title: Endothelial lipase is localized to follicular epithelial cells in the thyroid gland and is moderately expressed in adipocytes.
abstract: Endothelial lipase (EL), a member of the triglyceride lipase gene family, has been shown to be a key player in HDL metabolism. Northern blots revealed that EL was highly expressed in endothelium, thyroid, lung, placenta, liver, and testis. In liver and adrenal gland, EL protein was localized with vascular endothelial cells but not parenchymal cells. EL was shown to be upregulated in tissues such as atherosclerotic plaque where it was located in macrophages, endothelial cells, and medial smooth muscle cells. The purpose of this study was to investigate the cellular localization of EL in thyroid and other tissues where EL is known to be expressed. Besides its presence in vascular endothelial and smooth muscle cells, EL protein was detected in the epithelial cells that line the follicles within the thyroid gland. EL-specific immunostaining was also found near the cell surface as well as in the cytoplasm of adipocytes. Using immunoblots, EL expression was confirmed in cultured human omental and subcutaneous adipocytes. EL expression, however, was not found in preadipocytes. These findings suggest that EL plays a role in thyroid and adipocyte biology in addition to its well-known role in endothelial function and HDL metabolism.
authors: Janssen Research and Development, Janssen Pharmaceutical Companies of Johnson and Johnson, Spring House, PA 19477, USA.
authors: Margery A
authors: MA
authors: Connelly
authors: Michael R
authors: MR
authors: D'Andrea
authors: Jenson
authors: J
authors: Qi
authors: Keli C
authors: KC
authors: Dzordzorme
authors: Bruce P
authors: BP
authors: Damiano
mesh_terms: D017667:Adipocytes / Q000201:enzymology*; D000818:Animals; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology; D004847:Epithelial Cells / Q000201:enzymology*; D006801:Humans; D007150:Immunohistochemistry; D008049:Lipase / Q000378:metabolism*; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000201:enzymology; D009852:Omentum / Q000166:cytology / Q000201:enzymology; D009928:Organ Specificity; D013045:Species Specificity; D050151:Subcutaneous Fat / Q000166:cytology / Q000201:enzymology; D013961:Thyroid Gland / Q000201:enzymology*
chemical_list: C410382:LIPG protein, human; D008049:Lipase