[The effects of activin A and inhibin A on the IVM of mice immature oocytes].
OBJECTIVE
To study the effects of activin A (ACTA) and inhibin A (INHA) on the in vitro maturation (IVM) of mice immature eggs and early embryonic development.


METHODS
1. The mice oocytes were cultured in the culture medium contained different concentration of ACTA and INHA (50 ng/mL, 100 ng/mL, 200 ng/mL) to find the optimum concentration; 2. The effects of different concentration of ACTA, INHA, ACTA+ INHA on the mature rates of mice oocytes were compared; 3. The effects of different concentration of ACTA, INHA, ACTA+INHA on the fertility rates and blastocysts formation rates of IVM mice eggs were also compared.


RESULTS
The mature rates of mice oocytes in culture medium contained (100 ng/mL and 200 ng/mL) ACTA and INHA were significantly higher than that contained 50 ng/mL ACTA and INHA (P < 0.05); There was no difference between 100 ng/mL and 200 ng/mL groups. The mature rates, fertility rates and blastocyst rates of mice oocytes were significantly higher than those of control (P < 0.05).


CONCLUSIONS
The mature rates, fertility rates and embryonic development potential after fertilization of mice oocytes were significantly promoted by ACTA, INHA. The optimum concentration of ACTA and INHA in culture medium was 100 ng/mL.
Reproductive Medicine Laboratory of the Second Clinical Medical College, Chengdu University of Traditional Chinese Medicine, Chengdu 610041, China.
Lun
L
Zhang
Xin
X
Liang
Xiao-hui
XH
Jiang
D028341:Activins / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005307:Fertilization in Vitro; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods*; D007265:Inhibins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*
C422405:activin A; C422362:inhibin A; D028341:Activins; D007265:Inhibins