Rapid identification of Mycobacterium tuberculosis in BACTEC MGIT960 cultures by in-house loop-medicated isothermal amplification.
Definitive diagnosis of tuberculosis (TB) by conventional culture, followed by bacterial  identification based on biochemical tests is time-consuming and tedious. Simple loop-mediated isothermal  amplification (LAMP) specific for Mycobacterium tuberculosis complex, targeting the M. tuberculosis 16S  ribosomal RNA gene, termed TB-LAMP, was evaluated as an alternative for rapid culture confirmation.  TB-LAMP was assessed for its ability to detect M. tuberculosis complex in BACTEC MGIT 960-positive  cultures. Of the 103 cultures evaluated, 100 were identified to contain M. tuberculosis complex by  TB-LAMP and had concordant results with standard biochemical tests of niacin accumulation, nitrate  reductase, lack of heat-stable catalase, and susceptibility to para-nitrobenzoic acid. These results  indicate that TB-LAMP in combination with BACTEC MGIT 960 is a specific, reliable, and technically  feasible method for rapid and accurate identification of M. tuberculosis complex.
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
Janisara
J
Rudeeaneksin
Supranee
S
Bunchoo
Sopa
S
Srisungngam
Pathom
P
Sawanpanyalert
Sawet
S
Chamnangrom
Atipa
A
Kamolwat
Porntip
P
Thanasripakdeekul
Tooru
T
Taniguchi
Chie
C
Nakajima
Yasuhiko
Y
Suzuki
Benjawan
B
Phetsuksiri
D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis*
D012336:RNA, Ribosomal, 16S