title: Fluorescence-based phenotypic selection allows forward genetic screens in haploid human cells.
abstract: The isolation of haploid cell lines has recently allowed the power of forward genetic screens to be applied to mammalian cells. The interest in applying this powerful genetic approach to a mammalian system is only tempered by the limited utility of these screens, if confined to lethal phenotypes. Here we expand the scope of these approaches beyond live/dead screens and show that selection for a cell surface phenotype via fluorescence-activated cell sorting can identify the key molecules in an intracellular pathway, in this case MHC class I antigen presentation. Non-lethal haploid genetic screens are widely applicable to identify genes involved in essentially any cellular pathway.
authors: Cambridge Institute for Medical Research, Addenbrooke's Hospital, Cambridge, United Kingdom.
authors: Lidia M
authors: LM
authors: Duncan
authors: Richard T
authors: RT
authors: Timms
authors: Eszter
authors: E
authors: Zavodszky
authors: Florencia
authors: F
authors: Cano
authors: Gordon
authors: G
authors: Dougan
authors: Felix
authors: F
authors: Randow
authors: Paul J
authors: PJ
authors: Lehner
mesh_terms: D005434:Flow Cytometry; D005453:Fluorescence; D005820:Genetic Testing / Q000379:methods*; D006238:Haploidy; D006801:Humans