title: [Vitrification of oocytes for in vitro fertilization and embryo transfer].
abstract: OBJECTIVE
  To explore the feasibility, indication and method of oocyte vitrification during the IVF - ET procedure, so as to increase the utilization of oocytes and reduce oocyte waste.
  
  
  METHODS
  This study included the patients whose husbands failed to provide sperm samples at the time of oocyte pickup or from whom more than 25 oocytes were obtained. With the patients' consent, some of their oocytes were subjected to cryopreservation by vitrification, and used for IVF - ET after thawed.
  
  
  RESULTS
  Totally, 53 oocytes from 7 patients were thawed, and 44 (83.02%) survived, of which 41 M II oocytes were subjected to ICSI and 32 (72.73%) were fertilized. Thirty embryos were formed, with a cleavage rate of 93.75%. Sixteen embryos were transferred in 9 cycles, with achievement of 2 clinical pregnancies and delivery of 3 healthy babies. The implantation rate was 18.75% and the live birth rate 22.22%. Seven of the embryos were still cryopreserved.
  
  
  CONCLUSIONS
  Cryopreservation of oocytes by vitrification effects satisfactory rates of survival and fertilization, and that of surplus oocytes can increase oocyte utilization and adds to the alternatives for IVF - ET.
authors: Center of Reproductive Medicine, The First Maternity and Infant Hospital Affiliated to Tongji University School of Medicine, Shanghai 200040, China. tengxiaoming@hotmail.com
authors: Xiao-Ming
authors: XM
authors: Teng
authors: Kun-Ming
authors: KM
authors: Li
authors: Yu
authors: Y
authors: Wang
authors: Jia-Ping
authors: JP
authors: Pan
authors: Ping
authors: P
authors: Yin
authors: Shan-Shan
authors: SS
authors: Liang
authors: Jing-Ling
authors: JL
authors: Ruan
authors: Zhi-Qin
authors: ZQ
authors: Chen
mesh_terms: D000328:Adult; D015925:Cryopreservation / Q000379:methods*; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D009865:Oocytes; D011247:Pregnancy; D018873:Pregnancy Rate; D058989:Vitrification