title: Effect of the best compatibility of components in Corni Fructus on WT1 expression in glomerular podocytes of type 2 diabetic rats with early nephropathy.
abstract: Our previous studies have shown that the best compatibility of components in Corni Fructus (PC) had a protective effect on vascular endothelial cells. In this study, the effect of PC on WT1 expression in glomerular podocytes and the mechanism of PC on early nephropathy in type 2 diabetic rats have been investigated. Type 2 diabetic rats were generated by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin (STZ), then fed aminoguanidine (100 mg/kg), glimepiride (0.4 mg/kg), low-dose (60 mg/kg) or high-dose (120 mg/kg) of PC once a day for 12 weeks. Fasting blood glucose (FBG) was examined regularly. Insulin (INS) was measured by a radioimmunoassay. Microalbuminuria (mALB) was measured by an ELISA assay. Urinary creatinine (UCr), blood urea nitrogen (BUN) and N-acetyl-β-D-glucosaminidase (NAG) were measured by colorimetric assays. Renal morphological changes were observed by optical microscopy. The expression index of Wilms tumor 1 (WT1EI) in glomeruli was examined by immunohistochemistry. The results showed that PC led to a decrease in FBG (p < 0.01), NAG (p < 0.05) and mALB (p < 0.05) and an increase in INS (p < 0.01) and WT1EI (p < 0.05) compared to the diabetic group. PC could improve renal damage and greatly reduce the renal morphology score (p < 0.05). These results suggested that PC had the protective effect on early nephropathy in type 2 diabetic rats, which was closely related to the regulation of podocytes.
authors: College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, China.
authors: Hong
authors: H
authors: Liu
authors: Huiqing
authors: H
authors: Xu
authors: Cunsi
authors: C
authors: Shen
authors: Chen
authors: C
authors: Wu
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000419:Albuminuria / Q000517:prevention & control; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D029768:Cornus / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000378:metabolism; D059305:Diet, High-Fat; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005638:Fruit; D007328:Insulin / Q000097:blood; D008297:Male; D008517:Phytotherapy; D050199:Podocytes / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D025721:WT1 Proteins / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D007328:Insulin; D025721:WT1 Proteins; D000118:Acetylglucosaminidase