Effect of Pru p 3 on dendritic cell maturation and T-lymphocyte proliferation in peach allergic patients.
BACKGROUND
Pru p 3 is the major peach allergen and the most frequent cause of food allergy in adults in the Mediterranean area. Although its allergenicity is well characterized, its ability to generate a T-cell response is not completely known.


OBJECTIVE
To investigate the influence of Pru p 3 allergen on dendritic cell (DC) maturation and specific T-cell response (T(H)1/T(H)2) in peach allergic patients.


METHODS
Peach allergic patients (n = 11) and tolerant controls (n = 14) were included in the study. Monocyte-derived DC maturation after incubation with Pru p 3 was evaluated by the increase of maturational markers (CD80, CD86, and CD83) by flow cytometry. Lymphocyte proliferation was evaluated by coculturing monocyte-derived DCs and 5,6-carboxyfluorescein diacetate N-succinimidyl ester-stained lymphocytes with different concentrations of Pru p 3 (25, 10, and 1 μg/mL) by flow cytometry and cytokine production.


RESULTS
Pru p 3 induced a significant increase in the CD80, CD86, and CD83 expression on stimulated DCs from patients compared with controls. The lymphocyte proliferative response after Pru p 3 stimulation was also significantly higher along with an increase in interleukin 8 in patients compared with tolerant controls.


CONCLUSIONS
Pru p 3 allergen induces changes in DC maturational status mainly in peach allergic patients. An increase in lymphocyte proliferative response accompanied with a different cytokine pattern was also observed compared with healthy controls.
Research Laboratory for Allergic Diseases, Carlos Haya Hospital, Málaga, Spain.
Enrique
E
Gómez
Araceli
A
Díaz-Perales
Leticia
L
Tordesillas
Inmaculada
I
Doña
María J
MJ
Torres
Ana B
AB
Blázquez
Francisca
F
Gómez
Miguel
M
Blanca
Cristobalina
C
Mayorga
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology* / Q000378:metabolism; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D052179:Antigens, Plant / Q000276:immunology; D018122:B7-1 Antigen / Q000276:immunology / Q000378:metabolism; D051940:B7-2 Antigen / Q000276:immunology / Q000378:metabolism; D016022:Case-Control Studies; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005452:Fluoresceins; D005512:Food Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology / Q000378:metabolism; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D010940:Plant Proteins; D027861:Prunus / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D058408:Th1-Th2 Balance; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D000097802:CD83 Antigen
D000485:Allergens; D015703:Antigens, CD; D052179:Antigens, Plant; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D005452:Fluoresceins; D007136:Immunoglobulins; D016209:Interleukin-8; D008562:Membrane Glycoproteins; D010940:Plant Proteins; D000097802:CD83 Antigen; C508606:CXCL8 protein, human; C463181:Pru p 3 allergen; C093616:6-carboxyfluorescein diacetate