title: Expression of mRNAs for PPT, CGRP, NF-200, and MAP-2 in cocultures of dissociated DRG neurons and skeletal muscle cells in administration of NGF or NT-3.
abstract: Both neurotrophins (NTs) and target skeletal muscle (SKM) cells are essential for the maintenance of the function of neurons and nerve-muscle communication. However, much less is known about the association of target SKM cells with distinct NTs on the expression of mRNAs for preprotachykinin (PPT), calcitonin-gene related peptide (CGRP), neurofilament 200 (NF-200), and microtubule associated protein 2 (MAP-2) in dorsal root ganglion (DRG) sensory neurons. In the present study, a neuromuscular coculture model of dissociated dorsal root ganglion (DRG) neurons and SKM cells was established. The morphology of DRG neurons and SKM cells in coculture was observed with an inverted phase contrast microscope. The effects of nerve growth factor (NGF) or neurotrophin-3 (NT-3) on the expression of mRNAs for PPT, CGRP, NF-200, and MAP-2 was analyzed by real time-PCR assay. The morphology of DRG neuronal cell bodies and SKM cells in neuromuscular coculture at different conditions was similar. The neurons presented evidence of dense neurite outgrowth in the presence of distinct NTs in neuromuscular cocultures. NGF and NT-3 increased mRNA levels of PPT, CGRP, and NF-200, but not MAP-2, in neuromuscular cocultures. These results offer new clues towards a better understanding of the association of target SKM cells with distinct NTs on the expression of mRNAs for PPT, CGRP, NF-200 and MAP-2, and implicate the association of target SKM cells and NTs with DRG sensory neuronal phenotypes.
authors: Department of Anatomy, Shandong University School of Medicine, Jinan, China.
authors: Weiwei
authors: W
authors: Zhang
authors: Hao
authors: H
authors: Li
authors: Ziying
authors: Z
authors: Xing
authors: Hongtu
authors: H
authors: Yuan
authors: Mark S
authors: MS
authors: Kindy
authors: Zhenzhong
authors: Z
authors: Li
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics* / Q000378:metabolism; D048430:Cell Shape / Q000187:drug effects; D018920:Coculture Techniques; D005727:Ganglia, Spinal / Q000166:cytology* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D008858:Microscopy, Phase-Contrast; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D032342:Muscle Cells / Q000187:drug effects / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology*; D020932:Nerve Growth Factor / Q000494:pharmacology; D016900:Neurofilament Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D020933:Neurotrophin 3 / Q000494:pharmacology*; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015320:Tachykinins / Q000235:genetics* / Q000378:metabolism
chemical_list: D008869:Microtubule-Associated Proteins; D016900:Neurofilament Proteins; D020933:Neurotrophin 3; D011498:Protein Precursors; D012333:RNA, Messenger; D015320:Tachykinins; C044206:preprotachykinin; C051774:neurofilament protein H; D020932:Nerve Growth Factor; D015740:Calcitonin Gene-Related Peptide