title: Primary cutaneous anaplastic large cell lymphoma shows a distinct miRNA expression profile and reveals differences from tumor-stage mycosis fungoides.
abstract: The miRNA expression profiles of skin biopsies from 14 primary cutaneous anaplastic large cell lymphoma (C-ALCL) patients were analysed with miRNA microarrays using the same control group of 12 benign inflammatory dermatoses (BID) as previously used to study the miRNA expression profile of tumor-stage mycosis fungoides (MF). We identified 13 differentially expressed miRNAs between C-ALCL and BID. The up-regulation of miR-155, miR-27b, miR-30c and miR-29b in C-ALCL was validated by miRNA-Q-PCR on independent study groups. Additionally, the miRNA expression profiles of C-ALCL were compared with those of tumor-stage MF. Although miRNA microarray analysis did not identify statistically significant differentially expressed miRNAs, miRNA-Q-PCR demonstrated statistically significantly differential expression of miR-155, miR-27b, miR-93, miR-29b and miR-92a between tumor-stage MF and C-ALCL. This study, the first describing the miRNA expression profile of C-ALCL, reveals differences with tumor-stage MF, suggesting a different contribution to the pathogenesis of these lymphomas.
authors: Department of Dermatology, LUMC, Leiden, The Netherlands.
authors: Marchina F
authors: MF
authors: Benner
authors: Erica
authors: E
authors: Ballabio
authors: Marloes S
authors: MS
authors: van Kester
authors: Nigel J
authors: NJ
authors: Saunders
authors: Maarten H
authors: MH
authors: Vermeer
authors: Rein
authors: R
authors: Willemze
authors: Charles H
authors: CH
authors: Lawrie
authors: Cornelis P
authors: CP
authors: Tensen
mesh_terms: D001706:Biopsy; D020869:Gene Expression Profiling; D006801:Humans; D017728:Lymphoma, Large-Cell, Anaplastic / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D009182:Mycosis Fungoides / Q000378:metabolism* / Q000473:pathology; D009367:Neoplasm Staging; D012867:Skin / Q000473:pathology; D012871:Skin Diseases / Q000378:metabolism / Q000473:pathology; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D035683:MicroRNAs