title: Mechanisms of toxicity induced by SiO2 nanoparticles of in vitro human alveolar barrier: effects on cytokine production, oxidative stress induction, surfactant proteins A mRNA expression and nanoparticles uptake.
abstract: An in vitro human alveolar barrier established by the coculture of epithelial human cell line NCI-H441 with endothelial human cell line ISO-HAS1 was used to evaluate the effects of amorphous silicon dioxide nanoparticles (SiNPs), in the presence or absence of THP-1 cells (monocytes). SiNPs exposure induced production of proinflammatory cytokine and oxidative stress. A high release of TNF-α and IL-8 by epithelial/endothelial cells, potentiated in the presence of THP-1 cells could contribute to the observed downregulation of surfactant proteins A mRNA expression resulting in the damage of the alveolar barrier. The obtained results suggested that in vitro approach can be used to study pulmonary toxicity as long as the applied in vitro model mimics closely the complexity of in vivo situation.
authors: Institute for Health and Consumer Protection, Joint Research Centre, European Commission, Validation of Alternative Methods Unit / EURL ECVAM , via E. Fermi 2749, Ispra VA, Italy.
authors: Lucian Romeo
authors: LR
authors: Farcal
authors: Chiara
authors: C
authors: Uboldi
authors: Dora
authors: D
authors: Mehn
authors: Guido
authors: G
authors: Giudetti
authors: Paola
authors: P
authors: Nativo
authors: Jessica
authors: J
authors: Ponti
authors: Douglas
authors: D
authors: Gilliland
authors: François
authors: F
authors: Rossi
authors: Anna
authors: A
authors: Bal-Price
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D016207:Cytokines / Q000378:metabolism*; D017097:Electric Impedance; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides; D008264:Macrophages / Q000502:physiology; D009000:Monocytes / Q000502:physiology; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D011650:Pulmonary Alveoli / Q000187:drug effects*; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species; D012822:Silicon Dioxide / Q000737:chemistry / Q000633:toxicity*
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D037662:Pulmonary Surfactant-Associated Protein A; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D012822:Silicon Dioxide