title: [Effect of NR2E1 on the division and proliferation of neuroblastoma cells].
abstract: OBJECTIVE
  To explore the effects of nuclear protein-like transcription factor nuclear receptor subfamily 2 group E member 1 (NR2E1) on the growth, division, and proliferation of neuroblastoma cell line IMR32.
  
  
  METHODS
  A NR2E1 shiRNA plasmid vector was constructed and transfected into neuroblastoma cell line IMR32 using lipofedamine™2000. Subsequent cell growth was measured by cell counting and the protein expression of somatic nuclear division was examined by immunofluorescent staining.
  
  
  RESULTS
  At 48 h after the neuroblastoma cells IMR32 were transfected with NR2E1-shiRNA vector, the related nuclear division protein and the proliferation of the transfected cells IMR32 were remarkably depressed.
  
  
  CONCLUSIONS
  Cells division and proliferation of neuroblastoma cell line IMR32 is inhibited through transfection with the NR2E1-shiRNA plasmid vector.
authors: Department of Neurology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors: Haixia
authors: H
authors: Zhu
authors: Nan
authors: N
authors: Ma
authors: Chunhong
authors: C
authors: Jiao
authors: Weiwen
authors: W
authors: Xue
authors: Zhaojun
authors: Z
authors: Zeng
mesh_terms: D002455:Cell Division / Q000235:genetics* / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology*; D057093:Orphan Nuclear Receptors; D034741:RNA, Small Interfering / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: C088601:NR2E1 protein, human; D057093:Orphan Nuclear Receptors; D034741:RNA, Small Interfering; D018160:Receptors, Cytoplasmic and Nuclear