title: Probe droplet arrays generated in the capillary for microarray analysis.
abstract: Microarray technology is a useful tool for nucleic acid detection and has been widely used in biology and related research fields. However, the procedure is labor intensive and time consuming. Microfluidic chip-based microarrays save time with better performance, but the low spot density and probe number limit its applications. To develop high performance microarrays with high spot density within a microchannel, a method is reported here for preparing microarrays in a capillary by generating probe droplet arrays. The probes in droplets are immobilized onto the inner wall of the capillary to form a one-dimensional probe array, and then a sample solution is introduced to hybridize with the probe array. The effect of the capillary's inner diameter was evaluated to realize a high-density probe array. The processes of array generation and probe immobilization were studied to avoid possible cross contamination. The background from probe immobilization during the array generation and incubation was quantified to assure sensitivity. Multiple sample detection was also demonstrated within one capillary. The capillary based microarray assay had high spot density, easy fabrication, fast detection, high sensitivity and multiple sample capacity.
authors: Pen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen 361005, PR China.
authors: Xiangmeng
authors: X
authors: Qu
authors: Yuqiao
authors: Y
authors: Wang
authors: Zhan
authors: Z
authors: Shi
authors: Guangchun
authors: G
authors: Fu
authors: Xiang
authors: X
authors: Zeng
authors: Xin
authors: X
authors: Li
authors: Hong
authors: H
authors: Chen
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009691:Nucleic Acid Denaturation; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D015345:Oligonucleotide Probes; D004247:DNA