title: Mitochondrial DNA and Y-chromosome diversity in East Adriatic sheep.
abstract: Variation in mitochondrial DNA (mtDNA) and Y-chromosome haplotypes was analysed in nine domestic sheep breeds (159 rams) and 21 mouflon (Ovis musimon) sampled in the East Adriatic. Mitochondrial DNA analyses revealed a high frequency of type B haplotypes, predominantly in European breeds, and a very low frequency of type A haplotypes, which are more frequent in some Asian breeds. Mitochondrial haplotype Hmt-3 was the most frequent (26.4%), and 37.1%, 20.8% and 7.6% of rams had haplotypes one, two and three mutations remote from Hmt-3 respectively. In contrast, Y-chromosome analyses revealed extraordinary paternal allelic richness: HY-6, 89.3%; HY-8, 5.0%; HY-18, 3.1%; HY-7, 1.3%; and HY-5, 1.3%. In fact, the number of haplotypes observed is comparable to the number found in Turkish breeds and greater than the number found in European breeds so far. Haplotype HY-18 (A-oY1/135-SRYM18), identified here for the first time, provides a link between the haplotype HY-12 (A-oY1/139-SRYM18) found in a few rams in Turkey and haplotype HY-9 (A-oY1/131-SRYM18) found in one ram in Ethiopia. All mouflons had type B mtDNA haplotypes, including the private haplotype (Hmt-55), and all were paternally monomorphic for haplotype HY-6. Our data support a quite homogeneous maternal origin of East Adriatic sheep, which is a characteristic of European breeds. At the same time, the high number of haplotypes found was surprising and intriguing, and it begs for further analysis. Simultaneous analysis of mtDNA and Y-chromosome information allowed us to detect a large discrepancy between maternal and paternal lineages in some populations. This is most likely the result of breeder efforts to 'upgrade' local populations using rams with different paternal origins.
authors: Department of Animal Science, Faculty of Agriculture, University of Zagreb, Svetosimunska 25, Zagreb, Croatia.
authors: M
authors: M
authors: Ferencakovic
authors: I
authors: I
authors: Curik
authors: L
authors: L
authors: Pérez-Pardal
authors: L J
authors: LJ
authors: Royo
authors: V
authors: V
authors: Cubric-Curik
authors: I
authors: I
authors: Fernández
authors: I
authors: I
authors: Alvarez
authors: A
authors: A
authors: Kostelic
authors: N
authors: N
authors: Sprem
authors: K
authors: K
authors: Krapinec
authors: F
authors: F
authors: Goyache
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D017523:Croatia; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D005453:Fluorescence; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012756:Sheep / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial