Immortalization of human B cells: analysis of B cell repertoire and production of human monoclonal antibodies.
One of the major challenges in human B cell immunology field has been the objective to establish stable monoclonal cells lines that express the B cell receptor (BCR) on their cell surface and secrete antibodies. Such a system is extremely attractive not only for studying various aspect of BCR signaling but also for the generation of human monoclonal antibody and the analysis of the human B cell repertoire. This chapter describes an efficient method to immortalize and clone human B cells by Epstein-Barr Virus (EBV) transformation.
Translational Science, Novartis Institute for Biomedical Research, Basel, Switzerland. elisabetta.traggiai@novartis.com
Elisabetta
E
Traggiai
D000911:Antibodies, Monoclonal / Q000235:genetics / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans
D000911:Antibodies, Monoclonal