title: Effects of infliximab on bacterial translocation in experimental acute necrotizing pancreatitis.
abstract: OBJECTIVE
  Translocation of bacteria from the gut is an important factor in the development of septic complications and mortality in acute pancreatitis (AP). The present study was designed to assess the effects of infliximab treatment on bacterial translocation (BT) in experimental acute necrotizing pancreatitis.
  
  
  METHODS
  Male Sprague-Dawley rats (n=45) were allocated into three groups. AP was induced in group II (positive control, n=15) and group III (Infliximab; n=15) by retrograde injection of taurocholate into the common biliopancreatic duct. Group I rats (Sham; n=15) received normal saline infusion into the common biliopancreatic duct as placebo. Groups I and II were treated by normal saline and group III was treated with infliximab intraperitoneally on 6, 30 and 54 h after induction of pancreatitis. All surviving animals were killed 60 h after the induction of pancreatitis, and specimens were collected for amylase measurement as well as histopathologic and microbiologic examinations.
  
  
  RESULTS
  Oedema, acinar cell necrosis, inflammatory infiltration, haemorrhage, fat necrosis and perivascular inflammation in group III rats were decreased with infliximab treatment when compared with group II (P<0.001). BT to mesentery lymph node in groups I, II and III were 20, 100 and 46 per cent, respectively. BT to peritoneum and pancreas in group III was lower than group II (P<0.05).
  
  
  CONCLUSIONS
  Infliximab administration resulted in beneficial effects on BT and histopathologic changes in the experimental necrotizing pancreatitis. Whether anti-TNF therapy has a role in prevention of complications of ANP needs to be established.
authors: Department of Surgery, Guven Hospital, A. Ayranci, Ankara, Turkey.
authors: Sezai
authors: S
authors: Aydin
authors: A Turan
authors: AT
authors: Isik
authors: Bulent
authors: B
authors: Unal
authors: Bilgin
authors: B
authors: Comert
authors: Mustafa
authors: M
authors: Ozyurt
authors: Salih
authors: S
authors: Deveci
authors: Gokhan
authors: G
authors: Ozgur
authors: Omer
authors: O
authors: Cengiz
authors: Ilker
authors: I
authors: Tasci
authors: M Refik
authors: MR
authors: Mas
mesh_terms: D061354:Acinar Cells / Q000473:pathology; D000681:Amylases / Q000097:blood; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000009:adverse effects; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D018988:Bacterial Translocation / Q000187:drug effects; D006801:Humans; D000069285:Infliximab; D008297:Male; D023421:Models, Animal; D010183:Pancreatic Ducts / Q000382:microbiology; D019283:Pancreatitis, Acute Necrotizing / Q000139:chemically induced / Q000382:microbiology / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013656:Taurocholic Acid / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D013656:Taurocholic Acid; D000069285:Infliximab; D000681:Amylases