title: Porphyromonas gingivalis HmuY-induced production of interleukin-6 and IL-6 polymorphism in chronic periodontitis.
abstract: BACKGROUND
  In chronic periodontitis (CP), the gene polymorphism of interleukin-6 (IL-6) to 174C/G has been associated with the altered production of this cytokine. The aim of this pilot study is to compare the allelic and genotypic frequencies in patients with CP with control individuals without periodontitis (NP) and to measure the production of IL-6 by whole blood cells stimulated with Porphyromonas gingivalis HmuY protein.
  
  
  METHODS
  DNA was isolated from peripheral blood cells of 49 patients with CP and 60 control individuals classified as NP, and genotyping was performed by polymerase chain reaction using sequence-specific primers. Whole blood cells from 29 patients with CP and 30 control individuals were stimulated for 48 hours with HmuY, and IL-6 levels were measured using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The proportion of individuals carrying the G allele at position -174 of the IL-6 gene was higher in the group with CP (85.7%) than in the normal control group (73.3%; P <0.03). P. gingivalis HmuY-induced production of IL-6 was higher in the group with CP (P <0.05).
  
  
  CONCLUSIONS
  Our findings suggest that P. gingivalis HmuY may be associated with increased IL-6 production during CP. Furthermore, patients with periodontitis and individuals with higher HmuY-induced production of IL-6 show a high frequency of the G allele at position -174.
authors: Department of Immunology, Federal University of Bahia, Bahia, Brazil. soraya@uefs.br
authors: Soraya Castro
authors: SC
authors: Trindade
authors: Teresa
authors: T
authors: Olczak
authors: Isaac Suzart
authors: IS
authors: Gomes-Filho
authors: Lília Ferreira
authors: LF
authors: de Moura-Costa
authors: Vera Costa
authors: VC
authors: Vale
authors: Milton
authors: M
authors: Galdino-Neto
authors: Heidiane
authors: H
authors: Alves Dos Santos
authors: Paulo Cirino
authors: PC
authors: de Carvalho Filho
authors: Andreas
authors: A
authors: Stocker
authors: Maria Teresita
authors: MT
authors: Bendicho
authors: Márcia Tosta
authors: MT
authors: Xavier
authors: Eneida
authors: E
authors: de Moraes Marcílio Cerqueira
authors: Roberto
authors: R
authors: Meyer
mesh_terms: D000328:Adult; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology; D001426:Bacterial Proteins / Q000502:physiology*; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D005260:Female; D005787:Gene Frequency; D054884:Host-Pathogen Interactions; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011110:Polymorphism, Genetic; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000502:physiology; D012372:ROC Curve; D018709:Statistics, Nonparametric
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D015850:Interleukin-6