title: PinX1 is involved in telomerase recruitment and regulates telomerase function by mediating its localization.
abstract: Telomerase recruitment to telomere is the prerequisite for telomere extension, but the proteins involved in this process are still largely unknown. PinX1 is a telomerase inhibitor and has been implicated in telomere maintenance. Silencing of PinX1 significantly reduced the localization of telomerase to telomere during mid-late S phase, suggesting the involvement of PinX1 in the cell cycle-dependent trafficking of hTERT to telomere. We also revealed that PinX1 mediated the chromosomal localization of hTERT during anaphase. This study revealed the role of PinX1 in telomerase function regulation by mediating its localization inside cells.
authors: Biochemistry Programme, School of Life Sciences and Centre for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
authors: Derek Hang-Cheong
authors: DH
authors: Cheung
authors: Hsiang-Fu
authors: HF
authors: Kung
authors: Jun-Jian
authors: JJ
authors: Huang
authors: Pang-Chui
authors: PC
authors: Shaw
mesh_terms: D002453:Cell Cycle; D018797:Cell Cycle Proteins; D020868:Gene Silencing; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics; D016196:S Phase; D015398:Signal Transduction; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism; D035341:Telomeric Repeat Binding Protein 2 / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; C440808:PINX1 protein, human; D034741:RNA, Small Interfering; C504753:TERF2 protein, human; D035341:Telomeric Repeat Binding Protein 2; D025521:Tumor Suppressor Proteins; D019098:Telomerase