title: Characterizing the neurite outgrowth inhibitory effect of Mani.
abstract: Mani (myelin-associated neurite-outgrowth inhibitor) protein is implicated in both axonal guidance and axonal regeneration after central nervous system (CNS) injury. Here, we applied a neurite outgrowth assay, coupled with a siRNA-driven investigation and immunocytochemistry, to unveil Mani's axonal outgrowth inhibitory effect in embryonic rat cortical primary neurons in vitro. We further demonstrate Mani's neuronal localization in comparison with a principal subunit, Cdc27, of the anaphase promoting complex (APC). Considering the protein structure of Mani obtained via a series of bio-computational studies, we propose a Cdc27-Mani-APC-related signalling pathway may be involved in CNS axon regeneration.
authors: School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.
authors: Manisha
authors: M
authors: Mishra
authors: Sachiko
authors: S
authors: Lee
authors: Michelle K
authors: MK
authors: Lin
authors: Toshihide
authors: T
authors: Yamashita
authors: Klaus
authors: K
authors: Heese
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D064191:Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism; D002478:Cells, Cultured; D055785:Gene Knockdown Techniques; D006655:Histone Deacetylases / Q000235:genetics; D053858:Metabolic Networks and Pathways; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism* / Q000648:ultrastructure; D009473:Neuronal Plasticity; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D015398:Signal Transduction; D043743:Ubiquitin-Protein Ligase Complexes / Q000378:metabolism
chemical_list: D064191:Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome; C577368:Cdc27 protein, rat; D018797:Cell Cycle Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; C559944:HDAC4 protein, rat; D006655:Histone Deacetylases