Photonic crystal slabs for surface contrast enhancement in microscopy of transparent objects.
In optical microscopy the contrast of transparent objects achieved with conventional methods is often not satisfactory, for example for the automated recognition of cells. In this paper we present a nano-optical label-free approach for contrast enhancement based on photonic crystal slabs (PCS) as the specimen holder. Quasi-guided modes inside these structures cause an intrinsic color of the PCS, which strongly depends on the wavelength and the quality factor of the optical mode. Objects on the surface of the PCS experience a significant color and intensity contrast enhancement, as they change properties of the optical modes.
Institute of Electrical and Information Engineering, Christian-Albrechts-Universität zu Kiel, D-24143 Kiel, Germany. yn@tf.uni-kiel.de
Yousef
Y
Nazirizadeh
Tim
T
Becker
Julia
J
Reverey
Christine
C
Selhuber-Unkel
Daniel H
DH
Rapoport
Uli
U
Lemmer
Martina
M
Gerken
D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation*; D017785:Photons; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000295:instrumentation*