title: Myosin II-dependent exclusion of CD45 from the site of Fcγ receptor activation during phagocytosis.
abstract: Fcγ receptor (FcγR)-mediated phagocytosis requires myosin II activity. Here we show that myosin II contributes to FcγR activation and subsequent F-actin assembly at the nascent phagocytic cup. Inhibition of myosin II attenuates phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FcγR and binding of Syk to the ITAM. Furthermore, FcγR clusters independently of myosin II activity at the phagocytic cup, from which the receptor-like protein tyrosine phosphatase CD45 is excluded depending on myosin II activity. These findings suggest that myosin II-dependent segregation of CD45 from FcγR facilitates phosphorylation of the ITAM and triggers phagocytosis.
authors: Laboratory of Cell Signaling, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors: Shota
authors: S
authors: Yamauchi
authors: Keiko
authors: K
authors: Kawauchi
authors: Yasuhiro
authors: Y
authors: Sawada
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D061625:Immunoreceptor Tyrosine-Based Activation Motif; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000737:chemistry / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D024462:Myosin Type II / Q000378:metabolism*; D010587:Phagocytosis / Q000276:immunology* / Q000502:physiology*; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D017452:Receptors, IgG / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction; D000072377:Syk Kinase
chemical_list: D000199:Actins; D047908:Intracellular Signaling Peptides and Proteins; D017452:Receptors, IgG; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; C000606897:Syk protein, mouse; D017493:Leukocyte Common Antigens; D024462:Myosin Type II