title: Identification and characterization of two functionally unknown genes involved in butanol tolerance of Clostridium acetobutylicum.
abstract: Solvents toxicity is a major limiting factor hampering the cost-effective biotechnological production of chemicals. In Clostridium acetobutylicum, a functionally unknown protein (encoded by SMB_G1518) with a hypothetical alcohol interacting domain was identified. Disruption of SMB_G1518 and/or its downstream gene SMB_G1519 resulted in increased butanol tolerance, while overexpression of SMB_G1518-1519 decreased butanol tolerance. In addition, SMB_G1518-1519 also influences the production of pyruvate:ferredoxin oxidoreductase (PFOR) and flagellar protein hag, the maintenance of cell motility. We conclude that the system of SMB_G1518-1519 protein plays a role in the butanol sensitivity/tolerance phenotype of C. acetobutylicum, and can be considered as potential targets for engineering alcohol tolerance.
authors: Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors: Kaizhi
authors: K
authors: Jia
authors: Yanping
authors: Y
authors: Zhang
authors: Yin
authors: Y
authors: Li
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects* / Q000235:genetics*; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D000440:Butanols / Q000633:toxicity*; D046969:Clostridium acetobutylicum / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D005285:Fermentation / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics*; D009068:Movement / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D000440:Butanols