title: Expression and inhibition of ADAMDEC1 in craniopharyngioma cells.
abstract: OBJECTIVE
  Craniopharyngioma is a common intracranial tumor characterized by high recurrence rate and poor prognosis in spite of multidisciplinary approaches. The ADAM-like decysin 1 (ADAMDEC1) is a member of a disintegrin and metalloprotease (ADAM) family which  correlates with tumor progression and aggressive behavior. This study aimed to detect and inhibit expression of ADAMDEC1 in order to see whether craniopharyngioma cell growth could be suppressed.
  
  
  METHODS
  ADAMDEC1 expression was detected by Western Blot analysis and reverse transcription-polymerase  chain reaction (RT-PCR). Craniopharyngioma cells, which were obtained from tumor samples after surgical removal, were cultured with or without tamoxifen. MTT assay was used to examine tumor cell growth.
  
  
  RESULTS
  ADAMDEC1 mRNA was expressed in craniopharyngioma cell cultures, but  it was not shown in normal brain tissue. Tamoxifen not only reduced ADAMDEC1 mRNA and protein expression, but also inhibited craniopharyngioma cell proliferation.
  
  
  CONCLUSIONS
  ADAMDEC1 may serve as a novel marker for craniopharyngiomas, and tamoxifen could inhibit craniopharyngioma  cell growth and ADAMDEC1 expression.
authors: Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, China. Jianguo_1229@sina.com
authors: Jianguo
authors: J
authors: Xu
authors: Liang
authors: L
authors: Liu
authors: Xiaomei
authors: X
authors: Zheng
authors: Chao
authors: C
authors: You
authors: Qiang
authors: Q
authors: Li
mesh_terms: D051722:ADAM Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D002648:Child; D003397:Craniopharyngioma / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D008875:Middle Aged; D010911:Pituitary Neoplasms / Q000378:metabolism* / Q000473:pathology; D013629:Tamoxifen / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D013629:Tamoxifen; D051722:ADAM Proteins; D008666:Metalloendopeptidases; C108169:decysin