title: Rheonix CARD(®) Technology: An Innovative and Fully Automated Molecular Diagnostic Device.
abstract: A versatile microfluidic platform for the evolving molecular diagnostics industry is described. It incorporates low cost Rheonix CARD(®) (Chemistry and Reagent Device) technology to analyze a variety of clinical specimens. A patented lamination process incorporates all pumps, valves, microchannels and reaction compartments into an inexpensive disposable plastic device. Once an untreated clinical specimen is introduced, all assay steps, including cell lysis, nucleic acid purification, multiplex PCR, and end-point analysis, are automatically performed. Three distinct CARD assays are described which utilize either a low density microarray for multiplex detection of amplicons or an integrated primer extension assay to detect single nucleotide polymorphisms of interest. The STI (Sexually Transmitted Infections) CARD(®) is able to simultaneously detect four sexually transmitted infectious agents (N. gonorrhoeae, C.trachomatis, T. pallidum and T. vaginalis). Human C33A cervical epithelial cells were spiked with different levels of genomic DNA from the four species of interest, singly or in combination, and applied to the CARD device. Using multiplex PCR amplification of the targets followed by microarray detection, the CARD device was able to correctly detect a minimum of 10 copies of each of the four pathogens. The HPV (Human Papillomavirus) CARD(®) was able to detect and distinguish 20 different clinically relevant HPV types using cloned HPV DNA. In addition, the HPV CARD could identify HPV types in vaginal specimens previously demonstrated to contain high or low risk HPV using a currently commercially available testing method. Finally, the detection of specific single nucleotide polymorphisms (SNP) associated with warfarin dosing sensitivity was achieved on the Warfarin Genotyping CARD(®) by analyzing human buccal swabs. Once multiplex PCR was completed, the SNPs were detected using a primer extension assay.
authors: RHEONIX Inc., 22 Thornwood Drive, Ithaca, NY 14850.
authors: Gwendolyn
authors: G
authors: Spizz
authors: Lincoln
authors: L
authors: Young
authors: Rubina
authors: R
authors: Yasmin
authors: Zongyuan
authors: Z
authors: Chen
authors: Travis
authors: T
authors: Lee
authors: Deborah
authors: D
authors: Mahoney
authors: Xun
authors: X
authors: Zhang
authors: Greg
authors: G
authors: Mouchka
authors: Benjamin
authors: B
authors: Thomas
authors: Whitney
authors: W
authors: Honey
authors: Todd
authors: T
authors: Roswech
authors: Cristina
authors: C
authors: McGuire
authors: Richard
authors: R
authors: Montagna
authors: Peng
authors: P
authors: Zhou