Functionalized Fe₃O₄@Au superparamagnetic nanoparticles: in vitro bioactivity.
The interaction of nanoparticles with cells has been a focus of interest during the past decade. We report the fabrication and characterization of hydrosoluble Fe₃O₄@Au nanoparticles functionalized with biocompatible and fluorescent molecules and their interaction with cell cultures by visualizing them with confocal microscopy. Gold covered iron oxide nanoparticles were synthesized by reducing metal salts in the presence of oleylamine and oleic acid. The functionalization of these particles with an amphiphilic polymer provides a water soluble corona as well as the possibility to incorporate different molecules relevant for bio-applications such as poly(ethylene glycol), glucose or a cadaverine derived dye. The particle size, and the presence of polymer layers and conjugated molecules were characterized and confirmed by transmission electron microscopy, thermogravimetric measurements and infrared spectroscopy. A complete magnetic study was performed, showing that gold provides an optimum coating, which enhances the superparamagnetic behaviour observed above 10-15 K in this kind of nanoparticle. The interaction with cells and the cytotoxicity of the Fe₃O₄@Au preparations were determined upon incubation with the HeLa cell line. These nanoparticles showed no cytotoxicity when evaluated by the MTT assay and it was demonstrated that nanoparticles clearly interacted with the cells, showing a higher level of accumulation in the cells for glucose conjugated nanoparticles.
Departamento de Quíımica Inorgánica, Facultad de Ciencia y Tecnología, UPV/EHU, Apdo. 644, 48080 Bilbao, Spain.
J
J
Salado
M
M
Insausti
L
L
Lezama
I
I
Gil de Muro
M
M
Moros
B
B
Pelaz
V
V
Grazu
J M
JM
de la Fuente
T
T
Rojo
D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D060328:Magnetic Phenomena; D009682:Magnetic Resonance Spectroscopy; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013501:Surface-Active Agents / Q000737:chemistry; D013818:Thermogravimetry; D014867:Water / Q000737:chemistry
D020099:Coated Materials, Biocompatible; D005290:Ferric Compounds; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D013501:Surface-Active Agents; D014867:Water; C000499:ferric oxide; D011092:Polyethylene Glycols; D006046:Gold; D005947:Glucose