title: In vitro comparison of the biological activity of alumina ceramic and titanium particles associated with aseptic loosening.
abstract: Prosthetic wear particles are thought to play a central role in the initiation and development of periprosthetic osteolysis, leading to aseptic loosening of prostheses. This study aimed to compare the biological activity of ceramic and titanium particles that are associated with particle-induced, aseptic joint loosening. Different sizes of alumina-ceramic particles and titanium particles were prepared to stimulate murine macrophage cells RAW 264.7, of which the expressions of tumor necrosis factor alpha (TNF-alpha) and receptor activator of nuclear factor-κB ligand (RANKL) were measured by qPCR and ELISA at various time points. In the presence of all particles, the expression of TNF-alpha increased in a time-dependent manner, whereas the expression of RANKL showed no regular expression patterns. Notably, particles of smaller sizes provoked significantly higher levels of TNF-alpha and RANKL than those of larger sizes. Compared to the titanium particles, the ceramic particles provoked a significantly lower production of TNF-alpha. Thus, the bioactivities of titanium and alumina ceramic particles were inversely proportional to the sizes of the particles, and the expression of RANKL was not parallel to that of TNF-alpha. The successful outcome of ceramic-on-ceramic artificial joint prostheses may be attributed to the low biological activity of ceramic particles, as evidenced here.
authors: Department of Orthopaedic Surgery, The Sun Yat-Sen Memorial's Hospital of Sun Yat-Sen University, 107 Yanjiangxi Road, 510120, Guangzhou, People's Republic of China. dingyue36@126.com
authors: Yue
authors: Y
authors: Ding
authors: Chu-qiang
authors: CQ
authors: Qin
authors: Yu-ru
authors: YR
authors: Fu
authors: Jie
authors: J
authors: Xu
authors: Dong-sheng
authors: DS
authors: Huang
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D016207:Cytokines / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D007249:Inflammation; D007595:Joint Prosthesis; D008264:Macrophages / Q000166:cytology; D051379:Mice; D010014:Osteolysis; D010316:Particle Size; D016133:Polymerase Chain Reaction / Q000379:methods; D011474:Prosthesis Design; D053245:RANK Ligand / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D053245:RANK Ligand; D014409:Tumor Necrosis Factor-alpha; D014025:Titanium; D000537:Aluminum Oxide