title: Influence of pollution history on the response of coastal bacterial and nanoeukaryote communities to crude oil and biostimulation assays.
abstract: Pollution history has often been proposed to explain site-dependent bioremediation efficiencies, but this hypothesis has been poorly explored. Here, bacteria and their heterotrophic nanoflagellates (HNF) predators originating from pristine and chronically oil-polluted coastal sites were subjected to crude oil ± nutrients or emulsifier amendments. The addition of crude oil had a more visible effect on bacteria originating from the pristine site with a higher increase in the activity of given OTU and inactivation of other petroleum-sensitive bacteria, as revealed by DNA and RNA-based comparison. Such changes resulted in a delay in microbial growth and in a lower bacterial degradation of the more complex hydrocarbons. Biostimulation provoked a selection of different bacterial community assemblages and stirred metabolically active bacteria. This resulted in a clear increase of the peak of bacteria and their HNF predators and higher oil degradation, irrespective of the pollution history of the site.
authors: CNRS, UMR 7621, Laboratoire d'Océanographie Microbienne LOMIC, Avenue Fontaulé, F-66651 Banyuls-sur-Mer, France.
authors: Caroline
authors: C
authors: Sauret
authors: Urania
authors: U
authors: Christaki
authors: Paraskevi
authors: P
authors: Moutsaki
authors: Ioannis
authors: I
authors: Hatzianestis
authors: Alexandra
authors: A
authors: Gogou
authors: Jean-François
authors: JF
authors: Ghiglione
mesh_terms: D001419:Bacteria / Q000145:classification / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D017753:Ecosystem; D045703:Emulsifying Agents / Q000494:pharmacology; D056890:Eukaryota / Q000145:classification / Q000187:drug effects* / Q000378:metabolism; D005502:Food; D006838:Hydrocarbons / Q000032:analysis; D010578:Petroleum / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000494:pharmacology*; D014876:Water Pollution
chemical_list: D045703:Emulsifying Agents; D006838:Hydrocarbons; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D014874:Water Pollutants, Chemical