title: Expression of hypoxia-related glucose transporters GLUT1 and GLUT3 in             benign, malignant and non-neoplastic thyroid lesions.
abstract: The enhancement of glucose metabolism in neoplastic cells is mediated by             the overexpression of key glycolytic enzymes and glucose transporters (GLUTs).             In particular, an increased expression of hypoxia-related GLUT1 and GLUT3 has             been found in a variety of malignancies. The aim of this study was to examine             the expression levels of GLUT1 and GLUT3 in benign and malignant thyroid tumors,             as well as in non-neoplastic lesions. Analysis of the mRNA expression levels of             solute carrier family 2, member 1 (SLC2A1) and solute carrier family 2, member 3             (SLC2A3) (genes coding GLUT1 and GLUT3, respectively) was performed by the real-time             PCR method with fluorescent probes. GLUT1 and GLUT3 protein expression levels             were determined in thyroid specimens by immunodetection after separation of proteins             on 10% polyacrylamide slab gels and electrotransfer onto Immobilon-P membranes.             The majority of papillary carcinoma samples showed a higher expression of GLUT1             and GLUT3 in comparison with follicular carcinoma cases and non-neoplastic thyroid             lesions. A tendency towards an increased expression of GLUT1 and GLUT3 was observed             in papillary carcinoma cases with more advanced disease stages. Moreover, a significant             correlation was noted between the hypoxia-related GLUT1 and GLUT3 expression determined             by both methods. In conclusion, our findings suggest that GLUT1 and GLUT3 play             an important role in the pathology of thyroid glands.
authors: Department of Cytobiochemistry, University of Łódź, 90-236 Łódź,                     Poland.
authors: Paweł
authors: P
authors: Jóźwiak
authors: Anna
authors: A
authors: Krześlak
authors: Lech
authors: L
authors: Pomorski
authors: Anna
authors: A
authors: Lipińska
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002291:Carcinoma, Papillary / Q000378:metabolism / Q000473:pathology; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism*; D051274:Glucose Transporter Type 3 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D000860:Hypoxia; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D013961:Thyroid Gland / Q000378:metabolism* / Q000473:pathology; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D005456:Fluorescent Dyes; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3