title: The interaction between viral protein and host actin facilitates the virus infection to host.
abstract: Although the virus-host interaction has attracted extensive studies, the host proteins essential for virus infection remain largely unknown. To address this issue, the shrimp Penaeus stylirostris densovirus (PstDNV), belonging to the family Parvoviridae, was characterized. PstDNV, a single-stranded DNA virus with a 3.9-kb genome, encoded only three open reading frames (ORFs). Among the three viral proteins, the PstDNV ORF2-encoded protein was discovered to interact with the shrimp actin, suggesting that the host actin played a very important role in virus infection. The RNAi assays revealed that the ORF2-encoded protein was required for the PstDNV infection. The confocal evidence demonstrated that the interaction between the ORF2-encoded protein and actin was essential for the virus infection. Therefore our study indicated that the manipulation of the host actin cytoskeleton was a necessary strategy for viral pathogens to invade host cells.
authors: Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Geng
authors: G
authors: Yang
authors: Xiao
authors: X
authors: Xiao
authors: Dongsheng
authors: D
authors: Yin
authors: Xiaobo
authors: X
authors: Zhang
mesh_terms: D000199:Actins / Q000502:physiology*; D000818:Animals; D060829:Arthropod Proteins / Q000502:physiology; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D017990:Densovirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D005814:Genes, Viral; D006434:Hemocytes / Q000502:physiology / Q000821:virology; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000502:physiology*; D033561:Penaeidae / Q000502:physiology / Q000821:virology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*
chemical_list: D000199:Actins; D060829:Arthropod Proteins; D004279:DNA, Viral; D034741:RNA, Small Interfering; D014764:Viral Proteins