title: HSV-mediated gene transfer of C3 transferase inhibits Rho to promote axonal regeneration.
abstract: Although surgical re-implantation of spinal roots may improve recovery of proximal motor function after cervical root avulsion, recovery of sensory function necessary for fine motor coordination of the hand has been difficult to achieve, in large part because of failure of regeneration of axons into the spinal cord. In order to enhance regeneration, we constructed a non-replicating herpes simplex virus (HSV)-vector carrying the gene coding for bacterial C3 transferase (C3t). Subcutaneous inoculation of the vector into the skin of the forepaw 1 week after a dorsal C5-T1 rhizotomy resulted in expression of C3t in dorsal root ganglion (DRG) neurons and inhibition of Rho GTPase activity, resulting in extensive axonal regeneration into the spinal cord that correlated with improved sensory-motor coordination of the forepaw.
authors: Department of Neurology, University of Michigan and VA Ann Arbor Healthcare System, Ann Arbor, MI, USA
authors: Zhigang
authors: Z
authors: Zhou
authors: Xiangmin
authors: X
authors: Peng
authors: Peipei
authors: P
authors: Chiang
authors: Jeeyong
authors: J
authors: Kim
authors: Xiankui
authors: X
authors: Sun
authors: David J
authors: DJ
authors: Fink
authors: Marina
authors: M
authors: Mata
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics*; D000818:Animals; D001369:Axons / Q000502:physiology*; D001905:Botulinum Toxins / Q000235:genetics*; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D018014:Gene Transfer Techniques; D057809:HEK293 Cells; D006801:Humans; D009416:Nerve Regeneration / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D018139:Simplexvirus / Q000502:physiology*; D054460:rho-Associated Kinases / Q000235:genetics
chemical_list: D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D054460:rho-Associated Kinases; D001905:Botulinum Toxins