3q26 amplification is an effective negative triage test for LSIL: a historical prospective study.
BACKGROUND
Women with low grade squamous intraepithelial lesions (LSIL) at cervical cancer screening are currently referred for further diagnostic work up despite 80% having no precancerous lesion. The primary purpose of this study is to measure the test characteristics of 3q26 chromosome gain (3q26 gain) as a host marker of carcinogenesis in women with LSIL. A negative triage test may allow these women to be followed by cytology alone without immediate referral to colposcopy.


RESULTS
A historical prospective study was designed to measure 3q26 gain from the archived liquid cytology specimens diagnosed as LSIL among women attending colposcopy between 2007 and 2009. 3q26 gain was assessed on the index liquid sample; and sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were measured at immediate triage and at 6-16 months after colposcopic biopsy. The sensitivity of 3q26 gain measured at immediate triage from automated and manually reviewed tests in 65 non-pregnant unique women was 70% (95% CI: 35, 93) with a NPV of 89% (95% CI: 78, 96). The sensitivity and NPV increased to 80% (95% CI: 28, 99) and 98% (95% CI: 87, 100), respectively, when only the automated method of detecting 3q26 gain was used.


CONCLUSIONS
3q26 gain demonstrates high sensitivity and NPV as a negative triage test for women with LSIL, allowing possible guideline changes to routine surveillance instead of immediate colposcopy. Prospective studies are ongoing to establish the sensitivity, specificity, PPV and NPV of 3q26 gain for LSIL over time.
Department of Biomedical and Health Informatics, University of Missouri-Kansas City School of Medicine, Kansas City, Missouri, United States of America.
Erica R
ER
Heitmann
Kamani M
KM
Lankachandra
Jeff
J
Wall
George D
GD
Harris
Hollie J
HJ
McKinney
G Reza
GR
Jalali
Yogita
Y
Verma
Eric
E
Kershnar
Michael W
MW
Kilpatrick
Petros
P
Tsipouras
Diane M
DM
Harper
D000328:Adult; D002893:Chromosomes, Human, Pair 3; D003127:Colposcopy; D005260:Female; D005784:Gene Amplification; D006801:Humans; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014218:Triage; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000235:genetics*