title: High-throughput assessment of microRNA activity and function using microRNA sensor and decoy libraries.
abstract: We introduce two large-scale resources for functional analysis of microRNA (miRNA): a decoy library for inhibiting miRNA function and a sensor library for monitoring microRNA activity. To take advantage of the sensor library, we developed a high-throughput assay called Sensor-seq to simultaneously quantify the activity of hundreds of miRNAs. Using this approach, we show that only the most abundant miRNAs in a cell mediate target suppression. Over 60% of detected miRNAs had no discernible activity, which indicated that the functional 'miRNome' of a cell is considerably smaller than currently inferred from profiling studies. Moreover, some highly expressed miRNAs exhibited relatively weak activity, which in some cases correlated with a high target-to-miRNA ratio or increased nuclear localization of the miRNA. Finally, we show that the miRNA decoy library can be used for pooled loss-of-function studies. These tools are valuable resources for studying miRNA biology and for miRNA-based therapeutics.
authors: Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, New York, USA.
authors: Gavriel
authors: G
authors: Mullokandov
authors: Alessia
authors: A
authors: Baccarini
authors: Albert
authors: A
authors: Ruzo
authors: Anitha D
authors: AD
authors: Jayaprakash
authors: Navpreet
authors: N
authors: Tung
authors: Benjamin
authors: B
authors: Israelow
authors: Matthew J
authors: MJ
authors: Evans
authors: Ravi
authors: R
authors: Sachidanandam
authors: Brian D
authors: BD
authors: Brown
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics*; D057166:High-Throughput Screening Assays; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D035683:MicroRNAs