title: Prolonged dietary selenium deficiency or excess does not globally affect selenoprotein gene expression and/or protein production in various tissues of pigs.
abstract: We previously determined the effects of dietary selenium (Se) deficiency or excess on mRNA abundance of 12 selenoprotein genes in pig tissues. In this study, we determined the effect of dietary Se on mRNA levels of the remaining porcine selenoprotein genes along with protein production of 4 selenoproteins (Gpx1, Sepp1, Selh, and Sels) and body glucose homeostasis. Weanling male pigs (n = 24) were fed a Se-deficient (<0.02 mg Se/kg), basal diet supplemented with 0, 0.3, or 3.0 mg Se/kg as Se-enriched yeast (Angel Yeast) for 16 wk. Although mRNA abundance of the 13 selenoproteins in 10 tissues responded to dietary Se in 3 patterns, there was no common regulation for any given gene across all tissues or for any given tissue across all genes. Dietary Se affected (P < 0.05) 2, 3, 3, 5, 6, 7, 7, and 8 selenoprotein genes in muscle, hypothalamus, liver, kidney, heart, spleen, thyroid, and pituitary, respectively. Protein abundance of Gpx1, Sepp1, Selh, and Sels in 6 tissues was regulated (P < 0.05) by dietary Se concentrations in 3 ways. Compared with those fed 0.3 mg Se/kg, pigs fed 3.0 mg Se/kg became hyperinsulinemic (P < 0.05) and had lower (P < 0.05) tissue levels of serine/threonine protein kinase. In conclusion, dietary Se exerted no global regulation of gene transcripts or protein levels of individual selenoproteins across porcine tissues. Pigs may be a good model for studying mechanisms related to the potential prodiabetic risk of high-Se intake in humans.
authors: International Center of Future Agriculture for Human Health, Sichuan Agricultural University, Chengdu, China.
authors: Yan
authors: Y
authors: Liu
authors: Hua
authors: H
authors: Zhao
authors: Qiaoshan
authors: Q
authors: Zhang
authors: Jiayong
authors: J
authors: Tang
authors: Ke
authors: K
authors: Li
authors: Xin-Jie
authors: XJ
authors: Xia
authors: Kang-Ning
authors: KN
authors: Wang
authors: Kui
authors: K
authors: Li
authors: Xin Gen
authors: XG
authors: Lei
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D007031:Hypothalamus / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012643:Selenium / Q000008:administration & dosage* / Q000172:deficiency*; D051140:Selenoproteins / Q000235:genetics / Q000378:metabolism*; D013552:Swine / Q000097:blood / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D051140:Selenoproteins; D012643:Selenium