title: Expression of miRNAs and their cooperative regulation of the pathophysiology in traumatic brain injury.
abstract: Traumatic brain injury (TBI) is a leading cause of injury-related death and disability worldwide. Effective treatment for TBI is limited and many TBI patients suffer from neuropsychiatric sequelae. The molecular and cellular mechanisms underlying the neuronal damage and impairment of mental abilities following TBI are largely unknown. Here we used the next generation sequencing platform to delineate miRNA transcriptome changes in the hippocampus at 24 hours and 7 days following TBI in the rat controlled cortical impact injury (CCI) model, and developed a bioinformatic analysis to identify cellular activities that are regulated by miRNAs differentially expressed in the CCI brains. The results of our study indicate that distinct sets of miRNAs are regulated at different post-traumatic times, and suggest that multiple miRNA species cooperatively regulate cellular pathways for the pathological changes and management of brain injury. The distinctive miRNAs expression profiles at different post-CCI times may be used as molecular signatures to assess TBI progression. In addition to known pathophysiological changes, our study identifies many other cellular pathways that are subjected to modification by differentially expressed miRNAs in TBI brains. These pathways can potentially be targeted for development of novel TBI treatment.
authors: Unit on Synapse Development and Plasticity, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, United States of America.
authors: Zhonghua
authors: Z
authors: Hu
authors: Danni
authors: D
authors: Yu
authors: Camila
authors: C
authors: Almeida-Suhett
authors: Kang
authors: K
authors: Tu
authors: Ann M
authors: AM
authors: Marini
authors: Lee
authors: L
authors: Eiden
authors: Maria F
authors: MF
authors: Braga
authors: Jun
authors: J
authors: Zhu
authors: Zheng
authors: Z
authors: Li
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D006624:Hippocampus / Q000293:injuries* / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D059467:Transcriptome
chemical_list: D035683:MicroRNAs