[Influences of nevirapine on cell proliferation and expression of sodium/iodide symporter in human anaplastic thyroid carcinoma cell line].
OBJECTIVE
To explore the effects of nevirapine on the proliferation and expression of sodium-iodide symporter (NIS) mRNA in FRO cells in vitro.


METHODS
The cells were incubated in different concentrations of nevirapine to evaluate the cell growth rate. And the expressions of NIS mRNA and TSHR mRNA were determined by real-time quantitative reverse polymerase chain reaction.


RESULTS
Cell proliferation was inhibited after a 96 h nevirapine treatment. After incubating in the presence of 200 and 350 µmol/L nevirapine, the expression of NIS mRNA was (1.39 ± 0.04) and (1.85 ± 0.28) times versus the control cells (P < 0.01) while the expressions of TSHR mRNA was (2.23 ± 1.47) and (2.83 ± 0.78) times versus the control cells respectively (both P < 0.05).


CONCLUSIONS
Nevirapine inhibits cell proliferation in FRO cell line. More importantly, the cells exposed to nevirapine may induce the up-regulations of NIS mRNA and TSHR mRNA.
Department of Endocrinology, Qianfoshan Hospital, Shandong University, Jinan 250014, China.
Ying
Y
Zhou
Jian-jun
JJ
Dong
Huan-jun
HJ
Wang
Zhong-wen
ZW
Zhang
Lin
L
Liao
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D019829:Nevirapine / Q000494:pharmacology*; D011989:Receptors, Thyrotropin / Q000378:metabolism; D027981:Symporters / Q000378:metabolism*; D065646:Thyroid Carcinoma, Anaplastic; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology*; D000099209:Sodium-Iodide Symporters
D019829:Nevirapine; D011989:Receptors, Thyrotropin; D027981:Symporters; D000099209:Sodium-Iodide Symporters