Preparative isolation and purification of four compounds from Cistanches deserticola Y.C. Ma by high-speed counter-current chromatography.
Following a constituent enrichment step on a silica gel column, four phenyl-ethanoid glycosides were successfully isolated from Cistanches deserticola and purified by preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (40:6:6:50, v/v/v/v). A total of 30.9 mg acteoside, 13.0 mg isoacteoside, 12.5 mg syringalide A 3'-α-L-rhamnopyranoside and 7.2 mg 2'-acetylacteoside with purity of higher than 95%, as determined by HPLC-ELSD, were obtained in one-step separation from 297 mg of Cistanche deserticola extract, respectively. Their structures were identified by HR-MS, ¹H-NMR and ¹³C-NMR.
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Tianjin 300193, China.
Lifeng
L
Han
Lina
L
Ji
Mavis
M
Boakye-Yiadom
Wei
W
Li
Xinbo
X
Song
Xiumei
X
Gao
D002851:Chromatography, High Pressure Liquid; D031671:Cistanche / Q000737:chemistry*; D045424:Complex Mixtures / Q000032:analysis; D003377:Countercurrent Distribution / Q000379:methods*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry
D045424:Complex Mixtures; D006027:Glycosides; D012997:Solvents