Compatibility of ascorbate-glutathione cycle enzymes in cyanobacteria against low and high UV-B exposures, simultaneously exposed to low and high doses of chlorpyrifos.
This study deals with the comparative responses of the two cyanobacteria viz. Nostoc muscorum and Phormidium foveolarum against single and combined doses of low (UV-B(L,) 0.1 μmol m(-2) s(-1)) and high (UV-B(H), 1.0 μmol m(-2) s(-1)) fluence rates of ultraviolet-B radiation with low (CP(L), 1 μg ml(-1)) and high (CP(H), 2 μg ml(-1)) doses of the insecticide chlorpyrifos by measuring changes in growth, ascorbate-glutathione cycle enzymes and related metabolites. CP(L) and UV-B(L) both caused lesser increase in ROS but significantly stimulated AsA-GSH cycle enzymes. On the other hand, CP(H) and UV-B(H) posed inhibitory effects by enhancing ROS and inhibiting AsA-GSH cycle enzymes. Inhibitions in CP(H) or UV-B(H) treated samples were significantly prevented when they were supplemented with UV-B(L) and CP(L) (after 72 h), respectively by lowering down ROS and enhancing AsA-GSH enzymes and related metabolites which manifested in terms of improved biomass accumulation.
Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad (A Central University of India), Allahabad, India. prabhatsrivastava.au@gmail.com
Prabhat Kumar
PK
Srivastava
Vijay Pratap
VP
Singh
Sheo Mohan
SM
Prasad
D004390:Chlorpyrifos / Q000633:toxicity*; D000458:Cyanobacteria / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects*; D050502:Nostoc muscorum / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays; D014874:Water Pollutants, Chemical / Q000633:toxicity
D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D004390:Chlorpyrifos