Construction of a stable genetically engineered rhamnolipid-producing microorganism for remediation of pyrene-contaminated soil.
One rhamnolipid-producing bacterial strain named Pseudomonas aeruginosa BSFD5 was isolated and characterized. Its rhlABRI cassette including necessary genes for rhamnolipid synthesis was cloned and transformed into the chromosome of P. putida KT2440 by a new random transposon vector without introducing antibiotic-resistance marker, generating a genetically engineered microorganism named P. putida KT2440-rhlABRI, which could stably express the rhlABRI cassette and produce rhamnolipid at a yield of 1.68 g l(-1). In experiments using natural soil, it was shown that P. putida KT2440-rhlABRI could increase the dissolution of pyrene and thus promote its degradation by indigenous microorganisms. P. putida KT2440-rhlABRI thus demonstrated potential for enhancing the remediation of soils contaminated with polycyclic aromatic hydrocarbons.
Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Li
L
Cao
Qian
Q
Wang
Ji
J
Zhang
Chao
C
Li
Xin
X
Yan
Xu
X
Lou
Yali
Y
Xia
Qing
Q
Hong
Shunpeng
S
Li
D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D030541:Databases, Genetic; D005798:Genes, Bacterial; D006017:Glycolipids / Q000096:biosynthesis*; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism; D011721:Pyrenes / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D006017:Glycolipids; D011721:Pyrenes; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C418382:rhamnolipid; C030984:pyrene