Design of tissue engineering implants for bone tissue regeneration of the basis of new generation polylactoglycolide scaffolds and multipotent mesenchymal stem cells from human exfoliated deciduous teeth (SHED cells).
Cultures of multipotent mesenchymal stromal cells from the pulp of human deciduous teeth (SHED cells) were characterized. The cells were used for population of 3D biodegradable polylactoglycolide scaffolds; their osteogenic potential was preserved under these conditions. Implantation of the scaffolds to mice induced no negative reactions in the recipients. These results suggest that the use of polylactoglycolide scaffolds populated with SHED cells is a promising approach for creation of implants for bone defect replacement.
V. N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia. vakhrunya@gmail.com
I V
IV
Vakhrushev
E N
EN
Antonov
A V
AV
Popova
E V
EV
Konstantinova
P A
PA
Karalkin
I V
IV
Kholodenko
A Yu
AY
Lupatov
V K
VK
Popov
V N
VN
Bagratashvili
K N
KN
Yarygin
D000818:Animals; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D039902:Multipotent Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014094:Tooth, Deciduous / Q000166:cytology*