title: Expression of gene associated with retinoid-interferon-induced mortality-19 in preimplantation embryo of mice.
abstract: OBJECTIVE
  To study the expression of gene associated with retinoid-interferon-induced mortality-19(GRIM-19) in preimplantation embryo of mice and explore its role in embryonic development.
  
  
  METHODS
  The protein and mRNA expressions of GRIM-19 in 2-cell, 4-cell, 8-cell, morula, and blastocyst phases of mice preimplantation embryo were detected by Western blot analysis and Real-time polymerase chain reaction (PCR).
  
  
  RESULTS
  GRIM-19 was continuously expressed in every stage of preimplantation embryo of mice. Western blot analysis and Real-time PCR demonstrated a gradual increase of GRIM-19 expression from 2-cell, which reached a peak in 8-cell phase and then decreased progressively.
  
  
  CONCLUSIONS
  The expression of GRIM-19 in mouse preimplantation embryos changes as at different developmental phases. GRIM-19 may play an important role during embryonic development.
authors: Infertility Center, Qilu Hospital, Shandong University, Jinan 250012, China.
authors: Wen-juan
authors: WJ
authors: Cui
authors: Lan
authors: L
authors: Chao
authors: Xiao-hui
authors: XH
authors: Deng
authors: Yan-jun
authors: YJ
authors: Shen
authors: Fang
authors: F
authors: Yang
authors: Wen-juan
authors: WJ
authors: Feng
authors: Jing
authors: J
authors: Xu
authors: Hong-lei
authors: HL
authors: Chen
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D007372:Interferons / Q000494:pharmacology*; D051379:Mice; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D012333:RNA, Messenger; D014212:Tretinoin; D007372:Interferons; D009247:NADH, NADPH Oxidoreductases; C486684:Grim19 protein, mouse