title: Ethanolic extracts and isolated compounds from small-leaf grape (Vitis thunbergii var. taiwaniana) with antihypertensive activities.
abstract: This study aimed to investigate the antihypertensive effects of ethanolic extracts (EE) and compounds isolated from the small-leaf grape (Vitis thunbergii var. taiwaniana, VTT). The highest antiangiotensin-converting enzyme (anti-ACE) was found in stem-EE (IC50 was 69.5 μg/mL). In spontaneously hypertensive rats (SHRs), stem-EE effectively reduced blood pressure 24 h after administration of a single oral dose or when administered daily for 4 weeks. The isolated compounds, including (+)-vitisin A, ampelopsin C, and (+)-ε-viniferin, were shown to have anti-ACE and vasodilating effects against phenylephrine-induced tensions in an endothelium-intact aortic ring, with (+)-vitisin A being the most effective compound. Compared to control rats, SHRs showed significantly reduced systolic and diastolic blood pressures 24 h after a single oral dose of (+)-vitisin A (10 mg/kg) or captopril (2 mg/kg). These results suggest that the development of functional foods with VTT extracts may be beneficial for regulating blood pressure.
authors: School of Pharmacy, Taipei Medical University , Taipei, Taiwan.
authors: Yin-Shiou
authors: YS
authors: Lin
authors: Yeh-Lin
authors: YL
authors: Lu
authors: Guei-Jane
authors: GJ
authors: Wang
authors: Lih-Geeng
authors: LG
authors: Chen
authors: Chi-Luan
authors: CL
authors: Wen
authors: Wen-Chi
authors: WC
authors: Hou
mesh_terms: D000284:Administration, Oral; D000809:Angiotensins / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage* / Q000302:isolation & purification*; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D002216:Captopril / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D000431:Ethanol; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology; D027843:Vitis / Q000737:chemistry*
chemical_list: D000809:Angiotensins; D000959:Antihypertensive Agents; D001572:Benzofurans; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D013267:Stilbenes; C412469:vitisin A; C106407:ampelopsin; D000431:Ethanol; C091891:epsilon-viniferin; D002216:Captopril