title: Resveratrol and some glucosyl, glucosylacyl, and glucuronide derivatives reduce Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes Scott A adhesion to colonic epithelial cell lines.
abstract: The efficacy of resveratrol and some glucosyl, glucosylacyl, and glucuronide derivatives in inhibiting the adhesion of Salmonella Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes Scott A to Caco-2 and HT-29 colonic cells was investigated. The three bacteria strains were capable of adhering to both colonic epithelial cell lines, which responded by producing the pro-inflammatory interleukin 8 (IL-8). Adhesion inhibition of E. coli O157:H7 and S. Typhimurium to colonic cells was ≥60 and ≥40%, respectively, when resveratrol and most of the resveratrol derivatives were applied. Lower adhesion inhibition was observed for the bacteria with higher adherence potential, L. monocytogenes (≥20%). Resveratrol-3-O-(6'-O-butanoyl)-β-D-glucopyranoside (BUT) (50 and 100 μM) and resveratrol-3-O-(6'-O-octanoyl)-β-D-glucopyranoside (OCT) (50 μM) reduced IL-8 secretion by 100%. These results suggest that one mechanism for the beneficial attributes of resveratrol and especially the derivatives BUT and OCT could be the ability to reduce the adhesion and consequent pro-inflammatory cytokine production in intestinal epithelial cells in response to pathogen adhesion. The potential use of these compounds in the prevention of foodborne infections, intestinal homeostasis loss, and inflammatory bowel diseases could be another step in finding coadjuvants or alternatives to antibiotic treatments.
authors: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC , 30100 Campus de Espinardo, Murcia, Spain.
authors: María V
authors: MV
authors: Selma
authors: Mar
authors: M
authors: Larrosa
authors: David
authors: D
authors: Beltrán
authors: Ricardo
authors: R
authors: Lucas
authors: Juan C
authors: JC
authors: Morales
authors: Francisco
authors: F
authors: Tomás-Barberán
authors: Juan C
authors: JC
authors: Espín
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D018938:Caco-2 Cells; D015169:Colony Count, Microbial; D019453:Escherichia coli O157 / Q000187:drug effects*; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D019073:HT29 Cells; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008089:Listeria monocytogenes / Q000187:drug effects*; D000077185:Resveratrol; D012486:Salmonella typhimurium / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D016209:Interleukin-8; D013267:Stilbenes; D000077185:Resveratrol