title: Acetylation regulates subcellular localization of eukaryotic translation initiation factor 5A (eIF5A).
abstract: Eukaryotic translation initiation factor 5A (eIF5A) is a protein subject to hypusination, which is essential for its function. eIF5A is also acetylated, but the role of that modification is unknown. Here, we report that acetylation regulates the subcellular localization of eIF5A. We identified PCAF as the major cellular acetyltransferase of eIF5A, and HDAC6 and SIRT2 as its major deacetylases. Inhibition of the deacetylases or impaired hypusination increased acetylation of eIF5A, leading to nuclear accumulation. As eIF5A is constitutively hypusinated under physiological conditions, we suggest that reversible acetylation plays a major role in controlling the subcellular localization of eIF5A.
authors: Chemical Genetic Laboratory, RIKEN Advanced Science Institute, Wako, Saitama, Japan.
authors: Muhammad
authors: M
authors: Ishfaq
authors: Kazuhiro
authors: K
authors: Maeta
authors: Satoko
authors: S
authors: Maeda
authors: Toru
authors: T
authors: Natsume
authors: Akihiro
authors: A
authors: Ito
authors: Minoru
authors: M
authors: Yoshida
mesh_terms: D000107:Acetylation; D021581:Active Transport, Cell Nucleus; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D000073864:Histone Deacetylase 6; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D056565:Sirtuin 2 / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D050880:p300-CBP Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000097574:Eukaryotic Translation Initiation Factor 5A; D000099224:p300-CBP-Associated Factor
chemical_list: D000073864:Histone Deacetylase 6; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases; D008239:Lysine; D010448:Peptide Initiation Factors; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D056565:Sirtuin 2; D050880:p300-CBP Transcription Factors; D000097574:Eukaryotic Translation Initiation Factor 5A; D000099224:p300-CBP-Associated Factor; C100028:hypusine; C474199:SIRT2 protein, human; C433811:HDAC6 protein, human