title: Tissue engineered bone grafts based on biomimetic nanocomposite PLGA/amorphous calcium phosphate scaffold and human adipose-derived stem cells.
abstract: For tissue engineering of critical size bone grafts, nanocomposites are getting more and more attractive due to their controllable physical and biological properties. We report in vitro and in vivo behaviour of an electrospun nanocomposite based on poly-lactic-co-glycolic acid and amorphous calcium phosphate nanoparticles (PLGA/a-CaP) seeded with human adipose-derived stem cells (ASC) compared to PLGA. Major findings were that cell attachment, three-dimensional ingrowth and proliferation were very good on both materials. Cell morphology changed from a spindle-shaped fibroblast-like form to a more roundish type when ASC were seeded on PLGA, while they retained their morphology on PLGA/a-CaP. Moreover, we found ASC differentiation to a phenotype committed towards osteogenesis when a-CaP nanoparticles were suspended in normal culture medium without any osteogenic supplements, which renders a-CaP nanoparticles an interesting osteoinductive component for the synthesis of other nanocomposites than PLGA/a-CaP. Finally, electrospun PLGA/a-CaP scaffold architecture is suitable for a rapid and homogenous vascularisation confirmed by a complete penetration by avian vessels from the chick chorioallantoic membrane (CAM) within one week.
authors: Division of Plastic and Hand Surgery, University Hospital Zurich, ZKF, Sternwartstrasse 14, CH-8091 Zurich, Switzerland. johanna.buschmann@usz.ch
authors: Johanna
authors: J
authors: Buschmann
authors: Luc
authors: L
authors: Härter
authors: Shuping
authors: S
authors: Gao
authors: Sonja
authors: S
authors: Hemmi
authors: Manfred
authors: M
authors: Welti
authors: Nora
authors: N
authors: Hild
authors: Oliver D
authors: OD
authors: Schneider
authors: Wendelin J
authors: WJ
authors: Stark
authors: Nicole
authors: N
authors: Lindenblatt
authors: Clement M L
authors: CM
authors: Werner
authors: Guido A
authors: GA
authors: Wanner
authors: Maurizio
authors: M
authors: Calcagni
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D001672:Biocompatible Materials; D016025:Bone Transplantation / Q000379:methods*; D002130:Calcium Phosphates / Q000737:chemistry*; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles; D010006:Osteoblasts; D010012:Osteogenesis / Q000502:physiology; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D033581:Stem Cell Transplantation; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C519480:amorphous calcium phosphate; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid