title: Benzodiazepines and metabolites from biological fluids by liquid chromatography electrospray tandem mass spectrometry.
abstract: Solid-phase extraction and liquid chromatography-tandem mass spectrometry are invaluable techniques for the determination of benzodiazepines and metabolites in biological matrices. The reason for using tandem mass spectrometry is to increase limits of detection without the need for chemical derivatization. Here we describe a technique for the detection of 26 benzodiazepines and metabolites at a detection limit of approximately 1-2 ng/mL in blood and 1-5 ng/mL in urine when screened using a data-dependent scan method.
authors: Chemistry Unit, FBI Laboratory, Quantico, VA, USA. Cynthia.Morris-Kukoski@ic.fbi.gov
authors: Cynthia L
authors: CL
authors: Morris-Kukoski
authors: Jason E
authors: JE
authors: Schaff
authors: Louis J
authors: LJ
authors: Reda
mesh_terms: D001569:Benzodiazepines / Q000097:blood* / Q000378:metabolism / Q000652:urine*; D001826:Body Fluids / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D001569:Benzodiazepines