Evaluation of oxidative stress among coronary diabetics patients.
OBJECTIVE
Determination of the superoxide dismutase (SOD), glutathione peroxidase (GPX) and the total antioxidant status (TAS) and evaluation of inflammation by the use of high sensitivity C reactive protein (hs-CRP) among Tunisian coronary diabetic patients.


METHODS
We measured the erythrocyte GPX activity and the plasmatic TAS concentration by colorimetric methods, the apolipoproteins [ApoA1, ApoB], hs-CRP and the fibrinogen by immunonephelometry assays.


RESULTS
TAS and GPX were significantly decreased among patients compared to the controls [TAS: 1,14 +/- 0,28 mmol/l vs 1,55 +/- 0,35 mmol/l, GPX: 59,32 +/- 10,72 U/gHb vs 149,19 +/- 30,95 U/gHb]. For the coronary diabetic patients, the TAS is correlated positively with hs-CRP [r= 0,01, p<10(-3)]. Pearson's correlation shows a significantly positive correlation between GPX and TAS among all patients.


CONCLUSIONS
Determination of antioxidative defense markers contributes to understanding the effect of stress oxidative on the development, prevention and therapy of cardiovascular disease. (www.actabiomedica.it).
Department of Clinical Biochemistry CHU-Farhat Hached Sousse, Tunisia. azzadandana@yahoo.fr
Azza
A
Dandana
Salima
S
Ferchichi
Faouzi
F
Addad
Zaineb
Z
Jaidane
Hinda
H
Chahed
Imen
I
Gammoudi
Abdelkader
A
Chalghoum
Giampiero
G
Bricca
Abdelhedi
A
Miled
D000368:Aged; D002097:C-Reactive Protein / Q000032:analysis; D002318:Cardiovascular Diseases / Q000378:metabolism*; D003124:Colorimetry; D003925:Diabetic Angiopathies / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism
D002097:C-Reactive Protein; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase