Development of reversible fluorescence probes based on redox oxoammonium cation for hypobromous acid detection in living cells.
We describe the synthesis, properties, and application of two reversible fluorescent probes, mCy-TemOH and Cy-TemOH, for HOBr sensing and imaging in live cells. The two probes contain a hydroxylamine functional group for the monitoring of HOBr oxidation/ascorbic acid reduction events. Confocal fluorescence microscopy has established the HOBr detection in live-cells.
State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, CAS, P. R. China.
Fabiao
F
Yu
Ping
P
Song
Peng
P
Li
Bingshuai
B
Wang
Keli
K
Han
D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry; D001959:Bromates / Q000032:analysis*; D001965:Bromides / Q000494:pharmacology; D002460:Cell Line; D003497:Cyclic N-Oxides / Q000138:chemical synthesis*; D047209:Eosinophil Peroxidase / Q000378:metabolism / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006898:Hydroxylamines / Q000737:chemistry; D007211:Indoles / Q000138:chemical synthesis*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D057054:Molecular Imaging / Q000379:methods*; D010084:Oxidation-Reduction; D017680:Potassium Compounds / Q000494:pharmacology; D013050:Spectrometry, Fluorescence
D001959:Bromates; D001965:Bromides; D003497:Cyclic N-Oxides; D005456:Fluorescent Dyes; D006898:Hydroxylamines; D007211:Indoles; D017680:Potassium Compounds; D006861:Hydrogen Peroxide; D047209:Eosinophil Peroxidase; C027664:hypobromous acid; C039004:potassium bromide; D001205:Ascorbic Acid