title: Photocontrol of tyrosine phosphorylation in mammalian cells via genetic encoding of photocaged tyrosine.
abstract: We report the first site-specific genetic encoding of photocaged tyrosine into proteins in mammalian cells. By photocaging Tyr701 of STAT1 we demonstrate that it is possible to photocontrol tyrosine phosphorylation and signal transduction in mammalian cells.
authors: Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom.
authors: Eyal
authors: E
authors: Arbely
authors: Jessica
authors: J
authors: Torres-Kolbus
authors: Alexander
authors: A
authors: Deiters
authors: Jason W
authors: JW
authors: Chin
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008027:Light; D017020:Methanosarcina / Q000201:enzymology / Q000235:genetics; D009154:Mutation; D010766:Phosphorylation; D015202:Protein Engineering / Q000379:methods; D050794:STAT1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014443:Tyrosine / Q000235:genetics / Q000378:metabolism*
chemical_list: D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D014443:Tyrosine; D000604:Amino Acyl-tRNA Synthetases