title: A look at diacylglycerol acyltransferases (DGATs) in algae.
abstract: Triacylglycerols (TAGs) from algae are considered to be a potentially viable source of biodiesel and thereby renewable energy, but at the moment very little is known about the biosynthetic pathway in these organisms. Here we compare what is currently known in eukaryotic algal species, in particular the characteristics of algal diacylglycerol acyltransferase (DGAT), the last enzyme of de novo TAG biosynthesis. Several studies in plants and mammals have shown that there are two DGAT isoforms, DGAT1 and DGAT2, which catalyse the same reaction but have no clear sequence similarities. Instead, they have differences in functionality and spatial and temporal expression patterns. Bioinformatic searches of sequenced algal genomes reveal that most algae have multiple copies of putative DGAT2s, whereas other eukaryotes have single genes. Investigating whether these putative isoforms are indeed functional and whether they confer significantly different phenotypes to algal cells will be vital for future efforts to genetically modify algae for biofuel production.
authors: Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
authors: Jit Ern
authors: JE
authors: Chen
authors: Alison G
authors: AG
authors: Smith
mesh_terms: D020418:Algal Proteins / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics; D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D000460:Chlorophyta / Q000201:enzymology* / Q000235:genetics; D019295:Computational Biology; D030562:Databases, Protein; D051048:Diacylglycerol O-Acyltransferase / Q000737:chemistry* / Q000235:genetics; D017377:Diatoms / Q000201:enzymology* / Q000235:genetics; D053858:Metabolic Networks and Pathways; D051379:Mice; D008969:Molecular Sequence Data; D000459:Phaeophyceae / Q000201:enzymology / Q000235:genetics; D010802:Phylogeny; D000461:Rhodophyta / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D020418:Algal Proteins; D029681:Arabidopsis Proteins; D056804:Biofuels; D029701:Saccharomyces cerevisiae Proteins; D051048:Diacylglycerol O-Acyltransferase