title: Increased expression of vascular endothelial growth factor in neo-vascularized canine brain tissue.
abstract: This retrospective study was done to characterize the levels of vascular endothelial growth factor (VEGF) and hypoxia inducible factor 1 (HIF-1α) in dog brains with neo-vascularization in the cerebral cortex of frontal, temporal, and parietal lobe by using immunohistochemistry (IHC) and Western blot. In neo-vascularized (NV) brains, we analyzed the number and area of blood vessels and the expression of VEGF and HIF-1α. The IHC results showed that the number and area of blood vessels, as assessed by immunolabeling for von Willebrand factor, was higher in the NV brain than in the control brain. The Western blot results showed that the level of VEGF was increased, predominantly in NV brain of the cerebral cortex relative to the clinically normal cerebral cortex, whereas the expression of HIF-1α in NV brains was not different from the control brains. Our study showed that dilatation of vessels and development of new vessels in the cerebral cortex were observed in cases of canine CNS disease and found increased expression of VEGF in canine brains with neo-vascularization.
authors: Department of Veterinary Pathology, Small Animal Tumor Diagnostic Center, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong Gwangjin-gu, Seoul, 143-701 Republic of Korea.
authors: Chi-Ho
authors: CH
authors: Yu
authors: Ji-Young
authors: JY
authors: Yhee
authors: Jong-Hyuk
authors: JH
authors: Kim
authors: Keum-Soon
authors: KS
authors: Im
authors: Na-Hyun
authors: NH
authors: Kim
authors: So-Young
authors: SY
authors: Kwon
authors: Tai-Young
authors: TY
authors: Hur
authors: Jung-Hyang
authors: JH
authors: Sur
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D001921:Brain / Q000098:blood supply* / Q000378:metabolism*; D002493:Central Nervous System Diseases / Q000378:metabolism / Q000662:veterinary*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D009389:Neovascularization, Pathologic / Q000378:metabolism; D012189:Retrospective Studies; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A