Reduction of antibiotics using microorganisms containing glutathione S-transferases under immobilized conditions.
The degradation of several antibiotics (tetracycline, sulfathiazole, ampicillin) was performed with immobilized bacterial cells containing the glutathione S-transferases (GSTs). Antibiotics in animal feed contaminated wastewater usually inhibit the growth of microorganisms that treat the wastewater, so a bio-friendly treatment method is required. Therefore, we have shown that the inhibitory effects of antibiotics on bacteria were reduced by microorganisms containing detoxifying enzyme GSTs by using a cell immobilizing method in a bioreactor. The initial concentrations of tetracycline, sulfathiazole and ampicillin were 100mg/L, 100mg/L and 50mg/L respectively, which are typical of the range detected in pig feed in Korea. In the results, we observed the removal efficiency of tetracycline to be almost 70% with Staphylococcus epidermidis in the bioreactor, suggesting that this method of antibiotic removal is worthy of further study.
Department of Chemical Engineering, Sungkyunkwan University, 300 Cheoncheondong, Jangangu, Suwon, Gyeonggido 440-746, South Korea. Electronic address: hypark8001@naver.com.
Hyeyoung
H
Park
D000667:Ampicillin / Q000378:metabolism; D000821:Animal Feed; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001644:Bifidobacterium / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D005982:Glutathione Transferase / Q000378:metabolism*; D013212:Staphylococcus epidermidis / Q000378:metabolism*; D000077589:Sulfathiazole; D013432:Sulfathiazoles / Q000378:metabolism; D013752:Tetracycline / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D000667:Ampicillin; D000900:Anti-Bacterial Agents; D005982:Glutathione Transferase; D000077589:Sulfathiazole; D013432:Sulfathiazoles; D013752:Tetracycline; D062065:Wastewater; D014874:Water Pollutants, Chemical