title: A germline-competent embryonic stem cell line from NOD.Cg-Prkdc               (                 scid               )               Il2rg                         (                 tm1Wjl               )/SzJ (NSG) mice.
abstract: The NOD.Cg-Prkdc                 (                   scid                 )                 Il2rg                 (                   tm1Wjl                 )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular for engrafting human tumor cells and human hematopoietic stem cells. In the latter case, these cells give rise to many human hematopoetic lineages in their NSG hosts, resulting in recapitulation of many of the features of a human immune system. However, the immune system of these "humanized mice" (huMice) is not completely functional, in part because of a lack of expression of necessary human cytokines and HLA molecules by NSG host tissues. In order to facilitate the genetic modification of this strain in order to improve the huMouse model, we have created germline competent ES cells of this strain in which such modifications can be carried out.
authors: Department of Microbiology and Immunology, Thomas Jefferson University, 233 S. 10th St, Philadelphia, PA 19107, USA. clandel@transposagenbio.com
authors: Carlisle P
authors: CP
authors: Landel
authors: Jennifer
authors: J
authors: Dunlap
authors: John B
authors: JB
authors: Patton
authors: Tim
authors: T
authors: Manser
mesh_terms: D000818:Animals; D002460:Cell Line / Q000166:cytology* / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000276:immunology; D005854:Germ Cells / Q000166:cytology / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D053631:Interleukin Receptor Common gamma Subunit / Q000172:deficiency / Q000235:genetics / Q000276:immunology; D051379:Mice; D016513:Mice, SCID; D014183:Transplantation, Heterologous
chemical_list: D053631:Interleukin Receptor Common gamma Subunit