title: TRPC5 channel is the mediator of neurotrophin-3 in regulating dendritic growth via CaMKIIα in rat hippocampal neurons.
abstract: Neurotrophin-3 (NT-3) plays numerous important roles in the CNS and the elevation of intracellular Ca(2+) ([Ca(2+)](i)) is critical for these functions of NT-3. However, the mechanism by which NT-3 induces [Ca(2+)](i) elevation remains largely unknown. Here, we found that transient receptor potential canonical (TRPC) 5 protein and TrkC, the NT-3 receptor, exhibited a similar temporal expression in rat hippocampus and cellular colocalization in hippocampal neurons. Stimulation of the neurons by NT-3 induced a nonselective cation conductance and PLCγ-dependent [Ca(2+)](i) elevation, which were both blocked when TRPC5, but not TRPC6 channels, were inhibited. Moreover, the Ca(2+) influx through TRPC5 induced by NT-3 inhibited the neuronal dendritic growth through activation of calmodulin-dependent kinase (CaMK) IIα. In contrast, the Ca(2+) influx through TRPC6 induced by NT-4 promoted the dendritic growth. Thus, TRPC5 acts as a novel and specific mediator for NT-3 to regulate dendrite development through CaMKIIα.
authors: Institute of Neuroscience and State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors: Zhuohao
authors: Z
authors: He
authors: Caixia
authors: C
authors: Jia
authors: Shengjie
authors: S
authors: Feng
authors: Kechun
authors: K
authors: Zhou
authors: Yilin
authors: Y
authors: Tai
authors: Xue
authors: X
authors: Bai
authors: Yizheng
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000502:physiology*; D003712:Dendrites / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006624:Hippocampus / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008959:Models, Neurological; D009474:Neurons / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D020933:Neurotrophin 3 / Q000378:metabolism* / Q000502:physiology; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D050052:TRPC Cation Channels / Q000235:genetics / Q000502:physiology*
chemical_list: D020933:Neurotrophin 3; D050052:TRPC Cation Channels; C503500:Trpc5 protein, rat; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; C517675:Camk2a protein, rat