title: In vitro inhibitory effect on pancreatic lipase activity of subfractions from ethanol extracts of fermented Oats (Avena sativa L.) and synergistic effect of three phenolic acids.
abstract: The purpose of the present work is to study the pancreatic lipase inhibitory effects of different subfractions (n-hexane, ethyl acetate (EA), n-butanol, and water) from ethanol extracts of nonfermented and fungi-fermented oats and to delineate the interactions of three primary phenolic acids in the EA subfractions. The EA subfraction showed the highest inhibitory effect on pancreatic lipase activity at 1.5 mg/mL compared to the other subfractions, regardless of whether the oats were fermented. Meanwhile, both of the EA subfractions of two fungi-fermented oats demonstrated more effective inhibitory activity than that of nonfermented oats. A positive correlation between the total phenolics content and inhibitory activity was found. The inhibitory ability of the EA subfraction from nonfermented or fermented oats also displayed a dose-dependent effect. The standards of caffeic, ferulic, and p-coumaric acids, mainly included in EA subfractions of fermented oats, also displayed a dose-dependent inhibitory effect. A synergistic effect of each binary combination of p-coumaric, ferulic, and caffeic acids was observed, especially at 150.0 μg/mL. Those results indicate that fungi-fermented oats have a more effective inhibitory ability on pancreatic lipase and polyphenols may be the most effective component and could be potentially used for dietary therapy of obesity.
authors: College of Food Science & Nutritional Engineering, China Agricultural University, 100083 Beijing, People's Republic of China.
authors: Shengbao
authors: S
authors: Cai
authors: Ou
authors: O
authors: Wang
authors: Mengqian
authors: M
authors: Wang
authors: Jianfeng
authors: J
authors: He
authors: Yong
authors: Y
authors: Wang
authors: Di
authors: D
authors: Zhang
authors: Feng
authors: F
authors: Zhou
authors: Baoping
authors: B
authors: Ji
mesh_terms: D000085:Acetates; D000818:Animals; D018554:Avena / Q000737:chemistry*; D002109:Caffeic Acids / Q000494:pharmacology; D005591:Chemical Fractionation; D003373:Coumaric Acids / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005285:Fermentation; D008049:Lipase / Q000037:antagonists & inhibitors*; D010179:Pancreas / Q000201:enzymology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D011422:Propionates
chemical_list: D000085:Acetates; D002109:Caffeic Acids; D003373:Coumaric Acids; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D011422:Propionates; C007650:ethyl acetate; C004999:ferulic acid; D008049:Lipase; C495469:p-coumaric acid; C040048:caffeic acid