title: HER 2 status in invasive breast cancer: immunohistochemistry, fluorescence in-situ hybridization and chromogenic in-situ hybridization.
abstract: BACKGROUND
  HER2/neu gene status in breast cancers can be evaluated by targeting protein and gene - immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH). Recent studies have shown chromogenic in-situ hybridization (CISH) as a relatively cheaper alternative.
  
  
  METHODS
  Forty-three nonconsecutive, randomly selected primary invasive breast cancer cases were evaluated for c-erbB-2 (HER2 protein) by IHC and gene amplification by FISH and CISH. Results of each of the same were compared.
  
  
  RESULTS
  CISH showed approximately 90% and 100% concordance for IHC negative and positive cases, respectively; while approximately 94.4% and 91% concordance with FISH amplified and non-amplified cases, respectively.
  
  
  CONCLUSIONS
  This study showed feasibility of incorporation of CISH as a low cost option in routine management of breast carcinoma in the Indian setting. Secondly, reconfirmation of IHC negative and positive cases can be done by CISH.
authors: Division of Molecular Pathology, Department of Pathology, Tata Memorial Hospital and Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Mumbai, India.
authors: Hemlata S
authors: HS
authors: Shirsat
authors: Sridhar
authors: S
authors: Epari
authors: Tanuja
authors: T
authors: Shet
authors: Rajani
authors: R
authors: Bagal
authors: Rohini
authors: R
authors: Hawaldar
authors: Sangeeta B
authors: SB
authors: Desai
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000191:economics / Q000379:methods*; D017403:In Situ Hybridization / Q000191:economics / Q000379:methods*; D007194:India; D025202:Molecular Diagnostic Techniques / Q000191:economics / Q000379:methods; D010336:Pathology / Q000191:economics / Q000379:methods*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human