Development and characterization of a novel human Waldenström macroglobulinemia cell line: RPCI-WM1, Roswell Park Cancer Institute - Waldenström Macroglobulinemia 1.
Understanding the biology of Waldenström macroglobulinemia is hindered by a lack of preclinical models. We report a novel cell line, RPCI-WM1, from a patient treated for WM. The cell line secretes human immunoglobulin M (h-IgM) with κ-light chain restriction identical to the primary tumor. The cell line has a modal chromosomal number of 46 and harbors chromosomal changes such as deletion of 6q21, monoallelic deletion of 9p21 (CDKN2A), 13q14 (RB1) and 18q21 (BCL-2), with a consistent amplification of 14q32 (immunoglobulin heavy chain; IgH) identical to its founding tumor sample. The clonal relationship is confirmed by identical CDR3 length and single nucleotide polymorphisms as well as a matching IgH sequence of the cell line and founding tumor. Both also harbor a heterozygous, non-synonymous mutation at amino acid 265 in the MYD88 gene (L265P). The cell line expresses most of the cell surface markers present on the parent cells. Overall, RPCI-WM1 represents a valuable model to study Waldenström macroglobulinemia.
Department of Hematology and Oncology, Mayo Clinic, Jacksonville, FL 32224, USA. chanan-khan.asher@mayo.edu
Kasyapa S
KS
Chitta
Aneel
A
Paulus
Sikander
S
Ailawadhi
Barbara A
BA
Foster
Michael T
MT
Moser
Petr
P
Starostik
Aisha
A
Masood
Taimur
T
Sher
Kena C
KC
Miller
Dan M
DM
Iancu
Jeffrey
J
Conroy
Norma J
NJ
Nowak
Sheila N
SN
Sait
David A
DA
Personett
Morton
M
Coleman
Richard R
RR
Furman
Peter
P
Martin
Stephen M
SM
Ansell
Kelvin
K
Lee
Asher A
AA
Chanan-Khan
D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D020732:Cytogenetic Analysis; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics / Q000378:metabolism; D016130:Immunophenotyping; D051379:Mice; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment; D014183:Transplantation, Heterologous; D008258:Waldenstrom Macroglobulinemia / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D053594:Myeloid Differentiation Factor 88