title: A new technique for obtaining mitotic chromosome spreads from fishes in the field.
abstract: This study presents an adaptation of current methodologies for preparing mitotic chromosomes from fishes, optimized for use in the field. The high-quality preparations obtained using this modified methodology is suitable for subsequent chromosomal analysis. Importantly, this method is particularly useful when specimen collection sites are far from research laboratories or when researchers are working with highly sensitive species that do not survive long outside of their natural habitats.
authors: Departamento de Genética e Evolução, Laboraório de Citogenética, Universidade Federal de São Carlos, Via Washington Luís Km 235, São Carlos, SP 13565-905, Brazil.
authors: D R
authors: DR
authors: Blanco
authors: L A C
authors: LA
authors: Bertollo
authors: R L
authors: RL
authors: Lui
authors: M R
authors: MR
authors: Vicari
authors: V P
authors: VP
authors: Margarido
authors: R F
authors: RF
authors: Artoni
authors: O
authors: O
authors: Moreira-Filho
mesh_terms: D000818:Animals; D002875:Chromosomes; D005260:Female; D005399:Fishes / Q000235:genetics*; D007621:Karyotyping / Q000379:methods*; D008297:Male; D013048:Specimen Handling / Q000379:methods*