title: Cytochrome P450 system as potential biomarkers of certain toxicants: comparison between plant and animal models.
abstract: In present study, we measured the activities of some selected cytochrome P450 isozymes like ethoxyresorufin-o-deethylase (EROD), pentoxyresorufin-o-deethylase (PROD), and N-nitrosodimethylamine demethylase (NDMA-d) treating experimental rats with different environmental toxicants, namely trichloroethylene (TCE), heavy metal mixture, and ethyl alcohol individually or in combination and by exposing the Allium cepa bulbs to increasing concentration of TCE for 48 h. In animal system, NDMA-d activity displayed a remarkable amplification by 4.2-fold in the liver of alcohol ingested rats compared with control animals. TCE intake also resulted in a marked increase of around 3.8-fold and 1.2-fold in rats' NDMA-d activity in the liver and kidney, respectively. In onion bulbs, an amazing rise in the activity of test enzymes was recorded in a dose-dependent manner. Among the said enzymes, PROD showed maximum increase up to the extent of 22-fold in comparison with control at 20 ppm of TCE exposure, whereas NDMA-d and EROD showed 11- and 9-fold increase in enzymatic activity, respectively, compared with the control. Based on this study, we conclude and advocate that all the selected isozymes of CYP450 system, viz. PROD, NDMA-d, and EROD can act as potent biomarkers in plant system for assessing the TCE pollution.
authors: King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia. shamstabrez1@gmail.com
authors: Shams
authors: S
authors: Tabrez
authors: Masood
authors: M
authors: Ahmad
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D015386:Hazardous Substances / Q000633:toxicity*; D019216:Metals, Heavy / Q000633:toxicity; D008954:Models, Biological; D019697:Onions / Q000378:metabolism; D010944:Plants / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D014241:Trichloroethylene / Q000633:toxicity
chemical_list: D015415:Biomarkers; D015386:Hazardous Substances; D019216:Metals, Heavy; D014241:Trichloroethylene; D003577:Cytochrome P-450 Enzyme System; D019392:Cytochrome P-450 CYP2E1; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1