Increased norepinephrine by medium-chain triglyceride attributable to lipolysis in white and brown adipose tissue of C57BL/6J mice.
A further investigation of the lipolysis induced by medium-chain triglyceride (MCT) was conducted on C57BL/6J mice fed with a diet containing 2% MCT or 2% long-chain triglyceride (LCT). Blood norepinephrine, body fat and blood lipid variables, and the protein or mRNA expression of the genes relevant to lipolysis were measured and analyzed in the white and brown adipose tissue (WAT, BAT). Decreased body fat and improved blood lipid profiles attributable to MCT were confirmed. A higher level of blood norepinephrine was observed with the MCT diet. The adipose triglyceride lipase (ATGL) activity and its mRNA expression, the expression of protein and mRNA of the beta 3 adrenergic receptor (β3-AR) in both WAT and BAT, and the hormone-sensitive lipase (HSL) activity and its mRNA expression in BAT were significantly increased in the mice with MCT feeding. The lipolysis induced by MCT might be partially mediated by increasing norepinephrine, thereafter signaling the up-regulation of β3-AR, ATGL, and HSL in WAT and BAT.
Department of Nutrition, Chinese PLA General Hospital, and Peking University Health Science, Beijing, China.
Ying-hua
YH
Liu
Yong
Y
Zhang
Qing
Q
Xu
Xiao-ming
XM
Yu
Xin-sheng
XS
Zhang
Jin
J
Wang
Chao
C
Xue
Xue-yan
XY
Yang
Rong-xin
RX
Zhang
Chang-yong
CY
Xue
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage; D015870:Gene Expression / Q000187:drug effects; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000097:blood*; D012333:RNA, Messenger / Q000096:biosynthesis; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D002787:Sterol Esterase / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship; D014280:Triglycerides / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D000217:Acyltransferases
D004041:Dietary Fats; D008049:Lipase; D009638:Norepinephrine; D012333:RNA, Messenger; D022702:Receptors, Adrenergic, beta-3; D002787:Sterol Esterase; D014280:Triglycerides; C494445:PNPLA2 protein, mouse; D000217:Acyltransferases