Evaluating the efficacy of an avian-specific probiotic to reduce the colonization of Campylobacter jejuni in broiler chickens.
Campylobacteriosis is the most frequent zoonotic disease in humans worldwide, and the contaminated poultry meat by Campylobacter jejuni can be considered one of the important sources of enteric infections in humans. The use of probiotics, which can help to improve the natural defense of animals against pathogenic bacteria, is an alternative and effective approach to antibiotic administration for livestock to reduce bacterial contamination. In vitro experiments showed that Enterococcus faecium, Pediococcus acidilactici, Lactobacillus salivarius, and Lactobacillus reuteri isolated from healthy chicken gut inhibited the growth of C. jejuni. To demonstrate this effect in vivo, 1-d-old broiler chicks received 2 mg/bird per day of a multispecies probiotic product via the drinking water. Controls received no probiotic treatment, and all chicks were infected with C. jejuni orally. Results showed that the cecal colonization by C. jejuni was significantly reduced by probiotic treatment at both 8 and 15 d postchallenge. To confirm this effect, in a second in vivo experiment, 1-d-old broiler chicks received the same dose of the same probiotic via the drinking water and controls received no probiotic, and all chicks were infected with C. jejuni orally. Similarly, probiotic treatment reduced (P=0.001) cecal colonization by C. jejuni at both 8 and 15 d postchallenge. The results of our in vivo experiments conclude that probiotic administration reduced the colonization of C. jejuni in broiler chickens.
Institute of Animal Nutrition, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.
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K
Ghareeb
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Awad
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Mohnl
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Porta
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Biarnés
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Böhm
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Schatzmayr
D000818:Animals; D002169:Campylobacter Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D016123:Campylobacter jejuni; D002645:Chickens / Q000382:microbiology*; D004333:Drug Administration Routes; D005260:Female; D008297:Male; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D019936:Probiotics / Q000494:pharmacology*; D014871:Water Microbiology