Chemoenzymatic deacylation of ramoplanin.
The chemoenzymatic deacylation of ramoplanin A2 is described for the first time: ramoplanin A2 was Boc-protected and hydrogenated to Boc-protected tetrahydroramoplanin, which was subsequently deacylated using an acylase from Actinoplanes utahensis NRRL 12052. The chemoenzymatic process proceeded with 80% overall yield, which favourably compares with the previously described chemical deacylation.
Dipartimento di Scienze Farmaceutiche, Via Venezian 21, 20133 Milano, Italy.
Raffaella
R
Gandolfi
Flavia
F
Marinelli
Enzio
E
Ragg
Diego
D
Romano
Francesco
F
Molinari
D000581:Amidohydrolases / Q000378:metabolism*; D001711:Biotransformation; D047630:Depsipeptides / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008848:Micromonosporaceae / Q000201:enzymology
D047630:Depsipeptides; D006023:Glycoproteins; D000581:Amidohydrolases; C019665:amidase; C416073:ramoplanin A2