title: Hobi-like pestivirus: both biotypes isolated from a diseased animal.
abstract: A Hobi-like pestivirus pair consisting of cytopathogenic (cp) and non-cytopathogenic (noncp) strains, Italy 83/10cp and Italy 83/10ncp, was isolated from the lung of a heifer that died of respiratory disease. The noncp and cp viruses were isolated on Madin-Darby bovine kidney cells and separated by plaque purification and end point dilution. Analysis of the nearly full-length genomes revealed that the two viruses were very closely related to each other and to the noncp Hobi-like strain Italy 1/10-1, which had been isolated a few weeks earlier from the same herd. One major difference between noncp and cp viruses concerned the presence of a cellular Jiv sequence in the 3' domain of the NS2-encoding region of the cp strain. This is the first study, to our knowledge, reporting the isolation and molecular characterization of a Hobi-like virus pair.
authors: Department of Veterinary Public Health, University of Bari, Valenzano (Bari), Italy.
authors: Nicola
authors: N
authors: Decaro
authors: Department of Veterinary Public Health, University of Bari, Valenzano (Bari), Italy.
authors: Viviana
authors: V
authors: Mari
authors: Department of Veterinary Public Health, University of Bari, Valenzano (Bari), Italy.
authors: Pierfrancesco
authors: P
authors: Pinto
authors: Department of Veterinary Public Health, University of Bari, Valenzano (Bari), Italy.
authors: Maria Stella
authors: MS
authors: Lucente
authors: Department of Veterinary Public Health, University of Bari, Valenzano (Bari), Italy.
authors: Rossana
authors: R
authors: Sciarretta
authors: Department of Veterinary Public Health, University of Bari, Valenzano (Bari), Italy.
authors: Francesco
authors: F
authors: Cirone
authors: Department of Veterinary Public Health, University of Bari, Valenzano (Bari), Italy.
authors: Maria Loredana
authors: ML
authors: Colaianni
authors: Department of Veterinary Public Health, University of Bari, Valenzano (Bari), Italy.
authors: Gabriella
authors: G
authors: Elia
authors: Institut für Virologie, Justus Liebig Universität Giessen, Giessen, Germany.
authors: Heinz-Jürgen
authors: HJ
authors: Thiel
authors: Department of Veterinary Public Health, University of Bari, Valenzano (Bari), Italy.
authors: Canio
authors: C
authors: Buonavoglia
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D005260:Female; D008969:Molecular Sequence Data; D010576:Pestivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D018182:Pestivirus Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D017361:Viral Nonstructural Proteins