title: Expression changes of hypothalamic Ahi1 in mice brain: implication in sensing insulin signaling.
abstract: Growing evidence suggests that the brain, in particular the hypothalamus, directly senses hormones and nutrients to initiate feeding behavior and metabolic responses in the control of energy homeostasis. However, the molecular mechanisms underlying this important process have remained largely unknown. Our study provides the evidence for the role of Abelson helper integration site 1 (Ahi1) protein as a sensor of insulin signaling in the hypothalamus. We found that fasting increased the expression of hypothalamic Ahi1 which was accompanied by lower levels of circulating insulin compared with satiated mice, while re-feeding decreased the expression of hypothalamic Ahi1 which was accompanied by higher levels of circulating insulin. We also found the up-regulated expression of hypothalamic Ahi1 in high-fat induced obese mice, db/db mice, and streptozotocin induced diabetic mice. In addition, we demonstrated that insulin could decrease the expression of Ahi1 in neuroblastoma cell line N18TG2. Taken together, our results indicate that hypothalamic Ahi1 functions as a sensor of insulin signaling.
authors: Department of Endocrinology, Lin Yi People's Hospital, No 27 Jiefang Road, Linyi 276003, People's Republic of China.
authors: Shaona
authors: S
authors: Niu
authors: Hao
authors: H
authors: Wang
authors: Zhenbo
authors: Z
authors: Huang
authors: Xiurong
authors: X
authors: Rao
authors: Xiangsheng
authors: X
authors: Cai
authors: Tao
authors: T
authors: Liang
authors: Jing
authors: J
authors: Xu
authors: Xingshun
authors: X
authors: Xu
authors: Guoqing
authors: G
authors: Sheng
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000502:physiology; D002460:Cell Line; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D005508:Food Deprivation; D015870:Gene Expression; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000378:metabolism* / Q000502:physiology; D007328:Insulin / Q000097:blood / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000378:metabolism; D009928:Organ Specificity; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013311:Streptozocin
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C465818:Ahi1 protein, mouse; D007328:Insulin; D011518:Proto-Oncogene Proteins; D013311:Streptozocin