title: Preparation and characterization of enrofloxacin-imprinted monolith prepared with crowding agents.
abstract: Molecular crowding is a new approach to promoting molecular imprinting more efficiently. In this work, this concept was applied to the preparation of imprinted monoliths in the presence of molecular crowding agent for stabilizing binding sites and improving molecular recognition. The imprinted monolithic column was synthesized using a mixture of enrofloxacin (template), methacrylic acid, ethylene glycol dimethacrylate, and polystyrene (molecular crowding agent). Some polymerization factors, such as template-monomer molar ratio and the composition of the porogen, on the imprinting effect of resulting MIP monolith were systematically investigated. Moreover, a stoichiometric displacement model for retention was successfully applied to evaluate the interaction between the solute and the stationary phase. Compared with the MIP prepared by conventional polymerization, the molecular crowding-based monolithic MIPs showed higher selectivity. The results suggested that molecular crowding is a powerful strategy to increase the effect of molecular imprinting.
authors: Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 40-1 South Beijing Road, Urumqi 830011, Xinjiang, China; Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors: Xiao-Xia
authors: XX
authors: Li
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors: Li-Hong
authors: LH
authors: Bai
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors: Huan
authors: H
authors: Wang
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors: Jia
authors: J
authors: Wang
authors: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China. Electronic address: huangyp100@163.com.
authors: Yan-Ping
authors: YP
authors: Huang
authors: Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 40-1 South Beijing Road, Urumqi 830011, Xinjiang, China; Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China. Electronic address: zhaoshengliu@sohu.com.
authors: Zhao-Sheng
authors: ZS
authors: Liu
mesh_terms: D000096:Acetone / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000737:chemistry*; D008968:Molecular Conformation; D054802:Molecular Imprinting / Q000379:methods*; D016062:Porosity
chemical_list: D000097:Acetonitriles; D024841:Fluoroquinolones; D000096:Acetone; D000077422:Enrofloxacin; D004121:Dimethyl Sulfoxide; C032159:acetonitrile