title: UV-modulated substrate rigidity for multiscale study of mechanoresponsive cellular behaviors.
abstract: Mechanical properties of the extracellular matrix (ECM) have profound effects on cellular functions. Here, we applied novel photosensitive polydimethylsiloxane (photoPDMS) chemistry to create photosensitive, biocompatible photoPDMS as a rigidity-tunable material for study of mechanoresponsive cellular behaviors. By modulating the PDMS cross-linker to monomer ratio, UV light exposure time, and postexposure baking time, we achieved a broad range of bulk Young's modulus for photoPDMS from 0.027 to 2.48 MPa. Biocompatibility of photoPDMS was assayed, and no significant cytotoxic effect was detected as compared to conventional PDMS. We demonstrated that the bulk Young's modulus of photoPDMS could impact cell morphology, adhesion formation, cytoskeletal structure, and cell proliferation. We further fabricated photoPDMS micropost arrays for multiscale study of mechanoresponsive cellular behaviors. Our results suggested that adherent cells could sense and respond to changes of substrate rigidity at a subfocal adhesion resolution. Together, we demonstrated the potential of photoPDMS as a photosensitive and rigidity-tunable material for mechanobiology studies.
authors: Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, Michigan 48109, United States.
authors: Yubing
authors: Y
authors: Sun
authors: Liang-Ting
authors: LT
authors: Jiang
authors: Ryoji
authors: R
authors: Okada
authors: Jianping
authors: J
authors: Fu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D017319:Photosensitizing Agents / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D017319:Photosensitizing Agents; C013830:baysilon