Association between HLA-DQ genotypes and haplotypes vs Helicobacter pylori infection in an Indonesian population.
BACKGROUND
Helicobacter pylori is an important gastrointestinal pathogen related to the development of not only atrophic gastritis and peptic ulcer, but also gastric cancer. Human leukocyte antigens (HLA) may play particular roles in host immune responses to bacterial antigens. This study aimed to investigate the association between HLA-DQA1 and DQB1 genotypes and haplotypes vs H. pylori infection in an Indonesian population.


METHODS
We selected 294 healthy participants in Mataram, Lombok Island, Indonesia. H. pylori infection was determined by urea breath test (UBT). We analyzed HLA-DQA1 and DQB1 genotypes by PCR-RFLP and constructed haplotypes of HLA-DQA1 and DQB1 genes. Multiple comparisons were conducted according to the Bonferroni method.


RESULTS
The H. pylori infection rate was 11.2% in this Indonesian population. The DQB1*0401 genotype was noted to be associated with a high risk of H. pylori infection, compared with the DQB1*0301 genotype. None of the HLA-DQA1 or DQB1 haplotypes were related to the risk of H. pylori infection.


CONCLUSIONS
The study suggests that HLADQB1 genes play important roles in H. pylori infection, but there was no statistically significant association between HLA-DQA1 or DQB1 haplotypes and H.pylori infection in our Lombok Indonesian population.
Department of Public Health, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Yang
Y
Zhao
Jingwen
J
Wang
Tsutomu
T
Tanaka
Akihiro
A
Hosono
Ryosuke
R
Ando
Soeripto
S
Soeripto
F X
FX
Ediati Triningsih
Tegu
T
Triono
Suwignyo
S
Sumoharjo
E Y Wenny
EY
Astuti
Stephanus
S
Gunawan
Shinkan
S
Tokudome
D000293:Adolescent; D000328:Adult; D000368:Aged; D016009:Chi-Square Distribution; D002648:Child; D005260:Female; D005838:Genotype; D059848:HLA-DQ alpha-Chains / Q000235:genetics*; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D006239:Haplotypes; D016481:Helicobacter Infections / Q000235:genetics*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007214:Indonesia; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen