title: Effects of grape seed polyphenols on oxidative damage in liver tissue of acutely and chronically exercised rats.
abstract: The objective of the present study was to investigate the effects of grape seed extract (GSE) supplementation on oxidative stress and antioxidant defense markers in liver tissue of acutely and chronically exercised rats. Rats were randomly assigned to six groups: Control (C), Control Chronic Exercise (CE), Control Acute Exercise (AE), GSE-supplemented Control (GC), GSE-supplemented Chronic Exercise(GCE) and GSE-supplemented Acute Exercise (GAE). Rats in the chronic exercise groups were subjected to a six-week treadmill running and in the acute exercise groups performed an exhaustive running. Rats in the GSE supplemented groups received GSE (100 mg.kg(-1) .day(-1) ) in drinking water for 6 weeks. Liver tissues of the rats were taken for the analysis of malondialdehyde (MDA), nitric oxide (NO) levels and total antioxidant activity (AOA) and xanthine oxidase (XO) activities. MDA levels decreased with GSE supplementation in control groups but increased in acute and chronic exercise groups compared to their non-supplemented control. NO levels increased with GSE supplementation. XO activities were higher in AE group compared to the CE group. AOA decreased with GSE supplementation. In conclusion, while acute exercise triggers oxidative stress, chronic exercise has protective role against oxidative stress. GSE has a limited antioxidant effect on exercise-induced oxidative stress in liver tissue.
authors: Department of Physiology, Meram Faculty of Medicine, Selçuk University, Konya, Turkey. mbelviranli@yahoo.com
authors: Muaz
authors: M
authors: Belviranlı
authors: Hakkı
authors: H
authors: Gökbel
authors: Nilsel
authors: N
authors: Okudan
authors: Sadık
authors: S
authors: Büyükbaş
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D056604:Grape Seed Extract / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009569:Nitric Oxide / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects*; D010805:Physical Conditioning, Animal / Q000009:adverse effects*; D059808:Polyphenols / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D056604:Grape Seed Extract; D059808:Polyphenols; D009569:Nitric Oxide; D008315:Malondialdehyde; D014969:Xanthine Oxidase