Suppression of CX43 expression by miR-20a in the progression of human prostate cancer.
The aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. The present study found miR-20a to be significantly upregulated in prostate cancer compared with normal prostate tissues. The proliferation and colony formation assays revealed that the downregulation of miR-20a by miR-20a inhibitor suppresses the proliferation of MDA-PCa-2b cells in vitro and also inhibits tumor growth in vivo. Furthermore, a gap junction protein, α 1 (CX43), was identified as a direct target gene of miR-20a. The upregulation of CX43 was detected in MDA-PCa-2b cells after treatment with miR-20a inhibitor both in vitro and in vivo. In conclusion, the findings show that miR-20a significantly contributes to the progression of prostate cancer by targeting CX43.
Urologic Institute of PLA, Southwest Hospital, Third Military Medical University, Chongqing, China.
Xin
X
Li
Jin-Hong
JH
Pan
Bo
B
Song
En-Qing
EQ
Xiong
Zhi-Wen
ZW
Chen
Zhan-Song
ZS
Zhou
Yong-Ping
YP
Su
D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D018031:Connexin 43 / Q000235:genetics* / Q000378:metabolism; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D023041:Xenograft Model Antitumor Assays
D018031:Connexin 43; C534451:MIRN20a microRNA, human; D035683:MicroRNAs