Identification of serum biomarkers for lung cancer using protein mass             spectrometry.
Prior to pathological changes becoming apparent in any disease, the component             and amount of intracellular proteins may undergo alteration. Thus, monitoring             of proteins may be used to screen indicators in order to identify prognostic markers.             The aim of this study was to investigate the feasibility of identification of             serum biomarkers for lung cancer using protein mass spectrometry. Surface-enhanced             laser desorption/ionization (SELDI) and weak cation exchange 2 (WCX2) protein             chip array were employed for protein profiling of the sera of 17 healthy rabbits             and 23 cancer‑bearing rabbits, of which 15 developed cancer in the lung (cancer             group) and 8 developed lung cancer in the follow-up period (pre-cancer group).             Data were obtained using a PBSII-C protein chip reader and analyzed using Biomarker             Wizard and Proteinchip 3.1 software. Compared with the healthy rabbits, a total             of 5 biomarkers were identified to be differentially expressed among 32 proteins             screened from the sera in the cancer group and the pre-cancer group (P<0.05):             3 of the 5 biomarkers were upregulated and 2 were downregulated. Protein mass             spectro-metry can be used to identify specific molecules closely correlated with             the progression of lung cancer and, thus, this method may become an effective             tool for the early diagnosis or prediction of lung cancer.
Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing 100853, PR China.
Xin
X
Li
Weixing
W
Liu
Baowei
B
Dong
Lin
L
Sheng
He
H
Ren
Zhiyu
Z
Han
Tong
T
Lu
Ping
P
Liang
D000818:Animals; D015415:Biomarkers / Q000097:blood*; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D040081:Protein Array Analysis; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014057:Tomography, X-Ray Computed
D015415:Biomarkers