A MAP kinase gene, Clk1, is required for conidiation and pathogenicity in the phytopathogenic fungus Curvularia lunata.
Mitogen-activated protein kinase (MAPK) cascades are highly conserved signal transduction pathways, which play a wide variety of important roles in extracellular signal transduction. The first MAPK gene of the maize pathogen Curvularia lunata, Clk1, was isolated via a PCR-based approach with a primer pair designed on the basis of conserved regions of known MAPKs. Southern blot analysis showed that the gene existed in the genome as a single copy. The predicted amino acid sequence (352 amino acids) was highly homologous with MAP kinases of other phytopathogenic fungi. Flanking regions of Clk1 were obtained through RACE and genomic walking technology. To understand the role of Clk1 in C. lunata, targeted gene disruption was adopted to construct Clk1 mutants. It was found that mutants lacking functional domain of Clk1 were not able to produce conidia but tended to form a few special chlamydospore-shaped structures. Clk1 mutants grew slower in adverse environments (at 24°C), produced less cell degrading enzymes (CWDEs) than the wild type, and they were almost unable to infect maize leaves via artificial wounds.
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, P.R. China.
Shi Gang
SG
Gao
Fei Hong
FH
Zhou
Tong
T
Liu
Ying Ying
YY
Li
Jie
J
Chen
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000235:genetics / Q000502:physiology; D014774:Virulence; D003313:Zea mays / Q000382:microbiology*
D005656:Fungal Proteins; D020928:Mitogen-Activated Protein Kinases