Efficient establishment of pig embryonic fibroblast cell lines with conditional expression of the simian vacuolating virus 40 large T fragment.
The pig is an important animal for both agricultural and medical purposes. However, the number of pig-derived cell lines is relatively limited when compared with mouse- and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 40 large T fragment (SV40T) which allowed us to efficiently establish pig embryonic fibroblast cell lines. The established cell lines showed high levels of cell proliferation and resistance to cellular senescence. A chromosome analysis showed that 84% of the cells had the normal karyotype. Transient expression of the Cre recombinase allowed us to excise the SV40T fragment from the genome. The development of this research tool will enable us to quickly establish new cell lines derived from various animals.
Laboratory of Animal Genetics, Graduate School of Agricultural Sciences, Tohoku University, Sendai, Japan. tomofukuda009@gmail.com
Tomokazu
T
Fukuda
Masafumi
M
Katayama
Takayuki
T
Yoshizawa
Takahiro
T
Eitsuka
Hiroaki
H
Mizukami
Kiyotaka
K
Nakagawa
Hisao
H
Ito
Homika
H
Komagata
Sanghoun
S
Song
Sanggun
S
Roh
Yumi
Y
Hoshino
Eimei
E
Sato
Hirofumi
H
Hanada
Katsuhiko
K
Nishimori
Teruo
T
Miyazawa
Takafumi
T
Uchida
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002460:Cell Line; D049109:Cell Proliferation; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000821:virology; D018703:Founder Effect; D015870:Gene Expression; D005818:Genetic Engineering; D019426:Integrases / Q000235:genetics; D059785:Karyotype; D007621:Karyotyping; D013539:Simian virus 40 / Q000235:genetics*; D013552:Swine
D000952:Antigens, Polyomavirus Transforming; C045073:Cre recombinase; D019426:Integrases