title: Identification of a newly isolated erythritol-producing yeast and cloning of its erythrose [corrected] reductase genes.
abstract: A new erythritol-producing yeast (strain BH010) was isolated in this study. Analysis of the D1/D2 domain of the 26S rDNA sequence, the ITS/5.8S rDNA sequence [corrected] and the 18S rDNA sequence allowed the taxonomic position of strain BH010 to be discussed, [corrected] and it was identified and named Moniliella sp. BH010. Physiological characteristics were described. Scanning electron micrography clearly indicated that the cells were cylindrical to elliptical with an average size of 5 × 10 μm when growing in liquid medium [corrected] and that pseudohyphae and blastoconidia were observed when cultivated in agar plates. The erythrose [corrected] reductase genes were cloned, sequenced, and analyzed. BLAST analysis and multiple sequence alignment demonstrated that erythrose [corrected] reductase genes of Moniliella sp. BH010 shared very high homology with that of Trichosporonoides megachiliensis SNG-42 except for the presence of introns. The deduced amino acid sequences showed high homology to the aldo-keto reductase superfamily.
authors: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Huihui
authors: H
authors: Deng
authors: Ye
authors: Y
authors: Han
authors: Yuanyuan
authors: Y
authors: Liu
authors: Wei
authors: W
authors: Jia
authors: Zhijiang
authors: Z
authors: Zhou
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics; D004896:Erythritol / Q000096:biosynthesis* / Q000378:metabolism; D007438:Introns; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D004275:DNA, Ribosomal; D010088:Oxidoreductases; D004896:Erythritol