title: Ischemia/reperfusion in clamped lobes facilitates liver regeneration of non-clamped lobes after selective portal vein ligation.
abstract: BACKGROUND
  Hypertrophy of non-clamped liver lobes and the atrophy of clamped lobes have been shown to be interactive. Here, a rat model of selective lobe occlusion was established to study the effect of contralateral ischemia/reperfusion (I/R) on regeneration of non-clamped lobes.
  
  
  METHODS
  Left lateral and middle liver lobes were pretreated with I/R. In the experimental (IR + PVL) group, portal veins of the left and middle lobes were ligated. A group given similar portal vein ligation but no I/R (PVL) was the positive control.
  
  
  RESULTS
  Compared with the PVL group, the IR + PVL had higher, but not remarkable, levels of serum transaminases; weights of non-clamped lobes in the IR + PVL group comparatively increased much more notably. At 24-h post-surgery, the IR + PVL group's PCNA mRNA was up-regulated compared with the PVL group. At 72-h post-surgery, PCNA protein was up-regulated significantly, while TGF-β1 was down-regulated in the IR + PVL group notably, compared with the PVL group.
  
  
  CONCLUSIONS
  The I/R pretreatment given to the clamped lobes facilitates liver regeneration of non-clamped lobes after selective portal vein ligation, which may result from down-regulated TGF-β1 expression in non-clamped lobes.
authors: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, 157 Baojian Road, Harbin, 150086, China.
authors: Jian-Hua
authors: JH
authors: Yu
authors: Wei-Guang
authors: WG
authors: Zhang
authors: Gui-Xing
authors: GX
authors: Jiang
authors: Jia-Yun
authors: JY
authors: Zhao
authors: Hui
authors: H
authors: Li
authors: Zhi-Dong
authors: ZD
authors: Wang
authors: Yun-Fu
authors: YF
authors: Cui
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D006984:Hypertrophy; D008026:Ligation; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D011169:Portal Vein / Q000601:surgery*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000473:pathology*; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D053773:Transforming Growth Factor beta1; D012313:RNA