title: Depth-resolved cellular microrheology using HiLo microscopy.
abstract: It is increasingly important to measure cell mechanical properties in three-dimensional environments. Particle tracking microrheology (PTM) can measure cellular viscoelastic properties; however, out-of-plane data can introduce artifacts into these measurements. We developed a technique that employs HiLo microscopy to reduce out-of-plane contributions. This method eliminated signals from 90% of probes 0.5 μm or further from the focal plane, while retaining all in-plane probes. We used this technique to characterize live-cell bilayers and found that there were significant, frequency-dependent changes to the extracted cell moduli when compared to conventional analysis. Our results indicate that removal of out-of-plane information is vital for accurate assessments of cell mechanical properties.
authors: Jarett
authors: J
authors: Michaelson
authors: Heejin
authors: H
authors: Choi
authors: Peter
authors: P
authors: So
authors: Hayden
authors: H
authors: Huang