Characteristics of aerobic and anaerobic ammonium-oxidizing bacteria in the hyporheic zone of a contaminated river.
Both β-proteobacterial aerobic ammonium-oxidizing bacteria (AOB) and anaerobic ammonium-oxidizing (ANAMMOX) bacteria were investigated in the hyporheic zone of a contaminated river in China containing high ammonium levels and low chemical oxygen demand. Fluorescence in-situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE) and cloning-sequencing were employed in this study. FISH analysis illustrated that AOB (average population of 3.5 %) coexisted with ANAMMOX bacteria (0.7 %). The DGGE profile revealed a high abundance and diversity of bacteria at the water-air-soil interface rather than at the water-soil interface. The redundancy analysis correlated analysis showed that the diversity of ANAMMOX bacteria was positively related to the redox potential. The newly detected sequences of ANAMMOX organisms principally belonged to the genus Candidatus "Brocadia", while most ammonia monooxygenase subunit-A gene amoA sequences were affiliated with Nitrosospira and Nitrosomonas. These results suggest that the water-air-soil interface performs an important function in the nitrogen removal process and that the bioresources of AOB and ANAMMOX bacteria can potentially be utilized for the eutrophication of rivers.
MOE Key Laboratory of Water and Sediment Sciences, School of Environment, Beijing Normal University, Beijing, 100875, China.
Ziyuan
Z
Wang
Yun
Y
Qi
Jun
J
Wang
Yuansheng
Y
Pei
D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D044822:Biodiversity; D002681:China; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005798:Genes, Bacterial; D017404:In Situ Hybridization, Fluorescence; D009606:Nitrosomonas / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D000644:Quaternary Ammonium Compounds / Q000032:analysis / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017423:Sequence Analysis, RNA; D014871:Water Microbiology
D004269:DNA, Bacterial; D000644:Quaternary Ammonium Compounds; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C045582:ammonia monooxygenase