title: Target genes of microsatellite sequences in head and neck squamous cell carcinoma: mononucleotide repeats are not detected.
abstract: Microsatellite instability (MSI) is detected in a wide variety of tumors. It is thought that mismatch repair gene mutation or inactivation is the major cause of MSI. Microsatellite sequences are predominantly distributed in intergenic or intronic DNA. However, MSI is found in the exonic sequences of some genes, causing their inactivation. In this report, we searched GenBank for candidate genes containing potential MSI sequences in exonic regions. Twenty seven target genes were selected for MSI analysis. Instability was found in 70% of these genes (14/20) with head and neck squamous cell carcinoma (HNSCC). Interestingly, no instability was detected in mononucleotide repeats in genes or in intergenic sequences. We conclude that instability of mononucleotide repeats is a rare event in HNSCC. High MSI phenotype in young HNSCC patients is limited to noncoding regions only. MSI percentage in HNSCC tumor is closely related to the repeat type, repeat location and patient's age.
authors: Department of Pathology, Jilin University, The key laboratory of Pathobiology, Ministry of Education, 126 Xinmin Street, Changchun, Jilin 130021, PR China.
authors: Yimin
authors: Y
authors: Wang
authors: Xuejuan
authors: X
authors: Liu
authors: Yulin
authors: Y
authors: Li
mesh_terms: D000328:Adult; D000367:Age Factors; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D030561:Databases, Nucleic Acid; D005260:Female; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D053842:Microsatellite Instability; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D017931:DNA Primers; D004273:DNA, Neoplasm