title: Alteration of leaf metabolism in Bt-transgenic rice (Oryza sativa L.) and its wild type under insecticide stress.
abstract: Insecticide is always used to control the damage from pests, while the potential influence on plants is rarely known. Time-course metabolic changes of wild and Bacillus thuringiensis (Bt) transgenic rice (Oryza sativa L.) plants after insecticide treatment were investigated by using gas chromatography-mass spectrometry (GC-MS). A combined statistical strategy of 2-way ANOVA and multivariate analyses (principal component analysis and hierarchal cluster analysis) was performed to find the stress-associated effects. The results reveal that a wide range of metabolites were dynamically varied in both varieties as a response to insecticide, in multiple metabolic pathways, such as biosynthesis and metabolism of amino acids, carbohydrates, fatty acids, TCA cycle, and the shikimate/phenylpropanoid pathway, and most of the changes were correlated with the exposure time and dependent on the variety. A set of stress defenses were activated, including phytohormone signaling pathway, antioxidant defense system, shikimate-mediated secondary metabolism, and so on. In particular, insecticide led to much stronger regulations of signaling molecules (salicylate and the precursor of jasmonate) and antioxidants (α-tocopherol and dehydroascorbate/ascorbate) in Bt-transgenic variety at the early stage. Our results demonstrated that the Bt-transgenic rice had a more acute and drastic response to insecticide stress than its non-transgenic counterpart in antioxidant system and signaling regulation.
authors: CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Jia
authors: J
authors: Zhou
authors: Lei
authors: L
authors: Zhang
authors: Yuwei
authors: Y
authors: Chang
authors: Xin
authors: X
authors: Lu
authors: Zhen
authors: Z
authors: Zhu
authors: Guowang
authors: G
authors: Xu
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001413:Bacillus thuringiensis / Q000235:genetics; D016000:Cluster Analysis; D004117:Dimethoate / Q000031:analogs & derivatives* / Q000494:pharmacology; D005798:Genes, Bacterial; D007306:Insecticides / Q000494:pharmacology*; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D055442:Metabolome / Q000187:drug effects; D015999:Multivariate Analysis; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D025341:Principal Component Analysis; D011720:Pyrazoles / Q000494:pharmacology*; D015398:Signal Transduction; D013312:Stress, Physiological; D013829:Thiadiazines / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D007306:Insecticides; D011720:Pyrazoles; D013829:Thiadiazines; C002302:dimethoxon; C083997:buprofezin; C082360:fipronil; D004117:Dimethoate