[Effect of montelukast on morphological changes in neurons after ischemic injury].
To determine the effect of montelukast, a cysteinyl leukotriene receptor 1 antagonist, on morphological changes in rat neurons after ischemic injury.



The in vivo ischemia injury was induced by oxygen-glucose deprivation (OGD) for 2 h and reperfusion (R) for 24 h (OGD/R) in rat neurons primary culture and mixed cortex culture. In the presence or absence of various concentrations of montelukast, neuron number, area of neuron, number of neuritis per neuron, branch number of primary neuritis and primary neurite length were determined for evaluating morphological changes in neurons.



OGD/R significantly reduced neuron number, and altered neuron morphology. In cortical neuron cultures, montelukast (0.0001-1 μmol/L) attenuated OGD/R-induced reduction in neuron number, and inhibited OGD/R-induced increase in branch number of primary neuritis. In the mixed cultures, montelukast (0.0001-0.1 μmol/L) increased the primary neurite length, and reduced number of neuritis and branch number of primary neurite after OGD/R.



Montelukast has a protective effect on ischemic injury in neurons.
Department of Pharmacology, Zhejiang University School of Medicine, Hangzhou 310058, China.
Xin-xin
XX
Wang
Xia-yan
XY
Zhang
Xue-qin
XQ
Huang
Shu-ying
SY
Yu
Rui
R
Zhao
San-hua
SH
Fang
Yun-bi
YB
Lu
Wei-ping
WP
Zhang
Er-qing
EQ
Wei
D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003521:Cyclopropanes; D005947:Glucose / Q000494:pharmacology; D020024:Leukotriene Antagonists / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D018696:Neuroprotective Agents / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013440:Sulfides
D000085:Acetates; D003521:Cyclopropanes; D020024:Leukotriene Antagonists; D018696:Neuroprotective Agents; D011804:Quinolines; D013440:Sulfides; D005947:Glucose; C093875:montelukast