Fluorescence detection of adenosine triphosphate in an aqueous solution using a combination of copper(II) complexes.
Fluorescent ligands have been designed to form ternary complexes with a Cu(II) cation and phosphates in a buffer solution at physiological pH 7.4. It has been shown that a combination of two different ligands and CuCl(2) allows one to achieve high adenosine triphosphate/adenosine diphosphate, adenosine 5'-monophosphate selectivity, and ratiometric fluorescence sensing, while separately each ligand complex does not have such properties.
Fakultät für Naturwissenschaften, Institut für Chemie, Technische Universität Chemnitz, Straße der Nationen 62, 09111 Chemnitz, Germany. evgeny.kataev@chemie.tu-chemnitz.de
Evgeny
E
Kataev
René
R
Arnold
Tobias
T
Rüffer
Heinrich
H
Lang
D000244:Adenosine Diphosphate / Q000032:analysis*; D000249:Adenosine Monophosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis*; D000873:Anthracenes / Q000737:chemistry*; D002021:Buffers; D056831:Coordination Complexes / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D011199:Potentiometry; D011743:Pyrimidines / Q000737:chemistry*; D011789:Quantum Theory; D012680:Sensitivity and Specificity; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014867:Water
D000873:Anthracenes; D002021:Buffers; D056831:Coordination Complexes; D008024:Ligands; D011743:Pyrimidines; D012996:Solutions; C495751:aminomethylpyrimidine; D014867:Water; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D003300:Copper; D000255:Adenosine Triphosphate; C034020:anthracene