Acyl-CoA binding domain containing 3 (ACBD3) recruits the protein phosphatase PPM1L to ER-Golgi membrane contact sites.
The metal-dependent protein phosphatase family (PPM) governs a number of signaling pathways. PPM1L, originally identified as a negative regulator of stress-activated protein kinase signaling, was recently shown to be involved in the regulation of ceramide trafficking at ER-Golgi membrane contact sites. Here, we identified acyl-CoA binding domain containing 3 (ACBD3) as an interacting partner of PPM1L. We showed that this association, which recruits PPM1L to ER-Golgi membrane contact sites, is mediated by a GOLD (Golgi dynamics) domain in ACBD3. These results suggested that ACBD3 plays a pivotal role in ceramide transport regulation at the ER-Golgi interface.
Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, Japan.
Yasuharu
Y
Shinoda
Kohsuke
K
Fujita
Satoko
S
Saito
Hiroyuki
H
Matsui
Yusuke
Y
Kanto
Yuko
Y
Nagaura
Kohji
K
Fukunaga
Shinri
S
Tamura
Takayasu
T
Kobayashi
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D008958:Models, Molecular; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D033921:Vesicular Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
C449459:ACBD3 protein, human; D048868:Adaptor Proteins, Signal Transducing; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C114051:VAPA protein, human; D033921:Vesicular Transport Proteins; C119090:CERT1 protein, human; D017346:Protein Serine-Threonine Kinases; C521060:PPM1L protein, human; D010749:Phosphoprotein Phosphatases