title: 13C cell wall enrichment and ionic liquid NMR analysis: progress towards a high-throughput detailed chemical analysis of the whole plant cell wall.
abstract: The ability to accurately and rapidly measure plant cell wall composition, relative monolignol content and lignin-hemicellulose inter-unit linkage distributions has become essential to efforts centered on reducing the recalcitrance of biomass by genetic engineering. Growing (13)C enriched transgenic plants is a viable route to achieve the high-throughput, detailed chemical analysis of whole plant cell wall before and after pretreatment and microbial or enzymatic utilization by (13)C nuclear magnetic resonance (NMR) in a perdeuterated ionic liquid solvent system not requiring component isolation. 1D (13)C whole cell wall ionic liquid NMR of natural abundant and (13)C enriched corn stover stem samples suggest that a high level of uniform labeling (>97%) can significantly reduce the total NMR experiment times up to ~220 times. Similarly, significant reduction in total NMR experiment time (~39 times) of the (13)C enriched corn stover stem samples for 2D (13)C-(1)H heteronuclear single quantum coherence NMR was found.
authors: BioEnergy Science Center, School of Chemistry and Biochemistry, Institute of Paper Science and Technology, Georgia Institute of Technology, 500 10th St., Atlanta, GA 30332, USA.
authors: Marcus
authors: M
authors: Foston
authors: Reichel
authors: R
authors: Samuel
authors: Arthur J
authors: AJ
authors: Ragauskas
mesh_terms: D018533:Biomass; D002247:Carbon Isotopes / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D059828:Plant Cells / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011789:Quantum Theory
chemical_list: D002247:Carbon Isotopes; D052578:Ionic Liquids; D011134:Polysaccharides; C007916:hemicellulose; D008031:Lignin