title: [Dynamic correlation of TNF-α and    IL-10 with  myocardial remodeling induced by pressure overload in rats].
abstract: OBJECTIVE
  To observe the expression changes of tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) during the developing process of pressure overload-induced myocardial remodeling in rats, and investigate the dynamic correlation between TNF-α and left ventricular mass index (LVMI) as well as myocardial collagen volume fraction (CVF).
  
  
  METHODS
  The rats (n=56) were randomly divided into control group, sham operation group and model group. Animal models were induced by abdominal aortic constriction (AAC). AAC group was further divided into 2 weeks, 4 weeks, 8 weeks and 12 weeks groups. Dynamic expression changes of TNF-α and IL-10 were tested by enzyme-linked immunosorbent assay(ELISA), and myocardial collagen fiber was observed by Mallory's staining.
  
  
  RESULTS
  Myocardial cells became increasingly disarranged, filaments broken, intercellular space increased, and collagen fiber accumulated 4 weeks after operation. Furthermore, heart failure occurred 12 weeks after operation. ABC-ELISA results showed that TNF-α expression in myocardium increased at 4, 8, 12-week operation groups in a time-dependent fashion compared with control group (P<0.01); Correlation analysis indicated the expression of TNF-α in myocardium was positively correlated with LVMI (r=0.582, P<0.01) and CVF (r=0.932, P<0.01); IL-10 expression in myocardium among groups were similar, but the ratio of TNF-α and IL-10 increased in a time-dependent manner, from (1.79 ± 0.19) ng/mL (2 weeks) up to (2.85 ± 0.24) ng/mL(12 weeks) as compared with control group (1.74 ± 0.24) ng/mL (P<0.01).
  
  
  CONCLUSIONS
  The degree of myocardial remodeling plays a key role in heart function deterioration, and the TNF-α expression up-regulation in a time-dependent manner and the disproportion of TNF-α and IL-10 is one of important molecular mechanisms.
authors: Department of Pharmacology, Xi'an Medical University, China. jrzeng988@hotmail.com
authors: Ju-rong
authors: JR
authors: Zeng
authors: Xiao-li
authors: XL
authors: Xu
authors: Xiao-jiang
authors: XJ
authors: Yu
authors: Jin
authors: J
authors: Hou
authors: Tian-jiao
authors: TJ
authors: Xu
authors: Man
authors: M
authors: Mi
authors: Dong-ling
authors: DL
authors: Li
authors: Wei-jin
authors: WJ
authors: Zang
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006333:Heart Failure / Q000235:genetics / Q000378:metabolism; D006439:Hemodynamics; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D020257:Ventricular Remodeling / Q000235:genetics
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D003094:Collagen