title: Preparation of stable Pd nanocubes and their use in biological labeling.
abstract: Stable Pd nanocubes (PdNC) with the average size ~15 nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres.
authors: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic. slouf@imc.cas.cz
authors: Miroslav
authors: M
authors: Slouf
authors: Martin
authors: M
authors: Hruby
authors: Zulfiya
authors: Z
authors: Bakaeva
authors: Helena
authors: H
authors: Vlkova
authors: Jana
authors: J
authors: Nebesarova
authors: Anatoly A
authors: AA
authors: Philimonenko
authors: Pavel
authors: P
authors: Hozak
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry; D001965:Bromides / Q000737:chemistry; D003102:Colloids; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018796:Immunoconjugates / Q000737:chemistry; D007136:Immunoglobulins / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008867:Microtomy; D009687:Nuclear Proteins / Q000032:analysis; D000090243:Nucleophosmin; D010165:Palladium / Q000737:chemistry*; D010316:Particle Size; D017680:Potassium Compounds / Q000737:chemistry; D011205:Povidone / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*; D014867:Water
chemical_list: D001965:Bromides; D003102:Colloids; D018796:Immunoconjugates; D007136:Immunoglobulins; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D017680:Potassium Compounds; D014867:Water; D000090243:Nucleophosmin; C030174:tetrachloropalladium; D010165:Palladium; D011205:Povidone; C039004:potassium bromide; D001205:Ascorbic Acid