title: Bioremediation of waste cooking oil using a novel lipase produced by Penicillium chrysogenum SNP5 grown in solid medium containing waste grease.
abstract: The aim of present work was to bioremediate the waste cooking oil using a novel lipase produced in solid medium containing waste grease and wheat bran by Penicillium chrysogenum. Enzyme extracted with phosphate buffer was purified 10.6 and 26.28-fold after 90% ammonium sulfate precipitation and ion-exchange chromatography, respectively. The partial characterization of enzyme revealed its K(m) and V(max) value for p-nitrophenolpamitate as 0.4mM and 47.61 U/ml, respectively. The relative molecular mass of lipase was 40 kDa by SDS-PAGE and confirmed by zymogram. Purified lipase was most stable at 40°C and at 8.0 pH. Lipase activity was enhanced by metal ions such as Mg(2+), Fe(2+), Ca(2+) and non-ionic surfactant TritonX-100, while suppressed in the presence of SDS. Crude lipase was applied on cooking oil waste and the acid value was 26.92 mg/g. This showed that the enzyme could be employed for the bioremediation of used cooking oil.
authors: Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad 211 004, Uttar Pradesh, India.
authors: Sunil
authors: S
authors: Kumar
authors: Anisha
authors: A
authors: Mathur
authors: Varsha
authors: V
authors: Singh
authors: Suchismita
authors: S
authors: Nandy
authors: Sunil Kumar
authors: SK
authors: Khare
authors: Sangeeta
authors: S
authors: Negi
mesh_terms: D000143:Acids; D001673:Biodegradation, Environmental / Q000187:drug effects; D002849:Chromatography, Gas; D003296:Cooking; D003470:Culture Media / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability / Q000187:drug effects; D008049:Lipase / Q000096:biosynthesis*; D009821:Oils / Q000378:metabolism*; D010408:Penicillium chrysogenum / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development* / Q000302:isolation & purification; D013501:Surface-Active Agents / Q000494:pharmacology; D014866:Waste Products / Q000032:analysis*
chemical_list: D000143:Acids; D003470:Culture Media; D009821:Oils; D013501:Surface-Active Agents; D014866:Waste Products; D008049:Lipase