title: A fluorescently labeled dendronized polymer-enzyme conjugate carrying multiple copies of two different types of active enzymes.
abstract: A hybrid structure of a synthetic dendronized polymer, two different types of enzymes (superoxide dismutase and horseradish peroxidase), and a fluorescent dye (fluorescein) was synthesized. Thereby, a single polymer chain carried multiple copies of the two enzymes and the fluorescein. The entire attachment chemistry is based on UV/vis-quantifiable bis-aryl hydrazone bond formation that allows direct quantification of bound molecules: 60 superoxide dismutase, 120 horseradish peroxidase, and 20 fluorescein molecules on an average polymer chain of 2000 repeating units. To obtain other enzyme ratios the experimental conditions were altered accordingly. Moreover, it could be shown that both enzymes remained fully active and catalyzed a two-step cascade reaction.
authors: Laboratory of Polymer Chemistry, Department of Materials, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland.
authors: Andrea
authors: A
authors: Grotzky
authors: Thomas
authors: T
authors: Nauser
authors: Huriye
authors: H
authors: Erdogan
authors: A Dieter
authors: AD
authors: Schlüter
authors: Peter
authors: P
authors: Walde
mesh_terms: D002850:Chromatography, Gel; D050091:Dendrimers / Q000737:chemistry*; D019793:Fluorescein / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D050091:Dendrimers; D005456:Fluorescent Dyes; D011108:Polymers; D006735:Horseradish Peroxidase; D013482:Superoxide Dismutase; D019793:Fluorescein