title: An aqueous media based approach for the preparation of a biosensor platform composed of graphene oxide and Pt-black.
abstract: The combination of Pt nanoparticles and graphene was more effective in enhancing biosensing than either nanomaterial alone according to previous reports. Based on the structural similarities between water soluble graphene oxide (GrO(x)) and graphene, we report the fabrication of an aqueous media based GrO(x)/Pt-black nanocomposite for biosensing enhancement. In this approach GrO(x) acted as a nanoscale molecular template for the electrodeposition of Pt-black, an amorphously nanopatterned isoform of platinum metal. Scanning electron microscopy (SEM) images and energy-dispersive X-ray spectroscopy (EDS) showed that Pt-black was growing along GrO(x). The effective surface area and electrocatalytic activity towards H(2)O(2) oxidation of GrO(x)/Pt-black microelectrodes were significantly higher than for Pt-black microelectrodes. When used to prepare a bio-nanocomposite based on protein functionalization with the enzyme glucose oxidase (GOx), the GrO(x)/Pt-black microbiosensors exhibited improved sensitivity over the Pt-black microbiosensors. This suggested that the GrO(x)/Pt-black nanocomposite facilitated an increase in electron transfer, and/or minimized mass transport limitations as compared to Pt-black used alone. Glucose microbiosensors based on GrO(x)/Pt-black exhibited high sensitivity (465.9 ± 48.0 nA/mM), a low detection limit of 1 μM, a linear response range of 1 μM-2mM, and response time of ≈ 4s. Additionally the sensor was stable and highly selective over potential interferents.
authors: Birck-Bindley Physiological Sensing Facility, Purdue University, 1203 West State Street, West Lafayette, IN 47907, USA.
authors: Jin
authors: J
authors: Shi
authors: Hangyu
authors: H
authors: Zhang
authors: Alexandra
authors: A
authors: Snyder
authors: Mei-xian
authors: MX
authors: Wang
authors: Jian
authors: J
authors: Xie
authors: D
authors: D
authors: Marshall Porterfield
authors: Lia A
authors: LA
authors: Stanciu
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D004595:Electroplating; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D008839:Microelectrodes; D053761:Nanocomposites / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D010984:Platinum / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D010087:Oxides; D014867:Water; D010984:Platinum; D006108:Graphite; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose