Neural progenitor and hemopoietic stem cells inhibit the growth of low-differentiated glioma.
The effects of neural progenitor and hemopoietic stem cells on C6 glioma cells were studied in in vivo and in vitro experiments. Considerable inhibition of proliferation during co-culturing of glioma cells with neural progenitor cells was revealed by quantitative MTT test and bromodeoxyuridine incorporation test. Labeled neural progenitor and hemopoietic stem cells implanted into the focus of experimental cerebral glioma C6 survive in the brain of experimental animals for at least 7 days, migrate with glioma cells, and accumulate in the peritumoral space. Under these conditions, neural progenitor cells differentiate with the formation of long processes. Morphometric analysis of glioma cells showed that implantation of neural progenitor and hemopoietic stem cells is accompanied by considerable inhibition of the growth of experimental glioma C6 in comparison with the control. The mechanisms of tumor-suppressive effects of neural and hemopoietic stem cells require further investigation.
Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Mitistry of Health Care and Social Development of the Russian Federation, Moscow, Russia. serpoff@gmail.com
V P
VP
Baklaushev
N F
NF
Grinenko
E A
EA
Savchenko
S N
SN
Bykovskaya
G M
GM
Yusubalieva
I V
IV
Viktorov
A S
AS
Bryukhovetskii
I S
IS
Bryukhovetskii
V P
VP
Chekhonin
D000818:Animals; D001854:Bone Marrow Cells; D001932:Brain Neoplasms / Q000473:pathology / Q000628:therapy*; D049109:Cell Proliferation; D002470:Cell Survival; D018920:Coculture Techniques; D005260:Female; D005910:Glioma / Q000473:pathology / Q000628:therapy*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D015552:Injections, Intralesional; D008856:Microscopy, Fluorescence; D009296:Nasal Cavity / Q000166:cytology; D009368:Neoplasm Transplantation; D058953:Neural Stem Cells / Q000166:cytology* / Q000637:transplantation; D009831:Olfactory Mucosa / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D016196:S Phase; D014183:Transplantation, Heterologous