title: Can platelet BACE1 levels be used as a biomarker for Alzheimer's disease? Proof-of-concept study.
abstract: To date there is no validated peripheral biomarker to assist with the clinical diagnosis of Alzheimer's disease (AD). Platelet proteins have been studied as AD biomarkers with relative success. In this study, we investigated whether platelet BACE1 levels differ between AD and cognitively normal (CN) control patients. Using a newly developed ELISA method, we found that BACE1 levels were significantly lower in AD compared to CN subjects. These data were supported by the observation that several BACE1 isoforms, identified by Western blotting, were also lower in AD platelets. This proof-of-concept study provides evidence for testing platelet BACE1 levels as a peripheral AD biomarker using a novel, sensitive and inexpensive method.
authors: Haldeman Laboratory of Molecular Diagnostics and Therapeutics, Banner Sun Health Research Institute, Sun City, AZ 85351, USA.
authors: Boris
authors: B
authors: Decourt
authors: Aaron
authors: A
authors: Walker
authors: Amanda
authors: A
authors: Gonzales
authors: Michael
authors: M
authors: Malek-Ahmadi
authors: Carolyn
authors: C
authors: Liesback
authors: Kathryn J
authors: KJ
authors: Davis
authors: Christine M
authors: CM
authors: Belden
authors: Sandra A
authors: SA
authors: Jacobson
authors: Marwan N
authors: MN
authors: Sabbagh
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000175:diagnosis* / Q000378:metabolism*; D053829:Amyloid Precursor Protein Secretases / Q000378:metabolism*; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism*; D015415:Biomarkers; D001792:Blood Platelets / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D020033:Protein Isoforms
chemical_list: D015415:Biomarkers; D020033:Protein Isoforms; D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; C509280:BACE1 protein, human