title: Confocal Raman microspectroscopy for skin characterization: a comparative study between human skin and pig skin.
abstract: The present paper provides a spectral comparison between abdominal human skin (Transkin) and pig ear skin using confocal Raman microspectroscopy at 660 nm. Pig ear skin is usually utilized as a substitute for human skin for active ingredients assessment in dermatological and cosmetics fields. Herein, the comparison is made at the level of the stratum corneum (SC), the SC/epidermis junction and the viable epidermis. The 660 nm excitation source appears to be the most appropriate wavelength for such skin characterization. From Raman signatures of both skin types, a tentative assignment of vibrations was performed in the fingerprint and the high wavenumber spectral regions. Significant differences were highlighted for lipid content in in-depth spectra and for hyaluronic acid (HA) and carotenoid in SC spectra. Marked tissular variability was also revealed by certain Raman vibrations. These intrinsic molecular data probed by confocal Raman microspectroscopy have to be considered for further applications such as cutaneous drug permeation.
authors: MéDIAN Unit, CNRS UMR 6237, Faculty of Pharmacy, University of Reims Champagne - Ardenne (URCA), 51 rue Cognacq Jay, 51096 Reims, France.
authors: Sana
authors: S
authors: Tfaili
authors: Cyril
authors: C
authors: Gobinet
authors: Gwendal
authors: G
authors: Josse
authors: Jean-François
authors: JF
authors: Angiboust
authors: Michel
authors: M
authors: Manfait
authors: Olivier
authors: O
authors: Piot
mesh_terms: D000005:Abdomen; D000818:Animals; D004817:Epidermis / Q000737:chemistry; D005260:Female; D006801:Humans; D012867:Skin / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013552:Swine