Altered expression of Krüppel-like factor 4 and β-catenin in human gastric cancer.
The effects of the interaction between KLF4 and β-catenin may be significant in human carcinogenesis and tumor development. This study aimed to determine whether the expression of KLF4 and β-catenin in gastric cancer tissues is associated with clinicopathological characteristics. Western blot analysis and immunohistochemistry were performed to detect KLF4 and β-catenin expression in tumor and corresponding non-cancerous tissues from 49 patients. The data revealed that KLF4 expression was significantly reduced in gastric cancer tissues compared with normal tissues. By contrast, the expression of the β-catenin protein was significantly increased in all tumor tissues, but was not expressed in distant normal mucosae. The altered expression of the KLF4 and β-catenin proteins was associated with advanced tumor stage and gastric cancer. In addition, the expression of the KLF4 and β-catenin proteins was inversely associated in moderately differentiated human gastric cancers. This study showed that β-catenin expression is significantly increased and KLF4 protein expression is markedly decreased in gastric cancer tissues, thus showing that the expression of KLF4 is inversely correlated with that of β-catenin in gastric cancer. The altered expression of the two proteins is associated with advanced tumor stage in gastric cancer.
Department of General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
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Zhang
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Zhang
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Wang
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Zha
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Huang