title: Odontoblast-like MDPC-23 cells function as odontoclasts with RANKL/M-CSF induction.
abstract: The identity of odontoclast precursors and the molecular mechanism by which odontoclasts resorb dentine remain unclear. MDPC-23 cells were derived from dental papilla cells and possessed odontoblast characteristics such as expressing dentine sialophosphoprotein (Dspp) and dentine matrix acidic phosphoprotein 1 (Dmp1). Here we induced MDPC-23 cells to have an odontoclast-like function with receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). We found that tartrate-resistant acid phosphatase (TRAP)-positive cells, TRAP-positive multinucleated cells on the dentine slice were significantly increased in RANKL/M-CSF-induced cells. Osteoclast-specific genes such as Trap, osteopetrosis-associated transmembrane protein 1 (Ostm1), chloride channel 7 (Clcn7), cathepsin K (Ctsk) as well as osteoclast-specific transcription factor and microphthalmia transcription factor (MITF) were up-regulated in the treated cells, whilst the messenger RNA (mRNA) levels of Dspp, Dmp1 and Opg were reduced in the induced cells. The intracellular environment became more acidic in the RANKL/M-CSF treatment group, which resulted in more absorptive pits in dentine slices. We suggested that RANKL/M-CSF might induce odontoblast-like MDPC-23 cells to differentiate into odontoclast-like cells or function as odontoclasts. Our data might provide a new explanation for the precursors of odontoclast and root resorption.
authors: Department of Oral Biology, School of Stomatology, The Fourth Military Medical University, 145 Changle West Road, Xi'an, Shaanxi 710032, PR China. xhduan@fmmu.edu.cn
authors: Xiaohong
authors: X
authors: Duan
authors: Ting
authors: T
authors: Yang
authors: Yanli
authors: Y
authors: Zhang
authors: Xuan
authors: X
authors: Wen
authors: Yang
authors: Y
authors: Xue
authors: Mi
authors: M
authors: Zhou
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D056657:Cathepsin K / Q000032:analysis; D002417:Cattle; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D048430:Cell Shape; D018118:Chloride Channels / Q000032:analysis; D003804:Dentin / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000032:analysis; D018257:Helix-Loop-Helix Motifs; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000032:analysis; D016350:Leucine Zippers; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000032:analysis; D009804:Odontoblasts / Q000187:drug effects*; D010010:Osteoclasts / Q000166:cytology / Q000502:physiology*; D010750:Phosphoproteins / Q000032:analysis; D053245:RANK Ligand / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D012391:Root Resorption / Q000473:pathology; D012795:Sialoglycoproteins / Q000032:analysis; D000071681:Tartrate-Resistant Acid Phosphatase; D014157:Transcription Factors / Q000032:analysis; D015854:Up-Regulation; D000099311:Dentin Sialophosphoprotein
chemical_list: D000135:Acid Phosphatase; D056657:Cathepsin K; D018118:Chloride Channels; D016326:Extracellular Matrix Proteins; D007527:Isoenzymes; D016173:Macrophage Colony-Stimulating Factor; D008565:Membrane Proteins; D051739:Microphthalmia-Associated Transcription Factor; D010750:Phosphoproteins; D053245:RANK Ligand; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D000071681:Tartrate-Resistant Acid Phosphatase; D014157:Transcription Factors; D000099311:Dentin Sialophosphoprotein; C490803:Clcn7 protein, mouse; C513308:Dmp1 protein, mouse; C497235:Mitf protein, mouse; C509143:OSTM1 protein, mouse; C505689:Tnfsf11 protein, mouse; C000605511:Acp5 protein, mouse; C534132:Ctsk protein, mouse