title: Dielectric behaviour and conformational stability of collagen on interaction with DNA.
abstract: Collagen-DNA interaction studies will aid in improving the stability of DNA against nucleases. In the present study, the effect of DNA on different physico-chemical properties of collagen like viscosity, conformation and dielectric behaviour has been studied. Increase of DNA concentration leads to the increment of viscosity of collagen at the pH 4 and 5, but the trend is reversed at the pH of 6 and 7 due to the formation of collagen fibrils. The temperature dependent CD spectroscopic studies for collagen-DNA conjugate showed that thermal stability of collagen is modulated with increasing molar concentration of DNA. It also shows that DNA interactions with collagen did not result in change in the triple helical structure of collagen. Impedance measurements show that the strength of ion pairs for different molar concentrations of collagen-DNA conjugates has changed. Nyquist plot for collagen-DNA conjugate posses higher Y″ at DNA concentration of 1:25 and 1:50 whereas at 1:1 and 1:10 lower Y″ than the native collagen have been observed. An understanding of this nature of the collagen-DNA interactions is helpful for gene delivery applications.
authors: Chemical Laboratory, Central Leather Research Institute, Council of Scientific and Industrial Research, Adyar, Chennai 600020, India.
authors: Aswathy
authors: A
authors: Kandamchira
authors: Ivy
authors: I
authors: Kanungo
authors: Nishter Nishad
authors: NN
authors: Fathima
mesh_terms: D000818:Animals; D002417:Cattle; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D017097:Electric Impedance; D011485:Protein Binding; D011487:Protein Conformation; D055550:Protein Stability; D013696:Temperature; D014783:Viscosity
chemical_list: D003094:Collagen; D004247:DNA