title: PFGE characterisation and adhesion ability of Listeria monocytogenes isolates obtained from bovine carcasses and beef processing facilities.
abstract: Listeria monocytogenes is a pathogen capable of adhering to many surfaces and forming biofilms, which may explain its persistence in food processing environments. This study aimed to genetically characterise L. monocytogenes isolates obtained from bovine carcasses and beef processing facilities and to evaluate their adhesion abilities. DNA from 29 L. monocytogenes isolates was subjected to enzymatic restriction digestion (AscI and ApaI), and two clusters were identified for serotypes 4b and 1/2a, with similarities of 48% and 68%, respectively. The adhesion ability of the isolates was tested considering: inoculum concentration, culture media, carbohydrate source, NaCl concentration, incubation temperature, and pH. Each isolate was tested at 10⁸ CFU mL⁻¹ and classified according to its adhesion ability as weak (8 isolates), moderate (17) or strong (4). The isolates showed higher adhesion capability in non-diluted culture media, media at pH 7.0, incubation at 25°C and 37 °C, and media with NaCl at 5% and 7%. No relevant differences were observed for adhesion ability with respect to the carbohydrate source. The results indicated a wide diversity of PFGE profiles of persistent L. monocytogenes isolates, without relation to their adhesion characteristics. Also, it was observed that stressing conditions did not enhance the adhesion profile of the isolates.
authors: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors: Newton Nascentes
authors: NN
authors: Galvão
authors: Eb
authors: E
authors: Chiarini
authors: Maria Teresa
authors: MT
authors: Destro
authors: Márcia
authors: M
authors: de Aguiar Ferreira
authors: Luís Augusto
authors: LA
authors: Nero
mesh_terms: D000003:Abattoirs; D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001938:Brazil; D002417:Cattle; D016521:Electrophoresis, Gel, Pulsed-Field; D004783:Environmental Microbiology; D005517:Foodborne Diseases / Q000517:prevention & control; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D008088:Listeriosis / Q000517:prevention & control; D008460:Meat / Q000382:microbiology*; D050296:Microbial Viability; D058889:Molecular Typing; D010802:Phylogeny; D012462:Saline Solution, Hypertonic; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D012462:Saline Solution, Hypertonic