title: MiR-23a regulates TGF-β-induced epithelial-mesenchymal transition by             targeting E-cadherin in lung cancer cells.
abstract: Transforming growth factor-β (TGF-β)-induced epithelial-mesenchymal transition             (EMT) has been shown to be related to the pathogenesis of various diseases including             lung cancer. Recently, microRNAs (miRNA) have been recognized as a new class of             genes involved in human tumorigenesis. MiR-23a/24/27a is a miRNA cluster located             in chromosome 19p13.12, which can function as an oncogene in several human cancers.             In this study, we analyzed miR-23a/24/27a expression in 10 non-small cell cancer             (NSCLC) cell lines by real-time PCR analysis. Correlation between expression of             these miRNAs and TGF-β/Smad signaling was evaluated. We found that miR-23a could             be regulated by TGF-β1 in a Smad-dependent manner in A549 lung adenocarcinoma             cells showing the EMT phenomenon. Knockdown of miR-23a partially restored E-cadherin             expression under conditions of TGF-β1 stimulation. In contrast, overexpression             of miR-23a could suppress E-cadherin expression and stimulate EMT. Furthermore,             A549 cells with overexpressed miR-23a were more resistant to gefitinib compared             to the parental cells. These findings suggest that miR-23a regulates TGF-β-induced             EMT by targeting E-cadherin in lung cancer cells and may be useful as a new therapeutic             target in NSCLC.
authors: Department of Internal Medicine, Division of Pulmonary Medicine/Infection                     and Oncology, Nippon Medical School, Tokyo 113-8603, Japan.
authors: Mengru
authors: M
authors: Cao
authors: Masahiro
authors: M
authors: Seike
authors: Chie
authors: C
authors: Soeno
authors: Hideaki
authors: H
authors: Mizutani
authors: Kazuhiro
authors: K
authors: Kitamura
authors: Yuji
authors: Y
authors: Minegishi
authors: Rintaro
authors: R
authors: Noro
authors: Akinobu
authors: A
authors: Yoshimura
authors: Li
authors: L
authors: Cai
authors: Akihiko
authors: A
authors: Gemma
mesh_terms: D015820:Cadherins / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000503:physiopathology*; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D058750:Epithelial-Mesenchymal Transition; D000077156:Gefitinib; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000503:physiopathology*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D011799:Quinazolines / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D015820:Cadherins; C552430:MIRN23a microRNA, human; D035683:MicroRNAs; D011799:Quinazolines; D034741:RNA, Small Interfering; D016212:Transforming Growth Factor beta; D000077156:Gefitinib