title: The choline oxidase gene codA confers salt tolerance to transgenic Eucalyptus globulus in a semi-confined condition.
abstract: The performance of tree species is influenced by environmental factors and growth stages. To evaluate the practical performance of transgenic tree species, it is insufficient to grow small, young trees under controlled conditions, such as in a growth chamber. Three transgenic Eucalyptus globulus lines, carrying the choline oxidase gene, were investigated for their salt tolerance and expression of the transgene at the young plantlet stage in a special netted-house. To clarify the characteristics at the young as well during the later stages, salt tolerance and the properties of the transgenic lines at large juvenile and adult stages were evaluated in the special netted-house. All transgenic lines showed high glycinebetaine content, particularly in young leaves. Trees of the transgenic line 107-1 showed low damage because of salinity stress based on the results from the chlorophyll analysis and malondialdehyde content, and they survived the high-salt-shock treatment at the large juvenile and adult stages. Only this line showed salt tolerance at all stages in the special netted-house. In this evaluation in the special netted-house, the tolerant line among young plantlets might perform better at all stages. Since evaluation in these special netted-house mimics field evaluation, line 107-1 is a potential tolerant line.
authors: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
authors: Xiang
authors: X
authors: Yu
authors: Akira
authors: A
authors: Kikuchi
authors: Etsuko
authors: E
authors: Matsunaga
authors: Yoshihiko
authors: Y
authors: Morishita
authors: Kazuya
authors: K
authors: Nanto
authors: Nozomu
authors: N
authors: Sakurai
authors: Hideyuki
authors: H
authors: Suzuki
authors: Daisuke
authors: D
authors: Shibata
authors: Teruhisa
authors: T
authors: Shimada
authors: Kazuo N
authors: KN
authors: Watanabe
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D002734:Chlorophyll / Q000235:genetics / Q000378:metabolism; D005052:Eucalyptus / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D008315:Malondialdehyde / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*; D019076:Transgenes
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll; D012965:Sodium Chloride; D008315:Malondialdehyde; D000429:Alcohol Oxidoreductases; C019888:choline oxidase