Assessment of serological tests for the diagnosis of canine visceral leishmaniasis.
An immunoenzymatic assay (ELISA), an indirect immunofluorescence antibody test (IFAT) with different antigens (ELISA-Leishmania chagasi, ELISA-L. major-like, IFAT-L. chagasi and IFAT-L. major-like), and an immunochromatographic test were assessed for the diagnosis of canine visceral leishmaniasis (CVL). Serum samples from 144 dogs from an endemic area for visceral leishmaniasis in the municipality of Rio de Janeiro were tested. The sensitivities of the serological tests were 93%, 100%, 73%, 60% and 93%, with specificities of 87%, 92%, 77%, 96% and 92% for the ELISA-L. major-like, ELISA-L. chagasi, IFAT-L. major-like, IFAT-L. chagasi and the immuno chromatographic test, respectively. ELISA-L. chagasi was the best test for the diagnosis of CVL, but the immunochromatographic test could be a useful alternative as it offers simple and rapid diagnosis without the need for a specialized laboratory.
Pós-graduação Stricto-sensu, Instituto de Pesquisa Clinica Evandro Chagas IPEC, Fundação Oswaldo Cruz FIOCRUZ, Rio de Janeiro, Brazil. denise.silva@ipec.fiocruz.br
Denise Amaro
DA
da Silva
Maria de Fátima
Mde F
Madeira
Tuanne Rotti
TR
Abrantes
Carlos José de Lima Barbosa
CJ
Filho
Fabiano Borges
FB
Figueiredo
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000662:veterinary; D007891:Leishmania / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary*