title: BasyLiCA: a tool for automatic processing of a Bacterial Live Cell Array.
abstract: Live Cell Array (LCA) technology allows the acquisition of high-resolution time-course profiles of bacterial gene expression by the systematic assessment of fluorescence in living cells carrying either transcriptional or translational fluorescent protein fusion. However, the direct estimation of promoter activities by time-dependent derivation of the fluorescence datasets generates high levels of noise. Here, we present BasyLiCA, a user-friendly open-source interface and database dedicated to the automatic storage and standardized treatment of LCA data. Data quality reports are generated automatically. Growth rates and promoter activities are calculated by tunable discrete Kalman filters that can be set to incorporate data from biological replicates, significantly reducing the impact of noise measurement in activity estimations.
  
  
  BACKGROUND
  The BasyLiCA software and the related documentation are available at http://genome.jouy.inra.fr/basylica.
authors: INRA, UR1077 Unité Mathématique Informatique et Génome, F-78350 Jouy en Josas, France.
authors: Leslie
authors: L
authors: Aïchaoui
authors: Matthieu
authors: M
authors: Jules
authors: Ludovic
authors: L
authors: Le Chat
authors: Stéphane
authors: S
authors: Aymerich
authors: Vincent
authors: V
authors: Fromion
authors: Anne
authors: A
authors: Goelzer
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D005456:Fluorescent Dyes; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D011401:Promoter Regions, Genetic; D012984:Software; D014158:Transcription, Genetic
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins