Construction of swine-specific CpG motif enriched plasmid and the study of its immunostimulatory effects both in vitro and in vivo.
A swine-specific CpG motif enriched plasmid (pUC18-CpG) was constructed in this study. Its immunostimulant property was tested in vitro via lymphocyte transformation assay using swine peripheral blood mononuclear cells (PBMCs). The recombinant plasmid showed higher Stimulation Index (SI) compared to the positive control (LPS). In a following animal experiment, pUC18-CpG was co-administered with a commercial swine FMD killed vaccine. Animals in the pUC18-CpG adjuvanted groups showed much higher antibody titers during the vaccination period.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Xiaoyu
X
Guo
Hong
H
Jia
Weifeng
W
Yuan
Quan
Q
Zhang
Shaohua
S
Hou
Yao
Y
Sun
Gang
G
Zhu
Hongfei
H
Zhu
José Manuel
JM
Sánchez-Vizcaíno
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018899:CpG Islands / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control*; D007114:Immunization / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D010957:Plasmids / Q000235:genetics / Q000494:pharmacology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology*
D000914:Antibodies, Viral; D009838:Oligodeoxyribonucleotides