title: Fast and precise algorithm based on maximum radial symmetry for single molecule localization.
abstract: We present an algorithm to estimate the location of single fluorescent molecule with both high speed and high precision. This algorithm is based on finding the subpixel position with maximum radial symmetry in a pixelated single molecule fluorescence image. Compared with conventional algorithms, this algorithm does not rely on point-spread-function or noise model. Through numerical simulation and experimental analysis, we found that this algorithm exhibits localization precision very close to the maximum likelihood estimator (MLE), while executes ∼1000 times faster than the MLE and ∼6 times faster than the fluoroBancroft algorithm.
authors: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China.
authors: Hongqiang
authors: H
authors: Ma
authors: Fan
authors: F
authors: Long
authors: Shaoqun
authors: S
authors: Zeng
authors: Zhen-Li
authors: ZL
authors: Huang
mesh_terms: D000465:Algorithms; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes