Overexpression of Brassica rapa                      SHI-RELATED SEQUENCE genes suppresses growth and development in Arabidopsis thaliana.
S HI-R ELATED SEQUENCE (SRS) genes are plant-specific transcription factors containing a zinc-binding RING finger motif, which play a critical role in plant growth and development. We have characterized six SRS genes in Brassica rapa. Overexpression of the SRSs BrSTY1, BrSRS7, and BrLRP1 induced dwarf and compact plants, and significantly decreased primary root elongation and lateral root formation. Additionally, the transgenic plants had upward-curled leaves of narrow widths and with short petioles, and had shorter siliques and low fertility. In stems, hypocotyls, and styles, epidermal cell lengths were also significantly reduced in transgenic plants. RT-PCR analysis of transgenic plants revealed that BrSTY1, BrSRS7, and BrLRP1 regulate expression of several gibberellin (GA)- and auxin-related genes involved in morphogenesis in shoot apical regions. We conclude that BrSTY1, BrSRS7, and BrLRP1 regulate plant growth and development by regulating expression of GA- and auxin-related genes.
National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea. jkhongok@gmail.com
Joon Ki
JK
Hong
Jin A
JA
Kim
Jung Sun
JS
Kim
Soo In
SI
Lee
Bon Sung
BS
Koo
Yeon-Hee
YH
Lee
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029741:Brassica rapa / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000235:genetics / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D010641:Phenotype; D019441:Plant Epidermis / Q000378:metabolism; D010937:Plant Growth Regulators / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D005875:Gibberellins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors