title: Screening and comparison of differentially expressed genes between one MDR-TB strain and the virulent M. tuberculosis H37Rv.
abstract: To screen and compare the differentially expressed genes between one MDR-TB strain separated from one child patient and the virulent Mycobacterium tuberculosis H37Rv, suppression subtractive hybridization (SSH) technology was used to build a library of cDNAs that were differentially expressed in the MDR and H37Rv. From this cDNA library, genes that were expressed in the MDR-TB but not in the H37Rv were selected for gene sequencing and homology analysis; 113 positive clones were obtained, their cDNA fragments were sequenced, and homology analysis was performed. Four novel sequences were identified. The results provide a partial list of genes differentially expressed in MDR-TB and four novel genes were found. Identification of these genes may contribute to our understanding of MDR-TB development.
authors: The Infection and Digestology Department of Affiliated Children's Hospital, PR China. 371414672@qq.com
authors: Yunling
authors: Y
authors: Zhang
authors: Gaihuan
authors: G
authors: Zheng
authors: Yuting
authors: Y
authors: Wang
authors: Junhua
authors: J
authors: Chen
authors: Chaomin
authors: C
authors: Zhu
authors: Ruixi
authors: R
authors: Liu
authors: Zhe
authors: Z
authors: Peng
authors: Qizhi
authors: Q
authors: Li
authors: Linlin
authors: L
authors: Xing
mesh_terms: D001483:Base Sequence; D002648:Child; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D020224:Expressed Sequence Tags; D015870:Gene Expression; D015723:Gene Library; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D013045:Species Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000382:microbiology; D014774:Virulence / Q000235:genetics
chemical_list: D004269:DNA, Bacterial