Phytoceramides and acylated phytosterol glucosides from Pterospermum acerifolium Willd. seed coat and their osteogenic activity.
Phytochemical investigation of seed coats of Pterospermum acerifolium afforded two phytoceramides (1, 2) and two acylated phytosterol glucosides (3, 4) together with five known compounds (5-9). Their structures were elucidated on the basis of extensive spectroscopic analysis using 1D, 2D NMR and Mass spectrometry. Compounds 1, 2, 3, and 4 were assessed for their osteogenic activity using primary cultures of osteoblasts harvested from neonatal rat calvaria. Among these compounds, 1 and 2 markedly stimulated osteoblast differentiation assessed by alkaline phosphatase production and osteoblast mineralization by alizarin red-S staining.
Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow 226001, India.
Preety
P
Dixit
Kailash
K
Chand
Mohd Parvez
MP
Khan
Jawed Akhtar
JA
Siddiqui
Deepshikha
D
Tewari
Florence Tsofack
FT
Ngueguim
Naibedya
N
Chattopadhyay
Rakesh
R
Maurya
D000215:Acylation; D000469:Alkaline Phosphatase / Q000032:analysis / Q000737:chemistry; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry; D001854:Bone Marrow Cells / Q000187:drug effects; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D002518:Ceramides / Q000737:chemistry* / Q000494:pharmacology; D050659:Core Binding Factor Alpha 1 Subunit / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D019660:Malvaceae / Q000737:chemistry*; D015394:Molecular Structure; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis; D010840:Phytosterols / Q000737:chemistry* / Q000494:pharmacology; D061251:Primary Cell Culture; D051381:Rats; D012639:Seeds / Q000737:chemistry*; D012886:Skull / Q000737:chemistry / Q000187:drug effects
D000880:Anthraquinones; D055396:Bone Morphogenetic Protein 2; D002518:Ceramides; D050659:Core Binding Factor Alpha 1 Subunit; D005960:Glucosides; D010840:Phytosterols; C493717:Runx2 protein, rat; C004468:Alizarin Red S; D000469:Alkaline Phosphatase