Characterization of the newly isolated Geobacillus sp. T1, the efficient cellulase-producer on untreated barley and wheat straws.
A thermophile cellulase-producing bacterium was isolated and identified as closely related to Geobacillus subterraneus. The strain, named Geobacillus sp. T1, was able to grow and produce cellulase on cellobiose, microcrystalline cellulose, carboxymethylcellulose (CMC), barley straw, wheat straw and Whatman No. 1 filter paper. However, barley and wheat straws were significantly better substrates for cellulase production. When Geobacillus sp. T1 was cultivated in the presence of 0.5% barley straw, 0.1% Tween 80 and pH 6.5 at 50°C, the maximum level of free cellulase up to 143.50 U/mL was produced after 24h. This cellulase (≈ 54 kDa) was most active at pH 6.5 and 70°C. The enzyme in citrate phosphate buffer (10mM) was stable at 60°C for at least 1h. Geobacillus sp. T1 with efficient growth and cellulase production on straws seems a potential candidate for conversion of agricultural biomass to fuels.
Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran 14155-6343, Iran.
Reza
R
Assareh
Hossein
H
Shahbani Zahiri
Kambiz
K
Akbari Noghabi
Saeed
S
Aminzadeh
Gholamreza
G
Bakhshi Khaniki
D002244:Carbon / Q000494:pharmacology; D002480:Cellulase / Q000096:biosynthesis* / Q000302:isolation & purification; D002482:Cellulose / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004275:DNA, Ribosomal / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability / Q000187:drug effects; D056506:Geobacillus / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification* / Q000648:ultrastructure; D001467:Hordeum / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D011136:Polysorbates / Q000494:pharmacology; D012988:Soil Microbiology; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D014908:Triticum / Q000737:chemistry*; D014866:Waste Products / Q000032:analysis*
D003470:Culture Media; D004275:DNA, Ribosomal; D011136:Polysorbates; D014866:Waste Products; D002244:Carbon; D002482:Cellulose; D002480:Cellulase