title: Gustatory perception and fat body energy metabolism are jointly affected by vitellogenin and juvenile hormone in honey bees.
abstract: Honey bees (Apis mellifera) provide a system for studying social and food-related behavior. A caste of workers performs age-related tasks: young bees (nurses) usually feed the brood and other adult bees inside the nest, while older bees (foragers) forage outside for pollen, a protein/lipid source, or nectar, a carbohydrate source. The workers' transition from nursing to foraging and their foraging preferences correlate with differences in gustatory perception, metabolic gene expression, and endocrine physiology including the endocrine factors vitellogenin (Vg) and juvenile hormone (JH). However, the understanding of connections among social behavior, energy metabolism, and endocrine factors is incomplete. We used RNA interference (RNAi) to perturb the gene network of Vg and JH to learn more about these connections through effects on gustation, gene transcripts, and physiology. The RNAi perturbation was achieved by single and double knockdown of the genes ultraspiracle (usp) and vg, which encode a putative JH receptor and Vg, respectively. The double knockdown enhanced gustatory perception and elevated hemolymph glucose, trehalose, and JH. We also observed transcriptional responses in insulin like peptide 1 (ilp1), the adipokinetic hormone receptor (AKHR), and cGMP-dependent protein kinase (PKG, or "foraging gene" Amfor). Our study demonstrates that the Vg-JH regulatory module controls changes in carbohydrate metabolism, but not lipid metabolism, when worker bees shift from nursing to foraging. The module is also placed upstream of ilp1, AKHR, and PKG for the first time. As insulin, adipokinetic hormone (AKH), and PKG pathways influence metabolism and gustation in many animals, we propose that honey bees have conserved pathways in carbohydrate metabolism and conserved connections between energy metabolism and gustatory perception. Thus, perhaps the bee can make general contributions to the understanding of food-related behavior and metabolic disorders.
authors: School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America. Ying.Wang.6@asu.edu
authors: Ying
authors: Y
authors: Wang
authors: Colin S
authors: CS
authors: Brent
authors: Erin
authors: E
authors: Fennern
authors: Gro V
authors: GV
authors: Amdam
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism / Q000503:physiopathology; D000818:Animals; D001516:Bees / Q000235:genetics* / Q000502:physiology; D050260:Carbohydrate Metabolism / Q000235:genetics / Q000502:physiology; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism; D004734:Energy Metabolism; D005216:Fat Body / Q000378:metabolism; D005247:Feeding Behavior / Q000502:physiology; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D007301:Insect Hormones / Q000378:metabolism; D007605:Juvenile Hormones / Q000235:genetics* / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D034741:RNA, Small Interfering; D055697:Taste Perception / Q000235:genetics / Q000502:physiology; D014819:Vitellogenins / Q000235:genetics* / Q000378:metabolism
chemical_list: D007301:Insect Hormones; D007605:Juvenile Hormones; D009842:Oligopeptides; D034741:RNA, Small Interfering; D014819:Vitellogenins; C044833:adipokinetic hormone; D017869:Cyclic GMP-Dependent Protein Kinases; D011761:Pyrrolidonecarboxylic Acid