title: Optimization of the extraction of anthocyanins from the fruit skin of Rhodomyrtus tomentosa (Ait.) Hassk and identification of anthocyanins in the extract using High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry (HPLC-ESI-MS).
abstract: Anthocyanins are naturally occurring polyphenols that impart bright color to fruits, vegetables and plants. In this study, the extraction of anthocyanins from freeze-dried fruit skin of downy rose-myrtle (Rhodomyrtus tomentosa (Ait.) Hassk var. Gangren) was optimized using response surface methodology (RSM). Using 60% ethanol containing 0.1% (v/v) hydrochloric acid as extraction solvent, the optimal conditions for maximum yields of anthocyanin (4.358 ± 0.045 mg/g) were 15.7:1 (v/w) liquid to solid ratio, 64.38 °C with a 116.88 min extraction time. The results showed good fits with the proposed model for the anthocyanin extraction (R(2) = 0.9944). Furthermore, the results of high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) analysis of the anthocyanins extracted from the fruit skin of downy rose-myrtle revealed the presence of five anthocyanin components, which were tentatively identified as delphinidin-3-glucoside, cyanidin-3-glucoside, peonidin-3-glucoside, petunidin-3-glucoside and malvidin-3-glucoside.
authors: Department of Food Science, Yingdong College of Bioengineering, Shaoguan University, Shaoguan 512005, China.|College of Food, South China Agricultural University, Guangzhou 51000, China.
authors: Guo-Ling
authors: GL
authors: Liu
authors: Department of Food Science, Yingdong College of Bioengineering, Shaoguan University, Shaoguan 512005, China.
authors: Hong-Hui
authors: 0000-0001-7837-0392
authors: HH
authors: Guo
authors: College of Food, South China Agricultural University, Guangzhou 51000, China.
authors: Yuan-Ming
authors: YM
authors: Sun
mesh_terms: D000872:Anthocyanins / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification; D027822:Myrtaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D000872:Anthocyanins; D005960:Glucosides; D010936:Plant Extracts; C462279:cyanidin-3-O-beta-glucopyranoside; C480521:peonidin 3-glucoside; C494120:delphinidin 3-O-glucopyranoside; C455107:petunidin-3-glucoside; C458419:malvidin-3-glucoside