title: Transplantation of human amniotic mesenchymal stem cells in the treatment             of focal cerebral ischemia.
abstract: Cerebrovascular injury is one of the three major causes of death and is             the leading cause of adult disability. Despite the increasing progress in emergency             treatment and early rehabilitation in patients with cerebrovascular injury, treatment             options for neurological dysfunction that presents at a later stage are lacking.             This study examined the potential of human amniotic mesenchymal stem cell (hAMSC)             transplantation in the repair of neurological deficits in an experimental focal             cerebral ischemia model. Following the isolation of hAMSCs, growth characteristics             and surface antigen expression were observed. Butylated hydroxyanisole (BHA) was             used to induce the cultured cells into neuron-like cells, which were identified             by immunocytochemistry. The suture model was used to induce focal cerebral ischemia             in rats, which were subsequently randomly divided into experimental and control             groups for treatment with BrdU-labeled hAMSCs or PBS, respectively. Neurological             deficits were assessed following transplantation using the neurological severity             score, beam balance test and elevated body swing test. Eight weeks later, rat             brain tissue was analyzed with H&E staining and BrdU immunohistochemistry,             and the survival and spatial distribution of the transplanted hAMSCs were determined.             The hAMSCs proliferated in vitro, and it was found that neuron-specific enolase             (NSE) was expressed in neurons, whereas glial fibrillary acidic protein (GFAP)             was expressed in astrocytes. The focal ischemia model caused varying degrees of             left limb hemiplegia accompanied by right sided Horner's Syndrome. When examined             1, 3, 6 and 8 weeks later, significant recovery in neurological behavior was detected             in the rats treated with hAMSC transplantation compared with the control (P<0.01).             BrdU-labeled hAMSCs were concentrated near the graft site and surrounding areas,             in certain cases migrating towards the ischemic lesion. Local gliosis and lymphocytic             infiltration were not detected. hAMSCs exhibit great potential for proliferation             and are induced to differentiate into NSE-expressing neuron-like cells following             treatment with BHA. Moreover, hAMSC transplantation may improve neurological symptoms             following focal cerebral ischemia.
authors: Institute of Medical Biotechnology, Department of Human Anatomy, Histology and Embryology, School of Biology and Basic Medical Sciences, Soochow University, and Children's Hospital Affiliated to Suzhou University, Jiangsu Province Key Laboratory of Stem Cells, Suzhou 215007, PR China.
authors: Fang
authors: F
authors: Li
authors: Zong-Ning
authors: ZN
authors: Miao
authors: Yun-Yun
authors: YY
authors: Xu
authors: Shi-Ying
authors: SY
authors: Zheng
authors: Ming-De
authors: MD
authors: Qin
authors: Yan-Zheng
authors: YZ
authors: Gu
authors: Xue-Guang
authors: XG
authors: Zhang
mesh_terms: D000650:Amnion / Q000166:cytology*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002545:Brain Ischemia / Q000628:therapy*; D001973:Bromodeoxyuridine / Q000737:chemistry; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009474:Neurons / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005904:Glial Fibrillary Acidic Protein; D002083:Butylated Hydroxyanisole; D010751:Phosphopyruvate Hydratase; D001973:Bromodeoxyuridine