Chemical proteomics and its impact on the drug discovery process.
Despite the rapid growth of postgenomic data and fast-paced technology advancement, drug discovery is still a lengthy and difficult process. More effective drug design requires a better understanding of the interaction between drug candidates and their targets/off-targets in various situations. The ability of chemical proteomics to integrate a multiplicity of disciplines enables the direct analysis of protein activities on a proteome-wide scale, which has enormous potential to facilitate drug target elucidation and lead drug verification. Over recent years, chemical proteomics has experienced rapid growth and provided a valuable method for drug target identification and inhibitor discovery. This review introduces basic concepts and technologies of different popular chemical proteomic approaches. It also covers the essential features and recent advances of each approach while underscoring their potentials in drug discovery and development.
The Biomedical Research Centre, University of British Columbia, Room #401, 2222 Health Sciences Mall, Vancouver, BC, V6T1Z3 Canada.
Qing
Q
Miao
Cheng-Cheng
CC
Zhang
Juergen
J
Kast
D057930:Click Chemistry / Q000379:methods; D055808:Drug Discovery / Q000379:methods*; D004798:Enzymes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry; D015335:Molecular Probes / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D054852:Small Molecule Libraries / Q000737:chemistry
D004798:Enzymes; D005456:Fluorescent Dyes; D015335:Molecular Probes; D011506:Proteins; D054852:Small Molecule Libraries