PCR for diagnosis of male Trichomonas vaginalis infection with chronic prostatitis and urethritis.
The aim of this study was to assess the usefulness of PCR for diagnosis of Trichomonas vaginalis infection among male patients with chronic recurrent prostatitis and urethritis. Between June 2001 and December 2003, a total of 33 patients visited the Department of Urology, Hanyang University Guri Hospital and were examined for T. vaginalis infection by PCR and culture in TYM medium. For the PCR, we used primers based on a repetitive sequence cloned from T. vaginalis (TV-E650). Voided bladder urine (VB1 and VB3) was sampled from 33 men with symptoms of lower urinary tract infection (urethral charge, residual urine sensation, and frequency). Culture failed to detect any T. vaginalis infection whereas PCR identified 7 cases of trichomoniasis (21.2%). Five of the 7 cases had been diagnosed with prostatitis and 2 with urethritis. PCR for the 5 prostatitis cases yielded a positive 330 bp band from bothVB1 and VB3, whereas positive results were only obtained from VB1 for the 2 urethritis patients. We showed that the PCR method could detect T. vaginalis when there was only 1 T. vaginalis cell per PCR mixture. Our results strongly support the usefulness of PCR on urine samples for detecting T. vaginalis in chronic prostatitis and urethritis patients.
Prime Urology Clinic, Gwangmyeong 423-807, Korea.
Jong Jin
JJ
Lee
Hong Sang
HS
Moon
Tchun Yong
TY
Lee
Hwan Sik
HS
Hwang
Myoung-Hee
MH
Ahn
Jae-Sook
JS
Ryu
D000328:Adult; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011472:Prostatitis / Q000175:diagnosis / Q000469:parasitology; D056910:Republic of Korea; D014245:Trichomonas Infections / Q000175:diagnosis* / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000235:genetics / Q000302:isolation & purification*; D014526:Urethritis / Q000175:diagnosis / Q000469:parasitology
D017931:DNA Primers