title: Dietary curcumin inhibits atherosclerosis by affecting the expression of genes involved in leukocyte adhesion and transendothelial migration.
abstract: METHODS
  The aim of the study was to examine the atheroprotective effect of dietary curcumin in a mouse model of atherosclerosis and to identify its cellular and molecular targets at the vascular level.
  
  
  RESULTS
  ApoE(-/-)  mice were fed with curcumin at 0.2% (wt/wt) in diet for 4 months. This supplementation reduced the extent of atherosclerotic lesion by 26% and induced changes in expression of genes implicated in cell adhesion and transendothelial migration or cytoskeleton organization, as revealed by a transcriptomic analysis in the aorta. Expression profile of these genes suggests reduction in both leukocyte adhesion and transendothelial migration. In agreement with this hypothesis, we observed a reduction (-37%) in macrophage infiltration in the plaque, as measured by immunohistochemistry, and, in vitro, a lower adhesion of monocytes to TNF-α-stimulated endothelial cells (-32%) after exposure to a nutritionally achievable concentration of curcumin. These changes in gene expression could be related to the observed increased expression of IκB protein and decrease of TNF-α-induced NF-κB/DNA binding and NF-κB-transcriptional activity upon exposure to curcumin.
  
  
  CONCLUSIONS
  Our findings pointed out that the antiatherogenic effect of curcumin could be linked to its effect on gene networks and cell functions related to leukocyte adhesion and transendothelial migration via NF-κB-dependent pathways.
authors: INRA, UMR 1019, UNH, CRNH Auvergne, Clermont-Ferrand, France.
authors: Dilek
authors: D
authors: Coban
authors: Dragan
authors: D
authors: Milenkovic
authors: Audrey
authors: A
authors: Chanet
authors: Jamila
authors: J
authors: Khallou-Laschet
authors: Linde
authors: L
authors: Sabbe
authors: Ajay
authors: A
authors: Palagani
authors: Wim
authors: W
authors: Vanden Berghe
authors: Andrzej
authors: A
authors: Mazur
authors: Christine
authors: C
authors: Morand
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects; D001057:Apolipoproteins E / Q000235:genetics; D050197:Atherosclerosis / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D003474:Curcumin / Q000494:pharmacology*; D019587:Dietary Supplements; D042783:Endothelial Cells / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D020672:I-kappa B Proteins / Q000235:genetics; D007962:Leukocytes / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008817:Mice, Mutant Strains; D009000:Monocytes / Q000166:cytology / Q000187:drug effects; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D057705:Transendothelial and Transepithelial Migration / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D001057:Apolipoproteins E; D020672:I-kappa B Proteins; D016328:NF-kappa B; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; D003474:Curcumin