title: A monoclonal antibody against the catalytic domain of PTP1B.
abstract: Protein tyrosine phosphatase 1B (PTP1B), a member of the protein tyrosine phosphatase (PTP) family, plays a crucial role in metabolic signaling, with insulin and leptin signaling being well studied. New evidence indicates that PTP1B is also involved in cancer. In the present study, we report on the establishment of a monoclonal antibody specific for catalytic domain of PTP1B (PTP1Bc) generated through the hybridoma method. The monoclonal antibody is measured to have a titer of 4.1×10(6) against PTP1Bc in indirect ELISA. Western blot and immunofluorescent analyses indicated that this antibody can specifically combine native PTP1B in MDA-MB-231 and MDA-MB-453 cells. This monoclonal antibody against PTP1Bc can help enhance the understanding of PTP1B-related physiological and pathological mechanisms and may act as a therapeutic agent for diabetes, obesity, and cancer in the future.
authors: Cancer Research Center, Medical College, Xiamen University, Xiamen, Fujian, China.
authors: Fanwei
authors: F
authors: Zeng
authors: Erru
authors: E
authors: Ni
authors: Shengyu
authors: S
authors: Wang
authors: Sha
authors: S
authors: Lv
authors: Chang
authors: C
authors: Cao
authors: Haipeng
authors: H
authors: Zhang
authors: Haiyan
authors: H
authors: Lv
authors: Xiaoli
authors: X
authors: Chen
authors: Jianghua
authors: J
authors: Yan
authors: Fanghong
authors: F
authors: Luo
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D020134:Catalytic Domain / Q000276:immunology; D045744:Cell Line, Tumor; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D006825:Hybridomas; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D017186:Titrimetry
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; C516607:PTPN1 protein, human; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1