title: Changes in angiotensin II type 1 receptor expression in the rat bladder by bladder outlet obstruction.
abstract: OBJECTIVE
  To demonstrate the change in the expression of angiotensin II type 1 receptor (AT1) in the rat bladder with partial bladder outlet obstruction (P-BOO).
  
  
  METHODS
  Bladder specimens were obtained from 12-week-old Wistar female rats that were divided into two groups, a P-BOO group and a control group. The rats of the P-BOO group were divided into six groups: a sham-operated control group, 1 day postoperatively, 2 days postoperatively, 4 days postoperatively, 7 days postoperatively and 14 days postoperatively. The cystometric findings and immunohistochemical staining of the detrusor muscle with the AT1 antibody were compared in each group.
  
  
  RESULTS
  AT1 localized on the cell membrane of the detrusor smooth muscle and in cytoplasm of suburothelial myofibroblasts in the control rats. The expression of AT1 disappeared in the detrusor muscle and suburothelial myofibroblasts in P-BOO, but AT1 was highly expressed in urothelial cells 1 day after surgery. The expression of AT1 in urothelial cells gradually decreased with time after surgery. AT1 completely disappeared in urothelial cells 14 days after surgery.
  
  
  CONCLUSIONS
  The present study demonstrated that the site of AT1 expression changes in response to the mechanical stress caused by P-BOO, and finally there was no expression of AT1 in rat bladder tissue following P-BOO. These data suggest the change in AT1 expression may play a role in bladder function.
authors: Division of Nephro-Urology, Unit of Translational Medicine, Department of Medical and Dental Sciences, Nagasaki, Japan. toubu7 @ hotmail.com
authors: Shohei
authors: S
authors: Tobu
authors: Mitsuru
authors: M
authors: Noguchi
authors: Teppei
authors: T
authors: Hatada
authors: Ken-ichi
authors: K
authors: Mori
authors: Manabu
authors: M
authors: Matsuo
authors: Hideki
authors: H
authors: Sakai
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000379:methods; D009130:Muscle, Smooth / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D044140:Receptor, Angiotensin, Type 1 / Q000096:biosynthesis*; D013314:Stress, Mechanical; D013997:Time Factors; D001743:Urinary Bladder / Q000378:metabolism* / Q000473:pathology; D001748:Urinary Bladder Neck Obstruction / Q000378:metabolism / Q000503:physiopathology*; D019459:Urothelium / Q000473:pathology
chemical_list: D044140:Receptor, Angiotensin, Type 1