title: A novel behavioral assay for measuring cold sensation in mice.
abstract: Behavioral models of cold responses are important tools for exploring the molecular mechanisms of cold sensation. To complement the currently cold behavioral assays and allow further studies of these mechanisms, we have developed a new technique to measure the cold response threshold, the cold plantar assay. In this assay, animals are acclimated on a glass plate and a cold stimulus is applied to the hindpaw through the glass using a pellet of compressed dry ice. The latency to withdrawal from the cooled glass is used as a measure of the cold response threshold of the rodents, and the dry ice pellet provides a ramping cold stimulus on the glass that allows the correlation of withdrawal latency values to rough estimates of the cold response threshold temperature. The assay is highly sensitive to manipulations including morphine-induced analgesia, Complete Freund's Adjuvant-induced inflammatory allodynia, and Spinal Nerve Ligation-induced neuropathic allodynia.
authors: Washington University Pain Center and Department of Anesthesiology, Washington University School of Medicine, St. Louis, Missouri, United States of America.
authors: Daniel S
authors: DS
authors: Brenner
authors: Judith P
authors: JP
authors: Golden
authors: Robert W
authors: RW
authors: Gereau
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D003080:Cold Temperature; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010147:Pain Measurement / Q000379:methods*; D058748:Pain Perception / Q000502:physiology*; D017288:Pain Threshold / Q000502:physiology; D013697:Thermosensing / Q000502:physiology*