title: Disrupted circadian rhythm in rats with nephrectomy-induced chronic kidney disease.
abstract: OBJECTIVE
  Our study investigated the role of circadian rhythm in the pathogenesis of sleep disturbance in patients with chronic kidney disease (CKD) based on an animal model.
  
  
  METHODS
  Sixteen Sprague-Dawley (SD) rats (eight from 5/6 nephrectomized CKD group and eight from control group) were used for electroencephalography (EEG) and electromyography (EMG) recording. Eight rats (four from CKD and four from control group) were sacrificed at six Zeitgeber time (ZT) points and determined the mRNA expression of clock genes, rPer1, rPer2 and rBMAL1b in the hypothalamus.
  
  
  RESULTS
  Our results demonstrated that both slow wave sleep (SWS) and rapid eye movement (REM) sleep were significantly increased in the ZT22-24 Zeitgeber time point of the dark period in the CKD rats when compared with those sleep architectures obtained from the control rats. The CKD-induced sleep disruptions were associated with significant upregulations of rPer1 (in ZT2, ZT6 and ZT14) and rPer2 mRNA expression (in ZT2 and ZT14) in the hypothalamus.
  
  
  CONCLUSIONS
  Our study elucidated that the increases of SWS and REM sleep during ZT22-24 of the dark period in the CKD rats might be due to the enhancement of rPer1 and rPer2 clock genes in the hypothalamus, suggesting that disrupted circadian rhythm plays a role in the pathogenesis of sleep disturbance in patients with CKD.
authors: Department of Neurology, Kaohsiung Medical University Hospital, No. 100, Tzyou 1st Road, Kaohsiung City 807, Taiwan.
authors: Chung-Yao
authors: CY
authors: Hsu
authors: Fang-Chia
authors: FC
authors: Chang
authors: Hwee-Yeong
authors: HY
authors: Ng
authors: Chien-Chun
authors: CC
authors: Kuo
authors: Yueh-Ting
authors: YT
authors: Lee
authors: Chin-Yu
authors: CY
authors: Lu
authors: Chien-Te
authors: CT
authors: Lee
mesh_terms: D000818:Animals; D002940:Circadian Rhythm; D004569:Electroencephalography / Q000379:methods; D004576:Electromyography / Q000379:methods; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000503:physiopathology*; D008297:Male; D009392:Nephrectomy / Q000379:methods*; D056950:Period Circadian Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep; D012895:Sleep, REM; D013997:Time Factors
chemical_list: C539024:Per1 protein, rat; C513311:Per2 protein, rat; D056950:Period Circadian Proteins; D012333:RNA, Messenger