title: BOLA1 is an aerobic protein that prevents mitochondrial morphology changes induced by glutathione depletion.
abstract: OBJECTIVE
  The BolA protein family is widespread among eukaryotes and bacteria. In Escherichia coli, BolA causes a spherical cell shape and is overexpressed during oxidative stress. Here we aim to elucidate the possible role of its human homolog BOLA1 in mitochondrial morphology and thiol redox potential regulation.
  
  
  RESULTS
  We show that BOLA1 is a mitochondrial protein that counterbalances the effect of L-buthionine-(S,R)-sulfoximine (BSO)-induced glutathione (GSH) depletion on the mitochondrial thiol redox potential. Furthermore, overexpression of BOLA1 nullifies the effect of BSO and S-nitrosocysteine on mitochondrial morphology. Conversely, knockdown of the BOLA1 gene increases the oxidation of mitochondrial thiol groups. Supporting a role of BOLA1 in controlling the mitochondrial thiol redox potential is that BOLA1 orthologs only occur in aerobic eukaryotes. A measured interaction of BOLA1 with the mitochondrial monothiol glutaredoxin GLRX5 provides hints for potential mechanisms behind BOLA1's effect on mitochondrial redox potential. Nevertheless, we have no direct evidence for a role of GLRX5 in BOLA1's function.
  
  
  METHODS
  We implicate a new protein, BOLA1, in the regulation of the mitochondrial thiol redox potential.
  
  
  CONCLUSIONS
  BOLA1 is an aerobic, mitochondrial protein that prevents mitochondrial morphology aberrations induced by GSH depletion and reduces the associated oxidative shift of the mitochondrial thiol redox potential.
authors: Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
authors: Peter
authors: P
authors: Willems
authors: Bas F J
authors: BF
authors: Wanschers
authors: John
authors: J
authors: Esseling
authors: Radek
authors: R
authors: Szklarczyk
authors: Urszula
authors: U
authors: Kudla
authors: Isabel
authors: I
authors: Duarte
authors: Marleen
authors: M
authors: Forkink
authors: Marco
authors: M
authors: Nooteboom
authors: Herman
authors: H
authors: Swarts
authors: Jolein
authors: J
authors: Gloerich
authors: Leo
authors: L
authors: Nijtmans
authors: Werner
authors: W
authors: Koopman
authors: Martijn A
authors: MA
authors: Huynen
mesh_terms: D019328:Buthionine Sulfoximine / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000502:physiology*; D010084:Oxidation-Reduction
chemical_list: C533595:BOLA1 protein, human; D024101:Mitochondrial Proteins; D019328:Buthionine Sulfoximine; D005978:Glutathione