title: In vitro biocompatibility of a platinum-electrode embedded photosensitive polyimide (Durimide) retinal prosthesis.
abstract: OBJECTIVE
  The photosensitive polyimide film, Durimide, is a common component of retinal prostheses; however, retinal cell response to Durimide has not been effectively studied This work assessed the in vitro biocompatibility of a retinal prosthesis containing platinum-electrode embedded Durimide film.
  
  
  METHODS
  Biocompatibility evaluation assessed cytotoxicity, attachment, and proliferation of two cell lines: a human retinal pigmented epithelium cell line (CRL) and a rhesus monkey choroid- retinal endothelial cell line (RF/6A). Cells were cultured with the platinum-electrode embedded Durimide film, with tissue-culture treated polystyrene plates (TCPS) used as a control substrate for cell growth. The effect of a Durimide-exposed medium on cell apoptosis and life cycle was assessed using flow cytometry (FCM).
  
  
  RESULTS
  The indirect cytotoxicity evaluation revealed no toxic effect of the prosthesis on cells. The attachment and proliferation of CRL and RF/6A cells cultured with the Durimide prostheses showed no significant differences to the control. The FCM experiments demonstrated a liquid medium exposed to the prosthesis had no effects on apoptosis or cell life cycle in comparison with the control (p > 0.05).
  
  
  CONCLUSIONS
  The results demonstrate that Durimide has good biocompatibility with retinal cell lines CRL and RF/6A. In conclusion, while further in vitro and in vivo studies are required to clarify long-term effects, Durimide is indicated as a promising material with suitable biocompatibility for retinal implants.
authors: Department of Ophthalmology, Shanghai First People's Hospital Affiliated Shanghai Jiao Tong University, Shanghai, PR China.
authors: Wenjia
authors: W
authors: Liu
authors: ZhengYu
authors: Z
authors: Song
authors: Yanan
authors: Y
authors: Mo
authors: Weijun
authors: W
authors: Wang
authors: Xiaohong
authors: X
authors: Sui
authors: Yuanyuan
authors: Y
authors: Gong
authors: Yuan
authors: Y
authors: Jiang
authors: Lei
authors: L
authors: Zhang
authors: Jiannan
authors: J
authors: Huang
authors: Qing
authors: Q
authors: Gu
authors: Gang
authors: G
authors: Li
authors: Chuanqing
authors: C
authors: Zhou
authors: Zhang
authors: Z
authors: Yu
authors: Qiushi
authors: Q
authors: Ren
authors: Xiaodong
authors: X
authors: Sun
authors: Fenghua
authors: F
authors: Wang
mesh_terms: D017209:Apoptosis; D001672:Biocompatible Materials; D002460:Cell Line; D049109:Cell Proliferation; D004567:Electrodes, Implanted; D005434:Flow Cytometry; D006801:Humans; D008422:Materials Testing; D010984:Platinum; D011474:Prosthesis Design; D057488:Visual Prosthesis
chemical_list: D001672:Biocompatible Materials; D010984:Platinum