title: Sub-viral imaging of vaccinia virus using super-resolution microscopy.
abstract: The study of host-pathogen interactions over past decades has benefited from advances in microscopy and fluorescent imaging techniques. A particularly powerful model in this field is vaccinia virus (VACV), which due to its amenability to genetic manipulation has been a productive model in advancing the understanding of the transport of subcellular cargoes. Conventional light microscopy imposes an upper limit of resolution of ~250nm, hence knowledge of events occurring at the sub-viral resolution is based predominantly on studies utilising electron microscopy. The development of super-resolution light microscopy presents the opportunity to bridge the gap between these two technologies. This report describes the analysis of VACV replication using fluorescent recombinant viruses, achieving sub-viral resolution with three-dimensional structured illumination microscopy. This is the first report of successfully resolving poxvirus particle morphologies at the scale of single virus particles using light microscopy.
authors: School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia.
authors: Jacquelyn
authors: J
authors: Horsington
authors: Lynne
authors: L
authors: Turnbull
authors: Cynthia B
authors: CB
authors: Whitchurch
authors: Timothy P
authors: TP
authors: Newsome
mesh_terms: D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D014616:Vaccinia virus / Q000648:ultrastructure*