title: [Optimization  of magnetic activated cell sorting  and the biological characteristics of isolated cells].
abstract: OBJECTIVE
  To optimize  the   traditional magnetic activated cell sorting (MACS) so as to  enhance the purification and keep the  viability of cells after separation.
  
  
  METHODS
  Take    stem cell antigen-1  labeled  hemopoietic stem/progenitor cells (Sca-1(+);HSC/HPC)  for example. The traditional MACS and the optimized MACS were applied to obtain the Sca-1(+); HSC/HPC from mouse bone marrow respectively. The purifications of Sca-1(+); cells from two groups were tested by flow cytometry;  The survival rates of Sca-1(+); cells from two groups were detected by trypan blue dye;  The proliferation of Sca-1(+); cells was detected by cell counting kit-8(CCK-8);  the differentiation capacity of Sca-1(+); cells was  measured by CFU-Mix.
  
  
  RESULTS
  The purification of Sca-1(+); cells was up to 93%  by the optimized MACS compared to 87%  by the traditional one;  the recovery rate of positive cells was 73%  by  the optimized MACS compared to 62.3%  by the traditional one; there was no  statistical difference in the viability and proliferation   of Sca-1(+); cells  and the capacity of producing CFU-Mix  between two groups.
  
  
  CONCLUSIONS
  The optimized MACS can significantly enhance the purification and recovery rate of Sca-1(+); cells without influence of cell viability and biological features,  which can be considered to apply in other  MACS.
authors: Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, China. 83612914@qq.com
authors: Shan
authors: S
authors: Geng
authors: Chen
authors: C
authors: Zhang
authors: Jun
authors: J
authors: Liu
authors: Dian-feng
authors: DF
authors: Liu
authors: Yue
authors: Y
authors: Zhou
authors: Ya-ping
authors: YP
authors: Wang
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism*; D003114:Colony-Forming Units Assay; D005260:Female; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D018189:Immunomagnetic Separation / Q000379:methods*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins