title: IL-13-mediated immunological control of enterochromaffin cell hyperplasia and serotonin production in the gut.
abstract: Enterochromaffin (EC) cells in the gastrointestinal (GI) mucosa are the main source of serotonin (5-hydroxytryptamine (5-HT)) in the body. 5-HT is implicated in the pathophysiology of many GI disorders including functional and inflammatory bowel disorders. Herein we studied the role of interleukin 13 (IL-13) in EC cell biology by utilizing IL-13-deficient (IL-13-/-) mice and BON cells (a model for human EC cells). The numbers of EC cells and 5-HT amount were significantly lower in enteric parasite, Trichuris muris-infected IL-13-/- mice compared with the wild-type mice. This was accompanied with increased parasite burden in IL-13-/- mice. Treatment of naive and infected IL-13-/- mice with IL-13 increased EC cell numbers and 5-HT amount. BON cells expressed IL-13 receptor and in response to IL-13 produced more 5-HT. These results provide novel information on IL-13-mediated immunological control of 5-HT in the gut, which may ultimately lead to improved therapeutic opportunities in various GI disorders.
authors: Farncombe Family Digestive Health Research Institute, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.
authors: M
authors: M
authors: Manocha
authors: M S
authors: MS
authors: Shajib
authors: M M
authors: MM
authors: Rahman
authors: H
authors: H
authors: Wang
authors: P
authors: P
authors: Rengasamy
authors: M
authors: M
authors: Bogunovic
authors: M
authors: M
authors: Jordana
authors: L
authors: L
authors: Mayer
authors: W I
authors: WI
authors: Khan
mesh_terms: D000818:Animals; D002460:Cell Line; D004759:Enterochromaffin Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D041981:Gastrointestinal Tract / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D006965:Hyperplasia; D018793:Interleukin-13 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009092:Mucous Membrane / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D012701:Serotonin / Q000096:biosynthesis; D014257:Trichuriasis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D014258:Trichuris / Q000276:immunology; D014365:Tryptophan Hydroxylase / Q000235:genetics / Q000378:metabolism
chemical_list: D018793:Interleukin-13; D012701:Serotonin; D014365:Tryptophan Hydroxylase