title: Estimating energy expenditure in mice using an energy balance technique.
abstract: OBJECTIVE
  To compare, in mice, the accuracy of estimates of energy expenditure (EE) using an energy balance technique (TEEbal: food energy intake and body composition change) vs indirect calorimetry (TEEIC).
  
  
  METHODS
  In 32 male C57BL/6J mice, EE was estimated using an energy balance (caloric intake minus change in body energy stores) method over a 37-day period. EE was also measured in the same animals by indirect calorimetry. These measures were compared.
  
  
  RESULTS
  The two methods were highly correlated (r(2)=0.87: TEEbal=1.07*TEEIC-0.22, P<0.0001). By Bland-Altman analysis, TEEbal estimates were slightly higher (4.6±1.5%; P<0.05) than TEEIC estimates (Bias=0.55 kcal per 24 h).
  
  
  CONCLUSIONS
  TEEbal can be performed in 'home cages' and provides an accurate integrated long-term measurement of EE while minimizing potentially confounding stress that may accompany the use of indirect calorimetry systems. The technique can also be used to assess long-term energy intake.
authors: Division of Molecular Genetics and Naomi Berrie Diabetes Center, Columbia University, New York, NY 10032, USA.
authors: Y
authors: Y
authors: Ravussin
authors: R
authors: R
authors: Gutman
authors: C A
authors: CA
authors: LeDuc
authors: R L
authors: RL
authors: Leibel
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001823:Body Composition / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D002153:Calorimetry, Indirect / Q000379:methods*; D059305:Diet, High-Fat; D004734:Energy Metabolism / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results