Inducible biosynthetic nanoscaffolds as recruitment platforms for detecting molecular target interactions inside living cells.
We present a novel phenotypic readout using inducible, biosynthetic nanoscaffolds to directly visualize dynamic molecular interactions within living cells at the single-cell level with high sensitivity and selectivity. Labeled ferritin is used to form biological nanoparticles inside cells. Specific supramolecular assembly of ferritin-derived nanoparticles induces highly clustered nanoscaffolds. These inducible biosynthetic nanoscaffolds are used as the artificial recruitment/redistribution platform for monitoring interactions of a small molecule with its target protein(s) inside living cells.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea.
Sangkyu
S
Lee
Jae-Seok
JS
Ha
Seung-Goo
SG
Lee
Tae K
TK
Kim
D005293:Ferritins / Q000737:chemistry; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles; D054457:Tissue Scaffolds
D005456:Fluorescent Dyes; D005293:Ferritins