title: Protective effect of caffeic acid on paclitaxel induced anti-proliferation and apoptosis of lung cancer cells involves NF-κB pathway.
abstract: Caffeic acid (CA), a natural phenolic compound, is abundant in medicinal plants. CA possesses multiple biological effects such as anti-bacterial and anti-cancer growth. CA was also reported to induce fore stomach and kidney tumors in a mouse model. Here we used two human lung cancer cell lines, A549 and H1299, to clarify the role of CA in cancer cell proliferation. The growth assay showed that CA moderately promoted the proliferation of the lung cancer cells. Furthermore, pre-treatment of CA rescues the proliferation inhibition induced by a sub-IC(50) dose of paclitaxel (PTX), an anticancer drug. Western blot showed that CA up-regulated the pro-survival proteins survivin and Bcl-2, the down-stream targets of NF-κB. This is consistent with the observation that CA induced nuclear translocation of NF-κB p65. Our study suggested that the pro-survival effect of CA on PTX-treated lung cancer cells is mediated through a NF-κB signaling pathway. This may provide mechanistic insights into the chemoresistance of cancer calls.
authors: Department of Life Science and Institute of Zoology, National Taiwan University, Taipei 106, Taiwan.
authors: Chien-Liang
authors: CL
authors: Lin
authors: Department of Life Science and Institute of Zoology, National Taiwan University, Taipei 106, Taiwan.
authors: Ruei-Feng
authors: RF
authors: Chen
authors: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Jeff Yi-Fu
authors: JY
authors: Chen
authors: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Ying-Chieh
authors: YC
authors: Chu
authors: Department of Fragrance and Cosmetics Science, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Hui-Min
authors: HM
authors: Wang
authors: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Han-Lin
authors: 0000-0002-5162-9752
authors: HL
authors: Chou
authors: Graduate Institute of Medical Genetics, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Wei-Chiao
authors: WC
authors: Chang
authors: Chest Surgery, Chi-Mei Foundation Medical Center, Yung Kang City, Tainan 901, Taiwan.
authors: Yao
authors: Y
authors: Fong
authors: Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.|Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Wen-Tsan
authors: WT
authors: Chang
authors: Department of Biological Sciences, National Sun Yat-Sen University, 70 Lien Hai Road, Kaohsiung 804, Taiwan.
authors: Chang-Yi
authors: CY
authors: Wu
authors: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Chien-Chih
authors: 0000-0001-7307-2468
authors: CC
authors: Chiu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002109:Caffeic Acids / Q000494:pharmacology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D000077022:Survivin; D015854:Up-Regulation
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C107489:BIRC5 protein, human; D002109:Caffeic Acids; D051034:Inhibitor of Apoptosis Proteins; D016328:NF-kappa B; D000077022:Survivin; D017239:Paclitaxel; C040048:caffeic acid