Prediction of bladder cancer based on urinary content of MGEA5 and OGT mRNA level.
BACKGROUND
The mRNA expression of genes coding enzymes involved in O-GlcNAcylation were analyzed in urine obtained from 176 bladder cancer (BC) patients and 143 healthy persons.


METHODS
MGEA5 and OGT expression was measured by a real-time PCR assay.


RESULTS
OGT expression was not detected in urine of healthy persons but it was found in 51.7% of BC samples. Positive expression of MGEA5 was found in urine of both healthy persons (47.1%) and BC patients (52.3%). Poorly differentiated BC (grade III) showed significantly lower MGEA5 expression than grade I tumors. Contrary, OGT transcript level was significantly higher in grade II and III in comparison to grade I BC. Moreover, there was significant difference in OGT expression between early bladder cancers and invasive or advanced bladder cancers.


CONCLUSIONS
These results suggest that analysis of urinary content of MGEA5 and OGT may be useful for bladder cancer diagnostics.
2nd Department of Urology, Medical University of Lodz, Lodz, Poland.
Waldemar
W
Rozanski
Anna
A
Krzeslak
Ewa
E
Forma
Magdalena
M
Brys
Mariusz
M
Blewniewski
Paweł
P
Wozniak
Marek
M
Lipinski
D000368:Aged; D000951:Antigens, Neoplasm / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D051548:Histone Acetyltransferases / Q000235:genetics*; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics*; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000652:urine*; D060888:Real-Time Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000201:enzymology / Q000235:genetics*
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D051548:Histone Acetyltransferases; D017351:N-Acetylglucosaminyltransferases; C449880:O-GlcNAc transferase; C115866:OGA protein, human; D006821:Hyaluronoglucosaminidase