Single step isolation and activation of primary CD3+ T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers.
Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP-NF) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP-NF to specifically isolate, enrich, and activate CD3(+) T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8(+)CD3(+) T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP-NF permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications.
Global Research Lab, Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul 136-713, Korea.
Kwanghee
K
Kim
Hyo Jin
HJ
An
Seung-Hyun
SH
Jun
Tae-Jin
TJ
Kim
Seon Ah
SA
Lim
Gayoung
G
Park
Hyon Bin
HB
Na
Yong Il
YI
Park
Taeghwan
T
Hyeon
Cassian
C
Yee
Jeffrey A
JA
Bluestone
Jungbae
J
Kim
Kyung-Mi
KM
Lee
D000438:Alcohols; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods*; D008213:Lymphocyte Activation; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D057139:Nanofibers / Q000737:chemistry*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*
D000438:Alcohols; D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D058185:Magnetite Nanoparticles