title: Synthesis of heterobimetallic complexes: in vitro DNA binding, cleavage and antimicrobial studies.
abstract: The monometallic (1-3) and heterobimetallic (4-6) complexes, containing 1,10-phenanthroline and indole-3-acetic acid were synthesized and characterized by spectroscopic (IR, UV-vis, NMR, ESI-MS) and analytical methods. The in vitro DNA binding studies of 5 and 6 with CT DNA were carried out by employing various biophysical methods which reveal strong electrostatic binding via phosphate backbone of DNA helix. The binding constant (K) value as determined from fluorescence experiments of complexes 5 and 6 were calculated to be 4.09×10(4) and 2.51×10(4) M(-1), respectively revealing that complex 5 has greater binding propensity for DNA. To gain further insight into the molecular recognition at the target site, interaction studies of 5 with 5'-GMP were carried out by employing (1)H and (31)P NMR spectroscopy. Complex 5 exhibited preferential selectively towards the minor groove of pBR322 DNA and efficient cleavage activity via hydrolytic pathway. Furthermore complexes 4-6 exhibited significant antimicrobial activity.
authors: Department of Chemistry, Aligarh Muslim University, Aligarh 202 002, India. tsartaj62@yahoo.com
authors: Sartaj
authors: S
authors: Tabassum
authors: Mehvash
authors: M
authors: Zaki
authors: Farukh
authors: F
authors: Arjmand
authors: Iqbal
authors: I
authors: Ahmad
mesh_terms: D000890:Anti-Infective Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D003035:Cobalt / Q000737:chemistry; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D007210:Indoleacetic Acids / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry; D055672:Static Electricity
chemical_list: D000890:Anti-Infective Agents; D056831:Coordination Complexes; D007210:Indoleacetic Acids; D010618:Phenanthrolines; D003035:Cobalt; C030737:indoleacetic acid; D003300:Copper; D004247:DNA; C025205:1,10-phenanthroline