title: Effect of zinc(II) ions on the expression of pro- and anti-apoptotic             factors in high-grade prostate carcinoma cells.
abstract: Several typical characteristics of prostate tissue have been identified             including the ability to accumulate zinc(II). However, this feature of prostate             cells is lost during carcinogenesis and, thus, prostate cells are unable to accumulate             zinc(II) ions in high levels. Therefore, we can expect that zinc(II) ions can             significantly contribute to the progression of tumour disease and to the ability             of prostate cell lines to metastasize. In this study, we aimed our attention on             determining the expression of Bcl-2, c-Fos, c-Jun, Ki-67, NF-κB and p53 genes             in two prostate cell lines, as the 22Rv1 cell line, a model of aggressive partially             androgen-sensitive prostate cancer and the PNT1A cell line, a normal prostate             cell line model. Moreover, we were interested in the mechanisms through which             exposure of these cell lines to zinc(II) ions could influence expression of the             above-mentioned genes. We found that zinc(II) ions caused elevated expression             of Ki-67, a marker of proliferation, extremely low expression of p53, high expression             of Bcl-2 and no changes in the expression of p53. Our experimental data show different             effect of zinc(II) ions on expression of the above-mentioned regulatory genes,             which may give us more information on their impact on cancer development and progression             with possible using for cancer therapy.
authors: Department of Pathological Physiology, Faculty of Medicine, Masaryk University, CZ-625 00 Brno, Czech Republic.
authors: Marketa
authors: M
authors: Sztalmachova
authors: Marian
authors: M
authors: Hlavna
authors: Jaromir
authors: J
authors: Gumulec
authors: Monika
authors: M
authors: Holubova
authors: Petr
authors: P
authors: Babula
authors: Jan
authors: J
authors: Balvan
authors: Jiri
authors: J
authors: Sochor
authors: Veronika
authors: V
authors: Tanhauserova
authors: Martina
authors: M
authors: Raudenska
authors: Sona
authors: S
authors: Krizkova
authors: Vojtech
authors: V
authors: Adam
authors: Tomas
authors: T
authors: Eckschlager
authors: Rene
authors: R
authors: Kizek
authors: Michal
authors: M
authors: Masarik
mesh_terms: D000328:Adult; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D002413:Cations, Divalent; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011471:Prostatic Neoplasms; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D019287:Zinc Sulfate / Q000494:pharmacology*
chemical_list: D051017:Apoptosis Regulatory Proteins; D002413:Cations, Divalent; D019394:Ki-67 Antigen; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D016760:Proto-Oncogene Proteins c-fos; D013438:Sulfhydryl Compounds; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D019287:Zinc Sulfate