title: Polypodium leucotomos decreases UV-induced epidermal cell proliferation and enhances p53 expression and plasma antioxidant capacity in hairless mice.
abstract: A single dose of ultraviolet radiation (UVR) induces significant changes in blood and skin of hairless mice. Oral administration of a hydrophilic extract of the fern Polypodium leucotomos (PL, 300 mg/kg during 5 days before UVR and for two additional days after irradiation) modulates some of the effects of UVR. Most significantly, PL administration reduced the number of proliferating cells by 13%, increased the number of p53(+) cells by 63%, enhanced the antioxidant plasma capacity (ORAC) by 30% and reinforced the network of dermal elastic fibres. Western blot analysis of skin antioxidant-related enzymes failed to demonstrate significant changes caused by PL. Thus, the beneficial effect of PL likely owes to its antioxidant and anti-ROS properties rather than its modulation of the expression of endogenous antioxidant systems. These data provide mechanistic clues for its efficacy as a systemic photoprotective agent with antioxidant and anti-photo-ageing properties.
authors: Department of Pharmacology, School of Medicine, Universitat "Rovira i Virgili", Reus, Spain.
authors: Esperanza
authors: E
authors: Rodríguez-Yanes
authors: Ángeles
authors: Á
authors: Juarranz
authors: Jesús
authors: J
authors: Cuevas
authors: Salvador
authors: S
authors: Gonzalez
authors: Jordi
authors: J
authors: Mallol
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects*; D004307:Dose-Response Relationship, Radiation; D004547:Elastic Tissue / Q000378:metabolism; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D023421:Models, Animal; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D032494:Polypodium; D015595:Skin Aging / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D016159:Tumor Suppressor Protein p53; D013482:Superoxide Dismutase