Investigation of biological activities of dichloromethane and ethyl acetate fractions of Platonia insignis Mart. seed.
Platonia insignis Mart., a native species of the Brazilian Amazon more commonly known as bacuri, is a member of the Clusiaceae family. In this study, we evaluated the chemical composition and the antioxidant and toxicity activities of the dichloromethane and ethyl acetate fractions from P. insignis seed ethanolic extract using different experimental models. Our results demonstrate in vitro antioxidant effects, by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt and 1,1-diphenyl-2-picryl-hydrazyl assays, as well as in vivo effects in antioxidant-defective Saccharomyces cerevisiae strains to both fractions. Toxicity was evaluated against the micro-crustaceous Artemia salina Leach. and promastigote Leishmania amazonensis. The dichloromethane fraction was the most active fraction evaluated on A. salina and promastigote L. amazonensis (IC(50)  = 24.89 μg/mL and 2.84 μg/mL, respectively). In addition, a slight cytotoxicity was observed in mammalian V79 cells using ethyl acetate and dichloromethane fractions with MTT assays. Both fractions displayed genotoxicity up to 25 μg/mL (dichloromethane) and 10 μg/mL (ethyl acetate) in V79 cells, as evaluated by the alkaline comet assay. Thus, in this study, we demonstrate for the first time that ethyl acetate and dichloromethane fractions from P. insignis seeds display antioxidant effects, a toxic effect against A. salina and L. amazonensis and induce genotoxicity in V79 mammalian cells. The observed activities can be attributed to the phenolic compounds present in these fractions and to the presence of xanthones (alpha- and gamma-mangostin).
Post-Graduation Programme in Genetics and Applied Toxicology, Lutheran University of Brazil, Canoas, RS, Brazil.
Joaquim S
JS
Costa Júnior
Alexandre B F
AB
Ferraz
Taciana O
TO
Sousa
Romézio A C
RA
Silva
Sidney G
SG
De Lima
Chistiane M
CM
Feitosa
Antônia M G L
AM
Citó
Ana A C
AA
Melo Cavalcante
Rivelilson M
RM
Freitas
Angelo R
AR
Moura Sperotto
Valéria F
VF
Péres
Dinara J
DJ
Moura
Jenifer
J
Saffi
D000085:Acetates; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001156:Artemia / Q000187:drug effects; D028084:Clusiaceae / Q000737:chemistry; D006224:Cricetinae; D003412:Cricetulus; D007891:Leishmania / Q000187:drug effects; D008752:Methylene Chloride; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds
D000085:Acetates; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D008752:Methylene Chloride; C007650:ethyl acetate