title: In vitro bioactivity of different degree of deacetylation chitosan, a potential coating material for titanium implants.
abstract: Clinical treatment of orthopaedic tissue injuries often involves the use of titanium and titanium alloys with considerable research focusing on the surface modification of these materials. Chitosan, the partly deacetylated form of chitin, is one of the materials under investigation as surface coating for orthopaedic implants in order to improve osteo-integration and cellular attachment. In this study, we determined the effects of the degree of deacetylation (DD) of chitosan membranes on attachment, proliferation and osteogenic differentiation of MC3T3-E1 mouse preosteoblasts. Chitosan membranes were coated with fibronectin to promote biocompatibility and cellular attachment. Membranes were characterized in terms of wettability and surface topography using water contact angle measurements and atomic force microscopy. The results in this study indicate that the surface roughness and fibronectin adsorption increase with increased DD. A higher DD also facilitates attachment and proliferation of cells, but no induction of spontaneous osteogenic differentiation was observed. Lower DD chitosan membranes were successfully prepared to sustain attachment and were modified by crosslinking with glutaraldehyde to promote long-term studies. The chitosan membranes used in this study are suitable as a potential coating for titanium implants.
authors: The Blood Bank, Landspitali University Hospital, Reykjavik, Iceland.
authors: Ramona
authors: R
authors: Lieder
authors: Mariam
authors: M
authors: Darai
authors: Margrét Björk
authors: MB
authors: Thor
authors: C-H
authors: CH
authors: Ng
authors: Jón M
authors: JM
authors: Einarsson
authors: Sveinn
authors: S
authors: Gudmundsson
authors: Benedikt
authors: B
authors: Helgason
authors: Vivek Sambhaji
authors: VS
authors: Gaware
authors: Már
authors: M
authors: Másson
authors: Jóhannes
authors: J
authors: Gíslason
authors: Gissur
authors: G
authors: Orlygsson
authors: Olafur E
authors: OE
authors: Sigurjónsson
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000327:Adsorption / Q000187:drug effects; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D005353:Fibronectins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008422:Materials Testing; D051379:Mice; D018625:Microscopy, Atomic Force; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D011208:Powders; D019736:Prostheses and Implants; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D011208:Powders; D014867:Water; D048271:Chitosan; D014025:Titanium; D000469:Alkaline Phosphatase