title: Titanium-containing magnetic mesoporous silica spheres: effective enrichment of peptides and simultaneous separation of nonphosphopeptides and phosphopeptides.
abstract: Protein phosphorylation is a common posttranslational modification, and involved in many cellular processes. Like endogenous peptides, endogenous phosphopeptides contain many biomarkers of preclinical screening and disease diagnosis. In this work, titanium-containing magnetic mesoporous silica spheres were synthesized and applied for effective enrichment of peptides from both tryptic digests of standard proteins and human serum. Besides, the enriched peptides can be further separated into nonphosphopeptides and phosphopeptides by a simple elution. First, titanium-containing magnetic mesoporous silica spheres were synthesized by a sol-gel method and found to have high surface area, narrow pore size distribution, and useful magnetic responsivity. Then, as the prepared material was used for selective capturing of phosphopeptides, it demonstrated to have higher selectivity than commercial titanium dioxide. Moreover, via combination of size-exclusion mechanism, hydrophobic interaction, and affinity chromatography, titanium-containing magnetic mesoporous silica spheres were successfully applied to simultaneously extract and separate nonphosphopeptides and phosphopeptides from standard protein digestion and human serum.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, P R China.
authors: Xiao-Shui
authors: XS
authors: Li
authors: Xin
authors: X
authors: Su
authors: Gang-tian
authors: GT
authors: Zhu
authors: Yong
authors: Y
authors: Zhao
authors: Bi-Feng
authors: BF
authors: Yuan
authors: Lin
authors: L
authors: Guo
authors: Yu-Qi
authors: YQ
authors: Feng
mesh_terms: D000327:Adsorption; D008280:Magnetics / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D014025:Titanium / Q000737:chemistry
chemical_list: D010455:Peptides; D010748:Phosphopeptides; C009495:titanium dioxide; D012822:Silicon Dioxide; D014025:Titanium