Complete genome comparison of duck hepatitis virus type 1 parental and attenuated strains.
Two complete duck hepatitis virus type 1 (DHV-1) genomes, strain SY5 and its chicken embryos passage descendent vaccine strain ZJ-A, were compared and analyzed in order to identify possible sites of attenuation. Of the 205 nucleotide changes, 22 resulted in sense mutations, 174 produced nonsense mutations. Besides, there are 7 consistent nucleotides substitutions in 5'UTR and 2 in 3'UTR. Three of these 22 sense mutations resided in VP0, 6 exists in VP1, one exists in VP3, 3 exists in 2A2, 3 exists in 2C, one was detected in 3B and 5 was in 3D. These results suggested that VP0, VP1, 3D, and 5'/3'UTR may contribute to the attenuation of DHV-1 in chicken/duck/embryos. The results provide a genetic basis for future manipulation of a DHV-1 infectious clone.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
Yong
Y
Wang
Chuanfeng
C
Li
Zongyan
Z
Chen
Binrui
B
Xu
Gang
G
Li
Guangqing
G
Liu
D000818:Animals; D002642:Chick Embryo; D016000:Cluster Analysis; D018389:Codon, Nonsense; D004252:DNA Mutational Analysis; D016679:Genome, Viral; D004371:Hepatitis Virus, Duck / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D020125:Mutation, Missense; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*
D018389:Codon, Nonsense; D012367:RNA, Viral; D014764:Viral Proteins; D037521:Virulence Factors