[Determination of brodifacoum in rat plasma by HPLC].
OBJECTIVE
A determination method of brodifacoum in rat plasma with bromadiolone as an internal standard was developed.


METHODS
A volume of 10 microl internal standard (bromadiolone) was added into rat plasma, and then extracted by 0.5 ml of acetonitrile by shaking for 2 min. The residue was dissolved with 200 microl of mobile phase after centrifugation for 10 min, and evaporation to dryness by Nitrogen blowing. A C18 column and PDA detector were used for separating and detecting. The wavelength was 254 nm, the flow rate was 1.0 ml/min, and the injection volume was 20 microl.


RESULTS
The liner range was 1.0-20 microg/ml, and the correlation coefficient was 0.9992. The detection limit was 0.3 microg/ml in plasma (S/N=3). The intra-assay and inter-assay coefficients of variation were 1.89%-2.45% and 2.51%-3.61% respectively. The recoveries in plasma at levels of low, middle and high concentrations were (80.8 +/- 3.1)%, (81.8 +/- 2.7)% and (87.9 +/- 3.6)% (n=6), respectively. The accuracies were 84.1%-91.5% and 86.7%-93.2%, respectively.


CONCLUSIONS
This method is simple, fast and accurate for the determination of brodifacoum in rat plasma.
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing.
Zhao-hui
ZH
Fu
Quan-kai
QK
Wang
Wen-lan
WL
Yu
Jian
J
Wang
An-na
AN
Wang
Jian-ning
JN
Xu
D015110:4-Hydroxycoumarins / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D010949:Plasma / Q000737:chemistry*; D051381:Rats
D015110:4-Hydroxycoumarins; C021636:bromadiolone