title: Synovium-derived mesenchymal stem cells encapsulated in a novel injectable gel can repair osteochondral defects in a rabbit model.
abstract: We developed a novel injectable type I collagen/hyaluronic acid/fibrinogen (COL/HA/FG) composite gel that encapsulated synovium-derived mesenchymal stem cells (SDSCs) for the repair of damaged articular cartilage. We first analyzed the suitability of the composite gel as a three-dimensional injectable cell carrier in vitro. In an in vivo rabbit model, the COL/HA/FG composite gel displayed the potential to regenerate and repair osteochondral defects in the knee. Culture of the SDSCs encapsulated COL/HA/FG composite gel in a chondrogenic medium resulted in high viability of the SDSCs and high expressions of type II collagen, aggrecan, and sox 9 mRNA. Moreover, glycosaminoglycans and type II collagen were accumulated within the extracellular matrix. In the animal model, the SDSCs encapsulated COL/HA/FG composite gel produced a hyaline-like cartilage construct. Twenty-four weeks after transplantation, the defects had been repaired with hyaline cartilage-like tissue that was densely stained by safranin-O and immunostained by a type II collagen antibody. This data suggest that the SDSC-encapsulated COL/HA/FG composite gel can be a good therapeutic candidate/strategy for repairing of damaged articular cartilage.
authors: Department of Orthopedic Surgery, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors: Jae-Chul
authors: JC
authors: Lee
authors: Sang Young
authors: SY
authors: Lee
authors: Hyun Jin
authors: HJ
authors: Min
authors: Sun Ae
authors: SA
authors: Han
authors: Jak
authors: J
authors: Jang
authors: Sahnghoon
authors: S
authors: Lee
authors: Sang Cheol
authors: SC
authors: Seong
authors: Myung Chul
authors: MC
authors: Lee
mesh_terms: D053674:Aggrecans / Q000235:genetics; D000818:Animals; D024043:Collagen Type II / Q000235:genetics; D005782:Gels / Q000737:chemistry*; D007719:Knee Joint / Q000166:cytology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D011817:Rabbits; D055758:SOX9 Transcription Factor / Q000235:genetics; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D053674:Aggrecans; D024043:Collagen Type II; D005782:Gels; D055758:SOX9 Transcription Factor