Altered ganglioside GD3 in HeLa cells might influence the cytotoxic abilities of NK cells.
OBJECTIVE
Previously, we found that altered sialidases in HeLa cells in a natural killer-HeLa (NK-HeLa) coculture system contributed to the decreased cytotoxic ability of NK cells. However, changes that occur in the glycosylation of the HeLa cells in the NK-HeLa coculture system remain unknown.


METHODS
An NK-HeLa coculture system was used to examine the changes that occur in the gangliosides of HeLa cells.


RESULTS
GD3 expression in HeLa cells was significantly increased in the NK-HeLa coculture system. Exogenous ganglioside GD3 decreased the cytotoxic ability of the NK cells, which could be restored by the addition of the anti-GD3 antibody. Coadministration of GD3 and sialidase further decreased the cytotoxic ability of the NK cells, which could be partially restored by the addition of a sialidase inhibitor (DANA). GD3 expression in HeLa cells also decreased following DANA treatment.


CONCLUSIONS
This study suggests that interactions between ganglioside GD3 and sialidases in HeLa cells influence the cytotoxic ability of NK cells.
Department of Obstetrics and Gynecology, National Yang-Ming University School of Medicine, Taipei, Taiwan.
Wen-Chi
WC
Lee
Wen-Ling
WL
Lee
Wen-Yuann
WY
Shyong
Lin-Wei
LW
Yang
Min-Chun
MC
Ko
Chang-Ching
CC
Yeh
Shie-Liang Edmond
SL
Hsieh
Peng-Hui
PH
Wang
D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005732:Gangliosides / Q000378:metabolism* / Q000494:pharmacology; D006367:HeLa Cells / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007108:Immune Tolerance; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D009439:Neuraminidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013400:Sugar Acids / Q000494:pharmacology; D015854:Up-Regulation
C577845:5-acetamido-2,6-anhydro-2,3-dehydro-3,5-dideoxy-glycerogalactononanoic acid; D005732:Gangliosides; D013400:Sugar Acids; C026226:ganglioside, GD3; D009439:Neuraminidase