title: Histopathological biomarkers in pacu (Piaractus mesopotamicus) infected with aeromonas hydrophila and treated with antibiotics.
abstract: Pharmacochemicals usage in fish farming disease treatment can cause morphological and functional changes in absorption capacity, metabolism and excretion organs. The aim of this research was to evaluate the histopathological biomarkers in the gills, liver, kidney and skin of pacu (P. mesopotamicus), which have been infected with A. hydrophila and treated with oxytetracycline (OTC) and florfenicol (FFC). Fish were exposed to 2.4×10(7) mL(-1) of A. hydrophila bacteria experimental infection and after 24h exposed to FFC treatment for ten days and OTC for seven days. OTC was not effective in the A. hydrophila control in pacu in up to 170.0 mg kg(-1) concentration. Nevertheless, FFC was 100% effective with 10.0 mg kg(-1) concentration. After the treatment, skin, gills, liver and kidney samples were collected and processed for histopathological analysis. A. hydrophila caused lamellar fusion, sub epithelial edema, mucous hypertrophy and hyperplasia, lining, pillar and chloride cells. Hepatocytes hypertrophy was observed on liver, as a result of the antibiotics metabolism and bacteria cell wall. The histopathological biomarkers show the effects of the presence of the A. hydrophila. The use of the antibiotic florfenicol decreases the bacterial action effectiveness on tissues evaluated. Thus, the histopathological biomarkers show the A. hydrophila effect and the antibiotics treatment. The skin and liver are exposure biomarkers for both.
authors: Aquaculture Center at Unesp, Jaboticabal Campus, São Paulo, Brazil. pacarraschi@yahoo.com.br
authors: Silvia Patrícia
authors: SP
authors: Carraschi
authors: Claudinei
authors: C
authors: da Cruz
authors: Joaquim Gonçalves
authors: JG
authors: Machado Neto
authors: Naiara Fernanda
authors: NF
authors: Ignácio
authors: Roberto
authors: R
authors: Barbuio
authors: Márcia Rita Fernandes
authors: MR
authors: Machado
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D015415:Biomarkers / Q000032:analysis; D060126:Characidae / Q000502:physiology*; D005393:Fish Diseases / Q000473:pathology*; D005880:Gills / Q000382:microbiology / Q000473:pathology; D016905:Gram-Negative Bacterial Infections / Q000473:pathology / Q000662:veterinary*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013839:Thiamphenicol / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D015415:Biomarkers; C035534:florfenicol; D013839:Thiamphenicol