[Application of high performance liquid chromatography-evaporative light scattering detection in determination of water-soluble sugars and sorbitol in tobacco flavourings and casings].
A simultaneous determination method of water-soluble sugars (rhamnose, xylose, fructose, glucose, sucrose, maltose) and sorbitol in tobacco flavourings and casings based on high performance liquid chromatography-evaporative light-scattering detection (HPLC-ELSD) was established. The analytes were extracted with ultrasonic assisted extraction into water and cleaned-up by Isolute ENV + solid phase extraction (SPE) cartridge, and then separated on Prevail Carbohydrate ES column (250 mm x 4.6 mm, 5 microm) with acetonitrile and water (75: 25, v/v) as mobile phase. The temperature of the drift tube in ELSD was 79 degrees C and the flow rate of carrier gas of nitrogen was 2.0 L/min. The calibration curves were plotted on the double logarithmic scales with the mass concentrations of analytes in the range of about 0.06 - 1.2 g/L. The limits of detection (LODs) were in the range of 12 - 26 mg/L. And the spiked recoveries in real samples were 88% - 109%. Based on the double logarithmic calibration of analytes, experiments were carried out to indicate that the physical and chemical characteristics of analytes, the retention behaviors on column and the elution ability of mobile phase can all influence the response of ELSD.
Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou 450001, China. binhu74@hotmail.com
Bin
B
Hu
Sheng
S
Wang
Fuwei
F
Xie
Huimin
H
Liu
D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005421:Flavoring Agents / Q000032:analysis*; D008027:Light; D012542:Scattering, Radiation; D013012:Sorbitol / Q000032:analysis*; D014026:Nicotiana / Q000737:chemistry*
D002241:Carbohydrates; D005421:Flavoring Agents; D013012:Sorbitol