title: Molecular analysis of the mutational effects of Thai street rabies virus with increased virulence in mice after passages in the BHK cell line.
abstract: QS-BHK-P7, street rabies virus, after passages in the BHK cell line, had an in vitro phenotype that distinguished it from its parental virus. Both viruses caused lethal infection in mice by central nervous system inoculation; however, only QS-BHK-P7 killed mice by the intramuscular route. We found four mutations, S23R and H424P in ectodomain of the glycoprotein (G), I1711 V in the polymerase genes, and another at the non-coding region between the phosphoprotein and matrix protein genes of QS-BHK-P7. None of the mutations in the G gene occurred in previously reported pathogenic determinants. The roles of mutations in particular non-coding regions remain to be elucidated.
authors: WHO Collaborating Centre for Research and Training on Viral Zoonoses, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand. phatthamon@gmail.com
authors: Phatthamon
authors: P
authors: Virojanapirom
authors: Pakamatz
authors: P
authors: Khawplod
authors: Artikaya
authors: A
authors: Sawangvaree
authors: Supaporn
authors: S
authors: Wacharapluesadee
authors: Thiravat
authors: T
authors: Hemachudha
authors: Kentaro
authors: K
authors: Yamada
authors: Kinjiro
authors: K
authors: Morimoto
authors: Akira
authors: A
authors: Nishizono
mesh_terms: D000818:Animals; D002460:Cell Line; D004252:DNA Mutational Analysis; D004195:Disease Models, Animal; D004285:Dogs; D051379:Mice; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D022661:RNA, Untranslated / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D011818:Rabies / Q000401:mortality / Q000821:virology*; D011820:Rabies virus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D017422:Sequence Analysis, DNA; D012692:Serial Passage; D016019:Survival Analysis; D013785:Thailand; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence
chemical_list: D022661:RNA, Untranslated; D012367:RNA, Viral; D014764:Viral Proteins