Multi-contrast focal modulation microscopy for in vivo imaging of thick biological tissues.
In vivo high resolution imaging of biological tissues is desirable for a wide range of biomedical applications. Recently focal modulation microscopy (FMM) has been developed and an imaging depth comparable to multi-photon microscopy (MPM) and optical coherence microscopy (OCM) has been achieved. Here we report the first focal modulation microscope that is capable of performing real-time fluorescence and scattering imaging simultaneously on thick biological tissues. A novel spatiotemporal phase modulator (STPM) has been designed and integrated into such a microscope to achieve high performances in terms of imaging speed, contrast, effective spatial resolution, signal to noise ratio, and compatibility with multiple excitation wavelengths.
Department of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore. biecng@nus.edu.sg
Nanguang
N
Chen
Guangjun
G
Gao
D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*