title: Two-dimensional ECD FT-ICR mass spectrometry of peptides and glycopeptides.
abstract: 2D FT-ICR MS allows the correlation between precursor and fragment ions by modulating ion cyclotron radii for fragmentation modes with radius-dependent efficiency in the ICR cell without the need for prior ion isolation. This technique has been successfully applied to ion-molecule reactions, Collision-induced dissociation and infrared multiphoton dissociation. In this study, we used electron capture dissociation for 2D FT-ICR MS for the first time, and we recorded two-dimensional mass spectra of peptides and a mixture of glycopeptides that showed fragments that are characteristic of ECD for each of the precursor ions in the sample. We compare the sequence coverage obtained with 2D ECD FT-ICR MS with the sequence coverage obtained with ECD MS/MS and compare the sensitivities of both techniques. We demonstrate how 2D ECD FT-ICR MS can be implemented to identify peptides and glycopeptides for proteomics analysis.
authors: Miniaturisation pour la Synthèse, l'Analyse & la Protéomique (MSAP), USR CNRS 3290, Université de Lille 1 Sciences et Technologies, 59655 Villeneuve d'Ascq Cedex, France.
authors: Maria A
authors: MA
authors: van Agthoven
authors: Lionel
authors: L
authors: Chiron
authors: Marie-Aude
authors: MA
authors: Coutouly
authors: Marc-André
authors: MA
authors: Delsuc
authors: Christian
authors: C
authors: Rolando
mesh_terms: D000595:Amino Acid Sequence; D017357:Cyclotrons; D005583:Fourier Analysis; D006020:Glycopeptides / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D040901:Proteomics; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006020:Glycopeptides; D007477:Ions; D010455:Peptides