title: Collagen I and III and metalloproteinase gene and protein expression in prostate cancer in relation to Gleason score.
abstract: OBJECTIVE
  To evaluate if the expression of metalloproteinase, collagen I and III are related to Gleason score, preoperative PSA and pathological stage in prostate cancer.
  
  
  METHODS
  Our study group included radical prostatectomy specimens of 33 patients with prostatic adenocarcinoma who underwent surgery from 2001 to 2009. Patients were divided into 3 groups: Gleason score=6 (13 patients), Gleason score=7 (10 patients), Gleason score ≥ 8 (10 patients). The control group included prostates of patients submitted to cystoprostatectomy and benign prostatic tissues adjacent to the cancer area. Specific areas of tissues were selected under microscope and further processed for collagen I and III analysis by real time PCR. In addition, 10 deparaffined sections of each group were used to evaluate collagen I, III and metalloproteinase immune expression. The results were correlated with Gleason score, preoperative PSA and pathological stage.
  
  
  RESULTS
  We found significant difference in both collagen I and III gene expression between benign and tumoral areas in the prostate samples from Gleason score=6 (collagen I=0.4 ± 0.2 vs 5 ± 2.4, p < 0.05; collagen III=0.2 ± 0.06 vs 0.7 ± 0.1, p < 0.05) and Gleason score ≥ 8 (collagen I=8 ± 3.4 vs 1.4 ± 0.8, p < 0.07; collagen III=1.8 ± 0.5 vs 0.6 ± 0.1, p < 0.05). There was no correlation of collagen expression with Gleason score, preoperative PSA or pathological stage. There was a positive correlation between metalloproteinase expression and Gleason score (r(2)=0.47).
  
  
  CONCLUSIONS
  The positive correlation between metalloproteinase expression and Gleason score suggests that metalloproteinase could be a promising factor to improve Gleason score evaluation. Its expression and regulation do not seem to be related with collagen degradation.
authors: Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Antonio H
authors: AH
authors: Duarte
authors: Sicilia
authors: S
authors: Colli
authors: Jorge L
authors: JL
authors: Alves-Pereira
authors: Max P
authors: MP
authors: Martins
authors: Francisco J B
authors: FJ
authors: Sampaio
authors: Cristiane F
authors: CF
authors: Ramos
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism*; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D045726:Metalloproteases / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D017430:Prostate-Specific Antigen / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D013997:Time Factors
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D045726:Metalloproteases; D017430:Prostate-Specific Antigen