In vitro degradation of three-dimensional chitosan/apatite composite rods prepared via in situ precipitation.
Three-dimensional (3D) compact rods with multilayer structure made from chitosan (CHI) and apatite (Ap) have been prepared. The cytocompatibility assay revealed that the CHI/Ap composite could promote cell proliferation. In vitro degradation behaviors of the rods have been systematically investigated for up to 6 weeks in phosphate buffer saline (PBS) solution at 37°C. The properties of the composite rods were measured by means of weight loss, swelling ratio, and the changes in mechanical properties, etc. The pH of the PBS solution during the first 2 weeks of degradation was also detected. Results showed that the medium of CHI/Ap composite rods exhibited more stable pH change compared with that of CHI rods. Weight loss as well as the changes in mechanical properties happened more often to CHI rods than CHI/Ap rods. The presence of Ap could effectively reduce the degradation rate of the composite rods. All the results suggested that the composite rods could keep the initial shapes and mechanical properties longer than the pure CHI rods.
Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
Xi-ming
XM
Pu
Qing-qing
QQ
Yao
Yun
Y
Yang
Zhen-zhen
ZZ
Sun
Qi-qing
QQ
Zhang
D016475:3T3 Cells; D000818:Animals; D001031:Apatites / Q000737:chemistry*; D002021:Buffers; D011232:Chemical Precipitation; D048271:Chitosan / Q000737:chemistry* / Q000302:isolation & purification* / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D055595:Mechanical Phenomena; D051379:Mice; D008970:Molecular Weight; D010710:Phosphates / Q000737:chemistry; D014867:Water / Q000737:chemistry
D001031:Apatites; D002021:Buffers; D010710:Phosphates; D014867:Water; D048271:Chitosan