title: Molecular allergens in the diagnosis of latex allergy.
abstract: BACKGROUND
  Molecular allergens enable the definition of sensitization profiles in allergic patients.
  
  
  OBJECTIVE
  To validate the most helpful allergens for the diagnosis of latex allergy in different clinical situations.
  
  
  METHODS
  130 patients suspected to be allergic to latex with positive IgE against natural rubber latex (NRL) have been studied: 97 were confirmed as latex allergic (among which 55 professionally exposed to latex and 35 with a peranaesthetic anaphylactic shock) and 33 were only sensitized to latex without clinical allergy. Each serum was tested for IgE against 9 recombinant latex allergens and bromelain using Phadia ImmunoCAP 250.
  
  
  RESULTS
  rHev b 6.01, 6.02, 2 and 5 were the major allergens in the allergic population. An excellent correlation (94%) was observed between IgE against rHev b 6.01 and latex prick test positivities. IgE against rHev b 1, 3 and 5 were more frequent and their levels significantly higher in patients with peranaesthetic anaphylactic shock. Among the asymptomatic patients (29/33 allergic to pollen), NRL IgE positivity is explained by the presence of anti-rHev b 8 and/or anti-carbohydrate IgE.
  
  
  CONCLUSIONS
  rHev b 6.01 and rHev b 5 specific IgE are of major interest to confirm latex allergy diagnosis. rHev b 5 is particularly useful in case of monosensitization where clinical symptoms and latex skin prick tests may be discordant, rHev b1 and rHev b 3 are interesting to document multi-operated and peranaesthetic latex allergy. Finally, rHev b 8 is a helpful marker to highlight latex/pollen cross-reactivity which improves the specificity of the serological tests.
authors: Allergy Unit, Laboratory of Immunology, Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, Pierre Bénite F-69495, France.
authors: L
authors: L
authors: Garnier
authors: L
authors: L
authors: Selman
authors: P
authors: P
authors: Rouzaire
authors: M
authors: M
authors: Bouvier
authors: O
authors: O
authors: Roberts
authors: F
authors: F
authors: Bérard
authors: J
authors: J
authors: Bienvenu
authors: F
authors: F
authors: Bienvenu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology; D052179:Antigens, Plant / Q000276:immunology; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007840:Latex / Q000737:chemistry* / Q000276:immunology; D020315:Latex Hypersensitivity / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012882:Skin Tests; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D007840:Latex; D007073:Immunoglobulin E