title: Glycogen is the primary source of glucose during the lag phase of E. coli proliferation.
abstract: In the studies of Escherichia coli (E. coli), metabolomics analyses have mainly been performed using steady state culture. However, to analyze the dynamic changes in cellular metabolism, we performed a profiling of concentration of metabolites by using batch culture. As a first step, we focused on glucose uptake and the behavior of the first metabolite, G6P (glucose-6-phosphate). A computational formula was derived to express the glucose uptake rate by a single cell from two kinds of experimental data, extracellular glucose concentration and cell growth, being simulated by Cell Illustrator. In addition, average concentration of G6P has been measured by CE-MS. The existence of another carbon source was suggested from the computational result. After careful comparison between cell growth, G6P concentration, and the computationally obtained curve of glucose uptake rate, we predicted the consumption of glycogen in lag phase and its accumulation as an energy source in an E. coli cell for the next proliferation. We confirmed our prediction experimentally. This behavior indicates the importance of glycogen participation in the lag phase for the growth of E. coli. This article is part of a Special Issue entitled: Computational Methods for Protein Interaction and Structural Prediction.
authors: Graduate School of Science and Engineering, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8512, Japan.
authors: Tomoaki
authors: T
authors: Yamamotoya
authors: Hitomi
authors: H
authors: Dose
authors: Zhongyuan
authors: Z
authors: Tian
authors: Adrien
authors: A
authors: Fauré
authors: Yoshihiro
authors: Y
authors: Toya
authors: Masayuki
authors: M
authors: Honma
authors: Kaori
authors: K
authors: Igarashi
authors: Kenji
authors: K
authors: Nakahigashi
authors: Tomoyoshi
authors: T
authors: Soga
authors: Hirotada
authors: H
authors: Mori
authors: Hiroshi
authors: H
authors: Matsuno
mesh_terms: D019295:Computational Biology; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000032:analysis; D006003:Glycogen / Q000378:metabolism*; D050296:Microbial Viability
chemical_list: D019298:Glucose-6-Phosphate; D006003:Glycogen; D005947:Glucose