title: Investigation of the antitrypanosomal activity of Buchholzia coriacea seed extract against a field strain of Trypanosoma congolense.
abstract: The antitrypanosomal activity of the methanol extract of Buchholzia coriacea seed against a field strain of Trypanosoma congolense was investigated using experimentally infected mice of both sexes. Monitoring of parasitaemia was by the rapid matching technique. When parasitaemia was approximately log 7.8 (63 × 10(6) parasites/ml), treatment with graded doses of the extract (250, 500 and 1000 mg/kg) was instituted for 5 consecutive days. Diminazene diaceturate (Dimivet SKM Pharma Pvt. Ltd.) was given at 3.5 mg/kg i.p. to the positive control mice. No significant differences in body weights were observed. The rectal temperatures of infected mice showed fluctuations. The PCV of infected mice were significantly (p < 0.05) lower than those of the uninfected controls. There was no significant difference between the PCV of the extract-treated and untreated animals. Parasitaemia increased steadily in the extract-treated and untreated mice groups till all the animals died. Three days post-treatment with diminazene diaceturate parasitaemia was cleared. Six days later, there was a relapse of infection. By the end of the experiment, a 50 % relapse rate was recorded in the diminazene diaceturate-treated group. The methanol extract of Buchholzia coriacea seeds did not show any antitrypanosomal activity in mice infected with Trypanosoma congolense at the doses tested.
authors: Department of Veterinary Medicine, University of Nigeria, Nsukka. Nwakaego_ernestina@yahoo.com
authors: N E
authors: NE
authors: Nweze
authors: B M
authors: BM
authors: Anene
authors: I U
authors: IU
authors: Asuzu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D031483:Capparaceae / Q000737:chemistry*; D004133:Diminazene / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D005260:Female; D006400:Hematocrit; D008297:Male; D000432:Methanol; D051379:Mice; D018512:Parasitemia / Q000188:drug therapy / Q000662:veterinary; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D014344:Trypanocidal Agents / Q000494:pharmacology / Q000627:therapeutic use*; D014348:Trypanosoma congolense / Q000187:drug effects* / Q000302:isolation & purification; D014353:Trypanosomiasis, African / Q000188:drug therapy / Q000469:parasitology
chemical_list: D010936:Plant Extracts; D014344:Trypanocidal Agents; C003915:diminazene aceturate; D000432:Methanol; D004133:Diminazene