Immobilization of engineered arginase on gold-carbon nanotubes.
It is demonstrated that engineered arginase with a single protruded site of cysteine deliberately placed away from its active centre by site-directed mutagenesis can facilitate its attachment on a gold-nanoparticle surface with atomic precision, resulting in no apparent loss in enzymatic activity.
Department of Chemistry, University of Oxford, Oxford, OX1 3QR, UK.
Gordon Kuen-Chan
GK
Lee
Sui-Yi
SY
Kwok
Chih Hao
CH
Yu
Kin
K
Tam
Hiu-Chi
HC
Chong
Yun-Chung
YC
Leung
Shik Chi Edman
SC
Tsang
D000327:Adsorption; D000970:Antineoplastic Agents / Q000138:chemical synthesis*; D001119:Arginase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001120:Arginine / Q000378:metabolism*; D001407:Bacillus / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D007700:Kinetics; D018625:Microscopy, Atomic Force; D016297:Mutagenesis, Site-Directed; D037742:Nanotubes, Carbon / Q000737:chemistry; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013499:Surface Properties
D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D011994:Recombinant Proteins; D006046:Gold; D001120:Arginine; D001119:Arginase; D003545:Cysteine