Protocol for the use of light upon extension real-time PCR for the determination of viral load in HBV infection.
Real-time PCR has engendered wide acceptance for quantitation of hepatitis B virus (HBV) DNA in the blood due to its improved rapidity, sensitivity, reproducibility, and reduced contamination. Here we describe a cost-effective and highly sensitive HBV real-time quantitative assay based on the light upon extension real-time PCR platform and a simple and reliable HBV DNA preparation method using silica-coated magnetic beads.
School of Life Sciences, Northeast Normal University, Changchun, People's Republic of China.
Guimin
G
Li
Wangfeng
W
Li
Lixia
L
Liu
D003362:Cost-Benefit Analysis; D017931:DNA Primers / Q000235:genetics*; D004279:DNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D006509:Hepatitis B / Q000097:blood / Q000821:virology*; D006515:Hepatitis B virus / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D057230:Limit of Detection; D059346:Magnets; D008863:Microspheres; D025202:Molecular Diagnostic Techniques; D060888:Real-Time Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry; D019562:Viral Load / Q000379:methods*
D017931:DNA Primers; D004279:DNA, Viral; D012822:Silicon Dioxide