title: Exploratory investigation of eight circulating plasma markers in brain tumor patients.
abstract: Several blood biomarkers have been established for the early diagnosis, screening and follow-up of non central nervous system cancers. However, there is lack of knowledge on biochemical blood alterations in brain tumor patients. In this study, we prospectively collected blood plasma samples of 105 adult brain tumor patients with diffuse low-grade glioma (World Health Organization (WHO) II, n = 7), anaplastic glioma (WHO III, n = 10), glioblastoma multiforme (WHO IV, glioblastoma multiforme (GBM)) (n = 34), meningioma (WHO I, n = 8), atypical meningioma (WHO II, n = 5), and intracerebral metastasis (ICM; n = 41). In each case, we measured plasma concentrations of neuropeptide Y, brain-derived neurotrophic factor, glial cell line-derived neurotrophic factor, placental growth factor (PlGF), S100B, secretagogin, interleukin 8, and glial fibrillary acidic protein (GFAP) using enzyme-linked immunosorbent assay. Plasma marker concentrations were correlated to patient parameters including neuropathological diagnosis and neuroradiological features. Most of the markers were detectable in all diagnostic categories in variable concentrations. GFAP plasma detectability was strongly associated with a diagnosis of GBM (p < 0.001). Plasma GFAP and plasma placental growth factor showed promising moderate potential in the differential diagnosis of unifocal GBM versus unifocal supratentorial ICM (area under the curve = 0.73, p < 0.05). To summarize, our data show that none of the investigated markers is suitable to substitute histological diagnosis. However, measurement of circulating GFAP and PlGF may support neuroradiological differential diagnosis of GBM versus ICM.
authors: Department of Medicine III, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
authors: Aysegul
authors: A
authors: Ilhan-Mutlu
authors: Ludwig
authors: L
authors: Wagner
authors: Georg
authors: G
authors: Widhalm
authors: Adelheid
authors: A
authors: Wöhrer
authors: Sophie
authors: S
authors: Bartsch
authors: Thomas
authors: T
authors: Czech
authors: Harald
authors: H
authors: Heinzl
authors: Fritz
authors: F
authors: Leutmezer
authors: Daniela
authors: D
authors: Prayer
authors: Christine
authors: C
authors: Marosi
authors: Wolfgang
authors: W
authors: Base
authors: Matthias
authors: M
authors: Preusser
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000737:chemistry; D001774:Blood Chemical Analysis; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology; D003627:Data Interpretation, Statistical; D003937:Diagnosis, Differential; D005260:Female; D005910:Glioma / Q000097:blood / Q000175:diagnosis; D006801:Humans; D053208:Kaplan-Meier Estimate; D016015:Logistic Models; D008279:Magnetic Resonance Imaging; D008297:Male; D008579:Meningioma / Q000097:blood / Q000175:diagnosis; D008875:Middle Aged; D008970:Molecular Weight; D009103:Multiple Sclerosis / Q000097:blood; D019635:Neurosurgical Procedures; D011237:Predictive Value of Tests; D011446:Prospective Studies; D015203:Reproducibility of Results; D016019:Survival Analysis; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor