title: An engineered E.coli strain for the production of glycoglycerolipids.
abstract: The glycolipid synthase MG517 from Mycoplasma genitalium catalyzes the glucosyl transfer from UDPGlc to diacylglycerol producing glycoglycerolipids (GGL) (Andrés et al., 2011). The enzyme was functional in E. coli accumulating GGL in the plasma membrane. A metabolic engineering strategy for GGL production was evaluated using this microorganism. To increase the levels of GGL precursors, UDPGlc and diacylglycerol, GalU and PlsC enzymes involved in their biosynthesis were overexpressed. Seven engineered strains were obtained containing different combinations of the mg517 with galU and plsC genes. Diacylglycerol synthesis showed to be limiting and the strain overexpressing MG517 and PlsC achieved the highest GGL yield. The new lipids were mono, di- and triglucosyldiacylglycerol with different acyl combinations in each compound. It indicates that the successive glucosyl transferase activities of MG517 have different acyl chain specificity for the acceptor substrate. GGL represented up to 6 mg per g of dry weight.
authors: Laboratory of Biochemistry, Bioengineering Department, Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, Barcelona, Spain.
authors: Neus
authors: N
authors: Mora-Buyé
authors: Magda
authors: M
authors: Faijes
authors: Antoni
authors: A
authors: Planas
mesh_terms: D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase / Q000096:biosynthesis / Q000235:genetics; D002462:Cell Membrane / Q000201:enzymology / Q000235:genetics; D004075:Diglycerides / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics; D006017:Glycolipids / Q000096:biosynthesis / Q000235:genetics; D008025:Ligases / Q000096:biosynthesis / Q000235:genetics; D060847:Metabolic Engineering; D045704:Mycoplasma genitalium / Q000201:enzymology / Q000235:genetics; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D004075:Diglycerides; D029968:Escherichia coli Proteins; D006017:Glycolipids; C015903:glycerolglycolipids; D051103:1-Acylglycerol-3-Phosphate O-Acyltransferase; C495091:plsC protein, E coli; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase; C121039:galU protein, E coli; D008025:Ligases