title: Separation of proteins from human plasma by sample displacement chromatography in hydrophobic interaction mode.
abstract: Sample displacement chromatography (SDC) in reversed-phase and ion-exchange modes was introduced approximately 20 years ago. This method was first used for the preparative purification of peptides and proteins. Recently, SDC in ion-exchange mode was also successfully used for enrichment of low-abundance proteins from human plasma. In this paper, the use of SDC for the separation of plasma proteins in hydrophobic interaction mode is demonstrated. By use of two or more columns coupled in series during sample application, and subsequent elution of detached columns in parallel, additional separation of bound proteins was achieved. Further low-abundance, physiologically active proteins could be highly enriched and detected by ESI-MS/MS.
authors: Proteomics Core, COBRE Center for Cancer Research Development, Rhode Island Hospital, Providence, RI, USA. djuro_josic@brown.edu
authors: Djuro
authors: D
authors: Josic
authors: Lucas
authors: L
authors: Breen
authors: James
authors: J
authors: Clifton
authors: Martina Srajer
authors: MS
authors: Gajdosik
authors: Dajana
authors: D
authors: Gaso-Sokac
authors: Marijana
authors: M
authors: Rucevic
authors: Egbert
authors: E
authors: Müller
mesh_terms: D000645:Ammonium Sulfate; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002853:Chromatography, Liquid / Q000379:methods*; D030562:Databases, Protein; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D011108:Polymers; D012965:Sodium Chloride; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001798:Blood Proteins; D011108:Polymers; C089543:Toyopearl Phenyl 650 S; D012965:Sodium Chloride; D000645:Ammonium Sulfate