title: Molecular characterization of Trypanosoma cruzi and infection rate of the vector Triatoma dimidiata in Costa Rica.
abstract: According to the genetic characterization by the analysis of the miniexon gene, strains of Trypanosoma cruzi can be classified into six discrete typing units (DTUs), and the DTU 1 into four distinct genotypes associated with different life cycles. While Chagas disease is endemic in Costa Rica, T. cruzi isolates from this region have never been genetically characterized. An analysis of 16 isolates from Costa Rica, based on miniexon gene analysis, showed the existence of two different haplotypes in the country, closely related to the Colombian haplotype group TcIa and to sequences from several Mexican isolates, with eight variable positions in the alignment and a variability of 2.6% between the compared sequences. No relationship between the habitat, vector or host, and the haplotypes was found, suggesting an active flow of T. cruzi in the country. The present study also reports a very high infection rate (47.3%, 26 out of 55 specimens) in a Costa Rican population of Triatoma dimidiata, the main vector of Chagas disease in this country. The distribution and abundance of the parasite and its main vector suggest a high risk of Chagas disease emergence in Costa Rica.
authors: Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain. m.angeles.zuriaga@uv.es
authors: María Ángeles
authors: MÁ
authors: Zuriaga
authors: Melissa
authors: M
authors: Blandón-Naranjo
authors: Idalia
authors: I
authors: Valerio-Campos
authors: Ruth
authors: R
authors: Salas
authors: Rodrigo
authors: R
authors: Zeledón
authors: María Dolores
authors: MD
authors: Bargues
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000469:parasitology* / Q000662:veterinary*; D003364:Costa Rica; D016054:DNA, Protozoan / Q000235:genetics; D004199:Disease Vectors; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D014224:Triatoma / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D016054:DNA, Protozoan