title: Paracoccus huijuniae sp. nov., an amide pesticide-degrading bacterium isolated from activated sludge of a wastewater biotreatment system.
abstract: A facultatively anaerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-reaction-negative, coccoid to short rod-shaped strain, designated FLN-7(T), was isolated from activated sludge of a wastewater biotreatment facility. The strain was able to hydrolyse amide pesticides (e.g. diflubenzuron, propanil, chlorpropham and dimethoate) through amide bond cleavage. Strain FLN-7(T) grew at 4-42 °C (optimum 28 °C), at pH 5.0-8.0 (optimum pH 7.0) and with 0-5.0 % (w/v) NaCl (optimum 1.0 %). The major respiratory quinone was ubiquinone-10. The major cellular fatty acid was C18 : 1ω7c. The genomic DNA G+C content of strain FLN-7(T) was 66.4±0.5 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine and an unidentified glycolipid. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain FLN-7(T) was a member of the genus Paracoccus and showed highest 16S rRNA gene sequence similarities with Paracoccus aminovorans JCM 7685(T) (99.2 %), P. denitrificans DSM 413(T) (97.8 %), P. yeei CDC G1212(T) (97.3 %) and P. thiocyanatus THI 011(T) (97.1 %). Strain FLN-7(T) showed low DNA-DNA relatedness with P. aminovorans KACC 12261(T ()36.5±3.4 %), P. denitrificans KACC 12251(T) (30.5±2.6 %), P. yeei CCUG 46822(T) (26.2±2.4 %) and P. thiocyanatus KACC 13901(T) (15.5±0.9 %). Based on the phylogenetic analysis, DNA-DNA hybridization, whole-cell fatty acid composition and biochemical characteristics, strain FLN-7(T) was clearly distinguished from all recognized species of the genus Paracoccus and should be classified in a novel species, for which the name Paracoccus huijuniae sp. nov. is proposed. The type strain is FLN-7(T) ( = KACC 16242(T)  = ACCC 05690(T)).
authors: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Li-Na
authors: LN
authors: Sun
authors: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.|Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Jun
authors: J
authors: Zhang
authors: Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, RDA, Suwon 441-707, Republic of Korea.
authors: Soon-Wo
authors: SW
authors: Kwon
authors: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Jian
authors: J
authors: He
authors: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors: Shun-Gui
authors: SG
authors: Zhou
authors: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Shun-Peng
authors: SP
authors: Li
mesh_terms: D000577:Amides / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010230:Paracoccus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010575:Pesticides / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014451:Ubiquinone / Q000032:analysis; D062065:Wastewater / Q000382:microbiology
chemical_list: D000577:Amides; D004269:DNA, Bacterial; D005227:Fatty Acids; D010575:Pesticides; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014451:Ubiquinone; D062065:Wastewater; C025204:Ubiquinone Q2