title: Divergent effects of urban particulate air pollution on allergic airway responses in experimental asthma: a comparison of field exposure studies.
abstract: BACKGROUND
  Increases in ambient particulate matter of aerodynamic diameter of 2.5 μm (PM2.5) are associated with asthma morbidity and mortality. The overall objective of this study was to test the hypothesis that PM2.5 derived from two distinct urban U.S. communities would induce variable responses to aggravate airway symptoms during experimental asthma.
  
  
  METHODS
  We used a mobile laboratory to conduct community-based inhalation exposures to laboratory rats with ovalbumin-induced allergic airways disease. In Grand Rapids exposures were conducted within 60 m of a major roadway, whereas the Detroit was located in an industrial area more than 400 m from roadways. Immediately after nasal allergen challenge, Brown Norway rats were exposed by whole body inhalation to either concentrated air particles (CAPs) or filtered air for 8 h (7:00 AM - 3:00 PM). Both ambient and concentrated PM2.5 was assessed for mass, size fractionation, and major component analyses, and trace element content. Sixteen hours after exposures, bronchoalveolar lavage fluid (BALF) and lung lobes were collected and evaluated for airway inflammatory and mucus responses.
  
  
  RESULTS
  Similar CAPs mass concentrations were generated in Detroit (542 μg/m3) and Grand Rapids (519 μg/m3). Exposure to CAPs at either site had no effects in lungs of non-allergic rats. In contrast, asthmatic rats had 200% increases in airway mucus and had more BALF neutrophils (250% increase), eosinophils (90%), and total protein (300%) compared to controls. Exposure to Detroit CAPs enhanced all allergic inflammatory endpoints by 30-100%, whereas inhalation of Grand Rapids CAPs suppressed all allergic responses by 50%. Detroit CAPs were characterized by high sulfate, smaller sized particles and were derived from local combustion sources. Conversely Grand Rapids CAPs were derived primarily from motor vehicle sources.
  
  
  CONCLUSIONS
  Despite inhalation exposure to the same mass concentration of urban PM2.5, disparate health effects can be elicited in the airways of sensitive populations such as asthmatics. Modulation of airway inflammatory and immune responses is therefore dependent on specific chemical components and size distributions of urban PM2.5. Our results suggest that air quality standards based on particle speciation and sources may be more relevant than particle mass to protect human health from PM exposure.
authors: Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA. wagnerja@msu.edu
authors: James G
authors: JG
authors: Wagner
authors: Masako
authors: M
authors: Morishita
authors: Gerald J
authors: GJ
authors: Keeler
authors: Jack R
authors: JR
authors: Harkema
mesh_terms: D000397:Air Pollution / Q000009:adverse effects* / Q000032:analysis; D000818:Animals; D001249:Asthma / Q000209:etiology* / Q000473:pathology; D016535:Bronchial Hyperreactivity / Q000209:etiology / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D004784:Environmental Monitoring / Q000379:methods; D006801:Humans; D019570:Inhalation Exposure / Q000009:adverse effects* / Q000032:analysis; D008168:Lung / Q000473:pathology; D010316:Particle Size; D052638:Particulate Matter / Q000009:adverse effects* / Q000032:analysis; D051381:Rats; D011914:Rats, Inbred BN; D020545:Respiratory Mucosa / Q000473:pathology; D001335:Vehicle Emissions / Q000633:toxicity
chemical_list: D052638:Particulate Matter; D001335:Vehicle Emissions