Separase-dependent cleavage of pericentrin B is necessary and sufficient for centriole disengagement during mitosis.
Centriole disengagement is considered an essential step for licensing a new round of centriole duplication in the next cell cycle. Separase is critical for centriole disengagement. Here, we showed that pericentrin B (PCNTB) is specifically cleaved by separase at the exit of mitosis. The cleavage-resistant PCNTB mutant blocks the centriole disengagement and duplication. We also observed that an artificial cleavage of PCNTB during M phase induced premature disengagement of centrioles. Based on these results, we concluded that the separase-dependent cleavage of PCNTB is necessary and sufficient for centriole disengagement during mitosis.
Department of Biological Sciences, Seoul National University, Korea.
Kwanwoo
K
Lee
Kunsoo
K
Rhee
D000595:Amino Acid Sequence; D000941:Antigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D002502:Centrioles / Q000378:metabolism*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D009154:Mutation; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D064247:Separase
D000941:Antigens; D018797:Cell Cycle Proteins; D034741:RNA, Small Interfering; C086178:pericentrin; D010450:Endopeptidases; C099488:TEV protease; C577987:ESPL1 protein, human; D064247:Separase