title: Targeted single-neuron infection with rabies virus for transneuronal multisynaptic tracing.
abstract: The transynaptic and retrograde spread of rabies virus make it an efficient and robust transneuronal tracer, capable of revealing connectivity patterns of multisynaptic, neuronal circuits with great detail. Current techniques begin by infecting many neurons simultaneously, from which higher-order neurons are then labeled sequentially in time. Here we report on a method that can initially infect a single neuron-of-choice, allowing for greater precision and specificity of labeled circuits.
authors: Department of Otolaryngology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.
authors: Tuan D
authors: TD
authors: Nguyen
authors: Christoph
authors: C
authors: Wirblich
authors: Elias
authors: E
authors: Aizenman
authors: Matthias J
authors: MJ
authors: Schnell
authors: Peter L
authors: PL
authors: Strick
authors: Karl
authors: K
authors: Kandler
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004622:Embryo, Mammalian; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000821:virology*; D009924:Organ Culture Techniques; D011820:Rabies virus / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins