title: Investigation of quadruplex structure under physiological conditions using in-cell NMR.
abstract: In this chapter we describe the application of in-cell NMR spectroscopy to the investigation of G-quadruplex structures inside living Xenopus laevis oocytes and in X. laevis egg extract. First, in-cell NMR spectroscopy of nucleic acids (NA) is introduced and applications and limitations of the approach are discussed. In the following text the application of in-cell NMR spectroscopy to investigation of G-quadruplexes are reviewed. Special emphasis is given to the discussion of the influence of the intracellular environmental factors such as low molecular weight compounds, molecular crowding, and hydration on structural behavior of G-quadruplexes. Finally, future perspectives of in-cell NMR spectroscopy for quantitative characterization of G-quadruplexes and NA are discussed.
authors: Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt/Main, Germany.
authors: Robert
authors: R
authors: Hänsel
authors: Silvie
authors: S
authors: Foldynová-Trantírková
authors: Volker
authors: V
authors: Dötsch
authors: Lukáš
authors: L
authors: Trantírek
mesh_terms: D000818:Animals; D054856:G-Quadruplexes; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis*; D009865:Oocytes / Q000737:chemistry / Q000378:metabolism; D014982:Xenopus laevis / Q000235:genetics
chemical_list: D009696:Nucleic Acids