title: Selenium deficiency decreases antioxidative capacity and is detrimental to bone microarchitecture in mice.
abstract: Selenium (Se), an essential mineral, plays a major role in cellular redox status and may have beneficial effects on bone health. The objective of this study was to determine whether Se deficiency affects redox status and bone microarchitecture in a mouse model. Thirty-three male C57BL/6J mice, 18 wk old, were randomly assigned to 3 groups. Mice were fed either a purified, Se-deficient diet (SeDef) containing ∼0.9 μg Se/kg diet, or Se-adequate diets containing ∼100 μg Se/kg diet from either selenomethionine (SeMet) or pinto beans (SeBean) for 4 mo. The Se concentration, glutathione peroxidase (GPx1) activity, and GPx1 mRNA in liver were lower in the SeDef group than in the SeMet or SeBean group. The femoral trabecular bone volume/total volume and trabecular number were less, whereas trabecular separation was greater, in the SeDef group than in either the SeMet or SeBean group (P < 0.05). Bone structural parameters between the SeMet and SeBean groups did not differ. Furthermore, Serum concentrations of C-reactive protein, tartrate-resistant acid phosphatase, and intact parathyroid hormone were higher in the SeDef group than in the other 2 groups. These findings demonstrate that Se deficiency is detrimental to bone microarchitecture by increasing bone resorption, possibly through decreasing antioxidative potential.
authors: USDA, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND, USA. Jay.Cao@ars.usda.gov
authors: Jay J
authors: JJ
authors: Cao
authors: Brian R
authors: BR
authors: Gregoire
authors: Huawei
authors: H
authors: Zeng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001835:Body Weight; D015519:Bone Density / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D012643:Selenium / Q000172:deficiency*; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D005979:Glutathione Peroxidase; D012643:Selenium; D000094765:Glutathione Peroxidase GPX1; C000722068:Gpx1 protein, mouse