Homogeneous assay for evaluation of aptamer-protein interaction.
We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the K(D) value of aptamer-protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer-target protein interactions during aptamer selection.
Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, H-1111 Budapest, Hungary.
Gergely
G
Lautner
Zsófia
Z
Balogh
Anna
A
Gyurkovics
Róbert E
RE
Gyurcsányi
Tamás
T
Mészáros
D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism*; D036022:Capsid Proteins / Q000737:chemistry / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry; D007700:Kinetics; D009842:Oligopeptides / Q000737:chemistry; D011485:Protein Binding; D020349:Surface Plasmon Resonance
D052157:Aptamers, Nucleotide; D036022:Capsid Proteins; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D006639:Histidine