A lateral flow biosensor for detection of single nucleotide polymorphism by circular strand displacement reaction.
A lateral flow biosensor for detection of single nucleotide polymorphism based on circular strand displacement reaction (CSDPR) has been developed. Taking advantage of high fidelity of T4 DNA ligase, signal amplification by CSDPR, and the optical properties of gold nanoparticles, this assay has reached a detection limit of 0.01 fM.
South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China.
Zhuo
Z
Xiao
Puchang
P
Lie
Zhiyuan
Z
Fang
Luxin
L
Yu
Junhua
J
Chen
Jie
J
Liu
Chenchen
C
Ge
Xuemeng
X
Zhou
Lingwen
L
Zeng
D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D011088:DNA Ligases / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques; D020641:Polymorphism, Single Nucleotide
D006046:Gold; D004247:DNA; D011088:DNA Ligases