title: Proteomic analysis of liver development of lean Pekin duck (Anas platyrhynchos domestica).
abstract: The liver plays vital roles in digestion, metabolism and immune defense. To elucidate the molecular mechanism of nutrient metabolism and antioxidation of lean Pekin duck liver from hatching to slaughter, the proteome changes were investigated using 2-DE, MS, quantitative real-time PCR and bioinformatics. A total of 59 differentially expressed proteins were identified. Proteins involved in transportation were highly up-regulated in newborn ducks whereas 37 proteins associated with metabolism, defense and antioxidation were up-regulated in adult ducks. The over-expression of proteins at the last developmental stage presumably occurs to fulfill the needs of multiple functions of the liver. However, the over-expressed proteins related to transportation during the first developmental stage are involved in maintaining the high basal metabolism of newborn ducks. The functional enrichment analysis also confirmed these results. Furthermore, the protein interaction network predicted 28 proteins acting as key nodes for liver development. The validated expression between proteins and genes provides us target genes for future genetic analyses to improve the health and performance of these ducks. These significant advanced proteome data expand our knowledge on the physiology of the duck liver, thereby providing a potentially valuable foundation for molecular breeding to enhance feed efficiency and immunity and for optimizing the feeding strategy.
authors: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors: Aijuan
authors: A
authors: Zheng
authors: Guohua
authors: G
authors: Liu
authors: Yunsheng
authors: Y
authors: Zhang
authors: Shuisheng
authors: S
authors: Hou
authors: Wenhuan
authors: W
authors: Chang
authors: Shu
authors: S
authors: Zhang
authors: Huiyi
authors: H
authors: Cai
authors: Guilan
authors: G
authors: Chen
mesh_terms: D000818:Animals; D001481:Basal Metabolism / Q000502:physiology; D016000:Cluster Analysis; D004372:Ducks / Q000254:growth & development* / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D008297:Male; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D013851:Thinness / Q000378:metabolism / Q000662:veterinary; D054928:Validation Studies as Topic
chemical_list: D020543:Proteome