title: Transcutaneous DNA immunization following waxing-based hair depilation elicits both humoral and cellular immune responses.
abstract: Previously, we showed that transcutaneous (TC) DNA immunization by applying plasmid DNA onto a mouse skin area wherein the hair follicles were induced into growth stage by plucking the hair using warm waxing induced strong and functional antigen-specific antibody responses. In the present study, using plasmids that encode β-galactosidase gene or ovalbumin (OVA) gene, we showed that this mode of TC DNA immunization not only induced specific antibody responses, but also induced antigen-specific cytotoxic T lymphocyte responses. In fact, TC DNA immunization using a plasmid that encodes OVA gene prevented the growth of OVA-expressing B16-OVA tumor cells in the immunized mice. Moreover, we provided additional evidence supporting that hair follicles are essential for this mode of TC DNA immunization.
authors: The University of Texas at Austin, College of Pharmacy, Austin, TX 78723, USA.
authors: Gang
authors: G
authors: Xiao
authors: Xinran
authors: X
authors: Li
authors: Amit
authors: A
authors: Kumar
authors: Zhengrong
authors: Z
authors: Cui
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000941:Antigens / Q000276:immunology; D005260:Female; D006204:Hair Removal / Q000379:methods*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007114:Immunization / Q000379:methods; D008546:Melanoma, Experimental / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000235:genetics; D010957:Plasmids; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology / Q000493:pharmacokinetics; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D000941:Antigens; D019444:Vaccines, DNA; D010047:Ovalbumin; D001616:beta-Galactosidase