title: Comparative proteomic profiling of dystroglycan-associated proteins in wild type, mdx, and Galgt2 transgenic mouse skeletal muscle.
abstract: Dystroglycan is a major cell surface glycoprotein receptor for the extracellular matrix in skeletal muscle. Defects in dystroglycan glycosylation cause muscular dystrophy and alterations in dystroglycan glycosylation can impact extracellular matrix binding. Here we describe an immunoprecipitation technique that allows isolation of beta dystroglycan with members of the dystrophin-associated protein complex (DAPC) from detergent-solubilized skeletal muscle. Immunoprecipitation, coupled with shotgun proteomics, has allowed us to identify new dystroglycan-associated proteins and define changed associations that occur within the DAPC in dystrophic skeletal muscles. In addition, we describe changes that result from overexpression of Galgt2, a normally synaptic muscle glycosyltransferase that can modify alpha dystroglycan and inhibit the development of muscular dystrophy when it is overexpressed. These studies identify new dystroglycan-associated proteins that may participate in dystroglycan's roles, both positive and negative, in muscular dystrophy.
authors: Center for Gene Therapy, The Research Institute at Nationwide Children's Hospital, The Ohio State University, Columbus, Ohio 43205, USA.
authors: Jung Hae
authors: JH
authors: Yoon
authors: Eric
authors: E
authors: Johnson
authors: Rui
authors: R
authors: Xu
authors: Laura T
authors: LT
authors: Martin
authors: Paul T
authors: PT
authors: Martin
authors: Federica
authors: F
authors: Montanaro
mesh_terms: D000818:Animals; D015153:Blotting, Western; D049030:Dystroglycans / Q000737:chemistry / Q000378:metabolism*; D016189:Dystrophin / Q000235:genetics* / Q000378:metabolism; D049028:Dystrophin-Associated Protein Complex / Q000737:chemistry / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D047468:Immunoprecipitation; D019409:Interleukin-15 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D008840:Microfilament Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D009136:Muscular Dystrophies / Q000235:genetics / Q000378:metabolism; D025941:Protein Interaction Mapping; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D016189:Dystrophin; D049028:Dystrophin-Associated Protein Complex; D019409:Interleukin-15; D008840:Microfilament Proteins; D020543:Proteome; C556674:Spir-2 protein, mouse; D049030:Dystroglycans; D016695:Glycosyltransferases; C505903:Galgt2 protein, mouse