title: [Expression of TEKT4 protein decreases in the ejaculated spermatozoa of idiopathic asthenozoospermic men].
abstract: OBJECTIVE
  To investigate the role of the TEKT4 protein in the pathogenesis of idiopathic asthenozoospermia.
  
  
  METHODS
  We separated and purified the ejaculated sperm from idiopathic asthenozoospermia patients and normozoospermic men by Percoll discontinuous density gradients, and detected the distribution and the expressions of TEKT4 mRNA and TEKT4 protein by RT-PCR and Western blot.
  
  
  RESULTS
  RT-PCR revealed that the expression of TEKT4 mRNA was significantly lower in the sperm of the idiopathic asthenozoospermia patients than in those of the normozoospermic men (0.59 +/- 0.13 vs 0.75 +/- 0.15, t = 4.325, P < 0.05), and Western blot confirmed the results of RT-PCR (0.48 +/- 0.14 vs 0.69 +/- 0.13, t = 5.939, P < 0.05).
  
  
  CONCLUSIONS
  The expression of TEKT4 is significantly decreased in the ejaculated sperm of idiopathic asthenozoospermia patients, which might be one of the causes of idiopathic asthenozoospermia.
authors: Center of Reproductive Medicine, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors: Wen-Bin
authors: WB
authors: Wu
authors: Yu-Shan
authors: YS
authors: Li
authors: Xiao-Fei
authors: XF
authors: Ji
authors: Quan-Xian
authors: QX
authors: Wang
authors: Xue-Min
authors: XM
authors: Gao
authors: Xian-Feng
authors: XF
authors: Yang
authors: Zhou-Hui
authors: ZH
authors: Pan
authors: Xiao-Xia
authors: XX
authors: Feng
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D003598:Cytoskeletal Proteins; D012333:RNA, Messenger