Antibody array-based immunosensor for detecting cardiovascular disease risk markers.
Quantitative detection of proteins in multiplexed platforms presents technical advantages at clinical and laboratory settings compared to the monoplex ELISA method. With this purpose, we implemented a pilot study using in-house-designed sandwich-type antibody array for multiplexed detection of seven cardiovascular disease (CVD) risk markers and compared the performance of our immunosensor to conventional ELISA kits. Results indicated that our immunosensor can determine serum amyloid A (SAA), vascular cell adhesion molecule (VCAM), and myoglobin (MYO) concentrations accurately, precisely, and above all very much similar to ELISA. Hence, multiplexed detection and quantification of SAA, VCAM, and MYO with our immunosensor can be considered as a potential multiplexed alternative to the ELISA method.
Biological Sciences and Bioengineering Program, Sabanci University, Tuzla, Istanbul, Turkey.
Can
C
Timucin
Ozgur
O
Gul
Ozgur
O
Kutuk
Huveyda
H
Basaga
D000906:Antibodies / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques; D002318:Cardiovascular Diseases / Q000097:blood*; D019040:E-Selectin / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D009211:Myoglobin / Q000276:immunology*; D040081:Protein Array Analysis; D019947:Receptors, Interleukin-6 / Q000276:immunology; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000276:immunology; D000685:Serum Amyloid A Protein / Q000276:immunology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000276:immunology*
D000906:Antibodies; D015415:Biomarkers; D019040:E-Selectin; D009211:Myoglobin; D019947:Receptors, Interleukin-6; D047888:Receptors, Tumor Necrosis Factor, Type I; C546936:SELE protein, human; D000685:Serum Amyloid A Protein; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1