Biosynthesis of coumarin glycosides by transgenic hairy roots of Polygonum multiflorum.
To investigate the substrate specificity and regio-selectivity of coumarin glycosyltransferases in transgenic hairy roots of Polygonum multiflorum, esculetin (1) and eight hydroxycoumarins (2-9) were employed as substrates. Nine corresponding glycosides (10-18) involving four new compounds, 6-chloro-4-methylcoumarin 7-O-β-D-glucopyranoside (15), 6-chloro-4-phenylcoumarin 7-O-β-D-glucopyranoside (16), 8-hydroxy-4-methylcoumarin 7-O-β-D-glucopyranoside (17), and 8-allyl-4-methylcoumarin 7-O-β-D-glucopyranoside (18), were biosynthesized by the hairy roots.
Department of Natural Products Chemistry, Jinan University, Guangzhou, China.
Liangbin
L
Zhou
Tian
T
Tian
Bailin
B
Xue
Liyan
L
Song
Ling
L
Liu
Rongmin
R
Yu
D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000737:chemistry / Q000378:metabolism*; D006027:Glycosides / Q000096:biosynthesis* / Q000737:chemistry; D016695:Glycosyltransferases / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000201:enzymology; D028502:Polygonum / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D013237:Stereoisomerism; D013379:Substrate Specificity
D003374:Coumarins; D006027:Glycosides; D016695:Glycosyltransferases