title: HP1a targets the Drosophila KDM4A demethylase to a subset of heterochromatic genes to regulate H3K36me3 levels.
abstract: The KDM4 subfamily of JmjC domain-containing demethylases mediates demethylation of histone H3K36me3/me2 and H3K9me3/me2. Several studies have shown that human and yeast KDM4 proteins bind to specific gene promoters and regulate gene expression. However, the genome-wide distribution of KDM4 proteins and the mechanism of genomic-targeting remain elusive. We have previously identified Drosophila KDM4A (dKDM4A) as a histone H3K36me3 demethylase that directly interacts with HP1a. Here, we performed H3K36me3 ChIP-chip analysis in wild type and dkdm4a mutant embryos to identify genes regulated by dKDM4A demethylase activity in vivo. A subset of heterochromatic genes that show increased H3K36me3 levels in dkdm4a mutant embryos overlap with HP1a target genes. More importantly, binding to HP1a is required for dKDM4A-mediated H3K36me3 demethylation at a subset of heterochromatic genes. Collectively, these results show that HP1a functions to target the H3K36 demethylase dKDM4A to heterochromatic genes in Drosophila.
authors: Stowers Institute for Medical Research, Kansas City, Missouri, United States of America.
authors: Chia-Hui
authors: CH
authors: Lin
authors: Ariel
authors: A
authors: Paulson
authors: Susan M
authors: SM
authors: Abmayr
authors: Jerry L
authors: JL
authors: Workman
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology*; D004330:Drosophila / Q000201:enzymology*; D029721:Drosophila Proteins / Q000378:metabolism* / Q000502:physiology*; D006570:Heterochromatin / Q000235:genetics*; D056466:Histone Demethylases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; D006570:Heterochromatin; D006657:Histones; C452193:heterochromatin protein 1, Drosophila; D056466:Histone Demethylases; C534109:KDM4A protein, Drosophila