[The influence of macromolecular excipients on the process of mass transfer at the boundary phase from a model anti-dandruff preparation into the external compartment under in vitro conditions].
OBJECTIVE
The aim of the research was the application assessment of a model preparation--anti-dandruff emulsion. The experiments was conducted under in vitro conditions using of a model system of human skin, which are cellulose dialysis membranes with a standardized pore size. The behaviour of the preparation on the dialysis membrane was analyzed, and the degree of film formation performing the function of a protective barrier was evaluated after gentle rinsing with tepid water without cleaning agents. It was checked whether the product layer remaining on the membrane product layer does not block diffusion and penetration processes. Additionally, an attempt to assess the character of the layer with the use of a hydrophilic marker was made.


METHODS
Model anti-dandruff emulsion, Servapor Dialysis Tubing 100 dialysis membranes with a wall thickness d = 0.1 mm and a declared pore diameter phi = 25 A degrees, sodium chloride, purified water--assessment of the residue level of the analyzed preparation on the dialysis membrane. Examination on the marker diffusion through the dialysis membrane covered with the model anti-dandruff preparation, Approximation of the obtained results.


RESULTS
The first phase of the experiment was the preparation of dialysis membranes for the adsorption process of a model anti-dandruff preparation, then its adsorption, rinsing with tepid water and the measurement of residue level of the product on the membrane control. It has been observed that the mass of the membrane on which the preparation was applied increased in relation to the membrane from a control sample. The mass increase of the membrane in a research sample is accompanied with the increase of its thickness caused by the adsorbed amount of the product and water on the membrane. The diffusion through the membrane in a control sample turned out to be slightly slower what may indicate the character of the membrane residue of the model anti-dandruff preparation. CONCLUSIONS; The technology of model anti-dandruff emulsion enables the formation of a layer forming a specific protective barrier on the surface of the dialysis membrane. The presence of the layer of model anti-dandruff emulsion on the membrane is confirmed by mean values of mass and thickness increase of the dialysis membrane. The hydrophobic diffusion layer is of a variable character. The properly applied emulsion under "in vivo" conditions might fill voids of the corneal layer of epidermis creating a structure of a film character performing the function of a protective barrier. On the basis of the in vitro assessment, the therapeutic effectiveness under in vivo conditions may be concluded.
Zakład Technologii Postaci Leku, Katedra Farmacji Stosowanej, Uniwersytet Medyczny w Łodzi, ul. Muszyńskiego 1, 90-151 Łódź. michal.kolodziejczyk@umed.lodz.pl
Michał Krzysztof
MK
Kołodziejczyk
Marian Mikołaj
MM
Zgoda
D000327:Adsorption; D002626:Chemistry, Pharmaceutical; D012628:Dermatitis, Seborrheic / Q000517:prevention & control; D003879:Dermatologic Agents / Q000737:chemistry*; D004058:Diffusion; D004655:Emulsions; D005079:Excipients / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D008954:Models, Biological; D012867:Skin / Q000378:metabolism*; D012915:Soaps / Q000737:chemistry*
D003879:Dermatologic Agents; D004655:Emulsions; D005079:Excipients; D008567:Membranes, Artificial; D012915:Soaps