title: The relationship between the variants of the bovine MBL2 gene and milk production traits, mastitis, serum MBL-C levels and complement activity.
abstract: Mannose-binding lectin (MBL), a calcium-dependent collagenous lectin, plays an important role in the host immune defence against a wide range of pathogens. There are MBL1 and MBL2 genes which encode the MBL-A and MBL-C proteins, respectively. This study was carried out to investigate the relationship between the variants of the bovine MBL2 gene and milk production traits, mastitis, serum MBL-C levels and hemolytic complement activity in both classical pathway (CH50) and alternative pathway (ACH50) in Chinese Holstein cattle. Four single-nucleotide polymorphisms (SNPs) in the exon 1 of the MBL2 gene in Chinese Holstein cattle and Luxi yellow cattle were identified by the direct sequencing method. The SNP g.201 G>A was identified as a non-synonymous mutation (codon 31, Arg>Gln) at the N-terminus cysteine-rich domain and the SNPs g.234 C>A and g.235 G>A (codon 42) made Pro to Gln at the 1st Gly-X-Y repeat of the collagen-like domain, while the SNP g.244 T>C (codon 45) was identified as a synonymous mutation (Asn>Asn) at the 2 th Gly-X-Y repeat of the collagen-like domain. The SNP markers (g.201 G>A, and g.234 C>A) were significantly correlated with somatic cell score (SCS) (P<0.05). The concentration of MBL-C protein in serum ranges from 0.8 to 7.4 μg/mL by enzyme-linked immunosorbent assay. Six combinations of different haplotypes from the four SNPs were identified in Chinese Holstein cattle. Statistical analysis revealed that cows with the haplotype combination H4H5 exhibited the lowest SCS. The CH50 value of H4H5 and H5H5 cow are significantly higher than H2H5 haplotype combination (P<0.05). The association analysis results showed that the haplotype combination H4H5 may be used as a tolerance haplotype combination for the bovine mastitis.
authors: Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan 250131, PR China.
authors: Xinju
authors: X
authors: Wang
authors: Zhihua
authors: Z
authors: Ju
authors: Jinming
authors: J
authors: Huang
authors: Minghai
authors: M
authors: Hou
authors: Lei
authors: L
authors: Zhou
authors: Chao
authors: C
authors: Qi
authors: Yan
authors: Y
authors: Zhang
authors: Qing
authors: Q
authors: Gao
authors: Qing
authors: Q
authors: Pan
authors: Guorong
authors: G
authors: Li
authors: Jifeng
authors: J
authors: Zhong
authors: Changfa
authors: C
authors: Wang
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000276:immunology; D003170:Complement Pathway, Alternative / Q000235:genetics* / Q000276:immunology; D003171:Complement Pathway, Classical / Q000235:genetics* / Q000276:immunology; D003165:Complement System Proteins / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005091:Exons / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006239:Haplotypes; D007774:Lactation / Q000235:genetics* / Q000502:physiology; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics* / Q000502:physiology; D008414:Mastitis, Bovine / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D037601:Mannose-Binding Lectin; D003165:Complement System Proteins