title: Bio-click chemistry: enzymatic functionalization of PEGylated capsules for targeting applications.
abstract: All sorted: The enzyme Sortase A was used to catalyze functionalization of PEGylated capsules with an activation-specific anti-platelet single-chain antibody (scFv). This enzymatic method allows fast, covalent, and site-directed functionalization of delivery vehicles under mild conditions. Activation-specific anti-platelet scFv-coated PEGylated capsules exhibited a high level of selective binding to thrombi, thus suggesting their potential for thrombosis therapy.
authors: Department of Chemical and Biomolecular Engineering, The University of Melbourne, Victoria 3010, Australia.
authors: Melissa K M
authors: MK
authors: Leung
authors: Christoph E
authors: CE
authors: Hagemeyer
authors: Angus P R
authors: AP
authors: Johnston
authors: Catalina
authors: C
authors: Gonzales
authors: Marloes M J
authors: MM
authors: Kamphuis
authors: Katie
authors: K
authors: Ardipradja
authors: Georgina K
authors: GK
authors: Such
authors: Karlheinz
authors: K
authors: Peter
authors: Frank
authors: F
authors: Caruso
mesh_terms: D019881:Aminoacyltransferases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002214:Capsules / Q000737:chemistry / Q000378:metabolism; D057930:Click Chemistry; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D016503:Drug Delivery Systems; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000378:metabolism; D057127:Single-Chain Antibodies / Q000737:chemistry* / Q000378:metabolism; D013211:Staphylococcus aureus / Q000201:enzymology*; D013927:Thrombosis / Q000188:drug therapy
chemical_list: D001426:Bacterial Proteins; D002214:Capsules; D004337:Drug Carriers; D057127:Single-Chain Antibodies; D011092:Polyethylene Glycols; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases