Electrochromatographic enantioseparation of amino acids using polybutylmethacrylate-based chiral monolithic column by capillary electrochromatography.
This article describes the development of a polybutylmethacrylate-based monolithic capillary column as a chiral stationary phase. The chiral monolithic column was prepared by polymerization of butyl methacrylate (BMA), ethylene dimethacrylate (EDMA), and N-methacryloyl-L-glutamic acid (MAGA) in the presence of porogens. The porogen mixture included N,N-dimethyl formamide and phosphate buffer. MAGA was used as a chiral selector. The effect of MAGA content was investigated on electrochromatographic enantioseparation of D,L-histidine, D,L-tyrosine, D,L-phenyl alanine, and D,L-glutamic acid. The effect of acetonitrile (ACN) content in mobile phase on electro-osmotic flow was also investigated. It was demonstrated that the poly(BMA-EDMA-MAGA) monolithic chiral column can be used for the electrochromatographic enantioseparation of amino acids by capillary electrochromatography (CEC). The mobile phase was ACN/10 mM phosphate buffer (45:55%) adjusted to pH 2.7. It was observed that L-enantiomers of the amino acids migrated before D-enantiomers. The separation mechanism of electrochromatographic enantioseparation of amino acids in CEC is discussed.
Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey.
Cemil
C
Aydogan
Adil
A
Denizli
D000596:Amino Acids / Q000737:chemistry* / Q000302:isolation & purification*; D053801:Capillary Electrochromatography / Q000379:methods*; D018698:Glutamic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry*; D058105:Polymerization; D013237:Stereoisomerism
D000596:Amino Acids; D008689:Methacrylates; C115674:N-methacryloyl-glutamic acid; C116863:poly(butyl methacrylate); D018698:Glutamic Acid; C004919:ethylene dimethacrylate