Dual effect of amino modified polystyrene nanoparticles on amyloid β protein fibrillation.
The fibrillation kinetics of the amyloid β peptide is analyzed in presence of cationic polystyrene nanoparticles of different size. The results highlight the importance of the ratio between the peptide and particle concentration. Depending on the specific ratio, the kinetic effects vary from acceleration of the fibrillation process by reducing the lag phase at low particle surface area in solution to inhibition of the fibrillation process at high particle surface area. The kinetic behavior can be explained if we assume a balance between two different pathways: first fibrillation of free monomer in solution and second nucleation and fibrillation promoted at the particle surface. The overall rate of fibrillation will depend on the interplay between these two pathways, and the predominance of one mechanism over the other will be determined by the relative equilibrium and rate constants.
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland. celiacl@uvigo.es
Celia
C
Cabaleiro-Lago
Fiona
F
Quinlan-Pluck
Iseult
I
Lynch
Kenneth A
KA
Dawson
Sara
S
Linse
D000682:Amyloid / Q000187:drug effects* / Q000648:ultrastructure; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000187:drug effects*; D052160:Benzothiazoles; D016503:Drug Delivery Systems; D005456:Fluorescent Dyes / Q000032:analysis; D005470:Fluorometry; D006801:Humans; D008854:Microscopy, Electron; D053758:Nanoparticles; D016624:Negative Staining; D009391:Nephelometry and Turbidimetry; D010446:Peptide Fragments / Q000737:chemistry / Q000187:drug effects*; D011137:Polystyrenes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D011994:Recombinant Proteins / Q000737:chemistry / Q000187:drug effects; D013499:Surface Properties; D013844:Thiazoles / Q000032:analysis
D000682:Amyloid; D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D011137:Polystyrenes; D011994:Recombinant Proteins; D013844:Thiazoles; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42); C009462:thioflavin T