title: Co-cultured endometrial stromal cells and peritoneal mesothelial cells for an in vitro model of endometriosis.
abstract: This paper demonstrates an in vitro model to simulate the microenvironment of endometriosis. We used microfluidic channels with cover slips to pattern and release endometrial stromal cells (ESCs) and human peritoneal mesothelial cells (HPMCs) in a way that mimicked the pathophysiology of peritoneal endometriosis. This approach enabled observation in real time interactions between ESCs and HPMCs both in their normal and pathological states. HPMCs from control individuals were able to resist the invasion of ESCs from both control and endometriotic individuals. By contrast, HPMCs from endometriotic individuals were unable to resist the invasion of ESCs from both normal and endometriotic individuals. We further analyzed the dynamics between HPMCs and ESCs from endometriotic individuals. HPMCs from endometriotic individuals relaxed their adhesion to each other at the beginning of invasion of ESCs, lose their adhesion to the substrate and apoptosed when surrounded by ESCs. These data implicate that the peritoneal physiology may play an important role in endometriosis.
authors: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, NanoScience and NanoTechnology, Beijing, China 100190.
authors: Zhenling
authors: Z
authors: Chen
authors: Yi
authors: Y
authors: Dai
authors: Zhe
authors: Z
authors: Dong
authors: Menghui
authors: M
authors: Li
authors: Xuan
authors: X
authors: Mu
authors: Rui
authors: R
authors: Zhang
authors: Zhuo
authors: Z
authors: Wang
authors: Wei
authors: W
authors: Zhang
authors: Jinghe
authors: J
authors: Lang
authors: Jinhua
authors: J
authors: Leng
authors: Xingyu
authors: X
authors: Jiang
mesh_terms: D000328:Adult; D018920:Coculture Techniques; D004715:Endometriosis / Q000473:pathology*; D004847:Epithelial Cells / Q000166:cytology* / Q000473:pathology; D005260:Female; D006801:Humans; D044085:Microfluidics; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D008875:Middle Aged; D010532:Peritoneal Diseases / Q000473:pathology*; D017154:Stromal Cells / Q000166:cytology* / Q000473:pathology