Effect of medium composition on the in vitro culture of bovine pre-antral follicles: morphology and viability do not guarantee functionality.
Summary This study investigated the effect of three different culture media (α minimum essential medium (α-MEM), McCoy or TCM199 during the in vitro culture (IVC) of bovine isolated pre-antral follicles. Pre-antral follicles greater than 150 μm in size were isolated and cultured for 0 (control), 8 or 16 days in one of the abovementioned culture media. Follicles were evaluated for survival, growth and antrum formation at days 8 and 16. The results showed that TCM199 was the most suitable medium to preserve follicular viability and ultrastructure, resulting in the highest rates of antrum formation. In conclusion, TCM199 promotes the in vitro development of isolated pre-antral follicles without hampering follicular functionality by sustaining in vitro growth and antrum formation.
Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, Fortaleza, CE, Brasil. rafael.rossetto@hotmail.com
Rafael
R
Rossetto
Márcia Viviane Alves
MV
Saraiva
Regiane Rodrigues
RR
dos Santos
Cleidson Manoel Gomes
CM
da Silva
Luciana Rocha
LR
Faustino
Roberta Nogueira
RN
Chaves
Ivina Rocha
IR
Brito
Giovanna Quintino
GQ
Rodrigues
Isadora Machado Teixeira
IM
Lima
Mariana Aragão Matos
MA
Donato
Christina Alves
CA
Peixoto
José Ricardo
JR
de Figueiredo
D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques; D009930:Organic Chemicals / Q000494:pharmacology; D006080:Ovarian Follicle / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure
D003470:Culture Media; D009930:Organic Chemicals; C420642:alpha minimal essential medium