title: Micro-autoradiographic assessment of cell types contributing to 2-deoxy-2-[(18)F]fluoro-D-glucose uptake during ventilator-induced and endotoxemic lung injury.
abstract: OBJECTIVE
  The aim of the study was to use micro-autoradiography to investigate the lung cell types responsible for 2-deoxy-2-[(18)F]fluoro-D-glucose (FDG) uptake in murine models of acute lung injury (ALI).
  
  
  METHODS
  C57/BL6 mice were studied in three groups: controls, ventilator-induced lung injury (VILI), and endotoxin. VILI was produced by high tidal volumes and zero end-expiratory pressure and endotoxin ALI, by intranasal administration. Following FDG injection, the lungs were processed and exposed to autoradiographic emulsion. Grain density over cells was used to quantify FDG uptake.
  
  
  RESULTS
  Neutrophils, macrophages, and type 2 epithelial cells presented higher grain densities during VILI and endotoxin ALI than controls. Remarkably, cell grain density in specific cell types was dependent on the injury mechanism. Whereas macrophages showed high grain densities during endotoxin ALI, similar to those exhibited by neutrophils, type 2 epithelial cells demonstrated the second highest grain density (with neutrophils as the highest) during VILI.
  
  
  CONCLUSIONS
  In murine models of VILI and endotoxin ALI, FDG uptake occurs not only in neutrophils but also in macrophages and type 2 epithelial cells. FDG uptake by individual cell types depends on the mechanism underlying ALI.
authors: Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, 55 Fruit St., Boston, MA 02114, USA.
authors: Dalia
authors: D
authors: Saha
authors: Kazue
authors: K
authors: Takahashi
authors: Nicolas
authors: N
authors: de Prost
authors: Tilo
authors: T
authors: Winkler
authors: Miguel
authors: M
authors: Pinilla-Vera
authors: Rebecca M
authors: RM
authors: Baron
authors: Marcos F
authors: MF
authors: Vidal Melo
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism / Q000473:pathology; D000704:Analysis of Variance; D000818:Animals; D001345:Autoradiography / Q000379:methods*; D042783:Endothelial Cells / Q000737:chemistry / Q000378:metabolism*; D019446:Endotoxemia / Q000378:metabolism* / Q000473:pathology; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000737:chemistry / Q000493:pharmacokinetics*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008853:Microscopy; D057054:Molecular Imaging / Q000379:methods; D009504:Neutrophils / Q000737:chemistry / Q000378:metabolism*; D019275:Radiopharmaceuticals / Q000737:chemistry / Q000493:pharmacokinetics*; D055397:Ventilator-Induced Lung Injury / Q000378:metabolism* / Q000473:pathology
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18