title: A pro-cathepsin L mutant is a luminal substrate for endoplasmic-reticulum-associated degradation in C. elegans.
abstract: Endoplasmic-reticulum associated degradation (ERAD) is a major cellular misfolded protein disposal pathway that is well conserved from yeast to mammals. In yeast, a mutant of carboxypeptidase Y (CPY*) was found to be a luminal ER substrate and has served as a useful marker to help identify modifiers of the ERAD pathway. Due to its ease of genetic manipulation and the ability to conduct a genome wide screen for modifiers of molecular pathways, C. elegans has become one of the preferred metazoans for studying cell biological processes, such as ERAD. However, a marker of ERAD activity comparable to CPY* has not been developed for this model system. We describe a mutant of pro-cathepsin L fused to YFP that no longer targets to the lysosome, but is efficiently eliminated by the ERAD pathway. Using this mutant pro-cathepsin L, we found that components of the mammalian ERAD system that participate in the degradation of ER luminal substrates were conserved in C. elegans. This transgenic line will facilitate high-throughput genetic or pharmacological screens for ERAD modifiers using widefield epifluorescence microscopy.
authors: Department of Pediatrics, Cell Biology and Physiology, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC, Pittsburgh, Pennsylvania, USA.
authors: Mark T
authors: MT
authors: Miedel
authors: Nathan J
authors: NJ
authors: Graf
authors: Kate E
authors: KE
authors: Stephen
authors: Olivia S
authors: OS
authors: Long
authors: Stephen C
authors: SC
authors: Pak
authors: David H
authors: DH
authors: Perlmutter
authors: Gary A
authors: GA
authors: Silverman
authors: Cliff J
authors: CJ
authors: Luke
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D043402:Cathepsin A / Q000235:genetics / Q000378:metabolism*; D056668:Cathepsin L / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000201:enzymology* / Q000235:genetics; D060746:Endoplasmic Reticulum-Associated Degradation / Q000502:physiology*; D020125:Mutation, Missense; D017510:Protein Folding
chemical_list: D029742:Caenorhabditis elegans Proteins; D043402:Cathepsin A; C466023:CPL-1 protein, C elegans; D056668:Cathepsin L