title: A mechanistic insight into the amyloidogenic structure of hIAPP peptide revealed from sequence analysis and molecular dynamics simulation.
abstract: A collective approach of sequence analysis, phylogenetic tree and in silico prediction of amyloidogenecity using bioinformatics tools have been used to correlate the observed species-specific variations in IAPP sequences with the amyloid forming propensity. Observed substitution patterns indicate that probable changes in local hydrophobicity are instrumental in altering the aggregation propensity of the peptide. In particular, residues at 17th, 22nd and 23rd positions of the IAPP peptide are found to be crucial for amyloid formation. Proline25 primarily dictates the observed non-amyloidogenecity in rodents. Furthermore, extensive molecular dynamics simulation of 0.24 μs have been carried out with human IAPP (hIAPP) fragment 19-27, the portion showing maximum sequence variation across different species, to understand the native folding characteristic of this region. Principal component analysis in combination with free energy landscape analysis illustrates a four residue turn spanning from residue 22 to 25. The results provide a structural insight into the intramolecular β-sheet structure of amylin which probably is the template for nucleation of fibril formation and growth, a pathogenic feature of type II diabetes.
authors: Saroj Mohan Institute of Technology, Guptipara, Hooghly, Pin 712512, India. sandipanchakraborty.13@gmail.com
authors: Sandipan
authors: S
authors: Chakraborty
authors: Barnali
authors: B
authors: Chatterjee
authors: Soumalee
authors: S
authors: Basu
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D058228:Islet Amyloid Polypeptide / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D010802:Phylogeny; D025341:Principal Component Analysis; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013816:Thermodynamics
chemical_list: D058228:Islet Amyloid Polypeptide