Characterization of IgG responses of rabbits to Sj14-3-3 protein after experimental infection with Schistosoma japonicum.
An indirect enzyme-linked immunosorbent assay method was developed for detection of IgG against 14-3-3 protein in sera of rabbits. Rabbits infected with 500 cercariae of Schistosoma japonicum were grouped and the characterization of the IgG responses was observed. For the treated group, the IgG could be detected as early as 2-4 weeks post-infection and then their levels rose rapidly and reached a peak at around 6 weeks. After the infected rabbits were treated with praziquantel at 6 weeks post-infection, IgG levels in the sera significantly decreased. While in the untreated group, the IgG levels were constantly very low. For all infected rabbits, 60 % (six of ten) had positive reaction with 14-3-3 protein, and 40 % (four of ten) had high IgG levels. This finding would be more helpful to understand this 14-3-3 protein.
Department of Novel Biotechnology for Parasitic Diseases Prevention and Control, Jiangsu Institute of Parasitic Diseases, 117 Yangxiang, Meiyuan, Wuxi 214064, Jiangsu Province, China.
Chun-Yan
CY
Qian
Jie
J
Wang
Chuan-Xin
CX
Yu
Xu-Ren
XR
Yin
Li-Jun
LJ
Song
Wei
W
Zhang
Yi
Y
Jin
Xue-Dan
XD
Ke
D048948:14-3-3 Proteins / Q000276:immunology*; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage; D000909:Antibodies, Helminth / Q000097:blood*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D011223:Praziquantel / Q000008:administration & dosage; D011817:Rabbits; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000188:drug therapy / Q000276:immunology* / Q000469:parasitology; D013997:Time Factors
D048948:14-3-3 Proteins; D000871:Anthelmintics; D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D011223:Praziquantel