title: Sequence determination of a quadripartite dsRNA virus isolated from Aspergillus foetidus.
abstract: Virus infection of Aspergillus foetidus was documented over 40 years ago and was one of the first mycovirus infections described in a filamentous fungus. The virus, named Aspergillus foetidus virus (AfV), contains at least two types of icosahedral particles, called AfV-fast (-F) and AfV-slow (-S) virions, based on their relative electrophoretic mobilities. Here, we report the complete nucleotide sequence of the AfV-F genome isolated from virions purified from the prototype isolate of the fungus. The AfV-F double-stranded (ds) RNA genome is tetra-segmented, and the plus strands of each of the four segments, but not the minus strands, are polyadenylated. The organisation and sequences of the four AfV-F dsRNAs are similar to those described for Alternaria alternata virus 1, which we propose is a member of an emerging mycovirus genus ("Alternavirus") and family ("Alternaviridae"), which also includes AfV-F.
authors: Division of Cell and Molecular Biology, Faculty of Natural Sciences, Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London, SW7 2AZ, UK.
authors: Zisis
authors: Z
authors: Kozlakidis
authors: Noemi
authors: N
authors: Herrero
authors: Selin
authors: S
authors: Ozkan
authors: Lakkhana
authors: L
authors: Kanhayuwa
authors: Atif
authors: A
authors: Jamal
authors: Muhammad F
authors: MF
authors: Bhatti
authors: Robert H A
authors: RH
authors: Coutts
mesh_terms: D001230:Aspergillus / Q000821:virology*; D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D019197:Totivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins