title: Experimental murine model of primary high grade undifferentiated pleomorphic sarcoma not otherwise specified.
abstract: BACKGROUND
  Undifferentiated pleomorphic sarcoma not otherwise specified (NOS) is a malignant neoplasm of uncertain origin arising both in the soft tissue and the bone. The WHO classified this tumour in 2002 but controversy has plagued this entity due to limited availability of tissue for study. The aim of this study was to establish a reproducible xenograft model of primary human undifferentiated pleomorphic sarcoma NOS.
  
  
  METHODS
  Primary human sarcoma samples were divided into tumour fragments and transplanted subcutaneously in mice. Sarcoma xenografts were analysed histolomorphologically (light/electron-microscopy; immunohistochemistry).
  
  
  RESULTS
  All tumours resulted in viable sarcoma NOS xenografts demonstrating similar histological patterns. In both the original tumours and the xenografts, tumour necrosis was found ranging from 15% to 25%. The background stroma of the xenografts was hyalinised like the primary sarcoma. Electron microscopical analyses showed good maintenance of ultrastructure.
  
  
  CONCLUSIONS
  Implantation of intact tumor fragments yielded in a complete tumor take rate. The development of new cancer therapeutics requires animal models that closely resemble the human patient. This study provides ideal animal models for the research of pathogenesis and pathobiology of primary human undifferentiated pleomorphic sarcoma NOS.
authors: Reference Centre for Soft Tissue Sarcoma, Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr University Bochum, Bürkle-de-la-Camp-Platz 1, 44789 Bochum, North Rhine-Westphalia, Germany. d.tilkorn@web.de
authors: Daniel J
authors: DJ
authors: Tilkorn
authors: Ingo
authors: I
authors: Stricker
authors: Joerg
authors: J
authors: Hauser
authors: Andrej
authors: A
authors: Ring
authors: Inge
authors: I
authors: Schmitz
authors: Lars
authors: L
authors: Steinstraesser
authors: Hans-Ulrich
authors: HU
authors: Steinau
authors: Adrien
authors: A
authors: Daigeler
authors: Sammy
authors: S
authors: Al-Benna
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008819:Mice, Nude; D012513:Sarcoma, Experimental / Q000473:pathology*