Investigation of the hyper-reflective inner/outer segment band in optical coherence tomography of living frog retina.
This study is to test anatomic correlates, including connecting cilium (CC) and inner segment (IS) ellipsoid, to the hyper-reflective band visualized by optical coherence tomography (OCT) and commonly attributed to the photoreceptor inner/outer segment (IS/OS) junction. A line-scan OCT (LS-OCT) was constructed to achieve sub-cellular resolution (lateral: ≈ 2 μm; axial: ≈ 4 μm) of excised living frog retinas. An electro-optic phase modulator was employed for rapid and vibration-free phase modulation. Comparison of normalized distance measurements between LS-OCT images and histological images revealed that the dominant source of the signal reported as the IS/OS OCT band actually originates from the IS.
Rong-Wen
RW
Lu
Christine A
CA
Curcio
Youwen
Y
Zhang
Qiu-Xiang
QX
Zhang
Steven J
SJ
Pittler
Dusanka
D
Deretic
Xin-Cheng
XC
Yao
D000465:Algorithms; D000818:Animals; D004594:Electrophysiology / Q000379:methods; D004867:Equipment Design; D008027:Light; D055095:Optics and Photonics; D020419:Photoreceptor Cells, Vertebrate / Q000473:pathology; D011898:Ranidae; D015203:Reproducibility of Results; D012160:Retina / Q000033:anatomy & histology / Q000502:physiology*; D055214:Retinal Photoreceptor Cell Outer Segment / Q000502:physiology*; D012815:Signal Processing, Computer-Assisted; D041623:Tomography, Optical Coherence / Q000379:methods*