title: Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge.
abstract: Orally delivered DNA vaccines against duck enteritis virus (DEV) were developed using live attenuated Salmonella typhimurium (SL7207) as a carrier and Escherichia coli heat labile enterotoxin B subunit (LTB) as a mucosal adjuvant. DNA vaccine plasmids pVAX-UL24 and pVAX-LTB-UL24 were constructed and transformed into attenuated Salmonella typhimurium SL7207 resulting SL7207 (pVAX-UL24) and SL7207 (pVAX-LTB-UL24) respectively. After ducklings were orally inoculated with SL7207 (pVAX-UL24) or SL7207 (pVAX-LTB-UL24), the anti-DEV mucosal and systemic immune responses were recorded. To identify the optimum dose that confers maximum protection, we used different doses of the candidate vaccine SL7207 (pVAX-LTB-UL24) during oral immunization. The strongest mucosal and systemic immune responses developed in the SL7207 (pVAX-LTB-UL24) (1011 CFU) immunized group. Accordingly, oral immunization of ducklings with SL7207 (pVAX-LTB-UL24) showed superior efficacy of protection (60-80%) against a lethal DEV challenge (1000 LD50), compared with the limited survival rate (40%) of ducklings immunized with SL7207 (pVAX-UL24). Our study suggests that the SL7207 (pVAX-LTB-UL24) can be a candidate DEV vaccine.
authors: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, People's Republic of China. cqrc_jry@163.com.
authors: Xia
authors: X
authors: Yu
authors: Renyong
authors: R
authors: Jia
authors: Juan
authors: J
authors: Huang
authors: Bin
authors: B
authors: Shu
authors: Dekang
authors: D
authors: Zhu
authors: Qing
authors: Q
authors: Liu
authors: Xinghong
authors: X
authors: Gao
authors: Meng
authors: M
authors: Lin
authors: Zhongqiong
authors: Z
authors: Yin
authors: Mingshu
authors: M
authors: Wang
authors: Shun
authors: S
authors: Chen
authors: Yin
authors: Y
authors: Wang
authors: Xiaoyue
authors: X
authors: Chen
authors: Anchun
authors: A
authors: Cheng
mesh_terms: D000276:Adjuvants, Immunologic / Q000235:genetics; D000284:Administration, Oral; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D004372:Ducks; D004768:Enterotoxins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D018928:Immunity, Mucosal; D025922:Mardivirus / Q000276:immunology*; D008380:Marek Disease / Q000276:immunology* / Q000821:virology; D010957:Plasmids / Q000235:genetics; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology; D011897:Random Allocation; D012486:Salmonella typhimurium / Q000276:immunology; D014613:Vaccines, Attenuated / Q000235:genetics / Q000378:metabolism; D019444:Vaccines, DNA / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines; C027538:heat-labile enterotoxin, E coli