Toxicity evaluation of CdTe quantum dots with different size on Escherichia coli.
Quantum dots (QDs) have a great potential for applications in nanomedicine. However, a few studies showed that they also exhibited toxicity. We used Escherichia coli (E. coli) as the model to study the effect of CdTe QDs on the cell growth by microcalorimetric technique, optical density (OD(600)) and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectra. Three size aqueous-compatible CdTe QDs with maximum emission of 543 nm (green-emitting QDs, GQDs), 579 nm (yellow-emitting QDs, YQDs) and 647 nm (red-emitting QDs, RQDs) were tested. The growth rate constants (k) and half-inhibiting concentration (IC(50)) were calculated from the microcalorimetric data. The results indicated that CdTe QDs exhibited a dose-dependent inhibitory effect on cell growth. The order of toxicity is GQDs>YQDs>RQDs. The smaller the particle size of QDs is, the more toxicity it is. ATR-FTIR spectra indicated that the outer membrane of the cell was changed or damaged by the QDs, which may induce QDs and harmful by-products to enter into the cells. These could be one of the reasons that CdTe QDs have cytotoxic effects on E. coli.
Department of Chemistry, School of Science, Wuhan University of Technology, Wuhan 430070, PR China. fangtingting502@sina.com
Ting-Ting
TT
Fang
Xi
X
Li
Qi-Sui
QS
Wang
Zhi-Jun
ZJ
Zhang
Peng
P
Liu
Chao-Can
CC
Zhang
D000826:Animal Testing Alternatives; D019187:Cadmium Compounds / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D020128:Inhibitory Concentration 50; D010316:Particle Size; D045663:Quantum Dots; D013691:Tellurium / Q000633:toxicity*; D018675:Toxicity Tests
D019187:Cadmium Compounds; D013691:Tellurium; C028337:cadmium telluride