VEGF induces angiogenesis in a zebrafish embryo glioma model established             by transplantation of human glioma cells.
Zebrafish (Danio rerio) is becoming an increasingly popular vertebrate cancer             model. In this study, we established a xenotransplanted zebrafish embryo glioma             model to further investigate the molecular mechanisms of tumor angiogenesis. We             find that the glioma cell line U87 can survive, proliferate and induce additional             SIV branches in zebrafish embryos. In addition, by the means of in situ hybridization             and quantitive RT-PCR analyses we find that the transplanted U87 cells can induce             the ectopic zebrafish vascular endothelial growth factor A (VEGF A) and its receptor             VEGFR2/KDR mRNA expression and increase their expression levels, resulting in             additional SIV branches.
Laboratory of Integrated Biosciences, School of Life Sciences, Sun Yat-Sen University, and Department of Neurology, Sun Yat-Sen Memorial Hospital, Guangzhou 510006, PR China.
Dong
D
Li
Xiang-Pen
XP
Li
Hong-Xuan
HX
Wang
Qing-Yu
QY
Shen
Xiang-Ping
XP
Li
Lu
L
Wen
Xiu-Jiao
XJ
Qin
Qiu-Li
QL
Jia
Hsiang-Fu
HF
Kung
Ying
Y
Peng
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D004625:Embryo, Nonmammalian; D015870:Gene Expression; D005910:Glioma / Q000098:blood supply* / Q000473:pathology; D006801:Humans; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000502:physiology*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
D042461:Vascular Endothelial Growth Factor A; D029961:Zebrafish Proteins; D040301:Vascular Endothelial Growth Factor Receptor-2; D000469:Alkaline Phosphatase