title: A need for speed: genetic encoding of rapid cycloaddition chemistries for protein labelling in living cells.
abstract: Rapid reactions: Several reactants for strain-promoted cycloaddition reactions have been genetically encoded as the side chains of noncanonical amino acids. This results in decisive improvements for the fluorescent labelling of intracellular proteins such as quantitative turnover, completion of labelling reactions within minutes, fluorogenic effects and even partial orthogonality for multicolour labelling.
authors: Fachbereich Chemie, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
authors: Moritz J
authors: MJ
authors: Schmidt
authors: Daniel
authors: D
authors: Summerer
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003500:Cyclization; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D050296:Microbial Viability; D015394:Molecular Structure; D009930:Organic Chemicals / Q000138:chemical synthesis / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000596:Amino Acids; D009930:Organic Chemicals; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins