title: Associations between polymorphisms in IL-12A, IL-12B, IL-12Rβ1, IL-27 gene and serum levels of IL-12p40, IL-27p28 with esophageal cancer.
abstract: OBJECTIVE
  The aim of this study was to investigate whether IL-12A, IL-12B, IL-12Rβ1, and IL-27 gene polymorphisms and serum levels of IL-12, IL-27 are associated with esophageal cancer.
  
  
  METHODS
  We genotyped IL-12A gene rs568408, IL-12B gene rs3212227, IL-12Rβ1 gene 378 C/G, IL-27 gene rs153109, rs17855750, and rs181206 polymorphisms in a case-control study of 426 esophageal cancer patients and 432 health controls, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and serum IL-12p40 and IL-27p28 levels were measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Both serum IL-12p40 and IL-27p28 levels were significantly higher in controls than those in patients (P < 0.01). Rs568408 AG/AA, rs3212227 CC/AC, and IL-12Rβ1 378 GG/GC genotypes were associated with significantly increased risk of esophageal cancer (rs568408: χ(2) = 5.704, P = 0.017; rs3212227: χ(2) = 7.689, P = 0.006; IL-12Rβ1 378C/G: χ(2) = 5.206, P = 0.023). Moreover, rs3212227 CC/AC and 378 GG/GC genotypes were observed significantly associated with decreased serum IL-12p40 level in patients compare to other genotypes (rs3212227: t = 2.129, P = 0.034; IL-12Rβ1 378 C/G: t = 2.178, P = 0.030). Furthermore, frequency of rs3212227 CC/AC genotypes was significantly higher in patients with poor differentiation than those with AA genotype (χ(2) = 4.314, P = 0.035).
  
  
  CONCLUSIONS
  Our data suggest that the impaired production of IL-12p40 and IL-27p28 behaves as risk factors for esophageal cancer occurrence. IL-12B gene rs3212227 CC/AC and IL-12Rβ1 gene 378 GG/GC genotypes, which associated with decreased IL-12p40 level, may contribute to esophageal cancer susceptibility.
authors: Department of Thoracic Surgery, First Affiliated Hospital of Xinxiang Medical University, Weihui City, 453100 Henan Province, People's Republic of China.
authors: Yi-Peng
authors: YP
authors: Tao
authors: Wan-Ling
authors: WL
authors: Wang
authors: Song-Yue
authors: SY
authors: Li
authors: Jian
authors: J
authors: Zhang
authors: Qi-Zhong
authors: QZ
authors: Shi
authors: Fen
authors: F
authors: Zhao
authors: Bao-Sheng
authors: BS
authors: Zhao
mesh_terms: D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D004938:Esophageal Neoplasms / Q000097:blood / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics*; D053762:Interleukin-12 Subunit p40 / Q000097:blood* / Q000235:genetics; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D021122:Protein Subunits / Q000097:blood; D053707:Receptors, Interleukin-12 / Q000235:genetics*
chemical_list: D053762:Interleukin-12 Subunit p40; D007378:Interleukins; C464565:MYDGF protein, human; D021122:Protein Subunits; D053707:Receptors, Interleukin-12; D018664:Interleukin-12