title: Reversal of the extreme coenzyme selectivity of Clostridium symbiosum glutamate dehydrogenase.
abstract: Active-site mutants of glutamate dehydrogenase from Clostridium symbiosum have been designed and constructed and the effects on coenzyme preference evaluated by detailed kinetic measurements. The triple mutant F238S/P262S/D263K shows complete reversal in coenzyme selectivity from NAD(H) to NADP(H) with retention of high levels of catalytic activity for the new coenzyme. For oxidized coenzymes, k(cat) /K(m) ratios of the wild-type and triple mutant enzyme indicate a shift in preference of approximately 1.6 × 10(7) -fold, from ∼ 80,000-fold in favour of NAD(+) to ∼ 200-fold in favour of NADP(+). For reduced coenzymes the corresponding figure is 1.7 × 10(4) -fold, from ∼ 1000-fold in favour of NADH to ∼ 17-fold in favour of NADPH. A fourth mutation (N290G), previously identified as having a potential bearing on coenzyme specificity, did not engender any further shift in preference when incorporated into the triple mutant, despite having a significant effect when expressed as a single mutant.
authors: School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Ireland.
authors: Michael A
authors: MA
authors: Sharkey
authors: Alessandro
authors: A
authors: Gori
authors: Marina
authors: M
authors: Capone
authors: Paul C
authors: PC
authors: Engel
mesh_terms: D001483:Base Sequence; D048009:Clostridium symbiosum / Q000201:enzymology*; D017931:DNA Primers; D005969:Glutamate Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D016296:Mutagenesis; D013379:Substrate Specificity
chemical_list: D017931:DNA Primers; D005969:Glutamate Dehydrogenase