Structural and functional development of small intestine in intrauterine growth retarded porcine offspring born to gilts fed diets with differing protein ratios throughout pregnancy.
Protein level in the maternal diet plays a crucial role in fetal programming during pregnancy. Low or high protein level increases the risk of intrauterine growth retardation (IUGR). The aim of this study was to investigate the structural and functional development of the small intestine in piglets from sows fed a control (C, 12.1% protein), a high protein (HP, 30% protein), or a low protein (LP, 6.5% protein) diet during pregnancy. Newborns were classified as IUGR (birth weight ≤1.18 kg) and non-IUGR (birth weight >1.18 kg). The piglets were euthanized on postnatal day (PD)1, PD28 and PD188. The LP diet in non-IUGR neonates resulted in decreased body weight on PD1. The LP and HP diets resulted in both decreased body weight and delayed catch-up growth in the IUGR piglets. The HP and LP-diets increased the length of villi on PD1 in non-IUGRs but not in IUGRs. At birth, the expressions of Ki67 and active caspase 3 in mid-jejunum epithelium of HP and LP non-IUGR neonates were significantly lower as compared to C non-IUGRs whilst in IUGRs the respective expressions were as high as in C non-IUGRs. The postnatal dynamics of brush border enzyme activities and vacuolated enterocytes disappearance showed significant drop in enterocyte maturation in IUGR as compared to non-IUGR neonates. In conclusion, both HP and LP diets led to retarded development of non-IUGR piglets. In IUGR piglets both HP and LP diets resulted in delayed catch-up growth, without adaptive changes in brush border digestive enzymes.
UR 1341, Alimentation Adaptations Digestives, Nerveuses et Comportementales (ADNC), Institut National de la Recherche Agronomique (INRA), Saint Gilles, France.
M
M
Mickiewicz
R
R
Zabielski
B
B
Grenier
L
L
Le Normand
G
G
Savary
J J
JJ
Holst
I P
IP
Oswald
C C
CC
Metges
P
P
Guilloteau
D000824:Animal Nutritional Physiological Phenomena / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D001724:Birth Weight / Q000235:genetics / Q000502:physiology; D001835:Body Weight / Q000235:genetics / Q000502:physiology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002766:Cholecystokinin / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004032:Diet; D004044:Dietary Proteins / Q000378:metabolism*; D005260:Female; D047109:Fetal Development / Q000235:genetics / Q000502:physiology; D005317:Fetal Growth Retardation / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D004763:Glucagon-Like Peptides / Q000235:genetics / Q000378:metabolism; D007249:Inflammation / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism / Q000502:physiology; D007583:Jejunum / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008297:Male; D008938:Mitosis / Q000235:genetics / Q000502:physiology; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D034421:Sus scrofa / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
D016207:Cytokines; D004044:Dietary Proteins; D019394:Ki-67 Antigen; D012333:RNA, Messenger; D004763:Glucagon-Like Peptides; D002766:Cholecystokinin; D053148:Caspase 3