title: In situ electrostimulation drives a regenerative shift in the zone of infarcted myocardium.
abstract: Electrostimulation represents a well-known trophic factor for different tissues. In vitro electrostimulation of non-stem and stem cells induces myogenic predifferentiation and may be a powerful tool to generate cells with the capacity to respond to local areas of injury. We evaluated the effects of in vivo electrostimulation on infarcted myocardium using a miniaturized multiparameter implantable stimulator in rats. Parameters of electrostimulation were organized to avoid a direct driving or pacing of native heart rhythm. Electrical stimuli were delivered for 14 days across the scar site. In situ electrostimulation used as a cell-free, cytokine-free stimulation system, improved myocardial function, and increased angiogenesis through endothelial progenitor cell migration and production of vascular endothelial growth factor (VEGF). In situ electrostimulation represents a novel means to stimulate repair of the heart and other organs, as well as to precondition tissues for treatment with cell-based therapies.
authors: Center for Integrated Research, Department of Cardiovascular Science, Unit of Cardiac Surgery, Università Campus Bio-Medico di Roma, Rome, Italy.
authors: Cristiano
authors: C
authors: Spadaccio
authors: Alberto
authors: A
authors: Rainer
authors: Federico
authors: F
authors: De Marco
authors: Mario
authors: M
authors: Lusini
authors: Paolo
authors: P
authors: Gallo
authors: Pietro
authors: P
authors: Sedati
authors: Andrea Onetti
authors: AO
authors: Muda
authors: Stefano
authors: S
authors: De Porcellinis
authors: Chiara
authors: C
authors: Gregorj
authors: Giuseppe
authors: G
authors: Avvisati
authors: Marcella
authors: M
authors: Trombetta
authors: Massimo
authors: M
authors: Chello
authors: Elvio
authors: E
authors: Covino
authors: David A
authors: DA
authors: Bull
authors: Amit N
authors: AN
authors: Patel
authors: Jorge A
authors: JA
authors: Genovese
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement; D064987:Cell- and Tissue-Based Therapy; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D009203:Myocardial Infarction / Q000378:metabolism / Q000503:physiopathology* / Q000628:therapy; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology; D018919:Neovascularization, Physiologic; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014057:Tomography, X-Ray Computed; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D016277:Ventricular Function, Left / Q000502:physiology
chemical_list: D042461:Vascular Endothelial Growth Factor A; D009320:Atrial Natriuretic Factor