title: Overexpression of miR-92a correlates with tumor metastasis and poor prognosis in patients with colorectal cancer.
abstract: OBJECTIVE
  MicroRNAs regulate gene expression at the post-transcriptional level and play important roles in cancer development, progression, and metastasis. The aim of this study was to investigate the expression of miR-92a in colorectal cancer and the normal adjacent mucosa and its potential relevance to clinicopathological characteristics and patient survival.
  
  
  METHODS
  Surgical specimens of cancer tissue and adjacent normal mucosa were obtained from 82 patients with colorectal carcinomas. The relative expression levels of miR-92a mRNA in the cancer and the normal adjacent mucosa were measured by quantitative real-time reverse transcriptase polymerase chain reaction. We analyzed their correlation with tumor metastasis, clinicopathologic parameters, and clinical outcome.
  
  
  RESULTS
  The relative expression levels of miR-92a were significantly higher in colorectal cancer tissues than in the normal adjacent mucosa (p < 0.001), and a high expression of miR-92a correlated with advanced clinical stage (p = 0.025), lymph node metastases (p = 0.015), and distant metastases (p = 0.046). Kaplan-Meier analysis indicated that patients with high miR-92a expression had a poor overall survival (p = 0.001). Moreover, multivariate analysis showed that increased expression of miR-92a was an independent predictor of overall survival.
  
  
  CONCLUSIONS
  This study revealed that miR-92a overexpression was correlated with specific colorectal cancer biopathologic features, such as TNM stage, lymph node and distant metastases, and poor survival of the patients, indicating that miR-92a may serve as a molecular prognostic marker for colorectal cancer and disease progression.
authors: The First Department of General Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
authors: Tong
authors: T
authors: Zhou
authors: Guangjun
authors: G
authors: Zhang
authors: Zuoliang
authors: Z
authors: Liu
authors: Shusen
authors: S
authors: Xia
authors: Hongpeng
authors: H
authors: Tian
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C541874:MIRN92 microRNA, human; D035683:MicroRNAs