Scale-up of an alkaline protease from Bacillus pumilus MTCC 7514 utilizing fish meal as a sole source of nutrients.
Fish meal grades SL1 and SL2 from Sardine (Sardinella longiceps) and NJ from Pink Perch (Nemipterus japonicas) were evaluated as a sole source of carbon and nitrogen in the medium for alkaline protease production by Bacillus pumilus MTCC 7514. The analysis of the fish meal suggests that the carbon and nitrogen contents in fish meal are sufficient to justify its choice as replacement for other nutrients. Protease production increased significantly (4,914 U/ml) in medium containing only fish meal, compared with the basal medium (2,646 U/ml). However, the elimination of inorganic salts from media reduced the protease productivity. In addition, all the three grades of fish meal yielded almost the same amounts of protease when employed as the sole source of carbon and nitrogen. Nevertheless, the best results were observed in fish meal SL1 medium. Furthermore, protease production was enhanced to 6,966 U/ml and 7,047 U/ml on scaling up from flask (4,914 U/ml) to 3.7 and 20 L fermenters, respectively, using fish meal (10 g/l). Similarly, the corresponding improvement in productivities over flask (102.38 U/ml/h) was 193.5 and 195.75 U/ml/h in 3.7 and 20 L fermenters, respectively. The crude protease was found to have dehairing ability in leather processing, which is bound to have great environmental benefits.
Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai, India.
Rishikesh Kumar
RK
Gupta
Dinesh
D
Prasad
Jaykumar
J
Sathesh
Ramachandra Boopathy
RB
Naidu
Numbi Ramudu
NR
Kamini
Saravanan
S
Palanivel
Marichetti Kuppuswami
MK
Gowthaman
D000818:Animals; D001407:Bacillus / Q000201:enzymology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D010450:Endopeptidases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D005396:Fish Products; D006041:Goats; D006197:Hair / Q000378:metabolism; D007218:Industrial Microbiology; D008892:Milk; D011134:Polysaccharides; D013633:Tanning
D001426:Bacterial Proteins; D003470:Culture Media; D011134:Polysaccharides; C008315:maltodextrin; D010450:Endopeptidases; C087822:alkaline protease