Cotton gene expression profiles in resistant Gossypium hirsutum cv. Zhongzhimian KV1 responding to Verticillium dahliae strain V991 infection.
Verticillium wilt of cotton (Gossypium hirsutum) is a widespread and destructive disease that is caused by the soil-borne fungus pathogen Verticillium dahliae (V. dahliae). To study the molecular mechanism in wilt tolerance, suppression subtractive hybridization (SSH) and dot blot techniques were used to identify the specifically expressed genes in a superior wilt-resistant cotton cultivar (G. hirsutum cv. Zhongzhimian KV1) after inoculation with pathogen. cDNAs from the root tissues of Zhongzhimian KV1 inoculated with V. dahliae strain V991 or water mock were used to construct the libraries that contain 4800 clones. Based on the results from dot blot analysis, 147 clones were clearly induced by V. dahliae and selected from the SSH libraries for sequencing. A total of 92 up-regulated and 7 down-regulated non-redundant expressed sequences tags (ESTs) were identified as disease responsive genes and classified into 9 functional groups. Two important clues regarding wilt-resistant G. hirsutum were obtained from this study. One was Bet v 1 family; the other was UbI gene family that may play an important role in the defense reaction against Verticillium wilt. The result from real-time quantitative reverse transcription polymerase chain reaction showed that these genes were activated quickly and transiently after inoculation with V. dahliae.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China. zwwei@sohu.com
Wen-Wei
WW
Zhang
Gui-Liang
GL
Jian
Teng-Fei
TF
Jiang
Sheng-Zheng
SZ
Wang
Fang-Jun
FJ
Qi
Shi-Chang
SC
Xu
D060467:Disease Resistance / Q000235:genetics*; D020224:Expressed Sequence Tags; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D056726:Genetic Association Studies; D003368:Gossypium / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D054884:Host-Pathogen Interactions; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome; D020172:Verticillium / Q000502:physiology*