title: Individual variability of mytimycin gene expression in mussel.
abstract: The antifungal peptide mytimycin (MytM) is synthesized by hemocytes of the Mediterranean mussel, Mytilus galloprovincialis. In addition to sequence and gene structure diversities previously reported from pooled hemocytes, the present report focused on the expression of mytm gene in individual M. galloprovincialis, before and after challenge. Within untreated mussel, MytM mRNA was observed by ISH in about 42% of circulating hemocytes, characterized by large, diffuse nucleus. Injection with Fusarium oxysporum increased such percentage, but in only some of the mussels. Similarly, MytM gene expression increased after injection in only some of the mussels, as measured by qPCR. Responders and not responders are common evidence in any given population of organisms. Nevertheless, even if the use of proper pool size selection has been practised to find out and evaluate the most common response trends, individual analyses must be regarded as optimal.
authors: Ecologie des Systèmes Marins et Côtiers-EcoSym UMR5119, Université Montpellier 2-CNRS, F-34095 Montpellier Cedex 05, France.
authors: Franck
authors: F
authors: Cantet
authors: Mylène
authors: M
authors: Toubiana
authors: Maria-Giovanna
authors: MG
authors: Parisi
authors: Molruedee
authors: M
authors: Sonthi
authors: Matteo
authors: M
authors: Cammarata
authors: Philippe
authors: P
authors: Roch
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism*; D005670:Fusarium / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006434:Hemocytes / Q000378:metabolism; D017403:In Situ Hybridization; D049878:Mytilus / Q000378:metabolism* / Q000382:microbiology*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D023181:Antimicrobial Cationic Peptides; D012333:RNA, Messenger