title: Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation.
abstract: Acute graft-vs-host disease (aGVHD) is a frequent, life-threatening complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Despite that, there are no reliable molecular markers reflecting the onset or clinical course of aGVHD. We performed a pilot study on gene expression profiling in peripheral blood mononuclear cells taken from 15 patients with hematological malignancies who underwent allo-HSCT and developed aGVHD. Based on survival rates after aGVHD, patients were divided into two groups-favorable (all patients alive; median follow-up 40 months) vs unfavorable group (all patients died; median survival 2 months). Two-hundred and eighty genes differentially expressed between these two groups were identified; among them, genes responsible for cytokine signaling, inflammatory response, and regulation of cell cycle were over-represented; interleukin-8, G0S2, ANXA3, and NR4A2 were upregulated in the unfavorable group, CDKN1C was downregulated in the same group. Interestingly, the same genes were also described as overexpressed in connection with autoimmune diseases. This indicates an involvement of similar immune regulatory pathways also in aGVHD. Our data support use of gene expression profiling at aGVHD onset for a prediction of its outcomes.
authors: Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
authors: Jan
authors: J
authors: Verner
authors: Jitka
authors: J
authors: Kabathova
authors: Alexandra
authors: A
authors: Tomancova
authors: Sarka
authors: S
authors: Pavlova
authors: Boris
authors: B
authors: Tichy
authors: Marek
authors: M
authors: Mraz
authors: Yvona
authors: Y
authors: Brychtova
authors: Marta
authors: M
authors: Krejci
authors: Zbynek
authors: Z
authors: Zdrahal
authors: Martin
authors: M
authors: Trbusek
authors: Jana
authors: J
authors: Volejnikova
authors: Petr
authors: P
authors: Sedlacek
authors: Michael
authors: M
authors: Doubek
authors: Jiri
authors: J
authors: Mayer
authors: Sarka
authors: S
authors: Pospisilova
mesh_terms: D000208:Acute Disease; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling; D006086:Graft vs Host Disease / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D015996:Survival Rate