title: Competitive incorporation of homologous gene segments of influenza A virus into virions.
abstract: By using two reporter protein-encoding virus-like RNAs derived from identical viral RNA (vRNA) segments, we assessed their incorporation efficiency into single progeny virions. Most plaques formed by the recombinant viruses that were generated in cells positive for both reporter genes expressed only one or the other protein. These results suggest that two virus-like RNAs encoding different reporter proteins compete for incorporation into virions, and individual influenza virions incorporate single, but not multiple, copies of homologous vRNA segments.
authors: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Shirokanedai, Tokyo, Japan.
authors: Arisa
authors: A
authors: Inagaki
authors: Hideo
authors: H
authors: Goto
authors: Satoshi
authors: S
authors: Kakugawa
authors: Makoto
authors: M
authors: Ozawa
authors: Yoshihiro
authors: Y
authors: Kawaoka
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D017930:Genes, Reporter; D005814:Genes, Viral; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000502:physiology; D009439:Neuraminidase / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics; D014771:Virion / Q000235:genetics / Q000502:physiology; D019065:Virus Assembly / Q000235:genetics / Q000502:physiology
chemical_list: C057027:PB2 protein, Influenzavirus A; D012367:RNA, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase; C487630:NA protein, influenza A virus; D009439:Neuraminidase