title: High-speed X-ray microscopy by use of high-resolution zone plates and synchrotron radiation.
abstract: X-ray microscopy based on synchrotron radiation has become a fundamental tool in biology and life sciences to visualize the morphology of a specimen. These studies have particular requirements in terms of radiation damage and the image exposure time, which directly determines the total acquisition speed. To monitor and improve these key parameters, we present a novel X-ray microscopy method using a high-resolution zone plate as the objective and the matching condenser. Numerical simulations based on the scalar wave field theory validate the feasibility of the method and also indicate the performance of X-ray microscopy is optimized most with sub-10-nm-resolution zone plates. The proposed method is compatible with conventional X-ray microscopy techniques, such as computed tomography, and will find wide applications in time-resolved and/or dose-sensitive studies such as living cell imaging.
authors: National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, China.
authors: Qiyue
authors: Q
authors: Hou
authors: Zhili
authors: Z
authors: Wang
authors: Kun
authors: K
authors: Gao
authors: Zhiyun
authors: Z
authors: Pan
authors: Dajiang
authors: D
authors: Wang
authors: Xin
authors: X
authors: Ge
authors: Kai
authors: K
authors: Zhang
authors: Youli
authors: Y
authors: Hong
authors: Peiping
authors: P
authors: Zhu
authors: Ziyu
authors: Z
authors: Wu
mesh_terms: D000465:Algorithms; D004867:Equipment Design; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000648:ultrastructure; D017356:Synchrotrons; D014965:X-Rays
chemical_list: D011506:Proteins