Fluorescent banding pattern and species-specific DNA marker in Rumex thyrsiflorus Fingerh.
The object of this work was to analyze the karyotype structure of Rumex thyrsiflorus using differential fluorescent methods of chromosome staining (C-banding/DAPI and CMA3/DA/DAPI) and molecular sex markers. The results obtained were compared with data on the structure of the sex chromosomes and autosomes in R. acetosa, a model species in studies of sex determination and sex chromosome evolution in plants with an XX/XY1Y2 system. A high level of similarity was found in the sex chromosome structure of the 2 species, along with small differences in their autosomal complexes. It suggests that differentiation of these 2 closely related species was not accompanied by major structural changes within their sex chromosomes. Molecular tests, however, revealed differences in the composition of male-specific repetitive sequence RAYSII, occurring in the Y1 chromosome. Amplification of this sequence showed the presence of a single product (∼700 bp) in R. acetosa and of 2 products (∼600 bp and ∼700 bp) in R. thyrsiflorus. The longer product (∼700 bp) was also revealed in R. arifolius, another species closely related to R. acetosa. The shorter DNA fragment, characteristic of R. thyrsiflorus, differed from the common product by of a large indel with a length of 110 bp. This fragment may serve as a species-specific molecular marker useful in taxonomical and population studies as well as in further research on the sex chromosome differentiation in R. thyrsiflorus.
Department of Plant Breeding and Seed Science, Cytogenetics Group, University of Agriculture in Cracow, Cracow, Poland. rrjoachi@cyf-kr.edu.pl
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A
Grabowska-Joachimiak
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D
Kwolek
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Kula
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Marciniuk
D001483:Base Sequence; D002871:Chromosome Banding / Q000379:methods*; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D005453:Fluorescence; D005819:Genetic Markers; D007621:Karyotyping; D016133:Polymerase Chain Reaction; D031804:Rumex / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers