[Simultaneous determination of 7 alkaloid in herba Sophorae Alopecuroidis by HPLC].
OBJECTIVE
To establish a method for determination of 7 alkaloid in Herba Sophorae Alopecuroidis by HPLC.


METHODS
X-Brige C18 (4.6 mm x 200 mm, 5 microm) column was used with acetonitriles-0.05 mol x L(-1) KH2PO4 solution (2.0 mL x L(-1) triethylamine) with gradient elution as the mobile phase and 1.0 mL x min(-1) as the flow rate. The detection wavelength was 205 nm.


RESULTS
Aloperin curve was linear in the range from 20.66 to 103.32 microg (r = 0.998 8) and the average recovery was 97.12% (RSD 7. 3%); sophoridine curve was linear in the range from 22.82 to 114.12 microg (r = 0.999 7) and the average recovery was 97.47% (RSD 3.0%); oxymatrine curve was linear in the range from 25.10 to 125.52 microg (r = 0.999 1) and the average recovery was 96.21% (RSD 4.5%); oxysophocarpine curve was linear in the range from 23.88 to 119.40 microg (r = 0.997 5) and the average recovery was 94. 64% (RSD 5.2%); matrine curve was linear in the range from 5.00 to 24.99 microg (r = 0.998 6) and the average recovery was 98.04% (RSD 5.4%); sophocarping curve was linear in the range from 4.69 to 23.46 microg (r = 0.999 6) and the average recovery was 96.24 (RSD 5.8%); lehmannine curve was linear in the range from 4.60 to 23.01 microg (r = 0.997 8) and the average recovery was 101.31% (RSD 4.3%).


CONCLUSIONS
The method is accurate, simple and feasible. It can be used as a quality evaluation in Herba S. Alopecuroidis.
Ningxia Institute of Drug Control, Yinchuan 750001, China.
Ling
L
Ma
Junqing
J
Wang
Jie
J
Tian
Kun
K
Wang
Wei
W
Li
Yinghua
Y
Wang
D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D010880:Piperidines / Q000032:analysis; D011786:Quality Control; D054837:Quinolizidines / Q000032:analysis; D011807:Quinolizines / Q000032:analysis; D029909:Sophora / Q000737:chemistry*; D000093842:Matrines
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D010880:Piperidines; D054837:Quinolizidines; D011807:Quinolizines; C533368:lehmannine; C062701:aloperine; C046246:oxysophocarpine; C037573:oxymatrine; D000093842:Matrines