title: Proteomic analysis of α-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor complexes.
abstract: The AMPA receptor (AMPA-R) is a major excitatory neurotransmitter receptor in the brain. Identifying and characterizing the neuronal proteins interacting with AMPA-Rs have provided important information about the molecular mechanisms underlying synaptic transmission and plasticity. In this study, to identify more AMPA-R interactors in vivo, we performed proteomic analyses of AMPA-R complexes from the brain. AMPA-R complexes were isolated from the brain through various combinations of biochemical techniques for solubilization, enrichment, and immunoprecipitation. Mass spectrometry analyses of these isolated complexes identified several novel components of the AMPA-R complexes as well as some previously identified components. The identification of these novel components helps to further define the complex mechanisms involved in the regulation of AMPA receptor function and synaptic plasticity.
authors: Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, Texas 77555, USA. mykang@utmb.edu
authors: Myoung-Goo
authors: MG
authors: Kang
authors: Mutsuo
authors: M
authors: Nuriya
authors: Yurong
authors: Y
authors: Guo
authors: Kevin D
authors: KD
authors: Martindale
authors: Daniel Z
authors: DZ
authors: Lee
authors: Richard L
authors: RL
authors: Huganir
mesh_terms: D000818:Animals; D046912:Multiprotein Complexes / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D018091:Receptors, AMPA / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D046912:Multiprotein Complexes; D018091:Receptors, AMPA