Analysis of IL1 gene polymorphisms and transcript levels in periodontal and chronic kidney disease.
Chronic kidney disease (CKD) and periodontitis (PD) are complex inflammatory disturbances, influenced by genetic factors. Interleukin (IL)-1 genes code for inflammatory mediators involved in the physiopathogenesis of both diseases. Functional polymorphisms in IL1 genes modulate cytokine levels and have been associated with susceptibility to immune-inflammatory conditions.


OBJECTIVE
The aim of this study was investigate the association of functional IL1 gene polymorphisms and transcript levels with susceptibility to CKD and PD.


METHODS
The sample consisted of 246 individuals, mean age 44.8 years, divided into: group 1 (64 patients without CKD and without PD), group 2 (58 without CKD and with PD), group 3 (52 with CKD and without PD) and group 4 (72 with CKD and with PD). DNA was obtained from cells of oral mucosa and polymorphisms IL1AC-889T, IL1BC-511T, IL1BC+3954T and IL1RN (intron 2) were analyzed by PCR-RFLP. Transcript levels from gingival tissues were analyzed by real-time PCR.


RESULTS
IL1RN(*)1 allele was associated with almost 4-fold increased risk for CKD (OR 3.92 95% CI=1.6-9.4, p=0.002). IL1RN(*)2 allele was associated with 3-fold increased risk for PD in CKD patients (OR 3.08 95% CI=1.2-7.9, p=0.019). Allele T for polymorphism IL1B+3954 was associated with CKD in PD patients (OR 2.28 95% CI=1.1-4.7, p=0.019). Significantly increased levels of transcripts of IL1A, IL1B and IL1RN genes were found in PD patients.


CONCLUSIONS
It was observed an evidence for association of IL1B and IL1RN alleles with susceptibility to CKD and PD. Higher levels of IL1 gene transcripts were found in PD patients.
Health and Biosciences School, Pontifícia Universidade Católica do Paraná (PUCPR), Imaculada Conceição Street 1155, CEP 80215-901, Curitiba, PR, Brazil.
Ana Paula Ribeiro
AP
Braosi
Cleber Machado
CM
de Souza
Sônia Mara
SM
Luczyszyn
Acir José
AJ
Dirschnabel
Marcela
M
Claudino
Márcia
M
Olandoski
Christian Macagnan
CM
Probst
Gustavo Pompermaier
GP
Garlet
Roberto
R
Pecoits-Filho
Paula Cristina
PC
Trevilatto
D000328:Adult; D000368:Aged; D000483:Alleles; D000704:Analysis of Variance; D016009:Chi-Square Distribution; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics; D007375:Interleukin-1 / Q000235:genetics*; D053582:Interleukin-1alpha / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000235:genetics*; D011110:Polymorphism, Genetic; D051436:Renal Insufficiency, Chronic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D014158:Transcription, Genetic; D055815:Young Adult
D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D053582:Interleukin-1alpha; D053583:Interleukin-1beta