Proteome alterations in primary human alveolar macrophages in response to influenza A virus infection.
To obtain a global picture of how alveolar macrophages respond to influenza A virus (IAV) infection, we used a quantitative proteomics method to systematically examine protein expression in the IAV-infected primary human alveolar macrophages. Of the 1214 proteins identified, 43 were significantly up-regulated and 63 significantly down-regulated at >95% confidence. The expression of an array of interferon (IFN)-induced proteins was significantly increased in the IAV-infected macrophages. The protein with the greatest expression increase was ISG15, an IFN-induced protein that has been shown to play an important role in antiviral defense. Concomitantly, quantitative real-time PCR analysis revealed that the gene expression of type I IFNs increased substantially following virus infection. Our results are consistent with the notion that type I IFNs play a vital role in the response of human alveolar macrophages to IAV infection. In addition to the IFN-mediated responses, inflammatory response, apoptosis, and redox state rebalancing appeared also to be major pathways that were affected by IAV infection. Furthermore, our data suggest that alveolar macrophages may play a crucial role in regenerating alveolar epithelium during IAV infection.
Department of Biological Sciences, University of Arkansas, Fayetteville, Arkansas 72701, United States.
Lin
L
Liu
Jianhong
J
Zhou
Yimeng
Y
Wang
Robert J
RJ
Mason
Cornelius Joel
CJ
Funk
Yuchun
Y
Du
D002478:Cells, Cultured; D015870:Gene Expression; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology / Q000502:physiology*; D007251:Influenza, Human / Q000276:immunology / Q000378:metabolism*; D007370:Interferon Type I / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D061251:Primary Cell Culture; D060066:Protein Interaction Maps; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism
D007370:Interferon Type I; D020543:Proteome; D014764:Viral Proteins