title: Bioconjugated gold nanorods to enhance the sensitivity of FT-SPR-based biosensors.
abstract: In this paper, we report a feasible solvent-free approach for the synthesis and self-assembling of gold nanorods after bioconjugation to antibodies; scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images revealed a remarkable shape and size narrow distribution, as well as a tendency to form linear assemblies on a clean gold surface, upon interaction with antibodies. These bioconjugated gold nanorods were in turns tested in a model Fourier Transform Surface Plasmon Resonance (FT-SPR)-based immunosensor, leading to an improvement of the detection sensitivity by a factor of 8. The results highlight the simplicity of the synthesis protocol of gold nanorods and the interest of using them as labels to enhance the sensitivity of SPR-based sensors.
authors: Laboratoire de Réactivité de Surface, UMR CNRS 7197, Université Pierre et Marie Curie-Paris VI, Paris, France. jolanda.spadavecchia@upmc.fr
authors: Jolanda
authors: J
authors: Spadavecchia
authors: Sandra
authors: S
authors: Casale
authors: Souhir
authors: S
authors: Boujday
authors: Claire-Marie
authors: CM
authors: Pradier
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry*; D015374:Biosensing Techniques; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D018796:Immunoconjugates / Q000737:chemistry*; D007074:Immunoglobulin G / Q000032:analysis*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D043942:Nanotubes / Q000737:chemistry*; D010316:Particle Size; D011817:Rabbits; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance
chemical_list: D000888:Antibodies, Anti-Idiotypic; D002593:Cetrimonium Compounds; D018796:Immunoconjugates; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D006046:Gold; D000077286:Cetrimonium