title: [Vital confocal microscopy in evaluation of efficacy of personalized cell therapy in management of early postoperative bullous keratopathy].
abstract: The results of vital confocal microscopy (VCM) of 5 patients with early postoperative bullous keratopathy after cataract phakoemulsification (PE) treated with author's technique of personalized cell therapy (PCT, intracameral injection of activated patient's leukocytes in autologous serum) are presented. In 3 of all presented cases endothelial loss after PE was more than 50%. Before PCT using of VCM was not possible due to corneal edema. After 2-3 PCT sessions corneal clarity recovery was observed and signs of regenerative processes in cornea were captured by VCM: activation of keratocytes (hyperreflective nuclei) and endothelial cells (multinucleated cells), descemet membrane folds smoothening, normalization of epithelium. Results of VCM correlate with biomicroscopic signs of corneal edema resolution, corneal clarity recovery, improvement of visual acuity after PCT.
authors: E A
authors: EA
authors: Kasparova
authors: N V
authors: NV
authors: Borodina
authors: A M
authors: AM
authors: Subbot
mesh_terms: D002386:Cataract / Q000175:diagnosis / Q000503:physiopathology / Q000628:therapy; D017690:Cell Transplantation / Q000379:methods*; D003315:Cornea / Q000473:pathology / Q000503:physiopathology; D015715:Corneal Edema / Q000175:diagnosis / Q000209:etiology / Q000503:physiopathology / Q000628:therapy; D005260:Female; D006801:Humans; D056965:Injections, Intraocular / Q000379:methods; D007962:Leukocytes; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods*; D008875:Middle Aged; D018918:Phacoemulsification / Q000009:adverse effects* / Q000379:methods; D011183:Postoperative Complications; D016896:Treatment Outcome; D014792:Visual Acuity