[Modulatory effect of triptolide on differentiation of human Th17 cells].
OBJECTIVE
To investigate the effect of triptolide on the differentiation of human Th17 cells.


METHODS
Human peripheral blood mononuclear cells, purified CD4+ T cells and CD4+CD45RA- memory T cells were treated with various concentrations of triptolide in vitro. Cell proliferation was determined by MTT assay. Flow cytometry was used to analyze the intracellular expression of IL-17 and IFN-gamma. Cytokine production of IL-17 and IFN-gamma was measured by ELISA.


RESULTS
Cell proliferation, intracellular expression of IL-17 and IL-17 secretion were inhibited by triptolide in a dose-dependent manner. IFN-gamma expression and production were also inhibited by triptolide.


CONCLUSIONS
Triptolide inhibits the differentiation of human Th17 cell. The observation may indicate at least one of the mechanisms of the immunosuppressive and anti-inflammatory effects of triptolide.
Department of Dermatology, Sir Run Run Shaw Hospital, Medical School, Zhejiang University, Hangzhou 310016, China.
Pengyuan
P
Chen
Rui
R
Han
Qiang
Q
Zhou
Hao
H
Cheng
Kejian
K
Zhu
D000894:Anti-Inflammatory Agents, Non-Steroidal; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004224:Diterpenes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004852:Epoxy Compounds / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000187:drug effects / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism*; D010616:Phenanthrenes / Q000494:pharmacology*; D058504:Th17 Cells / Q000166:cytology* / Q000187:drug effects*; D029757:Tripterygium / Q000737:chemistry
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004224:Diterpenes; D004852:Epoxy Compounds; D020381:Interleukin-17; D010616:Phenanthrenes; C001899:triptolide; D007371:Interferon-gamma