title: Biological properties of acidic cosmetic water from seawater.
abstract: This current work was to investigate the biological effects of acidic cosmetic water (ACW) on various biological assays. ACW was isolated from seawater and demonstrated several bio-functions at various concentration ranges. ACW showed a satisfactory effect against Staphylococcus aureus, which reduced 90% of bacterial growth after a 5-second exposure. We used cultured human peripheral blood mononuclear cells (PBMCs) to test the properties of ACW in inflammatory cytokine release, and it did not induce inflammatory cytokine release from un-stimulated, normal PBMCs. However, ACW was able to inhibit bacterial lipopolysaccharide (LPS)-induced inflammatory cytokine TNF-α released from PBMCs, showing an anti-inflammation potential. Furthermore, ACW did not stimulate the rat basophilic leukemia cell (RBL-2H3) related allergy response on de-granulation. Our data presented ACW with a strong anti-oxidative ability in a superoxide anion radical scavenging assay. In mass spectrometry information, magnesium and zinc ions demonstrated bio-functional detections for anti-inflammation as well as other metal ions such as potassium and calcium were observed. ACW also had minor tyrosinase and melanin decreasing activities in human epidermal melanocytes (HEMn-MP) without apparent cytotoxicity. In addition, the cell proliferation assay illustrated anti-growth and anti-migration effects of ACW on human skin melanoma cells (A375.S2) indicating that it exerted the anti-cancer potential against skin cancer. The results obtained from biological assays showed that ACW possessed multiple bioactivities, including anti-microorganism, anti-inflammation, allergy-free, antioxidant, anti-melanin and anticancer properties. To our knowledge, this was the first report presenting these bioactivities on ACW.
authors: Department of Biotechnology, Kaohsiung Medical University, 100, Shih-Chuan 1st Road, San-Ming District, Kaohsiung 807, Taiwan.
authors: Wei-Ting
authors: WT
authors: Liao
authors: Section of Microbiology, Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Kaohsiung 807, Taiwan.|Department of Medical Technology, Fooyin University, Kaohsiung County 831, Taiwan.|School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.
authors: Tsi-Shu
authors: TS
authors: Huang
authors: Department of Biotechnology, Kaohsiung Medical University, 100, Shih-Chuan 1st Road, San-Ming District, Kaohsiung 807, Taiwan.
authors: Chien-Chih
authors: 0000-0001-7307-2468
authors: CC
authors: Chiu
authors: Department of Chemical and Biochemical Engineering, Kao Yuan University, Kaohsiung 821, Taiwan.
authors: Jian-Liang
authors: JL
authors: Pan
authors: Department of Biotechnology, Kaohsiung Medical University, 100, Shih-Chuan 1st Road, San-Ming District, Kaohsiung 807, Taiwan.
authors: Shih-Shin
authors: SS
authors: Liang
authors: Department of Biotechnology, Kaohsiung Medical University, 100, Shih-Chuan 1st Road, San-Ming District, Kaohsiung 807, Taiwan.
authors: Bing-Hung
authors: BH
authors: Chen
authors: Department of Research and Development, Taiyen Biotech Co., Ltd., 15, Gong-Huan Road, Annan District, Tainan 709, Taiwan.
authors: Shi-Hui
authors: SH
authors: Chen
authors: Department of Respiratory Therapy, College of Medicine, Kaohsiung Medical University, 100, Shih-Chuan 1st Road, San-Ming District, Kaohsiung 807, Taiwan.
authors: Po-Len
authors: PL
authors: Liu
authors: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Hui-Chun
authors: HC
authors: Wang
authors: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, 70, Lien-Hai Rd, Kaohsiung 804, Taiwan.
authors: Zhi-Hong
authors: 0000-0003-4411-044X
authors: ZH
authors: Wen
authors: Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, 100, Shih-Chuan 1st Road, San-Ming District, Kaohsiung 807, Taiwan.
authors: Hui-Min
authors: 0000-0002-5162-9752
authors: HM
authors: Wang
authors: Department of Research and Development, Taiyen Biotech Co., Ltd., 15, Gong-Huan Road, Annan District, Tainan 709, Taiwan.
authors: Shu-Wen
authors: SW
authors: Hsiao
mesh_terms: D000328:Adult; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects*; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D008544:Melanocytes / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D012623:Seawater / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000975:Antioxidants