title: NGF promotes long-term memory formation by activating poly(ADP-ribose)polymerase-1.
abstract: Nerve growth factor (NGF) is a critical secreted protein that plays an important role in development, survival, and function of the mammalian nervous system. Previously reports suggest that endogenous NGF is essential for the hippocampal plasticity/memory and NGF deprivation induces the impairment of hippocampus-related memory and synaptic plasticity. However, whether exogenous supplement of NGF could promote the hippocampus-dependent synaptic plasticity/memory and the possible underlying mechanisms are not clear. In this study we found that NGF administration facilitates the hippocampus-dependent long-term memory and synaptic plasticity by increasing the activity of PARP-1, a polymerase mediating the PolyADP-ribosylation and important for the memory formation. Co-application of 3-Aminobenzamide (3-AB), a specific inhibitor of PARP-1, distinctly blocked the boosting effect of NGF on memory and synaptic plasticity, and the activation of downstream PKA-CREB signal pathway. Our data provide the first evidence that NGF supplement facilitates synaptic plasticity and the memory ability through PARP-1-mediated protein polyADP-ribosylation and activation of PKA-CREB pathway.
authors: Department of Pathophysiology, Key Laboratory of Neurological Disease of Education Ministry of China, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
authors: Shao-Hui
authors: SH
authors: Wang
authors: Xiao-Mei
authors: XM
authors: Liao
authors: Dan
authors: D
authors: Liu
authors: Juan
authors: J
authors: Hu
authors: Yang-Yang
authors: YY
authors: Yin
authors: Jian-Zhi
authors: JZ
authors: Wang
authors: Ling-Qiang
authors: LQ
authors: Zhu
mesh_terms: D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001549:Benzamides / Q000494:pharmacology; D015153:Blotting, Western; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D055724:Electrophysiological Phenomena; D004789:Enzyme Activation / Q000187:drug effects; D005239:Fear / Q000523:psychology; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology; D007150:Immunohistochemistry; D017774:Long-Term Potentiation / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D057567:Memory, Long-Term / Q000187:drug effects*; D020932:Nerve Growth Factor / Q000494:pharmacology*; D009473:Neuronal Plasticity / Q000187:drug effects; D010766:Phosphorylation; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects
chemical_list: D001549:Benzamides; D017362:Cyclic AMP Response Element-Binding Protein; C025160:3-aminobenzamide; D020932:Nerve Growth Factor; C499322:Parp1 protein, rat; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D017868:Cyclic AMP-Dependent Protein Kinases