In planta production of a candidate vaccine against bovine papillomavirus type 1.
Bovine papillomavirus type 1 (BPV-1) is an economically important virus that induces tumourigenic pathologies in horses and cows. Given that the BPV-1 L1 major coat protein can self-assemble into highly immunogenic higher-order structures, we transiently expressed it in Nicotiana benthamiana as a prelude to producing a candidate vaccine. It was found that plant codon optimization of L1 gave higher levels of expression than its non-optimized counterpart. Following protein extraction, we obtained high yields (183 mg/kg fresh weight leaf tissue) of relatively pure L1, which had self-assembled into virus-like particles (VLPs). We found that these VLPs elicited a highly specific and strong immune response, and therefore they may have utility as a potential vaccine. This is the first report demonstrating the viable production of a candidate BPV vaccine protein in plants.
The James Hutton Institute Dundee, Dundee DD2 5DA, UK. Andrew.Love@hutton.ac.uk
Andrew J
AJ
Love
Sean N
SN
Chapman
Slavica
S
Matic
Emanuela
E
Noris
George P
GP
Lomonossoff
Michael
M
Taliansky
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D052688:Bovine papillomavirus 1 / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D015870:Gene Expression; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011817:Rabbits; D011994:Recombinant Proteins; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D058425:Vaccines, Virus-Like Particle / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C085217:L1 protein, Bovine papillomavirus; D011994:Recombinant Proteins; D058425:Vaccines, Virus-Like Particle