Isotherm and kinetic models and cell surface analysis for determination of the mechanism of metal sorption by Aspergillus versicolor.
The isolate Aspergillus versicolor was obtained from an estuary, which is exposed to metal contamination. It was found to have a good metal tolerance and sorption capacity. Further studies revealed that the rate of metal removal from solution is very rapid in the first 5-10 min, and is favoured by a pH of 6.0. The biosorption data obtained was explained by the Freundlich adsorption isotherm model and followed a pseudo-second order kinetics reaction. The fungus showed a higher accumulation of fatty acids when grown in presence of metals as compared to the mycelium grown in absence of the metal; there was also an increase in the saturation index of fatty acids in presence of Cu(2+) which serves as a protective mechanism for the fungus. Fourier Transform Infrared, scanning electron microscopy and EDAX analysis indicated that metal removal from solution by A. versicolor occurred by a passive adsorption to the fungal cell surface, involving an ion exchange mechanism.
Department of Microbiology, Goa University, Taleigao Plateau, 403206, Goa, India.
Mufedah A H
MA
Gazem
Sarita
S
Nazareth
D001230:Aspergillus / Q000378:metabolism* / Q000648:ultrastructure; D003300:Copper / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D007700:Kinetics; D007854:Lead / Q000378:metabolism; D008670:Metals / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared
D005227:Fatty Acids; D008670:Metals; D007854:Lead; D003300:Copper