title: Characterizing the effect of heavy metal contamination on marine mussels using metabolomics.
abstract: Marine mussels (Mytilus) are widely used as bioindicators to measure pollution in marine environments. In this study, (1)H NMR spectroscopy and multivariate statistical analyses were used to differentiate mussel groups from a heavy metal-polluted area (Onsan Bay) and a clean area (Dokdo area). Principal component analysis and orthogonal projection to latent structure-discriminant analysis revealed significant separation between extracts of mussels from Onsan Bay and from the Dokdo area. Organic osmolytes (betaine and taurine) and free amino acids (alanine, arginine, glutamine, phenylalanine, and threonine) were more highly accumulated in Onsan Bay mussels compared with Dokdo mussels. These results demonstrate that NMR-based metabolomics can be used as an efficient method for characterizing heavy metal contamination derived from polluted area compared to clean area and to identify metabolites related to environments that are contaminated with heavy metals.
authors: Seoul Center, Korea Basic Science Institute, Seoul 136-713, Republic of Korea.
authors: Yong-Kook
authors: YK
authors: Kwon
authors: Young-Sang
authors: YS
authors: Jung
authors: Jong-Chul
authors: JC
authors: Park
authors: Jungju
authors: J
authors: Seo
authors: Man-Sik
authors: MS
authors: Choi
authors: Geum-Sook
authors: GS
authors: Hwang
mesh_terms: D000818:Animals; D004784:Environmental Monitoring; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome / Q000502:physiology*; D055432:Metabolomics; D019216:Metals, Heavy / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D015999:Multivariate Analysis; D049878:Mytilus / Q000187:drug effects* / Q000378:metabolism; D025341:Principal Component Analysis; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*
chemical_list: D019216:Metals, Heavy; D014874:Water Pollutants, Chemical