Spatio-temporal analysis of cyprinid herpesvirus 3 genetic diversity at a local scale.
Cyprinid herpesvirus 3 (CyHV-3), the causative agent of koi herpesvirus disease, is a major threat for carp populations in many countries worldwide, including Indonesia. It has been shown that many genotypes circulate worldwide, all highly related to one of the two known lineages U/I and J. In this study, we evaluated the spatial and temporal distribution of CyHV-3 strains in a small enzootic area, the lake of Cirata (West Java, Indonesia). Of the 365 samples analysed, from clinical or asymptomatic fish, 244 were found positive for CyHV-3, suggesting a high occurrence of the virus. Genotyping of these viral specimens with a range of molecular markers revealed the presence of numerous haplotypes in the host population, all related to the J lineage. In single individuals, mixed-genotype infections occurred at high frequency. The present results demonstrate that polymorphic molecular markers are suitable to monitor the genetic evolution of a viral population in an enzootic area.
Institut des Sciences de l'Evolution de Montpellier, UMR226 IRD-CNRS-UM2, Montpellier, France. jean-christophe.avarre@ird.fr
J-C
JC
Avarre
A
A
Santika
A
A
Bentenni
Z
Z
Zainun
J-P
JP
Madeira
M
M
Maskur
L
L
Bigarré
D
D
Caruso
D000818:Animals; D002347:Carps / Q000821:virology*; D005393:Fish Diseases / Q000821:virology*; D014644:Genetic Variation; D005838:Genotype; D005880:Gills / Q000821:virology; D006564:Herpesviridae / Q000145:classification / Q000235:genetics*; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D007214:Indonesia; D018598:Minisatellite Repeats / Q000235:genetics; D010802:Phylogeny