High intensity exercise affects diurnal variation of some biological markers in trained subjects.
The study investigated if markers of muscle injury and antioxidant status were affected by a Wingate test performed at 2 different times of day. 15 young male footballers performed 2 tests (randomized) at 07:00-h and 17:00-h. Fasting blood samples were collected before and 3 min after each test for assessment of markers of muscle injury and antioxidant status. Resting oral temperature was recorded during each session. Peak power (10.76 ± 1.05 vs. 11.15 ± 0.83 W.kg( - 1)) and fatigue index (0.41 ± 0.04 vs. 0.49 ± 0.13%) during the Wingate test, and core temperature, were significantly higher (all p<0.05) in the evening. Markers of muscle injury were significantly higher in the evening before and after exercise (e. g., 148.7 ± 67.05 vs. 195 ± 74.6 and 191.6 ± 79.52 vs. 263.6 ± 96.06 IU.L (- 1), respectively, for creatine kinase; both p<0.001). Antioxidant parameters increased after the Wingate test but only resting values were significantly higher in the morning (e. g., 1.33 ± 0.19 vs. 1.19 ± 0.14 µmol.L (- 1) for total antioxidant status; p<0.05). The results indicate that muscle injury and antioxidant activity after the Wingate test were higher in the evening, suggesting a possible link between the biochemical measures and the diurnal fluctuation of anaerobic performance. However, repetition of this study after prescribed rather than self-selected exercise intensity is recommended.
Research Laboratory Sports performance optimization National Center of Medicine and Science in Sports (CNMSS), Tunis, Tunisia.
O
O
Hammouda
H
H
Chtourou
H
H
Chahed
S
S
Ferchichi
A
A
Chaouachi
C
C
Kallel
A
A
Miled
K
K
Chamari
N
N
Souissi
D000293:Adolescent; D000975:Antioxidants / Q000378:metabolism*; D056352:Athletes; D054874:Athletic Performance / Q000502:physiology; D001642:Bicycling / Q000502:physiology*; D015415:Biomarkers / Q000378:metabolism; D001831:Body Temperature; D002940:Circadian Rhythm / Q000502:physiology*; D005080:Exercise Test; D006801:Humans; D008297:Male; D018763:Muscle Fatigue / Q000502:physiology; D018482:Muscle, Skeletal / Q000293:injuries* / Q000502:physiology; D012916:Soccer; D013997:Time Factors
D000975:Antioxidants; D015415:Biomarkers