title: Using SecM arrest sequence as a tool to isolate ribosome bound polypeptides.
abstract: Extensive research has provided ample evidences suggesting that protein folding in the cell is a co-translational process. However, the exact pathway that polypeptide chain follows during co-translational folding to achieve its functional form is still an enigma. In order to understand this process and to determine the exact conformation of the co-translational folding intermediates, it is essential to develop techniques that allow the isolation of RNCs carrying nascent chains of predetermined sizes to allow their further structural analysis. SecM (secretion monitor) is a 170 amino acid E. coli protein that regulates expression of the downstream SecA (secretion driving) ATPase in the secM-secA operon. Nakatogawa and Ito originally found that a 17 amino acid long sequence (150-FSTPVWISQAQGIRAGP-166) in the C-terminal region of the SecM protein is sufficient and necessary to cause stalling of SecM elongation at Gly165, thereby producing peptidyl-glycyl-tRNA stably bound to the ribosomal P-site. More importantly, it was found that this 17 amino acid long sequence can be fused to the C-terminus of virtually any full-length and/or truncated protein thus allowing the production of RNCs carrying nascent chains of predetermined sizes. Thus, when fused or inserted into the target protein, SecM stalling sequence produces arrest of the polypeptide chain elongation and generates stable RNCs both in vivo in E. coli cells and in vitro in a cell-free system. Sucrose gradient centrifugation is further utilized to isolate RNCs. The isolated RNCs can be used to analyze structural and functional features of the co-translational folding intermediates. Recently, this technique has been successfully used to gain insights into the structure of several ribosome bound nascent chains. Here we describe the isolation of bovine Gamma-B Crystallin RNCs fused to SecM and generated in an in vitro translation system.
authors: Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University, USA.
authors: Sujata S
authors: SS
authors: Jha
authors: Anton A
authors: AA
authors: Komar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010441:Peptide Chain Elongation, Translational; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics; D038222:gamma-Crystallins / Q000737:chemistry* / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; C497347:SecM protein, E coli; D014157:Transcription Factors; C107738:crystallin, gammaB; D038222:gamma-Crystallins