title: Shifts in the microbial population in relation to in situ caries progression.
abstract: The shift in microbial diversity from young to mature plaque, related to caries activity on sound and restored surfaces, was studied using denaturing gradient gel electrophoresis. During a 20-week in situ study on caries progression 8 subjects wearing restored and unrestored dentin and enamel sections, biofilm was sampled after 1 and 20 weeks (young or mature plaque). A higher microbial diversity (mature plaque) was seen in caries-active compared to caries-free subjects. Rothia dentocariosa and Scardovia inopinata were absent from all caries-free sites, but appeared in 50% of the caries-active sites.
authors: Center for Dentistry and Oral Hygiene, University Medical Center Groningen, The Netherlands. R.Z.Thomas@umcg.nl
authors: R Z
authors: RZ
authors: Thomas
authors: V
authors: V
authors: Zijnge
authors: A
authors: A
authors: Ciçek
authors: J J
authors: JJ
authors: de Soet
authors: H J M
authors: HJ
authors: Harmsen
authors: M C D N J M
authors: MC
authors: Huysmans
mesh_terms: D000190:Actinomyces / Q000302:isolation & purification; D000191:Actinomycetaceae / Q000302:isolation & purification; D001644:Bifidobacterium / Q000302:isolation & purification; D018441:Biofilms / Q000254:growth & development*; D003731:Dental Caries / Q000382:microbiology*; D003743:Dental Enamel / Q000382:microbiology; D003773:Dental Plaque / Q000382:microbiology*; D003793:Dental Restoration, Permanent; D003804:Dentin / Q000382:microbiology; D018450:Disease Progression; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification; D052197:Limosilactobacillus fermentum / Q000302:isolation & purification; D034361:Streptococcus mitis / Q000302:isolation & purification; D013295:Streptococcus mutans / Q000302:isolation & purification; D019533:Streptococcus oralis / Q000302:isolation & purification; D013298:Streptococcus sanguis / Q000302:isolation & purification; D013499:Surface Properties; D014678:Veillonella / Q000302:isolation & purification