title: [Simultaneous determination of three homoisoflavonoids in Ophiopogon japonicus by HPLC].
abstract: Three homoisoflavonoids, 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A from Ophiopogon japonicus were analyzed simultaneously by HPLC with acetonitrile-water containing 0.5% H3 PO4 (58:42) as the mobile phase, and the detection wavelength was set at 296 nm (0-14 min) and 275 nm (14-22 min). The mean recoveries of three homoisoflavonoids were 99.41%-99.86% (RSD 0.82%-1.05%). The linear response ranges of. 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A were 0.165-0.990 microg (r = 0.999 9), 0.153-0.918 microg (r = 0.999 9), and 0.270-1.620 microg (r = 0.999 9), respectively. This method was certified to be accurate and reliable and can be used for quality control of O. japonicus.
authors: School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400050, China.
authors: Pintao
authors: P
authors: Zeng
authors: Hui
authors: H
authors: Zhou
authors: Yimin
authors: Y
authors: Zheng
authors: Xiuying
authors: X
authors: Xu
authors: Shanquan
authors: S
authors: Fu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D007529:Isoflavones / Q000032:analysis*; D031441:Ophiopogon / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones