title: Fluorescence detection of adenosine triphosphate using smart probe.
abstract: A novel fluorescent probe for adenosine triphosphate (ATP) assay based on DNA ligation is proposed in this article. This approach uses a novel smart probe, T4 DNA ligase, and two short oligonucleotides. In the presence of ATP, the T4 DNA ligase catalyzes the ligation reaction and the ligation product restores the fluorescence of the smart probe. This method is very sensitive with a 0.5-nM limit of detection. Compared with current assay methods, the strategy is simpler, cheaper, and 40 times more sensitive.
authors: School of Biological Science and Technology, Central South University, Changsha 410013, China. lhlmcb@yahoo.com.cn
authors: Changbei
authors: C
authors: Ma
authors: Hanchun
authors: H
authors: Chen
authors: Rui
authors: R
authors: Han
authors: Hailun
authors: H
authors: He
authors: Weimin
authors: W
authors: Zeng
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry; D001483:Base Sequence; D055162:Biocatalysis; D011088:DNA Ligases / Q000378:metabolism; D015345:Oligonucleotide Probes / Q000737:chemistry* / Q000235:genetics; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D015345:Oligonucleotide Probes; D000255:Adenosine Triphosphate; D011088:DNA Ligases