title: Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: An on-plate testing method using formic acid was evaluated on the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry system using 90 yeast and 78 Corynebacterium species isolates, and 95.6 and 81.1% of yeast and 96.1 and 92.3% of Corynebacterium isolates were correctly identified to the genus and species levels, respectively. The on-plate method using formic acid yielded identification percentages similar to those for the conventional but more laborious tube-based extraction.
authors: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors: Elitza S
authors: ES
authors: Theel
authors: Bryan H
authors: BH
authors: Schmitt
authors: Leslie
authors: L
authors: Hall
authors: Scott A
authors: SA
authors: Cunningham
authors: Robert C
authors: RC
authors: Walchak
authors: Robin
authors: R
authors: Patel
authors: Nancy L
authors: NL
authors: Wengenack
mesh_terms: D003352:Corynebacterium / Q000737:chemistry / Q000302:isolation & purification*; D005561:Formates; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D015003:Yeasts / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D005561:Formates; C030544:formic acid