Vitamin D-1α-hydroxylase and vitamin D-24-hydroxylase mRNA studies in chickens.
A series of experiments were conducted to study the basal amounts of vitamin D-1α-hydroxylase and vitamin D-24-hydroxylase mRNA amounts in different organs and the effect of immune stimulation on 1α- and 24-hydroxylase mRNA amounts in chickens. At day of hatch, kidneys had an approximately 66-fold higher amount of 1α-hydroxylase and 550-fold higher amount of 24-hydroxylase mRNA, thigh and breast muscles had an approximately 20-fold higher amount of 1α-hydroxylase mRNA, and the thymus had an approximately 41-fold higher amount of 24-hydroxylase mRNA than the liver. An in vivo LPS injection did not alter the amount of 1α-hydroxylase mRNA in the breast muscle (P=0.60) or in the kidneys (P=0.39). An in vivo LPS injection decreased (P=0.01) the amount of 24-hydroxylase mRNA in the breast muscle at 3 d post-LPS injection. An in vivo LPS injection increased (P=0.01) the amount of 24-hydroxylase mRNA in the kidneys at 2, 3, and 6 d post-LPS injection. An in vitro stimulation altered amounts of 1α- (P=0.01) and 24-hydroxylase (P=0.04) mRNA in CD4+ cells. In conclusion, the distribution of 1α- and 24-hydroxylase mRNA amounts was similar to mammals, and an immune stimulation altered the amounts of 1α- and 24-hydroxylase mRNA in chickens.
Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA.
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R
Shanmugasundaram
R K
RK
Selvaraj
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000201:enzymology; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007668:Kidney / Q000166:cytology / Q000201:enzymology; D008070:Lipopolysaccharides / Q000276:immunology; D008099:Liver / Q000201:enzymology; D008221:Lymphoid Tissue / Q000201:enzymology; D018482:Muscle, Skeletal / Q000201:enzymology; D011201:Poultry Diseases / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012486:Salmonella typhimurium; D013250:Steroid Hydroxylases / Q000235:genetics / Q000378:metabolism*; D065668:Vitamin D3 24-Hydroxylase
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D013250:Steroid Hydroxylases; C406322:vitamin D 1-alpha hydroxylase; D065668:Vitamin D3 24-Hydroxylase