title: Analysis of protein-protein interactions in a complex environment: capture of an analyte-receptor complex with standard additions of the receptor (CARSAR) approach.
abstract: Traditional methods of analytical chemistry to detect an interaction between certain proteins in multicomponent mixtures (e.g. cell lysates, etc.) have limitations. This is due to difficulties in identification of a specific signal of an analyte (a molecule to be detected) against the background. In the present work, we propose the new analytical protocol for transducer-based sensors with a restricted sensitive area. It uses a combination of analyte-receptor complex precipitation with serial additions of the receptor (CARSAR). To test this new analytical strategy, we used a surface plasmon resonance technique to confirm an interaction between the Epstein-Barr virus-encoded nuclear antigen 6 and the mitochondrial ribosomal protein MRPS18-2.
authors: V. Lashkaryov Institute of Semiconductor Physics, NAS Ukraine, 41 Prospect Nauki, Kyiv, 03028, Ukraine.
authors: Boris A
authors: BA
authors: Snopok
authors: Suhas
authors: S
authors: Darekar
authors: Elena V
authors: EV
authors: Kashuba
mesh_terms: D019309:Epstein-Barr Virus Nuclear Antigens / Q000378:metabolism; D011485:Protein Binding; D012269:Ribosomal Proteins / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D019309:Epstein-Barr Virus Nuclear Antigens; D012269:Ribosomal Proteins