title: An aryleneethynylene fluorophore for cell membrane staining.
abstract: The use of an amphiphilic aryleneethynylene fluorophore as a plasma membrane marker in fixed and living mammalian cells and liposome model systems is demonstrated. We show here that the optical properties of the novel dye are almost independent on pH, in the range 5.0-8.0. Spectroscopic characterization performed on unilamellar liposomes ascertained that the fluorescence intensity of the aryleneethynylene fluorophore greatly increases after incorporation in lipidic membranes. Experiments performed on different mammalian cells demonstrated that the novel membrane marker exhibits fast staining and a good photostability that make it a suitable tool for live cell imaging. Importantly, the aryleneethynylene fluorophore was also shown to be a fast and reliable blue membrane marker in classical multicolor immunofluorescence experiments. This study adds new important findings to the recent exploitation of the wide class of aryleneethynylene molecules as luminescent markers for biological investigations.
authors: Consiglio Nazionale delle Ricerche, Istituto di Chimica dei Composti Organo Metallici, Bari, Italy.
authors: Antonio
authors: A
authors: Cardone
authors: Francesco
authors: F
authors: Lopez
authors: Francesco
authors: F
authors: Affortunato
authors: Giovanni
authors: G
authors: Busco
authors: Aldebaran M
authors: AM
authors: Hofer
authors: Rosanna
authors: R
authors: Mallamaci
authors: Carmela
authors: C
authors: Martinelli
authors: Matilde
authors: M
authors: Colella
authors: Gianluca M
authors: GM
authors: Farinola
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D006224:Cricetinae; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence
chemical_list: D005456:Fluorescent Dyes