title: Supramolecular polymeric chemosensor for biomedical applications: design and synthesis of a luminescent zinc metallopolymer as a chemosensor for adenine detection.
abstract: Adenine is an important bio-molecule that plays many crucial roles in food safety and biomedical diagnostics. Differentiating adenine from a mixture of adenosine and other nucleic bases (guanine, thymine, cytosine, and uracil) is particularly important for both biological and clinical applications. A neutral Zn(II) metallosupramolecular polymer based on acyl hydrazone derived coordination centres (P1) were generated through self-assembly polymerization. It is a linear coordination polymer that behaves like self-standing film. The synthesis, (1)H-NMR characterization, and spectroscopic properties of this supramolecular material are reported. P1 was found to be a chemosensor specific to adenine, with a luminescent enhancement. The binding properties of P1 with common nucleic bases and nucleosides reveal that this supramolecular polymer is very selective to adenine molecules (~20 to 420 times more selectivity than other nucleic bases). The formation constant (K) of P1 to adenine was found to be log K = 4.10 ± 0.02. This polymeric chemosensor produces a specific response to adenine down to 90 ppb. Spectrofluorimetric and (1)H-NMR titration studies showed that the P1 polymer allows each Zn(II) coordination centre to bind to two adenine molecules through hydrogen bonding with their imine and hydrazone protons.
authors: Department of Science and Environmental Studies, The Hong Kong Institute of Education, Tai Po, New Territories, HK SAR, China. cfchow@ied.edu.hk
authors: Cheuk-Fai
authors: CF
authors: Chow
mesh_terms: D000225:Adenine / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D060326:Chemistry Techniques, Synthetic; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D015195:Drug Design; D006835:Hydrazones / Q000737:chemistry; D057230:Limit of Detection; D049408:Luminescent Agents / Q000138:chemical synthesis / Q000737:chemistry; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011522:Protons; D015032:Zinc / Q000737:chemistry*
chemical_list: D006835:Hydrazones; D049408:Luminescent Agents; D009942:Organometallic Compounds; D011108:Polymers; D011522:Protons; D015032:Zinc; D000225:Adenine; D004121:Dimethyl Sulfoxide