title: Line-scanning detection instrument for photonic crystal enhanced fluorescence.
abstract: A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a one-dimensional photonic crystal (PC) for excitation of surface-bound fluorophores. The excitation laser beam is shaped into an 8 μm × 1 mm line that is focused along the direction of the PC grating, while remaining collimated perpendicular to the grating. Such a beam configuration offers high excitation power density while simultaneously providing high resonant coupling efficiency from the laser to the PC surface. Using a panel of 21 immunofluorescence assays on the PC surface in a microarray format, the approach achieves an enhancement factor as high as 90-fold between on-resonance and off-resonance illumination. The instrument provides a capability for sensitive and inexpensive analysis of cancer biomarkers in clinical applications.
authors: Department of Electrical and Computer Engineering, University of Illinois at Urbana–Champaign, Urbana, Illinois, USA.
authors: Vikram
authors: V
authors: Chaudhery
authors: Meng
authors: M
authors: Lu
authors: Cheng-Sheng
authors: CS
authors: Huang
authors: James
authors: J
authors: Polans
authors: Ruimin
authors: R
authors: Tan
authors: Richard C
authors: RC
authors: Zangar
authors: Brian T
authors: BT
authors: Cunningham
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D049329:Nanostructures; D017785:Photons; D040081:Protein Array Analysis; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013499:Surface Properties
chemical_list: D014408:Biomarkers, Tumor