title: In vitro and in vivo activity of gallic acid and Toona sinensis leaf extracts against HL-60 human premyelocytic leukemia.
abstract: Toona sinensis is one of the most popular vegetarian cuisines in Taiwan and it has been shown to induce apoptosis in cultured human premyelocytic leukemia (HL-60) cells. In the present study, we examined the effects of T. sinensis leaf extracts (TS extracts) on tumor regression using in vitro cell culture and an in vivo athymic nude mice model. We found that TS extracts (10-75 μg/mL) arrested HL-60 cells at the G1-S transition phase through the reductions of Cyclin D1, CDK4, Cyclin E, CDK2, and Cyclin A, and induction of CDK inhibitor p27KIP levels. Furthermore, VEGF expression and release was significantly inhibited by TS extracts. Notably, TS extracts treatment was effective in terms of delaying tumor incidence in the nude mice inoculated with HL-60 cells as well as reducing the tumor burden. Histological analysis confirmed that TS extracts significantly modulated tumor progression in xenograft tumor. Furthermore, a similar pattern of results were observed from gallic acid (5 and 10 μg/mL), a major compound in TS, caused G1 arrest through regulations of cell-cycle regulatory proteins. Our data suggest that T. sinensis exerts antiproliferative effects on HL-60 cells in vitro and in vivo due mainly to the presence of gallic acid.
authors: Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan.
authors: Pei-Jane
authors: PJ
authors: Huang
authors: You-Cheng
authors: YC
authors: Hseu
authors: Meng-Shiou
authors: MS
authors: Lee
authors: K J
authors: KJ
authors: Senthil Kumar
authors: Chi-Rei
authors: CR
authors: Wu
authors: Li-Sung
authors: LS
authors: Hsu
authors: Jiunn-Wang
authors: JW
authors: Liao
authors: I-Shiung
authors: IS
authors: Cheng
authors: Ya-Ting
authors: YT
authors: Kuo
authors: Shi-Ying
authors: SY
authors: Huang
authors: Hsin-Ling
authors: HL
authors: Yang
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005707:Gallic Acid / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D008297:Male; D029582:Meliaceae / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000188:drug therapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A; D005707:Gallic Acid