title: Colloidal centrifugation of stallion semen results in a reduced rate of sperm DNA fragmentation.
abstract: Stallion spermatozoa recovered and examined immediately after colloidal centrifugation resulted in a higher straight-line velocity (VSL) than sperm processed using direct conventional centrifugation (p = 0.000), but there was no differences in the progressive motility or sperm DNA fragmentation (SDF) as determined by the sperm chromatin dispersion assay. However, when centrifuged spermatozoa were incubated at 37 °C for 24 h to determine the rate of SDF (r-SDF), a lower r-SDF (p = 0.0011) was observed in those sperm recovered after colloidal separation (0.5 ± 0.1%/h) compared to direct (1.2 ± 0.4%/h) or no centrifugation (r-SDF = 1.2 ± 0.3%/h). These results confirm that colloidal separation of stallion spermatozoa results in prolonged sperm DNA longevity, but these differences were only apparent following a period of incubation and dynamic assessment. Consequently, we strongly recommend the use of the dynamic form of the SDF assay for evaluating centrifugation and/or other ex vivo procedures, as a single basal assessment of SDF may inadvertently result in a false-positive evaluation of DNA quality.
authors: Department of Reproduction, Centro Militar de CríaCaballar, FESCCR-Ministry of Defense, Ávila, Spain.
authors: F
authors: F
authors: Crespo
authors: J
authors: J
authors: Gosalvez
authors: L
authors: L
authors: Gutiérrez-Cepeda
authors: C
authors: C
authors: Serres
authors: S D
authors: SD
authors: Johnston
mesh_terms: D000818:Animals; D002498:Centrifugation / Q000379:methods / Q000662:veterinary*; D003102:Colloids; D053938:DNA Fragmentation; D006736:Horses / Q000502:physiology*; D008297:Male; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D003102:Colloids