Nucleus-encoded mRNAs for chloroplast proteins GapA, PetA, and PsbO are trans-spliced in the flagellate Euglena gracilis irrespective of light and plastid function.
Euglena gracilis is a fresh-water flagellate possessing secondary chloroplasts of green algal origin. In contrast with organisms possessing primary plastids, mRNA levels of nucleus-encoded genes for chloroplast proteins in E. gracilis depend on neither light nor plastid function. However, it remains unknown, if all these mRNAs are trans-spliced and possess spliced leader sequence at the 5'-end and if trans-splicing depends on light or functional plastids. This study revealed that polyadenylated mRNAs encoding the chloroplast proteins glyceraldehyde-3-phosphate dehydrogenase (GapA), cytochrome f (PetA), and subunit O of photosystem II (PsbO) are trans-spliced irrespective of light or plastid function.
Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynská dolina, 842 15 Bratislava, Slovakia.
Bianka
B
Mateášiková-Kováčová
Matej
M
Vesteg
Hana
H
Drahovská
Kristína
K
Záhonová
Rostislav
R
Vacula
Juraj
J
Krajčovič
D060365:Chloroplast Proteins / Q000235:genetics*; D045348:Cytochromes f / Q000235:genetics; D005056:Euglena gracilis / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D005786:Gene Expression Regulation; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics; D008027:Light; D045332:Photosystem II Protein Complex / Q000235:genetics; D018087:Plastids / Q000378:metabolism / Q000528:radiation effects; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism*
D060365:Chloroplast Proteins; D045332:Photosystem II Protein Complex; D012333:RNA, Messenger; D045348:Cytochromes f; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)