Comparative proteomics analysis of colorectal cancer.
OBJECTIVE
Protein expression in colon and rectal cancer (CRC) and paired normal tissues was examined by two-dimensional gel electrophoresis (2-DE) to identify differentially expressed proteins.


METHODS
Five fresh colorectal cancer and paired adjacent normal tissues were obtained and differentially expressed protein spots were determined using PDQuest software, with identification on the basis of MALDI- TOF mass spectra.


RESULTS
Compared with normal colorectal mucosa, protein abnormal expression of 65 spots varying more than 1.5 times were found in 2-DE gels from colorectal cancer samples (P<0.05); forty-two proteins were up-regulated and 23 were down-regulated; twelve protein spots were identified using mass spectrometry, of which 8 were up-regulated, including HSPB1 and Annexin A4, while 4 were down-regulated, the results being consistent with Western blot findings.


CONCLUSIONS
Two-dimensional electrophoresis reference maps for CRC tissues and adjacent normal mucosa (NMC) were established and 12 differentially expressed proteins were identified. Up-regulated HSPB1 and Annexin A4 may play many important roles in the pathogenesis of colorectal cancer.
Department of Gastrointestinal Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou, China.
Jun-Jiang
JJ
Wang
Ying
Y
Liu
Yang
Y
Zheng
Feng
F
Lin
Guan-Fu
GF
Cai
Xue-Qing
XQ
Yao
D017314:Annexin A4 / Q000378:metabolism; D003106:Colon / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D018832:Molecular Chaperones; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D012007:Rectum / Q000378:metabolism*; D015854:Up-Regulation
D017314:Annexin A4; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D011506:Proteins