Analysis of genetic diversity among Chinese Pleurotus citrinopileatus Singer cultivars using two molecular marker systems (ISSRs and SRAPs) and morphological traits.
To evaluate the genetic diversity of Pleurotus citrinopileatus Singer cultivars in China, 20 P. citrinopileatus strains were analyzed using morphological traits, inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) molecular markers. Eleven ISSR primers amplified a total of 116 DNA fragments of which 96 (82.91%) were polymorphic, whereas 8 SRAP primer pairs amplified 69 fragments of which 65 (93.47%) were polymorphic. Phylogenetic trees constructed on the basis of ISSR, SRAP, and combined ISSR/SRAP analyses using the Unweighted Pair-group Method with Arithmetic Averages method distributed the 20 strains into three or six major groups. The grouping exhibited great similarity and was generally consistent with their morphological characters and antagonism test, which indicated a high level of genetic diversity among P. citrinopileatus Singer and relationship between each other. Based on the genetic analysis, the primary mini-core strains were constructed with progressive sampling method of the smallest genetic distance. The mini-core germplasm collection included 4 strains (strain 2, 5, 7 and 11). Our findings will provide a scientific fundament for facilitating parent selection for broadening genetic base, accelerating the genetic breeding, identification of cultivated strains and the development of bioactive products from this commercially important medicinal mushroom.
School of Life Sciences, Ludong University, Yantai 264025, China. qiushengzhang@126.com
Qiu-Sheng
QS
Zhang
Bing-Lian
BL
Xu
Lin-de
LD
Liu
Qin-Qin
QQ
Yuan
Hong-Xin
HX
Dong
Xian-Hao
XH
Cheng
De-Ling
DL
Lin
D002681:China; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D010802:Phylogeny; D020076:Pleurotus / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004271:DNA, Fungal