In vivo genotoxicity study of single-wall carbon nanotubes using comet assay following intratracheal instillation in rats.
The genotoxicity of single-wall carbon nanotubes (SWCNTs) was evaluated in vivo using the comet assay after intratracheal instillation in rats. The SWCNTs were instilled at a dosage of 0.2 or 1.0mg/kg body weight (single instillation group) and 0.04 or 0.2mg/kg body weight once a week for 5weeks (repeated instillation group). As a negative control, 1% Tween 80 was instilled in a similar manner. As a positive control, ethyl methanesulfonate (EMS) at 500mg/kg was administered once orally 3h prior to dissection. Histopathologically, inflammation in the lung was observed for all the SWCNTs in both single and repeated groups. In the comet assay, there was no increase in% tail DNA in any of the SWCNT-treated groups. In the EMS-treated groups, there was a significant increase in% tail DNA compared with the negative control group. The present study indicated that a single intratracheal instillation of SWCNTs (1.0mg/kg) or repeated intratracheal instillation (0.2mg/kg) once a week for five weeks induced a clear inflammatory response (hemorrhage in the alveolus, infiltration of alveolar macrophages and neutrophiles), but no DNA damage, in the lungs in rats. Under the conditions of the test, SWCNTs were not genotoxic in the comet assay following intratracheal instillation in rats.
Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan. masato.naya@aist.go.jp
Masato
M
Naya
Norihiro
N
Kobayashi
Shigehisa
S
Endoh
Junko
J
Maru
Kazumasa
K
Honda
Makoto
M
Ema
Jin
J
Tanaka
Masahito
M
Fukumuro
Kazushige
K
Hasegawa
Madoka
M
Nakajima
Makoto
M
Hayashi
Junko
J
Nakanishi
D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D019570:Inhalation Exposure / Q000009:adverse effects; D007442:Intubation, Intratracheal / Q000009:adverse effects; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D009153:Mutagens / Q000008:administration & dosage / Q000145:classification / Q000633:toxicity*; D037742:Nanotubes, Carbon / Q000145:classification / Q000633:toxicity*; D011014:Pneumonia / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains
D009153:Mutagens; D037742:Nanotubes, Carbon