title: Confirming the functional importance of a protein-DNA interaction.
abstract: Identifying DNA-binding proteins that interact with a control region of interest has become quite straightforward. However, the functional relevance of a given protein-DNA interaction is difficult to establish. The hypothesis that an interaction is relevant can be tested by several different experiments, 12 of which are outlined in this article. It must be remembered that none of these experiments by itself is conclusive. The information gained from each approach is described and explanations are given for why each yields useful but inconclusive results. The approaches vary widely with respect to the amount of effort required and the quality of information obtained.
authors: Michael F
authors: MF
authors: Carey
authors: Craig L
authors: CL
authors: Peterson
authors: Stephen T
authors: ST
authors: Smale
mesh_terms: D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods*; D011485:Protein Binding
chemical_list: D004268:DNA-Binding Proteins; D004247:DNA