title: A highly specialized flavin mononucleotide riboswitch responds differently to similar ligands and confers roseoflavin resistance to Streptomyces davawensis.
abstract: Streptomyces davawensis is the only organism known to synthesize the antibiotic roseoflavin, a riboflavin (vitamin B2) analog. Roseoflavin is converted to roseoflavin mononucleotide (RoFMN) and roseoflavin adenine dinucleotide in the cytoplasm of target cells. (Ribo-)Flavin mononucleotide (FMN) riboswitches are genetic elements, which in many bacteria control genes responsible for the biosynthesis and transport of riboflavin. Streptomyces davawensis is roseoflavin resistant, and the closely related bacterium Streptomyces coelicolor is roseoflavin sensitive. The two bacteria served as models to investigate roseoflavin resistance of S. davawensis and to analyze the mode of action of roseoflavin in S. coelicolor. Our experiments demonstrate that the ribB FMN riboswitch of S. davawensis (in contrast to the corresponding riboswitch of S. coelicolor) is able to discriminate between the two very similar flavins FMN and RoFMN and shows opposite responses to the latter ligands.
authors: Fakultät für Biotechnologie, Institut für Technische Mikrobiologie, Hochschule Mannheim, Paul-Wittsack-Str. 10, 68163 Mannheim, Germany.
authors: Danielle Biscaro
authors: DB
authors: Pedrolli
authors: Andreas
authors: A
authors: Matern
authors: Joy
authors: J
authors: Wang
authors: Miriam
authors: M
authors: Ester
authors: Kathrin
authors: K
authors: Siedler
authors: Ronald
authors: R
authors: Breaker
authors: Matthias
authors: M
authors: Mack
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D052157:Aptamers, Nucleotide / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D025141:Drug Resistance, Fungal; D005486:Flavin Mononucleotide / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000031:analogs & derivatives / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D008024:Ligands; D017354:Point Mutation; D014176:Protein Biosynthesis; D012256:Riboflavin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D012258:Riboflavin Synthase / Q000378:metabolism; D058928:Riboswitch; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D048372:Streptomyces coelicolor / Q000187:drug effects / Q000201:enzymology / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D008024:Ligands; D058928:Riboswitch; C576820:roseoflavin adenine dinucleotide; C576819:roseoflavin mononucleotide; D005182:Flavin-Adenine Dinucleotide; C009191:roseoflavin; D005486:Flavin Mononucleotide; D012258:Riboflavin Synthase; D012256:Riboflavin