title: DNA detection on lateral flow test strips: enhanced signal sensitivity using LNA-conjugated gold nanoparticles.
abstract: A lateral flow test strip assay, enabling sensitive detection of DNA specific to the foodborne pathogen E. coli O157:H7, is described. The use of LNA-conjugated gold nanoparticle probes, along with signal amplification protocols, results in minimum detectable concentrations of ~0.4 nM.
authors: Biosensors and Nanotechnology Applications Laboratory, University of Idaho, Coeur d'Alene, ID 83814-2277, USA. srastogi@uidaho.edu
authors: Shiva K
authors: SK
authors: Rastogi
authors: CharLene M
authors: CM
authors: Gibson
authors: Josh R
authors: JR
authors: Branen
authors: D Eric
authors: DE
authors: Aston
authors: A Larry
authors: AL
authors: Branen
authors: Patrick J
authors: PJ
authors: Hrdlicka
mesh_terms: D015374:Biosensing Techniques; D004269:DNA, Bacterial / Q000737:chemistry*; D019453:Escherichia coli O157 / Q000737:chemistry / Q000302:isolation & purification*; D005516:Food Microbiology; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D011934:Reagent Strips / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D009841:Oligonucleotides; D011934:Reagent Strips; C477371:locked nucleic acid; D006046:Gold