title: A novel strategy for phosphopeptide enrichment using lanthanide phosphate co-precipitation.
abstract: Reversible phosphorylation of proteins is a common theme in the regulation of important cellular functions such as growth, metabolism, and differentiation. The comprehensive understanding of biological processes requires the characterization of protein phosphorylation at the molecular level. Although, the number of cellular phosphoproteins is relatively high, the phosphorylated residues themselves are generally of low abundance due to the sub-stoichiometric nature. However, low abundance of phosphopeptides and low degree of phosphorylation typically necessitates isolation and concentration of phosphopeptides prior to mass spectrometric analysis. In this study, we used trivalent lanthanide ions (LaCl(3), CeCl(3), EuCl(3), TbCl(3), HoCl(3), ErCl(3), and TmCl(3)) for phosphopeptide enrichment and cleaning-up. Due to their low solubility product, lanthanide ions form stable complexes with the phosphate groups of phosphopeptides and precipitate out of solution. In a further step, non-phosphorylated compounds can easily be removed by simple centrifugation and washing before mass spectrometric analysis using Matrix-assisted laser desorption/ionisation-time of flight. The precipitation method was applied for the isolation of phosphopeptides from standard proteins such as ovalbumin, α-casein, and β-casein. High enrichment of phosphopeptides could also be achieved for real samples such as fresh milk and egg white. The technology presented here represents an excellent and highly selective tool for phosphopeptide recovery; it is easily applicable and shows several advantages as compared with standard approaches such as TiO(2) or IMAC.
authors: Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University of Innsbruck, Innsbruck, Austria.
authors: Munazza Raza
authors: MR
authors: Mirza
authors: Matthias
authors: M
authors: Rainer
authors: Yüksel
authors: Y
authors: Güzel
authors: Iqbal M
authors: IM
authors: Choudhary
authors: Günther K
authors: GK
authors: Bonn
mesh_terms: D000595:Amino Acid Sequence; D002364:Caseins / Q000737:chemistry; D002498:Centrifugation; D011232:Chemical Precipitation; D006801:Humans; D007811:Lanthanum / Q000737:chemistry*; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry*; D010748:Phosphopeptides / Q000302:isolation & purification*; D010766:Phosphorylation; D012996:Solutions; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013499:Surface Properties
chemical_list: D002364:Caseins; D010710:Phosphates; D010748:Phosphopeptides; D012996:Solutions; C551148:lanthanide phosphate; D007811:Lanthanum; D010047:Ovalbumin