title: The shaker potassium channel is no target for xenon anesthesia in short-sleeping Drosophila melanogaster mutants.
abstract: BACKGROUND
  Xenon seems to be an ideal anesthetic drug. To explore if next to the antagonism at the NMDA-receptor other molecular targets are involved, we tested the xenon requirement in short sleeping Drosophila shaker mutants and in na[har(38)].
  
  
  METHODS
  The Drosophila melanogaster strains wildtype Canton-S, na[har(38)], sh(102) and sh(mns), were raised and sleep was measured. Based on the response of the flies at different xenon concentrations, logEC50 values were calculated.
  
  
  RESULTS
  The logEC50-values for WT Canton-S were 1.671 (1.601-1.742 95%-confidence intervall; n = 238; P versus sh(102) > 0,05), for sh(mns) 1.711 (1.650-1.773; n = 242; P versus WT Canton-S > 0,05). The logEC50-value for sh(102) was 1.594 (1.493-1.694; n = 261; P versus sh(mns) > 0.05). The logEC-value of na[har(38)] was 2.076 (1.619-2.532; n = 207; P versus sh(mns) < 0.05, versus sh(102) < 0.05, versus WT Canton-S < 0.05). P values for all shaker mutants were P > 0.05, while na[har(38)] was found to be hyposensitive compared to wildtype (P < 0.05).
  
  
  CONCLUSIONS
  The xenon requirement in Drosophila melanogaster is not influenced by a single gene mutation at the shaker locus, whereas a reduced expression of a nonselective cation channel leads to an increased xenon requirement. This supports the thesis that xenon mediates its effects not only via an antagonism at the NMDA-receptor.
authors: Department of Anaesthesiology and Operrative Intensive Care Medicine, University Hospital Schleswig-Holstein, Campus Kiel, Germany. christian.schaper@uksh.de
authors: C
authors: C
authors: Schaper
authors: J
authors: J
authors: Höcker
authors: R
authors: R
authors: Böhm
authors: T
authors: T
authors: Roeder
authors: B
authors: B
authors: Bein
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology; D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000502:physiology*; D009154:Mutation / Q000235:genetics; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D051637:Shaker Superfamily of Potassium Channels / Q000235:genetics*; D012890:Sleep / Q000187:drug effects* / Q000235:genetics*; D014978:Xenon / Q000494:pharmacology*
chemical_list: D018685:Anesthetics, Inhalation; D029721:Drosophila Proteins; D016194:Receptors, N-Methyl-D-Aspartate; C496929:Sh protein, Drosophila; D051637:Shaker Superfamily of Potassium Channels; D014978:Xenon