title: Comparison of the effects of Mylabris and Acanthopanax senticosus on promising cancer marker polyamines in plasma of a Hepatoma-22 mouse model using HPLC-ESI-MS.
abstract: A simple and sensitive method for the simultaneous determination of plasma concentrations of five polyamines in normal and Hepatoma-22 mice, and mice treated with Mylabris and Acanthopanax senticosus was developed by HPLC-ESI-MS. Male Kunming mice were divided into nine groups, a control group (inoculation without treatment), a positive group (Cyclophosphamide), treatment groups [Mylabris (4, 8, 16 mg/kg), Acanthopanax senticosus (6, 12, 24 g/kg)] and a normal group (without inoculation). Twenty-four hours after the last administration, plasma samples were collected. The derived polyamines were separated on a C(18) column by a gradient elution using methanol-water with excellent linearity within the range from 2.5 to 1000 ng/mL. Polyamines were confirmed as useful biochemical markers of hepatoma. The differences in anti-cancer therapeutic efficacy between Mylabris and Acanthopanax senticosus might contribute to the variability of polyamine levels in vivo. This HPLC-ESI-MS method was successfully applied to investigate the relationship between polyamines and cancer in mice and might be a useful method to test the activity of potential anti-tumor drugs.
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
authors: Qian
authors: Q
authors: Wang
authors: Yixiang
authors: Y
authors: Wang
authors: Ran
authors: R
authors: Liu
authors: Xu
authors: X
authors: Yan
authors: Yujiao
authors: Y
authors: Li
authors: Hui
authors: H
authors: Fu
authors: Kaishun
authors: K
authors: Bi
authors: Qing
authors: Q
authors: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000097:blood* / Q000737:chemistry; D001835:Body Weight / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D001517:Coleoptera / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D028001:Eleutherococcus / Q000737:chemistry*; D008114:Liver Neoplasms, Experimental / Q000097:blood* / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D011073:Polyamines / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011073:Polyamines