title: Extraction and radicals scavenging activity of polysaccharides with microwave extraction from mung bean hulls.
abstract: Extraction variables of microwave extraction mung polysaccharides (MEMPs) from mung bean hulls were optimized by Box-Behnken design. The optimal extraction parameter of MEMP was 700 W, 70 s and 17 mL/g, and highest recovery was 60.03±2.56 mg GE/g DW, which was agreed closely with predicted values. Two purified polysaccharide fractions (MEMP-1 and MEMP-2) were successfully isolated through DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography in steps. MEMP-1 was mainly composed of mannose and galactose while that of MEMP-2 was rhamnose and galactose. MEMP and purified fractions both had great radicals scavenging activities of hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and MEMP-2 possessed highest antioxidant activity. The results suggested that MEMP-2 could be the suitable natural antioxidants and may be the functional foods for humans.
authors: Institute of Agro-Food Science & Technology, Chinese Academy of Agricultural Science, Beijing 100193, China.
authors: Kui
authors: K
authors: Zhong
authors: Weijing
authors: W
authors: Lin
authors: Qiang
authors: Q
authors: Wang
authors: Sumei
authors: S
authors: Zhou
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification; D017665:Hydroxyl Radical / Q000737:chemistry; D008872:Microwaves; D015233:Models, Statistical; D010851:Picrates / Q000737:chemistry; D019441:Plant Epidermis / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D015203:Reproducibility of Results
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl