title: Porphyromonas gingivalis accelerates neointimal formation after arterial injury.
abstract: BACKGROUND
  Inflammation plays a key role in neointimal hyperplasia after an arterial injury. Chronic infectious disorders, such as periodontitis, are associated with an increased risk of cardiovascular diseases. However, the effects of a periodontal infection on vascular remodeling have not been examined. We assess the hypothesis that periodontal infection could promote neointimal formation after an arterial injury.
  
  
  METHODS
  Mice were implanted with subcutaneous chambers (n = 41). Two weeks after implantation, the femoral arteries were injured, and Porphyromonas gingivalis (n = 21) or phosphate-buffered saline (n = 20) was injected into the chamber. The murine femoral arteries were obtained for the histopathological analysis. The expression level of mRNA in the femoral arteries was analyzed using quantitative reverse transcriptase polymerase chain reaction (n = 19-20).
  
  
  RESULTS
  The intima/media thickness ratio in the P. gingivalis infected group was found to be significantly increased in comparison to the non-infected group. The expression of matrix metalloproteinase-2 mRNA was significantly increased in the P. gingivalis infected group compared to the non-infected group.
  
  
  CONCLUSIONS
  These findings demonstrate that P. gingivalis injection can promote neointimal formation after an arterial injury. Periodontitis may be a critical factor in the development of restenosis after arterial intervention.
authors: Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Naho
authors: N
authors: Kobayashi
authors: Jun-ichi
authors: J
authors: Suzuki
authors: Masahito
authors: M
authors: Ogawa
authors: Norio
authors: N
authors: Aoyama
authors: Tomoya
authors: T
authors: Hanatani
authors: Yasunobu
authors: Y
authors: Hirata
authors: Ryozo
authors: R
authors: Nagai
authors: Yuichi
authors: Y
authors: Izumi
authors: Mitsuaki
authors: M
authors: Isobe
mesh_terms: D000818:Animals; D016866:Bacteroidaceae Infections / Q000378:metabolism* / Q000473:pathology; D005263:Femoral Artery / Q000293:injuries*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D051379:Mice; D058426:Neointima / Q000382:microbiology / Q000473:pathology*; D010518:Periodontitis / Q000150:complications; D016966:Porphyromonas gingivalis / Q000276:immunology; D017539:Tunica Intima / Q000473:pathology
chemical_list: D020778:Matrix Metalloproteinase 2