title: Isolation of ssDNA aptamers that inhibit rabies virus.
abstract: Aptamers, functional nucleic acids, capable of binding a variety of molecular targets with high affinity and specificity, have emerged as promising therapeutic agents. In this study, the cell surface-systematic evolution of ligands by exponential enrichment (Cell-SELEX) strategy was used to generate DNA aptamers which targeted to the intact rabies virus-infected live cells. Through 35 iterative rounds of selection, five high-affinity single-stranded DNA (ssDNA) aptamers were generated by cell-SELEX. Virus titer assay and real-time quantitative reverse transcription PCR (qRT-PCR) assay revealed that all five aptamers could inhibit replication of rabies virus (RABV) in cultured baby hamster kidney (BHK)-21 cells; and T14 and F34 aptamers were most effective. The qRT-PCR also showed a dose-dependent inhibitory effect in BHK-21 cells. Collectively, these data show the feasibility of generating functionally effective aptamers against rabies virus-infected cells by the Cell-SELEX iterative procedure. These aptamers may prove clinically useful as therapeutic molecules with specific antiviral potential against RABV infections.
authors: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors: Hong-Ru
authors: HR
authors: Liang
authors: Gui-Qiu
authors: GQ
authors: Hu
authors: Tao
authors: T
authors: Zhang
authors: Yu-Jiao
authors: YJ
authors: Yang
authors: Li-Li
authors: LL
authors: Zhao
authors: Ying-Lin
authors: YL
authors: Qi
authors: Hua-Lei
authors: HL
authors: Wang
authors: Yu-Wei
authors: YW
authors: Gao
authors: Song-Tao
authors: ST
authors: Yang
authors: Xian-Zhu
authors: XZ
authors: Xia
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D052157:Aptamers, Nucleotide / Q000494:pharmacology*; D002460:Cell Line; D006224:Cricetinae; D004277:DNA, Single-Stranded; D011820:Rabies virus / Q000187:drug effects* / Q000254:growth & development; D052156:SELEX Aptamer Technique
chemical_list: D000998:Antiviral Agents; D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded