title: Poly (D,L-lactic-co-glycolide) nanoparticles for the improved therapeutic efficacy of all-trans-retinoic acid: a study of acute myeloid leukemia (AML) cell differentiation in vitro.
abstract: All-trans-retinoic acid reverses malignant cell growth and induces cell differentiation and apoptosis. Poor aqueous solubility and uncertain bioavailability are the limiting factors for using all-trans-retinoic acid for tumor therapy. The objective of present study was to encapsulate the hydrophobic drug all-trans-retinoic acid in the polymer poly (lactide-coglycolide). The encapsulation was expected to improve the bioavailability and solubility of the drug. Oil in water single emulsion solvent evaporation technique used for the preparation efficiently encapsulated about 60% of the drug. The drug release profile showed a biphasic pattern with 70% of the drug being released in first 48 hrs and the residual drug showing a slow controlled release reaching up to 8 days. The particle size of 150-200 nm as determined with TEM was ideal for tumor targeting. All-trans-retinoic acid loaded nanoparticles were efficient to induce differentiation and blocked the proliferation of HL-60 cells invitro. These studies also revealed that the dosage of drug required for the therapeutic effects have been reduced efficiently. Our studies thereby demonstrate that Poly (lactide-co-glycolide) based nanoparticles may be efficient for parenteral administration of the drug.
authors: Chemical Biology, Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram-695 014, Kerala, India.
authors: Aswathy Mary
authors: AM
authors: Simon
authors: Sankar
authors: S
authors: Jagadeeshan
authors: Emimol
authors: E
authors: Abraham
authors: Ashalatha
authors: A
authors: Akhilandeshwaran
authors: Jisha J
authors: JJ
authors: Pillai
authors: Nisha Asok
authors: NA
authors: Kumar
authors: Asha Nair
authors: AN
authors: Sivakumari
authors: Gopalakrishnapillai Sankaramangalam Vinod
authors: GS
authors: Kumar
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002454:Cell Differentiation; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D004339:Drug Compounding; D004655:Emulsions; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000473:pathology*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011098:Polyglactin 910 / Q000737:chemistry*; D012995:Solubility; D014212:Tretinoin / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D004655:Emulsions; D011098:Polyglactin 910; D014212:Tretinoin