title: 25-Hydroxyvitamin D depletion does not exacerbate MPTP-induced dopamine neuron damage in mice.
abstract: Recent clinical evidence supports a link between 25-hydroxyvitamin D insufficiency (serum 25-hydroxyvitamin D [25(OH)D] levels <30 ng/mL) and Parkinson's disease. To investigate the effect of 25(OH)D depletion on neuronal susceptibility to toxic insult, we induced a state of 25(OH)D deficiency in mice and then challenged them with the dopaminergic neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). We found there was no significant difference between control and 25(OH)D-deficient animals in striatal dopamine levels or dopamine transporter and tyrosine hydroxylase expression after lesioning with MPTP. Additionally, we found no difference in tyrosine hydroxylase expression in the substantia nigra pars compacta. Our data suggest that reducing 25(OH)D serum levels in mice has no effect on the vulnerability of nigral dopaminergic neurons in vivo in this model system of parkinsonism.
authors: Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, Georgia, USA.
authors: E Danielle
authors: ED
authors: Dean
authors: Lydia M
authors: LM
authors: Mexas
authors: Natalie L
authors: NL
authors: Cápiro
authors: Jeanne E
authors: JE
authors: McKeon
authors: Mahlon R
authors: MR
authors: DeLong
authors: Kurt D
authors: KD
authors: Pennell
authors: Jonathan A
authors: JA
authors: Doorn
authors: Vin
authors: V
authors: Tangpricha
authors: Gary W
authors: GW
authors: Miller
authors: Marian L
authors: ML
authors: Evatt
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism* / Q000473:pathology; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000378:metabolism* / Q000473:pathology; D020267:MPTP Poisoning / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009498:Neurotoxins / Q000009:adverse effects* / Q000494:pharmacology; D013378:Substantia Nigra / Q000378:metabolism* / Q000473:pathology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D009498:Neurotoxins; D014807:Vitamin D; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; C104450:25-hydroxyvitamin D; D014446:Tyrosine 3-Monooxygenase