title: α-Synuclein modulates neurite outgrowth by interacting with SPTBN1.
abstract: α-Synuclein is the major component of Lewy bodies and Lewy neurites, the pathological hallmarks of surviving neuronal cells in Parkinson's disease patients. However, the physiological role played by α-synuclein remains unclear. In this study, spectrin beta non-erythrocyte 1 (SPTBN1) interacted with α-synuclein in phage display assays using a normalized human brain cDNA library. A direct interaction between α-synuclein and SPTBN1 was confirmed by GST pull-down and co-immunoprecipitation assays. SPTBN1 and α-synuclein proteins colocalized in N2a neuronal cells. Transfection of SPTBN1 caused human SH-SY5Y dopaminergic neuron cells to inappropriately induce neurites, which extended from cell bodies. Cotransfection with α-synuclein reversed SPTBN1-induced excessive neurite branching in SH-SY5Y cells, and only a single neurite extended from each neuron. These results suggest that α-synuclein modulates neurite outgrowth by interacting with cytoskeletal proteins such as SPTBN1.
authors: Department of Molecular Biology, Sejong University, 98 Gunja-dong, Kwangjin-gu, Seoul 143-747, Republic of Korea.
authors: Hak Joo
authors: HJ
authors: Lee
authors: Kyunghee
authors: K
authors: Lee
authors: Hana
authors: H
authors: Im
mesh_terms: D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D059290:Dopaminergic Neurons / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D016501:Neurites / Q000502:physiology*; D010300:Parkinson Disease / Q000378:metabolism; D013049:Spectrin / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D051844:alpha-Synuclein / Q000378:metabolism*
chemical_list: C452439:SPTBN1 protein, human; D051844:alpha-Synuclein; D013049:Spectrin