Phenotypic and genotypic characterization of Thai oral streptococci, lactobacilli and pediococci.
This work was to assess the isolation and identification of Thai oral bacteria.   Seventeen viridans group streptococci (VGS) and seventeen lactic acid bacteria from saliva   were characterization based on their morphological, physiological and biochemical   characteristics and the 16S RNA gene sequences analysis. The Gram-positive cocci in chains   of viridans group comprised of six isolates (Group I) were identified as Streptococcus gordonii,   6 isolates (Group II) were Streptococcus salivarius, 4 isolates (Group III) were Streptococcus   sanguis and one isolate (Group IV) was Streptococcus mutans. Seventeen lactic acid bacterial   isolates were Gram-positive and catalase negative. They did not contained diaminopimelic   acid in the cell wall peptidoglycan. They were divided into four groups, the rod-shaped in   Group A (11 isolates) and Group B (2 isolates) which were identified as Lactobacillus   fermentum and Lactobacillus salivarius, respectively. The tetrad-forming cocci in Group C   (3 isolates) and Group D (1 isolate) were identified as Pediococcus acidilactici and P.   pentosaceus, respectively.
Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand.
J
J
Kuvatanasuchati
N
N
Chamroensaksri
S
S
Tanasupawat
D015373:Bacterial Typing Techniques; D002374:Catalase / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry; D003960:Diaminopimelic Acid / Q000737:chemistry; D020459:Genes, rRNA; D005838:Genotype; D005840:Gentian Violet; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D007778:Lactobacillus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009055:Mouth / Q000382:microbiology; D010376:Pediococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010619:Phenazines; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D012463:Saliva / Q000382:microbiology*; D013291:Streptococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D013785:Thailand / Q000453:epidemiology
C035863:Gram's stain; D010457:Peptidoglycan; D010619:Phenazines; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid; D002374:Catalase; D005840:Gentian Violet