title: MicroRNA expression profiling of mature ovarian teratomas.
abstract: MicroRNAs (miRNAs) are a class of endogenous, small, non-coding RNAs that regulate gene expression by targeting mRNAs and inhibiting expression via translation repression or RNA degradation. Emerging evidence indicates that miRNAs play a crucial role in the pathogenesis of human diseases, including tumor development. We profiled the miRNA expression between mature ovarian teratoma samples and matched normal tissues using miRNA microarrays, followed by validation with quantitative RT-PCR (qRT-PCR). The most highly expressed miRNAs in mature ovarian teratoma tissues were miRNA-520a-5p, miRNA-26b*, miRNA-421, miRNA-492 and miRNA-555, with a 1.3- to 2.6-fold change, whereas the least expressed miRNAs were miRNA-142-3p, let-7a, miRNA-19a, miRNA-34a, miRNA-620, miRNA-934, miRNA-657, miRNA-720, miRNA-22, miRNA-629 and miRNA-214, with a decreased level of 55-87% compared with normal tissues. The findings of the present study are the first to provide an altered miRNA profile for mature ovarian teratomas and differentially expressed miRNAs, which, if validated in future studies, may be essential in the pathogenesis of mature ovarian teratomas.
authors: Department of Endoscopic Diagnostic and Treatment Center, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, P.R. China.
authors: Ye
authors: Y
authors: Ding
authors: Xiao-Yan
authors: XY
authors: Gu
authors: Feng
authors: F
authors: Xu
authors: Xiao-Yan
authors: XY
authors: Shi
authors: DA-Zheng
authors: DZ
authors: Yang
authors: Jian
authors: J
authors: Zhong
authors: Su-Min
authors: SM
authors: Wang