title: Silylated BODIPY dyes and their use in dye-encapsulated silica nanoparticles with switchable emitting wavelengths for cellular imaging.
abstract: Two silylated BODIPY derivatives were synthesized, characterized and used for the fabrication of the dye-encapsulated silica nanoparticles. The fluorophores were covalently incorporated into the silica matrix to minimize any fluorophore leakage. The synthesized fluorophore-doped nanoparticles were stable in aqueous solution and monodisperse with diameters of 20-25 nm. By incorporating the two BODIPY dyes simultaneously at a controlled ratio, silica nanoparticles with switchable emitting wavelengths were achieved with a change in the excitation wavelength. Thus by using the dual-fluorophore-doped nanoparticles, two-color imaging was demonstrated with minimal background signal by employing an appropriate excitation light source and appropriate excitation/emission filter sets. Further, the surfaces of the dual-fluorophore-doped nanoparticles were functionalized with folic acid to allow for the recognition of HeLa cells which over-express the folate receptors.
authors: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, PR China.
authors: Xuehua
authors: X
authors: Hong
authors: Zhuyuan
authors: Z
authors: Wang
authors: Jing
authors: J
authors: Yang
authors: Qingdong
authors: Q
authors: Zheng
authors: Shenfei
authors: S
authors: Zong
authors: Yu
authors: Y
authors: Sheng
authors: Deqin
authors: D
authors: Zhu
authors: Changquan
authors: C
authors: Tang
authors: Yiping
authors: Y
authors: Cui
mesh_terms: D001896:Boron Compounds; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry; D058973:Folate Receptors, GPI-Anchored / Q000378:metabolism; D005492:Folic Acid; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles; D061848:Optical Imaging; D012822:Silicon Dioxide; D013050:Spectrometry, Fluorescence
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D058973:Folate Receptors, GPI-Anchored; D012822:Silicon Dioxide; D005492:Folic Acid