title: Dietary genistein enhances phosphorylation of regulatory myosin light chain in the myocardium of ovariectomized mice.
abstract: There is evidence that isoflavones, such as genistein, can directly or indirectly improve lipid profile and lower blood pressure and hence exert cardiovascular protection. It is further believed, that genistein attenuates vascular contraction and thus vascular tone and blood pressure through altering the phosphorylation of the regulatory myosin light chain (MLC) probably via the myosin light chain kinase (MLCK) or the RhoA pathway. However, the direct role of genistein in the myocardium is poorly reviewed. In this study, we investigated the impact of genistein on the cardiac proteome in ovariectomized female mice using a 2DE-MS approach. Dietary genistein intake considerably changed the abundance of several cytoskeletal and contractile proteins and enhanced the phosphorylation of MLC. The MLC phosphorylation was mediated through increased abundance of MLCK and inhibition of myosin light chain phosphatase latest known to be inversely regulated by RhoA. Contrary to others, in our model genistein did neither inhibit the cardiac MLCK, nor the cardiac RhoA pathway in vivo.
authors: Center for Cardiovascular Research, Institute of Pharmacology, Charité - Universitätsmedizin Berlin, Berlin, Germany. karima.schwab@charite.de
authors: Karima
authors: K
authors: Schwab
authors: Robert
authors: R
authors: Stein
authors: Christian
authors: C
authors: Scheler
authors: Franz
authors: F
authors: Theuring
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004032:Diet; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D019833:Genistein / Q000008:administration & dosage*; D006321:Heart / Q000187:drug effects*; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism; D009206:Myocardium / Q000737:chemistry* / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D043263:Myosin-Light-Chain Phosphatase / Q000378:metabolism; D010052:Ovariectomy; D010766:Phosphorylation / Q000187:drug effects*; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014335:Tropomyosin / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism; D020742:rhoA GTP-Binding Protein
chemical_list: D008840:Microfilament Proteins; D018994:Myosin Light Chains; D011506:Proteins; D020543:Proteome; D011960:Receptors, Estrogen; C490774:Tpm1 protein, mouse; D014335:Tropomyosin; C071058:moesin; D019833:Genistein; D009219:Myosin-Light-Chain Kinase; D043263:Myosin-Light-Chain Phosphatase; C526380:RhoA protein, mouse; D020741:rho GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein