title: Differential developmental deficits in retinal function in the absence of either protein tyrosine sulfotransferase-1 or -2.
abstract: To investigate the role(s) of protein-tyrosine sulfation in the retina and to determine the differential role(s) of tyrosylprotein sulfotransferases (TPST) 1 and 2 in vision, retinal function and structure were examined in mice lacking TPST-1 or TPST-2. Despite the normal histologic retinal appearance in both Tpst1(-/-) and Tpst2(-/-) mice, retinal function was compromised during early development. However, Tpst1(-/-) retinas became electrophysiologically normal by postnatal day 90 while Tpst2(-/-) mice did not functionally normalize with age. Ultrastructurally, the absence of TPST-1 or TPST-2 caused minor reductions in neuronal plexus. These results demonstrate the functional importance of protein-tyrosine sulfation for proper development of the retina and suggest that the different phenotypes resulting from elimination of either TPST-1 or -2 may reflect differential expression patterns or levels of the enzymes. Furthermore, single knock-out mice of either TPST-1 or -2 did not phenocopy mice with double-knockout of both TPSTs, suggesting that the functions of the TPSTs are at least partially redundant, which points to the functional importance of these enzymes in the retina.
authors: Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States of America.
authors: David M
authors: DM
authors: Sherry
authors: Yogita
authors: Y
authors: Kanan
authors: Robert
authors: R
authors: Hamilton
authors: Adam
authors: A
authors: Hoffhines
authors: Kelsey L
authors: KL
authors: Arbogast
authors: Steven J
authors: SJ
authors: Fliesler
authors: Muna I
authors: MI
authors: Naash
authors: Kevin L
authors: KL
authors: Moore
authors: Muayyad R
authors: MR
authors: Al-Ubaidi
mesh_terms: D000818:Animals; D004596:Electroretinography; D015151:Immunoblotting; D051379:Mice; D018345:Mice, Knockout; D008854:Microscopy, Electron; D012160:Retina / Q000201:enzymology* / Q000254:growth & development* / Q000502:physiology; D015238:Sulfotransferases / Q000235:genetics / Q000378:metabolism*
chemical_list: D015238:Sulfotransferases; C529648:TPST2 protein, mouse; C039079:protein-tyrosine sulfotransferase