title: Approach to develop a standardized TLC-DPPH• test for assessing free radical scavenging properties of selected phenolic compounds.
abstract: TLC-DPPH(•) test belongs to a group of frequently performed assays aimed at detection of compounds with desired activity (effect directed analysis). Despite its popularity a standard procedure has not been elaborated so far causing difficulties in comparing results obtained in different laboratories. Thus the aim of the presented research was an approach to develop a standardized procedure for assessing free radical scavenging properties of plant polyphenols. It was observed that specifically positive adsorbent (silica gel) strengthened the observed result of radical-antioxidant reaction, while polar bonded stationary phase CN-silica weakened it. Based on the observed results it was concluded that the TLC-DPPH(•) assay should be preferably performed on the surface of non-specific adsorbents (e.g.: RP-18) with the use of n-hexane for DPPH(•) dissolution. It is also proposed to document the results every 5 min after staining, as they change in time.
authors: Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4a, 20 093 Lublin, Poland. lukecarpenter@poczta.onet.pl
authors: Łukasz
authors: Ł
authors: Cieśla
authors: Jakub
authors: J
authors: Kryszeń
authors: Anna
authors: A
authors: Stochmal
authors: Wiesław
authors: W
authors: Oleszek
authors: Monika
authors: M
authors: Waksmundzka-Hajnos
mesh_terms: D000327:Adsorption; D001713:Biphenyl Compounds / Q000737:chemistry*; D002138:Calibration; D002855:Chromatography, Thin Layer / Q000379:methods* / Q000592:standards; D016166:Free Radical Scavengers / Q000737:chemistry*; D006586:Hexanes / Q000737:chemistry; D010851:Picrates / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D012015:Reference Standards; D058428:Silica Gel / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006586:Hexanes; D010851:Picrates; D059808:Polyphenols; D012997:Solvents; C026385:n-hexane; D058428:Silica Gel; C004931:1,1-diphenyl-2-picrylhydrazyl