D018076:DNA, Complementary; D006146:Guanidines; D017879:Isothiocyanates; D018749:RNA, Plant; C054435:guanidine isothiocyanate; D001546:Bentonite; D019800:Phenol; D012967:Sodium Dodecyl Sulfate