title: Kinetics of nitrate and perchlorate removal and biofilm stratification in an ion exchange membrane bioreactor.
abstract: The biological degradation of nitrate and perchlorate was investigated in an ion exchange membrane bioreactor (IEMB) using a mixed anoxic microbial culture and ethanol as the carbon source. In this process, a membrane-supported biofilm reduces nitrate and perchlorate delivered through an anion exchange membrane from a polluted water stream, containing 60 mg/L of NO₃⁻ and 100 μg/L of ClO₄⁻. Under ammonia limiting conditions, the perchlorate reduction rate decreased by 10%, whereas the nitrate reduction rate was unaffected. Though nitrate and perchlorate accumulated in the bioreactor, their concentrations in the treated water (2.8 ± 0.5 mg/L of NO₃⁻ and 7.0 ± 0.8 μg/L of ClO₄⁻, respectively) were always below the drinking water regulatory levels, due to Donnan dialysis control of the ionic transport in the system. Kinetic parameters determined for the mixed microbial culture in suspension showed that the nitrate reduction rate was 35 times higher than the maximum perchlorate reduction rate. It was found that perchlorate reduction was inhibited by nitrate, since after nitrate depletion perchlorate reduction rate increased by 77%. The biofilm developed in the IEMB was cryosectioned and the microbial population was analyzed by fluorescence in situ hybridization (FISH). The results obtained seem to indicate that the kinetic advantage of nitrate reduction favored accumulation of denitrifiers near the membrane, whereas per(chlorate) reducing bacteria were mainly positioned at the biofilm outer surface, contacting the biomedium. As a consequence of the biofilm stratification, the reduction of perchlorate and nitrate occur sequentially in space allowing for the removal of both ions in the IEMB.
authors: REQUIMTE/CQFB, Department of Chemistry, FCT, Universidade Nova de Lisboa, Campus de Caprarica, P-2829-516 Caparica, Portugal.
authors: Ana R
authors: AR
authors: Ricardo
authors: Gilda
authors: G
authors: Carvalho
authors: Svetlozar
authors: S
authors: Velizarov
authors: João G
authors: JG
authors: Crespo
authors: Maria A M
authors: MA
authors: Reis
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental; D018441:Biofilms; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D058440:Denitrification; D017404:In Situ Hybridization, Fluorescence; D007474:Ion Exchange; D007700:Kinetics; D008567:Membranes, Artificial; D059013:Microbial Consortia; D009566:Nitrates / Q000302:isolation & purification*; D010472:Perchlorates / Q000302:isolation & purification*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D018508:Water Purification / Q000295:instrumentation* / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D009566:Nitrates; D010472:Perchlorates; D014874:Water Pollutants, Chemical; C494474:perchlorate