Haplotypes in the expression quantitative trait locus of interleukin-1β gene are associated with schizophrenia.
Recent genome-wide association study (GWAS) and gene expression analyses have revealed that single nucleotide polymorphisms (SNPs) associated with complex diseases such as schizophrenia are significantly more likely to be associated with expression quantitative trait loci (eQTL). The interleukin-1β (IL1B) gene has been strongly implicated in the susceptibility to schizophrenia. In order to test this association, we selected five tag SNPs in the eQTL of the IL1B gene and conducted a case-control study using two independent samples. The first sample comprised 528 schizophrenic patients and 709 controls and the second sample comprised 576 schizophrenic patients and 768 controls. We identified two SNPs and several haplotypes as being significantly associated with schizophrenia. Previous reports indicated that one major haplotype that was protective against schizophrenia reduced IL1B transcription, while two risk haplotypes for schizophrenia enhanced IL1B transcription. Therefore, we measured IL1B mRNA expression in PAXgene-stabilized whole blood from 40 schizophrenic patients and 40 controls to explore the possibility of using five tag SNPs as schizophrenic trait markers. A multiple regression analysis taking confounding factors into account revealed that the T allele of rs4848306 SNP, which is a protective allele for schizophrenia, predicted reduced change in IL1B mRNA expression, regardless of phenotype. Our results appear to support the previous hypothesis that IL1B contributes to the genetic risk of schizophrenia and warrant further research on the association of eQTL SNPs with schizophrenia.
Department of Psychiatry, Kobe University Graduate School of Medicine, Kobe, Japan.
Masakuni
M
Yoshida
Kyoichi
K
Shiroiwa
Kentaro
K
Mouri
Hiroki
H
Ishiguro
Irwan
I
Supriyanto
Woraphat
W
Ratta-Apha
Noriomi
N
Eguchi
Satoshi
S
Okazaki
Toru
T
Sasada
Masaaki
M
Fukutake
Takeshi
T
Hashimoto
Toshiya
T
Inada
Tadao
T
Arinami
Osamu
O
Shirakawa
Akitoyo
A
Hishimoto
D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D005260:Female; D005787:Gene Frequency; D055106:Genome-Wide Association Study; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D012559:Schizophrenia / Q000235:genetics*; D012737:Sex Factors
D053583:Interleukin-1beta; D012333:RNA, Messenger