[Determination of plasma protein binding rate of isopropylidene-shikimic acid].
OBJECTIVE
To study the plasma protein binding rate of isopropylidene-shikimic acid.


METHODS
The ultrafiltration was employed to determine the plasma protein binding rate of isopropylidene-shikimic acid. The plasma concentrations of isopropylidene-shikimic acid were measured by HPLC.


RESULTS
The plasma protein binding rate of isopropylidene-shikimic acid with dog plasma at the concentration of 0.3, 0.15 g x L(-1) and 0.5 mg x L(-1) were (4.36 +/- 0.02)%, (4.12 +/- 0.19)% and (2.23 +/- 0.59)%, respectively. While the plasma protein binding rate of isopropylidene-shikimic acid with normal human plasma at the above concentrations were (11.23 +/- 0.01)%, (10.06 +/- 0.69)% and (9.72 +/- 0.59)%, respectively.


CONCLUSIONS
The binding rate of isopropylidene-shikimic acid with plasma protein is low.
Beijing University of Chinese Medicine, Beijing 100102, China. doris7629@126.com
Hui
H
Kong
Hongxia
H
Xing
Jin
J
Liu
Lei
L
Yan
Longfei
L
Lin
Jian
J
Ni
D000475:Alkenes / Q000737:chemistry*; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006801:Humans; D011485:Protein Binding; D012765:Shikimic Acid / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity
D000475:Alkenes; D001798:Blood Proteins; D012765:Shikimic Acid; C013658:propylene