Early developmental gene regulation in Strongylocentrotus purpuratus embryos in response to elevated CO₂ seawater conditions.
Ocean acidification, or the increased uptake of CO(2) by the ocean due to elevated atmospheric CO(2) concentrations, may variably impact marine early life history stages, as they may be especially susceptible to changes in ocean chemistry. Investigating the regulatory mechanisms of early development in an environmental context, or ecological development, will contribute to increased understanding of potential organismal responses to such rapid, large-scale environmental changes. We examined transcript-level responses to elevated seawater CO(2) during gastrulation and the initiation of spiculogenesis, two crucial developmental processes in the purple sea urchin, Strongylocentrotus purpuratus. Embryos were reared at the current, accepted oceanic CO(2) concentration of 380 microatmospheres (μatm), and at the elevated levels of 1000 and 1350 μatm, simulating predictions for oceans and upwelling regions, respectively. The seven genes of interest comprised a subset of pathways in the primary mesenchyme cell gene regulatory network (PMC GRN) shown to be necessary for the regulation and execution of gastrulation and spiculogenesis. Of the seven genes, qPCR analysis indicated that elevated CO(2) concentrations only had a significant but subtle effect on two genes, one important for early embryo patterning, Wnt8, and the other an integral component in spiculogenesis and biomineralization, SM30b. Protein levels of another spicule matrix component, SM50, demonstrated significant variable responses to elevated CO(2). These data link the regulation of crucial early developmental processes with the environment that these embryos would be developing within, situating the study of organismal responses to ocean acidification in a developmental context.
Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA 93106-9620, USA. lmh75@georgetown.edu
LaTisha M
LM
Hammond
Gretchen E
GE
Hofmann
D000468:Alkalies; D000818:Animals; D036703:Blastula / Q000187:drug effects / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology*; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism*; D005775:Gastrula / Q000187:drug effects / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D012623:Seawater / Q000737:chemistry*; D047270:Strongylocentrotus purpuratus / Q000196:embryology* / Q000235:genetics*
D000468:Alkalies; D002245:Carbon Dioxide