title: Erythrocyte-bound immunoglobulin isotypes in dogs with immune-mediated hemolytic anemia: 54 cases (2001-2010).
abstract: OBJECTIVE
  To identify erythrocyte-bound immunoglobulin (Ig) isotypes in dogs with primary immune-mediated hemolytic anemia (IMHA).
  
  
  METHODS
  Retrospective case series. Animals-54 dogs with IMHA.
  
  
  METHODS
  Medical records of dogs with IMHA diagnosed between January 2001 and April 2010 were examined. Immunoglobulin isotype (tested via direct immunofluorescence by flow cytometry to identify erythrocyte-bound Ig), Hct, serum bilirubin concentration, presence of autoagglutination, degree of spherocytosis, duration of hospitalization, and 90-day outcome were recorded.
  
  
  RESULTS
  The Hct on admission was significantly lower in dogs with IgG and IgM isotypes bound to erythrocytes, compared with dogs with a single Ig isotype, and the degree of spherocytosis was greater in dogs with IgG and IgM bound to erythrocytes, compared with dogs that only had IgM. Dogs with only IgM were not more likely to have autoagglutination, compared with dogs that only had IgG on the erythrocyte surface. Although Ig isotype was not associated with survival time, initial serum total bilirubin concentration was higher in nonsurvivors.
  
  
  CONCLUSIONS
  Results suggested that dogs with IMHA with ≥ 2 Ig isotypes bound to erythrocytes, particularly IgG and IgM, are likely to have a more severe degree of anemia, spherocytosis, and autoagglutination.
authors: Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA. harkin@vet.k-state.edu
authors: Kenneth R
authors: KR
authors: Harkin
authors: Jill A
authors: JA
authors: Hicks
authors: Melinda J
authors: MJ
authors: Wilkerson
mesh_terms: D000743:Anemia, Hemolytic / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D007136:Immunoglobulins / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D008297:Male; D012189:Retrospective Studies
chemical_list: D007136:Immunoglobulins