title: Medical ozone therapy decreases postoperative uterine adhesion formation in rats.
abstract: OBJECTIVE
  Various studies have been performed to find out novel treatment strategies to prevent postoperative adhesion formation. Ozone therapy (OT) is shown to reduce inflammation in several pathological conditions. Therefore, we aimed to evaluate the efficacy of OT in a rat model of experimental uterine adhesion (EUA).
  
  
  METHODS
  Thirty female Wistar rats (200-250 g) were divided into three groups: sham, EUA and EUA+OT. EUA and EUA+OT groups were subjected to the postoperative adhesion procedure by bipolar coagulation on the uterine horns and corresponding pelvic sidewall parietal peritoneum. EUA+OT group received 0.7 mg/kg daily single dose for 3 days of ozone/oxygen mixture intraperitoneally after adhesion induction. All animals were killed on the 7th day and uterine adhesions were scored. Uterine tissues and peritoneal washing fluid were harvested for all analyses.
  
  
  RESULTS
  Uterine malondialdehyde levels in the EUA group were significantly higher compared to the other groups. However, in the EUA group, uterine superoxide dismutase and glutathione peroxidase activities were lower than in other groups. Peritoneal fluid TNF-α levels were found to be significantly different for all groups (p < 0.001). Macroscopic total adhesion score was significantly higher in the EUA group compared to the other groups (p < 0.001). But, total score in the EUA+OT group was lower than in the EUA group (p = 0.006).
  
  
  CONCLUSIONS
  Medical OT prevents postoperative uterine adhesions by modulating TNF-α levels and oxidative/antioxidative status in an experimental uterine adhesion model.
authors: Department of Physiology, Gulhane Military Medical Academy, Ankara, Turkey.
authors: Bulent
authors: B
authors: Uysal
authors: Suzi
authors: S
authors: Demirbag
authors: Yavuz
authors: Y
authors: Poyrazoglu
authors: Tuncer
authors: T
authors: Cayci
authors: Narter
authors: N
authors: Yesildaglar
authors: Ahmet
authors: A
authors: Guven
authors: Ilhami
authors: I
authors: Sürer
authors: Ahmet
authors: A
authors: Korkmaz
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism; D004195:Disease Models, Animal; D004564:Electrocoagulation; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control; D008315:Malondialdehyde / Q000378:metabolism; D010083:Oxidants, Photochemical / Q000627:therapeutic use*; D018384:Oxidative Stress; D010126:Ozone / Q000627:therapeutic use*; D010537:Peritoneum / Q000601:surgery; D011183:Postoperative Complications / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D000267:Tissue Adhesions / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014599:Uterus / Q000378:metabolism / Q000601:surgery
chemical_list: D010083:Oxidants, Photochemical; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D010126:Ozone; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase