title: Quaternized celluloses as new dynamic coatings in capillary electrophoresis for basic protein separation.
abstract: In this work, a series of quaternized celluloses (QCs), homogeneously synthesized in the NaOH/urea aqueous solutions, were studied as dynamic coatings for capillary electrophoresis. Capillaries coated with these cationic cellulose derivatives at the concentration as low as 3 μg/mL were able to generate a stable, reversed electroosmotic flow. The effects of QC molecular parameters, such as the degree of cationic substitution and molecular weight, and the effect of buffer pH on the EOF mobility as well as the separation of basic proteins were investigated in detail. It was shown that the use of QC coatings in CE could drastically reduce the analysis time and improve the separation performance within a broad pH range. Five basic proteins, that is, lysozyme, ribonuclease A, cytochrome C, bovine pancreatic trypsin inhibitor, and chymotrypsinogen were baselinely separated even at pH 8.0. The separation efficiency and analysis reproducibility demonstrated that the QC coatings were efficient in minimizing the adsorption of basic proteins on the fused silica capillary. The successful performance was further demonstrated for biosample analysis.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, PR China.
authors: Lingguo
authors: L
authors: Zhao
authors: Jinping
authors: J
authors: Zhou
authors: Huan
authors: H
authors: Xie
authors: Dihui
authors: D
authors: Huang
authors: Ping
authors: P
authors: Zhou
mesh_terms: D002412:Cations / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry*; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results
chemical_list: D002412:Cations; D011506:Proteins; D002482:Cellulose