title: Sec5 and Exo84 mediate distinct aspects of RalA-dependent cell polarization.
abstract: Metastasis is a complex process during which several gross cellular changes occur. Cells must dissociate from the tumor mass and gain the ability to degrade extracellular matrix and migrate in order to ultimately attach and form a satellite tumor. Regulation of the actin cytoskeleton is an indispensible aspect of cell migration, and many different factors have been implicated in this process. We identified interactions between RalA and its effectors in the Exocyst complex as directly necessary for migration and invasion of prostate cancer tumor cells. Blocking RalA-Exocyst binding caused significant morphological changes and defects in single and coordinated cell migration.
authors: Department of Anatomy and Cell Biology Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America.
authors: C Clayton
authors: CC
authors: Hazelett
authors: Charles
authors: C
authors: Yeaman
mesh_terms: D000199:Actins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology*; D048430:Cell Shape / Q000502:physiology; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009362:Neoplasm Metastasis / Q000235:genetics; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*; D020725:ral GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; C470182:EXOC2 protein, human; C480857:EXOC8 protein, human; D033921:Vesicular Transport Proteins; C492198:RALA protein, human; D020725:ral GTP-Binding Proteins