title: Fabrication of aligned poly(lactic acid)-chitosan nanofibers by novel parallel blade collector method for skin tissue engineering.
abstract: Poly(lactic acid) (PLA) was blended with chitosan (CS) to fabricate electrospun aligned PLA-CS nanofibers. These prepared nanofibers were aligned using a novel collector made of parallel blades which is designed to increase the transversal electric field across the gap. SEM images show that the fiber diameter mostly ranges between 150 +/- 60 nm and Fourier Transform infrared Spectroscopy (FTIR) analysis confirm the presence of PLA and CS. X-Ray Diffraction (XRD) studies explains the amorphous nature of electrospun PLA-CS nanofibers, suitable for faster degradation. Degradation studies confirmed that PLA-CS nanofiber has enhanced degradation than the pure PLA fibers. Cell studies with human dermal fibroblasts (HDF) show the orientation of cells along the direction of fiber alignment. The results indicate that the prepared PLA-CS aligned nanofibers are promising material for skin tissue engineering.
authors: Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
authors: K T
authors: KT
authors: Shalumon
authors: D
authors: D
authors: Sathish
authors: S V
authors: SV
authors: Nair
authors: K P
authors: KP
authors: Chennazhi
authors: H
authors: H
authors: Tamura
authors: R
authors: R
authors: Jayakumar
mesh_terms: D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation; D010316:Particle Size; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D012867:Skin / Q000166:cytology / Q000254:growth & development*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan