title: Overexpression of histone deacetylase 6 contributes to accelerated migration             and invasion activity of hepatocellular carcinoma cells.
abstract: Histone deacetylase 6 (HDAC6) is a cytoplasmic enzyme that regulates many             important biological processes, including cell migration, viral infection and             autophagy. The aim of this study was to investigate the significance of HDAC6             in the invasion and metastasis activities of hepatocellular carcinoma (HCC). Three             HCC cell lines and two primary cultures of hepatocytes were used for biological             experiments. Immunohistochemistry for HDAC6 protein was also examined in 70 resected             primary HCCs. Knockdown of the HDAC6 gene in the HCC cell lines was carried out             by treatment with siRNA, and their migration and invasion activities were examined             by the scratch assay and Matrigel invasion assay, respectively. HDAC6 expression             was greater in all of the HCC cell lines compared to the primary cultures of hepatocytes.             Knockdown of HDAC6 markedly downregulated the migration and invasion activities             of all HCC cell lines (P<0.05). Overexpression of HDAC6 protein to a level             higher than that in the corresponding normal hepatocytes was observed in 14 (20%)             of the 70 primary HCCs, and was significantly correlated with high clinical stage,             number of tumors, vascular invasion and intrahepatic metastasis (P<0.05). These             results suggest that overexpression of the HDAC6 protein is involved in the migration             and invasion activities of HCC cells, and may be a good biomarker for prediction             of intrahepatic metastasis.
authors: Department of Tumor Biology, Institute of Biomedical Science, School of Medicine, Iwate Medical University, Morioka, Japan.
authors: Kiminori
authors: K
authors: Kanno
authors: Shoji
authors: S
authors: Kanno
authors: Hiroyuki
authors: H
authors: Nitta
authors: Noriyuki
authors: N
authors: Uesugi
authors: Tamostu
authors: T
authors: Sugai
authors: Tomoyuki
authors: T
authors: Masuda
authors: Go
authors: G
authors: Wakabayashi
authors: Chihaya
authors: C
authors: Maesawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D034622:RNA Interference
chemical_list: C433811:HDAC6 protein, human; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases