Effects of ionomycin on egg activation and early development in starfish.
Ionomycin is a Ca(2+)-selective ionophore that is widely used to increase intracellular Ca(2+) levels in cell biology laboratories. It is also occasionally used to activate eggs in the clinics practicing in vitro fertilization. However, neither the precise molecular action of ionomycin nor its secondary effects on the eggs' structure and function is well known. In this communication we have studied the effects of ionomycin on starfish oocytes and zygotes. By use of confocal microscopy, calcium imaging, as well as light and transmission electron microscopy, we have demonstrated that immature oocytes exposed to ionomycin instantly increase intracellular Ca(2+) levels and undergo structural changes in the cortex. Surprisingly, when microinjected into the cells, ionomycin produced no Ca(2+) increase. The ionomycin-induced Ca(2+) rise was followed by fast alteration of the actin cytoskeleton displaying conspicuous depolymerization at the oocyte surface and in microvilli with concomitant polymerization in the cytoplasm. In addition, cortical granules were disrupted or fused with white vesicles few minutes after the addition of ionomycin. These structural changes prevented cortical maturation of the eggs despite the normal progression of nuclear envelope breakdown. At fertilization, the ionomycin-pretreated eggs displayed reduced Ca(2+) response, no elevation of the fertilization envelope, and the lack of orderly centripetal translocation of actin fibers. These alterations led to difficulties in cell cleavage in the monospermic zygotes and eventually to a higher rate of abnormal development. In conclusion, ionomycin has various deleterious impacts on egg activation and the subsequent embryonic development in starfish. Although direct comparison is difficult to make between our findings and the use of the ionophore in the in vitro fertilization clinics, our results call for more defining investigations on the issue of a potential risk in artificial egg activation.
Laboratory of Cellular and Developmental Biology, Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy.
Filip
F
Vasilev
Jong T
JT
Chun
Giovanni
G
Gragnaniello
Ezio
E
Garante
Luigia
L
Santella
D000199:Actins / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects; D005089:Exocytosis / Q000187:drug effects; D005260:Female; D005306:Fertilization / Q000187:drug effects; D042541:Intracellular Space / Q000378:metabolism; D015759:Ionomycin / Q000008:administration & dosage / Q000494:pharmacology*; D008845:Microinjections; D008871:Microvilli / Q000187:drug effects / Q000648:ultrastructure; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010063:Ovum / Q000187:drug effects* / Q000378:metabolism*; D013215:Starfish / Q000196:embryology* / Q000378:metabolism*
D000199:Actins; D015759:Ionomycin; D002118:Calcium