title: Analysis of histone deacetylase inhibitor-induced responses in human periodontal ligament fibroblasts.
abstract: Periodontal ligament (PDL) fibroblasts play critical roles in the regeneration of periodontal tissues damaged by periodontitis. Histone deacetylase inhibitors (HDIs) have been suggested to be potential tools in tissue engineering. The feasibility of using the HDI, sodium butyrate (NaB) for periodontal regeneration was examined by evaluating its effect on the osteogenic differentiation of human PDL fibroblasts and its modulation of the inflammatory responses to lipopolysaccharide (LPS). NaB did not cause significant cell death at 100 μM but promoted the expression of the osteoblast phenotype (Runx2, osterix, osteocalcin, and bone sialoprotein). NaB significantly inhibited the LPS-induced production of reactive oxygen species and the expression of pro-inflammatory cytokines (IL-1β and TNF-α). These results suggest that HDIs can offer a potential therapeutic agent for periodontal regeneration.
authors: Department of Periodontology, School of Dentistry, Seoul National University, Seoul, Republic of Korea. periopf@snu.ac.kr
authors: Tae-Il
authors: TI
authors: Kim
authors: Ji-Eun
authors: JE
authors: Han
authors: Hong-Moon
authors: HM
authors: Jung
authors: Joung-Hwan
authors: JH
authors: Oh
authors: Kyung Mi
authors: KM
authors: Woo
mesh_terms: D002087:Butyrates / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D002087:Butyrates; D016207:Cytokines; D056572:Histone Deacetylase Inhibitors; D017382:Reactive Oxygen Species