title: [Effects of megsin gene transfection on the expressions   of MCP-1 and ICAM-1 in mouse glomerular mesangial cells].
abstract: OBJECTIVE
  To observe the effects of megsin gene transfection on the expressions  of monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1(ICAM-1).
  
  
  METHODS
  Mouse glomerular mesangial cells were cultured in high glucose medium, and  then cell proliferation was measured by MTT assay at 12, 24 and 48 h respectively. The expressions  of megsin, MCP-1 and ICAM-1 in mesangial cells were detected by immunocytochemical staining and Western blotting.  The concentration of type IV collagen in  the culture  supernatant of mesangial cells was measured by ELISA.
  
  
  RESULTS
  Under high glucose concentration, the expressions  of megsin, MCP-1 and ICAM-1  increased;  the concentration of type IV collagen in the cell supernatant was elevated as well;  and mesangial cell proliferation was enhanced. After megsin gene transfection, the above changes were more significant,  but  were abated following megsin shRNA/transfection.
  
  
  CONCLUSIONS
  Megsin gene can up-regulate the expressions  of MCP-1 and ICAM-1, promote  mesangial cell proliferation and mesangial extracellular matrix accumulation.
authors: Department of Nephrology, Third Hospital of Hebei Medical University, Shijiazhuang 050051, China. gwliujie@126.com
authors: Jie
authors: J
authors: Liu
authors: Ying
authors: Y
authors: Li
authors: Mao-dong
authors: MD
authors: Liu
authors: Guang-wei
authors: GW
authors: Ren
authors: Ming-ming
authors: MM
authors: Wang
authors: Ying-min
authors: YM
authors: Li
authors: Bin
authors: B
authors: Cong
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D018932:Chemokine CCL2 / Q000378:metabolism*; D024141:Collagen Type IV / Q000378:metabolism; D005786:Gene Expression Regulation; D005920:Glomerular Mesangium / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D050527:Mesangial Cells / Q000378:metabolism*; D051379:Mice; D015843:Serpins / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D024141:Collagen Type IV; C491938:Serpinb7 protein, mouse; D015843:Serpins; D018799:Intercellular Adhesion Molecule-1