New MLSB resistance gene erm(43) in Staphylococcus lentus.
The search for a specific rRNA methylase motif led to the identification of the new macrolide, lincosamide, and streptogramin B resistance gene erm(43) in Staphylococcus lentus. An inducible resistance phenotype was demonstrated by cloning and expressing erm(43) and its regulatory region in Staphylococcus aureus. The erm(43) gene was detected in two different DNA fragments, of 6,230 bp and 1,559 bp, that were each integrated at the same location in the chromosome in several S. lentus isolates of human, dog, and chicken origin.
Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Sybille
S
Schwendener
Vincent
V
Perreten
D018829:Adhesins, Bacterial / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002645:Chickens; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004285:Dogs; D024881:Drug Resistance, Bacterial; D006801:Humans; D055231:Lincosamides / Q000737:chemistry / Q000494:pharmacology; D018942:Macrolides / Q000737:chemistry / Q000494:pharmacology; D008780:Methyltransferases / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics; D025381:Streptogramin B / Q000737:chemistry / Q000494:pharmacology
D018829:Adhesins, Bacterial; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D055231:Lincosamides; D018942:Macrolides; D011994:Recombinant Proteins; D025381:Streptogramin B; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase