title: Validation of the protoporphyrin IX-triplet state lifetime technique for mitochondrial oxygen measurements in the skin.
abstract: Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that mitochondrial PO(2) (mitoPO(2)) can be measured in the skin of a rat after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Calibration of mitoPO(2) measurements was done by comparison with simultaneous measurements of the cutaneous microvascular PO(2) This was done under three different conditions: in normal skin tissue, in nonrespiration skin tissue due to the application of cyanide, and in anoxic skin tissue after the ventilation with 100% nitrogen. The results of this study show that it is feasible to measure the mitoPO(2) after the topical application of ALA cream by means of the PpIX-triplet state lifetime technique.
authors: Department of Anesthesiology, Laboratory of Experimental Anesthesiology, Erasmus MC—University Medical Center Rotterdam, Rotterdam, The Netherlands. f.harms@erasmusmc.nl
authors: Floor A
authors: FA
authors: Harms
authors: Sander I A
authors: SI
authors: Bodmer
authors: Nicolaas J H
authors: NJ
authors: Raat
authors: Robert J
authors: RJ
authors: Stolker
authors: Egbert G
authors: EG
authors: Mik
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology; D000818:Animals; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D055620:Optical Phenomena; D010100:Oxygen / Q000378:metabolism*; D011524:Protoporphyrins / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D012867:Skin / Q000166:cytology*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX; D010100:Oxygen