title: Diminished expression of complement regulatory proteins on peripheral blood cells from systemic lupus erythematosus patients.
abstract: CD55, CD59, CD46, and CD35 are proteins with complement regulatory (Creg) properties that ensure cell and tissue integrity when this system is activated. The aim of this study was to evaluate the Creg expression on peripheral blood cells from SLE patients and its association with cytopenia and disease activity. Flow cytometric analyses were performed on blood cells from 100 SLE patients and 61 healthy controls. Compared with healthy controls, we observed in SLE patients with lymphopenia and neutropenia decreased expression of CD55, CD59, and CD46 (P < 0.05). In SLE patients with anemia, CD59 and CD35 were decreased on red blood cells. Furthermore, there was a negative correlation between CD55 and CD59 on neutrophils and the disease activity. The results suggest there is an altered pattern of Creg expression on the peripheral blood cells of SLE patients, and the expression is correlated with disease activity and/or with activation of the complement system.
authors: Serviço de Patologia Clínica, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2350, 2° Andar, 90035-903 Porto Alegre, RS, Brazil. anaalegretti@gmail.com
authors: Ana Paula
authors: AP
authors: Alegretti
authors: Laiana
authors: L
authors: Schneider
authors: Amanda Kirchner
authors: AK
authors: Piccoli
authors: Odirlei Andre
authors: OA
authors: Monticielo
authors: Priscila Schmidt
authors: PS
authors: Lora
authors: João Carlos Tavares
authors: JC
authors: Brenol
authors: Ricardo Machado
authors: RM
authors: Xavier
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D001773:Blood Cells / Q000276:immunology / Q000378:metabolism*; D018958:CD55 Antigens / Q000378:metabolism; D018957:CD59 Antigens / Q000378:metabolism; D003165:Complement System Proteins / Q000276:immunology / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051916:Membrane Cofactor Protein / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D017463:Receptors, Complement 3b / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D018958:CD55 Antigens; D018957:CD59 Antigens; D051916:Membrane Cofactor Protein; D017463:Receptors, Complement 3b; D003165:Complement System Proteins