Jurkat cell proliferation is suppressed by Chlamydia (Chlamydophila) pneumoniae infection accompanied with attenuation of phosphorylation at Thr389 of host cellular p70S6K.
Chlamydia (Chlamydophila) pneumoniae infects T lymphocytes and multiplies within them. Our previous studies have indicated that C. pneumoniae infection suppresses proliferation of peripheral blood mononuclear cells stimulated with Staphylococcus-enterotoxin B; however, the mechanism of suppression was unclear. In this study, we explored the molecular mechanism involved in C. pneumoniae infection by using human acute T cell leukemia cell line, Jurkat E6-1. Proliferation of Jurkat cells was suppressed in an m.o.i.-dependent manner by C. pneumoniae infection. The suppression by the infection was particularly evident during the initial 24h of the infection, and down modulation of cyclin D3 protein levels were observed at the same time period by immunoblot analysis. The suppression of the Jurkat cell proliferation and the down modulation of cyclin D3 protein level were only induced by viable C. pneumoniae infection, not by exposure to UV-killed or heat-killed C. pneumoniae. Phosphorylations at Thr308 and Ser473 of AKT were induced by C. pneumoniae infection; however, phosphorylation at Thr389 of the downstream kinase, p70S6K was inhibited by unidentified mechanism associated with C. pneumoniae infection. Taking into account that G1 arrest of the C. pneumoniae infected Jurkat cells were not observed and that p70S6K is one of the most important regulators of protein synthesis, it was suggested that the suppression of Jurkat cell proliferation by C. pneumoniae was at least in part mediated by down modulation of protein synthesis through attenuation of Thr389 phosphorylation of p70S6K.
Department of Biomedical Informatics, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan. hiraii@glocol.osaka-u.ac.jp
Itaru
I
Hirai
Megumi
M
Ebara
Shoko
S
Nakanishi
Chihiro
C
Yamamoto
Tadahiro
T
Sasaki
Kazuyoshi
K
Ikuta
Yoshimasa
Y
Yamamoto
D061387:Chlamydial Pneumonia / Q000201:enzymology / Q000276:immunology*; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D059585:G1 Phase Cell Cycle Checkpoints / Q000276:immunology*; D006801:Humans; D019169:Jurkat Cells; D010766:Phosphorylation / Q000276:immunology; D014176:Protein Biosynthesis / Q000276:immunology*; D051057:Proto-Oncogene Proteins c-akt / Q000276:immunology* / Q000378:metabolism; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000276:immunology* / Q000378:metabolism; D013912:Threonine / Q000276:immunology / Q000378:metabolism
D013912:Threonine; D051057:Proto-Oncogene Proteins c-akt; D038762:Ribosomal Protein S6 Kinases, 70-kDa