α-Ketoglutaric acid production by Yarrowia lipolytica and its regulation.
The yeast Yarrowia lipolytica VKM Y-2412 was selected as a prospective producer of α-ketoglutaric acid (KGA) from ethanol. The following peculiarities were found: (1) the intensive KGA production occurred only under the limitation of cell growth by thiamine and the excess of ethanol and nitrogen, (2) the production of KGA from ethanol required increased amount of zinc and iron ions, and (3) KGA production increased significantly with a high aeration at pH medium equal to 3.5. Under optimal conditions, the Y. lipolytica VKM Y-2412 produced up to 172 g l(-1) of KGA with the mass yield coefficient of 0.70 g g(-1).
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, Moscow region, 142290, Russia. kamzolova@rambler.ru
Svetlana V
SV
Kamzolova
Maria N
MN
Chiglintseva
Julia N
JN
Lunina
Igor G
IG
Morgunov
D000431:Ethanol / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D007501:Iron / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism; D013831:Thiamine / Q000378:metabolism; D025062:Yarrowia / Q000235:genetics* / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
D007656:Ketoglutaric Acids; D000431:Ethanol; D007501:Iron; D015032:Zinc; D009584:Nitrogen; D013831:Thiamine