[Simultaneous determination of chlorogenic acid and vitexin 2"-rhamnoside in Crataegi Fructus extracts by HPLC].
OBJECTIVE
To establish a determination method for contents of chlorogenic acid and vitexin 2"-rhamnoside in Crataegi Fructus extracts by HPLC.


METHODS
The chromatographic column was SHISEIDO CAPCELL PAK C18 (4.6 mm x 250 mm, 5 microm); mobile phase was methanol(A) and THF-acetic acid-water (10:2:76, B), with gradient elution, flow speed was 1.0 mL x min(-1); detection wavelength was 330 nm; temperature of column was 25 degrees C.


RESULTS
Chlorogenic acid and vitexin 2"-rhamnoside showed good linear relationships within the ranges of 1.34-164.8 and 1.18-148.7 mg x L(-1), with the recovery rates being 100.4% and 98.8%, and RSD being 1.5% and 1.3% respectively.


CONCLUSIONS
The present method is so simply, accurate and highly repeatable that it can be used to effectively determine the contents of chlorogenic acid and vitexin 2"-rhamnoside in Crataegi Fructus extracts.
School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China. heyajun0228@126.com
Yajun
Y
He
Juan
J
Su
Qian
Q
Yang
Hui
H
Liu
Weidong
W
Zhang
D047310:Apigenin / Q000032:analysis* / Q000302:isolation & purification; D002726:Chlorogenic Acid / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D027825:Crataegus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D002726:Chlorogenic Acid; C021329:vitexin rhamnoside; C007145:crataegus extract; D047310:Apigenin