title: Broad resistance to ACCase inhibiting herbicides in a ryegrass population is due only to a cysteine to arginine mutation in the target enzyme.
abstract: BACKGROUND
  The design of sustainable weed management strategies requires a good understanding of the mechanisms by which weeds evolve resistance to herbicides. Here we have conducted a study on the mechanism of resistance to ACCase inhibiting herbicides in a Lolium multiflorum population (RG3) from the UK.
  
  
  RESULTS
  Analysis of plant phenotypes and genotypes showed that all the RG3 plants (72%) that contained the cysteine to arginine mutation at ACCase codon position 2088 were resistant to ACCase inhibiting herbicides. Whole plant dose response tests on predetermined wild and mutant 2088 genotypes from RG3 and a standard sensitive population indicated that the C2088R mutation is the only factor conferring resistance to all ten ACCase herbicides tested. The associated resistance indices ranged from 13 for clethodim to over 358 for diclofop-methyl. Clethodim, the most potent herbicide was significantly affected even when applied on small mutant plants at the peri-emergence and one leaf stages.
  
  
  CONCLUSIONS
  This study establishes the clear and unambiguous importance of the C2088R target site mutation in conferring broad resistance to ten commonly used ACCase inhibiting herbicides. It also demonstrates that low levels "creeping", multigenic, non target site resistance, is not always selected before single gene target site resistance appears in grass weed populations subjected to herbicide selection pressure.
authors: Syngenta, Jealott's Hill International Research Centre, Biological Sciences, Bracknell, United Kingdom. deepak.kaundun@syngenta.com
authors: Shiv Shankhar
authors: SS
authors: Kaundun
authors: Sarah-Jane
authors: SJ
authors: Hutchings
authors: Richard Paul
authors: RP
authors: Dale
authors: Eddie
authors: E
authors: McIndoe
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors* / Q000235:genetics; D001120:Arginine / Q000235:genetics*; D003512:Cyclohexanones; D003545:Cysteine / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D005828:Genetics, Population; D060005:Genotyping Techniques; D053209:Herbicide Resistance / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology*; D006574:Heterocyclic Compounds, 2-Ring / Q000494:pharmacology; D008129:Lolium / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D011422:Propionates / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D015203:Reproducibility of Results; D012639:Seeds / Q000187:drug effects; D012641:Selection, Genetic
chemical_list: D003512:Cyclohexanones; D006540:Herbicides; D006574:Heterocyclic Compounds, 2-Ring; D011422:Propionates; D011725:Pyridines; C441919:clethodim; D001120:Arginine; D000103:Acetyl-CoA Carboxylase; D003545:Cysteine; C561036:clodinafop-propargyl; C542519:pinoxaden