[Effect of UC-MSCs on inflammation and thrombosis of the rats with collagen type II induced arthritis].
OBJECTIVE
To investigate the immunoregulation effects of umbilical cord mesenchymal stem cells (UC-MSCs) on the rats with collagen II induced arthritis (CIA).


METHODS
The rats were first immunized by intradermal injection of chicken collagen type II emulsified with complete Freund's adjuvant (CFA) to monitor their swelling of foot, hair color and action state. After injected UC-MSC by caudal vein, the rats were scored with the arthritis index (AI) once a week. Then, the concentration of interleukin (IL-6), tumor necrosis factor-α (TNF-α) in serum and D-dimer (D-D), antithrombin-III (AT-III), thrombomodulin (TM) in plasma were detected by ELISA.


RESULTS
Obvious swellings of the feet were found in the experiment group compared with normal one. ELISA analysis showed that the concentrations of IL-6, TNF-α, D-D and TM in plasma of the experiment group as of (200.48 ± 15.04) ng/L, (450.25 ± 45.39) ng/L, (274.26 ± 67.93) ng/L and (9.18 ± 0.84) µg/L, respectively were higher than of(167.62 ± 0.97) ng/L, (371.44 ± 21.26) ng/L, (193.95 ± 8.22) ng/L and (6.30 ± 0.32) µg/L respectively in normal group (P < 0.05), but the concentration of AT-III \[(89.57 ± 6.40) ng/L\] was lower than normal group \[(112.82 ± 1.74) ng/L\] (P < 0.05). The levels of cytokines through the UC-MSCs treatment were significantly different from the model group (P < 0.05). After 9 weeks, these cytokines in the UC-MSCs group were mostly the same as the normal group.


CONCLUSIONS
The thrombophilia status of the CIA rats was caused by immune injury. The UC-MSCs reduced the production of inflammatory cytokines and regulated and repaired the balance of coagulation and anticoagulation system of the body to cure the immune-related thrombophilia.
Clinical Medical College of Yangzhou University, Yangzhou Institute of Hematology, Yangzhou, Jiangsu, China.
Chuan-ming
CM
Lin
Jian
J
Gu
Yu
Y
Zhang
Lian-jun
LJ
Shen
Li
L
Ma
Jun
J
Ni
Zhong-qiang
ZQ
Wang
Wei
W
Wu
D000818:Animals; D000991:Antithrombins / Q000097:blood; D001169:Arthritis, Experimental / Q000276:immunology / Q000503:physiopathology / Q000517:prevention & control*; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D007249:Inflammation; D015850:Interleukin-6 / Q000097:blood; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013927:Thrombosis / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014470:Umbilical Cord / Q000166:cytology
D000991:Antithrombins; D005338:Fibrin Fibrinogen Degradation Products; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C036309:fibrin fragment D