A chromogenic cephalosporin for β-lactamase inhibitor screening assays.
Production of β-lactamases is the primary mechanism of antibiotic resistance employed by gram-negative pathogens. Chromogenic β-lactams are important reagents for detection and assay of β-lactamases, but limited commercial availability and exorbitant pricing of these compounds are prohibitive. Here we describe a straightforward synthesis of a chromogenic cephalosporin for β-lactamase assay that gives an overall yield of 74%. On hydrolysis, its λ(max) undergoes a bathochromic shift that is easy to see and measure spectrophotometrically with a Δε(442 nm) of 14,500 cm⁻¹ M⁻¹. This compound was shown to be a substrate for a variety of β-lactamases.
Sopharmia, Christopher S. "Kit" Bond Science and Technology Incubator, Missouri Western State University, Saint Joseph, MO 64507, USA.
Sophia
S
Yu
Amy
A
Vosbeek
Katherine
K
Corbella
Jonathan
J
Severson
Jacob
J
Schesser
Larry D
LD
Sutton
D002511:Cephalosporins / Q000737:chemistry / Q000494:pharmacology*; D002863:Chromogenic Compounds / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004791:Enzyme Inhibitors / Q000032:analysis* / Q000494:pharmacology*; D006868:Hydrolysis / Q000187:drug effects; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000378:metabolism
D002511:Cephalosporins; D002863:Chromogenic Compounds; D004791:Enzyme Inhibitors; D065093:beta-Lactamase Inhibitors; C000605808:chromacef; D001618:beta-Lactamases