Two new monoclonal antibodies for biochemical and flow cytometric analyses of human interferon regulatory factor-3 activation, turnover, and depletion.
Interferon regulatory factor-3 (IRF-3) is a master transcription factor that drives the host intracellular innate immune response to virus infection. The importance of IRF-3 in innate immune responses is highlighted by the fact that pathogenic viruses have developed strategies for antagonism of IRF-3. Several tools exist for evaluation of viral regulation of IRF-3 activation and function, but high-quality monoclonal antibodies that mark the differential activation states of human IRF-3 are lacking. To study IRF-3 activation, turnover, and depletion in a high-throughput manner in the context of virus infection, we have developed two new monoclonal antibodies to human IRF-3. These antibodies detect IRF-3 in virus-infected cells in a wide variety of assays and provide a new tool to study virus-host interactions and innate immune signaling.
Department of Global Health, University of Washington School of Medicine, Seattle, WA 98195-7650, USA. arustagi@uw.edu
Arjun
A
Rustagi
Brian P
BP
Doehle
M Juliana
MJ
McElrath
Michael
M
Gale
D000595:Amino Acid Sequence; D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D005434:Flow Cytometry / Q000379:methods*; D006367:HeLa Cells; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D050838:Interferon Regulatory Factor-3 / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data
D061067:Antibodies, Monoclonal, Humanized; C494232:IRF3 protein, human; D050838:Interferon Regulatory Factor-3