title: Production of Chitinase and its Optimization from a Novel Isolate Serratia marcescens XJ-01.
abstract: Production of chitinase from bacteria has distinct advantages over fungi, due to the formation of mycelia of fungi in the later phase of fermentation. A novel chitinase-producing bacterial strain XJ-01 was isolated from the Yulu fishing field of Changsha, Hunan province, China, by enrichment and spread-plate technique, sequentially. Physicochemical characterization and 16S rRNA sequencing revealed that strain XJ-01 belongs to Serratia marcescens. By optimizing the fermentation condition based on L(9)(3(4)) orthogonal experimental design, a maximal chitinase activity up to 15.36 U/ml was attained by that stain under the condition: 0.5% (NH(4))(2)SO(4) as the nitrogen source, 0.75% colloidal chitin as the carbon source, temperature of 32°C, time of 32 h and pH 8.0.
authors: Jin-Lan
authors: JL
authors: Xia
authors: Jing
authors: J
authors: Xiong
authors: Rui-Yong
authors: RY
authors: Zhang
authors: Ke-Ke
authors: KK
authors: Liu
authors: Bin
authors: B
authors: Huang
authors: Zhen-Yuan
authors: ZY
authors: Nie