title: A monoclonal antibody SZ-117 that recognizes filamin A derived from tumor cells.
abstract: SZ117 is a monoclonal antibody against matrix metalloproteinase-2 (MMP-2) and exhibits anti-tumor angiogenic effect. In this study, we observed that SZ117 bound to a 280 kDa protein, which was detected in tumor cell-derived Matrigel and various tumor cells. Using immunoprecipitation, mass spectrometry analysis, and Western blot analysis, we identified the 280 kDa protein as filamin A and found that filamin A and its degraded products, notably a 53 kDa fragment, were released from a variety of tumor cells. This suggests that SZ117 is useful in the study of the pathogenesis of filamin A and that blockage of filamin A by SZ117 might contribute to the anti-tumor angiogenic effect of the monoclonal antibody.
authors: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Soochow University, Suzhou, Jiangsu, China.
authors: Zibin
authors: Z
authors: Bu
authors: Ruifang
authors: R
authors: Liu
authors: Bingxue
authors: B
authors: Shang
authors: Zhifei
authors: Z
authors: Cao
authors: Yanyan
authors: Y
authors: Pan
authors: Quansheng
authors: Q
authors: Zhou
authors: Changgeng
authors: C
authors: Ruan
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003285:Contractile Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D064448:Filamins; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D003285:Contractile Proteins; D064448:Filamins; D008840:Microfilament Proteins; D010446:Peptide Fragments; C575758:SZ-117 monoclonal antibody