Cloning, sequence, and association analysis of porcine OSAP gene.
Ovary-specific acidic protein (OSAP) is an important reproduction-related gene. In this study, we cloned the full-length cDNA sequence of porcine OSAP gene through the rapid amplification of cDNA ends method. The porcine OSAP gene encodes a protein of 238 amino acids which shares high homology with the OSAP of five species: rhesus monkey (72 %), human (70 %), cattle (68 %), horse (68 %), and dog (61 %). This gene is structured in four exons and three introns as revealed by computer-assisted analysis. PCR-RFLP was established to detect the GU373669:c.854A>T substitution of porcine OSAP gene mRNA and association of this mutation with litter size traits was assessed in Large White (n=100) and Landrace (n=100) pig populations. Results demonstrated that this polymorphic locus was significantly associated with the litter size of all parities in Large White sows and Landrace sows (P<0.05). Therefore, OSAP gene could be a useful candidate gene in selection for increasing litter size in pigs. These data serve as a foundation for further insight into this novel porcine gene.
College of Agronomy/Urban Modern Agriculture Engineering Research Center, Kunming University, 650214 Kunming, China.
Yu
Y
Lei
Liu
L
Yonggang
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D006801:Humans; D008098:Litter Size / Q000235:genetics; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D012098:Reproduction / Q000235:genetics; D017421:Sequence Analysis; D013552:Swine / Q000235:genetics* / Q000502:physiology
D018076:DNA, Complementary; D011506:Proteins