title: Overexpression of FABP3 promotes apoptosis through inducing mitochondrial impairment in embryonic cancer cells.
abstract: Fatty acid-binding protein 3 (FABP3) is a low-molecular-weight protein with a distinct tissue distribution that may play an important role in fatty acid transport, cell growth, cellular signaling, and gene transcription. Previously, we have found that FABP3 was involved in apoptosis-associated congenital cardiac malformations, but the underlying mechanisms have not yet been described. In the present study, we investigated the characteristics of mitochondrial dysfunction in embryonic cancer cells (P19 cells) that overexpressed FABP3. We demonstrated that in FABP3-overexpressing P19 cells a lower cellular ATP production was accompanied by a dramatic decrease in mitochondrial membrane potential (MMP), despite the lack of a substantial decrease in the mtDNA copy number. In addition, FABP3 overexpression also led to an imbalance in mitochondrial dynamics and to excess intracellular reactive oxygen species production. Collectively, our results indicated that overexpression of FABP3 in P19 cells caused mitochondrion dysfunction that might be responsible for the development of FABP3-induced apoptosis.
authors: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, People's Republic of China.
authors: Gui Xian
authors: GX
authors: Song
authors: Ya Hui
authors: YH
authors: Shen
authors: Yao Qiu
authors: YQ
authors: Liu
authors: Wei
authors: W
authors: Sun
authors: Li Ping
authors: LP
authors: Miao
authors: Li Juan
authors: LJ
authors: Zhou
authors: Hai Lang
authors: HL
authors: Liu
authors: Rong
authors: R
authors: Yang
authors: Xiang Qing
authors: XQ
authors: Kong
authors: Ke Jiang
authors: KJ
authors: Cao
authors: Ling Mei
authors: LM
authors: Qian
authors: Yan Hui
authors: YH
authors: Sheng
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002470:Cell Survival; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism / Q000473:pathology*; D054278:Embryonal Carcinoma Stem Cells / Q000473:pathology*; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D063154:Mitochondrial Dynamics; D053142:Mitochondrial Size; D010084:Oxidation-Reduction; D055550:Protein Stability; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004272:DNA, Mitochondrial; C493409:Fabp3 protein, mouse; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate