title: RANTES, MCP-1 chemokines and factors describing rheumatoid arthritis.
abstract: The MCP-1/CCL2 as well as RANTES/CCL5 chemokines are potent chemoattractants involved in immunoregulatory and inflammatory processes of rheumatoid arthritis. Recent studies demonstrated elevated levels of MCP-1 and RANTES in plasma, synovial fluid, and the synovial tissue of patients with RA. To examine the relationship among MCP-1 and RANTES single nucleotide polymorphisms and circulating levels and rheumatoid arthritis (RA), a total of 156 RA patients and 125 controls were recruited into the study. An association of -855 C/G MCP-1 polymorphism to IgM RF within the RA patients was observed. The lowest circulating levels of RANTES were observed in the AA variant of RANTES -403 G/A polymorphism. Furthermore, an association of -403 AA variant to circulating levels of IL-15 and IL-10 was found. No associations of factors describing rheumatoid arthritis (RFs, ANA, anti-CCP-positive/negative, DAS 28 score and number of swollen joints) with MCP-1 levels, genotype distribution, allelic frequencies and/or frequencies of haplotypes composed of all three studied polymorphisms in promoter region of MCP-1, and RANTES polymorphism were observed. We conclude that the RANTES promoter polymorphism is associated to circulating levels of RANTES, IL15 and IL10. However, our findings suggest that polymorphisms in the MCP-1 and RANTES gene promoters do not contribute significantly to the interindividual RA susceptibility and/or severity in Caucasians.
authors: Institute of Pathological Physiology, Masaryk University, Brno, Czech Republic. goldberg@med.muni.cz
authors: Monika
authors: M
authors: Pavkova Goldbergova
authors: Jolana
authors: J
authors: Lipkova
authors: Nikolas
authors: N
authors: Pavek
authors: Jindra
authors: J
authors: Gatterova
authors: Anna
authors: A
authors: Vasku
authors: Miroslav
authors: M
authors: Soucek
authors: Petr
authors: P
authors: Nemec
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000276:immunology*; D018932:Chemokine CCL2 / Q000097:blood* / Q000235:genetics* / Q000276:immunology; D018946:Chemokine CCL5 / Q000097:blood* / Q000235:genetics* / Q000276:immunology; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000235:genetics / Q000276:immunology; D016753:Interleukin-10 / Q000097:blood*; D019409:Interleukin-15 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D013582:Synovial Fluid / Q000378:metabolism; D013583:Synovial Membrane / Q000378:metabolism; D055815:Young Adult
chemical_list: C111835:CCL2 protein, human; C483024:CCL5 protein, human; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D007075:Immunoglobulin M; D019409:Interleukin-15; D016753:Interleukin-10