title: Human periprostatic adipose tissue: its influence on prostate cancer cells.
abstract: OBJECTIVE
  Adipose microenvironment is involved in signaling pathways that influence prostate cancer (PCa) progression. However, the role of human periprostatic adipose tissue (PPAT) from patients with benign prostatic hyperplasia (BPH) has not been studied and compared to that of PPAT from PCa patients. The aim of this paper was to investigate the influence of factors derived from both PPATs on the behavior of androgen-dependent and castration resistant PCa cells.
  
  
  METHODS
  PPAT conditioned media (CM) were obtained from tissue samples from patients with clinically primary PCa (TPPAT) or BPH (BPPAT). Cell adhesion, proliferation, migration and metalloproteinase expression were evaluated following exposure of LNCaP (androgen dependent) and PC3 (androgen independent) prostate cancer cell lines to BPPAT or TPPAT CM.
  
  
  RESULTS
  Proliferation or motility of LNCaP or PC3 cells were not significantly affected by TPPAT or BPPAT CM. The number of LNCaP but not PC3 cells attached to components of TPPAT CM significantly decreased compared to cells attached to BPPAT CM. PPAT produced and released pro-MMP-9. Zymograms demonstrated that TPPAT CM induced a significant increase in pro-MMP-9 activity compared to BPPAT CM in LNCaP cells but not in PC3 cells.
  
  
  CONCLUSIONS
  We conclude that TPPAT released factors, such as pro-MMP-9, could induce the invasive capacity of LNCaP cells and speculate that PPAT derived factors could, in the early stages of prostate cancer, modulate disease progression.
authors: Instituto de Biología y Medicina Experimental (IBYME)-CONICET, Buenos Aires, Argentina.
authors: Paula Alejandra
authors: PA
authors: Sacca
authors: Virginia Pistone
authors: VP
authors: Creydt
authors: Hosoon
authors: H
authors: Choi
authors: Osvaldo Néstor
authors: ON
authors: Mazza
authors: Sabrina Johanna
authors: SJ
authors: Fletcher
authors: Valeria Beatriz Fernández
authors: VB
authors: Vallone
authors: Carlos
authors: C
authors: Scorticati
authors: Norma Alejandra
authors: NA
authors: Chasseing
authors: Juan Carlos
authors: JC
authors: Calvo
mesh_terms: D000368:Aged; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D017077:Culture Media, Conditioned; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008875:Middle Aged; D009376:Neoplasms, Hormone-Dependent / Q000473:pathology*; D011467:Prostate / Q000473:pathology*; D011470:Prostatic Hyperplasia / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D059016:Tumor Microenvironment
chemical_list: D017077:Culture Media, Conditioned; D020780:Matrix Metalloproteinase 9