title: Major metabolite of F2-isoprostane in urine may be a more sensitive biomarker of oxidative stress than isoprostane itself.
abstract: BACKGROUND
  There is limited literature on the contributors to isoprostane metabolite 2,3-dinor-5,6-dihydro-15-F(2t)-isoprostane (15-F(2t)-IsoP-M) compared with F(2)-isoprostanes (F(2)-IsoPs) as an oxidative stress biomarker.
  
  
  OBJECTIVE
  The objective of this study was to investigate whether plasma concentrations of antioxidants, urinary excretion rates of polyphenols, and antioxidants in food and dietary supplements are attributable to both urinary F(2)-IsoP and 15-F(2t)-IsoP-M concentrations.
  
  
  METHODS
  Dietary intake information and blood and urine samples were obtained from 845 healthy middle-aged and elderly female participants of the Shanghai Women's Health Study. Urinary isoprostanes (F(2)-IsoPs and 15-F(2t)-IsoP-M) were measured and adjusted for creatinine concentrations.
  
  
  RESULTS
  Urinary 15-F(2t)-IsoP-M and F(2)-IsoP concentrations were lower in subjects who used a multivitamin. Lower F(2)-IsoP concentrations were observed in ginseng users, whereas lower concentrations of 15-F(2t)-IsoP-M were shown in subjects who used a vitamin E supplement. Plasma concentrations of several antioxidants (ie, β-carotenes, both trans and cis β-carotenes, lycopene other than trans, 5-cis and 7-cis isomers, cis anhydrolutein, and cis β-cryptoxanthin) were inversely associated with 15-F(2t)-IsoP-M but not with F(2)-IsoPs, whereas β-, γ-, and δ-tocopherols were positively associated with 15-F(2t)-IsoP-M but not with F(2)-IsoPs. Urinary polyphenol quercetin was positively associated with both F(2)-IsoPs and 15-F(2t)-IsoP-M.
  
  
  CONCLUSIONS
  The results suggest that the F(2)-IsoP major metabolite 15-F(2t)-IsoP-M may be a more sensitive marker of endogenous oxidative stress status than are F(2)-IsoPs in the assessment of effects of antioxidants on age-related diseases.
authors: Divisions of Epidemiology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors: Tsogzolmaa
authors: T
authors: Dorjgochoo
authors: Yu-Tang
authors: YT
authors: Gao
authors: Wong-Ho
authors: WH
authors: Chow
authors: Xiao-ou
authors: XO
authors: Shu
authors: Gong
authors: G
authors: Yang
authors: Qiuyin
authors: Q
authors: Cai
authors: Nathaniel
authors: N
authors: Rothman
authors: Hui
authors: H
authors: Cai
authors: Honglan
authors: H
authors: Li
authors: Xinqing
authors: X
authors: Deng
authors: Adrian
authors: A
authors: Franke
authors: L Jackson
authors: LJ
authors: Roberts
authors: Ginger
authors: G
authors: Milne
authors: Wei
authors: W
authors: Zheng
authors: Qi
authors: Q
authors: Dai
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000008:administration & dosage; D015415:Biomarkers / Q000652:urine*; D003430:Cross-Sectional Studies; D019587:Dietary Supplements; D028441:F2-Isoprostanes / Q000652:urine*; D005260:Female; D005419:Flavonoids / Q000652:urine; D005638:Fruit; D006801:Humans; D028421:Isoprostanes / Q000652:urine*; D016014:Linear Models; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D059808:Polyphenols / Q000652:urine; D013662:Tea / Q000737:chemistry; D024505:Tocopherols / Q000097:blood; D014675:Vegetables; D014801:Vitamin A / Q000097:blood; D014815:Vitamins / Q000008:administration & dosage
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D028441:F2-Isoprostanes; D005419:Flavonoids; D028421:Isoprostanes; D059808:Polyphenols; D013662:Tea; D014815:Vitamins; D014801:Vitamin A; D024505:Tocopherols