title: Detection of Acinetobacter baumannii in human head and body lice from Ethiopia and identification of new genotypes.
abstract: BACKGROUND
  Acinetobacter baumannii has previously been detected and genotyped in human body lice. The objectives of this study were to determine the presence of this bacterium in head and body lice collected from healthy individuals in Ethiopia by molecular methods and to characterize the genotype.
  
  
  METHODS
  Human lice from locations at different altitudes in Ethiopia were screened for the presence of Acinetobacter sp by targeting the rpoB gene. Acinetobacter baumannii was detected and genotyped using recA PCR amplification.
  
  
  RESULTS
  A total of 115 head and 109 body lice were collected from 134 healthy individuals. Acinetobacter sp were found in 54 head (47%) and 77 body (71%) lice. The recA gene was sequenced for 60 of the Acinetobacter sp and 67% were positive for A. baumannii; genotype 1 was retrieved the most frequently.
  
  
  CONCLUSIONS
  Our study is the first to show the presence of A. baumannii in human body lice, and also in head lice, in Ethiopia.
authors: URMITE, UMR IRD 198/CNRS 6236, Faculté de Médecine, 27 Bd Jean Moulin, 13385 Marseille cedex 05, France.
authors: Marie
authors: M
authors: Kempf
authors: Alemseged
authors: A
authors: Abdissa
authors: Georges
authors: G
authors: Diatta
authors: Jean-François
authors: JF
authors: Trape
authors: Emmanouil
authors: E
authors: Angelakis
authors: Oleg
authors: O
authors: Mediannikov
authors: Bernard
authors: B
authors: La Scola
authors: Didier
authors: D
authors: Raoult
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology* / Q000469:parasitology*; D040981:Acinetobacter baumannii / Q000235:genetics / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D005002:Ethiopia / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006801:Humans; D010373:Lice Infestations / Q000453:epidemiology / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D010374:Pediculus / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011938:Rec A Recombinases / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C089760:RecA protein, Acinetobacter calcoaceticus; D011938:Rec A Recombinases