title: Inhibition of HIV-1 Integrase gene expression by 10-23 DNAzyme.
abstract: HIV Integrase (IN) is an enzyme that is responsible for the integration of the proviral genome into the human genome, and this integration step is the first step of the virus hijacking the human cell machinery for its propagation and replication. 10-23 DNAzyme has the potential to suppress gene expressions through sequence-specific mRNA cleavage. We  have designed three novel DNAzymes, DIN54, DIN116, and DIN152, against HIV-1 Integrase gene using Mfold software and evaluated  them for target site cleavage activity on the in vitro transcribed mRNA. All DNAzymes were tested for its inhibition of  expression of HIV Integrase protein in the transiently transfected cell lines. DIN116 and DIN152 inhibited IN-EGFP expression  by 80 percent and 70 percent respectively.
authors: Dr BR Ambedkar Center for Biomedical Research, University of Delhi, New Delhi 110 007, India.
authors: Nirpendra
authors: N
authors: Singh
authors: Atul
authors: A
authors: Ranjan
authors: Souvik
authors: S
authors: Sur
authors: Ramesh
authors: R
authors: Chandra
authors: Vibha
authors: V
authors: Tandon
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D021881:DNA, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D019427:HIV Integrase / Q000096:biosynthesis / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D059367:RNA Cleavage; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012984:Software
chemical_list: D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D012333:RNA, Messenger; C402582:RNA-cleaving DNA 10-23; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D019427:HIV Integrase; C514801:p31 integrase protein, Human immunodeficiency virus 1