Alkaloids from Pachysandra terminalis inhibit breast cancer invasion and have potential for development as antimetastasis therapeutic agents.
The aim of the present study was to identify potentially useful natural compounds for the development of novel therapeutic agents to inhibit metastasis. A phytochemical investigation of Pachysandra terminalis resulted in the isolation of seven new pregnane alkaloids, terminamines A-G (1-7), and seven known alkaloids (8-14). The structures of 1-7 were elucidated by 1D- and 2D-NMR spectroscopic and mass spectrometric methods. Compounds 1-5 and 8-14 inhibited the migration of MB-MDA-231 breast cancer cells induced by the chemokine epithelial growth factor. In addition, compound 1 inhibited phosphorylation of integrin β(1), which plays an important role in MB-MDA-231 cell adhesion and metastasis.
Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy,  Tianjin Medical University , Tianjin 300070, People's Republic of China.
Hui-Yuan
HY
Zhai
Chuan
C
Zhao
Ning
N
Zhang
Mei-Na
MN
Jin
Sheng-An
SA
Tang
Nan
N
Qin
De-Xin
DX
Kong
Hong-Quan
HQ
Duan
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005260:Female; D006801:Humans; D015394:Molecular Structure; D009362:Neoplasm Metastasis / Q000188:drug therapy; D019906:Nuclear Magnetic Resonance, Biomolecular; D031236:Pachysandra / Q000737:chemistry*; D011278:Pregnanes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
D000470:Alkaloids; D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D011278:Pregnanes