title: Influence of various treatments including povidone-iodine and healing stimulatory reagents in a rabbit ear wound model.
abstract: Selecting an appropriate treatment for a given case of skin wound is crucial for inducing optimal healing. We used an animal model developed from normal rabbit ears in order to assess the efficacy of treatments for skin wounds with or without a wet dressing, anti microbial reagent or topical wound-stimulatory reagents. The degree of healing in each group was evaluated and compared using four histological parameters: (i) degree of reepithelialisation, (ii) amount of granulation tissue formation, and (iii) the number of capillary lumens and (iv) fibroblasts in the granulation tissue. Treatment using wet dressings resulted in an increase in capillary number compared with the open dry wound. Although the retention of povidone-iodine (PI) in wound tissue after application significantly inhibited reepithelialisation (P < 0.05), rinsing PI off with saline was comparable in effect to using only a wet dressing. The three topical reagents, namely, basic fibroblast growth factor, prostaglandin E1 and dibutyryl cyclic adenosine monophosphate, significantly improved reepithelialisation (P < 0.05). In conclusion, wounds should be kept hydrated by applying topical reagents. If there are any signs of bacterial infection, PI can be applied and rinsed later with saline in order to minimise its cytotoxic effects.
authors: Department of Dermatology, Tokyo Medical University, Shinjuku-ku, Tokyo, Japan Department of Dermatology, Self Defense Force Hanshin Hospital, Kawanishi, Hyogo, Japan.
authors: Keitaro
authors: K
authors: Arai
authors: Masashi
authors: M
authors: Yamazaki
authors: Tatsuo
authors: T
authors: Maeda
authors: Takaaki
authors: T
authors: Okura
authors: Ryoji
authors: R
authors: Tsuboi
mesh_terms: D000527:Alprostadil / Q000008:administration & dosage*; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage; D003994:Bucladesine / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D004423:Ear / Q000293:injuries*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D006097:Granulation Tissue / Q000187:drug effects; D011206:Povidone-Iodine / Q000008:administration & dosage*; D011817:Rabbits; D012867:Skin / Q000187:drug effects / Q000473:pathology; D014665:Vasodilator Agents / Q000008:administration & dosage; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy*
chemical_list: D000891:Anti-Infective Agents, Local; D014665:Vasodilator Agents; D016222:Fibroblast Growth Factor 2; D003994:Bucladesine; D011206:Povidone-Iodine; D000527:Alprostadil