title: Functional interaction of TRPV4 channel protein with annexin A2 in DRG.
abstract: BACKGROUND
  Transient receptor potential vanilloid 4 (TRPV4) is a Ca(2+)-permeable, non-selective cation channel that is involved in the transmission of pain signals mediated by dorsal root ganglion (DRG). Annexin A2 belongs to a class of membrane-binding proteins that  plays an important role in the regulation of ion channels. Nevertheless, little is known about the interaction between them in DRG. In this paper, we evaluated the functional interaction of TRPV4 with annexin A2 in DRG.
  
  
  METHODS
  We have used immunocytochemistry and co-immunoprecipitation  assays to investigate the interaction between annexin A2 and TRPV4 in DRG. The role of annexin A2 in the regulation of TRPV4 activity in DRG was further verified by measurement of intracellular free calcium concentrations ([Ca(2+)](i)) and substance P (SP) release.
  
  
  RESULTS
  First, annexin A2 was showed partial co-localization with TRPV4 in DRG neurons. Then, annexin A2 and TRPV4 were co-precipitated with each other in DRG lysates. Furthermore, the downregulation of annexin A2 using specific small interfering RNA significantly inhibited Ca(2+) influx  and SP mediated by TRPV4.
  
  
  CONCLUSIONS
  Our results provide evidence that annexin A2 is associated with TRPV4 and regulates TRPV4-mediated Ca(2+) influx and SP release in DRG neurons. The objective of this work is to determine the influence of annexin A2 on TRPV4 in DRG neurons,  which may be the basis for treatment of pain relief.
authors: Department of Physical Medicine and Rehabilitation, Qilu Hospital, Shandong University, Jinan, China.
authors: Liping
authors: L
authors: Ning
authors: Chuanwei
authors: C
authors: Wang
authors: Xinli
authors: X
authors: Ding
authors: Yang
authors: Y
authors: Zhang
authors: Xuping
authors: X
authors: Wang
authors: Shouwei
authors: S
authors: Yue
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017306:Annexin A2 / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D015536:Down-Regulation / Q000502:physiology; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013373:Substance P / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D050916:TRPV Cation Channels / Q000378:metabolism / Q000502:physiology*
chemical_list: D017306:Annexin A2; D050916:TRPV Cation Channels; C486577:Trpv4 protein, rat; D013373:Substance P; D002118:Calcium