Diversity of rhizobia associated with leguminous trees growing in South Korea.
This study was carried out to examine the diversity of 34 isolates collected from 11 species of leguminous trees growing in South Korea. Phylogenetic relationships between these 34 isolates and reference strains of the Azorhizobium, Bradyrhizobium, Mesorhizobium, Rhizobium and Ensifer/Sinorhizobium were analysed by using 16S rRNA gene sequences. Twenty-one isolates were related to Mesorhizobium, four isolates to Rhizobium, and nine isolates to Bradyrhizobium. But none of isolates were related to Sinorhizobium/Ensifer and Azorhizobium. Robinia pseudoacacia and Amorpha fruticosa were nodulated by various genotypes of rhizobia out of them, most of the isolates belonged to the genus Mesorhizobium. The isolates from Lespedeza bicolar belonged to diverse genera of Mesorhizobium, Rhizobium, and Bradyrhizobium. The isolates from Maackia amurensis and Lespedeza maximowiezii var. tomentella were phylogenetically related to the genera of Bradyrhizobium. PCR-based RAPD method and phylogenetic analysis of the 16S rRNA results revealed a high phylogenetic diversity of rhizobial strains nodulating leguminous trees in South Korea. Also, the relationships between host and bacterial phylogenies showed that only Robinia pseudoacacia, and Wisteria floribunda have significantly unique branch length than expected by chance based on phylogenetic tree.
School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, WA, USA.
Jun Won
JW
Kang
Jaekyeong
J
Song
Sharon L
SL
Doty
Don Koo
DK
Lee
D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D020369:Bradyrhizobium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics; D007887:Fabaceae / Q000254:growth & development / Q000382:microbiology*; D020459:Genes, rRNA; D029884:Lespedeza / Q000382:microbiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D056910:Republic of Korea; D012231:Rhizobium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D031301:Robinia / Q000382:microbiology; D017422:Sequence Analysis, DNA; D032447:Wisteria / Q000382:microbiology
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S