title: A frameshift mutation in the canine HEXB gene in toy poodles with GM2 gangliosidosis variant 0 (Sandhoff disease).
abstract: GM2 gangliosidosis variant 0 (Sandhoff disease, SD) is a fatal, progressive neurodegenerative lysosomal storage disease caused by mutations in the HEXB gene. Toy poodles recently were reported as the second breed of dog with SD. The present paper describes the molecular defect of this canine SD as the first identification of a pathogenic mutation in the canine HEXB gene. Genomic and complementary DNA sequences covering exonic regions of the canine HEXB gene, except exon 1, were analysed using DNA and RNA in an affected dog. A homozygous single base pair deletion of guanine in exon 3 was identified at nucleotide position 283 of the putative open reading frame (c.283delG). This mutation has the potential to cause a frameshift resulting in the alteration of valine at amino acid position 59 to a stop codon (p.V59fsX). Genotyping using the mutagenically separated PCR method demonstrated a correlation between phenotype and genotype in dogs with a pedigree related to the disease and that the mutation was rare in a randomly-selected population of toy poodles. These results strongly suggest that the deletion is pathogenic.
authors: Laboratory of Clinical Pathology, Department of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima 890-0065, Japan.
authors: Mohammad M
authors: MM
authors: Rahman
authors: Hye-Sook
authors: HS
authors: Chang
authors: Keijiro
authors: K
authors: Mizukami
authors: Mohammad A
authors: MA
authors: Hossain
authors: Akira
authors: A
authors: Yabuki
authors: Shinji
authors: S
authors: Tamura
authors: Masato
authors: M
authors: Kitagawa
authors: Sawane
authors: S
authors: Mitani
authors: Takashi
authors: T
authors: Higo
authors: Mohammad M
authors: MM
authors: Uddin
authors: Kazuyuki
authors: K
authors: Uchida
authors: Osamu
authors: O
authors: Yamato
mesh_terms: D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D004283:Dog Diseases / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D005091:Exons; D016368:Frameshift Mutation; D054819:Hexosaminidase B / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D012313:RNA / Q000032:analysis; D012497:Sandhoff Disease / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*
chemical_list: D018076:DNA, Complementary; D012313:RNA; D054819:Hexosaminidase B