title: Winding single-molecule double-stranded DNA on a nanometer-sized reel.
abstract: A molecular system of a nanometer-sized reel was developed from F(1)-ATPase, a rotary motor protein. By combination with magnetic tweezers and optical tweezers, single-molecule double-stranded DNA (dsDNA) was wound around the molecular reel. The bending stiffness of dsDNA was determined from the winding tension (0.9-6.0 pN) and the diameter of the wound loop (21.4-8.5 nm). Our results were in good agreement with the conventional worm-like chain model and a persistence length of 54 ± 9 nm was estimated. This molecular reel system offers a new platform for single-molecule study of micromechanics of sharply bent DNA molecules and is expected to be applicable to the elucidation of the molecular mechanism of DNA-associating proteins on sharply bent DNA strands.
authors: Department of Applied Chemistry, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors: Huijuan
authors: H
authors: You
authors: Ryota
authors: R
authors: Iino
authors: Rikiya
authors: R
authors: Watanabe
authors: Hiroyuki
authors: H
authors: Noji
mesh_terms: D004247:DNA / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D052898:Optical Tweezers; D006180:Proton-Translocating ATPases / Q000737:chemistry
chemical_list: D004247:DNA; D006180:Proton-Translocating ATPases