[Simultaneous determination of four components in sini tang by HPLC].
OBJECTIVE
To develop an HPLC method for determining four components in Sini Tan, benzoylmesaconine, liquiritin, glycyrrhizic acid and 6-gingerol.


METHODS
The Hypersil BDS column was adopted with gradient elution program at a flow rate of 1.0 mL x min(-1) and the detection wavelength of 235 nm.


RESULTS
Benzoylmesaconine, liquiritin, glycyrrhizic acid and 6-gingerol showed good separation, with the linear range of 0.006-0.12, 0.021-0.42, 0.012-0.24 and 0.018-0.36 g x L(-1), respectively. Their average recoveries were 99.3%, 96.9%, 100% and 100%, respectively; and RSD of the above four components were 1.5%, 0.6%, 1.3% and 2.1%, respectively.


CONCLUSIONS
The method is proved to be so easy and accurate and practical that it can be used to determine the four components in Sini Tang.
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Xiao
X
Liu
Linqian
L
Fan
Hao
H
Cai
Baochang
B
Cai
D000157:Aconitine / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000302:isolation & purification; D002396:Catechols / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005233:Fatty Alcohols / Q000032:analysis / Q000302:isolation & purification; D044950:Flavanones / Q000032:analysis / Q000302:isolation & purification; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification; D019695:Glycyrrhizic Acid / Q000032:analysis / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional; D015203:Reproducibility of Results
D000893:Anti-Inflammatory Agents; D002396:Catechols; D004365:Drugs, Chinese Herbal; D005233:Fatty Alcohols; D044950:Flavanones; D005960:Glucosides; C478756:sini tang; D019695:Glycyrrhizic Acid; C007845:gingerol; C085283:benzoylmesaconine; C512196:liquiritin; D000157:Aconitine