title: Novel microsatellite loci for Agave parryi and cross-amplification in Agave palmeri (Agavaceae).
abstract: OBJECTIVE
  To examine the foraging behavior of nectarivorous bats in southeastern Arizona, we developed microsatellite primers in Agave parryi. These markers were also tested for cross-amplification and applicability to assess patterns of genetic diversity and structure in A. palmeri.
  
  
  RESULTS
  Utilizing DNA sequence data from 454 shotgun sequencing, we identified seven novel polymorphic microsatellite loci in A. parryi and screened them for cross-amplification in A. palmeri. These markers were characterized in two populations of 30 individuals each for each species. In A. parryi, all primers were polymorphic and amplified between three and 12 alleles per population. In A. palmeri, all primers amplified, six were polymorphic, and allelic diversity ranged from one to 16 alleles per population.
  
  
  CONCLUSIONS
  Our results demonstrate the applicability of these microsatellite primers for population genetics studies in both A. parryi and A. palmeri.
authors: Environmental Laboratory, U.S. Army Engineer Research and Development Center, 3909 Halls Ferry Road, Vicksburg, MS 39180, USA. denise.l.lindsay@usace.army.mil
authors: Denise L
authors: DL
authors: Lindsay
authors: Christine E
authors: CE
authors: Edwards
authors: Michael G
authors: MG
authors: Jung
authors: Pamela
authors: P
authors: Bailey
authors: Richard F
authors: RF
authors: Lance
mesh_terms: D027882:Agave / Q000235:genetics*; D000818:Animals; D001130:Arizona; D002685:Chiroptera; D018744:DNA, Plant / Q000235:genetics; D060434:Herbivory; D018895:Microsatellite Repeats
chemical_list: D018744:DNA, Plant