title: [Screening of potential proteins from patients with idiopathic pulmonary fibrosis by using of surface enhanced laser desorption/ionization time of flight mass spectrum].
abstract: OBJECTIVE
  To screen potential proteins in bronchoalveolar lavage fluid (BALF) and serum samples obtained from patients with idiopathic pulmonary fibrosis (IPF), for the purpose of discovering candidate  biomarkers.
  
  
  METHODS
  BALF and serum samples from 34 patients diagnosed IPF (IPF group) and 25 non-smoker healthy controls (control group) were collected. Surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) was used to obtain protein fingerprints from BALF and serum samples. Biomarker Wizard and Biomarker Pattern were introduced in bioinformatics analysis.
  
  
  RESULTS
  A total of 247 protein peaks were detected in BALF, and 13 peaks with statistical difference compared with control group. Among 13 detected protein peaks, 7 with mass/charge ratio (M/Z) values of 2240.57, 3459.32, 3501.78, 4146.50, 4516.51, 4615.88, 5651.26 were   statistically   up-regulated  (all  P < 0.01);  and  6  peaks  with  M/Z  values  of  4989.91, 5043.68, 6968.76, 11 687.70, 11 782.10, 14 733.30 were significantly down-regulated (all P < 0.01). In addition, 142 protein peaks were differentially expressed in serum samples, and 9 peaks with statistical differences. Among them, 9 peaks with M/Z values of 3382.59, 3453.39, 4608.28, 5825.48, 8936.76, 9164.27, 11 525.30, 11 689.40 and 11 886.00 were significantly up-regulated (P < 0.05 or P < 0.01), compared with control group.
  
  
  CONCLUSIONS
  The present work demonstrated that SELDI-TOF-MS technology be capable of detecting differentially expressed protein peaks in BALF and serum samples from IPF patients. Further investigations were guaranteed to elucidate their potential diagnostic value in clinical practice.
authors: Department of Respirology and Critical Care Medicine, Logistics University of Chinese People's Armed Police Forces, Tianjin, China. luqing-wei@163.com
authors: Lu-qing
authors: LQ
authors: Wei
authors: Xue-ren
authors: XR
authors: Li
authors: Bin
authors: B
authors: Liu
authors: Shou-chun
authors: SC
authors: Peng
mesh_terms: D000328:Adult; D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D054988:Idiopathic Interstitial Pneumonias / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010449:Peptide Mapping; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D044967:Serum / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D055815:Young Adult
chemical_list: D011506:Proteins