Generation of monoclonal antibodies to recombinant vascular endothelial growth factor.
Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF.
Department of Medical Nanobiotechnologies, N. I. Pirogov Russian National Research Medical University, Moscow, Russia. atomos@rambler.ru
S A
SA
Shein
O I
OI
Gurina
A V
AV
Leopol'd
V P
VP
Baklaushev
A A
AA
Korchagina
N F
NF
Grinenko
N V
NV
Ivanova
N E
NE
Volgina
I A
IA
Ryabukhin
V P
VP
Chekhonin
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D042461:Vascular Endothelial Growth Factor A / Q000276:immunology*
D000911:Antibodies, Monoclonal; D042461:Vascular Endothelial Growth Factor A