Trauma-specific insults to the cochlear nucleus in the rat.
The effect of acoustic overstimulation on the neuronal number of the cochlear nucleus (CN) was investigated by using unbiased stereological methods in rats. We found that, after 9 weeks of recovery, neurons in the anteroventral cochlear nucleus (AVCN) degenerated, whereas those in the posteroventral and dorsal cochlear nuclei (PVCN and DCN) were preserved. The noise trauma induced near complete loss of the outer hair cells throughout the cochlea, and the inner hair cells were preserved only in the more apical regions. This pattern of selective loss of AVCN neurons in this study was different from trauma induced by auditory deafferentation by mechanical compression of auditory neurons. In contrast to noise trauma, mechanical compression caused loss of neurons in the PVCN and DCN. After 5 weeks of recovery from mechanical compression, there was no loss of inner or outer hair cells. These findings indicate that auditory deprivation, induced by different experimental manipulations, can have strikingly different consequences for the central auditory system. We hypothesized that AVCN neuronal death was induced by excitotoxic mechanisms via AMPA-type glutamate receptors and that excitatory neuronal circuits developed after acoustic overstimulation protected the PVCN and DCN against neuronal death. The results of the present study demonstrate that hearing loss from different etiologies will cause different patterns of neuronal degeneration in the CN. These findings are important for enhancing the performance of cochlear implants and auditory brainstem implants, because diverse types of hearing loss can selectively affect neuronal degeneration of the CN.
Department of Otolaryngology, Head and Neck Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan. tsekiya@ent.kuhp.kyoto-u.ac.jp
Tetsuji
T
Sekiya
Agneta
A
Viberg
Ken
K
Kojima
Tatsunori
T
Sakamoto
Takayuki
T
Nakagawa
Juichi
J
Ito
Barbara
B
Canlon
D000161:Acoustic Stimulation; D000818:Animals; D002452:Cell Count; D003055:Cochlear Microphonic Potentials; D017626:Cochlear Nucleus / Q000473:pathology*; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology; D006198:Hair Cells, Auditory / Q000473:pathology; D006199:Hair Cells, Auditory, Inner / Q000473:pathology; D018072:Hair Cells, Auditory, Outer / Q000473:pathology; D006317:Hearing Loss, Noise-Induced / Q000473:pathology*; D008297:Male; D009474:Neurons / Q000473:pathology; D009622:Noise / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012683:Sensory Deprivation