The CCR4-NOT complex is implicated in the viability of aneuploid yeasts.
To identify the genes required to sustain aneuploid viability, we screened a deletion library of non-essential genes in the fission yeast Schizosaccharomyces pombe, in which most types of aneuploidy are eventually lethal to the cell. Aneuploids remain viable for a period of time and can form colonies by reducing the extent of the aneuploidy. We hypothesized that a reduction in colony formation efficiency could be used to screen for gene deletions that compromise aneuploid viability. Deletion mutants were used to measure the effects on the viability of spores derived from triploid meiosis and from a chromosome instability mutant. We found that the CCR4-NOT complex, an evolutionarily conserved general regulator of mRNA turnover, and other related factors, including poly(A)-specific nuclease for mRNA decay, are involved in aneuploid viability. Defective mutations in CCR4-NOT complex components in the distantly related yeast Saccharomyces cerevisiae also affected the viability of spores produced from triploid cells, suggesting that this complex has a conserved role in aneuploids. In addition, our findings suggest that the genes required for homologous recombination repair are important for aneuploid viability.
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
Yoshie
Y
Tange
Atsushi
A
Kurabayashi
Bunshiro
B
Goto
Kwang-Lae
KL
Hoe
Dong-Uk
DU
Kim
Han-Oh
HO
Park
Jacqueline
J
Hayles
Yuji
Y
Chikashige
Chihiro
C
Tsutumi
Yasushi
Y
Hiraoka
Fumiaki
F
Yamao
Paul
P
Nurse
Osami
O
Niwa
D000782:Aneuploidy; D002470:Cell Survival / Q000235:genetics*; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D059765:Homologous Recombination / Q000235:genetics; D008540:Meiosis; D020871:RNA Stability / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development; D012568:Schizosaccharomyces / Q000235:genetics / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D013170:Spores / Q000235:genetics / Q000254:growth & development
D005095:Exoribonucleases; D016601:RNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; C519160:Ccr4 protein, S pombe