title: Philadelphia chromosome detection in chronic myeloid leukemia: utility of phytohemagglutinin-stimulated peripheral blood culture.
abstract: BACKGROUND
  The conventional cytogenetic approach to demonstrate Philadelphia (Ph) chromosome at times does not yield enough number of metaphases or are of suboptimal quality. Further, the rapid molecular tests have completely pushed this simple technique into disrepute.
  
  
  OBJECTIVE
  This study aimed to evaluate usefulness of phytohemagglutinin (PHA)-stimulated peripheral blood culture for detection of Ph chromosome in chronic myeloid leukemia (CML) patients.
  
  
  METHODS
  Fifty-six patients, including 11 newly diagnosed cases of CML and 45 patients of CML on imatinib therapy showing the presence of Ph chromosome in unstimulated samples, were included in the study. Cytogenetic analysis was done on unstimulated samples, i.e. bone marrow aspirate, 24- and 48-h peripheral blood culture, and compared with PHA-stimulated 72-h peripheral blood culture.
  
  
  RESULTS
  The preparations from PHA-stimulated peripheral blood culture samples in all 56 patients yielded high number of good-quality metaphases. All the 11 (100%) newly diagnosed patients and 39/45 (87%) of the patients on imatinib therapy showed the presence of Ph chromosome in PHA-stimulated samples. Addition of PHA-stimulated 72-h peripheral blood culture preparation can be of use for increasing the diagnostic yield in cases of CML with suboptimal results on conventional cytogenetics from bone marrow aspirate sample.
authors: Department of Hematology, Postgraduate Institute of Medical Education & Research, Chandigarh, Punjab, India.
authors: Man Updesh Singh
authors: MU
authors: Sachdeva
authors: Neelam
authors: N
authors: Varma
authors: Kamer Singh
authors: KS
authors: Rana
authors: Subhash
authors: S
authors: Varma
mesh_terms: D000328:Adult; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000473:pathology*; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome; D010835:Phytohemagglutinins / Q000378:metabolism
chemical_list: D010835:Phytohemagglutinins