title: Althaea rosea Cavanil and Plantago major L. suppress neoplastic cell             transformation through the inhibition of epidermal growth factor receptor kinase.
abstract: For thousands of years in Asia, Althaea rosea Cavanil (ARC) and Plantago major L. (PML) have been used as powerful non-toxic therapeutic agents that inhibit inflammation. However, the anticancer mechanisms and molecular targets of ARC and PML are poorly understood, particularly in epidermal growth factor (EGF)-induced neoplastic cell transformation. The aim of this study was to evaluate the chemopreventive effects and mechanisms of the methanol extracts from ARC (MARC) and PML (MPML) in EGF-induced neoplastic cell transformation of JB6 P+ mouse epidermal cells using an MTS assay, anchorage-independent cell transformation assay and western blotting. Our results showed that MARC and MPML significantly suppressed neoplastic cell transformation by inhibiting the kinase activity of the EGF receptor (EGFR). The activation of EGFR by EGF was suppressed by MARC and MPML treatment in EGFR(+/+) cells, but not in EGFR(-/-) cells. In addition, MARC and MPML inhibited EGF-induced cell proliferation in EGFR-expressing murine embryonic fibroblasts (EGFR(+/+)). These results strongly indicate that EGFR targeting by MARC and MPML may be a good strategy for chemopreventive or chemotherapeutic applications.
authors: Department of Oral Pathology, School of Dentistry and Institute                     of Oral Bioscience, Brain Korea 21 project, Chonbuk National University,                     Jeonju 561-756, Republic of Korea.
authors: Eun-Sun
authors: ES
authors: Choi
authors: Sung-Dae
authors: SD
authors: Cho
authors: Ji-Ae
authors: JA
authors: Shin
authors: Ki Han
authors: KH
authors: Kwon
authors: Nam-Pyo
authors: NP
authors: Cho
authors: Jung-Hyun
authors: JH
authors: Shim
mesh_terms: D031583:Althaea / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D000432:Methanol / Q000737:chemistry; D051379:Mice; D010766:Phosphorylation; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010943:Plantago / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D000432:Methanol