title: Preliminary characterization, antioxidant activity in vitro and hepatoprotective effect on acute alcohol-induced liver injury in mice of polysaccharides from the peduncles of Hovenia dulcis.
abstract: The fresh fleshy peduncles of Hovenia dulcis have been used as a food supplement and traditional herbal medicine for the treatment of liver diseases and alcoholic poisoning for more than a millennium. The objectives of the present study, therefore, were to determine the antioxidant activity of polysaccharides from the peduncles of H. dulcis (HDPS) and to evaluate its hepatoprotective effect on acute alcohol-induced liver injury in mice. HDPS, prepared by hot water extraction, ethanol precipitation and treatment of macroporous resin, was found to be non-starch polysaccharide and mainly composed of galactose, arabinose, rhamnose and galacturonic acid. In in vitro antioxidant assay, HDPS exhibited high superoxide radical scavenging activity, strong inhibition effect on lipid peroxidation and a medium ferrous ion-chelating activity. For hepatoprotective activity in vivo, the administration of HDPS significantly decreased the serum levels of alanine aminotransferase and aspartate aminotransferase, significantly decreased the liver level of malondialdehyde and remarkably restored the liver activities of superoxide dismutase and glutathione peroxidase in alcohol-induced liver injury mice. The results suggested that HDPS had a significant protective effect against acute alcohol-induced liver injury possibly via its antioxidant activity to protect biological systems against the oxidative stress.
authors: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors: Mingchun
authors: M
authors: Wang
authors: Peilei
authors: P
authors: Zhu
authors: Changxing
authors: C
authors: Jiang
authors: Liping
authors: L
authors: Ma
authors: Zhanjun
authors: Z
authors: Zhang
authors: Xiaoxiong
authors: X
authors: Zeng
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D000431:Ethanol / Q000009:adverse effects*; D008108:Liver Diseases, Alcoholic / Q000201:enzymology / Q000209:etiology* / Q000517:prevention & control*; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D027823:Rhamnaceae / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; D013481:Superoxides; D000431:Ethanol; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase