title: Fiber-substituted conditionally replicating adenovirus for oncolysis of human renal carcinoma cells.
abstract: BACKGROUND
  Adenovirus vectors have lately been highlighted in gene therapies. We investigated the oncolytic effects of a chimeric adenovirus type 5 (Ad5) with replacement of Ad5 fiber knob with adenovirus type 35 (Ad35) fiber knob (Ad5F35) on human renal cell carcinoma (RCC).
  
  
  METHODS
  The conditionally replicating Ad5F35 vector was constructed and infected into RCC cell lines 786-O, ACHN, and RCC4-VHL. For these cells, reverse transcription-polymerase chain reaction and western blotting were carried out and the cell viability was assayed.
  
  
  RESULTS
  In all RCC cell lines, it was found that CD46, a cell surface target of Ad35, was well-expressed, while coxsackie and adenovirus receptor (CAR), a cell surface target of Ad5, was considerably less expressed. The Ad5F35 vector induced oncolysis of RCC cells, with significantly higher efficacy as compared with that for the Ad5 vector.
  
  
  CONCLUSIONS
  Ad5F35 vector could be a candidate for promising gene therapy of human RCC.
authors: Laboratory of Cell and Gene Therapy, Institute for Advanced Medical Sciences, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya 663-8501, Japan.
authors: Hisao
authors: H
authors: Nagaya
authors: Masatoshi
authors: M
authors: Tagawa
authors: Kazuhiro
authors: K
authors: Hiwasa
authors: Shuji
authors: S
authors: Terao
authors: Takeshi
authors: T
authors: Kanno
authors: Tomoyuki
authors: T
authors: Nishizaki
authors: Akinobu
authors: A
authors: Gotoh
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000502:physiology*; D017498:Adenovirus E1 Proteins / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000628:therapy* / Q000821:virology*; D045744:Cell Line, Tumor; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D016207:Cytokines / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D007680:Kidney Neoplasms / Q000628:therapy* / Q000821:virology*; D051916:Membrane Cofactor Protein / Q000096:biosynthesis / Q000235:genetics; D000078792:Midkine; D050130:Oncolytic Virotherapy / Q000379:methods*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011991:Receptors, Virus / Q000096:biosynthesis / Q000235:genetics; D014779:Virus Replication
chemical_list: D017498:Adenovirus E1 Proteins; C497857:CD46 protein, human; C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D016207:Cytokines; D051916:Membrane Cofactor Protein; D012333:RNA, Messenger; D011991:Receptors, Virus; D000078792:Midkine