title: High-throughput single-microparticle imaging flow analyzer.
abstract: Optical microscopy is one of the most widely used diagnostic methods in scientific, industrial, and biomedical applications. However, while useful for detailed examination of a small number (< 10,000) of microscopic entities, conventional optical microscopy is incapable of statistically relevant screening of large populations (> 100,000,000) with high precision due to its low throughput and limited digital memory size. We present an automated flow-through single-particle optical microscope that overcomes this limitation by performing sensitive blur-free image acquisition and nonstop real-time image-recording and classification of microparticles during high-speed flow. This is made possible by integrating ultrafast optical imaging technology, self-focusing microfluidic technology, optoelectronic communication technology, and information technology. To show the system's utility, we demonstrate high-throughput image-based screening of budding yeast and rare breast cancer cells in blood with an unprecedented throughput of 100,000 particles/s and a record false positive rate of one in a million.
authors: Department of Electrical Engineering, University of California, Los Angeles, CA 90095, USA. goda@ee.ucla.edu
authors: Keisuke
authors: K
authors: Goda
authors: Ali
authors: A
authors: Ayazi
authors: Daniel R
authors: DR
authors: Gossett
authors: Jagannath
authors: J
authors: Sadasivam
authors: Cejo K
authors: CK
authors: Lonappan
authors: Elodie
authors: E
authors: Sollier
authors: Ali M
authors: AM
authors: Fard
authors: Soojung Claire
authors: SC
authors: Hur
authors: Jost
authors: J
authors: Adam
authors: Coleman
authors: C
authors: Murray
authors: Chao
authors: C
authors: Wang
authors: Nora
authors: N
authors: Brackbill
authors: Dino
authors: D
authors: Di Carlo
authors: Bahram
authors: B
authors: Jalali
mesh_terms: D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods*; D004718:Saccharomycetales