title: Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome.
abstract: OBJECTIVE
  To evaluate the level of ribosomal RNA (rRNA) in cumulus cells (CCs) from women with polycystic ovary syndrome (PCOS) undergoing ICSI.
  
  
  METHODS
  The study population included three healthy oocyte donors (control) and three patients with PCOS. RNA expression in CCs was assessed using quantitative real-time PCR assay to measure the pre-rRNA transcripts (5'ETS region), 18 S, 5.8 S and 28 S rRNA.
  
  
  RESULTS
  The level of 18 S rRNA is 3.9 times higher (p = 0.036) and the level of 5.8 S rRNA is 2.9 times higher (p = 0.049) in CCs of PCOS patients than in CCs of healthy women. The fold change in expression of 28 S rRNA in CCs of PCOS patients also exceeds that in the control group, but did not reach a statistical significance (p = 0.342).
  
  
  CONCLUSIONS
  Our observations support the idea that CCs of PCOS patients contain comparatively more ribosomes that CCs of healthy oocyte donors that may indicate a higher proliferation rate or up-regulated translation of protein factors in ССs of PCOS patients.
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya St, 117997 Moscow, Russia. polzikov@yahoo.co.uk
authors: Mikhail
authors: M
authors: Polzikov
authors: Sergey
authors: S
authors: Yakovenko
authors: Julia
authors: J
authors: Voznesenskaya
authors: Maria
authors: M
authors: Troshina
authors: Olga
authors: O
authors: Zatsepina
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D054885:Cumulus Cells / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S