title: Rapid and high-throughput detection of highly pathogenic bacteria by Ibis PLEX-ID technology.
abstract: In this manuscript, we describe the identification of highly pathogenic bacteria using an assay coupling biothreat group-specific PCR with electrospray ionization mass spectrometry (PCR/ESI-MS) run on an Ibis PLEX-ID high-throughput platform. The biothreat cluster assay identifies most of the potential bioterrorism-relevant microorganisms including Bacillus anthracis, Francisella tularensis, Yersinia pestis, Burkholderia mallei and pseudomallei, Brucella species, and Coxiella burnetii. DNA from 45 different reference materials with different formulations and different concentrations were chosen and sent to a service screening laboratory that uses the PCR/ESI-MS platform to provide a microbial identification service. The standard reference materials were produced out of a repository built up in the framework of the EU funded project "Establishment of Quality Assurances for Detection of Highly Pathogenic Bacteria of Potential Bioterrorism Risk" (EQADeBa). All samples were correctly identified at least to the genus level.
authors: Robert Koch-Institut, Berlin, Germany.
authors: Daniela
authors: D
authors: Jacob
authors: Uschi
authors: U
authors: Sauer
authors: Roberta
authors: R
authors: Housley
authors: Cicely
authors: C
authors: Washington
authors: Kristin
authors: K
authors: Sannes-Lowery
authors: David J
authors: DJ
authors: Ecker
authors: Rangarajan
authors: R
authors: Sampath
authors: Roland
authors: R
authors: Grunow
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D050296:Microbial Viability / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004269:DNA, Bacterial