title: IgE reactivity to a Cry j 3, an allergen of Japanese cedar (Cryptomeria japonica) pollen in dogs with canine atopic dermatitis.
abstract: The present study investigated IgE reactivity to a new Cryptomeria japonica pollen allergen (Cry j 3) in dogs with atopic dermatitis by using a fluorometric ELISA. Serum samples from 15 dogs that showed IgE sensitivity to crude C. japonica pollen allergen by ELISA were tested for specific IgE to each allergen, individually. All 15 dogs had anti-Cry j 1 IgE, 6 (40%) had anti-Cry j 2 IgE, and 11 (73%) had anti-Cry j 3 IgE. Further, we found that these anti-Cry j 3 IgE reacted to Cry j 3 with immunoblotting analysis. These findings indicate that Cry j 3 may be a major allergen in dogs.
authors: Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
authors: Shota
authors: S
authors: Kubota
authors: Kazuki
authors: K
authors: Miyaji
authors: Yusaku
authors: Y
authors: Shimo
authors: Hidekatsu
authors: H
authors: Shimakura
authors: Yukari
authors: Y
authors: Takase
authors: Noriaki
authors: N
authors: Okamoto
authors: Akio
authors: A
authors: Kiuchi
authors: Masato
authors: M
authors: Fujimura
authors: Takashi
authors: T
authors: Fujimura
authors: Douglas J
authors: DJ
authors: DeBoer
authors: Toshihiro
authors: T
authors: Tsukui
authors: Masahiro
authors: M
authors: Sakaguchi
mesh_terms: D000818:Animals; D052179:Antigens, Plant / Q000276:immunology*; D032485:Cryptomeria / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D011058:Pollen / Q000276:immunology
chemical_list: D052179:Antigens, Plant; C518869:Cry j 3 allergen, Cryptomeria japonica; D007073:Immunoglobulin E