title: Component-resolved diagnosis with commercially available D. pteronyssinus Der p 1, Der p 2 and Der p 10: relevant markers for house dust mite allergy.
abstract: OBJECTIVE
  To establish the prevalence and serum levels of IgE to commercial Der p 1, Der p 2, Der p 10 and the carbohydrate MUXF3 in house dust-mite allergic patients. To compare individual vs. allergen microarray methods.
  
  
  METHODS
  Prevalence and serum levels of IgE to Dermatophagoides pteronyssinus extract and components Der p 1, Der p 2, Der p 10 and MUXF3, specific IgG4 to D. pteronyssinus, total serum IgE levels, and clinical features (age, asthma, rhinitis and atopic dermatitis) were determined in 123 patients (64 children) with the ImmunoCAP® method. ImmunoCAP ISAC® was performed in 24 patients.
  
  
  RESULTS
  All patients had serum IgE to D. pteronyssinus. Prevalences of serum IgE to commercial components were Der p 1 93%, Der p 2 77% (Der p 1 or Der p 2 94%), Der p 10 28% and MUXF3 25%. Levels of D. pteronyssinus IgE strongly correlated with Der p 1 and Der p 2 IgE (r = 0.89 and 0.85 respectively), but not Der p 10 and MUXF3. ImmunoCAP® and ImmunoCAP ISAC® were concordant, but the quantitative correlation was poor. No clinical implication for the prevalence, levels, or molecular IgE reactivity profile to house dust mite components was found.
  
  
  CONCLUSIONS
  Commercially available Der p 1 and Der p 2 strongly correlate with IgE D. pteronyssinus. The lack of Der p 1 and Der p 2 IgE may help with differential diagnosis. Der p 10 serum IgE prevalence and levels suggest different patterns in food and mite-related tropomyosin sensitization. Serum IgE to carbohydrate MUXF3, although unexpectedly prevalent, were low and did not modify D. pteronyssinus IgE levels. Follow-up may be best carried out with individual rather than microarrayed components.
authors: Laboratoire d’Immunologie, Hôpital Conception 147 bd Baille F-13005 Marseille, France.
authors: M
authors: M
authors: Bronnert
authors: J
authors: J
authors: Mancini
authors: J
authors: J
authors: Birnbaum
authors: C
authors: C
authors: Agabriel
authors: V
authors: V
authors: Liabeuf
authors: F
authors: F
authors: Porri
authors: I
authors: I
authors: Cleach
authors: A
authors: A
authors: Fabre
authors: I
authors: I
authors: Deneux
authors: V
authors: V
authors: Grandné
authors: J-J
authors: JJ
authors: Grob
authors: P
authors: P
authors: Berbis
authors: D
authors: D
authors: Charpin
authors: P
authors: P
authors: Bongrand
authors: J
authors: J
authors: Vitte
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D060829:Arthropod Proteins / Q000276:immunology*; D001249:Asthma / Q000175:diagnosis / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003546:Cysteine Endopeptidases / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000175:diagnosis / Q000276:immunology; D040002:Dermatophagoides pteronyssinus / Q000276:immunology; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D039981:Pyroglyphidae / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D012221:Rhinitis, Allergic, Perennial / Q000175:diagnosis / Q000276:immunology; D014335:Tropomyosin / Q000276:immunology*; D055815:Young Adult
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451163:Dermatophagoides pteronyssinus antigen p 2; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic; D014335:Tropomyosin; C111884:der p 10 protein, Dermatophagoides pteronyssinus; D007073:Immunoglobulin E; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1