3D adaptive optics in a light sheet microscope.
We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish. We show improvements in image quality whilst recording a 3D "z-stack" and show how the aberrations come from varying depths in the fish.
Department of Physics & Biophysical Sciences Institute, Durham University, Durham DH1 3LE, UK.
Cyril
C
Bourgenot
Christopher D
CD
Saunter
Jonathan M
JM
Taylor
John M
JM
Girkin
Gordon D
GD
Love
D000818:Animals; D002138:Calibration; D049452:Green Fluorescent Proteins / Q000378:metabolism; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D008027:Light; D008029:Lighting; D008853:Microscopy / Q000295:instrumentation*; D055095:Optics and Photonics / Q000295:instrumentation*; D010969:Plastics; D012031:Refractometry; D015027:Zebrafish
D010969:Plastics; D049452:Green Fluorescent Proteins