Analysis and comparison of the microflora isolated from fresco surface and from surrounding air environment through molecular and biodegradative assays.
The aim of this study was to find a correlation among the environmental isolated microflora and the fresco colonizators through the investigation of their biodegradative abilities and DNA characteristics. A molecular technique named RAMP (Random Amplified Microsatellite Polymorphisms) was utilized in order to analyze the DNA diversity of bacterial and fungal species isolated from fresco as well as from air samples. The RAMP-PCR results were combined with the screening of some biodegradative properties obtained through the use of specific agar plate assays detecting the proteolytic, solubilization and biomineralization abilities of the isolated microflora. This comparative analysis showed that only in few cases a direct link among the fresco and airborne isolates of specific microbial group existed. The investigation clearly evidenced that colonization of surface of Ladislav's fresco occurred in different time and by different strains than those observed at the moment of sampling campaign. Furthermore, the microflora investigation permitted the identification of taxonomically interesting bacteria with particular biodegradative properties, which had been less studied until now.
Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 84551 Bratislava, Slovak Republic. domenico.pangallo@savba.sk
Domenico
D
Pangallo
Lucia
L
Kraková
Katarína
K
Chovanová
Alexandra
A
Simonovičová
Filomena
F
De Leo
Clara
C
Urzì
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D058448:Biota; D001711:Biotransformation; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004783:Environmental Microbiology; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014644:Genetic Variation; D053858:Metabolic Networks and Pathways; D054892:Metagenome; D010151:Paintings; D016133:Polymerase Chain Reaction; D059748:Proteolysis
D004269:DNA, Bacterial; D004271:DNA, Fungal