The contribution of age structure to cell population responses to targeted therapeutics.
Cells grown in culture act as a model system for analyzing the effects of anticancer compounds, which may affect cell behavior in a cell cycle position-dependent manner. Cell synchronization techniques have been generally employed to minimize the variation in cell cycle position. However, synchronization techniques are cumbersome and imprecise and the agents used to synchronize the cells potentially have other unknown effects on the cells. An alternative approach is to determine the age structure in the population and account for the cell cycle positional effects post hoc. Here we provide a formalism to use quantifiable lifespans from live cell microscopy experiments to parameterize an age-structured model of cell population response.
UMR 7598 LJLL, BC187, Université Pierre et Marie Curie-Paris 6, 4 Place de Jussieu, F-75252 Paris Cedex 5, France. gabriel@ann.jussieu.fr
Pierre
P
Gabriel
Shawn P
SP
Garbett
Vito
V
Quaranta
Darren R
DR
Tyson
Glenn F
GF
Webb
D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002453:Cell Cycle / Q000187:drug effects*; D016922:Cellular Senescence / Q000187:drug effects*; D006801:Humans; D008954:Models, Biological; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology
D000970:Antineoplastic Agents