The increased expression of CD11c and CD103 molecules in the neutrophils of the peripheral blood treated with a formula of bacterial ribosomes and proteoglycans of Klebsiella pneumoniae.
OBJECTIVE
To evaluate the effect of a preparation with bacterial ribosomes and proteoglycans from Klebsiella pneumoniae «R» on the in vitro expression of CD11c and CD103 molecules in neutrophils from peripheral blood.


METHODS
Isolation of neutrophils from peripheral blood with Ficoll-Paque, incubation with R and detection of CD11c and CD103 through flow cytometry.


RESULTS
Six hours after the incubation period, CD11c expression increased significantly compared with the control with 125 and 500μg/ml of R (P=.017 and P=.006, respectively). CD103 expression induced with 125μg/ml of R after 6hours was significantly higher than that observed after 4hours at the same concentration (P=.014) and that found with 62.5μg/ml (P=.017) of R.


CONCLUSIONS
The increased expression of CD11c and CD103 induced by R in the neutrophils could contribute to the R mechanism against respiratory pathogens.
Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México.
Jessica
J
Villa-Ambriz
Alain R
AR
Rodríguez-Orozco
Carlos
C
Béjar-Lozano
Christian
C
Cortés-Rojo
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000942:Antigens, Bacterial / Q000494:pharmacology*; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D001773:Blood Cells / Q000187:drug effects / Q000378:metabolism; D039521:CD11c Antigen / Q000096:biosynthesis* / Q000235:genetics; D002448:Cell Adhesion; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D039001:Integrin alpha Chains / Q000096:biosynthesis* / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000737:chemistry; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011509:Proteoglycans / Q000302:isolation & purification / Q000494:pharmacology; D012270:Ribosomes
D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D015703:Antigens, CD; D039521:CD11c Antigen; D039001:Integrin alpha Chains; D011509:Proteoglycans; C034206:Ribomunyl; C091367:alpha E integrins