Preparation and characterization of porous cross linked laccase aggregates for the decolorization of triphenyl methane and reactive dyes.
The production of porous cross-linked enzyme aggregates (p-CLEAs) is a simple and effective methodology for laccase immobilization. A three-phase partitioning technique was applied to co-precipitate laccase and starch, followed by cross-linking with glutaraldehyde and removal of starch by α-amylase to create pores in the CLEAs. Scanning electron microscopy revealed a very smooth spherical structure with numerous large pores. The half-life of free laccase at 55°C was calculated to be 1.3h, while p-CLEAs did not lose any activity even after 14 h. p-CLEAs also exhibited improved storage stability, catalytic efficiency and could be recycled 15 times with 60% loss of activity. The catalysts decolorized triphenylmethane and reactive dyes by 60-70% at initial dye concentrations of 2 and 0.5 g L(-1), respectively, without any mediators. These results suggest the potential of CLEA technology in waste-water treatment.
Department of Applied Science and Technology, Environmental Management Laboratory, A.C. Tech, Anna University, Chennai 600016, Tamil Nadu, India.
V
V
Vinoth Kumar
M P
MP
Prem Kumar
K V
KV
Thiruvenkadaravi
P
P
Baskaralingam
P
P
Senthil Kumar
S
S
Sivanesan
D001487:Basidiomycota / Q000201:enzymology*; D003116:Color; D004396:Coloring Agents / Q000737:chemistry* / Q000302:isolation & purification; D003432:Cross-Linking Reagents / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000138:chemical synthesis; D042845:Laccase / Q000138:chemical synthesis*; D016062:Porosity; D013730:Terphenyl Compounds / Q000737:chemistry* / Q000302:isolation & purification; D014320:Triphenylmethyl Compounds / Q000737:chemistry* / Q000302:isolation & purification
D004396:Coloring Agents; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D013730:Terphenyl Compounds; D014320:Triphenylmethyl Compounds; C046945:triphenylmethane; D042845:Laccase