title: Plasma and exhaled breath condensate nitrite-nitrate level in relation to environmental exposures in adults in the EGEA study.
abstract: This study evaluated the associations between biological markers in the nitrate-nitrite-NO pathway and four environmental exposures among subjects examined in the second survey (2003-2007) of the French Epidemiological study on Genetics and Environment of Asthma (EGEA). Total nitrite and nitrate (NO(2)(-) /NO(3)(-)) levels were measured both in plasma and in exhaled breath condensate (EBC) in 949 adults. Smoking, diet and exposure to chlorine products were assessed using standardized questionnaires. Exposure to air pollutants was estimated by using geostatistical models. All estimates were obtained with generalized estimating equations for linear regression models. Median levels of NO(2)(-)/NO(3)(-) were 36.3 μM (1st-3rd quartile: 25.7, 51.1) in plasma and 2.0 μmol/mg proteins (1st-3rd quartile 0.9, 3.9) in EBC. After adjustment for asthma, age, sex and menopausal status, plasma NO(2)(-)/NO(3)(-) level increased with leafy vegetable consumption (above versus below median=0.04 (95%CI: 0.001, 0.07)) and decreased in smokers (versus non/ex-smokers=-0.08 (95%CI: -0.11, -0.04). EBC NO(2)(-)/NO(3)(-) level decreased in smokers (-0.08 (95%CI: -0.16, -0.001)) and with exposure to ambient O(3) concentration (above versus below median=-0.10 (95%CI: -0.17, -0.03)). Cured meat, chlorine products, PM(10) and NO(2) concentrations were not associated with NO(2)(-)/NO(3)(-) levels. Results suggest that potential modifiable environmental and behavioral risk factors may modify NO(2)(-)/NO(3)(-) levels in plasma and EBC according to the route of exposure.
authors: Respiratory and Environmental Epidemiology, CESP Centre for Research in Epidemiology and Population Health, U1018, Inserm, 16, Avenue Paul Vaillant Couturier, F-94807 Villejuif, France. marta.rava@inserm.fr
authors: Marta
authors: M
authors: Rava
authors: Raphäelle
authors: R
authors: Varraso
authors: Brigitte
authors: B
authors: Decoster
authors: Hélène
authors: H
authors: Huyvaert
authors: Nicole
authors: N
authors: Le Moual
authors: Bénédicte
authors: B
authors: Jacquemin
authors: Nino
authors: N
authors: Künzli
authors: Francine
authors: F
authors: Kauffmann
authors: Farid
authors: F
authors: Zerimech
authors: Régis
authors: R
authors: Matran
authors: Rachel
authors: R
authors: Nadif
mesh_terms: D000293:Adolescent; D000328:Adult; D000393:Air Pollutants / Q000032:analysis* / Q000493:pharmacokinetics; D001249:Asthma / Q000097:blood / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests / Q000379:methods; D004032:Diet; D004781:Environmental Exposure / Q000032:analysis*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000097:blood / Q000378:metabolism*; D009573:Nitrites / Q000097:blood / Q000378:metabolism*; D012907:Smoking / Q000097:blood / Q000378:metabolism; D018709:Statistics, Nonparametric
chemical_list: D000393:Air Pollutants; D015415:Biomarkers; D009566:Nitrates; D009573:Nitrites