title: Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases--application to feed supplements.
abstract: In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100% aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L(-1) could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.
authors: Department of Chemistry, Division of Analytical Chemistry, University of Natural Resources and Life Sciences-BOKU, Vienna, Austria.
authors: Stefan
authors: S
authors: Neubauer
authors: Ariana
authors: A
authors: Rugova
authors: Dinh Binh
authors: DB
authors: Chu
authors: Hedda
authors: H
authors: Drexler
authors: Anja
authors: A
authors: Ganner
authors: Michael
authors: M
authors: Sauer
authors: Diethard
authors: D
authors: Mattanovich
authors: Stephan
authors: S
authors: Hann
authors: Gunda
authors: G
authors: Koellensperger
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D056148:Chromatography, Reverse-Phase; D045424:Complex Mixtures / Q000737:chemistry; D057230:Limit of Detection; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D010758:Phosphorus / Q000032:analysis; D011687:Purines / Q000032:analysis*; D011743:Pyrimidines / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D015003:Yeasts / Q000737:chemistry*
chemical_list: D045424:Complex Mixtures; D009705:Nucleosides; D009711:Nucleotides; D011687:Purines; D011743:Pyrimidines; D010758:Phosphorus