title: Biotransformation of hydroxycoumarin derivatives by cultured suspension cells of Catharanthus roseus.
abstract: Using 7-hydroxy-4-methylcoumarin (1), 7-hydroxy-4-phenylcoumarin (2), 5,7-dihydroxy-4-methylcoumarin (3), 7,8-dihydroxycoumarin (4) as substrates, three new compounds, 4-methylcoumarin-7-O-beta-D-xylopyranosyl (1 --> 6) beta-D-glucopyranoside (1b), 4-phenylcoumarin-7-O-beta-D-glucopyranosyl (1 --> 6) beta-D-glucopyranoside (2b), 4-methylcoumarin-5,7-O-beta-D-diglucoside (3b), along with four known compounds (1a, 2a, 3a and 4a) were biosynthesized by the cell suspension cultures of Catharanthus roseus as biocatalyst. Their structures were elucidated by analysis of the physicochemical properties and 1H NMR, 13C NMR, HMBC and ESI-MS spectra. Besides, the results also showed that this novel biocatalyst system of C. roseus cell cultures has a great capacity of regio-selective glycosylation to the hydroxyl group of the exogenous substrates. Co-culture time curves on conversion were also established.
authors: College of Pharmacy, Jinan University, Guangzhou, China.
authors: Bailin
authors: B
authors: Xue
authors: Liangbin
authors: L
authors: Zhou
authors: Jinwei
authors: J
authors: Liu
authors: Rongmin
authors: R
authors: Yu
mesh_terms: D001711:Biotransformation; D030008:Catharanthus / Q000378:metabolism*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D018920:Coculture Techniques; D003374:Coumarins / Q000378:metabolism*; D004121:Dimethyl Sulfoxide; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D003374:Coumarins; D012997:Solvents; D004121:Dimethyl Sulfoxide