Ethanol production from lignocellulosic biomass by recombinant Escherichia coli strain FBR5.
Lignocellulosic biomass, upon pretreatment and enzymatic hydrolysis, generates a mixture of hexose and pentose sugars such as glucose, xylose, arabinose and galactose. While Escherichia coli utilizes all these sugars it lacks the ability to produce ethanol from them. Recombinant ethanologenic E. coli strains have been created with a goal to produce ethanol from both hexose and pentose sugars. Herein, we review the current state of the art on the production of ethanol from lignocellulosic hydrolyzates by an ethanologenic recombinant E. coli strain (FBR5). The bacterium is stable without antibiotics and can tolerate ethanol up to 50 gL(-1). It produces up to 45 g ethanol per L and has the potential to be used for industrial production of ethanol from lignocellulosic hydrolyzates.
Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL, USA. Badal.Saha@ars.usda.gov
Badal
B
Saha
Michael A
MA
Cotta
D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D001089:Arabinose / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D060847:Metabolic Engineering; D009154:Mutation; D011767:Pyruvate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D014994:Xylose / Q000378:metabolism; D017355:Zymomonas / Q000737:chemistry
D001426:Bacterial Proteins; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D014994:Xylose; D001089:Arabinose; D000426:Alcohol Dehydrogenase; D011767:Pyruvate Decarboxylase; D005947:Glucose; D005690:Galactose