Fluorescence-labeled immunomicelles: preparation, in vivo biodistribution, and ability to cross the blood-brain barrier.
Multifunctional hybrid micelles are prepared from amphiphilic mal-PEG-b-PLA and mPEG-b-P(LA-co-DHC/RhB) block copolymers. A specific anti-transferrin receptor antibody, OX26, is linked onto the surface of the micelles. ELISA indicates that the conjugated antibody preserves its activity. OX26 conjugation can increase the uptake efficiency of micelles by target cell lines (C6). Pharmacokinetics and in vivo biodistribution experiments are carried out to investigate the ability of OX26-conjugated micelles (immunomicelles) to cross the blood-brain barrier. The data show that the brain uptake of OX26-conjugated micelles is much more than that of OX26-free ones. Therefore, OX26-conjugated micelles will be promising drug carriers to cross the blood-brain barrier.
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Jun
J
Yue
Shi
S
Liu
Rui
R
Wang
Xiuli
X
Hu
Zhigang
Z
Xie
Yubin
Y
Huang
Xiabin
X
Jing
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D051381:Rats; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D008823:Micelles