title: [Research of mechanism of secondary metabolites of phenolic acids in Salvia miltiorrhiza hairy root induced by jasmonate].
abstract: OBJECTIVE
  To study the mechanism of secondary metabolites of some phenolic acids in the hairy roots of Salvia miltiorrhiza induced by methyl jasmonate.
  
  
  METHODS
  The hairy roots of S. miltiorrhiza were induced with methyl jasmonate (100 micromol x L(-1)) and collected at 0, 12, 24, 36 h after treatment. Real-time quantitative PCR was used for detecting the mRNA expression level of the key enzyme genes on the secondary metabolites pathway of rosmarinic acid, while a LC-MS method was developed to determine the content of rosmarinic acid, caffeic acid and salvianolic acid B.
  
  
  CONCLUSIONS
  The concentration of phenolic acids grew up and accumulated quickly in the hairy roots with exogenous signal molecule MJ induced, and it was showed that the content of CA and RA reached the maximum after 24 h and the content of LAB reached the maximum in 36 h by MJ induced. The induction mechanism may be activated with different levels of RA synthesis in PAL, 4CL, C4H genes on the key enzyme phenylalanine pathway and TAT, HPPR genes on tyrosine pathway. The time of gene expression was different, among them, 4CL and PAL genes were more important. In a word, the result can provide some basis data about the mechanism of secondary metabolites of phenolic acids for further research.
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors: Wenyuan
authors: W
authors: Li
authors: Wei
authors: W
authors: Gao
authors: Jing
authors: J
authors: Zhao
authors: Guanghong
authors: G
authors: Cui
authors: Aijuan
authors: A
authors: Shao
authors: Luqi
authors: L
authors: Huang
mesh_terms: D003517:Cyclopentanes / Q000032:analysis / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D062385:Hydroxybenzoates / Q000032:analysis / Q000378:metabolism*; D054883:Oxylipins / Q000032:analysis / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D027561:Salvia miltiorrhiza / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D062385:Hydroxybenzoates; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; C017616:phenolic acid