title: Lactobacillus casei ssp.casei induced Th1 cytokine profile and natural killer cells activity in invasive ductal carcinoma bearing mice.
abstract: Lactic acid bacteria which are used as probiotics have ability to modulate immune responses and modify immune mechanisms. It has also been indicated that some strains of this family can affect the immune responses against solid tumors. In the present work, we proposed to study the effects of oral administration of L.cacesi ssp casei on the NK cells cytotoxicity and also production of cytokines in spleen cells culture of BALB/c mice bearing invasive ductal carcinoma. 30 female In-bred BALB/c mice, were used and divided in two groups of test and control each containing 15 mice. Every day from 2 weeks before tumor transplantation 0.5 ml of PBS containing 2.7×108 CFU/ml of L.casei spp casei was orally administered to the test mice and it was followed 3 weeks after transplantation as well with 3 days interval between each week. Control mice received an equal volume of PBS in a same manner. Results showed that oral administration of L. casei significantly increased the production of IL-12 and IFN-γ (P<0.05) and increased the natural killer cells (NK) cytotoxicity in spleen cells culture of test mice (P<0.05). It has also been demonstrated that the growth rate of tumor in the test mice was decreased and their survival was significantly prolonged in comparison to the controls. Our findings suggest that daily intake of L.casei can improve immune responses in mice bearing invasive ductal carcinoma, but further studies are needed to investigate the other involving mechanisms in this case.
authors: Department of Pharmaceutical Biotechnology, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohammad Mehdi
authors: MM
authors: Soltan Dallal
authors: Mohammad Hossein
authors: MH
authors: Yazdi
authors: Marzieh
authors: M
authors: Holakuyee
authors: Zuhair Mohammad
authors: ZM
authors: Hassan
authors: Mohsen
authors: M
authors: Abolhassani
authors: Mehdi
authors: M
authors: Mahdavi
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D018270:Carcinoma, Ductal, Breast / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology*; D007780:Lacticaseibacillus casei / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D019936:Probiotics; D018417:Th1 Cells / Q000276:immunology*; D013997:Time Factors; D047368:Tumor Burden
chemical_list: D016207:Cytokines; D018664:Interleukin-12; D007371:Interferon-gamma