[Research on standardized preparation of traditional Chinese medicine (III): difference of extracting quantity of anthraquinones from mixed decoction of rhubarb with TCMs containing different ingredients].
OBJECTIVE
To detect the influence of compatibility of rhubarb with different traditional Chinese medicines (TCM) on extracted quantities of AQs, and to provide scientific basis for the clinical code for rhubarb preparation.


METHODS
The influence of compatibility of rhubarb with different traditional Chinese medicines (saponin, alkaloids, flavonoids TCMs, animal medicines and mineral medicines) on decocting volume of anthraquinone substance was detected using ultra performance liquid chromatography.


RESULTS
In comparable conditions, more AQs were extracted from mixed decoction of rhubarb and saponin medicinal materials (Astragali Radix, Notoginseng Radix et Rhizoma, Glycyrrhizae Radix et Rhizoma, Polygalae Radix, Ginseng Radix et Rhizoma) than single decocting of rhubarb. The mixed decoction of rhubarb and alkaloid medicinal materials (Coptidis Rhizoma, Sophorae Flavescentis Radix, Prepared Aconiti Lateralis Radix Praeparata, Phellodendri Chinensis Cortex, Aconiti Lateralis Radix) caused a remarkable decrease in extracted quantities of AQs. And the mixed decoction of rhubarb and mineral medicines (Natrii Sulfas, Gypsum Fibrosum, Ostreae Concha, Alumen) also resulted in less extracted quantities of AQs to varying degrees. Besides, more rhubarb AQs were extracted from mixed decoction with Curcuma than single decoction. But less rhubarb AQs were observed in mixed decoction with Lonicerae Flos, Rehmanniae, Artemisiae Herb and Forsythiae Fructus than single decoction to varying degrees. In the study, the maximum extracted quantities of AQs is 2. 3-fold higher than the minimum, the largest difference existed in the extracted quantity of physcion which was 13.5 times.


CONCLUSIONS
In compatibility between rhubarb and different TMCs, mixed decoction and single decoction show different influences on extracted quantity of rhubarb AQs. It is proved that more AQs may be extracted from mixed decoction between rhubarb and saponin medicinal materials, whereas less AQs may be observed in mixed decoction between rhubarb and alkaloid medicinal materials.
China Military Institute of Chinese Materia Medica, 302 Military Hospital, Beijing 100039, China.
Lingna
L
Zeng
Jiabo
J
Wang
Pin
P
Zhang
Yanling
Y
Zhao
Baocai
B
Li
Feifei
F
Liu
Xiaohui
X
Chu
Xiaohe
X
Xiao
D000470:Alkaloids / Q000737:chemistry; D000818:Animals; D000880:Anthraquinones / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004339:Drug Compounding / Q000379:methods* / Q000592:standards; D005419:Flavonoids / Q000737:chemistry; D006801:Humans; D008516:Medicine, Chinese Traditional / Q000379:methods* / Q000592:standards; D010946:Plants, Medicinal / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012250:Rheum / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry
D000470:Alkaloids; D000880:Anthraquinones; D005419:Flavonoids; D012503:Saponins