Polymer adhesive surface as flexible generic platform for multiplexed assays biochip production.
The present report describes the integration and application possibilities of a new microarray concept based on adhesive surface. The method was shown to enable the straightforward production of 384 and 1536-well plates modified with 100 and 25 spots per well, respectively. Such in-well densities were only possible thanks to the fabrication process which implies first the deposition of the microarray on a flat adhesive surface and then its assembly with bottomless 384 or 1536-well plates. The concept was also confronted to various applications such as oligonucleotide detection, localised cell culture onto spotted adhesion proteins and immobilisation of peptide or active antibodies for immunoassays. In the particular case of immunotesting, the study focused on liver diseases diagnosis and more particularly on the detection of either one liver cancer marker, the alpha-fetoprotein, or the detection of Hepatitis C Virus infection. In every cases, interesting performances were obtained directly in crude patient serum, proof of the robust and generic aspect of the platform.
Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Claude Bernard Lyon 1-University of Lyon-CNRS 5246 ICBMS, Bât. CPE-43 Bd du 11 Nov.1918, 69622 Villeurbanne, France.
Céline A
CA
Mandon
Ophélie I
OI
Berthuy
Benjamin P
BP
Corgier
Gaelle C
GC
Le Goff
Patrice
P
Faure
Patrice N
PN
Marche
Loïc J
LJ
Blum
Christophe A
CA
Marquette
D000269:Adhesives / Q000737:chemistry*; D055910:Antibodies, Immobilized / Q000032:analysis; D004867:Equipment Design; D006367:HeLa Cells; D006526:Hepatitis C / Q000175:diagnosis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D008113:Liver Neoplasms / Q000175:diagnosis; D046228:Microarray Analysis / Q000295:instrumentation*; D009841:Oligonucleotides / Q000032:analysis; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000032:analysis
D000269:Adhesives; D055910:Antibodies, Immobilized; D009841:Oligonucleotides; D011108:Polymers; D000509:alpha-Fetoproteins