title: Two- and three-locus haplotypes of the paraoxonase (PON1) gene are associated with coronary artery disease in Asian Indians.
abstract: BACKGROUND
  In view of the reported association of SNPs in the paraoxonase (PON1) gene with coronary artery disease (CAD), and the absence of conclusive data from India, we investigated the relationship of three SNPs at different loci (-108C/T, L55M and Q192R) of the PON1 gene and their haplotypes with CAD among people residing in the northern plains of India.
  
  
  METHODS
  One hundred and seventy-eight healthy controls and two hundred and four angiographically-proven CAD patients were genotyped using PCR-RFLP.
  
  
  RESULTS
  Of the three SNPs, only the R allele of Q192R polymorphism was associated with CAD (p<0.05). Two locus haplotypes QT (OR 0.55, p=0.0004, 95% CI 0.39-0.77, significant) and LQ (odds ratio 0.73, p=0.03, 95% CI 0.55-0.97, trend) showed protective effects, while haplotypes MR (OR=5.36, p=0.0001, 95% CI 2.045-14.049) and MC (OR=2.71, p=0.011, 95% CI 1.221-6.046) were associated with increased risk of CAD. MRT, a minor three-locus haplotype also displayed significant association (OR 4.93, 95% CI 1.7-13.5) with the disease. Significance was assessed after applying Bonferroni's correction.
  
  
  CONCLUSIONS
  Our study revealed that only one SNP at a single locus but several haplotype combinations of PON1 coding and promoter-region polymorphisms were associated with the risk of or protection against CAD. Thus, haplotype analysis brought better insights into the association of PON1 gene polymorphisms with CAD in Asian Indians.
authors: Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029, India. imteyaz77@gmail.com
authors: Imteyaz
authors: I
authors: Ahmad
authors: Rajiv
authors: R
authors: Narang
authors: Anand
authors: A
authors: Venkatraman
authors: Nibhriti
authors: N
authors: Das
mesh_terms: D000328:Adult; D043303:Aryldialkylphosphatase / Q000235:genetics*; D001483:Base Sequence; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000201:enzymology* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D056726:Genetic Association Studies; D006239:Haplotypes; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012307:Risk Factors
chemical_list: D017931:DNA Primers; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human