title: The translin-TRAX complex (C3PO) is a ribonuclease in tRNA processing.
abstract: Conserved translin-TRAX complexes, also known as C3POs, have been implicated in many biological processes, but how they function remains unclear. Recently, C3PO was shown to be an endoRNase that promotes RNA interference (RNAi) in animal cells. Here, we show that C3PO does not play a significant role in RNAi in the filamentous fungus Neurospora crassa. Instead, the Neurospora C3PO functions as an RNase that removes the 5' pre-tRNA fragments after the processing of pre-tRNAs by RNase P. In addition, translin and trax mutants have elevated levels of tRNA and protein translation and are more resistant to a cell death-inducing agent. Finally, we show that C3PO is also involved in tRNA processing in mouse embryonic fibroblast cells. This study identifies the endogenous RNA substrates of C3PO and provides a potential explanation for its roles in apparently diverse biological processes.
authors: Department of Physiology, The University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors: Liande
authors: L
authors: Li
authors: Weifeng
authors: W
authors: Gu
authors: Chunyang
authors: C
authors: Liang
authors: Qinghua
authors: Q
authors: Liu
authors: Craig C
authors: CC
authors: Mello
authors: Yi
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D004722:Endoribonucleases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques; D005800:Genes, Fungal; D051379:Mice; D057507:Mice, 129 Strain; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000378:metabolism; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010641:Phenotype; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D046912:Multiprotein Complexes; D012331:RNA, Fungal; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; C498303:Tsn protein, mouse; D012343:RNA, Transfer; D004722:Endoribonucleases