title: Simultaneous circulation of all four dengue serotypes in Manaus, State of Amazonas, Brazil in 2011.
abstract: BACKGROUND
  Manaus, the capital city of the state of Amazon with nearly 2 million inhabitants, is located in the middle of the Amazon rain forest and has suffered dengue outbreaks since 1998.
  
  
  METHODS
  In this study, blood samples were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), aimed at identifying dengue virus serotypes.
  
  
  RESULTS
  Acute phase sera from 432 patients were tested for the presence of dengue virus. Out of the 432 patients, 137 (31.3%) were found to be positive. All the four dengue virus serotypes were observed.
  
  
  CONCLUSIONS
  The simultaneous circulation of the four dengue serotypes is described for the first time in Manaus and in Brazil.
authors: Laboratório de Virologia, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, AM, Brasil.
authors: Michele de Souza
authors: Mde S
authors: Bastos
authors: Regina Maria Pinto de
authors: RM
authors: Figueiredo
authors: Rajendranath
authors: R
authors: Ramasawmy
authors: Evaulino
authors: E
authors: Itapirema
authors: João Bosco Lima
authors: JB
authors: Gimaque
authors: Lucilaide Oliveira
authors: LO
authors: Santos
authors: Luiz Tadeu Moraes
authors: LT
authors: Figueiredo
authors: Maria Paula Gomes
authors: MP
authors: Mourão
mesh_terms: D001938:Brazil / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics*; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping
chemical_list: D012367:RNA, Viral