Dentin as a suitable bone substitute comparable to ß-TCP--an experimental study in mice.
Microvascular supply is of fundamental importance to the survival and integration of grafting. Since the autogenous bone is still the gold standard for osseous augmentation, the aim of this study was to analyze the initial osseous, angiogenic and inflammatory response and subsequent osseointegration after implantation of dentin and beta-tricalcium phosphate (ß-TCP) scaffolds into the calvaria chamber of balb/c mice comparing with bone. The vascularisation of perforated implants of dentin (n=8), ß-TCP (n=8) and isogenic calvarial bone (n=8) displaying pores similar in size and structure was analyzed in vivo using intravital fluorescence microscopy. In additional animals (n=24) the osseointegration of dentin, ß-TCP and bone implants was assessed by fluorochrome sequential labelling of growing bone for up to 12 weeks. Animals without implants served as controls. Intravital fluorescence microscopy revealed that implantation of bone substitutes caused an only mild inflammatory response. Comparable to isogenic bone both dentin and ß-TCP scaffolds were found nearly completely vascularized by day 22 and osseointegrated within 12 weeks. In conclusion, dentin and ß-TCP scaffolds are similar to isogenic bone in terms of inflammatory and neovascularization response, highlighting their potential utility in regeneration of bone defects.
Department of Oral and Maxillofacial Surgery, Hannover Medical School, Hannover, Germany. Bormann.Kai-Hendrik@mh-hannover.de
Kai-Hendrik
KH
Bormann
Maria Mercedes
MM
Suarez-Cunqueiro
Branko
B
Sinikovic
Andreas
A
Kampmann
Constantin
C
von See
Frank
F
Tavassol
Thomas
T
Binger
Meike
M
Winkler
Nils-Claudius
NC
Gellrich
Martin
M
Rücker
D000818:Animals; D018786:Bone Substitutes / Q000009:adverse effects / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000276:immunology / Q000494:pharmacology*; D003804:Dentin / Q000276:immunology / Q000637:transplantation*; D005260:Female; D007249:Inflammation / Q000276:immunology; D036904:Leukocyte Rolling / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D018919:Neovascularization, Physiologic / Q000187:drug effects; D016348:Osseointegration / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D012886:Skull / Q000187:drug effects* / Q000276:immunology / Q000637:transplantation*; D013997:Time Factors; D054457:Tissue Scaffolds
D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate