New insights in cellular immune response in colostrum-deprived pigs after immunization with subunit and commercial vaccines against Glässer's disease.
Four groups of colostrum-deprived pigs were immunized with Porcilis Glässer® (PG) or with subunit vaccines developed by us (rTbpA, NPAPT(M) or NPAPT(Cp)) against Glässer's disease, and they were challenged with 3×10(8)CFU of Haemophilus parasuis. A strong reduction in CD3(+)γδTCR(+) cells was seen in non-immunized control and scarcely protected (rTbpA) groups, suggesting that these cells could represent a target of H. parasuis infection. A significant increase in CD172α(+)CD163(+) cells was detected in all groups but PG, while a reduction in SLAIIDR(+) molecules expression was observed after challenge in control animals. Significant increases in CD3ε(+)CD8α(+)CD8β(+) and B cells were detected respectively in control and NPAPT groups, and in scarcely (rTbpA) and well-protected (NPAPT(M) and NPAPT(Cp)) groups. Finally, a greater response in CD4(+)CD8α(-) cells was observed in NPAPT(Cp) compared to NPAPT(M) and PG groups. These results state the potential of NPAPT antigen for developing effective vaccines against Glässer's disease.
Microbiology and Immunology Section, Department of Animal Health, University of León, 24007 León, Spain.
R
R
Frandoloso
S
S
Martínez-Martínez
S
S
Yubero
E F
EF
Rodríguez-Ferri
C B
CB
Gutiérrez-Martín
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D003126:Colostrum / Q000276:immunology*; D005260:Female; D006192:Haemophilus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D018073:Haemophilus Vaccines / Q000276:immunology / Q000627:therapeutic use*; D044137:Haemophilus parasuis / Q000276:immunology*; D007111:Immunity, Cellular; D007963:Leukocytes, Mononuclear / Q000276:immunology; D011247:Pregnancy; D013552:Swine / Q000276:immunology*; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology / Q000627:therapeutic use
D015703:Antigens, CD; D018073:Haemophilus Vaccines; D022223:Vaccines, Subunit