[Study on new toxicity-reducing methods of pinellia rhizoma prepared by ethanol (I)-new methods and technology].
OBJECTIVE
To explore new toxicity-reducing methods of Pinellia Rhizoma prepared by ethanol and the latest technical parameters.


METHODS
Pinellia Rhizoma is prepared with ethanol. The orthogonal experimental design was adopted for investigating amount of ethanol, preparing time, ethanol concentration and preparing temperature. The optimal technology was determined by the comprehensive score of toxicological indicators of PGE2 content of rat celiac percolate, with the rabbit conjunctival irritation test as the intuitive validation on toxicology reduction. The pharmacodynamics validation was used to determine the reasonability of the preparation process.


RESULTS
The optimal technology was that Pinellia Rhizoma was prepared by 75% ethanol at the temperature of 60 degrees C by 4 days, and then dried. The effect of relieving cough, reducing sputum and anti-inflammatory of Pinellia Rhizoma is not reduced after prepared by ethanol.


CONCLUSIONS
The optimal technology of Pinellia Rhizoma prepared by ethanol is simple and reasonable that it can be used as the new method to reduce toxicity and keep efficacy of Pinellia Rhizoma.
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210029, China. taowenting@126.com
Wenting
W
Tao
Hongli
H
Yu
Hao
H
Wu
Fageng
F
Zhu
Yunying
Y
Qiu
Tengfei
T
Zhao
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D003371:Cough / Q000188:drug therapy; D003890:Desiccation; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry*; D005260:Female; D006358:Hot Temperature; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D030021:Pinellia / Q000737:chemistry* / Q000633:toxicity; D010946:Plants, Medicinal / Q000737:chemistry / Q000633:toxicity; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry / Q000633:toxicity; D013183:Sputum / Q000187:drug effects; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D018675:Toxicity Tests
D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D000431:Ethanol