title: Physicochemical analysis of structural changes in DNA modified with glucose.
abstract: Reactions of reducing sugars with free amino groups of proteins can form advanced glycation end products (AGEs). While the formation of nucleoside AGEs has been studied in detail, no extensive work has been carried out to assess DNA Amadori and DNA advanced glycation end products. In this study, we report biophysical/chemical characterization of glucose-induced changes in DNA, as well as DNA Amadori and DNA advanced glycation end products. Glucose treated DNA exhibited hyperchromicity, decrease in melting temperature, and enhanced emission intensity in a time dependent manner. Formation of DNA Amadori product and DNA advanced glycation end products, mainly CEdG (N²-carboxyethyl-2'-deoxyguanosine), were the major outcome of the study.
authors: Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh 202002, UP, India.
authors: Jalaluddin M
authors: JM
authors: Ashraf
authors: Binish
authors: B
authors: Arif
authors: Kiran
authors: K
authors: Dixit
authors: Moinuddin
authors: Khursheed
authors: K
authors: Alam
mesh_terms: D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D013696:Temperature
chemical_list: D017127:Glycation End Products, Advanced; C400382:N(2)-(1-carboxyethyl)deoxyguanosine; D009580:Nitroblue Tetrazolium; D004247:DNA; D003849:Deoxyguanosine; D005947:Glucose