title: Silent TWIK-1 potassium channels conduct monovalent cation currents.
abstract: TWIK-1 two-pore domain K(+) channels generally produce nonmeasurable or very low levels of K(+) currents in heterologous expression systems under physiologically ionic conditions. Two controversial mechanisms have been proposed to account for this behavior: TWIK-1 K(+) channels are expressed in the cell surface but silenced by sumoylation at a lysine residue (TWIK-1 K274); constitutive and rapid internalization of TWIK-1 causes TWIK-1 channel silencing. Here we report that TWIK-1 K(+) channels heterologously expressed in Chinese hamster ovary cells, which are silent in physiological K(+) gradients, are able to conduct large monovalent cation currents when extracellular ionic conditions change. These results support the hypothesis that TWIK-1 K(+) channels are expressed in the cell surface but silent, and suggest that the TWIK-1 gating behavior rather than the lack of cell surface expression of TWIK-1 results in nondetectable TWIK-1 K(+) currents in heterologous expression systems.
authors: Liqun
authors: L
authors: Ma
authors: Yu-Ping
authors: YP
authors: Xie
authors: Min
authors: M
authors: Zhou
authors: Haijun
authors: H
authors: Chen
mesh_terms: D000818:Animals; D016466:CHO Cells; D002414:Cations, Monovalent / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D004553:Electric Conductivity; D005110:Extracellular Space / Q000378:metabolism; D020868:Gene Silencing; D024683:Potassium Channels, Tandem Pore Domain / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism
chemical_list: D002414:Cations, Monovalent; D024683:Potassium Channels, Tandem Pore Domain