[Cellulose acetate membrane electrophoresis CAE and Raman spectroscopy as a method identification of beta-glucans, used as biologically and therapeutically active biomaterials].
BACKGROUND
The formation of AGEs progressively increases with normal aging, even in the absence of disease (the pathogenesis of diabetes associated vascular disorders and neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease). However, they are formed at accelerated rates in age-related diseases. The polysaccharides might play a role in wound healing, both internally and externally, and also that they could play a role against inflammation and may lead to the production of better medicines to be used as supplements in cancer treatment.


OBJECTIVE
The acid hydrolysis was studied with H2SO4 at 80% concentration to determine the most effective procedure for total hydrolysis of beta-glucan. The standard of beta-glucans acid hydrolysate were compared for commercial oat and oatmeal, mushrooms: Pleurotus ostreatus, Fungus and yeast Saccharomyces cerevisiae.


METHODS
The following materials and reagents were used in the examination: reference beta-(1 --> 3)-(1 --> 6)-glucan, oat and oatmeal, mushrooms: Pleurotus ostreatus, Fungus and yeast Saccharomyces cerevisiae. The Raman spectra of the sample solutions (beta-glucan acid hydrolysates) were recorded on a MAGNA-IR 860 with FT-Raman accessory. Sample was irradiated with a 1064 nm line of the T10-8S Nd spectra-physics model: YAG laser and scattered radiation were collected at 180 degrees, using 4 cm(-1) resolution. The polysaccharide was hydrolyzed into component monosaccharides with 80% H2SO4 at 0 degrees C for 30 minutes and monosaccharide derivatives were subjected to electrophoresis, as in a ealier authors study, on a strip of cellulose acetate membrane (CA-SYS-MINI Cellulose Acetate Systems) in 0.2 M Ca(OAc)2 (pH 7.5) at 10 mA, max. 240 V for 1.5 h. The strips were stained with 0.5% toluidine blue in 3% HOAc solution and then rinsed in distilled water and air-dried.


CONCLUSIONS
A part of the hexoses (for example glucose) are converted, to products such as 5-hydroxymethylfurfural. Various coloured substances, through the Maillard reaction have been reported for saccharides. The resulting mono- and oligosaccharides were analysed by cellulose acetate membrane electrophoresis CAE and Raman spectroscopy. Individual bands or CAE spots were selected to monitor the sugar content in medical plant cell walls and to confirm the identity of the analysed sample: oat and oatmeal, mushrooms: Pleurotus ostreatus, Fungus and yeast Saccharomyces cerevisiae. The possibility of a taxonomic classification of products rich in cell-wall materials based on cellulose acetate membrane electrophoresis CAE and Raman spectroscopy for authentication and detection of adulteration of products are discussed.
WydziałNauk o Materiałach i Srodowisku, Instytut Inzynierii Tekstyliów i Materiałów Polimerowych, Akademia Techniczno-Humanistyczna w Bielsku-Białej. apielesz@ath.bielsko.pl
Anna
A
Pielesz
Włodzimierz
W
Biniaś
Jadwiga
J
Paluch
D000363:Agaricales / Q000737:chemistry; D018554:Avena / Q000737:chemistry; D001672:Biocompatible Materials / Q000032:analysis* / Q000737:chemistry; D004588:Electrophoresis, Cellulose Acetate; D006868:Hydrolysis; D020076:Pleurotus / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D047071:beta-Glucans / Q000032:analysis* / Q000737:chemistry
D001672:Biocompatible Materials; D047071:beta-Glucans