Effect of chemical and microbial vitamin B₁₂ analogues on production of vitamin B₁₂.
Strain improvement by genetic manipulation or optimization of fermentation conditions for overproduction of vitamin B(12) has a drawback due to feed back inhibition. To resist the feed back inhibition by analogues of vitamin B(12) in Propionibacterium freudenrechii subsps. shermanii (OLP-5), we have tested with microbially separated B(12) analogues from three different strains. Microbial analogues were differentiated from commercially available vitamin B(12) by high pressure liquid chromatography and spectrophotometric method. An analogue isolated from NRRL-B-4327 was shown to increase vitamin B(12) concentration from 18.53 ± 0.15 to 31.67 ± 0.58 mg/l in OLP-5 strain. The presence of chemical analogue (ICH(2) Co(DH)(2) (H(2)Py)(4)) increased vitamin B(12) production from 16.13 ± 0.15 to 18.53 ± 0.15 mg/l in OLP-5. These findings revealed that addition of B(12) analogues in fermentation media have developed strain resistance to feed back inhibition by vitamin B(12).
Department of Microbiology, Osmania University, Hyderabad 500007, Andhra Pradesh, India.
Yeruva
Y
Thirupathaiah
Chiliveri
C
Swarupa Rani
Marrivada
M
Sudhakara Reddy
Linga
L
Venkateswar Rao
D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D025461:Feedback, Physiological; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D011424:Propionibacterium / Q000235:genetics* / Q000378:metabolism*; D013053:Spectrophotometry; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000737:chemistry
D003470:Culture Media; D014805:Vitamin B 12