title: Crystallization and preliminary X-ray analysis of a novel halotolerant feruloyl esterase identified from a soil metagenomic library.
abstract: Feruloyl esterase cleaves the ester linkage formed between ferulic acid and polysaccharides in plant cell walls and thus has wide potential industrial applications. A novel feruloyl esterase (EstF27) identified from a soil metagenomic library was crystallized and a complete data set was collected from a single cooled crystal using an in-house X-ray source. The crystal diffracted to 2.9 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 94.35, b = 106.19, c = 188.51 Å, α = β = γ = 90.00°. A Matthews coefficient of 2.55 Å(3) Da(-1), with a corresponding solvent content of 51.84%, suggested the presence of ten protein subunits in the asymmetric unit.
authors: School of Pharmaceutical Sciences, Sun Yat-sen University, Higher Education Mega Center, Guangzhou, Guangdong 510006, People's Republic of China.
authors: Shang-ke
authors: SK
authors: Chen
authors: Kui
authors: K
authors: Wang
authors: Yuhuan
authors: Y
authors: Liu
authors: Xiaopeng
authors: X
authors: Hu
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D056186:Metagenomics; D012987:Soil
chemical_list: D012987:Soil; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase