title: Up-regulation of cytochrome P450 and phase II enzymes by xenobiotics in precision-cut tissue slices.
abstract: 1.Induction of the drug-metabolising enzyme systems is a major cause of clinically relevant drug interactions. The potential of a drug to engage in such interactions may be assessed in in vitro systems such as precision-cut tissue slices. 2.Precision-cut tissue slices have a number of important advantages compared with the use of cells in culture, especially when tissues with a heterogeneous cellular population are concerned. 3.Extensive studies concerned with the induction of cytochrome P450 and Phase II enzymes in precision-cut tissue slices from various animal and human tissues, such as liver, lung and intestine, using established inducing agents have revealed that slices mimic the in vivo situation. 4.Precision-cut tissue slices is a reliable, informative and cost-effective technique for assessing the potential of chemicals to up-regulate drug-metabolising enzyme systems.
authors: Molecular Toxicology Group, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, UK. c.ioannides@surrey.ac.uk
authors: Costas
authors: C
authors: Ioannides
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D007422:Intestines / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008168:Lung / Q000201:enzymology*; D042282:Microdissection; D009924:Organ Culture Techniques / Q000379:methods; D015854:Up-Regulation / Q000187:drug effects*; D015262:Xenobiotics / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System