title: Separation of patatins and protease inhibitors from potato fruit juice with clay minerals as cation exchangers.
abstract: Potato fruit juice as a by-product of the starch industry contains proteins with interesting functionalities such as protease inhibitors or patatin with its high nutritional value. Due to their functional properties, these proteins are principally of industrial interest. A drawback for the application of these potato proteins is the separation and isolation under maintenance of the biological activity. So far, there are no methods in literature, which are satisfying concerning the costs or the separation performance. In this study, we show a chromatographic approach using natural clay minerals as cation exchangers to separate two protein fractions in potato fruit juice. Additionally, the content of glycoalkaloids naturally occurring in potatoes is significantly reduced in a single step together with the separation of the patatins and the protease inhibitors.
authors: Institut für Biotechnologie, FG Bioverfahrenstechnik, Technische Universität Berlin, Berlin, Germany. kathrin.ralla@web.de
authors: Kathrin
authors: K
authors: Ralla
authors: Ulrich
authors: U
authors: Sohling
authors: Kirstin
authors: K
authors: Suck
authors: Cornelia
authors: C
authors: Kasper
authors: Friedrich
authors: F
authors: Ruf
authors: Thomas
authors: T
authors: Scheper
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D001628:Beverages / Q000032:analysis*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000302:isolation & purification*; D002411:Cation Exchange Resins / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D000077215:Clay; D005638:Fruit / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D011480:Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D000538:Aluminum Silicates; D002411:Cation Exchange Resins; D010940:Plant Proteins; D011480:Protease Inhibitors; C043222:patatin protein, Solanum tuberosum; D002265:Carboxylic Ester Hydrolases; D000077215:Clay