title: Glucagon-like peptide-1(1-37) can enhance blood glucose homeostasis in mice.
abstract: Glucagon-like peptide-1 (GLP-1) is produced by the posttranslational processing of proglucagon and acts as a regulator of various homeostatic events. No blood glucose regulation role of GLP-1(1-37) has previously been identified. However, our findings in this study clearly showed that GLP-1(1-37) could lower blood glucose levels both in normal and diabetic mice. In vitro stability analysis demonstrated that GLP-1(1-37) was more stable than GLP-1(7-37), with 94.7% of the initial amount of peptide left after a 4h exposure to mouse serum. Moreover, GLP-1(1-37) was confirmed to be a highly potent agonist of the GLP-1 receptor (GLP-1R) by measuring the expression of the luciferase reporter gene expression in transiently transfected human embryonic kidney (HEK293) cells. Unlike the glucose lowering effect of GLP-1(7-37), the glucose-lowering effect of GLP-1(1-37) could not be blocked by the GLP-1R antagonist exendin(9-39), suggesting that GLP-1(1-37) might activate the GLP-1R via a different mechanism. Therefore, our findings suggest that GLP-1(1-37) could be a potential therapeutic drug for the treatment of type 2 diabetes in the future.
authors: School of Life Science, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, China.
authors: Lifen
authors: L
authors: Zhao
authors: Haifeng
authors: H
authors: Ye
authors: Dongqing
authors: D
authors: Li
authors: Xun
authors: X
authors: Lao
authors: Juan
authors: J
authors: Li
authors: Zhenghua
authors: Z
authors: Wang
authors: Lei
authors: L
authors: Xiao
authors: Zirong
authors: Z
authors: Wu
authors: Jing
authors: J
authors: Huang
mesh_terms: D000818:Animals; D019540:Area Under Curve; D001786:Blood Glucose; D004355:Drug Stability; D004926:Escherichia coli; D005260:Female; D017930:Genes, Reporter; D052216:Glucagon-Like Peptide 1 / Q000008:administration & dosage* / Q000096:biosynthesis / Q000302:isolation & purification; D000067757:Glucagon-Like Peptide-1 Receptor; D005951:Glucose Tolerance Test; D057809:HEK293 Cells; D006706:Homeostasis; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000302:isolation & purification / Q000378:metabolism; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D010446:Peptide Fragments / Q000008:administration & dosage; D018027:Receptors, Glucagon / Q000819:agonists / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000302:isolation & purification
chemical_list: D001786:Blood Glucose; C000593833:GLP1R protein, human; C000593834:Glp1r protein, mouse; D000067757:Glucagon-Like Peptide-1 Receptor; D007004:Hypoglycemic Agents; D010446:Peptide Fragments; D018027:Receptors, Glucagon; D011994:Recombinant Proteins; C083773:exendin (9-39); D052216:Glucagon-Like Peptide 1; D008156:Luciferases