Assessment of  genetic diversity of landraces of Dendrocalamus hamiltonii using AFLP  markers and association with biochemical traits.
Fermented  bamboo shoots are popular traditional food items of various ethnic groups of  the northeastern India, especially in Manipur State. Dendrocalamus  hamiltonii is an economically important bamboo species used to produce  fermented bamboo shoots. We studied genetic variability of this bamboo species  in Chandel and Imphal-East (commercial production districts), using AFLP  molecular markers. Each of the selected primers detected polymorphisms and 1614  (95.8%) were found to be polymorphic. Cluster analysis based on Dice similarity  coefficients using UPGMA differentiated the populations into two major groups.  Principal coordinate analysis based on the AFLP data clearly separated the  populations according to their genetic diversity and antioxidant activity. Four  primers were tested through multiple regression analysis to identify  marker-trait association between AFLP data and biochemical attributes, i.e.,  antioxidant activity and total cyanide content. The 273 bp generated by EcoRI-AAG(Joe)/MseI-CTC  showed high positive correlation with antioxidant activity (r = 0.729, P <  0.01). The 396 bp generated by EcoRI-AAC(Ned)/MseI-CTG were  negatively correlated with cyanide content (r = -0.694, P < 0.01). Thus, we  found association of DNA markers with antioxidant activities and total cyanide content.  These results could be of use for the identification of superior genotypes with  desirable traits.
Institute of Bioresource and Sustainable Development, Imphal, India.
S D
SD
Waikhom
S
S
Ghosh
N C
NC
Talukdar
S S
SS
Mandi
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D000975:Antioxidants / Q000494:pharmacology; D031723:Bambusa / Q000033:anatomy & histology / Q000187:drug effects / Q000235:genetics*; D001713:Biphenyl Compounds / Q000378:metabolism; D003486:Cyanides / Q000378:metabolism; D060146:Ecotype; D016166:Free Radical Scavengers / Q000494:pharmacology; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D005843:Geography; D007194:India; D010802:Phylogeny; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D011110:Polymorphism, Genetic; D025341:Principal Component Analysis; D019655:Quantitative Trait, Heritable; D012044:Regression Analysis
D000975:Antioxidants; D001713:Biphenyl Compounds; D003486:Cyanides; D016166:Free Radical Scavengers; D005819:Genetic Markers; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl