title: Experimental definition of a clustered regularly interspaced short palindromic duplicon in Escherichia coli.
abstract: We analyzed sequences of newly inserted repeats in an Escherichia coli CRISPR (clustered regularly interspaced short palindromic repeats) array in vivo and showed that a base previously thought to belong to the repeat is actually derived from a protospacer. Based on further experimental results, we propose to use the term "duplicon" for a repeated sequence in a CRISPR array that serves as a template for a new duplicon. Our findings suggest the possibility of redrawing the borders between repeats, spacers, and protospacer adjacent motifs.
authors: Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel.
authors: Moran G
authors: MG
authors: Goren
authors: Ido
authors: I
authors: Yosef
authors: Oren
authors: O
authors: Auster
authors: Udi
authors: U
authors: Qimron
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D055029:Inverted Repeat Sequences / Q000235:genetics*; D008969:Molecular Sequence Data; D056916:Segmental Duplications, Genomic / Q000235:genetics