title: Trabecular meshwork length in men and women by histological assessment.
abstract: OBJECTIVE
  To evaluate the length of the trabecular meshwork (TM) from the scleral spur (SS) to Schwalbe's line (SL) and assess the detectability of the SS in histopathology specimens.
  
  
  METHODS
  This study included 158 angle images from 79 cross-sectional slides derived from eyes enucleated for melanoma. The slides were stained with hematoxylin-eosin (HE) or periodic acid schiff (PAS). Two ophthalmologists evaluated the TM length by using the slides stained with HE to assess the interobserver reproducibility. For intraobserver reproducibility, the first observer assessed 79 images in a different session. Also, 30 images that were randomly selected for PAS stain were evaluated to assess the agreement of the measurements between HE and PAS staining. Interclass correlation coefficients (ICC) were calculated to evaluate reproducibility of measurements. The images were also evaluated for detectability of the scleral spurs.
  
  
  RESULTS
  Among the 79 included subjects, 40 were male and 39 were female. The average trabecular meshwork length was 694.9 ± 109.0 µm in the male group and 713.2 ± 106.9 µm in the female group (p = 0.29). Intraobserver and interobserver ICC were 0.89 and 0.62, respectively. ICC for agreement between HE and PAS was 0.89. Among the 158 angles graded, the first observer graded 40 images (25.3%) and the second observer graded 45 (28.5%) as difficult to identify the scleral spur.
  
  
  CONCLUSIONS
  There was no statistically significant difference between the average trabecular meshwork length in men and women. Among the angles evaluated, 25.3-28.5% were graded as difficult to identify the scleral spur.
authors: Department of Ophthalmology, University of California, San Francisco, CA, USA.
authors: Toshimitsu
authors: T
authors: Kasuga
authors: Yi-Chun
authors: YC
authors: Chen
authors: Michele M
authors: MM
authors: Bloomer
authors: Kristin E
authors: KE
authors: Hirabayashi
authors: Yoshimune
authors: Y
authors: Hiratsuka
authors: Akira
authors: A
authors: Murakami
authors: Shan C
authors: SC
authors: Lin
mesh_terms: D000867:Anterior Chamber / Q000473:pathology; D003430:Cross-Sectional Studies; D015353:Eye Enucleation; D005134:Eye Neoplasms / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000473:pathology / Q000601:surgery; D008875:Middle Aged; D012372:ROC Curve; D012189:Retrospective Studies; D012737:Sex Factors; D041623:Tomography, Optical Coherence / Q000379:methods*; D014129:Trabecular Meshwork / Q000473:pathology*