title: Nanoassisted laser desorption-ionization-MS imaging of tumors.
abstract: The ability of nanoassisted laser desorption-ionization mass spectrometry (NALDI-MS) imaging to provide selective chemical monitoring with proper spatial distribution of lipid profiles from tumor tissues after plate imprinting has been tested. NALDI-MS imaging identified and mapped several potential lipid biomarkers in a murine model of melanoma tumor (inoculation of B16/F10 cells). It also confirmed that the in vivo treatment of tumor bearing mice with synthetic supplement containing phosphoethanolamine (PHO-S) promoted an accentuated decrease in relative abundance of the tumor biomarkers. NALDI-MS imaging is a matrix-free LDI protocol based on the selective imprinting of lipids in the NALDI plate followed by the removal of the tissue. It therefore provides good quality and selective chemical images with preservation of spatial distribution and less interference from tissue material. The test case described herein illustrates the potential of chemically selective NALDI-MS imaging for biomarker discovery.
authors: ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas-UNICAMP, Campinas, SP, Brazil.
authors: Alessandra
authors: A
authors: Tata
authors: Anna Maria A P
authors: AM
authors: Fernandes
authors: Vanessa G
authors: VG
authors: Santos
authors: Rosana M
authors: RM
authors: Alberici
authors: Dioneia
authors: D
authors: Araldi
authors: Carlos A
authors: CA
authors: Parada
authors: Wellington
authors: W
authors: Braguini
authors: Luciana
authors: L
authors: Veronez
authors: Gabriela
authors: G
authors: Silva Bisson
authors: Felippe H Z
authors: FH
authors: Reis
authors: Luciane C
authors: LC
authors: Alberici
authors: Marcos N
authors: MN
authors: Eberlin
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D004983:Ethanolamines / Q000627:therapeutic use; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D036103:Nanotechnology; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014184:Transplantation, Homologous
chemical_list: D014408:Biomarkers, Tumor; D004983:Ethanolamines; C005448:phosphorylethanolamine