title: Delivery of PDGF-B and BMP-7 by mesoporous bioglass/silk fibrin scaffolds for the repair of osteoporotic defects.
abstract: Osteoporosis is a chronic disease affecting millions of people worldwide caused by an imbalance between bone-forming osteoblasts and bone-resorbing osteoclasts. Despite recent developments in pharmacological agents to prevent osteoporotic-related fractures, much less attention has been placed on the repair of bone defects following fracture. Critical to this process is the recruitment of mesenchymal stem cells (MSCs) to defect sites by growth factors. One method which has been effective for the sustained release of growth factors is that of gene therapy. The aim of the present study was to investigate newly developed mesoporous bioglass/silk fibrin scaffolds containing adPDGF-b and adBMP-7 into osteoporotic critical-sized femur defects in ovariectomised rats following treatment periods of 2 and 4 weeks. In vivo osteogenetic efficiency evaluated by μ-CT analysis, hematoxylin and eosin staining, and immunohistochemical (type I collagen, osteopontin and BSP) revealed significantly new bone formation in defects containing adenovirus for both PDGF-b and BMP-7 when compared to scaffolds alone and scaffolds containing BMP-7. TRAP-positive staining also demonstrated the ability for these scaffolds to be degraded over time and initiate bone turnover/remodeling. Although the use of gene therapy for clinical applications is still in its infancy, results from the present study demonstrate their potent ability to recruit mesenchymal progenitor cells through sustained release of PDGF-b and BMP-7 which may be beneficial for patients suffering from osteoporotic-related fractures.
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, PR China. zyf@whu.edu.cn
authors: Yufeng
authors: Y
authors: Zhang
authors: Ning
authors: N
authors: Cheng
authors: Richard
authors: R
authors: Miron
authors: Bin
authors: B
authors: Shi
authors: Xiangrong
authors: X
authors: Cheng
mesh_terms: D000256:Adenoviridae; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics*; D001861:Bone Regeneration; D002516:Ceramics / Q000737:chemistry; D005260:Female; D005269:Femur / Q000648:ultrastructure; D005349:Fibroins / Q000737:chemistry; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis; D010024:Osteoporosis / Q000628:therapy*; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; C022107:Bioglass; D055419:Bone Morphogenetic Protein 7; D020574:Proto-Oncogene Proteins c-sis; D005349:Fibroins