title: Quantitative NMR for bioanalysis and metabolomics.
abstract: Over the last several decades, significant technical and experimental advances have made quantitative nuclear magnetic resonance (qNMR) a valuable analytical tool for quantitative measurements on a wide variety of samples. In particular, qNMR has emerged as an important method for metabolomics studies where it is used for interrogation of large sets of biological samples and the resulting spectra are treated with multivariate statistical analysis methods. In this review, recent developments in instrumentation and pulse sequences will be discussed as well as the practical considerations necessary for acquisition of quantitative NMR experiments with an emphasis on their use for bioanalysis. Recent examples of the application of qNMR for metabolomics/metabonomics studies, the characterization of biologicals such as heparin, antibodies, and vaccines, and the analysis of botanical natural products will be presented and the future directions of qNMR discussed.
authors: Department of Chemistry, University of California-Riverside, Riverside, CA 92521, USA.
authors: Gregory A
authors: GA
authors: Barding
authors: Ryan
authors: R
authors: Salditos
authors: Cynthia K
authors: CK
authors: Larive
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001688:Biological Products / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D011506:Proteins / Q000737:chemistry
chemical_list: D000596:Amino Acids; D001688:Biological Products; D011506:Proteins