title: Tracking mesenchymal stem cells with iron oxide nanoparticle loaded poly(lactide-co-glycolide) microparticles.
abstract: Monitoring the location, distribution and long-term engraftment of administered cells is critical for demonstrating the success of a cell therapy. Among available imaging-based cell tracking tools, magnetic resonance imaging (MRI) is advantageous due to its noninvasiveness, deep penetration, and high spatial resolution. While tracking cells in preclinical models via internalized MRI contrast agents (iron oxide nanoparticles, IO-NPs) is a widely used method, IO-NPs suffer from low iron content per particle, low uptake in nonphagocytotic cell types (e.g., mesenchymal stem cells, MSCs), weak negative contrast, and decreased MRI signal due to cell proliferation and cellular exocytosis. Herein, we demonstrate that internalization of IO-NP (10 nm) loaded biodegradable poly(lactide-co-glycolide) microparticles (IO/PLGA-MPs, 0.4-3 μm) in MSCs enhances MR parameters such as the r(2) relaxivity (5-fold), residence time inside the cells (3-fold) and R(2) signal (2-fold) compared to IO-NPs alone. Intriguingly, in vitro and in vivo experiments demonstrate that internalization of IO/PLGA-MPs in MSCs does not compromise inherent cell properties such as viability, proliferation, migration and their ability to home to sites of inflammation.
authors: Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT, Division of Health Sciences and Technology, 65 Landsdowne Street, Cambridge, Massachusetts 02139, USA.
authors: Chenjie
authors: C
authors: Xu
authors: David
authors: D
authors: Miranda-Nieves
authors: James A
authors: JA
authors: Ankrum
authors: Mads Emil
authors: ME
authors: Matthiesen
authors: Joseph A
authors: JA
authors: Phillips
authors: Isaac
authors: I
authors: Roes
authors: Gregory R
authors: GR
authors: Wojtkiewicz
authors: Vikram
authors: V
authors: Juneja
authors: Jens Roat
authors: JR
authors: Kultima
authors: Weian
authors: W
authors: Zhao
authors: Praveen Kumar
authors: PK
authors: Vemula
authors: Charles P
authors: CP
authors: Lin
authors: Matthias
authors: M
authors: Nahrendorf
authors: Jeffrey M
authors: JM
authors: Karp
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011098:Polyglactin 910 / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D011098:Polyglactin 910