Elevated expressions of GH/IGF axis genes in triploid crucian carp.
Growth hormone (GH), growth hormone receptor (GHR) and insulin-like growth factor 1 (IGF-1) are pivotal signaling factors of the GH/IGF axis, which plays a crucial role in regulating growth in vertebrates. In this study, GH, GHR and IGF-1 cDNAs were cloned from triploid and tetraploid crucian carp. In addition, mRNA expression levels were characterized in diploid red crucian carp, triploids and tetraploids. Reverse transcriptase PCR indicated that GH genes were only expressed in the pituitary, while GHR and IGF-1 were widely expressed in all tested tissues. Real-time PCR study of different seasonal profiles showed that triploids had significantly higher expression of the studied genes during both the prespawning and the spawning season. Although different temperatures (22, 26 and 30°C) showed no significant effects on GH, GHR and IGF-1 mRNA expression in either diploids or triploids, triploids had higher expression levels than diploids at each temperature. After 1 week of fasting, the expression of all studied genes was reduced in both diploids and triploids, while the expressions levels were higher in triploids than in diploids. These results suggest that the elevated expression of GH/IGF axis genes in triploids plays a crucial role in the faster growth rate of triploids.
Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Huan
H
Zhong
Yi
Y
Zhou
Shaojun
S
Liu
Min
M
Tao
Yu
Y
Long
Zhen
Z
Liu
Chun
C
Zhang
Wei
W
Duan
Jie
J
Hu
Can
C
Song
Yun
Y
Liu
D000818:Animals; D002347:Carps / Q000235:genetics* / Q000254:growth & development; D013006:Growth Hormone / Q000235:genetics*; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D011123:Polyploidy; D011986:Receptors, Somatotropin / Q000235:genetics*
D011986:Receptors, Somatotropin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone