title: Brain microvascular endothelial cell transplantation ameliorates ischemic white matter damage.
abstract: Ischemic insults affecting the internal capsule result in sensory-motor disabilities which adversely affect the patient's life. Cerebral endothelial cells have been reported to exert a protective effect against brain damage, so the transplantation of healthy endothelial cells might have a beneficial effect on the outcome of ischemic brain damage. In this study, endothelin-1 (ET-1) was injected into the rat internal capsule to induce lacunar infarction. Seven days after ET-1 injection, microvascular endothelial cells (MVECs) were transplanted into the internal capsule. Meningeal cells or 0.2% bovine serum albumin-Hank's balanced salt solution were injected as controls. Two weeks later, the footprint test and histochemical analysis were performed. We found that MVEC transplantation improved the behavioral outcome based on recovery of hind-limb rotation angle (P<0.01) and induced remyelination (P<0.01) compared with the control groups. Also the inflammatory response was repressed by MVEC transplantation, judging from fewer ED-1-positive activated microglial cells in the MVEC-transplanted group than in the other groups. Elucidation of the mechanisms by which MVECs ameliorate ischemic damage of the white matter may provide important information for the development of effective therapies for white matter ischemia.
authors: Department of Neurosurgery, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
authors: Sandra
authors: S
authors: Puentes
authors: Masashi
authors: M
authors: Kurachi
authors: Koji
authors: K
authors: Shibasaki
authors: Masae
authors: M
authors: Naruse
authors: Yuhei
authors: Y
authors: Yoshimoto
authors: Masahiko
authors: M
authors: Mikuni
authors: Hideaki
authors: H
authors: Imai
authors: Yasuki
authors: Y
authors: Ishizaki
mesh_terms: D000818:Animals; D001921:Brain / Q000503:physiopathology*; D002545:Brain Ischemia / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D042783:Endothelial Cells / Q000637:transplantation*; D019332:Endothelin-1; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009413:Nerve Fibers, Myelinated / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology
chemical_list: D019332:Endothelin-1