title: Repeated injection of PEGylated solid lipid nanoparticles induces accelerated blood clearance in mice and beagles.
abstract: Surface modification of nanocarriers with amphiphilic polymer polyethylene glycol (PEG), known as PEGylation, is regarded as a major breakthrough in the application of nanocarriers. However, PEGylated nanocarriers (including liposomes and polymeric nanoparticles) induce what is referred to as the "accelerated blood clearance (ABC) phenomenon" upon repeated injection and consequently they lose their sustained circulation characteristics. Despite this, the present authors are not aware of any reports of accelerated clearance due to repeated injection for PEGylated solid lipid nanoparticles (SLNs), another promising nanocarrier. This study investigated the pharmacokinetics of PEGylated SLNs upon repeated administration in mice; moreover, the impact of circulation time on the induction of the ABC phenomenon was studied in beagles for the first time. The ABC index, selected as the ratio of the area under the concentration-time curve from time 0 to the last measured concentration of a second injection to that of the first injection, was used to evaluate the extent of this phenomenon. Results showed that the PEGylated SLNs exhibited accelerated clearance from systemic circulation upon repeated injection, both in mice and in beagles, and the ratio for the different time intervals, which showed that the ABC index exhibited significant difference within 30 minutes following the second injection, was good enough to evaluate the magnitude of ABC. This ABC index indicated that the 10 mol% PEG SLNs with a suitable prolonged circulation time induced the most marked ABC phenomenon in this research. This study demonstrated that, like PEGylated nanocarriers such as liposomes and polymeric nanoparticles, PEGylated SLNs induced the ABC phenomenon upon repeated injection--the beagle was a valuable experimental animal for this research. Furthermore, the authors considered that a relatively extended circulation time of the initial dose may be the underlying major factor determining the induction of the ABC phenomenon.
authors: College of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning, People's Republic of China.
authors: Yongxue
authors: Y
authors: Zhao
authors: Long
authors: L
authors: Wang
authors: Mina
authors: M
authors: Yan
authors: Yanling
authors: Y
authors: Ma
authors: Guangxi
authors: G
authors: Zang
authors: Zhennan
authors: Z
authors: She
authors: Yihui
authors: Y
authors: Deng
mesh_terms: D000818:Animals; D019540:Area Under Curve; D004285:Dogs; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008657:Metabolic Clearance Rate / Q000187:drug effects*; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D013154:Spleen / Q000737:chemistry / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: C099730:N-carbamylmethoxypoly(ethylene glycol)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine; D010714:Phosphatidylethanolamines; D011092:Polyethylene Glycols