title: IGFBP7's susceptibility to proteolysis is altered by A-to-I RNA editing of its transcript.
abstract: The selective deamination of adenosines (A) to inosines (I) in messenger RNAs (mRNAs) can alter the encoded protein's amino acid sequence, with often critical consequences on protein stability, localization, and/or function. Insulin-like growth factor-binding protein 7 (IGFBP7) supports cell-adhesion and stimulates fibroblast proliferation with IGF and insulin. It exists in both proteolytically processed and unprocessed forms with altered cell-extracellular matrix interactions. Here we show that editing of IGFBP7 transcripts impacts the protein's susceptibility to proteolytic cleavage, thus providing a means for a cell to modulate its functionality through A-to-I RNA editing.
authors: Department of Biological Sciences, Lehigh University, Bethlehem, PA 18015, USA. cps206@lehigh.edu
authors: Christina
authors: C
authors: Godfried Sie
authors: Stephen
authors: S
authors: Hesler
authors: Stefan
authors: S
authors: Maas
authors: Michael
authors: M
authors: Kuchka
mesh_terms: D000241:Adenosine / Q000737:chemistry; D000243:Adenosine Deaminase / Q000378:metabolism*; D001483:Base Sequence; D002448:Cell Adhesion; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007288:Inosine / Q000737:chemistry; D018969:Insulin-Like Growth Factor Binding Proteins / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D025941:Protein Interaction Mapping; D020033:Protein Isoforms; D059748:Proteolysis; D017393:RNA Editing; D016601:RNA-Binding Proteins; D012697:Serine Endopeptidases / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D018969:Insulin-Like Growth Factor Binding Proteins; D020033:Protein Isoforms; D016601:RNA-Binding Proteins; C102529:insulin-like growth factor binding protein-related protein 1; D007288:Inosine; D007334:Insulin-Like Growth Factor I; D012697:Serine Endopeptidases; C485596:ST14 protein, human; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase; D000241:Adenosine