title: A high-resolution 15,000(Rad) radiation hybrid panel for the domestic cat.
abstract: The current genetic and recombination maps of the cat have fewer than 3,000 markers and a resolution limit greater than 1 Mb. To complement the first-generation domestic cat maps, support higher resolution mapping studies, and aid genome assembly in specific areas as well as in the whole genome, a 15,000(Rad) radiation hybrid (RH) panel for the domestic cat was generated. Fibroblasts from the female Abyssinian cat that was used to generate the cat genomic sequence were fused to a Chinese hamster cell line (A23), producing 150 hybrid lines. The clones were initially characterized using 39 short tandem repeats (STRs) and 1,536 SNP markers. The utility of whole-genome amplification in preserving and extending RH panel DNA was also tested using 10 STR markers; no significant difference in retention was observed. The resolution of the 15,000(Rad) RH panel was established by constructing framework maps across 10 different 1-Mb regions on different feline chromosomes. In these regions, 2-point analysis was used to estimate RH distances, which compared favorably with the estimation of physical distances. The study demonstrates that the 15,000(Rad) RH panel constitutes a powerful tool for constructing high-resolution maps, having an average resolution of 40.1 kb per marker across the ten 1-Mb regions. In addition, the RH panel will complement existing genomic resources for the domestic cat, aid in the accurate re-assemblies of the forthcoming cat genomic sequence, and support cross-species genomic comparisons.
authors: Population Health and Reproduction,, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, USA.
authors: L H
authors: LH
authors: Bach
authors: B
authors: B
authors: Gandolfi
authors: J C
authors: JC
authors: Grahn
authors: L V
authors: LV
authors: Millon
authors: M S
authors: MS
authors: Kent
authors: K
authors: K
authors: Narfstrom
authors: S A
authors: SA
authors: Cole
authors: J C
authors: JC
authors: Mullikin
authors: R A
authors: RA
authors: Grahn
authors: L A
authors: LA
authors: Lyons
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000235:genetics*; D002415:Cats / Q000235:genetics*; D002459:Cell Fusion; D002460:Cell Line; D006822:Hybrid Cells; D020641:Polymorphism, Single Nucleotide