title: Evaluation on cytotoxicity and genotoxicity of the L-glutamic acid coated iron oxide nanoparticles.
abstract: To evaluate the cytotoxicity and genotoxicity of L-glutamic acid (Glu) coated Fe2O3 nanoparticles (hereafter refer as Glu@MNPs) on Chinese Hamster Lung (CHL) cells using Trypan blue dye exclusion assay, Oxidative stress markers, Comet assay and micronucleus (MN) assay. Results showed a low cytotoxicity with an IC50 was 254.739 microg/ml 36 h post incubation period in CHL cells. Furthermore, Cell redox status is slightly disturbed: Glu@MNPs exposure cause reactive oxygen species production, glutathione depletion and inactivation of some antioxidant enzymes: glutathione reductase, superoxide dismutase, but not catalase. Moreover, no significant genotoxic response was observed in CHL cells over concentration ranges from 8 to 128 microg/mL for all exposure time periods. The results suggest that the Glu@MNPs show biocompatibility In Vitro.
authors: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, China.
authors: Ting
authors: T
authors: Zhang
authors: Li
authors: L
authors: Qian
authors: Meng
authors: M
authors: Tang
authors: Yuying
authors: Y
authors: Xue
authors: Lu
authors: L
authors: Kong
authors: Shanshan
authors: S
authors: Zhang
authors: Yuepu
authors: Y
authors: Pu
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D020552:Comet Assay; D006224:Cricetinae; D005290:Ferric Compounds / Q000737:chemistry*; D018698:Glutamic Acid / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000633:toxicity*; D015162:Micronucleus Tests; D046529:Microscopy, Electron, Transmission
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D018698:Glutamic Acid