Evidence of DNA double strand breaks formation in Escherichia coli bacteria exposed to alpha particles of different LET assessed by the SOS response.
Ionizing radiation produces a plethora of lesion upon DNA which sometimes is generated among a relatively small region due to clustered energy deposition events, the so called locally multiply damaged sites that could change to DSB. Such clustered damages are more likely to occur in high LET radiation exposures. The effect of alpha particles of different LET was evaluated on the bacterium Escherichia coli either by survival properties or the SOS response activity. Alpha radiation and LET distribution was controlled by means of Nuclear Track Detectors. The results suggest that alpha particles produce two types of lesion: lethal lesions and SOS inducing-mutagenic, a proportion that varies depending on the LET values. The SOS response as a sensitive parameter to assess RBE is mentioned.
Departamento de Biología, Instituto Nacional de Investigaciones Nucleares, Carretera Federal México-Toluca, La Marquesa, Mexico. jorge.serment@inin.gob.mx
Jorge
J
Serment-Guerrero
Matilde
M
Breña-Valle
Magdalena
M
Aguilar-Moreno
Miguel
M
Balcázar
D000512:Alpha Particles; D003037:Cobalt Radioisotopes; D053903:DNA Breaks, Double-Stranded; D004926:Escherichia coli / Q000528:radiation effects*; D018499:Linear Energy Transfer; D013014:SOS Response, Genetics
D003037:Cobalt Radioisotopes