Enhanced photoinactivation of Staphylococcus aureus with nanocomposites containing plasmonic particles and hematoporphyrin.
We fabricated composite nanoparticles consisting of a plasmonic core (gold nanorods or gold-silver nanocages) and a hematoporphyrin-doped silica shell. The dual photodynamic and photothermal activities of such nanoparticles against Staphylococcus aureus 209 P were studied and compared with the activities of reference solutions (hematoporphyrin or silica-coated plasmonic nanoparticles). Bacteria were incubated with nanocomposites or with the reference solutions for 15 min, which was followed by CW light irradiation with a few exposures of 5 to 30 min. To stimulate the photodynamic and photothermal activities of the nanocomposites, we used LEDs (405 and 625 nm) and a NIR laser (808 nm), respectively. We observed enhanced inactivation of S. aureus 209 P by nanocomposites in comparison with the reference solutions. By using fluorescence microscopy and spectroscopy, we explain the enhanced antimicrobial effect of hematoporphyrin-doped nanocomposites by their selective accumulation in the vicinity of the bacteria.
Institute of Biochemistry and Physiology of Plants and Micro-organisms, RAS, 13 Prospekt Entuziastov, Saratov 410049, Russia. khlebtsov@ibppm.sgu.ru
Boris N
BN
Khlebtsov
Elena S
ES
Tuchina
Vitaly A
VA
Khanadeev
Elizaveta V
EV
Panfilova
Pavel O
PO
Petrov
Valery V
VV
Tuchin
Nikolai G
NG
Khlebtsov
D006046:Gold / Q000737:chemistry; D006415:Hematoporphyrins / Q000737:chemistry*; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry; D050296:Microbial Viability / Q000528:radiation effects*; D053761:Nanocomposites / Q000737:chemistry*; D055620:Optical Phenomena; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D013696:Temperature
D006415:Hematoporphyrins; D017319:Photosensitizing Agents; D012834:Silver; D006046:Gold; D012822:Silicon Dioxide