A BODIPY-based colorimetric and fluorometric chemosensor for Hg(II) ions and its application to living cell imaging.
A new monostyryl boron dipyrromethene derivative (MS1) appended with two triazole units indicates the presence of Hg(2+) among other metal ions with high selectivity by color change and red emission. Upon Hg(2+) binding, the absorption band of MS1 is blue-shifted by 29 nm due to the inhibition of the intramolecular charge transfer from the nitrogen to the BODIPY, resulting in a color change from blue to purple. Significant fluorescence enhancement is observed with MS1 in the presence of Hg(2+); the metal ions Ag(+), Ca(2+), Cd(2+), Co(2+), Cu(2+), Fe(2+), Fe(3+), K(+), Mg(2+), Mn(2+), Ni(2+), Pb(2+), and Zn(2+) cause only minor changes in the fluorescence of the system. The apparent association constant (K(a)) of Hg(2+) binding in MS1 is found to be 1.864 × 10(5) M(-1). In addition, fluorescence microscopy experiments show that MS1 can be used as a fluorescent probe for detecting Hg(2+) in living cells.
Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, Republic of China.
Mani
M
Vedamalai
Shu-Pao
SP
Wu
D001896:Boron Compounds / Q000737:chemistry*; D002413:Cations, Divalent / Q000032:analysis; D002470:Cell Survival; D003124:Colorimetry / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D008628:Mercury / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods; D012680:Sensitivity and Specificity
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D008628:Mercury