title: Optimization of platelet concentrate quality: application of proteomic technologies to donor management.
abstract: Quality management of blood products is essential for blood banking. It is influenced by both processing and donor characteristics and assured by monitoring routine in vitro parameters to defined product specifications. However, these measures correlate poorly with the in vivo behavior of transfused platelets and cannot be used to select optimal donors. Since radiolabeled platelet recovery and survival studies are expensive and time consuming, there is an ongoing search for simpler measures that predict platelet transfusion outcomes. We performed a pilot study using semi-qualitative proteomics to assess changes in the platelet protein profile of donors with either acceptable or unacceptable in vivo radiolabeled autologous platelet recovery and survival measurements. Proteins changing during a 9-day storage period included cytoskeletal elements talin, vinculin and moesin as well as signal transduction proteins 14-3-3, RhoGDI and Rap1. Two of nine donations exhibited a decrease in these proteins and poor in vivo platelet recovery and survival whereas the remaining donors showed acceptable platelet recovery and survival and expected protein profiles. Analyses revealed a significant correlation between protein levels of Rap1 and RhoGDI during storage and platelet recovery and survival. This study provides for the first time preliminary data showing evidence of the utility of protein profiling to predict platelet transfusion quality. This article is part of a Special Issue entitled: Integrated omics.
authors: Canadian Blood Services, Research and Development, Vancouver, BC, Canada.
authors: Peter
authors: P
authors: Schubert
authors: Brankica
authors: B
authors: Culibrk
authors: Simrath
authors: S
authors: Karwal
authors: Sherrill J
authors: SJ
authors: Slichter
authors: Dana V
authors: DV
authors: Devine
mesh_terms: D001782:Blood Donors; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001793:Blood Preservation; D001798:Blood Proteins / Q000378:metabolism*; D002470:Cell Survival; D005260:Female; D006801:Humans; D008297:Male; D017713:Platelet Transfusion; D040901:Proteomics / Q000379:methods*; D011786:Quality Control; D013997:Time Factors
chemical_list: D001798:Blood Proteins