High fat diet-induced glucose intolerance impairs myocardial function, but not myocardial perfusion during hyperaemia: a pilot study.
BACKGROUND
Glucose intolerance is a major health problem and is associated with increased risk of progression to type 2 diabetes mellitus and cardiovascular disease. However, whether glucose intolerance is related to impaired myocardial perfusion is not known. The purpose of the present study was to study the effect of diet-induced glucose intolerance on myocardial function and perfusion during baseline and pharmacological induced hyperaemia.


METHODS
Male Wistar rats were randomly exposed to a high fat diet (HFD) or control diet (CD) (n = 8 per group). After 4 weeks, rats underwent an oral glucose tolerance test. Subsequently, rats underwent (contrast) echocardiography to determine myocardial function and perfusion during baseline and dipyridamole-induced hyperaemia (20 mg/kg for 10 min).


RESULTS
Four weeks of HFD feeding resulted in glucose intolerance compared to CD-feeding. Contractile function as represented by fractional shortening was not altered in HFD-fed rats compared to CD-fed rats under baseline conditions. However, dipyridamole increased fractional shortening in CD-fed rats, but not in HFD-fed rats. Basal myocardial perfusion, as measured by estimate of perfusion, was similar in CD- and HFD-fed rats, whereas dipyridamole increased estimate of perfusion in CD-fed rats, but not in HFD-fed rats. However, flow reserve was not different between CD- and HFD-fed rats.


CONCLUSIONS
Diet-induced glucose intolerance is associated with impaired myocardial function during conditions of hyperaemia, but myocardial perfusion is maintained. These findings may result in new insights into the effect of glucose intolerance on myocardial function and perfusion during hyperaemia.
Department of Anesthesiology, VU University Medical Center, Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands. c.vandenbrom@vumc.nl
Charissa E
CE
van den Brom
Carolien S E
CS
Bulte
B Margreet
BM
Kloeze
Stephan A
SA
Loer
Christa
C
Boer
R Arthur
RA
Bouwman
D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects*; D004176:Dipyridamole / Q000494:pharmacology; D004195:Disease Models, Animal; D018149:Glucose Intolerance / Q000097:blood / Q000150:complications* / Q000503:physiopathology; D005951:Glucose Tolerance Test; D006331:Heart Diseases / Q000097:blood / Q000000981:diagnostic imaging / Q000209:etiology* / Q000503:physiopathology; D006940:Hyperemia / Q000000981:diagnostic imaging / Q000503:physiopathology*; D008297:Male; D009200:Myocardial Contraction; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014463:Ultrasonography; D014665:Vasodilator Agents / Q000494:pharmacology
D014665:Vasodilator Agents; D004176:Dipyridamole