title: Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary.
abstract: The aim of the current study was to determine metabolism of polybrominated diphenyl ether (BDE-47) in the porcine ovary. We analyzed the activity and expression of enzymes involved in phase I (CYP1A1 and CYP2B1/2) and phase II (SULT1A and COMT) of BDE-47 metabolism. Basal CYP1A1 and CYP2B1/2 activity increased during culture. BDE-47 had no effect on CYP1A1, however increased CYP2B1/2 activity after exposure for 6h. Basal SULT1A activity was 2.5 fold lower than that of COMT, and both proteins were stable during culture. BDE-47 increased SULT1A after exposure for 6 h, and COMT activity after exposure for 24 and 48 h. BDE-47 had no effect on the expression of all investigated enzymes. In conclusion, fast activation of CYP2B1/2 and late activation of COMT (with a very low basal SULT1A activity) indicates a possible action of locally produced hydroxylated metabolites prior to their detoxification.
authors: Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University, Krakow, Poland.
authors: A
authors: A
authors: Karpeta
authors: K
authors: K
authors: Warzecha
authors: J
authors: J
authors: Jerzak
authors: A
authors: A
authors: Ptak
authors: E L
authors: EL
authors: Gregoraszczuk
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D015239:Arylsulfotransferase / Q000378:metabolism*; D002394:Catechol O-Methyltransferase / Q000378:metabolism*; D002478:Cells, Cultured; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism*; D005260:Female; D055768:Halogenated Diphenyl Ethers; D010053:Ovary / Q000378:metabolism*; D011075:Polybrominated Biphenyls / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism*; D013552:Swine
chemical_list: D055768:Halogenated Diphenyl Ethers; D011075:Polybrominated Biphenyls; C511295:2,2',4,4'-tetrabromodiphenyl ether; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; D019362:Cytochrome P-450 CYP2B1; C025619:steroid 16-beta-hydroxylase; D002394:Catechol O-Methyltransferase; D015239:Arylsulfotransferase