Thin phosphatidylcholine films as background surfaces with further possibilities of functionalization for biomedical applications.
Non-specific adsorption is a crucial problem in the biomedical field. To produce surfaces avoiding this phenomenon, we functionalized thin (7-180 nm) poly(methylhydrosiloxane) (PMHS) network films at room temperature (≈20°C) with phospholipids (PL) bearing a phosphorylcholine head. Regardless of their mode of preparation (casting or immersion), all surfaces appeared to be very hydrophilic with a captive air-bubble contact angle stabilized around 40°. The thin films were protein-repellent in phosphate saline buffer pH 7.4 according to analysis by normal scanning confocal fluorescence. Neither was any adsorption or spreading of l-α-phosphatidylcholine liposomes on such films observed. In addition, amino functional groups could be easily attached to the surface remaining available for further functionalization.
Institut Européen des Membranes, Université Montpellier 2 (ENSCM, UM2, CNRS), Montpellier, France.
Lara
L
Tauk
Thierry
T
Thami
Lynda
L
Ferez
Armagan
A
Kocer
Jean-Marc
JM
Janot
Philippe
P
Déjardin
D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties
D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D010743:Phospholipids; D011506:Proteins