title: Low nitrogen-induced expression of cyclophilin in Nicotiana tabacum.
abstract: Leaf morphology and the leaf protein expression profiles of flue-cured tobacco grown in central Henan province of China under low nitrogen (low-N) and normal nitrogen (normal-N) nutrition were examined. The leaf length and width were measured at 50, 60, and 70 days after transplanting. Leaves grown under low-N conditions were shorter and more narrow than those grown under normal-N conditions. The protein expression profiles of tobacco leaves harvested at 70 days after transplanting were analyzed by 2-dimensional electrophoresis, and five differentially expressed proteins including a putative protein were identified. Except for the MCM protein-like protein, the other three differentially expressed proteins of cyclophilin-like protein, vacuolar invertase INV2, MAR-binding protein and the one putative protein showed increased expression in the low-N nutrition group. Among these proteins, the cyclophilin-like protein, which is a stress-responsive signal protein, may play pivotal roles in regulating leaf development under stress conditions. Real-time quantitative PCR analysis showed that the mRNA expression level of the cyclophilin-like protein at day 50, 60 and 70 under low-N conditions was 0.90, 1.43 and 6.9-fold higher than that under normal-N conditions, indicating that the gene expression of cyclophilin-like protein was strongly induced by low-N conditions.
authors: College of Tobacco Science, Key Laboratory for Cultivation of Tobacco Industry, Henan Agricultural University, Zhengzhou 450002, People's Republic of China.
authors: Huijuan
authors: H
authors: Yang
authors: Li
authors: L
authors: Xu
authors: Hong
authors: H
authors: Cui
authors: Boxiong
authors: B
authors: Zhong
authors: Guoshun
authors: G
authors: Liu
authors: Hongzhi
authors: H
authors: Shi
mesh_terms: D021983:Cyclophilins / Q000378:metabolism*; D005308:Fertilizers; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D009584:Nitrogen / Q000172:deficiency* / Q000378:metabolism*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000254:growth & development*; D010940:Plant Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: D005308:Fertilizers; D010940:Plant Proteins; D012333:RNA, Messenger; D021983:Cyclophilins; D009584:Nitrogen