Early changes in the synapses of the neostriatum induced by perinatal asphyxia.
Perinatal asphyxia (PA) is a medical condition associated with a high short-term morbimortality and different long-term neurological diseases. In previous work we have observed at 6 months post-synaptic densities (PSDs) alterations compatible with neurodegeneration highly correlated  with the increment in the ubiquitination. Although alterations in the synaptic organization and function have been related with neuronal death after hypoxia, little is known about the synaptic changes in young animals exposed to PA. The main aim of this work is to study the PSDs changes in  striatum of 30-day-old rats subjected to PA. Using two-dimensional electron microscopic analyses of synapses staining with ethanolic phosphotungstic acid we observed an increment of PSD thickness in severe hypoxic rats. These data are consistent with the western blot analysis that showed an  increment in ubiquitination levels in the synapses of severe hypoxic rat. We did observe any alterations neither in synaptic structure nor in ubiquitinization in mild asphyctic rats. These data suggest that hypoxia might cause early misfolding and aggregation of synaptic proteins in severe  anoxic animas that could induce long-term neurodegeneration.
Universidad Argentina John F Kennedy, Buenos Aires, Argentina.
M
M
Grimaldi
I
I
Romer
M T González
MT
de Apodaca
L
L
Iturbe
I D
ID
Catania
J
J
González
R
R
Kolliker-Fres
G
G
Barreto
F
F
Capani
D000818:Animals; D001237:Asphyxia / Q000473:pathology*; D015153:Blotting, Western; D003342:Corpus Striatum / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D017072:Neostriatum / Q000473:pathology*; D018743:Perinatal Care / Q000379:methods*; D057907:Post-Synaptic Density / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000473:pathology*; D014452:Ubiquitins / Q000378:metabolism
D014452:Ubiquitins