Matrix metalloproteinase-2 and -9 in the sera and in the urine of human             oncocytoma and renal cell carcinoma.
Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases,             capable of degrading all the molecular components of extracellular matrix. MMPs             have been shown to play critical roles in tumor cell invasion and metastasis.             We verified the activity of MMPs in the sera and in the urine of patients with             kidney carcinoma by gelatin zymography. Of these patients, 16 had clear cell renal             carcinoma (ccRCC) and 4 patients had oncocytoma. The sera and the urine of 16             healthy subjects were used as controls. In the sera, zymography analysis showed             gelatinolytic bands at 72 kDa (gelatinase A) at 92, 130 and 240 kDa (gelatinase B).             MMP-9 activity was slightly enhanced in sera from ccRCC compared with oncocytoma             patients. Serum MMP-2 activity was similar in ccRCC and in oncocytoma patients.             In the urine, 2 oncocytoma patients and 3 (33%) of the ccRCC patients showed gelatinolytic             activity, whereas MMPs could not be detected in the concentrated urine of healthy             subjects. The most abundant lytic activity was at 92 kDa, whereas MMP-2 was present             in lesser quantities. However, there was broad overlap of the data and we did             not find any correlation to type, stage or grade. Therefore, despite previous             evidence, MMP-2 and -9 activity in serum and urine may not be useful biomarker             for kidney carcinomas.
Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, I-00161 Rome, Italy. angelina.dicarlo@uniroma1.it
Angelina
A
Di Carlo
D018249:Adenoma, Oxyphilic / Q000097:blood / Q000201:enzymology* / Q000652:urine; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000652:urine; D002292:Carcinoma, Renal Cell / Q000097:blood / Q000201:enzymology* / Q000652:urine; D016022:Case-Control Studies; D057075:Enzyme Assays; D005260:Female; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood / Q000201:enzymology* / Q000652:urine; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood* / Q000652:urine; D020780:Matrix Metalloproteinase 9 / Q000097:blood* / Q000652:urine; D008875:Middle Aged
D014408:Biomarkers, Tumor; D005780:Gelatin; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9