title: Genetic markers for SSG resistance in Leishmania donovani and SSG treatment failure in visceral leishmaniasis patients of the Indian subcontinent.
abstract: The current standard to assess pentavalent antimonial (SSG) susceptibility of Leishmania is a laborious in vitro assay of which the result has little clinical value because SSG-resistant parasites are also found in SSG-cured patients. Candidate genetic markers for clinically relevant SSG-resistant parasites identified by full genome sequencing were here validated on a larger set of clinical strains. We show that 3 genomic locations suffice to specifically detect the SSG-resistant parasites found only in patients experiencing SSG treatment failure. This finding allows the development of rapid assays to monitor the emergence and spread of clinically relevant SSG-resistant Leishmania parasites.
authors: Department of Biomedical Sciences, Institute of Tropical Medicine Antwerp, Belgium.
authors: Manu
authors: M
authors: Vanaerschot
authors: Saskia
authors: S
authors: Decuypere
authors: Tim
authors: T
authors: Downing
authors: Hideo
authors: H
authors: Imamura
authors: Olivia
authors: O
authors: Stark
authors: Simonne
authors: S
authors: De Doncker
authors: Syamal
authors: S
authors: Roy
authors: Bart
authors: B
authors: Ostyn
authors: Louis
authors: L
authors: Maes
authors: Basudha
authors: B
authors: Khanal
authors: Marleen
authors: M
authors: Boelaert
authors: Gabriele
authors: G
authors: Schönian
authors: Matthew
authors: M
authors: Berriman
authors: François
authors: F
authors: Chappuis
authors: Jean-Claude
authors: JC
authors: Dujardin
authors: Shyam
authors: S
authors: Sundar
authors: Suman
authors: S
authors: Rijal
mesh_terms: D000818:Animals; D000967:Antimony Sodium Gluconate / Q000627:therapeutic use*; D000981:Antiprotozoal Agents / Q000627:therapeutic use*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004351:Drug Resistance; D005819:Genetic Markers / Q000235:genetics; D018503:Genome, Protozoan; D006239:Haplotypes; D006801:Humans; D007194:India; D007893:Leishmania donovani / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000188:drug therapy* / Q000469:parasitology*; D051379:Mice; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D000981:Antiprotozoal Agents; D016054:DNA, Protozoan; D005819:Genetic Markers; D000967:Antimony Sodium Gluconate