title: Endocytic adaptor protein intersectin 1 forms a complex with microtubule stabilizer STOP in neurons.
abstract: Intersectin 1 (ITSN1) is a multidomain adaptor protein that functions in clathrin-mediated endocytosis and signal transduction. This protein is highly abundant in neurons and is implicated in Down syndrome, Alzheimer's disease and, possibly, other neurodegenerative disorders. Here we used an in vitro binding assay combined with MALDI-TOF mass spectrometry to identify novel binding partners of ITSN1. We found that the neuron-specific isoform of the stable tubule-only polypeptide (STOP) interacts with SH3A domain of ITSN1. STOP and ITSN1 were shown to form a complex in vivo and to partially co-localize in rat primary hippocampal neurons. As STOP is a microtubule-stabilizing protein that is required for several forms of synaptic plasticity in the hippocampus, identification of this interaction raises the possibility of ITSN1 participation in this process.
authors: State Key Laboratory on Molecular and Cellular Biology, Institute of Molecular Biology and Genetics, 150 Zabolotnogo Street, Kyiv 03680, Ukraine.
authors: Dmytro
authors: D
authors: Morderer
authors: Oleksii
authors: O
authors: Nikolaienko
authors: Inessa
authors: I
authors: Skrypkina
authors: Volodymyr
authors: V
authors: Cherkas
authors: Liudmyla
authors: L
authors: Tsyba
authors: Pavel
authors: P
authors: Belan
authors: Alla
authors: A
authors: Rynditch
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C113727:Map6 protein, rat; D008869:Microtubule-Associated Proteins; D020033:Protein Isoforms; C115919:intersectin 1