title: A novel role for FOXA2 and SHH in organizing midbrain signaling centers.
abstract: The floor plate (FP) is a midline signaling center, known to direct ventral cell fates and axon guidance in the neural tube. The recent identification of midbrain FP as a source of dopaminergic neurons has renewed interest in its specification and organization, which remain poorly understood. In this study, we have examined the chick midbrain and spinal FP and show that both can be partitioned into medial (MFP) and lateral (LFP) subdivisions. Although Hedgehog (HH) signaling is necessary and sufficient for LFP specification, it is not sufficient for MFP induction. By contrast, the transcription factor FOXA2 can execute the full midbrain and spinal cord FP program via HH-independent and dependent mechanisms. Interestingly, although HH-independent FOXA2 activity is necessary and sufficient for inducing MFP-specific gene expression (e.g., LMX1B, BMP7), it cannot confer ventral identity to midline cells without also turning on Sonic hedgehog (SHH). We also note that the signaling centers of the midbrain, the FP, roof plate (RP) and the midbrain-hindbrain boundary (MHB) are physically contiguous, with each expressing LMX1B and BMP7. Possibly as a result, SHH or FOXA2 misexpression can transform the MHB into FP and also suppress RP induction. Conversely, HH or FOXA2 knockdown expands the endogenous RP and transforms the MFP into a RP and/or MHB fate. Finally, combined HH blockade and FOXA2 misexpression in ventral midbrain induces LMX1B expression, which triggers the specification of the RP, rather than the MFP. Thus we identify HH-independent and dependent roles for FOXA2 in specifying the FP. In addition, we elucidate for the first time, a novel role for SHH in determining whether a midbrain signaling center will become the FP, MHB or RP.
authors: Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712-0248, USA.
authors: Roy D
authors: RD
authors: Bayly
authors: Charmaine Y
authors: CY
authors: Brown
authors: Seema
authors: S
authors: Agarwala
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000378:metabolism; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism*; D051542:Hepatocyte Nuclear Factor 3-beta / Q000235:genetics / Q000378:metabolism*; D008636:Mesencephalon / Q000196:embryology* / Q000378:metabolism*; D008954:Models, Biological; D015398:Signal Transduction / Q000235:genetics*; D013131:Spine / Q000378:metabolism
chemical_list: D053823:Hedgehog Proteins; C485184:enhanced green fluorescent protein; D051542:Hepatocyte Nuclear Factor 3-beta; D049452:Green Fluorescent Proteins