title: Seroprevalence of Lyme disease and genospecies of Borrelia burgdorferi sensu lato in patients diagnosed with borreliosis in the Province of Warmia-Masuria in north-eastern Poland.
abstract: Between 2009-2010, a group of 259 patients suspected of contracting Lyme disease were examined in the Provincial Sanitary-Epidemiological Station in Olsztyn for the presence of IgM and IgG against specific Borrelia burgdorferi sensu lato (s.l.) genospecies antigens by immunoblot. A total of 27.4% and 29.0% of the blood serum samples showed positive and uncertain results for IgM and IgG antibodies. The majority of positive and uncertain results were found in patients aged 30-40 years (30%) for IgM, and people aged 50 and over (35.8%) for IgG. Significantly more positive results for IgG were found in males (40.2%) then females (19.7%). In both groups, similar proportions of positive results for IgM anti-Borrelia were recorded (26.1% of females and 29% of males. In 71.5% of patients, IgM against flagellin protein (p41) of B. burgdorferi sensu stricto (s.s.) was found. For IgG, the most frequently detected antibodies were found against the p41 protein of B. burgdorferi s.s. (64.8%) and the recombinant variable surface antigens (VlsE) (49%). Among all the analysed antigens those of B. burgdorferi s.s. were the most frequent cause of immunological reaction, followed by antigens of B. afzelii and B. garinii. Reaction to antigens of B. spielmanii was rarely detected.
authors: Department of Medical Biology, University of Warmia and Mazury, Olsztyn, Poland. katarzyna.kubiak@uwm.edu.pl
authors: Katarzyna
authors: K
authors: Kubiak
authors: Ewa
authors: E
authors: Dzika
authors: Joanna
authors: J
authors: Równiak
authors: Małgorzata
authors: M
authors: Dziedziech
authors: Janusz
authors: J
authors: Dzisko
mesh_terms: D000328:Adult; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D000954:Antigens, Surface / Q000276:immunology; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002675:Child, Preschool; D005260:Female; D005408:Flagellin / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D008193:Lyme Disease / Q000097:blood / Q000453:epidemiology / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D011044:Poland / Q000453:epidemiology; D015995:Prevalence; D016036:Seroepidemiologic Studies; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D000954:Antigens, Surface; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005408:Flagellin