title: Low-intensity pulsed ultrasound-mediated stimulation of hematopoietic stem/progenitor cell viability, proliferation and differentiation in vitro.
abstract: Low-intensity pulsed ultrasound (LIPUS) stimulated the viability, proliferation and differentiation of hematopoietic stem/progenitor cells (HSPC) from fresh and cryopreserved peripheral blood leukapheresis product, as well as cord blood when applied for 10 min each day for 4 days. Cell viability, proliferation and differentiation were assessed on day 5 by viable cell counting, MTS proliferation assay, flow cytometry, and colony-forming unit assay. LIPUS stimulation: (i) enhanced the proliferation of fresh HSPC and maintained the viability of cryopreserved HSPC in vitro; (ii) did not affect the percentage of CD34(+) and CD14(+) cells; and (iii) enhanced burst-forming unit-erythroid colony formation. Hence, we suggest that this novel LIPUS stimulation approach might enhance the efficacy of clinical transplantation and cellular therapies using HSPC.
authors: ECERF W6-019, Department of Biomedical Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada. px1@ualberta.ca
authors: Peng
authors: P
authors: Xu
authors: Hilal
authors: H
authors: Gul-Uludag
authors: Woon T
authors: WT
authors: Ang
authors: Xiaoyan
authors: X
authors: Yang
authors: Min
authors: M
authors: Huang
authors: Leah
authors: L
authors: Marquez-Curtis
authors: Locksley
authors: L
authors: McGann
authors: Anna
authors: A
authors: Janowska-Wieczorek
authors: James
authors: J
authors: Xing
authors: Eric
authors: E
authors: Swanson
authors: Jie
authors: J
authors: Chen
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D002454:Cell Differentiation / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005312:Fetal Blood / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000528:radiation effects*; D006801:Humans; D007937:Leukapheresis; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D012107:Research Design; D013016:Sound
chemical_list: D018952:Antigens, CD34; D018950:Lipopolysaccharide Receptors