title: [The performance of the automated immune chemiluminescent system "IMMULITE 2000XPi for the measurement of serum soluble IL-2 receptor in clinical samples].
abstract: The performance of a chemical luminescence test reagent "Immulyze IL-2R II" with an automated immune chemiluminescent system "IMMULITE 2000XPi" for the measurement of serum soluble IL-2 receptor in clinical samples was investigated. The satisfactory results were obtained for the reproducibility, precision, linearity, and sensitivity, and no interference with hemolysis, bilirubin, chyle or intrafat was observed. A significant correlation was found between the values of sIL-2R measured by the Cell-free N IL-2R and those obtained by the IMMULYZE IL-2R II. The measurements were stable regardless of the methods of sample preservation, or repeated freeze-thawing procedures. Elevated concentrations of sIL-2R over 1,000 U/mL were found in multiple types of collagen diseases or severe cases of allergic diseases, indicative that sIL-2R levels might correlate with the severity of autoimmune diseases. In patients with lymphoma, sIL-2R levels correlated with the lactate dehydrogenase (LD) activity. Among the lymphoma cases with sIL-2R levels over 1,000 U/mL, the majority (84%) had significantly higher levels of LD, and among them, 81% were at the clinical stage IV. We observed that sIL-2R levels increased from the early stages of lymphoma, while LD activities increased at the advanced stages. Our present findings suggest that sIL-2R is a promising marker for the diagnosis of autoimmune and allergic diseases, and also for the diagnosis and staging of lymphomas.
authors: Department of Clinical Laboratory, Tokyo University Hospital, Bunkyo-ku, Tokyo 113-8654, Japan. WATANABEN-BLO@h.u-tokyo.ac.jp
authors: Naomi
authors: N
authors: Watanabe
authors: Ritsuko
authors: R
authors: Nagatomo
authors: Shigeo
authors: S
authors: Okubo
authors: Hiromitsu
authors: H
authors: Yokota
authors: Akiko
authors: A
authors: Masuda
authors: Hitoshi
authors: H
authors: Ikeda
authors: Yutaka
authors: Y
authors: Yatomi
mesh_terms: D001327:Autoimmune Diseases / Q000097:blood* / Q000276:immunology; D057205:Automation, Laboratory / Q000379:methods*; D014408:Biomarkers, Tumor / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D008228:Lymphoma, Non-Hodgkin / Q000097:blood* / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000097:blood* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility
chemical_list: D014408:Biomarkers, Tumor; D015375:Receptors, Interleukin-2