title: Monitoring of the multiple bacteriocin production by Enterococcus faecium NKR-5-3 through a developed liquid chromatography and mass spectrometry-based quantification system.
abstract: Enterococcus faecium NKR-5-3 produces four antimicrobial peptides referred here as enterocins NKR-5-3A, B, C and D. A two-step electrospray ionization-liquid chromatography and mass spectrometry (ESI-LC/MS)-based quantification system was developed to monitor its multiple bacteriocin production profiles, which is essential in understanding the complex production regulation mechanism of strain NKR-5-3. The developed ESI-LC/MS-based quantification system can easily monitor the multiple bacteriocin production of this strain. Using the developed system, the production of enterocin NKR-5-3B was found to be not as variable as those of the other enterocins in different cultivation media. Production of enterocin NKR-5-3B was also found to have a wider optimum incubation temperature (20-30°C) than enterocins NKR-5-3A, C and D (25°C). Furthermore, at least 2 nM of the bacteriocin-like inducing peptide, enterocin NKR-5-3D, regulated the production of NKR-5-3 enterocins except enterocin NKR-5-3B. These findings taken together suggest that enterocin NKR-5-3B has an independent production regulation mechanism from the other NKR-5-3 enterocins. The developed system could effectively pin-point the production profiles of the multiple bacteriocins of E. faecium NKR-5-3 under different fermentation conditions.
authors: Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors: Rodney H
authors: RH
authors: Perez
authors: Kohei
authors: K
authors: Himeno
authors: Naoki
authors: N
authors: Ishibashi
authors: Yoshimitsu
authors: Y
authors: Masuda
authors: Takeshi
authors: T
authors: Zendo
authors: Koji
authors: K
authors: Fujita
authors: Pongtep
authors: P
authors: Wilaipun
authors: Vichien
authors: V
authors: Leelawatcharamas
authors: Jiro
authors: J
authors: Nakayama
authors: Kenji
authors: K
authors: Sonomoto
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000096:biosynthesis*; D001430:Bacteriocins / Q000032:analysis / Q000096:biosynthesis*; D002853:Chromatography, Liquid / Q000379:methods*; D016984:Enterococcus faecium / Q000378:metabolism*; D005285:Fermentation; D010455:Peptides / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010455:Peptides