title: Proteome analysis of tunicamycin-induced ER stress.
abstract: Endoplasmic reticulum (ER) stress occurs upon increased levels of unfolded proteins and results in activation of cellular responses such as the unfolded protein response (UPR) and ER-associated protein degradation (ERAD). To examine ER stress, we performed a quantitative proteome analysis of human neuroblastoma cells using stable isotope labeling with amino acids in cell culture (SILAC) in combination with SDS-PAGE and LC-MS/MS. Proteins associated with the ER were overrepresented in the dataset of altered proteins. In particular, ER chaperones responsible for protein folding were significantly upregulated in response to ER stress. The important ER stress regulator 78 kDa glucose-regulated protein (GRP-78 or BiP) was highly upregulated together with several proteins that have been found to form a multiprotein complex with BiP including cyclophilin B, DnaJ homolog subfamily B member 11, endoplasmin, hypoxia upregulated protein 1, protein disulfide isomerase and protein disulfide isomerase A4 upon tunicamycin-induced ER stress. Furthermore, seven aminoacyl-tRNA synthetases and five proteins belonging to the Sec61 complex were increased in response to tunicamycin-induced ER stress.
authors: The Biotechnology Centre of Oslo, University of Oslo, Oslo, Norway.
authors: Vibeke Hervik
authors: VH
authors: Bull
authors: Bernd
authors: B
authors: Thiede
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D006801:Humans; D007553:Isotope Labeling; D009447:Neuroblastoma / Q000378:metabolism; D060066:Protein Interaction Maps; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000145:classification; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014415:Tunicamycin / Q000494:pharmacology*
chemical_list: D011506:Proteins; D020543:Proteome; D014415:Tunicamycin