Effects of the interaction between Na2Wo4-6-benzylaminopurine anionic chelate and rhodamine 6G on the resonance Rayleigh scattering and fluorescence spectra and their analytical applications.
In pH 4.99-6.06 Britton-Robinson (BR) buffer medium, 6-benzylaminopurine (6-BA) reacted with Na2WO4 to form 1:1 anionic chelate (6-BA·WO4)(2-), which further reacted with rhodamine 6G to form ternary ion complexes at room temperature. This resulted in a significant enhancement of resonance Rayleigh scattering (RRS) with a maximum RRS wavelength of 316 nm. Meanwhile, the fluorescence of the solution was quenched and excitation (λ ex) and emission (λ em) wavelengths of the fluorescence were 290 and 559 nm, respectively. Intensities of RRS enhancing (ΔIRRS ) and fluorescence quenching (ΔIF) were directly proportional to concentrations of 6-BA. As a result, RRS and fluorescence quenching for determination of trace amounts of 6-BA were developed. Under optimal conditions, linear ranges and detection limits of the two methods were 0.05-15.00 µg/mL and 8.2 ng/mL (RRS), 0.50-15.00 µg/mL and 17.0 ng/mL, respectively. It was found that the RRS method was superior to fluorescence quenching. The influence of these methods were investigated and results showed that RRS had good selectivity. RRS was applied to determine 6-BA in vegetable samples with satisfactory results. Furthermore, the reaction mechanisms of the ternary ion-association system are discussed. In addition, the polarization experiment revealed that the resonance light scattering (RLS) peak of Na2WO4-6-BA-R6G consisted mainly of depolarized resonance fluorescence and resonance scattering. It was speculated that light emission fluorescence energy (EL) transformed into resonance light scattering energy (ERLS), which was a key reason for enhancement of RRS.
School of Chemistry and Chemical Engineering, Southwest University, Chongqing Beibei, 400715, China.
Xiao-yan
XY
Li
Zhou
Z
Shang
He
H
Wei
Ji-dong
JD
Yang
D001593:Benzyl Compounds / Q000737:chemistry*; D002212:Capsicum / Q000737:chemistry*; D018553:Cucumis sativus / Q000737:chemistry*; D003583:Cytokinins / Q000737:chemistry*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D010936:Plant Extracts / Q000737:chemistry*; D011687:Purines / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods*
D001593:Benzyl Compounds; D003583:Cytokinins; D010936:Plant Extracts; D011687:Purines; D012235:Rhodamines; C026188:rhodamine 6G; C480551:benzylaminopurine