title: A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction on endothelial cells.
abstract: OBJECTIVE
  To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction () on the endothelial cell line ECV304.
  
  
  METHODS
  ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoction-containing serum (XFZYD-CS) for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide (MTT), fluorescence activating cell sorter (FACS), migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction (RT-PCR) superarray.
  
  
  RESULTS
  At 48 h, XFZYD-CS induced ECV304 significantly improved cell viability, number in S phase, migration, adhesion and tube formation. At 24 h and 72 h, only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed.
  
  
  CONCLUSIONS
  XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.
authors: Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors: Jun
authors: J
authors: Song
authors: Wen-Yuan
authors: WY
authors: Chen
authors: Li-Ya
authors: LY
authors: Wu
authors: Liang-Pu
authors: LP
authors: Zheng
authors: Wei
authors: W
authors: Lin
authors: Dong
authors: D
authors: Gao
authors: Ted J
authors: TJ
authors: Kaptchuk
authors: Ke-Ji
authors: KJ
authors: Chen
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D043925:Angiogenesis Inducing Agents; D004365:Drugs, Chinese Herbal; C513826:Xue-Fu-Zhu-Yu decoction