title: Expression of interleukin-8 and intercellular cell adhesion molecule-1 in the synovial membrane and cranial cruciate ligament of dogs after rupture of the ligament.
abstract: This cross-sectional clinical study compared inflammation, including expression of the chemokine interleukin (IL)-8 and intercellular cell adhesion molecule-1 (ICAM-1), in the stifle joints of 4 control dogs and 23 dogs with cranial cruciate ligament rupture (CCLR). The CCL, synovial membrane, meniscus, cartilage, and synovial fluid from the affected stifle joints of all the dogs were examined. Inflammatory cell counts were performed on the synovial fluid, and the tissues were processed for histologic study and immunohistochemical detection of IL-8 and ICAM-1. The synovial fluid from the stifle joints of the dogs with CCLR had an increased percentage of neutrophils (P = 0.054) and a decreased percentage of lymphocytes (P = 0.004) but not macrophages compared with the fluid from the control dogs. There was accumulation of inflammatory cells and increased expression of IL-8 and ICAM-1 in the vascular endothelium of the synovial membrane and the CCL of the dogs with CCLR. The increase in inflammatory cells in the stifle joints of dogs with CCLR may therefore be due to increased expression of IL-8 and ICAM-1 in the synovial membrane and the CCL after the injury. These data may help in understanding the mechanisms of inflammation associated with CCLR.
authors: Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, 52 Campus Drive, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4, Canada.
authors: Mustafa
authors: M
authors: El-Hadi
authors: Chandarshekhar
authors: C
authors: Charavaryamath
authors: Andrea
authors: A
authors: Aebischer
authors: C Wayne
authors: CW
authors: Smith
authors: Cindy
authors: C
authors: Shmon
authors: Baljit
authors: B
authors: Singh
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D007150:Immunohistochemistry / Q000662:veterinary; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis*; D007958:Leukocyte Count / Q000662:veterinary; D012421:Rupture / Q000662:veterinary; D013264:Stifle / Q000378:metabolism* / Q000473:pathology; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology
chemical_list: D016209:Interleukin-8; D018799:Intercellular Adhesion Molecule-1