title: Impact of MCP-1 and CCR-2 gene polymorphisms on coronary artery disease susceptibility.
abstract: Coronary artery disease (CAD) was the second leading cause of death during the last 3 years in Taiwan. Smooth muscle cells, monocytes/macrophages, and endothelial cells produce monocyte chemoattractant protein-1 (MCP-1) within atherosclerotic plaques following binding to the chemokine receptor-2 (CCR-2). Previous studies have well-documented the association between MCP-1 expression and susceptibility to, or clinicopathological features, of CAD. This study investigated the relationships between MCP-1-2518A/G and CCR-2-V64I genetic polymorphisms and CAD in the Taiwanese population. A total of 608 subjects, including 392 non-CAD controls and 216 patients with CAD, were recruited and subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to evaluate the effects of these two polymorphic variants on CAD. Results indicated a significant association between MCP-1 -2548 gene polymorphism and susceptibility to CAD. GG genotypes (OR = 1.629; 95 % CI = 1.003-2.644), or individuals with at least one G allele (OR = 1.511; 95 % CI = 1.006-2.270), had a higher risk of CAD as compared with AA genotypes. Results also revealed that subjects with at least one A allele of the V64I CCR2 gene polymorphism had significantly increased risk of CAD. G allele in MCP-1-2518 might contribute to higher prevalence of atrial fibrillation in CAD patients (OR = 4.254; p < 0.05). In conclusion, MCP-1-2518G and CCR-2 64I gene polymorphisms represent important factors in determining susceptibility to CAD, and the contribution of MCP-1-2518G could be through effects on atrial fibrillation in CAD patients.
authors: Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan.
authors: Hsiu-Ling
authors: HL
authors: Lin
authors: Kwo-Chang
authors: KC
authors: Ueng
authors: Yih-Shou
authors: YS
authors: Hsieh
authors: Whei-Ling
authors: WL
authors: Chiang
authors: Shun-Fa
authors: SF
authors: Yang
authors: Shu-Chen
authors: SC
authors: Chu
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D018932:Chemokine CCL2 / Q000235:genetics*; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D054390:Receptors, CCR2 / Q000235:genetics*; D012307:Risk Factors
chemical_list: D018932:Chemokine CCL2; D054390:Receptors, CCR2