title: Effects of total flavonoids from Drynaria fortunei on the proliferation             and osteogenic differentiation of rat dental pulp stem cells.
abstract: Dental pulp stem cells (DPSCs) have the potential to form bone, nerve and             fat, and are a candidate for use in regenerative medicine. Previous studies indicated             that total flavonoids from Drynaria fortunei show a stimulative effect on the             proliferation and osteogenic differentiation of osteoblastic MC3T3-E1 cells in vitro.             This study aimed to investigate the effect of total flavonoids from Drynaria fortunei             on the proliferation and osteogenic differentiation of rat DPSCs, and to further             clarify the mechanisms involved. DPSCs were isolated by enzymatic digestion and             identified using the CD44, CD29 and CD34 markers by immunohistochemistry, and             exposed to 0.01, 0.05 and 0.1 g/l total flavonoids from Drynaria fortunei media.             Total flavonoids from Drynaria fortunei promoted the proliferation of DPSCs in             a dose-dependent manner and this effect may depend on the shortening of the G0/G1             phase and promotion of the S phase. Compared with the control group, the levels             of alkaline phosphatase (ALP) and the expression of osteogenic genes increased             with the concentrations of total flavonoids from Drynaria fortunei, and the volume             and number of calcified nodules in the Drynaria groups was bigger compared to             the control group. These results suggest that total flavonoid from Drynaria fortunei             directly stimulates DPSC proliferation and osteogenic differentiation, and may             serve as a new promising candidate drug for dental tissue engineering and bone             regeneration.
authors: Department of Stomatology, General Hospital of the Yangtze River Shipping, and Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430010, PR China.
authors: Xiao-Fei
authors: XF
authors: Huang
authors: Su-Jian
authors: SJ
authors: Yuan
authors: Cheng
authors: C
authors: Yang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D029621:Polypodiaceae / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016192:Resting Phase, Cell Cycle; D016196:S Phase; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D000469:Alkaline Phosphatase