title: The IGR-CaP1 xenograft model recapitulates mixed osteolytic/blastic bone lesions observed in metastatic prostate cancer.
abstract: Bone metastases have a devastating impact on quality of life and bone pain in patients with prostate cancer and decrease survival. Animal models are important tools in investigating the pathogenesis of the disease and in developing treatment strategies for bone metastases, but few animal models recapitulate spontaneous clinical bone metastatic spread. In the present study, IGR-CaP1, a new cell line derived from primary prostate cancer, was stably transduced with a luciferase-expressing viral vector to monitor tumor growth in mice using bioluminescence imaging. The IGR-CaP1 tumors grew when subcutaneously injected or when orthotopically implanted, reconstituted the prostate adenocarcinoma with glandular acini-like structures, and could disseminate to the liver and lung. Bone lesions were detected using bioluminescence imaging after direct intratibial or intracardiac injections. Anatomic bone structure assessed using high-resolution computed tomographic scans showed both lytic and osteoblastic lesions. Technetium Tc 99m methylene diphosphonate micro single-photon emission computed tomography confirmed the mixed nature of the lesions and the intensive bone remodeling. We also identified an expression signature for responsiveness of IGR-CaP1 cells to the bone microenvironment, namely expression of CXCR4, MMP-9, Runx2, osteopontin, osteoprotegerin, ADAMTS14, FGFBP2, and HBB. The IGR-CaP1 cell line is a unique model derived from a primary tumor, which can reconstitute human prostate adenocarcinoma in animals and generate experimental bone metastases, providing a novel means for understanding the mechanisms of bone metastasis progression and allowing preclinical testing of new therapies.
authors: Prostate Cancer Group, INSERM U981, Institut Gustave Roussy, Villejuif, France.
authors: Nader
authors: N
authors: Al Nakouzi
authors: Olivia
authors: O
authors: Bawa
authors: Alain
authors: A
authors: Le Pape
authors: Stéphanie
authors: S
authors: Lerondel
authors: Catherine
authors: C
authors: Gaudin
authors: Paule
authors: P
authors: Opolon
authors: Patrick
authors: P
authors: Gonin
authors: Karim
authors: K
authors: Fizazi
authors: Anne
authors: A
authors: Chauchereau
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D000818:Animals; D001859:Bone Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000556:secondary*; D016723:Bone Remodeling; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D010014:Osteolysis; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D014183:Transplantation, Heterologous; D047368:Tumor Burden