title: Functional expression of the multi-subunit type calcium/proton antiporter from Thermomicrobium roseum.
abstract: Multiple resistance and pH adaptation (Mrp) antiporters are widely distributed in various prokaryotes and have been reported to function as a hetero-oligomeric monovalent cation/proton antiporter, which exchanges a cytoplasmic monovalent cation (Na(+), Li(+), and/or K(+)) with extracellular H(+). In many organisms, they are essential for survival in alkaline or saline environments. Here, we report that the Mrp antiporter from the thermophilic gram-negative bacterium, Thermomicrobium roseum, does not catalyze monovalent cation/proton antiport like the Mrp antiporters studied to date, but catalyzes Ca(2+)/H(+) antiport in Escherichia coli membrane vesicles.
authors: Graduate School of Life Sciences, Toyo University, Oura-gun, Gunma, Japan.
authors: Masato
authors: M
authors: Morino
authors: Masahiro
authors: M
authors: Ito
mesh_terms: D017920:Antiporters / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D041862:Chloroflexi / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression; D009876:Operon; D011522:Protons; D012964:Sodium / Q000378:metabolism
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D011522:Protons; C027614:calcium-hydrogen antiporters; D012964:Sodium; D002118:Calcium