title: Mineralized synthetic matrices as an instructive microenvironment for osteogenic differentiation of human mesenchymal stem cells.
abstract: The effect of substrate-mediated signals on osteogenic differentiation of hMSCs is studied using a synthetic bone-like material comprising both organic and inorganic components that supports adhesion, spreading, and proliferation of hMSCs. hMSCs undergo osteogenic differentiation even in the absence of osteogenesis-inducing supplements. They exhibit higher expressions of Runx2, BSP, and OCN compared to their matrix-rigidity-matched, non-mineralized hydrogel counterparts. The mineralized-hydrogel-assisted osteogenic differentiation of hMSCs could be attributed to their exposure to high local concentrations of calcium and phosphate ions in conjunction with chemical and topological cues arising from the hydrogel-bound calcium phosphate mineral layer.
authors: Department of Bioengineering, University of California San Diego, La Jolla, CA 92093, USA.
authors: Ameya
authors: A
authors: Phadke
authors: Yu-Ru V
authors: YR
authors: Shih
authors: Shyni
authors: S
authors: Varghese
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D006801:Humans; D020100:Hydrogels; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000179:Acrylates; D015415:Biomarkers; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D050659:Core Binding Factor Alpha 1 Subunit; D020100:Hydrogels; D058574:Integrin-Binding Sialoprotein; C493709:RUNX2 protein, human; C020243:calcium phosphate; D000469:Alkaline Phosphatase