title: DNA interactions and cytotoxic studies of cis-platin analogues of substituted 2,2'-bipyridines.
abstract: Platinum(II) complexes [Pt(4″-fpbpy)Cl(2)] (1), [Pt(4″-mepbpy)Cl(2)] (2), [Pt(4″-mpbpy)Cl(2)] (3) and [Pt(4″-bopbpy)Cl(2)] (4) {where 4″-fpbpy=4-(4″-fluorophenyl)-6-phenyl-2,2'-bipyridine, 4″-mepbpy=4-(4″-methylphenyl)-6-phenyl-2,2'-bipyridine, 4″-mpbpy=4-(4″-methoxyphenyl)-6-phenyl-2,2'-bipyridine, 4″-bopbpy=4-(4″-benzyloxyphenyl)-6-phenyl-2,2'-bipyridine} have been synthesized and characterized. The binding strength and binding mode of the complexes with HS DNA (Herring Sperm) have been investigated by absorption titration and viscosity measurement studies. The results have been revealed that the complexes bind to DNA by covalent mode with intrinsic binding constant K(b) ranging from 6.05×10(4)M(-1) to 3.48×10(5)M(-1). The unwinding angle of pUC19 DNA has been evaluated by gel electrophoresis assay. The brine shrimp bioassay has been performed to study the in vitro cytotoxic properties of the synthesized metal complexes.
authors: Department of Chemistry, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India. jeenen@gmail.com
authors: Mohan N
authors: MN
authors: Patel
authors: Pradhuman A
authors: PA
authors: Parmar
authors: Deepen S
authors: DS
authors: Gandhi
authors: Anshul P
authors: AP
authors: Patidar
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D000042:Absorption; D000818:Animals; D001156:Artemia / Q000166:cytology / Q000187:drug effects; D001681:Biological Assay; D016923:Cell Death / Q000187:drug effects; D002945:Cisplatin / Q000378:metabolism* / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D004583:Electrons; D060328:Magnetic Phenomena; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry; D013057:Spectrum Analysis; D013818:Thermogravimetry; D017186:Titrimetry; D014783:Viscosity / Q000187:drug effects
chemical_list: D015082:2,2'-Dipyridyl; D004247:DNA; D002945:Cisplatin