title: Inhibitory effect of Salicornia europaea on the marine alga Skeletonema costatum.
abstract: Exploiting the negative biochemical interference between plants and algal species has been suggested as a method to control harmful algal blooms. In this work, we investigated the inhibitory effect of the salt marsh halophyte Salicornia europaea against the marine alga Skeletonema costatum. S. europaea suppressed the growth of S. costatum in a nutrient-sufficient co-culture system, indicating that the inhibition of algal growth was because of the phytotoxic effect of S. europaea, rather than nutrient competition. We tested aqueous and organic extracts from S. europaea roots against S. costatum. The organic extracts inhibited growth and affected the cell size and chlorophyll a content of S. costatum in a dose-dependent manner. Among the three tested organic extracts, the methanol extract had the greatest effects on S. costatum, followed by butanol extract, and then the chloroform extract. Two flavonoids, rutin and quercetin-3-β-D-glucoside, were identified in the methanol extract by high performance liquid chromatography. The concentration of rutin was much higher than that of quercetin-3-β-D-glucoside. In an algal bioassay, rutin inhibited the growth of S. costatum and the inhibitory effect increased with increasing rutin concentration and with decreasing initial algal density. Therefore, we concluded that S. europaea negatively affects the growth of S. costatum, and that rutin, a metabolite of S. europaea, may play a role in this inhibitory effect.
authors: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors: Dan
authors: D
authors: Jiang
authors: Lingfeng
authors: L
authors: Huang
authors: Yongqing
authors: Y
authors: Lin
authors: Lingling
authors: L
authors: Nie
authors: Sulian
authors: S
authors: Lv
authors: Tingyun
authors: T
authors: Kuang
authors: Yinxin
authors: Y
authors: Li
mesh_terms: D019663:Chenopodiaceae / Q000737:chemistry* / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid; D017377:Diatoms / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D017753:Ecosystem; D008386:Marine Biology; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry* / Q000254:growth & development; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012431:Rutin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012623:Seawater / Q000382:microbiology
chemical_list: D010936:Plant Extracts; C016527:isoquercitrin; D012431:Rutin; D011794:Quercetin; D000432:Methanol