Silica-based gene reverse transfection: an upright nanosheet network for promoted DNA delivery to cells.
A method for substrate-mediated reverse gene transfection was developed using a silica film composed of an upright-sheet network. The silica film with a dense upright-sheet network shows approximately double higher transgene expression efficiency than that of solution-based transfection.
World Premier International (WPI) Research Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba 305-0044, Japan. JI.Qingmin@nims.go.jp
Qingmin
Q
Ji
Tomohiko
T
Yamazaki
Nobutaka
N
Hanagata
Michael V
MV
Lee
Jonathan P
JP
Hill
Katsuhiko
K
Ariga
D004247:DNA / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D014162:Transfection
D049452:Green Fluorescent Proteins; D012822:Silicon Dioxide; D004247:DNA