title: Human mesenchymal stem cells derived from limb bud can differentiate into all three embryonic germ layers lineages.
abstract: Mesenchymal stem cells (MSCs) have been isolated from many sources, including adults and fetuses. Previous studies have demonstrated that, compared with their adult counterpart, fetal MSCs with several remarkable advantages may be a better resource for clinical applications. In this study, we successfully isolated a rapidly proliferating cell population from limb bud of aborted fetus and termed them "human limb bud-derived mesenchymal stem cells" (hLB-MSCs). Characteristics of their morphology, phenotype, cell cycle, and differentiation properties were analyzed. These adherent cell populations have a typically spindle-shaped morphology. Flow cytometry analysis showed that hLB-MSCs are positive for CD13, CD29, CD90, CD105, and CD106, but negative for CD3, CD4, CD5, CD11b, CD14, CD15, CD34, CD45, CD45RA, and HLA-DR. The detection of cell cycle from different passages indicated that hLB-MSCs have a similar potential for propagation during long culture in vitro. The most novel finding here is that, in addition to their mesodermal differentiation (osteoblasts and adipocytes), hLB-MSCs can also differentiated into extramesenchymal lineages, such as neural (ectoderm) and hepatic (endoderm) progenies. These results indicate that hLB-MSCs have a high level of plasticity and can differentiate into cell lineages from all three embryonic layers in vitro.
authors: Department of Biochemistry and Molecular Biology, Binzhou Medical College, Yantai, Shandong Province, People's Republic of China.
authors: Fei
authors: F
authors: Jiao
authors: Juan
authors: J
authors: Wang
authors: Zhao-Lun
authors: ZL
authors: Dong
authors: Min-Juan
authors: MJ
authors: Wu
authors: Ting-Bao
authors: TB
authors: Zhao
authors: Dan-Dan
authors: DD
authors: Li
authors: Xin
authors: X
authors: Wang
mesh_terms: D037881:Aborted Fetus / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D002454:Cell Differentiation; D005855:Germ Layers / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D018878:Limb Buds / Q000166:cytology* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation