Transdifferentiation of human amniotic epithelial cells into acinar cells using a double-chamber system.
This study investigated the transdifferentiation of stem cells from human amnion tissue into functional acinar cells (ACs) using a co-culture system. Human amniotic epithelial cells (hAECs) were isolated from amnion tissue by mechanical mincing and enzymatic digestion. After primary culture, the phenotype of the cells was identified by flow cytometry (FCM) and immunocytochemical staining. hAECs were co-cultured with submandibular gland acinar cells of SD rats using a double-chamber system. The expression of α-amylase was determined by immunocytochemical method and fluorescent real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) after induction for 1 and 2 weeks, respectively. Digestion with trypsin is an effective method for isolating hAECs from amnion tissue. These cells were positive for CD29 and CK19 and weakly positive for CD44 and α-amylase. Within 2 weeks, α-amylase in hAECs increased with induction time. The expression of α-amylase in hAECs was increased 3.38-fold after co-culturing for 1 week. This ratio increased to 6.6-fold, and these cells were positive for mucins, after co-culturing for 2 weeks. hAECs possess the potential to differentiate into ACs in vitro. They might be a stem cell resource for clinical applications of cell replacement therapy in salivary gland dysfunction diseases.
Stomatological Hospital, ZunYi Medical College, ZunYi, China. guilinhuang@yeah.net
Gui-Lin
GL
Huang
Ni-Ni
NN
Zhang
Jun-Sheng
JS
Wang
Li
L
Yao
Yu-Jie
YJ
Zhao
Yu-Ying
YY
Wang
D061354:Acinar Cells / Q000166:cytology* / Q000378:metabolism; D000650:Amnion / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D054338:Cell Transdifferentiation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002478:Cells, Cultured; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D006801:Humans; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012466:Salivary Gland Diseases / Q000628:therapy; D013363:Submandibular Gland / Q000166:cytology* / Q000378:metabolism