[Detection of ESBL-positive strains of Escherichia coli in pigs in the Czech Republic].
BACKGROUND
The goals of the presented regional study were monitoring the prevalence of Enterobacteriaceae producing broad-spectrum beta-lactamases in slaughter pigs and their basic molecular biology analysis.


METHODS
In the presented study, rectal swabs from 118 slaughter pigs from 5 farms in the Olomouc Region were analyzed. Bacteriological tests aimed at detection and identification of bacteria from the Enterobacteriaceae family producing broad-spectrum beta-lactamases. Suspected isolates were further analyzed using both phenotypic and genotypic methods.


RESULTS
From the group of 118 analyzed samples, seven Escherichia coli strains with the presence of the bla genes encoding CTX-M-1 beta-lactamases were isolated. Genes for TEM and SHV enzymes were not detected. This is the first report of ESBL-positive isolates of Escherichia coli in pigs in the Czech Republic.
State Veterinary Institute in Olomouc, Czech Republic. jbardon@svul.cz
J
J
Bardoň
V
V
Husičková
M
M
Chromá
M
M
Kolář
D000818:Animals; D018153:Czech Republic; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012007:Rectum / Q000382:microbiology; D034421:Sus scrofa / Q000382:microbiology*; D001618:beta-Lactamases / Q000096:biosynthesis*
D001618:beta-Lactamases