Time-lapse analysis of laser phase shift for noninvasive discrimination of human normal cells and malignant tumor cells.
Time-lapse analysis of phase shift using phase-shifting laser microscopy (PLM) revealed that the laser phase shifts of primary normal human prostate epithelial cells (PRECs) and human prostatic carcinoma epithelial cell line (PC-3) cells during the mitotic phase were markedly higher than those in the interphase. The phase shift of PC-3 cells during the interphase was markedly lower than that of PRECs throughout the cell cycle. In conclusion, it was proposed that adherent PC-3 cancer cells could be noninvasively discriminated from normal adherent PRECs by the periodical measurement of phase shift using PLM during culture.
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kita-ku, Sapporo 060-8628, Japan. takagi-m@eng.hokudai.ac.jp
Mutsumi
M
Takagi
Yuhei
Y
Shibaki
D000818:Animals; D016466:CHO Cells; D002453:Cell Cycle; D002455:Cell Division; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004847:Epithelial Cells / Q000166:cytology / Q000473:pathology; D006801:Humans; D007700:Kinetics; D007834:Lasers; D008297:Male; D018613:Microscopy, Confocal; D011467:Prostate / Q000166:cytology / Q000473:pathology; D011471:Prostatic Neoplasms / Q000473:pathology*