FLO11 expression and lipid biosynthesis are required for air-liquid biofilm formation in a Saccharomyces cerevisiae flor strain.
Air-liquid biofilm formation is largely dependent on Flo11p and seems related to cell lipid content and composition. Here, it is shown that in the presence of cerulenin, a known inhibitor of the fatty acid synthase complex, biofilm formation is inhibited together with FLO11 transcription in a flor strain of Saccharomyces cerevisiae, while the administration of saturated fatty acids to cerulenin-containing medium restores biofilm formation and FLO11 transcription. It is also shown that, in biofilm cells, the FLO11 transcription is accompanied by the transcription of ACC1, ACS1 and INO1 key genes in lipid biosynthesis and that biofilm formation is affected by the lack of inositol in flor medium. These results are compatible with the hypothesis that the air-liquid biofilm formation depends on FLO11 transcription levels as well as on fatty acids biosynthesis.
Dipartimento di Agraria, Università di Sassari, Sassari, Italy.
Giacomo
G
Zara
Paola
P
Goffrini
Tiziana
T
Lodi
Severino
S
Zara
Ilaria
I
Mannazzu
Marilena
M
Budroni
D000391:Air Microbiology; D018441:Biofilms / Q000254:growth & development*; D002569:Cerulenin / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000378:metabolism; D020869:Gene Expression Profiling; D050356:Lipid Metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014871:Water Microbiology
D003470:Culture Media; D004791:Enzyme Inhibitors; C110690:FLO11 protein, S cerevisiae; D008562:Membrane Glycoproteins; D029701:Saccharomyces cerevisiae Proteins; D002569:Cerulenin