title: Acidomonas methanolica-associated necrotizing lymphadenitis in a patient with chronic granulomatous disease.
abstract: OBJECTIVE
  Adenitis for which no causative organism can be isolated is a common occurrence in patients with chronic granulomatous disease (CGD). Here we identify Acidomonas methanolica as a pathogen associated with adenitis in a patient with CGD.
  
  
  METHODS
  The causative pathogen was obtained after prolonged incubation of an excised lymph node in thioglycolate broth. Identification was carried out by sequencing the 16s rRNA. Immunoblots were prepared utilizing protein extracts from the case patient's A. methanolica isolate, an ATCC type strain of A. methanolica and G. bethesdensis.
  
  
  RESULTS
  Fastidious gram-negative rods grew after prolonged incubation of an excised lymph node in thioglycolate broth. Sequencing of the 16s rRNA identified the organism as A. methanolica. Immunoblot confirmed the pathogen's role in the patient's adenitis by showing the patient's specific immune response to the organism.
  
  
  CONCLUSIONS
  A. methanolica is the second member of the family, Acetobacteaceae to be associated with adenitis in patients with CGD.
authors: Division of General Pediatrics, MS#75, Children's Hospital Los Angeles and Keck School of Medicine, University of Southern California, 4650 Sunset Blvd., Los Angeles, CA 90027, USA. chasejm@gmail.com
authors: John M
authors: JM
authors: Chase
authors: Steven M
authors: SM
authors: Holland
authors: David E
authors: DE
authors: Greenberg
authors: Kimberly
authors: K
authors: Marshall-Batty
authors: Adrian M
authors: AM
authors: Zelazny
authors: Joseph A
authors: JA
authors: Church
mesh_terms: D016947:Acetobacteraceae / Q000235:genetics* / Q000302:isolation & purification; D002648:Child; D006105:Granulomatous Disease, Chronic / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D020042:Histiocytic Necrotizing Lymphadenitis / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology* / Q000473:pathology; D008297:Male; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S