title: Protective effect of chromene isolated from Sargassum horneri against UV-A-induced damage in skin dermal fibroblasts.
abstract: Skin homoeostasis is interrupted during UV-A irradiation. How the UV-A-altered skin components influences photoageing of skin should be investigated using human in vitro models that are important for understanding skin ageing. In this study, chromene compound, sargachromenol, was isolated from Sargassum horneri, and its potency on inhibition of photoageing was investigated in UV-A-irradiated dermal fibroblasts. Effects of sargachromenol on the prevention of photoageing were evaluated by measuring ROS production, membrane protein oxidation, lipid peroxidation and ageing-related gene expression in UV-A-irradiated human skin dermal fibroblasts. The results indicated that treatment with sargachromenol suppressed the collagenase matrix metalloproteinases (MMPs), MMP-1, MMP-2 and MMP-9 expression without any cytotoxicity and phototoxicity. It was further found that these inhibitions were because of increase in the expression of TIMP-1 and TIMP-2 genes. Furthermore, we confirmed that the UV-A-induced transcriptions of AP-1 signalling pathway were regulated by sargachromenol treatment in UV-A-irradiated dermal fibroblasts.
authors: Department of Chemistry, Pukyong National University, Busan, Korea.
authors: Jung-Ae
authors: JA
authors: Kim
authors: Byul-Nim
authors: BN
authors: Ahn
authors: Chang-Suk
authors: CS
authors: Kong
authors: Se-Kwon
authors: SK
authors: Kim
mesh_terms: D000287:Administration, Topical; D001578:Benzopyrans / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D045726:Metalloproteases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D044647:Sargassum; D015398:Signal Transduction / Q000187:drug effects; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D015595:Skin Aging / Q000187:drug effects*; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D001578:Benzopyrans; D017382:Reactive Oxygen Species; D019714:Tissue Inhibitor of Metalloproteinases; D018808:Transcription Factor AP-1; C503204:sargachromenol; D045726:Metalloproteases