title: Chimeric viruses containing the N-terminal ectodomains of GP5 and M proteins of porcine reproductive and respiratory syndrome virus do not change the cellular tropism of equine arteritis virus.
abstract: Equine arteritis virus (EAV) and porcine reproductive and respiratory syndrome virus (PRRSV) are members of family Arteriviridae; they are highly species specific and differ significantly in cellular tropism in cultured cells. In this study we examined the role of the two major envelope proteins (GP5 and M) of EAV and PRRSV in determining their cellular tropism. We generated three viable EAV/PRRSV chimeric viruses by swapping the N-terminal ectodomains of these two proteins from PRRSV IA1107 strain into an infectious cDNA clone of EAV (rMLVB4/5 GP5ecto, rMLVB4/5/6 Mecto and rMLVB4/5/6 GP5&Mecto). The three chimeric viruses could only infect EAV susceptible cell lines but not PRRSV susceptible cells in culture. Therefore, these data unequivocally demonstrate that the ectodomains of GP5 and M are not the major determinants of cellular tropism, further supporting the recent findings that the minor envelope proteins are the critical proteins in mediating cellular tropism (Tian et al., 2012).
authors: Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546, United States.
authors: Zhengchun
authors: Z
authors: Lu
authors: Jianqiang
authors: J
authors: Zhang
authors: Chengjin M
authors: CM
authors: Huang
authors: Young Yun
authors: YY
authors: Go
authors: Kay S
authors: KS
authors: Faaberg
authors: Raymond R R
authors: RR
authors: Rowland
authors: Peter J
authors: PJ
authors: Timoney
authors: Udeni B R
authors: UB
authors: Balasuriya
mesh_terms: D000818:Animals; D002460:Cell Line; D018076:DNA, Complementary; D007240:Equartevirus / Q000235:genetics / Q000502:physiology*; D006736:Horses; D008969:Molecular Sequence Data; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013552:Swine; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D056189:Viral Tropism
chemical_list: D018076:DNA, Complementary; C525999:M protein, porcine reproductive and respiratory syndrome virus; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; C114789:glycoprotein 5, PRRSV