Phenolic compound production in relation to differentiation in cell and tissue cultures of Larrea divaricata (Cav.).
The lignan nordihydroguaiaretic acid (NDGA) and its derivatives existing in Larrea divaricata species show a wide range of pharmacological activities which makes this genus an interesting target to consider the plant in vitro cultivation systems as a feasible alternative source for their production. These compounds are potentially useful in treating diseases related to heart condition, asthma, arteriosclerosis, viral and bacterial infections, inflammation and cancer. In the present study, calli, cell suspension cultures, and in vitro and wild plants of L. divaricata were investigated for their potential to synthesize phenolic compounds. Calli, both with and without organogenesis, produced NDGA and quercetin, as did plantlet and wild plants. NDGA was also produced by the cell suspension cultures, together with p-coumaric acid, ferulic acid and sinapyl alcohol. The capacity of undifferentiated tissues to form phenolic compounds is very limited, but when the calli underwent organogenesis, developing mainly adventitious shoots, the phenolic compound production increased significantly. Plantlets regenerated from adventitious shoots of L. divaricata calli did not show the same phenolic pattern as wild plants, with levels of NDGA and quercetin being 3.6- and 5.9-fold lower, respectively.
Unidad de Biotecnología, Centro de Excelencia en Productos y Procesos (CEPROCOR), Gobierno de la Provincia de Córdoba, Sede Santa María de Punilla: Pabellón CEPROCOR (X5164), Córdoba, Argentina. Electronic address: loren.palacio@gmail.com.
Lorena
L
Palacio
Departamento de Biología Agrícola, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Ruta nacional 36 km 601 (X5804BYA), Río Cuarto, Córdoba, Argentina.
Juan J
JJ
Cantero
Cátedra de Fisiología Vegetal, Facultad de Farmacia, Universidad de Barcelona, Av. Diagonal 643, CP 08028 Barcelona, Spain.
Rosa M
RM
Cusidó
Unidad de Biotecnología, Centro de Excelencia en Productos y Procesos (CEPROCOR), Gobierno de la Provincia de Córdoba, Sede Santa María de Punilla: Pabellón CEPROCOR (X5164), Córdoba, Argentina.
Marta E
ME
Goleniowski
D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D003373:Coumaric Acids / Q000378:metabolism; D032442:Larrea / Q000166:cytology* / Q000378:metabolism*; D009637:Masoprocol / Q000378:metabolism*; D010666:Phenylpropionates / Q000378:metabolism; D018520:Plant Shoots / Q000166:cytology* / Q000378:metabolism*; D010946:Plants, Medicinal / Q000166:cytology / Q000378:metabolism; D011794:Quercetin / Q000096:biosynthesis*
D003373:Coumaric Acids; D010666:Phenylpropionates; D009637:Masoprocol; C496130:sinapyl alcohol; D011794:Quercetin; C004999:ferulic acid