title: Low-molecular mass comparative proteome of four atypical enteropathogenic Escherichia coli isolates showing different adherence patterns.
abstract: Atypical enteropathogenic Escherichia coli (aEPEC) are heterogeneous in terms of serotypes, adherence patterns and the presence of non-locus of enterocyte effacement virulence factors. In this study, the low-molecular mass proteomes of four representative aEPEC, comprising three different adhesion phenotypes (localized-like, aggregative and diffuse) and one non-adherent isolate, were analyzed and compared by 2D gel electrophoresis and LC-MS/MS. By mass spectrometry, a total of 59 proteins were identified according to their annotated function, with most of them being involved in metabolism, protection, and transport; some of them still classified as hypothetical proteins. Thus, in this comparative proteomic analysis of low-molecular mass extracted proteins from different aEPEC isolates, the proteins identified are mainly involved in key metabolic pathways. Also, the majority of the hypothetical and filamentous proteins identified in the isolates studied are products of genes originally identified in the genome of enterohemorrhagic E. coli.
authors: Laboratório de Bacteriologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, SP, Brazil.
authors: Júlia M
authors: JM
authors: Nara
authors: Daniel C
authors: DC
authors: Pimenta
authors: Cecília M
authors: CM
authors: Abe
authors: Patrícia A E
authors: PA
authors: Abreu
authors: Claudia T P
authors: CT
authors: Moraes
authors: Natália C
authors: NC
authors: Freitas
authors: Waldir P
authors: WP
authors: Elias
authors: Roxane M F
authors: RM
authors: Piazza
mesh_terms: D001422:Bacterial Adhesion; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D054308:Enteropathogenic Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D020543:Proteome; D037521:Virulence Factors