title: Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer.
abstract: OBJECTIVE
  This study aims to evaluate the production of interferon-gamma and interleukin-10 by stimulated peripheral blood mononuclear cells isolated from patients with supraglottic laryngeal cancer before and after surgical treatment.
  
  
  METHODS
  Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cultures of peripheral blood mononuclear cells isolated during the preoperative and late postoperative periods were stimulated with concanavalin A and Bacille Calmette-Guerin, and the supernatant concentrations of interferon-gamma and interleukin-10 were measured.
  
  
  RESULTS
  For non-stimulated cultures, the interferon-gamma levels produced by the preoperative period and the late postoperative period cultures were lower than the levels produced by the control group cultures. The interferon-gamma levels after stimulation with concanavalin A were higher in the late postoperative period cultures than in the preoperative evaluation cultures. Stimulation with Bacille Calmette-Guerin led to the production of similar levels of interferon-gamma and interleukin-10 by all cultures; thus, stimulation increased the levels of interferon-gamma produced by both the preoperative and postoperative cultures relative to the levels produced by the corresponding unstimulated cultures.
  
  
  CONCLUSIONS
  Patients with advanced supraglottic laryngeal cancer exhibit an in vitro deficiency in interferongamma secretion by mononuclear cells. Stimulated cells seem to recover this function during the postoperative period.
authors: Medical School of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. lcconti@fmrp.usp.br
authors: Luiz C
authors: LC
authors: Conti-Freitas
authors: Maria C
authors: MC
authors: Foss-Freitas
authors: Rui C M
authors: RC
authors: Mamede
authors: Norma T
authors: NT
authors: Foss
mesh_terms: D000293:Adolescent; D000328:Adult; D002277:Carcinoma / Q000097:blood* / Q000473:pathology; D016022:Case-Control Studies; D002648:Child; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000097:blood; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000097:blood; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000097:blood; D007822:Laryngeal Neoplasms / Q000097:blood* / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D009163:Mycobacterium bovis; D009367:Neoplasm Staging; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D016207:Cytokines; D008934:Mitogens; D003208:Concanavalin A; D016753:Interleukin-10; D007371:Interferon-gamma