Kinetic study of biosurfactant production by Bacillus subtilis LAMI005 grown in clarified cashew apple juice.
In this work a low cost medium for the production of a biosurfactant by Bacillus subtilis LAMI005 and the kinetics of surfactin production considering the effect of initial substrate concentration were investigated. First, cashew apple juice supplementation for optimal production of biosurfactant by B. subtilis LAMI005 was studied. The medium formulated with clarified cashew apple juice and distilled water, supplemented with 1.0 g/L of (NH(4))(2)SO(4), proved to be the best among the nutrients evaluated. The crude biosurfactant had the ability to decrease the surface tension of water to 30 dyne/cm, with a critical micelle concentration (CMC) of 63.0 mg/L. Emulsification experiments indicated that this biosurfactant effectively emulsified kerosene (IE(24)=67%) and soybean oil (IE(24)=64%). Furthermore, the emulsion stability was always very high. It was shown by biochemical analysis, IR spectra, that there is no qualitative differences in the composition of the crude biosurfactant from a standard sample of surfactin from B. subtilis.
Universidade Federal do Ceará, Departamento de Engenharia Química, Campus do Pici, Fortaleza, CE, Brazil.
Darlane Wellen Freitas
DW
de Oliveira
Italo Waldimiro Lima
IW
França
Anne Kamilly Nogueira
AK
Félix
João Jeferson Lima
JJ
Martins
Maria Estela Aparecida
ME
Giro
Vânia Maria M
VM
Melo
Luciana Rocha Barros
LR
Gonçalves
D000465:Algorithms; D000645:Ammonium Sulfate / Q000737:chemistry; D031021:Anacardium / Q000737:chemistry*; D001412:Bacillus subtilis / Q000737:chemistry / Q000378:metabolism*; D018533:Biomass; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004655:Emulsions; D005285:Fermentation; D005638:Fruit / Q000737:chemistry; D007700:Kinetics; D055666:Lipopeptides / Q000737:chemistry; D008823:Micelles; D010456:Peptides, Cyclic / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013500:Surface Tension; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism*
D002241:Carbohydrates; D003470:Culture Media; D004655:Emulsions; D055666:Lipopeptides; D008823:Micelles; D010456:Peptides, Cyclic; D013501:Surface-Active Agents; C009365:surfactin peptide; D000645:Ammonium Sulfate