title: In vitro biocompatibility of solid lipid nanoparticles.
abstract: This study was undertaken to address the current deficient knowledge of cellular response to solid lipid nanoparticles (SLNs) exposure. We investigated the cytotoxicity of several SLNs formulations in two fibroblast cell lineages, Vero and MDCK. Several methods were used to explore the mechanisms involved in this cytotoxic process, including cell viability assays, flow cytometry and ROS generation assessment. Among nanoparticles tested, two of them (F4 and F5) demonstrated more cytotoxic effects in both cell lineages. The cell viability assays suggested that F4 and F5 interfere in cell mitochondrial metabolism and in lysosomal activity. In addition, F5 decreased the percentage of MDCK cells in G0/G1 and G2/M phases, with a marked increase in the Sub/G1 population, suggesting DNA fragmentation. Regarding F4, although IC(50) was higher (~700 μg/mL), this formulation affected mitochondrial membrane potential for Vero cells. However, the IC(50) of F5 was around 250 μg/mL, suggesting the effect of SDS (sodium dodecyl sulfate) present in the formulation. In summary, the nanoparticles tested here appears to be biocompatible, with the exception of F5. Further studies are required to elucidate the in vivo effects of these nanoscale structures, in order to evaluate or predict the connotation of their increased and widespread use.
authors: Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina P.O. Box 476, Florianópolis, SC, 88040-900, Brazil.
authors: Adny Henrique
authors: AH
authors: Silva
authors: Fabíola Branco
authors: FB
authors: Filippin-Monteiro
authors: Bruno
authors: B
authors: Mattei
authors: Betina G
authors: BG
authors: Zanetti-Ramos
authors: Tânia Beatiz
authors: TB
authors: Creczynski-Pasa
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival; D002522:Chlorocebus aethiops; D004285:Dogs; D005347:Fibroblasts / Q000187:drug effects*; D005434:Flow Cytometry; D008055:Lipids / Q000633:toxicity*; D061985:Madin Darby Canine Kidney Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000633:toxicity* / Q000648:ultrastructure; D017382:Reactive Oxygen Species / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014709:Vero Cells
chemical_list: D008055:Lipids; D017382:Reactive Oxygen Species