Molecular detection of the Aeromonas virulence aerolysin gene in retail meats from different animal sources in Egypt.
Meat commonly contain the same Aeromonas spp. which occur in human diarrhoeal and non-diarrhoeal faecal samples. Motile Aeromonas were isolated from 5.6% of total 302 samples. The distribution of the isolates were 5.9 and 5.2% in fresh and frozen samples, respectively. Of the 302 samples taken of the four animal meat species investigated, the genus Aeromonas were isolated in 12.3% of the fresh samples collected from buffalo meat, in 6.5% of the samples collected from sheep meat and 14.0% from the samples collected from the cattle frozen meat samples. The camel meat did not reveal any Aeromonas isolates. Aeromonas hydrophila was isolated as the most prevalent species with 6.8%, followed by Aeromonas caviae with 2.7% and Aeromonas sobria with 2.1% from the total meat samples. Aerolysin toxin gene (aerA) was detected in 3/17 isolates of A. hydrophila isolated from contaminated meat. Infection due to bacterial pathogen with such virulent factor through contact with contaminated meat while handling them, poses health hazards to humans.
Faculty of Veterinary Medicine, Department of Microbiology, Cairo University, Giza, Cairo, Egypt. s_mougy@hotmail.com
Kamelia
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Osman
Magdy
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Aly
Afaf
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Kheader
Khaled
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Mabrok
D000333:Aeromonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis* / Q000235:genetics; D002020:Buffaloes; D002162:Camelus; D002417:Cattle; D004534:Egypt; D008460:Meat / Q000382:microbiology*; D052899:Pore Forming Cytotoxic Proteins / Q000032:analysis* / Q000235:genetics; D015995:Prevalence; D012756:Sheep; D037521:Virulence Factors / Q000032:analysis* / Q000235:genetics
D001427:Bacterial Toxins; D052899:Pore Forming Cytotoxic Proteins; D037521:Virulence Factors; C032676:aerolysin