title: Headgroup specificity for the interaction of the antimicrobial peptide tritrpticin with phospholipid Langmuir monolayers.
abstract: We examined the interaction of the cationic antimicrobial peptide (AMP) tritrpticin (VRRFPWWWPFLRR, TRP3) with Langmuir monolayers of zwitterionic (dipalmitoyl phosphatidylcholine, DPPC, and dipalmitoyl phosphatidylethanolamine, DPPE) and negatively charged phospholipids (dipalmitoyl phosphatidic acid, DPPA, and dipalmitoyl phosphatidylglycerol, DPPG). Both surface pressure and surface potential isotherms became more expanded upon addition of TRP3 (DPPE~DPPC<<DPPA<DPPG). The stronger interaction with negatively charged phospholipids agrees with data for vesicles and planar lipid bilayers, and with AMPs greater activity against bacterial membranes versus mammalian cell membranes. Considerable expansion of negatively charged monolayers occurred at 10 and 30 mol% TRP3, especially at low surface pressure. Moreover, a difference was observed between PA and PG, demonstrating that the interaction, besides being modulated by electrostatic interactions, displays specificity with regard to headgroup, being more pronounced in the case of PG, present in large quantities in bacterial membranes. In previous studies, it was proposed that the peptide acts by a toroidal pore-like mechanism. Considering the evidence from the literature that PG shows a propensity to form a positive curvature as do toroidal pores, the observation of TRP3's preference for the PG headgroup and the dramatic increase in area promoted by this interaction represent further support for the toroidal pore mechanism of action proposed for TRP3.
authors: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP, Brazil.
authors: Luiz C
authors: LC
authors: Salay
authors: Marystela
authors: M
authors: Ferreira
authors: Osvaldo N
authors: ON
authors: Oliveira
authors: Clovis R
authors: CR
authors: Nakaie
authors: Shirley
authors: S
authors: Schreier
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D001419:Bacteria / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry; D005057:Eukaryotic Cells / Q000737:chemistry; D008567:Membranes, Artificial; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D010712:Phosphatidic Acids / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D008567:Membranes, Artificial; D009842:Oligopeptides; D010712:Phosphatidic Acids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; C402895:tritrpticin; C031599:dipalmitoylphosphatidic acid; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; C030345:1,2-dipalmitoylphosphatidylglycerol