Expression of heparanase-1 in prostate gland tumors.
Expression of heparanase-1 in prostate tumors was evaluated by RT-PCR, immunoblotting, and immunohistochemistry. Malignant transformation was shown to be associated with considerable increase in the expression of heparanase-1 at both mRNA and protein levels, which correlated with the degree of metastasizing and can be used as the marker for diagnostics of the metastatic process.
Institute of Molecular Biology and Biophysics, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia. olesyakutsenko@mail.ru
O S
OS
Kutsenko
A V
AV
Kovner
L A
LA
Mostovich
I S
IS
Kunin
R D
RD
Nepomnyashchikh
T Yu
TY
Prudnikova
E V
EV
Grigor'eva
D017931:DNA Primers / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000175:diagnosis*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D000099175:Heparanase
D017931:DNA Primers; D005966:Glucuronidase; D000099175:Heparanase