title: Physicochemical characterization and biocompatibility in vitro of biphasic calcium phosphate/polyvinyl alcohol scaffolds prepared by freeze-drying method for bone tissue engineering applications.
abstract: In this study, a well developed porous biphasic calcium phosphate (BCP)/polyvinyl alcohol (PVA) scaffold was prepared by emulsion foam freeze-drying method possessed moderate inter-connected pores and porosity. The SEM analysis showed that BCP nano-particles could disperse uniformly in the scaffolds, and the pore size, porosity, and compressive strength could be controlled by the weight ratio of BCP/PVA. The in vitro degradation and cytocompatibility of scaffolds were examined in this study. The degradation analysis showed the prepared scaffolds have a low variation of pH values (approximately 7.18-7.36) in SBF solution, and have the biodegradation rate of BCP/PVA scaffolds decreased with the increase of PVA concentration. Moreover, MTT assay indicated that the BCP/PVA porous scaffold has no negative effects on cells growth and proliferation, and the hBMSCs possessed a favorable spreading morphology on the BCP/PVA scaffold surface. The inter-connected pore structure, mechanical strength, biodegradation rate and cytocompatibility of the prepared BCP/PVA scaffold can meet essential requirements for blame bearing bone tissue engineering and regeneration.
authors: State Key Laboratory of Mould Technology, College of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan, PR China.
authors: Lei
authors: L
authors: Nie
authors: Dong
authors: D
authors: Chen
authors: Jinping
authors: J
authors: Suo
authors: Peng
authors: P
authors: Zou
authors: Shuibin
authors: S
authors: Feng
authors: Qi
authors: Q
authors: Yang
authors: Shuhua
authors: S
authors: Yang
authors: Shunan
authors: S
authors: Ye
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019245:Compressive Strength; D005612:Freeze Drying; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate; D011142:Polyvinyl Alcohol