Effect of histamine on contractile activity of smooth muscles in bovine mesenteric lymph nodes.
The effects of histamine and mechanisms of its action on the capsular smooth muscle cells of mesenteric lymph nodes were examined on isolated capsular strips under isometric conditions. Histamine (1×10(-8)-5×10(-7) M) decreased the tone of capsular smooth muscle cells and the frequency of phasic contractions. At high concentrations (more than 5×10(-6) M), histamine increased the amplitude and frequency of phasic contractions against the background of increased tonic stress. The effects of histamine were dose-dependent and were realized via direct stimulation of H(1)- and H(2)-receptors on the membrane of smooth muscle cells.
Laboratory of Blood Circulation Physiology, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia. gilobov@yandex.ru
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Lobov
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Pan'kova
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002927:Cimetidine / Q000494:pharmacology; D004155:Diphenhydramine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D007537:Isometric Contraction / Q000187:drug effects* / Q000502:physiology; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008643:Mesentery / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D011969:Receptors, Histamine H1 / Q000378:metabolism; D011970:Receptors, Histamine H2 / Q000378:metabolism; D046509:Tissue Culture Techniques
D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D002927:Cimetidine; D006632:Histamine; D004155:Diphenhydramine; D000109:Acetylcholine