Ultrasensitive one-step rapid visual detection of bisphenol A in water samples by label-free aptasensor.
A simple, one-step, rapid method to detect bisphenol A (BPA) using a label-free aptasensor is presented. A high selective anti-BPA aptamer was added to gold nanoparticles (GNPs) to prepare the label-free aptasensor for BPA, which maintains good tolerance of GNPs under aqueous conditions with high salt concentrations. With the presence of BPA in the aptasensor system, the GNPs would aggregate by competitive binding of BPA and aptamer. Detection results can be visualized by the aggregation-induced color change of GNPs without the use of any instrumentation. The limit of visual detection (LOD) was found to be 0.1ng/mL by naked-eye observation, which was competitive to some current rapid BPA detection methods, even some instrumental based methods. Besides the obvious advantages, including reduced detection time and operation procedures, the results of this method meet the various detection requirements for BPA and are comparable to the traditional ELISA and instrument-based methods. The proposed one-step, label-free method was successfully used to determine BPA in actual water samples.
School of Biotechnology & Food Engineering, Key Lab of Food Nutrition & Safety of Anhui Province, Hefei University of Technology, Hefei 230009, PR China.
Zhanlong
Z
Mei
Huaqin
H
Chu
Wei
W
Chen
Feng
F
Xue
Jian
J
Liu
Huaneng
H
Xu
Rui
R
Zhang
Lei
L
Zheng
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000032:analysis*; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D013997:Time Factors; D014867:Water / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D006046:Gold; D010636:Phenols; D014867:Water; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds