title: DOR undergoes nucleo-cytoplasmic shuttling, which involves passage through the nucleolus.
abstract: DOR is a bi-functional protein that regulates transcription and enhances starvation-induced autophagy. While autophagy has been mostly described as a stress-response mechanism, cells also need autophagy to maintain homeostasis in basal conditions. However, the mechanisms regulating basal autophagy still remain unknown. Our results show that DOR acts in basal autophagy. Indeed, DOR already undergoes nucleo-cytoplasmic shuttling in basal conditions and, surprisingly, DOR exits continuously the nucleus and traverses the nucleolus. However, the nucleolus integrity is not essential for both DOR nucleo-cytoplasmic shuttling and DOR function on basal autophagy. Taken together, we propose that DOR exit from the nucleus is essential for basal autophagy stimulation even under nucleolus disruption.
authors: Institute for Research in Biomedicine (IRB Barcelona), Barcelona, Spain. caroline.mauvezin@gmail.com
authors: Caroline
authors: C
authors: Mauvezin
authors: Ana
authors: A
authors: Sancho
authors: Saska
authors: S
authors: Ivanova
authors: Manuel
authors: M
authors: Palacin
authors: Antonio
authors: A
authors: Zorzano
mesh_terms: D021581:Active Transport, Cell Nucleus; D001343:Autophagy; D002466:Cell Nucleolus / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D016297:Mutagenesis, Site-Directed; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C501283:TP53INP2 protein, human; C047458:fibrillarin