title: Association of HPV infections with second primary tumors in early-staged oral cavity cancer.
abstract: OBJECTIVE
  The infection of human papilloma virus (HPV) has been reported in head and neck cancer; however, the clinical significance of HPV infection on the pathogenesis of oral cavity squamous cell carcinoma (OSCC) is still uncertain.
  
  
  METHODS
  The study recruited 103 patients with pathological early-stage OSCC between March 1997 and December 2003 from Chang Gung Memorial Hospital, Taiwan. Tumor specimens were HPV-genotyped by the EasychipVR HPV Blot method. Clinical association study was performed by using chi-square, Kaplan-Meier, and logrank tests.
  
  
  RESULTS
  Thirty-one patients (30.1%) were positive for HPV infection. The most frequent HPV types were types 16 (16 patients, 51.6%) and 18 (seven patients, 22.6%). HPV infection was not associated with tumor aggressiveness (pathological tumor stage or differentiation status), risk exposure (alcohol, cigarette, or areca quid chewing habit), or the treatment outcome (disease-free survival or overall survival). However, infection with HPV-18 was associated with the occurrence of a second primary cancers (P = 0.033), indicating the infection of HPV in OSCC enhances the susceptibility of developing secondary malignancy.
  
  
  CONCLUSIONS
  There are 30% of the patients with OSCC infected with HPV, with most high-risk types. HPV-18 infection may enhance the susceptibility of second primary tumors. Large scale of validation study will be needed to confirm this result.
authors: Department of Otorhinolaryngology, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors: S F
authors: SF
authors: Huang
authors: H F
authors: HF
authors: Li
authors: C T
authors: CT
authors: Liao
authors: H M
authors: HM
authors: Wang
authors: I H
authors: IH
authors: Chen
authors: J T C
authors: JT
authors: Chang
authors: Y J
authors: YJ
authors: Chen
authors: A J
authors: AJ
authors: Cheng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000428:Alcohol Drinking; D052685:Alphapapillomavirus / Q000145:classification / Q000502:physiology*; D001114:Areca; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D004198:Disease Susceptibility; D018572:Disease-Free Survival; D005260:Female; D005500:Follow-Up Studies; D052162:Human papillomavirus 16 / Q000502:physiology; D052161:Human papillomavirus 18 / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000821:virology*; D020360:Neoadjuvant Therapy; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D016609:Neoplasms, Second Primary / Q000821:virology*; D030361:Papillomavirus Infections / Q000821:virology*; D012189:Retrospective Studies; D012907:Smoking; D015996:Survival Rate; D016896:Treatment Outcome