Temperate membrane-containing halophilic archaeal virus SNJ1 has a circular dsDNA genome identical to that of plasmid pHH205.
A temperate haloarchaeal virus, SNJ1, was induced from the lysogenic host, Natrinema sp. J7-1, with mitomycin C, and the virus produced plaques on lawns of Natrinema sp. J7-2. Optimization of the induction conditions allowed us to increase the titer from ~10(4) PFU/ml to ~10(11) PFU/ml. Single-step growth curves exhibited a burst size of ~100 PFU/cell. The genome of SNJ1 was observed to be a circular, double-stranded DNA (dsDNA) molecule (16,341 bp). Surprisingly, the sequence of SNJ1 was identical to that of a previously described plasmid, pHH205, indicating that this plasmid is the provirus of SNJ1. Several structural protein-encoding genes were identified in the viral genome. In addition, the comparison of putative packaging ATPase sequences from bacterial, archaeal and eukaryotic viruses, as well as the presence of lipid constituents from the host phospholipid pool, strongly suggest that SNJ1 belongs to the PRD1-type lineage of dsDNA viruses, which have an internal membrane.
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Ziqian
Z
Zhang
Ying
Y
Liu
Shuai
S
Wang
Di
D
Yang
Yichen
Y
Cheng
Jiani
J
Hu
Jin
J
Chen
Yunjun
Y
Mei
Ping
P
Shen
Dennis H
DH
Bamford
Xiangdong
X
Chen
D000595:Amino Acid Sequence; D023641:Archaeal Viruses / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D023061:Gene Order; D016679:Genome, Viral; D006216:Halobacteriaceae / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D011533:Proviruses / Q000235:genetics*; D017422:Sequence Analysis, DNA; D019562:Viral Load; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics; D014775:Virus Activation
D004270:DNA, Circular; D004279:DNA, Viral; D014764:Viral Proteins; D004247:DNA