title: Direct electrochemistry of hemoglobin immobilized in chitosan-room temperature ionic liquid film and application in its interaction with 3,4'-bis-(4-hydro-3-xycoumarin)-2,5-hexanediol.
abstract: The direct electrochemistry of hemoglobin (Hb) and application in its interaction with 3,4'-bis-(4-hydro-3-xycoumarin)-2,5-hexanediol (HCH) based on the Hb immobilized in chitosan-room temperature ionic liquid film were investigated by means of cyclic voltammetry, differential pulse voltammetry and amperometry. The binding ratio m and binding constant between HCH and Hb were estimated as 2 and 3.46 M(-1), respectively. The amperometric response showed a linear dependence on the concentration of HCH with the detection limit of 0.06 μM. In addition, the amperometry was a novel method in the field of binding studies and might be taken into account for future binding studies. At last a sensitive and convenient electrochemical method was proposed for the determination of HCH.
authors: College of Sciences, Xi'an University of Architecture and Technology, Xi'an, PR China. dshiney2004@yahoo.com.cn
authors: Sheying
authors: S
authors: Dong
authors: Zhiqin
authors: Z
authors: Li
authors: Zhuqing
authors: Z
authors: Yu
authors: Yuanzhen
authors: Y
authors: Zhou
authors: Hongsheng
authors: H
authors: Tang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D003374:Coumarins / Q000032:analysis*; D055664:Electrochemical Techniques; D004566:Electrodes; D006018:Glycols / Q000032:analysis*; D017320:HIV Protease Inhibitors / Q000032:analysis*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins; D052578:Ionic Liquids / Q000737:chemistry*; D057230:Limit of Detection; D013696:Temperature
chemical_list: C576529:3,4'-bis-(4-hydroxycoumarin-3-yl)-2,5-hexanediol; D003374:Coumarins; D006018:Glycols; D017320:HIV Protease Inhibitors; D006454:Hemoglobins; D055767:Immobilized Proteins; D052578:Ionic Liquids; D048271:Chitosan; D006861:Hydrogen Peroxide