title: Inhibitory effect of a Ras homologue member I on pancreatic cancer and its modulation on epithelium growth factor receptor-Ras-Raf-mitogen-activated protein kinase/extracellular regulated protein kinase/1/2 pathway.
abstract: OBJECTIVE
  To investigate the regulation of epithelium growth factor receptor (EGFR), pan-Ras, and extracellular regulated protein kinase (ERK) with both a ras homologue member I (ARHI) suppression and epithelium growth factor (EGF) stimulation.
  
  
  METHODS
  After identification and implication, the constructed plasmid pIRES2-EGFP-ARHI was transfected into Panc-1. The untransfected cell was also explored as controls. The growth curve was drawn to indicate the proliferation effect of ARHI. EGFR-ELISA was performed to investigate the expression of EGFR. Western blot analysis was used to investigate the expression of protein MAPK/ERK1/2, pan-Ras in Panc-1.
  
  
  RESULTS
  The proliferation rate of Panc-1 was inhibited by ARHI compared with both empty plasmid and untransfected cell. The amount of EGFR was parallel in both transfected and untransfected cell but affected by EGF stimulation. The amount of pan-Ras was decreased after ARHI transfection. The optimum concentration of EGF effect on P-ERK was 50 ng/ml.
  
  
  CONCLUSIONS
  Both ARHI and EGF play roles in the EGF-EGFR-Ras-Raf-MAPK/ERK1/2 pathway.
authors: Department of Gastroenterology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors: Jia
authors: J
authors: Lu
authors: Jia-ming
authors: JM
authors: Qian
authors: Hong
authors: H
authors: Yang
authors: Jing-nan
authors: JN
authors: Li
authors: Feng-ji
authors: FJ
authors: Xu
mesh_terms: D049109:Cell Proliferation; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction; D014162:Transfection; D014407:Tumor Cells, Cultured; D018631:ras Proteins / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000235:genetics*
chemical_list: C117386:DIRAS3 protein, human; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D048052:Mitogen-Activated Protein Kinase 3; D018631:ras Proteins; D020741:rho GTP-Binding Proteins