[Clinical significance and detection of the expression of CD25- CD127- on CD4+ T cells in peripheral blood in patients with hepatitis B].
OBJECTIVE
To investigate the clinical significance and detection of the expression of CD25- CD127- on CD4+ T cells in peripheral blood in patients with hepatitis B.


METHODS
The expression of CD25- CD127- on CD4+ T cells were measured by using flow cytometry in 53 patients with chronic hepatitis B, 53 carrier with hepatitis B virus and 26 healthy blood donors, and follow up 20 patients with HBV-DNA positive treated with interferon.


RESULTS
(1) Compared with healthy controls, the expression of CD25- CD127- on CD4+ T cells in patients and carrier with hepatitis B virus were lower (Q = 4.559, P < 0.05; Q = 6.230, P < 0.05). (2) The expression of CD25- CD127- on CD4+ T cells in patients with HBV-DNA positive (n = 77) was lower than that of negative (n = 29) (t = 2.290, P = 0.024). (3) Compared with the prior treatment,the expression of CD25- CD127- on CD4+ T cells in patients with B hepatitis were lower after interferon treated with 12 weeks (t = 2.469, P = 0.024).


CONCLUSIONS
It suggested that the CD25- CD127- expression on CD4+ T cells correlated with viral infections and cleared,exogenous interferon could decrease CD25- CD127- expression on CD4+ T cells.
Department of Laboratory Medicine, The Taizhou People's Hospital, Jiangsu 225300, China.
Jun
J
Ye
Ya-Bao
YB
Chen
Hong-Tao
HT
Xu
Jian-Chun
JC
Xian
Li-Xing
LX
Zhang
Mei
M
Lin
Jun-Xing
JX
Huang
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D004279:DNA, Viral / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D006509:Hepatitis B / Q000276:immunology* / Q000821:virology; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000097:blood*; D053699:Interleukin-7 Receptor alpha Subunit / Q000097:blood*; D008297:Male
D004279:DNA, Viral; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D053699:Interleukin-7 Receptor alpha Subunit