[Construction of a multiple-scale implant surface with super-hydrophilicity].
OBJECTIVE
To construct a multiple-scale organized implant surface with super-hydrophilicity.


METHODS
The SiC paper polished titanium disc was sandblasted and treated with HF/HNO₃ and HCl/H₂SO₄, then acid-etched with H₂SO₄/H₂O₂. The physicochemical properties of the surfaces were characterized by scanning electron microscope, static state contact angle and X-ray diffraction. MC3T3-E1 cells were used to evaluate the effects of the surface on the cell adhesion, proliferation and differentiation.


RESULTS
The acid-etching process with a mixture of H₂SO₄/H₂O₂ superimposed the nano-scale structure on the micro-scale texture. The multiple-scale implant surface promoted its hydrophilicity and was more favorable to the responses of osteoprogenitor cells, characterized by increased DNA content, enhanced ALP activity and promoted OC production.


CONCLUSIONS
A multiple-scale implant surface with super-hydrophilicity has been constructed in this study, which facilitates cell proliferation and adhesion.
The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
Qiao-jie
QJ
Luo
Xiao-dong
XD
Li
Ying
Y
Huang
Shi-fang
SF
Zhao
D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D045662:Dental Etching; D015921:Dental Implants; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology
D015921:Dental Implants; D014025:Titanium