title: Biological activities of ethyl acetate extract of different parts of Hyssopus angustifolius.
abstract: BACKGROUND
  Hyssopus angustifolius M. Bieb. (Lamiaceae) is one of the most important medicinal plants in Iranian traditional medicine for the treatment of lung inflammation, laryngitis and cough relief. Much attention has been paid to this medicinal plant because of its traditional uses.
  
  
  OBJECTIVE
  The present study examined the antioxidant and antihemolytic activities of ethyl acetate extract of stems, leaf and flowers of Hyssopus angustifolius.
  
  
  METHODS
  Antioxidant activity of extracts was evaluated by employing six different models, i.e., DPPH, nitric oxide and hydrogen peroxide scavenging, metal chelating and reducing power activities and hemoglobin-induced linoleic acid system. Also, antihemolytic activity was evaluated against hydrogen peroxide-induced hemolysis.
  
  
  RESULTS
  Flowers extract showed the better activity than leaf and stems extracts in DPPH radical scavenging activity (IC₅₀ was 275.4 ± 7.6 μg mL⁻¹). Leaf, stems and flowers extracts showed good nitric oxide scavenging activity (IC₅₀ were 376.6 ± 11.4 µg mL⁻¹ for flowers, 297.6 ± 9.6 μg mL⁻¹ µg mL⁻¹ for leaves and 837.8 ± 19.2 µg mL⁻¹ for stems). The leaf extract exhibited better hydrogen peroxide scavenging and Fe²⁺ chelating activity than stems and flowers extracts. In hemoglobin-induced linoleic acid system, all of the extracts exhibited very good activity. Also, extracts show weak reducing power activity. The ethyl acetate extract of leaf showed better antihemolytic activity than the flower and stems (IC₅₀ was 94.0 ± 2.4 μg mL⁻¹).
  
  
  CONCLUSIONS
  These findings give a scientific basis to the traditional usage of Hyssopus angustifolius, also showing its potential as rich sources of natural antioxidant compounds.
authors: Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran. Nabavi208@gmail.com
authors: Heshmatollah
authors: H
authors: Alinezhad
authors: Robabeh
authors: R
authors: Baharfar
authors: Mahboobeh
authors: M
authors: Zare
authors: Razieh
authors: R
authors: Azimi
authors: Seyed Fazel
authors: SF
authors: Nabavi
authors: Seyed Mohammad
authors: SM
authors: Nabavi
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D055808:Drug Discovery; D004912:Erythrocytes / Q000187:drug effects; D020834:Ethnopharmacology; D035264:Flowers / Q000737:chemistry; D006401:Hematologic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D007492:Iran; D007502:Iron Chelating Agents / Q000302:isolation & purification / Q000494:pharmacology; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D012997:Solvents / Q000737:chemistry
chemical_list: D000085:Acetates; D000975:Antioxidants; D006401:Hematologic Agents; D007502:Iron Chelating Agents; D010936:Plant Extracts; D012997:Solvents; D009569:Nitric Oxide; C007650:ethyl acetate