title: Biocompatibility of CS-PPy nanocomposites and their application to glucose biosensor.
abstract: The intrinsic properties and application potential of nanocolloids are mainly determined by size, shape, composition, and structure. In this case, a novel glucose biosensor was developed by using the chitosan-polypyrrole (CS-PPy) nanocomposites as special modified materials that coating onto the surface of glassy carbon electrode (GCE). The CS-PPy nanocomposites were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM), respectively. Moreover, the interaction of CS-PPy nanocomposites with glucose oxidase (GOD) was also investigated by the combined studies with Fourier transform infrared spectroscopy (FTIR) and circular dichroism spectroscopy (CD). Due to the conductivity of polypyrrole (PPy), good biocompatibility of CS, and advantages of nanoparticles, CS-PPy nanocomposites were chosen and designed to modify the GCE for the retention of GOD's biological activity and the vantage of electron transfer between GOD and electrodes. The GOD biosensor exhibited a fast amperometric response (5s) to glucose, a good linear current-time relation over a wide range of glucose concentrations from 5.00×10(-4) to 1.47×10(-1)M, and a low detection limit of 1.55×10(-5)M. The GOD biosensor modified with CS-PPy nanocomposites will have essential meaning and practical application in future that attributed to the simple method of fabrication and good performance.
authors: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, PR China.
authors: Yi
authors: Y
authors: Fang
authors: Yalong
authors: Y
authors: Ni
authors: Guohui
authors: G
authors: Zhang
authors: Chun
authors: C
authors: Mao
authors: Xiaohua
authors: X
authors: Huang
authors: Jian
authors: J
authors: Shen
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D053761:Nanocomposites / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011487:Protein Conformation; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D002244:Carbon; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose