Profiling plasma peptides for the identification of potential ageing biomarkers in Chinese Han adults.
Advancing age is associated with cardiovascular disease, diabetes mellitus and cancer, and shows significant inter-individual variability. To identify ageing-related biomarkers we performed a proteomic analysis on 1890 Chinese Han individuals, 1136 males and 754 females, aged 18 to 82 years, using weak cation exchange magnetic bead based MALDI-TOF-MS analysis. The study identified 44 peptides which varied in concentration in different age groups. In particular, apolipoprotein A-I (ApoA1) concentration gradually increased between 18 to 50 years of age, the levels of fibrinogen alpha (FGA) decreased over the same age span, while albumin (ALB) was significantly degraded in middle-aged individuals. In addition, the plasma peptide profiles of FGA and four other unidentified proteins were found to be gender-dependent. Plasma proteins such as FGA, ALB and ApoA1 are significantly correlated with age in the Chinese Han population and could be employed as indicative ageing-related biomarkers.
School of Public Health and Family Medicine, Capital Medical University, Beijing, People's Republic of China.
Jiapeng
J
Lu
Yuqing
Y
Huang
Youxin
Y
Wang
Yan
Y
Li
Yujun
Y
Zhang
Jingjing
J
Wu
Feifei
F
Zhao
Shijiao
S
Meng
Xinwei
X
Yu
Qingwei
Q
Ma
Manshu
M
Song
Naibai
N
Chang
Alan H
AH
Bittles
Wei
W
Wang
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D016632:Apolipoprotein A-I / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D005260:Female; D005340:Fibrinogen / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000097:blood*; D012709:Serum Albumin / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D059467:Transcriptome
D016632:Apolipoprotein A-I; D015415:Biomarkers; D010455:Peptides; D012709:Serum Albumin; C464794:fibrinogen Aalpha; D005340:Fibrinogen