title: SLM-based off-axis Fourier filtering in microscopy with white light illumination.
abstract: In various microscopy applications spatial light modulators (SLMs) are used as programmable Fourier filters to realize different optical contrast enhancement methods. It is often advantageous to use the SLM in off-axis configuration, where the filtered image wave is sent into the first diffraction order of a blazed grating superposed to the phase mask on the SLM. Because of dispersion this approach is, however, typically limited to spectrally narrowband illumination. Here we suggest a method involving a grating for pre-compensation, which allows one to use spectrally broadband (even thermal) light in SLM-based Fourier filtering. The proposed approach is demonstrated by multicolor imaging of amplitude and phase objects, such as a resolution target, onion epidermal cells and human epithelial cheek cells.
authors: Division for Biomedical Physics, Innsbruck Medical University, Müllerstr. 44, A-6020 Innsbruck, Austria. ruth.steiger@i-med.ac.at
authors: Ruth
authors: R
authors: Steiger
authors: Stefan
authors: S
authors: Bernet
authors: Monika
authors: M
authors: Ritsch-Marte
mesh_terms: D002610:Cheek; D004847:Epithelial Cells / Q000166:cytology; D005583:Fourier Analysis; D006801:Humans; D008027:Light; D008029:Lighting / Q000379:methods*; D008853:Microscopy / Q000379:methods; D019697:Onions / Q000166:cytology; D055095:Optics and Photonics / Q000295:instrumentation*; D019441:Plant Epidermis / Q000166:cytology