[Effects of mesangial cell on proliferation and function of osteoblast in vitro].
OBJECTIVE
To explore the effects and mechanism of mesangial cell (MC) on proliferation and function of osteoblast in vitro from growth factors.


METHODS
Osteoblast cultured in vitro were divided into normal blood serum group and MC group and cultured supernatant fluid by 10% serum culture medium and 20% MC culture medium. MTF was used to checked the proliferation of osteoblast at the end of 24, 48, 72, 120 h; Expression of BGP and OPN were tested 72 h and 144 h after culture; Expression of IGF-alpha and TGF-beta were tested 144 h after culture.


RESULTS
The proliferation of osteoblast in MC group was obviously higher than normal blood serum group in different times (P < 0.05); The level of BGP and OPN of osteoblast was obviously higher than normal blood serum group (11.3%, 16.4%, 55.0% and 39.6%); and the level of IGF-alpha and TGF-beta of osteoblast was obviously higher than normal blood serum group (10.1% and 47.7%).


CONCLUSIONS
MC can directly act on osteoblast, promote the proliferation and function of osteoblast by promoting secretion of TGF-beta.
Nephrology Department of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China.
Feng
F
Wu
Ri-yang
RY
Lin
Li-qun
LQ
He
D000818:Animals; D049109:Cell Proliferation; D008297:Male; D050527:Mesangial Cells / Q000502:physiology*; D010006:Osteoblasts / Q000502:physiology*; D053495:Osteopontin / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000502:physiology
D016212:Transforming Growth Factor beta; D053495:Osteopontin