Influence of temperature and salinity on microbial structure of marine anammox bacteria.
Anaerobic ammonium oxidation (anammox) is a type of biological oxidation mediated by a group of Planctomycete-like bacteria. Members of the genus Candidatus Scalindua are mainly found in marine environments, but not exclusively. This group is cultured using different inoculums and conditions; however, its optimal growth conditions are not clear. Additionally, little information is known about the factors that influence the activity and the selection of a population of marine anammox bacteria. This study was conducted to investigate the influence of temperature and salinity on the marine anammox community. To accomplish this, an up-flow fixed-bed column reactor was operated, and quantitative fluorescence in situ hybridization (FISH) with probes specific to dominant marine anammox bacteria was conducted. Anammox activity was observed at 20 and 30 °C, but not at 10 °C. A nitrogen removal rate of 0.32 kg TN m(-3) day(-1) was obtained at 20 °C. These results suggest that temperature affects the activity (nitrogen removal rate) of anammox bacteria, while salinity does not affect the activity in the marine anammox biofilm.
Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashihiroshima 739-8527, Japan.
Takanori
T
Awata
Katsuichiro
K
Tanabe
Tomonori
T
Kindaichi
Noriatsu
N
Ozaki
Akiyoshi
A
Ohashi
D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism*; D019149:Bioreactors; D017404:In Situ Hybridization, Fluorescence; D009584:Nitrogen / Q000378:metabolism*; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D054712:Salinity; D013696:Temperature; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism; D018508:Water Purification
D000644:Quaternary Ammonium Compounds; D014874:Water Pollutants, Chemical; D009584:Nitrogen