title: Formation of lipid bilayers inside microfluidic channel array for monitoring membrane-embedded nanopores of phi29 DNA packaging nanomotor.
abstract: An efficient method to form lipid bilayers inside an array of microfluidic channels has been developed and applied to monitor the membrane-embedded phi29 DNA packaging motor with an electrochemical characterization on a lab-on-a-chip (LOC) platform. A push-pull junction capturing approach was applied to confine a small amount of the lipid solution inside a microchannel. The selective permeability between solvents and water in PDMS was utilized to extract the solvent from the lipid solution, resulting in a self-formation of the lipid bilayer in the microchannel array. Each microchannel was independently connected to a silver/silver chloride (Ag/AgCl) electrode array, leading to a high-throughput monitoring of the nanopore insertion in the formed lipid bilayers. The formation of multiple lipid bilayers inside an array of microchannels and the simultaneous electrical and optical monitoring of multiple bilayer provides an efficient LOC platform for the further development of single phi29 motor pore sensing and high throughput single pore dsDNA sequencing.
authors: College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USA.
authors: Joon S
authors: JS
authors: Shim
authors: Jia
authors: J
authors: Geng
authors: Chong H
authors: CH
authors: Ahn
authors: Peixuan
authors: P
authors: Guo
mesh_terms: D017103:Bacillus Phages / Q000737:chemistry; D015374:Biosensing Techniques; D042003:DNA Packaging; D004566:Electrodes; D004867:Equipment Design; D059014:High-Throughput Nucleotide Sequencing; D008051:Lipid Bilayers / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D058608:Nanopores / Q000648:ultrastructure*; D017422:Sequence Analysis, DNA; D018030:Silver Compounds / Q000378:metabolism; D012997:Solvents / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D018030:Silver Compounds; D012997:Solvents; D014764:Viral Proteins; D014867:Water; C037548:silver chloride