title: The effect of the extract of Crocus sativus and its constituent safranal, on lung pathology and lung inflammation of ovalbumin sensitized guinea-pigs.
abstract: Different pharmacological effects of Crocus sativus have been demonstrated on guinea pig tracheal chains in previous studies. In the present study, the prophylactic effect of the extract of C. sativus and its constituent, safranal on lung pathology and total and differential white blood cells (WBC) of sensitized guinea pigs was examined. Guinea pigs were sensitized with injection and inhalation of ovalbumin (OA). One group of sensitized guinea pigs were given drinking water alone (group S) and three groups were given drinking water containing three concentrations of safranal (S+SA1, S+SA2 and S+SA3 groups), three groups, drinking water containing three concentrations of extract (S+CS1, S+CS2 and S+CS3 groups) and one group drinking water containing one concentration of dexamethasone (S+D group) (n=6, for all groups). The lung pathology was evaluated in control, non treated and treated sensitized groups. Total and differential WBC counts of lung lavage were also examined. All pathological indices in group S showed significant increased compared to control group (p<0.05 for lung congestion and p<0.001 for other groups). Total WBC number (p<0.001), eosinophyl percentage (p<0.001) in lung lavage and serum histamine levels (p<0.01) were also increased in sensitized animals compared to those of controls. Treatment of S animals with dexamethasone, all concentrations of the extract and safranal significantly improved lung pathological changes, most types of WBC and serum histamine levels compared to group S (p<0.05-0.001). Treatment of S group with first concentration of safranal also decreased total WBC. Treatment with safranal was more effective in improvement of most pathological changes, total and differential WBC count as well as serum histamine level (p<0.05-0.001). These results showed a preventive effect of the extract of C. sativus and its constituent safranal on lung inflammation of sensitized guinea pigs. The results also showed that the effect of the plant is perhaps due to its constituent safranal.
authors: Department of Physiology, School of Medicine and Pharmaceutical Research Centre, Mashhad University of Medical Sciences, Mashhad 177948564, Iran. mhboskabady@hotmail.com
authors: M H
authors: MH
authors: Boskabady
authors: A
authors: A
authors: Tabatabaee
authors: G
authors: G
authors: Byrami
mesh_terms: D000818:Animals; D027622:Crocus / Q000737:chemistry*; D053138:Cyclohexenes / Q000494:pharmacology / Q000627:therapeutic use*; D003907:Dexamethasone / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000097:blood; D007249:Inflammation / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D007958:Leukocyte Count; D007962:Leukocytes / Q000378:metabolism*; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008171:Lung Diseases / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D010047:Ovalbumin; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D013729:Terpenes / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D053138:Cyclohexenes; D010936:Plant Extracts; D013729:Terpenes; C087963:safranal; D003907:Dexamethasone; D006632:Histamine; D010047:Ovalbumin