title: In vitro effect of quercetin on human gastric carcinoma: targeting cancer cells death and MDR.
abstract: The benefits of plant polyphenols as chemotherapeutic agents are of great interest due to their possible anti-cancerogenic activities. Results available up to now suggest that flavonoid quercetin induces lethal effect in many types of tumours and may sensitize resistant cells to drugs. The aim of our study was to examine the effect of quercetin on human gastric carcinoma cells and to determine mode of its action. Parental EPG85-257P cell line and its daunorubicin-resistant variant EPG85-257RDB were used as cell models. Our data revealed that quercetin exerted antiproliferative impact on studied cells (with IC(50) value of 12 μM after 72 h), mainly through induction of apoptosis. In sensitive cells cytostatic drug and flavonoid had synergistic effects, in EPG85-257RDB cells quercetin acted as a chemosensitizer. Its impact on resistance mechanism involved decrease of P-glycoprotein expression, inhibition of drug transport and downregulation of ABCB1 gene expression. The results demonstrate that quercetin may be considered as a prospective drug to overcome classical resistance in gastric cancer cells.
authors: Department of Histology and Embryology, Medical University, Chalubinskiego 6a, 50-368 Wroclaw, Poland. sylwia.borska@am.wroc.pl
authors: Sylwia
authors: S
authors: Borska
authors: Magdalena
authors: M
authors: Chmielewska
authors: Teresa
authors: T
authors: Wysocka
authors: Malgorzata
authors: M
authors: Drag-Zalesinska
authors: Maciej
authors: M
authors: Zabel
authors: Piotr
authors: P
authors: Dziegiel
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000502:physiology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D003630:Daunorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D006801:Humans; D066298:In Vitro Techniques; D011794:Quercetin / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D011794:Quercetin; D003630:Daunorubicin