title: Effects of Helicobacter pylori on biological characteristics of gastric epithelial cells.
abstract: Infection with Helicobacter pylori (H. pylori) strains is linked to an increased risk of inflammation and gastric cancer. To investigate the effects of H. pylori on biological characteristics of gastric epithelial cells SGC-7901, derived from human adenocarcinoma, morphological appearances of both the pathogen and these cells, as well as features of attachment and internalization were observed by using transmission electron microscopy (TEM). We also investigated cell junctions and invasion by TEM and Transwell Invasion Assay. Cell proliferation and apoptosis were assessed by using chromogenic methylthiazol tetrazolium bromide (MTT) dye and flow cytometry. Three types of H. pylori were observed around, attaching to, or invading tumor cells. Cellular damage was characterized by vacuolar degeneration, dilated endoplasmic reticulum (ER), and reduction of organelles. Cell junctions and cell microvilli reduced or disappeared. H. pylori inhibited cell proliferation, whereas it had no effect on apoptosis. It also promoted gastric carcinoma cell invasion. H. pylori damages cell construction, destroys cell junctions, inhibits cell proliferation, promotes cell invasive ability, and, therefore, might accelerate the malignant progress and metastasis of gastric cancer.
authors: Department of Pathology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.
authors: Ping
authors: P
authors: Wang
authors: Juan
authors: J
authors: Mei
authors: Jing
authors: J
authors: Tao
authors: Ning
authors: N
authors: Zhang
authors: Hua
authors: H
authors: Tian
authors: Guo-Hui
authors: GH
authors: Fu
mesh_terms: D017209:Apoptosis; D001422:Bacterial Adhesion / Q000502:physiology; D015820:Cadherins / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D004848:Epithelium / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology*; D005753:Gastric Mucosa / Q000378:metabolism; D016481:Helicobacter Infections / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000472:pathogenicity* / Q000648:ultrastructure; D054884:Host-Pathogen Interactions; D006801:Humans; D007365:Intercellular Junctions / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D015388:Organelles / Q000648:ultrastructure; D013270:Stomach / Q000382:microbiology* / Q000473:pathology*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000382:microbiology / Q000473:pathology
chemical_list: D015820:Cadherins