title: [Establishment of an animal model of prostate cancer metastasis to the lung traced by AsRed2].
abstract: OBJECTIVE
  To establish an animal model of prostate cancer (PCa) metastasis to the lung using PCa PR7 (PCa PC-3 cells stably expressing red fluorescent protein AsRed2) cell lines that can be monitored by in vivo fluorescence imaging technology.
  
  
  METHODS
  MTT and Transwell assay were used to compare the abilities of proliferation, migration and invasion of PC-3 and PR7 cells. Twenty BALB/c nude mice were equally randomized to 4 groups to receive tail vein injection of PR7 cell suspension at the concentration of 1 x 107/ml (group A), 2.5 x 107/ml (group B), 5 x 107/ml (group C) and 2.5 x 107/ml followed by the same dose 1 week later (group D). PCa metastasis to the lung was then monitored by in vivo fluorescence imaging technology at the end of 2, 4, 6 and 8 weeks.
  
  
  RESULTS
  There were no statistically significant differences between PC-3 and PR7 cells in their abilities of proliferation, migration and invasion (P > 0.05). At the end of 4 weeks, lung metastasis was observed in 40% of the mice in group D, and at the end of 8 weeks, it was detected in 20% in group A, 60% in group B, 100% in group C, and 100% in group D, all confirmed by pathological examination.
  
  
  CONCLUSIONS
  The animal model of PCa metastasis to the lung that can be monitored by in vivo fluorescence imaging technology was established successfully by tail vein injection of PR7 cells carrying red fluorescent protein.
authors: Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China xjtulxd@163.com
authors: Xu-Dong
authors: XD
authors: Liu
authors: Jin
authors: J
authors: Zeng
authors: Shan
authors: S
authors: Xu
authors: Tao
authors: T
authors: Zhang
authors: Hong-Jun
authors: HJ
authors: Xie
authors: Zhen-Kun
authors: ZK
authors: Ma
authors: Xin-Yang
authors: XY
authors: Wang
authors: Da-Lin
authors: DL
authors: He
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D006801:Humans; D008164:Luminescent Proteins; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000556:secondary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D061848:Optical Imaging; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000473:pathology; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein