title: Differences in adipose tissue inflammation and oxidative status in C57BL/6 and ApoE-/- mice fed high fat diet.
abstract: Apolipoprotein E deficient (Apo E-/-) mice are more resistant to the development of obesity compared to C57BL/6 wild type mice. They also hold a high basal oxidative status due to the loss of antioxidant action of apolipoprotein E. Since obesity is also an inducer of inflammation, we studied the effect of high-fat diet on obesity and oxidative stress in C57BL/6 and Apo E-/- mice for 9 weeks. The results confirmed that Apo E-/- mice fed high-fat diet are more resistant to the increase of both body weight and adiposity compared to C57BL/6 mice. Despite this, Apo E-/- mice presented a higher basal oxidative stress that was enhanced by high-fat diet. Macrophage infiltration, macrophage forming crown-like structures and proinflammatory adipokines (interleukin 6 and tumor necrosis factor alpha) were all higher in adipose tissue from Apo E-/- compared to C57BL/6 mice, regardless of diet type. In conclusion, although Apo E-/- mice are more resistant to becoming obese, they develop more severe adipose tissue inflammation companied by its consequences.
authors: Atherosclerosis and Nutritional Biochemistry Laboratory, Biochemistry and Immunology Department - Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. solangesilveirapereira@yahoo.com.br
authors: Solange S
authors: SS
authors: Pereira
authors: Lílian G
authors: LG
authors: Teixeira
authors: Edenil C
authors: EC
authors: Aguilar
authors: Rafael O
authors: RO
authors: Matoso
authors: Fabíola L P
authors: FL
authors: Soares
authors: Adaliene V M
authors: AV
authors: Ferreira
authors: Jacqueline I
authors: JI
authors: Alvarez-Leite
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency*; D059305:Diet, High-Fat / Q000009:adverse effects*; D007249:Inflammation / Q000209:etiology* / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology; D018384:Oxidative Stress / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001057:Apolipoproteins E; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha