Behavior-related pauses in simple-spike activity of mouse Purkinje cells are linked to spike rate modulation.
Purkinje cells (PCs) in the mammalian cerebellum express high-frequency spontaneous activity with average spike rates between 30 and 200 Hz. Cerebellar nuclear (CN) neurons receive converging input from many PCs, resulting in a continuous barrage of inhibitory inputs. It has been hypothesized that pauses in PC activity trigger increases in CN spiking activity. A prediction derived from this hypothesis is that pauses in PC simple-spike activity represent relevant behavioral or sensory events. Here, we asked whether pauses in the simple-spike activity of PCs related to either fluid licking or respiration, play a special role in representing information about behavior. Both behaviors are widely represented in cerebellar PC simple-spike activity. We recorded PC activity in the vermis and lobus simplex of head-fixed mice while monitoring licking and respiratory behavior. Using cross-correlation and Granger causality analysis, we examined whether short interspike intervals (ISIs) had a different temporal relationship to behavior than long ISIs or pauses. Behavior-related simple-spike pauses occurred during low-rate simple-spike activity in both licking- and breathing-related PCs. Granger causality analysis revealed causal relationships between simple-spike pauses and behavior. However, the same results were obtained from an analysis of surrogate spike trains with gamma ISI distributions constructed to match rate modulations of behavior-related Purkinje cells. Our results therefore suggest that the occurrence of pauses in simple-spike activity does not represent additional information about behavioral or sensory events that goes beyond the simple-spike rate modulations.
Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA.
Ying
Y
Cao
Selva K
SK
Maran
Mukesh
M
Dhamala
Dieter
D
Jaeger
Detlef H
DH
Heck
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D015984:Causality; D002525:Cerebellar Cortex / Q000502:physiology; D002531:Cerebellum / Q000502:physiology; D003627:Data Interpretation, Statistical; D055724:Electrophysiological Phenomena; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011689:Purkinje Cells / Q000502:physiology*; D012119:Respiration