title: Expression of the ginseng PgPR10-1 in Arabidopsis confers resistance against fungal and bacterial infection.
abstract: Korean ginseng (Panax ginseng C. A. Meyer) consists of nine cultivars from three Jakyung, Chungkyung, and Hwangsook lines. Among three previously identified PR-10 homologs from ginseng (PgPR10-1, PgPR10-2, and PgPR10-3), we found that the exact same sequence of PgPR10-2 exist in all tested nine cultivars. But a deletion and SNP was found in American ginseng (Panax quinquefolius). PR-10 proteins are known to be small and cytosolic, and showed similar three-dimensional structure. Here we show that the heterologous overexpression of PgPR10-1 in Arabidopsis showed enhanced resistance against Pseudomonas syringe, Fusarium oxysporum, and Botrytis cinerea and in-frame tagging with fluorescent protein showed its cytoplasm and nucleus localization. Protein-protein interaction of PgPR10-2 with PgPR10-1, PgPR10-2 and PgPR10-3 suggests that the PgPR10 proteins might form multimeric complexes in different cellular compartments to function in development and in defense-related mechanism. Differential response of PgPR10-1 and PgPR10-2 against different sets of biotic stresses in ginseng plant supports this notion.
authors: Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University, Suwon 449-701, South Korea. mpizlee@hotmail.com
authors: Ok Ran
authors: OR
authors: Lee
authors: Yu-Jin
authors: YJ
authors: Kim
authors: Sri Renuka Devi
authors: SR
authors: Balusamy
authors: Altanzul
authors: A
authors: Khorolragchaa
authors: Gayathri
authors: G
authors: Sathiyaraj
authors: Min-Kyeoung
authors: MK
authors: Kim
authors: Deok-Chun
authors: DC
authors: Yang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism* / Q000382:microbiology; D001483:Base Sequence; D020171:Botrytis / Q000472:pathogenicity; D017124:Conserved Sequence; D018744:DNA, Plant / Q000235:genetics; D005670:Fusarium / Q000472:pathogenicity; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D005894:Panax / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000472:pathogenicity; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D011994:Recombinant Proteins