title: Converting a solvatochromic fluorophore into a protein-based pH indicator for extreme acidity.
abstract: Live-cell pH measurements: An environment-sensitive fluorophore (green) was site-specifically introduced on HdeA, an acid-resistant chaperone showing pH-mediated conformational changes under low pH conditions. A survey of the attachment sites led to the discovery of one position on HdeA at which the attached fluorophore showed a strong fluorescence increase upon acidification.
authors: Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
authors: Maiyun
authors: M
authors: Yang
authors: Yanqun
authors: Y
authors: Song
authors: Meng
authors: M
authors: Zhang
authors: Shixian
authors: S
authors: Lin
authors: Ziyang
authors: Z
authors: Hao
authors: Yuan
authors: Y
authors: Liang
authors: Dianmu
authors: D
authors: Zhang
authors: Peng R
authors: PR
authors: Chen
mesh_terms: D000143:Acids / Q000032:analysis*; D000818:Animals; D002460:Cell Line; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry*; D051379:Mice; D008958:Models, Molecular; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D000143:Acids; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D011506:Proteins; D012997:Solvents