An ultrathin poly(L-lactic acid) nanosheet as a burn wound dressing for protection against bacterial infection.
Burn wounds are highly susceptible to bacterial infection due to impairment of the skin's integrity. Therefore, prevention of bacterial colonization/infection in the wound is crucial for the management of burns, including partial-thickness burn injuries. Although partial-thickness burn injuries still retain the potential for reepithelialization, the complication of wound infection severely impairs the reepithelialization even in such superficial burn injuries. We recently developed a biocompatible nanosheet consisting of poly(L-lactic acid) (PLLA). The PLLA nanosheets have many useful and advantageous biological properties for their application as a wound dressing, such as sufficient flexibility, transparency, and adhesiveness. We herein investigated the suitability of the PLLA nanosheets as a wound dressing for partial-thickness burn wounds in mice. The PLLA nanosheets tightly adhered to the wound without any adhesive agents. Although wound infection with Pseudomonas aeruginosa in the controls significantly impaired reepithelialization of burn wounds, dressing with the PLLA nanosheet markedly protected against bacterial wound infection, thereby improving wound healing in the mice receiving partial-thickness burn injuries. The PLLA nanosheet also showed a potent barrier ability for protecting against bacterial penetration in vitro. The ultrathin PLLA nanosheet may be applied as a protective dressing to reduce environmental contamination of bacteria in a partial-thickness burn wound.
Division of Traumatology, Research Institute, National Defense Medical College, Tokorozawa, Japan.
Hiromi
H
Miyazaki
Manabu
M
Kinoshita
Akihiro
A
Saito
Toshinori
T
Fujie
Koki
K
Kabata
Etsuko
E
Hara
Satoshi
S
Ono
Shinji
S
Takeoka
Daizoh
D
Saitoh
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000494:pharmacology; D001458:Bandages; D002056:Burns / Q000382:microbiology / Q000473:pathology*; D002448:Cell Adhesion; D004195:Disease Models, Animal; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D053761:Nanocomposites / Q000627:therapeutic use; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D011552:Pseudomonas Infections / Q000382:microbiology / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000472:pathogenicity*; D014945:Wound Healing; D014946:Wound Infection / Q000209:etiology* / Q000382:microbiology / Q000517:prevention & control*
D000891:Anti-Infective Agents, Local; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)