title: Nanocomposites consisting of titanium dioxide nanoparticles and oligonucleotides.
abstract: The use of various nanoparticles is a promising way to solve the current problem of drug delivery in medicine and biology. Nanocomposites consisting of titanium dioxide and oligonucleotides noncovalently attached to nanoparticles through the polylysine linker (TiO2 x PL-DNA) have been designed to deliver of DNA fragments into cells. Three forms of TiO2 nanoparticles (amorphous, anatase, and brookite) were used for construction of nanocomposites. The size, morphology, and chemical composition of TiO2 nanoparticles and TiO2 x PL-DNA nanocomposites were characterized. DNA fragments in the proposed nanocomposites were shown to retain their ability to form complementary complexes. TiO2 x PL-DNA nanocomposites independently on the form of nanoparticles were shown by confocal microscopy to penetrate into HeLa cells without any transfection agents and physical impact. The presented type of nanocomposites can be applied in the thriving technology of drug delivery to achieve high therapeutic and biological efficacy.
authors: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of RAS, pr. Lavrent'eva 8, Novosibirsk 630090, Russia.
authors: Asya
authors: A
authors: Levina
authors: Zinfer
authors: Z
authors: Ismagilov
authors: Marina
authors: M
authors: Repkova
authors: Natalia
authors: N
authors: Shatskaya
authors: Nadezhda
authors: N
authors: Shikina
authors: Fedor
authors: F
authors: Tusikov
authors: Valentina
authors: V
authors: Zarytova
mesh_terms: D000818:Animals; D001483:Base Sequence; D006367:HeLa Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites; D009841:Oligonucleotides / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; C009495:titanium dioxide; D014025:Titanium