title: Novel approach for optimization of a 'difficult' peptide synthesis by utilizing quantitative reaction monitoring assays.
abstract: Three different synthetic strategies were tested to synthesize EGFRvIII peptide (1), which is a functional variant of the epidermal growth factor receptor, a protein that has been well validated as a target for cancer therapy. The initial synthesis was performed with Applied Biosystem (Carlsbad, CA, USA) 433A peptide synthesizer and it indicated that the last three amino acids coupling and Fmoc removal rates were lower than the rest of the sequence. Purity of the crude peptide was 54.5%. The second synthesis was performed manually utilizing C S Bio (Menlo Park, CA, USA) synthesizer and the Kaiser test for reaction monitoring. Because of non-optimized reaction conditions and an unexpected by-product, lower purity crude peptide (40.5%) was obtained. Quantitative assays to monitor reactions were developed and demonstrated in gram scale synthesis with C S Bio synthesizer. The optimized synthetic conditions improved the peptide purity to 68.1%.
authors: Department of Bioprocess Research and Development, Pfizer Global Biologics, Chesterfield, MO 63017, USA. jari.finneman@pfizer.com
authors: Jari I
authors: JI
authors: Finneman
authors: Mark J
authors: MJ
authors: Pozzo
mesh_terms: D066246:ErbB Receptors / Q000737:chemistry; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D010455:Peptides; D066246:ErbB Receptors