title: Structure of the RBD-PRDI fragment of the antiterminator protein GlcT.
abstract: GlcT is a transcriptional antiterminator protein that is involved in regulation of glucose metabolism in Bacillus subtilis. Antiterminator proteins bind specific RNA sequences, thus preventing the formation of overlapping terminator stem-loops. The structure of a fragment (residues 3-170) comprising the RNA-binding domain (RBD) and the first regulatory domain (PRDI) of GlcT was solved at 2.0 Å resolution with one molecule in the asymmetric unit. The two domains are connected by a helical linker. Their interface is mostly constituted by hydrophobic interactions.
authors: Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors: Sebastian
authors: S
authors: Himmel
authors: Christian
authors: C
authors: Grosse
authors: Sebastian
authors: S
authors: Wolff
authors: Claudia
authors: C
authors: Schwiegk
authors: Stefan
authors: S
authors: Becker
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000737:chemistry*; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; C115000:GlcT protein, Bacillus subtilis; D010446:Peptide Fragments; D016601:RNA-Binding Proteins; D014157:Transcription Factors