title: MicroRNA-124 is a subventricular zone neuronal fate determinant.
abstract: New neurons are continuously generated from neural stem cells with astrocyte properties, which reside in close proximity to the ventricle in the postnatal and adult brain. In this study we found that microRNA-124 (miR-124) dictates postnatal neurogenesis in the mouse subventricular zone. Using a transgenic reporter mouse we show that miR-124 expression is initiated in the rapid amplifying progenitors and remains expressed in the resulting neurons. When we stably inhibited miR-124 in vivo, neurogenesis was blocked, leading to the appearance of ectopic cells with astrocyte characteristics in the olfactory bulb. Conversely, when we overexpressed miR-124, neural stem cells were not maintained in the subventricular zone and neurogenesis was lost. In summary, our results demonstrate that miR-124 is a neuronal fate determinant in the subventricular zone.
authors: Department of Experimental Medical Science, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, 22184 Lund, Sweden.
authors: Malin
authors: M
authors: Åkerblom
authors: Rohit
authors: R
authors: Sachdeva
authors: Isabelle
authors: I
authors: Barde
authors: Sonia
authors: S
authors: Verp
authors: Bernhard
authors: B
authors: Gentner
authors: Didier
authors: D
authors: Trono
authors: Johan
authors: J
authors: Jakobsson
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002552:Cerebral Ventricles / Q000166:cytology*; D005260:Female; D005822:Genetic Vectors / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D058953:Neural Stem Cells / Q000502:physiology*; D055495:Neurogenesis / Q000235:genetics*; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D014161:Transduction, Genetic
chemical_list: D005904:Glial Fibrillary Acidic Protein; D035683:MicroRNAs; C540079:Mirn124 microRNA, mouse