title: Impact of hemolysis during sample collection: how different is drug concentration in hemolyzed plasma from that of normal plasma?
abstract: Hemolysis is a common phenomenon in clinical studies. Despite the growing interest in hemolysis matrix effect, how hemolysis impacts the representability of hemolyzed plasma samples was rarely evaluated. The purpose of this research is to perform such an evaluation by theoretical consideration and experiment. A formula for estimating the impact is proposed, which includes the degree of hemolysis and the drug's red blood cell (RBC): plasma concentration ratio. The impact of hemolysis on the representability of hemolyzed plasma samples is compound-dependant. Given the same degree of hemolysis, the stronger a drug binds to RBCs, the more significant the impact of hemolysis. For a drug with high affinity to RBCs, the results of hemolyzed plasma samples may not be useful even though they are accurate. There is an overall agreement between theoretical predication and experimental results. Among the ten different drug compounds tested, only methazolamide, which binds strongly to RBCs, showed significant change in plasma concentration due to hemolysis.
authors: PharmaNet Canada Inc., Québec, Canada. aimintan@hotmail.com
authors: Aimin
authors: A
authors: Tan
authors: Sébastien
authors: S
authors: Gagné
authors: Isabelle A
authors: IA
authors: Lévesque
authors: Sylvain
authors: S
authors: Lachance
authors: Nadine
authors: N
authors: Boudreau
authors: Ann
authors: A
authors: Lévesque
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D001800:Blood Specimen Collection; D004912:Erythrocytes / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D005260:Female; D006461:Hemolysis; D006801:Humans; D008297:Male; D008704:Methazolamide / Q000097:blood; D004364:Pharmaceutical Preparations / Q000097:blood* / Q000378:metabolism; D015203:Reproducibility of Results; D012107:Research Design
chemical_list: D004364:Pharmaceutical Preparations; D008704:Methazolamide