Salivary proteome modifications associated with periodontitis in obese patients.
OBJECTIVE
To identify changes in the salivary protein/peptide profiles by differential proteomics in obese patients with or without periodontitis.


METHODS
Periodontal examinations and whole saliva samples were obtained from 38 obese patients (mean age: 45.1 ± 7.3 years, mean BMI: 49.3 ± 9 kg/m(2) ) including 13 periodontitis and 25 non-periodontitis subjects, and 19 healthy controls (mean age: 44.2 ± 6.4 years, mean BMI: 21.5 ± 2.1 kg/m(2) ). Surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS) was used to compare the whole saliva polypeptide profiles.


RESULTS
The SELDI-TOF-MS analysis detected eight putative markers. Six of them were increased and identified in obese subjects versus controls (albumin, α and β haemoglobin chains, α-defensins 1, 2 and 3). Alpha-defensins were less abundant in saliva of periodontitis obese patients (36.47 ± 19.84 μA) versus non-periodontitis obese patients (43.44 ± 30.34  μA), whereas α-defensins were more abundant in obese patients (40.99 ± 26.66  μA) versus controls (27.1 ± 23.98  μA).


CONCLUSIONS
Periodontal status modifies the salivary proteome in obese patients. Alpha-defensins may play a role in gingival inflammation, and be involved in the higher susceptibility of obese patients to periodontal diseases.
Department of Periodontology, Service of Odontology, Garancière Rothschild Hospital, AP-HP, Paris 7-Denis Diderot University, U.F.R. of Odontology, Paris, France.
Hélène
H
Rangé
Thibaut
T
Léger
Clarisse
C
Huchon
Cécile
C
Ciangura
Devy
D
Diallo
Christine
C
Poitou
Olivier
O
Meilhac
Philippe
P
Bouchard
Catherine
C
Chaussain
D000328:Adult; D000368:Aged; D000418:Albumins / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications* / Q000276:immunology / Q000378:metabolism; D010518:Periodontitis / Q000150:complications / Q000276:immunology / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D012016:Reference Values; D012463:Saliva / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000378:metabolism; D023084:alpha-Defensins / Q000378:metabolism*
D000418:Albumins; D006454:Hemoglobins; D020543:Proteome; D012471:Salivary Proteins and Peptides; D023084:alpha-Defensins