title: The effect of glucose concentrations in the medium on expression of insulin receptors in human lymphocytes B and T: an in vitro study.
abstract: BACKGROUND
  Insulin is one of the most-known factors that influence the intensity of cell-bound glucose transport. However, in order to react to this hormone, a cell needs specific receptors present in its membrane. The aim of this work was to investigate the insulin receptor expression in B and T cells under incubation with pathological glucose concentrations, respond hyperglycemia and hypoglycemia.
  
  
  METHODS
  Isolated B and T cells were cultivated in different concentrations of glucose (high, low and normal). The expression of insulin receptors was investigated using methods of immunocytochemistry and flow cytometry.
  
  
  RESULTS
  Incubation for 24 h of lymphocytes in pathological glucose concentrations seems to only have a slight influence on the expression of insulin receptors. No insulin receptor expression has been found in lymphocytes T incubated in both pathological concentrations of glucose. Different concentrations of glucose in the incubation medium were found to only marginally influence expression of insulin receptors in lymphocytes B.
  
  
  CONCLUSIONS
  Pathological concentrations of glucose in medium cause a decrease in the percent of cells which show expression of insulin receptors in comparison with normal glucose concentration. Thus, it appears highly probable that the insulin receptors did not arise under pathological glucose concentration in these cells de novo, but in little percent lymphocytes have existed there earlier, before the incubation.
authors: Chair of General Biology and Parasitology, Center for Biostructure Research, Medical University of Warsaw, Warsaw, Poland.
authors: Bożenna
authors: B
authors: Oleszczak
authors: Leszek
authors: L
authors: Szablewski
authors: Monika
authors: M
authors: Pliszka
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D011972:Receptor, Insulin / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
chemical_list: D003470:Culture Media; D011972:Receptor, Insulin; D005947:Glucose