Molecular organization of small plasmids bearing blaTEM-1 and conferring resistance to β-lactams in Haemophilus influenzae.
TEM-1 is the dominant β-lactamase of Haemophilus influenzae and can be located on small plasmids. Three distinct plasmids with sizes from 4,304 to 5,646 nucleotides (nt) were characterized: pA1606, pA1209, and pPN223. In addition to TEM-1 and a replication enzyme of the Rep 3 superfamily, pA1606 carries a Tn3 resolvase gene and pA1606 and pA1209 carry an open reading frame (ORF) similar to a plasmid recombination enzyme gene described in Gram-positive bacteria. The plasmids transformed strain Rd to the ampicillin-resistant phenotype.
Department of Clinical Microbiology, Aarhus University Hospital, Aarhus, Denmark.
Annette
A
Søndergaard
Alvaro
A
San Millan
Alfonso
A
Santos-Lopez
Signe M
SM
Nielsen
Bruno
B
Gonzalez-Zorn
Niels
N
Nørskov-Lauritsen
D000667:Ampicillin / Q000494:pharmacology; D001483:Base Sequence; D006193:Haemophilus influenzae / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D045522:Recombinases / Q000235:genetics; D014169:Transformation, Bacterial; D045564:Transposon Resolvases / Q000235:genetics; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
D045522:Recombinases; D047090:beta-Lactams; D000667:Ampicillin; C473491:Tn3 resolvase; D045564:Transposon Resolvases; D012324:RNA-Dependent RNA Polymerase; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1