Serum adiponectin level is correlated with the size of HDL and LDL particles determined by high performance liquid chromatography.
OBJECTIVE
Adiponectin (APN) improves insulin resistance and prevents atherosclerosis, and HDL removes cholesterol from atherosclerotic lesions. We have demonstrated that serum HDL-cholesterol (HDL-C) and APN concentrations are positively correlated and that APN accelerates reverse cholesterol transport (RCT) by increasing HDL synthesis in the liver and cholesterol efflux from macrophages. We previously reported that APN reduced apolipoprotein (apo) B secretion from the liver. It is well-known that insulin resistance influences the lipoprotein profile. In this study, we investigated the clinical significance of APN levels and insulin resistance in lipoprotein metabolism.


METHODS
We investigated the correlation between serum APN concentration, HOMA-R, the lipid concentrations and lipoprotein particle size by high-performance liquid chromatography (HPLC) in 245 Japanese men during an annual health checkup.


RESULTS
Serum APN level was positively correlated with the cholesterol content in large LDL and HDL particles, but inversely correlated with the cholesterol content in large VLDL and small LDL particles. HOMA-R was negatively correlated with the cholesterol content in large LDL and HDL particles and positively correlated with the cholesterol content in large VLDL and small LDL particles. By multivariate analysis, APN was correlated with the particle size of LDL-C and HDL-C independently of age, BMI and HOMA-R.


CONCLUSIONS
APN may be associated with the formation of both HDL and LDL particles, reflecting the enhancement of RCT and the improvement in TG-rich lipoprotein metabolism and insulin resistance.
Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
Kazumi
K
Tsubakio-Yamamoto
Taizo
T
Sugimoto
Makoto
M
Nishida
Rieko
R
Okano
Yasumasa
Y
Monden
Rika
R
Kitazume-Taneike
Taiji
T
Yamashita
Hajime
H
Nakaoka
Ryota
R
Kawase
Miyako
M
Yuasa-Kawase
Miwako
M
Inagaki
Kazuhiro
K
Nakatani
Daisaku
D
Masuda
Tohru
T
Ohama
Akifumi
A
Matsuyama
Yumiko
Y
Nakagawa-Toyama
Masato
M
Ishigami
Issei
I
Komuro
Shizuya
S
Yamashita
D052242:Adiponectin / Q000097:blood*; D000328:Adult; D000368:Aged; D001053:Apolipoproteins / Q000097:blood; D044466:Asian People / Q000706:statistics & numerical data*; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D059168:Carotid Intima-Media Thickness; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood*; D015243:Cholesterol, VLDL / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D003430:Cross-Sectional Studies; D006801:Humans; D007333:Insulin Resistance; D007564:Japan; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D010316:Particle Size
D052242:Adiponectin; D001053:Apolipoproteins; D015415:Biomarkers; D001786:Blood Glucose; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL