title: Phaeocystidibacter luteus gen. nov., sp. nov., a member of the family Cryomorphaceae isolated from the marine alga Phaeocystis globosa, and emended description of Owenweeksia hongkongensis.
abstract: A taxonomic study was carried out on strain PG2S01(T), isolated from a culture of Phaeocystis globosa, a haemolytic, toxin-producing, harmful marine alga. Cells of strain PG2S01(T) were Gram-negative, strictly aerobic, non-motile, non-fermentative, orange-pigmented, moderately halophilic rods. Growth was observed in the presence of 0.25-7.5 % NaCl and at 10-40 °C. The dominant fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 0 3-OH, iso-C16 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH. The major polar lipids were phosphatidylethanolamine and several unidentified aminolipids, phospholipids and other lipids. The G+C content of the chromosomal DNA was 44.6 mol%. 16S rRNA gene sequence comparison showed that strain PG2S01(T) was most closely related to Owenweeksia hongkongensis UST20020801(T) (88.9 % 16S rRNA gene sequence similarity); levels of similarity between strain PG2S01(T) and the type strains of recognized representatives of genera in the family Cryomorphaceae were <88 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PG2S01(T) formed a distinct evolutionary lineage within the family Cryomorphaceae. Strain PG2S01(T) was distinguishable from members of phylogenetically related genera by differences in several phenotypic properties. On the basis of phenotypic data and phylogenetic inference, strain PG2S01(T) represents a novel species in a new genus in the family Cryomorphaceae, for which the name Phaeocystidibacter luteus gen. nov., sp. nov. is proposed. The type strain of the type species is PG2S01(T) ( = CCTCC AB 209288(T)  = LMG 25704(T)  = MCCC 1F01079(T)). An emended description of O. hongkongensis Lau et al. 2005 is also proposed.
authors: State Key Laboratory of Marine Environmental Science and Key Laboratory of the MOE for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors: Yanyan
authors: Y
authors: Zhou
authors: The Institute of Urban Environment (IUE), Chinese Academy of Sciences, Xiamen 361005, PR China.|State Key Laboratory of Marine Environmental Science and Key Laboratory of the MOE for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors: Jianqiang
authors: J
authors: Su
authors: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.|State Key Laboratory of Marine Environmental Science and Key Laboratory of the MOE for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors: Qiliang
authors: Q
authors: Lai
authors: State Key Laboratory of Marine Environmental Science and Key Laboratory of the MOE for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors: Xinyi
authors: X
authors: Li
authors: The Institute of Urban Environment (IUE), Chinese Academy of Sciences, Xiamen 361005, PR China.|State Key Laboratory of Marine Environmental Science and Key Laboratory of the MOE for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors: Xiaoru
authors: X
authors: Yang
authors: State Key Laboratory of Marine Environmental Science and Key Laboratory of the MOE for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors: Peiyan
authors: P
authors: Dong
authors: State Key Laboratory of Marine Environmental Science and Key Laboratory of the MOE for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
authors: Tianling
authors: T
authors: Zheng
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D058087:Haptophyta / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6