title: A study of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) serum levels in rats subjected to fecal peritonitis and treated with intraperitoneal ropivacaine.
abstract: OBJECTIVE
  The objective of this study was to assess the cytokine serum levels of IL-6 and TNF-α in rats subjected to fecal peritonitis and treated with peritoneal lavage with 0.2% ropivacaine by peritoneal lavage.
  
  
  METHODS
  We subjected 16 Wistar rats to laparotomy 6 hours after the induction of fecal peritonitis with autogenous stool and subsequently divided the rats randomly into 4 groups: I-control, no treatment; II- drying of the abdominal cavity; III- lavage of the abdominal cavity with 3 mL of 0.9% normal saline and drying; IV- lavage of the abdominal cavity with 3 mL of 0.2% ropivacaine and drying. Six hours following the laparotomy, the animals underwent cardiac puncture, and 1 mL of blood was collected for cytokine assessment before the animals were euthanized.
  
  
  RESULTS
  The lavage with ropivacaine resulted in smaller TNF-α levels compared with those observed in the other treatment groups (p <0.05). Regarding IL-6, the ropivacaine group showed lower cytokine levels than those observed in groups I and II, but there was no significant difference (p> 0.05) between groups III and IV.
  
  
  CONCLUSIONS
  Peritoneal lavage with 0.2% ropivacaine was shown to reduce plasma levels of IL-6 and TNF-α in the treatment of fecal peritonitis in rats.
authors: Division of Anesthesiology, Department of Surgery, Espirito Santo Federal University, Brazil. mcbrocco@uol.com.br
authors: Marcos Célio
authors: MC
authors: Brocco
authors: Danilo Nagib Salomão
authors: DN
authors: Paulo
authors: Carlos Eduardo David de
authors: CE
authors: Almeida
authors: Antonio Roberto
authors: AR
authors: Carraretto
authors: Sigmar Aurea
authors: SA
authors: Cabral
authors: Amanda Cardoso de Oliveira
authors: AC
authors: Silveira
authors: Renato Santiago
authors: RS
authors: Gomez
authors: João Florêncio de Abreu
authors: JF
authors: Baptista
mesh_terms: D000577:Amides / Q000627:therapeutic use*; D000779:Anesthetics, Local / Q000627:therapeutic use*; D000818:Animals; D005243:Feces; D007274:Injections, Intraperitoneal; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D010533:Peritoneal Lavage / Q000009:adverse effects / Q000379:methods*; D010538:Peritonitis / Q000097:blood* / Q000188:drug therapy*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000077212:Ropivacaine; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D000577:Amides; D000779:Anesthetics, Local; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D000077212:Ropivacaine