Effect of ladasten on the content of cytokine markers of inflammation and behavior of mice with experimental depression-like syndrome.
The effects of ladasten and reference product imipramine (10 mg/kg) on the content of cytokines TNF-α, IL-6, IL-10, IL-1α, IL-2, IL-4, IL-5, IL-17, IFN-γ, and GM-CSF and behavior of male C57Bl/6 mice were studied on the model of a depression-like state induced by a single intraperitoneal injection of bacterial LPS in a dose of 100 μg/kg. Ladasten was injected 5 times in doses of 30 and 50 mg/kg. LPS was administered 1 h after the last injection of the test agents. Behavioral disturbances and significant increase in the concentration of TNF-α and IL-6 in blood plasma were observed 2 h after LPS treatment. Ladasten was more potent than imipramine in decreasing the content of proinflammatory cytokines TNF-α and IL-6 and preventing the development of behavioral disturbances in mice. Antiasthenic drug ladasten was shown to decrease the elevated level of proinflammatory cytokines TNF-α and IL-6 after LPS treatment. Further studies should be performed to develop new indications for the use of ladasten in adjuvant therapy of depression.
V. V. Zakusov Institute of Pharmacology, Russian Academy of Medical Sciences, Moscow. Russia. annatall@rambler.ru
A V
AV
Tallerova
L P
LP
Kovalenko
A D
AD
Durnev
S B
SB
Seredenin
D000218:Adamantane / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016207:Cytokines / Q000097:blood*; D003863:Depression / Q000188:drug therapy* / Q000276:immunology; D007099:Imipramine / Q000494:pharmacology / Q000627:therapeutic use; D018836:Inflammation Mediators / Q000097:blood*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000187:drug effects
D000928:Antidepressive Agents; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; C493151:N-(2-adamantyl)-N-p-bromophenylamine; D007099:Imipramine; D000218:Adamantane