title: Involvement of hepatitis B virus X gene (HBx) integration in hepatocarcinogenesis via a recombination of HBx/Alu core sequence/subtelomeric DNA.
abstract: The significance of hepatitis B virus (HBV) DNA-based integration in hepatocarcinogenesis is poorly understood. In the present study, we investigated whether the integration of HBV X gene (HBx) is involved in the event. Our finding showed that the integration of HBx fragment (316-462 bp/262-462 bp) was able to transform human immortalized normal liver LO2 cells using a cell model of HBx-integration. We identified that the recombination, HBx/Alu core sequence/subtelomeric DNA, was required for the transformation, which could be detected in 5 out of 44 clinical HBx-positive hepatocellular carcinoma tissues. Thus, we conclude that HBx integration is involved in the hepatocarcinogenesis.
authors: Department of Cancer Research, Key Laboratory of Molecular Microbiology and Technology of Ministry of Education, College of Life Sciences, Nankai University, PR China.
authors: Xuan
authors: X
authors: Zhang
authors: Xiaona
authors: X
authors: You
authors: Nan
authors: N
authors: Li
authors: Weiying
authors: W
authors: Zhang
authors: Sarantis
authors: S
authors: Gagos
authors: Qi
authors: Q
authors: Wang
authors: Aggelos
authors: A
authors: Banos
authors: Na
authors: N
authors: Cai
authors: Huakun
authors: H
authors: Zhang
authors: Hang
authors: H
authors: Zhang
authors: Xuezhi
authors: X
authors: Zhang
authors: Changliang
authors: C
authors: Shan
authors: Liyan
authors: L
authors: Qiu
authors: Shuai
authors: S
authors: Zhang
authors: Na
authors: N
authors: Lv
authors: Minshan
authors: M
authors: Chen
authors: Yumei
authors: Y
authors: Du
authors: Jianchuan
authors: J
authors: Xia
authors: Lihong
authors: L
authors: Ye
authors: Xiaodong
authors: X
authors: Zhang
mesh_terms: D020087:Alu Elements; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000235:genetics / Q000821:virology; D002461:Cell Line, Transformed; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D008113:Liver Neoplasms / Q000209:etiology* / Q000235:genetics / Q000821:virology; D008954:Models, Biological; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D016615:Telomere / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins; D016662:Virus Integration / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D004279:DNA, Viral; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein