In vitro and in vivo protection provided by pinocembrin against lipopolysaccharide-induced inflammatory responses.
Pinocembrin or 5, 7-dihydroxyflavanone is a flavanone, a type of flavonoid. In the present study, we first assessed the anti-inflammatory effects of pinocembrin in RAW macrophage cells; and based on these effects, we investigated the therapeutic effects of pinocembrin in murine model of endotoxin-induced acute lung injury. We found that in vitro pretreatment with pinocembrin remarkably regulated the production of TNF-α, IL-1β, IL-6 and IL-10 via inhibiting the phosphorylation of IκBα, ERK1/2, JNK and p38MAPK. In the mouse model of LPS-induced acute lung injury, pinocembrin (20 or 50 mg/kg, i.p.) attenuated the development of pulmonary edema, histological severities, as well as neutrophil, lymphocyte and macrophage infiltration, which were increased by LPS administration. Additionally, TNF-α, IL-1β and IL-6 concentrations decreased significantly while the concentration of IL-10 was significantly increased after pinocembrin pretreatment. Our results also showed that pinocembrin attenuated LPS-induced lung injury through suppression of IκBα, JNK and p38MAPK activation. These findings suggest that pinocembrin may represent a novel candidate for the modulation of inflammatory responses.
Department of veterinary pharmacology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, People's Republic of China.
Lanan Wassy
LW
Soromou
Xiao
X
Chu
Lanxiang
L
Jiang
Miaomiao
M
Wei
Meixia
M
Huo
Na
N
Chen
Shuang
S
Guan
Xiaofeng
X
Yang
Chengzhen
C
Chen
Haihua
H
Feng
Xuming
X
Deng
D055371:Acute Lung Injury / Q000188:drug therapy*; D032429:Alpinia / Q000276:immunology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage* / Q000494:pharmacology; D002199:Capillary Permeability / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D019610:Cytoprotection; D044950:Flavanones / Q000008:administration & dosage* / Q000494:pharmacology; D007111:Immunity, Cellular / Q000187:drug effects; D008070:Lipopolysaccharides / Q000276:immunology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D044950:Flavanones; D008070:Lipopolysaccharides; D016328:NF-kappa B; C016063:pinocembrin