title: Oropharyngeal malignant epithelial cell, lymphocyte and macrophage CD44 surface receptors for hyaluronate are expressed in sustained EBV infection: immunohistochemical data and EBV DNA tissue indices.
abstract: The role of CD44 in Epstein-Barr virus (EBV)-related epithelial tumors is poorly understood. We studied the expression of CD44 in EBV infection in patients with oral squamous cell carcinoma (SCC) and nasopharyngeal carcinoma (NPC) and measured the EBV DNA. Whole blood, plasma and tissue samples from 8 male and 2 female patients with oral SCC, NPC, salivary gland lymphoepithelioma, normal salivary gland and buccal mucosa were assayed for EBV DNA. Expression of CD44, latent membrane protein (LMP), and labeling of lymphocytes, macrophages and dendritic cells were estimated by immunohistochemistry. Tissue EBV DNA was detected in 7 of 8 cases (87.5%) of oral malignant, benign and border-line lesions. LMP expression levels in tumors varied from absence and minimal to moderate - 50.3, 43.6, 6.0% and 91.1, 6.7, 2.2% for SCC and NPC, respectively. Levels of CD44 positivity in neoplasms were minimal (15.5 and 16.7%), moderate (30.3 and 47.8%), and diffuse (54.2 and 35.5%) for SCC and NPC, respectively, thus deviating from normal oral mucosa revealing heavily stained (100.0%) epithelial contours. CD19-positive B lymphocytes and S100-positive dendritic cells were intermixed with neoplastic cells. Collectively, CD44 mediated signaling may be implicated in EBV infection associated with the pathogenesis of oral SCC and NPC.
authors: Riga Stradins University, Institute of Anatomy and Anthropology, 16, Dzirciema Str., Riga LV 1007, Latvia. AAISEL@rsu.lv
authors: Valerija
authors: V
authors: Groma
authors: Anna
authors: A
authors: Kazanceva
authors: Zaiga
authors: Z
authors: Nora-Krukle
authors: Modra
authors: M
authors: Murovska
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics*; D003713:Dendritic Cells / Q000378:metabolism / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology; D020031:Epstein-Barr Virus Infections / Q000378:metabolism* / Q000821:virology; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism / Q000821:virology; D008264:Macrophages / Q000378:metabolism / Q000821:virology; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism / Q000821:virology; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000821:virology; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000821:virology; D012469:Salivary Glands / Q000378:metabolism / Q000821:virology; D015398:Signal Transduction; D019562:Viral Load; D014763:Viral Matrix Proteins / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C497483:CD44 protein, human; D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D018960:Hyaluronan Receptors; D014763:Viral Matrix Proteins; D006820:Hyaluronic Acid