Differential in vivo gene expression of major Leptospira proteins in resistant or susceptible animal models.
Transcripts of Leptospira 16S rRNA, FlaB, LigB, LipL21, LipL32, LipL36, LipL41, and OmpL37 were quantified in the blood of susceptible (hamsters) and resistant (mice) animal models of leptospirosis. We first validated adequate reference genes and then evaluated expression patterns in vivo compared to in vitro cultures. LipL32 expression was downregulated in vivo and differentially regulated in resistant and susceptible animals. FlaB expression was also repressed in mice but not in hamsters. In contrast, LigB and OmpL37 were upregulated in vivo. Thus, we demonstrated that a virulent strain of Leptospira differentially adapts its gene expression in the blood of infected animals.
Institut Pasteur de Nouvelle-Calédonie, Institut Pasteur International Network, Bacterial Research Laboratory, Noumea, New Caledonia.
Mariko
M
Matsui
Marie-Estelle
ME
Soupé
Jérôme
J
Becam
Cyrille
C
Goarant
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001769:Blood / Q000382:microbiology; D006224:Cricetinae; D004195:Disease Models, Animal; D060467:Disease Resistance; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D054884:Host-Pathogen Interactions; D007919:Leptospira / Q000235:genetics*; D007922:Leptospirosis / Q000382:microbiology*; D051379:Mice; D012336:RNA, Ribosomal, 16S / Q000096:biosynthesis; D014774:Virulence
D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S