title: Giardia duodenalis sub-Assemblage of animal and human origin in horses.
abstract: In order to evaluate infection occurrence and the potential zoonotic role of horse isolates of Giardia duodenalis, 431 individual fecal samples were genetically characterized by PCR tests -coupled sequencing and phylogenetic analysis. Thirty-seven (8.6%) animals resulted infected by different Assemblage. The presence of sub-Assemblage was assessed by characterizing the β-giardin gene for 16 of the 37 positive horses. Ten isolates showed 99.6% to 100% homology with the sub-Assemblage described as B1-2 and B1-6, three Assemblage A showed 99.8% homology with sub-Assemblage A1, while one Assemblage E displayed 98.8% homology with sub-Assemblage E3. Furthermore, one isolate characterized as Assemblage A showed 99.6% homology with the sub-Assemblage B1-2 and one characterized as E was 100% identical with sub-Assemblage B1-6. These results demonstrate the presence of both animal and human sub-Assemblage of G. duodenalis in horses from Italy. Epidemiological and sanitary implications are discussed.
authors: Department of Comparative Biomedical Sciences, University of Teramo, Italy. dtraversa@unite.it
authors: Donato
authors: D
authors: Traversa
authors: Domenico
authors: D
authors: Otranto
authors: Piermarino
authors: P
authors: Milillo
authors: Maria Stefania
authors: MS
authors: Latrofa
authors: Annunziata
authors: A
authors: Giangaspero
authors: Angela
authors: A
authors: Di Cesare
authors: Barbara
authors: B
authors: Paoletti
mesh_terms: D000818:Animals; D003598:Cytoskeletal Proteins / Q000235:genetics; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry; D005243:Feces / Q000469:parasitology; D060005:Genotyping Techniques; D016829:Giardia lamblia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology* / Q000662:veterinary*; D006736:Horses; D006801:Humans; D007558:Italy / Q000453:epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D015047:Zoonoses
chemical_list: D003598:Cytoskeletal Proteins; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C038207:giardin protein, Giardia lamblia