title: Analysis of adipose tissues and stromal vascular cells in a murine arthritis model.
abstract: OBJECTIVE
  Changes in body composition in rheumatoid arthritis (RA), including a reduction in skeletal muscle mass and the accumulation of visceral fat, have been identified, and the interaction between immune abnormality and metabolic disorders has received much attention. The effect of a high-fat (HF) diet and the role of adipose tissue in an arthritis model were investigated.
  
  
  METHODS
  The effect of an HF diet on the histopathology of joints in murine type II collagen-induced arthritis (CIA) was evaluated. The morphology and adipokine production of adipose tissues were analyzed, and macrophages in the stromal vascular fraction (SVF) were counted by flow cytometry. Serum adipokine levels were measured by ELISA.
  
  
  RESULTS
  Significant exacerbation of joint destruction and aggravated pathological conditions were observed in CIA mice that were fed an HF diet. However, the boundary length of adipose tissue tended to decrease and the levels of adipokines (leptin and adiponectin) were lowered by the induction of arthritis. In HF/CIA mice, nevertheless, the production of MCP-1 in adipose tissues and the accumulation of macrophages in the SVF were significantly higher than CON/CIA group. The serum leptin/adiponectin (L/A) ratio was positively correlated with the number of macrophages in the SVF and MCP-1 production by adipose tissue, particularly in the CIA group.
  
  
  CONCLUSIONS
  Functional alterations of adipose tissues could be originated from HF diet during developing arthritis. An abnormal activation of macrophages and an increased production of MCP-1 in adipose tissues might be both involved in joint destruction and inflammation.
authors: The Division of Clinical Nutrition, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan.
authors: Kanae
authors: K
authors: Hamaguchi
authors: Ayako
authors: A
authors: Itabashi
authors: Yuka
authors: Y
authors: Kuroe
authors: Mana
authors: M
authors: Nakano
authors: Eka
authors: E
authors: Fujimoto
authors: Tomomi
authors: T
authors: Kato
authors: Keiko
authors: K
authors: Satoi
authors: Masanori
authors: M
authors: Utsuyama
authors: Kazuto
authors: K
authors: Sato
mesh_terms: D052242:Adiponectin / Q000097:blood; D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D001835:Body Weight; D024043:Collagen Type II; D059305:Diet, High-Fat; D004487:Edema / Q000209:etiology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007249:Inflammation / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000378:metabolism* / Q000473:pathology*; D007596:Joints / Q000378:metabolism / Q000473:pathology*; D020738:Leptin / Q000097:blood; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D012720:Severity of Illness Index; D013154:Spleen / Q000166:cytology; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D052242:Adiponectin; D024043:Collagen Type II; D020738:Leptin