The effects of intravenous anesthetics on mouse embryonic fibroblast viability and proliferation.
OBJECTIVE
The aim of this study is to evaluate the cytotoxic and antiproliferating effects of intravenous anesthetics on an mouse fibroblast in vitro cell culture system.


METHODS
The cells were exposed to the usual clinical plasma concentration of intravenous anesthetics, i.e., midazolam (0.15 μg/ml), propofol (2 μg/ml), remifentanil (2 μg/ml), thiopental (10 μg/ml), for 4, 8, or 24 h. Cell proliferation (n = 6 for each) under intravenous anesthetics was analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Cytotoxicity (n = 6 for each) of intravenous anesthetics was investigated using a LIVE/DEAD viability assay kit.


RESULTS
Intravenous anesthetic exposure time did not affect the proliferation rate of mouse fibroblasts. The cytotoxicity of intravenous anesthetics did not differ in accordance with exposure time.


CONCLUSIONS
Our results showed that intravenous anesthetics may not affect mouse fibroblast proliferation and viability.
Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan, Seoul, Korea.
Yoon Ji
YJ
Choi
Se Keun
SK
Jeon
Jungjoo
J
Kim
Yongdoo
Y
Park
Seung Zhoo
SZ
Yoon
Kyung
K
Sun
D018686:Anesthetics, Intravenous / Q000494:pharmacology* / Q000633:toxicity*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects*; D051379:Mice; D008874:Midazolam / Q000009:adverse effects / Q000494:pharmacology; D010880:Piperidines / Q000009:adverse effects / Q000494:pharmacology; D015742:Propofol / Q000009:adverse effects / Q000494:pharmacology; D000077208:Remifentanil; D013874:Thiopental / Q000009:adverse effects / Q000494:pharmacology
D018686:Anesthetics, Intravenous; D010880:Piperidines; D013874:Thiopental; D000077208:Remifentanil; D008874:Midazolam; D015742:Propofol