title: A study on the estrogen receptor α gene polymorphism and its expression in thin endometrium of unknown etiology.
abstract: OBJECTIVE
  To study the relationship between the estrogen receptor α (ERa) gene polymorphism and expression in thin endometrium.
  
  
  METHODS
  We chose 120 unknown thin endometrium patients as the case group and another 112 normal endometrium women as the control group. Methods in molecular biology were used to analyze restriction fragment length polymorphism of PvuII and XbaI in the ERa gene. RT-PCR and Western blot were used to investigate the expressions of the ERa at mRNA and protein levels.
  
  
  RESULTS
  P genotypic frequency was 47.1% in the case group and 30.0% in the control group; the OR was 2.076. X genotypic frequency in the case group was 20.8%, and 30.4% in the control group; the OR was 0.602. Restriction fragment length polymorphism of PvuII and XbaI in the two groups were distributed with polymorphisms. The mRNA and protein expressions of ERa decreased compared with that in the control group (p < 0.05).
  
  
  CONCLUSIONS
  ERa gene polymorphism is related to unknown thin endometrium, in which P allele may be the risk and X allele may be its guard factor. The expression of ERa decreases in thin endometrium compared with normal endometrium, and ERa may be related to the unknown etiological thin endometrium.
authors: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. yrui96@126.com
authors: Rui
authors: R
authors: Yuan
authors: Ai-Wen
authors: AW
authors: Le
mesh_terms: D000328:Adult; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology*; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D006801:Humans; D009929:Organ Size; D011110:Polymorphism, Genetic; D014591:Uterine Diseases / Q000209:etiology / Q000473:pathology*; D055815:Young Adult
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha