title: Upregulation of miR-650 is correlated with down-regulation of ING4 and progression of hepatocellular carcinoma.
abstract: BACKGROUND
  In the last decade, studies in hepatocellular carcinoma (HCC) demonstrate dysregulation of miRNAs expression. For instance, miR-650 has been implicated in gastric and colorectal cancer tumorigenicity; however, the role of miR-650 remains unknown in HCC.
  
  
  METHODS
  In this study, we performed a comprehensive analysis to examine the miR-650 expression level in 248 HCC and 120 paracarcinomatous liver (PCL) tissues. The correlations between miR-650 expression level and the clinicopathological characteristics (HCC tumorigenicity) were evaluated. The role of miR-650 played in HCC was investigated by Q-PCR, western blot, and MTT.
  
  
  RESULTS
  We found that miR-650 expression was significantly increased in HCC patients and significantly associated with the patients' age (P = 0.0019), differentiation capability (P = 0.0108), and also tumor stage (P = 0.0069). Moreover, we compared the expression level of both ING4 and miR-650 in 122 HCC patients by western blot and real-time PCR. Statistical result showed a significant negative correlation between them (r(s)  = -0.2011, P = 0.0264). Transfection and MTT test suggested that miR-650 decreased the expression of ING4 and stimulate liver cells proliferation significantly.
  
  
  CONCLUSIONS
  These data suggested that miR-650 is correlated with the pathogenesis of HCC and is involved in the HCC tumorigenesis process by inhibiting the expression of ING4.
authors: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors: Zhao-Lin
authors: ZL
authors: Zeng
authors: Fu-Jun
authors: FJ
authors: Li
authors: Feng
authors: F
authors: Gao
authors: Dong-Sheng
authors: DS
authors: Sun
authors: Lei
authors: L
authors: Yao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D015536:Down-Regulation; D005260:Female; D005819:Genetic Markers; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D060888:Real-Time Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D005819:Genetic Markers; D018398:Homeodomain Proteins; C483230:ING4 protein, human; C550124:MIRN650 microRNA, human; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins