title: Immune role of MrNFκBI-α, an IκB family member characterized in prawn M. rosenbergii.
abstract: NF kappa B inhibitor alpha (MrNFκBI-α) was sequenced from a freshwater prawn Macrobrachium rosenbergii. The MrNFκBI-α protein contains a long ankyrin repeat region circular domain between 193 and 413 along with its 6 repeats (ankyrin repeat 1,2,3,4,5 and 6). An IκB degradation motif and a putative PEST motif is present at 37-64 and 418-471 of the N- and C-terminal regions of MrNFκBI-α respectively. The gene expressions of MrNFκBI-α in healthy and infectious hematopoietic and hypodermal necrosis virus (IHHNV), poly I:C, Aeromonas hydrophila and Enterococcus faecium injected M. rosenbergii were examined using quantitative real time PCR. The MrNFκBI-α is expressed in all the tissues taken for examination and the highest is observed in hemocytes. The MrNFκBI-α gene expression is strongly up-regulated in hemocytes of prawn after IHHNV, poly I:C, A. hydrophila and E. faecium infection. This result indicates an important role of MrNFκBI-α in M. rosenbergii immune system. This, however, remains to be verified by further studies.
authors: Department of Biotechnology, Faculty of Science and Humanities, SRM University, SRM Nagar, Kattankulathur 603203, Chennai, Tamil Nadu, India. jesuaraj@gmail.com
authors: Jesu
authors: J
authors: Arockiaraj
authors: Farhat Ahmadi
authors: FA
authors: Avin
authors: Puganeshwaran
authors: P
authors: Vanaraja
authors: Sarasvathi
authors: S
authors: Easwvaran
authors: Arun
authors: A
authors: Singh
authors: Rofina Yasmin
authors: RY
authors: Othman
authors: Subha
authors: S
authors: Bhassu
mesh_terms: D016980:Aeromonas hydrophila; D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics; D019223:Densovirinae / Q000502:physiology; D016984:Enterococcus faecium; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary; D016908:Gram-Positive Bacterial Infections / Q000276:immunology / Q000662:veterinary; D020672:I-kappa B Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D007274:Injections, Intraperitoneal / Q000662:veterinary; D008969:Molecular Sequence Data; D000072000:NF-KappaB Inhibitor alpha; D016366:Open Reading Frames; D009928:Organ Specificity; D033541:Palaemonidae / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D010322:Parvoviridae Infections / Q000276:immunology / Q000662:veterinary; D010802:Phylogeny; D011070:Poly I-C / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D020539:Sequence Analysis, Protein / Q000662:veterinary; D017386:Sequence Homology, Amino Acid
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D020672:I-kappa B Proteins; D012333:RNA, Messenger; D000072000:NF-KappaB Inhibitor alpha; D011070:Poly I-C