title: MS analysis reveals O-methylation of L-lactate dehydrogenase from pancreatic ductal adenocarcinoma cells.
abstract: L-lactate dehydrogenase (LDH) converts pyruvate to lactate when oxygen is absent or in short supply, and the enzyme plays a crucial role in cancer metabolism. The functions of many mammalian proteins are modulated by posttranslational modifications (PTMs), and it has been reported that LDH was subjected to several PTMs, including phosphorylation, acetylation, and methylation. In this present work, we characterized the PTMs of LDH from pancreatic ductal adenocarcinoma (PDAC) cells by electrophoresis and mass spectrometry, and identified 13 O-methylated residues from the enzyme. In addition, our qualitative analysis revealed differential methylation of LDH from normal duct cells. The preliminary findings from this study provide important biochemical information toward further understanding of the LDH modifications and their functional significance in pathophysiological processes of pancreatic cancer.
authors: Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA 20110, USA. wzhou@gmu.edu
authors: Weidong
authors: W
authors: Zhou
authors: Michela
authors: M
authors: Capello
authors: Claudia
authors: C
authors: Fredolini
authors: Leda
authors: L
authors: Racanicchi
authors: Lorenzo
authors: L
authors: Piemonti
authors: Lance A
authors: LA
authors: Liotta
authors: Francesco
authors: F
authors: Novelli
authors: Emanuel F
authors: EF
authors: Petricoin
mesh_terms: D000595:Amino Acid Sequence; D021441:Carcinoma, Pancreatic Ductal / Q000737:chemistry / Q000201:enzymology*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000737:chemistry*; D008745:Methylation; D008969:Molecular Sequence Data; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000201:enzymology*; D020033:Protein Isoforms / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D020033:Protein Isoforms; D007770:L-Lactate Dehydrogenase