title: Accelerated polymer-polymer click conjugation by freeze-thaw treatment.
abstract: Herein, we report a unique technique to accelerate polymer-SNA conjugation based on copper-free click chemistry: gradual freeze-thawing of the reaction solution substantially increases the conjugation rate possibly because of the reactant concentration at the microenvironment scale. This technique was applied to the conjugation between a small interfering RNA (siRNA) and PEG in an aqueous buffer at/below room temperature.
authors: Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-8656, Japan.
authors: Hiroyasu
authors: H
authors: Takemoto
authors: Kanjiro
authors: K
authors: Miyata
authors: Takehiko
authors: T
authors: Ishii
authors: Shota
authors: S
authors: Hattori
authors: Shigehito
authors: S
authors: Osawa
authors: Nobuhiro
authors: N
authors: Nishiyama
authors: Kazunori
authors: K
authors: Kataoka
mesh_terms: D057930:Click Chemistry / Q000379:methods*; D005615:Freezing; D007700:Kinetics; D011092:Polyethylene Glycols / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry*; D012996:Solutions; D044366:Transition Temperature
chemical_list: D034741:RNA, Small Interfering; D012996:Solutions; D011092:Polyethylene Glycols