title: [Sulfatide-loaded CD1d tetramer to  detect  typeII  NKT cells in mice].
abstract: OBJECTIVE
  To create a method of detecting typeII  natural killer T (NKT) cells of mice.
  
  
  METHODS
  Biotinylated mouse CD1d monomers were mixed with sulfatide at a molar ratio of 1:3 (protein:lipid) and incubated at room temperature overnight,  and then 80 μg of streptavidin-PE was added into 200 μg of the CD1d-sulfatide mixture and incubated at room temperature for 4 h to get sulfatide/CD1d tetramer. Flow cytometry was used to detect the percentage of typeII  NKT cells in mononuclear cells (MNCs) of lung and spleen of normal mice,  as well as the percentage of typeII NKT cells in spleen MNCs of mice after stimulated with sulfatide.
  
  
  RESULTS
  In normal mice,  the percentage of typeII  NKT cells accounted for (0.875±0.096)% and (1.175±0.263)% in MNCs of spleen and lung;   the percentage in spleen MNCs after activated with sulfatide was (2.75±0.603)%,  which significantly increased as compared with that in normal mice (P<0.01).
  
  
  CONCLUSIONS
  Sulfatide-loaded CD1d tetramer is an effective method of detecting typeII  NKT cells in mice.
authors: Department of Respiratory Diseases, Zhongnan Hospital, Wuhan University, China. zgqxiao@126.com
authors: Gu-qin
authors: GQ
authors: Zhang
authors: Han-xiang
authors: HX
authors: Nie
authors: Jiong
authors: J
authors: Yang
authors: Hong-ying
authors: HY
authors: Yu
mesh_terms: D000818:Animals; D055610:Antigens, CD1d / Q000737:chemistry* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D055611:Natural Killer T-Cells / Q000276:immunology* / Q000378:metabolism; D055503:Protein Multimerization; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013433:Sulfoglycosphingolipids / Q000737:chemistry* / Q000276:immunology
chemical_list: D055610:Antigens, CD1d; D013433:Sulfoglycosphingolipids