Osteocompatibility evaluation of poly(glycine ethyl ester-co-alanine ethyl ester)phosphazene with honeycomb-patterned surface topography.
Biodegradable amino acid ester-substituted polyphosphazenes are unique biomaterials for tissue engineering. Considering the surface properties as topography and chemical composition having vital roles in regulating cellular response, in this study, a kind of micropatterned polyphosphazene films were prepared and subjected to osteoblasts culture. Briefly, poly(glycine ethyl ester-co-alanine ethyl ester)phosphazene (PGAP) was synthesized, and its solution in chloroform was cast under high (80%) or low (20%) environmental humidity. Honeycomb-patterned or flat PGAP films were resulted. By analyzing with scanning electron microscope, atomic force microscope, X-ray photoelectron spectroscope, and water contact angle measurement, the honeycomb-patterned PGAP films demonstrated higher surface roughness, phosphorous and nitrogen content, and hydrophilicity than the flat one. Although the initial cell attachment and proliferation on PGAP films were inferior to those on conventional poly(lactic-co-glycolic acid) films, P-containing PGAP was a sort of bone-binding bioactive polymer. With these alternations, honeycomb-patterned PGAP films had accordingly enhanced protein adsorption and apatite deposition in simulated body fluid and showed great advantages in promoting osteogenous differentiation. The results suggested a potential way to make polyphosphazenes as good choices for bone tissue regeneration by increasing their surface roughness and phosphorous content.
State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, North 3rd Ring Road, Chaoyang District, Beijing 100029, People's Republic of China.
Shun
S
Duan
Xiaoping
X
Yang
Jifu
J
Mao
Bing
B
Qi
Qing
Q
Cai
Hong
H
Shen
Fei
F
Yang
Xuliang
X
Deng
Shenguo
S
Wang
D000042:Absorption; D000818:Animals; D001031:Apatites / Q000378:metabolism; D001842:Bone and Bones / Q000187:drug effects*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D018360:Crystallography, X-Ray; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D019344:Lactic Acid / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D011100:Polyglycolic Acid / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013499:Surface Properties
D001031:Apatites; D008903:Minerals; D009943:Organophosphorus Compounds; D010455:Peptides; D011108:Polymers; C581240:poly(glycine ethyl ester-co-alanine ethyl ester)phosphazene; C108974:poly(phosphazene); D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid