title: Separation and determination of trace environmental estrogen through molecularly imprinted solid phase extraction coupled to high performance liquid chromatography.
abstract: In recent years, there has been an increasing demand on the determination of trace environmental estrogen. In this study, a new 17β-estradiol molecularly imprinted polymer was successfully synthesized on the surface of activated silica gel. This prepared molecularly imprinted polymer was characterized, and found to exhibit good recognition, high selectivity, and a rapid adsorption-desorption dynamic toward 17β-estradiol. Using the molecularly imprinted polymer as a sorbent, an effective method of solid phase extraction coupled to high performance liquid chromatography for the separation and determination of 17β-estradiol was developed. Under optimal conditions, the detection limit (S/N = 3) of 17β-estradiol was 12.0 ng L(-1). The enrichment factor was 198, and the linear range of the calibration graph was 0.05-300 mg L(-1). Blank water samples spiked with 17β-estradiol at three different levels were analyzed by the developed method to afford recoveries ranging from 82.7% to 103.0%. Moreover, this presented method was applied to the quantitative detection of 17β-estradiol in a polluted water sample with a level of 0.024 mg L(-1).
authors: College of Food Science and Engineering, Shandong Agricultural University, Tai'an, China.
authors: Zhang
authors: Z
authors: Jing
authors: Xie L
authors: XL
authors: Chao
authors: Cong
authors: C
authors: Li
authors: Xu Z
authors: XZ
authors: Xiang
authors: Qiao X
authors: QX
authors: Guang
authors: Song J
authors: SJ
authors: Ming
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004967:Estrogens / Q000737:chemistry* / Q000302:isolation & purification*; D054802:Molecular Imprinting / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D004967:Estrogens