title: Utility of the microculture method in non-invasive samples obtained from an experimental murine model with asymptomatic leishmaniasis.
abstract: The sensitivity of diagnostic methods for visceral leishmaniasis (VL) decreases because of the low number of parasites and antibody amounts in asymptomatic healthy donors who are not suitable for invasive sample acquisition procedures. Therefore, new studies are urgently needed to improve the sensitivity and specificity of the diagnostic approaches in non-invasive samples. In this study, the sensitivity of the microculture method (MCM) was compared with polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and immunofluorescent antibody test (IFAT) methods in an experimental murine model with asymptomatic leishmaniasis. Results showed that the percent of positive samples in ELISA, IFAT, and peripheral blood (PB) -PCR tests were 17.64%, 8.82%, and 5.88%, respectively, whereas 100% positive results were obtained with MCM and MCM-PCR methods. Thus, this study, for the first time, showed that MCM is more sensitive, specific, and economic than other methods, and the sensitivity of PCR that was performed to samples obtained from MCM was higher than sensitivity of the PCR method sampled by PB.
authors: Bioengineering Department, Yildiz Technical University, Istanbul, Turkey. adilmoglu@gmail.com
authors: Adil M
authors: AM
authors: Allahverdiyev
authors: Malahat
authors: M
authors: Bagirova
authors: Rabia
authors: R
authors: Cakir-Koc
authors: Serhat
authors: S
authors: Elcicek
authors: Olga Nehir
authors: ON
authors: Oztel
authors: Sezen
authors: S
authors: Canim-Ates
authors: Emrah Sefik
authors: ES
authors: Abamor
authors: Serap
authors: S
authors: Yesilkir-Baydar
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology; D001483:Base Sequence; D017931:DNA Primers; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007891:Leishmania / Q000276:immunology; D007896:Leishmaniasis / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D017931:DNA Primers