Protein conformational studies for macromolecularly imprinted polymers.
CD is used to clearly show the negative impact of common ligands on the overall conformation of BSA, a typical protein template in macromolecularly imprinted polymers. This change occurs at concentrations far lower than those generally used in the literature. These findings are important as they offer insight into a potential fundamental reason for the lack of success in protein imprinting to date despite significant interest from the scientific community.
Fletcher Stuckey Pratt Chair in Engineering, Departments of Chemical Engineering and Biomedical Engineering, University of Texas at Austin, 1 University Station C0400, Austin, TX 78712-1062, USA.
David R
DR
Kryscio
Michael Q
MQ
Fleming
Nicholas A
NA
Peppas
D000178:Acrylamides / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry; D000818:Animals; D001897:Boronic Acids / Q000737:chemistry; D002417:Cattle; D002942:Circular Dichroism; D003432:Cross-Linking Reagents / Q000737:chemistry; D008024:Ligands; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D000178:Acrylamides; D000179:Acrylates; D001897:Boronic Acids; D003432:Cross-Linking Reagents; D008024:Ligands; D011108:Polymers; D012710:Serum Albumin, Bovine; C028592:3-aminobenzeneboronic acid