Enhancement of diepoxin ζ production in liquid culture of endophytic fungus Berkleasmium sp. Dzf12 by polysaccharides from its host plant Dioscorea zingiberensis.
This study is the first report of the enhancement of diepoxin ζ production in liquid culture of the endophytic fungus Berkleasmium sp. Dzf12 by the polysaccharides from its host plant Dioscorea zingiberensis which serve as elicitors. Three polysaccharides, namely water-extracted polysaccharide (WEP), sodium hydroxide-extracted polysaccharide and acid-extracted polysaccharide were sequentially prepared from the rhizomes of D. zingiberensis. Among them, WEP was found to be the most effective elicitor to enhance diepoxin ζ production. When WEP was added to the medium at 400 mg l(-1) on day 3 of culture, the maximal diepoxin ζ yield (intracellular diepoxin ζ in mycelia plus extracellular diepoxin ζ in medium) of 350.76 mg l(-1) on day 15 was achieved, which was about 2.69-fold in comparison with that (130.43 mg l(-1)) of the control.
College of Biology and Science, Sichuan Agricultural University, Yaan, 625014, China.
Yan
Y
Li
Peiqin
P
Li
Yan
Y
Mou
Jianglin
J
Zhao
Tijiang
T
Shan
Chunbang
C
Ding
Ligang
L
Zhou
D001203:Ascomycota / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D027742:Dioscorea / Q000737:chemistry*; D060026:Endophytes / Q000187:drug effects / Q000378:metabolism; D004852:Epoxy Compounds / Q000378:metabolism*; D011134:Polysaccharides / Q000302:isolation & purification / Q000378:metabolism*
D003470:Culture Media; D004852:Epoxy Compounds; D011134:Polysaccharides