title: Intravenous administration of human umbilical cord blood-mononuclear cells dose-dependently relieve neurologic deficits in rat intracerebral hemorrhage model.
abstract: Human umbilical cord blood (HUCB) is now considered as a valuable source for stem cell-based therapies. Previous studies showed that intravascular injection of the HUCB significantly improves neurological functional recovery in a model of intracerebral hemorrhage (ICH). To extend these findings, we examined the behavioral recovery and injured volume in the presence of increasing doses of human umbilical cord blood derived mononuclear cells (HUC-MCs) after intracerebral hemorrhage in rats. The experimental ICH was induced by intrastriatal administration of bacterial collagenase IV in adult rats. One day after the surgery, the rats were randomly divided into 4 groups to receive intravenously either BrdU positive human UC-MCs (4 × 10(6), 8 × 10(6) and 16 × 10(6) cells in 1 ml saline, n=10, respectively) as treated groups or the same amount of saline as lesion group (n=10). There was also one group (control n=10) that received only the vehicle solution of collagenase. The animals were evaluated for 14 days with modified limb placing and corner turn tests. The transplanted human UC-MCs were also detected by immunohistochemistry with labeling of BrdU. Two weeks after infusion, there was a significant recovery in the behavioral performance when 4 × 10(6) or more UC-MCs were delivered (P<0.05-0.001). Injured volume measurements disclosed an inverse relationship between UC-MCs dose and damage reaching significance at the higher UC-MCs doses. Moreover, human UC-MCs were localized by immunohistochemistry only in the injured area. Intravenously transplanted UC-MCs can accelerate the neurological function recovery of ICH rat and diminish the striatum lesion size by demonstrating a dose relationship between them.
authors: Department of Anatomy & Cell Biology, Medical Faculty, Mashhad University of Medical Sciences, Khorasan Razavi, Iran.
authors: Masoumeh
authors: M
authors: Seghatoleslam
authors: Mehdi
authors: M
authors: Jalali
authors: Mohammad Reza
authors: MR
authors: Nikravesh
authors: Daryoush
authors: D
authors: Hamidi Alamdari
authors: Mahmoud
authors: M
authors: Hosseini
authors: Alireza
authors: A
authors: Fazel
mesh_terms: D000818:Animals; D000963:Antimetabolites; D001522:Behavior, Animal / Q000502:physiology; D001921:Brain / Q000473:pathology; D001973:Bromodeoxyuridine; D002465:Cell Movement; D002469:Cell Separation; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D020300:Intracranial Hemorrhages / Q000150:complications* / Q000473:pathology / Q000628:therapy*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000637:transplantation*; D009422:Nervous System Diseases / Q000209:etiology* / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D020521:Stroke / Q000209:etiology / Q000628:therapy
chemical_list: D000963:Antimetabolites; D017493:Leukocyte Common Antigens; D001973:Bromodeoxyuridine