title: Effect of taurine on IRAK4 and NF-kappa B in Kupffer cells from rat liver grafts after ischemia-reperfusion injury.
abstract: BACKGROUND
  The aim of this study was to explore the protective mechanisms of taurine pretreatment against hepatic ischemia/reperfusion injury after liver transplantation.
  
  
  METHODS
  A Sprague-Dawley-to-Sprague-Dawley rat liver transplantation model was used in this study. At 0, 60, and 180 minutes after reperfusion, expression of interleukin-1 receptor-associated kinase-4 (IRAK-4) messenger ribonucleic acid and protein in Kupffer cells was determined by real-time polymerase chain reaction and Western blotting. The activity of nuclear factor κB in Kupffer cells was determined by electrophoretic mobility shift assay. The serum tumor necrosis factor-α level was detected by enzyme-linked immunosorbent assay. Serum transaminases, liver histology, and animal survival were also investigated.
  
  
  RESULTS
  At 60 and 180 minutes after reperfusion, levels of IRAK-4 messenger ribonucleic acid and protein, activities of nuclear factor κB, and levels of serum transaminases and tumor necrosis factor-α were all obviously elevated. However, changes in these parameters in rats treated with taurine were remarkably attenuated at the indicated time points.
  
  
  CONCLUSIONS
  These data suggest that taurine could protect against hepatic ischemia/reperfusion injury after liver transplantation, and the protective effects may be through downregulation of IRAK-4 and downstream nuclear factor κB and tumor necrosis factor-α expression in Kupffer cells.
authors: Chongqing Key Laboratory of Hepatobliliary Surgery and Department of Hepatobliliary Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors: Ke
authors: K
authors: Sun
authors: Yong
authors: Y
authors: Chen
authors: Shao-yong
authors: SY
authors: Liang
authors: Zuo-jing
authors: ZJ
authors: Liu
authors: Wang-yang
authors: WY
authors: Liao
authors: Zhi-bing
authors: ZB
authors: Ou
authors: Bing
authors: B
authors: Tu
authors: Jian-ping
authors: JP
authors: Gong
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D015153:Blotting, Western; D024202:Electrophoretic Mobility Shift Assay; D004797:Enzyme-Linked Immunosorbent Assay; D015971:Gene Expression Regulation, Enzymologic; D053592:Interleukin-1 Receptor-Associated Kinases / Q000378:metabolism*; D007728:Kupffer Cells / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D008111:Liver Function Tests; D016031:Liver Transplantation / Q000009:adverse effects; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D055031:Primary Graft Dysfunction / Q000209:etiology / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control*; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013654:Taurine / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; D013654:Taurine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D053592:Interleukin-1 Receptor-Associated Kinases