Adenovirus serotype 26 utilizes CD46 as a primary cellular receptor and only transiently activates T lymphocytes following vaccination of rhesus monkeys.
The cellular receptor utilized by adenovirus serotype 26 (Ad26) has remained unclear. Here we show that Ad26 transduction is CD46-dependent and is efficiently blocked by anti-CD46 but not anti-CAR antibodies, demonstrating that Ad26 utilizes CD46 as a primary cellular receptor. Moreover, following Ad26 vaccination of rhesus monkeys, we did not observe sustained activation of peripheral or mucosal vector-specific CD4(+) T lymphocytes. These data contribute to our understanding of Ad26 as a candidate vaccine vector.
Division of Vaccine Research, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
Hualin
H
Li
Elizabeth G
EG
Rhee
Katherine
K
Masek-Hammerman
Jeffrey E
JE
Teigler
Peter
P
Abbink
Dan H
DH
Barouch
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism*; D000257:Adenoviridae Infections / Q000276:immunology / Q000821:virology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein / Q000737:chemistry* / Q000378:metabolism; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007249:Inflammation; D007963:Leukocytes, Mononuclear / Q000821:virology; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D051916:Membrane Cofactor Protein / Q000737:chemistry*; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology*; D014611:Vaccination
D000911:Antibodies, Monoclonal; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D051916:Membrane Cofactor Protein; D049452:Green Fluorescent Proteins