Microsatellite marker development for the rubber tree (Hevea brasiliensis): characterization and cross-amplification in wild Hevea species.
BACKGROUND
The rubber tree (Hevea brasiliensis) is native to the Amazon region and it is the major source of natural rubber in the world. Rubber tree breeding is time-consuming and expensive. However, molecular markers such as microsatellites can reduce the time required for these programs. This study reports new genomic microsatellite markers developed and characterized in H. brasiliensis and the evaluation of their transferability to other Hevea species.


RESULTS
We constructed di- and trinucleotide-enriched libraries. From these two libraries, 153 primer pairs were designed and initially evaluated using 9 genotypes of H. brasiliensis. A total of 119 primer pairs had a good amplification product, 90 of which were polymorphic. We chose 46 of the polymorphic markers and characterized them in 36 genotypes of H. brasiliensis. The expected and observed heterozygosities ranged from 0.1387 to 0.8629 and 0.0909 to 0.9167, respectively. The polymorphism information content (PIC) values ranged from 0.097 to 0.8339, and the mean number of alleles was 6.4 (2-17). These 46 microsatellites were also tested in 6 other Hevea species. The percentage of transferability ranged from 82% to 87%. Locus duplication was found in H. brasiliensis and also in 5 of other species in which transferability was tested.


CONCLUSIONS
This study reports new microsatellite markers for H. brasiliensis that can be used for genetic linkage mapping, quantitative trait loci identification and marker- assisted selection. The high percentage of transferability may be useful in the evaluations of genetic variability and to monitor introgression of genetic variability from different Hevea species into breeding programs.
Centro de Biologia Molecular e Engenharia Genética (CBMEG) - Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, CP 6010, CEP 13083-970, Campinas, SP, Brazil. camila.mantello@gmail.com
Camila C
CC
Mantello
Fernando I
FI
Suzuki
Livia M
LM
Souza
Paulo S
PS
Gonçalves
Anete P
AP
Souza
D001947:Breeding; D018744:DNA, Plant / Q000235:genetics*; D018900:Dinucleotide Repeats; D020440:Gene Duplication; D018506:Gene Expression Regulation, Plant; D005787:Gene Frequency; D015723:Gene Library; D056426:Genetic Loci; D018745:Genome, Plant; D005838:Genotype; D006579:Heterozygote; D028482:Hevea / Q000235:genetics*; D018895:Microsatellite Repeats; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013045:Species Specificity; D018911:Trinucleotide Repeats
D018744:DNA, Plant