Natural oils enhance IL-10 and IFN-γ production by human PBMCs cultured with Malassezia furfur.
BACKGROUND
Malassezia furfur is a lipophilic yeast that causes skin disease.


OBJECTIVE
To evaluate the level of IL-10, IFN-γ and IL-12P70 in co-incubation of peripheral blood mononuclear cells (PBMCs) with M. furfur grown in the presence of some different types of natural oils.


METHODS
PBMCs were obtained from blood samples of normal volunteers. M. furfur was cultured in different culture media containing almond oil, fish oil, walnut oil, full-fat milk, and a fat-free medium; and the yeasts grown were harvested and used for co-incubation with PBMCs in vitro. The IFN-γ, IL-10, and IL-12P70 levels were measured at different time intervals using ELISA methods.


RESULTS
Generally, IFN-γ and IL-10 levels in the co-incubation of yeasts with walnut oil group (WOG) and fish oil group (FOG) were higher than those in the almond oil group (AOG) and full-fat milk group (FFMG). Although the IL-12P70 was higher in groups such as AOG, FOG, and WOG; the increase was not statistically significant.


CONCLUSIONS
The results demonstrated that the type of fat used by M. furfur in the culture media can influence the immune response and increasesIFN-γ and IL-10 levels in an early time point of the culture system.
Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran, e-mail: rahmany191@gmail.com.
Mohammad Ali
MA
Rezaee
Yousef
Y
Motaharinia
Werya
W
Hosseini
Ali
A
Jalili
Ahmad
A
Rashidi
Bita
B
Mosavi
Ghasem
G
Zamini
Mohammad Reza
MR
Rahmani
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005395:Fish Oils / Q000494:pharmacology; D005399:Fishes / Q000276:immunology; D006801:Humans; D031324:Juglans / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008290:Malassezia / Q000276:immunology*; D008892:Milk / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology; D014010:Tinea Versicolor / Q000276:immunology*
D016207:Cytokines; D005395:Fish Oils; D010938:Plant Oils; C068582:almond oil