title: AtPAP2 is a tail-anchored protein in the outer membrane of chloroplasts and mitochondria.
abstract: To date, Arabidopsis purple acid phosphatase 2 (AtPAP2) is the only known plant protein that is dual-targeted to chloroplasts and mitochondria by a C-terminal targeting signal. Using in vitro organelle import and green fluorescence protein (GFP) localization assays, we showed that AtPAP2 is located on, but not imported across the outer membrane (OM) of chloroplasts and mitochondria and exposed its N-terminal enzymatic domain to the cytosol. It was also found that a short stretch of 30 amino acids (a.a.) at the C-terminal region (a.a. 615-644) that contains a stretch of 18 hydrophobic residues, a WYAK motif and 8 hydrophilic residues is sufficient for dual-targeting. Mutation of WYAK to WYAE had no effect on dual-targeting ability suggesting that the charge within this flanking region alone is not an important determinant for dual-targeting.
authors: School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors: Feng
authors: F
authors: Sun
authors: Chris
authors: C
authors: Carrie
authors: Simon
authors: S
authors: Law
authors: Monika W
authors: MW
authors: Murcha
authors: Renshan
authors: R
authors: Zhang
authors: Yee Song
authors: YS
authors: Law
authors: Pui Kit
authors: PK
authors: Suen
authors: James
authors: J
authors: Whelan
authors: Boon Leong
authors: BL
authors: Lim
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism; D012275:Oryza / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D010447:Peptide Hydrolases / Q000494:pharmacology; D030821:Plants, Genetically Modified; D021382:Protein Sorting Signals; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C514560:PAP2 protein, Arabidopsis; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D010447:Peptide Hydrolases