title: Characterization of microsatellites in the mountain plant Armeria caespitosa (Plumbaginaceae) and transferability to congeners.
abstract: OBJECTIVE
  The focus of this study is to develop microsatellite markers in Armeria caespitosa, a narrow endemic of central Spain. Microsatellite loci are sought to clarify population structure and estimate gene flux among populations.
  
  
  RESULTS
  Enriched microsatellite genomic libraries were used for microsatellite isolation. Sixteen microsatellite loci were characterized, eight of which can be used for successful genotyping. Allele number ranged from two to seven per locus. Observed and expected heterozygosity ranged from 0.300 to 0.800 and from 0.296 to 0.733, respectively. Cross-amplification of seven and six loci was successful for A. maritima and A. cantabrica, respectively.
  
  
  CONCLUSIONS
  These microsatellites are suitable in the study of population genetics and gene flow among A. caespitosa populations. The information provided by these markers may be useful in the study of this plant's response to global warming.
authors: Departamento de Biología y Geología, Universidad Rey Juan Carlos, Calle Tulipán s/n, Móstoles, Madrid, Spain. alfredo.gar.fer@gmail.com
authors: Alfredo
authors: A
authors: García-Fernández
authors: Carlos
authors: C
authors: Lara-Romero
authors: José Gabriel
authors: JG
authors: Segarra-Moragues
authors: José María
authors: JM
authors: Iriondo
authors: Alex
authors: A
authors: Widmer
authors: Adrian
authors: A
authors: Escudero
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D005828:Genetics, Population / Q000379:methods; D060005:Genotyping Techniques; D018895:Microsatellite Repeats; D029606:Plumbaginaceae / Q000235:genetics*; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D018744:DNA, Plant