title: Extensive proteomic profiling of the secretome of European community acquired methicillin resistant Staphylococcus aureus clone.
abstract: European community acquired methicillin resistant Staphylococcus aureus (CA-MRSA) clone remains a striking pathogenic clone spreading in European and Mediterranean countries. Since analysis of the secretome produced from this clone by proteomics could provide a comprehensive picture of both core exoproteins as well as virulence factors, we applied two proteomic approaches, pre-fractionation of proteins on SDS-PAGE followed by in-gel trypsin digestion, and in-solution trypsin-digestion followed by off-line SCX fractionation, both of which were coupled with LC-MS/MS analyses. A total of 174 distinct proteins were identified with a high-confidence. Functional classification of these identified proteins resulted in16.09% of protein synthesis, 13.79% of virulence, 6.89% of toxin, and 17.24% of unknown function. Prediction of their cellular localizations revealed 18.39% in extracellular space, 36.20% in cytoplasm, 5.17% in cytoplasmic membranes, 6.89% in cell wall, 1.14% in multiple localizations, and 32.18% in unknown localization. Among them, 52% proteins were predicted to be secreted through signal peptide-independent pathways. Most notably, the expression of some proteins such as enterotoxins U and B were identified for the first time in this clone.
authors: Department of Structural Pathology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan. shymaa21@yahoo.com
authors: Shymaa
authors: S
authors: Enany
authors: Yutaka
authors: Y
authors: Yoshida
authors: Sameh
authors: S
authors: Magdeldin
authors: Ying
authors: Y
authors: Zhang
authors: Xu
authors: X
authors: Bo
authors: Tadashi
authors: T
authors: Yamamoto
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D017077:Culture Media, Conditioned / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D005060:Europe; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry; D059748:Proteolysis; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000382:microbiology*; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000737:chemistry; D037521:Virulence Factors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D004768:Enterotoxins; D010446:Peptide Fragments; D020543:Proteome; D037521:Virulence Factors; C034959:enterotoxin C, staphylococcal; D014357:Trypsin