Determination of integrated HPV58 sequences in cervical lesions.
Integration of high-risk HPV in host genome is an important event in cervical cancer development. This study was aimed to analyze the integration of HPV58, a high-risk type that is prevalent in cervical lesions from southeastern Asia. Detection of integrated papillomavirus sequences by ligation-mediated polymerase chain reaction followed by DNA sequencing revealed 8 integrations in 5 samples, and virus integration was found present in 2 samples with early lesion. Sequence analysis of the viral-cellular junctions showed that E1 disruption in virus genome was an early and common event during HPV58 infection. In 6 integrations, DNA fragments of HPV58 genome integrated into the repetitive element sequences of host genome.
Microbial Genome Research Center, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Hui
H
Li
Ruifen
R
Zhang
Yupin
Y
Cai
Yuan
Y
Li
Xuemei
X
Cheng
Baoli
B
Zhu
Yi
Y
Yang
Yang
Y
Xiang
D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000821:virology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000235:genetics* / Q000821:virology; D016133:Polymerase Chain Reaction; D011379:Prognosis; D015996:Survival Rate; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology*; D016662:Virus Integration / Q000235:genetics*; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000821:virology
D004279:DNA, Viral