Molecular and catalytic properties of 2,4-diacetylphloroglucinol hydrolase (PhlG) from Pseudomonas sp. YGJ3.
Gene phlG encoding 2,4-diacetylphloroglucinol hydrolase was cloned from Pseudomonas sp. YGJ3 and expressed in Escherichia coli. Recombinant PhlG was purified homogeneously. It required 2-mercaptoethanol for stability. Km for 2,4-diacetylphloroglucinol and kcat were determined to be 24 µM and 5.8 s(-1) respectively. CoCl2 specifically and significantly activated PhlG.
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan.
Hiroki
H
Saitou
Manami
M
Watanabe
Kiyofumi
K
Maruyama
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D003035:Cobalt / Q000737:chemistry; D004789:Enzyme Activation; D004795:Enzyme Stability; D004926:Escherichia coli; D015870:Gene Expression; D006867:Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008623:Mercaptoethanol / Q000737:chemistry; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D003035:Cobalt; D008623:Mercaptoethanol; C059817:2,4-diacetylphloroglucinol; D010696:Phloroglucinol; D006867:Hydrolases; C018021:cobaltous chloride