Natural fruit extracts as non-toxic fluorescent dyes for staining fungal chlamydospores.
Currently, most synthetic dyes utilized for fungal fluorescent staining are toxic, carcinogenic, or harmful to animals, humans, and the environment. This study proposes non-toxic extracts of fruits from the genera Rhamnus, Ribes, Sambucus, Viburnum, Sorbus and Beta as simple, safe, and ecological alternatives to chemical fluorescent dye for efficient staining of Fusarium chlamydospore cells using, as test strains, five different pathogenic Fusarium species.
Department of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, S7N 5A8 SK, Canada.
Silva
S
Vujanovic
Yit Kheng
YK
Goh
Vladimir
V
Vujanovic
D005456:Fluorescent Dyes / Q000302:isolation & purification*; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D005524:Food Technology; D005638:Fruit / Q000737:chemistry*; D005670:Fusarium / Q000302:isolation & purification / Q000472:pathogenicity*; D008856:Microscopy, Fluorescence; D010936:Plant Extracts / Q000737:chemistry; D013172:Spores, Fungal / Q000302:isolation & purification; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D010936:Plant Extracts