Effect of lavender essential oil on LPS-stimulated inflammation.
Lavender essential oil (LEO) is one the most favorite and widely used essential oils in aromatherapy. Many studies have demonstrated its functions in calming, assisting sleep, reducing pain and muscular spasms and its antiseptic function. To date, however, the mechanism of LEO on inflammation response is not well understood. In this study, we examined the effect of LEO on 5 μg/ml lipopolysaccharide (LPS) induced inflammation reaction in human monocyte THP-1 cells. We found treatment of 0.1% LEO significantly increased cell viability and inhibited the IL-1β and superoxide anion generation in LPS-stimulated THP-1 cells. Treatment with LEO down-regulated both LPS-induced protein levels of phospho-NF-κB and membrane Toll-like receptor 4. To determine whether the chaperone protein was involved in the reaction, we determined the levels of Heat Shock Protein 70 (HSP70). Our results showed that LEO increased HSP70 expression in LPS-stimulated THP-1 cells, suggesting that the LEO inhibited LPS-induced inflammatory effect might be associated with the expression of HSP70.
Department of Nursing, Yuanpei University, Hsinchu, Taiwan.
Mei-Yu
MY
Huang
May-Hua
MH
Liao
Yang-Kao
YK
Wang
Yung-Sheng
YS
Huang
Hsiao-Chuan
HC
Wen
D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D027523:Lavandula; D008070:Lipopolysaccharides / Q000633:toxicity*; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D009822:Oils, Volatile / Q000494:pharmacology / Q000627:therapeutic use*; D010938:Plant Oils / Q000494:pharmacology / Q000627:therapeutic use*; D051197:Toll-Like Receptor 4 / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009822:Oils, Volatile; D010938:Plant Oils; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; C045718:lavender oil