title: Photoswitchable, DNA-binding helical peptides assembled with two independently designed sequences for photoregulation and DNA recognition.
abstract: Diarylethene-bridged peptides were developed to photoregulate biomolecular interactions. The peptides are made up of diarylethene-bridged and DNA-binding regions at their N- and C termini, respectively. The two regions could be independently designed and combined as desired. The α-helicities of the peptides were photoregulated in on/off or off/on manners, and the manner depended on the positions of two ornithine (Orn) residues for cross-linking reaction at the diarylethene-bridged region. In the case of the on/off manner, when the diarylethene structure adopted the open form on the peptides, the peptides folded into stable α-helices. Upon UV irradiation, the diarylethene moiety isomerized to its closed form to destabilize the helical structures. Quartz crystal microbalance (QCM) analysis showed that the open isomer strongly associated with a target DNA, as compared with the closed one. When the closed-form peptide existing in the DNA complex was irradiated with a fluorescent lamp in the middle of the QCM monitoring, the frequency change (ΔF) was enhanced by the diarylethene photoisomerization.
authors: Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kyushu Sangyo University, Matsukadai 2-3-1, Fukuoka 813-8503, Japan. kazuf@ip.kyusan-u.ac.jp
authors: Kazuhisa
authors: K
authors: Fujimoto
authors: Masaoki
authors: M
authors: Kajino
authors: Ikumi
authors: I
authors: Sakaguchi
authors: Masahiko
authors: M
authors: Inouye
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D010777:Photochemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003432:Cross-Linking Reagents; D010455:Peptides; D004247:DNA