title: [New opportunities of renal diseases diagnostics using gas chromatography].
abstract: During the last decade the amount of patients suffering from chronic kidney disease (CKD) has increased. The physicians' efforts are focused on early CKD diagnosis and reduction of the end-stage renal diseases incidence. The breath test seems to be a promising diagnostic device offering early noninvasive diseases detection. The aim of presented study was the determination of breath composition in case of persons suffering from CKD. Breath samples were collected from 14 patients and 7 healthy volunteers. Exhaled air samples were analyzed by gas chromatography equipped with mass spectrometer (GC-MS). Samples were enriched using solid phase microextraction (SPME). Trimethylamine (TMA), mentioned in literature as potential marker of chronic kidney diseases, was detected in case of 11 patients. Among breath components were detected: sulfur compounds: dimethyl sulfide (was detected in exhaled air of patients and control group), carbon disulfide (was detected in case of 4 healthy and in case of all patients) and also potential markers of oxidative stress: propane, butane, pentane, 2-methylpentane, hexane. Acetone and isoprene occurred in exhaled air of all studied persons. The considerable increase of acetone concentration in comparison to control group was observed in case of patients with diagnosed diabetes. The application of gas chromatography with mass spectrometer and appropriate enrichment of samples allows to define the breath profile characteristic for chosen the unit of disease. Typical compounds--biomarkers can be useful for early diagnostics.
authors: Zakład Fizykochemii Ekosystemów IFJ PAN w Krakowie. beata.grabowska@ifj.edu.pl
authors: Beata
authors: B
authors: Grabowska-Polanowska
authors: Jacek
authors: J
authors: Faber
authors: Monika
authors: M
authors: Skowron
authors: Przemysław
authors: P
authors: Miarka
authors: Agata
authors: A
authors: Pietrzycka
authors: Paweł
authors: P
authors: Zagrodzki
authors: Ireneusz
authors: I
authors: Sliwka
authors: Władysław
authors: W
authors: Sutowicz
authors: Marek
authors: M
authors: Stepniewski
mesh_terms: D000096:Acetone / Q000032:analysis; D015415:Biomarkers / Q000032:analysis*; D001944:Breath Tests / Q000379:methods*; D002246:Carbon Disulfide / Q000032:analysis; D002908:Chronic Disease; D003928:Diabetic Nephropathies / Q000175:diagnosis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007674:Kidney Diseases / Q000175:diagnosis* / Q000209:etiology; D008297:Male; D008744:Methylamines / Q000032:analysis; D008875:Middle Aged; D013440:Sulfides / Q000032:analysis
chemical_list: D015415:Biomarkers; D008744:Methylamines; D013440:Sulfides; D000096:Acetone; C023336:trimethylamine; C004784:dimethyl sulfide; D002246:Carbon Disulfide