Influence of albumin on mineralization of PMMA-based/glass composites.
OBJECTIVE
The formation of a calcium phosphate layer on the surface of bone tissue engineering biomaterials is crucial for their integration in bone. Simulated biological fluids used to study the in vitro formation of those layers do not usually contain important organic components present in vivo-notably proteins. In this work the influence of bovine serum albumin on the mineralization process of poly(methylmethacrylate)-based composite was studied in vitro.


METHODS
The effect of protein on calcium phosphate formation was followed by ion concentration analyses (ICP), x-ray diffraction (XRD), and scanning electron microscopy coupled with x-ray energy dispersive spectroscopy (SEM-EDS).


CONCLUSIONS
The results showed the precipitation of a calcium phosphate layer on the surface of composites immersed in SBF and SBFA. Further transformation and crystallization of this layer, initially amorphous, appears to be influenced by the presence of albumin.
Centre for Research in Ceramic and Composite Materials (CICECO), Department of Ceramics and Glass Engineering, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
Bárbara J M Leite
BJ
Ferreira
Maria Gabriela G M
MG
Duarte
Maria Helena
MH
Gil
Maria Helena V
MH
Fernandes
Rui Nunes
RN
Correia
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000627:therapeutic use; D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry; D002417:Cattle; D003460:Crystallization; D005898:Glass / Q000737:chemistry*; D006801:Humans; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D023822:Tissue Engineering
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D012710:Serum Albumin, Bovine; D019904:Polymethyl Methacrylate; C020243:calcium phosphate