title: Discrimination of different genuine Danshen and their extracts by Fourier transform infrared spectroscopy combined with two-dimensional correlation infrared spectroscopy.
abstract: In this study, six varieties of Danshen from different populations and genuine ("Daodi" in Chinese transliteration) regions were discriminated and identified by a three-step infrared spectroscopy method (Fourier transform-infrared spectroscopy (FT-IR) coupled with second derivative infrared spectroscopy (SD-IR) and two dimensional correlation infrared spectroscopy (2D-IR)). Though only small differences were found among the FT-IR spectra of the six Danshen samples, the positions and intensities of peaks at 3393, 3371, 1613, 1050, and 1,036 cm(-1) could be considered as the key factors to discriminate them. More significant differences were exhibited in their SD-IR, particularly for the peaks around 1080, 1144, 695, 665, 800, 1610, 1510, 1450, 1117 and 1,077 cm(-1). The visual 2D-IR spectra provided dynamic chemical structure information of the six Danshen samples with presenting different particular auto-peak clusters, respectively. Moreover, the contents of salvianolic acid B in all samples were measured quantitatively by a validated ultra performance liquid chromatography (UPLC), which was consistent with the FT-IR findings. This study provides a promising method for characteristics and quality control of the complicated and extremely similar herbal medicine like Danshen, which is more cost effective and time saving.
authors: Shenyang Pharmaceutical University, Shenyang Liaoning 110016, PR China.
authors: Xin-Hu
authors: XH
authors: Liu
authors: Chang-Hua
authors: CH
authors: Xu
authors: Su-Qin
authors: SQ
authors: Sun
authors: Jian
authors: J
authors: Huang
authors: Ke
authors: K
authors: Zhang
authors: Guo-Yu
authors: GY
authors: Li
authors: Yun
authors: Y
authors: Zhu
authors: Qun
authors: Q
authors: Zhou
authors: Zhi-Cheng
authors: ZC
authors: Zhang
authors: Jin-Hui
authors: JH
authors: Wang
mesh_terms: D000475:Alkenes / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis*; D059808:Polyphenols / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000475:Alkenes; D010936:Plant Extracts; D059808:Polyphenols; C568740:salvianolic acid