title: Regional astrocyte allocation regulates CNS synaptogenesis and repair.
abstract: Astrocytes, the most abundant cell population in the central nervous system (CNS), are essential for normal neurological function. We show that astrocytes are allocated to spatial domains in mouse spinal cord and brain in accordance with their embryonic sites of origin in the ventricular zone. These domains remain stable throughout life without evidence of secondary tangential migration, even after acute CNS injury. Domain-specific depletion of astrocytes in ventral spinal cord resulted in abnormal motor neuron synaptogenesis, which was not rescued by immigration of astrocytes from adjoining regions. Our findings demonstrate that region-restricted astrocyte allocation is a general CNS phenomenon and reveal intrinsic limitations of the astroglial response to injury.
authors: Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA 94143, USA.
authors: Hui-Hsin
authors: HH
authors: Tsai
authors: Huiliang
authors: H
authors: Li
authors: Luis C
authors: LC
authors: Fuentealba
authors: Anna V
authors: AV
authors: Molofsky
authors: Raquel
authors: R
authors: Taveira-Marques
authors: Helin
authors: H
authors: Zhuang
authors: April
authors: A
authors: Tenney
authors: Alice T
authors: AT
authors: Murnen
authors: Stephen P J
authors: SP
authors: Fancy
authors: Florian
authors: F
authors: Merkle
authors: Nicoletta
authors: N
authors: Kessaris
authors: Arturo
authors: A
authors: Alvarez-Buylla
authors: William D
authors: WD
authors: Richardson
authors: David H
authors: DH
authors: Rowitch
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D001921:Brain / Q000002:abnormalities / Q000166:cytology* / Q000502:physiology; D001930:Brain Injuries / Q000503:physiopathology; D002465:Cell Movement; D049452:Green Fluorescent Proteins; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins / Q000378:metabolism; D019426:Integrases / Q000235:genetics; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009046:Motor Neurons / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics; D011506:Proteins / Q000378:metabolism; D022661:RNA, Untranslated; D013116:Spinal Cord / Q000002:abnormalities / Q000166:cytology* / Q000502:physiology; D013119:Spinal Cord Injuries / Q000503:physiopathology; D013569:Synapses / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D029961:Zebrafish Proteins
chemical_list: D001426:Bacterial Proteins; D051792:Basic Helix-Loop-Helix Proteins; D049452:Green Fluorescent Proteins; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D019426:Integrases; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D022661:RNA, Untranslated; D014157:Transcription Factors; D029961:Zebrafish Proteins; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; C407737:Neurog3 protein, mouse; C054562:yellow fluorescent protein, Bacteria; C045073:Cre recombinase