Ionic liquids dispersive liquid-liquid microextraction and high-performance liquid chromatographic determination of irbesartan and valsartan in human urine.
An environmentally friendly ionic liquids dispersive liquid-liquid microextraction (IL-DLLME) method coupled with high-performance liquid chromatography (HPLC) for the determination of antihypertensive drugs irbesartan and valsartan in human urine samples was developed. The HPLC separations were accomplished in less than 10 min using a reversed-phase C(18) column (250 × 4.60 mm i.d., 5 µm) with a mobile phase containing 0.3 % formic acid solution and methanol (v/v, 3:7; flow rate, 1.0 mL/min). UV absorption responses at 236 nm were linear over a wide concentration range from 50 µg/mL to the detection limits of 3.3 µg/L for valsartan and 1.5 µg/L for irbesartan. The effective parameters on IL-DLLME, such as ionic liquid types and their amounts, disperser solvent types and their volume, pH of the sample and extraction time were studied and optimized. The developed IL-DLLME-HPLC was successfully applied for evaluation of the urine irbesartan and valsartan profile following oral capsules administration.
College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, China.
Zhiling
Z
Li
Fan
F
Chen
Xuedong
X
Wang
Chengjun
C
Wang
D000096:Acetone / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D006801:Humans; D000077405:Irbesartan; D059627:Liquid Phase Microextraction / Q000379:methods*; D015203:Reproducibility of Results; D013777:Tetrazoles / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine*; D013997:Time Factors; D014633:Valine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine; D000068756:Valsartan
D001713:Biphenyl Compounds; D013777:Tetrazoles; D000096:Acetone; D000068756:Valsartan; D014633:Valine; D000077405:Irbesartan