title: A systematic study of transfection efficiency and cytotoxicity in HeLa cells using iron oxide nanoparticles prepared with organic and inorganic bases.
abstract: Magnetic iron oxide nanoparticles (magnetite) (MNPs) were prepared using different organic and inorganic bases. Strong inorganic base (KOH) and organic bases (NH(4)OH and 1,4-diazabicyclo[2.2.2]octane (DABCO)) were used in the syntheses of the MNPs. The MNPs were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and magnetization measurements. MNPs prepared with strong inorganic base yielded an average size of 100 nm, whereas the average size of the MNPs prepared with the organic bases was 150 nm. The main competitive phase for MNPs prepared with the strong inorganic and organic bases was maghemite; however, syntheses with KOH yielded a pure magnetite phase. The transfection study performed with the MNPs revealed that the highest transfection rate was obtained with the MNPs prepared with KOH (74%). The correlation between the magnetic parameters and the transfection ratio without transfection agents indicated that MNPs prepared with KOH were a better vector for possible applications of these MNPs in biomedicine. HeLa cells incubated with MNP-KOH at 10 μg/mL for 24 and 48 h exhibited a decrease in population in comparison with the control cells and it was presumably related to the toxicity of the MNPs. However, the cells incubated with MNP-KOH at 50 and 100 μg/mL presented a very small difference in the viability between the cell populations studied at 24 and 48 h. These data illustrate the viability of HeLa cells treated with MNP-KOH and suggest the potential use of these MNPs in biomedical applications.
authors: Departament of Biology, São Paulo State University, São José do Rio Preto, São Paulo, Brazil.
authors: Marilia Freitas
authors: MF
authors: Calmon
authors: Aryane Tofanello
authors: AT
authors: de Souza
authors: Natalia Maria
authors: NM
authors: Candido
authors: Maria Irene Bartolomeu
authors: MI
authors: Raposo
authors: Sebastião
authors: S
authors: Taboga
authors: Paula
authors: P
authors: Rahal
authors: Jose G
authors: JG
authors: Nery
mesh_terms: D064753:Ammonium Hydroxide; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000494:pharmacology; D005290:Ferric Compounds / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D010879:Piperazines / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D004337:Drug Carriers; D005290:Ferric Compounds; D006878:Hydroxides; D058185:Magnetite Nanoparticles; D010879:Piperazines; D017680:Potassium Compounds; C000499:ferric oxide; D064753:Ammonium Hydroxide; C029943:potassium hydroxide; C007306:triethylenediamine