A rigid network of long-range contacts increases thermostability in a mutant endoglucanase.
Thermodynamic stability of a protein at elevated temperatures is a key factor for thermostable enzymes to catalyze their specific reactions. Yet our understanding of biological determinants of thermostability is far from complete. Many different atomistic factors have been suggested as possible means for such proteins to preserve their activity at high temperatures. Among these factors are specific local interatomic interactions or enrichment of specific amino acid types. The case of glycosyl hydrolase family endoglucanase of Trichoderma reesei defies current hypotheses for thermostability because a single mutation far from the active site (A35 V) converts this mesostable protein into a thermostable protein without significant change in the protein structure. This substantial change in enzymatic activity cannot be explained on the basis of local intramolecular interactions alone. Here we present a more global view of the induced thermostability and show that the A35 V mutation affects the underlying structural rigidity of the whole protein via a number of long-range, non-local interactions. Our analysis of this structure reveals a precisely tuned, rigid network of atomic interactions. This cooperative, allosteric effect promotes the transformation of this mesostable protein into a thermostable one.
Department of Physics, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA. ajrader@iupui.edu
A J
AJ
Rader
Ragothaman M
RM
Yennamalli
Andrew K
AK
Harter
Taner Z
TZ
Sen
D019943:Amino Acid Substitution; D020134:Catalytic Domain; D002480:Cellulase / Q000737:chemistry* / Q000235:genetics; D003198:Computer Simulation; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D006860:Hydrogen Bonding; D008958:Models, Molecular; D050505:Mutant Proteins / Q000737:chemistry* / Q000235:genetics; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D014242:Trichoderma / Q000201:enzymology*
D005656:Fungal Proteins; D050505:Mutant Proteins; D002480:Cellulase