title: Neuroprotective effects and magnetic resonance imaging of mesenchymal stem cells labeled with SPION in a rat model of Huntington's disease.
abstract: Bone marrow mesenchymal stem cells (MSC) have been tested and proven effective in some neurodegenerative diseases, but their tracking after transplantation may be challenging. Our group has previously demonstrated the feasibility and biosafety of rat MSC labeling with iron oxide superparamagnetic nanoparticles (SPION). In this study, we investigated the therapeutic potential of SPION-labeled MSC in a rat model of Huntington's disease, a genetic degenerative disease with characteristic deletion of striatal GABAergic neurons. MSC labeled with SPION were injected into the striatum 1h after quinolinic acid injection. FJ-C analysis demonstrated that MSC transplantation significantly decreased the number of degenerating neurons in the damaged striatum 7 days after lesion. In this period, MSC transplantation enhanced the striatal expression of FGF-2 but did not affect subventricular zone proliferation, as demonstrated by Ki67 proliferation assay. In addition, MSC transplantation significantly reduced the ventriculomegaly in the lesioned brain. MRI and histological techniques detected the presence of the SPION-labeled cells at the lesion site. SPION-labeled MSC produced magnetic resonance imaging (MRI) signals that were visible for at least 60 days after transplantation. Our data highlight the potential of adult MSC to reduce brain damage under neurodegenerative diseases and indicate the use of nanoparticles in cell tracking, supporting their potential as valuable tools for cell therapy.
authors: Centro de Ciências da Saúde, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
authors: Louise
authors: L
authors: Moraes
authors: Andreia
authors: A
authors: Vasconcelos-dos-Santos
authors: Fernando Cleber
authors: FC
authors: Santana
authors: Mariana Araya
authors: MA
authors: Godoy
authors: Paulo Henrique
authors: PH
authors: Rosado-de-Castro
authors: Jasmin
authors: Ricardo Luiz
authors: RL
authors: Azevedo-Pereira
authors: Wagner Monteiro
authors: WM
authors: Cintra
authors: Emerson Leandro
authors: EL
authors: Gasparetto
authors: Marcelo Felippe
authors: MF
authors: Santiago
authors: Rosalia
authors: R
authors: Mendez-Otero
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003911:Dextrans / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005295:Ferrocyanides / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006816:Huntington Disease / Q000473:pathology / Q000628:therapy*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000627:therapeutic use*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D053758:Nanoparticles / Q000627:therapeutic use*; D017072:Neostriatum / Q000187:drug effects / Q000473:pathology; D009410:Nerve Degeneration / Q000473:pathology / Q000628:therapy; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D003911:Dextrans; D005295:Ferrocyanides; D058185:Magnetite Nanoparticles; D018696:Neuroprotective Agents; D016222:Fibroblast Growth Factor 2; C065507:ferumoxides; C000170:ferric ferrocyanide