Type III apparatus of Pseudomonas aeruginosa as a tool to diagnose pulmonary infection in cystic fibrosis patients.
Pseudomonas aeruginosa is associated with increased mortality in cystic fibrosis (CF) patients, and expresses type III secretion system proteins (TTSP), which is a common mechanism used by gram-negative pathogens for delivery of anti-host factors. Our aim was to investigate whether or not these antigens (TTSP) would be recognized by CF sera, by Western blot reaction. We have showed herein that all patients (n = 11) not chronically infected by P. aeruginosa had their first serum positive for TTSP (ExoS, ExoT, PopB, and/or PopD). All chronic patients had a strong positive serology to TTSP, although relatively weak reactions to TTSP were observed for some individuals in the negative control group. Therefore, TTSP that were early produced in P. aeruginosa infected CF patients, induced a detectable antibody response in those patients and were easily detected by Western-blot reaction.
Department of Microbiology, Immunology and Parasitology, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Aline C
AC
Cruz
Bianca C
BC
Neves
Laurinda Y S
LY
Higa
Tânia
T
Folescu
Elizabeth A
EA
Marques
Lucimar G
LG
Milagres
D000293:Adolescent; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology / Q000378:metabolism; D001426:Bacterial Proteins / Q000097:blood / Q000276:immunology / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D003550:Cystic Fibrosis / Q000097:blood / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D011552:Pseudomonas Infections / Q000097:blood / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification* / Q000378:metabolism; D012141:Respiratory Tract Infections / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D012189:Retrospective Studies
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins