title: Akap200 suppresses the effects of Dv-cbl expression in the Drosophila eye.
abstract: The Drosophila                         melanogaster orthologue of the c-Cbl proto-oncogene acts to downregulate signalling from receptor tyrosine kinases by enhancing endocytosis of activated receptors. Expression of an analogue of the C-terminally truncated v-Cbl oncogene, Dv-cbl, in the developing Drosophila eye conversely leads to excess signalling and disruption to the well-ordered adult compound eye. Co-expression of activated Ras with Dv-cbl leads to a severe disruption of eye development. We have used a transposon-based inducible expression system to screen for molecules that can suppress the Dv-cbl phenotype and have identified an allele that upregulates the A-kinase anchoring protein, Akap200. Overexpression of Akap200 not only suppresses the phenotype caused by Dv-cbl expression, but also the severe disruption to eye development caused by the combined expression of Dv-cbl and activated Ras. Akap200 is also endogenously expressed in the developing Drosophila eye at a level that modulates the effects of excessive signalling caused by expression of Dv-cbl.
authors: Department of Anatomy and Neuroscience, University of Melbourne, Parkville, VIC, Australia.
authors: Rowena T
authors: RT
authors: Sannang
authors: Hannah
authors: H
authors: Robertson
authors: Nicole A
authors: NA
authors: Siddall
authors: Gary R
authors: GR
authors: Hime
mesh_terms: D054758:A Kinase Anchor Proteins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster; D005123:Eye / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D050721:Proto-Oncogene Proteins c-cbl / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D054758:A Kinase Anchor Proteins; C121679:Akap200 protein, Drosophila; D029721:Drosophila Proteins; D008565:Membrane Proteins; D050721:Proto-Oncogene Proteins c-cbl; C417283:Ras85D protein, Drosophila; D018631:ras Proteins; C106411:Cbl protein, Drosophila