Biochemical response to exposure to six textile dyes in early developmental stages of Xenopus laevis.
The present study was undertaken to determine the toxic effect of a lethal concentration of six different commercially used textile dyes on the 46th stage of Xenopus laevis tadpoles. The tadpoles were exposed to Astrazon Red FBL, Astrazon Blue FGRL, Remazol Red RR, Remazol Turquoise Blue G-A, Cibacron Red FN-3G, and Cibacron Blue FN-R for 168 h in static test conditions, and thus, 168-h median lethal concentrations (LC(50)s) of each dye were determined to be 0.35, 0.13, 112, 7, 359, and 15.8 mg/L, respectively. Also, to evaluate the sublethal effects of each dye, tadpoles were exposed to different concentrations of dyes (with respect to 168-h LC(50)s) for 24 h. The alteration of selected enzyme activities was tested. For this aim, glutathione S-transferase (GST), carboxylesterase, and lactate dehydrogenase (LDH) were assayed. After dye exposure, the GST induction or inhibition and LDH induction indicated some possible mechanisms of oxidative stress and deterioration in aerobic respiration processes induced by the tested dyes. Findings of the study suggest that selected biomarker enzymes are useful in understanding the toxic mechanisms of these dyes in X. laevis tadpoles as early warning indicators. Therefore, these selected biomarkers may evaluate the effect of environmental factors, such as textile dye effluents and other industrial pollutants, on amphibians in biomonitoring studies.
Laboratory of Environmental Toxicology, Department of Biology, Faculty of Arts and Science, Inonu University, 44280 Malatya, Turkey.
Abbas
A
Güngördü
Ayse
A
Birhanli
Murat
M
Ozmen
D000818:Animals; D001391:Azo Compounds / Q000633:toxicity; D043182:Carboxylesterase / Q000378:metabolism; D004396:Coloring Agents / Q000633:toxicity*; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism; D007814:Larva / Q000187:drug effects; D008297:Male; D018570:Risk Assessment; D013784:Textiles; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014982:Xenopus laevis
D001391:Azo Compounds; D004396:Coloring Agents; D014874:Water Pollutants, Chemical; D005982:Glutathione Transferase; D043182:Carboxylesterase