title: In-cell NMR in mammalian cells: part 3.
abstract: Irrespective of how isotope-labeled proteins are delivered into mammalian cells, laboratory routines are needed to assess the quality of the resulting in-cell NMR samples. These include methods to evaluate overall cell viability, protein transduction efficiency, intracellular protein concentration, localization, and stability. In addition, quality control experiments to assess protein leakage from manipulated cells are of particular importance for in-cell NMR experiments. The purpose of this chapter is to outline qualitative and quantitative methods to determine general biological properties of in-cell NMR samples in order to ensure the highest possible standards for in-cell NMR studies.
authors: Department of NMR-assisted Structural Biology, Leibniz Institute of Molecular Pharmacology (FMP Berlin), Berlin, Germany.
authors: Beata
authors: B
authors: Bekei
authors: Honor May
authors: HM
authors: Rose
authors: Michaela
authors: M
authors: Herzig
authors: Heike
authors: H
authors: Stephanowitz
authors: Eberhard
authors: E
authors: Krause
authors: Philipp
authors: P
authors: Selenko
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D013194:Staining and Labeling; D053719:Tandem Mass Spectrometry; D014343:Trypan Blue / Q000737:chemistry; D051844:alpha-Synuclein / Q000737:chemistry*
chemical_list: D051844:alpha-Synuclein; D014343:Trypan Blue