title: In situ Raman spectroscopy for simultaneous monitoring of multiple process parameters in mammalian cell culture bioreactors.
abstract: In this study, the application of Raman spectroscopy to the simultaneous quantitative determination of glucose, glutamine, lactate, ammonia, glutamate, total cell density (TCD), and viable cell density (VCD) in a CHO fed-batch process was demonstrated in situ in 3 L and 15 L bioreactors. Spectral preprocessing and partial least squares (PLS) regression were used to correlate spectral data with off-line reference data. Separate PLS calibration models were developed for each analyte at the 3 L laboratory bioreactor scale before assessing its transferability to the same bioprocess conducted at the 15 L pilot scale. PLS calibration models were successfully developed for all analytes bar VCD and transferred to the 15 L scale.
authors: School of Chemical and Bioprocess Engineering, University College Dublin, Dublin 4, Ireland.
authors: Jessica
authors: J
authors: Whelan
authors: Stephen
authors: S
authors: Craven
authors: Brian
authors: B
authors: Glennon
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D016466:CHO Cells; D002452:Cell Count; D002477:Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D006224:Cricetinae; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D018698:Glutamic Acid / Q000032:analysis / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D019344:Lactic Acid; D018698:Glutamic Acid; D005947:Glucose