title: Purified sakacin A shows a dual mechanism of action against  Listeria spp: proton motive force dissipation and cell wall breakdown.
abstract: Sakacin A was purified to homogeneity through simple chromatographic procedures from cultures of Lactobacillus sakei DSMZ 6333 grown on a low-cost medium. The highly purified protein dissipated both transmembrane potential (ΔΨ) and transmembrane pH gradient (ΔpH) in Listeria cells in a very intense, rapid, and energy-dependent fashion. On a slower timescale, purified sakacin A also showed a lytic activity toward isolated cell walls of Listeria. Mass spectrometry was used to analyze the products of sakacin A action on cell walls, evidencing that sakacin A acts on various types of bonds within peptoglycans.
authors: Dipartimento di Scienze Molecolari Agroalimentari, Università degli Studi, Milano, Italy. valentina.trinetta@ecolab.com
authors: Valentina
authors: V
authors: Trinetta
authors: Anna
authors: A
authors: Morleo
authors: Fabio
authors: F
authors: Sessa
authors: Stefania
authors: S
authors: Iametti
authors: Francesco
authors: F
authors: Bonomi
authors: Pasquale
authors: P
authors: Ferranti
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D001430:Bacteriocins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects*; D002845:Chromatography / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D007778:Lactobacillus / Q000378:metabolism; D008087:Listeria / Q000187:drug effects*; D013058:Mass Spectrometry; D010457:Peptidoglycan / Q000032:analysis; D018892:Proton-Motive Force / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D003470:Culture Media; D010457:Peptidoglycan; C071456:sakacin A