Sinomenine can prolong high-risk corneal graft survival in a rat model.
OBJECTIVE
To study the role of sinomenine (SIN) in prolonging high-risk corneal graft survival in rats.


METHODS
All recipients were randomly assigned to SIN, cyclosporine A (CsA), SIN plus CsA and control groups. IL-2, IL-10, Fas-Fas ligand and CD4(+)CD25(+)FoxP3(+) T cells in peripheral blood were detected. In addition, rat corneal grafts' survival times were recorded.


RESULTS
Survival time was 15.88 ± 5.87 days in the SIN group, 17.67 ± 5.43 days in the CsA group and 20.75 ± 4.77 days in the drug combination group, which were longer survival times than those in the control group (p < 0.05). Compared with the SIN and CsA groups, levels of CD4(+)CD25(+)FoxP3(+) lymphocytes in the control group were decreased (p < 0.05) and were increased in the cotreated group (p < 0.05). IL-2 levels in the SIN-only and CsA-only groups were lower than those in the control group (p < 0.05) and higher than those in the cotreated group (p < 0.05); however, the results for IL-10 were different. The expressions of Fas and Fas ligand were least in the control group.


CONCLUSIONS
SIN could prolong allograft survival and might have potential clinical usage.
Beijing Chaoyang Hospital, Capital Medical University, Beijing, People's Republic of China.
Ying
Y
Wang
Dongli
D
Ma
Ying
Y
Jie
Yuying
Y
Wu
Zhiqiang
Z
Pan
D000818:Animals; D015704:CD4 Antigens / Q000096:biosynthesis; D016039:Corneal Transplantation; D016572:Cyclosporine / Q000008:administration & dosage / Q000009:adverse effects; D004359:Drug Therapy, Combination; D005260:Female; D051858:Forkhead Transcription Factors / Q000096:biosynthesis; D006084:Graft Rejection / Q000209:etiology / Q000517:prevention & control*; D006085:Graft Survival / Q000187:drug effects*; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis; D023421:Models, Animal; D009019:Morphinans / Q000008:administration & dosage* / Q000009:adverse effects; D051381:Rats; D011917:Rats, Inbred Lew; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology
D015704:CD4 Antigens; D051858:Forkhead Transcription Factors; C500522:Foxp3 protein, rat; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D009019:Morphinans; D016753:Interleukin-10; C009271:sinomenine; D016572:Cyclosporine