title: Microsatellite loci in Ipomopsis aggregata (Polemoniaceae) and cross-species applicability for ecological genetics studies.
abstract: OBJECTIVE
  Novel microsatellite primers were developed for the native wildflower Ipomopsis aggregata to facilitate ongoing studies of the genetics of local adaptation and patterns of hybridization with closely related species within the genus.
  
  
  RESULTS
  Thirteen primer sets were successfully developed and tested using populations from the Rocky Mountains of Colorado, USA. The primers amplified di-, tri-, and tetranucleotide repeats with 1-15 alleles per locus. All primers also amplified fragments with varying success in closely related Ipomopsis species and more distant members of the Polemoniaceae.
  
  
  CONCLUSIONS
  The polymorphism levels observed across all loci suggest that these microsatellites may be useful for population genetic studies in Ipomopsis, as well as in studies of other related taxa in the Polemoniaceae.
authors: Department of Biology, University of Richmond, 28 Westhampton Way, Richmond, VA 23173, USA. cwu@richmond.edu
authors: Carrie A
authors: CA
authors: Wu
authors: Clifford J
authors: CJ
authors: Smith
authors: Andrew J
authors: AJ
authors: Massaro
mesh_terms: D003120:Colorado; D018744:DNA, Plant / Q000235:genetics; D005828:Genetics, Population / Q000379:methods; D006824:Hybridization, Genetic; D019684:Magnoliopsida / Q000235:genetics*; D018895:Microsatellite Repeats; D013045:Species Specificity
chemical_list: D018744:DNA, Plant