title: Nematicidal activity of 2-thiophenecarboxaldehyde and methylisothiocyanate from caper (Capparis spinosa) against Meloidogyne incognita.
abstract: New pesticides based on plant extracts have recently gained interest in the development of nontoxic crop protection chemicals. Numerous research studies are focused on the isolation and identification of new active compounds derived from plants. In this manuscript we report about the use of the Mediterranean species Capparis spinosa as a potent natural nematicidal agent against the root knot nematodes Meloidogyne incognita. Leaves, stems, and caper buds of Capparis spinosa were used to obtain their methanol extracts (LME, SME, BME) that were successively in vitro tested against second stage nematode juveniles (J2). In terms of paralysis induction, the methanol extract of the stem part (SME) was found more effective against M. incognita and then the caper methanol buds and leaves extracts. The chemical composition analysis of the extracts carried out by GC/MS and LC/MS techniques showed that methylisothiocyanate was the main compound of SME. The EC50 for SME after 3 days of immersion was 215 ± 36 mg/L. The constituent components of SME such as 2-thiophenecarboxaldehyde and methylisothiocyanate were successively in vitro tested for their nematicidal activity against J2. Both compounds induced paralysis on root knot nematodes ranking first (EC50 = 7.9 ± 1.6, and 14.1 ± 1.9 mg/L respectively) for M. incognita. Moreover, 2-thiophenecarboxaldehyde showed a strong fumigant activity.
authors: Department of Life and Environmental Science and ‡Dipartimento di Scienze Biomediche, Università degli Studi di Cagliari , Via Ospedale 72, 09124 Cagliari, Italy.
authors: Pierluigi
authors: P
authors: Caboni
authors: Giorgia
authors: G
authors: Sarais
authors: Nadhem
authors: N
authors: Aissani
authors: Graziella
authors: G
authors: Tocco
authors: Nicola
authors: N
authors: Sasanelli
authors: Barbara
authors: B
authors: Liori
authors: Annarosa
authors: A
authors: Carta
authors: Alberto
authors: A
authors: Angioni
mesh_terms: D000447:Aldehydes / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000969:Antinematodal Agents / Q000737:chemistry / Q000494:pharmacology*; D031484:Capparis / Q000737:chemistry*; D002853:Chromatography, Liquid; D008401:Gas Chromatography-Mass Spectrometry; D017879:Isothiocyanates / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D013876:Thiophenes / Q000737:chemistry / Q000494:pharmacology*; D014431:Tylenchoidea / Q000187:drug effects*
chemical_list: C508885:2-thiophene carboxaldehyde; D000447:Aldehydes; D000969:Antinematodal Agents; D017879:Isothiocyanates; D010936:Plant Extracts; D013876:Thiophenes; C005227:methyl isothiocyanate