[Simultaneous determination of 6 active components in Chrysanthemum morifolium by HPLC].
OBJECTIVE
To develop a HPLC method quantitative method for simultaneous determination of chlorogenic acid, 1, 5-dicaffeoylquinic acid, isochlorogenic acid A, isochlorogenic acid C, luteolin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside in Chrysanthemum morifolium Ramat.


METHODS
A Phenomenex Gemini-NX C18 column (4.6 mm x 250 mm, 5 microm) was used with CH3 OH and 0.4% H3PO4 as mobile phases. The flow rate was 1 mL x min(-1), the column temperature was 25 degrees C, and the detection wavelength was set at 350 nm.


RESULTS
The 6 active components were in baseline separation. The linearity of this method was good (r > or = 0.999 7), and the average recoveries were 100.6% - 102.4%, RSD < 3%. Except isochlorogenic acid A, the contents of the determined components in the steam-blanched flower heads were significantly higher than those non blanched. The contents of chlorogenic acid and isochlorogenic acid A in the steam-blanched semiopened flower heads were higher than fully opened ones by 53% and 41%, respectively.


CONCLUSIONS
The method is sensitive, accurate, reliable and repeatable, which can be used for quality evaluation of Chrysanthemum.
Institute of Pharmacognosy, School of Pharmaceutical Science, Shandong University, Jinan 250012, China. qin841124@163.com
Shan
S
Qin
Xuesen
X
Wen
D047310:Apigenin / Q000032:analysis; D002726:Chlorogenic Acid / Q000031:analogs & derivatives / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031202:Chrysanthemum / Q000737:chemistry*; D002934:Cinnamates / Q000032:analysis; D035264:Flowers / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis; D047311:Luteolin / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002934:Cinnamates; D005960:Glucosides; D010936:Plant Extracts; C469310:isochlorogenic acid; D002726:Chlorogenic Acid; C057792:apigetrin; D047310:Apigenin; C100257:cynarine; C066408:luteolin-7-glucoside; D047311:Luteolin