title: Protective effects of taurine against oxidative stress in the heart of MsrA knockout mice.
abstract: Taurine has been shown to have potent anti-oxidant properties under various pathophysiological conditions. We reported previously a cellular dysfunction and mitochondrial damage in cardiac myocytes of methionine sulfoxide reductase A (MsrA) gene knockout mice (MsrA(-/-)). In the present study, we have explored the protective effects of taurine against oxidative stress in the heart of MsrA(-/-) mice with or without taurine treatment. Cardiac cell contractility and Ca(2+) dynamics were measured using cell-based assays and in vivo cardiac function was monitored using high-resolution echocardiography in the tested animals. Our data have shown that MsrA(-/-) mice exhibited a progressive cardiac dysfunction with a significant decrease of ejection fraction (EF) and fraction shortening (FS) at age of 8 months compared to the wild type controls at the same age. However, the dysfunction was corrected in MsrA(-/-) mice treated with taurine supplement in the diet for 5 months. We further investigated the cellular mechanism underlying the protective effect of taurine in the heart. Our data indicated that cardiac myocytes from MsrA(-/-) mice treated with taurine exhibited an improved cell contraction and could tolerate oxidative stress better. Furthermore, taurine treatment reduced significantly the protein oxidation levels in mitochondria of MsrA(-/-) hearts, suggesting an anti-oxidant effect of taurine in cardiac mitochondria. Our study demonstrates that long-term treatment of taurine as a diet supplement is beneficial to a heart that is vulnerable to environmental oxidative stresses.
authors: Division of Cardiology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China.
authors: G
authors: G
authors: Chen
authors: C
authors: C
authors: Nan
authors: Jie
authors: J
authors: Tian
authors: P
authors: P
authors: Jean-Charles
authors: Y
authors: Y
authors: Li
authors: H
authors: H
authors: Weissbach
authors: X P
authors: XP
authors: Huang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D002118:Calcium / Q000378:metabolism; D019587:Dietary Supplements; D004452:Echocardiography; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D006334:Heart Function Tests; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051150:Methionine Sulfoxide Reductases / Q000172:deficiency / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout; D008928:Mitochondria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D013654:Taurine / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D013654:Taurine; D006861:Hydrogen Peroxide; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase; D002118:Calcium