title: Supporting regenerative medicine by integrative dimensionality reduction.
abstract: OBJECTIVE
  The assessment of the developmental potential of stem cells is a crucial step towards their clinical application in regenerative medicine. It has been demonstrated that genome-wide expression profiles can predict the cellular differentiation stage by means of dimensionality reduction methods. Here we show that these techniques can be further strengthened to support decision making with i) a novel strategy for gene selection; ii) methods for combining the evidence from multiple data sets.
  
  
  METHODS
  We propose to exploit dimensionality reduction methods for the selection of genes specifically activated in different stages of differentiation. To obtain an integrated predictive model, the expression values of the selected genes from multiple data sets are combined. We investigated distinct approaches that either aggregate data sets or use learning ensembles.
  
  
  RESULTS
  We analyzed the performance of the proposed methods on six publicly available data sets. The selection procedure identified a reduced subset of genes whose expression values gave rise to an accurate stage prediction. The assessment of predictive accuracy demonstrated a high quality of predictions for most of the data integration methods presented.
  
  
  CONCLUSIONS
  The experimental results highlighted the main potentials of proposed approaches. These include the ability to predict the true staging by combining multiple training data sets when this could not be inferred from a single data source, and to focus the analysis on a reduced list of genes of similar predictive performance.
authors: Centre for Tissue Engineering, University of Pavia, Pavia, Italy.
authors: F
authors: F
authors: Mulas
authors: L
authors: L
authors: Zagar
authors: B
authors: B
authors: Zupan
authors: R
authors: R
authors: Bellazzi
mesh_terms: D000465:Algorithms; D003661:Decision Support Techniques; D015870:Gene Expression; D006801:Humans; D008490:Medical Informatics / Q000379:methods*; D015233:Models, Statistical; D039904:Pluripotent Stem Cells; D025341:Principal Component Analysis / Q000379:methods; D044968:Regenerative Medicine / Q000379:methods*