Prevalence of a novel division-level bacterial lineage in Lake Dhanmondi, Dhaka, Bangladesh, as revealed by deep sequencing of 16S rRNA gene amplicons.
A culture-independent study of the bacterial diversity in Lake Dhanmondi, located in the central region of Dhaka city, Bangladesh, was carried out using deep sequence analysis of 16S rRNA gene PCR amplicons. The results revealed the presence of a group of bacteria, termed LD11, phylogenetically unrelated to any previously cultivated bacteria at the phylum level. LD11 sequences comprised about 1.7 % of the total sequence reads after quality assessment. LD11 appears to constitute a novel division with a deep evolutionary lineage apparently branching between the Chloroflexi and Thermi-Deinococci phyla. Sequence similarity with molecular data from freshwater environments indicates that LD11 represents a widespread and novel clade of freshwater bacteria for which no cultivated representatives are yet available.
Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh.
Nafisa
N
Azmuda
Mohammed Ziaur
MZ
Rahman
Marit Steine
MS
Madsen
Sirajul Islam
SI
Khan
Nils-Kåre
NK
Birkeland
D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D001459:Bangladesh; D044822:Biodiversity; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S