title: In silico study of interaction between rice proteins enhanced disease susceptibility 1 and  phytoalexin deficient 4, the regulators of salicylic acid signalling pathway.
abstract: Enhanced disease susceptibility 1 (EDS1), a plant-specific protein has homology with the eukaryotic lipase in their N-terminal halves and a unique domain at its C-termini. EDS1 is known to be an important regulator of biotic stress and an essential component of basal immunity. EDS1 interacts with its positive co-regulator phytoalexin deficient 4 (PAD4), resulting in mobilization of the salicylic acid defence pathway. Limited information regarding this interaction in rice is available. To study this interaction, a model of EDS1 and PAD4 proteins from rice was generated and validated with Accelrys DS software version 3.1 using bioinformatics interface. The in silico docking between the two proteins showed a significant protein-protein interaction between rice EDS1 and PAD4, suggesting that they form a dimeric protein complex, which, similar to that in Arabidopsis, is perhaps also important for triggering the salicylic acid signalling pathway in plants.
authors: Department of Bioinformatics, MMV, Banaras Hindu University, Varanasi 221 005, India.
authors: Indra
authors: I
authors: Singh
authors: Kavita
authors: K
authors: Shah
mesh_terms: D000595:Amino Acid Sequence; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D003198:Computer Simulation; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012275:Oryza; D057865:Plant Immunity; D010940:Plant Proteins / Q000737:chemistry*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D020156:Salicylic Acid / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D015398:Signal Transduction; D040681:Structural Homology, Protein
chemical_list: D004268:DNA-Binding Proteins; D010940:Plant Proteins; D002265:Carboxylic Ester Hydrolases; D020156:Salicylic Acid