Effect of exogenous erythritol on growth and survival of Brucella.
Erythritol has been considered as an important factor for the pathogenesis of Brucella abortus 2308 and its ability to cause abortion in ruminants. There is a lack of laboratory models to study the Brucella-erythritol relationship, as commonly used murine models do not have erythritol. We tested the effect of exogenous erythritol on the growth of Brucella in iron minimal medium (IMM), in infected macrophage culture and in infected mice to determine if these models can be used to study the relationship between Brucella and erythritol. An effect of erythritol on Brucella growth was only seen in IMM. There appear to be no effect of erythritol on Brucella growth in macrophage cell cultures or in mice. This shows that administration of erythritol to the mice or macrophages cannot mimic the environment in ruminants during pregnancy and thus cannot be used as models to understand the effect of erythritol on Brucella pathogenesis.
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24061, USA.
Neeta
N
Jain
Stephen M
SM
Boyle
Nammalwar
N
Sriranganathan
D000818:Animals; D002003:Brucella abortus / Q000187:drug effects* / Q000254:growth & development; D002006:Brucellosis / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D004195:Disease Models, Animal; D004896:Erythritol / Q000494:pharmacology*; D007501:Iron / Q000494:pharmacology; D007928:Lethal Dose 50; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
D003470:Culture Media; D014665:Vasodilator Agents; D007501:Iron; D004896:Erythritol