title: Possible roles of LysR-type transcriptional regulator (LTTR) homolog as a global regulator in Cronobacter sakazakii ATCC 29544.
abstract: Cronobacter sakazakii is a Gram-negative opportunistic pathogen that causes necrotizing enterocolitis, sepsis, and meningitis in premature infants. The genetic basis of C. sakazakii virulence is poorly understood. In this study, the putative LysR-type transcriptional regulator (LTTR) gene (ESA_01081 homolog) was characterized as a possible regulator for C. sakazakii pathogenesis. An in-frame deletion mutant of the ESA_01081 homolog and its cognate complementation strain were constructed and characterized for pathogenesis (adhesion/invasion potentials to human intestinal cells and in vivo rat pup challenge assay), biofilm formation, resistance to oxidative stress and induction of IL-8 secretion. LTTR-deficient C. sakazakii ATCC 29544 exhibits significantly attenuated phenotypes in all the properties tested, except adhesion. Our data strongly suggest that the putative gpESA_01081 homolog, plays an important regulatory role in diverse biological processes including the virulence of C. sakazakii. This is the first report of a functional global regulator in C. sakazakii.
authors: Department of Food and Animal Biotechnology, Center for Agricultural Biomaterials, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors: Younho
authors: Y
authors: Choi
authors: Kwang-Pyo
authors: KP
authors: Kim
authors: Kyumson
authors: K
authors: Kim
authors: Jeongjoon
authors: J
authors: Choi
authors: Hakdong
authors: H
authors: Shin
authors: Dong-Hyun
authors: DH
authors: Kang
authors: Sangryeol
authors: S
authors: Ryu
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms; D018938:Caco-2 Cells; D019295:Computational Biology / Q000379:methods; D044083:Cronobacter sakazakii / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D018384:Oxidative Stress; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C508606:CXCL8 protein, human; D016209:Interleukin-8; D014157:Transcription Factors; C073470:LysR protein, Bacteria