title: Targeted therapy for glioma using cyclic RGD-entrapped polyionic complex nanomicelles.
abstract: BACKGROUND
  The purpose of this study was to test the efficacy of cyclic Arg-Gly-Asp (RGD) peptide conjugated with polyionic complex nanomicelles as targeted therapy for glioma.
  
  
  METHODS
  A stable cyclic RGD polyionic complex nanostructure, ie, a c(RGDfC) polyionic complex micelle, was synthesized and its biocompatibility with cultured neurons was assessed using a cell viability assay. Targeted binding to cultured glioma cells was evaluated by the CdTe quantum dot marking technique and a cell viability assay. The inhibitory effect of the nanomicelles against glioma cells was also evaluated, and their targeted migration into rat brain glioma cells and apoptotic effects were traced by the CdTe quantum dot marking and immunohistochemical staining.
  
  
  RESULTS
  c(RGDfC) polyionic complex micelles did not affect the growth of neurons but bonded selectively to and inhibited proliferation of glioma cells in vitro. When tested in vivo, the micelles migrated into glioma cells, inducing apoptosis in the rat brain.
  
  
  CONCLUSIONS
  The c(RGDfC) polyionic complex micelle is an effective targeted therapy against glioma.
authors: Department of Neurology, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai China. yaorong_liu8@hotmail.com
authors: Xiaoying
authors: X
authors: Liu
authors: Wenguo
authors: W
authors: Cui
authors: Bo
authors: B
authors: Li
authors: Zhen
authors: Z
authors: Hong
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D017209:Apoptosis / Q000187:drug effects; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D019187:Cadmium Compounds / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems / Q000379:methods*; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D008422:Materials Testing; D008823:Micelles; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D009842:Oligopeptides / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D045663:Quantum Dots; D051381:Rats; D017208:Rats, Wistar; D013691:Tellurium / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D019187:Cadmium Compounds; D008823:Micelles; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D013691:Tellurium; C028337:cadmium telluride