Extraction of high-quality RNA from rubber tree leaves.
A specific technique capable of producing high-quality RNA for rapid amplification of cDNA ends (RACE) was established for challenging tissues: leaves of the rubber tree. Total RNA was extracted by cetyltrimethylammonium bromide (CTAB)-LiCl combined with TRIzol reagent. The isolated RNA was highly intact. With RNA as template, full-length cDNA was obtained (NCBI, AY461413) by RACE.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Liu-Hong
LH
Deng
Ming-Wu
MW
Luo
Chun-Fa
CF
Zhang
Hui-Cai
HC
Zeng
D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D030541:Databases, Genetic; D004587:Electrophoresis, Agar Gel; D006146:Guanidines / Q000737:chemistry; D028482:Hevea / Q000737:chemistry* / Q000235:genetics; D018021:Lithium Chloride / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques; D010636:Phenols / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry* / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification*
D002593:Cetrimonium Compounds; D018076:DNA, Complementary; D006146:Guanidines; D010636:Phenols; D018749:RNA, Plant; C411644:trizol; D018021:Lithium Chloride; D000077286:Cetrimonium