title: Edible oils for liver protection: hepatoprotective potentiality of Moringa oleifera seed oil against chemical-induced hepatitis in rats.
abstract: In the present study, in vitro antioxidant, antioxidative stress and hepatoprotective activity of Moringa oleifera Lam. seed oil (Ben oil; BO) was evaluated against carbon tetrachloride (CCl(4) ) induced lipid peroxidation and hepatic damage in rats. The oil at 0.2 and 0.4 mL/rat was administered orally for 21 consecutive days. The substantially elevated serum enzymatic (GOT, GPT, ALP, GGT) and bilirubin levels were significantly restored towards normalization by the oil. There was a significant elevation in the level of malondialdehyde (MDA), non-protein sulfhydryl (NP-SH), and total protein (TP) contents in the liver tissue. The results obtained indicated that BO possesses potent hepatoprotective action against CCl(4) -induced hepatic damage by lowering liver marker enzymes, MDA concentration, and elevating NP-SH and TP levels in liver tissue. The biochemical observations were supplemented with histopathological examination of rat liver. The results of this study showed that treatment with Ben oil or silymarin (as a reference) appears to enhance the recovery from hepatic damage induced by CCl(4) . The pentobarbital induced narcolepsy prolongation in mice was retarded by the Ben oil. Acute toxicity test in mice showed no morbidity or mortality. In vitro DPPH radical scavenging and β-carotene-linolic acid assay tests of the BO exhibited a moderate antioxidant activity in both tests used. The possible mechanism(s) of the liver protective activity of Ben oil activity may be due to free radical scavenging potential caused by the presence of antioxidant component(s) in the oil. Consequently, BO can be used as a therapeutic regime in treatment of some hepatic disorders.
authors: Dept of Pharmacognosy, College of Pharmacy, King Saud Univ, PO Box 2457, Riyadh 11451, Saudi Arabia.
authors: Mansour S
authors: MS
authors: Al-Said
authors: Ramzi A
authors: RA
authors: Mothana
authors: Mohammed A
authors: MA
authors: Al-Yahya
authors: Ali S
authors: AS
authors: Al-Blowi
authors: Mohammed
authors: M
authors: Al-Sohaibani
authors: Atallah F
authors: AF
authors: Ahmed
authors: Syed
authors: S
authors: Rafatullah
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001663:Bilirubin / Q000097:blood; D001713:Biphenyl Compounds / Q000494:pharmacology; D002252:Carbon Tetrachloride Poisoning / Q000473:pathology / Q000517:prevention & control; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D019787:Linoleic Acid / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D029588:Moringa oleifera / Q000737:chemistry*; D010851:Picrates / Q000494:pharmacology; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D012838:Silymarin / Q000494:pharmacology; D019207:beta Carotene / Q000032:analysis
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010938:Plant Oils; D012838:Silymarin; D019207:beta Carotene; D008315:Malondialdehyde; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D001663:Bilirubin