title: Comparative analyses of H3K4 and H3K27 trimethylations between the mouse cerebrum and testis.
abstract: The global features of H3K4 and H3K27 trimethylations (H3K4me3 and H3K27me3) have been well studied in recent years, but most of these studies were performed in mammalian cell lines. In this work, we generated the genome-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChIP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLiD technology. We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression, function and development. Moreover, we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes, which contradicts the findings in previous studies. Finally, we observed that bivalent domains, with both H3K4me3 and H3K27me3, existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes. However, the bivalent domains tend towards a "winner-takes-all" approach to regulate the expression of associated genes. We also verified the above results in mouse ES cells. As expected, the results in ES cells are consistent with those in cerebrum and testis. In conclusion, we present two very important findings. One is that H3K4me3 and H3K27me3 rarely occur in silent genes. The other is that bivalent domains may adopt a "winner-takes-all" principle to regulate gene expression.
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, China.
authors: Peng
authors: P
authors: Cui
authors: Wanfei
authors: W
authors: Liu
authors: Yuhui
authors: Y
authors: Zhao
authors: Qiang
authors: Q
authors: Lin
authors: Daoyong
authors: D
authors: Zhang
authors: Feng
authors: F
authors: Ding
authors: Chengqi
authors: C
authors: Xin
authors: Zhang
authors: Z
authors: Zhang
authors: Shuhui
authors: S
authors: Song
authors: Fanglin
authors: F
authors: Sun
authors: Jun
authors: J
authors: Yu
authors: Songnian
authors: S
authors: Hu
mesh_terms: D000818:Animals; D002460:Cell Line; D054022:Cerebrum / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000378:metabolism; D015870:Gene Expression; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008745:Methylation; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D013737:Testis / Q000378:metabolism*
chemical_list: D006657:Histones