Scopoletin attenuates allergy by inhibiting Th2 cytokines production in EL-4 T cells.
Scopoletin is an antioxidant found in certain weedy plants. It exerts anti-inflammatory, anti-allergic, and anti-diabetic activities. It remains unknown whether scopoletin regulates T helper (Th) cells, including Th 1 and Th 2 cells. We found that scopoletin significantly inhibited phorbol myristate acetate (PMA)/ionomycin-induced interleukin-4 (IL-4), IL-5, and IL-10 production in EL-4 T cells. In addition, scopoletin significantly enhanced the inhibitory action of PMA/ionomycin on interferon-γ (IFN-γ) expression. In EL-4 T cells, PMA/ionomycin treatment markedly increased the expression of nuclear factor of activated T cells (NFAT) and GATA-3; in contrast, scopoletin significantly down-regulated expressions of these transcription factors. Furthermore, this downregulation depended on protein kinase C (PKC) attenuation. This leads us to suggest that the anti-allergic properties of scopoletin might be mediated by the downregulation of cytokine expression in Th 2 cells.
Department of Horticulture and Landscape Architecture, National Taiwan University, Roosevelt Road Section 4, Taipei 10617, Taiwan, ROC.
An-Sheng
AS
Cheng
Yu-Hsiang
YH
Cheng
Tsu-Liang
TL
Chang
D000818:Animals; D045744:Cell Line, Tumor; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D006967:Hypersensitivity / Q000517:prevention & control*; D007371:Interferon-gamma / Q000037:antagonists & inhibitors; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D015847:Interleukin-4 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D015759:Ionomycin / Q000494:pharmacology; D016399:Lymphoma, T-Cell; D051379:Mice; D008517:Phytotherapy; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D012603:Scopoletin / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018418:Th2 Cells / Q000187:drug effects* / Q000378:metabolism*
D016207:Cytokines; D007375:Interleukin-1; D016753:Interleukin-10; D015847:Interleukin-4; D015759:Ionomycin; D007371:Interferon-gamma; D011493:Protein Kinase C; D012603:Scopoletin; D013755:Tetradecanoylphorbol Acetate