[A simple method for quantification of modified low-density lipoproteins].
A simple method for quantification of modified low-density lipoproteins (mLDL) in the blood serum containing 8.9% polyvinylpyrrolidone solution 12600 +/- 2700 has been developed. The results show that 10 min incubation of serum in a buffer containing 8.9% PVP leads to complete aggregation mLDL. Atherogenicity of aggregated mLDLs is experimentally confirmed by two independent tests (mLDLs bind and activate the complement system of a guinea pig (pro-inflammatory effect) and cause platelet hyperaggregation (thrombogenic effect)). The proposed method is simple and involves only two steps: mixing the serum with a solution of PVP and recording the turbidity. The method allows to register the presence of mLDL directly in serum without its prior fractionation.
B B
BB
Shoĭbonov
V Iu
VIu
Baronets
L F
LF
Panchenko
A A
AA
Pal'tsyn
A A
AA
Kubatiev
D000818:Animals; D003167:Complement Activation; D003168:Complement Fixation Tests / Q000379:methods; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D010974:Platelet Aggregation; D010979:Platelet Function Tests / Q000379:methods; D011205:Povidone / Q000031:analogs & derivatives / Q000737:chemistry; D044967:Serum / Q000737:chemistry
D008077:Lipoproteins, LDL; C077842:polyvinylpolypyrrolidone; D011205:Povidone