title: Increased oxidant generation in the metabolism of hypoxanthine to uric acid and endothelial dysfunction in early-onset and late-onset preeclamptic women.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the association of vascular endothelial dysfunction with increased oxidant generation in the metabolism of hypoxanthine to uric acid in early-onset compared to late-onset preeclampsia.
  
  
  METHODS
  We investigated 12 women with early-onset preeclampsia, 14 women with late-onset preeclampsia, and 20 women with uncomplicated pregnancies. We measured serum derivatives of reactive oxygen metabolites (d-ROMs) as a marker of oxygen free radicals, serum biological antioxidant potential (BAP), hypoxanthine, uric acid, uric acid clearance (CUA), and flow-mediated vasodilation (FMD) as a marker of endothelial function in preeclamptic women.
  
  
  RESULTS
  Concentration of d-ROMs was significantly higher in both preeclamptic groups compared to the control group. Plasma levels of uric acid were significantly elevated in both preeclamptic groups compared to the control group. Plasma levels of hypoxanthine were significantly higher in early-onset preeclamptic women compared to controls, but not in late-onset preeclamptic women. CUA was significantly lower in late-onset preeclamptic women compared to controls, but not in early-onset preeclamptic women. The concentrations of hypoxanthine and uric acid correlated positively with the concentration of d-ROMs in all pregnant women. FMD was significantly lower in both preeclamptic groups compared with controls, but FMD in the early-onset preeclamptic group was significantly lower than in the late-onset preeclamptic group.
  
  
  CONCLUSIONS
  We found that increased oxidant generation during metabolism of hypoxanthine to uric acid may impair endothelial function in early-onset preeclampsia.
authors: Department of Obstetrics and Gynecology, Aichi Medical University School of Medicine, Aichi, Japan. kw446433@yahoo.co.jp
authors: Kazushi
authors: K
authors: Watanabe
authors: Toshitaka
authors: T
authors: Mori
authors: Ai
authors: A
authors: Iwasaki
authors: Chiharu
authors: C
authors: Kimura
authors: Hiroshi
authors: H
authors: Matsushita
authors: Koichi
authors: K
authors: Shinohara
authors: Akihiko
authors: A
authors: Wakatsuki
mesh_terms: D000328:Adult; D017668:Age of Onset; D016022:Case-Control Studies; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D005865:Gestational Age; D006801:Humans; D019271:Hypoxanthine / Q000097:blood / Q000378:metabolism*; D016877:Oxidants / Q000097:blood / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000097:blood / Q000453:epidemiology / Q000378:metabolism* / Q000503:physiopathology*; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000097:blood / Q000378:metabolism; D012720:Severity of Illness Index; D015854:Up-Regulation; D014527:Uric Acid / Q000097:blood / Q000378:metabolism*; D055815:Young Adult
chemical_list: D016877:Oxidants; D017382:Reactive Oxygen Species; D014527:Uric Acid; D019271:Hypoxanthine