Curing viruses in Pleurotus ostreatus by growth on a limited nutrient medium containing cAMP and rifamycin.
Oyster mushroom spherical virus (OMSV) and oyster mushroom isometric virus (OMIV) are the causative agents of a fruiting body deformation disease in the edible mushroom Pleurotus ostreatus. The curing of these mycoviruses was facilitated by a serial transfer of infected mycelia onto a limited nutrient medium containing 1mM of cAMP and 75 μg/ml of rifamycin (cAMP-rifamycin plate). The mycelia were grown on cAMP-rifamycin plates for 5 successive passages. ELISA and RT-PCR showed that the amount of mycoviruses inside the mycelia decreased significantly with increasing numbers of passages. The mycelia became free of viruses after 5 successive passages. Cultivation of the virus-cured mycelia on a mushroom compost medium produced a normal harvest, whereas the spawn infected with viruses failed to produce any fruiting bodies.
Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University, 900 Gajwa-Dong, Jinju, Republic of Korea.
Y C
YC
Kwon
D W
DW
Jeong
S I
SI
Gim
H S
HS
Ro
H S
HS
Lee
D000998:Antiviral Agents / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D000242:Cyclic AMP / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D009172:Mycology / Q000379:methods*; D020076:Pleurotus / Q000254:growth & development / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012294:Rifamycins / Q000378:metabolism*; D014780:Viruses / Q000302:isolation & purification*
D000998:Antiviral Agents; D003470:Culture Media; D012294:Rifamycins; D000242:Cyclic AMP