title: Plasmid-mediated quinolone resistance mechanisms in ESBL positive Escherichia coli and Klebsiella pneumoniae strains at a Tertiary-Care Hospital in Turkey.
abstract: Plasmid-mediated quinolone resistance mechanisms in extended spectrum beta-lactamase positive and quinolone resistant Escherichia coli and Klebsiella pneumoniae strains isolated from Ege University Hospital were investigated. The presence of qnrA, qnrB, qnrS, aac(6')-Ib  and qepA genes were detected by PCR and the products were sequenced. Clonal relationship of isolates was determined by REP-PCR and mutations in gyrA and parC genes were investigated in representative strains. aac(6')-Ib-cr, qnrB and qnrS genes were  detected in both E. coli and K. pneumoniae strains, but qnrA detected only in K. pneumoniae strains. qepA determinant is detected in an E. coli strain first time in Turkey. Mutations were observed in both gyrA and parC genes of all representative  nalidixic acid and ciprofloxacin resistant E. coli isolates but no mutation was found in parC genes of E. coli and K. pneumoniae strains that were resistant to only nalidixic acid.
authors: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, Izmir, Turkey.
authors: Mine
authors: M
authors: Hoşgör-Limoncu
authors: Bayrı
authors: B
authors: Eraç
authors: Ayşe Nur
authors: AN
authors: Yurtman
authors: Söhret
authors: S
authors: Aydemir
mesh_terms: D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D006761:Hospitals; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015363:Quinolones / Q000494:pharmacology*; D014421:Turkey; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D015363:Quinolones; D001618:beta-Lactamases