Quantum dot-enhanced detection of dual short RNA sequences via one-step template-dependent surface hybridization.
A novel multiplexed method for short RNA detection is reported that employs a design strategy in which capture and reporter probes anneal to each other in the presence of a short RNA target via the formation of a stable three-component complex. Quantum dots (QDs) functionalized with reporter DNA are thus specifically bound onto a capture probe-modified 96-well plate by one-step hybridization for simple RNA detection. In comparison with conventional organic dye-modified reporter probes, the use of reporter DNA-modified QD conjugates increase the melting temperature and lead to the detection of short RNA without the need for a ligation reaction. Moreover, QD properties allow multiple short RNA sequences to be simultaneously determined via rapid and simple one-step hybridization, as exemplified herein. The present results clearly demonstrate that this new strategy can be used to detect dual-short RNA sequence at concentrations of 10 pM in 100 μL.
School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education & PLA, Fudan University, 826 Zhangheng Road, Shanghai 201203, China.
Wenqing
W
Song
Xue
X
Qiu
Choiwan
C
Lau
Jianzhong
J
Lu
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D045663:Quantum Dots; D012313:RNA / Q000032:analysis* / Q000235:genetics; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D012313:RNA; D004247:DNA