Spectrally encoded photoacoustic microscopy using a digital mirror device.
We have developed spectrally encoded photoacoustic microscopy using a digital mirror device for multi-wavelength tomography, which enables fast spectral imaging of optical absorption. The optical illumination wavelengths are multiplexed at a laser pulse repetition rate of ≈ 2 kHz. Liquid samples, whole blood, and blood vessels in mouse ears were imaged. Compared with internal wavelength tuning of a narrow-band laser, external wavelength tuning based on a digital mirror device improves the data acquisition speed of spectral photoacoustic microscopy. Compared with external wavelength scanning of a wide-band laser with the same pulse energy, spectral encoding improves the signal-to-noise ratio.
Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, 1 Brookings Drive, St. Louis, Missouri 63130, USA.
Yu
Y
Wang
Konstantin
K
Maslov
Lihong V
LV
Wang
D000818:Animals; D001808:Blood Vessels / Q000473:pathology; D002417:Cattle; D004396:Coloring Agents / Q000737:chemistry; D004423:Ear / Q000502:physiology; D004867:Equipment Design; D007834:Lasers; D008027:Light; D051379:Mice; D033401:Microscopy, Acoustic / Q000295:instrumentation / Q000379:methods*; D015233:Models, Statistical; D055095:Optics and Photonics / Q000379:methods; D061088:Photoacoustic Techniques / Q000295:instrumentation / Q000379:methods*; D059629:Signal-To-Noise Ratio; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D014465:Ultrasonics
D004396:Coloring Agents