title: Oriented nucleation of hemozoin at the digestive vacuole membrane in Plasmodium falciparum.
abstract: Heme detoxification is a critical step in the life cycle of malaria-causing parasites, achieved by crystallization into physiologically insoluble hemozoin. The mode of nucleation has profound implications for understanding the mechanism of action of antimalarial drugs that inhibit hemozoin growth. Several lines of evidence point to involvement of acylglycerol lipids in the nucleation process. Hemozoin crystals have been reported to form within lipid nanospheres; alternatively, it has been found in vitro that they are nucleated at an acylglycerol lipid-water interface. We have applied cryogenic soft X-ray tomography and three-dimensional electron microscopy to address the location and orientation of hemozoin crystals within the digestive vacuole (DV), as a signature of their nucleation and growth processes. Cryogenic soft X-ray tomography in the "water window" is particularly advantageous because contrast generation is based inherently on atomic absorption. We find that hemozoin nucleation occurs at the DV inner membrane, with crystallization occurring in the aqueous rather than lipid phase. The crystal morphology indicates a common {100} orientation facing the membrane as expected of templated nucleation. This is consistent with conclusions reached by X-ray fluorescence and diffraction in a companion work. Uniform dark spheres observed in the parasite were identified as hemoglobin transport vesicles. Their analysis supports a model of hemozoin nucleation primarily in the DV. Modeling of the contrast at the DV membrane indicates a 4-nm thickness with patches about three times thicker, possibly implicated in the nucleation.
authors: Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot 76100, Israel.
authors: Sergey
authors: S
authors: Kapishnikov
authors: Allon
authors: A
authors: Weiner
authors: Eyal
authors: E
authors: Shimoni
authors: Peter
authors: P
authors: Guttmann
authors: Gerd
authors: G
authors: Schneider
authors: Noa
authors: N
authors: Dahan-Pasternak
authors: Ron
authors: R
authors: Dzikowski
authors: Leslie
authors: L
authors: Leiserowitz
authors: Michael
authors: M
authors: Elbaum
mesh_terms: D000042:Absorption; D000818:Animals; D018360:Crystallography, X-Ray / Q000379:methods; D004912:Erythrocytes / Q000469:parasitology; D006418:Heme / Q000737:chemistry; D006420:Hemeproteins / Q000737:chemistry*; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D007477:Ions; D008055:Lipids / Q000737:chemistry; D008854:Microscopy, Electron / Q000379:methods; D054118:Nanospheres / Q000737:chemistry; D010963:Plasmodium falciparum / Q000378:metabolism*; D011804:Quinolines / Q000737:chemistry; D013499:Surface Properties; D014617:Vacuoles / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D006420:Hemeproteins; D007477:Ions; D008055:Lipids; D011804:Quinolines; D014867:Water; C020753:hemozoin; D006418:Heme; C037219:quinoline