Chromatographic determination of methyl glyoxal in blood plasma as the test for glycotoxicity and accumulation of glycation end-products.
We developed a method of measuring methyl glyoxal concentration in blood serum using HPLC with UV detection. Methyl glyoxal concentration was determined in healthy subjects. The method was developed for indirect but reliable measurement of the levels of glycation end-products in patients with diabetes, hyperlipidemia, and cardiovascular pathologies.
Laboratory of Clinical Biochemistry of Lipid Metabolism, Russian Cardiology Research-and-Production Complex, Ministry of Health Care and Social Development of Russian Federation, Moscow, Russia. shiju@mail.ru
Yu K
YK
Shiryaeva
V V
VV
Krylin
V N
VN
Titov
D000293:Adolescent; D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood*; D006031:Glycosylation; D006037:Glyoxal / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry*; D055815:Young Adult
D017127:Glycation End Products, Advanced; D006037:Glyoxal