title: Expansion under hypoxic conditions enhances the chondrogenic potential of equine bone marrow-derived mesenchymal stem cells.
abstract: Bone marrow-derived mesenchymal stem cells (BM-MSCs) are widely used in regenerative medicine in horses. Most of the molecular characterisations of BM-MSCs have been made at 20% O(2), a higher oxygen level than the one surrounding the cells inside the bone marrow. The present work compares the lifespan and the tri-lineage potential of equine BM-MSCs expanded in normoxia (20% O(2)) and hypoxia (5% O(2)). No significant differences were found in long-term cultures for osteogenesis and adipogenesis between normoxic and hypoxic expanded BM-MSCs. An up-regulation of the chondrogenesis-related genes (COL2A1, ACAN, LUM, BGL, and COMP) and an increase of the extracellular sulphated glycosaminoglycan content were found in cells that were expanded under hypoxia. These results suggest that the expansion of BM-MSCs in hypoxic conditions enhances chondrogenesis in equine BM-MSCs.
authors: Laboratorio de Genética Bioquímica LAGENBIO, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain. branera@unizar.es
authors: Beatriz
authors: B
authors: Ranera
authors: Ana Rosa
authors: AR
authors: Remacha
authors: Samuel
authors: S
authors: Álvarez-Arguedas
authors: Tomás
authors: T
authors: Castiella
authors: Francisco José
authors: FJ
authors: Vázquez
authors: Antonio
authors: A
authors: Romero
authors: Pilar
authors: P
authors: Zaragoza
authors: Inmaculada
authors: I
authors: Martín-Burriel
authors: Clementina
authors: C
authors: Rodellar
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D018929:Cell Culture Techniques; D020219:Chondrogenesis / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006736:Horses; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology*; D010100:Oxygen / Q000494:pharmacology*
chemical_list: D010100:Oxygen