[Comparative study on the content of chlorogenic acid in Solanum torvum from different origin, different harvest time and different part].
OBJECTIVE
To develop a HPLC method for determination of chlorogenic acid in Solanum torvum and provide a scientific basis for evaluating the quality and reasonable utilization of the herb.


METHODS
HPLC was used to quantitatively determine the chlorogenic acid content in Solanum torvum from different origin, different harvest time and different part. The assay was performed on a Agela Promosil C18 (4.6 mm x 250 mm, 5 microm) column and eluted with a mobile phase consisted of acetonitrile -0.1% phosphoric acid solution at a flow rate of 1.0 ml/min. The column temperature was set at 35 degrees C. The detection wavelength was 327 nm.


RESULTS
The calibration curve was linear within the range of 0.1000 - 200.0 microg/mL (r = 0.9999). The average recoveries were 99.8% (RSD = 0.71%). The contents of chlorogenic acid in Solanum torvum were different from different origin, different harvest time and different part.


CONCLUSIONS
The method is reliable, accurate and specific. It can be used for quality control of Solanum torvum and reasonable utilization of the herb.
Zhongshan Campus of Guangdong Pharmaceutical University, Zhongshan 528458, China. heyang626@163.com
Yang
Y
He
Hong-Bo
HB
Zhou
Jian-Xiong
JX
Liu
Hui-Shan
HS
Li
Ping
P
Li
Zhi-Jian
ZJ
Fang
D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results; D012621:Seasons; D032322:Solanum / Q000737:chemistry* / Q000254:growth & development; D012997:Solvents / Q000737:chemistry
D012997:Solvents; D002726:Chlorogenic Acid