title: [Comparison of the mechanism of cardiotonic action of the fraction 3 of MAI-BAO and digoxin on rat isolated heart].
abstract: MAI-BAO (MB) is a beverage obtained by fermentation of sweet Camellia sinensis tea by the Tea-Mushroom treasure. The action of the chromatographic fraction 3 of MAI-BAO (MB-F3) is cardiotonic.
  
  
  OBJECTIVE
  To compare the mechanism of cardiotonic activity of MB-F3 and digoxin (Dx) in rats.
  
  
  METHODS
  MB-F3 obtained by gel chromatography on sephadex G-50 and digoxin are used to infuse the isolated rat heart with the device Langendhorff for the record of cardiac contractions. The biochemical mechanism of cardiac effect of MB-F3 and Dx was determined by measuring phosphorus by the method of Sumner.
  
  
  RESULTS
  MB-F3 has a significant positive inotropic effect (P<0.05) as Dx (P <0.01) with respective effective Doses 50% 1mg/ml and 10-6 mg/ml. Furthermore, MB-F3 exerts a competitive inhibition on the ATPase-Na+/K+ less than Dx (P<0.05).
  
  
  CONCLUSIONS
  MB-F3 exerted digitalis-like effect less than digoxin on the rat heart.
authors: Laboratoire de Pharmacodynamie-Biochimique, UFR Biosciences, Universite de Cocody, Abidjan, Cote d’Ivoire. fyapo@yahoo.fr
authors: Adou Francis
authors: AF
authors: Yapo
authors: Houphouet Felix
authors: HF
authors: Yapi
authors: Alain dit Phillipe
authors: Ad
authors: Bidie
authors: Kouakou Jean-Claud
authors: KJ
authors: Abo
authors: Dama Albert
authors: DA
authors: Otokore
authors: Yao Jacques
authors: YJ
authors: Datte
authors: Allico Joseph
authors: AJ
authors: Djaman
mesh_terms: D000818:Animals; D028241:Camellia sinensis; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004077:Digoxin / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D009200:Myocardial Contraction / Q000187:drug effects*; D051381:Rats; D013662:Tea
chemical_list: D002316:Cardiotonic Agents; D013662:Tea; D004077:Digoxin