[Screening key genes associated with the development and progression of non-small cell lung cancer based on gene-enrichment analysis and meta-analysis].
BACKGROUND
Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors; however, its causes are still not completely understood. This study was designed to screen the key genes and pathways related to NSCLC occurrence and development and to establish the scientific foundation for the genetic mechanisms and targeted therapy of NSCLC.


METHODS
Both gene set-enrichment analysis (GSEA) and meta-analysis (meta) were used to screen the critical pathways and genes that might be corretacted with the development and progression of lung cancer at the transcription level.


RESULTS
Using the GSEA and meta methods, focal adhesion and regulation of actin cytoskeleton were determined to be the more prominent overlapping significant pathways. In the focal adhesion pathway, 31 genes were statistically significant (P<0.05), whereas in the regulation of actin cytoskeleton pathway, 32 genes were statistically significant (P<0.05).


CONCLUSIONS
The focal adhesion and the regulation of actin cytoskeleton pathways might play important roles in the occurrence and development of NSCLC. Further studies are needed to determine the biological function for the positive genes.
Department of Cardiothoracic Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning, China.
Wenwu
W
He
Lei
L
Xian
Yongyong
Y
Wang
Yanling
Y
Hu
Mingwu
M
Chen
D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology*; D019295:Computational Biology / Q000379:methods*; D018450:Disease Progression; D052138:Genes, Neoplasm / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*