title: Development and validation of a bioanalytical LC-UV method with solid-phase extraction for determination of valproic acid in saliva.
abstract: A bioanalytical HPLC method with UV detection for the determination of the antiepileptic drug valproic acid in human saliva has been developed and validated. Saliva represents an alternative matrix for therapeutic monitoring of antiepileptic drugs due to the increasing interest in free drug concentration. The proposed method involved solid-phase extraction for sample preparation and yielded very good mean recoveries of 99.4 % and 97.9 % for valproic acid and IS, respectively. The calibration function for valproic acid was linear over the concentration range of 1.0-50.0 μg mL⁻¹ (R² = 0.9989). Within-run and between-run precision and accuracy were studied at four concentrations and RSDs were less than 7.3 and 2.2 %, while accuracy values were higher than 96.8 and 97.5 %, respectively. The described method provides sensitivity, linearity, precision, accuracy and is suitable for analyses of valproic acid in saliva samples.
authors: Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, University »Ss Cyril and Methodius«, Skopje, Macedonia. jasminatonic@yahoo.com
authors: Jasmina
authors: J
authors: Tonic-Ribarska
authors: Arlinda
authors: A
authors: Haxhiu
authors: Zoran
authors: Z
authors: Sterjev
authors: Gordana
authors: G
authors: Kiteva
authors: Ljubica
authors: L
authors: Suturkova
authors: Suzana
authors: S
authors: Trajkovic-Jolevska
mesh_terms: D000293:Adolescent; D000328:Adult; D000927:Anticonvulsants / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use; D001702:Biopharmaceutics / Q000379:methods*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004305:Dose-Response Relationship, Drug; D016903:Drug Monitoring / Q000379:methods; D004355:Drug Stability; D004827:Epilepsy / Q000188:drug therapy / Q000378:metabolism; D006801:Humans; D057230:Limit of Detection; D008875:Middle Aged; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D052616:Solid Phase Extraction; D013056:Spectrophotometry, Ultraviolet; D014635:Valproic Acid / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use; D055815:Young Adult
chemical_list: D000927:Anticonvulsants; D014635:Valproic Acid