[Study on Th1/Th2 transcriptional regulatory mechanism of san'ao tang in bronchial asthmatic rats].
OBJECTIVE
To study the regulatory effect of San'ao Tang on Thl/Th2 cells and its interference on transcription factor GATA-3 and T-bet-mRNA in asthmatic rats.


METHODS
Rat asthma model was established by abdominal injection and aerosol inhalation of OVA. The content of cytokines IL-4 and IFN-gamma in alveolus lavage fluid of asthmatic rats was detected before and after the oral administration with large, medium, low dose of San'ao Tang and dexamethasone. T-bet and GATA-3 mRNA expressions in lung tissues were determined by RT-PCR.


RESULTS
Compared with other groups, the medium-dose group showed a notable decrease in IL-4 in rat BALF and an increasing IFN-gamma. Its IL-4/IFN-gamma is significant lower than the asthma group, with expressions of lung tissue GATA-3 and transcription factor T-bet-mRNA in line with changes in IL-4 and IFN-gamma.


CONCLUSIONS
San'ao Tang may have a inhibitory effect on airway inflammation of asthmatic rats by regulating Th1/Th2 transfer factors.
Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China. hippe007@126.com
Hui
H
Chen
Rong
R
Ma
D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018417:Th1 Cells / Q000187:drug effects / Q000378:metabolism*; D018418:Th2 Cells / Q000187:drug effects / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; C411354:san-ao-tang