Effects of emodin extracted from Chinese herbs on proliferation of non-small cell lung cancer and underlying mechanisms.
To aim of this was to observe emodin-mediated cytotoxicity and its influence on Rad51 and ERCC1 expressionin non-small cell lung cancer (NSCLC). NSCLC cells were cultured in vitro with emodin at various concentrations (0, 25, 50, 75 and 100 μmol/L) for 48 h and the proliferation inhibition rate was determined by the MTT method. Then, NSCLC were treated with emodin (SK-MES-1 40 μmol/L, A549 70 μmol/L) or 20 μmol/L U0126 (an ERK inhibitor) for 48 h, or with various concentrations of emodin for 48 h and the protein and mRNA expressions of ERCC1 and Rad51 were determined by RT-PCR and Western blot assay, respectively. Emodin exerted a suppressive effect on the proliferation of NSCLC in a concentration dependent manner. Protein and mRNA expression of ERCC1 and Rad51 was also significantly decreased with the dose. Vacuolar degeneration was observed in A549 and SK-MES-1 cell lines after emodin treatment by transmission electron microscopy. Emodin may thus inhibited cell proliferation in NSCLC cells by downregulation ERCC1 and Rad51.
Department of Pathology, Institute of Neuroscience, Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, China.
Lin
L
He
Juan-Juan
JJ
Bi
Qian
Q
Guo
Yin
Y
Yu
Xiu-Feng
XF
Ye
D000704:Analysis of Variance; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004642:Emodin / Q000494:pharmacology*; D004720:Endonucleases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051135:Rad51 Recombinase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000648:ultrastructure
D004268:DNA-Binding Proteins; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D051135:Rad51 Recombinase; C071447:ERCC1 protein, human; D004720:Endonucleases; D004642:Emodin