title: Use of biotinylated chitosan for substrate-mediated gene delivery.
abstract: To improve transfection efficiency of nonviral vectors, biotinylated chitosan was applied to complex with DNA in different N/P ratios. The morphologies and the sizes of formed nanoparticles were suitable for cell uptake. The biotinylation decreased the surface charges of nanoparticles and hence reduced the cytotoxicity. The loading capacities of chitosan were slightly decreased with the increase of biotinylation, but most of the DNA molecules were still complexed. Using different avidin-coated surfaces, the interaction between biotinylated nanoparticles to the substrate may be manipulated. The in vitro transfection results demonstrated that biotinylated nanoparticles may be bound to avidin coated surfaces, and the transfection efficiencies were thus increased. Through regulating the N/P ratio, biotinylation levels, and surface avidin, the gene delivery can be optimized. Compared to the nonmodified chitosan, biotinylated nanoparticles on biomaterial surfaces can increase their chances to contact adhered cells. This spatially controlled gene delivery improved the gene transfer efficiency of nonviral vectors and could be broadly applied to different biomaterial scaffolds for tissue engineering applications.
authors: Department of Chemical and Materials Engineering, National Central University, Jhongli City, Taiwan. huweiwen@cc.ncu.edu.tw
authors: Wei-Wen
authors: WW
authors: Hu
authors: Wun-Jheng
authors: WJ
authors: Syu
authors: Wen-Yih
authors: WY
authors: Chen
authors: Ruoh-Chyu
authors: RC
authors: Ruaan
authors: Yu-Che
authors: YC
authors: Cheng
authors: Chih-Cheng
authors: CC
authors: Chien
authors: Chuan
authors: C
authors: Li
authors: Chih-Ang
authors: CA
authors: Chung
authors: Chia-Wen
authors: CW
authors: Tsao
mesh_terms: D001360:Avidin / Q000378:metabolism; D001692:Biological Transport; D001710:Biotin / Q000378:metabolism; D019921:Biotinylation; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D057809:HEK293 Cells; D006801:Humans; D008024:Ligands; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry; D013499:Surface Properties; D014162:Transfection / Q000379:methods*
chemical_list: D004337:Drug Carriers; D008024:Ligands; D001360:Avidin; D001710:Biotin; D004247:DNA; D048271:Chitosan