title: Antiamoebic properties of the actinomycete metabolites echinomycin A and tirandamycin A.
abstract: Entamoeba histolytica infects 50 million people per year, causing 100,000 deaths worldwide. The primary treatment for amoebiasis is metronidazole. However, increased pathogen resistance combined with the drug's toxic side effects encourages a search for alternative therapeutic agents. Secondary metabolites from marine bacteria are a promising resource for antiprotozoan drug discovery. In this study, extracts from a collection of marine-derived actinomycetes were screened for antiamoebic properties, and the activities of antibiotics echinomycin A and tirandamycin A are shown. Both antibiotics inhibited the in vitro growth of a E. histolytica laboratory strain (HM-1:IMSS) and a clinical isolate (Colombia, Col) at 30- to 60-μM concentrations. EIC(50) (estimated inhibitory concentration) values were comparable for both antibiotics (44.3-46.3 μM) against the E. histolytica clinical isolate.
authors: Department of Biology, Roger Williams University, One Old Ferry Road, Bristol, RI 02809, USA. aespinosa@rwu.edu
authors: Avelina
authors: A
authors: Espinosa
authors: Aaron M
authors: AM
authors: Socha
authors: Erica
authors: E
authors: Ryke
authors: David C
authors: DC
authors: Rowley
mesh_terms: D039903:Actinobacteria / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D000617:Aminoglycosides / Q000302:isolation & purification / Q000494:pharmacology*; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004448:Echinomycin / Q000302:isolation & purification / Q000494:pharmacology*; D004748:Entamoeba histolytica / Q000187:drug effects* / Q000302:isolation & purification; D004749:Entamoebiasis / Q000469:parasitology; D004783:Environmental Microbiology; D006801:Humans; D020128:Inhibitory Concentration 50; D008969:Molecular Sequence Data; D021261:Parasitic Sensitivity Tests; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000617:Aminoglycosides; D000981:Antiprotozoal Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C003608:tirandamycin A; D004448:Echinomycin