title: Crystallization and preliminary crystallographic studies of CCM3 in complex with the C-terminal domain of MST4.
abstract: MST4 is a member of the GCKIII kinases. The interaction between cerebral cavernous malformation 3 (CCM3) and GCKIII kinases plays a critical role in cardiovascular development and in cerebral cavernous malformations. The complex of CCM3 and the C-terminal domain of MST4 has been constructed, purified and crystallized, and a diffraction data set has been collected to 2.4 Å resolution. The crystal of the CCM3-MST4 C-terminal domain complex belonged to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = 69.10, b = 69.10, c = 117.57 Å.
authors: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors: Xueyong
authors: X
authors: Xu
authors: Xiaoyan
authors: X
authors: Wang
authors: Jingjin
authors: J
authors: Ding
authors: Da-Cheng
authors: DC
authors: Wang
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000737:chemistry* / Q000378:metabolism; D000099317:Hippo Kinases
chemical_list: D051017:Apoptosis Regulatory Proteins; D008565:Membrane Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; C495390:PDCD10 protein, human; C430908:STK26 protein, human; D000099317:Hippo Kinases