Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells.
OBJECTIVE
This study evaluated the effects of photodynamic therapy (PDT) on buccal candidiasis in mice and on the adherence of yeast to buccal epithelial cells (BECs) in vitro.


METHODS
A total of 56 immunosuppressed mice with buccal candidiasis were subjected to PDT, consisting of treatment with erythrosine (400 μmol/L) followed by exposure to a green LED (14.34 J cm(-2)). After treatment, the yeasts recovered from the mice were quantified (CFU/mL) and analyzed for the effects of PDT on their adherence to BECs. The data were analyzed using ANOVA, the Tukey test, Kruskal-Wallis test and Student t test.


RESULTS
PDT significantly reduced the amount of yeast present in the lesions by 0.73 log(10) (P = .018) and reduced C. albicans adherence to BECs by 35% without damaging adjacent tissues (P = .045).


CONCLUSIONS
Photodynamic therapy exhibited antifungal effects against C. albicans biofilms formed in vivo and reduced the capacity of C. albicans to adhere to BECs in vitro.
Department of Biosciences and Oral Diagnosis, School of Dentistry of São José dos Campos, UNESP- Univ Estadual Paulista, São José dos Campos, SP, Brazil. carol_biolog@yahoo.com.br
Anna Carolina Borges Pereira
AC
Costa
Vanessa Maria
VM
Campos Rasteiro
Emily Setsuko Halter
ES
da Silva Hashimoto
Cássia Fernandes
CF
Araújo
Cristiane Aparecida
CA
Pereira
Juliana Campos
JC
Junqueira
Antonio Olavo Cardoso
AO
Jorge
D000704:Analysis of Variance; D000818:Animals; D018441:Biofilms; D002180:Candidiasis, Oral / Q000188:drug therapy*; D002610:Cheek; D004923:Erythrosine / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D007165:Immunosuppression Therapy; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010778:Photochemotherapy / Q000379:methods*; D018709:Statistics, Nonparametric; D014059:Tongue / Q000187:drug effects / Q000382:microbiology
D005456:Fluorescent Dyes; D004923:Erythrosine