title: A DNA nanostructure platform for directed assembly of synthetic vaccines.
abstract: Safe and effective vaccines offer the best intervention for disease control. One strategy to maximize vaccine immunogenicity without compromising safety is to rationally design molecular complexes that mimic the natural structure of immunogenic microbes but without the disease-causing components. Here we use highly programmable DNA nanostructures as platforms to assemble a model antigen and CpG adjuvants together into nanoscale complexes with precise control of the valency and spatial arrangement of each element. Our results from immunized mice show that compared to a mixture of antigen and CpG molecules, the assembled antigen-adjuvant-DNA complexes induce strong and long-lasting antibody responses against the antigen without stimulating a reaction to the DNA nanostructure itself. This result demonstrates the potential of DNA nanostructures to serve as general platforms for the rational design and construction of a variety of vaccines.
authors: Center for Single Molecule Biophysics, The Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
authors: Xiaowei
authors: X
authors: Liu
authors: Yang
authors: Y
authors: Xu
authors: Tao
authors: T
authors: Yu
authors: Craig
authors: C
authors: Clifford
authors: Yan
authors: Y
authors: Liu
authors: Hao
authors: H
authors: Yan
authors: Yung
authors: Y
authors: Chang
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000276:immunology; D003713:Dendritic Cells; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D049329:Nanostructures / Q000737:chemistry*; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000737:chemistry* / Q000276:immunology
chemical_list: D014614:Vaccines, Synthetic; D004247:DNA