A non-rotational, computer-controlled suspension bioreactor for expansion of umbilical cord blood mononuclear cells.
The proliferation and differentiation characteristics of umbilical cord blood mononuclear cells were examined in a non-rotational suspension bioreactor with a fishtail mixer. The system consisted of a glass vessel, a mixer that moved vertically, a data acquisition and control system to continuously monitor pH, temperature and dissolved O(2). The bioreactor provided superior expansion of total HSCs and not total cell number, as well as expression of stemness-related genes which followed with increasing in number of colony-forming cells during 14 days of culture compared to T -lask culture. Vertical agitation thus reduces the total cell number, which may be related to increased shear stress, but has no effect on HSC function.
Department of Regenerative Medicine, Cell Research Center, Royan Institute for Stem Cell Biology and Technology, Royan Cord Blood Bank, ACECR, Tehran, Iran. n.shayan@royaninstitute.org
Niloufar
N
Shayan
Marzieh
M
Ebrahimi
Bahareh
B
Beiki
Ehsan
E
Janzamin
D000704:Analysis of Variance; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D019149:Bioreactors; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000166:cytology*; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology
D015703:Antigens, CD; D015415:Biomarkers; D012333:RNA, Messenger