A modified method for inducing periodontitis in dogs using a silk-wire twisted ligature.
This study was designed to assess the effectiveness of a modified silk ligature twisted with wire for inducing advanced periodontitis. Periodontitis was induced in five premolars and one molar of 20 healthy dogs over a 60-day period. The dogs were divided into four groups according to the ligature-inducing materials used: soft moistened food only, wire ligature (WL), silk ligature (SL) and twisted ligature with silk and wire (SWL). Periodontal indices were recorded, and dental radiographs were taken before and after 60 days of ligation. The ligatures were checked daily and the day the ligature fell out was noted. The period during which the ligatures were maintained was significantly shorter for the SL group compared to the SWL group (p < 0.05). Results of the clinical examination showed that almost all periodontal status parameters including the plaque index, gingival index, clinical attachment level, and bleeding on probing were significantly exacerbated in the SWL group compared to the other groups (p < 0.05). Radiographic evaluation demonstrated that alveolar bone levels were significantly lower in the SWL group than the other groups on day 60 (p < 0.05). These results suggested that experimental periodontitis induced by SWL could be an effective method for investigating periodontitis in canine models.
Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Se Eun
SE
Kim
Eui Ri
ER
Lee
Yesran
Y
Lee
Manbok
M
Jeong
Young Woo
YW
Park
Jae Sang
JS
Ahn
Jeong Taek
JT
Ahn
Kangmoon
K
Seo
D016301:Alveolar Bone Loss / Q000662:veterinary; D000701:Analgesics, Opioid / Q000627:therapeutic use; D000818:Animals; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D008026:Ligation / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D008422:Materials Testing / Q000662:veterinary; D010146:Pain / Q000188:drug therapy / Q000662:veterinary; D010518:Periodontitis / Q000473:pathology / Q000662:veterinary*; D014147:Tramadol / Q000627:therapeutic use
D000701:Analgesics, Opioid; D014147:Tramadol