title: [Simultaneous determination of hypocrellin A, hypocrellin B, and hypocrellin C by HPLC].
abstract: OBJECTIVE
  To develop a high-performance liquid chromatography (HPLC) method for simultaneous determination of hypocrellin A, hypocrellin B, and hypocrellin C.
  
  
  METHODS
  The separation was carried out on a Kromasil C18 (4.6 mm x 250 mm, 5 micrm) colum eluted with in mobile phases of water containing 0.5% glacial acetic acid and acetonitrile. The column temperature was 35 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was set at 265 nm.
  
  
  RESULTS
  The three compounds were well separated. Calibration curves of hypocrellin A, hypocrellin B, and hypocrellin C showed good linear relationship RSD > 2.0%. The average recoveries of the hypocrellin A, hypocrellin B, and hypocrellin C were 101.8%, 102.3%, 100.0%, respectively.
  
  
  CONCLUSIONS
  The developed method is simple, accurate, and repeatable, and can be readily used as valid tool for the quality control of Hypocrella bambusae.
authors: Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.
authors: Ming
authors: M
authors: Kong
authors: Zhanli
authors: Z
authors: Chen
authors: Zhiqi
authors: Z
authors: Yin
authors: Jian
authors: J
authors: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D006999:Hypocreales / Q000737:chemistry*; D010569:Perylene / Q000031:analogs & derivatives* / Q000032:analysis; D019800:Phenol; D011809:Quinones / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D011809:Quinones; C072579:hypocrellin C; D019800:Phenol; D010569:Perylene; C048388:hypocrellin A; C064522:hypocrellin B