[Simultaneous determination of five synthetic sweeteners in food by solid phase extraction-high performance liquid chromatography-evaporative light scattering detection].
A high performance liquid chromatographic method with evaporative light scattering detection (HPLC-ELSD) was developed for the simultaneous determination of five synthetic sweeteners (acesulfame-K, saccharin sodium, sodium cyclamate, sucralose and aspartame) in food. The sweeteners were extracted by 0.1% (v/v) formic acid buffer solution. The extract of sample was cleaned up and concentrated with solid phase extraction (SPE) cartridge. Then the sweeteners were separated on a C18 column (3 microm) using 0.1% (v/v) formic acid buffer (adjusted to pH = 3.5 with aqueous ammonia solution)-methanol (61: 39, v/v) as mobile phase, and finally detected by ELSD. The results showed that the reasonable linearity was achieved for all the analytes over the range of 30 - 1000 mg/L with the correlation coefficients (r) greater than 0.997. The recoveries for the five sweeteners ranged from 85.6% to 109.0% at three spiked concentrations with the relative standard deviations (RSDs) lower than 4.0%. The limits of detection (LODs, S/N = 3) were 2.5 mg/L for both acesulfame-K and sucralose, 3 mg/L for saccharin sodium, 10 mg/L for sodium cyclamate, and 5 mg/L for aspartame. The method is simple, sensitive and low cost, and has been successfully applied to the simultaneous determination of the five synthetic sweeteners in food.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Fang
F
Liu
Yan
Y
Wang
Yuhong
Y
Wang
Junyi
J
Zhou
Chao
C
Yan
D002851:Chromatography, High Pressure Liquid; D059022:Food Safety; D008027:Light; D064306:Non-Nutritive Sweeteners / Q000032:analysis*; D012542:Scattering, Radiation; D052616:Solid Phase Extraction
D064306:Non-Nutritive Sweeteners