title: Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells.
abstract: OBJECTIVE
  To investigate the protective effects of the natural medicinal monomer isopsoralen (ISR) with estrogenic activity against oxidative damage in human lens epithelial cells B3 (HLE-B3) caused by hydrogen peroxide (H(2)O(2)) and to pursue the possible mitochondrial proteomic regularity of the protective effects.
  
  
  METHODS
  HLE-B3 cells were treated with H(2)O(2) (300 μ mol/L), β-estradiol (E(2): 10(-8) mol/L) and H(2)O(2), ISR (10(-5) mol/L) and H(2)O(2), or left untreated. Altered expressions of all mitochondrial proteins were analyzed by protein array and surfaceenhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS). The mass/charge (m/z) ratios of each peak were tested by the Kruskal-Wallis rank sum test, and the protein peak value of the m/z ratio for each treatment by pair comparison was analyzed with the Nemenyi test.
  
  
  RESULTS
  H(2)O(2) up-regulated the expressions of two protein spots (with m/z of 6532 and 6809). E(2) mitigated the oxidative damage, and the expression of one protein spot (m/z 6532) was down-regulated. In contrast, ISR down-regulated both of protein spots (m/z 6532 and 6809).
  
  
  CONCLUSIONS
  ISR could effectively inhibit H(2)O(2)-induced oxidative damage in HLE-B3 cells. The protein spot at m/z of 6532 might be the target spot of ISR against oxidative damage induced by H(2)O(2).
authors: Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China.
authors: Chun-Yan
authors: CY
authors: Feng
authors: Xiu-Rong
authors: XR
authors: Huang
authors: Ming-Xin
authors: MX
authors: Qi
authors: Song-Wen
authors: SW
authors: Tang
authors: Yan-Hong
authors: YH
authors: Hu
authors: Sheng
authors: S
authors: Chen
authors: Fa-Jie
authors: FJ
authors: Ke
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004958:Estradiol / Q000494:pharmacology; D011564:Furocoumarins / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D007908:Lens, Crystalline / Q000473:pathology*; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D011564:Furocoumarins; D020011:Protective Agents; D020543:Proteome; D004958:Estradiol; D006861:Hydrogen Peroxide; C011659:angelicin