title: Dietary protein restriction and excess of pregnant German Landrace sows induce changes in hepatic gene expression and promoter methylation of key metabolic genes in the offspring.
abstract: Maternal nutrition during gestation has important effects on offspring gene expression mediated by DNA methylation. In order to evaluate the effect of restricted and excess protein intake during gestation, hepatic gene expression and DNA methylation of key metabolic genes NR3C1, PPARα, HMGCR, PGC1α, INSR and CYP2C34 were investigated. Liver samples of German Landrace offspring were collected at Gestational Day 95, at birth, at weaning and from finisher pigs. Gene expression in foetal liver revealed significant differences between the control group (CO) and the low-protein group (LP) in HMGCR (P<.0001), INSR (P=.0003), NR3C1 (P=.020) and PGC1α (P=.003). At birth INSR (P=.032), PPARα (P=.0006) and CYP2C34 (P<.0001) showed significant differences between LP and CO. CYP2C34 was significantly increased in the high-protein group (HP) compared to CO (P=.001). At weaning, INSR was significantly higher expressed in LP than in CO (P=.018). HMGCR showed a significant decrease of transcript amount in HP compared to CO (P=.0006). Furthermore, we studied the question whether gene expression differences between distinct diet groups are a result of differential DNA methylation status. CpG sites in the 5'-flanking region of CYP2C34 showed a significant positive correlation with transcript amount in LP (nt -137: R=0.67, P<.0001; nt -112: R=0.54, P=.003). In NR3C1 methylation, differences in the CpG island were negatively correlated with gene expression data in LP (R=-0.34, P=.032). The mean of methylation of PPARα over CpG sites from nt -220 to -11 was significantly increased in the LP group compared with CO (P=.043). These data suggest an influence of DNA methylation in nutrient-dependent transcriptional regulation of NR3C1, PPARα and CYP2C34.
authors: Research Group Functional Genomics, Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany.
authors: Simone
authors: S
authors: Altmann
authors: Eduard
authors: E
authors: Murani
authors: Manfred
authors: M
authors: Schwerin
authors: Cornelia C
authors: CC
authors: Metges
authors: Klaus
authors: K
authors: Wimmers
authors: Siriluck
authors: S
authors: Ponsuksili
mesh_terms: D024506:5' Flanking Region; D000818:Animals; D001483:Base Sequence; D018899:CpG Islands; D019175:DNA Methylation; D018753:Diet, Protein-Restricted / Q000009:adverse effects; D004044:Dietary Proteins / Q000494:pharmacology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics; D008099:Liver / Q000187:drug effects* / Q000196:embryology; D039382:Maternal Nutritional Physiological Phenomena; D008969:Molecular Sequence Data; D047493:PPAR alpha / Q000235:genetics; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011401:Promoter Regions, Genetic; D011972:Receptor, Insulin / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000235:genetics; D034421:Sus scrofa / Q000196:embryology / Q000235:genetics
chemical_list: D004044:Dietary Proteins; D047493:PPAR alpha; D011965:Receptors, Glucocorticoid; D006903:Hydroxymethylglutaryl CoA Reductases; D011972:Receptor, Insulin