title: Collagen scaffolds with in situ-grown calcium phosphate for osteogenic differentiation of Wharton's jelly and menstrual blood stem cells.
abstract: The aim of this research was to investigate the osteogenic differentiation potential of non-invasively obtained human stem cells on collagen nanocomposite scaffolds with in situ-grown calcium phosphate crystals. The foams had 70% porosity and pore sizes varying in the range 50-200 µm. The elastic modulus and compressive strength of the calcium phosphate containing collagen scaffolds were determined to be 234.5 kPa and 127.1 kPa, respectively, prior to in vitro studies. Mesenchymal stem cells (MSCs) obtained from Wharton's jelly and menstrual blood were seeded on the collagen scaffolds and proliferation and osteogenic differentiation capacities of these cells from two different sources were compared. The cells on the composite scaffold showed the highest alkaline phosphatase activity compared to the controls, cells on tissue culture polystyrene and cells on collagen scaffolds without in situ-formed calcium phosphate. MSCs isolated from both Wharton's jelly and menstrual blood showed a significant level of osteogenic activity, but those from Wharton's jelly performed better. In this study it was shown that collagen nanocomposite scaffolds seeded with cells obtained non-invasively from human tissues could represent a potential construct to be used in bone tissue engineering.
authors: Department of Biotechnology, METU, Ankara, Turkey; METU Centre of Excellence in Biomaterials and Tissue Engineering, BIOMATEN, Ankara, Turkey.
authors: Ozge
authors: O
authors: Karadas
authors: Deniz
authors: D
authors: Yucel
authors: Halime
authors: H
authors: Kenar
authors: Gamze
authors: G
authors: Torun Kose
authors: Vasif
authors: V
authors: Hasirci
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001773:Blood Cells / Q000166:cytology; D002118:Calcium / Q000032:analysis; D002130:Calcium Phosphates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology* / Q000648:ultrastructure; D019245:Compressive Strength / Q000187:drug effects; D003460:Crystallization; D005260:Female; D006801:Humans; D008422:Materials Testing; D008598:Menstruation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D010012:Osteogenesis / Q000187:drug effects*; D010710:Phosphates / Q000032:analysis; D016062:Porosity; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds / Q000737:chemistry*; D059631:Wharton Jelly / Q000166:cytology*
chemical_list: D002130:Calcium Phosphates; D010710:Phosphates; D003094:Collagen; C020243:calcium phosphate; D000469:Alkaline Phosphatase; D002118:Calcium