title: Members 5 and 6 of the Candida albicans BMT family encode enzymes acting specifically on β-mannosylation of the phospholipomannan cell-wall glycosphingolipid.
abstract: A family of nine genes encoding proteins involved in the synthesis of β-1,2 mannose adhesins of Candida albicans has been identified. Four of these genes, BMT1-4, encode enzymes acting stepwise to add β-mannoses on to cell-wall phosphopeptidomannan (PPM). None of these acts on phospholipomannan (PLM), a glycosphingolipid member of the mannose-inositol-phosphoceramide family, which contributes with PPM to β-mannose surface expression. We show that deletion of BMT5 and BMT6 led to a dramatic reduction of PLM glycosylation and accumulation of PLM with a truncated β-oligomannoside chain, respectively. Disruptions had no effect on sphingolipid biosynthesis and on PPM β-mannosylation. β-Mannose surface expression was not affected, confirming that β-mannosylation is a process based on specificity of acceptor molecules, but liable to global regulation.
authors: Unité INSERM, Lille, France.
authors: Céline
authors: C
authors: Mille
authors: Chantal
authors: C
authors: Fradin
authors: Florence
authors: F
authors: Delplace
authors: Pierre-André
authors: PA
authors: Trinel
authors: Annick
authors: A
authors: Masset
authors: Nadine
authors: N
authors: François
authors: Bernadette
authors: B
authors: Coddeville
authors: Piotr
authors: P
authors: Bobrowicz
authors: Thierry
authors: T
authors: Jouault
authors: Yann
authors: Y
authors: Guerardel
authors: Stefan
authors: S
authors: Wildt
authors: Guilhem
authors: G
authors: Janbon
authors: Daniel
authors: D
authors: Poulain
mesh_terms: D000123:Acetyltransferases; D001426:Bacterial Proteins; D002176:Candida albicans / Q000201:enzymology*; D002473:Cell Wall / Q000737:chemistry*; D004789:Enzyme Activation; D006017:Glycolipids / Q000378:metabolism*; D006031:Glycosylation; D008351:Mannans / Q000378:metabolism*; D010748:Phosphopeptides / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D006017:Glycolipids; D008351:Mannans; D010748:Phosphopeptides; C400188:phospholipomannan; C072108:phosphopeptidomannan; D000123:Acetyltransferases; C094574:BltD protein, Bacillus subtilis