An organic/inorganic hybrid membrane as a solid "turn-on" fluorescent chemosensor for coenzyme A (CoA), cysteine (Cys), and glutathione (GSH) in aqueous media.
The preparation of a fluorogenic sensory material for the detection of biomolecules is described. Strategic functionalisation and copolymerisation of a water insoluble organic sensory molecule with hydrophilic comonomers yielded a crosslinked, water-swellable, easy-to-manipulate solid system for water "dip-in" fluorogenic coenzyme A, cysteine, and glutathione detection by means of host-guest interactions. The sensory material was a membrane with gel-like behaviour, which exhibits a change in fluorescence behaviour upon swelling with a water solution of the target molecules. The membrane follows a "turn-on" pattern, which permits the titration of the abovementioned biomolecules. In this way, the water insoluble sensing motif can be exploited in aqueous media. The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II). The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane.
Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Burgos, Spain. svallejos@ubu.es
Saúl
S
Vallejos
Pedro
P
Estévez
Saturnino
S
Ibeas
Félix C
FC
García
Felipe
F
Serna
José M
JM
García
D003065:Coenzyme A / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D010879:Piperazines / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry
D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D010879:Piperazines; D014867:Water; C003905:3,6-bis(5-chloro-2-piperidyl)-2,5-piperazinedione; D005978:Glutathione; D003545:Cysteine; D003065:Coenzyme A