title: Analysis of the expression of heat-shock protein 27 in patients with oral lichen planus.
abstract: OBJECTIVE
  Heat-shock protein 27 (hsp27) has been implicated in several biological events. In this experimental study, we aimed at analysing, for the first time, the expression of hsp27 in the diverse stages of oral lichen planus (OLP) lesions.
  
  
  METHODS
  Thirty-six biopsy specimens of patients with OLP and 10 of healthy patients were selected. OLP specimens were divided into three groups: G1 - moderate or mildly active OLP; G2 - active or moderately active atrophic OLP; G3 - mild or inactive atrophic OLP. Hsp27 expression was analysed by immunohistochemistry (staining intensity and percentage of stained cells), and results of staining were compared between the different groups. Gender, age and anatomical location were also studied.
  
  
  RESULTS
  In the basal layer, an increase of hsp27 expression in both G2 and G3 was observed when compared to G1 and control group. In contrast, a decrease of hsp27 expression in the superficial layer was observed in all groups when compared to control group.
  
  
  CONCLUSIONS
  The increased expression of Hsp27 in the basal layer observed during the OLP evolution and the less staining in the superficial layers in all cases of OLP suggest that hsp27 may have a role in the OLP pathogenesis.
authors: Department of Stomatology III, Faculty of Odontology, Complutense University of Madrid, Madrid, Spain.
authors: V
authors: V
authors: García-García
authors: A
authors: A
authors: Bascones-Martínez
authors: A I
authors: AI
authors: García-Kass
authors: C P
authors: CP
authors: Martinelli-Kläy
authors: R
authors: R
authors: Küffer
authors: E
authors: E
authors: Álvarez-Fernández
authors: T
authors: T
authors: Lombardi
mesh_terms: D000328:Adult; D001284:Atrophy; D001706:Biopsy; D002467:Cell Nucleus / Q000473:pathology; D004396:Coloring Agents; D003593:Cytoplasm / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins; D017676:Lichen Planus, Oral / Q000145:classification / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology; D014059:Tongue / Q000473:pathology
chemical_list: D004396:Coloring Agents; D055551:HSP27 Heat-Shock Proteins; D007633:Keratins