title: Evaluation of immunogenicity of purified cell wall-associated 34 kDa antigen of Mycobacterium avium subsp. paratuberculosis infection.
abstract: The 34 kDa cell wall protein of Mycobacterium avium subsp. paratuberculosis (MAP) has been suggested as a major species-specific immunodominant antigen in Johne's disease. However to date, there has not been a purified 34 kDa protein isolated from bacterial lysates used in immunogenicity analysis. Therefore we attempted to assess the immunogenicity properties of the purified cell wall 34 kDa protein for the first time, and compare the results with previous studies. We used an ELISA test for evaluation of the immunogenicity of this 34 kDa antigen against MAP infection. All serum samples from cattle confirmed to be infected with MAP were positive and those from healthy cattle were negative with the present antigen in ELISA tests. The sensitivity and specificity of 34 kDa antigen were then evaluated in comparison with a standard commercial kit and whole cell wall extracts. The results indicated that the pure 34 kDa antigen specific to MAP with high specificity and sensitivity has a strong potential for use in serodiagnosis assays and screening of Johne's disease.
authors: Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karadj, Iran.
authors: Narges
authors: N
authors: Kavid
authors: Rasool
authors: R
authors: Madani
authors: Saman
authors: S
authors: Hosseinkhani
authors: Nader
authors: N
authors: Mosavari
authors: Fariba
authors: F
authors: Golchinfar
authors: Tara
authors: T
authors: Emami
authors: Roohallah
authors: R
authors: Keshavarz
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D002473:Cell Wall; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology* / Q000378:metabolism; D010283:Paratuberculosis / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins