title: Rapid and simple preparation of a reagentless glucose electrochemical biosensor.
abstract: A rapid and simple procedure was developed for the preparation of a highly stable and leach-proof glucose oxidase (GOx)-bound glassy carbon electrode (GCE). Crosslinked GOx via glutaraldehyde was drop-cast on a KOH-pretreated GCE followed by drop-casting of 3-aminopropyltriethoxysilane (APTES) to form a stable bioactive layer. At -0.45 V, the biosensor exhibited a wide dynamic detection range of 0.5-48 mM for commercial glucose and 1.3-28.2 mM for Sugar-Chex blood glucose linearity standards. Several endogenous electroactive substances and drug metabolites commonly found in blood were tested and provoked no signal response. To our knowledge, the developed procedure is the most rapid method for preparing a glucose biosensor. The biosensor suffered no biofouling after 7 days of immersion in Sugar-Chex blood glucose. With excellent production reproducibility, GOx-bound electrodes stored dry at room temperature retained their initial activity after several weeks.
authors: NUSNNI-NanoCore, National University of Singapore, T-Lab Level 11, 5A Engineering Drive 1, Singapore 117580.
authors: Dan
authors: D
authors: Zheng
authors: Sandeep Kumar
authors: SK
authors: Vashist
authors: Khalid
authors: K
authors: Al-Rubeaan
authors: John H T
authors: JH
authors: Luong
authors: Fwu-Shan
authors: FS
authors: Sheu
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
chemical_list: D004800:Enzymes, Immobilized; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose