title: Bone morphogenetic protein-derived peptide promotes chondrogenic differentiation of human mesenchymal stem cells.
abstract: Directing chondrogenic differentiation of human mesenchymal stem cells (MSCs) is currently a challenging problem in tissue engineering of cartilage. Short-peptide motifs are promising new tools to aid in controlling chondrogenesis. The aim of this study was to investigate whether a short bone morphogenetic protein-2 (BMP-2)-derived peptide (BMP peptide) stimulates chondrogenesis of human MSCs in the absence of other growth factors. A high-throughput pellet culture system was used to rapidly collect biochemical data such as glycosaminoglycan (GAG), total collagen, and DNA content, as well as alkaline phosphatase (AP) activity. Cells cultured with ≥100 μg/mL of the peptide produced 74% of the GAG content that cells cultured with BMP-2 produced. Comparable levels of GAG production were promoted by the peptide and BMP-2 over 4 weeks of culture. However, histology revealed that the peptide promoted a more homogenous distribution of GAG than BMP-2. The BMP peptide directed human MSCs to increase collagen production after 3 weeks, but at significantly lower levels compared to BMP-2. Treatment with BMP-2 resulted in a large increase in hypertrophic markers such as AP activity and gene expression of type X collagen, whereas treatment with the peptide resulted in little-to-no increase in these markers. These results suggest that the BMP peptide could be an effective new tool for cartilage tissue engineering.
authors: School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA. julieliu@purdue.edu
authors: Julie N
authors: JN
authors: Renner
authors: Yeji
authors: Y
authors: Kim
authors: Julie C
authors: JC
authors: Liu
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics; D000595:Amino Acid Sequence; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects*; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D005109:Extracellular Matrix / Q000187:drug effects; D020869:Gene Expression Profiling; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006801:Humans; D006984:Hypertrophy; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D013268:Stimulation, Chemical
chemical_list: D055396:Bone Morphogenetic Protein 2; D006025:Glycosaminoglycans; D010446:Peptide Fragments; D003094:Collagen; C548267:ALPL protein, human; D000469:Alkaline Phosphatase