title: Fluorescence correlation spectroscopy to determine the diffusion coefficient of α-synuclein and follow early oligomer formation.
abstract: Fluorescence correlation spectroscopy (FCS) can be used to determine the diffusion coefficient of fluorescently labeled α-synuclein. It is a technique based on the use of a confocal microscope. By applying FCS in a combination of short sampling times and repeated measurements, the disappearance of individual α-synuclein molecules (called monomers) and the formation of oligomers can be characterized during the early aggregation process.
authors: Laboratory of Biomolecular Dynamics, University of Leuven, Leuven, Belgium.
authors: Sangeeta
authors: S
authors: Nath
authors: Manli
authors: M
authors: Deng
authors: Yves
authors: Y
authors: Engelborghs
mesh_terms: D000465:Algorithms; D019943:Amino Acid Substitution; D004058:Diffusion; D005456:Fluorescent Dyes / Q000737:chemistry; D007700:Kinetics; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D051844:alpha-Synuclein / Q000737:chemistry* / Q000235:genetics
chemical_list: D005456:Fluorescent Dyes; D051844:alpha-Synuclein