title: Vaccenic acid-mediated reduction in cytokine production is independent of c9,t11-CLA in human peripheral blood mononuclear cells.
abstract: The ruminant trans fatty acid vaccenic acid (tVA) favorably alters markers of inflammation. However, it is not yet clear whether these effects are attributed to its endogenous partial conversion to c9,t11-CLA, which is known to possess anti-inflammatory properties. We compared the cytokine reducing potential of tVA to c9,t11-CLA in human T-helper (Th) cells as a main source of cytokine production during inflammation. Secondly, we assessed whether a bioconversion of tVA to c9,t11-CLA via stearoyl-CoA desaturase (SCD) encoded activity takes place in peripheral blood mononuclear cells (PBMC) in order to relate the outcomes of intracellular cytokine measurement to the degree of conversion. TVA reduced the percentage of both IL-2 and TNF-α expressing Th cells significantly, but to a lesser extent compared to c9,t11-CLA, as determined by flow cytometry after alloreactive stimulation of PBMC. Pre-treatment with the selective PPARγ antagonist T0070907 largely re-established the IL-2 and TNF-α positive Th cell population in both tVA and c9,t11-CLA treated cultures. Interestingly, while the portion of tVA dose-dependently increased within the cellular lipid fraction, the initially marginal amount of c9,t11-CLA remained unaltered. However, SCD mRNA although abundantly expressed in PBMC was not regulated by tVA. Conclusively, these results suggest that the cytokine reducing effect of tVA in human T cells is independent of c9,t11-CLA, since no bioconversion occurred. Moreover, the data provide evidence that tVA mechanistically acts in a manner similar to c9,t11-CLA.
authors: Department of Nutritional Physiology, Institute of Nutrition, Friedrich Schiller University of Jena, Dornburger Str. 24, D-07743 Jena, Germany. anke.jaudszus@mri.bund.de
authors: Anke
authors: A
authors: Jaudszus
authors: Gerhard
authors: G
authors: Jahreis
authors: Wiebke
authors: W
authors: Schlörmann
authors: Janine
authors: J
authors: Fischer
authors: Ronny
authors: R
authors: Kramer
authors: Christian
authors: C
authors: Degen
authors: Carsten
authors: C
authors: Rohrer
authors: Alexander
authors: A
authors: Roth
authors: Holger
authors: H
authors: Gabriel
authors: Dagmar
authors: D
authors: Barz
authors: Michael
authors: M
authors: Gruen
mesh_terms: D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D009829:Oleic Acids / Q000494:pharmacology*; D047495:PPAR gamma / Q000502:physiology; D013230:Stearoyl-CoA Desaturase / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D007376:Interleukin-2; D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; D047495:PPAR gamma; D014409:Tumor Necrosis Factor-alpha; C523776:SCD1 protein, human; D013230:Stearoyl-CoA Desaturase; C050413:11-octadecenoic acid