Molecular and functional characterization of endophytic fungi from traditional medicinal plants.
This study reports the isolation of 63 endophytic fungal isolates from two traditional medicinal plants, Ocimum sanctum and Sapindus detergens from different locations of Amritsar, India. The functional characterization of the fungi for their ability to produce anti bacterial and anti cancer agent was carried out. Sixteen strains were characterized at molecular level by sequencing the amplified ITSI-5.8-ITSII region of rDNA. The phylogenetic tree resolved the endophytic fungi into different clades. The fungal endophytes belonging to order Pleosporales (Alternaria sp., Phoma sojicola and Exserohilum sp.) were functionally versatile as they produced diverse biomolecules including antibacterial agent active against Mycobacterium smegmatis, as well as cytotoxic activity against different human cancer cell lines of lung, ovary, breast, prostrate, neuroblastoma and colon.
Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
Jyoti
J
Bhagat
Amarjeet
A
Kaur
Madhunika
M
Sharma
A K
AK
Saxena
B S
BS
Chadha
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D020459:Genes, rRNA; D007194:India; D008969:Molecular Sequence Data; D031339:Ocimum / Q000382:microbiology; D010802:Phylogeny; D010946:Plants, Medicinal / Q000382:microbiology*; D012331:RNA, Fungal / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D032127:Sapindus / Q000382:microbiology; D017422:Sequence Analysis, DNA
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012340:RNA, Ribosomal, 5.8S