title: Use of MDLC-DIGE and LC-MS/MS to identify serum biomarkers for complete remission in patients with acute myeloid leukemia.
abstract: The response criteria for complete remission (CR) in acute myeloid leukemia (AML) are currently based on morphology and blood cell counts. However, these criteria are insufficient to establish a diagnosis in cases with poor quality bone marrow (BM) samples demonstrating a loss of cellular morphology. We investigated whether the sera of patients contained biomarkers that indicate disease response status. First, we performed multidimensional liquid chromatography-differential gel electrophoresis (MDLC-DIGE) to generate protein profiles of two pooled, paired serum samples from patients who had achieved CR; one collected at diagnosis (PreCR) and the other collected after chemotherapy (CR). Then, with the biomarker candidates found, ELISA was carried out for individual PreCR and CR samples, and for other verification sets including nonremission (NR) patients and normal samples. We selected two proteins, complement factor H (CFH) and apolipoprotein H (ApoH), with dye (Cy) ratios showing greater than 2.0-fold differences between the pooled samples. ELISA showed that CFH and ApoH are useful for distinguishing between the recovered (CR and normal) and nonrecovered (PreCR, PreNR, and NR) states in AML (p <0.001). We successfully applied a protein profiling technology of MDLC-DIGE and LC-MS/MS to discover two biomarkers for CR which needs further validation for a clinical setting.
authors: Department of Anatomy, Chonnam National University Medical School, Gwangju, Korea. seunglee@chonnam.ac.kr
authors: Seung-Won
authors: SW
authors: Lee
authors: In Jae
authors: IJ
authors: Kim
authors: Bo Yoon
authors: BY
authors: Jeong
authors: Mun-Ho
authors: MH
authors: Choi
authors: Jin Young
authors: JY
authors: Kim
authors: Kyung-Hoon
authors: KH
authors: Kwon
authors: Jae Won
authors: JW
authors: Lee
authors: Ami
authors: A
authors: Yu
authors: Myung-Geun
authors: MG
authors: Shin
mesh_terms: D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D017242:Complement Factor H / Q000032:analysis; D018572:Disease-Free Survival; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood* / Q000188:drug therapy; D008297:Male; D008875:Middle Aged; D053719:Tandem Mass Spectrometry / Q000379:methods*; D053482:beta 2-Glycoprotein I / Q000097:blood
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D053482:beta 2-Glycoprotein I; D017242:Complement Factor H