title: Ni(2+)-modified gold nanoclusters for fluorescence turn-on detection of histidine in biological fluids.
abstract: In this work, Ni(2+)-modified gold nanoclusters were fabricated for fluorescence turn-on detection of histidine. The fluorescence of Au NCs was first quenched by Ni(2+). Then, the addition of histidine can restore the fluorescence of Au NCs by binding with Ni(2+) and removing it from the surface of the Au NCs. This architecture ensured non-toxic, cost-effective, label-free and sensitive detection of histidine. The developed Au NCs-based fluorescent sensor offered high selectivity for histidine over other amino acids. The relative standard deviation (RSD) for eleven replicate detections was 2.7%. The detection limit for histidine is 30 nM. The recovery of spiked histidine in human urine samples ranges from 95 to 104%.
authors: Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
authors: Yu
authors: Y
authors: He
authors: Xue
authors: X
authors: Wang
authors: Jingjing
authors: J
authors: Zhu
authors: Shuhua
authors: S
authors: Zhong
authors: Gongwu
authors: G
authors: Song
mesh_terms: D000818:Animals; D002417:Cattle; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006639:Histidine / Q000652:urine*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine; D006639:Histidine; D006046:Gold; D009532:Nickel