title: TxBR montage reconstruction for large field electron tomography.
abstract: Electron tomography (ET) has been proven an essential technique for imaging the structure of cells beyond the range of the light microscope down to the molecular level. Large-field high-resolution views of biological specimens span more than four orders of magnitude in spatial scale, and, as a consequence, are rather difficult to generate directly. Various techniques have been developed towards generating those views, from increasing the sensor array size to implementing serial sectioning and montaging. Datasets and reconstructions obtained by the latter techniques generate multiple three-dimensional (3D) reconstructions, that need to be combined together to provide all the multiscale information. In this work, we show how to implement montages within TxBR, a tomographic reconstruction software package. This work involves some new application of mathematical concepts related to volume preserving transformations and issues of gauge ambiguity, which are essential problems arising from the nature of the observation in an electron microscope. The purpose of TxBR is to handle those issues as generally as possible in order to correct for most distortions in the 3D reconstructions and allow for a seamless recombination of ET montages.
authors: National Center For Microscopy and Imaging Research, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0608, USA.
authors: Sébastien
authors: S
authors: Phan
authors: Albert
authors: A
authors: Lawrence
authors: Tomas
authors: T
authors: Molina
authors: Jason
authors: J
authors: Lanman
authors: Monica
authors: M
authors: Berlanga
authors: Masako
authors: M
authors: Terada
authors: Alexander
authors: A
authors: Kulungowski
authors: James
authors: J
authors: Obayashi
authors: Mark
authors: M
authors: Ellisman
mesh_terms: D000465:Algorithms; D000818:Animals; D001921:Brain / Q000166:cytology; D004330:Drosophila / Q000166:cytology / Q000821:virology; D055032:Electron Microscope Tomography / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007304:Insect Viruses / Q000502:physiology; D051379:Mice; D009474:Neurons / Q000648:ultrastructure; D012984:Software