[Progression on genetic knockout tools in Mycobacterium].
Pathogenic mycobacteria were and remain a heavy burden to public health. Unfortunately, genetic manipulation including knockout technologies of Mycobacterium is difficult compared with other traditional model organisms. To overcome this obstacle, achievements in Mycobacterium knockout technologies were summarized, including delivery vector, sequence-specific recombination system, as well as the recently developed recombinogenic engineering and its application. The future  for this tool innovation is also addressed.
Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, School of Life Sciences, Southwest University, Chongqing 400715, China. duql@swu.edu.cn
Qin-Lin
QL
Du
Xiang-Yu
XY
Fan
Jin-Xiao
JX
Mao
Jian-Ping
JP
Xie
D055786:Gene Knockout Techniques; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D016296:Mutagenesis; D009161:Mycobacterium / Q000235:genetics*; D011995:Recombination, Genetic