[Protective effects and anticoagulation effect of polysaccharides from the sea cucumber on acute incomplete cerebral ischemia in rats].
OBJECTIVE
To investigate the protective effect and anticoagulation effect of polysaccharides from the sea cucumber (PSU) on acute incomplete cerebral ischemia (AICI).


METHODS
Adult SD rats were randomly divided into 5 group (n = 12): sham operation group, model group, low-dose (30 mg/(kg x d)), middle-dose (60 mg/(kg x d)) and high-dose (120 mg/(kg x d)) groups. The cerebral ischemia rat model was established by permanently ligating the common carotid arteries on both sides of rats. We observed the change of behavior disturbance, brain water content, the levels of serum C-reactive protein (CRP) as well as the effects on prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen(FIB).


RESULTS
Due to the use of polysaccharides, the behavioral disturbance was improved, brain water content and the levels of serum CRP were significantly decreased. Also PSU significantly prolonged APTT, TT and reduced the content of FIB (P < 0.05).


CONCLUSIONS
PSU has protective effect on acute incomplete cerebral ischemia injured rats and significant anticoagulation effect.
Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China.
Xin
X
Jiang
Jing
J
Xu
Xiu-Rong
XR
Su
Yan-Yan
YY
Li
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology / Q000627:therapeutic use; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D011134:Polysaccharides / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012616:Sea Cucumbers; D013918:Thrombin Time
D000925:Anticoagulants; D011134:Polysaccharides