An efficient way from naringenin to carthamidine and isocarthamidine by Aspergillus niger.
Biotransformation of naringenin with Aspergillus niger CGMCC 3.4628 yielded two hydroxylation products which were identified unambiguously as 6-hydroxylnaringenin (carthamidin) and 8-hydroxylnaringenin (isocarthamidin) by ESI-MS and (1)H-NMR. Both products simultaneously arrived at high level after 48 h in the biotransformation process. The highest conversion efficiency of carthamidin was 0.38 mg/mg of naringenin and that of isocarthamidin was 0.43 mg/mg of naringenin. Antioxidant property assay using a thin layer chromatography-bioautographic-based DPPH scavenging method demonstrated that both hydroxylation metabolites exhibited much stronger activity than naringenin. The high efficiency and convenient procedure makes the biotransformation with A. niger described in current work a potential way to produce carthamidin and isocarthamidin.
The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines and the MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201210, China.
Jing
J
Xu
Li
L
Yang
Shu-Juan
SJ
Zhao
Zheng-Tao
ZT
Wang
Zhi-Bi
ZB
Hu
D000975:Antioxidants / Q000378:metabolism*; D001234:Aspergillus niger / Q000378:metabolism*; D001711:Biotransformation; D044950:Flavanones / Q000378:metabolism*; D006900:Hydroxylation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013997:Time Factors; D047310:Apigenin
D000975:Antioxidants; D044950:Flavanones; C005273:naringenin; C458179:scutellarein; D047310:Apigenin