title: Improved neutralising antibody response against foot-and-mouth-disease virus in mice inoculated with a multi-epitope peptide vaccine using polyinosinic and poly-cytidylic acid as an adjuvant.
abstract: A peptide-based vaccine for foot-and-mouth disease (FMD) was designed. The peptide immunogen had a G-H loop domain optimised for immunogenicity and broad-spectrum antigenicity to different lineages of serotype-O FMD viruses (FMDVs). Polyinosinic and poly-cytidylic acid [poly (I:C)] was used as the adjuvant to overcome the low humoral antibody levels often observed in association with peptide-based vaccines. The multi-epitope peptide alone induced the secretion of a certain level of neutralising antibodies in mice. In contrast, co-administration of the multi-epitope peptide with poly (I:C) induced the secretion of a significantly higher level of neutralising antibodies (P<0.005). Indeed, the resultant level was slightly higher even than that induced by the inactivated vaccine (P>0.05). These initial results indicate that poly (I:C) is highly effective as an adjuvant for use with the FMDV multi-epitope peptide vaccine. This combination could yield a promising vaccine for the prevention and control of FMD. Further study is needed to evaluate the efficiency of this combination on animals susceptible naturally to FMDV.
authors: State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. caoym518@126.com
authors: Yimei
authors: Y
authors: Cao
authors: Zengjun
authors: Z
authors: Lu
authors: Pinghua
authors: P
authors: Li
authors: Pu
authors: P
authors: Sun
authors: Yuanfang
authors: Y
authors: Fu
authors: Xingwen
authors: X
authors: Bai
authors: Huifang
authors: H
authors: Bao
authors: Yingli
authors: Y
authors: Chen
authors: Dong
authors: D
authors: Li
authors: Zaixin
authors: Z
authors: Liu
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D000939:Epitopes / Q000276:immunology; D005260:Female; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011070:Poly I-C / Q000008:administration & dosage*; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000939:Epitopes; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; D011070:Poly I-C