title: Application of heptapeptides containing D-amino acid residues immobilized to magnetic particles and Sepharose for the study of binding properties of gastric aspartic proteases.
abstract: Synthetic heptapeptides containing D-amino acid residues and differing in the content of L-phenylalanine and L-tyrosine residues and their position (Val-D-Leu-Pro-Tyr-Phe-Val-D-Leu, Val-D-Leu-Pro-Tyr-Tyr-Val-D-Leu, Val-D-Leu-Pro-Phe-Tyr-Val-D-Leu) were immobilized to two types of carriers: glyoxal-activated magnetic agarose particles and CNBr-activated Sepharose. In both cases, peptides were immobilized via their terminal amino group. Immobilized peptides were used for the study of binding properties of two gastric aspartic proteases (porcine pepsin A and rat pepsin C). Porcine pepsin A was adsorbed to all studied peptide-modified magnetic carriers, while rat pepsin C interacted with immobilized ligands only slightly. Similar results were obtained in affinity chromatographic experiments using heptapeptides immobilized to Sepharose.
authors: Institute of Pathophysiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. misulka1@centrum.cz
authors: Michaela
authors: M
authors: Rajčanová
authors: Marie
authors: M
authors: Tichá
authors: Zdenka
authors: Z
authors: Kučerová
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D005753:Gastric Mucosa / Q000737:chemistry / Q000201:enzymology*; D007700:Kinetics; D008280:Magnetics; D010434:Pepsin A / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D012685:Sepharose / Q000737:chemistry*; D013552:Swine
chemical_list: D010455:Peptides; D012685:Sepharose; D010434:Pepsin A; C020557:gastricsin