Expression of multipotent and retinal markers in pigment epithelium of adult human in vitro.
Immunoperoxidase and molecular genetic analysis showed that retinal pigment epithelial cells from adult human eye undergo morphogenetic changes in vitro. They lose expression of tissue-specific protein RPE65 and start to express stem cell markers: Oct4 (POU5F1), Nanog, Prox1, Musashi 1, and Pax6, which indicates their differentiation. Expression of Musashi 1 and Pax6 attest to neural differentiation, which is also confirmed by the expression of βIII-tubulin, a neuroblast marker, and markers of differentiated neuronal cells, tyrosine hydroxylase and neurofilament proteins. These findings attest to the capacity of retinal pigment epithelium from adult human eye to transdifferentiation into neural lineage cells, which makes them an interesting object for cell therapy in neurodegeneration.
N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. milyushina@rambler.ru
L A
LA
Milyushina
B I
BI
Verdiev
A V
AV
Kuznetsova
M A
MA
Aleksandrova
D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D019745:cis-trans-Isomerases / Q000235:genetics / Q000378:metabolism; D000098551:Prospero-Related Homeobox 1 Protein; D000099178:Retinoid Isomerohydrolase
D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; D019745:cis-trans-Isomerases; D000098551:Prospero-Related Homeobox 1 Protein; D000099178:Retinoid Isomerohydrolase; C486780:NANOG protein, human; C000605730:PAX6 protein, human; C494173:POU5F1 protein, human