title: Comprehensive characterization of chondrocyte cultures in plasma and whole blood biomatrices for cartilage tissue engineering.
abstract: Many synthetic polymers and biomaterials have been used as matrices for 3D chondrocyte seeding and transplantation in the field of cartilage tissue engineering. To develop a fully autologous carrier for chondrocyte cultivation, we examined the feasibility of allogeneic plasma and whole blood-based matrices and compared them to agarose constructs. Primary articular chondrocytes isolated from 12-month-old pigs were embedded into agarose, plasma and whole blood matrices and cultivated under static-free swelling conditions for up to four weeks. To evaluate the quality of the synthesized extracellular matrix (ECM), constructs were subjected to weekly examinations using histological staining, spectrophotometry, immunohistochemistry and biochemical analysis. In addition, gene expression of cartilage-specific markers such as aggrecan, Sox9 and collagen types I, II and X was determined by RT-PCR. Chondrocyte morphology was assessed via scanning electron microscopy and viability staining, including proliferation and apoptosis assays. Finally, (13)  C NMR spectroscopy provided further evidence of synthesis of ECM components. It was shown that chondrocyte cultivation in allogeneic plasma and whole-blood matrices promoted sufficient chondrocyte viability and differentiation behaviour, resulting in neo-formation of a hyaline-like cartilage matrix.
authors: Department of Cell Techniques and Applied Stem Cell Biology, Center of Biotechnology and Biomedicine, University of Leipzig, Deutscher Platz 5, D-04103, Leipzig, Germany; Translational Centre for Regenerative Medicine, University of Leipzig, Philipp-Rosenthal-Str. 55, D-04105, Leipzig, Germany.
authors: Ronny M
authors: RM
authors: Schulz
authors: Marcus
authors: M
authors: Haberhauer
authors: Göran
authors: G
authors: Zernia
authors: Claudia
authors: C
authors: Pösel
authors: Christian
authors: C
authors: Thümmler
authors: Jeremy S
authors: JS
authors: Somerson
authors: Daniel
authors: D
authors: Huster
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002356:Cartilage / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D005109:Extracellular Matrix / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000378:metabolism; D010949:Plasma / Q000378:metabolism*; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D006025:Glycosaminoglycans