Raman microspectroscopy for species identification and mapping within bacterial biofilms.
: A new method of mapping multiple species of oral bacteria in intact biofilms has been developed, using the optical technique of confocal Raman microscopy. A species classification algorithm, developed on dried biofilms, was used to analyze spectra of hydrated biofilms containing two microbial species central to dental health: Streptococcus sanguinis and Streptococcus mutans. The algorithm transferred successfully to the hydrated environment, correctly identifying the species of origin of single-species biofilms. We then used the algorithm successfully both to detect the presence of two species in mixed biofilms and to create spatial maps within these biofilms.
The Institute of Optics, University of Rochester, 275 Hutchison Rd,, Rochester, NY 14627, USA. ajberger@optics.rochester.edu.
Brooke D
BD
Beier
Robert G
RG
Quivey
Andrew J
AJ
Berger