title: Genomic and EST-derived microsatellite markers for Iris laevigata (Iridaceae) and other congeneric species.
abstract: OBJECTIVE
  The aims of this study are to develop and characterize genomic and expressed sequence tag (EST)-derived microsatellites from Iris laevigata and test their transferability in I. ensata, I. setosa, I. halophila, I. scariosa, I. potaninii, I. tenuifolia, I. bloudowii, and I. sanguinea.
  
  
  RESULTS
  Ten genomic and six EST-derived microsatellites were characterized in I. laevigata. These microsatellite primers amplified one to five alleles in I. laevigata and some of these primers were also successfully amplified in congeneric species.
  
  
  CONCLUSIONS
  These microsatellite primers provide us an initial set of molecular markers to explore the spatial population genetic structure of I. laevigata. In addition, these markers may also be useful in population and conservation genetic studies of closely related species.
authors: Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, People's Republic of China.
authors: Ming-Zhou
authors: MZ
authors: Sun
authors: Ming-Rui
authors: MR
authors: Li
authors: Feng-Xue
authors: FX
authors: Shi
authors: Lin
authors: L
authors: Li
authors: Ying
authors: Y
authors: Liu
authors: Lin-Feng
authors: LF
authors: Li
authors: Hong-Xing
authors: HX
authors: Xiao
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D018745:Genome, Plant; D031323:Iris Plant / Q000235:genetics*; D018895:Microsatellite Repeats; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers