title: Development of an ultra-low volume flow cell for surface plasmon resonance detection in a miniaturized capillary electrophoresis system.
abstract: A miniaturized capillary electrophoresis system coupled to a surface plasmon resonance (SPR) sensor on a microfluidic platform fabricated from PDMS is detailed. A previously described split-flow injection technique is first utilized to manipulate sample into the microfluidic chip, followed by separation within the fused-silica capillary and final off-capillary detection of analytes via SPR. Instead of using commercial SPR flow cells requiring relatively large detection volumes, samples of less than 1 nL volume are utilized. The interface between the CE system and SPR sensor made it possible to detect minute volumes of sample with minimal dispersion. The flow cell has the potential to be applicable to miniaturized flow-injection (FI) systems where submicroliter volumes of sample are frequently only available for analysis. The components present in solution, but not bound to the sensor surface, were also investigated. The sensitivity of the CE-SPR system was similar to that found in UV-spectrometric instruments and nonchromophoric components could also be measured.
authors: Department of Chemistry and Biochemistry, California State University, Los Angeles, CA 90032-8202, USA.
authors: Attila
authors: A
authors: Gaspar
authors: Frank A
authors: FA
authors: Gomez
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D008904:Miniaturization / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D004129:Dimethylpolysiloxanes; D000431:Ethanol; C013830:baysilon