Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro.
The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r=0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach.
Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Sontana
S
Siritantikorn
Suthatip
S
Jintaworn
Sansanee
S
Noisakran
Yupin
Y
Suputtamongkol
Daniel H
DH
Paris
Stuart D
SD
Blacksell
D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D012285:Orientia tsutsugamushi / Q000302:isolation & purification* / Q000378:metabolism; D015203:Reproducibility of Results; D012612:Scrub Typhus / Q000175:diagnosis* / Q000378:metabolism; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D001426:Bacterial Proteins