The effect of magnesium and vitamin E pre-treatments on irradiation-induced oxidative injury of cardiac and pulmonary tissues in rats: a randomized experimental study.
OBJECTIVE
The aim of this study was to investigate the effect of pre-treatment with the free radical scavenging molecules, magnesium and vitamin E, on lipid peroxidation to limit radiation-induced heart and lung injury.


METHODS
Female Sprague-Dawley rats were divided into 4 groups by a simple randomization method as saline-treated control (n=4), saline-treated irradiated (IR; n=6), magnesium sulphate-treated irradiation (IR) (Mg+IR; n=6) and vitamin E-treated IR (vit E+IR; n=6), respectively. The animals were given either saline, Mg (600 mg/kg/day) or vit E (100 mg/kg/day) intraperitoneally for five days prior to irradiation. Twelve hours after the fifth injection, animals in irradiation groups were irradiated to 20 Gy using 6 MV photons in linear accelerator. Twenty-four hours later cardiac and lung tissue samples were obtained for determination of myeloperoxidase activity (MPO), malondialdehyde (MDA) levels, and luminol and lucigenin levels measured by chemiluminescence (CL) methods.


RESULTS
No significant changes were observed between cardiac and pulmonary MDA and CL results of the experimental groups. However, cardiac and pulmonary MPO activities in the saline-treated IR group were increased as compared to control group (p<0.05 for all), while in the Mg-pretreated and vit E pretreated groups neutrophil infiltration was reduced, reaching to statistical significance only in the Mg-pretreated group (p<0.05).


CONCLUSIONS
Prophylactic use of magnesium sulfate has limited the infiltration of neutrophils to both the cardiac and pulmonary tissues at the early 24 h of irradiation. However, how limiting neutrophils as the sources of free radicals and inflammatory mediators would alter oxidative stress of heart and lung tissues in the long-term is not clear yet.
Department of Radiation Oncology, Vocational School of Health Related Professions, Marmara University, İstanbul-Turkey. bmatasoy@yahoo.com
Beste M
BM
Atasoy
Zerrin
Z
Özgen
Korkut
K
Bostancı
Meral
M
Yüksel
Zeynep
Z
Özen
Roman
R
İbrahimov
Ufuk
U
Abacıoğlu
D000166:Acridines / Q000032:analysis; D000818:Animals; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology / Q000627:therapeutic use; D006321:Heart / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects*; D008163:Luminescent Measurements; D008165:Luminol / Q000032:analysis; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D008274:Magnesium / Q000494:pharmacology* / Q000627:therapeutic use; D008315:Malondialdehyde / Q000032:analysis; D009195:Peroxidase / Q000032:analysis; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000494:pharmacology* / Q000627:therapeutic use
D000166:Acridines; D016166:Free Radical Scavengers; D014810:Vitamin E; C033472:10,10'-dimethyl-9,9'-biacridinium; D008315:Malondialdehyde; D008165:Luminol; D009195:Peroxidase; D008274:Magnesium