title: Label-free highly sensitive detection of telomerase activity in cancer cell by chemiluminescence imaging.
abstract: We have developed a new methodology for label-free highly sensitive telomerase activity assay using chemiluminescence imaging. This method can detect the telomerase activity from as little as 10 cultured cancer cells without PCR. Furthermore, telomerase inhibition is shown, demonstrating the potential for screening of telomerase inhibitors as anticancer drug agents.
authors: School of Biological Science and Technology, Central South University, Changsha 410013, China. lhlmcb@yahoo.com.cn
authors: Changbei
authors: C
authors: Ma
authors: Kun
authors: K
authors: Xia
authors: Hanchun
authors: H
authors: Chen
authors: Weimin
authors: W
authors: Zeng
authors: Rui
authors: R
authors: Han
authors: Jianhua
authors: J
authors: Tang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D049449:Luminescence; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D009369:Neoplasms / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019098:Telomerase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D015215:Zidovudine / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D015215:Zidovudine; D019098:Telomerase