[Adsorptive immobilization of rhodococcal cells in hydrophobized derivatives of wide-pore polyacrylamide cryogel].
Adsorption of Rhodococcus ruber cells on columns with polyacrylamide cryogel (CryoPAAG) partially hydrophobized by different quantities (0.2, 1, and 5 mol %) of chemically grafted n-dodecane residues has been studied. The adsorption capacity (1.1 x 10(9) cells/g) of gel carrier for rhodococcal cells and the optimal content (1 mol %) of hydrophobizing groups were determined. The respirometric method showed the high catalytic activity and functional stability of immobilized bacterial cells. Respiratory activity of immobilized rhodococci in the presence of a model mixture of oil hydrocarbons exceeded the respective parameter for free cells by 12-17%. Viability of rhodococcal cells adsorptionally fixed in hydrophobized cryoPAAG was maintained at a level of 93-95% after a half-year period of storage. The results may be used for development of immobilized biocatalyst for directed transformation of hydrocarbon compounds and biological purification of oil-polluted water.
M S
MS
Kuyukina
I B
IB
Ivshina
E V
EV
Rubtsova
R V
RV
Ivanov
V I
VI
Lozinskiĭ
D000180:Acrylic Resins / Q000737:chemistry*; D000332:Aerobiosis / Q000502:physiology*; D000473:Alkanes / Q000737:chemistry; D001422:Bacterial Adhesion; D055162:Biocatalysis; D018914:Cells, Immobilized; D059985:Cryogels / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007219:Industrial Oils; D050296:Microbial Viability; D009821:Oils / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D016062:Porosity; D012240:Rhodococcus / Q000737:chemistry / Q000502:physiology*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000473:Alkanes; D059985:Cryogels; D009821:Oils; D010100:Oxygen; D000099166:Polyacrylamides; C007548:n-dodecane