Oxidative damage, inflammation, and Toll-like receptor 4 pathway are increased in preeclamptic patients: a case-control study.
OBJECTIVE
There was no direct correlation between plasma and placental oxidative damage parameters and inflammation and evidence of TLR4 pathway activation in the placenta in preeclamptic (PE) patients.


METHODS
33 PE patients and 33 normotensive pregnant women were included. The maternal section of the placenta and blood were collected to the determination of oxidative damage markers (thiobarbituric acid reactive species and protein carbonyls), inflammatory response (interleukin-6 and myeloperoxidase activity), and activation of the TLR-4-NF-kB pathway.


RESULTS
An increase of IL-6 levels in both plasma and placenta was observed, but myeloperoxidase activity was not significantly different comparing the groups. Oxidative damage parameters were increased in plasma and placenta in PE patients. A significant increase of the protein levels of TLR-4 and NF-kB was observed in the placenta.


CONCLUSIONS
The TLR4-NF-kB pathway is upregulated in PE, probably generating local and systemic inflammatory response that is followed by local and systemic oxidative damage.
Laboratório de Fisiopatologia Experimental and Instituto Nacional Translacional em Medicina (INCT-TM), Universidade do Extremo Sul Catarinense, Avenida Universitária, 1105, 88806-000 Criciúma, SC, Brazil.
Fabiana C B
FC
Bernardi
Francine
F
Felisberto
Francieli
F
Vuolo
Fabricia
F
Petronilho
Daniela R
DR
Souza
Thais F
TF
Luciano
Cláudio T
CT
de Souza
Cristiane
C
Ritter
Felipe
F
Dal-Pizzol
D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000150:complications*; D015850:Interleukin-6 / Q000097:blood; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D010920:Placenta / Q000201:enzymology; D011225:Pre-Eclampsia / Q000097:blood / Q000473:pathology*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism; D050050:Protein Carbonylation; D015398:Signal Transduction; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
D015415:Biomarkers; C508600:IL6 protein, human; D015850:Interleukin-6; C507779:MYD88 protein, human; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D011257:Pregnancy Proteins; C495345:TLR4 protein, human; D017392:Thiobarbituric Acid Reactive Substances; D051197:Toll-Like Receptor 4; D009195:Peroxidase