title: H1N1/09 influenza A virus infection of immortalized first trimester human trophoblast cell lines.
abstract: OBJECTIVE
  Although high maternal mortality was reported in the most recent pandemic of swine-origin influenza A H1N1/09 (H1N1/09), its direct effects on the feto-placental unit are unknown. In the present study, we examined the susceptibility of immortalized human trophoblasts to clinical isolates of pandemic 2009 H1N1 influenza A (H1N1/09) virus.
  
  
  METHODS
  The H1N1/09 virus was isolated from a patient with influenza, sequenced and identified as the A/Narita/2009 (H1N1) strain. The trophoblast cell lines Swan71 and HTR8 were challenged with the virus and examined for the expression of H1N1/09 viral RNA and proteins.
  
  
  RESULTS
  Viral RNA and proteins were observed 24 hr after inoculation. However, viral release was not detected.
  
  
  CONCLUSIONS
  First trimester human trophoblast cell lines were susceptible to the H1N1/09 influenza A virus. However, viral release and cytopathic effects were minimal. Our data suggest that placental damage by the H1N1/09 virus may be limited.
authors: Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan. aizawa.shihoko@nihon-u.ac.jp
authors: Shihoko
authors: S
authors: Komine-Aizawa
authors: Ai
authors: A
authors: Suzaki
authors: Quang D
authors: QD
authors: Trinh
authors: Yasuyuki
authors: Y
authors: Izumi
authors: Toshikatsu
authors: T
authors: Shibata
authors: Kazumichi
authors: K
authors: Kuroda
authors: Satoshi
authors: S
authors: Hayakawa
mesh_terms: D002461:Cell Line, Transformed; D005260:Female; D054884:Host-Pathogen Interactions; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000254:growth & development / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000821:virology; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012367:RNA, Viral / Q000096:biosynthesis* / Q000276:immunology; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology* / Q000821:virology; D014779:Virus Replication / Q000276:immunology
chemical_list: D012367:RNA, Viral