Characterisation and differentiation potential of bone marrow derived canine mesenchymal stem cells.
Mesenchymal stem cells (MSCs) have potential for use in regenerative therapeutics, since they are capable of multi-lineage differentiation. In this study, primary canine MSCs (cMSCs) were isolated from bone marrow aspirates and characterised using marker expression and morphology. cMSCs expressed CD44 and STRO-1, but not CD34 or CD45. Morphologically, cMSCs were similar to previously described MSCs and were capable of chondrocyte differentiation towards articular type cartilage, characterised by increased collagen type II vs. collagen type I expression and expression of Sox-9. cMSCs demonstrated no significant alterations in marker profiles and failed to differentiate into cardiomyocytes in response to a cardiac differentiation protocol or when co-cultured with canine cardiac stem cells. The study indicated that cMSCs can be derived readily from bone marrow and are capable of differentiation into articular cartilage, but appear to have limited ability to differentiate into cardiomyocytes using current protocols.
Royal (Dick) School of Veterinary Studies and The Roslin Institute, The University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK. Hannah.Geere@ed.ac.uk
Hannah M
HM
Hodgkiss-Geere
David J
DJ
Argyle
Brendan M
BM
Corcoran
Bruce
B
Whitelaw
Elspeth
E
Milne
David
D
Bennett
Sally A
SA
Argyle
D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D020219:Chondrogenesis; D018920:Coculture Techniques; D004285:Dogs; D005260:Female; D005786:Gene Expression Regulation; D005819:Genetic Markers; D007150:Immunohistochemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D016133:Polymerase Chain Reaction
D000954:Antigens, Surface; D015415:Biomarkers; D005819:Genetic Markers