title: Impact of emerging pollutants on pulmonary inflammation in asthmatic rats: ethanol vapors and agglomerated TiO2 nanoparticles.
abstract: BACKGROUND
  Titanium dioxide nanoparticles (nano-TiO(2)) and ethanol vapors are air contaminants with increasing importance. The presence of a pathological pulmonary condition, such as asthma, may increase lung susceptibility to such contaminants.
  
  
  OBJECTIVE
  This study aimed to investigate if exposure to inhaled ethanol vapors or nano-TiO(2) can modulate the rat pulmonary inflammatory response resulting from an allergic asthmatic reaction.
  
  
  METHODS
  Brown Norway rats were sensitized (sc) and challenged (15 min inhalation, 14 days later) with chicken egg ovalbumin (OVA). Leukocytes were counted in bronchoalveolar lavages (BAL) performed at 6, 24, 36, 48 and 72 h following the challenge and either after ethanol exposures (3000 ppm, 6 h/day, daily) or at 48 h (peak inflammation) for nano-TiO(2) exposures (9.35 mg/m(3) aerosol for 6 and 42 h after the OVA challenge). For the nano-TiO(2) exposures, plasma and BAL cytokines were measured and lung histological analyzes were performed.
  
  
  RESULTS
  Exposure to ethanol did not significantly affect BAL leukocytes after OVA challenge. Exposure to nano-TiO(2) significantly decreased BAL leukocytes compared to OVA-challenged controls. Plasma and BAL IL-4, IL-6, and INF-γ levels were also decreased in the nano-TiO(2) group.
  
  
  CONCLUSIONS
  While ethanol vapors do not modify the pulmonary inflammation in rats during an asthmatic response, a surprising protective effect for agglomerated nano-TiO(2) was observed. A putative mechanistic basis involving a decrease in the Th2 response caused by OVA is proposed.
  
  
  CONCLUSIONS
  Allergic pulmonary inflammation is not up-regulated by inhalation of the pollutants ethanol and nano-TiO(2). On the contrary, nano-TiO(2) decreases lung inflammation in asthmatic rats.
authors: INRS-Institut Armand-Frappier, Université du Québec, Laval, Quebec, Canada.
authors: A
authors: A
authors: Scarino
authors: A
authors: A
authors: Noël
authors: P M
authors: PM
authors: Renzi
authors: Y
authors: Y
authors: Cloutier
authors: R
authors: R
authors: Vincent
authors: G
authors: G
authors: Truchon
authors: R
authors: R
authors: Tardif
authors: M
authors: M
authors: Charbonneau
mesh_terms: D000336:Aerosols; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001249:Asthma / Q000097:blood / Q000150:complications* / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D005260:Female; D019570:Inhalation Exposure; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D053758:Nanoparticles / Q000633:toxicity*; D010047:Ovalbumin / Q000276:immunology; D011014:Pneumonia / Q000139:chemically induced* / Q000150:complications / Q000276:immunology; D051381:Rats; D011914:Rats, Inbred BN; D014025:Titanium / Q000633:toxicity*; D014835:Volatilization
chemical_list: D000336:Aerosols; D000393:Air Pollutants; D016207:Cytokines; C009495:titanium dioxide; D000431:Ethanol; D010047:Ovalbumin; D014025:Titanium