Alumina nanoparticles-induced effects on wastewater nitrogen and phosphorus removal after short-term and long-term exposure.
Alumina nanoparticles (Al₂O₃ NPs) have been widely used in many fields, which causes a growing concern about their potential health and environmental risks. However, their possible impacts on wastewater nitrogen and phosphorus removal have not yet been reported. In this study, both short-term and long-term effects of Al₂O₃ NPs on wastewater nutrient removal were investigated. Scanning electron microscope (SEM) analysis showed that most of Al₂O₃ NPs were adsorbed onto activated sludge, but these NPs had no adverse effects on the surface integrity and viability of activated sludge. It was found that short-term exposure to 1 and 50 mg/L Al₂O₃ NPs induced marginal influences on wastewater nitrification, denitrification and phosphorus removal. Nevertheless, the prolonged exposure to 50 mg/L Al₂O₃ NPs was observed to decrease the total nitrogen (TN) removal efficiency from 80.4% to 62.5% due to the suppressed denitrification process, although biological phosphorus removal and the transformations of intracellular polyhydroxyalkanoates and glycogen were not affected. Quantitative PCR assays indicated that compared with the control, 50 mg/L Al₂O₃ NPs decreased the abundance of denitrifying bacteria in activated sludge. Further enzyme activity tests showed that the activities of key denitrifying enzymes (nitrate reductase and nitrite reductase) were inhibited, which might be responsible for the negative effects of 50 mg/L Al₂O₃ NPs on wastewater nitrogen removal after long-term exposure.
State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Yinguang
Y
Chen
Yinglong
Y
Su
Xiong
X
Zheng
Hong
H
Chen
Hong
H
Yang
D000327:Adsorption; D000537:Aluminum Oxide / Q000737:chemistry*; D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D058440:Denitrification; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009584:Nitrogen / Q000302:isolation & purification*; D010758:Phosphorus / Q000302:isolation & purification*; D012722:Sewage / Q000737:chemistry / Q000382:microbiology; D013997:Time Factors; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D018508:Water Purification / Q000379:methods*
D012722:Sewage; D014874:Water Pollutants, Chemical; D010758:Phosphorus; D007770:L-Lactate Dehydrogenase; D000537:Aluminum Oxide; D009584:Nitrogen