Chitosan and Chitin Hexamers affect expansion and differentiation of mesenchymal stem cells differently.
Chitooligosaccharides are of interest as potential drugs due to their bioactivity and water solubility. We compared the effect of acetylated and deacetylated chitooligomers (Hexamers) on short-term expansion (7 days) and osteogenic differentiation of bone-marrow derived, human mesenchymal stem cells in terms of gene expression, cytokine secretion and quality of osteogenic differentiation. We show that chitooligomers affect hMSC gene expression and cytokine secretion, but not mineralization. The effect of chitooligomers was shown to be dependent on the acetylation degree, with significantly stronger effects when cells are stimulated with chitin-derived Hexamers (N-Acetyl Chitohexaose) than with Chitosan Hexamers (Chitohexaose).
The Blood Bank, Landspitali University Hospital, Snorrabraut 60, 105 Reykjavik, Iceland.
Ramona
R
Lieder
Finnbogi
F
Thormodsson
C-H
CH
Ng
Jon M
JM
Einarsson
Johannes
J
Gislason
Petur H
PH
Petersen
Olafur E
OE
Sigurjonsson
D054392:Adipokines / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D000071451:Chitinase-3-Like Protein 1; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D058105:Polymerization; D013997:Time Factors; D051196:Toll-Like Receptor 3 / Q000378:metabolism
D054392:Adipokines; D001672:Biocompatible Materials; D015415:Biomarkers; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1; D016207:Cytokines; D037102:Lectins; C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3; D048271:Chitosan