title: A magnetic Gram stain for bacterial detection.
abstract: Magnetizing: Bacteria are often classified into gram-positive and gram-negative strains by staining with crystal violet (CV). The described bioorthogonal modification of CV with trans-cyclooctene (TCO) can be used to render gram-positive bacteria magnetic with tetrazine-functionalized magnetic nanoparticles (MNP-Tz). This method allows class-specific automated magnetic detection and magnetic separation.
authors: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA.
authors: Ghyslain
authors: G
authors: Budin
authors: Hyun Jung
authors: HJ
authors: Chung
authors: Hakho
authors: H
authors: Lee
authors: Ralph
authors: R
authors: Weissleder
mesh_terms: D034242:Cyclooctanes / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry* / Q000166:cytology; D005840:Gentian Violet / Q000138:chemical synthesis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000166:cytology; D013237:Stereoisomerism
chemical_list: D034242:Cyclooctanes; D058185:Magnetite Nanoparticles; D005840:Gentian Violet