Phenotype of peripheral blood cells mobilized by granulocyte colony-stimulating factor in patients with chronic heart failure.
Administration of granulocyte CSF preparation to patients with chronic heart failure produced a hemostimulating effect and increased the content of leukocytes and neutrophils in the peripheral blood. Granulocyte CSF induced mobilization of bone marrow progenitor cells into the peripheral blood. The content of hemopoietic CD34(+)progenitor cells, which attained 0.42% (0.25-0.64) by the end of mobilization, inversely correlated with the number of myocardial infarctions. Administration of granulocyte CSF not only led to mobilization of bone marrow hemopoietic cells, but also increased the pool of endothelial progenitor cells in the peripheral blood: the content of CD34(+)/CD133(+)and CD34(+)/KDR(+)attained 0.02% (0.013-0.075) and 0.1% (0.05-0.20), respectively. Peripheral blood is an available source of progenitor cells, while mononuclear cells after administration of granulocyte CSF can produce a reparative effect on ischemic myocardium.
Research Institute of Clinical and Experimental Lymphology, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia.
V I
VI
Konenkov
E A
EA
Pokushalov
O V
OV
Poveshchenko
I I
II
Kim
A B
AB
Romanov
N A
NA
Guleva
V V
VV
Bernvald
A O
AO
Soloviova
E V
EV
Yankayte
A F
AF
Poveshchenko
A M
AM
Karaskov
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006333:Heart Failure / Q000378:metabolism* / Q000628:therapy; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000378:metabolism / Q000628:therapy; D010455:Peptides / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D016179:Granulocyte Colony-Stimulating Factor