Angelicae Gigantis Radix regulates mast cell-mediated allergic inflammation in vivo and in vitro.
Angelicae Gigantis (AG) Radix, commonly used medicinal food, has been reported as a promising candidate for inflammatory diseases. However, the anti-allergic effects of AG and its molecular mechanisms have yet to be clarified. The present study investigated the anti-allergy effects of ethanol extracts of AG on mast cell-mediated allergic inflammation in vivo and in vitro. The finding of this study demonstrated that AG reduced anti-dinitrophenyl IgE antibody-induced passive cutaneous anaphylaxis, compound 48/80-induced histamine release, 2,4-dinitrofluoro benzene-induced contact hypersensitivity. In addition, AG inhibited the production of interleukin (IL)-6, IL-8, and TNF-α, as well as the activation of Jun N-terminal kinase and nuclear factor-κB in phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated human mast cells. In conclusion, our results provide a novel insight into the pharmacological actions of AG as a potential candidate for use in allergic inflammatory diseases.
Department of Anatomy, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Youngjoo
Y
Sohn
Hyun-Sam
HS
Lee
Hi-Joon
HJ
Park
Hyangsook
H
Lee
Hyejung
H
Lee
Hyeon
H
Choi
Chang-Hyun
CH
Jeong
Youngmin
Y
Bu
Hyuk-Sang
HS
Jung
D029969:Angelica / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006636:Histamine Release / Q000187:drug effects; D006967:Hypersensitivity / Q000276:immunology / Q000517:prevention & control*; D066298:In Vitro Techniques; D007249:Inflammation / Q000276:immunology / Q000517:prevention & control*; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D010936:Plant Extracts