title: Raman spectroscopy and imaging: applications in human breast cancer diagnosis.
abstract: The applications of spectroscopic methods in cancer detection open new possibilities in early stage diagnostics. Raman spectroscopy and Raman imaging represent novel and rapidly developing tools in cancer diagnosis. In the study described in this paper Raman spectroscopy has been employed to examine noncancerous and cancerous human breast tissues of the same patient. The most significant differences between noncancerous and cancerous tissues were found in regions characteristic for the vibrations of carotenoids, lipids and proteins. Particular attention was paid to the role played by unsaturated fatty acids in the differentiation between the noncancerous and the cancerous tissues. Comparison of Raman spectra of the noncancerous and the cancerous tissues with the spectra of oleic, linoleic, α-linolenic, γ-linolenic, docosahexaenoic and eicosapentaenoic acids has been presented. The role of sample preparation in the determination of cancer markers is also discussed in this study.
authors: Lodz University of Technology, Institute of Applied Radiation Chemistry, Laboratory of Laser Molecular Spectroscopy, Wroblewskiego 15, 93-590 Lodz, Poland. brozek@mitr.p.lodz.pl
authors: Beata
authors: B
authors: Brozek-Pluska
authors: Jacek
authors: J
authors: Musial
authors: Radzislaw
authors: R
authors: Kordek
authors: Elena
authors: E
authors: Bailo
authors: Thomas
authors: T
authors: Dieing
authors: Halina
authors: H
authors: Abramczyk
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D001940:Breast / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014732:Vibration
chemical_list: D014408:Biomarkers, Tumor; D005227:Fatty Acids