title: Automatic analysis of silver-stained comets by CellProfiler software.
abstract: The comet assay is one of the most widely used methods to evaluate DNA damage and repair in eukaryotic cells. The comets can be measured by software, in a semi-automatic or automatic process. In this paper, we apply the CellProfiler open-source software for automatic analysis of comets from digitized images, reporting the percentage of tail DNA. A side-by-side comparison of CellProfiler with CASP software demonstrated good agreement between the two packages. Our work demonstrates that automatic measurement of silver-stained comets with open-source software is possible, providing significant time savings.
authors: Laboratorio de Radiobiología, Centro de Protección e Higiene de las Radiaciones, La Habana, Cuba. jorgee@cphr.edu.cu
authors: J E
authors: JE
authors: González
authors: I
authors: I
authors: Romero
authors: J F
authors: JF
authors: Barquinero
authors: O
authors: O
authors: García
mesh_terms: D001331:Automation; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D016622:Silver Staining; D012984:Software