Polymorphism of insulin-like growth factor 1 gene and its association with litter size in Small Tail Han sheep.
The insulin-like growth factor 1 (IGF1) gene was studied as a candidate gene for high prolificacy in sheep. Polymorphisms of 5' regulatory region and all four exons of IGF1 gene were detected in Small Tail Han (n = 277), Hu (n = 58), Texel (n = 48) and Dorset (n = 46) sheep by PCR-RFLP and PCR-SSCP analysis. A microsatellite polymorphic site and a restriction fragment length polymorphism were shown in the 5' regulatory region of IGF1 gene. The ewes with genotype 123/123 bp had 0.81 (P < 0.05) or 1.03 (P < 0.01) lambs more than those with genotype 125/125 bp or 125/127 bp, the ewes with genotype 123/125 bp had 0.46 (P < 0.05) or 0.68 (P < 0.01) lambs more than those with genotype 125/125 bp or 125/127 bp. In addition, there were two mutations (C1511G and A1513G) in 5' regulatory region of IGF1 gene. The ewes with genotype BB or AB had 0.96 (P < 0.05) or 0.38 (P < 0.05) lambs more than those with genotype AA, but there were no significant differences between BB and AB genotypes (P > 0.05) in Small Tail Han sheep. These results preliminarily indicated that these polymorphisms of IGF1 gene could be used in molecular marker-assisted selection for sheep breeding programs.
Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
J N
JN
He
B Y
BY
Zhang
M X
MX
Chu
P Q
PQ
Wang
T
T
Feng
G L
GL
Cao
R
R
Di
L
L
Fang
D W
DW
Huang
Q Q
QQ
Tang
N
N
Li
D024506:5' Flanking Region; D000818:Animals; D001483:Base Sequence; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008098:Litter Size / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000235:genetics*
D007334:Insulin-Like Growth Factor I