title: Immunization of olive flounder (Paralichthys olivaceus) with an auxotrophic Edwardsiella tarda mutant harboring the VHSV DNA vaccine.
abstract: The aims of the present study were to find more powerful promoter for DNA vaccines in olive flounder (Paralichthys olivaceus) and to evaluate the availability of the auxotrophic Edwardsiella tarda mutant (Δalr Δasd E. tarda) as a delivery vehicle for DNA vaccine against VHSV in olive flounder. The marine medaka (Oryzias dancena) β-actin promoter was clearly stronger than cytomegalovirus (CMV) promoter when the vectors were transfected to Epithelioma papulosum cyprini (EPC) cells or injected into the muscle of olive flounder, suggesting that marine medaka β-actin promoter would be more appropriate promoter for DNA vaccines in olive flounder than CMV promoter. Olive flounder immunized with the Δalr Δasd E. tarda harboring viral hemorrhagic septicemia virus (VHSV) DNA vaccine vector driven by the marine medaka β-actin promoter showed significantly higher serum neutralization titer and higher survival rates against challenge with VHSV than fish immunized with the bacteria carrying VHSV DNA vaccine vector driven by CMV promoter. These results indicate that auxotrophic E. tarda mutant harboring marine medaka β-actin promoter-driven DNA vaccine vectors would be a potential system for prophylactics of infectious diseases in olive flounder.
authors: Department of Aquatic Life Medicine, Pukyong National University, 599-1, Daeyeondong, Namgu, Busan 608-737, Republic of Korea.
authors: Seung Hyuk
authors: SH
authors: Choi
authors: Min Sun
authors: MS
authors: Kim
authors: Ki Hong
authors: KH
authors: Kim
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics; D020610:Edwardsiella ictaluri / Q000235:genetics / Q000276:immunology; D005393:Fish Diseases / Q000517:prevention & control*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005432:Flounder / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D031941:Hemorrhagic Septicemia, Viral / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D007273:Injections, Intramuscular / Q000662:veterinary; D008164:Luminescent Proteins / Q000378:metabolism; D029141:Novirhabdovirus / Q000235:genetics / Q000276:immunology*; D009990:Oryzias / Q000235:genetics; D011401:Promoter Regions, Genetic; D056910:Republic of Korea; D017778:Vaccines, Combined / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology* / Q000821:virology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D000097573:Red Fluorescent Protein
chemical_list: D000199:Actins; D001428:Bacterial Vaccines; D029941:Fish Proteins; D008164:Luminescent Proteins; D017778:Vaccines, Combined; D019444:Vaccines, DNA; D014765:Viral Vaccines; D000097573:Red Fluorescent Protein