[HPLC-MS identification of degradation products of levofloxacin].
The study aims to identify the degradation products of levofloxacin by HPLC-MS. The degradation products of levofloxacin were chromatographed on Agilent Zorbax Extend-C18 column (250 mm x 4.6 mm, 5 microm). The mobile phase was 0.1% ammonium acetate solution (using methanoic acid to adjust to pH 3.5)-acetonitrile at the flow rate of 0.5 mL x min(-1) (gradient elution), the column temperature was 40 degrees C. Descarboxyl levofloxacin, desmethyl levofloxacin and levofloxacin N-oxide were identified through comparing with the standard spectrum and the results of mass spectrometry, i.e. m/z 318.2 was descarboxyl levofloxacin, m/z 348.2 was desmethyl levofloxacin, m/z 378.1 was levofloxacin-N-oxide. This method is simple, fast, accurate and suitable for the identification of degradation products of levofloxacin.
Shandong Provincial Institute for Food and Drug Control, Jinan 250101, China.
Wei-Jian
WJ
Wang
Tao
T
Li
Jun
J
Li
Qi
Q
Liu
Yuan-Chao
YC
Xie
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D064704:Levofloxacin; D013058:Mass Spectrometry / Q000379:methods; D015242:Ofloxacin / Q000032:analysis* / Q000737:chemistry*; D010782:Photolysis
D064704:Levofloxacin; D015242:Ofloxacin