title: Human cardiosphere-derived cells from patients with chronic ischaemic heart disease can be routinely expanded from atrial but not epicardial ventricular biopsies.
abstract: To investigate the effects of age and disease on endogenous cardiac progenitor cells, we obtained right atrial and left ventricular epicardial biopsies from patients (n = 22) with chronic ischaemic heart disease and measured doubling time and surface marker expression in explant- and cardiosphere-derived cells (EDCs, CDCs). EDCs could be expanded from all atrial biopsy samples, but sufficient cells for cardiosphere culture were obtained from only 8 of 22 ventricular biopsies. EDCs from both atrium and ventricle contained a higher proportion of c-kit+ cells than CDCs, which contained few such cells. There was wide variation in expression of CD90 (atrial CDCs 5-92 % CD90+; ventricular CDCs 11-89 % CD90+), with atrial CDCs cultured from diabetic patients (n = 4) containing 1.6-fold more CD90+ cells than those from non-diabetic patients (n = 18). No effect of age or other co-morbidities was detected. Thus, CDCs from atrial biopsies may vary in their therapeutic potential.
authors: Department of Physiology, Anatomy and Genetics, University of Oxford, Sherrington Building, Parks Road, Oxford, UK.
authors: Helen H L
authors: HH
authors: Chan
authors: Zaal
authors: Z
authors: Meher Homji
authors: Renata S M
authors: RS
authors: Gomes
authors: Dominic
authors: D
authors: Sweeney
authors: George N
authors: GN
authors: Thomas
authors: Jun Jie
authors: JJ
authors: Tan
authors: Huajun
authors: H
authors: Zhang
authors: Filippo
authors: F
authors: Perbellini
authors: Daniel J
authors: DJ
authors: Stuckey
authors: Suzanne M
authors: SM
authors: Watt
authors: David
authors: D
authors: Taggart
authors: Kieran
authors: K
authors: Clarke
authors: Enca
authors: E
authors: Martin-Rendon
authors: Carolyn A
authors: CA
authors: Carr
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D002908:Chronic Disease; D003920:Diabetes Mellitus / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006325:Heart Atria / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006352:Heart Ventricles / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D006937:Hypercholesterolemia / Q000473:pathology; D006973:Hypertension / Q000473:pathology; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D010496:Pericardium / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D012720:Severity of Illness Index; D012907:Smoking / Q000473:pathology; D018874:Spheroids, Cellular; D013234:Stem Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D018800:Thy-1 Antigens / Q000378:metabolism
chemical_list: D015415:Biomarkers; D018800:Thy-1 Antigens; D019009:Proto-Oncogene Proteins c-kit