title: Fhit, E-cadherin, p53, and activation-induced cytidine deaminase expression in endoscopically resected early stage esophageal squamous neoplasia.
abstract: OBJECTIVE
  Abnormal expression of Fragile Histidine Triad (Fhit), E-cadherin and p53 is observed in esophageal squamous cell carcinoma. It has recently been reported that aberrant expression of activation-induced cytidine deaminase (AID) in gastric epithelium leads to the accumulation of nucleotide alterations in the p53 gene. However, little is known about the association between these molecular events and the clinicopathological characteristics of early stage esophageal squamous neoplasia, especially in endoscopically resected tumors.
  
  
  METHODS
  Esophageal squamous neoplasias (n = 49) comprising nine cases of low-grade intraepithelial neoplasia (LGIN), 22 of high-grade intraepithelial neoplasia/carcinoma in situ (HGIN/CIS) and 18 of invasive cancers, were endoscopically resected. Their expression of the tumor-related proteins: Fhit, E-cadherin, p53 and AID was assessed using immunohistochemical methods, and the relationship between protein expression and clinicopathological data was examined.
  
  
  RESULTS
  Reduced or absent Fhit and E-cadherin expression was detected in 22% and 0% of LGIN cases, 73% and 14% of HGIN/CIS cases, and 94% and 61% of invasive cancer cases, respectively, showing progressive increases during neoplastic progression (Fhit: P < 0.01, E-cadherin: P < 0.01). Although p53 and AID were overexpressed in these samples, no change in their expression occurred during neoplastic progression. Moreover, p53 expression was not significantly associated with AID expression.
  
  
  CONCLUSIONS
  These results indicate that a decrease in Fhit and E-cadherin expression could be related to the development and progression of esophageal squamous neoplasia, and that the expression of p53 was independent of aberrant AID expression in the early stage of esophageal carcinogenesis.
authors: Division of Medicine and Clinical Science, Tottori University, Yonago, Japan.
authors: Akihiro
authors: A
authors: Hayashi
authors: Kazuo
authors: K
authors: Yashima
authors: Yohei
authors: Y
authors: Takeda
authors: Shuji
authors: S
authors: Sasaki
authors: Koichiro
authors: K
authors: Kawaguchi
authors: Kenichi
authors: K
authors: Harada
authors: Yoshikazu
authors: Y
authors: Murawaki
authors: Hisao
authors: H
authors: Ito
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D000428:Alcohol Drinking; D015820:Cadherins / Q000378:metabolism; D002278:Carcinoma in Situ / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D016009:Chi-Square Distribution; D003564:Cytidine Deaminase / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism; D012907:Smoking; D018709:Statistics, Nonparametric; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D015820:Cadherins; D003564:Cytidine Deaminase; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D000099170:Bis(5'-adenosyl)triphosphatase