Separomics applied to the proteomics and peptidomics of low-abundance proteins: Choice of methods and challenges - A review.
The enrichment and isolation of proteins are considered limiting steps in proteomic studies. Identification of proteins whose expression is transient, those that are of low-abundance, and of natural peptides not described in databases, is still a great challenge. Plant extracts are in general complex, and contaminants interfere with the identification of proteins involved in important physiological processes, such as plant defense against pathogens. This review discusses the challenges and strategies of separomics applied to the identification of low-abundance proteins and peptides in plants, especially in plants challenged by pathogens. Separomics is described as a group of methodological strategies for the separation of protein molecules for proteomics. Several tools have been used to remove highly abundant proteins from samples and also non-protein contaminants. The use of chromatographic techniques, the partition of the proteome into subproteomes, and an effort to isolate proteins in their native form have allowed the isolation and identification of rare proteins involved in different processes.
Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Maria Cristina
MC
Baracat-Pereira
Meire
M
de Oliveira Barbosa
Marcos Jorge
MJ
Magalhães
Lanna Clicia
LC
Carrijo
Patrícia Dias
PD
Games
Hebréia Oliveira
HO
Almeida
José Fabiano
JF
Sena Netto
Matheus Rodrigues
MR
Pereira
Everaldo Gonçalves
EG
de Barros