title: [Effect of ultraviolet radiation on ALDH1 expression in human lens epithelial cells].
abstract: OBJECTIVE
  To determine the apoptosis-inducing effect of ultraviolet light (UV) on human lens epithelial cell (HLEC) and to explore the involvement of changes in ALDH1 folowing UV radiation.
  
  
  METHODS
  HLEC was exposed to the same UV light source and was subsequently divided into 6 groups according to UV radiation time of 0 (control group), 5, 10, 15, and 30 min. Apoptosis was detected by AO/EB staining. Changes of ALDH1 in HLEC were detected by immunohistochemical staining and Western blot.
  
  
  RESULTS
  The intensity of immunohistochemical staining and the rate of positive cells decreased with increase of UV time (P<0.05). The rate of positive ALDH1 cells was negatively correlated with the rate of apoptosis (r= -0.92, P<0.05). Western blot showed the integrated absorbance values significantly decreased with the increase of UV time (P<0.05).
  
  
  CONCLUSIONS
  ALDH1 in HLEC decreases with an increase of UV exposure, which may be related to UV induced apoptosis of HLEC.
authors: Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors: Jingming
authors: J
authors: Shi
authors: Songbai
authors: S
authors: Jia
authors: Xuan
authors: X
authors: Chen
authors: Luosheng
authors: L
authors: Tang
mesh_terms: D000080924:Aldehyde Dehydrogenase 1 Family; D017209:Apoptosis / Q000528:radiation effects*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007908:Lens, Crystalline / Q000166:cytology*; D050697:Retinal Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D007527:Isoenzymes; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase