title: Analysis of the complete genome sequence of two Korean sacbrood viruses in the Honey bee, Apis mellifera.
abstract: The complete genomic RNAs of two Korean sacbrood virus (SBV) strains, which infect the honey bee, Apis mellifera, were sequenced. The two sequences (AmSBV-Kor19, AmSBV-Kor21) were distinguished by the presence or absence of a PstI restriction site. These strains comprised of 8784 bp and 8835 bp; contained a single large ORF (179-8707 and 179-8758) encoding 2843 and 2860 amino acids, respectively. Deduced amino acid sequences comparison with some insect viruses showed that regions of helicase, protease and RdRp domains; structural genes were located at the 5' end and non-structural genes at the 3' end. Multiple sequence alignment showed that AmSBV-Kor19 was missing a section between nucleotides 2311 and 2361 (present in SBV-UK and CSBV) but was similar to that of the Korean SBV strain that infects A. cerana (AcSBV-Kor). The differences in the AmSBV-Kor19 strain may be the result of the virus adapting to a different host.
authors: Parasitology and Insect Disease Research Laboratory, Animal, Plant and Fisheries Quarantine and Inspection Agency, 480 Anyang 6 dong, Anyang city 420-480, Republic of Korea.
authors: Se E
authors: SE
authors: Choe
authors: Lien T K
authors: LT
authors: Nguyen
authors: Jin H
authors: JH
authors: Noh
authors: Chang H
authors: CH
authors: Kweon
authors: Kondreddy E
authors: KE
authors: Reddy
authors: Hong B
authors: HB
authors: Koh
authors: Ki Y
authors: KY
authors: Chang
authors: Seung W
authors: SW
authors: Kang
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D012367:RNA, Viral; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific