Aerobic degradation of 3-chlorobenzoic acid by an indigenous strain isolated from a polluted river.
An indigenous strain of Pseudomonas putida capable of degrading 3-chlorobenzoic acid as the sole carbon source was isolated from the Riachuelo, a polluted river in Buenos Aires. Aerobic biodegradation assays were performed using a 2-l microfermentor. Biodegradation was evaluated by spectrophotometry, chloride release, gas chromatography and microbial growth. Detoxification was evaluated by using Vibrio fischeri, Pseudokirchneriella subcapitata and Lactuca sativa as test organisms. The indigenous bacterial strain degrades 100 mg l(-1) 3-chlorobenzoic acid in 14 h with a removal efficiency of 92.0 and 86.1% expressed as compound and chemical oxygen demand removal, respectively. The strain was capable of degrading up to 1,000 mg of the compound l(-1). Toxicity was not detected at the end of the biodegradation process. Besides initial concentration, the effect of different factors, such as initial pH, initial inoculum, adaptation to the compound and presence of other substrates and toxic related compounds, was studied.
Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina. agallego@ffyb.uba.ar
Alfredo
A
Gallego
Virginia L
VL
Gemini
Ariana A
AA
Rossen
Susana L
SL
Rossi
Valeria
V
Trípodi
Daniel
D
Corach
Estela
E
Planes
Sonia E
SE
Korol
D000332:Aerobiosis; D048908:Aliivibrio fischeri / Q000187:drug effects; D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D002723:Chlorobenzoates / Q000378:metabolism* / Q000633:toxicity; D000460:Chlorophyta / Q000187:drug effects; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D018545:Lactuca / Q000187:drug effects; D016958:Pseudomonas putida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013053:Spectrophotometry; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity
D002723:Chlorobenzoates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; C036427:3-chlorobenzoic acid; D002244:Carbon