title: The effect of troglitazone on lipid accumulation and related gene expression in Hanwoo muscle satellite cell.
abstract: The current study was undertaken to determine the effect of the troglitazone (TGZ) on the expression of peroxisome proliferator-activating receptor (PPARγ), CCAAT/enhancer-binding protein, fatty acid binding protein 4, calpain 1 (CAPN1), and lipid accumulation in the myotube of Hanwoo muscle satellite cells. The satellite cells were treated with 5, 10, and 50 μM of TGZ for indicated time intervals. TGZ promoted the trans-differentiation with significant increase in glycerol accumulation. Polymerase chain reaction (PCR) and microarray results indicated that the TGZ treatment significantly increased the expression of adipogenic transcription factors. TGZ (10 and 50 μM) increased the CAPN1 gene expression 2.2- and 2.6-fold in real-time polymerase chain reaction analysis and 0.52- and 0.25-fold in microarray analysis, respectively, when compared with their respective controls. This result suggests that CAPN1 gene might be involved in the adipogenic differentiation programs. In addition, 13 genes were upregulated and 12 genes were downregulated in microarray analysis. Most of the up/downregulated genes were directly linked with adipogenesis.
authors: Department of Animal Sciences, Chonbuk National University, #664-1 Duckjin-dong, Jeonju, South Korea.
authors: You Bing
authors: YB
authors: Yang
authors: Muthuraman
authors: M
authors: Pandurangan
authors: Dawoon
authors: D
authors: Jeong
authors: Inho
authors: I
authors: Hwang
mesh_terms: D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics / Q000378:metabolism; D002154:Calpain / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D045162:Thiazolidinediones / Q000494:pharmacology*; D000077288:Troglitazone
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D022782:CCAAT-Enhancer-Binding Protein-beta; D002839:Chromans; D050556:Fatty Acid-Binding Proteins; D047495:PPAR gamma; D010975:Platelet Aggregation Inhibitors; D045162:Thiazolidinediones; D002154:Calpain; C484807:CAPN1 protein, Bos taurus; D000077288:Troglitazone