[Development and application of perfusion culture producing seed cells in WAVE bioreactor].
In recent years, Chinese hamster ovary (CHO) production vessel volume has reached more than 1 000 L in Chinese biopharms, and 10 000 L in foreign big biopharms, such as Lonza and Genetech. In general, there are some steps seed bioreactor for seed expansion, which decreases the efficiency of production process. In this work, a perfusion-based process was developed to drastically increase the split ratio during the scale-up of CHO cell cultures. Fed-batch cultures were inoculated with cells propagated in either batch or perfusion cultures that grown in disposable Cellbags using the WAVE Bioreactor system. The higher cell concentration of 2 x 10(7) cells/mL with 95% viability allowed to increase the split ratio to about 1:50-1:100 for inoculum propagated in perfusion culture. The method described here could reduce the number of required expansion steps and eliminate two or three bioreactors. Disposable perfusion bioreactor with only a few liters working volume have the potential to directly inoculate volumes of up to 1 000 liters. This would allow to shorten process time in these bioreactors, which often are the bottleneck in plant throughput.
Fast Trak Center, GE (China) Research and Development Center Co. Ltd., Shanghai 201203, China.
Jianjun
J
Yang
Lili
L
Sui
D000818:Animals; D019149:Bioreactors; D016466:CHO Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus