title: Protein expression pattern in response to ionizing radiation in MCF-7 human breast cancer cells.
abstract: Breast cancer is one of the most common types of cancer in women and is highly treatable by radiotherapy. However, repeated exposure to radiation results in tumor cell resistance. Understanding the molecular mechanisms involved in the response of tumors to γ-irradiation is important for improving radiotherapy. For this reason, we aimed to identify radiation-responsive genes at the protein level. In the present study, we observed differentially expressed proteins using 2D-PAGE and MALDI-TOF-MS for the global analysis of protein expression patterns in response to ionizing radiation (IR). When the expression patterns of proteins were compared to a control gel, numerous spots were found that differed greatly. Among them, 11 spots were found to be significantly different. One set of proteins (GH2, RGS17, BAK1, CCNH, TSG6, RAD51B, IGFBP1 and CASP14) was upregulated and another set of proteins (C1QRF, PLSCR2 and p34(SE1-1)) was downregulated after exposure to γ-rays. These proteins are known to be related to cell cycle control, apoptosis, DNA repair, cell proliferation and other signaling pathways.
authors: Research Center for Women's Diseases, Sookmyung Women's University, Seoul.
authors: Samil
authors: S
authors: Jung
authors: Soonduck
authors: S
authors: Lee
authors: Jayhee
authors: J
authors: Lee
authors: Chengping
authors: C
authors: Li
authors: Ji-Yeon
authors: JY
authors: Ohk
authors: Hyeon-Kyung
authors: HK
authors: Jeong
authors: Seungkyu
authors: S
authors: Lee
authors: Sangwoo
authors: S
authors: Kim
authors: Yunyeong
authors: Y
authors: Choi
authors: Sunghak
authors: S
authors: Kim
authors: Heungwoo
authors: H
authors: Lee
authors: Myeong-Sok
authors: MS
authors: Lee