title: General biochemical and immunological characteristics of the venom from Peruvian scorpion Hadruroides lunatus.
abstract: This communication describes the general biochemical properties and some immunological characteristics of the venom from the Peruvian scorpion Hadruroides lunatus, which is the most medically relevant species in Peru. The soluble venom of this scorpion is toxic to mice, the LD₅₀ determined was 0.1 mg/kg and 21.55 mg/kg when the venom was injected intracranial or intraperitoneally, respectively. The soluble venom displayed proteolytic, hyaluronidasic, phospholipasic and cardiotoxic activities. High performance liquid chromatography of the soluble venom resulted in the separation of 20 fractions. Two peptides with phospholipasic activity were isolated to homogeneity and their molecular masses determined by mass spectrometry (MALDI TOF). Anti-H. lunatus venom sera were produced in rabbits. Western blotting analysis showed that most of the protein content of this venom is immunogenic. H. lunatus anti-venom displayed consistent cross-reactivity with venom antigens from the new World-scorpions Tityus serrulatus and Centruroides sculpturatus venoms; however, a weaker reactivity was observed against the venom antigens from the old World-scorpion Androctonus australis Hector.
authors: Departamentos de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil.
authors: F
authors: F
authors: Costal-Oliveira
authors: C G
authors: CG
authors: Duarte
authors: R A
authors: RA
authors: Machado de Avila
authors: M M
authors: MM
authors: Melo
authors: K C F
authors: KC
authors: Bordon
authors: E C
authors: EC
authors: Arantes
authors: N C
authors: NC
authors: Paredes
authors: B
authors: B
authors: Tintaya
authors: C
authors: C
authors: Bonilla
authors: R E
authors: RE
authors: Bonilla
authors: W S
authors: WS
authors: Suarez
authors: A
authors: A
authors: Yarleque
authors: J M
authors: JM
authors: Fernandez
authors: E
authors: E
authors: Kalapothakis
authors: Carlos
authors: C
authors: Chávez-Olórtegui
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D007928:Lethal Dose 50; D051379:Mice; D008810:Mice, Inbred C57BL; D010568:Peru; D054467:Phospholipases A2 / Q000378:metabolism; D059748:Proteolysis; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012604:Scorpion Venoms / Q000737:chemistry* / Q000276:immunology* / Q000506:poisoning*; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007106:Immune Sera; D012604:Scorpion Venoms; D054467:Phospholipases A2; D006821:Hyaluronoglucosaminidase