title: High-fat diet causes an imbalance in the colonic serotonergic system promoting adipose tissue enlargement and dysplasia in rats.
abstract: A high-fat (HF) diet, the serotonergic system and stromal elements have all been implicated in colon carcinogenesis. We investigated whether the colonic serotonergic system could play a main role in the development of colonic dysplasia and stromal reactivity in carcinogen-treated rats under HF diet. For this, dimethylhydrazine-treated rats were fed with standard diet and a HF diet. Fat distribution was quantified by computerized tomography exam, serotonergic activity was analyzed by high-performance liquid chromatography, gene expression, and immunohistochemistry, which along with histopathological technique enabled us to enumerate dysplasia, microvessels density, cell proliferation and COX-2 expression. We found that the HF diet induced an increase in the amount of visceral adipose tissue, even without expressive changes in the average body weight. This was correlated with a loss of serotonergic balance in colon tissue. Moreover, the HF diet promoted dysplasia and microvessel density in association with increased proliferation and COX-2 expression within pericryptal colonic stroma. Our current findings suggest that a HF diet promotes the enlargement of adipose tissue via loss of control in colon serotonergic activity, which enhances colonic dysplasia by supporting microvessel development.
authors: Department of Pathology, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. vinicius.cardoso@uni-wuerzburg.de
authors: Vinicius
authors: V
authors: Kannen
authors: Dalila L
authors: DL
authors: Zanette
authors: Cleverson R
authors: CR
authors: Fernandes
authors: Frederico R
authors: FR
authors: Ferreira
authors: Tassiana
authors: T
authors: Marini
authors: Milene C
authors: MC
authors: Carvalho
authors: Marcus L
authors: ML
authors: Brandão
authors: Jorge
authors: J
authors: Elias Junior
authors: Fernando M
authors: FM
authors: Mauad
authors: Wilson A
authors: WA
authors: Silva
authors: Helga
authors: H
authors: Stopper
authors: Sérgio B
authors: SB
authors: Garcia
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000008:administration & dosage; D005260:Female; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007150:Immunohistochemistry; D050152:Intra-Abdominal Fat / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D012701:Serotonin / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D012313:RNA; D051546:Cyclooxygenase 2