title: Merging of confocal and caging technologies: selective three-color communication with profluorescent reporters.
abstract: Falling apart, on cue: Signaling pathways often display a profound spatiotemporal component that is best studied using light-activatable reagents. Three separate photolabile moieties that can be distinguished based upon their response to three distinct wavelengths (360, 440, and 560 nm) have been synthesized and evaluated. This tri-color system is also applied to imaging in microwells and HeLa cells (see picture).
authors: Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA.
authors: Melanie A
authors: MA
authors: Priestman
authors: Thomas A
authors: TA
authors: Shell
authors: Liang
authors: L
authors: Sun
authors: Hsien-Ming
authors: HM
authors: Lee
authors: David S
authors: DS
authors: Lawrence
mesh_terms: D002614:Chelating Agents / Q000737:chemistry*; D003116:Color; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D015394:Molecular Structure; D010782:Photolysis
chemical_list: D002614:Chelating Agents