title: Ligation of Fc gamma receptor IIB enhances levels of antiviral cytokine in response to PRRSV infection in vitro.
abstract: PRRSV infection ADE facilitates the attachment and internalization of the virus onto its host cells, such as monocytes and macrophages, through Fc receptor-mediated endocytosis. FcγRIIB is the only inhibitory receptor with a tyrosine-based inhibitory motif (ITIM) in its cytoplasmic tail, where counters the "ITAM triggered" activation signals and down-regulates phagocytosis. However, porcine FcγRIIB's role in the antiviral immune response to PRRSV infection has not been studied. In this study, our results indicated that selective activation of porcine FcγRIIB in PAM cells up-regulated significantly mRNA levels of IFN-α and TNF-α at any time point post-pretreatment, suggesting that porcine FcγRIIB signal can enhance the innate antiviral response of host cells. PRRSV infection assay mediated by FcγRIIB indicated that selective activation of porcine FcγRIIB in PAM cells enhanced mRNA levels of antiviral cytokine (IFN-α and TNF-α) and repressed mRNA levels of IL-10 in response to PRRSV infection, suggesting that FcγRIIB ligation can enhance the antiviral immune response to PRRSV infection. In addition, FcγRIIB ligation to infection indicated that PRRSV replication in PAM was not positive correlation with increasing of IFN-α mRNA levels and decreasing of IL-10 mRNA levels, suggesting that there is complex viral replication mechanism in immune cells such as PAM for PRRSV.
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors: Yina
authors: Y
authors: Zhang
authors: Yonghui
authors: Y
authors: Zhou
authors: Qingyuan
authors: Q
authors: Yang
authors: Chunlong
authors: C
authors: Mu
authors: Erzhen
authors: E
authors: Duan
authors: Jing
authors: J
authors: Chen
authors: Mingfan
authors: M
authors: Yang
authors: Pingan
authors: P
authors: Xia
authors: Baoan
authors: B
authors: Cui
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics* / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000503:physiopathology*; D019316:Porcine respiratory and reproductive syndrome virus; D012333:RNA, Messenger / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism*; D013552:Swine; D013997:Time Factors
chemical_list: D016207:Cytokines; C080571:Fc gamma receptor IIB; D012333:RNA, Messenger; D017452:Receptors, IgG