HER2 monoclonal antibody conjugated RNase-A-associated CdTe quantum dots for targeted imaging and therapy of gastric cancer.
Successful development of safe and effective nanoprobes for targeted imaging and selective therapy of in-situ gastric cancer is a great challenge. Herein, one kind of multifunctional HER2 monoclonal antibody conjugated RNase A-associated CdTe quantum dot cluster (HER2-RQDs) nanoprobes was prepared, its cytotoxicity was evaluated. Subcutaneous gastric cancer nude mouse models and in-situ gastric cancer SCID mouse models were established, and were intravenously injected HER2-RQDs nanoprobes, the bio-distribution and therapeutic effects of HER2-RQDs in vivo were evaluated. Results showed that HER2-RQDs nanoprobes could selectively kill gastric cancer MGC803 cells, could target imaging subcutaneous gastric cancer cells at 3 h post-injection, and in-situ gastric cancer cells at 6 h post-injection, and could inhibit the growth of gastric cancer tissues and extended survival time of gastric cancer bearing mouse models, which is closely associated with destroying functional RNAs in cytoplasm by RNase A released from HER2-RQDs nanoprobes, preventing protein synthesis and inducing cell apoptosis. High-performance HER2-RQDs nanoprobes exhibit great potential in applications such as in-situ gastric cancer targeted imaging, and selective therapy in the near future.
Department of Bio-Nano Science and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of Ministry of Education, Shanghai Jiao Tong University, Shanghai, PR China.
Jing
J
Ruan
Hua
H
Song
Qirong
Q
Qian
Chao
C
Li
Kan
K
Wang
Chenchen
C
Bao
Daxiang
D
Cui
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D019187:Cadmium Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D003952:Diagnostic Imaging / Q000379:methods; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D036103:Nanotechnology / Q000379:methods; D009368:Neoplasm Transplantation; D045663:Quantum Dots; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry* / Q000276:immunology; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000276:immunology* / Q000628:therapy*; D013691:Tellurium / Q000737:chemistry*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D019187:Cadmium Compounds; D018719:Erb-b2 Receptor Tyrosine Kinases; D012259:Ribonuclease, Pancreatic; D013691:Tellurium; C028337:cadmium telluride