Integration of a bacterial β-carotene ketolase gene into the Mucor circinelloides genome by the Agrobacterium tumefaciens-mediated transformation method.
Plasmids introduced in Mucor circinelloides (and most transformable Mucorales) tend to replicate autonomously, and hardly ever integrate in the genome. This is critical if we want to express exogenous genes, because plasmids are easily lost during vegetative growth, and the ratio of plasmid molecules/nuclei is invariably low. Linearized molecules of DNA have been used to get their genomic integration but the transformation efficiency drops extremely. We have developed and highly optimized an efficient Agrobacterium-mediated transformation system for M. circinelloides to facilitate the integration of transforming DNA in the genome of the recipient strain that could also be used for other Mucorales.
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary. pappt@bio.u-szeged.hu
Tamás
T
Papp
Arpád
A
Csernetics
Ildikó
I
Nyilasi
Csaba
C
Vágvölgyi
Enrique A
EA
Iturriaga
D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D016681:Genome, Fungal / Q000235:genetics*; D009089:Mucor / Q000235:genetics*; D010105:Oxygenases / Q000235:genetics*; D010230:Paracoccus / Q000201:enzymology / Q000235:genetics*; D058432:Sporangia / Q000235:genetics; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D010105:Oxygenases; C093259:beta-carotene ketolase