[Expression and significance of MCM2, Ki-67 and Rb protein in laryngeal squamous cell carcinomas].
OBJECTIVE
To investigate the expression of MCM2, Ki-67 and Rb and its biological characteristic in human laryngeal squamous cell carcinomas(LSCC).


METHODS
The expression of MCM2 protein and Rb protein were detected in 60 cases of LSCC, 10 cases of precarcinoma, 10 cases of vocal cord polyps and 10 cases of normal laryngeal tissues, and Ki-67 protein were detected in 60 cases of LSCC and 10 cases of normal laryngeal tissues by Elivision plus immunohistochemical staining, and analyze their relations with clinicopathological characteristics.


RESULTS
The positive expression rate of MCM2 in LSCC was significantly higher than that in precarcinoma and normal laryngeal tissues (P < 0.05), and was positively correlated with pathological grades, clinical stages and lymph node metastases (P < 0.05) of LSCC. The positive expression rate of Rb protein in LSCC was significantly lower than that in precarcinoma and normal laryngeal tissues (P < 0.05). The expression level of MCM2 in LSCC was negatively corelated with Rb (r = -0.542, P < 0.05), the expression level of Ki-67 in LSCC (76.67%) was significantly higher than that in normal laryngeal tissues (30.00%) (P < 0.01) and the expression level of MCM2 in LSCC was positively corelated with Ki-67(r = 0.596, P < 0.01). The LI of MCM2 in the 3-year survival rate of LSCC was significantly lower than that in Ki-67 (P < 0.05).


CONCLUSIONS
Over expression of MCM2 and loss of Rb protein were related to the carcinogenesis and development of LSCC. The determination of MCM2 can be an index for estimating the level of malignancy and prognosis of LSCC.
Department of Otorhinolaryngology-Head and Neck Surgery, People's Hospital of Guangzhou Huadu District, Guangzhou 510800, China.
Kaigui
K
Cai
Ying
Y
Luo
Lixiang
L
Li
Yuehui
Y
Liu
D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018797:Cell Cycle Proteins; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D007830:Larynx / Q000378:metabolism; D008207:Lymphatic Metastasis; D064111:Minichromosome Maintenance Complex Component 2 / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D011127:Polyps / Q000378:metabolism; D011230:Precancerous Conditions / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D016160:Retinoblastoma Protein / Q000378:metabolism*; D015996:Survival Rate; D014827:Vocal Cords / Q000378:metabolism
D018797:Cell Cycle Proteins; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D016160:Retinoblastoma Protein; C084859:MCM2 protein, human; D064111:Minichromosome Maintenance Complex Component 2