A role for antioxidants in acclimation of marine derived pathogenic fungus (NIOCC 1) to salt stress.
Salinity tolerance a key factor helps in understanding the ionic homeostasis in general, which is a fundamental cellular phenomenon in all living cells. Here, a marine derived pathogenic fungus was examined for its adaptation under salt stress using antioxidant properties. The aqueous extracts of halophilic fungus exhibited different levels of antioxidant activity in all the in vitro tests such as α,α-diphenyl-β-picrylhydrazyl (DPPH(·)), Hydroxyl Radical Scavenging Assay (HRSA), Metal chelating assay and β-carotene-linoleic acid model system. The antioxidant capacity of marine fungus exposed to high salt condition showed an increase in activity. In addition, the production of intra and extracellular antioxidant enzymes of the fungus at various salt stresses were analyzed and discussed for their possible role in the stress mechanism. The marine derived fungus was identified as Phialosimplex genus, which is associated with infections in dogs. Thus the present study elucidates that the scavenging activity is one of the protective mechanisms developed in the fungus to avoid the deleterious effect of salt stress. In addition, the study also helps in understanding how the pathogenic fungus tackles the oxidative burst i.e. hypersensitivity reaction performed by host to kill the pathogens.
Biotechnology Laboratory, Biological Oceanography Division, National Institute of Oceanography, Council of Scientific and Industrial Research, Dona Paula, Goa 403004, India. cravindran@nio.org
Chinnarajan
C
Ravindran
Govindaswamy R
GR
Varatharajan
Raju
R
Rajasabapathy
S
S
Vijayakanth
Alagu Harish
AH
Kumar
Ram M
RM
Meena
D000975:Antioxidants / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005658:Fungi / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction; D012623:Seawater / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism*
D000975:Antioxidants; D005656:Fungal Proteins; D012965:Sodium Chloride