title: Evaluation of antioxidant and free radical scavenging capacities of polyphenolics from pods of Caesalpinia pulcherrima.
abstract: Thirteen polyphenolics were isolated from fresh pods of Caesalpinia pulcherrima using various methods of column chromatography. The structures of these polyphenolics were elucidated as gallic acid (1), methyl gallate (2), 6-O-galloyl-d-glucoside (3), methyl 6-O-galloyl-β-d-glucoside (4), methyl 3,6-di-O-galloyl-α-d-glucopyranoside (5), gentisic acid 5-O-α-d-(6'-O-galloyl)glucopyranoside (6), guaiacylglycerol 4-O-β-d-(6'-O-galloyl)glucopyranoside (7), 3-methoxy-4-hydroxyphenol 1-O-β-d-(6'-O-galloyl) glucopyranoside (8), (+)-gallocatechin (9), (+)-catechin (10), (+)-gallocatechin 3-O-gallate (11), myricetin 3-rhamnoside (12), and ampelopsin (13). All isolated compounds were tested for their antioxidant activities in the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and peroxynitrite radicals scavenging assays. Among those compounds, 11, 12, and 2 exhibited the best DPPH-, hydroxyl-, and peroxynitrite radical-scavenging activities, respectively. Compound 7 is a new compound, and possesses better scavenging activities towards DPPH but has equivalent hydroxyl radical scavenging activity when compared to BHT. The paper is the first report on free radical scavenging properties of components of the fresh pods of Caesalpinia pulcherrima. The results obtained from the current study indicate that the free radical scavenging property of fresh pods of Caesalpinia pulcherrima may be one of the mechanisms by which this herbal medicine is effective in several free radical mediated diseases.
authors: Graduate Institute of Pharmacognosy, School of Pharmacy, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan.|Department of Pharmacy, Taipei Medical University Hospital, 250 Wuxing St., Taipei 11031, Taiwan.
authors: Feng-Lin
authors: FL
authors: Hsu
authors: Graduate Institute of Pharmacognosy, School of Pharmacy, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan.
authors: Wei-Jan
authors: WJ
authors: Huang
authors: School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan.
authors: Tzu-Hua
authors: TH
authors: Wu
authors: Graduate Institute of Pharmacognosy, School of Pharmacy, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan.
authors: Mei-Hsien
authors: MH
authors: Lee
authors: School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan.|Department of Pharmacy, Taipei City Hospital, No.145, Zhengzhou Rd., Taipei 10341, Taiwan.
authors: Lih-Chi
authors: LC
authors: Chen
authors: Graduate Institute of Pharmacognosy, School of Pharmacy, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan.
authors: Hsiao-Jen
authors: HJ
authors: Lu
authors: Graduate Institute of Pharmacognosy, School of Pharmacy, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan.
authors: Wen-Chi
authors: WC
authors: Hou
authors: School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan.
authors: Mei-Hsiang
authors: MH
authors: Lin
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D029911:Caesalpinia / Q000737:chemistry*; D002392:Catechin / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D016166:Free Radical Scavengers / Q000737:chemistry*; D005707:Gallic Acid / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005960:Glucosides; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D005707:Gallic Acid; D002392:Catechin; C004931:1,1-diphenyl-2-picrylhydrazyl