Conversion of estrone to 17-beta-estradiol in human non-small-cell lung cancer cells in vitro.
It has recently been suggested that, in addition to genetic and environmental factors and tobacco exposure, estrogens also may be an independent risk factor in the development of lung cancer. Therefore, we evaluated the transcript and protein levels of 17-beta-hydroxysteroid-dehydrogenase type 1 (HSD17B1), and the conversion of estrone (E1) to 17-beta-estradiol (E2) in human non-small cell lung cancer (NSCLC) Calu-6, Calu-1 and A549 cells. In our work, we established the presence of HSD17B1 transcripts and proteins in all examined NSCLC cells. Moreover, we demonstrated that human NSCLC Calu-6, Calu-1 and A549 cells are able to convert weak estrogen E1 to highly potent E2 in vitro. Our results indicate that NSCLC cells are able to produce E2 from E1.
Poznań University of Medical Sciences, Department of Biochemistry and Molecular Biology, Poznań, Poland.
Hanna
H
Drzewiecka
Paweł P
PP
Jagodzinski
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045744:Cell Line, Tumor; D004958:Estradiol / Q000378:metabolism*; D004970:Estrone / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D012307:Risk Factors
D004970:Estrone; D004958:Estradiol; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase