title: Tris(hydroxymethyl)aminomethane-functionalized agarose particles: parameters affecting the binding of bovine serum albumin.
abstract: A new protein adsorbent is introduced based on the coupling of the common buffer ion, tris(hydroxymethyl)aminomethane, to the agarose gel Sepharose HP from GE Healthcare Bio-Sciences AB, Uppsala, Sweden. The article describes the synthesis of the new adsorbent and the use of BSA as a model in a binding study. By optimization of the coupling procedure, a maximum ligand density of 63.5 μmol/mL gel could be obtained. Adsorption equilibria were investigated in the pH range 5.0-8.0 and at salt concentrations of 0-0.4 mol/L. Binding of BSA under different conditions indicated that both electrostatic interaction and hydrogen bonding were involved in the adsorption process where the former played a major role.
authors: Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, P R China.
authors: Bin
authors: B
authors: Zhang
authors: Ye
authors: Y
authors: Wang
authors: Min
authors: M
authors: Gao
authors: Ming
authors: M
authors: Gu
authors: Changhai
authors: C
authors: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel / Q000295:instrumentation* / Q000379:methods; D007700:Kinetics; D011485:Protein Binding; D012685:Sepharose / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification*; D014325:Tromethamine / Q000737:chemistry*
chemical_list: D014325:Tromethamine; D012710:Serum Albumin, Bovine; D012685:Sepharose