title: The ankyrin-repeat transmembrane protein BDA1 functions downstream of the receptor-like protein SNC2 to regulate plant immunity.
abstract: Plants utilize a large number of immune receptors to recognize pathogens and activate defense responses. A small number of these receptors belong to the receptor-like protein family. Previously, we showed that a gain-of-function mutation in the receptor-like protein SNC2 (for Suppressor of NPR1, Constitutive2) leads to constitutive activation of defense responses in snc2-1D mutant plants. To identify defense signaling components downstream of SNC2, we carried out a suppressor screen in the snc2-1D mutant background of Arabidopsis (Arabidopsis thaliana). Map-based cloning of one of the suppressor genes, BDA1 (for bian da; "becoming big" in Chinese), showed that it encodes a protein with amino-terminal ankyrin repeats and carboxyl-terminal transmembrane domains. Loss-of-function mutations in BDA1 suppress the dwarf morphology and constitutive defense responses in snc2-1D npr1-1 (for nonexpressor of pathogenesis-related genes1,1) and also result in enhanced susceptibility to bacterial pathogens. In contrast, a gain-of-function allele of bda1 isolated from a separate genetic screen to search for mutants with enhanced pathogen resistance was found to constitutively activate cell death and defense responses. These data suggest that BDA1 is a critical signaling component that functions downstream of SNC2 to regulate plant immunity.
authors: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China 102206.
authors: Yuanai
authors: Y
authors: Yang
authors: Yaxi
authors: Y
authors: Zhang
authors: Pingtao
authors: P
authors: Ding
authors: Kaeli
authors: K
authors: Johnson
authors: Xin
authors: X
authors: Li
authors: Yuelin
authors: Y
authors: Zhang
mesh_terms: D000483:Alleles; D017089:Ankyrin Repeat; D017360:Arabidopsis / Q000166:cytology / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism*; D001483:Base Sequence; D016923:Cell Death; D003001:Cloning, Molecular; D060467:Disease Resistance / Q000276:immunology; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity / Q000276:immunology*; D044224:Pseudomonas syringae / Q000502:physiology; D051192:Receptors, Pattern Recognition / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins; C105496:NPR1 protein, Arabidopsis; D051192:Receptors, Pattern Recognition; D014157:Transcription Factors; C487183:WRKY70 protein, Arabidopsis