title: Reduced expression of psoriasin in human airway cystic fibrosis epithelia.
abstract: Psoriasin is a low molecular weight Ca(2+)-binding protein with known antimicrobial activity. Since human airway epithelial cells produce a number of powerful antimicrobial agents as part of their host defence, we investigated whether psoriasin was expressed in human bronchial epithelial cell lines. Expression was investigated in 16HBE14o- cells, derived from a normal individual, and compared to CFBE41o- cells, derived from a cystic fibrosis patient. We also examined psoriasin expression following treatment with factors pertinent to the CF lung-oxidant stress and exposure to pro-inflammatory cytokines. CFBE41o- cells demonstrated much reduced psoriasin levels compared to the 16HBE14o- cells. Increased psoriasin expression was seen following treatment with IL-22 and a cytomix of the pro-inflammatory cytokines IL-1β, TNF-α and IFN-γ; however, the oxidant stressor tert-butyl hydroperoxide had no apparent effect. Over-expression of human psoriasin into both cell lines resulted in increased internalization of Pseudomonas aeruginosa. In conclusion, expression of psoriasin - which has known anti-microbial activity in other systems - appears to be reduced in CFBE410- compared to 16HBE14o- cells, and its expression modified by exposure to pro-inflammatory cytokines.
authors: Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia, Canada. TS514830@dal.ca
authors: Tiesong
authors: T
authors: Li
authors: Elizabeth A
authors: EA
authors: Cowley
mesh_terms: D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000378:metabolism* / Q000473:pathology; D016207:Cytokines / Q000502:physiology; D005786:Gene Expression Regulation; D006801:Humans; D018836:Inflammation Mediators / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000473:pathology; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; C089242:S100A7 protein, human