Constitutive expression of clathrin hub hinders elicitor-induced clathrin-mediated endocytosis and defense gene expression in plant cells.
Endocytosis has been recently implicated in the signaling network associated with the recognition of microbes by plants. In a previous study, we showed that the elicitor cryptogein was able to induce clathrin-mediated endocytosis (CME) in tobacco suspension cells. Herein, we investigate further the induced CME by means of a GFP-tagged clathrin light chain and a CME inhibitor, the hub domain of clathrin heavy chain. Hub constitutive expression does affect neither cell growth nor constitutive endocytosis but abolishes cryptogein-induced CME. Such an inhibition has no impact on early events in the cryptogein signaling pathway but reduces the expression of defense-associated genes.
UMR Agroécologie 1347, AgroSup/INRA/Université de Bourgogne, Pôle Interaction Plantes Microorganismes, ERL6300 CNRS, Dijon, France.
T
T
Adam
K
K
Bouhidel
C
C
Der
F
F
Robert
A
A
Najid
F
F
Simon-Plas
N
N
Leborgne-Castel
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002966:Clathrin / Q000235:genetics* / Q000378:metabolism*; D033922:Clathrin Heavy Chains / Q000235:genetics / Q000378:metabolism; D033941:Clathrin Light Chains / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis / Q000187:drug effects; D005656:Fungal Proteins / Q000494:pharmacology; D015870:Gene Expression; D017343:Genes, Plant; D046529:Microscopy, Electron, Transmission; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism
D002966:Clathrin; D033941:Clathrin Light Chains; D005656:Fungal Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; C061032:cryptogein protein, Phytophthora cryptogea; D033922:Clathrin Heavy Chains