title: Fast microscopical dissection of action scenes played by Escherichia coli RNA polymerase.
abstract: Using fast-scanning atomic force microscopy, we directly visualized the interaction of Escherichia coli RNA polymerase (RNAP) with DNA at the scan rate of 1-2 frames per second. The analyses showed that the RNAP can locate the promoter region not only by sliding but also by hopping and/or segmental transfer. Upon the addition of 0.05 mM NTPs to the stalled complex, the RNAP molecule pulled the template DNA uni-directionally at the rates of 15 nucleotides/s on average. The present method is potentially applicable to examine a variety of protein-nucleic acid interactions, especially those involved in the process of gene regulation.
authors: Laboratory of Plasma Membrane and Nuclear Signaling, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan. ysuzuki79@kuchem.kyoto-u.ac.jp
authors: Yuki
authors: Y
authors: Suzuki
authors: Minsang
authors: M
authors: Shin
authors: Aiko
authors: A
authors: Yoshida
authors: Shige H
authors: SH
authors: Yoshimura
authors: Kunio
authors: K
authors: Takeyasu
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force / Q000379:methods; D011401:Promoter Regions, Genetic
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D012321:DNA-Directed RNA Polymerases