title: MiR-410 regulates MET to influence the proliferation and invasion of glioma.
abstract: MET, the receptor for hepatocyte growth factor receptor (HGF), has been reported to trigger multiple and sometimes opposing cellular responses in various types of tumor cells. It has been implicated in the regulation of tumor-cell survival, proliferation, angiogenesis, invasion and metastasis. However, the MET regulatory mechanism in glioma is not well known. MicroRNAs are a class of small noncoding RNAs that play important roles in a variety of biological processes including human cancers. In this study, we used computational and expressional analysis to identify that the 'seed sequence' of miR-410 matched the 3' UTR of the MET mRNA. Besides, the expression of miR-410 was inversely associated with MET in human glioma tissues. Using luciferase and western blot assay, we certified that miR-410 directly targeted MET in glioma cells. While restoring expression of miR-410 led to proliferation inhibition and reduced invasive capability in glioma cells. Furthermore, we showed that miR-410 played an important role in regulating MET-induced AKT signal transduction. While downregulation of MET by RNAi, we observed that MET knockdown resulted in effects similar to that with miR-410 transfection in glioma cells. Our findings suggest that miR-410, a direct regulator of MET, may function as a tumor suppressor in human gliomas.
authors: Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, PR China.
authors: Lingchao
authors: L
authors: Chen
authors: Junhe
authors: J
authors: Zhang
authors: Yan
authors: Y
authors: Feng
authors: Ruiyan
authors: R
authors: Li
authors: Xu
authors: X
authors: Sun
authors: Wenzhong
authors: W
authors: Du
authors: Xinyin
authors: X
authors: Piao
authors: Hanbing
authors: H
authors: Wang
authors: Dongbo
authors: D
authors: Yang
authors: Ying
authors: Y
authors: Sun
authors: Xianfeng
authors: X
authors: Li
authors: Tao
authors: T
authors: Jiang
authors: Chunsheng
authors: C
authors: Kang
authors: Yongli
authors: Y
authors: Li
authors: Chuanlu
authors: C
authors: Jiang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D000970:Antineoplastic Agents; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D000970:Antineoplastic Agents; C568527:MIRN410 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C491743:MET protein, human; D019859:Proto-Oncogene Proteins c-met; D051057:Proto-Oncogene Proteins c-akt