title: Immunolocalization of podoplanin in benign odontogenic tumours with and without ectomesenchyme.
abstract: OBJECTIVE
  To investigate podoplanin expression in epithelial odontogenic tumours with and without ectomesenchyme and verify the association between its immunoexpression and proliferative activity in keratocystic odontogenic tumours (KCOTS) and orthokeratinized odontogenic cysts (OOCs).
  
  
  METHODS
  Eight ameloblastomas, nine adenomatoid odontogenic tumours, twenty KCOTS, five OOC, one calcifying epithelial odontogenic tumour, two ameloblastic fibromas, four ameloblastic fibro-odontomas and five calcifying cystic odontogenic tumours were immunohistochemically analysed with anti-podoplanin antibody. For KCOTS and OOC, the cell proliferation index was determined with Ki-67 immunostaining and compared by Spearman correlation coefficient.
  
  
  RESULTS
  Podoplanin was expressed in the peripheral odontogenic epithelium of most tumours. Ectomesenchyme was negative, except for odontoblasts. KCOTS exhibited positive podoplanin expression while in OOC it was absent/weak. There was statistically significant correlation (p=0.006) between podoplanin expression and cellular proliferation index of KCOTS and OOC.
  
  
  CONCLUSIONS
  Podoplanin seems to be related to the proliferative activity of KCOTS and may have a role in the process of local invasion of odontogenic tumours with and without ectomesenchyme.
authors: Department of Stomatology, Area of Pathology, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
authors: Adriana dos Santos
authors: Ados S
authors: Caetano
authors: Kellen Cristine
authors: KC
authors: Tjioe
authors: Simone Eloiza Sita
authors: SE
authors: Faustino
authors: João Adolfo Costa
authors: JA
authors: Hanemann
authors: Andréa de Faria Fernandes
authors: Ade F
authors: Belone
authors: Cléverson Teixeira
authors: CT
authors: Soares
authors: Denise Tostes
authors: DT
authors: Oliveira
mesh_terms: D018070:Biological Specimen Banks; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007573:Jaw Neoplasms / Q000378:metabolism* / Q000473:pathology; D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008648:Mesoderm / Q000378:metabolism* / Q000473:pathology; D009807:Odontogenic Cysts / Q000378:metabolism* / Q000473:pathology; D009808:Odontogenic Tumors / Q000378:metabolism* / Q000473:pathology; D000098853:Podoplanin
chemical_list: D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins; C117468:PDPN protein, human; D000098853:Podoplanin