The primary structure identification of a corn peptide facilitating alcohol metabolism by HPLC-MS/MS.
The aim of this study is to identify the primary structure of corn peptides (CPs) with a facilitating alcohol metabolism effect. Corn protein was hydrolyzed by Alcalase first. The hydrolysate, crude corn peptides (CPs), was then fractionated through ultrafiltration technology. The primary structure of a peptide from the fraction (Mm<5kDa) was identified by HPLC-MS/MS, coupled with the peptide sequence retrieval using the MS-MS online database. The amino acid sequence of the peptide was determined as Q-L-L-P-F, and the pentapeptide was synthesized by Fmoc solid-phase peptide synthesis (SPPS) method. Its ability to facilitate alcohol metabolism was evaluated in vivo. Results showed that the synthetic peptide (10mg/kg) had a higher ability to eliminate alcohol in vivo compared to the mixed peptides (Mm<5kDa, 200mg/kg). In conclusion, the pentapeptide Q-L-L-P-F has a potent ability in facilitating alcohol metabolism, and this pentapeptide is the main bioactive component in the mixed peptides obtained from corn.
College of Food Science and Technology, Huazhong Agricultural University & Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430070, People's Republic of China.
Zhi-Li
ZL
Ma
Wen-Jun
WJ
Zhang
Guo-Cai
GC
Yu
Hui
H
He
Yan
Y
Zhang
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000493:pharmacokinetics*; D005449:Fluorenes / Q000737:chemistry; D008658:Inactivation, Metabolic; D008297:Male; D008657:Metabolic Clearance Rate / Q000187:drug effects; D051379:Mice; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D010940:Plant Proteins / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D020539:Sequence Analysis, Protein; D053719:Tandem Mass Spectrometry; D003313:Zea mays
D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010446:Peptide Fragments; D010940:Plant Proteins; D000431:Ethanol