[Effect of zedoary oil for cat D and cat K expression in A549 cell line].
OBJECTIVE
To explore the Zedoary oil on A549 cell line of collagen deposition cat D and cat K expression.


METHODS
The A549 cell line were treat by Zedoary oil on four different concentrations (0, 40, 80, 120 mg x L(-1)) in different time. Dynamic changes of collagen in A549 cell using Picric-sirius red method. Cat D and Cat K expression of level were detected by using western blot.


RESULTS
The collagen content showed that Zedoary oil had an inhibitory effect on the deposition of A549 cells. The results of western blot showed that the expression of cat D and cat K were up-regulated significangly in A549 cells of Zedoary oil groups compared with that in controls.


CONCLUSIONS
A549 cell of collagen deposition were reduced by Zedoary oil. The effects may due to the up-regulation of cat D and cat K.
Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China.
Changfu
C
Yang
Chunfang
C
Huang
Xiaofang
X
Sun
Jianzhao
J
Niu
Jifeng
J
Wang
D000818:Animals; D015153:Blotting, Western; D002402:Cathepsin D / Q000378:metabolism*; D056657:Cathepsin K / Q000378:metabolism*; D045744:Cell Line, Tumor; D003094:Collagen / Q000378:metabolism; D030024:Curcuma / Q000737:chemistry*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology*; D015854:Up-Regulation
D010938:Plant Oils; D003094:Collagen; D056657:Cathepsin K; D002402:Cathepsin D; C533923:Ctsd protein, mouse