title: A CCD-based reader combined quantum dots-labeled lateral flow strips for ultrasensitive quantitative detection of anti-HBs antibody.
abstract: Herein we reported a CCD-based reader combined quantum dots-labeled lateral flow strips for ultrasensitive quantitative detection of anti-HBs antibody. The CdTe quantum dots were prepared, then were used to label Hepatitis B Virus surface antigen, and then were fabricated into lateral flow strips. The as-prepared lateral flow strips were used to test different concentration of anti-HBV surface antibodies. The CCD-based reader was designed and fabricated, the quantitative analysis software was compiled, and resultant CCD-based reader system was used for quantitative analysis of examined anti-HBs antibodies on the strips. Results showed that the quantum dots-labeled lateral flow strips could detect the anti-HBs antibody with the limitation concentration of 200 pg/mL, the CCD-based reader system could detect anti-HBs antibody with the sensitivity of 2 pg/mL. In conclusion, the prepared CCD-based reader combined quantum dots-labeled lateral flow strips can be used for quantitative detection of anti-HBs antibody in sera with the sensitivity of 2 pg/mL, and has great potential in applications such as ultrasensitive detection of HBV antigens or antibodies, and other tumor biomarkers in near future.
authors: Department of Bio-Nano Science and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of Ministry of Education, National Key Laboratory of Micro/Nano Fabrication Technology, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
authors: Xueqing
authors: X
authors: Zhang
authors: Ding
authors: D
authors: Li
authors: Can
authors: C
authors: Wang
authors: Xiao
authors: X
authors: Zhi
authors: Chunlei
authors: C
authors: Zhang
authors: Kan
authors: K
authors: Wang
authors: Daxiang
authors: D
authors: Cui
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D006510:Hepatitis B Antibodies / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D010783:Photometry / Q000295:instrumentation*; D019095:Point-of-Care Systems; D045663:Quantum Dots; D011934:Reagent Strips; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*
chemical_list: D006510:Hepatitis B Antibodies; D011934:Reagent Strips