title: Evaluation of envelope glycoprotein E(rns) of an atypical bovine pestivirus as antigen in a microsphere immunoassay for the detection of antibodies against bovine viral diarrhea virus 1 and atypical bovine pestivirus.
abstract: Atypical bovine pestiviruses are related antigenically and phylogenetically to bovine viral diarrhea viruses (BVDV-1 and BVDV-2), and may cause the same clinical manifestations in animals. Glycoprotein E(rns) of an atypical bovine pestivirus Th/04_KhonKaen was produced in a baculovirus expression system and was purified by affinity chromatography. The recombinant E(rns) protein was used as an antigen in a microsphere immunoassay for the detection of antibodies against BVDV-1 and atypical bovine pestivirus. The diagnostic performance of the new method was evaluated by testing a total of 596 serum samples, and the assay was compared with enzyme-linked immunosorbent assay (ELISA). Based on the negative/positive cut-off median fluorescence intensity (MFI) value of 2800, the microsphere immunoassay had a sensitivity of 100% and specificity of 100% compared to ELISA. The immunoassay was able to detect antibodies against both BVDV-1 and the atypical pestivirus. This novel microsphere immunoassay has the potential to be multiplexed for simultaneous detection of antibodies against different bovine pathogens in a high-throughput and economical way.
authors: Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences (SLU), SE-751 89 Uppsala, Sweden.
authors: Balaje
authors: B
authors: Vijayaraghavan
authors: Hongyan
authors: H
authors: Xia
authors: Rajiv
authors: R
authors: Harimoorthy
authors: Lihong
authors: L
authors: Liu
authors: Sándor
authors: S
authors: Belák
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D016367:Baculoviridae; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000097:blood / Q000276:immunology; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000276:immunology / Q000302:isolation & purification*; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology* / Q000302:isolation & purification; D005822:Genetic Vectors; D007118:Immunoassay / Q000379:methods* / Q000662:veterinary; D008863:Microspheres; D010802:Phylogeny; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D061987:Sf9 Cells; D014162:Transfection; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins