Molecular drivers of base flipping during sequence-specific DNA methylation.
One step at a time: Substrates containing nucleotide analogues lacking sequence-specific contacts to the C5 methyltransferase M.HhaI were used to probe the role of individual interactions in effecting conformational transitions during base flipping. A segregation of duties, that is, specific recognition and chemomechanical force for base flipping and active site assembly, within the enzyme is confirmed.
Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA 93106-9510, USA.
Douglas M
DM
Matje
Norbert O
NO
Reich
D001483:Base Sequence; D019175:DNA Methylation; D015257:DNA-Cytosine Methylases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006860:Hydrogen Bonding; D008958:Models, Molecular
D015257:DNA-Cytosine Methylases