title: The immunohistochemical characterization of MMP-2, MMP-10, TIMP-1, TIMP-2, and podoplanin in oral squamous cell carcinoma.
abstract: OBJECTIVE
  The aim of this study was to immunohistochemically evaluate the expression of matrix metalloproteinase (MMP)-1, MMP- 2, tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, and podoplanin in oral squamous cell carcinoma (OSCC). Immunohistochemical staining of podoplanin-positive lymphatic vessel density (LVD) was also assessed.
  
  
  METHODS
  Forty cases of OSCC were analyzed by immunohistochemistry.
  
  
  RESULTS
  MMP-2, MMP-10, TIMP-1, TIMP-2, and podoplanin were detected in each of the 40 OSCC cases. The expression of MMP-2 was significantly correlated with histologic grade. The expression of podoplanin was positively correlated with gender and negatively correlated with tumor size. A significant positive correlation was also detected between LVD and the presence of lymph node metastases, gender, age, and diameter of the lymph node (if involved), as well as histologic grade.
  
  
  CONCLUSIONS
  The results are suggestive of important roles that MMP-2, MMP-10, TIMP-2, and podoplanin play in pathologic processes of OSCC, including invasion. Our findings also suggest that LVD may play a role in lymphatic metastasis and tumor progression.
authors: Department of Oral and Maxillofacial Pathology, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran.
authors: Fatemeh
authors: F
authors: Mashhadiabbas
authors: Faranak
authors: F
authors: Mahjour
authors: Seyyed Babak
authors: SB
authors: Mahjour
authors: Forouzandeh
authors: F
authors: Fereidooni
authors: Fatemeh Sadat
authors: FS
authors: Hosseini
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007492:Iran; D008207:Lymphatic Metastasis; D042601:Lymphatic Vessels / Q000473:pathology; D008297:Male; D053506:Matrix Metalloproteinase 10 / Q000032:analysis / Q000096:biosynthesis; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000096:biosynthesis; D020782:Matrix Metalloproteinases / Q000032:analysis* / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000096:biosynthesis; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D009361:Neoplasm Invasiveness; D012044:Regression Analysis; D012189:Retrospective Studies; D012737:Sex Factors; D018709:Statistics, Nonparametric; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis / Q000096:biosynthesis; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000032:analysis / Q000096:biosynthesis; D000098853:Podoplanin
chemical_list: D053506:Matrix Metalloproteinase 10; D020778:Matrix Metalloproteinase 2; D020782:Matrix Metalloproteinases; D008562:Membrane Glycoproteins; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; C117468:PDPN protein, human; D000098853:Podoplanin