A novel marker for the species-specific detection and quantitation of Shigella sonnei by targeting a methylase gene.
Shigella sonnei is a causal agent of fever, nausea, stomach cramps, vomiting, and diarrheal disease. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection of S. sonnei using a primer pair based on the methylase gene for the amplification of a 325 bp DNA fragment. The qPCR primer set for the accurate diagnosis of Shigella sonnei was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.
National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea.
Min Seok
MS
Cho
Tae-Young
TY
Ahn
Kiseong
K
Joh
Oh-Sang
OS
Kwon
Won-Hwa
WH
Jheong
Dong Suk
DS
Park
D001431:Bacteriological Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012764:Shigella sonnei / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*
D017931:DNA Primers; D008780:Methyltransferases