title: [Effect of Toll-like receptor 2 and Toll-like receptor 4 on expression of receptor activator of nuclear factor-kappaB ligand in human periodontal ligament fibroblasts induced by lipopolysaccharide].
abstract: OBJECTIVE
  The aim of this study was to survey the influence of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) repression to receptor activator of nuclear factor-kappaB ligand (RANKL) expression of human periodontal ligament fibroblasts (HPDLFs) under the stimulation of lipopolysaccharide (LPS).
  
  
  METHODS
  The level of RANKL in HPDLFs stimulated by 100 ng x mL(-1), 1 microg x mL(-1) and 10 microg x mL(-1) Escherichia coli (E. coli) LPS after 6, 12, 24 and 48 h was detected by enzyme linked immunosorbent assay (ELISA). The level of RANKL in HPDLFs stimulated by 1 microg x mL(-1) E. coli LPS after pretreatment with different titre anti-TLR2+anti-TLR4, anti-TLR2 and anti-TLR4 antibody were observed respectively.
  
  
  RESULTS
  RANKL was detected at 6 h after stimulation with LPS, and the levels of these cytokine were highest at 24 h, and then gradually decreased. The regularity of each LPS concentration was approximately similar. After pretreatment with anti-TLR2+anti-TLR4, anti-TLR2 and anti-TLR4 antibody, the level of RANKL was significantly decreased under the stimulation of 1 microg x mL(-1) LPS (P<0.05). In the three groups, the expression of RANKL was significantly different (P<0.05). The level of RANKL in anti-TLR2+anti-TLR4 antibody pretreatment group was the lowest, the level in anti-TLR4 antibody pretreatment group was higher, and the level in anti-TLR2 antibody pretreatment group was the highest.
  
  
  CONCLUSIONS
  TLR2 and TLR4 participate in the process of RANKL expres-in HPDLFs induced by LPS. Anti-TLR4 antibody has better inhibition effect to RANKL expression of HPDLFs stimulated by LPS than anti-TLR2.
authors: Dept. of Endodontics, Dalian Stomatological Hospital, Dalian 116021, China.
authors: Xin
authors: X
authors: Yu
authors: Yueqiu
authors: Y
authors: Wang
authors: Mingheng
authors: M
authors: Li
authors: Qin
authors: Q
authors: Su
authors: Haiping
authors: H
authors: Xu
authors: Lu
authors: L
authors: Xing
mesh_terms: D004926:Escherichia coli; D005347:Fibroblasts; D006801:Humans; D008070:Lipopolysaccharides; D010513:Periodontal Ligament; D053245:RANK Ligand; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4
chemical_list: D008070:Lipopolysaccharides; D053245:RANK Ligand; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4