Time-lapse evaluation of human embryo development in single versus sequential culture media--a sibling oocyte study.
OBJECTIVE
To compare the dynamics of early development between embryos cultured in single and sequential media.


METHODS
Randomized, comparative study.


METHODS
Private IVF centre.


METHODS
A total of 446 metaphase II oocytes from 51 couples who underwent oocyte retrieval procedure for intracytoplasmic sperm injection. Forty-nine resulted in embryo transfer.


METHODS
Oocytes were split between single and sequential media produced by the same manufacturer and cultured in a time-lapse incubator.


METHODS
Morphokinetic parameters until the embryos reached the 5-cell stage (t5), utilization, clinical pregnancy and implantation rates.


RESULTS
Embryos cultured in single media were advanced from the first mitosis cycle and reached 2- to 5-cell stages earlier. There was not any difference between the durations for cell cycle two (cc2 = t3-t2) and s2 (t4-t3). The utilization, clinical pregnancy and implantation rates did not differ between groups. The proportion of cryopreserved day 6 embryos to two pronuclei oocytes was significantly higher in sequential than in single media.


CONCLUSIONS
Morphokinetics of embryo development vary between single and sequential culture media at least until the 5-cell stage. The overall clinical and embryological parameters remain similar regardless of the culture system.
Fulya IVF Clinic, Bahceci Health Group, Hakki Yeten cd 11, Terrace Fulya, Istanbul, Turkey. nadirc@superonline.com
Haydar Nadir
HN
Ciray
Turan
T
Aksoy
Cihan
C
Goktas
Bilgen
B
Ozturk
Mustafa
M
Bahceci
D000328:Adult; D015925:Cryopreservation; D003470:Culture Media / Q000737:chemistry*; D046149:Embryo Culture Techniques / Q000379:methods*; D010064:Embryo Implantation; D004624:Embryo Transfer / Q000379:methods; D004622:Embryo, Mammalian / Q000166:cytology*; D047108:Embryonic Development; D005260:Female; D006801:Humans; D007185:Incubators; D008677:Metaphase; D054315:Oocyte Retrieval / Q000379:methods; D009865:Oocytes / Q000166:cytology* / Q000254:growth & development; D011247:Pregnancy; D011897:Random Allocation; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D059008:Time-Lapse Imaging / Q000379:methods*
D003470:Culture Media