title: Proteomic profiling of skeletal muscle in an animal model of overtraining.
abstract: Excessive training (i.e. overtraining, OT) may result in underperformance, which can be characterized by the time needed to re-establish performance (i.e. functional overreaching (FOR), nonfunctional overreaching, OT syndrome). The present study is an initial screening for proteins presenting altered abundance in the red (RG) and white (WG) portions of the gastrocnemius muscle from rats submitted to an OT protocol that induced FOR. In the RG, compared to the nontrained control, FOR demonstrated an increased abundance of proteins normally related to adaptation to endurance training (e.g. proteins of oxidative phosphorylation complexes, proteins related to lipid metabolism, antioxidants, and chaperones). In the WG, spots identified as mitochondrial aconitase and a component of the succinate dehydrogenase complex were downregulated in FOR, as were proteins related to myofibril stabilization; these latter were upregulated in the RG. This initial study shows that skeletal muscles with different fiber-type compositions respond differently to an OT period. Also, it is likely that actin-interacting proteins have an important role in muscle adaptation to endurance exercise.
authors: Laboratory of Biochemistry of Exercise, UNICAMP, Campinas, Brazil.
authors: Paulo Guimaraes
authors: PG
authors: Gandra
authors: Richard Hemmi
authors: RH
authors: Valente
authors: Jonas
authors: J
authors: Perales
authors: Antonio Guilherme Fonseca
authors: AG
authors: Pacheco
authors: Denise Vaz
authors: DV
authors: Macedo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology*; D018657:Muscle Fibers, Slow-Twitch / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000378:metabolism; D010805:Physical Conditioning, Animal; D005082:Physical Exertion; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D011506:Proteins; D017382:Reactive Oxygen Species; D018995:Myosin Heavy Chains