title: C-terminal truncation of Vascular Endothelial Growth Factor mimetic helical peptide preserves structural and receptor binding properties.
abstract: Vascular Endothelial Growth Factor mimetic peptides have interesting applications in therapeutic angiogenesis. Recently, we described the proangiogenic properties of a 15 mer peptide designed on the N-terminal helix 17-25 of VEGF. The peptide was stabilized introducing well known peptide chemical tools among which N- and C-terminal capping sequence. Here, we show that the C-terminal sequence does not affect the structural and biological properties of the full-length peptide. In fact, a C-terminal truncated analog peptide resulted in a well folded and stable helix retaining the ability to bind to VEGF receptors. This study will allow to develop smaller peptidomimetic analogs able to modulate the VEGF-dependent angiogenesis.
authors: Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, 80134 Napoli, Italy.
authors: Barbara
authors: B
authors: Ziaco
authors: Donatella
authors: D
authors: Diana
authors: Domenica
authors: D
authors: Capasso
authors: Rosanna
authors: R
authors: Palumbo
authors: Veronica
authors: V
authors: Celentano
authors: Rossella
authors: R
authors: Di Stasi
authors: Roberto
authors: R
authors: Fattorusso
authors: Luca Domenico
authors: LD
authors: D'Andrea
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D006801:Humans; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology; D011485:Protein Binding; D017433:Protein Structure, Secondary; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry* / Q000494:pharmacology; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000737:chemistry*
chemical_list: D010455:Peptides; C531544:QK VEGF mimetic peptide; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1