title: Expression of selected Ginkgo biloba heat shock protein genes after cold treatment could be induced by other abiotic stress.
abstract: Heat shock proteins (HSPs) play various stress-protective roles in plants. In this study, three HSP genes were isolated from a suppression subtractive hybridization (SSH) cDNA library of Ginkgo biloba leaves treated with cold stress. Based on the molecular weight, the three genes were designated GbHSP16.8, GbHSP17 and GbHSP70. The full length of the three genes were predicted to encode three polypeptide chains containing 149 amino acids (Aa), 152 Aa, and 657 Aa, and their corresponding molecular weights were predicted as follows: 16.67 kDa, 17.39 kDa, and 71.81 kDa respectively. The three genes exhibited distinctive expression patterns in different organs or development stages. GbHSP16.8 and GbHSP70 showed high expression levels in leaves and a low level in gynoecia, GbHSP17 showed a higher transcription in stamens and lower level in fruit. This result indicates that GbHSP16.8 and GbHSP70 may play important roles in Ginkgo leaf development and photosynthesis, and GbHSP17 may play a positive role in pollen maturation. All three GbHSPs were up-regulated under cold stress, whereas extreme heat stress only caused up-regulation of GbHSP70, UV-B treatment resulted in up-regulation of GbHSP16.8 and GbHSP17, wounding treatment resulted in up-regulation of GbHSP16.8 and GbHSP70, and abscisic acid (ABA) treatment caused up-regulation of GbHSP70 primarily.
authors: Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratory, Huanggang Normal University, Huanggang 438000, China.|College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China.
authors: Fuliang
authors: F
authors: Cao
authors: Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratory, Huanggang Normal University, Huanggang 438000, China.|College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China.|College of Chemistry and life science, Huanggang Normal University, Huanggang 438000, China.
authors: Hua
authors: H
authors: Cheng
authors: Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratory, Huanggang Normal University, Huanggang 438000, China.|College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China.|College of Chemistry and life science, Huanggang Normal University, Huanggang 438000, China.
authors: Shuiyuan
authors: S
authors: Cheng
authors: Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratory, Huanggang Normal University, Huanggang 438000, China.|College of Chemistry and life science, Huanggang Normal University, Huanggang 438000, China.
authors: Linling
authors: L
authors: Li
authors: Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratory, Huanggang Normal University, Huanggang 438000, China.
authors: Feng
authors: F
authors: Xu
authors: College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China.
authors: Wanwen
authors: W
authors: Yu
authors: College of Chemistry and life science, Huanggang Normal University, Huanggang 438000, China.
authors: Honghui
authors: H
authors: Yuan
mesh_terms: D000595:Amino Acid Sequence; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D020441:Ginkgo biloba / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D013312:Stress, Physiological
chemical_list: D006360:Heat-Shock Proteins; D010940:Plant Proteins