title: In-depth proteomic analysis of nonsmall cell lung cancer to discover molecular targets and candidate biomarkers.
abstract: Advances in proteomic analysis of human samples are driving critical aspects of biomarker discovery and the identification of molecular pathways involved in disease etiology. Toward that end, in this report we are the first to use a standardized shotgun proteomic analysis method for in-depth tissue protein profiling of the two major subtypes of nonsmall cell lung cancer and normal lung tissues. We identified 3621 proteins from the analysis of pooled human samples of squamous cell carcinoma, adenocarcinoma, and control specimens. In addition to proteins previously shown to be implicated in lung cancer, we have identified new pathways and multiple new differentially expressed proteins of potential interest as therapeutic targets or diagnostic biomarkers, including some that were not identified by transcriptome profiling. Up-regulation of these proteins was confirmed by multiple reaction monitoring mass spectrometry. A subset of these proteins was found to be detectable and differentially present in the peripheral blood of cases and matched controls. Label-free shotgun proteomic analysis allows definition of lung tumor proteomes, identification of biomarker candidates, and potential targets for therapy.
authors: Division of Hematology and Oncology, Department of Medicine, Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
authors: Takefumi
authors: T
authors: Kikuchi
authors: Mohamed
authors: M
authors: Hassanein
authors: Joseph M
authors: JM
authors: Amann
authors: Qinfeng
authors: Q
authors: Liu
authors: Robbert J C
authors: RJ
authors: Slebos
authors: S M Jamshedur
authors: SM
authors: Rahman
authors: Jacob M
authors: JM
authors: Kaufman
authors: Xueqiong
authors: X
authors: Zhang
authors: Megan D
authors: MD
authors: Hoeksema
authors: Bradford K
authors: BK
authors: Harris
authors: Ming
authors: M
authors: Li
authors: Yu
authors: Y
authors: Shyr
authors: Adriana L
authors: AL
authors: Gonzalez
authors: Lisa J
authors: LJ
authors: Zimmerman
authors: Daniel C
authors: DC
authors: Liebler
authors: Pierre P
authors: PP
authors: Massion
authors: David P
authors: DP
authors: Carbone
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009367:Neoplasm Staging; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins