title: Gelatin methacrylate as a promising hydrogel for 3D microscale organization and proliferation of dielectrophoretically patterned cells.
abstract: Establishing the 3D microscale organization of cells has numerous practical applications, such as in determining cell fate (e.g., proliferation, migration, differentiation, and apoptosis) and in making functional tissue constructs. One approach to spatially pattern cells is by dielectrophoresis (DEP). DEP has characteristics that are important for cell manipulation, such as high accuracy, speed, scalability, and the ability to handle both adherent and non-adherent cells. However, widespread application of this method is largely restricted because there is a limited number of suitable hydrogels for cell encapsulation. To date, polyethylene glycol-diacrylate (PEG-DA) and agarose have been used extensively for dielectric patterning of cells. In this study, we propose gelatin methacrylate (GelMA) as a promising hydrogel for use in cell dielectropatterning because of its biocompatibility and low viscosity. Compared to PEG hydrogels, GelMA hydrogels showed superior performance when making cell patterns for myoblast (C2C12) and endothelial (HUVEC) cells as well as in maintaining cell viability and growth. We also developed a simple and robust protocol for co-culture of these cells. Combined application of the GelMA hydrogels and the DEP technique is suitable for creating highly complex microscale tissues with important applications in fundamental cell biology and regenerative medicine in a rapid, accurate, and scalable manner.
authors: WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan.
authors: Javier
authors: J
authors: Ramón-Azcón
authors: Samad
authors: S
authors: Ahadian
authors: Raquel
authors: R
authors: Obregón
authors: Gulden
authors: G
authors: Camci-Unal
authors: Serge
authors: S
authors: Ostrovidov
authors: Vahid
authors: V
authors: Hosseini
authors: Hirokazu
authors: H
authors: Kaji
authors: Kosuke
authors: K
authors: Ino
authors: Hitoshi
authors: H
authors: Shiku
authors: Ali
authors: A
authors: Khademhosseini
authors: Tomokazu
authors: T
authors: Matsue
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D018920:Coculture Techniques; D004587:Electrophoresis, Agar Gel; D005780:Gelatin / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D008689:Methacrylates; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D005780:Gelatin