title: Multi-color miniature dual-axis confocal microscope for point-of-care pathology.
abstract: We present a miniature microelectromechanical systems-based dual-axis confocal microscope capable of spatially coregistered fluorescence and reflectance imaging at multiple wavelengths. This device has a 10 mm diameter scan head with a 2 mm diameter tip for convenient use during surgery to guide tumor resection. The microscope has an adjustable focal depth of 20-200 micrometers and is capable of imaging with an axial resolution of 9 micrometers and in-plane resolution of 4 micrometers over a field of view of 450×450 micrometers. Simultaneous two-color imaging of individual optical sections is achieved by using a pair of grating-prism assemblies to compensate for chromatic dispersion in the 2 mm diameter gradient index relay lens at the distal tip of the device. Experimental measurements of the axial response of the microscope, as well as two-color images of a reflective bar target and fresh mouse brain tissues, demonstrate the performance of our device and its potential for multicolor in vivo optical sectioning microscopy.
authors: Stony Brook University (SUNY), Department of Biomedical Engineering, Stony Brook, NY 11794, USA.
authors: Steven Y
authors: SY
authors: Leigh
authors: Jonathan T C
authors: JT
authors: Liu
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D003116:Color; D021621:Imaging, Three-Dimensional; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation*; D055616:Microtechnology / Q000295:instrumentation*; D010336:Pathology / Q000295:instrumentation*; D019095:Point-of-Care Systems