title: Global changes in phospholipids identified by MALDI MS in rats with focal cerebral ischemia.
abstract: Neuronal membrane phospholipids are highly affected by oxidative stress caused by ischemic injury. Thus, it is necessary to identify key lipid components that show changes during ischemia to develop an effective approach to prevent brain damage from ischemic injury. The recent development of MALDI imaging MS (MALDI IMS) makes it possible to identify phospholipids that change between damaged and normal regions directly from tissues. In this study, we conducted IMS on rat brains damaged by ischemic injury and detected various phospholipids that showed unique distributions between normal and damaged areas of the brain. Among them, we confirmed changes in phospholipids such as lysophosphatidylcholine, phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin by MALDI IMS followed by MS/MS analysis. These lipids were present in high concentrations in the brain and are important for maintenance of cellular structure as well as production of second messengers for cellular signal transduction. Our results emphasize the identification of phospholipid markers for ischemic injury and successfully identified several distinctly located phospholipids in ischemic brain tissue.
authors: Department of Molecular Biotechnology, WCU Program,  Konkuk University, Seoul 143-701,  Korea.
authors: Selina Rahman
authors: SR
authors: Shanta
authors: Chang Soon
authors: CS
authors: Choi
authors: Jeong Hwa
authors: JH
authors: Lee
authors: Chan Young
authors: CY
authors: Shin
authors: Young Jun
authors: YJ
authors: Kim
authors: Kyun-Hwan
authors: KH
authors: Kim
authors: Kwang Pyo
authors: KP
authors: Kim
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism*; D002544:Cerebral Infarction / Q000378:metabolism; D015536:Down-Regulation; D008297:Male; D057054:Molecular Imaging; D008970:Molecular Weight; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
chemical_list: D010743:Phospholipids; D012964:Sodium; D011188:Potassium