title: Renal expression and function of oat1 and oat3 in rats with vascular calcification.
abstract: OBJECTIVE
  Calcium overload in vascular smooth muscle is a highly pathogenic event, which progresses with advancing age. Old patients are polymedicated, and several pharmacotherapeutic agents circulate in the plasma as organic anions. The organic anion transporters 1 and 3 (Oat1 and Oat3) are present in renal basolateral membranes, which transport organic anions of pharmacological and physiological interest. This study was designed to evaluate the renal expression and function of Oat1 and Oat3 in rats with vascular calcification.
  
  
  METHODS
  Vascular calcification was induced by administration of a single dose of vitamin D(3) (300,000 UI/ kg b.w., i.m.) to male Wistar rats 10 days before the experiments. Oat1 and Oat3 expression was assessed by immunoblotting, immunohistochemistry and reverse-transcriptase polymerase chain reaction. The renal clearance of p-aminohippurate (PAH, a prototypical organic anion, substrate of Oat1 and Oat3) was measured by conventional clearance techniques.
  
  
  RESULTS
  Oat1 and Oat3 protein levels showed an increase in plasma membranes of renal proximal tubules of treated animals, where both transporters are functional. This could explain the increase observed in the renal clearance of PAH in treated rats.
  
  
  CONCLUSIONS
  These results suggest the relevance of considering the existence of vascular calcification, which is common in ageing, when organic anion drugs are prescribed.
authors: Area Farmacología, Facultad de Ciencias Bioquímicas y Farmacéuticas, CONICET, Universidad Nacional de Rosario, Rosario, Argentina.
authors: Romina
authors: R
authors: Bulacio
authors: María Herminia
authors: MH
authors: Hazelhoff
authors: Adriana Mónica
authors: AM
authors: Torres
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000378:metabolism; D062186:Arterial Pressure; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D002762:Cholecalciferol; D004195:Disease Models, Animal; D007668:Kidney / Q000473:pathology / Q000502:physiology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000503:physiopathology*; D027362:Organic Anion Transport Protein 1 / Q000502:physiology*; D029362:Organic Anion Transporters, Sodium-Independent / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D061205:Vascular Calcification / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D010130:p-Aminohippuric Acid / Q000378:metabolism
chemical_list: D027362:Organic Anion Transport Protein 1; D029362:Organic Anion Transporters, Sodium-Independent; D012333:RNA, Messenger; C421043:Slc22a6 protein, rat; C114338:organic anion transport protein 3; D002762:Cholecalciferol; D002118:Calcium; D010130:p-Aminohippuric Acid