title: [The Trp64Arg polymorphism of beta3-adrenoreceptor gene study in persons with overweight and obesity].
abstract: The development of obesity is determined by lifestyle and genetic mechanisms. In particular, the polymorphisms in the adrenergic receptor genes (ADRB) have been extensively studied for association with obesity-related phenotypes. ADRB3 is an obvious candidate gene given its involvement in the regulation of lipolysis and thermogenesis. ADRB3 Trp64Arg polymorphism, a missense mutation in the first transmembrane domain of the R3-adrenergic receptor is associated with visceral obesity and insulin resistance in the Pima Indian, French, and Finnish populations. The recent meta-analysis that combined data of 6582 individuals from Japanese populations showed significant association the Arg64 allele with increased BMI. There are tested the polymorphisms in the beta3-Adrenoreceptor (ADRB3) gene in associated with body mass index (BMI), fat mass and biochemical parameters.We have been examined 91 persons from Moscow region with BMI >25 kg/m2. The Trp64Arg polymorphism of ADRB3 genes were genotyped with the use of an allelic discrimination assay. The TaqMan-based real-time PCR method was applied. There have been estimated of anthropometric and biochemicalparameters. The frequencies of the Trp64Trp and Trp64Arggenotypes of ADRB3 gene were 82% and 12%, respectively, the frequencies of mutant allele was 6%. Trp64Arg genotypes of ADRB3 compared to Trp64Trp genotypes had significantly higher body fat percentage (respectively 48,6 +/- 0,96% and 43,8 +/- 1,72%, p<0,05), serum glucose (6,51 +/- 0,18 mmol/l and 5,67 +/- 0,09 mmol/l, p<0,01) and uric acid concentrations (0,46 +/- 0,02 mmol/l and 0,38 +/- 0,01 mmol/l, p<0,05). The test of the ADRB3 gene polymorphisms can be used for the personalization of diet in persons with obesity.
authors: A K
authors: AK
authors: Baturin
authors: A V
authors: AV
authors: Pogozheva
authors: E Iu
authors: EIu
authors: Sorokina
authors: O N
authors: ON
authors: Makurina
authors: V A
authors: VA
authors: Tutel'ian
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001120:Arginine / Q000235:genetics; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D001835:Body Weight / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009031:Moscow; D009154:Mutation; D009765:Obesity / Q000097:blood / Q000178:diet therapy / Q000235:genetics*; D050177:Overweight / Q000097:blood / Q000178:diet therapy / Q000235:genetics; D011110:Polymorphism, Genetic; D060888:Real-Time Polymerase Chain Reaction; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics*; D012720:Severity of Illness Index; D014364:Tryptophan / Q000235:genetics; D014527:Uric Acid / Q000097:blood; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D022702:Receptors, Adrenergic, beta-3; D014527:Uric Acid; D014364:Tryptophan; D001120:Arginine