title: Three-dimensional second-harmonic generation imaging of fibrillar collagen in biological tissues.
abstract: Multiphoton-induced second-harmonic generation (SHG) has developed into a very powerful approach for in depth visualization of some biological structures with high specificity. In this unit, we describe the basic principles of three-dimensional SHG microscopy. In addition, we illustrate how SHG imaging can be utilized to assess collagen fibrils in biological tissues. Some technical considerations are also addressed.
authors: Department of Medical Sciences, Amgen, Thousand Oaks, California, USA.
authors: Jiansong
authors: J
authors: Xie
authors: John
authors: J
authors: Ferbas
authors: Gloria
authors: G
authors: Juan
mesh_terms: D000818:Animals; D002356:Cartilage / Q000033:anatomy & histology*; D016616:Cryoultramicrotomy; D024022:Fibrillar Collagens; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton
chemical_list: D024022:Fibrillar Collagens