CD5-positive B cell subsets in secondary progressive multiple sclerosis.
Previous studies have demonstrated that CD5(+) B cells produce more interleukin (IL)-10 than CD5(-) B cells and that CD5(+) B cells confer significant protection against experimental autoimmune encephalomyelitis (EAE). The objective of the present study was to determine whether CD5-positive B cell populations are associated with secondary progressive multiple sclerosis (SPMS) and to explore which subsets on CD5(+) B cells are associated with SPMS. A total of 26 patients with SPMS, of whom 11 were treated with IFNβ (IFN-SPMS) and 15 were not treated (non-IFN-SPMS), and 19 healthy control (HC) subjects were included in the study. Expression levels of CD11a, CD23, CD25, CD38, CD49d, CD80, CD86, CD138, CCR5, and CXCR5 on CD5(+) B cells in blood samples were examined by flow cytometry. The percentage of CD5(+) B cells in the SPMS group was significantly lower than in the HC group. Within the subsets of CD5(+) B cells, the expression of CD11a in the non-IFN-SPMS group was significantly decreased compared to the HC subjects. Patients with SPMS showed lower CCR5, CD25, and CD138 positivity on CD5(+) B cells than HC subjects. Our results indicate that CD5(+) B cell subsets might be associated with pathogenesis of SPMS.
Department of Clinical Research, Hokkaido Medical Center, Yamanote 5-jo 7-chome, Sapporo 063-0005, Japan. niino@hok-mc.hosp.go.jp
Masaaki
M
Niino
Toshiyuki
T
Fukazawa
Naoya
N
Minami
Itaru
I
Amino
Jun
J
Tashiro
Naoto
N
Fujiki
Shizuki
S
Doi
Seiji
S
Kikuchi
D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D018956:CD5 Antigens / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D020528:Multiple Sclerosis, Chronic Progressive / Q000276:immunology* / Q000473:pathology*
D015415:Biomarkers; D018956:CD5 Antigens