title: Protein phosphatase Z modulates oxidative stress response in fungi.
abstract: The genome of the filamentous fungus Aspergillus nidulans harbors the gene ppzA that codes for the catalytic subunit of protein phosphatase Z (PPZ), and the closely related opportunistic pathogen Aspergillus fumigatus encompasses a highly similar PPZ gene (phzA). When PpzA and PhzA were expressed in Saccharomyces cerevisiae or Schizosaccharomyces pombe they partially complemented the deleted phosphatases in the ppz1 or the pzh1 mutants, and they also mimicked the effect of Ppz1 overexpression in slt2 MAP kinase deficient S. cerevisiae cells. Although ppzA acted as the functional equivalent of the known PPZ enzymes its disruption in A. nidulans did not result in the expected phenotypes since it failed to affect salt tolerance or cell wall integrity. However, the inactivation of ppzA resulted in increased sensitivity to oxidizing agents like tert-butylhydroperoxide, menadione, and diamide. To demonstrate the general validity of our observations we showed that the deletion of the orthologous PPZ genes in other model organisms, such as S. cerevisiae (PPZ1) or Candida albicans (CaPPZ1) also caused oxidative stress sensitivity. Thus, our work reveals a novel function of the PPZ enzyme in A. nidulans that is conserved in very distantly related fungi.
authors: Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary.
authors: Éva
authors: É
authors: Leiter
authors: Asier
authors: A
authors: González
authors: Éva
authors: É
authors: Erdei
authors: Carlos
authors: C
authors: Casado
authors: László
authors: L
authors: Kovács
authors: Csaba
authors: C
authors: Ádám
authors: Judit
authors: J
authors: Oláh
authors: Márton
authors: M
authors: Miskei
authors: Monika
authors: M
authors: Molnar
authors: Ilona
authors: I
authors: Farkas
authors: Zsuzsanna
authors: Z
authors: Hamari
authors: Joaquín
authors: J
authors: Ariño
authors: István
authors: I
authors: Pócsi
authors: Viktor
authors: V
authors: Dombrádi
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics; D020134:Catalytic Domain; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D005656:Fungal Proteins