title: Genetic diversity of dengue virus serotypes 1 and 2 in the State of Paraná, Brazil, based on a fragment of the capsid/premembrane junction region.
abstract: BACKGROUND
  The precise identification of the genetic variants of the dengue virus is important to understand its dispersion and virulence patterns and to identify the strains responsible for epidemic outbreaks. This study investigated the genetic variants of the capsid-premembrane junction region fragment in the dengue virus serotypes 1 and 2 (DENV1-2).
  
  
  METHODS
  Samples from 11 municipalities in the State of Paraná, Brazil, were provided by the Central Laboratory of Paraná. They were isolated from the cell culture line C6/36 (Aedes albopictus) and were positive for indirect immunofluorescence. Ribonucleic acid (RNA) extracted from these samples was submitted to the reverse transcription polymerase chain reaction (RT-PCR) and nested PCR.
  
  
  RESULTS
  RT-PCR revealed that 4 of the samples were co-infected with both serotypes. The isolated DENV-1 sequences were 95-100% similar to the sequences of other serotype 1 strains deposited in GenBank. Similarly, the isolated DENV-2 sequences were 98-100% similar to other serotype 2 sequences in GenBank. According to our neighbor-joining tree, all strains obtained in this study belonged to genotype V of DENV-1. The DENV-2 strains, by contrast, belonged to the American/Asian genotypes.
  
  
  CONCLUSIONS
  The monitoring of circulating strains is an important tool to detect the migration of virus subtypes involved in dengue epidemics.
authors: Laboratório de Entomologia Médica e Veterinária, Setor de Ciências Biológicas, Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, PR, Brasil.
authors: Ana Caroline Dalla
authors: AC
authors: Bona
authors: Adriana Lacerda
authors: AL
authors: Twerdochlib
authors: Mário Antônio
authors: MA
authors: Navarro-Silva
mesh_terms: D000330:Aedes / Q000821:virology; D000818:Animals; D001938:Brazil; D036022:Capsid Proteins / Q000235:genetics*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019084:Fluorescent Antibody Technique, Indirect; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral