title: γδ T lymphocytes are recruited into the inflamed uterus of bitches suffering from pyometra.
abstract: Very little is known about the occurrence of immune system cells in the canine uterus. The aim of this study was to generate information about lymphocyte subsets that are present in the healthy canine uterus and that are recruited under inflammatory conditions caused by pyometra. Using immunohistochemistry and flow cytometry, a significant influx of γδ T lymphocytes was found in pyometra samples mainly due to recruitment of γδ(+)/CD8(-) T lymphocytes. The relative expression of genes encoding selected cytokines/chemokines was evaluated in samples from healthy and pyometra-affected uteri. Expression of pro-inflammatory cytokines (including IL-1β, TNF-α, IL-8, IL-17 and IFN-γ) and chemokines (including CXCL10, CCL4 and CCL5) was upregulated in pyometra samples confirming the presence of inflammation. In contrast, the expression of the homeostatic chemokine CCL25 and of the anti-inflammatory cytokine IL-10 was downregulated and unchanged, respectively.
authors: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
authors: A
authors: A
authors: Bartoskova
authors: P
authors: P
authors: Turanek-Knotigova
authors: J
authors: J
authors: Matiasovic
authors: Z
authors: Z
authors: Oreskovic
authors: M
authors: M
authors: Vicenova
authors: H
authors: H
authors: Stepanova
authors: P
authors: P
authors: Ondrackova
authors: R
authors: R
authors: Vitasek
authors: L
authors: L
authors: Leva
authors: P F
authors: PF
authors: Moore
authors: M
authors: M
authors: Faldyna
mesh_terms: D000818:Animals; D018925:Chemokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D004283:Dog Diseases / Q000276:immunology* / Q000378:metabolism; D004285:Dogs / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000662:veterinary; D055112:Pyometra / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D014599:Uterus / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology
chemical_list: D018925:Chemokines; D016207:Cytokines