title: Automated radiosynthesis of [(18)F]ML-10, a PET radiotracer dedicated to apoptosis imaging, on a TRACERLab FX-FN module.
abstract: OBJECTIVE
  [(18)F]ML-10 is the most advanced radiopharmaceutical for the clinical imaging of the apoptosis phenomenon by PET. The preparation of this radiopharmaceutical on a commercial radiosynthesis module and the requested quality controls for its release are presented herein.
  
  
  METHODS
  ML-10 as reference and its mesyloxy derivative as precursor for labelling with fluorine-18 were prepared. [(18)F]ML-10 was synthesized via a [(18)F]fluorine-de-mesyloxy aliphatic nucleophilic substitution via a GE TRACERLab® FX-FN module. Quality controls were performed.
  
  
  RESULTS
  The labelling precursor was obtained in a four step synthesis in 28 % overall yield affording ML-10 in two steps (88 % yield). Pure [(18)F]ML-10 was obtained with a decay corrected yield of 39.8 % ± 8.4 % (n = 7) in 70 min and a specific activity of 235 ± 85 GBq/μmol at the end of synthesis.
  
  
  CONCLUSIONS
  [(18)F]ML-10 was prepared on a widely available automated module and passed the quality control. A LC/MS method was developed to measure specific radioactivity.
authors: CEA, I2BM, LDM-TEP, UMR 6301 ISTCT, GIP Cyceron, 14074 Caen, France. sobrio@cyceron.fr
authors: Franck
authors: F
authors: Sobrio
authors: Marie
authors: M
authors: Médoc
authors: Ludovic
authors: L
authors: Martial
authors: Jérôme
authors: J
authors: Delamare
authors: Louisa
authors: L
authors: Barré
mesh_terms: D017209:Apoptosis / Q000502:physiology; D007700:Kinetics; D008764:Methylmalonic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D057054:Molecular Imaging / Q000379:methods; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: C532430:5-fluoropentyl-2-methylmalonic acid; D019275:Radiopharmaceuticals; D008764:Methylmalonic Acid