[Identification of sibling brothers using STR and Y-biallelic markers].
OBJECTIVE
To explore the methods for identification of sibling brothers with Y-STR locus mutation by detection of genetic markers on autosome and Y-biallelic.


METHODS
Goldeneye 20A and 18NC kit were used to genotyped the 35 STRs on autosome from two men. PowerPlex Y kit and Yfiler kit were used to genotyped the 16 STRs on Y chromosome full sibling index was calculated by ITO method. Twenty Y-biallelic markers were genotyped by fragment length discrepant allele specific PCR or general PCR.


RESULTS
Relationship of sibling brothers was found to have mutation of 2 loci on 16 Y-STR and the identical genetype of 20 Y-biallelic markers as well as a cumulative full sibling index of 4.3149 x 10(6) from 35 STRs on autosome.


CONCLUSIONS
In identification of paternal linage of Y-STR mutation, more genetic information can be acquired by detection of Y-biallelic markers including SNP and InDel.
Furong Forensic Science Identification Center of Hunan Second People's Hospital, Changsha 410007, China. tangmeiyun2000@yahoo.com.cn
Mei-yun
MY
Tang
Jian
J
Huang
Jin-hong
JH
Cai
Xiao-song
XS
Huang
Wei
W
Xu
Zhi-xiong
ZX
Qu
Fei-bao
FB
Shan
Hui-ling
HL
Lu
D000483:Alleles; D041322:Chromosomes, Human, Y / Q000235:genetics*; D053803:Forensic Genetics / Q000379:methods; D005787:Gene Frequency; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D035781:Siblings
D005819:Genetic Markers