Antifungal activity of coronarin D against Candida albicans.
OBJECTIVE
The objective of this study was to investigate the antifungal activity of coronarin D on Candida albicans and its activity was compared with clotrimazole and nystatin.


METHODS
Coronarin D was extracted by liquid chromatography and used in antifungal testing. The inhibitory effect of coronarin D on C. albicans was determined by cultures and an applied broth dilution test. The rate of fungicidal activity was evaluated by time-kill curves. Morphologic alterations of fungal cells were investigated using scanning electron microscopy.


RESULTS
Coronarin D was effective against C. albicans; the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were 2 and 4 mg/mL, respectively. The C. albicans killing activity of coronarin D was higher than clotrimazole and nystatin at 2 × MFC and 4 × MFC, respectively. Morphologic alterations of fungal cells consistent with cell membrane damage were observed in the coronarin D-treated cells.


CONCLUSIONS
Coronarin D showed promising antifungal activity against C. albicans in vitro.
Instructor, Department of Oral Diagnosis, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand. Ruchadapornk@yahoo.com
Ruchadaporn
R
Kaomongkolgit
Kusuma
K
Jamdee
Sorapong
S
Wongnoi
Nitirat
N
Chimnoi
Supanna
S
Techasakul
D000704:Analysis of Variance; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D003022:Clotrimazole / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D009761:Nystatin / Q000494:pharmacology
D000935:Antifungal Agents; D004224:Diterpenes; C532843:coronarin D; D009761:Nystatin; D003022:Clotrimazole