Exosome can prevent RNase from degrading microRNA in feces.
BACKGROUND
Because the stability of miRNA in feces has not been clarified, we examined the stability of miRNA in feces.


METHODS
RNase was added into culture media of HT-29 cells and fecal homogenates. The relative quantifications of miRNA were analyzed by real-time RT-PCR.


RESULTS
Cellular miRNA or exosomal miRNA were protected from RNase by the cellular membrane or the exosome; meanwhile, free miRNA was degraded immediately and completely by RNase.


CONCLUSIONS
The present study revealed that exosome or cellular membrane could prevent RNase from degrading miRNA inside the exosome or cells even in a dreadful condition, as in feces.
Investigative Treatment Division, Research Center for Innovative Oncology, National Cancer Center Hospital East, Kashiwa;
Yoshikatsu
Y
Koga
Masahiro
M
Yasunaga
Yoshihiro
Y
Moriya
Takayuki
T
Akasu
Shin
S
Fujita
Seiichiro
S
Yamamoto
Yasuhiro
Y
Matsumura