[Fingerprint of Angelica polymorpha by HPLC].
OBJECTIVE
To establish the HPLC fingerprint of Angelica polymorpha.


METHODS
The 10 batches of A. polymorpha were measured by HPLC with isoimperatorin as a reference substance and the chromatographic experients were performed on Kromasil 100A C18 column (4.6 mm x 250 mm, 5 microm), eluted with acetonitrile and water as mobile phase in gradient mode. The flow rate was 1.0 m x min(-1) and the detection wavelength was 254 nm.


RESULTS
The common mode of the HPLC fingerprints were set up. There were 8 common peaks in the fingerprint of 10 samples, and the similarity of the 10 samples was more than 0.9.


CONCLUSIONS
The method is simple, accurate and have a good reaptability. The quality of A. polymorpha can be controlled effectively by the HPLC fingerprint.
Hubei University of Traditional Chinese Medicine, Wuhan 430065, China. ljq59169@sohu.com
Jinqing
J
Lu
Qin
Q
Wang
Jiaqi
J
Xu
Dongli
D
He
Xiaoyun
X
Ruan
Xiaoyan
X
Hu
D029969:Angelica / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D011564:Furocoumarins / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; C055542:isoimperatorin