title: The subcutaneous adipose tissue reservoir of functionally active stem cells is reduced in obese patients.
abstract: It has been demonstrated that the adipose tissue, a highly functional metabolic tissue, is a reservoir of mesenchymal stem cells. The potential use of adipose-derived stem cells (ADSCs) from white adipose tissue (WAT) for organ repair and regeneration has been considered because of their obvious benefits in terms of accessibility and quantity of available sample. However, the functional capability of ADSCs from subjects with different adiposity has not been investigated. It has been our hypothesis that ADSCs from adipose tissue of patients with metabolic syndrome and high adiposity may be functionally impaired. We report that subcutaneous WAT stromal vascular fraction (SVF) from nonobese individuals had a significantly higher number of CD90+ cells than SVF from obese patients. The isolated ADSCs from WAT of obese patients had reduced differentiation potential and were less proangiogenic. Therefore, ADSCs in adipose tissue of obese patients have lower capacity for spontaneous or therapeutic repair than ADSCs from nonobese metabolically normal individuals.
authors: Cardiovascular Research Center (CSIC-ICCC), Hospital de la Santa Creu i Sant Pau, Av. S. Antoni M. Claret 167, 08025 Barcelona, Spain.
authors: Blanca
authors: B
authors: Oñate
authors: Gemma
authors: G
authors: Vilahur
authors: Raquel
authors: R
authors: Ferrer-Lorente
authors: Juan
authors: J
authors: Ybarra
authors: Alberto
authors: A
authors: Díez-Caballero
authors: Carlos
authors: C
authors: Ballesta-López
authors: Fabrizio
authors: F
authors: Moscatiello
authors: Javier
authors: J
authors: Herrero
authors: Lina
authors: L
authors: Badimon
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000328:Adult; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D015687:Cell Hypoxia / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D009765:Obesity / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D050151:Subcutaneous Fat / Q000166:cytology*; D019700:Thrombospondin 1 / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D019700:Thrombospondin 1