title: Genome wide AFLP markers support cryptic species in Coniophora (Boletales).
abstract: Numerous fungal morphospecies include cryptic species that routinely are detected by sequencing a few unlinked DNA loci. However, whether the patterns observed by multi-locus sequencing are compatible with genome wide data, such as amplified fragment length polymorphisms (AFLPs), is not well known for fungi. In this study we compared the ability of three DNA loci and AFLP data to discern between cryptic fungal lineages in the three morphospecies Coniophora olivacea, Coniophora arida, and Coniophora puteana. The sequences and the AFLP data were highly congruent in delimiting the morphotaxa into multiple cryptic species. However, while the DNA sequences indicated introgression or hybridization between some of the cryptic lineages the AFLP data did not. We conclude that as few as three polymorphic DNA loci was sufficient to recognize cryptic lineages within the studied Coniophora taxa. However, based on analyses of a few (three) sequenced loci the hybridization could not easily be distinguished from incomplete lineage sorting. Hence, great caution should be taken when concluding about hybridization based on data from just a few loci.
authors: Microbial Evolution Research Group, Department of Biology, University of Oslo, P.O. Box 1066 Blindern, N-0316 Oslo, Norway. inger.skrede@bio.uio.no
authors: Inger
authors: I
authors: Skrede
authors: Tor
authors: T
authors: Carlsen
authors: Øyvind
authors: Ø
authors: Stensrud
authors: Håvard
authors: H
authors: Kauserud
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001487:Basidiomycota / Q000145:classification* / Q000235:genetics*; D016681:Genome, Fungal; D058885:Multilocus Sequence Typing; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic