Uptake of well-defined, highly glycosylated, pentafluorostyrene-based polymers and nanoparticles by human hepatocellular carcinoma cells.
Chain length, size, composition, surface charge, and other properties of polymeric materials affect their recognition and uptake by cells and must be optimized to deliver polymers selectively to their target. However, it is often not possible to precisely modify selected properties without changing other parameters. To overcome these difficulties, well-defined poly(pentafluorostyrene)-based polymers are prepared that can be grafted via thiol/para-fluorine "click" reaction with 1-thio-β-D-glucose and 1-thio-β-D-galactose. Fluorescence microscopy and flow cytometry show that nanoparticles are taken up by HepG2 cells to a higher degree than the respective water-soluble polymers, and that internalization of both galactosylated homo- and nanoprecipitated block copolymers is enhanced.
Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich-Schiller-University Jena, Jena, Germany.
Krzysztof
K
Babiuch
David
D
Pretzel
Tatiana
T
Tolstik
Antje
A
Vollrath
Sarmiza
S
Stanca
Franziska
F
Foertsch
C Remzi
CR
Becer
Michael
M
Gottschaldt
Christoph
C
Biskup
Ulrich S
US
Schubert
D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D005434:Flow Cytometry; D006031:Glycosylation; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D011108:Polymers / Q000378:metabolism*; D011137:Polystyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D011108:Polymers; D011137:Polystyrenes