title: Inhibition of fatty acid synthase supresses osteosarcoma cell invasion and migration.
abstract: BACKGROUND
  Fatty acid synthase (FASN) is overexpressed in a variety of human cancers, and may be involved in cancer metastasis. Hence, the strategies targeted on FASN may have therapeutic potential for treating cancer metastasis.
  
  
  OBJECTIVE
  The aim of this study is to investigate the correlation of FASN expression with metastasis in human osteosarcoma.
  
  
  METHODS
  Human osteosarcoma cell lines U2-OS and osteosarcoma biopsy specimens were employed in this study. The expression of FASN protein in osteosarcoma specimens was detected by IHC (immunohistochemistry) and the relationship with metastasis was analyzed. We performed the cerulenin, an inhibitor of FASN, to inhibit FASN expression in U2-OS cells. Western blot and RT-PCR were performed to investigate the expression of FASN in U2-OS cells. Cells mobility was detected by wound healing and Transwell assays.
  
  
  RESULTS
  Results showed that the FASN expression level in the cases with pulmonary metastases was significantly higher than in those without metastasis. In vitro, the invasion and migration of U2-OS cells were suppressed by inhibiting FASN. Our findings suggested that FASN may be involved in osteosarcoma metastasis.
authors: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Nanchang, China.
authors: Zhi Li
authors: ZL
authors: Liu
authors: Yang
authors: Y
authors: Zhou
authors: Qing Feng
authors: QF
authors: Luo
authors: MinWei
authors: M
authors: Hu
authors: Gao
authors: G
authors: Wang
authors: Shan Hu
authors: SH
authors: Huang
authors: Yong
authors: Y
authors: Shu
mesh_terms: D000293:Adolescent; D000970:Antineoplastic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002569:Cerulenin / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D004791:Enzyme Inhibitors / Q000494:pharmacology; D054890:Fatty Acid Synthase, Type I / Q000037:antagonists & inhibitors*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D012516:Osteosarcoma / Q000473:pathology* / Q000556:secondary*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D002569:Cerulenin; C518729:FASN protein, human; D054890:Fatty Acid Synthase, Type I