title: Molecular characterization of the porcine TEAD3 (TEF-5) gene: examination of a promoter mutation as the causal mutation of a quantitative trait loci affecting the androstenone level in boar fat.
abstract: A quantitative trait loci (QTL) for accumulation of androstenone in fat has been identified in an Large White × Meishan cross in a region of SSC7-containing TEAD3. In humans, TEAD3 is a transcription activator, known to be able to regulate the transcription of HSD3B. This enzyme is involved in the degradation of androstenone in the liver. In this study, porcine transcripts of TEAD3 were characterized and compared with mammalian transcripts. The complete structure of porcine TEAD3 gene was characterized including two 5' non-coding exons and one exon 5 not used in porcine transcripts. Variations were screened in sequences related to TEAD3: in exons, in flanking sequences of exons and in the promoter region. A SNP characterized at 726 bp at 5' of the first exon was tested on several pig populations without coherent and convincing results concerning its association with androstenone levels. We showed that in the liver of adult boars, the transcripts levels of TEAD3 and HSD3B were correlated. As in humans, it is possible that HSD3B is a target gene of TEAD3 in porcine liver. Nevertheless, no expression variation was observed for TEAD3 or HSD3B in liver between animals with different genotypes at the SNP. We concluded that this SNP was not the causal mutation of this QTL.
authors: INRA, Laboratoire de Génétique Cellulaire, Castanet-Tolosan, France. annie.robic@toulouse.inra.fr
authors: A
authors: A
authors: Robic
authors: C
authors: C
authors: Larzul
authors: E
authors: E
authors: Grindflek
authors: P
authors: P
authors: Chevillon
authors: A
authors: A
authors: Hofer
authors: K
authors: K
authors: Fève
authors: N
authors: N
authors: Iannuccelli
authors: D
authors: D
authors: Milan
authors: A
authors: A
authors: Prunier
authors: J
authors: J
authors: Riquet
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000736:Androstenes / Q000378:metabolism*; D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D060005:Genotyping Techniques; D008099:Liver / Q000201:enzymology; D008297:Male; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D011375:Progesterone Reductase / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*; D013256:Steroids / Q000378:metabolism*; D013552:Swine / Q000235:genetics* / Q000378:metabolism; D013737:Testis / Q000201:enzymology; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D000736:Androstenes; D013256:Steroids; D014157:Transcription Factors; C000214:androst-16-en-3-one; D011375:Progesterone Reductase