Phytochemical and biological activity studies of the Bhutanese medicinal plant Corydalis crispa.
The chemical constituents and biological activities of Corydalis crispa (Fumariaceae) were investigated for the first time. The phytochemical study resulted in the isolation of nine known isoquinoline alkaloids: protopine (1), 13-oxoprotopine (2), 13-oxocryptopine (3), stylopine (4), coreximine (5), rheagenine (6), ochrobirine (7), sibiricine (8) and bicuculline (9), with complete NMR data for 2 and 3 provided here for the first time. Crude extracts exhibitedsignificant anti-inflammatory (p < 0.01) activity against TNF-alpha production in LPS activated THP-1 cells. The acetylcholinesterase inhibitory activity of compounds 2, 4 and 7 and the antiplasmodial activity of compound 5 against P. falciparum strains TM4/8.2 and K1CB1 (multidrug resistant strain) are reported here for the first time. Stylopine (4) did not show antimalarial activity against the K1CB1 strain in contrast to a previous report. This study generated a scientific basis for the use of this plant in Bhutanese traditional medicine, either individually or in combination with other medicinal ingredients to treat a broad range of disorders. This study also identified compound 5 as potential new antimalarial lead compound.
School of Chemistry, University of Wollongong, Wollongong, NSW, 2522, Australia.
Phurpa
P
Wangchuk
Paul A
PA
Keller
Stephen G
SG
Pyne
Thanapat
T
Sastraruji
Malai
M
Taweechotipatr
Roonglawan
R
Rattanajak
Aunchalee
A
Tonsomboon
Sumalee
S
Kamchonwongpaisan
D000470:Alkaloids / Q000032:analysis; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000962:Antimalarials / Q000494:pharmacology; D001631:Bhutan; D031311:Corydalis / Q000737:chemistry*; D007546:Isoquinolines / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D000962:Antimalarials; D007546:Isoquinolines; D010936:Plant Extracts