Effect of aqueous extract of Crocus sativus L. (saffron) stigma against subacute effect of diazinon on specific biomarkers in rats.
In this study, the effect of aqueous extract of Crocus sativus L. (saffron) stigma was studied against subacute toxicity of diazinon (DZN) on specific biochemical markers in rats. Vitamin E (200 IU/kg) and the aqueous extract of saffron at doses 50, 100 and 200 mg/kg were injected intraperitoneally three times per week alone or with DZN (20 mg/kg/day, orally) for 4 weeks. Red blood cell (RBC) cholinesterase activity was inhibited by DZN and this effect was not affected by vitamin E or saffron plus DZN. The levels of serum tumor necrosis factor-α (inflammation marker), direct 8-iso-prostaglandin F(2α) (oxidative stress marker) and soluble protein-100 β (S100β, neuronal damage marker) were increased significantly by DZN. The saffron extract inhibited the effect of DZN on these biomarkers levels. However, vitamin E was able to only reduce 8-iso-prostaglandin F(2α) and S100β levels. This study showed that the aqueous extract of saffron prevents DZN-induced rise of several specific inflammation, oxidative stress and neuronal damage biomarkers.
1Medical Toxicology Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Islamic Republic of Iran.
Seyed Adel
SA
Moallem
Alireza Timcheh
AT
Hariri
Mahmoud
M
Mahmoudi
Hossein
H
Hosseinzadeh
D000818:Animals; D015415:Biomarkers / Q000097:blood*; D027622:Crocus / Q000737:chemistry*; D003976:Diazinon / Q000009:adverse effects*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D007249:Inflammation / Q000188:drug therapy / Q000209:etiology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D020258:Neurotoxicity Syndromes / Q000188:drug therapy / Q000209:etiology; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit / Q000097:blood; D059551:Toxicity Tests, Subacute; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014810:Vitamin E / Q000494:pharmacology
D015415:Biomarkers; D010936:Plant Extracts; D064568:S100 Calcium Binding Protein beta Subunit; C579158:S100b protein, rat; D014409:Tumor Necrosis Factor-alpha; D014810:Vitamin E; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D003976:Diazinon