title: A novel frog skin peptide containing function to induce muscle relaxation.
abstract: A novel bioactive peptide (polypedarelaxin 1) was identified from the skin secretions of the tree frog, Polypedates pingbianensis. Polypedarelaxin 1 is composed of 21 amino acid residues with a sequence of QGGLLGKVSNLANDALGILPI. Its primary structure was further confirmed by cDNA cloning and mass spectrometry analysis. Polypedarelaxin 1 was found to elicit concentration-dependent relaxation effects on isolated rat ileum. It has no antimicrobial and serine protease inhibitory activities. BLAST search revealed that polypedarelaxin 1 did not show similarity to known proteins or peptides. Especially, polypedarelaxin 1 do not contain conserved structural motifs of other amphibian myotropic peptides, such as bradykinins, bombesins, cholecystokinin (CCK), and tachykinins, indicating that polypedarelaxin 1 belongs to a novel family of amphibian myotropic peptide.
authors: Life Sciences College of Nanjing Agricultural University, 1st Weigang, Nanjing 210095, Jiangsu, China.
authors: Ping
authors: P
authors: Meng
authors: Lin
authors: L
authors: Wei
authors: Shilong
authors: S
authors: Yang
authors: Huan
authors: H
authors: Liu
authors: Rui
authors: R
authors: Liu
authors: Ren
authors: R
authors: Lai
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001001:Anura / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005260:Female; D007082:Ileum / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D008969:Molecular Sequence Data; D009126:Muscle Relaxation / Q000187:drug effects*; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000378:metabolism*
chemical_list: D029845:Amphibian Proteins; D018076:DNA, Complementary; D010455:Peptides; C578615:polypedarelaxin 1, Polypedates pingbianensis