title: HPMA copolymer-doxorubicin conjugates: The effects of molecular weight and architecture on biodistribution and in vivo activity.
abstract: The molecular weight and molecular architecture of soluble polymer drug carriers significantly influence the biodistribution and anti-tumour activities of their doxorubicin (DOX) conjugates in tumour-bearing mice. Biodistribution of N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer-DOX conjugates of linear and star architectures were compared in EL4 T-cell lymphoma-bearing mice. Biodistribution, including tumour accumulation, and anti-tumour activity of the conjugates strongly depended on conjugate molecular weight (MW), polydispersity, hydrodynamic radius (R(h)) and molecular architecture. With increasing MW, renal clearance decreased, and the conjugates displayed extended blood circulation and enhanced tumour accumulation. The linear conjugates with flexible polymer chains were eliminated by kidney clearance more quickly than the highly branched star conjugates with comparable MWs. Interestingly, the data suggested different mechanisms of renal filtration for star and linear conjugates. Only star conjugates with MWs below 50,000g.mo(-1) were removed by kidney filtration, while linear polymer conjugates with MWs near 70,000g.mol(-1), exceeding the generally accepted limit for renal elimination, were detected in the urine 36-96h after injection. Additionally, survival of tumour-bearing mice was strongly dependent on molecular weight and polymer conjugate architecture. Treatment of mice with the lower MW conjugate at a dose of 10mg DOX eq./kg resulted in 12% long-term surviving animals, while treatment with the corresponding star conjugate enabled 75% survival of animals.
authors: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic v.v.i., Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic.
authors: Tomáš
authors: T
authors: Etrych
authors: Vladimír
authors: V
authors: Subr
authors: Jiří
authors: J
authors: Strohalm
authors: Milada
authors: M
authors: Sírová
authors: Blanka
authors: B
authors: Ríhová
authors: Karel
authors: K
authors: Ulbrich
mesh_terms: D000178:Acrylamides / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000627:therapeutic use; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000737:chemistry* / Q000493:pharmacokinetics*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000627:therapeutic use; D004355:Drug Stability; D053208:Kaplan-Meier Estimate; D008223:Lymphoma / Q000188:drug therapy*; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D013329:Structure-Activity Relationship; D014018:Tissue Distribution
chemical_list: D000178:Acrylamides; D000903:Antibiotics, Antineoplastic; C482378:N-(2-hydroxypropyl)methacrylamide co-polymer-doxorubicin conjugate; D004317:Doxorubicin