Partial antiviral activities of the Asn631 chicken Mx against newcastle disease virus and vesicular stomatitis virus.
Conflicting data existed for the antiviral potential of the chicken Mx protein and the importance of the Asn631 polymorphism in determination of the antiviral activity. In this study we modified the chicken Mx cDNA from the Ser631 to Asn631 genotype and transfected them into COS-I cells, chicken embryonic fibroblast (CEF) or NIH 3T3 cells. The Mx protein was mainly located at the cytoplasm. The transfected cell cultures were challenged with newcastle disease virus (NDV) or vesicular stomatitis virus (VSV), cytopathic affect (CPE) inhibition assay showed that the times for development of visible and full CPE were significantly postponed by the Asn631 cDNA transfection at 48 h transfection, but not by the Ser631 cDNA transfection. Viral titration assay showed that the virus titers were significantly reduced before 72 h postinfection. CEF cells was incubated by the cell lysates extracted from the COS-I cells transfected with pcDNA-Mx/Asn631, could resist and delayed NDV infection. These data suggested the importance of the Asn631 polymorphism of the chicken Mx in determination of the antiviral activities against NDV and VSV at early stage of viral infection, which were relatively weak and not sufficient to inhibit the viral replication at late stage of viral infection.
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China. yubcli@yzu.edu.cn
Bichun
B
Li
Dezhi
D
Fu
Yani
Y
Zhang
Qi
Q
Xu
Ligang
L
Ni
Guobin
G
Chang
Mengmeng
M
Zheng
Bo
B
Gao
Huaichang
H
Sun
Guohong
G
Chen
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D019556:COS Cells; D002645:Chickens; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D018076:DNA, Complementary; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D015870:Gene Expression; D051379:Mice; D009154:Mutation; D064547:Myxovirus Resistance Proteins; D041681:NIH 3T3 Cells; D009522:Newcastle disease virus / Q000187:drug effects*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D014162:Transfection; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects*
D000998:Antiviral Agents; D018076:DNA, Complementary; C579070:Mx1 protein, mouse; D064547:Myxovirus Resistance Proteins; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins