title: [Establishment of minireplicon system for severe fever with thrombocytopenia syndrome bunyavirus].
abstract: Severe fever with thrombocytopenia syndrome bunyavirus is a newly emerging virus in China, enveloped with a tripartite, single-stranded RNA genome of negative polarity. The regulatory elements for viral transcription and replication, as well as encapsidation and packaging signals, are thought to be located within these noncoding regions (NCRs). The terminal nucleotides are genus specific and highly conserved. The function of the remaining nucleotides of the NCRs is still not well understood. In this study, we developed the plasmid-driven RNA polymerase I minireplicon system for SFTSV firstly, using reporter genes GFP and luciferase. The function of the noncoding regions of the three Bunyaviridae RNA segments (L, M, S) in transcription was analyzed. Reporter genes are successfully expressed in SFTSV minireplicon system. Our results suggest that the NCRs of SFTSV from all three segments contain the necessary signals to initiate transcription. Quantitative detection of the luciferase expression level shows that promoter activity in the three segments is different.
authors: National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China. yu_8605@sina.com
authors: Xia-Li
authors: XL
authors: Yu
authors: Xiao-Lin
authors: XL
authors: Jiang
authors: Tao
authors: T
authors: Wang
authors: Yu-Lan
authors: YL
authors: Sun
authors: Shuo
authors: S
authors: Zhang
authors: Chuan
authors: C
authors: Li
authors: Quan-Fu
authors: QF
authors: Zhang
authors: Mi-Fang
authors: MF
authors: Liang
authors: Zhen-Qiang
authors: ZQ
authors: Bi
authors: De-Xin
authors: DX
authors: Li
mesh_terms: D002044:Bunyaviridae Infections / Q000821:virology*; D003001:Cloning, Molecular; D016679:Genome, Viral; D006801:Humans; D016856:Phlebovirus / Q000235:genetics* / Q000502:physiology; D012093:Replicon; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D014764:Viral Proteins