title: Protein expression from zooplankton communities in a metal contaminated NW mediterranean coastal ecosystem.
abstract: Bidimensional and monodimensional polyacrylamide gel electrophoresis were used to study protein expression from zooplankton collected in thirteen stations of Toulon Bay (NW Mediterranean). In this ecosystem, Little Bay showed higher trace metal concentrations (13.5-23.8 nM for Cu, 0.73-1.24 nM for Pb, 27.8-58.7 nM for Zn) than Large Bay (Cu 2.2-15.6 nM; Pb 0.19-0.78 nM; Zn 9.0-38.8 nM). Trace metals positively correlated (p < 0.05) with expression of four zooplankton proteins (MW in kDa/pI: 25.0/5.6; 48.8/4.1; 38.2/4.4; 38.3/5.8) and with biomass of Oithona nana, predominant copepod in Little Bay. Sequencing by LC-MS/MS putatively provided zooplankton identity of these proteins: they were cytoskeleton actin, except one protein that was the chaperone calreticulin. We suggest that actin and calreticulin could be regarded as zooplankton markers of metal stress and be involved in a possible tolerance of O. nana to contamination, contributing to its development in a marine perturbed ecosystem.
authors: Laboratoire Processus de Transfert et d'Echanges dans l'Environnement (EA 3819), Université du Sud Toulon - Var, BP 20132, 83 957 La Garde Cedex, France. jean@univ-tln.fr
authors: Natacha
authors: N
authors: Jean
authors: Estelle
authors: E
authors: Dumont
authors: Gael
authors: G
authors: Durrieu
authors: Thierry
authors: T
authors: Balliau
authors: Jean-Louis
authors: JL
authors: Jamet
authors: Sébastien
authors: S
authors: Personnic
authors: Cédric
authors: C
authors: Garnier
mesh_terms: D000818:Animals; D018533:Biomass; D033342:Copepoda / Q000187:drug effects; D017753:Ecosystem; D004784:Environmental Monitoring; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D008670:Metals / Q000032:analysis / Q000633:toxicity*; D012623:Seawater / Q000032:analysis; D053719:Tandem Mass Spectrometry; D014131:Trace Elements / Q000032:analysis / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D015048:Zooplankton / Q000378:metabolism*
chemical_list: D008670:Metals; D014131:Trace Elements; D014874:Water Pollutants, Chemical