title: Additive manufacturing of wet-spun polymeric scaffolds for bone tissue engineering.
abstract: An Additive Manufacturing technique for the fabrication of three-dimensional polymeric scaffolds, based on wet-spinning of poly(ε-caprolactone) (PCL) or PCL/hydroxyapatite (HA) solutions, was developed. The processing conditions to fabricate scaffolds with a layer-by-layer approach were optimized by studying their influence on fibres morphology and alignment. Two different scaffold architectures were designed and fabricated by tuning inter-fibre distance and fibres staggering. The developed scaffolds showed good reproducibility of the internal architecture characterized by highly porous, aligned fibres with an average diameter in the range 200-250 μm. Mechanical characterization showed that the architecture and HA loading influenced the scaffold compressive modulus and strength. Cell culture experiments employing MC3T3-E1 preosteoblast cell line showed good cell adhesion, proliferation, alkaline phosphatase activity and bone mineralization on the developed scaffolds.
authors: Laboratory of Bioactive Polymeric Materials for Biomedical and Environmental Applications (BIOlab), Department of Chemistry and Industrial Chemistry, University of Pisa, via Vecchia Livornese 1291, San Piero a Grado (Pi), 56010, Pisa, Italy.
authors: Dario
authors: D
authors: Puppi
authors: Carlos
authors: C
authors: Mota
authors: Matteo
authors: M
authors: Gazzarri
authors: Dinuccio
authors: D
authors: Dinucci
authors: Antonio
authors: A
authors: Gloria
authors: Mairam
authors: M
authors: Myrzabekova
authors: Luigi
authors: L
authors: Ambrosio
authors: Federica
authors: F
authors: Chiellini
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000378:metabolism; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D017886:Durapatite / Q000378:metabolism; D004867:Equipment Design / Q000379:methods; D008422:Materials Testing; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D015203:Reproducibility of Results; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; C010078:alizarin; D017886:Durapatite; D000469:Alkaline Phosphatase