Albusin B modulates lipid metabolism and increases antioxidant defense in broiler chickens by a proteomic approach.
BACKGROUND
The present study was designed to investigate the effect of albusin B on lipid metabolism and antioxidant defense in broiler chickens by a proteomic approach. The bacteriocin, albusin B of Ruminococcus albus 7, expressed by yeast was applied in this study. Three dietary treatments, consisting of the basal diet (control), basal diet + albusin B (2.5 g kg⁻¹), and basal diet + nosiheptide (2.5 mg kg⁻¹) were randomly fed to 90 broiler chickens from 1 to 35 days of age, respectively. After 35 days of supplementation, the growth performance, lipid metabolism and antioxidant proteins in the jejunum and liver, intestinal protein profile, and plasma lipid profile were analyzed.


RESULTS
Broilers with albusin B supplementation had greater body weight than the control broilers. Compared with the control broilers, lower triglyceride and higher high-density lipoprotein concentration in the blood were observed in both broilers with albusin B and nosiheptide supplementation. In addition, albusin B suppressed the mRNA expression of fatty acid binding protein 2 and ATP binding cassette transporter G 5 in the jejunum. In the jejunal protein profiles, four antioxidant proteins were upregulated by albusin B and nosiheptide treatments. The jejunal antioxidant gene expression had a concordant pattern. Hepatic genes related to lipid metabolism, 3-hydroxy-3-methyl-glutaryl CoA reductase, and superoxide dismutase were upregulated by albusin B supplementation.


CONCLUSIONS
Albusin B supplementation modulated lipid metabolism and activated systemic antioxidant defense, which might partially contribute to the performance of broiler chickens.
Department of Animal Science, Chinese Culture University, Taipei, Taiwan.
Han-Tsung
HT
Wang
Yi-Hung
YH
Li
I-Pin
IP
Chou
Ya-Hui
YH
Hsieh
Bao-Ji
BJ
Chen
Ching-Yi
CY
Chen
D018528:ATP-Binding Cassette Transporters / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001430:Bacteriocins / Q000235:genetics / Q000378:metabolism*; D002645:Chickens / Q000097:blood / Q000254:growth & development / Q000378:metabolism*; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D015536:Down-Regulation; D004790:Enzyme Induction; D050556:Fatty Acid-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000662:veterinary; D006903:Hydroxymethylglutaryl CoA Reductases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000960:Hypolipidemic Agents / Q000378:metabolism; D007583:Jejunum / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D008297:Male; D011994:Recombinant Proteins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D013844:Thiazoles / Q000378:metabolism; D015430:Weight Gain
D018528:ATP-Binding Cassette Transporters; D000975:Antioxidants; D001430:Bacteriocins; D050556:Fatty Acid-Binding Proteins; D000960:Hypolipidemic Agents; D011994:Recombinant Proteins; D013844:Thiazoles; C581354:albusin B, Ruminococcus albus; D006903:Hydroxymethylglutaryl CoA Reductases; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; C014134:nosiheptide