title: Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions.
abstract: Bacteroides thetaiotaomicron VPI-5482 harbors a gene encoding a putative cycloisomaltooligosaccharide glucanotransferase (BT3087) belonging to glycoside hydrolase family 66. The goal of the present study was to characterize the catalytic properties of this enzyme. Therefore, we expressed BT3087 (recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482) in Escherichia coli and determined that recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482 preferentially synthesized isomaltotetraose and isomaltooligosaccharides (degree of polymerization > 4) from dextran. The enzyme also generated large cyclic isomaltooligosaccharides early in the reaction. We conclude that members of the glycoside hydrolase 66 family may be classified into three types: (a) endo-dextranases, (b) dextranases possessing weak cycloisomaltooligosaccharide glucanotransferase activity, and (c) cycloisomaltooligosaccharide glucanotransferases.
authors: Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
authors: Young-Min
authors: YM
authors: Kim
authors: Eiji
authors: E
authors: Yamamoto
authors: Min-Sun
authors: MS
authors: Kang
authors: Hiroyuki
authors: H
authors: Nakai
authors: Wataru
authors: W
authors: Saburi
authors: Masayuki
authors: M
authors: Okuyama
authors: Haruhide
authors: H
authors: Mori
authors: Kazumi
authors: K
authors: Funane
authors: Mitsuru
authors: M
authors: Momma
authors: Zui
authors: Z
authors: Fujimoto
authors: Mikihiko
authors: M
authors: Kobayashi
authors: Doman
authors: D
authors: Kim
authors: Atsuo
authors: A
authors: Kimura
mesh_terms: D000595:Amino Acid Sequence; D001439:Bacteroides / Q000201:enzymology*; D055162:Biocatalysis; D003001:Cloning, Molecular; D003500:Cyclization; D003911:Dextrans / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D003911:Dextrans; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases