title: SUMOylation represses Nanog expression via modulating transcription factors Oct4 and Sox2.
abstract: Nanog is a pivotal transcription factor in embryonic stem (ES) cells and is essential for maintaining the pluripotency and self-renewal of ES cells. SUMOylation has been proved to regulate several stem cell markers' function, such as Oct4 and Sox2. Nanog is strictly regulated by Oct4/Sox2 heterodimer. However, the direct effects of SUMOylation on Nanog expression remain unclear. In this study, we reported that SUMOylation repressed Nanog expression. Depletion of Sumo1 or its conjugating enzyme Ubc9 increased the expression of Nanog, while high SUMOylation reduced its expression. Interestingly, we found that SUMOylation of Oct4 and Sox2 regulated Nanog in an opposing manner. SUMOylation of Oct4 enhanced Nanog expression, while SUMOylated Sox2 inhibited its expression. Moreover, SUMOylation of Oct4 by Pias2 or Sox2 by Pias3 impaired the interaction between Oct4 and Sox2. Taken together, these results indicate that SUMOylation has a negative effect on Nanog expression and provides new insights into the mechanism of SUMO modification involved in ES cells regulation.
authors: College of life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
authors: Yongyan
authors: Y
authors: Wu
authors: Zekun
authors: Z
authors: Guo
authors: Haibo
authors: H
authors: Wu
authors: Xiaohai
authors: X
authors: Wang
authors: Lixia
authors: L
authors: Yang
authors: Xiaoyan
authors: X
authors: Shi
authors: Juan
authors: J
authors: Du
authors: Bo
authors: B
authors: Tang
authors: Wenzhong
authors: W
authors: Li
authors: Liping
authors: L
authors: Yang
authors: Yong
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D051379:Mice; D041681:NIH 3T3 Cells; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D050828:Protein Inhibitors of Activated STAT / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism*; D058207:Sumoylation / Q000235:genetics / Q000502:physiology*
chemical_list: D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; C109279:Pias3 protein, mouse; D050828:Protein Inhibitors of Activated STAT; D055748:SOXB1 Transcription Factors