title: An innovative bi-layered wound dressing made of silk and gelatin for accelerated wound healing.
abstract: In this study, the novel silk fibroin-based bi-layered wound dressing was developed. Wax-coated silk fibroin woven fabric was introduced as a non-adhesive layer while the sponge made of sericin and glutaraldehyde-crosslinked silk fibroin/gelatin was fabricated as a bioactive layer. Wax-coated silk fibroin fabrics showed improved mechanical properties compared with the non-coated fabrics, but less adhesive than the commercial wound dressing mesh. This confirmed by results of peel test on both the partial- and full-thickness wounds. The sericin-silk fibroin/gelatin spongy bioactive layers showed homogeneous porous structure and controllable biodegradation depending on the degree of crosslinking. The bi-layered wound dressings supported the attachment and proliferation of L929 mouse fibroblasts, particularly for the silk fibroin/gelatin ratio of 20/80 and 0.02% GA crosslinked. Furthermore, we proved that the bi-layered wound dressings promoted wound healing in full-thickness wounds, comparing with the clinically used wound dressing. The wounds treated with the bi-layered wound dressings showed the greater extent of wound size reduction, epithelialization, and collagen formation. The superior properties of the silk fibroin-based bi-layered wound dressings compared with those of the clinically used wound dressings were less adhesive and had improved biological functions to promote cell activities and wound healing. This novel bi-layered wound dressing should be a good candidate for the healing of full-thickness wounds.
authors: Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, PhayaThai Road, Phatumwan, Bangkok 10330, Thailand.
authors: Sorada
authors: S
authors: Kanokpanont
authors: Siriporn
authors: S
authors: Damrongsakkul
authors: Juthamas
authors: J
authors: Ratanavaraporn
authors: Pornanong
authors: P
authors: Aramwit
mesh_terms: D000818:Animals; D001458:Bandages; D002460:Cell Line; D049109:Cell Proliferation; D005349:Fibroins / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D066298:In Vitro Techniques; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D047030:Sericins / Q000737:chemistry*; D013552:Swine; D014885:Waxes / Q000737:chemistry; D014945:Wound Healing
chemical_list: D047030:Sericins; D014885:Waxes; D005780:Gelatin; D005349:Fibroins; C026344:carnauba wax