title: Specific targeting of the metallophosphoesterase YkuE to the bacillus cell wall requires the twin-arginine translocation system.
abstract: The twin-arginine translocation (Tat) pathway is dedicated to the transport of fully folded proteins across the cytoplasmic membranes of many bacteria and the chloroplast thylakoidal membrane. Accordingly, Tat-dependently translocated proteins are known to be delivered to the periplasm of Gram-negative bacteria, the growth medium of Gram-positive bacteria, and the thylakoid lumen. Here, we present the first example of a protein, YkuE of Bacillus subtilis, that is specifically targeted by the Tat pathway to the cell wall of a Gram-positive bacterium. The cell wall binding of YkuE is facilitated by electrostatic interactions. Interestingly, under particular conditions, YkuE can also be targeted to the cell wall in a Tat-independent manner. The biological function of YkuE was so far unknown. Our present studies show that YkuE is a metal-dependent phosphoesterase that preferentially binds manganese and zinc.
authors: Department of Medical Microbiology, University of Groningen and University Medical Center Groningen, Hanzeplein 1, P. O. Box 30001, 9700 RB Groningen, The Netherlands.
authors: Carmine G
authors: CG
authors: Monteferrante
authors: Marcus
authors: M
authors: Miethke
authors: René
authors: R
authors: van der Ploeg
authors: Corinna
authors: C
authors: Glasner
authors: Jan Maarten
authors: JM
authors: van Dijl
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000201:enzymology / Q000235:genetics; D008345:Manganese / Q000378:metabolism*; D008667:Metalloproteins / Q000235:genetics / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D015032:Zinc / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008667:Metalloproteins; D008345:Manganese; D010749:Phosphoprotein Phosphatases; D015032:Zinc