title: Effectiveness of TaqMan probes for detection of fish eggs and larvae in the stomach contents of a teleost predator.
abstract: Experiments were conducted on the ability of TaqMan molecular probes to detect plaice Pleuronectes platessa DNA from eggs, and cod Gadus morhua DNA from eggs and larvae following ingestion by a teleost predator, whiting Merlangius merlangus. Estimated half-life detection rate (T50) for eggs was 31 h, and 26 h for larvae, with some positive detections occurring even after visual inspection indicated complete gut clearance. Because TaqMan probes are taxon specific, the results presented demonstrate that this technique can provide a means of rapid and unambiguous detection of predation by teleosts on fish eggs and larvae.
authors: Centre for Environment, Fisheries and Aquaculture Science, Pakefield Road, Lowestoft, Suffolk NR33 OHT, U.K. ewan.hunter@cefas.co.uk
authors: E
authors: E
authors: Hunter
authors: N
authors: N
authors: Taylor
authors: C J
authors: CJ
authors: Fox
authors: M
authors: M
authors: Maillard
authors: M I
authors: MI
authors: Taylor
mesh_terms: D000818:Animals; D015342:DNA Probes; D004531:Eggs; D005432:Flounder; D020387:Food Chain; D048388:Gadiformes / Q000502:physiology*; D048389:Gadus morhua; D005766:Gastrointestinal Contents; D007814:Larva; D011235:Predatory Behavior
chemical_list: D015342:DNA Probes