title: Optimization of enzymatic hydrolysis of cassava to obtain fermentable sugars.
abstract: This work evaluates the enzymatic hydrolysis of starch from cassava using pectinase, α-amylase, and amyloglucosidase. A central composite rotational design (CCRD) was carried out to evaluate the effects of amyloglucosidase, pectinase, reaction time, and solid to liquid ratio. All the experiments were carried out in a bioreactor with working volume of 2 L. Approximately 98% efficiency hydrolysis was obtained, resulting in a concentration of total reducing sugar released of 160 g/L. It was concluded that pectinase improved the hydrolysis of starch from cassava. Reaction time was found to be significant until 7 h of reaction. A solid to liquid ratio of 1.0 was considered suitable for hydrolysis of starch from cassava. Amyloglucosidase was a significant variable in the process: after its addition to the reaction media, a 30%-50% increase in the amount of total reducing sugar released was observed. At optimal conditions the maximum productivity obtained was 22.9 g/(L·h).
authors: Department of Chemical Engineering, Federal University of Santa Maria, Brazil.
authors: Renata M
authors: RM
authors: Collares
authors: Luiza V S
authors: LV
authors: Miklasevicius
authors: Mariana M
authors: MM
authors: Bassaco
authors: Nina P G
authors: NP
authors: Salau
authors: Marcio A
authors: MA
authors: Mazutti
authors: Dilson A
authors: DA
authors: Bisognin
authors: Lisiane M
authors: LM
authors: Terra
mesh_terms: D019149:Bioreactors; D002241:Carbohydrates / Q000737:chemistry*; D004789:Enzyme Activation; D004867:Equipment Design; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry*; D006868:Hydrolysis; D002365:Manihot / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D010936:Plant Extracts; D005087:Glucan 1,4-alpha-Glucosidase