title: Improvement in hemocompatibility of chitosan/soy protein composite membranes by heparinization.
abstract: OBJECTIVE
  To improve the hemocompatibility of chitosan/soy protein isolate composite membranes by heparinization.
  
  
  METHODS
  Chitosan/soy protein isolate membranes (ChS-n, n=0, 10 and 30, corresponding to the soy protein isolate content in the membranes) and heparinized ChS-n membranes (HChS-n) were prepared by blending in dilute HAc/NaAc solution. The hemocompatibility of ChS-n and HChS-n membranes were comparatively evaluated by measuring surface heparin density, blood platelet adhesion, plasma recalcification time (PRT), thrombus formation and hemolysis assay.
  
  
  RESULTS
  The surface heparin density analysis showed that heparinized chitosan/SPI soy protein isolate membranes have been successfully prepared by blending. The density of heparin on the surface of HChS-n membranes was in the range of 0.67-1.29 μg/cm2. The results of platelet adhesion measurement showed that the platelet adhesion numbers of HChS-n membranes were lower than those of the corresponding ChS-n membranes. The PRT of the HChS-0, HChS-10 and HChS-30 membranes were around 292, 306 and 295 s, respectively, which were longer than the corresponding ChS-0 (152 s), ChS-10 (204 s) and ChS-30 (273 s) membranes. The hemolysis rate of HChS-n membranes was lower than 1%.
  
  
  CONCLUSIONS
  The hemocompatibility of ChS membranes could be improved by blending with heparin. Compared with ChS membranes, HChS membranes showed lower platelet adhesion, longer PRT, higher BCI, significant thromboresistivity and a lower hemolysis rate due to the heparinization. This widens the application of chitosan and soy protein-based biomaterials that may come in contact with blood.
authors: Department of Biomedical Engineering, School of Basic Medical Science, Wuhan University, Wuhan, China.
authors: Xiaomei
authors: X
authors: Wang
authors: Na
authors: N
authors: Shi
authors: Yan
authors: Y
authors: Chen
authors: Chen
authors: C
authors: Li
authors: Xinshen
authors: X
authors: Du
authors: Weihua
authors: W
authors: Jin
authors: Yun
authors: Y
authors: Chen
authors: Peter R
authors: PR
authors: Chang
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000378:metabolism; D001777:Blood Coagulation; D001792:Blood Platelets / Q000166:cytology; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology; D006461:Hemolysis; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism; D008422:Materials Testing; D008567:Membranes, Artificial; D010973:Platelet Adhesiveness; D011817:Rabbits; D030262:Soybean Proteins / Q000737:chemistry* / Q000378:metabolism; D013927:Thrombosis / Q000209:etiology
chemical_list: D000925:Anticoagulants; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D030262:Soybean Proteins; D006493:Heparin; D048271:Chitosan