title: [Limb ischemic preconditioning reduces rabbit hepatic ischemia-reperfusion injury through inhibition the phosphorylation of proteins in the MAPK signal pathway in the late phase].
abstract: OBJECTIVE
  To investigate the liver protection mechanisms of MAPK signaling pathway of limb ischemia preconditioning in the late phase.
  
  
  METHODS
  Thirty-six adult male New Zealand white rabbits, weighing 1.8-2.0 kg, were randomly divided equally into 3 groups: group C (sham operation), group L (liver ischemia-reperfusion 24 h after limb ischemia preconditioning), group IR (liver ischemia-reperfusion without limb ischemia preconditioning). Serum alanine transaminase (ALT) was measured during ischemia reperfusion. The tissue and cell injury of liver were examined by optical and electron microscopy. Activation of P38MAPK, P44/P42MAPK, and JNK in hepatic tissue was assessed by western blot after 30 min of reperfusion.
  
  
  RESULTS
  Serum ALT and cell injury in the liver as examined by optical and electron microscopy was decreased in group L as compared with the group IR. Phosphorylation of P38MAPK, P44/ P42MAPK, and JNK were all increased significantly after 30 min of reperfusion. Phosphorylation of P38MAPK and JNK was reduced by limb ischemia pre-treatment.
  
  
  CONCLUSIONS
  Limb ischemia pre-treatment can induce the late phase of preconditioning in rabbit liver through the inhibition of the phosphorylation of P38MAPK and JNK.
authors: Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors: Lijun
authors: L
authors: Cao
authors: Guixiu
authors: G
authors: Yuan
authors: Yaping
authors: Y
authors: Wang
authors: Yetian
authors: Y
authors: Chang
authors: Junmei
authors: J
authors: Xu
authors: Dingquan
authors: D
authors: Zou
authors: Lai
authors: L
authors: Wei
mesh_terms: D000818:Animals; D005121:Extremities / Q000098:blood supply*; D019194:Ischemic Preconditioning / Q000379:methods*; D008099:Liver / Q000098:blood supply*; D020935:MAP Kinase Signaling System; D008297:Male; D010766:Phosphorylation; D011817:Rabbits; D015427:Reperfusion Injury / Q000517:prevention & control*; D048051:p38 Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000502:physiology*
chemical_list: D048051:p38 Mitogen-Activated Protein Kinases