title: Effects of melatonin on testis histology, oxidative stress and spermatogenesis after experimental testis ischemia-reperfusion in rats.
abstract: BACKGROUND
  Testicular torsion due to oxidative stress results in infertility and testicular damage which can be preventable an important health problem worldwide.
  
  
  OBJECTIVE
  The purpose of the present study was to investigate the changes of malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS) levels; histopathological alterations; morphology, concentration and motilities of the sperm in post ischemic reperfused (I/R) testis tissue.
  
  
  METHODS
  Forty adult male Wistar rats were carried out and were randomized to five groups; (1) Control group, (2) Ipsilateral left testis ischemia, (3) Melatonin plus ipsilateral left testis ischemia, (4) Contralateral right testis ischemia, 5. Melatonin plus contralateral right testis ischemia. After 1 h ischemia and 24 h perfusion; MDA, TAS and TOS levels were measured, histopathological alterations were determined using by Johnsen's score (JS) and sperm morphology, concentration, motility were examined.
  
  
  RESULTS
  MDA, TAS and TOS levels of the testis tissue did not change in all groups (p > 0.05 for all). JS was decreased in I/R group and melatonin treatment reversed histopathological changes and increased JS both in ipsilateral and contralateral testis. Abnormal sperm rate significantly increased in I/R group and melatonin administration changed abnormal sperm rate to normal.
  
  
  CONCLUSIONS
  As a result, the present study demonstrated that testicular damage occurs following I/R without an increase of MDA, TAS and TOS levels. Our results also suggested that melatonin is a potent antioxidant agent in preventing testicular I/R injury, as shown by increased JS and changed abnormal sperm rate.
authors: Department of Histology and Embryology, Faculty of Medicine, Harran University, Sanliurfa, Turkey. mkoksal@harran.edu.tr
authors: M
authors: M
authors: Koksal
authors: E
authors: E
authors: Oğuz
authors: F
authors: F
authors: Baba
authors: M Ali
authors: MA
authors: Eren
authors: H
authors: H
authors: Ciftci
authors: M E
authors: ME
authors: Demir
authors: Z
authors: Z
authors: Kurcer
authors: G
authors: G
authors: Take
authors: F
authors: F
authors: Aral
authors: A R
authors: AR
authors: Ocak
authors: N
authors: N
authors: Aksoy
authors: T
authors: T
authors: Ulas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004195:Disease Models, Animal; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013086:Spermatic Cord Torsion / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D008550:Melatonin