title: Expression of PCNA, ICAM-1, and vimentin in lens epithelial cells of cataract patients with and without type 2 diabetes.
abstract: OBJECTIVE
  In order to explore the pathological feature in the progression of cataract with type 2 diabetes, we compared the expression of proliferating cell nuclear antigen (PCNA), intercellular adhesion molecule-1 (ICAM-1), and vimentin in lens epithelial cells (LECs) of cataract patients with and without type 2 diabetes.
  
  
  METHODS
  The indirect immunoperoxidase technique was performed on anterior capsules obtained from 25 patients with type 2 diabetes (DM group) and 25 patients without diabetes (control group). Immunohistochemical difference in the expression of PCNA, ICAM-1, and vimentin in LECs between the 2 groups was investigated.
  
  
  RESULTS
  PCNA expression was decreased (P=0.036) but ICAM-1 expression was significantly increased (P<0.0001) in the DM group as compared with that in the control group. No difference was noted in the expression of vimentin between the 2 groups (P=0.264).
  
  
  CONCLUSIONS
  Decreased proliferation of LECs and increased expression of ICAM-1 in LECs might play an important role in the progression of cataract with type 2 diabetes.
authors: Department of Ophthalmology, Tokai University School of Medicine, 143 Simokasuya, Isehara, Kanagawa 259-1193, Japan. cn6283annie@yahoo.co.jp
authors: Haiyan
authors: H
authors: Fan
authors: Takahiro
authors: T
authors: Suzuki
authors: Masafumi
authors: M
authors: Ogata
authors: Yoshihiro
authors: Y
authors: Nakagawa
authors: Hiromi
authors: H
authors: Onouchi
authors: Kenji
authors: K
authors: Kawai
mesh_terms: D000368:Aged; D002386:Cataract / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000378:metabolism; D018450:Disease Progression; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007903:Lens Capsule, Crystalline / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism*
chemical_list: C573420:ICAM1 protein, human; D018809:Proliferating Cell Nuclear Antigen; D014746:Vimentin; D018799:Intercellular Adhesion Molecule-1