title: Alginate scaffolds for mesenchymal stem cell cardiac therapy: influence of alginate composition.
abstract: Despite the success of alginate scaffolds and mesenchymal stem cells (MSCs) therapy in cardiac failure treatment, the impact of the physicochemical environment provided by alginate matrices on cell behavior has never been investigated. The purpose of this work was double: to determine  the alginate composition influence on (1) encapsulated rat MSC viability, paracrine activity, and phenotype in vitro and (2) cardiac implantability and in vivo biocompatibility of patch shape scaffolds. Two alginates, differing in composition and thus presenting different mechanical properties  when hydrogels, were characterized. In both cases, encapsulated MSC viability was maintained at around 75%, and their secretion characteristics were retained 28 days postencapsulation. In vivo study revealed a high cardiac compatibility of the tested alginates: cardiac parameters were maintained,  and rats did not present any sign of infection. Moreover, explanted hydrogels appeared surrounded by a vascularized tissue. However, scaffold implantability was highly dependent on alginate composition. G-type alginate patches, presenting higher elastic and Young moduli than M-type alginate  patches, showed a better implantation easiness and were the only ones that maintained their shape and morphology in vivo. As a consequence of alginate chemical composition and resulting hydrogel structuration, G-type alginate hydrogels appear to be more adapted for cardiac implantation.
authors: Université de Toulouse, CIRIMAT, UPS-INPT-CNRS, Faculté de Pharmacie, Toulouse, France. ceccaldi@cict.fr
authors: Caroline
authors: C
authors: Ceccaldi
authors: Sophie Girod
authors: SG
authors: Fullana
authors: Chiara
authors: C
authors: Alfarano
authors: Olivier
authors: O
authors: Lairez
authors: Denis
authors: D
authors: Calise
authors: Daniel
authors: D
authors: Cussac
authors: Angelo
authors: A
authors: Parini
authors: Brigitte
authors: B
authors: Sallerin
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D005260:Female; D006321:Heart / Q000187:drug effects / Q000502:physiology* / Q000503:physiopathology; D006333:Heart Failure / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D020100:Hydrogels / Q000737:chemistry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D020100:Hydrogels