Periostin, a stroma-associated protein, correlates with tumor invasiveness and progression in nasopharyngeal carcinoma.
Recently, the tumor microenvironment is increasingly recognized as playing an important role in cancer proliferation, invasion, and metastasis. To screen stroma-associated proteins involved in nasopharyngeal carcinoma (NPC) carcinogenesis, laser capture microdissection (LCM) and quantitative proteomic analysis were employed to assess different protein expression of the stroma between NPC and normal nasopharyngeal mucosa (NNM). In this study, periostin was identified to be significantly up-regulated in NPC stroma compared with NNM stroma and the result was further confirmed by Western blotting. Immunohistochemistry showed that over-expression of periostin was frequently observed in the stroma of NPC and matched lymph node metastases (LNM) compared with the stroma of NNM. Statistical analysis showed over-expression of periostin was significantly associated with advanced clinical stage (P < 0.001) and lymph node metastasis (P < 0.001) and decreased overall survival (P < 0.001) in NPC. Cox regression analysis indicated over-expression of periostin was an independent prognostic factor. Furthermore, ectopic expression of periostin was used to examine its effect on invasiveness of NPC cell in vitro and the result showed that periostin was able to promote invasiveness of NPC cell. In conclusion, periostin expression is correlated with tumor stage, lymph node metastasis, and patient survival. Periostin is a potential biomarker for the differentiation and prognosis of NPC, and it might play an important role in the progression of NPC.
Department of Histology and Embryology, School of Medicine, University of South China, Hengyang, China.
Meixiang
M
Li
Cui
C
Li
Danjuan
D
Li
Yuanjie
Y
Xie
Jinfeng
J
Shi
Guoqing
G
Li
Yongjun
Y
Guan
Maoyu
M
Li
Pengfei
P
Zhang
Fang
F
Peng
Zhiqiang
Z
Xiao
Zhuchu
Z
Chen
D016475:3T3 Cells; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D018450:Disease Progression; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D061346:Laser Capture Microdissection; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008875:Middle Aged; D008969:Molecular Sequence Data; D009297:Nasal Mucosa / Q000378:metabolism; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D009305:Nasopharynx / Q000378:metabolism; D009361:Neoplasm Invasiveness; D040901:Proteomics; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D059016:Tumor Microenvironment
D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; C512835:POSTN protein, human