title: GM-CSF controls nonlymphoid tissue dendritic cell homeostasis but is dispensable for the differentiation of inflammatory dendritic cells.
abstract: GM-CSF (Csf-2) is a critical cytokine for the in vitro generation of dendritic cells (DCs) and is thought to control the development of inflammatory DCs and resident CD103(+) DCs in some tissues. Here we showed that in contrast to the current understanding, Csf-2 receptor acts in the steady state to promote the survival and homeostasis of nonlymphoid tissue-resident CD103(+) and CD11b(+) DCs. Absence of Csf-2 receptor on lung DCs abrogated the induction of CD8(+) T cell immunity after immunization with particulate antigens. In contrast, Csf-2 receptor was dispensable for the differentiation and innate function of inflammatory DCs during acute injuries. Instead, inflammatory DCs required Csf-1 receptor for their development. Thus, Csf-2 is important in vaccine-induced CD8(+) T cell immunity through the regulation of nonlymphoid tissue DC homeostasis rather than control of inflammatory DCs in vivo.
authors: Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA.
authors: Melanie
authors: M
authors: Greter
authors: Julie
authors: J
authors: Helft
authors: Andrew
authors: A
authors: Chow
authors: Daigo
authors: D
authors: Hashimoto
authors: Arthur
authors: A
authors: Mortha
authors: Judith
authors: J
authors: Agudo-Cantero
authors: Milena
authors: M
authors: Bogunovic
authors: Emmanuel L
authors: EL
authors: Gautier
authors: Jennifer
authors: J
authors: Miller
authors: Marylene
authors: M
authors: Leboeuf
authors: Geming
authors: G
authors: Lu
authors: Costica
authors: C
authors: Aloman
authors: Brian D
authors: BD
authors: Brown
authors: Jeffrey W
authors: JW
authors: Pollard
authors: Huabao
authors: H
authors: Xiong
authors: Gwendalyn J
authors: GJ
authors: Randolph
authors: Jerry E
authors: JE
authors: Chipuk
authors: Paul S
authors: PS
authors: Frenette
authors: Miriam
authors: M
authors: Merad
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation; D019070:Cell Lineage; D053648:Cytokine Receptor Common beta Subunit / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D003713:Dendritic Cells / Q000145:classification / Q000166:cytology / Q000276:immunology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology; D019446:Endotoxemia / Q000276:immunology; D020869:Gene Expression Profiling; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D006706:Homeostasis; D007249:Inflammation / Q000276:immunology*; D008070:Lipopolysaccharides / Q000633:toxicity; D008088:Listeriosis / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009000:Monocytes / Q000637:transplantation; D009928:Organ Specificity; D009976:Orthomyxoviridae Infections / Q000276:immunology; D011008:Pneumococcal Infections / Q000276:immunology; D011828:Radiation Chimera; D013154:Spleen / Q000276:immunology; D013629:Tamoxifen / Q000494:pharmacology
chemical_list: D053648:Cytokine Receptor Common beta Subunit; D008070:Lipopolysaccharides; C440499:lipopolysaccharide, Escherichia coli O111 B4; D013629:Tamoxifen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor