title: In vivo antitumor effect of liposomes with sialyl Lewis X including             monogalactosyl diacylglycerol, a replicative DNA polymerase inhibitor, from spinach.
abstract: The glycoglycerolipid monogalactosyl diacylglycerol (MGDG) isolated from             spinach selectively inhibits the activities of replicative DNA polymerase species             and suppresses the growth of human cancer cell lines, while not affecting normal             human cells. Liposomes, carrying surface-bound sialyl Lewis X (SLX) and containing             MGDG (SLX-Lipo-MGDG) and the fluorescent dye Cy5.5, were administered intravenously             to mice bearing HT-29 human colon adenocarcinoma tumors and liposome distribution             observed using fluorescence imaging equipment in vivo. In an in vivo antitumor             assay on nude mice bearing HT-29 solid tumors, SLX-Lipo-MGDG was shown to be a             stronger and more promising suppressor of solid tumors than MGDG alone. These             results suggest that spinach MGDG could be developed into an anticancer compound,             SLX-Lipo-MGDG could serve as an effective clinical anticancer drug and that these             liposomes may be useful tools as the basis for active targeting drug delivery             systems.
authors: Department of Nutritional Science, Laboratory of Food and Nutritional Sciences, Nishi-ku, Kobe, Hyogo 651-2180, Japan. mizushin@nutr.kobegakuin.ac.jp
authors: Yoshiyuki
authors: Y
authors: Mizushina
authors: Takahiko
authors: T
authors: Hada
authors: Hiromi
authors: H
authors: Yoshida
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D005260:Female; D038983:Galactolipids / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D019073:HT29 Cells; D006801:Humans; D008081:Liposomes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D019384:Nucleic Acid Synthesis Inhibitors; D009844:Oligosaccharides / Q000008:administration & dosage*; D010316:Particle Size; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D000080506:Sialyl Lewis X Antigen; D018724:Spinacia oleracea / Q000737:chemistry; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D038983:Galactolipids; D008081:Liposomes; D019384:Nucleic Acid Synthesis Inhibitors; D009844:Oligosaccharides; D010936:Plant Extracts; D000080506:Sialyl Lewis X Antigen; C009909:monogalactosyldiacylglycerol; D004259:DNA-Directed DNA Polymerase