title: Dopaminergic modulation of the hippocampal neuropil proteome identified by bioorthogonal noncanonical amino acid tagging (BONCAT).
abstract: Local protein synthesis and its activity-dependent modulation via dopamine receptor stimulation play an important role in synaptic plasticity - allowing synapses to respond dynamically to changes in their activity patterns. We describe here the metabolic labeling, enrichment, and MS-based identification of candidate proteins specifically translated in intact hippocampal neuropil sections upon treatment with the selective D1/D5 receptor agonist SKF81297. Using the noncanonical amino acid azidohomoalanine and click chemistry, we identified over 300 newly synthesized proteins specific to dendrites and axons. Candidates specific for the SKF81297-treated samples were predominantly involved in protein synthesis and synapse-specific functions. Furthermore, we demonstrate a dendrite-specific increase in proteins synthesis upon application of SKF81297. This study provides the first snapshot in the dynamics of the dopaminergic hippocampal neuropil proteome.
authors: Division of Biology, California Institute of Technology, Pasadena, CA, USA.
authors: Jennifer J L
authors: JJ
authors: Hodas
authors: Anne
authors: A
authors: Nehring
authors: Nicole
authors: N
authors: Höche
authors: Michael J
authors: MJ
authors: Sweredoski
authors: Rainer
authors: R
authors: Pielot
authors: Sonja
authors: S
authors: Hess
authors: David A
authors: DA
authors: Tirrell
authors: Daniela C
authors: DC
authors: Dieterich
authors: Erin M
authors: EM
authors: Schuman
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D015153:Blotting, Western; D003712:Dendrites / Q000187:drug effects / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D018491:Dopamine Agonists / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D042282:Microdissection; D019581:Neuropil / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000596:Amino Acids; D001552:Benzazepines; D018491:Dopamine Agonists; D020543:Proteome; C067113:SK&F 81297; D004298:Dopamine