title: ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis.
abstract: Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.
authors: Department of Plant Systems Biology, VIB, Ghent University, B-9052 Gent, Belgium.
authors: Zhaojun
authors: Z
authors: Ding
authors: Bangjun
authors: B
authors: Wang
authors: Ignacio
authors: I
authors: Moreno
authors: Nikoleta
authors: N
authors: Dupláková
authors: Sibu
authors: S
authors: Simon
authors: Nicola
authors: N
authors: Carraro
authors: Jesica
authors: J
authors: Reemmer
authors: Aleš
authors: A
authors: Pěnčík
authors: Xu
authors: X
authors: Chen
authors: Ricardo
authors: R
authors: Tejos
authors: Petr
authors: P
authors: Skůpa
authors: Stephan
authors: S
authors: Pollmann
authors: Jozef
authors: J
authors: Mravec
authors: Jan
authors: J
authors: Petrášek
authors: Eva
authors: E
authors: Zažímalová
authors: David
authors: D
authors: Honys
authors: Jakub
authors: J
authors: Rolčík
authors: Angus
authors: A
authors: Murphy
authors: Ariel
authors: A
authors: Orellana
authors: Markus
authors: M
authors: Geisler
authors: Jiří
authors: J
authors: Friml
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D011058:Pollen / Q000254:growth & development* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids