A rapid and cost effective method in purifying small RNA.
Purification of RNA fragments from a complex mixture is a very common technique, and requires consideration of the time, cost, purity and yield of the purified RNA fragments. This study describes the fastest method of purifying small RNA with the lowest cost possible, without compromizing the yield and purity. The technique describes the purification of small RNA from polyacrylamide gel, resulting in a good yield of small RNA with minimum experimental steps in avoiding degradation of the RNA, obviating the use of ethidium bromide and phenol-chloroform extraction, as well as siliconized glass wools to remove the polyacrylamide gel particles. The purified small RNA is suitable for a wide variety of applications such as ligation, end labelling with radio isotope, RT-PCR (Reverse Transcriptase-PCR), Northern blotting, experimental RNomics study and also Systematic Evolution of Ligands by Exponential Enrichment (SELEX).
Infectious Disease Cluster, Advanced Medical & Dental Institute, Universiti Sains Malaysia, 13200, Kepala Batas, Penang, Malaysia.
Marimuthu
M
Citartan
Soo-Choon
SC
Tan
Thean-Hock
TH
Tang
D000180:Acrylic Resins; D001483:Base Sequence; D001709:Biotechnology; D003362:Cost-Benefit Analysis; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D052156:SELEX Aptamer Technique
D000180:Acrylic Resins; C016680:polyacrylamide gels; D012313:RNA