title: Association between glucan synthesis by streptococcus mutans and caries incidence in schoolchildren receiving a fluoride mouth rinse.
abstract: OBJECTIVE
  To determine whether variation in glucan synthesis by Streptococcus mutans isolates is associated with caries development in children receiving a fluoride mouth rinse (FMR).
  
  
  METHODS
  Of 122 children (aged 9 to 10 years), 64 had received FMR (FMR(+) group) and the remaining 58 children had not (FMR(-) group). The number of decayed and filled teeth (DFT) and increases in the number of DFT in 1 year (dDFT) were recorded. Saliva samples were collected to isolate the clinical S. mutans strains. The isolates were incubated in heart infusion broth supplemented with 1% sucrose, then the amount of water-insoluble glucan (WIG) formed on a glass tube surface was evaluated.
  
  
  RESULTS
  In the FMR(-) group, children carrying S. mutans had a higher DFT (P = 0.039) and tended to have a higher dDFT (P = 0.080) than the others. In the FMR(+) group, although the differences between children with and without S. mutans were not significant, children carrying S. mutans that produced a high amount of WIG had higher dDFT than the other S. mutans-positive children (P = 0.034).
  
  
  CONCLUSIONS
  This study revealed that the variation in glucan synthesis by S. mutans is associated with caries development in children receiving a FMR.
authors: Division on Preventive Dentistry, Department of Oral Health Science, Graduate School of Medical and Dental Sciences, Niigata University, Nigata 951-8514, Japan.
authors: Hidehiro
authors: H
authors: Umetsu
authors: Noboru
authors: N
authors: Kaneko
authors: Akihiro
authors: A
authors: Yoshihara
authors: Shihoko
authors: S
authors: Sakuma
authors: Nobuhiro
authors: N
authors: Hanada
authors: Hideo
authors: H
authors: Miyazaki
mesh_terms: D058491:Bacterial Load; D001431:Bacteriological Techniques; D002327:Cariostatic Agents / Q000627:therapeutic use*; D002648:Child; D004245:DMF Index; D003731:Dental Caries / Q000382:microbiology; D005260:Female; D005459:Fluorides / Q000627:therapeutic use*; D005500:Follow-Up Studies; D005936:Glucans / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D009067:Mouthwashes / Q000627:therapeutic use*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry; D012463:Saliva / Q000382:microbiology; D013295:Streptococcus mutans / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D002327:Cariostatic Agents; D005936:Glucans; D009067:Mouthwashes; D011135:Polysaccharides, Bacterial; D005459:Fluorides