[Re-mineralization ability of Galla chinensis extracts on root carious lesions with or without non-collagen proteins].
OBJECTIVE
To evaluate the re-mineralization ability of Galla Chinensis extracts (GCE) on two artificial carious lesions in bovine root de-mineralized in vitro.


METHODS
Fourteen bovine root blocks were divided into two parts from buccal to lingual direction. The mesial blocks were treated with a demineralization solution and the distant blocks were treated with another demineralization solution. Two specimens from each group were selected randomly and examined with polarization microscope (PLM). After all blocks were demineralized, half surface of the demineralized zone was covered and the another half was treated with 0.5% NaCl to extract soluble dentin phosphate protein (S-DPP). Then all specimens were submitted to pH-cycling for one week. In the first four days, all specimens were treated with GCE for 21 h and with demineralization solution for 3 h. In the remaining three days, all specimens were treated with GCE. The re-mineralization ability of GCE on the specimens was evaluated by laser scanning confocal microscope (LSCM).


RESULTS
There existed intact surface layers on subsurface lesions but no surface layers were produced on erosive lesions. The re-mineralization ability of GCE on erosive lesions improved significantly with the treatment of 0.5% NaCl solution (P < 0.05). But it had no significant effect on subsurface lessions.


CONCLUSIONS
Extraction of S-DPP with 0.5% NaCl can improve the re-mineralization ability of GCE on root caries with erosive lesions. This finding supports the proposition that Galla Chinesis may be a promising anti-caries natural medicine in the future.
Department of Operative Dentistry and Endodontics, Stomatology College of Tianjin Medical University, Tianjin 300070, China.
Ke-hua
KH
Que
Bin
B
Guo
Bo
B
Wang
Qian-qian
QQ
Liang
Hong-hui
HH
Xie
D000818:Animals; D002327:Cariostatic Agents / Q000627:therapeutic use*; D002417:Cattle; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use*; D016326:Extracellular Matrix Proteins / Q000302:isolation & purification; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000627:therapeutic use; D010750:Phosphoproteins / Q000302:isolation & purification; D017213:Root Caries / Q000188:drug therapy*; D012795:Sialoglycoproteins / Q000302:isolation & purification; D014089:Tooth Remineralization; D000099311:Dentin Sialophosphoprotein
D002327:Cariostatic Agents; D004365:Drugs, Chinese Herbal; D016326:Extracellular Matrix Proteins; D005707:Gallic Acid; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein