title: [Change of calcium channel alpha-2-delta-1 subunit expression levels in trigeminal neuralgia rats].
abstract: OBJECTIVE
  To determine the expression levels of calcium channel alpha-2-delta-1 (Cavalpha2delta-1) subunit in trigeminal ganglia (TG) and trigeminal spinal subnucleus caudalis C1-C2 spinal cervical dorsal horn(Vc/C2) after infraorbital nerve (ION) ligation injury in relation to the development of neuropathic nociception.
  
  
  METHODS
  Twelve male SD rats were divided into two groups randomly, with 6 rats for each. In the ligation group, the ION was ligated by chromic gut loosely. In the sham operation group, the ION was exposed under same procedure but not ligated. The behavioral tests were conducted before 1 d and after 3, 6, 9, 12, 15 d with calibrated von Frey filaments. Cavalpha2delta-1 protein levels in TG and Vc/C2 were measured with Western blots 15 days after surgery.
  
  
  RESULTS
  Compared with the sham operation group, changes of allodynia to mechanical stimulation on the territory of ligated ION were found from the 9th to 15th day after operation in the ligation group (P<0.05). The expression levels of Cavalpha2delta-1 in TG and Vc/ C2 after ION ligation injury upregulated significantly (P<0.01).
  
  
  CONCLUSIONS
  The expression levels of Cavalpha2delta-1 in TG and Vc/C2 after ION ligation injury were upregulated and it may contribute to the pathogenesis of neuropathic nociception.
authors: Dept. of Anesthesiology, School of Stomatology, China Medical University, Shenyang 110002, China.
authors: Xia
authors: X
authors: Zhang
authors: Xiaofeng
authors: X
authors: Bai
authors: Qian
authors: Q
authors: Zhang
mesh_terms: D000818:Animals; D002118:Calcium; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014277:Trigeminal Neuralgia
chemical_list: C486060:Cacna2d1 protein, rat; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D002118:Calcium