title: Multicolor two-photon tissue imaging by wavelength mixing.
abstract: We achieve simultaneous two-photon excitation of three chromophores with distinct absorption spectra using synchronized pulses from a femtosecond laser and an optical parametric oscillator. The two beams generate separate multiphoton processes, and their spatiotemporal overlap provides an additional two-photon excitation route, with submicrometer overlay of the color channels. We report volume and live multicolor imaging of 'Brainbow'-labeled tissues as well as simultaneous three-color fluorescence and third-harmonic imaging of fly embryos.
authors: Laboratory for Optics and Biosciences, Ecole Polytechnique, Centre National de la Recherche Scientifique UMR 7645 and INSERM U696, Palaiseau, France.
authors: Pierre
authors: P
authors: Mahou
authors: Maxwell
authors: M
authors: Zimmerley
authors: Karine
authors: K
authors: Loulier
authors: Katherine S
authors: KS
authors: Matho
authors: Guillaume
authors: G
authors: Labroille
authors: Xavier
authors: X
authors: Morin
authors: Willy
authors: W
authors: Supatto
authors: Jean
authors: J
authors: Livet
authors: Delphine
authors: D
authors: Débarre
authors: Emmanuel
authors: E
authors: Beaurepaire
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology; D003116:Color; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology; D005453:Fluorescence; D007834:Lasers; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D017785:Photons; D013997:Time Factors