Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity.
The aim of this study was to produce and characterize nanoparticles (NPs), combining chondroitin sulfate (CS) and fucoidan (FC) with chitosan for therapeutic purposes. These NPs were characterized by dynamic light scattering, zeta potential determination, and transmission electronic microscopy. The anticoagulant activity was determined for FC NPs and compared with FC solution at the same concentration. FC NPs showed regular shapes and better anticoagulant activity than free polysaccharide solution. FC solution did not affect coagulation compared to FC NPs, which increased up to two-fold, even at a lower concentration. Cytotoxicity and permeability tests were conducted using Caco-2 cell monolayer, exhibiting no toxic effect in this cell line and higher permeability for NP2 samples than FC solution at the same concentration.
Drug Department, Rio de Janeiro Federal University, Rio de Janeiro, Brazil. lulaufrj@hotmail.com
Luiz Cláudio
LC
da Silva
Thiago
T
Garcia
Michela
M
Mori
Giuseppina
G
Sandri
Maria Cristina
MC
Bonferoni
Priscila V
PV
Finotelli
Leonardo P
LP
Cinelli
Carla
C
Caramella
Lúcio M
LM
Cabral
D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010314:Partial Thromboplastin Time; D010316:Particle Size; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology
D000925:Anticoagulants; D011134:Polysaccharides; D002809:Chondroitin Sulfates; D048271:Chitosan; C007789:fucoidan