[Dynamic expressions of IL-17 and IL-23 in mice infected with Schistosoma japonicum].
OBJECTIVE
To investigate the dynamic expressions of IL-17 and IL-23 in mice with Schistosoma japonicum infection.


METHODS
A murine model of the infection of S. japonicum was established. The suspension of spleen cells incubated with ConA was collected at 0, 1, 2, 4, 6, 8 and 10 weeks post-infection. Sandwich ELISA and RT-PCR were used to measure the expressions of IL-17A and IL-23p19 on protein and mRNA level.


RESULTS
The dynamic changes of IL-17A and IL-23p19 showed a positive correlation. The level of IL-17A increased and reached the peak at 1 week after the infection, reduced at 4 weeks after the infection, and was even lower at 8 weeks post-infection. The level of IL-17 mRNA increased at 1 week post-infection, and then decreased gradually at 2 weeks post-infection. Being consistent with the dynamic expression of IL-17A, the IL-23p19 expression increased at 1 week post-infection, went up to the peak at 2 weeks post-infection, and gradually reduced into the normal level at 4 weeks. However, the expression of IL-23p19 mRNA fluctuated in the normal range, increased at 4 weeks post-infection, and reached the peak at 6 weeks post-infection.


CONCLUSIONS
IL-17 and IL-23 are of co-expression in the mice after schistosome infection. There is a significant increase in the early stage of the infection. IL-17 and IL-23 may play important roles during the immune process in the very early stage of Schistosoma infection.
Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Bin-Tao
BT
Su
Hong
H
Xu
Fei
F
Guan
Lin
L
Chen
Yan
Y
Zhou
Jia-Hui
JH
Lei
Wen-Qi
WQ
Liu
D000818:Animals; D020381:Interleukin-17 / Q000378:metabolism*; D053759:Interleukin-23 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism
D020381:Interleukin-17; D053759:Interleukin-23; D012333:RNA, Messenger