title: Hormone profiling in a novel enteroendocrine cell line pGIP/neo: STC-1.
abstract: OBJECTIVE
  GIP is a peptide hormone of therapeutic interest in type 2 diabetes and obesity. This study evaluated pGIP/neo STC-1 as a potential K-cell model for studying GIP secretion.
  
  
  METHODS
  We evaluated cellular storage and medium accumulation of GIP along with other gastrointestinal peptides cholecystokinin (CCK), peptide YY (PYY), obestatin and ghrelin over 72 h and probed possible intracellular signals (PKA, PKC, Ca(2+) and GPCR) involved in peptide hormone synthesis/secretion.
  
  
  RESULTS
  Results demonstrate for the first time that pGIP/Neo STC-1 cells produce and secrete 3 to 6 times more GIP than STC-1. The cells clearly retain the ability to synthesize and secrete CCK and PYY but reduced levels indicate a shift towards a predominantly K-cell phenotype. Furthermore, gastric peptides such as obestatin and ghrelin are not produced in either STC-1 or pGIP/Neo STC-1 cells.
  
  
  CONCLUSIONS
  This study demonstrates the potential usefulness of pGIP/Neo cells for studying GIP secretion and further investigations will establish its suitability for investigating hormone release in vitro.
authors: Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University Belfast, David Keir Building, Stranmillis Road, Belfast BT9 5AG, UK.
authors: Katharine V
authors: KV
authors: Hand
authors: Linda
authors: L
authors: Giblin
authors: Brian D
authors: BD
authors: Green
mesh_terms: D002460:Cell Line; D002766:Cholecystokinin / Q000378:metabolism*; D019858:Enteroendocrine Cells / Q000378:metabolism*; D005749:Gastric Inhibitory Polypeptide / Q000378:metabolism*; D005768:Gastrointestinal Hormones / Q000378:metabolism; D054439:Ghrelin / Q000378:metabolism*; D006801:Humans; D019894:Peptide YY / Q000378:metabolism*
chemical_list: D005768:Gastrointestinal Hormones; D054439:Ghrelin; D019894:Peptide YY; D005749:Gastric Inhibitory Polypeptide; D002766:Cholecystokinin