title: Total flavonoids from Rosa Laevigata Michx fruit attenuates hydrogen peroxide induced injury in human umbilical vein endothelial cells.
abstract: The aim of the present study was to evaluate the protective effect and possible mechanism of the total flavonoids (TFs) from Rosa Laevigata Michx fruit (RLMF) against hydrogen peroxide (H(2)O(2)) induced damage in human umbilical vein endothelial cells (HUVECs). The cell injury caused by H(2)O(2) was protected by pretreatment with the TFs for 1h. Compared with the model group, the TFs decreased S phase cells, suppressed nuclear morphological damage, inhibited the collapse of mitochondrial membrane potentials (ΔΨm), attenuated excessive reactive oxygen species generation, reduced glutathione depletion, impacted the mitochondrial morphology change, decreased caspase-3, -9 activities, and decreased fragmented DNA. Further mechanism investigation showed that the TFs could increase the protein expressions of Procaspase-3, Bcl-2, and decrease the expressions of Bak, Bax, Bid and p53. Generally, the TFs from RLMF is an effective natural product for the treatment of cardiovascular and cerebrovascular diseases.
authors: College of Pharmacy, Dalian Medical University, No. 9 Western Lvshun South Road, Dalian 116044, China.
authors: Yannan
authors: Y
authors: Jia
authors: Liu
authors: L
authors: Ji
authors: Shuai
authors: S
authors: Zhang
authors: Lina
authors: L
authors: Xu
authors: Lianhong
authors: L
authors: Yin
authors: Lei
authors: L
authors: Li
authors: Yanyan
authors: Y
authors: Zhao
authors: Jinyong
authors: J
authors: Peng
mesh_terms: D015153:Blotting, Western; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D005434:Flow Cytometry; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity; D020287:In Situ Nick-End Labeling; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D009569:Nitric Oxide / Q000096:biosynthesis; D017382:Reactive Oxygen Species / Q000378:metabolism; D027826:Rosa / Q000737:chemistry*; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D005419:Flavonoids; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D005978:Glutathione