title: [Analysis of the relationship between the MecA gene and resistance of β-lactam antibiotics].
abstract: OBJECTIVE
  To investigate the mechanisms by which MecA gene expression leads to β-lactam resistance in methicillin-resistant Staphylococcus aureus (MRSA), and to study the resistance mechanism of MRSA at the molecular level.
  
  
  METHODS
  A variety of molecular biological techniques were employed, including screening MRSA using cefoxitin paper disk method, extraction of MRSA mRNA, reverse transcription into cDNA, real-time fluorescence PCR for quantitation of MecA gene expression, and agar dilution method for assessment of minimum inhibitory concentrations in MRSA treated with cefoxitin, oxacillin, vancomycin, or linezolid.
  
  
  RESULTS
  According to the level of resistance of MRSA to cefoxitin, 40 MRSA strains were divided into a low resistance group (n=12), a middle resistance group (n=15), and a high resistance group (n=13). The expression level of the MecA gene in the low resistance group, the middle resistance group, and the high resistance group was 58.87±30.30, 363.37±200.05, and 1257.72±446.63, respectively. MRSA resistance to cefoxitin and oxacillin was 100%; MRSA resistance to vancomycin or linezolid could not be detected. For all 40 MRSA strains the MIC90 for vancomycin was 2.0 μg/mL.
  
  
  CONCLUSIONS
  MecA gene expression levels may correlate with the MRSA level of resistance to cefoxitin within a certain range of concentration.
authors: Department of Nursing, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors: Hui
authors: H
authors: Huang
authors: Jiandang
authors: J
authors: Zhou
authors: Xinmin
authors: X
authors: Nie
authors: Qifeng
authors: Q
authors: Yi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002440:Cefoxitin / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008826:Microbial Sensitivity Tests / Q000379:methods; D010068:Oxacillin / Q000494:pharmacology; D046915:Penicillin-Binding Proteins
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; D002440:Cefoxitin; D010068:Oxacillin