Determination of host RNA helicases activity in viral replication.
RNA helicases are encoded by all eukaryotic and prokaryotic cells and a minority of viruses. Activity of RNA helicases is necessary for all steps in the expression of cells and viruses and the host innate response to virus infection. Their vast functional repertoire is attributable to the core ATP-dependent helicase domain in conjunction with flanking domains that are interchangeable and engage viral and cellular cofactors. Here, we address the important issue of host RNA helicases that are necessary for replication of a virus. This chapter covers approaches to identification and characterization of candidate helicases and methods to define the biochemical and biophysical parameters of specificity and functional activity of the enzymes. We discuss the context of cellular RNA helicase activity and virion-associated RNA helicases. The methodology and choice of controls fosters the assessment of the virologic scope of RNA helicases across divergent cell lineages and viral replication cycles.
Department of Veterinary Biosciences, Ohio State University, Columbus, Ohio, USA.
Amit
A
Sharma
Kathleen
K
Boris-Lawrie
D000818:Animals; D023281:Genomics / Q000379:methods; D006801:Humans; D040901:Proteomics / Q000379:methods; D020365:RNA Helicases / Q000378:metabolism*; D014779:Virus Replication / Q000235:genetics / Q000502:physiology
D020365:RNA Helicases