title: The invasion and metastasis promotion role of CD97 small isoform in gastric carcinoma.
abstract: CD97 is over-expressed in the majority of gastric adenocarcinomas and is associated with its dedifferentiation and aggressiveness. Our previous results demonstrated that out of three CD97 isoforms tested, only the small one was able to promote increased invasiveness in vitro. Based on these data we further aimed to investigate the role of CD97 small isoform in gastric cancer progression in vivo by employing the cells with a stable CD97 small isoform knock-down and an orthotopic gastric cancer mouse model. We could demonstrate that the knock down of CD97/EGF1,2,5, led to a significant decrease in the number of cells penetrating the gelatin coated membrane as compared with control cells. In the gastric cancer mouse model, both the hypodermic and the orthotopic yielded tumor masses of the CD97/EGF1,2,5kd group and were significantly smaller than the control. Metastatic tumor cell number in early metastatic regional lymph nodes on post-operative day 42 was distinctly decreased in the CD97/EGF1,2,5kd group as compared with the SGC-NS group, and was accompanied with the downregulation of CD44, VEGFR, CD31 and CD97. We concluded in this study that CD97 small isoform not only supported gastric cancer local growth, but also promoted metastatic spread in orthotopically implanted mouse model suggesting involvement of the CD97 small isoform in the preparation of (pre)metastatic niche.
authors: Department of Surgery, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People's Republic of China.
authors: Daren
authors: D
authors: Liu
authors: Bogusz
authors: B
authors: Trojanowicz
authors: Longyun
authors: L
authors: Ye
authors: Chao
authors: C
authors: Li
authors: Luqing
authors: L
authors: Zhang
authors: Xiaowen
authors: X
authors: Li
authors: Guogang
authors: G
authors: Li
authors: Yixiong
authors: Y
authors: Zheng
authors: Li
authors: L
authors: Chen
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D043562:Receptors, G-Protein-Coupled; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: C095004:ADGRE5 protein, human; D015703:Antigens, CD; D020033:Protein Isoforms; D043562:Receptors, G-Protein-Coupled