title: Development and characterization of microsatellite markers for Actaea racemosa (black cohosh, Ranunculaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed in Actaea racemosa to analyze population genetic structure, compare genetic diversity across the species' range, and provide a genetic context for studies of phytochemical variation.
  
  
  RESULTS
  A total of seven polymorphic loci were screened in 60 individuals from 12 localities. The number of alleles per locus ranged from three to six, and observed heterozygosity ranged from 0.133 to 0.900. Most of the loci tested cross-amplified in A. pachypoda, A. podocarpa, and A. rubra, indicating the utility of these markers for the genus.
  
  
  CONCLUSIONS
  These new loci will provide tools for population genetics studies, including the characterization of genetic variation in A. racemosa and other eastern North American species of Actaea.
authors: Department of Biology, 132 Natural Science Building, Western Carolina University, Cullowhee, North Carolina 28723, USA.
authors: Sarah J
authors: SJ
authors: Pate
authors: Jason A
authors: JA
authors: Clement
authors: Joe-Ann H
authors: JA
authors: McCoy
authors: Stacey L
authors: SL
authors: Lance
authors: Katherine G
authors: KG
authors: Mathews
mesh_terms: D000483:Alleles; D031885:Cimicifuga / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population / Q000379:methods; D018895:Microsatellite Repeats; D010946:Plants, Medicinal / Q000235:genetics
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers