title: [Expression of iNOS in the testis of Fmr1 knockout mice in different developmental stages].
abstract: OBJECTIVE
  To analyze the expression of inducible nitric oxide synthase (iNOS) in the testis tissues of Fmr1 (fragile X mental retardation 1) knockout and wild-type male mice in different developmental stages, and provide background information for researches on fragile X syndrome.
  
  
  METHODS
  This study included 4, 6, 8 and 10 weeks old Fmr1 knockout and wild-type male mice, 6 in each age group. We identified the genotype of the mice by PCR, and detected and compared the expression of iNOS in the testis tissues of the Fmr1 knockout and wild-type mice by immunohistochemistry.
  
  
  RESULTS
  The iNOS expression was weakly positive in the Leydig cells of the 4-week-old mice, moderately positive in the 6-week-old ones, and strongly positive in 8- and 10-week-old ones, significantly weaker in the Fmr1 knockout than in the wild-type ones.
  
  
  CONCLUSIONS
  The expression of iNOS significantly decreases in the testis of Fmr1 knockout mice, suggesting that iNOS may be involved in the pathogenesis of fragile X syndrome.
authors: Department of Reproductive Health Care, Maternity and Child Health Hospital of Longgang District, Shenzhen, Guangdong 518172, China. yingyi19666@yahoo.com.cn
authors: Yi
authors: Y
authors: Yang
authors: Xu-Hong
authors: XH
authors: Liu
authors: Bo
authors: B
authors: Lin
authors: Xu-Dong
authors: XD
authors: Tan
authors: Sheng-Qiang
authors: SQ
authors: Chen
authors: Wei-Wen
authors: WW
authors: Sun
authors: Yue-Ling
authors: YL
authors: Huang
authors: Li-Jun
authors: LJ
authors: Dai
mesh_terms: D000818:Animals; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics*; D005600:Fragile X Syndrome / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
chemical_list: D051860:Fragile X Messenger Ribonucleoprotein 1; D052247:Nitric Oxide Synthase Type II; C497684:Fmr1 protein, mouse; C496317:Nos2 protein, mouse