title: Hydrogen peroxide and nitric oxide mediated cold- and dehydration-induced myo-inositol phosphate synthase that confers multiple resistances to abiotic stresses.
abstract: myo-Inositol phosphate synthase (MIPS) is the key enzyme of myo-inositol synthesis, which is a central molecule required for cell metabolism and plant growth as a precursor to a large variety of compounds. A full-length fragment of MfMIPS1 cDNA was cloned from Medicago falcata that is more cold-tolerant than Medicago sativa. While MfMIPS1 transcript was induced in response to cold, dehydration and salt stress, MIPS transcript and myo-inositol were maintained longer and at a higher level in M. falcata than in M. sativa during cold acclimation at 5 °C. MfMIPS1 transcript was induced by hydrogen peroxide (H(2) O(2)) and nitric oxide (NO), but was not responsive to abscisic acid (ABA). Pharmacological experiments revealed that H(2) O(2) and NO are involved in the regulation of MfMIPS1 expression by cold and dehydration, but not by salt. Overexpression of MfMIPS1 in tobacco increased the MIPS activity and levels of myo-inositol, galactinol and raffinose, resulting in enhanced resistance to chilling, drought and salt stresses in transgenic tobacco plants. It is suggested that MfMIPS1 is induced by diverse environmental factors and confers resistance to various abiotic stresses.
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China.
authors: Jiali
authors: J
authors: Tan
authors: Congying
authors: C
authors: Wang
authors: Bin
authors: B
authors: Xiang
authors: Ruihong
authors: R
authors: Han
authors: Zhenfei
authors: Z
authors: Guo
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D050260:Carbohydrate Metabolism / Q000187:drug effects / Q000235:genetics; D003001:Cloning, Molecular; D003080:Cold Temperature; D003681:Dehydration; D005615:Freezing; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007477:Ions; D029902:Medicago / Q000201:enzymology* / Q000235:genetics; D007296:Myo-Inositol-1-Phosphate Synthase / Q000235:genetics / Q000378:metabolism*; D009569:Nitric Oxide / Q000494:pharmacology*; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D010802:Phylogeny; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000502:physiology*
chemical_list: D007477:Ions; D012333:RNA, Messenger; D009569:Nitric Oxide; D012965:Sodium Chloride; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D007296:Myo-Inositol-1-Phosphate Synthase