title: [Construction of recombinant baculovirus co-expressing M1 and HA of influenza A virus].
abstract: The M1 and HA genes of H1N1 influenza virus were amplified and then cloned into the pFastBac dual donor plasmid. The recombinant pFastBac Dual-M1-HA was identified by restriction enzyme digestion. After the pFastBacdual-M1-HA was transformed into the baculovirus shuttle plasmid (bacmid) in DH10Bac competent cells, the colonies were identified by antibiotics and blue-white selection. The rBac-mid-M1-HA was verified by PCR and transfected into S f9 cells to produce recombinant baculovirus (rBac-M1-HA). Gene insertion of rBac-M1-HA was verified and the expression of M1 and HA genes was analyzed by IFA and Western-blot, demonstrating M1 and HA were co-expressed successfully. This study provides the foundation for researching the formation mechanism of influenza VLP and developing new influenza vaccines.
authors: College of Life Science and Science, Sichuan Agricultural University, Yaan 625014, China. mengzhonren@163.com
authors: Peng-Wei
authors: PW
authors: Xu
authors: Jian-Qiang
authors: JQ
authors: Guo
authors: Li-Hong
authors: LH
authors: Yao
authors: Ai-Jun
authors: AJ
authors: Chen
authors: Xiao-Yu
authors: XY
authors: Liu
authors: Xian-Yin
authors: XY
authors: Zeng
authors: Zhi-Qing
authors: ZQ
authors: Zhang
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000276:immunology; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000276:immunology; D018411:Spodoptera; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus; C488936:M1 protein, Influenza A virus; D014763:Viral Matrix Proteins