title: Enantioseparation of chiral acids and bases on a clindamycin phosphate-modified zirconia monolith by capillary electrochromatography.
abstract: Porous zirconia monolith modified with clindamycin phosphate (CLIP-ZM) was used as chiral stationary phase (CSP) to separate a set of six acidic and basic chiral compounds in capillary electrochromatography (CEC). Resolutions and chiral selectivity factors of the chiral compounds were measured in ACN/MeOH mobile phases of varying compositions of MeOH and ammonium acetate as the electrolyte. In contrast to the CE separations where only chiral separations of acidic compounds were achieved enantiomers of both acidic and basic compounds were separated with acidic compounds better resolved than basic ones by CEC on the CLIP-ZM CSP. Best chiral separations of the chiral compounds were obtained with an eluent containing 10mM ammonium acetate and 35 vol.% methanol.
authors: Department of Chemistry, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
authors: Minji
authors: M
authors: Kim
authors: Department of Chemistry, Yeungnam University, Gyeongsan 712-749, Republic of Korea. Electronic address: jhpark@ynu.ac.kr.
authors: Jung Hag
authors: JH
authors: Park
mesh_terms: D000085:Acetates / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D002981:Clindamycin / Q000031:analogs & derivatives* / Q000737:chemistry; D004573:Electrolytes; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000302:isolation & purification*; D013237:Stereoisomerism; D015040:Zirconium / Q000737:chemistry*
chemical_list: D000085:Acetates; D004573:Electrolytes; D004364:Pharmaceutical Preparations; D002981:Clindamycin; D015040:Zirconium; C007084:clindamycin phosphate; C018824:ammonium acetate; C028541:zirconium oxide; D000432:Methanol