title: [Isolation, identification and characterization of SFTS bunyavirus from ticks collected on the surface of domestic animals].
abstract: To understand the maintenance and transmission of SFTS virus, the potential vector ticks were collected from sheep, cattle and dogs in the endemic areas of SFTSV in Shandong Province. Among the collected ticks, the dominant species was H. longicornis ticks. Real-time PCR for RNA detection, virus isolation and characterization, genomic sequencing, phylogenetic and antigenic analysis were performed in this investigation. The results showed that the SFTS viral RNA was detected in 2.14% H. longicornis, and a SFTS virus was isolated from one of viral RNA positive ticks collected from sheep. Whole genome analysis of the SFTSV isolates with 11 human-origin SFTS virus revealed a highly pairwise similarity, and the growth curve analysis showed nearly identical in virus yield and the dynamic of virus reproduction compared to human derived viral isolates. Immunofluorescence and neutralization test showed identical serological reaction character of the two different origin viral strains. In this study, the characters of a SFTSV isolate was firstly described, which suggested that the tick species H. longicornis acting important vector role in the transmission of SFTS virus.
authors: Shandong University, Jinan 250012, China. jxl198607@126.com
authors: Xiao-Lin
authors: XL
authors: Jiang
authors: Xian-Jun
authors: XJ
authors: Wang
authors: Jian-Dong
authors: JD
authors: Li
authors: Shu-Jun
authors: SJ
authors: Ding
authors: Quan-Fu
authors: QF
authors: Zhang
authors: Jing
authors: J
authors: Qu
authors: Shuo
authors: S
authors: Zhang
authors: Chuan
authors: C
authors: Li
authors: Wei
authors: W
authors: Wu
authors: Mei
authors: M
authors: Jiang
authors: Mi-Fang
authors: MF
authors: Liang
authors: Zhen-Qiang
authors: ZQ
authors: Bi
authors: De-Xin
authors: DX
authors: Li
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology*; D001096:Arachnid Vectors / Q000821:virology*; D002044:Bunyaviridae Infections / Q000635:transmission / Q000821:virology*; D002417:Cattle; D002460:Cell Line; D004285:Dogs; D006801:Humans; D058751:Livestock / Q000469:parasitology*; D008969:Molecular Sequence Data; D016856:Phlebovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012756:Sheep; D013987:Ticks / Q000821:virology*