title: Stress-induced HSP70 from Musca domestica plays a functionally significant role in the immune system.
abstract: As important molecular chaperones, members of the 70kDa heat shock protein (HSP70) family play essential roles in stress tolerance and innate immunity in organisms. The full-length complementary DNA (cDNA) of a novel inducible HSP70, named as MdHSP70, was isolated from Musca domestica. The cDNA clone consisted of 2411 bp with a 1956 bp open reading frame which encodes 651 amino acids. Using real-time quantitative polymerase chain reaction (qPCR), we investigated the transcriptional profile of the gene under heat shock, cadmium stress and in response to bacteria. Increased expression of MdHSP70 was observed in response to both heat shock and Cd stress. The expression of MdHSP70 was significantly induced by Escherichia coli or Staphylococcus aureus stimulation. Larvae were fed bacteria expressing dsRNA targeting the MdHSP70 gene. Our results showed high mortality in larvae treated with dsRNA of MdHSP70 at heat shock, Cd stress and bacterial invasion, suggesting that MdHSP70 is potentially involved in the stress and immune responses of the house fly and perhaps contributes to protection against cellular injury.
authors: College of Life Sciences, Hebei University, Baoding 071002, China.
authors: Ting
authors: T
authors: Tang
authors: Chen
authors: C
authors: Wu
authors: Jigang
authors: J
authors: Li
authors: Guodong
authors: G
authors: Ren
authors: Dawei
authors: D
authors: Huang
authors: Fengsong
authors: F
authors: Liu
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002104:Cadmium / Q000378:metabolism; D004926:Escherichia coli; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D006793:Houseflies / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D034622:RNA Interference; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus; D013312:Stress, Physiological
chemical_list: D018840:HSP70 Heat-Shock Proteins; D019476:Insect Proteins; D002104:Cadmium