title: Prevalence of methylenetetrahydrofolate reductase C677T polymorphism in eastern Uttar Pradesh.
abstract: OBJECTIVE
  This study was aimed to evaluate the 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677T mutation in eastern Uttar Pradesh population.
  
  
  METHODS
  Polymerase chain reaction (PCR) using specific primers followed by amplicon digestion by Hinf I restriction enzyme was used for MTHFR C677T polymorphism analysis. Total 250 subjects were analyzed.
  
  
  RESULTS
  The CC genotype was found in 192 subjects, followed by CT in 56 subjects and TT in 2 subject. Genotype frequencies of CC, CT and TT were 0.768, 0.224 and 0.008, respectively. The frequency of C allele was found to be 0.88 and that of T allele was 0.12.
  
  
  CONCLUSIONS
  It is evident from the results of the present study that the percentage of homozygous genotype (CC) is highest in the target population.
authors: Department of Biotechnology, Human Molecular Genetics Laboratory, VBS Purvanchal University, Jaunpur, Uttar Pradesh, India.
authors: Vandana
authors: V
authors: Rai
authors: Upendra
authors: U
authors: Yadav
authors: Pradeep
authors: P
authors: Kumar