title: The proteoglycan repertoire of lymphoid cells.
abstract: Proteoglycans have been studied to a limited extent in lymphoid cells. In this study we have investigated the expression of proteoglycans in B-cells, CD4+ T-cells, CD8+ T-cells, natural killer cells, as well as in nine different cell lines established from patients with lymphoid malignancies. Serglycin was the major proteoglycan expressed at mRNA level by the primary lymphocytes. None of the syndecans or glycpicans was detected at mRNA level in the primary lymphocytes, except for syndecan-4 in CD4+ T-cells and CD8+ T-cells. All lymphoid cell lines expressed serglycin mRNA, as well as one or several members of the syndecan and glypican families. Further, increased synthesis of proteoglycans was found in the cell lines compared to the primary lymphocytes, as well as the presence of heparan sulfate on the cell surface of five of the cells lines. Western blot analysis showed a close correlation between serglycin mRNA level and expression of serglycin core protein. Our results show that serglycin is a major proteoglycan in all the normal lymphoid cells and that these cells carry little, or none, proteoglycans on the cell surface. Serglycin was also a major proteoglycan in the malignant lymphoid cells, but these also expressed one or more types of cell surface proteoglycans. Thus, malignant transformation of lymphoid cells may be followed by increased synthesis of proteoglycans and expression of cell surface proteoglycans.
authors: Institute of Medical Biology, Faculty of Health Sciences, University of Tromsø, Tromsø, Norway. bodil.i.fadnes@uit.no
authors: Bodil
authors: B
authors: Fadnes
authors: Anne
authors: A
authors: Husebekk
authors: Gunbjørg
authors: G
authors: Svineng
authors: Øystein
authors: Ø
authors: Rekdal
authors: Masaki
authors: M
authors: Yanagishita
authors: Svein O
authors: SO
authors: Kolset
authors: Lars
authors: L
authors: Uhlin-Hansen
mesh_terms: D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D019337:Hematologic Neoplasms / Q000378:metabolism* / Q000473:pathology; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D053671:Syndecan-4 / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D006497:Heparan Sulfate; D009363:Neoplasm Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D053671:Syndecan-4; D033921:Vesicular Transport Proteins; C508304:SDC4 protein, human; C075430:serglycin