Generation of animals allowing the conditional inactivation of the Pax4 gene.
Pax4 belongs to the paired-box family of transcription factors. The analysis of loss- and gain-of-function mutant animals revealed that this factor plays a crucial role in the endocrine pancreas. Indeed, Pax4 is required for the genesis of insulin-producing beta-cells. Remarkably, the sole misexpression of Pax4 in glucagon-expressing cells is able to induce their regeneration, endow these with beta-cell features, and thereby counter chemically induced diabetes. However, the function of Pax4 in adult endocrine cells remains unclear. Herein, we report the generation of Pax4 conditional knockout mice that will allow the analysis of Pax4 function in mature beta-cells, as well as in the adult central nervous system.
Department of Molecular Cell Biology, Max-Planck Institute for Biophysical Chemistry, Am Fassberg, 37077 Göttingen, Germany.
Simon
S
Kordowich
Palle
P
Serup
Patrick
P
Collombat
Ahmed
A
Mansouri
D000818:Animals; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D018398:Homeodomain Proteins / Q000502:physiology*; D007124:Immunoenzyme Techniques; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism*; D019426:Integrases / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D051761:Paired Box Transcription Factors / Q000502:physiology*; D010187:Pancreatic Hormones / Q000378:metabolism*
D018398:Homeodomain Proteins; D051761:Paired Box Transcription Factors; D010187:Pancreatic Hormones; C110218:Pax4 protein, mouse; C045073:Cre recombinase; D019426:Integrases