title: Genomic and antigenic characterization of the newly emerging Chinese duck egg-drop syndrome flavivirus: genomic comparison with Tembusu and Sitiawan viruses.
abstract: Duck egg-drop syndrome virus (DEDSV) is a newly emerging pathogenic flavivirus causing avian diseases in China. The infection occurs in laying ducks characterized by a severe drop in egg production with a fatality rate of 5-15 %. The virus was found to be most closely related to Tembusu virus (TMUV), an isolate from mosquitoes in South-east Asia. Here, we have sequenced and characterized the full-length genomes of seven DEDSV strains, including the 5'- and 3'-non-coding regions (NCRs). We also report for the first time the ORF sequences of TMUV and Sitiawan virus (STWV), another closely related flavivirus isolated from diseased chickens. We analysed the phylogenetic and antigenic relationships of DEDSV in relation to the Asian viruses TMUV and STWV, and other representative flaviviruses. Our results confirm the close relationship between DEDSV and TMUV/STWV and we discuss their probable evolutionary origins. We have also characterized the cleavage sites, potential glycosylation sites and unique motifs/modules of these viruses. Additionally, conserved sequences in both 5'- and 3'-NCRs were identified and the predicted secondary structures of the terminal sequences were studied. Antigenic cross-reactivity comparisons of DEDSV with related pathogenic flaviviruses identified a surprisingly close relationship with dengue virus (DENV) and raised the question of whether or not DEDSV may have a potential infectious threat to man. Importantly, DEDSV can be efficiently recognized by a broadly cross-reactive flavivirus mAb, 2A10G6, derived against DENV. The significance of these studies is discussed in the context of the emergence, evolution, epidemiology, antigenicity and pathogenicity of the newly emergent DEDSV.
authors: Graduate University, Chinese Academy of Sciences, Beijing, PR China.|CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beichen West Road, Beijing, PR China.
authors: Peipei
authors: P
authors: Liu
authors: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beichen West Road, Beijing, PR China.
authors: Hao
authors: H
authors: Lu
authors: Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
authors: Shuang
authors: S
authors: Li
authors: UMR 190 'Emergence de Pathologies Virales' (AMU-IRD-EHESP), Aix-Marseille Université and IHU Mediterranee-infection, Marseille, France.
authors: Gregory
authors: G
authors: Moureau
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.
authors: Yong-Qiang
authors: YQ
authors: Deng
authors: Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
authors: Yongyue
authors: Y
authors: Wang
authors: Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
authors: Lijiao
authors: L
authors: Zhang
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.
authors: Tao
authors: T
authors: Jiang
authors: UMR 190 'Emergence de Pathologies Virales' (AMU-IRD-EHESP), Aix-Marseille Université and IHU Mediterranee-infection, Marseille, France.
authors: Xavier
authors: X
authors: de Lamballerie
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.
authors: Cheng-Feng
authors: CF
authors: Qin
authors: NERC, CEH Wallingford, Maclean Building, Benson Lane, Crowmarsh Gifford, Wallingford, Oxfordshire, OX10 8BB UK.|UMR 190 'Emergence de Pathologies Virales' (AMU-IRD-EHESP), Aix-Marseille Université and IHU Mediterranee-infection, Marseille, France.
authors: Ernest A
authors: EA
authors: Gould
authors: Graduate University, Chinese Academy of Sciences, Beijing, PR China.
authors: Jingliang
authors: J
authors: Su
authors: Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Lincui East Road, Beijing, PR China.|National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, PR China.|Graduate University, Chinese Academy of Sciences, Beijing, PR China.|CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beichen West Road, Beijing, PR China.
authors: George F
authors: GF
authors: Gao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D002681:China; D003429:Cross Reactions; D003545:Cysteine / Q000235:genetics; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology; D004372:Ducks / Q000276:immunology / Q000821:virology; D005416:Flavivirus / Q000235:genetics* / Q000276:immunology*; D018177:Flavivirus Infections / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral; D006031:Glycosylation; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology / Q000821:virology*; D017422:Sequence Analysis, DNA
chemical_list: D000941:Antigens; D003545:Cysteine