title: Versatile strategy for biochemical, electrochemical and immunoarray detection of protein phosphorylations.
abstract: Protein kinases catalyze the phosphorylation of cellular proteins involved in the regulation of many cellular processes and have emerged as promising targets for the treatment of several diseases. Conventional assays to monitor protein kinase activity are limited because they typically rely on transfer of radioactive phosphate or phospho-specific antibodies that recognize specific substrates or sequence motifs. To overcome the limitations of conventional assays, we have developed a versatile approach based on transfer of ferrocene-phosphate that can be readily monitored using electrochemical detection or detection with antiferrocene antibodies in an immunoarray format. This assay is readily adapted to multiplex arrays and can be employed for monitoring kinase activity in complex mixtures and for kinase inhibitor profiling.
authors: Department of Physical and Environmental Sciences, University of Toronto Scarborough, Toronto, Ontario M1C 1A4, Canada.
authors: Sanela
authors: S
authors: Martić
authors: Michelle
authors: M
authors: Gabriel
authors: Jacob P
authors: JP
authors: Turowec
authors: David W
authors: DW
authors: Litchfield
authors: Heinz-Bernhard
authors: HB
authors: Kraatz
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D055162:Biocatalysis; D055664:Electrochemical Techniques; D007118:Immunoassay; D010766:Phosphorylation; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits
chemical_list: D000906:Antibodies; D011494:Protein Kinases