title: The protective and healing effects of Si Shen Wan in trinitrobenzene sulphonic acid-induced colitis.
abstract: BACKGROUND
  Si Shen Wan is a traditional Chinese herbal medicine formula for the treatment of diseases with diarrhea, such as ulcerative colitis, allergic colitis and chronic colitis. To investigate the protective and healing effects of Si Shen Wan in the experimental colitis induced by trinitrobenzene sulphonic acid, and to furture explore its mechanism of action.
  
  
  METHODS
  Rats with colitis treated with Si Shen Wan for 10 days. Colon wet weight, colon organ coefficient, colonic damage score and pathological change after trinitrobenzene sulphonic acid challenge were determined. The levels of MPO, MDA, GSH-PX, SOD and the expression of IL-4 and IL-10 mRNA in the colon were also measured.
  
  
  RESULTS
  After treatment, colon wet weight, colon organ coefficient and colonic damage score were lower than that in the control group (p<0.05). MDA and MPO concentrations in the inflamed colonic tissues were decreased remarkably in the treated groups compared with that in the control group (p<0.05). But SOD level, IL-4 and IL-10 mRNA expression in the inflamed colonic tissues were obviously increased.
  
  
  CONCLUSIONS
  It is a potential path that protective effect of Si Shen Wan on impaired colonic mucosa rats with experimental colitis was accomplished by down-regulating the level of MDA and MPO, and up-regulating the level of SOD and the IL-4 and IL-10 mRNA expression in the colon mucosa.
authors: JiangXi University of Traditional Chinese Medicine, NanChang, 330004 JiangXi Province, China.
authors: Duan-yong
authors: DY
authors: Liu
authors: Yong-mei
authors: YM
authors: Guan
authors: Hai-mei
authors: HM
authors: Zhao
authors: Dong-mei
authors: DM
authors: Yan
authors: Wen-ting
authors: WT
authors: Tong
authors: Pan-ting
authors: PT
authors: Wan
authors: Wei-feng
authors: WF
authors: Zhu
authors: Hong-ning
authors: HN
authors: Liu
authors: Xin-li
authors: XL
authors: Liang
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D012333:RNA, Messenger; D016753:Interleukin-10; D015847:Interleukin-4; D008315:Malondialdehyde; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase