title: Nanoparticle-mediated gene transfer from electrophoretically coated metal surfaces.
abstract: The transfer of genetic information into living cells is a powerful tool to manipulate their protein expression by the regulation of protein synthesis. This can be used for the treatment of genetically caused diseases (gene therapy). However, the systemic application of genes is associated with a number of problems, such as a targeted gene delivery and potential side effects. Here we present a method for the spatial application of nanoparticle-based gene therapy. Titanium was electrophoretically coated with DNA-functionalized calcium phosphate nanoparticles. NIH3T3 cells and HeLa cells were transfected with pcDNA3-EGFP. We monitored the transfection in vitro by fluorescence microscopy, flow cytometry, and Western Blot analysis. By coating a transparent substrate, i.e., indium tin oxide (ITO), with nanoparticles, we followed the transfection by live cell imaging.
authors: Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45117 Essen, Germany.
authors: Anna
authors: A
authors: Kovtun
authors: Sebastian
authors: S
authors: Neumann
authors: Manuel
authors: M
authors: Neumeier
authors: Henning
authors: H
authors: Urch
authors: Rolf
authors: R
authors: Heumann
authors: Michael M
authors: MM
authors: Gepp
authors: Katrin
authors: K
authors: Wallat
authors: Manfred
authors: M
authors: Koeller
authors: Heiko
authors: H
authors: Zimmermann
authors: Matthias
authors: M
authors: Epple
mesh_terms: D000818:Animals; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008670:Metals / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry; D014162:Transfection
chemical_list: D008670:Metals; D017971:Tin Compounds; D049452:Green Fluorescent Proteins; C109984:indium tin oxide