title: CTL recognition of HIV-1-infected cells via cross-recognition of multiple overlapping peptides from a single 11-mer Pol sequence.
abstract: It is known that overlapping HIV-1 peptides of different lengths can be presented by a given HLA class I molecule. However, the role of those peptides in CD8(+)  T cells recognition of HIV-1-infected cells remains unclear. Here we investigated the recognition of overlapping 8-mer to 11-mer peptides of Pol 155-165 by HLA-B*54:01-restricted CD8(+)  T cells. The analysis of ex vivo T cells using ELISPOT and tetramer binding assays showed that there were different patterns of CD8(+)  T-cell responses to these peptides among chronically HIV-1-infected HLA-B*54:01(+)  individuals, though the response to the 9-mer peptide was the strongest among them. CD8(+)  T-cell clones with TCRs specific for the 9-mer, 10-mer, and/or 11-mer peptides effectively killed HIV-1-infected cells. Together, these results suggest that the 9-mer and 10-mer peptides could be predominantly presented by HLA-B*54:01, though it remains possible that the 11-mer peptide was also presented by this HLA allele. The present study demonstrates effective CD8(+)  T-cell recognition of HIV-1-infected cells via presentation of multiple overlapping HIV-1 peptides and cross-recognition by the CD8(+)  T cells.
authors: Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
authors: Masao
authors: M
authors: Hashimoto
authors: Tomohiro
authors: T
authors: Akahoshi
authors: Hayato
authors: H
authors: Murakoshi
authors: Naoki
authors: N
authors: Ishizuka
authors: Shinichi
authors: S
authors: Oka
authors: Masafumi
authors: M
authors: Takiguchi
mesh_terms: D017951:Antigen Presentation; D002999:Clone Cells; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D058501:Enzyme-Linked Immunospot Assay; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D015235:HLA-B Antigens / Q000276:immunology; D006801:Humans; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology*; D011485:Protein Binding; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000821:virology; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*
chemical_list: D015235:HLA-B Antigens; C113783:HLA-B54 antigen; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D054302:pol Gene Products, Human Immunodeficiency Virus