title: Neurotoxic effects evoked by cyanobacterial extracts suggest multiple receptors involved in electrophysiological responses of molluscan (CNS, heart) models.
abstract: The responses of the snail central neurons (Helix pomatia, Lymnaea stagnalis) and the isolated Helix heart were characterized evoked by cyanobacterial extracts (Cylindrospermopsis raciborskii ACT strains) isolated from Lake Balaton (Hungary). The nicotinergic acetylcholine (ACh) receptors in the CNS (both excitatory and inhibitory) were blocked by the extracts of ACT 9502 and ACT 9505 strains and the anatoxin- a (homoanatoxin-a) producing reference strain of Oscillatoria sp. (PCC 6506), similar to the inhibitory effects of the pure anatoxin-a. The enhancement of the ACh responses by the ACT 9504 extract suggests additional, probably acetylcholine esterase inhibitory mechanisms. On the isolated Helix heart the crude ACT 9505 and PCC 6506 extracts evoked frequency increase and transient twitch contraction, opposite to the ACh evoked heart relaxation. Anatoxin-a similarly contracted the heart but did not increase its contration frequency. These data suggest the involvement of some non-cholinergic mechanisms, acting very likely by direct modulation of the electrical or contractile system of the isolated heart. Diversity of the effects evoked by the cyanobacterial extracts in the CNS and heart suggest pharmacologically different neuroactive components among the secondary metabolites of the cyanobacteria acting on both (anatoxin-a like) cholinergic and (unidentified) non-cholinergic receptors.
authors: MTA Centre for Ecological Research, Balaton Limnological Institute, P.O. Box 35, H-8237 Tihany, Hungary. vehovszky.agnes@okologia.mta.hu
authors: Agnes
authors: A
authors: Vehovszky
authors: A W
authors: AW
authors: Kovács
authors: Henriette
authors: H
authors: Szabó
authors: J
authors: J
authors: Győri
authors: Anna
authors: A
authors: Farkas
mesh_terms: D000818:Animals; D001688:Biological Products / Q000633:toxicity*; D000087522:Cyanobacteria Toxins; D046891:Cylindrospermopsis / Q000737:chemistry*; D055724:Electrophysiological Phenomena / Q000187:drug effects*; D006321:Heart / Q000187:drug effects; D006372:Helix, Snails / Q000166:cytology / Q000502:physiology*; D066298:In Vitro Techniques; D008195:Lymnaea / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000187:drug effects*; D014326:Tropanes / Q000633:toxicity
chemical_list: D001688:Biological Products; D000087522:Cyanobacteria Toxins; D014326:Tropanes; C509783:anatoxin a