Identification of androgen-regulated genes in human prostate.
Androgens are essential for the development of the prostate and prostate             cancer. We examined androgen-regulated gene expression in the human prostate.             Samples from benign and malignant prostate tissue and samples containing prostate             tissue obtained from prostate cancer patients three days after surgical castration             were further processed as probes for a GeneChip array. The comparison of gene             expression profiles in castrated samples and in benign or malignant prostate tissue             samples revealed androgen-regulated genes. We further evaluated the genes which             were differentially expressed in benign and malignant prostate samples. The androgen-regulated             expression of dual specificity phosphatase 1 (DUSP1) was confirmed in the LNCaP             prostate cancer cell line, as the expression of DUSP1 increased with androgen             treatment over the course of time. The expression of the genes CRISP3, PCA3, OR51E2,             HOXC6, AGR3, AMACR and SLC14A1 was affected by castration in addition to differential             expression in the benign and malignant prostate. These sample results require             further investigation for the role of AGR3 and SLC14A1 in prostate cancer as these             associations have not been reported previously.
Department of Surgery, Oulu University Hospital, and Department of Pathology, University of Oulu, FIN-90029 Oulu, Finland. markku.vaarala@oulu.fi
Markku H
MH
Vaarala
Pasi
P
Hirvikoski
Saila
S
Kauppila
Timo K
TK
Paavonen
D000728:Androgens / Q000494:pharmacology*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002369:Castration; D045744:Cell Line, Tumor; D054638:Dual Specificity Phosphatase 1 / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D000096982:Urea Transporters
D000728:Androgens; D002352:Carrier Proteins; D054638:Dual Specificity Phosphatase 1; D026901:Membrane Transport Proteins; D009363:Neoplasm Proteins; D000096982:Urea Transporters; C472588:AGR3 protein, human; C517019:DUSP1 protein, human