title: Litorilinea aerophila gen. nov., sp. nov., an aerobic member of the class Caldilineae, phylum Chloroflexi, isolated from an intertidal hot spring.
abstract: A thermophilic, aerobic, Gram-stain-negative, filamentous bacterium, strain PRI-4131(T), was isolated from an intertidal hot spring in Isafjardardjup, NW Iceland. The strain grew chemo-organotrophically on various carbohydrates. The temperature range for growth was 40-65 °C (optimum 55 °C), the pH range was pH 6.5-9.0 (optimum pH 7.0) and the NaCl range was 0-3 % (w/v) (optimum 0.5 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PRI-4131(T) represented a distinct lineage within the class Caldilineae of the phylum http://dx.doi.org/10.1601/nm.550Chloroflexi. The highest levels of sequence similarity, about 91 %, were with Caldilinea aerophila STL-6-O1(T) and Caldilinea tarbellica D1-25-10-4(T). Fermentative growth was not observed for strain PRI-4131(T), which, in addition to other characteristics, distinguished it from the two Caldilinea species. Owing to both phylogenetic and phenotypic differences from the described members of the class Caldilineae, we propose to accommodate strain PRI-4131(T) in a novel species in a new genus, Litorilinea aerophila gen. nov., sp. nov. The type strain of Litorilinea aerophila is PRI-4131(T) ( = DSM 25763(T)  = ATCC BAA-2444(T)).
authors: Faculty of Pharmaceutical Sciences, University of Iceland, Hagi, Hofsvallagata 53, IS-101 Reykjavík, Iceland.|Matís, Vínlandsleið 12, IS-113 Reykjavík, Iceland.
authors: Varsha
authors: V
authors: Kale
authors: Matís, Vínlandsleið 12, IS-113 Reykjavík, Iceland.
authors: Snædís H
authors: SH
authors: Björnsdóttir
authors: Matís, Vínlandsleið 12, IS-113 Reykjavík, Iceland.
authors: Ólafur H
authors: ÓH
authors: Friðjónsson
authors: Matís, Vínlandsleið 12, IS-113 Reykjavík, Iceland.
authors: Sólveig K
authors: SK
authors: Pétursdóttir
authors: Faculty of Pharmaceutical Sciences, University of Iceland, Hagi, Hofsvallagata 53, IS-101 Reykjavík, Iceland.
authors: Sesselja
authors: S
authors: Ómarsdóttir
authors: Matís, Vínlandsleið 12, IS-113 Reykjavík, Iceland.
authors: Guðmundur Óli
authors: GÓ
authors: Hreggviðsson
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D041862:Chloroflexi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D045482:Hot Springs / Q000382:microbiology*; D007055:Iceland; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S