Production of torularhodin, torulene, and β-carotene by Rhodotorula yeasts.
Yeasts of the genera Rhodotorula are able to synthesize different pigments of high economic value like β-carotene, torulene, and torularhodin, and therefore represent a biotechnologically interesting group of yeasts. However, the low production rate of pigment in these microorganisms limits its industrial application. Here we describe some strategies to obtain hyperpigmented mutants of Rhodotorula mucilaginosa by means of ultraviolet-B radiation, the procedures for total carotenoids extraction and quantification, and a method for identification of each pigment.
Laboratorio de Microbiología Aplicada y Biotecnología Bariloche, INIBIOMA (CONICET-Universidad del COMAHUE), San Carlos de Bariloche, Río Negro, Argentina. mmoline@comahue-conicet.gob.ar
Martín
M
Moliné
Diego
D
Libkind
María
M
van Broock
D002338:Carotenoids / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D060847:Metabolic Engineering / Q000379:methods*; D016296:Mutagenesis; D009154:Mutation; D012248:Rhodotorula / Q000235:genetics* / Q000378:metabolism*; D019207:beta Carotene / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification
D019207:beta Carotene; D002338:Carotenoids; C078922:torulene; C078923:torularhodin