New sub-diffraction-limit microscopy technique: dual-point illumination AND-gate microscopy on nanodiamonds (DIAMOND).
We introduce a new, easily implementable sub-diffraction-limit microscopy technique utilizing the optical AND-gate property of fluorescent nanodiamond (FND). We demonstrate that when FND is illuminated by two spatially-offset lights of different wavelengths, emission comes only from the region of their overlap, which is used to reduce the effective point spread function from ~300 nm to ~130 nm in lateral plane, well below the diffraction limit.
Department of Biophysics and Chemical biology, Seoul National University, Seoul 151-747, South Korea.
Jiwoong
J
Kwon
Youngbin
Y
Lim
Jiwon
J
Jung
Seung Keun
SK
Kim
D003460:Crystallization; D008029:Lighting; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000379:methods*; D058612:Nanodiamonds / Q000737:chemistry*
D058612:Nanodiamonds