title: CYP4 mRNA expression in marine polychaete Perinereis aibuhitensis in response to petroleum hydrocarbon and deltamethrin.
abstract: A CYP4 cDNA was cloned and characterized to identify the relationship between persistent organic pollutants and stress response in marine polychaete Perinereis aibuhitensis. The full length of PaCYP4 cDNA is 1857bp and encodes 481 amino acids. The deduced amino acid sequence showed 73% identity with CYP4BB1 from polychaete Nereis virens and shared high homology to other known CYP4 sequences. The expression level of PaCYP4 under petroleum hydrocarbon (PH) and deltamethrin (DM) exposure was detected using Real-time PCR. PH and combined toxicity treatments elevated the mRNA level of PaCYP4 in a dose- and time-dependent manner. The mRNA transcripts of PaCYP4 increased at the beginning of DM exposure and then eventually decreased, and the expression level of PaCYP4 down-regulated with increasing concentration of DM. CYP4 in P. aibuhitensis plays an important role in the metabolism of petroleum hydrocarbon and organochlorine pesticide.
authors: Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China.
authors: Xue
authors: X
authors: Chen
authors: Yibing
authors: Y
authors: Zhou
authors: Dazuo
authors: D
authors: Yang
authors: Huan
authors: H
authors: Zhao
authors: Lili
authors: L
authors: Wang
authors: Xiutang
authors: X
authors: Yuan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics* / Q000378:metabolism; D006838:Hydrocarbons / Q000633:toxicity*; D008969:Molecular Sequence Data; D009570:Nitriles / Q000633:toxicity*; D010578:Petroleum / Q000633:toxicity*; D011077:Polychaeta / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D011722:Pyrethrins / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D006838:Hydrocarbons; D009570:Nitriles; D010578:Petroleum; D011722:Pyrethrins; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; C017180:decamethrin; D001189:Aryl Hydrocarbon Hydroxylases