title: Nitric oxide is involved in the regulation of trehalose accumulation under heat stress in Pleurotus eryngii var. tuoliensis.
abstract: Little is known about the mechanism of how trehalose responds to various abiotic stresses although trehalose is considered as an important protectant in fungi. We investigated the role of nitric oxide (NO) in regulating trehalose accumulation during heat stress in Pleurotus eryngii var. tuoliensis. The addition of 100 or 200 g trehalose/l significantly inhibited the production of thiobarbituric acid-reactive substance under heat stress in mycelial cells. High temperature induced endogenous trehalose accumulation and sodium nitroprusside, a NO donor, further enhanced trehalose accumulation. Finally, heat-induced trehalose accumulation could be arrested by the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethyl-imidazoline-1-1-oxyl-3-oxide, at 250 μM by inhibiting the transcription of trehalose phosphate synthase gene. Thus NO plays an important role in the regulation of trehalose accumulation during abiotic stresses in P. eryngii var. tuoliensis.
authors: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Key Laboratory of Microbial Resources, Ministry of Agriculture, Beijing 100081, China.
authors: Wei-Wei
authors: WW
authors: Kong
authors: Chen-Yang
authors: CY
authors: Huang
authors: Qiang
authors: Q
authors: Chen
authors: Ya-Jie
authors: YJ
authors: Zou
authors: Meng-Ran
authors: MR
authors: Zhao
authors: Jin-Xia
authors: JX
authors: Zhang
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D006358:Hot Temperature; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D020076:Pleurotus / Q000378:metabolism*; D014199:Trehalose / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D009569:Nitric Oxide; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase