title: [Laboratory-based evaluation of "INOVA/QUANTA Lite" to determine antinuclear antibodies (ANA) and autoantibodies to double-stranded DNA, SS-A and SS-B].
abstract: We evaluated QUANTA Lite reagent series (INOVA Diagnostics, CA, USA) to determine antinuclear antibodies (ANA) and autoantibodies to double-stranded (ds) DNA, SS-A and SS-B, in parallel with MESACUP (Medical & Biological Laboratories, Nagoya). Overall agreements between two reagents for qualitative interpretation ranged from 77.5% (ANA) to 99.0%(anti-SS-B antibodies). When we compared to the results by indirect fluorescent antibody (IFA) test on HEp-2 cells, QUANTA Lite ANA demonstrated better sensitivity and specificity; 92.2% versus 76.5% in sensitivity and 92.1% versus 86.8% in specificity. Also, determining anti-chromatin antibodies and IFA test onto Chrithidia luciliae demonstrated greater interpretive correlation to detect anti-ds DNA by QUANTA Lite than by MESACUP. All the discrepant sera to which QUANTA Lite SS-A gave positive interpretations were confirmed to contain the antibodies specific to SS-A 52kDa antigen, which is supplemented to QUANTA Lite capture-probes. With these results, we can conclude that QUANTA Lite has superiorities over MESACUP; (1) to detect a variety of autoantibodies consisting of ANA, (2) to have a better correlation with confirmatory tests to detect anti-ds DNA antibodies, (3)to detect additional autoantibodies specific to SS-A 52kDa antigen, and (4) to have an enough compatibility in determining anti-SS-B antibodies.
authors: Clinical Laboratories, University Hospital of the Ryukyus, Nakagami-gun, Okinawa-pref. 903-0215, Japan. yameg@med.u-ryukyu.ac.jp
authors: Megumi S
authors: MS
authors: Yamauchi
authors: Naohiko
authors: N
authors: Shingaki
authors: Nobuhisa
authors: N
authors: Yamane
mesh_terms: D000974:Antibodies, Antinuclear / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D004247:DNA / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D011933:Reagent Kits, Diagnostic; C035356:SS-A antibodies; C035357:SS-B antibodies; D004247:DNA