title: In contrast to sheep, goats adapt to dietary calcium restriction by increasing intestinal absorption of calcium.
abstract: Many studies aimed at understanding calcium homeostasis in the cow use sheep or goats as ruminant models. However, the comparability of relevant homeostatic processes between ruminant species has not been assessed. Therefore, we investigated whether the mechanisms of maintaining calcium homeostasis are similar in different ruminant species. Dietary calcium of goats was restricted along with treatment with calcitriol in a similar protocol to that in a recent study with sheep. Plasma calcium and phosphate and parameters of bone metabolism were analysed. Gastrointestinal calcium transport was characterised in vitro in Ussing chambers. The expression of apical epithelial calcium channels, calbindin-D(9K), and the basolateral plasma membrane Ca(2+)-ATPase was determined by quantitative RT-PCR and Western blot analysis. In contrast to sheep, the goats were able to compensate for low dietary calcium supply by increasing active calcium absorption in the small intestine, especially in the jejunum. As in sheep, the observed ruminal calcium transport of goats was affected neither by the calcium restricted diet nor by the calcitriol treatment, thus indicating the presence of an alternative, vitamin D-independent mechanism of calcium transport in the forestomachs. These results demonstrate that mechanisms for maintaining calcium homeostasis differ significantly between ruminant species.
authors: Department of Physiology, University of Veterinary Medicine Hannover, Hannover, Germany. mirja.wilkens@tiho-hannover.de
authors: Mirja R
authors: MR
authors: Wilkens
authors: Julia
authors: J
authors: Richter
authors: David R
authors: DR
authors: Fraser
authors: Annette
authors: A
authors: Liesegang
authors: Gerhard
authors: G
authors: Breves
authors: Bernd
authors: B
authors: Schröder
mesh_terms: D000222:Adaptation, Physiological; D000704:Analysis of Variance; D000818:Animals; D015519:Bone Density; D064026:Calbindins; D002113:Calcification, Physiologic; D002117:Calcitriol / Q000008:administration & dosage; D002118:Calcium / Q000097:blood / Q000172:deficiency; D002136:Calcium, Dietary / Q000378:metabolism*; D004553:Electric Conductivity; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D006041:Goats / Q000378:metabolism* / Q000502:physiology; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism / Q000502:physiology; D008353:Mannitol / Q000378:metabolism; D010710:Phosphates / Q000097:blood; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000235:genetics / Q000378:metabolism; D012756:Sheep / Q000378:metabolism*; D018709:Statistics, Nonparametric; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism; D014815:Vitamins / Q000008:administration & dosage
chemical_list: D064026:Calbindins; D002136:Calcium, Dietary; D010710:Phosphates; D064030:S100 Calcium Binding Protein G; D050916:TRPV Cation Channels; D014815:Vitamins; D008353:Mannitol; D053499:Plasma Membrane Calcium-Transporting ATPases; D002117:Calcitriol; D002118:Calcium