title: Comparative effects of levamisole, Staphylococcus, and Freund's adjuvant on rat immunization with excretory and secretory antigens of Trichinella spiralis muscle larvae.
abstract: A comparison was made of the effects of levamisole, the bacterial fractions of Staphylococcus, and Freund's adjuvant on the immunization of rats with the excretory and secretory antigens of Trichinella spiralis muscle larvae. Wistar rats were immunized with the antigen and a saline solution, levamisole (LV), Staphylococcus (ST), or Freund's adjuvant (FA). After immunization, rats were infected, and the parasite burden at muscular phase was calculated for each group. Levels of IgG1 and IgG2 antibodies, as well as levels of two cytokines, IL-4 and IFN-γ, were evaluated during the immunization and postinfection periods. Differences were found in the kinetics of antibody production between groups (p < 0.01). In all cases, there was reactivity with the main 45-, 50-, and 55-kDa antigens of Trichinella muscle larvae. Immunization with FA and ST enhanced the production of IgG1, but only FA showed a significant increase in the production of IFN-γ (p < 0.01), resulting in 86% protection against the infection. In contrast, only 60-70% protection was attained in the ST and LV groups (p < 0.01). These data support the idea that levamisole and Staphylococcus can be used as adjuvant to enhance the humoral response and, at the same time, demonstrate that IFN-γ could be involved in protection against Trichinella.
authors: Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaria de Salud, México D.F., Mexico 11340.
authors: Jorge-Luis
authors: JL
authors: de-la-Rosa-Arana
authors: Rafael
authors: R
authors: Campos-Rodríguez
authors: Víctor
authors: V
authors: Rivera-Aguilar
authors: Alejandro
authors: A
authors: Escobar-Gutiérrez
authors: Ángel
authors: Á
authors: Miliar-García
authors: Norma-Elena
authors: NE
authors: Herrera-González
authors: Rosa-Adriana
authors: RA
authors: Jarillo-Luna
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005620:Freund's Adjuvant / Q000008:administration & dosage*; D007114:Immunization / Q000379:methods*; D007978:Levamisole / Q000008:administration & dosage*; D059208:Parasite Load; D051381:Rats; D017208:Rats, Wistar; D013210:Staphylococcus / Q000276:immunology; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control
chemical_list: D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; D007978:Levamisole; D005620:Freund's Adjuvant