title: Interleukin-8 and -10 gene polymorphisms in irritable bowel syndrome.
abstract: Irritable bowel syndrome (IBS) is one of the most common gastrointestinal diagnoses seen by primary care providers and gastroenterologists. Proinflammatory cytokines interleukin (IL)-6 and IL-8 have been found increased in IBS patients. Cytokine gene single nucleotide polymorphisms (SNPs) of IL-8 and IL-10 have not been assessed in Mexican IBS patients. DNA was extracted from peripheral blood leucocytes of 45 IBS unrelated patients and 137 controls. Allele, genotype, and haplotype frequencies were determined by analyzing SNPs of IL-8 and IL-10 genes. IL-8 + 396 G allele (P = 0.02), IL-8 + 396(G/G) and IL-8 + 781(C/T) genotypes (P < 0.001), IL-10 - 1082A allele and IL-10 - 1082(A/A) genotype (P < 0.001) were significantly increased in the IBS group. Haplotypes IL-8 ATCC (P = 0.03) and IL-10 ACC (P < 0.001) were associated with susceptibility to develop IBS. An association of certain polymorphisms of IL-8 and IL-10 in IBS patients compared to controls was demonstrated, suggesting a role of these cytokine SNPs in the pathophysiology of IBS.
authors: Dirección de Investigación, Hospital General "Dr. Manuel Gea González", Calzada de Tlalpan 4800, Colonia Sección XVI, 14080 Mexico, DF, Mexico.
authors: Mirza
authors: M
authors: Romero-Valdovinos
authors: Areli
authors: A
authors: Gudiño-Ramírez
authors: Jesús
authors: J
authors: Reyes-Gordillo
authors: Williams Arony
authors: WA
authors: Martínez-Flores
authors: María Elena
authors: ME
authors: Ramírez-Miranda
authors: Pablo
authors: P
authors: Maravilla
authors: Angélica
authors: A
authors: Olivo-Díaz
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D016209:Interleukin-8 / Q000235:genetics*; D043183:Irritable Bowel Syndrome / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D016209:Interleukin-8; D016753:Interleukin-10