title: LSm14A is a processing body-associated sensor of viral nucleic acids that initiates cellular antiviral response in the early phase of viral infection.
abstract: Recognition of viral nucleic acids by pattern recognition receptors initiates type I IFN induction and innate antiviral immune response. Here we show that LSm14A, a member of the LSm family involved in RNA processing in the processing bodies, binds to synthetic or viral RNA and DNA and mediates IRF3 activation and IFN-β induction. Knockdown of LSm14A inhibits cytosolic RNA- and DNA-trigger type I IFN production and cellular antiviral response. Moreover, LSm14A is essential for early-phase induction of IFN-β after either RNA or DNA virus infection. We further found that LSm14A-mediated IFN-β induction requires RIG-I-VISA or MITA after RNA or DNA virus infection, respectively, and viral infection causes translocation of LSm14A to peroxisomes, where RIG-I, VISA, and MITA are located. These findings suggest that LSm14A is a sensor for both viral RNA and DNA and plays an important role in initiating IFN-β induction in the early phase of viral infection.
authors: State Key Laboratory of Virology, College of Life Sciences,  Wuhan University, Wuhan 430072,  China.
authors: Ying
authors: Y
authors: Li
authors: Rui
authors: R
authors: Chen
authors: Qian
authors: Q
authors: Zhou
authors: Zhisheng
authors: Z
authors: Xu
authors: Chao
authors: C
authors: Li
authors: Shuai
authors: S
authors: Wang
authors: Aiping
authors: A
authors: Mao
authors: Xiaodong
authors: X
authors: Zhang
authors: Weiwu
authors: W
authors: He
authors: Hong-Bing
authors: HB
authors: Shu
mesh_terms: D045744:Cell Line, Tumor; D004266:DNA Virus Infections / Q000276:immunology*; D004279:DNA, Viral / Q000276:immunology*; D055785:Gene Knockdown Techniques; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism; D016899:Interferon-beta / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics; D009841:Oligonucleotides / Q000235:genetics; D034622:RNA Interference; D012327:RNA Virus Infections / Q000276:immunology*; D012367:RNA, Viral / Q000276:immunology*; D012261:Ribonucleoproteins / Q000235:genetics / Q000276:immunology*; D000099197:STING Protein
chemical_list: D004279:DNA, Viral; D050838:Interferon Regulatory Factor-3; D016899:Interferon-beta; D008565:Membrane Proteins; D009841:Oligonucleotides; D012367:RNA, Viral; D012261:Ribonucleoproteins; C494232:IRF3 protein, human; C510649:RNA-associated protein 55, human; C532857:STING1 protein, human; D000099197:STING Protein