title: Proteomic study participating the enhancement of growth and salt tolerance of bottle gourd rootstock-grafted watermelon seedlings.
abstract: An insertion grafting technique to do research on salt tolerance was applied using watermelon (Citrullus lanatus [Thunb.] Mansf. cv. Xiuli) as a scion and bottle gourd (Lagenaria siceraria Standl. cv. Chaofeng Kangshengwang) as a rootstock. Rootstock-grafting significantly relieved the inhibition of growth and photosynthesis induced by salt stress in watermelon plants. Proteomic analysis revealed 40 different expressed proteins in response to rootstock-grafting and/or salt stress. These proteins were involved in Calvin cycle, amino acids biosynthesis, carbohydrate and energy metabolism, ROS defense, hormonal biosynthesis and signal transduction. Most of these proteins were up-regulated by rootstock-grafting and/or susceptible to salt stress. The enhancement of the metabolic activities of Calvin cycle, biosynthesis of amino acids, carotenoids and peroxisomes, glycolytic pathway and tricarboxylic acid cycle will probably contribute to intensify the biomass and photosynthetic capacity in rootstock-grafted seedlings under condition without salt. The accumulation of key enzymes included in these biological processes described above seems to play an important role in the enhancement of salt tolerance of rootstock-grafted seedlings. Furthermore, leucine-rich repeat transmembrane protein kinase and phospholipase may be involved in transmitting the internal and external stimuli induced by grafting and/or salt stress.
authors: Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors: Yanjuan
authors: Y
authors: Yang
authors: Liping
authors: L
authors: Wang
authors: Jing
authors: J
authors: Tian
authors: Jing
authors: J
authors: Li
authors: Jin
authors: J
authors: Sun
authors: Lizhong
authors: L
authors: He
authors: Shirong
authors: S
authors: Guo
authors: Takafumi
authors: T
authors: Tezuka
mesh_terms: D000383:Agriculture / Q000379:methods*; D029773:Citrullus / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D019662:Cucurbitaceae / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D010740:Phospholipases / Q000378:metabolism; D010788:Photosynthesis; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots; D011494:Protein Kinases / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D055049:Salt Tolerance; D036226:Seedlings / Q000502:physiology; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology; D013312:Stress, Physiological; D015854:Up-Regulation
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012965:Sodium Chloride; D011494:Protein Kinases; D010740:Phospholipases