title: Increased production of IL-5 and dominant Th2-type response in airways of Churg-Strauss syndrome patients.
abstract: OBJECTIVE
  Churg-Strauss syndrome (CSS) is a rare systemic vasculitis associated with eosinophilia and asthma. We assessed the local immune response in airways of CSS patients with different activity of the disease.
  
  
  METHODS
  Concentration of IL-5, CCL17, CCL22 and CCL26 (ELISA) together with cell expression of T-helper-related genes (real-time PCR array) were measured in bronchoalveolar lavage fluid (BALF) sampled from 11 patients with active CSS, 11 patients with CSS in remission and 9 control subjects with bronchial asthma.
  
  
  RESULTS
  In active CSS, both BALF and blood eosinophil counts were increased (P<0.01). BALF cells in active disease were characterized by an increased expression of Th2 and regulatory-type transcripts: STAT6, STAT3, GATA3, IL4, IL5 and IL10 as compared with asthmatics, and STAT5A, CCR4, FOXP3, IL4, IL5 and IL10 when compared with inactive CSS. There was significant increase in BALF concentration of IL-5 and CCL26 in exacerbation of CSS. CCR4-active chemokines were detected more frequently in active disease. We found a strong positive correlation between clinical parameters of disease activity (BVAS, eosinophilia) and expression of IL4, IL5, IL10 and STAT5A.
  
  
  CONCLUSIONS
  These results indicate that as compared with asthma, active-CSS patients have much stronger local Th2 response in the airways. Airway cells may contribute to lung eosinophilia in CSS by producing IL-5 and eosinophil active chemokines.
authors: Department of Medicine, Jagiellonian University Medical College, ul. Skawinska 8, 31-066 Krakow, Poland.
authors: Bogdan
authors: B
authors: Jakiela
authors: Wojciech
authors: W
authors: Szczeklik
authors: Hanna
authors: H
authors: Plutecka
authors: Barbara
authors: B
authors: Sokolowska
authors: Lucyna
authors: L
authors: Mastalerz
authors: Marek
authors: M
authors: Sanak
authors: Stanislawa
authors: S
authors: Bazan-Socha
authors: Andrzej
authors: A
authors: Szczeklik
authors: Jacek
authors: J
authors: Musial
mesh_terms: D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000276:immunology; D054414:Chemokine CCL17 / Q000032:analysis; D054422:Chemokine CCL22 / Q000032:analysis; D000075906:Chemokine CCL26; D019742:Chemokines, CC / Q000032:analysis; D015267:Churg-Strauss Syndrome / Q000276:immunology* / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology; D005260:Female; D015870:Gene Expression / Q000276:immunology*; D006801:Humans; D015848:Interleukin-5 / Q000032:analysis*; D008297:Male; D008875:Middle Aged
chemical_list: C120429:CCL26 protein, human; D054414:Chemokine CCL17; D054422:Chemokine CCL22; D000075906:Chemokine CCL26; D019742:Chemokines, CC; D015848:Interleukin-5