Preliminary safety assessment of a membrane-bound delta 9 desaturase candidate protein for transgenic oilseed crops.
A gene encoding delta 9 desaturase (D9DS), an integral membrane protein, is being considered for incorporation into oilseed crops to reduce saturated fatty acids and thus improve human nutritional value. Typically, a safety assessment for transgenic crops involves purifying heterologously produced transgenic proteins in an active form for use in safety studies. Membrane-bound proteins have been very difficult to isolate in an active form due to their inherent physicochemical properties. Described here are methods used to derive enriched preparations of the active D9DS protein for use in early stage safety studies. Results of these studies, in combination with bioinformatic results and knowledge of the mode of action of the protein, along with a history of safe consumption of related proteins, provides a weight of evidence supporting the safety of the D9DS protein in food and feed.
Dow AgroSciences LLC, 9330 Zionsville Road, IN 46268, USA. kmmadduri@dow.com
Krishna M
KM
Madduri
Barry W
BW
Schafer
James M
JM
Hasler
Gaofeng
G
Lin
Mendy L
ML
Foster
Shawna K
SK
Embrey
Lakshmi
L
Sastry-Dent
Ping
P
Song
Ignacio M
IM
Larrinua
Daniel J
DJ
Gachotte
Rod A
RA
Herman
D016367:Baculoviridae; D002462:Cell Membrane; D018556:Crops, Agricultural / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D009753:Nutritive Value; D010938:Plant Oils / Q000737:chemistry*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000737:chemistry*; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism*
D005227:Fatty Acids; D010938:Plant Oils; D013230:Stearoyl-CoA Desaturase