Constructing spatially separated multienzyme system through bioadhesion-assisted bio-inspired mineralization for efficient carbon dioxide conversion.
A facile and green bioadhesion-assisted bio-inspired mineralization (BABM) approach is proposed to construct spatially separated multienzyme system for conversion of carbon dioxide to formaldehyde. Specifically, formate dehydrogenase is entrapped accompanying the formation of titania nanoparticles (NPs) through bio-inspired titanification. After in situ surface functionalization of NPs with oligodopa, formaldehyde dehydrogenase is immobilized on the surface of NPs through amine-catechol adduct reaction. Compared to co-immobilized and free multienzyme system, the spatially separated multienzyme system exhibits significantly enhanced formaldehyde yield, selectivity and initial specific activity. The influence of particle size on the enzyme activity reveals that the formaldehyde yield (80.9%, 52.9%, 46.4%), selectivity (92.7%, 86.6%, 85.1%) and initial specific activity (1.87, 1.31, 0.29 U mg(-1)) all decreased as the NPs particle size increased from 75, 175 to 375 nm. After storing for 20 days at 4 °C, this multienzyme system retains as high as 70% of its initial activity.
Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
Jiafu
J
Shi
Xiaoli
X
Wang
Zhongyi
Z
Jiang
Yanpeng
Y
Liang
Yuanyuan
Y
Zhu
Chunhong
C
Zhang
D000268:Adhesiveness; D001672:Biocompatible Materials / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism*; D005557:Formaldehyde / Q000378:metabolism; D055772:Green Chemistry Technology; D008903:Minerals / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000302:isolation & purification* / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D056951:Photoelectron Spectroscopy; D059027:Recycling
D001672:Biocompatible Materials; D008903:Minerals; D009097:Multienzyme Complexes; D009243:NAD; D002245:Carbon Dioxide; D005557:Formaldehyde