title: Occurrence of the aacA4 gene among multidrug resistant strains of Pseudomonas aeruginosa isolated from bronchial secretions obtained from the Intensive Therapy Unit at University Hospital in Bialystok, Poland.
abstract: The aim of this study was to investigate the prevalence of the aacA4 gene in a population of multidrug resistant strains of P. aeruginosa isolated from bronchial secretions obtained from the Intensive Therapy Unit (ITU). Twelve MDR isolates were tested for antibiotic susceptibility and the presence of the aacA4 gene. In this study, 58.3% of the strains contained (6')-Ib' aminoglycoside acetyltransferase gene. All of the studied strains (aacA4-positive and aacA4-negative) were susceptible only to colistine (100%). Among other antibiotics, the lowest resistance rates were those shown against ceftazidime (14.3% to 20%) and imipenem (28.6% to 40%). Our studies frequently revealed the presence of the aacA4 gene as a factor responsible for resistance; it is probable that other mechanisms of resistance to aminoglycoside antibiotics also occurred.
authors: Department of Microbiological Diagnostics and Infectious Immunology, Medical University of Bialystok, Poland. sachpt@umb.edu.pl
authors: Paweł
authors: P
authors: Sacha
authors: Jadwiga
authors: J
authors: Jaworowska
authors: Dominika
authors: D
authors: Ojdana
authors: Piotr
authors: P
authors: Wieczorek
authors: Sławomir
authors: S
authors: Czaban
authors: Elżbieta
authors: E
authors: Tryniszewska
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D001980:Bronchi / Q000378:metabolism* / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial / Q000235:genetics*; D006785:Hospitals, University / Q000706:statistics & numerical data*; D006801:Humans; D007362:Intensive Care Units / Q000706:statistics & numerical data*; D008826:Microbial Sensitivity Tests; D011044:Poland / Q000453:epidemiology; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D000617:Aminoglycosides