Cytotoxicity and glycan-binding properties of an 18 kDa lectin isolated from the marine sponge Halichondria okadai.
A divalent cation-independent lectin-HOL-18, with cytotoxic activity against leukemia cells, was purified from a demosponge, Halichondria okadai. HOL-18 is a 72 kDa tetrameric lectin that consists of four non-covalently bonded 18 kDa subunits. Hemagglutination activity of the lectin was strongly inhibited by chitotriose (GlcNAcβ1-4GlcNAcβ1-4GlcNAc), fetuin and mucins from porcine stomach and bovine submaxillary gland. Lectin activity was stable at pH 4-12 and temperatures lower than 60 °C. Frontal affinity chromatography with 16 types of pyridylaminated oligosaccharides indicated that the lectin had an affinity for N-linked complex-type and sphingolipid-type oligosaccharides with N-acetylated hexosamines and neuramic acid at the non-reducing termini. The lectin killed Jurkat leukemia T cells and K562 erythroleukemia cells in a dose- and carbohydrate-dependent manner.
Laboratory of Glycobiology and Marine Biochemistry, Department of Genome System Science, Graduate School of NanoBio Sciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan. akagiwashizuten@yahoo.co.jp
Ryo
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Matsumoto
Yuki
Y
Fujii
Sarkar M A
SM
Kawsar
Robert A
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Kanaly
Hidetaro
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Yasumitsu
Yasuhiro
Y
Koide
Imtiaj
I
Hasan
Chihiro
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Iwahara
Yukiko
Y
Ogawa
Chang Hun
CH
Im
Shigeki
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Sugawara
Masahiro
M
Hosono
Kazuo
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Nitta
Jiharu
J
Hamako
Taei
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Matsui
Yasuhiro
Y
Ozeki
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000378:metabolism / Q000494:pharmacology*; D006386:Hemagglutination Tests; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D011134:Polysaccharides / Q000378:metabolism*; D011161:Porifera
D003603:Cytotoxins; D037102:Lectins; D011134:Polysaccharides