title: Real-time monitoring of strand-displacement DNA amplification by a contactless electrochemical microsystem using interdigitated electrodes.
abstract: This paper reports the design and implementation of a contactless conductivity detection system which combines a thermal control cell, a data processing system and an electrochemical (EC) cell for label-free isothermal nucleic acid amplification and real-time monitoring. The EC cell consists of a microchamber and interdigitated electrodes as the contactless conductivity biosensor with a cover slip as insulation. In our work, contactless EC measurements, the effects of trehalose on amplification, and chip surface treatment are investigated. With the superior performance of the biosensor, the device can detect the amount of pure DNA at concentrations less than 0.1 pg μl(-1). The EC cell, integrated with a heater and a temperature sensor, has successfully implemented nicking-based strand-displacement amplification at an initial concentration of 2.5 μM and the yields are monitored directly (dismissing the use of probes or labels) on-line. This contactless detector carries important advantages: high anti-interference capability, long detector life, high reusability and low cost. In addition, the small size, low power consumption and portability of the detection cell give the system the potential to be highly integrated for use in field service and point of care applications.
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information TechnologyChinese Academy of Science, China.
authors: Xinxin
authors: X
authors: Fang
authors: Huanqian
authors: H
authors: Zhang
authors: Feng
authors: F
authors: Zhang
authors: Fengxiang
authors: F
authors: Jing
authors: Hongju
authors: H
authors: Mao
authors: Qinghui
authors: Q
authors: Jin
authors: Jianlong
authors: J
authors: Zhao
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D004247:DNA / Q000032:analysis*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D013696:Temperature; D014199:Trehalose / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D004247:DNA; D019904:Polymethyl Methacrylate; D014199:Trehalose