title: Ca2+ imaging in perfused adrenal medullae.
abstract: The Ca2+ imaging method was developed to explore changes in excitability in adrenal medullary (AM) cells in a large field in response to synaptic input and chemicals. The adrenal medullae of rats and guinea pigs were retrogradely loaded with Ca2+ indicator through the adrenal vein. Nerve fibers remaining in the adrenal gland were electrically stimulated to induce postsynaptic responses in AM cells, and chemicals were applied to the cells by adding to the perfusate. With this method, gamma-aminobutyric acid (GABA) was shown to increase the Ca2+ signal in almost all and 40% AM cells in guinea pigs and rats, respectively.
authors: Department of Cell and Systems Physiology, School of Medicine, University of Occupational and Environmental Health, Japan, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
authors: Akira
authors: A
authors: Warashina
authors: Masumi
authors: M
authors: Inoue
mesh_terms: D000313:Adrenal Medulla / Q000187:drug effects / Q000294:innervation*; D000818:Animals; D002118:Calcium; D004558:Electric Stimulation; D006168:Guinea Pigs; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D005680:gamma-Aminobutyric Acid; D002118:Calcium