title: Purification, characterization and antioxidant activities in vitro and in vivo of the polysaccharides from Boletus edulis bull.
abstract: A water-soluble polysaccharide (BEBP) was extracted from Boletus edulis Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and BEBP-3. In the next experiment, the average molecular weight (Mw), IR and monosaccharide compositional analysis of the three polysaccharide fractions were determined. The evaluation of antioxidant activities both in vitro and in vivo suggested that BEBP-3 had good potential antioxidant activity, and should be explored as a novel potential antioxidant.
authors: Department of Landscape Plants, Sichuan Agriculture University, Chengdu 611130, China.
authors: Aoxue
authors: A
authors: Luo
authors: Aoshuang
authors: A
authors: Luo
authors: Jiandong
authors: J
authors: Huang
authors: Yijun
authors: Y
authors: Fan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D052160:Benzothiazoles; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D016166:Free Radical Scavengers / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D010084:Oxidation-Reduction / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D013844:Thiazoles / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D016166:Free Radical Scavengers; D009005:Monosaccharides; D011134:Polysaccharides; D013451:Sulfonic Acids; D013844:Thiazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; D008315:Malondialdehyde; D013482:Superoxide Dismutase