A recombinant ω-gliadin-like D-type glutenin and an α-gliadin from wheat (Triticum aestivum): two immunoglobulin E binding proteins, useful for the diagnosis of wheat-dependent allergies.
Among the wheat prolamins, D-type glutenins display a highly repetitive sequence similar to ω-gliadins, but they contain a cysteine, that allows them to be included in the gluten macropolymers. An ω-gliadin-like D-type glutenin, an α-gliadin, and an ω5-gliadin-like D-type glutenin were obtained as recombinant proteins and compared using synchrotron radiation circular dichroism. This technique evidenced the strong thermostability of the ω5-gliadin-like protein. The IgE reactivity of recombinant proteins was evaluated using 45 sera from wheat-allergic patients. The sera from patients diagnosed with cutaneous hypersensitivity to hydrolyzed wheat proteins often reacted with the ω-gliadin-like D-type glutenin and α-gliadin, whereas the IgE reaction was less frequent after dietary sensitization. So, these two proteins could be useful to diagnose these diseases. The sera from patients with exercise-induced anaphylaxis recognized the ω5-gliadin-like protein as a positive control and, less frequently, the other proteins tested. Only some sera from patients with baker's asthma reacted with the proteins tested.
INRA, UMR1318, Institut Jean-Pierre Bourgin, Versailles, France.
Hamza
H
Mameri
Isabelle
I
Bouchez
Catherine
C
Pecquet
Nadia
N
Raison-Peyron
Dominique
D
Choudat
Habib
H
Chabane
Stephane
S
Kerre
Sandra
S
Denery-Papini
Yann
Y
Gohon
Pierre
P
Briozzo
Michel
M
Laurière
Jacques
J
Snégaroff
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D005903:Gliadin / Q000737:chemistry* / Q000276:immunology; D005983:Glutens / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000378:metabolism*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins; D014908:Triticum / Q000737:chemistry*; D021182:Wheat Hypersensitivity / Q000175:diagnosis* / Q000276:immunology
D002352:Carrier Proteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D005983:Glutens; D005903:Gliadin; C037302:glutenin