Congratulations, you have been carefully chosen to represent an important developmental regulator!
BACKGROUND
Studying a process in a new species often relies on focusing our attention to a candidate gene, encoding a protein similar to one with a known function. Not all the choices seem to be prudent.


METHODS
This Viewpoint includes an overview of issues that are encountered during research of candidate genes. Defining a match for a gene of interest, deciding whether variation in ESTs or RNAseq data for a certain transcript, represent more than one gene. The problem of incorrect annotation of genes due to incorrect in-silico splicing, is also mentioned. The author's humble opinion on how to deal with these issues is provided.


CONCLUSIONS
The vast amount of new sequence data provides us with great possibilities for giant leaps in our understanding. Still, we cannot afford to skip over the tedious steps required to confirm that we are indeed studying the correct gene, and try to be sure that the complex expression pattern we observe is not a composite of several genes.
The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, 76100, Israel. samach@agri.huji.ac.il
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Samach
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics; D020869:Gene Expression Profiling; D017343:Genes, Plant; D027845:Malus / Q000235:genetics; D058977:Molecular Sequence Annotation; D010944:Plants / Q000235:genetics*
D017931:DNA Primers