title: Expression, purification, and characterization of a dentin phosphoprotein produced by Escherichia coli, and its odontoblastic differentiation effects on human dental pulp cells.
abstract: To investigate the functions of recombinant human dentin phosphoprotein (rhDPP), we examined cell adhesion, viability and the odontoblastic differentiation activity of human dental pulp cells (hDPCs). Firstly, rhDPP was constructed using pBAD-HisA plasmid in Escherichia coli. Cell adhesion and viability of hDPCs by rhDPP was examined using a crystal violet assay and a MTT assay, ALP, mineralization activity and odontoblastic differentiation-related mRNA levels of hDPCs were measured to elucidate the odontoblastic differentiation effect of rhDPP on hDPCs. Initially, rhDPP significantly and dose-dependently increased hDPCs adhesion versus the untreated control (p < 0.05). Cell viability was also significantly increased by rhDPP at 5 days (p < 0.001). Furthermore, the odontoblastic differentiation effect of rhDPP was verified by measuring ALP activity, mineralization activity and the mRNA levels of odontoblastic differentiation markers. Taken together, rhDPP is expected to play an important role on hDPCs, thereby suggesting its potential use for tooth repair and regeneration.
authors: Institute of Tissue Regeneration Engineering-ITREN, Dankook University, Cheonan 330-714, Republic of Korea.
authors: Ye-Rang
authors: YR
authors: Yun
authors: Eunyi
authors: E
authors: Jeon
authors: Sujin
authors: S
authors: Lee
authors: Wonmo
authors: W
authors: Kang
authors: Sang-Gi
authors: SG
authors: Kim
authors: Hae-Won
authors: HW
authors: Kim
authors: Chang Kook
authors: CK
authors: Suh
authors: Jun-Hyeog
authors: JH
authors: Jang
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D002113:Calcification, Physiologic; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology*; D004926:Escherichia coli; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D009804:Odontoblasts / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human