title: Sustained antioxidant activity of quercetin-loaded lipid-core nanocapsules.
abstract: Lipid-core nanocapsules (LNC) are vesicular nanocarriers prepared by solvent displacement. LNC have been previously prepared using medium-chain triglyceride and sorbitan monostearate as liquid and solid lipophilic components dispersed in the core, surrounded by poly(epsilon-caprolactone) (PCL). Our objective was to investigate the antioxidant activity of LNC containing quercetin (QUE), a radical scavenger, prepared with octyl methoxycinnamate and sorbitan monostearate as lipophilic core components and PCL as the polymer wall. We selected Saccharomyces cerevisae cells as the proposed biological model. QUE-LNC presented z-average diameter of 212 nm, pH of 5.51 and zeta potential of -11 mV. Multiple light scattering analysis (TurbiscanLab) showed a photon path length of 172 microm. Furthermore, a validated turbidimetric study determined that the density of particles in suspension was 1.66 x 10(13). DSC analysis showed that the melting temperature of PCL shifted to lower values when in contact with octyl methoxycinnamate indicating a molecular interaction. After 1 h (7 h), the QUE-LNC formulation and QUE solution incubated with H2O2 showed cell survival of 84.4% (87.7%) and 65.6% (7.3%), respectively. After 35 h of incubation, cell survival was 31.7% and 0.9%, respectively. The QUE-LNC showed sustained antioxidant activity and potential as a nanostructured material to formulate final products.
authors: Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, 90610-000 Porto Alegre RS, Brazil.
authors: Valéria
authors: V
authors: Weiss-Angeli
authors: Fernanda S
authors: FS
authors: Poletto
authors: Samuel L
authors: SL
authors: de Marco
authors: Miriam
authors: M
authors: Salvador
authors: Nádya P
authors: NP
authors: da Silveira
authors: Sílvia S
authors: SS
authors: Guterres
authors: Adriana R
authors: AR
authors: Pohlmann
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053769:Nanocapsules; D011794:Quercetin / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000975:Antioxidants; D008055:Lipids; D053769:Nanocapsules; D011794:Quercetin