title: "Bam," a novel glucocorticoid-induced BH3-only transcript from the BCL2L11/Bim locus, does not appear to be translated.
abstract: Glucocorticoids (GCs) are steroid hormones that induce cell death and cell cycle arrest in lymphoid tissues. By virtue of this property, GCs are widely exploited in the therapy of acute lymphoblastic leukemia (ALL) in children. We reported a novel BH3-only transcript, "Bam," from the BCL2L11 locus, which was first described in patients with multiple myeloma. The Bam gene consists of two exons, and became of particular interest to us when we found that it was regulated in the majority of children with ALL and many in vitro systems in which GCs induce cell death. Being a BH3-only transcript, Bam retains a BH3 domain identical to that of Bim, although Bam has a unique C-terminus that is totally different from that of its relative Bim. The present work analyzes whether Bam is translated or not. Since we could not detect Bam in the endogenous situation, we evaluated its 5' untranslated region (UTR). This revealed that there are three out-of-frame initiation codons preceding the Bam open reading frame (ORF). Experiments with constructs without out-of-frame initiation codons and constructs harboring such codons in their 5' UTR revealed that Bam translation is handicapped by their presence. Moreover, there was no Kozak translational initiation sequence surrounding any of the AUGs. Taken together, results of the present study strongly suggest that this transcript is translated at a very low rate, if at all.
authors: Division of Molecular Pathophysiology, Biocenter, Medical University Innsbruck, Austria. mmansha2005@yahoo.com
authors: Muhammad
authors: M
authors: Mansha
authors: Abrar
authors: A
authors: Hussain
authors: Anita
authors: A
authors: Kofler
authors: Claudia
authors: C
authors: Grubbauer
authors: Katrin
authors: K
authors: Goetsch
authors: Christian
authors: C
authors: Ploner
authors: Reinhard
authors: R
authors: Kofler
mesh_terms: D020121:5' Untranslated Regions; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D000072224:Bcl-2-Like Protein 11; D002460:Cell Line; D018387:Codon, Initiator; D023061:Gene Order; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D020121:5' Untranslated Regions; D051017:Apoptosis Regulatory Proteins; C000606775:BCL2L11 protein, human; D000072224:Bcl-2-Like Protein 11; D018387:Codon, Initiator; D008565:Membrane Proteins; D033681:Mitochondrial Membrane Transport Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; C581062:RTL10 protein, human