title: miR395 is a general component of the sulfate assimilation regulatory network in Arabidopsis.
abstract: In plants, microRNAs play an important role in many regulatory circuits, including responses to environmental cues such as nutrient limitations. One such microRNA is miR395, which is strongly up-regulated by sulfate deficiency and targets two components of the sulfate uptake and assimilation pathway. Here we show that miR395 levels are affected by treatments with metabolites regulating sulfate assimilation. The precursor of cysteine, O-acetylserine, which accumulates during sulfate deficiency, causes increase in miR395 accumulation. Feeding plants with cysteine, which inhibits sulfate uptake and assimilation, induces miR395 levels while buthionine sulfoximine, an inhibitor of glutathione synthesis, lowers miR395 expression. Thus, miR395 is an integral part of the regulatory network of sulfate assimilation.
authors: John Innes Centre, Norwich Research Park, Norwich, UK.
authors: Colette A
authors: CA
authors: Matthewman
authors: Cintia G
authors: CG
authors: Kawashima
authors: Dalibor
authors: D
authors: Húska
authors: Tibor
authors: T
authors: Csorba
authors: Tamas
authors: T
authors: Dalmay
authors: Stanislav
authors: S
authors: Kopriva
mesh_terms: D027321:Anion Transport Proteins / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D003545:Cysteine / Q000378:metabolism / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D053858:Metabolic Networks and Pathways / Q000187:drug effects / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism*; D012694:Serine / Q000031:analogs & derivatives / Q000494:pharmacology; D013431:Sulfates / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D027321:Anion Transport Proteins; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C500268:EIL3 protein, Arabidopsis; D035683:MicroRNAs; C534518:PS1 protein, Arabidopsis; D018749:RNA, Plant; D013431:Sulfates; D014157:Transcription Factors; D012694:Serine; D019328:Buthionine Sulfoximine; C043943:O-acetylserine; D003545:Cysteine