title: The presence of anti-LPS antibodies and human serum activity against Proteus mirabilis S/R forms in correlation with TLR4 (Thr399Ile) gene polymorphism in rheumatoid arthritis.
abstract: OBJECTIVE
  Proteus mirabilis strains are human pathogens responsible for urinary tract infections, which may also be involved in rheumatoid arthritis (RA).
  
  
  METHODS
  We determined whether the binding site of anti-LPS antibodies on the O-polysaccharide part of P. mirabilis LPS correlates with the level of TLR4 (Thr399Ile) gene polymorphism in the sera of RA patients. We investigated the deposition of C3d and C5b complement components on the P. mirabilis LPS. The ELISA method used in this study was optimized with LAL test and laser interferometry.
  
  
  RESULTS
  Depending on LPS P. mirabilis used in these studies, the amount of antibodies in RA patients sera varied. We did not observe a correlation between anti-LPS antibodies binding and the level of TLR4 (Thr399Ile) gene polymorphism. We found that the lower complement components deposition by O49 in contrast to O9 LPS correlates with its reduced sensitivities to human complement-mediated killing.
  
  
  CONCLUSIONS
  The immunological response against P. mirabilis LPS might play a role in rheumatoid arthritis.
authors: Institute of Biology, Department of Microbiology, Jan Kochanowski University, 25-406, Kielce, Poland. arabski@ujk.edu.pl
authors: Michal
authors: M
authors: Arabski
authors: Rafal
authors: R
authors: Fudala
authors: Anna
authors: A
authors: Koza
authors: Slawomir
authors: S
authors: Wasik
authors: Bozena
authors: B
authors: Futoma-Koloch
authors: Gabriela
authors: G
authors: Bugla-Ploskonska
authors: Wieslaw
authors: W
authors: Kaca
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution; D054458:Amplified Fragment Length Polymorphism Analysis; D000907:Antibodies, Bacterial / Q000097:blood* / Q000737:chemistry; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007368:Interferometry; D008027:Light; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011485:Protein Binding; D011513:Proteus mirabilis / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000097:blood / Q000235:genetics*
chemical_list: D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4