title: Nitric oxide and hydrogen peroxide alleviate drought stress in marigold explants and promote its adventitious root development.
abstract: Drought stress is one of the most important environmental factors that regulates plant growth and development. In this study, we examined the effects of nitric oxide (NO) and hydrogen peroxide (H(2)O(2)) on adventitious rooting in marigold (Tagetes erecta L.) under drought stress. The results showed that the promoting effect of NO or H(2)O(2) on rooting under drought stress was dose-dependent, with a maximal biological response at 10 μM NO donor sodium nitroprusside (SNP) or 600 μM H(2)O(2). Results also indicated that endogenous NO and H(2)O(2) may play crucial roles in rooting under drought conditions, and H(2)O(2) may be involved in rooting promoted by NO under drought stress. NO or H(2)O(2) treatment attenuated the destruction of mesophyll cells ultrastructure by drought stress. Similarly, NO or H(2)O(2) increased leaf chlorophyll content, chlorophyll fluorescence parameters (Fv/Fm, ΦPS II and qP), and hypocotyls soluble carbohydrate and protein content, while decreasing starch content. Results suggest that the protection of mesophyll cells ultrastructure by NO or H(2)O(2) under drought conditions improves the photosynthetic performance of leaves and alleviates the negative effects of drought on carbohydrate and nitrogen accumulation in explants, thereby adventitious rooting being promoted.
authors: Gansu Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, No. 1 Yinmen Village, Anning District, Lanzhou 730070, China. liaowb@gsau.edu.cn
authors: Wei-Biao
authors: WB
authors: Liao
authors: Gao-Bao
authors: GB
authors: Huang
authors: Ji-Hua
authors: JH
authors: Yu
authors: Mei-Ling
authors: ML
authors: Zhang
mesh_terms: D000222:Adaptation, Physiological; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002734:Chlorophyll / Q000502:physiology; D055864:Droughts; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D018546:Hypocotyl / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D009584:Nitrogen / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development*; D013312:Stress, Physiological; D031686:Tagetes / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D014867:Water / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D014867:Water; D002734:Chlorophyll; D009599:Nitroprusside; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D009584:Nitrogen