Protocol for the use of enzyme-linked hybridization assays for genital ulcer disease.
Etiologic agents of genital ulcer disease include herpes simplex 1 and 2, Treponema pallidum pallidum, Haemophilus ducreyi, and Klebsiella granulomatis. The advent of PCR has allowed for more rapid and sensitive detection of microbial pathogens. In this protocol, we describe the simultaneous detection of these five pathogens and an internal control using a single-tube multiplex PCR and colorimetric enzyme-linked amplicon hybridization assay.
Sir Albert Sakzewski Virus Research Centre, Herston, QLD, Australia. Seweryn@uq.edu.au
Seweryn
S
Bialasiewicz
Ian M
IM
Mackay
David M
DM
Whiley
Theo P
TP
Sloots
D004798:Enzymes / Q000378:metabolism*; D006191:Haemophilus ducreyi / Q000235:genetics / Q000302:isolation & purification; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification; D007709:Klebsiella / Q000235:genetics / Q000302:isolation & purification; D025202:Molecular Diagnostic Techniques; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D012749:Sexually Transmitted Diseases / Q000382:microbiology* / Q000821:virology*; D014210:Treponema pallidum / Q000235:genetics / Q000302:isolation & purification; D014456:Ulcer / Q000382:microbiology* / Q000821:virology*
D004798:Enzymes