Immunophenotypic characterization and distribution of dendritic cells in odontogenic cystic lesions.
OBJECTIVE
To analyze the expression and distribution patterns of mature dendritic cells (mDCs) and immature DCs (imDCs) in radicular cysts (RCs), dentigerous cysts (DtCs), and keratocystic odontogenic tumors (KCOTs).


METHODS
Forty-nine odontogenic cystic lesions (OCLs) (RCs, n = 20; DtCs, n = 15; KCOTs, n = 14) were assessed using the following markers: S100, CD1a and CD207 for imDCs; and CD83 for mDCs.


RESULTS
Almost all cases were S100, CD1a, and CD207 positive, whereas 63% were CD83 positive. RCs presented greater number of immunostained cells, followed by DtCs, and KCOTs. The number of S100+ cells was greater than both CD1a+ and CD207+ cells (P < 0.001), which showed approximately similar amounts, followed by lower number of CD83+ cells (P < 0.001) in each OCL type. Different from S100+ cells, both CD1a+ and CD207+ cells on the epithelium (P < 0.05) and CD83+ cells on the capsule (P < 0.05) were preferentially observed. In RCs, significant correlation was found between the thickness epithelium with S100+ and CD1a+ cells, and between the degree of inflammation with CD83+ cells.


CONCLUSIONS
Dendritic cell populations in OCLs can be phenotypically heterogeneous, and it could represent distinct lineages and/or functional stages. It is suggested that besides DC-mediated immune cell interactions, DC-mediated tissue differentiation and maintenance in OCLs should also be considered.
Dentistry School, University of Ribeirão Preto (UNAERP), Ribeirão Preto, São Paulo, Brazil.
F T C
FT
Matos
V H T
VH
Rizo
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LY
Almeida
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C
Tirapelli
Y T C
YT
Silva-Sousa
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Almeida
J E
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León
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D018949:Antigens, CD1 / Q000032:analysis; D019070:Cell Lineage; D003713:Dendritic Cells / Q000145:classification* / Q000473:pathology; D003803:Dentigerous Cyst / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D016130:Immunophenotyping; D037181:Lectins, C-Type / Q000032:analysis; D008297:Male; D037241:Mannose-Binding Lectins / Q000032:analysis; D008562:Membrane Glycoproteins / Q000032:analysis; D008875:Middle Aged; D009807:Odontogenic Cysts / Q000473:pathology*; D009808:Odontogenic Tumors / Q000473:pathology; D011842:Radicular Cyst / Q000473:pathology; D009418:S100 Proteins / Q000032:analysis; D000097802:CD83 Antigen
D015703:Antigens, CD; D018949:Antigens, CD1; D007136:Immunoglobulins; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D008562:Membrane Glycoproteins; D009418:S100 Proteins; D000097802:CD83 Antigen; C419393:CD1a antigen; C121916:CD207 protein, human