title: CD133 is a marker for long-term repopulating murine epidermal stem cells.
abstract: Maintenance, repair, and renewal of the epidermis are thought to depend on a pool of dedicated epidermal stem cells (EpiSCs). Like for many somatic tissues, isolation of a nearly pure population of stem cells is a primary goal in cutaneous biology. We used a quantitative transplantation assay, using injection of keratinocytes into subcutis combined with limiting dilution analysis, to assess the long-term repopulating ability of putative murine EpiSC populations. Putative EpiSC populations were isolated by FACS sorting. The CD133(+) population and the subpopulation of CD133(+) cells that exhibits high mitochondrial membrane potential (DΨm(hi)) were enriched for long-term repopulating EpiSCs versus unfractionated cells (3.9- and 5.2-fold, respectively). Evidence for self-renewal capacity was obtained by serial transplantation of long-term epidermal repopulating units derived from CD133(+) and CD133(+)ΔΨm(hi) keratinocytes. CD133(+) keratinocytes were multipotent and produced significantly more hair follicles than CD133(-) cells. CD133(+) cells were a subset of the previously described integrin α6(+)CD34(+) bulge cell population, and 28.9±8.6% were label-retaining cells. Thus, murine keratinocytes within the CD133(+) and CD133(+)ΔΨm(hi) populations contain EpiSCs that regenerate the epidermis for the long term, are self-renewing, multipotent, and label-retaining cells.
authors: Department of Dermatology, University of California, San Francisco, San Francisco, California, USA.
authors: Alexandra
authors: A
authors: Charruyer
authors: Lauren R
authors: LR
authors: Strachan
authors: Lili
authors: L
authors: Yue
authors: Alexandra S
authors: AS
authors: Toth
authors: Gary
authors: G
authors: Cecchini
authors: Maria L
authors: ML
authors: Mancianti
authors: Ruby
authors: R
authors: Ghadially
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D000078404:Epidermal Cells; D004817:Epidermis / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics; D039503:Integrin alpha6 / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology* / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008822:Mice, Transgenic; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology; D010455:Peptides / Q000378:metabolism*; D012038:Regeneration / Q000502:physiology; D016038:Skin Transplantation; D014184:Transplantation, Homologous
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D015415:Biomarkers; D006023:Glycoproteins; D039503:Integrin alpha6; D010455:Peptides; C000606024:Prom1 protein, mouse; D049452:Green Fluorescent Proteins