title: Photocontrolled nanoparticles for on-demand release of proteins.
abstract: We describe here light-regulated swelling and degradation features of polymeric nanoparticles that are produced using an inverse microemulsion polymerization method. We demonstrate the phototriggered release characteristics of the nanoparticles by sequestering protein molecules and releasing them using light as a trigger. Furthermore, the intracellular translocation of the nanoparticles, along with its fluorescent protein payload, was achieved using a cell-penetrating peptide-based surface modification. We expect that the noncovalent encapsulation of proteins using nanoparticles and their photo triggered release using an external light would provide opportunities for achieving intracellular release of molecular therapeutics for on-demand requirements.
authors: Department of Chemical and Biological Engineering and the Howard Hughes Medical Institute, University of Colorado at Boulder, Boulder, Colorado 80303, United States.
authors: Malar A
authors: MA
authors: Azagarsamy
authors: Daniel L
authors: DL
authors: Alge
authors: Srinidhi J
authors: SJ
authors: Radhakrishnan
authors: Mark W
authors: MW
authors: Tibbitt
authors: Kristi S
authors: KS
authors: Anseth
mesh_terms: D016475:3T3 Cells; D000179:Acrylates / Q000737:chemistry; D000818:Animals; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D004987:Ethers / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D053769:Nanocapsules / Q000737:chemistry / Q000528:radiation effects*; D010316:Particle Size; D010782:Photolysis; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D012995:Solubility
chemical_list: D000179:Acrylates; D003432:Cross-Linking Reagents; D004987:Ethers; D005452:Fluoresceins; D053769:Nanocapsules; D012710:Serum Albumin, Bovine; C028914:triethylene glycol; D011092:Polyethylene Glycols