title: Selective recognition of DNA from olive leaves and olive oil by PNA and modified-PNA microarrays.
abstract: PNA probes for the specific detection of DNA from olive oil samples by microarray technology were developed. The presence of as low as 5% refined hazelnut (Corylus avellana) oil in extra-virgin olive oil (Olea europaea L.) could be detected by using a PNA microarray. A set of two single nucleotide polymorphisms (SNPs) from the Actin gene of Olive was chosen as a model for evaluating the ability of PNA probes for discriminating olive cultivars. Both unmodified and C2-modified PNAs bearing an arginine side-chain were used, the latter showing higher sequence specificity. DNA extracted from leaves of three different cultivars (Ogliarola leccese, Canino and Frantoio) could be easily discriminated using a microarray with unmodified PNA probes, whereas discrimination of DNA from oil samples was more challenging, and could be obtained only by using chiral PNA probes.
authors: Dipartimento di Chimica Organica e Industriale, Università di Parma, Parma, Italy.
authors: Stefano
authors: S
authors: Rossi
authors: Alessandro
authors: A
authors: Calabretta
authors: Tullia
authors: T
authors: Tedeschi
authors: Stefano
authors: S
authors: Sforza
authors: Sergio
authors: S
authors: Arcioni
authors: Luciana
authors: L
authors: Baldoni
authors: Roberto
authors: R
authors: Corradini
authors: Rosangela
authors: R
authors: Marchelli
mesh_terms: D031211:Corylus / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics*; D005506:Food Contamination / Q000032:analysis; D031658:Olea / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D000069463:Olive Oil; D020135:Peptide Nucleic Acids / Q000032:analysis* / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics*; D010938:Plant Oils / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013379:Substrate Specificity
chemical_list: D018744:DNA, Plant; D000069463:Olive Oil; D020135:Peptide Nucleic Acids; D010938:Plant Oils