title: Color-encoded microcarriers based on nano-silicon dioxide film for multiplexed immunoassays.
abstract: Multiplexed analysis allows researchers to obtain high-density information with minimal assay time, sample volume and cost. Currently, microcarrier or particle-based approaches for multiplexed analysis involve complicated or expensive encoding and decoding processes. In this paper, a novel optical encoding technique based on nano-silicon dioxide film is presented. Microcarriers composed of thermally grown silicon dioxide (SiO(2)) film and monocrystalline silicon (Si) substrate were fabricated. The nano-silicon dioxide film exhibited unique surface color by low-coherence interference. Hence the colors can be used for encoding at least 100 microcarriers loaded with films of different thickness. We demonstrated that color-encoded microcarriers loaded with antigens could be used for multiplexed immunoassays to detect goat anti-human IgG, goat anti-mouse IgG and goat anti-rabbit IgG, with fluorescent detection as the interrogating approach. This microcarrier-based method also exhibited improved analytical performance compared with a microarray technique. This approach will provide new opportunities for multiplexed target assay development.
authors: Department of Biomedical Engineering, Tsinghua University School of Medicine, Beijing 100084, China.
authors: Qiang
authors: Q
authors: Li
authors: Kaihuan
authors: K
authors: Zhang
authors: Tongzhou
authors: T
authors: Wang
authors: Xinying
authors: X
authors: Zhou
authors: Jia
authors: J
authors: Wang
authors: Chen
authors: C
authors: Wang
authors: Haixiao
authors: H
authors: Lin
authors: Xin
authors: X
authors: Li
authors: Ying
authors: Y
authors: Lu
authors: Guoliang
authors: G
authors: Huang
mesh_terms: D000818:Animals; D003116:Color; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000379:methods*; D055616:Microtechnology / Q000379:methods*; D008962:Models, Theoretical; D049329:Nanostructures / Q000737:chemistry*; D055620:Optical Phenomena; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide