title: SHG as a new modality for large field of view imaging to monitor tissue collagen network.
abstract: For this study, we have considered a new large field of view imaging dedicated to matrix collagen (no stained samples). To integrate a multidimensional scale (non-sliced samples), a femtosecond oscillator (two photon excitation laser) has been coupled with a large field optical setup to collect SHG signal. We introduced an index (F-SHG) based on decay time response measured by TCSPC for, respectively, Fluorescence (F) and Second Harmonic Generation (SHG) values. For samples where protein collagen is the major component of extracellular matrix (skin) or not (nacre), we compared the index distribution (from 2 to 12) obtained with large field optical setup. In this work, we showed for the first time that multiscale large field imaging combined to multimodality approaches (SHG-TCSPC) could be an innovative and non invasive technique to detect and identify some biological interest molecules (collagen) in biomedical topics.
authors: CNRS, UMR 7561 et FR3209, Plate Forme d'Imagerie Cellulaire et Tissulaire PTIBC-IBISA, Nancy-Université, Faculté de Médecine, Vandoeuvre-lès-Nancy, France Nancy Universités, Nancy, France. dominique.dumas@medecine.uhp-nancy.fr
authors: D
authors: D
authors: Dumas
authors: S
authors: S
authors: Hupont
authors: C
authors: C
authors: Huselstein
authors: N
authors: N
authors: de Isla
authors: M
authors: M
authors: Rousseau
authors: E
authors: E
authors: Werkmeister
authors: J
authors: J
authors: Magdalou
authors: P
authors: P
authors: Menu
authors: J-F
authors: JF
authors: Stoltz
mesh_terms: D000818:Animals; D003094:Collagen / Q000032:analysis / Q000648:ultrastructure*; D005109:Extracellular Matrix / Q000737:chemistry / Q000648:ultrastructure*; D008297:Male; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D060734:Nacre / Q000032:analysis*; D049911:Pinctada / Q000737:chemistry / Q000648:ultrastructure*; D051381:Rats; D012867:Skin / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D060734:Nacre; D003094:Collagen