title: Adherent and non-adherent dendritic cells are equivalently qualified in GM-CSF, IL-4 and TNF-α culture system.
abstract: We compared the purities, phenotype, capabilities of antigen uptake and T lymphocytes stimulation abilities of adherent and non-adherent cells of human monocyte-derived dendritic cells (mono-DCs) in GM-CSF, IL-4 and TNF-α culture system. The results show that both the purities and the capabilities of antigen uptake of the adherent DCs are significantly higher than those of non-adherent ones. As for the expression levels of surface markers and the abilities of stimulating lymphocytes proliferation, our results also show that those of adherent DCs are a bit higher than those of non-adherent ones, although the differences are not significant.
authors: Institute of Human Reproduction and Stem Cell Engineering, Central South University, Changsha, PR China.
authors: Hui-Jun
authors: HJ
authors: Yi
authors: Guang-Xiu
authors: GX
authors: Lu
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology*; D006801:Humans; D015847:Interleukin-4 / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: C508598:IL4 protein, human; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor