title: Oxidative damage in various tissues of rats exposed to radon.
abstract: Oxidative damage can be induced by many environmental stressors. 8-Hydroxydeoxyguanosine (8-OHdG) has been used as a biomarker of oxidative DNA damage in both in vitro and in vivo studies. In the present study, Wistar rats were exposed to radon gas at a concentration of 100,000Bq/m(3) for 12 h/d for 30, 60, and 120 d, equivalent to cumulative doses of 60, 120, and 240 working level months (WLM), respectively. Changes in levels of 8-OHdG, reactive oxygen species (ROS), and total antioxidant (T-AOC), as well as expressions of some DNA repair enzymes such as 8-oxoguanine DNA glycosylase (OGG1) and MutT homolog 1 (oxidized purine nucleoside triphosphatase, MTH1), were determined in rat urine, peripheral blood lymphocytes, and lung after exposure to radon. The results revealed an increase in 8-OHdG and ROS levels, a decrease in T-AOC levels, and reduced OGG1 and MTH1 expression levels. The elevated amount of 8-OHdG in urine or lymphocytes was positively correlated with the cumulative exposure dose, whereas OGG1 and MHT1 expression levels in lung were inversely correlated with cumulative exposure dose. These findings indicate that oxidative damage induced by radon may be involved in radon-induced carcinogenesis.
authors: Department of Toxicology, School of Public Health, Soochow University, Suzhou, China.
authors: Ji-Hua
authors: JH
authors: Nie
authors: Zhi-Hai
authors: ZH
authors: Chen
authors: Xing
authors: X
authors: Liu
authors: Yan-Wen
authors: YW
authors: Wu
authors: Ji-Xiang
authors: JX
authors: Li
authors: Yi
authors: Y
authors: Cao
authors: Tom K
authors: TK
authors: Hei
authors: Jian
authors: J
authors: Tong
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000396:Air Pollutants, Radioactive / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D045647:DNA Glycosylases / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D008168:Lung / Q000378:metabolism; D008297:Male; D009381:Neoplasms, Radiation-Induced / Q000209:etiology / Q000378:metabolism; D018384:Oxidative Stress / Q000528:radiation effects*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011886:Radon / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000396:Air Pollutants, Radioactive; D000975:Antioxidants; D015415:Biomarkers; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D010744:Phosphoric Monoester Hydrolases; D045647:DNA Glycosylases; C483741:OGG1 protein, rat; C078207:8-oxodGTPase; D045643:DNA Repair Enzymes; D003849:Deoxyguanosine; D011886:Radon