A novel soluble β-1,3-D-glucan salecan reduces adiposity and improves glucose tolerance in high-fat diet-fed mice.
Salecan is a recently identified water-soluble viscous extracellular β-1,3-D-glucan polysaccharide from an Agrobacterium species. It is a high-molecular-mass polymer (about 2 × 10⁶ Da) and composed of a linear chain of glucosyl residues linked through a repeat unit of seven β-(1,3) and two α-(1,3) glucosidic bonds. In the present study, we examined the effects of dietary Salecan fed at 2 and 5 % in a high-fat diet (64 % energy) in C57BL/6J mice. After 6 weeks, mice fed 2 and 5 % Salecan had significantly lower body weight, fat mass and percentage of body fat mass compared with those fed a high-fat cellulose (control) diet. Both the Salecan groups significantly and dose-dependently improved glucose tolerance, with a 9 and 26 % reduction of glucose AUC, respectively. Liver and adipose tissue weights were also significantly decreased by the Salecan treatment. Supplementation with 5 % Salecan led to lower serum TAG, total cholesterol (TC) and HDL-cholesterol (52, 18 and 19 %, respectively) and lower hepatic TAG by 56 % and TC by 22 % compared with the high-fat cellulose control group. Dietary Salecan intake caused an obvious elevation of fat in the faeces. Supplementation with Salecan disturbed bile acid-promoted emulsification and reduced the size of emulsion droplets in vitro. These results indicate that Salecan decreases fat absorption, improves glucose tolerance and has biologically important, dose-related effects on reducing high-fat diet-induced obesity.
Center for Molecular Metabolism, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China.
Ying
Y
Zhang
Lin
L
Xia
Wenqiang
W
Pang
Tao
T
Wang
Peng
P
Chen
Bin
B
Zhu
Jianfa
J
Zhang
D050154:Adiposity; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D048429:Cell Size; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000032:analysis / Q000378:metabolism; D019587:Dietary Supplements; D045703:Emulsifying Agents / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use; D005243:Feces / Q000737:chemistry; D005260:Female; D018149:Glucose Intolerance / Q000209:etiology / Q000517:prevention & control*; D007408:Intestinal Absorption; D050152:Intra-Abdominal Fat / Q000473:pathology; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009929:Organ Size; D012995:Solubility; D015430:Weight Gain; D047071:beta-Glucans / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*
D019440:Anti-Obesity Agents; D004041:Dietary Fats; D045703:Emulsifying Agents; D047071:beta-Glucans; C577623:salecan