Transcriptome-wide survey and expression analysis of stress-responsive NAC genes in Chrysanthemum lavandulifolium.
The plant-specific NAC (NAM, ATAF, and CUC) transcription factor family plays a vital role in various plant growth and developmental processes as well as in stress resistance. Using RNA sequencing, we found that the ClNAC genes (ClNAC1-44) were the most strongly up-regulated transcription factor family in Chrysanthemum lavandulifolium leaves under salt treatment. We carried out reverse transcriptase polymerase chain reaction to monitor ClNAC genes response against multiple stresses and hormonal treatments including salt, drought, cold, heat, abscisic acid and salicylic acid treatments. The results showed that 35 ClNAC genes were differentially expressed in different organ, and 32 ClNAC genes could respond to at least 2 kinds of treatments. Quantitative real time polymerase chain reaction showed that 10 ClNAC genes belonging to 7 different subfamilies could respond to at least 5 kinds of treatments. Over 50-fold variation in transcriptional levels of ClNAC17 and ClNAC21 genes was observed under 6 different types of treatments. In the present study, high-level expression of ClNAC genes under abiotic stresses and hormonal treatments suggests that the NAC transcription factors play important roles in abiotic stress tolerance and adaptation.
College of Landscape Architecture, Beijing Forestry University, Beijing 100038, China.
He
H
Huang
College of Landscape Architecture, Beijing Forestry University, Beijing 100038, China.
Yi
Y
Wang
College of Landscape Architecture, Beijing Forestry University, Beijing 100038, China; College of Life Science, Beijing Forestry University, Beijing 100038, China.
Shunli
S
Wang
College of Foresty, Beijing Forestry University, Beijing 100038, China.
Xuan
X
Wu
College of Landscape Architecture, Beijing Forestry University, Beijing 100038, China; School of Forestry and Environmental Studies, Yale University, New Haven, CT 06511, USA.
Ke
K
Yang
College of Landscape Architecture, Beijing Forestry University, Beijing 100038, China.
Yajing
Y
Niu
College of Landscape Architecture, Beijing Forestry University, Beijing 100038, China. Electronic address: silandai@sina.com.
Silan
S
Dai
D000040:Abscisic Acid / Q000378:metabolism; D000222:Adaptation, Physiological / Q000235:genetics*; D031202:Chrysanthemum / Q000235:genetics* / Q000502:physiology; D058639:Cold-Shock Response / Q000235:genetics; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000502:physiology*; D018869:Heat-Shock Response / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics*; D020156:Salicylic Acid / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors; D059467:Transcriptome
D014157:Transcription Factors; D012965:Sodium Chloride; D000040:Abscisic Acid; D020156:Salicylic Acid