title: A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells.
abstract: We present the design, synthesis, spectroscopy, and biological applications of PyBor, a new type of fluorescent probe for peroxynitrite detection in aqueous solution and living cells. The probe employs pyrene as the fluorophore, and is equipped with a chemically responsive unit boronate. The fluorescent probe can selectively detect peroxynitrite with fluorimetric determination and high-performance liquid chromatography analyses in aqueous solution and RAW264.7 cells intracellular free extracts. We also study our probe to time dependent peroxynitrite release from 3-morpholinylsydnonimine hydrochloride. Confocal microscopy experiments using mouse macrophage cell line RAW264.7 show that PyBor is able to detect the different intracellular peroxynitrite levels. In addition, we have performed quantum chemical calculations with TD-DFT/M06/TZVP level with COSMO solvation model basis sets using a suite of Gaussian 09 programs to provide insights into the structure optical properties of PyBor and PyOH.
authors: State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR China.
authors: Fabiao
authors: F
authors: Yu
authors: Ping
authors: P
authors: Song
authors: Peng
authors: P
authors: Li
authors: Bingshuai
authors: B
authors: Wang
authors: Keli
authors: K
authors: Han
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000737:chemistry*; D042541:Intracellular Space / Q000378:metabolism; D057230:Limit of Detection; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D010084:Oxidation-Reduction; D030421:Peroxynitrous Acid / Q000378:metabolism*
chemical_list: D001897:Boronic Acids; D005456:Fluorescent Dyes; D030421:Peroxynitrous Acid