Biosynthesis, extraction, purification, and analysis of trisporoid sexual communication compounds from mated cultures of Blakeslea trispora.
The zygomycete Blakeslea trispora produces high amounts of the general zygomycete β-carotene-derived sexual signal compounds, the trisporoids. These can be isolated from the culture medium and purified by extraction with organic solvents followed by thin layer chromatography. Concentration is determined spectrophotometrically using specific extinction coefficients established for some members of this compound family. The effect of the extraction and activity of the isolated compounds is best tested physiologically, exploiting the ability of trisporoids to induce the formation of sexually committed hyphae, the zygophores, in other zygomycete species. Methods for B. trispora culture, trisporoid extraction, and further analyses of trisporoids are described in this chapter.
General Microbiology and Microbial Genetics, Institute of Microbiology, Friedrich-Schiller-University Jena, Jena, Germany. Christine.Schimek@uni-jena.de
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D005591:Chemical Fractionation / Q000379:methods*; D002855:Chromatography, Thin Layer; D046508:Culture Techniques; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D025301:Hyphae / Q000254:growth & development / Q000378:metabolism; D009089:Mucor / Q000187:drug effects; D009090:Mucorales / Q000254:growth & development* / Q000378:metabolism*; D010675:Pheromones / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D019207:beta Carotene / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000302:isolation & purification* / Q000494:pharmacology
D005231:Fatty Acids, Unsaturated; D010675:Pheromones; D019207:beta Carotene; C004574:trisporic acid