Structural insights of rohu TLR3, its binding site analysis with fish reovirus dsRNA, poly I:C and zebrafish TRIF.
In response to double stranded RNA (dsRNA) viruses, toll-like receptor 3 (TLR3) in fish activates signaling like human, and induces innate immunity. This suggested the existence of dsRNA binding domains in fish TLR3 as reported in higher vertebrates. In in silico analysis, leucine rich repeat (LRR) regions (4-6, 13-14, 20-22), and LRR (8-15, 17-24) were identified as key domains in rohu TLR3 as poly I:C and dsRNA of fish reovirus (AGCRV,VHSV and IHNV) binding regions. 3D-models of rohu TLR3-TIR and zebrafish TRIF were generated by homology and ab initio modeling respectively, and their interacting domains were predicted. This is the first report of TLR3 modeling in fish.
Fish Health Management Division, Central Institute of Freshwater Aquaculture-CIFA, Kausalyaganga, Bhubaneswar 751002, Orissa, India.
Bikash R
BR
Sahoo
Madhubanti
M
Basu
Banikalyan
B
Swain
Jitendra
J
Maharana
Manas R
MR
Dikhit
Pallipuram
P
Jayasankar
Mrinal
M
Samanta
D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003530:Cyprinidae / Q000821:virology*; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011070:Poly I-C / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D012087:Reoviridae; D051196:Toll-Like Receptor 3 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism*
D033942:Adaptor Proteins, Vesicular Transport; D029941:Fish Proteins; D006023:Glycoproteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; C520367:TICAM1 protein, zebrafish; D051196:Toll-Like Receptor 3; D029961:Zebrafish Proteins; D011070:Poly I-C