[The effect of oxidative stress on aorta endothelial cell in chronic intermittent hypoxia animal model].
OBJECTIVE
To make a chronic intermittent hypoxia (CIH) animal model and explore the relationships of oxidative stress and the injury of aorta endothelial cell on the CIH rat model.


METHODS
Twenty-four sex maturation rat were randomly Divided into unhandled control group(normal feeding for 4 weeks, UC group), CIH group (CIH for 4 weeks), reoxygenation group (normal feeding for 4 weeks after CIH for 4 weeks, RH group). Determined the activity of superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) in blood serum of all groups. Dislodged rat's aorta, observed the appearance of aorta endothelial cell and the expression of endothelial nitric oxide synthase (eNOS).


RESULTS
Compared with UC group, in CIH group the activities of SOD and NO were significantly lower, but MDA significantly higher (all of P < 0.05 respectively), the appearance of aorta endothelial cell changed, the expressions of eNOS lower. There were not significantly change in UC group and RH group (P > 0.05).


CONCLUSIONS
Oxidative stress played a role in the injury of aorta endothelial cell on ob chronic intermittent hypoxia rat modle.
Department of Otorhinolaryngology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Jie
J
Li
Bing
B
Li
Xiang
X
Yang
Hailan
H
Mo
D000818:Animals; D001011:Aorta / Q000378:metabolism; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism*; D000860:Hypoxia / Q000097:blood*; D008315:Malondialdehyde / Q000097:blood*; D009569:Nitric Oxide / Q000097:blood; D052250:Nitric Oxide Synthase Type III / Q000097:blood*; D018384:Oxidative Stress / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D013482:Superoxide Dismutase / Q000097:blood*
D009569:Nitric Oxide; D008315:Malondialdehyde; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D013482:Superoxide Dismutase