Mice embryology: a microscopic overview.
In this work, we studied the embryology of mice of 12, 14, and 18 days of gestation by gross observation, light microscopy, and scanning electron microscopy. Grossly, the embryos of 12 days were observed in C-shaped region of the brain, eye pigmentation of the retina, first, second, and third pharyngeal arches gill pit nasal region on the fourth ventricle brain, cervical curvature, heart, liver, limb bud thoracic, spinal cord, tail, umbilical cord, and place of the mesonephric ridge. Microscopically, the liver, cardiovascular system and spinal cord were observed. In the embryo of 14 days, we observed structures that make up the liver and heart. At 18 days of gestation fetuses, it was noted the presence of eyes, mouth, and nose in the cephalic region, chest and pelvic region with the presence of well-developed limbs, umbilical cord, and placenta. Scanning electron microscopy in 18 days of gestation fetuses evidenced head, eyes closed eyelids, nose, vibrissae, forelimb, heart, lung, kidney, liver, small bowel, diaphragm, and part of the spine. The results obtained in this work describe the internal and external morphology of mice, provided by an integration of techniques and review of the morphological knowledge of the embryonic development of this species, as this animal is of great importance to scientific studies.
Department of Surgery, School of Veterinary Medicine and Animal Sciences, University of São Paulo, Brazil. ml.salvadori@usp.br
Maria Letícia Baptista
ML
Salvadori
Thais Borges
TB
Lessa
Fabiele Baldino
FB
Russo
Renata Avancini
RA
Fernandes
José Roberto
JR
Kfoury
Patricia Cristina Baleeiro Beltrão
PC
Braga
Maria Angélica
MA
Miglino
D000825:Animal Structures / Q000033:anatomy & histology / Q000196:embryology* / Q000648:ultrastructure; D000818:Animals; D004626:Embryology / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D009024:Morphogenesis; D013997:Time Factors