title: Location of T-cell epitopes in nonstructural proteins 9 and 10 of type-II porcine reproductive and respiratory syndrome virus.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant swine pathogen which exhibits considerable sequence diversity. In an attempt to identify highly conserved T-cell epitopes contained in proteins of this virus, we examined heptadecamer peptides spanning the sequence of the PRRSV nonstructural proteins (NSPs) 9 and 10, both of which are highly conserved, for their ability to elicit a recall proliferative and interferon-gamma response in peripheral blood mononuclear cells obtained from pigs immunized against the type-II PRRSV strain FL-12. These studies led to the identification of four peptides, two from each NSP9 and NSP10 that appear to contain T-cell epitopes. Comparison of the amino acid sequence of these four peptide sequences to the analogous sequences from a diverse sample of type-II PRRSV strains indicated that these sequences are highly conserved and thus contain highly conserved T-cell epitopes. The identified epitopes may be important in the formulation of immunogens to provide broad cross-protection against diverse PRRSV strains.
authors: Nebraska Center for Virology, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583-0900, United States.
authors: Rajeshwari
authors: R
authors: Parida
authors: In-Soo
authors: IS
authors: Choi
authors: Daniel A
authors: DA
authors: Peterson
authors: Asit K
authors: AK
authors: Pattnaik
authors: William
authors: W
authors: Laegreid
authors: Federico A
authors: FA
authors: Zuckermann
authors: Fernando A
authors: FA
authors: Osorio
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D017124:Conserved Sequence; D018984:Epitopes, T-Lymphocyte / Q000235:genetics*; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008969:Molecular Sequence Data; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000276:immunology*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013552:Swine; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D007371:Interferon-gamma