Injectable in situ physically and chemically crosslinkable gellan hydrogel.
An injectable, in situ physically and chemically crosslinkable gellan hydrogel is synthesized via gellan thiolation. The thiolation does not alter the gellan's unique 3-D conformation, but leads to a lower phase transition temperature under physiological conditions and stable chemical crosslinking. The synthesis and hydrogels are characterized by (1)H NMR, FT-IR, CD, or rheology measurements. The injectability and the tissue culture cell viability is also tested. The thiolated gellan hydrogel exhibits merits, such as ease for injection, quick gelation, lower gelling temperature, stable structure, and nontoxicity, which make it promising in biomedicine and bioengineering as an injectable hydrogel.
Department of Surgery and Medical Research, Veteran Affairs Medical Center, St. Louis, MO 63106, USA. hdu0228@gmail.com
Hongwei
H
Du
Paul
P
Hamilton
Mattew
M
Reilly
Nathan
N
Ravi
D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D003432:Cross-Linking Reagents / Q000737:chemistry; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D020100:Hydrogels; D007267:Injections / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D044367:Phase Transition; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000187:drug effects; D012212:Rheology; D017550:Spectroscopy, Fourier Transform Infrared; D013439:Sulfhydryl Reagents / Q000737:chemistry; D013696:Temperature
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020100:Hydrogels; D011135:Polysaccharides, Bacterial; D013439:Sulfhydryl Reagents; C048288:gellan gum