title: North Western Spain hot springs are a source of lipolytic enzyme-producing thermophilic microorganisms.
abstract: Several hot springs in Galicia (North Western Spain) have been investigated as potential sources of lipolytic enzyme-producing thermophilic microorganisms. After isolating 12 esterase producing strains, 9 of them were assured to be true lipase producers, and consequently grown in submerged cultures, obtaining high extracellular activities by two of them. Furthermore, a preliminary partial characterization of the crude lipase, obtained by ultrafiltration of the cell-free culture supernatant, was carried out at several pH and temperature values. It is outstanding that several enzymes turned out to be multiextremozymes, since they had their optimum temperature and pH at typical values from thermoalkalophiles. The thermal stability in aqueous solution of the crude enzymes was also assayed, and the influence of some potential enzyme stabilizing compounds was tested. Finally, the viability of the selected microorganisms has been demonstrated at bioreactor scale.
authors: Department of Chemical Engineering, University of Vigo, Spain. deive@uvigo.es
authors: Francisco J
authors: FJ
authors: Deive
authors: María S
authors: MS
authors: Alvarez
authors: M Angeles
authors: MA
authors: Sanromán
authors: Maria A
authors: MA
authors: Longo
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D045482:Hot Springs / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D013030:Spain; D013824:Thermus / Q000201:enzymology*; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D008049:Lipase