title: TubStain: a universal peptide-tool to label microtubules.
abstract: Imaging of the microtubular network is an important strategy to define cell-cycle specific and pathological states of eukaryotic cells. Here, we describe TubStain, a novel recombinant polypeptide allowing direct, non-antibody dependent labeling of microtubules in fixed cells over a broad range of different species and tissues. TubStain has, due to its small size and susceptibility for biochemical and genetic manipulations, high potential as a microtubule marker in state-of-the-art microscopy.
authors: Institut für Anatomie, Abteilung für Anatomie & Molekulare Embryologie, Ruhr-Universität, Bochum, Germany.
authors: Carsten
authors: C
authors: Theiss
authors: Alexander
authors: A
authors: Neuhaus
authors: Wolfgang
authors: W
authors: Schliebs
authors: Ralf
authors: R
authors: Erdmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008870:Microtubules; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D015202:Protein Engineering; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism; D014404:Tubulin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; C455539:PEX14 protein, human; D010455:Peptides; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014404:Tubulin