title: [The multiplex polymerase chain reaction for detection of ventilator-associated trachea-bronchitis and pneumonia in patients with common pathogens].
abstract: OBJECTIVE
  To explore the clinical utility of multiple polymerase chain reaction (M-PCR) in the rapid detection of the common pathogens in ventilator-associated trachea - bronchitis (VAT) and ventilator-associated pneumonia (VAP).
  
  
  METHODS
  Sputum samples of 75 patients complicated VAT or VAP in surgical intensive care unit (SICU), were examined by bacterial culture, ordinary PCR, the M-PCR detection. The pathogen detection rates among three methods were compared.
  
  
  RESULTS
  The Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae of the positive detection rates were 50.7%, 45.3%, 30.7%, 41.3% and 58.7% by bacterial culture. By ordinary PCR, the positive detection rates were respectively 88.0%, 89.3%, 78.7%, 85.3% and 93.3%, and by M-PCR, the positive detection rates were respectively 92.1%, 90.7%, 82.7%, 89.3% and 96.0%. The positive rates of five common pathogens of ordinary PCR and M-PCR were higher than those of bacterial culture (all P < 0.05). The M-PCR had merit for rapid detection compared with ordinary PCR.
  
  
  CONCLUSIONS
  Compared with bacterial culture, ordinary PCR and M-PCR yield higher positive rates in identifying five common pathogens of VAT and VAP, meanwhile, it also demonstrated the tendency that M-PCR may save cost and labor power.
authors: Intensive Care Unit, Second Hospital of Lanzhou University, Lanzhou, Gansu, China.
authors: Xiao-hong
authors: XH
authors: Wang
authors: Chen-ming
authors: CM
authors: Dong
authors: Zhao-hui
authors: ZH
authors: Yang
authors: Hong-song
authors: HS
authors: Zhang
authors: Cheng-hua
authors: CH
authors: Mou
authors: Hong
authors: H
authors: Zhang
mesh_terms: D040981:Acinetobacter baumannii / Q000302:isolation & purification; D000328:Adult; D000368:Aged; D001991:Bronchitis / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism*; D004926:Escherichia coli / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D053717:Pneumonia, Ventilator-Associated / Q000175:diagnosis / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000302:isolation & purification; D055815:Young Adult