title: Copper-induced intracellular calcium release requires extracellular calcium entry and activation of L-type voltage-dependent calcium channels in Ulva compressa.
abstract: The marine alga Ulva compressa exposed to 10 µM copper showed a triphasic increase of intracellular calcium with maximal levels at 2, 3 and 12 h involving the activation of ryanodine-, Ins(1,4,5)P3- and NAADP-sensitive calcium channels. In order to analyze the requirement of extracellular calcium entry for intracellular calcium release as well as the activation of voltage-dependent calcium channels (VDCC) and phospholipase C, U. compressa was treated with EGTA, a non-permeable calcium chelating agent, with verapamil, nipfedipine and diltiazem, inhibitors of L-type VDCC, and with neomycin and U731222, inhibitors of phospholipase C. The release of intracellular calcium was partially inhibited with EGTA at 2 and 3 h and completely inhibited at 12 h of copper exposure and decreased with inhibitors of L-type VDCC and phospholipase C. Thus, copper-induced intracellular calcium release depends on calcium entry and activation of L-type VDCC and phospholipase C. An integrative model of copper-induced cellular responses in U. compressa is presented.
authors: Universidad de Santiago de Chile, Departamento de Biología, Santiago, Chile.
authors: Alberto
authors: A
authors: González
authors: María de los Angeles
authors: Mde L
authors: Cabrera
authors: Macarena
authors: M
authors: Mellado
authors: Susana
authors: S
authors: Cabello
authors: Seabastián
authors: S
authors: Márquez
authors: Bernardo
authors: B
authors: Morales
authors: Alejandra
authors: A
authors: Moenne
mesh_terms: D002118:Calcium / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D003300:Copper / Q000494:pharmacology*; D004110:Diltiazem / Q000494:pharmacology; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism*; D015640:Ion Channel Gating / Q000187:drug effects*; D008954:Models, Biological; D009355:Neomycin / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology; D044445:Ulva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014700:Verapamil / Q000494:pharmacology
chemical_list: D020746:Calcium Channels, L-Type; D003300:Copper; D014700:Verapamil; D004110:Diltiazem; D009355:Neomycin; D009543:Nifedipine; D002118:Calcium