title: Investigation of the cytocidal potential of Rhinella jimi skin methanol extracts.
abstract: BACKGROUND
  Amphibian skins have wide variety of biologically active compounds associated with the natural defenses of these animals.
  
  
  OBJECTIVE
  To study the in vitro anticancer activity of methanol extracts of the skin of Rhinella jimi Stevaux (Anura: Bufonidae).
  
  
  METHODS
  The extract was obtained by cold methanol extraction for 96 h using dried skins (295 mg). The methanol skin extract was dried under reduced pressure, giving a 5.5% yield. In order to test for growth-inhibitory activity, in vitro tests were performed with the following cancer cell lines using concentrations ranging between 0.25-250 µg/mL of the extract by 48 h: K562 (leukemia), MCF-7 (breast), NCI-ADR (breast with MDR phenotype), UACC-62 (melanoma), NCI460 (lung), PCO3 (prostate), HT-29 (colon), OVCAR (ovary), and 786-0 (kidney).
  
  
  RESULTS
  The methanol extract of R. jimi produced a growth inhibition in a dose-dependent manner against the most of the assayed cell lines. In addition to the growth inhibition, the extract induced the cell death in the ovary and colon lines (EC₅₀ 0.125 and 0.2 µg/mL, respectively), demonstrating 100% of inhibition with 2.5 µg/mL. However, prostate and leukemia cell lines demonstrated less sensitivity, with EC₅₀ of 24 and 235 µg/mL, respectively. This is the first report about the anticancer activity by natural products from the skin of R. jimi.
  
  
  CONCLUSIONS
  The methanol extracts of R. jimi significantly affected the growth of several cell lines, demonstrating that these compounds are a potential source of substances that could be utilized in cancer treatments.
authors: Universidade Federal da Paraíba - UFPB, João Pessoa, PB, Brazil.
authors: S V
authors: SV
authors: Brito
authors: D L
authors: DL
authors: Sales
authors: J G M
authors: JG
authors: Costa
authors: F F G
authors: FF
authors: Rodrigues
authors: F S
authors: FS
authors: Ferreira
authors: E C
authors: EC
authors: Angélico
authors: J E
authors: JE
authors: Carvalho
authors: W O
authors: WO
authors: Almeida
authors: L A
authors: LA
authors: Anjos
authors: H D M
authors: HD
authors: Coutinho
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001938:Brazil; D002025:Bufonidae / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy; D055808:Drug Discovery; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D008297:Male; D000432:Methanol / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy*; D010051:Ovarian Neoplasms / Q000188:drug therapy; D012867:Skin / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D012997:Solvents; D014020:Tissue Extracts; D000432:Methanol