title: [A study of repairing mandibular defect using tissue engineering bone with bone marrow stem cells cell sheets in dog].
abstract: OBJECTIVE
  To reconstruct mandibular defect using tissue engineering bone with bone marrow stem cells (BMSCs) cell sheets and investigate the effect of cell sheets on osteogenesis.
  
  
  METHODS
  BMSCs were isolated with the method of density gradient centrifugation from canine and cultured. BMSCs were induced to differentiate to osteoblasts. BMSCs induced were fabricated to BMSCs cell sheets. The poly (lactic-co-glycolic acid) (PLGA) wrapped with cell sheets were implanted into the mandibular defect in the left side (experimental side). PLGA wrapped without cell sheets were implanted into the right side (control side) of mandibles. 16 dogs were evenly divided into 4 groups, and one group of them was executed in 4, 8, 12, 16 weeks for gross investigation and histological observation.
  
  
  RESULTS
  The osteogenesis of experimental side was better than that of control side. 16 weeks after implantation, most areas of the mandibular defect were replaced by fresh bone tissue. Compact bone similar to normal bone tissue formed in the lingual defect of mandible and had bony union with the bone stump. The optical density of the fresh bone in the experimental side was higher than that of the control side, there was a significant difference between the two methods (P<0.05). Plenty of lamellar bones formed in experimental side and Haversian system, as well as red marrow, were observed.
  
  
  CONCLUSIONS
  Tissue engineering bone with the structure of lamellar bones can be formed by the technology of BMSCs cell sheets.
authors: Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China.
authors: Chao
authors: C
authors: Yao
authors: Lingxue
authors: L
authors: Bu
authors: Ke
authors: K
authors: Wang
authors: Ningyi
authors: N
authors: Li
authors: Lingling
authors: L
authors: Wang
authors: Yueyuan
authors: Y
authors: Yu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D001842:Bone and Bones; D004285:Dogs; D019344:Lactic Acid; D008334:Mandible; D010006:Osteoblasts; D010012:Osteogenesis; D011091:Polyesters; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D023822:Tissue Engineering
chemical_list: D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide)