title: Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator.
abstract: In this report, a Ti:Sapphire oscillator was utilized to realize synchronization-free stimulated emission depletion (STED) microscopy. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. With synchronization-free STED, we imaged 200 nm nanospheres as well as all three cytoskeletal elements (microtubules, intermediate filaments, and actin filaments), clearly demonstrating the resolving power of synchronization-free STED over conventional diffraction limited imaging. It also allowed us to discover that, Dylight 650, exhibits improved performance over ATTO647N, a fluorophore frequently used in STED. Furthermore, we applied synchronization-free STED to image fluorescently-labeled intracellular viral RNA granules, which otherwise cannot be differentiated by confocal microscopy. Thanks to the widely available Ti:Sapphire oscillators in multiphoton imaging system, this work suggests easier access to setup super-resolution microscope via the synchronization-free STED.
authors: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
authors: Yujia
authors: Y
authors: Liu
authors: Yichen
authors: Y
authors: Ding
authors: Eric
authors: E
authors: Alonas
authors: Wenli
authors: W
authors: Zhao
authors: Philip J
authors: PJ
authors: Santangelo
authors: Dayong
authors: D
authors: Jin
authors: James A
authors: JA
authors: Piper
authors: Junlin
authors: J
authors: Teng
authors: Qiushi
authors: Q
authors: Ren
authors: Peng
authors: P
authors: Xi
mesh_terms: D003599:Cytoskeleton / Q000737:chemistry; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D053758:Nanoparticles; D014025:Titanium
chemical_list: D014025:Titanium