Carotenoids production in different culture conditions by Sporidiobolus pararoseus.
Carotenoids produced by Sporidiobolus pararoseus were studied. It was found that biomass was connected with carbon source, temperature, and pH, but carotenoids proportion was seriously influenced by dissolved oxygen and nitrogen source. Different carotenoids could be obtained by using selected optimum conditions. In the end we established the strategies to produce β-carotene or torulene. Fed-batch fermentation in fermentor was used to prove the authenticity of our conclusions. The cell biomass, β-carotene content, and β-carotene proportion could reach 56.32 g/L, 18.92 mg/L and 60.43%, respectively, by using corn steep liquor at 0-5% of dissolved oxygen saturation. β-Carotene content was 271% higher than before this addition. The cell biomass, torulene content, and torulene proportion could reach 62.47 g/L, 31.74 mg/L, and 70.41%, respectively, by using yeast extract at 30-35% of dissolved oxygen saturation. Torulene content was 152% higher than before this addition. The strategy for enhancing specific carotenoid production by selected fermentation conditions may provide an alternative approach to enhance carotenoid production with other strains.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, Wuxi, JiangSu Province, P. R. China.
Mei
M
Han
Qian
Q
He
Wei-Guo
WG
Zhang
D001487:Basidiomycota / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D002338:Carotenoids / Q000378:metabolism; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D013696:Temperature; D019207:beta Carotene / Q000032:analysis / Q000378:metabolism*
D019207:beta Carotene; D002338:Carotenoids; C078922:torulene; D009584:Nitrogen; D010100:Oxygen