title: Folate conjugated chitosan grafted thiazole orange derivative with high targeting for early breast cancer cells diagnosis.
abstract: The folate receptor (FR) is over-expressed on many solid tumors and has been exploited for targeted delivery of folic acid linked liposomes to cancer cells in vitro. In the present study, we developed a novel folic acid (FA) conjugated chitosan (CTS) grafted thiazole orange (TO) complex (FA-CTS-TO), and the formation can be used to label tumor cells. The structure of TO derivatives was confirmed by (1)H NMR and MS, and the fluorescence probe of FA-CTS-TO complex was confirmed by Fourier transform infrared analysis and Differential thermal analysis. The in vitro and in vivo of FA-CTS-TO complex were tested in breast cancer cells and the results showed a high targeting specificity in tumor cells with FR over-expressed. Such prominent fluorescence properties demonstrate again that FA-CTS-TO complex as a tumor targeting fluorescence probe is appropriate for breast cancer cells.
authors: Department of Materials Science and Engineering, Tianjin Institute of Urban Construction, Tianjin, China. xueningfei888@163.com
authors: Xue-Ning
authors: XN
authors: Fei
authors: Yin
authors: Y
authors: Liu
authors: Chao
authors: C
authors: Li
mesh_terms: D000588:Amines / Q000737:chemistry; D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000473:pathology*; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D015195:Drug Design; D055088:Early Detection of Cancer; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D058973:Folate Receptors, GPI-Anchored / Q000378:metabolism; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D011804:Quinolines / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013696:Temperature
chemical_list: D000588:Amines; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D058973:Folate Receptors, GPI-Anchored; D011804:Quinolines; C051000:thiazole orange; D012710:Serum Albumin, Bovine; D048271:Chitosan; D005492:Folic Acid