title: Construction of logic gates with the fluorene-based small molecule/DNA probes.
abstract: Fluorene-based small molecules (FSMs) have optical properties and can interact with DNA. In this paper, the integrated "INH" and "AND" gates operating in parallel are developed with the fluorene-based small molecule (FSM)/DNA probe. They are activated by taking advantage of the two-step fluorescence resonance energy transfer (FRET) process and the sequence-recognition mechanism of DNA. Then, a "NOT" gate is obtained with a molecular beacon-like probe (FSM-MB) by using the FSM as the fluorophore. Moreover, the "NOT" gate based on the FSM-MB probe can be used as a biosensor and has potential applications in label-free detection of target molecules.
authors: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
authors: Jing
authors: J
authors: Guo
authors: Ting
authors: T
authors: Wang
authors: Renqiang
authors: R
authors: Yang
mesh_terms: D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D005449:Fluorenes / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D015394:Molecular Structure
chemical_list: D015342:DNA Probes; D005449:Fluorenes; C041509:fluorene; D004247:DNA