Analysis and quantification of ammonia-oxidizing bacteria community with amoA gene in sewage treatment plants.
The analysis and quantification of ammonia-oxidizing bacteria (AOB) is crucial, as they initiate the biological removal of ammonia-nitrogen from sewage. Previous methods for analyzing the microbial community structure, which involve the plating of samples or culture media over agar plates, have been inadequate because many microorganisms found in a sewage plant are unculturable. In this study, to exclusively detect AOB, the analysis was carried out via denaturing gradient gel electrophoresis using a primer specific to the amoA gene, which is one of the functional genes known as ammonia monooxygenase. An AOB consortium (S1 sample) that could oxidize an unprecedented 100% of ammonia in 24 h was obtained from sewage sludge. In addition, real-time PCR was used to quantify the AOB. Results of the microbial community analysis in terms of carbon utilization ability of samples showed that the aeration tank water sample (S2), influent water sample (S3), and effluent water sample (S4) used all the 31 substrates considered, whereas the AOB consortium (S1) used only Tween 80, D-galacturonic acid, itaconic acid, D-malic acid, and L-serine after 192 h. The largest concentration of AOB was detected in S1 (7.6 × 10(6) copies/microliter), followed by S2 (3.2 × 10(6) copies/microliter), S4 (2.8 × 10(6) copies/microliter), and S3 (2.4 × 10(6) copies/microliter).
Department of Environmental and Energy Engineering, The University of Suwon, Gyeonggi 445-743, Korea.
Sun Hwa
SH
Hong
Hyun Duck
HD
Jeong
Bongjin
B
Jung
Eun Young
EY
Lee
D000641:Ammonia / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005798:Genes, Bacterial; D006603:Hexuronic Acids / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D059013:Microbial Consortia / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D011136:Polysorbates / Q000737:chemistry; D025341:Principal Component Analysis; D060888:Real-Time Polymerase Chain Reaction; D012037:Refuse Disposal / Q000379:methods*; D012694:Serine / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D013386:Succinates / Q000378:metabolism; D013501:Surface-Active Agents / Q000737:chemistry
D004269:DNA, Bacterial; D006603:Hexuronic Acids; D008293:Malates; D011136:Polysorbates; D012722:Sewage; D013386:Succinates; D013501:Surface-Active Agents; D012694:Serine; C007819:galacturonic acid; D000641:Ammonia; C030298:malic acid; D010088:Oxidoreductases; C045582:ammonia monooxygenase; C005229:itaconic acid