Detection and prognostic analysis of serum protein expression in esophageal squamous cell cancer.
OBJECTIVE
To assess differences in serum proteins in esophageal squamous cell carcinoma patients.


METHODS
144 esophageal squamous cell carcinoma patients and 50 healthy volunteers were included in this study, with surface-enhanced laser desorption-ionization time-of-flight mass spectrometry and weak cation exchange magnetic beads. Follow-up allowed the relations between serum proteins and prognosis to be analyzed.


RESULTS
A total of 93 protein peaks were detected (molecular weight range: 1500-3000), 10 demonstrating statistically significant differences. There were no differences in protein peaks between 92 patients with a survival more than 2 years and 52 patients with survival less than 2 years. There were two significantly different protein peaks between 45 stage II patients with a survival more than 2 years and 14 stage II patients with survival less than 2 years. There was one significantly different protein peak between 22 stage III patients with a survival more than 2 years and 29 stage III patients with survival less than 2 years.


CONCLUSIONS
Differences of serum proteins in esophageal squamous cell carcinoma are related to prognosis of patients. The protein fingerprint can be helpful for clinical diagnosis and treatment.
Key Laboratory of Thoracic Tumor Diagnosis(Esophagus, Lung) and Treatment Technology Research, Department of Thoracic Surgery, Zhejiang Cancer Institue, Hangzhou, China.
Hong
H
Jiang
Xiao-Hong
XH
Wang
Xin-Min
XM
Yu
Zhi-Guo
ZG
Zheng
D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000097:blood*; D004938:Esophageal Neoplasms / Q000097:blood*; D006801:Humans; D008875:Middle Aged; D011379:Prognosis; D012372:ROC Curve; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016019:Survival Analysis
D014408:Biomarkers, Tumor; D001798:Blood Proteins