title: Development of an electrochemical DNA biosensor with the DNA immobilization based on in situ generation of dithiocarbamate ligands.
abstract: In this article, a simple and effective strategy for DNA immobilization on gold electrode surface is developed. The amine-modified oligonucleotide was firstly reacted with CS(2) and then in situ generated dithiocarbamate group functionalized probe DNA (DTC-DNA) was directly attached onto the gold surface by bidentate anchoring points. The DNA biosensor fabrication process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy with the use of Fe(CN)(6)(3-/4-) as a redox indicator. The hybridization of DTC-DNA with complementary target DNA could be well distinguished with the use of Co(phen)(3)(3+) as an electrochemical indicator. The fabricated electrochemical DNA biosensor could achieve a detection limit of about 0.1nM toward complementary target DNA. Also, the current strategy is readily operated with less time consumed and lower cost compared with those commonly used strategies.
authors: Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors: Li
authors: L
authors: Wang
authors: Xiaoli
authors: X
authors: Wang
authors: Xiaohong
authors: X
authors: Chen
authors: Jing
authors: J
authors: Liu
authors: Shufeng
authors: S
authors: Liu
authors: Changzhi
authors: C
authors: Zhao
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008024:Ligands; D013499:Surface Properties; D013859:Thiocarbamates / Q000737:chemistry
chemical_list: D015342:DNA Probes; D005292:Ferricyanides; D008024:Ligands; D013859:Thiocarbamates; D006046:Gold; D004247:DNA; C053439:ferric ferricyanide