title: Effect of Mitofusin 2 on smooth muscle cells proliferation in hypoxic pulmonary hypertension.
abstract: Mitofusin 2 (Mfn2) is an important mitochondrial protein in maintaining mitochondrial network and bioenergetics. Recently, Mfn2 has been reported to have a potential role in regulating cell proliferation, apoptosis, and differentiation in many cell types. In this study, we performed immunohistochemistry, pulmonary artery smooth muscle cells (PASMCs) DNA analysis, proliferating cell nuclear antigen expression and cell cycle analysis to determine the role of Mfn2 in hypoxia-induced pulmonary vascular remodeling. Our results showed that hypoxia promoted the proliferation of pulmonary artery smooth muscle cells, including regulating more cells at G(2)/M+S phase, increasing proliferating cell nuclear antigen and Cyclin A expression, whereas all these effects of hypoxia were suppressed after the cells were treated with siRNA against Mfn2. Our results also proved that PI3K/Akt signaling pathway was involved in Mfn2-induced smooth muscle cell proliferation. Thus, these results indicate that Mfn2 mediates PASMC proliferation in hypoxic pulmonary hypertension via the PI3K/Akt signaling pathway.
authors: Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University (Daqing), Daqing, Heilongjiang Province, PR China.
authors: Dandan
authors: D
authors: Zhang
authors: Cui
authors: C
authors: Ma
authors: Shanshan
authors: S
authors: Li
authors: Yajuan
authors: Y
authors: Ran
authors: Jianguo
authors: J
authors: Chen
authors: Ping
authors: P
authors: Lu
authors: Shuai
authors: S
authors: Shi
authors: Daling
authors: D
authors: Zhu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle; D049109:Cell Proliferation; D019925:Cyclin A / Q000096:biosynthesis; D004247:DNA / Q000378:metabolism; D020558:GTP Phosphohydrolases; D006976:Hypertension, Pulmonary / Q000378:metabolism*; D000860:Hypoxia; D008168:Lung / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D024101:Mitochondrial Proteins / Q000096:biosynthesis*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
chemical_list: D019925:Cyclin A; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D018809:Proliferating Cell Nuclear Antigen; D034741:RNA, Small Interfering; D013778:Tetrazolium Salts; D013844:Thiazoles; D004247:DNA; D020558:GTP Phosphohydrolases; C489710:Mfn2 protein, rat; C022616:thiazolyl blue