title: The identity of the cell adhesive protein substrate affects the efficiency of adeno-associated virus reverse transduction.
abstract: Delivering genes from surfaces, called substrate-mediated gene delivery or reverse transduction, is a useful method to achieve spatial localization of gene delivery. We tested the compatibility of adeno-associated virus (AAV) vectors with various cell adhesive proteins to mediate gene delivery from surfaces. Our studies demonstrate that AAV vectors can be successfully adsorbed on collagen I, elastin, and laminin substrates leading to robust gene delivery to overlying cells. Notably, AAV immobilization on laminin yields the highest efficiency of gene expression. This increased gene expression cannot be explained by increases in the levels of virus deposition, transcriptional activity of cells, or virus vector uptake into cells. Further refinement of our knowledge of AAV interactions with extracellular matrix proteins may have important implications in a variety of applications ranging from tissue engineering to in vivo gene therapy.
authors: Department of Bioengineering, 6100 Main Street, MS-142, Houston, TX 77005, USA.
authors: Kellie I
authors: KI
authors: McConnell
authors: Eric J
authors: EJ
authors: Gomez
authors: Junghae
authors: J
authors: Suh
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D024042:Collagen Type I / Q000494:pharmacology; D000229:Dependovirus / Q000187:drug effects* / Q000378:metabolism*; D004549:Elastin / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007797:Laminin / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014161:Transduction, Genetic
chemical_list: D015815:Cell Adhesion Molecules; D024042:Collagen Type I; D007797:Laminin; D049452:Green Fluorescent Proteins; D004549:Elastin