Comparative transcriptome analysis of cadmium responses in Solanum nigrum and Solanum torvum.
• Solanum nigrum is a cadmium (Cd) accumulator, whereas Solanum torvum is a low Cd-accumulating plant. The molecular mechanisms that are responsible for differential cadmium (Cd) accumulation in the two Solanum species are poorly understood. • Here, grafting experiments confirmed that increased Cd loading into the root xylem was responsible for the differential Cd accumulation in the two Solanum species. An iron (Fe) supply assay indicated that low Fe accumulation in S. torvum leaves is related to its Cd sensitivity. • Transcriptome analyses revealed higher expression of the genes that encode several metal transporters as well as antioxidant-related genes, and several organic and amino acid biosynthesis/metabolism-related genes in Cd-treated S. nigrum. Our data also indicated that the different responsive mechanisms of the transporter genes to Fe deficiency might be responsible for differential uptake and redistribution of metals in the two Solanum species • These results form a basis upon which to further explore the molecular mechanisms of Cd accumulation and tolerance, and provide an insight into novel strategies that can be used for phytoremediation and food safety.
Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong RD, Shijiazhuang 050021, China.|Hebei Province Engineering Laboratory for Plant Breeding and Germplasm Enhancement of Stress-Tolerant Plants, 286 Huaizhong RD, Shijiazhuang 050021, China.
Jin
J
Xu
Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong RD, Shijiazhuang 050021, China.
Jianhang
J
Sun
Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong RD, Shijiazhuang 050021, China.
Liguo
L
Du
Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong RD, Shijiazhuang 050021, China.|Hebei Province Engineering Laboratory for Plant Breeding and Germplasm Enhancement of Stress-Tolerant Plants, 286 Huaizhong RD, Shijiazhuang 050021, China.
Xiaojing
X
Liu
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D000975:Antioxidants / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002104:Cadmium / Q000633:toxicity*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D007501:Iron / Q000494:pharmacology; D018977:Micronutrients / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D032322:Solanum / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D032324:Solanum nigrum / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development
D000975:Antioxidants; D018977:Micronutrients; D010940:Plant Proteins; D002104:Cadmium; D007501:Iron