The use of biotin to demonstrate immunohistochemistry, Western blotting, and dot blots in university practical classes.
Immunological detection of proteins is an essential method to demonstrate to undergraduate biology students, however, is often difficult in resource and time poor student laboratory sessions. This method describes a failsafe method to rapidly and economically demonstrate this technique using biotinylated proteins or biotin itself as targets for detection. This negates the need for a specific primary antibody, saving cost and time. In addition, the easily available and safe reagents allow the methods to be readily adopted without specialist technical expertise. As a result, staff can confidently transfer ownership of the task to the student so as to also develop scientific inquiry skills which promotes student motivation and engagement.
University of Western Sydney, School of Science and Health, Penrith, NSW 2751, Australia.
Thomas James
TJ
Millar
Ronald
R
Knighton
Jo-Anne
JA
Chuck
D001695:Biology / Q000193:education*; D001710:Biotin; D015153:Blotting, Western; D006801:Humans; D007150:Immunohistochemistry; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D012106:Research / Q000193:education*; D013663:Teaching / Q000379:methods; D014495:Universities
D011506:Proteins; D001710:Biotin