A map of nucleosome positions in yeast at base-pair resolution.
The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.
Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, USA.
Kristin
K
Brogaard
Liqun
L
Xi
Ji-Ping
JP
Wang
Jonathan
J
Widom
D020029:Base Pairing; D003545:Cysteine / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D016681:Genome, Fungal / Q000235:genetics; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D009707:Nucleosomes / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000378:metabolism
D006657:Histones; D009707:Nucleosomes; D014157:Transcription Factors; D017665:Hydroxyl Radical; D012321:DNA-Directed RNA Polymerases; D003545:Cysteine