title: Isolation and characterization of a bacteriophage F20 virulent to Enterobacter aerogenes.
abstract: An aquatic phage, designated F20, was characterized and its physico-chemical characteristics studied. F20 was specifically virulent to only two strains of Enterobacter aerogenes (ATCC 13048 and the multi-drug-resistant strain K113) among other species tested (n = 15). It was classified in the family Siphoviridae of T1-like viruses and contained a linear dsDNA genome estimated to be 51.5 kbp enclosed by an isometric capsid of 50±2 nm in diameter and a tail of 150±3 nm in length. F20 was able to survive in a broad pH range between 4 and 11, showed potential for future animal trials using oral solution and resisted chloroform and ethanol. It exhibited remarkable stability between room temperature and 70 °C for up to 150 min, and even up to 6 months at room temperature. Knowledge of this phage belonging to the widespread T1-like viruses might be helpful for adopting therapeutic strategies against E. aerogenes.
authors: Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk 712-714, Republic of Korea.
authors: Charitra Kumar
authors: CK
authors: Mishra
authors: Department of Microbiology, Pukyong National University, 599-1, Daeyeon 3-Dong, Nam-Gu, Busan, 608-737, Republic of Korea.
authors: Tae Jin
authors: TJ
authors: Choi
authors: Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk 712-714, Republic of Korea.
authors: Sun Chul
authors: SC
authors: Kang
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D021902:Enterobacter aerogenes / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D017906:Siphoviridae / Q000235:genetics / Q000302:isolation & purification; D013696:Temperature
chemical_list: D004279:DNA, Viral; D004247:DNA