title: Rapid and sensitive detection of Banana bunchy top virus by loop-mediated isothermal amplification.
abstract: A sensitive loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of Banana bunchy top virus (BBTV) infection. The reaction was performed in a single tube at 63°C for 90 min, with an improved closed-tube detection system by adding the SYBR Green I dye to the inside of the tube lid prior to amplification. The detection limit of the LAMP assay was approximately 1 pg/μl plasmid DNA when mixed with extracted DNA from healthy banana plant, and no cross-reaction with other banana-infected pathogens was observed. Real-time turbidimetry was used to monitor the amplification result in the tubes, and it was shown that this LAMP assay was about 100-fold more sensitive than PCR. The results demonstrated that this LAMP method should be useful for both banana disease monitoring and mass propagation of virus-free banana plantlets.
authors: State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China.
authors: Jun
authors: J
authors: Peng
authors: Junfang
authors: J
authors: Zhang
authors: Zihao
authors: Z
authors: Xia
authors: Yongqiang
authors: Y
authors: Li
authors: Junsheng
authors: J
authors: Huang
authors: Zaifeng
authors: Z
authors: Fan
mesh_terms: D052658:Babuvirus / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D052160:Benzothiazoles; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D003959:Diamines; D016679:Genome, Viral; D008969:Molecular Sequence Data; D028521:Musa / Q000235:genetics / Q000821:virology; D009391:Nephelometry and Turbidimetry / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009930:Organic Chemicals; D010935:Plant Diseases / Q000821:virology*; D010957:Plasmids / Q000235:genetics; D011804:Quinolines; D012680:Sensitivity and Specificity; D012692:Serial Passage; D013997:Time Factors
chemical_list: D052160:Benzothiazoles; D018744:DNA, Plant; D004279:DNA, Viral; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I