title: [Pharmacokinetics of nicotine in blood and brain using microdialysis and stable labelled isotope].
abstract: OBJECTIVE
  Using the stable isotopes as the internal standard of microdialysis technology to establish a new method to study the whole and local brain dynamics of nicotine percutaneous preparations.
  
  
  METHODS
  Using th healthy rats as experimental animals, administrating nicotine in abdominal transdermal way, then sample in the blood and brain simultaneously by microdialysis which use deuterium nicotine (DL-nicotine) as internal standard. Detecting the samples by LC-MS/MS method.
  
  
  RESULTS
  The configuration process in blood and brain both conforms to 2 compartments model, t1/2 is 29.38 min, t1/2beta is 208.51 min, AUC(0-infinity) is 152 127.10 microg x min x L(-1) in the blood t1/2 is 86.64 min, t1/2beta is 386.00 min, AUC(0-infinity) is 152 820.90 microg x min x L(-1) in the brain.
  
  
  CONCLUSIONS
  Dl-nicotine can be used as internal standard of nicotine to correcte the recovery; Stable isotopes internal standard microdialysis technology can be used for studing the whole and the local pharmacokinetic of nicotine and also provide new ideas and methods to studing the process of new drug delivery system.
authors: Department of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. piglion88@gmail.com
authors: Jiajun
authors: J
authors: Ling
authors: Xiujun
authors: X
authors: Wu
authors: Xiang
authors: X
authors: Fu
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D003903:Deuterium / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D017551:Microdialysis / Q000379:methods*; D009538:Nicotine / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009538:Nicotine; D003903:Deuterium