title: Microscopy imaging and quantitative phase contrast mapping in turbid microfluidic channels by digital holography.
abstract: We show that sharp imaging and quantitative phase-contrast microcopy is possible in microfluidics in flowing turbid media by digital holography. In fact, in flowing liquids with suspended colloidal particles, clear vision is hindered and cannot be recovered by any other microscopic imaging technique. On the contrary, using digital holography, clear imaging is possible thanks to the Doppler frequency shift experienced by the photons scattered by the flowing colloidal particles, which do not contribute to the interference process, i.e. the recorded hologram. The method is illustrated and imaging results are demonstrated for pure phase objects, i.e. biological cells in microfluidic channels.
authors: CNR-National Institute of Optics, Via Campi Flegrei, 34, I-80078, Pozzuoli (NA), Italy. melania.paturzo@ino.it
authors: Melania
authors: M
authors: Paturzo
authors: Andrea
authors: A
authors: Finizio
authors: Pasquale
authors: P
authors: Memmolo
authors: Roberto
authors: R
authors: Puglisi
authors: Donatella
authors: D
authors: Balduzzi
authors: Andrea
authors: A
authors: Galli
authors: Pietro
authors: P
authors: Ferraro
mesh_terms: D000818:Animals; D002417:Cattle; D003102:Colloids / Q000737:chemistry; D006696:Holography; D007090:Image Interpretation, Computer-Assisted; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D008892:Milk / Q000502:physiology; D017785:Photons; D014867:Water / Q000502:physiology
chemical_list: D003102:Colloids; D014867:Water