Gene expression profiles of HLA-G1 overexpressed in hES cells.
The goals of this study were to analyze the change in the global gene expression profile of exogenous human leukocyte antigen-G1 (HLA-G1) overexpressed in human embryonic stem (hES) cells and to explore the molecular mechanism by which the overexpression of HLA-G1 modifies immunologic pathways. Microarray and quantitative real-time PCR analyses were performed to quantify the differential expression pattern of HLA-G1 + H1 hES cells. The results showed that HLA-G1 differentially regulated the expression of 425 genes with at least a twofold increase or decrease. These differentially expressed genes were classified into 13 functional groups, including cellular components, biological processes, and molecular functions. The pathways of focal adhesion, the TGF-β signaling pathway, and the immune response were the most predominantly affected. The synergism of these genes could explain the mechanism of the immunosuppression of HLA-G1 + H1 hES cells. Thus, the expression pattern reflected a broad spectrum of roles of HLA-G1 in hES cells.
Department of Obstetrics and Gynecology, General Hospital of the People's Liberation Army, Fuxing Road 28, Beijing 100853, China.
Yajing
Y
Zhu
Sanjun
S
Zhao
Hongxi
H
Zhao
Yuanqing
Y
Yao
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D053595:Embryonic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism* / Q000637:transplantation; D022001:Focal Adhesions; D005786:Gene Expression Regulation; D059951:HLA-G Antigens / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D013724:Teratoma / Q000378:metabolism; D014158:Transcription, Genetic; D059467:Transcriptome; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000378:metabolism
D059951:HLA-G Antigens; D016212:Transforming Growth Factor beta