Hydrogen saline is protective for acute lung ischaemia/reperfusion injuries in rats.
BACKGROUND
Protective effects of saturated hydrogen (H(2)) saline on cardiac ischaemia-reperfusion (I/R) injury have been demonstrated previously. This study was designed to show that hydrogen-rich saline is protective in preventing lung I/R injury in rats.


METHODS
Adult male Sprague-Dawley rats underwent 45 min occlusion of the right lung roots and 120 min reperfusion. Rats were divided randomly into three groups: sham-operated control group, I/R plus saline treatment, and I/R plus hydrogen-rich saline treatment (0.6 mmol/L, 0.5 ml/kg/d). Three days of intraperitoneal injection of hydrogen-rich saline before the reperfusion combined with immediate administration of hydrogen-rich saline after the reperfusion were performed. Following reperfusion, the lung tissue and the pulmonary artery was immediately obtained and the W/D ratio, pulmonary artery contraction and relaxation ability, H-E staining, TUNEL staining, caspase-3, MDA, 8-OHdG content and measurement of such biomarkers as WBC, CRP were measured or carried out.


RESULTS
Hydrogen saline significantly protected vasoactivity of the pulmonary artery, reduced pulmonary oedema, decreased lung malondialdehyde (MDA), 8-OHdG concentration, alleviated lung epithelial cell apoptosis and lowered the level of such biomarkers as WBC, CRP, ALT and TBiL.


CONCLUSIONS
It is concluded that hydrogen-rich saline is a novel, simple, safe and effective method to attenuate pulmonary I/R injury.
Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Jianxin
J
Shi
Feng
F
Yao
Chenxi
C
Zhong
Xufeng
X
Pan
Yu
Y
Yang
Qiang
Q
Lin
D000080242:8-Hydroxy-2'-Deoxyguanosine; D055371:Acute Lung Injury / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D000818:Animals; D015415:Biomarkers / Q000097:blood; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D006859:Hydrogen / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D011651:Pulmonary Artery / Q000378:metabolism / Q000503:physiopathology; D011654:Pulmonary Edema / Q000097:blood / Q000188:drug therapy / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D020545:Respiratory Mucosa / Q000378:metabolism / Q000503:physiopathology; D012965:Sodium Chloride / Q000493:pharmacokinetics*
D015415:Biomarkers; D012965:Sodium Chloride; D008315:Malondialdehyde; D006859:Hydrogen; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine