title: Neuroprotective effects of ischemic preconditioning and postconditioning on global brain ischemia in rats through the same effect on inhibition of apoptosis.
abstract: Transient forebrain or global ischemia induces neuronal death in vulnerable CA1 pyramidal cells with many features. A brief period of ischemia, i.e., ischemic preconditioning, or a modified reperfusion such as ischemic postconditioning, can afford robust protection of CA1 neurons against ischemic challenge. Therefore, we investigated the effect of ischemic preconditioning and postconditioning on neural cell apoptosis in rats. The result showed that both ischemic preconditioning and postconditioning may attenuate the neural cell death and DNA fragment in the hippocampal CA1 region. Further western blot study suggested that ischemic preconditioning and postconditioning down-regulates the protein of cleaved caspase-3, caspase-6, caspase-9 and Bax, but up-regulates the protein Bcl-2. These findings suggest that ischemic preconditioning and postconditioning have a neuroprotective role on global brain ischemia in rats through the same effect on inhibition of apoptosis.
authors: Neuroscience Center, Wuxi No. 2 Hospital, Nanjing Medical University, Wuxi 214002, China.
authors: Zhe-Min
authors: ZM
authors: Ding
authors: Department of Neurosurgery, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.
authors: Bing
authors: B
authors: Wu
authors: Department of Neurosurgery, Yuyao People's Hospital, Yuyao 315472, China.
authors: Wei-Qiao
authors: WQ
authors: Zhang
authors: Neuroscience Center, Wuxi No. 2 Hospital, Nanjing Medical University, Wuxi 214002, China.
authors: Xiao-Jie
authors: XJ
authors: Lu
authors: Neuroscience Center, Wuxi No. 2 Hospital, Nanjing Medical University, Wuxi 214002, China.
authors: Yu-Chang
authors: YC
authors: Lin
authors: University of Texas Medical School at Houston, Houston, TX 77030, USA.
authors: Yong-Jian
authors: YJ
authors: Geng
authors: Neuroscience Center, Wuxi No. 2 Hospital, Nanjing Medical University, Wuxi 214002, China.
authors: Yi-Feng
authors: YF
authors: Miao
mesh_terms: D000818:Animals; D017209:Apoptosis; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D056547:CA1 Region, Hippocampal / Q000098:blood supply* / Q000473:pathology; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D057775:Ischemic Postconditioning / Q000379:methods*; D019194:Ischemic Preconditioning / Q000379:methods*; D009474:Neurons / Q000166:cytology* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D020169:Caspases