title: Multilocus definition of MHC haplotypes in pedigreed cynomolgus macaques (Macaca fascicularis).
abstract: Cynomolgus macaques (Macaca fascicularis) are used widely in biomedical research, and the genetics of their MHC (Mhc-Mafa) has become the focus of considerable attention in recent years. The cohort of Indonesian pedigreed macaques that we present here was typed for Mafa-A, -B, and -DR, by sequencing, as described in earlier studies. Additionally, the DRB region of these animals was characterised by microsatellite analyses. In this study, full-length sequencing of Mafa-DPA/B and -DQA/B in these animals was performed. A total of 75 different alleles were observed; 22 of which have not previously been reported, plus 18 extended exon 2 alleles that were already known. Furthermore, two microsatellites, D6S2854 and D6S2859, were used to characterise the complex Mafa-A region. Sequencing and segregation analyses revealed that the length patterns of these microsatellites are unique for each Mafa-A haplotype. In this work, we present a pedigreed colony of approximately 120 cynomolgus macaques; all of which are typed for the most significant polymorphic MHC class I and class II markers. Offspring of these pedigreed animals are easily characterised for their MHC by microsatellite analyses on the Mafa-A and -DRB regions, which makes the cumbersome sequencing analyses redundant.
authors: Department of Comparative Genetics and Refinement, Biomedical Primate Research Centre, Lange Kleiweg 161, 2288GJ, Rijswijk, The Netherlands. otting@bprc.nl
authors: Nel
authors: N
authors: Otting
authors: Nanine
authors: N
authors: de Groot
authors: Annemiek J M
authors: AJ
authors: de Vos-Rouweler
authors: Annet
authors: A
authors: Louwerse
authors: Gaby G M
authors: GG
authors: Doxiadis
authors: Ronald E
authors: RE
authors: Bontrop
mesh_terms: D000483:Alleles; D000818:Animals; D005260:Female; D005805:Genes, MHC Class I / Q000235:genetics*; D005802:Genes, MHC Class II / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics*; D008252:Macaca fascicularis / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D010375:Pedigree