Efficacy of mesotherapy in facial rejuvenation: a histological and immunohistochemical evaluation.
BACKGROUND
Mesotherapy, commonly known as "biorejuvenation" or "biorevitalization", is a technique used to rejuvenate the skin by means of a transdermal injection of a multivitamin solution and natural plant extracts that are thought to improve the signs of skin aging.


OBJECTIVE
This prospective study aimed to evaluate the clinical effect of mesotherapy applied to periorbital wrinkles and to quantitatively evaluate histological changes in the skin occurring in response to the same treatment.


METHODS
Six volunteers with Fitzpatrick skin types III or IV and Glogau class I-III wrinkles were subjected to a three-month course of mesotherapy injections in the periocular area (six sessions administered at two-week intervals). Standard photographs and skin biopsies were obtained from the treatment area at baseline, at the end of treatment, and at three months post-treatment. Quantitative evaluation of collagen types I, III, and VII, newly synthesized collagen, total elastin, and tropoelastin was performed using a computerized morphometric analysis.


RESULTS
The clinical evaluation of volunteers at baseline, end of treatment, and three months post-treatment revealed no significant differences. Histological and immunostaining analysis of collagen types I, III, and VII, newly synthesized collagen, total elastin, and tropoelastin showed no statistically significant changes (P > 0.05) after mesotherapy injection.


CONCLUSIONS
The present study indicates that mesotherapy for skin rejuvenation does not result in statistically significant histological changes or clinical improvement.
Department of Dermatology, Al-Minya University, Al-Minya, Egypt Moawad Skin Institute for Laser, Cairo, Egypt. m_domyati@hotmail.com
Moetaz
M
El-Domyati
Tarek S
TS
El-Ammawi
Osama
O
Moawad
Hasan
H
El-Fakahany
Walid
W
Medhat
Mỹ G
MG
Mahoney
Jouni
J
Uitto
D000328:Adult; D003094:Collagen / Q000032:analysis; D003357:Cosmetic Techniques; D004549:Elastin / Q000032:analysis; D005145:Face; D005260:Female; D006801:Humans; D057748:Mesotherapy / Q000379:methods*; D008875:Middle Aged; D012060:Rejuvenation; D015595:Skin Aging; D016896:Treatment Outcome
D003094:Collagen; D004549:Elastin