title: Identical MALDI TOF MS-derived peak profiles in a pair of isogenic SCCmec-harboring and SCCmec-lacking strains of Staphylococcus aureus.
abstract: MALDI-TOF MS-based peak differences in oxacillin-resistant Staphylococcus aureus and oxacillin-susceptible S. aureus isolates have been described previously. Unfortunately, these isolates were not isogenic with respect to their mecA gene. Ours is the first to use a SCCmec-harboring parent and a SCCmec-lacking daughter strain, with the same genetic background, to unequivocally rule out strain-specific protein peaks. We could not show differences in the peak profiles within the preset Biotyper settings used for MALDI-TOF-based identification in this pair of SCCmec-harboring parent and SCCmec-lacking daughter strains.
authors: Institute for Hygiene and Microbiology, Dept. of Medical Microbiology, Ruhr-University Bochum, Universitätsstraße 150, 44802 Bochum, Germany. Florian.szabados@rub.de
authors: Florian
authors: F
authors: Szabados
authors: Martin
authors: M
authors: Kaase
authors: Agnes
authors: A
authors: Anders
authors: Sören G
authors: SG
authors: Gatermann
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002876:Chromosomes, Bacterial; D005838:Genotype; D016106:Methicillin Resistance / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000737:chemistry* / Q000235:genetics; D046915:Penicillin-Binding Proteins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus