title: Transgene mobilization and regulatory uncertainty for non-GE fruit products of transgenic rootstocks.
abstract: Genetically engineered (GE) rootstocks may offer some advantages for biotechnology applications especially in woody perennial crops such as grape or walnut. Transgrafting combines horticultural grafting practices with modern GE methods for crop improvement. Here, a non-GE conventional scion (upper stem portion) is grafted onto a transgenic GE rootstock. Thus, the scion does not contain the genetic modification present in the rootstock genome. We examined transgene presence in walnut and tomato GE rootstocks and non-GE fruit-bearing scions. Mobilization of transgene DNA, protein, and mRNA across the graft was not detected. Though transgenic siRNA mobilization was not observed in grafted tomatoes or walnut scions, transgenic siRNA signal was detected in walnut kernels. Prospective benefits from transgrafted plants include minimized risk of GE pollen flow (Lev-Yadun and Sederoff, 2001), possible use of more than one scion per approved GE rootstock which could help curb the estimated US$136 million (CropLife International, 2011) cost to bring a GE crop to international markets, as well as potential for improved consumer and market acceptance since the consumable product is not itself GE. Thus, transgrafting provides an alternative option for agricultural industries wishing to expand their biotechnology portfolio.
authors: Morrison and Foerster LLC, 425 Market Street, San Francisco, CA 94111, USA.
authors: Victor M
authors: VM
authors: Haroldsen
authors: Cecilia L
authors: CL
authors: Chi-Ham
authors: Alan B
authors: AB
authors: Bennett
mesh_terms: D005638:Fruit / Q000235:genetics*; D005818:Genetic Engineering / Q000331:legislation & jurisprudence* / Q000379:methods*; D031324:Juglans / Q000235:genetics / Q000254:growth & development; D018551:Solanum lycopersicum / Q000235:genetics / Q000254:growth & development; D018517:Plant Roots / Q000235:genetics*; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D012926:Social Control, Formal; D019076:Transgenes / Q000235:genetics*; D035501:Uncertainty
chemical_list: D018749:RNA, Plant; D034741:RNA, Small Interfering