title: MiR-138 suppressed nasopharyngeal carcinoma growth and tumorigenesis by targeting the CCND1 oncogene.
abstract: The microRNA miR-138 is dysregulated in several human cancers, but the underlying mechanism remains largely unknown. Here, we report that miR-138 is commonly underexpressed in nasopharyngeal carcinoma (NPC) specimens and NPC cell lines. The ectopic expression of miR-138 dramatically suppressed cell proliferation and colony formation in vitro and inhibited tumorigenesis in vivo. Moreover, we identified the cyclin D1 (CCND1) gene as a novel direct target of miR-138. In consistent with the knocked-down expression of CCND1, overexpression of miR-138 inhibited cell growth and cell cycle progression in NPC cells. Furthermore, CCND1 was widely upregulated in NPC tumors, and its mRNA levels were inversely correlated with miR-138 expression. Taken together, our findings suggest that miR-138 might be a tumor suppressor in NPC, which is exerted partially by inhibiting CCND1 expression. The identification of functional miR-138 in NPC and its direct link to CCND1 might provide good candidates for developing diagnostic markers and therapeutic applications for NPC.
authors: State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, Guangzhou, China.
authors: Xia
authors: X
authors: Liu
authors: Xiao-Bin
authors: XB
authors: Lv
authors: Xiao-Pai
authors: XP
authors: Wang
authors: Yi
authors: Y
authors: Sang
authors: Shuangbing
authors: S
authors: Xu
authors: Kaishun
authors: K
authors: Hu
authors: Mansi
authors: M
authors: Wu
authors: Yi
authors: Y
authors: Liang
authors: Pan
authors: P
authors: Liu
authors: Jianjun
authors: J
authors: Tang
authors: Wen-Hua
authors: WH
authors: Lu
authors: Qi-Sheng
authors: QS
authors: Feng
authors: Li-Zhen
authors: LZ
authors: Chen
authors: Chao-Nan
authors: CN
authors: Qian
authors: Jin-Xin
authors: JX
authors: Bei
authors: Tiebang
authors: T
authors: Kang
authors: Yi-Xin
authors: YX
authors: Zeng
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D059585:G1 Phase Cell Cycle Checkpoints; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000378:metabolism* / Q000627:therapeutic use; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014183:Transplantation, Heterologous; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; C546110:MIRN138 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D019938:Cyclin D1