title: Wide range of interacting partners of pea Gβ subunit of G-proteins suggests its multiple functions in cell signalling.
abstract: Climate change is a major concern especially in view of the increasing global population and food security. Plant scientists need to look for genetic tools whose appropriate usage can contribute to sustainable food availability. G-proteins have been identified as some of the potential genetic tools that could be useful for protecting plants from various stresses. Heterotrimeric G-proteins consisting of three subunits Gα, Gβ and Gγ are important components of a number of signalling pathways. Their structure and functions are already well studied in animals but their potential in plants is now gaining attention for their role in stress tolerance. Earlier we have reported that over expressing pea Gβ conferred heat tolerance in tobacco plants. Here we report the interacting partners (proteins) of Gβ subunit of Pisum sativum and their putative role in stress and development. Out of 90 transformants isolated from the yeast-two-hybrid (Y2H) screening, seven were chosen for further investigation due to their recurrence in multiple experiments. These interacting partners were confirmed using β-galactosidase colony filter lift and ONPG (O-nitrophenyl-β-D-galactopyranoside) assays. These partners include thioredoxin H, histidine-containing phosphotransfer protein 5-like, pathogenesis-related protein, glucan endo-beta-1, 3-glucosidase (acidic isoform), glycine rich RNA binding protein, cold and drought-regulated protein (corA gene) and soluble inorganic pyrophosphatase 1. This study suggests the role of pea Gβ subunit in stress signal transduction and development pathways owing to its capability to interact with a wide range of proteins of multiple functions.
authors: Department of Botany, University of Delhi, India.
authors: Deepak
authors: D
authors: Bhardwaj
authors: Suman
authors: S
authors: Lakhanpaul
authors: Narendra
authors: N
authors: Tuteja
mesh_terms: D000222:Adaptation, Physiological; D057231:Climate Change; D044387:GTP-Binding Protein beta Subunits / Q000378:metabolism*; D009598:Nitrophenylgalactosides / Q000378:metabolism; D018532:Pisum sativum / Q000378:metabolism* / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism*; D015398:Signal Transduction; D013312:Stress, Physiological; D014170:Transformation, Genetic; D015003:Yeasts / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D044387:GTP-Binding Protein beta Subunits; D010940:Plant Proteins; C055012:2-nitrophenylgalactoside; D009598:Nitrophenylgalactosides; D001616:beta-Galactosidase