Chemically-defined scaffolds created with electrospun synthetic nanofibers to maintain mouse embryonic stem cell culture under feeder-free conditions.
Embryonic stem cells (ESCs) are useful resources for drug discovery, developmental biology and disease studies. Cellular microenvironmental cues play critical roles in regulating ESC functions, but it is challenging to control them with synthetic components. Nanofibers hold a potential to create artificial cellular cues for controlling cell adhesion and cell-cell interactions. Mouse ESC (mESC) were cultured on electrospun nanofibers made from polymethylglutarimide (PMGI), which is a synthetic thermoplastic polymer stable under culture conditions. Both topology and the density of PMGI nanofibers were key factors. mESCs on nanofibers had a growth rate comparable to those cultured conventionally and retained their pluripotency. Furthermore, self-renewed ESCs differentiated into all three germ layers thereby providing a reliable way to expand mESCs without feeder cells.
Institute for Integrated Cell-Material Sciences, WPI-iCeMS, Kyoto University, Kyoto 606-8501, Japan.
Li
L
Liu
Qinghua
Q
Yuan
Jian
J
Shi
Xin
X
Li
Dongju
D
Jung
Li
L
Wang
Kaori
K
Yamauchi
Norio
N
Nakatsuji
Ken-ichiro
K
Kamei
Yong
Y
Chen
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D003470:Culture Media; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D007094:Imides / Q000737:chemistry; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry*
D003470:Culture Media; D007094:Imides; D011108:Polymers