Single-cell electroporation using a multifunctional pipette.
We present here a novel platform combination, using a multifunctional pipette to individually electroporate single-cells and to locally deliver an analyte, while in their culture environment. We demonstrate a method to fabricate low-resistance metallic electrodes into a PDMS pipette, followed by characterization of its effectiveness, benefits and limits in comparison with an external carbon microelectrode.
Department of Chemical and Biological Engineering, Chalmers University of Technology, S-41296 Göteborg, Sweden.
Alar
A
Ainla
Shijun
S
Xu
Nicolas
N
Sanchez
Gavin D M
GD
Jeffries
Aldo
A
Jesorka
D000818:Animals; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D004129:Dimethylpolysiloxanes; D004566:Electrodes; D018274:Electroporation / Q000295:instrumentation*; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D051381:Rats; D059010:Single-Cell Analysis / Q000295:instrumentation*
D004129:Dimethylpolysiloxanes; C013830:baysilon