title: An atomic-force basis for the bacteriolytic effects of granulysin.
abstract: While granulysin has been suggested to play an important role in adaptive immune responses against bacterial infections by killing pathogens, and molecular force for protein-protein interaction or protein-bacteria interaction may designate the specific functions of a protein, the molecular-force basis underlying the bacteriolytic effects of granulysin at single-molecule level remains unknown. Here, we produced and purified bactericidal domain of macaque granulysin (GNL). Our bacterial lysis assays suggested that GNL could efficiently kill bacteria such as Listeria monocytogenes. Furthermore, we found that the interaction force between GNL and L. monocytogenes measured by an atomic force microscopy (AFM) was about 22.5 pN. Importantly, our AFM-based single molecular analysis suggested that granulysin might lyse the bacteria not only through electrostatic interactions but also by hydrogen bonding and van der Waals interaction. Thus, this work provides a previous unknown mechanism for bacteriolytic effects of granulysin.
authors: Department of Chemistry, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
authors: Yueqin
authors: Y
authors: Qiu
authors: An-Bin
authors: AB
authors: Hu
authors: Huiyong
authors: H
authors: Wei
authors: Hongying
authors: H
authors: Liao
authors: Shaoyuan
authors: S
authors: Li
authors: Crystal Y
authors: CY
authors: Chen
authors: Weihua
authors: W
authors: Zhong
authors: Dan
authors: D
authors: Huang
authors: Jiye
authors: J
authors: Cai
authors: Lifang
authors: L
authors: Jiang
authors: Gucheng
authors: G
authors: Zeng
authors: Zheng W
authors: ZW
authors: Chen
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry* / Q000494:pharmacology; D001433:Bacteriolysis; D018914:Cells, Immobilized; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000235:genetics; D006860:Hydrogen Bonding; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008251:Macaca; D018625:Microscopy, Atomic Force; D054353:Perforin / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D055672:Static Electricity
chemical_list: D000538:Aluminum Silicates; D000900:Anti-Bacterial Agents; D000945:Antigens, Differentiation, T-Lymphocyte; D011994:Recombinant Proteins; D054353:Perforin; C011934:mica