title: A palmtop-sized microfluidic cell culture system driven by a miniaturized infusion pump.
abstract: A palmtop-sized microfluidic cell culture system is presented. The system consists of a microfluidic device and a miniaturized infusion pump that possesses a reservoir of culture medium, an electrical control circuit, and an internal battery. The footprint of the system was downsized to 87 × 57 mm, which is, to the best of our knowledge, the smallest integrated cell culture system. Immortalized human microvascular endothelial cells (HMEC-1) and human umbilical vein endothelial cells (HUVEC) were cultured in the system. HMEC-1 in the system proliferated at the same speed as cells in a microchannel perfused by a syringe pump and cells in a culture flask. HUVEC in the system oriented along the direction of the fluid flow. Claudin-5, a tight junction protein, was localized along the peripheries of the HUVEC. We expect that the present system is applicable to various cell types as a stand-alone and easy-to-use system for microfluidic bioanalysis.
authors: Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Mejirodai, Bunkyo-ku, Tokyo, Japan.
authors: Naoki
authors: N
authors: Sasaki
authors: Mika
authors: M
authors: Shinjo
authors: Satoshi
authors: S
authors: Hirakawa
authors: Masahiro
authors: M
authors: Nishinaka
authors: Yo
authors: Y
authors: Tanaka
authors: Kazuma
authors: K
authors: Mawatari
authors: Takehiko
authors: T
authors: Kitamori
authors: Kae
authors: K
authors: Sato
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D049109:Cell Proliferation; D062507:Claudin-5; D057167:Claudins / Q000737:chemistry / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008904:Miniaturization / Q000295:instrumentation*; D019108:Tight Junctions / Q000737:chemistry / Q000378:metabolism
chemical_list: C107466:CLDN5 protein, human; D062507:Claudin-5; D057167:Claudins