title: Self-assembly of tunable protein suprastructures from recombinant oleosin.
abstract: Using recombinant amphiphilic proteins to self-assemble suprastructures would allow precise control over surfactant chemistry and the facile incorporation of biological functionality. We used cryo-TEM to confirm self-assembled structures from recombinantly produced mutants of the naturally occurring sunflower protein, oleosin. We studied the phase behavior of protein self-assembly as a function of solution ionic strength and protein hydrophilic fraction, observing nanometric fibers, sheets, and vesicles. Vesicle membrane thickness correlated with increasing hydrophilic fraction for a fixed hydrophobic domain length. The existence of a bilayer membrane was corroborated in giant vesicles through the localized encapsulation of hydrophobic Nile red and hydrophilic calcein. Circular dichroism revealed that changes in nanostructural morphology in this family of mutants was unrelated to changes in secondary structure. Ultimately, we envision the use of recombinant techniques to introduce novel functionality into these materials for biological applications.
authors: Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors: Kevin B
authors: KB
authors: Vargo
authors: Ranganath
authors: R
authors: Parthasarathy
authors: Daniel A
authors: DA
authors: Hammer
mesh_terms: D002942:Circular Dichroism; D020285:Cryoelectron Microscopy; D016503:Drug Delivery Systems / Q000379:methods; D005452:Fluoresceins; D018613:Microscopy, Confocal; D009154:Mutation / Q000235:genetics; D010078:Oxazines; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D022161:Transport Vesicles / Q000378:metabolism*
chemical_list: D005452:Fluoresceins; D010078:Oxazines; D010940:Plant Proteins; D011994:Recombinant Proteins; C079350:oleosin, Helianthus annuus; C044808:nile red; C007740:fluorexon