title: Chitosan/bioactive glass nanoparticle composite membranes for periodontal regeneration.
abstract: Barrier membranes are used in periodontal applications with the aim of supporting periodontal regeneration by physically blocking migration of epithelial cells. The present work proposes a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce a novel guided tissue and bone regeneration membrane, fabricated by solvent casting. The CHT/BG-NP nanocomposite membranes are characterized in terms of water uptake, in mechanical tests, under simulated physiological conditions and in in vitro bioactivity tests. The addition of BG-NPs to CHT membranes decreased the mechanical potential of these membranes, but on the other hand the bioactivity improved. The membranes containing the BG-NPs induced the precipitation of bone-like apatite in simulated body fluid (SBF). Biological tests were carried out using human periodontal ligament cells and human bone marrow stromal cells. CHT/BG-NP composite membranes promoted cell metabolic activity and mineralization. The results indicate that the CHT/BG-NP composite membrane could potentially be used as a temporary guided tissue regeneration membrane in periodontal regeneration, with the possibility to induce bone regeneration.
authors: 3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal.
authors: Joana
authors: J
authors: Mota
authors: Na
authors: N
authors: Yu
authors: Sofia G
authors: SG
authors: Caridade
authors: Gisela M
authors: GM
authors: Luz
authors: Manuela E
authors: ME
authors: Gomes
authors: Rui L
authors: RL
authors: Reis
authors: John A
authors: JA
authors: Jansen
authors: X Frank
authors: XF
authors: Walboomers
authors: João F
authors: JF
authors: Mano
mesh_terms: D002118:Calcium / Q000378:metabolism; D002516:Ceramics / Q000737:chemistry*; D048271:Chitosan / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D005453:Fluorescence; D005898:Glass / Q000737:chemistry*; D016557:Guided Tissue Regeneration, Periodontal; D006801:Humans; D008567:Membranes, Artificial; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010078:Oxazines / Q000378:metabolism; D010513:Periodontal Ligament / Q000187:drug effects / Q000502:physiology; D013052:Spectrometry, X-Ray Emission; D013194:Staining and Labeling; D013314:Stress, Mechanical; D014867:Water / Q000737:chemistry; D014966:Xanthenes / Q000378:metabolism
chemical_list: D008567:Membranes, Artificial; D010078:Oxazines; D014966:Xanthenes; C416205:calcium oxide-silicon dioxide-diphosphorus pentoxide glass; D014867:Water; C005843:resazurin; D004247:DNA; D048271:Chitosan; D002118:Calcium