Rapid recovery of tissue hypoxia by cotransplantation of endothelial cells.
Disruption of blood vessels caused by a spinal cord injury leads to tissue hypoxia. This hypoxic condition reduces the survival of transplanted stem cells, consequentially decreasing the effectiveness of stem cell therapy. In this study, we investigated the correlation between angiogenesis and the survival of transplanted neural stem cells in a spinal cord injury model. Hypoxia-specific luciferase-expressing neural stem cells (EpoSV-Luc NSC) were used as a tool for the detection of hypoxia caused by a spinal cord injury. In vivo, angiogenesis by cotransplantation of endothelial cells quickly recovered tissue hypoxia caused by a spinal cord injury. As a result, cotransplantation of endothelial cells improved the survival of neural stem cells transplanted into the injured spinal cord.
Department of Neurosurgery, Spine and Spinal Cord Institute, College of Medicine, Yonsei University, Seoul, Korea.
Jinsoo
J
Oh
Keung Nyun
KN
Kim
Do Heum
DH
Yoon
Seong Rok
SR
Han
Dong Ah
DA
Shin
Yoon
Y
Ha
D000818:Animals; D061307:Human Umbilical Vein Endothelial Cells / Q000637:transplantation*; D000860:Hypoxia / Q000628:therapy; D018919:Neovascularization, Physiologic; D058953:Neural Stem Cells / Q000637:transplantation*; D051381:Rats; D013119:Spinal Cord Injuries / Q000628:therapy*; D033581:Stem Cell Transplantation / Q000379:methods