title: Anti-hyperglycemic effect of chebulagic acid from the fruits of Terminalia chebula Retz.
abstract: In the present study, we firstly compared rat intestinal α-glucosidase inhibitory activity by different ethanol-aqueous extractions from the dried fruits of Terminalia chebula Retz. The enzymatic assay showed that the 80% ethanol extract was more potent against maltase activity than both 50% and 100% ethanol extracts. By HPLC analysis, it was determined that the 80% ethanol extract had a higher content of chebulagic acid than each of 50% or 100% ethanol extract. Next, we investigated how efficiently chebulagic acid could inhibit sugar digestion by determining the glucose level on the apical side of the Caco-2 cell monolayer. The result showed that the maltose-hydrolysis activity was down-regulated by chebulagic acid, which proved to be a reversible inhibitor of maltase in Caco-2 cells. On the other hand, chebulagic acid showed a weak inhibition of sucrose-hydrolysis activity. Meanwhile, chebulagic acid did not have an obvious influence on intestinal glucose uptake and was not effective on glucose transporters. Further animal studies revealed that the oral administration of chebulagic acid (100 mg/kg body weight) significantly reduced postprandial blood glucose levels by 11.1% in maltose-loaded Sprague-Dawley (SD) rats compared with the control group, whereas the oral administration of chebulagic acid did not show a suppressive effect on postprandial hyperglycemia in sucrose- or glucose-loaded SD-rats. The results presented here suggest that chebulagic acid from T. chebula can be used to control blood glucose and manage type 2 diabetes, although clinical trials are needed.
authors: Department of Public Health, Hua Xi Medicinal Center of Sichuan University, Chengdu 610041, China.
authors: Yi-Na
authors: YN
authors: Huang
authors: Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors: Dong-Dong
authors: DD
authors: Zhao
authors: Department of Public Health, Hua Xi Medicinal Center of Sichuan University, Chengdu 610041, China.
authors: Bo
authors: B
authors: Gao
authors: College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China.
authors: Kai
authors: K
authors: Zhong
authors: College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China.
authors: Rui-Xue
authors: RX
authors: Zhu
authors: College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China.
authors: Yan
authors: Y
authors: Zhang
authors: College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China.
authors: Wang-Jun
authors: WJ
authors: Xie
authors: College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China.
authors: Li-Rong
authors: LR
authors: Jia
authors: College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China.
authors: Hong
authors: H
authors: Gao
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001578:Benzopyrans / Q000008:administration & dosage* / Q000494:pharmacology; D018938:Caco-2 Cells; D015536:Down-Regulation; D004353:Drug Evaluation, Preclinical; D005638:Fruit / Q000737:chemistry*; D005960:Glucosides / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D006943:Hyperglycemia / Q000188:drug therapy* / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D029766:Terminalia / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D001578:Benzopyrans; D005960:Glucosides; D007004:Hypoglycemic Agents; D010936:Plant Extracts; C076178:chebulagic acid; D000520:alpha-Glucosidases