Benefits of spine stabilization with biodegradable scaffolds in spinal cord injured rats.
Spine stabilization upon spinal cord injury (SCI) is a standard procedure in clinical practice, but rarely employed in experimental models. Moreover, the application of biodegradable biomaterials for this would come as an advantage as it would eliminate the presence of a nondegradable prosthesis within the vertebral bone. Therefore, in the present work, we propose the use of a new biodegradable device specifically developed for spine stabilization in a rat model of SCI. A 3D scaffold based on a blend of starch with polycaprolactone was implanted, replacing delaminated vertebra, in male Wistar rats with a T8-T9 spinal hemisection. The impact of spinal stabilization on the locomotor behavior was then evaluated for a period of 12 weeks. Locomotor evaluation--assessed by Basso, Beatie, and Bresnahan test; rotarod; and open field analysis--revealed that injured rats subjected to spine stabilization significantly improved their motor performance, including higher coordination and rearing activity when compared with SCI rats without stabilization. Histological analysis further revealed that the presence of the scaffolds not only stabilized the area, but also simultaneously prevented the infiltration of the injury site by connective tissue. Overall, these results reveal that SCI stabilization using a biodegradable scaffold at the vertebral bone level leads to an improvement of the motor deficits and is a relevant element for the successful treatment of SCI.
3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.
Nuno A
NA
Silva
Rui A
RA
Sousa
Joana S
JS
Fraga
Marco
M
Fontes
Hugo
H
Leite-Almeida
Rui
R
Cerqueira
Armando
A
Almeida
Nuno
N
Sousa
Rui L
RL
Reis
Antonio J
AJ
Salgado
D000818:Animals; D001672:Biocompatible Materials; D008297:Male; D009043:Motor Activity; D051381:Rats; D017208:Rats, Wistar; D045442:Rotarod Performance Test; D013119:Spinal Cord Injuries / Q000503:physiopathology*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials