Adapter-modified Ussing chamber enables evaluation of endoscopically-obtained colonic biopsy samples from cats and dogs.
Adapter-modified Ussing chambers have been used for assessment of endoscopically obtained intestinal biopsies in humans. The aim of this study was to evaluate the feasibility of an adapter-modified Ussing chamber for assessment of intestinal transport physiology in endoscopically-obtained colonic biopsies from cats and dogs. Fifteen colonic biopsies from four cats and 13 colonic biopsies from four dogs were transferred into a modified Ussing chamber and sequentially exposed to several compounds. Baseline mean±SD conductance was measured. Changes of short circuit current (ΔIsc) were observed after exposure to glucose (number of feline biopsies that responded=0/number of canine biopsies that responded=4), phloridzin (n=0/n=7), histamine (n=5/n=12), serotonin (n=7/n=12), prostaglandin (n=5/n=7), forskolin (n=7/n=7), and ouabain (n=9/n=7). The adapter-modified Ussing chamber studied here enables investigation of transport physiology of endoscopically-obtained colonic biopsies from companion animals. However, we observed a large variability of results, suggesting that clinical use of this method is limited.
Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4474, USA. isabelle.ruhnke@fu-berlin.de
Isabelle
I
Ruhnke
John V
JV
DeBiasio
Jan S
JS
Suchodolski
Shelley J
SJ
Newman
Mark W
MW
Musch
Jörg M
JM
Steiner
D000818:Animals; D001706:Biopsy / Q000379:methods / Q000662:veterinary; D002371:Cat Diseases / Q000473:pathology*; D002415:Cats; D005576:Colforsin; D003108:Colonic Diseases / Q000473:pathology / Q000662:veterinary*; D003113:Colonoscopy / Q000662:veterinary*; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D004553:Electric Conductivity; D005260:Female; D005947:Glucose; D006632:Histamine; D008297:Male; D010695:Phlorhizin; D012701:Serotonin; D013048:Specimen Handling / Q000295:instrumentation / Q000662:veterinary*
D005576:Colforsin; D012701:Serotonin; D006632:Histamine; D010695:Phlorhizin; D005947:Glucose