The NS1 protein of influenza A virus blocks RIG-I-mediated activation of the noncanonical NF-κB pathway and p52/RelB-dependent gene expression in lung epithelial cells.
Influenza A virus (IAV) infection of epithelial cells activates NF-κB transcription factors via the canonical NF-κB signaling pathway, which modulates both the antiviral immune response and viral replication. Since almost nothing is known so far about a function of noncanonical NF-κB signaling after IAV infection, we tested infected cells for activation of p52 and RelB. We show that the viral NS1 protein strongly inhibits RIG-I-mediated noncanonical NF-κB activation and expression of the noncanonical target gene CCL19.
Institute of Molecular Virology (IMV), Centre for Molecular Biology of Inflammation (ZMBE), Westfälische-Wilhelms-University, Muenster, Germany.
Andrea
A
Rückle
Emanuel
E
Haasbach
Ilkka
I
Julkunen
Oliver
O
Planz
Christina
C
Ehrhardt
Stephan
S
Ludwig
D002460:Cell Line; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology; D015870:Gene Expression; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology / Q000502:physiology; D006801:Humans; D009980:Influenza A virus / Q000472:pathogenicity / Q000502:physiology*; D008168:Lung / Q000378:metabolism / Q000821:virology; D016328:NF-kappa B / Q000378:metabolism*; D052003:NF-kappa B p52 Subunit / Q000378:metabolism; D011971:Receptors, Immunologic; D015398:Signal Transduction; D051841:Transcription Factor RelB / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000502:physiology*
C085820:INS1 protein, influenza virus; D016328:NF-kappa B; D052003:NF-kappa B p52 Subunit; C497593:RELB protein, human; D011971:Receptors, Immunologic; D017361:Viral Nonstructural Proteins; D051841:Transcription Factor RelB; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases