title: TNF-α influences the lateral dynamics of TNF receptor I in living cells.
abstract: In mammalian cells, inflammation is mainly mediated by the binding of tumor necrosis factor alpha to tumor necrosis factor receptor 1. In this study, we investigated lateral dynamics of TNF-R1 before and after ligand binding using high-density single-particle tracking in combination with photoactivated localization microscopy. Our single-molecule data indicates the presence of tumor necrosis factor receptor 1 with different mobilities in the plasma membrane, suggesting different molecular organizations. Cholesterol depletion led to a decrease of slow receptor species and a strong increase in the average diffusion coefficient. Moreover, as a consequence of tumor necrosis factor-alpha treatment, the mean diffusion coefficient moderately increased while its distribution narrowed. Based on our observation, we propose a refined mechanism on the structural arrangement and activation of tumor necrosis factor receptor 1 in the plasma membrane.
authors: Julius-Maximilians-Universität, Department of Biotechnology & Biophysics, Würzburg, Germany.
authors: Meike
authors: M
authors: Heidbreder
authors: Christin
authors: C
authors: Zander
authors: Sebastian
authors: S
authors: Malkusch
authors: Darius
authors: D
authors: Widera
authors: Barbara
authors: B
authors: Kaltschmidt
authors: Christian
authors: C
authors: Kaltschmidt
authors: Deepak
authors: D
authors: Nair
authors: Daniel
authors: D
authors: Choquet
authors: Jean-Baptiste
authors: JB
authors: Sibarita
authors: Mike
authors: M
authors: Heilemann
mesh_terms: D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000172:deficiency / Q000378:metabolism; D004058:Diffusion / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008853:Microscopy; D008954:Models, Biological; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha; D002784:Cholesterol