title: Exploring the natural fungal biodiversity of tropical and temperate forests toward improvement of biomass conversion.
abstract: In this study, natural fungal diversity in wood-decaying species was explored for biomass deconstruction. In 2007 and 2008, fungal isolates were collected in temperate forests mainly from metropolitan France and in tropical forests mainly from French Guiana. We recovered and identified 74 monomorph cultures using morphological and molecular identification tools. Following production of fungal secretomes under inductive conditions, we evaluated the capacity of these fungal strains to potentiate a commercial Trichoderma reesei cellulase cocktail for the release of soluble sugars from biomass. The secretome of 19 isolates led to an improvement in biomass conversion of at least 23%. Of the isolates, the Trametes gibbosa BRFM 952 (Banque de Ressources Fongiques de Marseille) secretome performed best, with 60% improved conversion, a feature that was not universal to the Trametes and related genera. Enzymatic characterization of the T. gibbosa BRFM 952 secretome revealed an unexpected high activity on crystalline cellulose, higher than that of the T. reesei cellulase cocktail. This report highlights the interest in a systematic high-throughput assessment of collected fungal biodiversity to improve the enzymatic conversion of lignocellulosic biomass. It enabled the unbiased identification of new fungal strains issued from biodiversity with high biotechnological potential.
authors: INRA, UMR1163 BCF, Marseille, France. jean-guy.berrin@univ-amu.fr
authors: Jean-Guy
authors: JG
authors: Berrin
authors: David
authors: D
authors: Navarro
authors: Marie
authors: M
authors: Couturier
authors: Caroline
authors: C
authors: Olivé
authors: Sacha
authors: S
authors: Grisel
authors: Mireille
authors: M
authors: Haon
authors: Sabine
authors: S
authors: Taussac
authors: Christian
authors: C
authors: Lechat
authors: Régis
authors: R
authors: Courtecuisse
authors: Anne
authors: A
authors: Favel
authors: Pedro M
authors: PM
authors: Coutinho
authors: Laurence
authors: L
authors: Lesage-Meessen
mesh_terms: D044822:Biodiversity; D018533:Biomass; D044602:Cellulases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005602:France; D005616:French Guiana; D005658:Fungi / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D005947:Glucose / Q000378:metabolism; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014197:Trees / Q000382:microbiology*; D014329:Tropical Climate; D014934:Wood / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D044602:Cellulases; D005947:Glucose