A protocol for measurement of noncoding RNA in human serum.
MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Currently, there are many methods of isolating total RNA from liquid samples. Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels.
O'Brien Institute, 42 Fitzroy Street, Fitzroy, VIC 3065, Australia. tayc@unimelb.edu.au
Caroline J
CJ
Taylor
Sarang N
SN
Satoor
Amaresh K
AK
Ranjan
Maria V
MV
Pereira e Cotta
Mugdha V
MV
Joglekar
D015415:Biomarkers / Q000378:metabolism; D005786:Gene Expression Regulation; D005821:Genetic Techniques; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000097:blood / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D022661:RNA, Untranslated / Q000032:analysis / Q000097:blood* / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D044967:Serum / Q000378:metabolism*
D015415:Biomarkers; D035683:MicroRNAs; D012333:RNA, Messenger; D022661:RNA, Untranslated