title: Infrared spectral histopathology (SHP): a novel diagnostic tool for the accurate classification of lung cancer.
abstract: We report results of a study utilizing a recently developed tissue diagnostic method, based on label-free spectral techniques, for the classification of lung cancer histopathological samples from a tissue microarray. The spectral diagnostic method allows reproducible and objective diagnosis of unstained tissue sections. This is accomplished by acquiring infrared hyperspectral data sets containing thousands of spectra, each collected from tissue pixels about 6 μm on edge; these pixel spectra contain an encoded snapshot of the entire biochemical composition of the pixel area. The hyperspectral data sets are subsequently decoded by methods of multivariate analysis, which reveal changes in the biochemical composition between tissue types, and between various stages and states of disease. In this study, a detailed comparison between classical and spectral histopathology (SHP) is presented, which suggests SHP can achieve levels of diagnostic accuracy that is comparable to that of multi-panel immunohistochemistry.
authors: Laboratory for Spectral Diagnosis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA.
authors: Benjamin
authors: B
authors: Bird
authors: Milo Sbreve
authors: MS
authors: Miljković
authors: Stan
authors: S
authors: Remiszewski
authors: Ali
authors: A
authors: Akalin
authors: Mark
authors: M
authors: Kon
authors: Max
authors: M
authors: Diem
mesh_terms: D006801:Humans; D008175:Lung Neoplasms / Q000145:classification / Q000175:diagnosis*; D013055:Spectrophotometry, Infrared / Q000379:methods*