title: Analysis of the mildiomycin biosynthesis gene cluster in Streptoverticillum remofaciens ZJU5119 and characterization of MilC, a hydroxymethyl cytosyl-glucuronic acid synthase.
abstract: Mildiomycin (MIL) is a peptidyl-nucleoside antibiotic produced by Streptoverticillum remofaciens ZJU5119 that exhibits strong inhibitory activity against powdery mildew. The entire MIL biosynthesis gene cluster was cloned and expressed in Streptomyces lividans 1326. Systematic gene disruptions narrowed down the cluster to 16 functional ORFs and identified the boundaries of the gene cluster. A putative cytosylglucuronic acid (CGA) synthase gene, milC, was disrupted in Sv. remofaciens and heterologously expressed in E. coli. An in vitro assay revealed that purified MilC could utilize either cytosine or hydroxymethylcytosine as substrate to yield CGA or hydroxymethyl-CGA (HM-CGA), respectively. MilG is believed to be a key enzyme in the MIL biosynthesis pathway and contains the C(XXX)C(XX)C motif characteristic of members of the radical S-adenosyl methionine (SAM) superfamily. Disruption of milG leads to accumulation of HM-CGA. Labeling experiments with (13)C(6)-L-arginine indicated that decarboxylation at C5 of the pyranoside ring was coupled with the attachment of 5-guanidino-2,4-dihydroxyvalerate side chain through C-C bond formation. In contrast, exogenous (13)C(6)-labeled 4-hydroxy-L-arginine was not incorporated into the MIL structure. Comparative analysis of the 16 MIL ORFs with counterparts involved in the biosynthesis of the structurally similar compound blasticidin S, along with the results above, provide insight into the complete MIL biosynthetic pathway.
authors: State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China.
authors: Jun
authors: J
authors: Wu
authors: Li
authors: L
authors: Li
authors: Zixin
authors: Z
authors: Deng
authors: T Mark
authors: TM
authors: Zabriskie
authors: Xinyi
authors: X
authors: He
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: D003596:Cytosine; C086034:cytosine-UDP-glucuronosyltransferase; D014453:Glucuronosyltransferase; C017539:mildiomycin