title: The influenza fingerprints: NS1 and M1 proteins contribute to specific host cell ultrastructure signatures upon infection by different influenza A viruses.
abstract: Influenza A are nuclear replicating viruses which hijack host machineries in order to achieve optimal infection. Numerous functional virus-host interactions have now been characterized, but little information has been gathered concerning their link to the virally induced remodeling of the host cellular architecture. In this study, we infected cells with several human and avian influenza viruses and we have analyzed their ultrastructural modifications by using electron and confocal microscopy. We discovered that infections lead to a major and systematic disruption of nucleoli and the formation of a large number of diverse viral structures showing specificity that depended on the subtype origin and genomic composition of viruses. We identified NS1 and M1 proteins as the main actors in the remodeling of the host ultra-structure and our results suggest that each influenza A virus strain could be associated with a specific cellular fingerprint, possibly correlated to the functional properties of their viral components.
authors: Equipe VirCell, Laboratoire de Virologie et Pathologie Humaine, VirPath EMR 4610, Université de Lyon, Université Claude Bernard Lyon 1, Hospices Civils de Lyon, Faculté de médecine RTH Laennec, rue Guillaume Paradin, F-69008 Lyon, France.
authors: Olivier
authors: O
authors: Terrier
authors: Vincent
authors: V
authors: Moules
authors: Coralie
authors: C
authors: Carron
authors: Gaëlle
authors: G
authors: Cartet
authors: Emilie
authors: E
authors: Frobert
authors: Matthieu
authors: M
authors: Yver
authors: Aurelien
authors: A
authors: Traversier
authors: Thorsten
authors: T
authors: Wolff
authors: Beatrice
authors: B
authors: Riteau
authors: Nadia
authors: N
authors: Naffakh
authors: Bruno
authors: B
authors: Lina
authors: Jean-Jacques
authors: JJ
authors: Diaz
authors: Manuel
authors: M
authors: Rosa-Calatrava
mesh_terms: D000818:Animals; D002460:Cell Line; D003593:Cytoplasm / Q000648:ultrastructure*; D054884:Host-Pathogen Interactions; D006801:Humans; D009980:Influenza A virus / Q000472:pathogenicity*; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D015388:Organelles / Q000648:ultrastructure*; D014763:Viral Matrix Proteins / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: C085820:INS1 protein, influenza virus; C488936:M1 protein, Influenza A virus; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins