Spatio-temporal expression study of phosphorylated 70-kDa ribosomal S6 kinase (p70S6k) in mesial temporal lobe epilepsy.
OBJECTIVE
To determine the spatio-temporal expression of p70S6k activation in hippocampus in mesial temporal lobe epilepsy.


METHODS
Temporal lobe epilepsy model was established by stereotaxically unilateral and intrahippocampal injection of kainite acid (KA) in adult male C57BL/6 mice. Latent and chronic epileptogenesis were represented by mice 5 days after KA injection (n = 5) and mice 5 weeks after KA injection (n = 8), respectively. Control mice (n = 5) were injected with saline. Immunohistochemical assays were performed on brain sections of the mice.


RESULTS
Hippocampus both ipsilateral and contralateral to the KA injection displayed significantly up-regulated pS6 immunoreactivity in dispersed granule cells in 5-day and 5-week model mice.


CONCLUSIONS
The activation of p70S6k is mainly located in the dentate gyrus in KA-induced mouse model of temporal lobe epilepsy, indicating that the activation may be related with the disperse degree and hypertrophy of granule cells.
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China.
Xiao-Liang
XL
Xing
Long-Ze
LZ
Sha
Yuan
Y
Yao
Yan
Y
Shen
Li-Wen
LW
Wu
Qi
Q
Xu
D000818:Animals; D004833:Epilepsy, Temporal Lobe / Q000201:enzymology*; D006624:Hippocampus / Q000201:enzymology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000032:analysis* / Q000378:metabolism
D038762:Ribosomal Protein S6 Kinases, 70-kDa