title: [Breakpoints located by sequence tagged sites of AZFc microdeletion in Chinese Han population].
abstract: OBJECTIVE
  To investigate the breakpoints of the azoospermia factor c (AZFc) microdeletion in Chinese Han population.
  
  
  METHODS
  We detected 9 sequence tagged sites (sY84, sY86, sY127, sY134, sY152, sY145, sY255, sY254 and sY157) to confirm AZFc microdeletions in the Y chromosome for patients with severe oligozoospermia or non-obstructive azoospermia by multiplex polymerase reaction. To locate the breakpoints of AZFc microdeletions, we analyzed 192 patients with sY255, sY254 and sY157 dele- ted by detecting sY1191, sY1197, sY1054, sY1125 and sY1206, respectively.
  
  
  RESULTS
  Five breaking patterns were found in the 192 patients with sY255, sY254 and sY157 deleted, among which the common ones were sY1197(+), sY1191(-), sY1054(-), sY1206(-) and sY1125(+), which accounted fro 54.17% (104/192), sY1197(+), sY1191(+), sY1206(-), sY1054(-) and sY1125(+), which constituted 28.12% (54/192), sY1197(+), sY1191(-), sY1206(-), sY1054(+) and sY1125 (+), which made up 14.58% (28/192). The proximal breakpoint located between sY1197 and sY1191 was 70.83% of AZFc microdeletions, and the distant breakpoint located between sY1054 and sY1125 was 82.29%.
  
  
  CONCLUSIONS
  There are 5 breaking patterns of AZFc microdeletions in Chinese Han population, the proximal and distant breakpoints mostly located at the replicons b2 and b4, respectively.
authors: Shandong Provincial Key Laboratory for Healthy Birth Techniques, Shandong Institute of Planned Parenthood Research, Jinan, Shandong 250002, China.
authors: Qing
authors: Q
authors: Wu
authors: Lei-Guang
authors: LG
authors: Wang
authors: Bin
authors: B
authors: Wu
authors: Yi
authors: Y
authors: Qiu
authors: Yan
authors: Y
authors: Xu
authors: Miao
authors: M
authors: Liu
authors: Peng
authors: P
authors: Wang
authors: Yao
authors: Y
authors: Yuan
authors: Hui-Juan
authors: HJ
authors: Shi
mesh_terms: D044466:Asian People / Q000235:genetics; D053713:Azoospermia / Q000235:genetics*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D016324:Sequence Tagged Sites