Effect of moderate ethanol administration on biochemical indices in streptozotocin-diabetic Wistar rats.
OBJECTIVE
This study was designed to evaluate the effect of moderate ethanol administration on the biochemical indices in streptozotocin (STZ)-diabetic rats.


METHODS
Twenty-four male Wistar rats were divided into four groups of six animals each. Groups one and two contained non-diabetic normal rats and normal rats treated with ethanol, respectively. Group three was untreated STZ-diabetic rats and group four was made up of ethanol-treated STZ-diabetic rats. Diabetes was induced by a single intraperitoneal injection of STZ (35 mg/kg), while ethanol (100%v/v) was given at a dose 2 g/kg thrice per week for three weeks. After the last dose of ethanol and an overnight fasting, rats were sacrificed by cervical dislocation. Blood was collected by syringe from the heart into plain centrifuge tubes.


RESULTS
Moderate ethanol administration to STZ-diabetic rats caused a significant (p < 0.05) increase in relative weight of liver relative to normal. Ethanol intake in STZ-diabetic rats produced an insignificant (p > 0.05) effect on the levels of fasting blood glucose (FBG) and HbA1c relative to the untreated-diabetic group. Moderately, ethanol administration to STZ-diabetic rats produced a marked and significant (p < 0.05) increase in the levels of serum total cholesterol, triglycerides, low-density lipoprotein (LDL)-cholesterol and the activities of alanine aminotransferase relative to untreated diabetic rats. Ethanol-treated diabetic rats had significantly (p < 0.05) lower high-density lipoprotein (HDL)-cholesterol levels, while the activities of lactate dehydrogenase and alpha-amylase were insignificantly (p > 0.05) affected. There were no significant (p > 0.05) differences in all the biochemical indices in normal rats relative to ethanol-treated normal rats.


CONCLUSIONS
Moderate ethanol administration did not affect FBG and HbA1c, but altered the lipid profile of STZ-diabetic rats. Moderate ethanol intake may further increase the risk of complications in diabetes.
Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, University of Ibadan, Nigeria. aoadaramoye@yahoo.com
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D000410:Alanine Transaminase / Q000097:blood / Q000187:drug effects; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects*; D002492:Central Nervous System Depressants / Q000008:administration & dosage* / Q000494:pharmacology; D008076:Cholesterol, HDL / Q000097:blood / Q000187:drug effects; D008078:Cholesterol, LDL / Q000097:blood / Q000187:drug effects; D003920:Diabetes Mellitus / Q000097:blood* / Q000139:chemically induced; D000431:Ethanol / Q000008:administration & dosage* / Q000494:pharmacology; D006442:Glycated Hemoglobin / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D014280:Triglycerides / Q000097:blood; D000516:alpha-Amylases / Q000097:blood / Q000187:drug effects
D000410:Alanine Transaminase; D001786:Blood Glucose; D002492:Central Nervous System Depressants; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D000431:Ethanol; D006442:Glycated Hemoglobin; D007770:L-Lactate Dehydrogenase; D013311:Streptozocin; D014280:Triglycerides; D000516:alpha-Amylases