Anti-proliferative effect of an analogue of the LL-37 peptide in the             colon cancer derived cell line HCT116 p53+/+ and p53-/-.
Antimicrobial peptides of the cathelicidin family are found in many mammalian             species, and are focused on various effects other than antimicrobial action. In             this study, we evaluated the anti-proliferative effect of an analogue peptide,             FF/CAP18, derived from an endogenous cathelicidin family member against the colon             cancer cell line HCT116. FF/CAP18 significantly decreased the proliferation of             HCT116 cells in a dose-dependent fashion. Furthermore, the treatment of HCT116             with FF/CAP18 caused loss of mitochondrial membrane potential, and resulted in             the immunoreactivity to the single-strand DNA antibody, suggesting the early stage             of apoptosis. Interestingly, the anti-proliferative effect of FF/CAP18 was constant             regardless of the genotype of p53 (wild-type and p53 mutant type HCT116 cells).             Therefore, the signaling pathway of p53 is not involved in the growth suppression             effect of the cathelicidin analogue peptide. These results indicate that the treatment             of certain types of cancer cells with FF/CAP18 may increase the sensitivity of             the chemotherapeutic reagents, which might relate to the reduction of the side             effects.
Laboratory of Animal Microbiology, Graduate School of Agricultural                     Science, Tohoku University, Sendai 981-8555, Japan.
Kengo
K
Kuroda
Tomokazu
T
Fukuda
Hiroshi
H
Yoneyama
Masafumi
M
Katayama
Hiroshi
H
Isogai
Kazuhiko
K
Okumura
Emiko
E
Isogai
D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D054804:Cathelicidins / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms; D053938:DNA Fragmentation / Q000187:drug effects; D004277:DNA, Single-Stranded / Q000378:metabolism; D045325:HCT116 Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000235:genetics*
D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; D054804:Cathelicidins; D004277:DNA, Single-Stranded; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53