title: A regulatory role for IL-10 receptor signaling in development and B cell help of T follicular helper cells in mice.
abstract: IL -10 is widely accepted as a survival, proliferation, and differentiation factor for B cells. However, IL-10 deficiency accelerates disease progression as the result of autoantibody production in many autoimmune disease models. It was demonstrated that T follicular helper cells (T(FH) cells) play a key role in helping B cells that are secreting Abs. In this study, we demonstrated a regulatory role for IL-10R signaling on the development and B cell help function of T(FH) cells in vitro and in vivo. IL-1R subunit β-deficient (Il10rb(-/-)) Th cells were able to differentiate more readily into T(FH) cells, as well as secrete more IL-21 and IL-17 compared with wild-type Th cell-derived T(FH) cells. Increased IL-21 and IL-17 contributed to the enhanced B cell help functions of T(FH) cells. Further experiments demonstrated that IL-6 and IL-23 from dendritic cells in Il10rb(-/-) mice contributed to the differentiation of naive Th cells into T(FH) cells, as well as the generation of IL-21- and IL-17-producing T(FH) cells. Our results provide useful information for clarifying the immunoregulatory mechanisms associated with IL-10 deficiency in certain autoimmune disease models. This information could also be of benefit for the development of vaccines.
authors: Department of Laboratory Medicine, Shanghai Jiaotong University Medical School, Shanghai 200025, China.
authors: Gang
authors: G
authors: Cai
authors: Xiaomeng
authors: X
authors: Nie
authors: Weiwei
authors: W
authors: Zhang
authors: Beiying
authors: B
authors: Wu
authors: Jiafei
authors: J
authors: Lin
authors: Huaizhou
authors: H
authors: Wang
authors: Cen
authors: C
authors: Jiang
authors: Qian
authors: Q
authors: Shen
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000502:physiology; D008213:Lymphocyte Activation / Q000276:immunology; D008211:Lymphocyte Cooperation / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053701:Receptors, Interleukin-10 / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000502:physiology*; D015398:Signal Transduction / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000378:metabolism
chemical_list: C556062:IL10 protein, mouse; D053701:Receptors, Interleukin-10; D016753:Interleukin-10