Characterization of a porcine intestinal epithelial cell line for influenza virus production.
We have developed a porcine intestine epithelial cell line, designated SD-PJEC for the propagation of influenza viruses. The SD-PJEC cell line is a subclone of the IPEC-J2 cell line, which was originally derived from newborn piglet jejunum. Our results demonstrate that SD-PJEC is a cell line of epithelial origin that preferentially expresses receptors of oligosaccharides with Sia2-6Gal modification. This cell line is permissive to infection with human and swine influenza A viruses and some avian influenza viruses, but poorly support the growth of human-origin influenza B viruses. Propagation of swine-origin influenza viruses in these cells results in a rapid growth rate within the first 24 h post-infection and the titres ranged from 4 to 8 log(10) TCID(50) ml(-1). The SD-PJEC cell line was further tested as a potential alternative cell line to Madin-Darby canine kidney (MDCK) cells in conjunction with 293T cells for rescue of swine-origin influenza viruses using the reverse genetics system. The recombinant viruses A/swine/North Carolina/18161/02 (H1N1) and A/swine/Texas/4199-2/98 (H3N2) were rescued with virus titres of 7 and 8.25 log(10) TCID(50) ml(-1), respectively. The availability of this swine-specific cell line represents a more relevant substrate for studies and growth of swine-origin influenza viruses.
Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
Zhi
Z
Sun
Division of Basic Biomedical Sciences, Sanford School of Medicine, The University of South Dakota, Vermillion, SD 57069, USA.
Victor C
VC
Huber
Division of Basic Biomedical Sciences, Sanford School of Medicine, The University of South Dakota, Vermillion, SD 57069, USA.
Kara
K
McCormick
Department of Biology/Microbiology, South Dakota State University, Brookings, SD 57007, USA.|Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
Radhey S
RS
Kaushik
Division of Infectious Diseases, Department of Internal Medicine, Department of Molecular Microbiology, Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA.
Adrianus C M
ACM
Boon
Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
Longchao
L
Zhu
Newport Laboratories, Worthington, MN 56187, USA.|Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
Ben
B
Hause
Department of Infectious Diseases, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
Richard J
RJ
Webby
Department of Biology/Microbiology, South Dakota State University, Brookings, SD 57007, USA.|Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
Ying
Y
Fang
D000818:Animals; D000831:Animals, Newborn; D001717:Birds; D002460:Cell Line / Q000821:virology*; D004285:Dogs; D004847:Epithelial Cells / Q000821:virology*; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development* / Q000502:physiology; D009981:Influenza B virus / Q000254:growth & development* / Q000502:physiology; D005585:Influenza in Birds / Q000821:virology; D007251:Influenza, Human / Q000821:virology; D007583:Jejunum / Q000166:cytology / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000821:virology; D014776:Virus Cultivation / Q000295:instrumentation* / Q000379:methods; D014779:Virus Replication