title: Classification of the four main types of lung cancer using a microRNA-based diagnostic assay.
abstract: For patients with primary lung cancer, accurate determination of the tumor type significantly influences treatment decisions. However, techniques and methods for lung cancer typing lack standardization. In particular, owing to limited tumor sample amounts and the poor quality of some samples, the classification of primary lung cancers using small preoperative biopsy specimens presents a diagnostic challenge using current tools. We previously described a microRNA-based assay (miRview squamous; Rosetta Genomics Ltd., Rehovot, Israel) that accurately differentiates between squamous and nonsquamous non-small cell lung cancer. Herein, we describe the development and validation of an assay that differentiates between the four main types of lung cancer: squamous cell carcinoma, nonsquamous non-small cell lung cancer, carcinoid, and small cell carcinoma. The assay, miRview lung (Rosetta Genomics Ltd.), is based on the expression levels of eight microRNAs, measured using a sensitive quantitative RT-PCR platform. It was validated on an independent set of 451 samples, more than half of which were preoperative cytologic samples (fine-needle aspiration and bronchial brushing and washing). The assay returned a result for more than 90% of the samples with overall accuracy of 94% (95% CI, 91% to 96%), with similar performance observed in pathologic and cytologic samples. Thus, miRview lung is a simple and reliable diagnostic assay that offers an accurate and standardized classification tool for primary lung cancer using pathologic and cytologic samples.
authors: Rosetta Genomics, Rehovot, Israel.
authors: Shlomit
authors: S
authors: Gilad
authors: Gila
authors: G
authors: Lithwick-Yanai
authors: Iris
authors: I
authors: Barshack
authors: Sima
authors: S
authors: Benjamin
authors: Irit
authors: I
authors: Krivitsky
authors: Tina Bocker
authors: TB
authors: Edmonston
authors: Marluce
authors: M
authors: Bibbo
authors: Craig
authors: C
authors: Thurm
authors: Laurie
authors: L
authors: Horowitz
authors: Yajue
authors: Y
authors: Huang
authors: Meora
authors: M
authors: Feinmesser
authors: J Steve
authors: JS
authors: Hou
authors: Brianna
authors: B
authors: St Cyr
authors: Ilanit
authors: I
authors: Burnstein
authors: Hadas
authors: H
authors: Gibori
authors: Nir
authors: N
authors: Dromi
authors: Mats
authors: M
authors: Sanden
authors: Michal
authors: M
authors: Kushnir
authors: Ranit
authors: R
authors: Aharonov
mesh_terms: D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000145:classification* / Q000175:diagnosis* / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D035683:MicroRNAs