Hop (Humulus lupulus L.) extract inhibits obesity in mice fed a high-fat diet over the long term.
Hops (Humulus lupulus L.) are traditionally used to add bitterness and flavour to beer. Although the isomerised hop extracts produced by the brewing process have been thought to ameliorate lipid and glucose metabolism, the influence of untreated hop extracts on high-fat (HF) diet-induced obesity is unclear. The present study examined the anti-obesity effects of a hop extract in male C57BL/6J mice fed a HF diet, or HF diet plus 2 or 5 % hop extract for 20 weeks. The oral glucose tolerance test was performed at week 19. Furthermore, water excretion was evaluated in water-loaded Balb/c male mice. The effects of the extract on lipid accumulation and PPARγ expression in 3T3-L1 adipocytes were examined. The hop extract inhibited the increase in body and adipose tissue weight, adipose cell diameter and liver lipids induced by the HF diet. Furthermore, it improved glucose intolerance. The extract enhanced water excretion in water-loaded mice. Various fractions of the hop extract inhibited lipid accumulation and PPARγ expression in 3T3-L1 adipocytes. Hop extracts might be useful for preventing obesity and glucose intolerance caused by a HF diet.
Division of Functional Histology, Department of Functional Biomedicine, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime              791-0295, Japan.
Maho
M
Sumiyoshi
Yoshiyuki
Y
Kimura
D041721:3T3-L1 Cells; D052438:Adipocytes, White / Q000378:metabolism* / Q000473:pathology; D050154:Adiposity; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D048429:Cell Size; D059305:Diet, High-Fat / Q000009:adverse effects; D019587:Dietary Supplements; D005234:Fatty Liver / Q000209:etiology / Q000517:prevention & control; D035263:Flowering Tops / Q000737:chemistry*; D018149:Glucose Intolerance / Q000209:etiology / Q000517:prevention & control; D027582:Humulus / Q000737:chemistry*; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D047495:PPAR gamma / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D014883:Water-Electrolyte Imbalance / Q000178:diet therapy
D019440:Anti-Obesity Agents; D047495:PPAR gamma; D010936:Plant Extracts