title: Nonaqueous capillary electrophoresis of imatinib mesylate and related substances.
abstract: In the present study, nonaqueous capillary electrophoretic separation of imatinib mesylate (IM) and related substances, N-(5-amino-2-methylphenyl)-4-(3-pyridyl)-2-pyrimidinamine (PYA), N-(4-methyl-3-(4-(pyridin-3-yl)pyrimidin-2-ylamino)phenyl)-4-((piperazin-1-yl)methyl) benzamide (NDI) and 4-chloromethyl-N-(4-methyl-3-((4-(pyridin-3-yl) pyrimidin-2-yl) amino) phenyl) benzamide (CPB) was developed. The influential factors affecting separation, including type and concentration of the electrolyte, applied voltage, and buffer modifier were investigated. Baseline separation of the studied analytes was obtained using a buffer of 50 mM Tris and 50 mM methanesulfonic acid in methanol at a apparent pH (pH*) of 1.65. To enhance the sensitivity, large-volume sample stacking was employed for online concentration. The strongest analytical signal with a suitable separation was achieved when the injection time was 100 s. The linearity ranges of PYA and NDI were 0.100-2.50 μg mL(-1), and that of CPB was 0.125-2.50 μg mL(-1), with good coefficients (r(2) > 0.9948). The relative standard deviations of intra- and interday were satisfactory. Under the optimized conditions, seven batches of the synthesized samples were analyzed and CPB was detected in two batches. Owing to its simplicity, effectiveness, and low price, the developed method is promising for quality control of IM.
authors: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, China.
authors: Lei
authors: L
authors: Ye
authors: Yifei
authors: Y
authors: Huang
authors: Jian
authors: J
authors: Li
authors: Guangya
authors: G
authors: Xiang
authors: Li
authors: L
authors: Xu
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis* / Q000737:chemistry; D001549:Benzamides; D019075:Electrophoresis, Capillary / Q000379:methods*; D000068877:Imatinib Mesylate; D010879:Piperazines / Q000032:analysis* / Q000737:chemistry; D011743:Pyrimidines / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D001549:Benzamides; D010879:Piperazines; D011743:Pyrimidines; D000068877:Imatinib Mesylate