Characterization of LHY-821, a novel moderately differentiated endometrial carcinoma cell line.
Endometrial cancer is a major problem for women but only a small number of comprehensively characterized cell models are available for studies. Here, we established a new cell line derived from a Stage IIIc(1) Grade 2 endometrial adenocarcinoma. The cell line, designated LHY-821, was characterized using growth curve, karyotyping, immunohistochemical staining, immunoblotting, drug sensitivity assay, invasion assay, and xenografting in nude mice. LHY-821 has a doubling time of about 46 h and a colony-forming efficiency of approximately 71 %. These cells expresse high levels of progesterone receptor but not estrogen receptor and are sensitive to medroxyprogesterone acetate (MPA). LHY-821 also expresses pan-cytokeratin, PTEN, p53, β-catenin, IGF-1, and IGF-2. In addition, karyotype analysis revealed that LHY-821 possessed a near diploid karyotype including 6q-, 10p-, Xq-, 13q+, 17p+, and Triplo-12. LHY-821 showed highly tumorigenicity in nude mice (100 %) and weak invasiveness. Chemosensitivity tests showed that LHY-821 was sensitive to both carboplatin and paclitaxel. LHY-821 is an immortalized cell line which had survived more than 80 serial passages; it may provide a novel tool to study the molecular mechanism and potential treatment for endometrial cancer.
Department of Obstetrics and Gynecology, First Affiliated Hospital of Peking University, West District, Beijing, China.
Qian
Q
Hu
Li
L
Yu
Rui
R
Chen
Yan
Y
Zhang
Ya
Y
Xie
Qinping
Q
Liao
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor / Q000166:cytology* / Q000502:physiology; D016889:Endometrial Neoplasms; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D007621:Karyotyping; D017258:Medroxyprogesterone Acetate; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D011980:Receptors, Progesterone / Q000378:metabolism; D014183:Transplantation, Heterologous / Q000502:physiology
D011980:Receptors, Progesterone; D017258:Medroxyprogesterone Acetate