Cystoisospora spp. from dogs in China and phylogenetic analysis of its 18S and ITS1 gene.
Cystoisospora spp. oocysts isolated from dog feces in Changchun, China were morphologically similar to those of Cystoisospora ohioensis and Cystoisospora sp. 1-MM recently isolated from dogs in Japanese. Sequencing results of the 18S subunit RNA gene from isolates in the present study were compared to other Cystoisospora spp. and the results suggested that Cystoisospora spp. from dogs in Changchun was homologous to C. ohioensis and Cystoisospora sp. 1-MM. Phylogenetic analysis of the 18S rRNA sequences showed that the Cystoisospora sp. ChangChun 1 and Cystoisospora sp. ChangChun 2 were nested in a clade with other Cystoisospora spp., including C. ohioensis, Cystoisospora belli, Cystoisospora suis, Isospora sp. Harbin/01/08 and C. orlovi,. Cystoisospora sp. ChangChun 2 was confirmed as C. ohioensis, and the other isolate was in a separate clade but the genetic relationship was relatively close to C. suis after analysis of the ITS-1gene.
College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun 130062, China.
Pengfei
P
He
Jianhua
J
Li
Pengtao
P
Gong
Jingui
J
Huang
Xichen
X
Zhang
D000818:Animals; D002681:China; D003048:Coccidiosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics*; D004283:Dog Diseases / Q000175:diagnosis / Q000469:parasitology*; D004285:Dogs; D005243:Feces / Q000469:parasitology; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D021745:Sarcocystidae / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S