Batch production of Pyranose 2-oxidase from Trametes versicolor (ATCC 11235) in medium with a lignocellulosic substrate and enzymatic bleaching of cotton fabrics.
The aim of this work was to determine new, different and low-cost substrates that can be used for enzyme production from the white rot fungus Trametes versicolor (ATCC 11235) by taking advantage of the broad substrate specificity of pyranose 2-oxidase. In this report, we investigated the production of pyranose 2-oxidase from T. versicolor (ATCC 11235) using ten different agricultural residues such as clover straw, almond shells, hazelnut cobs, grass and others. Pyranose 2-oxidase activity was determined as 2.332 U/g at the 9th day in a submerged culture containing clover straw and tap water shaken at 150 rpm and 26°C, and the optimum clover straw concentration was determined to be 12 g/l. The effects of different glucose, nitrogen and phosphate sources on the production of pyranose 2-oxidase were studied in the clover straw medium. Analyses of biomass, protein, reduced sugar and nitrogen concentrations were also monitored in a clover straw medium that did not contain carbon or nitrogen and phosphate sources under the parameters determined. The produced pyranose 2-oxidase was used for improving the properties of cotton fabrics.
Faculty of Science, Biochemistry Department, Ege University, 35100, Bornova, Izmir, Turkey. nurdan.pazarlioglu@ege.edu.tr
Nurdan Kasikara
NK
Pazarlioglu
Emre
E
Erden
M Cigdem
MC
Ucar
Alper
A
Akkaya
A Merih
AM
Sariisik
D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D003368:Gossypium / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D055454:Trametes / Q000201:enzymology* / Q000254:growth & development
D003470:Culture Media; D010710:Phosphates; C036909:lignocellulose; D008031:Lignin; D002237:Carbohydrate Dehydrogenases; C022489:pyranose oxidase; D005947:Glucose; D009584:Nitrogen