title: A prospective assessment of the accuracy of commercial IgM ELISAs in diagnosis of Japanese encephalitis virus infections in patients with suspected central nervous system infections in Laos.
abstract: Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia. We estimated the diagnostic accuracy of two anti-JEV immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) (Panbio and XCyton JEVCheX) compared with a reference standard (AFRIMS JEV MAC ELISA) in a prospective study of the causes of central nervous system infections in Laos. Cerebrospinal fluid (CSF; 515 patients) and serum samples (182 patients) from those admitted to Mahosot Hospital, Vientiane, were tested. The CSF from 14.5% of acute encephalitis syndrome (AES) patients and 10.1% from those with AES and meningitis were positive for anti-JEV IgM in the reference ELISA. The sensitivities for CSF were 65.4% (95% confidence interval [CI] = 51-78) (Xcyton), 69.2% (95% CI = 55-81) (Panbio), however 96.2% (95% CI = 87-100) with Panbio Ravi criteria. Specificities were 89-100%. For admission sera from AES patients, sensitivities and specificities of the Panbio ELISA were 85.7% (95% CI = 42-100%) and 92.9% (95% CI = 83-98%), respectively.
authors: Catrin E
authors: CE
authors: Moore
authors: Stuart D
authors: SD
authors: Blacksell
authors: Thaksinaporn
authors: T
authors: Taojaikong
authors: Richard G
authors: RG
authors: Jarman
authors: Robert V
authors: RV
authors: Gibbons
authors: Sue J
authors: SJ
authors: Lee
authors: Vilada
authors: V
authors: Chansamouth
authors: Soulignasack
authors: S
authors: Thongpaseuth
authors: Mayfong
authors: M
authors: Mayxay
authors: Paul N
authors: PN
authors: Newton
mesh_terms: D000293:Adolescent; D000328:Adult; D002494:Central Nervous System Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D002648:Child; D004672:Encephalitis, Japanese / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D007812:Laos; D008297:Male; D011446:Prospective Studies
chemical_list: D007075:Immunoglobulin M