title: Oncogenic potential diverge among human papillomavirus type 16 natural variants.
abstract: We compared E6/E7 protein properties of three different HPV-16 variants: AA, E-P and E-350G. Primary human foreskin keratinocytes (PHFK) were transduced with HPV-16 E6 and E7 and evaluated for proliferation and ability to grow in soft agar. E-P infected keratinocytes presented the lowest efficiency in colony formation. AA and E-350G keratinocytes attained higher capacity for in vitro transformation. We observed similar degradation of TP53 among HPV-16 variants. Furthermore, we accessed the expression profile in early (p5) and late passage (p30) transduced cells of 84 genes commonly involved in carcinogenesis. Most differences could be attributed to HPV-16 E6/E7 expression. In particular, we detected different expression of ITGA2 and CHEK2 in keratinocytes infected with AA and AA/E-350G late passage cells, respectively, and higher expression of MAP2K1 in E-350G transduced keratinocytes. Our results indicate differences among HPV-16 variants that could explain, at least in part, differences in oncogenic potential attributed to these variants.
authors: Molecular Biology Laboratory, Center of Translational Oncology, Instituto do Câncer do Estado de São Paulo-ICESP, São Paulo 01246-000, Brazil. lsichero@gmail.com
authors: Laura
authors: L
authors: Sichero
authors: João Simão
authors: JS
authors: Sobrinho
authors: Luisa Lina
authors: LL
authors: Villa
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling; D014644:Genetic Variation; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D006801:Humans; D015603:Keratinocytes / Q000821:virology; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins / Q000235:genetics / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000821:virology*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; D037521:Virulence Factors; C059731:oncogene protein E7, Human papillomavirus type 16