Isolation of fucoxanthin from edible brown algae by microwave-assisted extraction coupled with high-speed countercurrent chromatography.
A rapid and efficient method for the separation and purification of fucoxanthin from edible brown algae by microwave-assisted extraction coupled with high-speed countercurrent chromatography was developed. The algae were first extracted using microwave-assisted extraction, then the dried extract was dissolved and directly introduced into the high-speed countercurrent chromatography system with a two-phase solvent system consisting of hexane-ethyl acetate-ethanol-water (5:5:6:4, v/v/v/v). The isolation was done in less than 75 min, and a total of 0.83 mg, 1.09 mg, and 0.20 mg fucoxanthin were obtained from 25.0 g fresh Laminaria japonica Aresch, 1.5 g dry Undaria pinnatifida (Harv) Sur, and 15.0 g dry Sargassum fusiforme (Harv) Setch, respectively. The purity of fucoxanthin determined by HPLC was over 90% and its structure was further identified by LC-ESI-MS and (1) H-NMR.
School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, China.
Xiaohua
X
Xiao
Xiaoxi
X
Si
Zhiquan
Z
Yuan
Xianfang
X
Xu
Gongke
G
Li
D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry; D008872:Microwaves; D000459:Phaeophyceae / Q000737:chemistry*; D024341:Xanthophylls / Q000032:analysis / Q000302:isolation & purification*
D024341:Xanthophylls; C025164:fucoxanthin