title: Interaction of nucleic acids with Coomassie Blue G-250 in the Bradford assay.
abstract: The Bradford assay has been used reliably for decades to quantify protein in solution. The analyte is incubated in acidic solution of Coomassie Blue G-250 dye, during which reversible ionic and nonionic binding interactions form. Bradford assay color yields were determined for salmon, bovine, shrimp, and kiwi fruit genomic DNA; baker's yeast RNA; bovine serum albumin (BSA); and hen egg lysozyme. Pure DNA and RNA bound the dye, with color yields of 0.0017 mg⁻¹ cm⁻¹ and 0.0018 mg⁻¹ cm⁻¹, respectively. The nucleic acid-Coomassie Blue response was significant, at roughly 9% of that for BSA and 18% of that for lysozyme.
authors: Department of Chemistry and Biochemistry, Bloomsburg University of Pennsylvania, Bloomsburg, PA 17815, USA.
authors: Broc R
authors: BR
authors: Wenrich
authors: Toni A
authors: TA
authors: Trumbo
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002417:Cattle; D002645:Chickens; D004247:DNA / Q000032:analysis; D009696:Nucleic Acids / Q000378:metabolism*; D012331:RNA, Fungal / Q000032:analysis; D012015:Reference Standards; D012394:Rosaniline Dyes / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000032:analysis
chemical_list: D009696:Nucleic Acids; D012331:RNA, Fungal; D012394:Rosaniline Dyes; D012710:Serum Albumin, Bovine; D004247:DNA; C004692:coomassie Brilliant Blue