Differentiation of neotropical fish species with statistical analysis of fourier transform infrared photoacoustic spectroscopy data.
Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was applied to nineteen fish species in Brazil's Upper Paraná River basin to identify differences in the structural composition of their scales. To differentiate the species, a canonical discriminant analysis was  used to indicate the most important absorption peaks in the mid-infrared region. Significant differences were found in the chemical composition of scales among the studied fish species, with Wilk's lambda = 5.2 × 10(-6), F((13,18,394)) = 37.57, and P  < 0.001, indicating that O-CH(2) wag at 1396 cm(-1) can be used as a biomarker of this species group. The species could be categorized into four groups according to phylogenetic similarity, suggesting that the O-CH(2) 1396 cm(-1)  absorbance is related to the biological traits of each species. This procedure can also be used to complement evolutionary studies.
Grupo de Espectroscopia Óptica e Fototérmica, Programa de Pós-Graduação em Recursos Naturais, Universidade Estadual de Mato Grosso do Sul, Dourados, MS, Brazil.
Francylaine S
FS
Almeida
Sandro M
SM
Lima
Luis H C
LH
Andrade
Yzel R
YR
Súarez
D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D001938:Brazil; D016002:Discriminant Analysis; D005399:Fishes / Q000145:classification* / Q000235:genetics; D061088:Photoacoustic Techniques / Q000379:methods*; D010802:Phylogeny; D012867:Skin / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D015415:Biomarkers