title: A convenient and robust in vivo reporter system to monitor gene expression in the human pathogen Helicobacter pylori.
abstract: Thirty years of intensive research have significantly contributed to our understanding of Helicobacter pylori biology and pathogenesis. However, the lack of convenient genetic tools, in particular the limited effectiveness of available reporter systems, has notably limited the toolbox for fundamental and applied studies. Here, we report the construction of a bioluminescent H. pylori reporter system based on the Photorhabdus luminescens luxCDABE cassette. The system is constituted of a promoterless lux acceptor strain in which promoters and sequences of interest can be conveniently introduced by double homologous recombination of a suicide transformation vector. We validate the robustness of this new lux reporter system in noninvasive in vivo monitoring of dynamic transcriptional responses of inducible as well as repressible promoters and demonstrate its suitability for the implementation of genetic screens in H. pylori.
authors: Department of Biology, University of Bologna, Bologna, Italy.
authors: Andrea
authors: A
authors: Vannini
authors: Francesca
authors: F
authors: Agriesti
authors: Flaviana
authors: F
authors: Mosca
authors: Davide
authors: D
authors: Roncarati
authors: Vincenzo
authors: V
authors: Scarlato
authors: Alberto
authors: A
authors: Danielli
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D017930:Genes, Reporter; D005822:Genetic Vectors; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D020637:Photorhabdus / Q000201:enzymology / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial