title: Revealing non-genetic adhesive variations in clonal populations by comparative single-cell force spectroscopy.
abstract: Cell populations often display heterogeneous behavior, including cell-to-cell variations in morphology, adhesion and spreading. However, better understanding the significance of such cell variations for the function of the population as a whole requires quantitative single-cell assays. To investigate adhesion variability in a CHO cell population in detail, we measured integrin-mediated adhesion to laminin and collagen, two ubiquitous ECM components, by AFM-based single-cell force spectroscopy (SCFS). CHO cells generally adhered more strongly to laminin than collagen but population adhesion force distributions to both ECM components were broad and partially overlapped. To determine the levels of laminin and collagen binding in individual cells directly, we alternatingly measured single cells on adjacent microstripes of collagen and laminin arrayed on the same adhesion substrate. In repeated measurements (≥60) individual cells showed a stable and ECM type-specific adhesion response. All tested cells bound laminin more strongly, but the scale of laminin over collagen binding varied between cells. Together, this demonstrates that adhesion levels to different ECM components are tightly yet differently set in each cell of the population. Adhesion variability to laminin was non-genetic and cell cycle-independent but scaled with the range of α6 integrin expression on the cell surface. Adhesive cell-to-cell variations due to varying receptor expression levels thus appear to be an inherent feature of cell populations and should to be considered when fully characterizing population adhesion. In this approach, SCFS performed on multifunctional adhesion substrates can provide quantitative single-cell information not obtainable from population-averaging measurements on homogeneous adhesion substrates.
authors: Center for Functional Nanostructures, Karlsruher Institut für Technologie, Wolfgang-Gaede-Strasse 1a, 76131 Karlsruhe, Germany.
authors: Lu
authors: L
authors: Dao
authors: Ulrich
authors: U
authors: Weiland
authors: Mario
authors: M
authors: Hauser
authors: Irina
authors: I
authors: Nazarenko
authors: Heinz
authors: H
authors: Kalt
authors: Martin
authors: M
authors: Bastmeyer
authors: Clemens M
authors: CM
authors: Franz
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D005109:Extracellular Matrix / Q000378:metabolism* / Q000648:ultrastructure*; D005434:Flow Cytometry; D039503:Integrin alpha6 / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D018625:Microscopy, Atomic Force; D011485:Protein Binding
chemical_list: D039503:Integrin alpha6; D007797:Laminin; D003094:Collagen