title: Proteoglycans from Boswellia serrata Roxb. and B. carteri Birdw. and identification of a proteolytic plant basic secretory protein.
abstract: Water-soluble high molecular weight compounds were isolated in yields of 21-22% from the oleogum of Boswellia serrata and B. carteri. Using anion exchange chromatography and gel permeation chromatography, different proteoglycans were purified and characterized, leading to four principally different groups: (i) Hyp-/Ser-rich extensins with O-glycosidic attached arabinan side chains; (ii) Modified extensins, with arabinogalactosylated side chains containing GlA and 4-O-Me-GlcA; (iii) Glycoproteins with N-glycosidic side chains containing higher amounts of Fuc, Man and GluNH(2,) featuring a 200 kD metalloproteinase that has been de novo sequenced and is described for the first time; (iv) Type II arabinogalactans-proteins. Significant differences between the gums from the two species were observed in the protein content (6% vs 22%), offering the possibility of a quick differentiation of gums from both species for analytical quality control. The data also offer an insight into the plant response towards wound-closing by the formation of extensin and AGP-containing gum.
authors: University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Münster, Germany.
authors: Andreas
authors: A
authors: Herrmann
authors: Simone
authors: S
authors: König
authors: Matthias
authors: M
authors: Lechtenberg
authors: Maria
authors: M
authors: Sehlbach
authors: Sergey Y
authors: SY
authors: Vakhrushev
authors: Jasna
authors: J
authors: Peter-Katalinic
authors: Andreas
authors: A
authors: Hensel
mesh_terms: D000595:Amino Acid Sequence; D029743:Boswellia / Q000737:chemistry* / Q000201:enzymology; D005685:Galactans; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification; D045726:Metalloproteases / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D053149:Plant Gums / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005685:Galactans; D006023:Glycoproteins; D053149:Plant Gums; D010940:Plant Proteins; D011509:Proteoglycans; C005488:extensin protein, plant; D045726:Metalloproteases; C005653:arabinogalactan