title: Wrapping tissues with a pre-established cage-like layer composed of living cells.
abstract: We report a method for wrapping tissues with a pre-established cage-like layer composed of living cells. We encapsulated multicellular aggregates of human hepatoma HepG2 cells as a model of tissues such as pancreatic islets and hepatocyte spheroids in alginate-based hydrogel microcapsules and subsequently coated the microcapsule surface with a gelatin derivative through a horseradish peroxidase-catalyzed reaction. Human aorta endothelial (HAE) cells grew on the surface and formed a cell layer within 24 h of incubating the microcapsules in a medium containing the cells. Subsequent degradation of the hydrogel microcapsule using a non-proteolytic enzyme, alginate lyase, resulted in a cage-like structure of HAE cells formed around the microcapsule. The HAE cell layer shrank without fragmenting and wrapped the inner spherical tissue. This method was also effective for wrapping multiple cellular aggregates within a single cage of HAE cells. In addition, it was possible to wrap tissue grown from individual cells in spherical cavities within the microcapsules.
authors: Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan. sakai@cheng.es.osaka-u.ac.jp
authors: Shinji
authors: S
authors: Sakai
authors: Hitomi
authors: H
authors: Inagaki
authors: Kazuya
authors: K
authors: Inamoto
authors: Masahito
authors: M
authors: Taya
mesh_terms: D000464:Alginates / Q000737:chemistry; D001011:Aorta / Q000166:cytology; D001672:Biocompatible Materials / Q000737:chemistry*; D049109:Cell Proliferation; D002470:Cell Survival; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000737:chemistry / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D056945:Hep G2 Cells; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D007515:Islets of Langerhans / Q000166:cytology; D011133:Polysaccharide-Lyases / Q000737:chemistry; D018874:Spheroids, Cellular / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D014184:Transplantation, Homologous / Q000639:trends
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid; D005780:Gelatin; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase