Amino acid concentrations in the hamster central auditory system and long-term effects of intense tone exposure.
Exposure to intense sounds often leads to loss of hearing of environmental sounds and hearing of a monotonous tonal sound not actually present, a condition known as tinnitus. Chronic physiological effects of exposure to intense tones have been reported for animals and should be accompanied by chemical changes present at long times after the intense sound exposure. By using a microdissection mapping procedure combined with high-performance liquid chromatography (HPLC), we have measured concentrations of nine amino acids, including those used as neurotransmitters, in the cochlear nucleus, inferior colliculus, medial geniculate, and auditory cortex of hamsters 5 months after exposure to an intense tone, compared with control hamsters of the same age. No very large differences in amino acid concentrations were found between exposed and control hamsters. However, increases of glutamate and γ-aminobutyrate (GABA) in some parts of the inferior colliculus of exposed hamsters were statistically significant. The most consistent differences between exposed and control hamsters were higher aspartate and lower taurine concentrations in virtually all regions of exposed hamsters, which reached statistical significance in many cases. Although these amino acids are not considered likely neurotransmitters, they indirectly have roles in excitatory and inhibitory neurotransmission, respectively. Thus, there is evidence for small, widespread, long-term increases in excitatory transmission and decreases in inhibitory transmission after a level of acoustic trauma previously shown to produce hearing loss and tinnitus.
Department of Neurology, University of Toledo College of Medicine, Toledo, Ohio 43614, USA. donald.godfrey@utoledo.edu
Donald A
DA
Godfrey
James A
JA
Kaltenbach
Kejian
K
Chen
Omer
O
Ilyas
Xiaochen
X
Liu
Frank
F
Licari
Justin
J
Sacks
Darwin
D
McKnight
D000161:Acoustic Stimulation / Q000379:methods; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D006317:Hearing Loss, Noise-Induced / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D042282:Microdissection; D013995:Time
D000596:Amino Acids