title: Cytotoxic ellagitannins from Reaumuria vermiculata.
abstract: Three ellagitannins and one disulfated flavonol were isolated from the aerial parts of Reaumuria vermiculata L. Besides that, 16 known compounds were characterized as well. The structures of all compounds were elucidated on the basis of spectroscopic data including 1D and 2D NMR and ESI HR-FTMS. The in vivo antioxidant activity using the oxygen radical absorbance capacity (ORAC) method, of the extract, its column fractions and two of the isolated ellagitannins was accomplished. In addition, a possible cytotoxicity of the extract and two of the new ellagitannins on HaCaT human keratinocytes and the activity of both compounds against the prostate cancer cell line (PC-3) were also assessed, whereby a potent cytotoxicity with IC₅₀ less than 1 μg/ml was determined for both compounds. Besides, the extract exhibited a potential cytotoxic effect against four different solid tumor cell lines, namely liver (Huh-7), colorectal (HCT-116), breast (MCF-7) and prostate (PC-3). The IC₅₀s were found to be substantially low (ranged from 1.3±0.15 to 2.4±0.22 μg/ml) with relatively low resistance possibility reaching to 0% in the case of Huh-7 cell.
authors: Department of Phytochemistry and Plant Systematics, National Research Center, Dokki, Cairo, Egypt. mahmoudnawwarhesham@yahoo.com
authors: Mahmoud A
authors: MA
authors: Nawwar
authors: Nahla A
authors: NA
authors: Ayoub
authors: Mohamed A
authors: MA
authors: El-Rai
authors: Fatma
authors: F
authors: Bassyouny
authors: Eman S
authors: ES
authors: Mostafa
authors: Ahmed M
authors: AM
authors: Al-Abd
authors: Manuela
authors: M
authors: Harms
authors: Kristian
authors: K
authors: Wende
authors: Ulrike
authors: U
authors: Lindequist
authors: Michael W
authors: MW
authors: Linscheid
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D002277:Carcinoma / Q000188:drug therapy; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D047348:Hydrolyzable Tannins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D015603:Keratinocytes / Q000187:drug effects; D008297:Male; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy*; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D032363:Tamaricaceae / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D047348:Hydrolyzable Tannins; D010936:Plant Extracts