title: Hydroxyl scavenging activity accounts for differential antioxidant protection of Plantago major against oxidative toxicity in isolated rat liver mitochondria.
abstract: OBJECTIVE
  The aim of this work was to study the effects of P. major against the oxidative damage of isolated rat liver mitochondria.
  
  
  METHODS
  The extracts were obtained using methanol (MeOH), ethyl acetate (EAc), dichloromethane (DCM), and hexane (Hex) as solvents.
  
  
  RESULTS
  Hex, DCM, and EAc totally, and MeOH partially, inhibited ROS generation and lipid peroxidation of membranes induced by Fe(2+) or t-BOOH. However, only MeOH was able to prevent the t-BOOH-induced glutathione and NAD(P)H oxidation. All extracts chelated Fe(2+) and reduced DPP Hradicals. EPR analysis revealed that P. major exhibited potent scavenger activity for hydroxyl radicals.
  
  
  CONCLUSIONS
  The potent antioxidant activity exhibited by P. major was able to prevent oxidative mitochondrial damage, contributing to the understanding of its hepatoprotective action against ROS-mediated toxicity.
authors: Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, Mogi das Cruzes, SP, Brazil.
authors: Joyce C
authors: JC
authors: Mello
authors: Natalia S S
authors: NS
authors: Guimarães
authors: Mariano V D
authors: MV
authors: Gonzalez
authors: Juliana S
authors: JS
authors: Paiva
authors: Tatiana
authors: T
authors: Prieto
authors: Otaciro R
authors: OR
authors: Nascimento
authors: Tiago
authors: T
authors: Rodrigues
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D010943:Plantago; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002614:Chelating Agents; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; D009249:NADP; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron; D005978:Glutathione