title: Formation and preclinical evaluation of a new alloplastic injectable bone substitute material.
abstract: Alloplastic bone substitute materials are raising some more interest as an alternative for autologic transplants and xenogenic materials especially in oral surgery over the last few years. These non-immunogenic and completely resorbable biomaterials are the basis for complete and predictable guided bone regeneration. In the majority of cases, such a material is chosen because of its convenient application by surgeons. The main objective of our project was to design and fabricate an osteoconductive, injectable and readily tolerable by human tissues biomaterial for guided bone regeneration. For this purpose, a self-setting composite consisting of chitosan/tricalcium phosphate microparticles and sodium alginate was made. The material obtained was characterized by microsphere and agglomerate morphology and microstructure. Its features relating to setting time and mechanical properties were precisely investigated. Our material was also evaluated according to PN-EN ISO 10993 Biological evaluation of medical devices, i.e., the in vitro tests for genotoxicity and cytotoxicity were conduced. Then, the following examinations were performed: subchronic systemic toxicity, skin sensitization, irritation and delayed-type hypersensitivity and local effects after implantation. The material tested showed a high degree of cytocompatibility, fulfilled the requirements of International Standards and seemed to be a "user friendly" material for oral surgeons.
authors: Medical Devices Department, National Medicines Institute, Warsaw, Poland. bojar@il.waw.pl
authors: Witold
authors: W
authors: Bojar
authors: Martyna
authors: M
authors: Kucharska
authors: Grzegorz
authors: G
authors: Bubak
authors: Tomasz
authors: T
authors: Ciach
authors: Łukasz
authors: Ł
authors: Koperski
authors: Zenon
authors: Z
authors: Jastrzębski
authors: Beata M
authors: BM
authors: Gruber
authors: Jolanta
authors: J
authors: Krzysztoń-Russjan
authors: Jadwiga
authors: J
authors: Marczewska
authors: Elżbieta L
authors: EL
authors: Anuszewska
authors: Ewa
authors: E
authors: Drozd
authors: Tomasz
authors: T
authors: Brynk
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology*; D018786:Bone Substitutes / Q000138:chemical synthesis* / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology; D002122:Calcium Chloride / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology; D048271:Chitosan / Q000494:pharmacology; D006801:Humans; D007267:Injections; D008297:Male; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D019919:Prosthesis Implantation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D048271:Chitosan; C018392:tricalcium phosphate; D002122:Calcium Chloride