title: Biomimetic synthesis and characterization of cobalt nanoparticles using apoferritin, and investigation of direct electron transfer of Co(NPs)-ferritin at modified glassy carbon electrode to design a novel nanobiosensor.
abstract: Oxyhydroxy cobalt CoO(OH) nanoparticles (Co-NPs) were prepared in horse spleen apoferritin (HsAFr) cavity. Transmission electron microscopy revealed the particle size was 5.5-6 nm. Mineralization effect on HsAFr was investigated by fluorescence and far-UV circular dichroism (far-UV CD) spectroscopies. The far-UV CD experiments indicated an increase in the α-helical content after mineralization. Intrinsic fluorescence data showed that mineralization acts as a quencher of HsAFr. For the first time, direct electron transfer between Co(NPs)-HsAFr and a glassy carbon electrode in the thin film of dihexadecylphosphate (DHP) was investigated by cyclic voltammetry (CV) to design a biosensor. The anionic surfactant DHP was used to achieve direct electron-transfer between Co(NPs)-HsAFr molecules and the GC electrode surface. CV result showed clearly a pair of well-defined and quasi-reversible redox peaks arise from Co(NPs)-HsAFr embedded in DHP film. This novel biosensor can be used in medical and industrial fields to detect different analytes.
authors: Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Razi University, P.O. Box 67149 Kermanshah, Islamic Republic of Iran. kashanian_s@yahoo.com
authors: Soheila
authors: S
authors: Kashanian
authors: Fereshteh
authors: F
authors: Abasi Tarighat
authors: Ronak
authors: R
authors: Rafipour
authors: Maryam
authors: M
authors: Abbasi-Tarighat
mesh_terms: D000818:Animals; D001052:Apoferritins / Q000737:chemistry*; D032701:Biomimetics; D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D004566:Electrodes; D004583:Electrons; D005293:Ferritins / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D003035:Cobalt; D002244:Carbon; D005293:Ferritins; D001052:Apoferritins