Novel molecular beacons to monitor microRNAs in non-small-cell lung cancer.
Lung cancer is the leading cause of cancer death worldwide. There is no effective early diagnostic technology for lung cancer. microRNAs (miRNAs) are noncoding RNA molecules which regulate the process of cell growth and differentiation in human cancers. Hsa-miR-155 (miR-155), highly expressed in non-small-cell lung cancer (NSCLC), can be used as a diagnostic marker for NSCLC. Dynamic observation of miR-155 is critical to diagnose NSCLC. A novel molecular beacon (MB) of miR-155 was designed to image the expression of miR-155 in NSCLC. Then miR-155 was detected in vitro by laser confocal microscopy and in vivo by stereomicroscope imaging system, respectively. The present study demonstrated that intracellular miR-155 could be successfully and quickly detected by novel miR-155 MBs. As a noninvasive monitoring approach, MBs could be used to diagnose lung cancer at early stage through molecular imaging.
Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
Quan
Q
Yao
An-mei
AM
Zhang
Hu
H
Ma
Sheng
S
Lin
Xin-xin
XX
Wang
Jian-guo
JG
Sun
Zheng-tang
ZT
Chen
D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis* / Q000235:genetics*; D017931:DNA Primers; D031541:Fluorescence Resonance Energy Transfer; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D018613:Microscopy, Confocal; D015335:Molecular Probes / Q000235:genetics*; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D023041:Xenograft Model Antitumor Assays
D014408:Biomarkers, Tumor; D017931:DNA Primers; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D015335:Molecular Probes