title: Simultaneous diffuse reflectance infrared determination of clavulanic acid and amoxicillin using multivariate calibration techniques.
abstract: A method for simultaneous determination of clavulanic acid (CA) and amoxicillin (AMO) in commercial tablets was developed using diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and multivariate calibration. Twenty-five samples (10 commercial and 15 synthetic) were used as a calibration set and 15 samples (10 commercial and 5 synthetic) were used for a prediction set. Calibration models were developed using partial least squares (PLS), interval PLS (iPLS), and synergy interval PLS (siPLS) algorithms. The best algorithm for CA determination was siPLS model with spectra divided in 30 intervals and combinations of 2 intervals. This model showed a root mean square error of prediction (RMSEP) of 5.1 mg g(-1). For AMO determination, the best siPLS model was obtained with spectra divided in 10 intervals and combinations of 4 intervals. This model showed a RMSEP of 22.3 mg g(-1). The proposed method was considered as a suitable for the simultaneous determination of CA and AMO in commercial pharmaceuticals products.
authors: Departamento de Química, Universidade Federal de Santa Maria, Campus, 97105-900, Santa Maria, Rio Grande do Sul, Brazil.
authors: Aline Lima Hermes
authors: AL
authors: Müller
authors: Rochele Sogari
authors: RS
authors: Picoloto
authors: Marco Flores
authors: MF
authors: Ferrão
authors: Fabiana Ernestina Barcellos
authors: FE
authors: da Silva
authors: Edson Irineu
authors: EI
authors: Müller
authors: Erico Marlon de Moraes
authors: EM
authors: Flores
mesh_terms: D000465:Algorithms; D019980:Amoxicillin-Potassium Clavulanate Combination / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002138:Calibration; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013607:Tablets
chemical_list: D000900:Anti-Bacterial Agents; D013607:Tablets; D019980:Amoxicillin-Potassium Clavulanate Combination