title: Expression, purification, crystallization and preliminary crystallographic analysis of the phosphoglycerate kinase from Acinetobacter baumannii.
abstract: Acinetobacter baumannii is a common multidrug-resistant clinical pathogen that is often found in hospitals. The A. baumannii phosphoglycerate kinase (AbPGK) is involved in the key energy-producing pathway of glycolysis and presents a potential target for antibiotic development. AbPGK has been expressed and purified; it was crystallized using lithium sulfate as the precipitant. The AbPGK crystals belonged to space group P222(1). They diffracted to a resolution of 2.5 Å using synchrotron radiation at the Canadian Light Source.
authors: Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
authors: Kayla
authors: K
authors: Baretta
authors: Craig
authors: C
authors: Garen
authors: Jiang
authors: J
authors: Yin
authors: Michael N G
authors: MN
authors: James
mesh_terms: D040981:Acinetobacter baumannii / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D010735:Phosphoglycerate Kinase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D010735:Phosphoglycerate Kinase