title: Description of high purity and high specific activity of [11C]Choline synthesis using TRACERlab FXc module, and detailed report of quality controls.
abstract: We proposed a method of synthesis to produce [11C]Choline using TRACERlab FXc module that utilized gas phase iodination. The product had radiochemical purity of 99.79 ± 0.14 % and specific activity of 45.7 ± 7.59 GBq/μmol. [11C]Choline did not have at the moment a specific monograph in European Pharmacopeia therefore we used, when possible, as quality controls reference the monograph of [18F]FDG and we proposed suitable methods to verify radiochemical purity and to quantify residual DMAE and choline amounts.
authors: Section of Biochemistry, Faculty of Medicine, University of Brescia, Viale Europa, 11, 25123 Brescia, Italy. biasiott@med.unibs.it
authors: Giorgio
authors: G
authors: Biasiotto
authors: Francesco
authors: F
authors: Bertagna
authors: Ugo
authors: U
authors: Biasiotto
authors: Carlo
authors: C
authors: Rodella
authors: Giovanni
authors: G
authors: Bosio
authors: Luigi
authors: L
authors: Caimi
authors: Giovanni
authors: G
authors: Bettinsoli
authors: Raffaele
authors: R
authors: Giubbini
mesh_terms: D002250:Carbon Radioisotopes; D060326:Chemistry Techniques, Synthetic / Q000295:instrumentation* / Q000592:standards; D002794:Choline / Q000138:chemical synthesis* / Q000737:chemistry; D004355:Drug Stability; D004731:Endotoxins / Q000032:analysis; D005740:Gases / Q000737:chemistry; D054879:Halogenation; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D011786:Quality Control; D011854:Radiochemistry / Q000295:instrumentation* / Q000592:standards; D012997:Solvents / Q000737:chemistry
chemical_list: D002250:Carbon Radioisotopes; D004731:Endotoxins; D005740:Gases; D012997:Solvents; D002794:Choline