Triptolide inhibits proliferation and induces apoptosis of human melanoma A375 cells.
Triptolide, a diterpenoid obtained from Tripteryglum wilfordii Hook.f, has attracted interest for its anti- tumor activities against human tumor cell lines in recent years. This report focuses on anti-proliferative and pro-apoptotic activities in human melanoma A375 cells assessed by CCK8 assay, Hoechst 33258 staining and flow cytometry. In addition, triptolide-induced arrest in the S phase was also observed. Caspase assays showed the apoptosis induced by triptolide was caspase-dependent and probably through intrinsic apoptotic pathways. Furthermore, expression of NF-κB (p65) and its downstream factors such as Bcl-2, Bcl-XL was down-regulated. Taken together, the data indicate that triptolide inhibits A375 cells proliferation and induces apoptosis by a caspase-dependent pathway and through a NF-κB-mediated mechanism.
Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, China.
Yue
Y
Tao
Meng-Li
ML
Zhang
Peng-Cheng
PC
Ma
Jian-Fang
JF
Sun
Wu-Qing
WQ
Zhou
Yu-Ping
YP
Cao
Ling-Jun
LJ
Li
D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D004852:Epoxy Compounds / Q000494:pharmacology; D006801:Humans; D008545:Melanoma / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D010616:Phenanthrenes / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects / Q000378:metabolism; D059807:S Phase Cell Cycle Checkpoints / Q000187:drug effects; D012878:Skin Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D051020:bcl-X Protein / Q000187:drug effects / Q000378:metabolism
D018906:Antineoplastic Agents, Alkylating; D004224:Diterpenes; D004852:Epoxy Compounds; D016328:NF-kappa B; D010616:Phenanthrenes; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein; C001899:triptolide; D053148:Caspase 3; D053453:Caspase 9