Bone tissue, cellular, and molecular responses to titanium implants treated by anodic spark deposition.
A myriad of titanium (Ti) surface modifications has been proposed to hasten the osseointegration. In this context, the aim of this study was to perform histomorphometric, cellular, and molecular analyses of the bone tissue grown in close contact with Ti implants treated by anodic spark deposition (ASD-AK). Acid-etched (AE) Ti implants either untreated or submitted to ASD-AK were placed into dog mandibles and retrieved at 3 and 8 weeks. It was noticed that both implants, AE and ASD-AK, were osseointegrated at 3 and 8 weeks. Histomorphometric analysis showed differences between treatments only for bone-to-implant contact, being higher on AE implants. Although not backed by histomorphometric results, gene expression of key bone markers was higher for bone grown in close contact with ASD-AK and for cells harvested from these fragments and cultured until subconfluence. Cell proliferation at days 7 and 10 and alkaline phosphatase activity at day 10 was higher on AE surfaces. No statistical significant difference was noticed for extracellular matrix mineralization at 17 days. Our results have shown that the Ti fixtures treated by ASD-AK allowed in vivo osseointegration and induced higher expression of key markers of osteoblast phenotype, suggesting that this surface treatment could be considered to produce implants for clinical applications.
Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil.
Alexander T
AT
Sverzut
Gustavo C
GC
de Albuquerque
Grasiele E
GE
Crippa
Roberto
R
Chiesa
Cinzia
C
Della Valle
Paulo T
PT
de Oliveira
Marcio M
MM
Beloti
Adalberto L
AL
Rosa
D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D017267:Dental Prosthesis Design; D004285:Dogs; D004566:Electrodes; D008334:Mandible / Q000601:surgery* / Q000648:ultrastructure; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism
D020099:Coated Materials, Biocompatible; D014025:Titanium