title: LAP2 is widely overexpressed in diverse digestive tract cancers and regulates motility of cancer cells.
abstract: BACKGROUND
  Lamina-associated polypeptides 2 (LAP2) is a nuclear protein that connects the nuclear lamina with chromatin. Although its critical roles in genetic disorders and hematopoietic malignancies have been described, its expression and roles in digestive tract cancers have been poorly characterized.
  
  
  METHODS
  To examine the expression of LAP2 in patient tissues, we performed immunohistochemistry and real-time PCR. To examine motility of cancer cells, we employed Boyden chamber, wound healing and Matrigel invasion assays. To reveal its roles in metastasis in vivo, we used a liver metastasis xenograft model. To investigate the underlying mechanism, a cDNA microarray was conducted.
  
  
  RESULTS
  Immunohistochemistry in patient tissues showed widespread expression of LAP2 in diverse digestive tract cancers including stomach, pancreas, liver, and bile duct cancers. Real-time PCR confirmed that LAP2β is over-expressed in gastric cancer tissues. Knockdown of LAP2β did not affect proliferation of most digestive tract cancer cells except pancreatic cancer cells. However, knockdown of LAP2β decreased motility of all tested cancer cells. Moreover, overexpression of LAP2β increased motility of gastric and pancreatic cancer cells. In the liver metastasis xenograft model, LAP2β increased metastatic efficacy of gastric cancer cells and mortality in tested mice. cDNA microarrays showed the possibility that myristoylated alanine-rich C kinase substrate (MARCKS) and interleukin6 (IL6) may mediate LAP2β-regulated motility of cancer cells.
  
  
  CONCLUSIONS
  From the above results, we conclude that LAP2 is widely overexpressed in diverse digestive tract cancers and LAP2β regulates motility of cancer cells and suggest that LAP2β may have utility for diagnostics and therapeutics in digestive tract cancers.
authors: Department of Anatomy, School of Medicine, Pusan National University, Pusan, Republic of Korea.
authors: Hyun-Jung
authors: HJ
authors: Kim
authors: Sun-Hwi
authors: SH
authors: Hwang
authors: Myoung-Eun
authors: ME
authors: Han
authors: Sungmin
authors: S
authors: Baek
authors: Hey-Eun
authors: HE
authors: Sim
authors: Sik
authors: S
authors: Yoon
authors: Sun-Yong
authors: SY
authors: Baek
authors: Bong-Seon
authors: BS
authors: Kim
authors: Jeong-Hwan
authors: JH
authors: Kim
authors: Seon-Young
authors: SY
authors: Kim
authors: Sae-Ock
authors: SO
authors: Oh
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005770:Gastrointestinal Neoplasms / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004268:DNA-Binding Proteins; D008565:Membrane Proteins; C093413:lamina-associated polypeptide 2