title: Honey as an antimicrobial agent against pseudomonas aeruginosa isolated from infected wounds.
abstract: BACKGROUND
  As natural products garner attention in the medical field due to emergence of antibiotic resistant strains of bacteria, honey is valued for its antibacterial activity.
  
  
  OBJECTIVE
  Fifty strains of Pseudomonas aeruginosa isolated from infected wounds were evaluated for their antibacterial action using honey in comparison with different antibiotics and Dettol.
  
  
  RESULTS
  All the strains were found to be sensitive to honey at a minimum inhibitory concentration of 20% in comparison with Dettol at 10% using agar dilution method. In the second step, the time kill assay was performed on five isolates of P. aeruginosa to demonstrate the bactericidal activity of honey at different dilutions of honey ranging from 20% to 100% at regular time intervals. All the isolates of P. aeruginosa tested were killed in 12-24 h depending on the dilutions of the honey tested. Thus, honey could prevent the growth of P. aeruginosa even if it was diluted by deionized water by fivefolds in vitro. Honey had almost uniform bactericidal activity against P. aeruginosa irrespective of their susceptibility to different classes of antibiotics.
  
  
  CONCLUSIONS
  Honey which is a natural, non-toxic, and an inexpensive product has activity against the P. aeruginosa isolated from infected wounds may make it an alternative topical choice in the treatment of wound infections.
authors: Department of Microbiology, Kasturba Medical College, Manipal University, Manipal, India.
authors: Vishnu Prasad
authors: VP
authors: Shenoy
authors: Mamatha
authors: M
authors: Ballal
authors: Pg
authors: P
authors: Shivananda
authors: Indira
authors: I
authors: Bairy