Development of estimation methods of skin oxidation and evaluation of anti-oxidative effects of genistein in topical formulations.
The objective of the present study was to establish the method of measurement of hydrogen peroxide and to estimate the anti-oxidative effect of genistein in the skin. UVB induced skin oxidation and anti-oxidative effect of genistein formulations were evaluated by determining levels of hydrogen peroxide. The mechanism involved in the determination of hydrogen peroxide is based on a color reaction between ferric ion (Fe(3+)) and xylenol orange, often called FOX assay and subsequent monitoring of absorbance values of the reactant at 540 nm. The reaction was to some extent pH-dependent and detection sensitivity was greatest at pH 1.75. Genistein liposomal gel demonstrated better anti-oxidative effect with regard to lowering hydrogen peroxide levels elevated by UVB irradiation compared to genistein-suspended gel. A linear relationship has been observed between anti-oxidative effect of genistein and drug deposition in the skin tissue. Genistein liposomal gel resulting in the localization of the drug in the deeper skin led to improved anti-oxidative effect compared to genistein gel. The suggested method for evaluation of oxidation of the skin can be used as a tool to screen effective anti-oxidative agents and their delivery systems acting on the skin.
College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
Seong Yeon
SY
Kim
Yeon Joo
YJ
Na
Dongju
D
Kim
Yeongseok
Y
Kim
Hyeong Min
HM
Kim
Sung-Ha
SH
Hwang
Jiyeon
J
Kwak
Hyo-Jeong
HJ
Kuh
Jaehwi
J
Lee