Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex.
A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Arash
A
Ghodousi
Ghodousi A
GA
Arash
S
S
Vatani
Davood
D
Darban-Sarokhalil
Maryam
M
Omrani
A
A
Fooladi
Aa
A
Fooladi
A
A
Khosaravi
Ad
A
Khosaravi
Mohammad Mehdi
MM
Feizabadi