title: Towards a high-throughput label-free detection system combining localized-surface plasmon resonance and microfluidics.
abstract: This work reports an integrated platform combining localized-surface plasmon resonance (LSPR) and microfluidic chips to carry out multiplexed and label-free protein analysis. We fabricated an optical bench to enable detection using only a laboratory UV-Vis spectrophotometer. This assay not only improves throughput, but also allows quantitative analysis.
authors: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190, China.
authors: Yi
authors: Y
authors: Zhang
authors: Yunfang
authors: Y
authors: Tang
authors: Yi-Heui
authors: YH
authors: Hsieh
authors: Chuen-Yuan
authors: CY
authors: Hsu
authors: Jianzhong
authors: J
authors: Xi
authors: Kuan-Jiuh
authors: KJ
authors: Lin
authors: Xingyu
authors: X
authors: Jiang
mesh_terms: D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D015700:HIV Envelope Protein gp41 / Q000276:immunology; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
chemical_list: D000906:Antibodies; D015700:HIV Envelope Protein gp41; D011092:Polyethylene Glycols; D001710:Biotin; D006046:Gold