Heat shock treatment improves Trametes versicolor laccase production.
An efficient heat shock strategy has been developed to improve laccase production in submerged Trametes versicolor cultures. The optimized heat shock strategy consists of subjecting T. versicolor mycelial pellets to three heat shock treatments at 45 °C for 45 min, starting at culture day 0, with a 24-h interval between treatments. Laccase production increased by more than 1.6-fold relative to the control in both flasks and a 5-L bioreactor because the expression of the laccase gene was enhanced by heat shock induction. The present work demonstrates that heat shock induction is a promising method because it both improves fungal laccase production and has a good potential in industrial application.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
Feng
F
Wang
Chen
C
Guo
Tao
T
Wei
Tian
T
Zhang
Chun-Zhao
CZ
Liu
D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D046508:Culture Techniques; D015966:Gene Expression Regulation, Fungal; D018869:Heat-Shock Response; D007101:Immersion; D042845:Laccase / Q000096:biosynthesis* / Q000235:genetics; D013997:Time Factors; D055454:Trametes / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology
D042845:Laccase