title: Chemical composition and vasodilatation induced by Cuphea carthagenensis preparations.
abstract: The aerial parts of Cuphea carthagenensis (Jacq.) J.F. Macbride (Lythraceae) are traditionally employed in Brazil to treat cardiovascular diseases. The aim of this study was to compare preparations of C. carthagenensis aerial parts (aqueous and ethanol extracts, together with derived fractions) with regard to their total phenolic contents and in vitro vasodilating activity. The main flavonoids found in the extracts were isolated and identified as quercetin derivatives. The extracts and fractions showed similar HPLC profiles with the presence of quercetin-5-O-β-glucopyranoside, quercetin-3-O-α-arabinofuranoside and quercetin-3-sulfate in all of them, but marked differences in the contents of flavonoids, proanthocyanidins, tannis and total phenolics. Excepting the aqueous extract, all assayed preparations elicited vasodilatation on pre-contracted rat aortic rings in the range of pIC(50) 4.53±0.03 to 4.98±0.06. Polynomial regression analysis demonstrated the relationship between vasodilating activity and the contents of flavonoids (r(2)=0.5190), proanthocyanidins (r(2)=0.8016), tannins (r(2)=0.8041) and total phenolics (r(2)=0.6226), suggesting the participation of these compounds in the pharmacological effect and their potential use as chemical markers for the species.
authors: Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31.270-901 Belo Horizonte, Brazil.
authors: Patricia Baier
authors: PB
authors: Krepsky
authors: Raquel Geralda
authors: RG
authors: Isidório
authors: José Dias
authors: JD
authors: de Souza Filho
authors: Steyner França
authors: SF
authors: Côrtes
authors: Fernão Castro
authors: FC
authors: Braga
mesh_terms: D000818:Animals; D001089:Arabinose / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D031562:Cuphea / Q000737:chemistry*; D004357:Drug Synergism; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000302:isolation & purification / Q000494:pharmacology; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D013634:Tannins / Q000302:isolation & purification / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D005419:Flavonoids; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D044945:Proanthocyanidins; D013634:Tannins; C531389:quercetin 3'-sulfate; C000590953:quercetin-3-O-alpha-arabinofuranoside; C000590951:quercetin-5-O-beta-glucopyranoside; D011794:Quercetin; D001089:Arabinose