Metabolic enzyme diversity in different human thyroid cell lines and their sensitivity to gravitational forces.
Many cancer cells show unique protein expression patterns. We used proteome technology including MS, free flow isoelectric focusing and Western blotting to determine current concentrations of metabolic enzymes in healthy and malignant human thyroid cells. Three different types of human thyroid cells were investigated after they had been cultured under equal conditions. MS revealed high quantities of glycolytic enzymes and moderate quantities of citric acid cycle enzymes in malignant FTC-133 cells with abnormal LDH B-chains, high quantities of glycolytic enzymes and marginal quantities of citric acid cycle enzymes in normal HTU-5 cells, and low quantities of glycolytic enzymes and marginal quantities of citrate cycle enzymes in malignant CGTH-W1 cells with abnormal LDH A-chains. When an alteration of gene expression activity was challenged physically by removing gravity forces, the concentrations of various glycolytic enzymes were changed in normal and malignant thyroid cells. However, the changes varied among the different cell types. Different cellular alignment of the enzymes could be one reason for the cell type-specific behavior as demonstrated by histological analysis of alpha-enolase.
Plastic, Aesthetic and Hand Surgery, Otte-von Guericke University, Magdeburg, Germany.
Jessica
J
Pietsch
Albert
A
Sickmann
Gerhard
G
Weber
Johann
J
Bauer
Marcel
M
Egli
Robert
R
Wildgruber
Manfred
M
Infanger
Daniela
D
Grimm
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006019:Glycolysis; D006112:Gravitation; D006801:Humans; D051379:Mice; D010100:Oxygen / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D021381:Protein Transport; D013961:Thyroid Gland / Q000166:cytology* / Q000201:enzymology*; D013997:Time Factors; D014305:Triose-Phosphate Isomerase / Q000378:metabolism
D003470:Culture Media; D004798:Enzymes; D010751:Phosphopyruvate Hydratase; D014305:Triose-Phosphate Isomerase; D010100:Oxygen