title: Crystallization and preliminary X-ray crystallographic analysis of Pz peptidase B from Geobacillus collagenovorans MO-1.
abstract: Pz peptidase B is an intracellular M3 metallopeptidase that is found together with Pz peptidase A in the thermophile Geobacillus collagenovorans MO-1 and recognizes collagen-specific tripeptide units (-Gly-Pro-X-). These peptidases have low homology in their primary structures; however, their cleavage patterns towards peptide substrates are similar. In this work, Pz peptidase B was crystallized using the counter-diffusion method. Data were collected to a resolution of 1.6 Å at 100 K from a crystal obtained in the Japanese Experiment Module (JEM; also known as `Kibo') at the International Space Station (ISS). The crystal belonged to the trigonal space group P3(1)21, with unit-cell parameters a = b = 87.64, c = 210.5 Å. A complete data set was also obtained from crystals of selenomethionine-substituted protein.
authors: Department of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo, Kobe 650-8530, Japan.
authors: Hiroaki
authors: H
authors: Nakano
authors: Allin
authors: A
authors: Hosokawa
authors: Ryuji
authors: R
authors: Tagawa
authors: Koji
authors: K
authors: Inaka
authors: Kazunori
authors: K
authors: Ohta
authors: Toru
authors: T
authors: Nakatsu
authors: Hiroaki
authors: H
authors: Kato
authors: Kunihiko
authors: K
authors: Watanabe
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D056506:Geobacillus / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000737:chemistry*
chemical_list: D010447:Peptide Hydrolases