Protocol for a facile multiplex PCR for multi-antimicrobial resistance and Gonococcus detection.
Gonorrhea is a continuing problem worldwide particularly in terms of the spread of multiple drug resistance. We have successfully developed an efficient PCR method for the simultaneous identification of gonococci and detection of the antimicrobial-resistant profile. By this method, penicillinase-producing Neisseria gonorrhoeae (PPNG), high-level tetracycline-resistant N. gonorrhoeae (TRNG), and ciprofloxacin-resistant N. gonorrhoeae (CRNG) can be clearly identified. Moreover, the plasmid-types of penicillin and tetracycline resistance are also characterized. The method has 100 % sensitivity and specificity. It is also time- and labor-saving compared to the conventional method. Thus, the procedure is suitable for epidemiological surveillance.
Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
Ratana
R
Lawung
Rungrot
R
Cherdtrakulkiat
Virapong
V
Prachayasittikul
D000890:Anti-Infective Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics*; D006069:Gonorrhea / Q000175:diagnosis / Q000517:prevention & control; D006801:Humans; D017720:Molecular Epidemiology; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D012150:Polymorphism, Restriction Fragment Length
D000890:Anti-Infective Agents; D004269:DNA, Bacterial