title: Methylation status of NEUROG2 and NID2 improves the diagnosis of stage I NSCLC.
abstract: In our previous study, we attempted to develop a tool for the early diagnosis of non-small cell lung cancer (NSCLC) using DNA methylation biomarkers. With the aim of improving the diagnostic potential by optimizing the composition of the target set, in this study, 13 candidate genes (ACTA1, AIDH1A2, CBX8, CDH8, EVX1, MGC16275, NEUROG1, NEUROG2, NID2, OTX2OS1, PGAM2, PHOX2B and TOX) were analyzed by methylation-specific PCR to determine the methylation status of each gene in 5 NSCLC cell lines and in lung tissue samples from 15 healthy volunteers, 103 stage I NSCLC patients and 26 non-cancerous control patients. Results showed that NEUROG2 and NID2 were hypermethylated in stage I NSCLC tissues (31.07 and 46.60%, respectively) and unmethylated in normal lung tissues (0/15) and non-cancerous tissues (0/26). Following recombination, an optimized 5-gene panel (NEUROG2, NID2, RASSF1A, APC and HOXC9) achieved a sensitivity of 91.26% with a specificity of 84.62% in the detection of stage I NSCLC. The optimized 5-gene panel greatly improved the diagnostic power for stage I NSCLC.
authors: Department of General Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
authors: Junfeng
authors: J
authors: Geng
authors: Jinfeng
authors: J
authors: Sun
authors: Qiang
authors: Q
authors: Lin
authors: Jun
authors: J
authors: Gu
authors: Yangxing
authors: Y
authors: Zhao
authors: Hongyu
authors: H
authors: Zhang
authors: Xu
authors: X
authors: Feng
authors: Yinghua
authors: Y
authors: He
authors: Wei
authors: W
authors: Wang
authors: Xiaoyu
authors: X
authors: Zhou
authors: Jian
authors: J
authors: Yu