title: Expression, purification, crystallization and preliminary X-ray analysis of the D-alanyl carrier protein DltC from Staphylococcus epidermidis.
abstract: The D-alanyl lipoteichoic acids (D-alanyl LTAs) present in the cell walls of Gram-positive bacteria play crucial roles in autolysis, cation homeostasis and biofilm formation. The alanylation of LTAs requires the D-alanyl carrier protein DltC to transfer D-Ala onto a membrane-associated LTA. Here, DltC from Staphylococcus epidermidis (SeDltC) was purified and crystallized using the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.83 Å and belonged to space group P2, with unit-cell parameters a = 66.26, b = 53.28, c = 88.05 Å, β = 98.22°. The results give a preliminary crystallographic analysis of SeDltC and shed light on the functional role of DltC in the alanylation of LTAs.
authors: Department of Biotechnology, Hungkuang University, Taichung 433, Taiwan.
authors: Chi Hung
authors: CH
authors: Huang
authors: Chao Hung
authors: CH
authors: Kao
authors: Chia-Shin
authors: CS
authors: Yang
authors: Chi-Huang
authors: CH
authors: Chang
authors: Sheng-Chia
authors: SC
authors: Chen
authors: Shu-Min
authors: SM
authors: Kuan
authors: Yen-Chao
authors: YC
authors: Su
authors: Yu-Han
authors: YH
authors: Huang
authors: Ming-Chung
authors: MC
authors: Chang
authors: Yeh
authors: Y
authors: Chen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D013212:Staphylococcus epidermidis / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C085651:D-alanyl carrier protein, bacteria