title: Structural and SAXS analysis of the budding yeast SHU-complex proteins.
abstract: In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8Å resolution and successfully fitted into our small angle X-ray scattering (SAXS) data of the SHU-complex. Taken together with our electrophoretic mobility shift assay (EMSA) results, a model is proposed for the SHU-protein complex coupled with DNA.
authors: Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
authors: Zhun
authors: Z
authors: She
authors: Zeng-Qiang
authors: ZQ
authors: Gao
authors: Ying
authors: Y
authors: Liu
authors: Wen-Jia
authors: WJ
authors: Wang
authors: Guang-Feng
authors: GF
authors: Liu
authors: Eleonora V
authors: EV
authors: Shtykova
authors: Jian-Hua
authors: JH
authors: Xu
authors: Yu-Hui
authors: YH
authors: Dong
mesh_terms: D003001:Cloning, Molecular; D018360:Crystallography, X-Ray / Q000379:methods; D004247:DNA / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D059765:Homologous Recombination; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000502:physiology*; D011485:Protein Binding; D011487:Protein Conformation; D025941:Protein Interaction Mapping; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000502:physiology*; D012542:Scattering, Radiation
chemical_list: D005656:Fungal Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; C562223:Shu1 protein, S cerevisiae; D004247:DNA