title: Secretion of slow-folding proteins by a Type 1 secretion system.
abstract: Protein production through dedicated secretion systems might offer an potential alternative to the conventional cytoplasmical expression. The application of Type 1 secretion systems of Gram-negative bacteria, however, where often not successful in the past for a wide range of proteins. Recently, two studies using the E. coli maltose binding protein (MalE) and the rat intestinal fatty acid binding protein (IFABP) revealed a rational to circumvent these limitations. Here, wild-type passenger proteins were not secreted, while folding mutants with decreased folding kinetics were efficiently exported to the extracellular space. Subsequently, an one-step purification protocol yielded homogeneous and active protein. Taken together, theses two studies suggest that the introduction of slow-folding mutations into a protein sequence might be the key to use Type 1 secretion systems for the biotechnological production of proteins.
authors: Institute of Biochemistry, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
authors: Christian K W
authors: CK
authors: Schwarz
authors: Michael H H
authors: MH
authors: Lenders
authors: Sander H J
authors: SH
authors: Smits
authors: Lutz
authors: L
authors: Schmitt
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation; D033902:Periplasmic Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D017510:Protein Folding; D058767:Protein Unfolding; D051381:Rats
chemical_list: D029968:Escherichia coli Proteins; D050556:Fatty Acid-Binding Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; C461619:MalE protein, E coli; D033902:Periplasmic Binding Proteins