Development of Exon-Primed Intron-Crossing (EPIC) PCR primers for the malaria vector Anopheles pseudopunctipennis (Diptera: Culicidae).
Using the Anopheles gambiae Giles genome as a template, we designed, screened and identified 14 novel Exon-Primed Intron-Crossing (EPIC) PCR primer pairs for Anopheles pseudopunctipennis Theobald 1901, a major vector of human Plasmodium sp. in South America. These primers were designed to target the conserved regions flanking consecutive exons of different genes and enabled the amplification of 17 loci of which nine were polymorphic. Polymorphisms at these loci ranged from two to four alleles. Intron length polymorphism analysis is a useful tool, which will allow the study of the population structure of this mosquito species, which remains poorly understood.
Institut de recherche pour le développement (IRD), C.P. 9214 La Paz, Bolivia. frederic.lardeux@ird.fr
Frédéric
F
Lardeux
Claudia
C
Aliaga
Rosenka
R
Tejerina
Raùl
R
Ursic-Bedoya
D000483:Alleles; D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001838:Bolivia; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics*; D005091:Exons; D005260:Female; D007303:Insect Vectors / Q000235:genetics*; D007438:Introns; D016778:Malaria, Falciparum / Q000635:transmission; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D013045:Species Specificity
D017931:DNA Primers