New rapid screening method for anti-aging compounds using budding yeast and identification of beauveriolide I as a potent active compound.
The chronological lifespan (CLS) of budding yeast is a model for the aging of post-mitotic cells in higher eukaryotes. We report here the development of a new method to assess yeast CLS. The new assay is simple, convenient and labor-saving. We applied this new method to screen natural compounds isolated from mushrooms and discovered beauveriolide I as a potent anti-aging agent.
Faculty of Science, Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan.
Shigeru
S
Nakaya
Saki
S
Mizuno
Hiroki
H
Ishigami
Yasuhiro
Y
Yamakawa
Hirokazu
H
Kawagishi
Takashi
T
Ushimaru
D000363:Agaricales / Q000737:chemistry*; D016922:Cellular Senescence / Q000187:drug effects* / Q000502:physiology; D003575:Cytochromes c1 / Q000172:deficiency / Q000235:genetics; D047630:Depsipeptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015870:Gene Expression; D057166:High-Throughput Screening Assays; D056466:Histone Demethylases / Q000172:deficiency / Q000235:genetics; D006801:Humans; D008017:Life Expectancy; D008136:Longevity; D008938:Mitosis / Q000187:drug effects; D008954:Models, Biological; D019869:Phosphatidylinositol 3-Kinases / Q000172:deficiency / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
D003575:Cytochromes c1; D047630:Depsipeptides; D056466:Histone Demethylases; D019869:Phosphatidylinositol 3-Kinases; D029701:Saccharomyces cerevisiae Proteins; C013833:beauverolides; C400297:GIS1 protein, S cerevisiae; C083324:TOR1 protein, S cerevisiae