title: Resolution of two overlapping neutralizing B cell epitopes within a solvent exposed, immunodominant α-helix in ricin toxin's enzymatic subunit.
abstract: Residues Y₉₁-T₁₁₆ of ricin toxin's enzymatic subunit (RTA) constitute an immunodominant loop-helix-loop motif that is the target of two potent toxin neutralizing monoclonal antibodies (mAbs), PB10 and R70. To define the exact epitope(s) recognized by these mAbs, we affinity enriched from a phage-displayed peptide library 12 mers that bound one or both of these mAbs. We report that PB10 recognizes a distinct but overlapping epitope with R70, in which residues Q₉₈, E₁₀₂, T₁₀₅, and H₁₀₆ are central to mAb recognition.
authors: Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA.
authors: David J
authors: DJ
authors: Vance
authors: Nicholas J
authors: NJ
authors: Mantis
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000378:metabolism; D020134:Catalytic Domain / Q000235:genetics; D018985:Epitopes, B-Lymphocyte / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019151:Peptide Library; D017433:Protein Structure, Secondary / Q000235:genetics*; D012276:Ricin / Q000235:genetics*
chemical_list: D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; D019151:Peptide Library; D012276:Ricin