title: Ultrafast, large-field multiphoton microscopy based on an acousto-optic deflector and a spatial light modulator.
abstract: We present an ultrafast, large-field multiphoton excitation fluorescence microscope with high lateral and axial resolutions based on a two-dimensional (2-D) acousto-optical deflector (AOD) scanner and spatial light modulator (SLM). When a phase-only SLM is used to shape the near-infrared light from a mode-locked titanium:sapphire laser into a multifocus array including the 0-order beam, a 136 μm × 136 μm field of view is achieved with a 60× objective using a 2-D AOD scanner without any mechanical scan element. The two-photon fluorescence image of a neuronal network that was obtained using this system demonstrates that our microscopy permits observation of dynamic biological events in a large field with high-temporal and -spatial resolution.
authors: College of Optoelectronics Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
authors: Yonghong
authors: Y
authors: Shao
authors: Wan
authors: W
authors: Qin
authors: Honghai
authors: H
authors: Liu
authors: Junle
authors: J
authors: Qu
authors: Xiang
authors: X
authors: Peng
authors: Hanben
authors: H
authors: Niu
authors: Bruce Z
authors: BZ
authors: Gao
mesh_terms: D000162:Acoustics; D000818:Animals; D008027:Light; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D057054:Molecular Imaging; D030007:Nerium / Q000166:cytology; D009474:Neurons / Q000166:cytology; D055620:Optical Phenomena; D011058:Pollen / Q000166:cytology; D013997:Time Factors