Beneficial effect of anti-interleukin-33 on the murine model of allergic inflammation of the lower airway.
OBJECTIVE
Interleukin (IL)-33, which mediates the T(h)2 allergic pathway, may play a key role in allergic airway inflammation. This study was conducted to evaluate the therapeutic potential of anti-IL-33 antibody for treatment of allergic inflammation of the lower airway in a murine model.


METHODS
Twenty-four BALB/c mice were used in this study. Saline was used for sensitization and challenge of mice in Group A (control group, n = 6). Mice in Group B (ovalbumin (OVA) group, n = 6) received intraperitoneal (ip) and intranasal OVA challenge. In Group C (control IgG group, n = 6), mice received ip injection with control IgG prior to OVA challenge. Mice in Group D (anti-IL-33 group, n = 6) received an ip injection of anti-IL-33 prior to challenge. Measurements of serum total and OVA-specific IgE and the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid were performed. We performed histopathologic examination to evaluate the degree of eosinophilic infiltration in lung tissue. Airway hyperreactivity was measured according to change of enhanced pause (Penh).


RESULTS
A significant decrease in serum total and OVA-specific IgE and the number of eosinophils and neutrophils in BAL fluid was observed in Group D, compared with Group B or Group C (p < .05). In Group D, treatment with anti-IL-33 resulted in a significant decrease in eosinophilic infiltration in lung tissue, compared with Group B and Group C (p < .05). Degree of airway hyperreactivity, measured by Penh, showed a significant decrease in the anti-IL-33 treatment group, compared with the OVA group or the control IgG treatment group (p < .01, at 50 mg/mL of methacholine).


CONCLUSIONS
Anti-IL-33 has therapeutic potential for treatment of allergic inflammation of the lower airway.
Department of Otorhinolaryngology, INHA University College of Medicine ,  Incheon ,  Republic of Korea.
Young Hyo
YH
Kim
Chang-Shin
CS
Park
Dae Hyun
DH
Lim
Sung-Hye
SH
Ahn
Byong Kwan
BK
Son
Jeong Hee
JH
Kim
Tae Young
TY
Jang
D000818:Animals; D000906:Antibodies / Q000627:therapeutic use; D001249:Asthma / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D016535:Bronchial Hyperreactivity / Q000209:etiology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004804:Eosinophils / Q000502:physiology; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D000067596:Interleukin-33; D007378:Interleukins / Q000037:antagonists & inhibitors* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology
D000906:Antibodies; C506535:Il33 protein, mouse; D000067596:Interleukin-33; D007378:Interleukins; D007073:Immunoglobulin E; D010047:Ovalbumin