Endothelial nitric oxide synthase (G894T) gene polymorphism in a random sample of the Egyptian population: comparison with myocardial infarction patients.
OBJECTIVE
The aim of this study was to detect endothelial nitric oxide synthase (eNOS) Glu298Asp gene variants in a random sample of the Egyptian population, compare it with those from other populations, and attempt to correlate these variants with serum levels of nitric oxide (NO). The association of eNOS genotypes or serum NO levels with the incidence of acute myocardial infarction (AMI) was also examined.


METHODS
One hundred one unrelated healthy subjects and 104 unrelated AMI patients were recruited randomly from the 57357 Hospital and intensive care units of El Demerdash Hospital and National Heart Institute, Cairo, Egypt. eNOS genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism. Serum NO was determined spectrophotometrically.


RESULTS
The genotype distribution of eNOS Glu298Asp polymorphism determined for our sample was 58.42% GG (wild type), 33.66% GT, and 7.92% TT genotypes while allele frequencies were 75.25% and 24.75% for G and T alleles, respectively. No significant association between serum NO and specific eNOS genotype could be detected. No significant correlation between eNOS genotype distribution or allele frequencies and the incidence of AMI was observed.


CONCLUSIONS
The present study demonstrated the predominance of the homozygous genotype GG over the heterozygous GT and homozygous TT in random samples of Egyptian population. It also showed the lack of association between eNOS genotypes and mean serum levels of NO, as well as the incidence of AMI.
Clinical Biochemistry Unit, Faculty of Pharmacy and Biotechnology, German University in Cairo, New Cairo City, Egypt. mohamed.gad@guc.edu.eg
Mohamed Z
MZ
Gad
Mohamed F
MF
Abdel Rahman
Ingy M
IM
Hashad
Sahar M
SM
Abdel-Maksoud
Nabil M
NM
Farag
Khaled
K
Abou-Aisha
D000293:Adolescent; D000328:Adult; D019943:Amino Acid Substitution; D004534:Egypt / Q000453:epidemiology; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*; D009569:Nitric Oxide / Q000097:blood / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D009569:Nitric Oxide; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III