title: Interaction of drug based copper(II) complexes with Herring Sperm DNA and their biological activities.
abstract: Square pyramidal Cu(II) complexes with NS donor ligand and ciprofloxacin have been synthesized and characterized using analytical and spectral techniques. The synthesized complexes have been tested for their antimicrobial activity using double dilution technique in terms of minimum inhibitory concentration (MIC) and colony forming unit (CFU). The DNA binding ability of the complexes with Sperm Herring DNA has been performed using absorption titration and viscosity measurement. The nuclease activity of complexes with plasmid DNA (pUC19) has been carried out using agarose gel electrophoresis technique. Synthesized complexes have been tested for their SOD mimic activity using NBT/NADH/PMS system. The cytotoxic properties of metal complexes have been evaluated using brine shrimp lethality bioassay.
authors: Department of Chemistry, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India. jeenen@gmail.com
authors: Mohan N
authors: MN
authors: Patel
authors: Chintan R
authors: CR
authors: Patel
authors: Hardik N
authors: HN
authors: Joshi
mesh_terms: D000042:Absorption; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001156:Artemia / Q000166:cytology / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects; D001681:Biological Assay; D016923:Cell Death / Q000187:drug effects; D002853:Chromatography, Liquid; D056831:Coordination Complexes / Q000378:metabolism* / Q000494:pharmacology*; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects; D004583:Electrons; D005399:Fishes / Q000378:metabolism*; D057446:Hydrodynamics; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008024:Ligands; D060328:Magnetic Phenomena; D008297:Male; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D017550:Spectroscopy, Fourier Transform Infrared; D013094:Spermatozoa / Q000378:metabolism*; D017186:Titrimetry; D014783:Viscosity / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D056831:Coordination Complexes; D008024:Ligands; D003300:Copper; D004247:DNA