title: Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range.
abstract: Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins.
authors: EMBL, Structural and Computational Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors: Sigrid
authors: S
authors: Milles
authors: Christine
authors: C
authors: Koehler
authors: Yann
authors: Y
authors: Gambin
authors: Ashok A
authors: AA
authors: Deniz
authors: Edward A
authors: EA
authors: Lemke
mesh_terms: D002232:Carbocyanines; D003116:Color; D004926:Escherichia coli / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D006801:Humans; D006834:Hydrazines; D057054:Molecular Imaging / Q000379:methods*; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry* / Q000235:genetics; D009930:Organic Chemicals; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods*
chemical_list: C417561:Alexa 488 hydrazide; C569686:Alexa Fluor 647; C417664:Alexa594; D002232:Carbocyanines; D005456:Fluorescent Dyes; D006834:Hydrazines; C077727:NUP49 protein, S cerevisiae; D028861:Nuclear Pore Complex Proteins; D009930:Organic Chemicals; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins