title: Direct bisulfite sequencing for examination of DNA methylation with gene and nucleotide resolution from brain tissues.
abstract: DNA methylation is an epigenetic modification that is essential for the development and mature function of the central nervous system. Due to the relevance of this modification to the transcriptional control of gene expression, it is often necessary to examine changes in DNA methylation patterns with both gene and single-nucleotide resolution. Here, we describe an in-depth basic protocol for direct bisulfite sequencing of DNA isolated from brain tissue, which will permit direct assessment of methylation status at individual genes as well as individual cytosine molecules/nucleotides within a genomic region. This method yields analysis of DNA methylation patterns that is robust, accurate, and reproducible, thereby allowing insights into the role of alterations in DNA methylation in brain tissue.
authors: Department of Neurobiology and Evelyn F. McKnight Brain Institute, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors: R Ryley
authors: RR
authors: Parrish
authors: Jeremy J
authors: JJ
authors: Day
authors: Farah D
authors: FD
authors: Lubin
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics*; D044127:Epigenesis, Genetic / Q000235:genetics*; D006801:Humans; D009711:Nucleotides / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D013447:Sulfites / Q000737:chemistry*
chemical_list: D009711:Nucleotides; D013447:Sulfites; C042345:hydrogen sulfite