title: Identification of the actin and plasminogen binding regions of group B streptococcal phosphoglycerate kinase.
abstract: Phosphoglycerate kinase (PGK), present on the surface of group B streptococcus (GBS), has previously been demonstrated to bind the host proteins actin and plasminogen. The actin and plasminogen binding sites of GBS-PGK were identified using truncated GBS-PGK molecules, followed by peptide mapping. These experiments identified two actin and plasminogen binding sites located between amino acids 126-134 and 204-208 of the 398-amino acid-long GBS-PGK molecule. Substitution of the lysine residues within these regions with alanine resulted in significantly reduced binding to both actin and plasminogen. In addition, conversion of the glutamic acid residue at amino acid 133 to proline, the amino acid found at this position for the PGK protein of Streptococcus pneumoniae, also resulted in significantly reduced binding to actin and plasminogen. These results demonstrate that the lysine residues at amino acid positions 126, 127, 130, 204, and 208 along with the glutamic acid residue at amino acid position 133 are necessary for actin and plasminogen binding by GBS-PGK.
authors: Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada.
authors: Tyler J
authors: TJ
authors: Boone
authors: Gregory J
authors: GJ
authors: Tyrrell
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D010449:Peptide Mapping / Q000379:methods; D010735:Phosphoglycerate Kinase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010958:Plasminogen / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D013292:Streptococcus agalactiae / Q000201:enzymology* / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000235:genetics
chemical_list: D000199:Actins; D001426:Bacterial Proteins; D010958:Plasminogen; D010735:Phosphoglycerate Kinase