title: A novel linkage map of sugarcane with evidence for clustering of retrotransposon-based markers.
abstract: BACKGROUND
  The development of sugarcane as a sustainable crop has unlimited applications. The crop is one of the most economically viable for renewable energy production, and CO2 balance. Linkage maps are valuable tools for understanding genetic and genomic organization, particularly in sugarcane due to its complex polyploid genome of multispecific origins. The overall objective of our study was to construct a novel sugarcane linkage map, compiling AFLP and EST-SSR markers, and to generate data on the distribution of markers anchored to sequences of scIvana_1, a complete sugarcane transposable element, and member of the Copia superfamily.
  
  
  RESULTS
  The mapping population parents ('IAC66-6' and 'TUC71-7') contributed equally to polymorphisms, independent of marker type, and generated markers that were distributed into nearly the same number of co-segregation groups (or CGs). Bi-parentally inherited alleles provided the integration of 19 CGs. The marker number per CG ranged from two to 39. The total map length was 4,843.19 cM, with a marker density of 8.87 cM. Markers were assembled into 92 CGs that ranged in length from 1.14 to 404.72 cM, with an estimated average length of 52.64 cM. The greatest distance between two adjacent markers was 48.25 cM. The scIvana_1-based markers (56) were positioned on 21 CGs, but were not regularly distributed. Interestingly, the distance between adjacent scIvana_1-based markers was less than 5 cM, and was observed on five CGs, suggesting a clustered organization.
  
  
  CONCLUSIONS
  Results indicated the use of a NBS-profiling technique was efficient to develop retrotransposon-based markers in sugarcane. The simultaneous maximum-likelihood estimates of linkage and linkage phase based strategies confirmed the suitability of its approach to estimate linkage, and construct the linkage map. Interestingly, using our genetic data it was possible to calculate the number of retrotransposon scIvana_1 (~60) copies in the sugarcane genome, confirming previously reported molecular results. In addition, this research possibly will have indirect implications in crop economics e.g., productivity enhancement via QTL studies, as the mapping population parents differ in response to an important fungal disease.
authors: Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, 13418-900, Piracicaba, Brazil.
authors: Alessandra C
authors: AC
authors: Palhares
authors: Taislene B
authors: TB
authors: Rodrigues-Morais
authors: Marie-Anne
authors: MA
authors: Van Sluys
authors: Douglas S
authors: DS
authors: Domingues
authors: Walter
authors: W
authors: Maccheroni
authors: Hamilton
authors: H
authors: Jordão
authors: Anete P
authors: AP
authors: Souza
authors: Thiago G
authors: TG
authors: Marconi
authors: Marcelo
authors: M
authors: Mollinari
authors: Rodrigo
authors: R
authors: Gazaffi
authors: Antonio Augusto F
authors: AA
authors: Garcia
authors: Maria Lucia Carneiro
authors: ML
authors: Vieira
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D002874:Chromosome Mapping / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D008040:Genetic Linkage; D005819:Genetic Markers; D005810:Multigene Family; D018626:Retroelements; D031786:Saccharum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D018626:Retroelements