title: Prevalence of genes encoding extracellular proteases in Staphylococcus aureus - important targets triggering immune response in vivo.
abstract: Proteases of Staphylococcus aureus have long been considered to function as important virulence factors, although direct evidence of the role of particular enzymes remains incomplete and elusive. Here, we sought to provide a collective view of the prevalence of extracellular protease genes in genomes of commensal and pathogenic strains of S. aureus and their expression in the course of human and mouse infection. Data on V8 protease, staphopains A and B, aureolysin, and the recently described and poorly characterized group of six Spl proteases are provided. A phylogenetically diverse collection of 167 clinical isolates was analyzed, resulting in the comprehensive genetic survey of the prevalence of protease-encoding genes. No correlation between identified gene patterns with specific infections was established. Humoral response against the proteases of interest was examined in the sera derived from human patients and from a model mouse infection. The analysis suggests that at least some, if not all, tested proteases are expressed and secreted during the course of infection. Overall, the results presented in this study support the hypothesis that the secretory proteases as a group may contribute to the virulence of S. aureus.
authors: Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors: Michal
authors: M
authors: Zdzalik
authors: Abdulkarim Y
authors: AY
authors: Karim
authors: Krzysztof
authors: K
authors: Wolski
authors: Pawel
authors: P
authors: Buda
authors: Kinga
authors: K
authors: Wojcik
authors: Sarah
authors: S
authors: Brueggemann
authors: Piotr
authors: P
authors: Wojciechowski
authors: Sigrun
authors: S
authors: Eick
authors: Ann-Marie
authors: AM
authors: Calander
authors: Ing-Marie
authors: IM
authors: Jonsson
authors: Malgorzata
authors: M
authors: Kubica
authors: Klaudia
authors: K
authors: Polakowska
authors: Jacek
authors: J
authors: Miedzobrodzki
authors: Benedykt
authors: B
authors: Wladyka
authors: Jan
authors: J
authors: Potempa
authors: Grzegorz
authors: G
authors: Dubin
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D051379:Mice; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D015995:Prevalence; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D037521:Virulence Factors / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D037521:Virulence Factors; D010447:Peptide Hydrolases