title: [The in-vitro effects of arbidol hydrochloride against 2009 new influenza virus A (H1N1)].
abstract: OBJECTIVE
  To detect the in-vitro effects of arbidol hydrochloride against 2009 new influenza virus A (H1N1).
  
  
  METHODS
  The activity of arbidol hydrochloride against 2009 new influenza virus A (H1N1) was determined in MDCK cell cultures. Hemagglutination assay, observation of cytopathic effects, RT-PCR and quantitative RT-PCR tests were performed for determination of virus titers. Inhibition concentration 50% and cytotoxic concentration 50% were calculated with Chou's Menu of Dose-Effect Program.
  
  
  RESULTS
  Arbidol hydrochloride showed low cytotoxicity (cytotoxic concentration 50%>100 μmol/L)and significant anti-2009 new influenza virus A (H1N1) activity in cell cultures. Inhibition concentration 50% were (5.5 ± 0.9), (3.4 ± 0.8), and (1.5 ± 0.2) μmol/L in hemagglutination assay, cytopathic effect test, and quantitative RT-PCR assay, respectively.
  
  
  CONCLUSIONS
  Arbidol has low cytotoxicity and high anti-virus activity and can effectively trigger the activities of interferon and immune response, and therefore can be a valuable anti-influenza virus drug.
authors: University of California, San Diego, CA 92093, USA.
authors: Xing-quan
authors: XQ
authors: Zhang
authors: Jun-peng
authors: JP
authors: Dai
authors: Bai-qiang
authors: BQ
authors: Cai
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004285:Dogs; D007211:Indoles / Q000494:pharmacology*; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects*; D061985:Madin Darby Canine Kidney Cells; D014779:Virus Replication / Q000187:drug effects; D013440:Sulfides
chemical_list: D000998:Antiviral Agents; D007211:Indoles; C086979:umifenovir; D013440:Sulfides