In vitro observations of self-assembled ECM-mimetic bioceramic nanoreservoir delivering rFN/CDH to modulate osteogenesis.
The cell-extracellular matrix (ECM) interaction has been employed to direct tissue-specific cellular responses and tailor biomaterial development. Given the important roles of integrin-fibronectin interactions and homophilic cadherin responses, we postulated that the recreation of the biological function of ECM in a recombinant fibronectin/cadherin chimera (rFN/CDH) at the nanoscale may properly address the integration impediment at the interface. This study aimed to investigate the feasibility and validity of an rFN/CDH bio-inspired ceramic surface generated via layer-by-layer (LbL) self-assembly to induce osteogenesis. The successful fabrication of a multilayered structure of BCP/LbL/[Chi-rFN/CDH] and excellent physico-chemical properties were confirmed by X-ray photoelectron spectroscopy, Fourier-transform infrared spectroscopy, contact angle, and scanning electron microscopy. Sustained release was observed during degradation, and the multilayered construct also displayed a significantly enhanced ability to guide bMSC adhesion, proliferation, and osteogenic differentiation. A mechanistic exploration revealed that the interaction of rFN/CDH with integrin α5β1/αvβ3 may be a regulatory pivot. These results demonstrate the hybrid potential of bioceramics coated with biofunctional rFN/CDH multilayers in osteoconductive and osteoinducive potency. This study therefore presents a basis for fabricating potential bio-functionalised ceramic-based substitutions for preclinical and clinical application to bone defects and reconstruction.
Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, PR China.
Yuan
Y
Zhang
Liqi
L
Li
Jie
J
Zhu
Hong
H
Kuang
Shiwu
S
Dong
Hongwei
H
Wang
Xia
X
Zhang
Yue
Y
Zhou
D000818:Animals; D040761:Biomimetic Materials / Q000494:pharmacology*; D015820:Cadherins / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D016503:Drug Delivery Systems / Q000379:methods*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D005353:Fibronectins / Q000494:pharmacology; D020869:Gene Expression Profiling; D005818:Genetic Engineering; D016023:Integrins / Q000378:metabolism; D008024:Ligands; D059630:Mesenchymal Stem Cells / Q000166:cytology; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D056951:Photoelectron Spectroscopy; D021122:Protein Subunits / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties / Q000187:drug effects
D015820:Cadherins; D005353:Fibronectins; D016023:Integrins; D008024:Ligands; D021122:Protein Subunits; D011994:Recombinant Proteins