title: Antioxidant activity of different parts from Annona squamosa, and Catunaregam nilotica methanolic extract.
abstract: BACKGROUND
  We extracted phenolic compounds from Annona squamosa (leaves, bark, roots and seedcake), and Catunaregam nilotica (leaves, bark and seedcake) using methanol and their antioxidant activity was evaluated employing various established in vitro systems.
  
  
  METHODS
  Annona squamosa (leaves, bark, roots and seedcake), and Catunaregam nilotica (leaves, bark and seedcake) were used in the study. Antioxidant activity was estimated using oxygen radical absorbance capacity, MTT assay and DPPH assays, and polyphenols profile was determined by HPLC method.
  
  
  RESULTS
  The total phenolic content was determined by Folin-Ciocalteu method and the highest amounts were 171.5, 170.4, 169.5, and 167.9 g/kg plant extract as GAE for A. squamosa roots, C. nilotica bark, C. nilotica leaves, and A. squamosa bark, respectively. The leaves extracts of the two trees showed high flavoniod content. The results showed that C. nilotica and A. squamosa extracts displayed antioxidant activities, with IC(50) values ranging from 7.81 to 62.5 and from 7.81 to 125.0 μg/ml, respectively using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The different parts extracts from two trees showed good antioxidant activity evaluated by oxygen radical absorbance capacity and MTT assay systems.
  
  
  CONCLUSIONS
  These results suggested that Annona squamosa and Catunaregam nilotica phenolic compounds could be utilized as a natural antioxidant.
authors: Food Science and Technology Department, College of Agricultural Studies, Sudan University of Science and Technology P.O. Box 71 Khartoum North, Sudan. basitmariod@yahoo.com
authors: Abdalbasit Adam
authors: AA
authors: Mariod
authors: Siddig Ibrahim
authors: SI
authors: Abdelwahab
authors: Sara
authors: S
authors: Elkheir
authors: Yousif Mohamed
authors: YM
authors: Ahmed
authors: Putri Narrima Mohd
authors: PN
authors: Fauzi
authors: Cheah Shiau
authors: CS
authors: Chuen
mesh_terms: D000818:Animals; D029061:Annonaceae / Q000737:chemistry*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D010636:Phenols / Q000032:analysis; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D019666:Rubiaceae / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts