[Simultaneous determination of five active components in resina draconis and its extract by HPLC].
OBJECTIVE
To establish an HPLC method for simultaneous determination of contents of loureirin A, loureirin B, 7,4'-dihydroxy flavone, pterostilbene and resveratrol in resina draconis and its extracts.


METHODS
Kromasil 100-5C18 column (4.6 mm x 250 mm, 5 microm) was used with the mobile phase of acetonitrile-1% glacial acetic acid at a flow rate of 1.0 mL x min(-1) and the column temperature at 40 degrees C. The detective wave length of loureirin A and B was detected at 278 nm, and 7,4'-dihydroxy flavone, pterostilbene and resveratrol was at 319 nm.


RESULTS
All the five active components reached the resolved peaks within 40 min, indicating a good linearity (r > or = 0.999 7). The average recoveries of loureirin A, loureirin B, 7,4'-dihydroxy flavone, pterostilbene and resveratrol in resina draconis were 102.9%, 96.81%, 97.29%, 100.7% and 103.7%, with RSDs of 0.23%, 1.5%, 0.42%, 0.58% and 0.34%, respectively. The average recoveries of loureirin A, loureirin B, 7,4'-dihydroxy flavone, pterostilbene and resveratrol in extract of resina draconis were 102. 2% , 96. 93%, 97. 90% , 102.0% and 103.3%, with RSDs of 1.7%, 0.91%, 1.4%, 1.5% and 1.2%, respectively.


CONCLUSIONS
The method is so easy, accurate, highly repeatable and stable that it provides good reference for the quality control of resina draconis and its extracts.
Nanjing University of Chinese Medicine, Jiangsu 210000, China. liyun3648@163.com
Yun
Y
Li
Wei
W
Xiao
Jianping
J
Qin
Ying
Y
Guo
Qianqian
Q
Zhu
D047188:Chalcones / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031409:Dracaena / Q000737:chemistry*; D047309:Flavones / Q000032:analysis; D012116:Resins, Plant / Q000032:analysis; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis
D047188:Chalcones; D047309:Flavones; D012116:Resins, Plant; D013267:Stilbenes; C560420:loureirin A; C107773:pterostilbene; C505764:loureirin B; D000077185:Resveratrol; C043562:flavone