[PPARgamma agonists against respiratory syncytial virus infection in vitro study].
OBJECTIVE
To search the effect of PPARgamma agonists for infection of RSV in vitro.


METHODS
The CPE of Hep-2 and A549 cells induced by RSV infection were observed. The effects of 15d-PGJ2 and rosiglitazone on change of CPE of A549 cells induced by RSV infection for 48 h were observed, too. MTT assay was used to detect the rate of viral suppression, and the protective effects of 15d-PGJ2 and rosiglitazone on A549 cells induced by RSV infection for 48 h.


RESULTS
A549 cells interfered by 15d-PGJ2 (5 -25 micromol/L) and rosiglitazone (10-50 micromol/L) did not show obvious CPE, MTT assay also showed that the survival rate of A549 cells induced by RSV infection with PPARgamma agonists added, was significantly higher than that of RSV infection without PPARgamma agonists added, the difference was statistically significant (P < 0.01), but comparision between the two drugs showed no statistical significance. The optimal concentrations of 15d-PGJ2 and rosiglitazone were 5 micromol/L and 10 micromol/L respectively.


CONCLUSIONS
PPARgamma agonist can reduce the CPE of A549 cells after RSV infection and improve the survival rate of A549 cells. PPARgamma agonist can counteract the infection of RSV in A549 cells.
Wenzhou Medical College, Yuying Children's Hospital, Department of Respiratory Medicine, Wenzhou 325027, China.
Chun-Jie
CJ
Wan
Lin
L
Dong
Jie
J
Lin
Xiao-Fang
XF
Chen
D002478:Cells, Cultured; D006801:Humans; D047495:PPAR gamma / Q000819:agonists*; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000494:pharmacology; D012136:Respiratory Syncytial Viruses / Q000187:drug effects*; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology*
C477819:15-deoxyprostaglandin J2; D047495:PPAR gamma; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D015230:Prostaglandin D2