Ganoderma tsugae Induces S Phase Arrest and Apoptosis in Doxorubicin-Resistant Lung Adenocarcinoma H23/0.3 Cells via Modulation of the PI3K/Akt Signaling Pathway.
Ganoderma tsugae (GT) is a traditional Chinese medicine that exhibits significant antitumor activities against many types of cancer. This study investigated the molecular mechanism by which GT suppresses the growth of doxorubicin-resistant lung adenocarcinoma H23/0.3 cells. Our results reveal that GT inhibits the viability of H23/0.3 cells in vitro and in vivo and sensitizes the growth suppression effect of doxorubicin on H23/0.3 cells. The data also show that GT induces S phase arrest by interfering with the protein expression of cyclin A, cyclin E, CDK2, and CDC25A. Furthermore, GT induces cellular apoptosis via induction of a mitochondria/caspase pathway. In addition, we also demonstrate that the suppression of cell proliferation by GT is through down-regulation of the PI3K/Akt signaling pathway. In conclusion, this study suggests that GT may be a useful adjuvant therapeutic agent in the treatment of lung cancer.
Graduate Institute of Clinical and Medical Science, School of Medicine, China Medical University, Taichung 40402, Taiwan.
Yang-Hao
YH
Yu
Han-Peng
HP
Kuo
Hui-Hsia
HH
Hsieh
Jhy-Wei
JW
Li
Wu-Huei
WH
Hsu
Shih-Jung
SJ
Chen
Muh-Hwan
MH
Su
Shwu-Huey
SH
Liu
Yung-Chi
YC
Cheng
Chih-Yi
CY
Chen
Ming-Ching
MC
Kao