title: S-nitrosothiols and the S-nitrosoproteome of the cardiovascular system.
abstract: CONCLUSIONS
  Since their discovery in the early 1990's, S-nitrosylated proteins have been increasingly recognized as important determinants of many biochemical processes. Specifically, S-nitrosothiols in the cardiovascular system exert many actions, including promoting vasodilation, inhibiting platelet aggregation, and regulating Ca(2+) channel function that influences myocyte contractility and electrophysiologic stability.
  
  
  BACKGROUND
  Contemporary developments in liquid chromatography-mass spectrometry methods, the development of biotin- and His-tag switch assays, and the availability of cyanide dye-labeling for S-nitrosothiol detection in vitro have increased significantly the identification of a number of cardiovascular protein targets of S-nitrosylation in vivo.
  
  
  RESULTS
  Recent analyses using modern S-nitrosothiol detection techniques have revealed the mechanistic significance of S-nitrosylation to the pathophysiology of numerous cardiovascular diseases, including essential hypertension, pulmonary hypertension, ischemic heart disease, stroke, and congestive heart failure, among others.
  
  
  CONCLUSIONS
  Despite enhanced insight into S-nitrosothiol biochemistry, translating these advances into beneficial pharmacotherapies for patients with cardiovascular diseases remains a primary as-yet unmet goal for investigators within the field.
authors: Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors: Bradley A
authors: BA
authors: Maron
authors: Shiow-Shih
authors: SS
authors: Tang
authors: Joseph
authors: J
authors: Loscalzo
mesh_terms: D000818:Animals; D002318:Cardiovascular Diseases / Q000378:metabolism / Q000503:physiopathology; D002319:Cardiovascular System / Q000378:metabolism* / Q000503:physiopathology; D003545:Cysteine / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism / Q000502:physiology; D015538:Nitrosation; D010084:Oxidation-Reduction; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000378:metabolism*; D026403:S-Nitrosothiols / Q000378:metabolism*
chemical_list: D020543:Proteome; D026403:S-Nitrosothiols; D009569:Nitric Oxide; D005978:Glutathione; D003545:Cysteine