[Screening of pancreatic lipase and alpha-glucosidase inhibitors from Chinese dietary herbs].
The present study was conducted to develop new inhibitors of pancreatic lipase and alpha-glucosidase from Chinese dietary herbs. Sixty-three dietary herbs from 39 taxonomic families were selected and extracted with aqueous ethanol or water. The extracts were then tested with in vitro enzyme assays for their ability to inhibit pancreatic lipase and alpha-glucosidase activities. Orlistat and acarbose were used as two positive controls. The extracts of Nelumbo nucifera, Curcuma longa, Piper longum and Morus alba showed strong pancreatic lipase inhibitory effects with IC50 at (28.00 +/- 5.51), (5.24 +/- 0.51), (14.76 +/- 2.58), (4.78 +/- 0.58), (3.41 +/- 0.67) mg x L(-1), respectively. These extracts also showed potent alpha-glucosidase inhibitory activities with IC50 at (1.98 +/- 0.13), (0. 18 + 0.007), (0.71 +/- 0.08), (0.077 +/- 0.005), (0.089 +/- 0.006) g x L(-1), respectively. The results provide useful information for developing new drugs or natural health products for hyperlipidemia and hypoglycemia from Chinese dietary herbs.
School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
Xiaoli
X
Sun
Kaibin
K
Zhang
Xiuhong
X
Ji
Yanwen
Y
Wang
Zidichouski
Z
Jeffrey
Yan
Y
Tong
Huimin
H
Gao
Junzeng
J
Zhang
Zhimin
Z
Wang
D030024:Curcuma / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D008049:Lipase / Q000037:antagonists & inhibitors*; D031625:Morus / Q000737:chemistry; D031653:Nelumbo / Q000737:chemistry; D010179:Pancreas / Q000201:enzymology*; D031701:Piper / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D000520:alpha-Glucosidases / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D008049:Lipase; D000520:alpha-Glucosidases