[Study on the bioactive constituents of Piper wallichii].
OBJECTIVE
To investigate the bioactive constituents in the stem of Piper wallichii.


METHODS
Compounds were separated by column chromatography of silica gel, ODS-A and Sephadex LH-20. Their structures were elucidated based on spectral analysis. DPPH scavenging activity and AchE inhibitory activity were tested.


RESULTS
10 compounds were isolated and their structures were identified as 3,4-methylenedioxy-benzoic acid (1), vanillic acid (2), benzoic acid (3), N-p-coumaroyltyramine (4), futoenone (5), futoquinol (6), isofutoquinol A (7), 4-hydroxy-3,5-dimethoxy-benzoic acid (8), futoamide (9), dihydropiperlonguminine (10).


CONCLUSIONS
Compounds 1-6 are isolated from P. wallichii for the first time. Vanillic acid (2) and 4-hydroxy-3,5-dimethoxy-benzoic acid (8) show scavenging activity against DPPH radical with ED50 at 224.33 microg/mL and 11.44 microg/mL, respectively. No compound shows inhibition activity against AchE.
School of Pharmacy, Shandong University, Jinan 250012, China.
Guo-Wei
GW
Zhao
Wen
W
Xia
Ping
P
Chen
En-Ji
EJ
Han
Lan
L
Xiang
D001565:Benzoates / Q000737:chemistry* / Q000302:isolation & purification; D019817:Benzoic Acid / Q000737:chemistry / Q000302:isolation & purification; D001713:Biphenyl Compounds / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D003373:Coumaric Acids / Q000737:chemistry / Q000302:isolation & purification; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D062385:Hydroxybenzoates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D010851:Picrates / Q000378:metabolism; D031701:Piper / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D014641:Vanillic Acid / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
C413324:3,5-dimethoxy-4-hydroxybenzoic acid; D001565:Benzoates; D001713:Biphenyl Compounds; D002800:Cholinesterase Inhibitors; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D062385:Hydroxybenzoates; D017705:Lignans; D010851:Picrates; C549141:futoquinol; D019817:Benzoic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D014641:Vanillic Acid; C005455:piperonylic acid