title: Phytochemical composition and toxicity of an antioxidant extract from Pimpinella brachycarpa (Kom.) Nakai.
abstract: Pimpinella brachycarpa (Kom.) Nakai (PB) is one of the most favored edible greens grown in Asian regions. In our previously study, we found PB extract had antioxidant effects in vitro. In the present study, an EtOAc soluble extract (PBet) was isolated from PB. Then the antioxidant properties at cellular level, phytochemical composition and toxicity of PBet were examined. The results indicated that PBet (0.5-2mg/mL) could protect Bel-7404 cells from H(2)O(2) induced cell damage through scavenging of intracellular ROS. Moreover, myristic acid, 24ζ-methyl-5α-lanosta-25-one, β-sitosterol, pregnenolone and β-daucosterol were firstly isolated from PB. In addition, PBet (0.75g/kg BW, ig) had no acute toxicity and it (0.03-0.12g/kg BW, ig, 7 d) could not influence the rate of bone marrow polychromatic erythrocytes micronucleus and chromosome aberration in KM mice. All above findings suggested that PBet could be considered as a safe functional food with antioxidant activities.
authors: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, Jilin 130062, People's Republic of China; Institute of Zoonoses, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, People's Republic of China.
authors: Jing
authors: J
authors: Lu
authors: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, Jilin 130062, People's Republic of China.
authors: Wenhui
authors: W
authors: Qian
authors: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, Jilin 130062, People's Republic of China.
authors: Linli
authors: L
authors: Xu
authors: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, Jilin 130062, People's Republic of China.
authors: Guoren
authors: G
authors: Huang
authors: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, Jilin 130062, People's Republic of China.
authors: Wen
authors: W
authors: Cong
authors: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, Jilin 130062, People's Republic of China.
authors: Zhenning
authors: Z
authors: Wang
authors: Institute of Zoonoses, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, People's Republic of China.
authors: Xuming
authors: X
authors: Deng
authors: Institute of Zoonoses, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, People's Republic of China.
authors: Dacheng
authors: D
authors: Wang
authors: Department of Food Quality and Safety, College of Light Industry Economics and Management, Jilin University, Changchun, Jilin 130062, People's Republic of China; Institute of Zoonoses, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, People's Republic of China. Electronic address: gshuang1973@126.com.
authors: Shuang
authors: S
authors: Guan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000633:toxicity*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D051379:Mice; D009152:Mutagenicity Tests; D028042:Pimpinella; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012855:Sitosterols / Q000032:analysis; D023382:Toxicity Tests, Acute
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D012855:Sitosterols; C025473:gamma-sitosterol