Biodiversity and quantification of functional bacteria in completely autotrophic nitrogen-removal over nitrite (CANON) process.
The research was conducted to investigate the microbial diversity and population with the different concentration of NH(4)(+)-N in a biofilm reactor filled with volcanic filter for completely autotrophic nitrogen-removal over nitrite (CANON) process. The reactor had an excellent performance with the decreasing of NH(4)(+)-N concentration from 400 to 200 mg L(-1) while NH(4)(+)-N removal loading reduced at the NH(4)(+)-N concentration of 100 mg L(-1). Biodiversity analysis indicated that Nitrosomonas related aerobic ammonia oxidizing bacteria (AOB) and Planctomycetales-like anaerobic ammonia oxidizing (anammox) bacteria were dominant functional bacteria. Despite the different influent NH(4)(+)-N concentration, anammox bacteria had a low and stable biodiversity, which was not the same to AOB. With the concentration reduction of influent NH(4)(+)-N, the estimates of total bacteria population ranged between 2.29×10(11) and 1.44×10(12) copies mg(-1) total DNA, and the quantity of AOB decreased while anammox bacteria kept stable. The population of Nitrospira increased and little Nitrobacter was detected during the experiment.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Tao
T
Liu
Dong
D
Li
Huiping
H
Zeng
Xiangkun
X
Li
Taotao
T
Zeng
Xiaoyan
X
Chang
Yan'an
Y
Cai
Jie
J
Zhang
D000641:Ammonia / Q000378:metabolism; D052818:Autotrophic Processes; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D058645:Denaturing Gradient Gel Electrophoresis; D006863:Hydrogen-Ion Concentration; D009573:Nitrites / Q000302:isolation & purification*; D009584:Nitrogen / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D012995:Solubility
D009573:Nitrites; D000641:Ammonia; D009584:Nitrogen; D010100:Oxygen