title: Epigenetic alterations in human pluripotent stem cells: a tale of two cultures.
abstract: Human somatic cells can be reprogrammed into induced pluripotent stem cells (hiPSCs) with wide lineage differentiation potential in culture. However, reprogramming and long-term culture can also induce abnormalities in these pluripotent cells. This minireview discusses recent studies that have identified changes in imprinted gene expression and erosion of X chromosome inactivation in female hiPSCs and how understanding the sources and consequences of epigenetic variability in hiPSCs will impact disease modeling and clinical application in the future.
authors: Wellcome Trust Centre for Stem Cell Research, Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK. aw512@cam.ac.uk
authors: Anton
authors: A
authors: Wutz
mesh_terms: D018929:Cell Culture Techniques; D065150:Cellular Reprogramming / Q000235:genetics; D044127:Epigenesis, Genetic; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000378:metabolism*; D049951:X Chromosome Inactivation