title: Increased E-selectin in hepatic ischemia-reperfusion injury mediates             liver metastasis of pancreatic cancer.
abstract: Several recent studies have reported that selectins are produced during             ischemia-reperfusion injury, and that selectin ligands play an important role             in cell binding to the endothelium and in liver metastasis. Portal clamping during             pancreaticoduodenectomy with vessel resection for pancreatic head cancer causes             hepatic ischemia-reperfusion injury, which might promote liver metastasis. We             investigated the liver colonization of pancreatic cancer cells under hepatic ischemia-reperfusion             and examined the involvement of E-selectin and its ligands. A human pancreatic             cancer cell line (Capan-1) was injected into the spleen of mice after hepatic             ischemia-reperfusion (I/R group). In addition, to investigate the effect of an             anti-E-selectin antibody on liver colonization in the IR group, mice received             an intraperitoneal injection of the anti-E-selectin antibody following hepatic             ischemia-reperfusion and tumor inoculation (IR+Ab group). Four weeks later, mice             were sacrificed and the number of tumor nodules on the liver was compared to mice             without hepatic ischemia-reperfusion (control group). The incidence of liver metastasis             in the I/R group was significantly higher (16 of 20, 80%) than that in the control             group (6 of 20, 30%) (P<0.01). Moreover, mice in the I/R group had significantly             more tumor nodules compared to those in the control group (median, 9.9 vs. 2.7             nodules) (P<0.01). In the I/R+Ab group, only 2 of 5 (40%) mice developed liver             metastases. RT-PCR and southern blotting of the liver extracts showed that the             expression of IL-1 and E-selectin mRNA after hepatic ischemia-reperfusion was             significantly higher than the basal levels. Hepatic ischemia-reperfusion increases             liver metastases and E-selectin expression in pancreatic cancer. These results             suggest that E-selectin produced due to hepatic ischemia-reperfusion is involved             in liver metastasis.
authors: Department of Gastroenterologic Surgery, Division of Cancer Medicine,                     Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.
authors: Katsuhiro
authors: K
authors: Yoshimoto
authors: Hidehiro
authors: H
authors: Tajima
authors: Tetsuo
authors: T
authors: Ohta
authors: Koichi
authors: K
authors: Okamoto
authors: Seisho
authors: S
authors: Sakai
authors: Jun
authors: J
authors: Kinoshita
authors: Hiroyuki
authors: H
authors: Furukawa
authors: Isamu
authors: I
authors: Makino
authors: Hironori
authors: H
authors: Hayashi
authors: Keishi
authors: K
authors: Nakamura
authors: Katsunobu
authors: K
authors: Oyama
authors: Masafumi
authors: M
authors: Inokuchi
authors: Hisatoshi
authors: H
authors: Nakagawara
authors: Hiroshi
authors: H
authors: Itoh
authors: Hideto
authors: H
authors: Fujita
authors: Hiroyuki
authors: H
authors: Takamura
authors: Itasu
authors: I
authors: Ninomiya
authors: Hirohisa
authors: H
authors: Kitagawa
authors: Sachio
authors: S
authors: Fushida
authors: Takashi
authors: T
authors: Fujimura
authors: Tomohiko
authors: T
authors: Wakayama
authors: Shoichi
authors: S
authors: Iseki
authors: Koichi
authors: K
authors: Shimizu
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology / Q000378:metabolism / Q000556:secondary*; D000328:Adult; D000818:Animals; D045744:Cell Line, Tumor; D019040:E-Selectin / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism; D008113:Liver Neoplasms / Q000209:etiology / Q000378:metabolism / Q000556:secondary*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D015427:Reperfusion Injury / Q000150:complications / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D019040:E-Selectin; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha