[Determination of twelve ginsenosides in Panax ginseng by HPLC].
OBJECTIVE
To determine the contents of twelve ginsenosides in the root of Panax ginseng by HPLC.


METHODS
The analysis is carried out at room temperature on a Luna NH2 column (4.6 mm x 150 mm, 5 microm) eluted with acetonitrile and water as the mobile phases in a gradient elution. The flow-rate was 1.0 mL x min(-1), the detection wavelength was 203 nm.


RESULTS
Twelve ginsenosides (Rh2, Rh1, Rg2, Rg3, Rg1, Rf, Re, Rd, Re, Rb2, Rb3, Rb1) were separated at baseline within 60 min with good linearity (r > or = 0.999 5). The recovery rates were 98.1%, 95.3%, 96.1%, 95.6%, 97.3%, 98.6%, 98.0%, 96.4%, 96.1%, 97.6%, 96.8%, 96.9% (RSD < or = 3.0%).


CONCLUSIONS
The method was simple,fast and could control the quality of P. ginseng effectively.
College of Pharmaceutical and Biological Engineering, Chongqing University of Technology, Chongqing 400054, China. yxx651116@cqut.edu.cn
Xiuying
X
Xu
Yimin
Y
Zheng
Shanquan
S
Fu
Ying
Y
Zhao
Jie
J
Li
Linlin
L
Wang
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000032:analysis*; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry*; D011786:Quality Control
D036145:Ginsenosides; D010936:Plant Extracts