Novel high butanol production from lactic acid and pentose by Clostridium saccharoperbutylacetonicum.
We previously reported a butanol production process with pH-stat continuous feeding of dl-lactic acid and glucose as the co-substrate (Oshiro et al., Appl. Microbiol. Biotechnol., 87, 1177-1185, 2010). To accomplish butanol production from completely inedible substrates, in this study, we investigated acetone-butanol-ethanol (ABE) fermentation of Clostridium saccharoperbutylacetonicum N1-4 with lactic acid by using pentose as the co-substrate. Examination for optimum co-substrate indicated that arabinose was superior to glucose and xylose for ABE fermentation. Actually batch culture with lactic acid and arabinose without pH control exhibited higher butanol production (7.11 g/l) and lactic acid consumption (2.02 g) than those (6.62 g/l and 1.45 g, respectively) with glucose. Fed-batch culture without pH control increased these values to 12.08 g/l and 15.60 g/l butanol production, and to 3.83 g and 5.91 g lactic acid consumption by feeding the substrate once and twice, respectively. Finally, the result of gas chromatography-mass spectroscopy analysis using [1,2,3-(13)C(3)]-lactic acid indicated that lactic acid was converted to butanol with the efficiency of 51.9%. Thus, we established a novel high butanol production from lactic acid using arabinose as the co-substrate in simple fed-batch culture.
Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. jubeat.knit.tsuyoshi@gmail.com
Tsuyoshi
T
Yoshida
Yukihiro
Y
Tashiro
Kenji
K
Sonomoto
D000096:Acetone / Q000378:metabolism; D001089:Arabinose / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D000440:Butanols / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
D000440:Butanols; D000096:Acetone; D019344:Lactic Acid; D000431:Ethanol; D014994:Xylose; D001089:Arabinose; D005947:Glucose