[Isolation and identification of Metarhizium from Citrus grandis 'tomentosa' GAP base].
A type of entomopathogenic fungus of soil in Citrus grandis 'tomentosa' production base was isolated and identified with morphological and molecular biological methods, including pathogenesis, spore characteristic and ITS sequence analysis were conducted. The results showed that eighteen entomopathogenic fungi strains were isolated from the Tenebrio molitor infected in the soil samples, which were identified as Metarhizium anisopliae var. anisopliae. Based on results above, we concluded that there was quantity of Metarhizium resources in this area. These provided the useful information for controlling some pests of C. grandis by using these strains of fungus.
The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Weisi
W
Ma
Jiang
J
Xu
Haili
H
Qiao
Jun
J
Chen
Xiangming
X
Li
Rongmin
R
Qin
Huizhen
H
Cheng
D000818:Animals; D002957:Citrus / Q000469:parasitology; D052981:Metarhizium / Q000302:isolation & purification* / Q000502:physiology; D010572:Pest Control, Biological / Q000379:methods*; D012988:Soil Microbiology