Gold nanoparticle based dot-blot immunoassay for sensitively detecting Alzheimer's disease related β-amyloid peptide.
A simple, sensitive gold nanoparticle (GNP)-based dot-blot immunoassay has been developed for detecting Alzheimer's disease related β-amyloid peptide 1-42 (Aβ(1-42)) down to a 50 pg mL(-1) level in aqueous solution. Practical samples (cerebrospinal fluid (CSF), cell culturing mediums and cell lysates) have been used to demonstrate the ability of the assay.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China 130022.
Chengke
C
Wang
Dianjun
D
Liu
Zhenxin
Z
Wang
D000544:Alzheimer Disease / Q000175:diagnosis*; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D002460:Cell Line; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D012680:Sensitivity and Specificity
D016229:Amyloid beta-Peptides; D006046:Gold