title: Nanoglue: an alternative way to display cell-internalizing peptide at the spikes of hepatitis B virus core nanoparticles for cell-targeting delivery.
abstract: Cell-internalizing peptides (CIPs) can be used to mediate specific delivery of nanoparticles across cellular membrane. The objective of this study was to develop a display technique using hepatitis B virus (HBV) capsid-binding peptide as a "nanoglue" to present CIPs on HBV nanoparticles for cell-targeting delivery. A CIP was selected from a phage display library and cross-linked specifically at the tips of the spikes of the HBV capsid nanoparticle via the "nanoglue" by using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS). Fluorescent oligonucleotides packaged in the nanoparticles and the fluorescein molecules conjugated on the nanoparticles were delivered to cells by using this display technique. This study demonstrated a proof of principle for cell-targeting delivery via "nanoglue" bioconjugation.
authors: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors: Khai Wooi
authors: KW
authors: Lee
authors: Beng Ti
authors: BT
authors: Tey
authors: Kok Lian
authors: KL
authors: Ho
authors: Bimo A
authors: BA
authors: Tejo
authors: Wen Siang
authors: WS
authors: Tan
mesh_terms: D002213:Capsid / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry / Q000493:pharmacokinetics*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D006367:HeLa Cells; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000378:metabolism*; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D009841:Oligonucleotides / Q000493:pharmacokinetics; D010455:Peptides / Q000737:chemistry / Q000493:pharmacokinetics*; D013388:Succinimides / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D036022:Capsid Proteins; C465543:N-hydroxysulfosuccimide; D009841:Oligonucleotides; D010455:Peptides; D013388:Succinimides; D014764:Viral Proteins; D005022:Ethyldimethylaminopropyl Carbodiimide