New alkyl-phosphate bonded stationary phases for liquid chromatographic separation of biologically active compounds.
A new type of bonded stationary phase for liquid chromatography, with the properties of immobilized artificial membranes, has been synthesized. Alkyl-phosphate adsorbents were obtained by modification of aminopropyl silica gel. The structures of the synthesized materials were confirmed by use of instrumental techniques--elemental analysis, infrared spectroscopy (FTIR), and (13)C and (29)Si CP/MAS NMR. Analysis revealed that the adsorbents mimic the phospholipids present in natural cell membranes. The new synthesized alkyl-phosphate stationary phases may be used for liquid chromatographic separation of biologically active compounds of different polarity.
Department of Environmental Chemistry and Bioanalysis, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.
Szymon
S
Bocian
Alicja
A
Nowaczyk
Boguslaw
B
Buszewski
D000319:Adrenergic beta-Antagonists / Q000737:chemistry; D000327:Adsorption; D001688:Biological Products / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D008567:Membranes, Artificial; D010755:Organophosphates / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared
D000319:Adrenergic beta-Antagonists; D001688:Biological Products; D008567:Membranes, Artificial; D010755:Organophosphates; D010743:Phospholipids; C021611:aminopropyl silica gel; D012822:Silicon Dioxide