title: Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein             expression in rat hippocampus.
abstract: A growing number of studies have shown that commonly used anesthetic agents             may cause neurohistopathological changes and persistent behavioral impairments             in the developing brain. The effects of sevoﬂurane, a widely used substance in             pediatric anesthesia, on the developing brain have not been thoroughly analyzed             thus far. In this study, Sprague-Dawley rats at postnatal day (P)7 were exposed             to 2.3% sevoflurane for 6 h continuously. Six hours after exposure, hematoxylin             and eosin (H&E) staining was used to determine the morphological changes in             the hippocampus. The expression levels of neuronal nitric oxide synthase (nNOS)             and caspase-3 in the hippocampus of the pups were determined by immunofluorescence.             A single 6-h sevoflurane exposure at P7 caused morphological changes in the hippocampus.             Sevoflurane also significantly increased the expression of cleaved caspase-3 and             reduced the expression of the nNOS protein. These results indicate that neonatal             exposure to sevoflurane causes neurohistopathological changes, apoptosis and decreases             nNOS protein levels in the rat hippocampus.
authors: Department of Anesthesiology, First Affiliated Hospital of Sun Yat-sen University, and Department of Anatomy, Zhong Shan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, PR China.
authors: Xue
authors: X
authors: Zhou
authors: Fa-Huan
authors: FH
authors: Song
authors: Wen
authors: W
authors: He
authors: Xiao-Yu
authors: XY
authors: Yang
authors: Zhi-Bin
authors: ZB
authors: Zhou
authors: Xia
authors: X
authors: Feng
authors: Li-Hua
authors: LH
authors: Zhou
mesh_terms: D018685:Anesthetics, Inhalation / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008738:Methyl Ethers / Q000633:toxicity*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane; D052248:Nitric Oxide Synthase Type I; D053148:Caspase 3