title: Intraspecies variation in Trypanosoma cruzi GPI-mucins: biological activities and differential expression of α-galactosyl residues.
abstract: The glycosylphosphatidylinositol (GPI)-anchored mucins of Trypanosoma cruzi trypomastigotes play an important immunomodulatory role during the course of Chagas disease. Here, some biological activities of tGPI-mucins from four T. cruzi isolates, including benznidazole-susceptible (BZS-Y), benznidazole-resistant (BZR-Y), CL, and Colombiana, were evaluated. GPI-mucins were able to differentially trigger the production of interleukin-12 and nitric oxide in BALB/c macrophages and modulate LLC-MK2 cell invasion. The significance of these variations was assessed after analysis of the terminal α-galactosyl residues. Enzymatic treatment with α-galactosidase indicated a differential expression of O-linked α-galactosyl residues among the strains, with higher expression of this sugar in BZS-Y and BZR-Y T. cruzi populations followed by Colombiana and CL. Unweighted pair group method analysis of the carbohydrate anchor profile and biological parameters allowed the clustering of two groups. One group includes Y and CL strains (T. cruzi II and VI), and the other group is represented by Colombiana strain (T. cruzi I).
authors: Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil. rsoares@cpqrr.fiocruz.br
authors: Rodrigo P
authors: RP
authors: Soares
authors: Ana C
authors: AC
authors: Torrecilhas
authors: Rafael R
authors: RR
authors: Assis
authors: Marcele N
authors: MN
authors: Rocha
authors: Felipe A
authors: FA
authors: Moura e Castro
authors: Gustavo F
authors: GF
authors: Freitas
authors: Silvane M
authors: SM
authors: Murta
authors: Sara L
authors: SL
authors: Santos
authors: Alexandre F
authors: AF
authors: Marques
authors: Igor C
authors: IC
authors: Almeida
authors: Alvaro J
authors: AJ
authors: Romanha
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D017261:Glycosylphosphatidylinositols / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism; D009077:Mucins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D013045:Species Specificity; D014349:Trypanosoma cruzi / Q000378:metabolism*
chemical_list: D016207:Cytokines; D017261:Glycosylphosphatidylinositols; D009077:Mucins; D009573:Nitrites