Controllable stabilization of poly(N-isopropylacrylamide)-based microgel films through biomimetic mineralization of calcium carbonate.
Two types of thermoresponsive microgels, poly(N-isopropylacrylamide) (PNIPAM) microgels and poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAMAC) microgels were synthesized and used as templates for the mineralization of amorphous calcium carbonate (ACC) by diffusion of CO(2) vapor under ambient conditions. Thermosensitive PNIPAM/CaCO(3) hybrid macroscopic hydrogels and micrometer-sized PNIPAMAC/CaCO(3) hybrid microgels were controllably obtained and different mineralization mechanistic processes were proposed. The impact of the loaded CaCO(3) on the size, morphology, stability, and thermosensitivity of the microgels was also analyzed. PNIPAM/CaCO(3) hybrid macrogels had a slight decrease in thermoresponsive phase transition temperature, while PNIPAMAC/CaCO(3) hybrid microgels showed a clear increase in phase transition temperature. The difference reflected different amount and location of ACC in the gel network, causing different interactions with polymer chains. The PNIPAMAC/CaCO(3) microgels formed stable monolayer films on bare silica wafers and glass coverslips upon drying. The microgel films could facilitate the attachment and growth of 3T3 fibroblast cells and their subsequent detachment upon temperature drop from 37 °C to the ambient condition around 20 °C, thus, offering a convenient procedure for cell harvesting.
Centre for Bioengineering and Biotechnology, Qingdao Economic Development Zone, Qingdao, China.
Yongqing
Y
Xia
Yanfeng
Y
Gu
Xuan
X
Zhou
Hai
H
Xu
Xiubo
X
Zhao
Mohammed
M
Yaseen
Jian Ren
JR
Lu
D000178:Acrylamides / Q000138:chemical synthesis* / Q000737:chemistry; D000180:Acrylic Resins; D000818:Animals; D032701:Biomimetics; D002113:Calcification, Physiologic; D002119:Calcium Carbonate / Q000737:chemistry*; D002448:Cell Adhesion; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000648:ultrastructure; D010316:Particle Size; D044367:Phase Transition; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering; D044366:Transition Temperature; D014961:X-Ray Diffraction
D000178:Acrylamides; D000180:Acrylic Resins; D020100:Hydrogels; C090629:N-isopropylacrylamide-acrylic acid copolymer; D011108:Polymers; C052970:poly-N-isopropylacrylamide; D002119:Calcium Carbonate