Regenerative treatment using a radioelectric asymmetric conveyor as a novel tool in antiaging medicine: an in vitro beta-galactosidase study.
BACKGROUND
Beta-galactosidase is the most widely used biomarker for highlighting the processes of cellular aging, including neurodegeneration. On this basis, we decided to test in vitro whether a set of rescuing/reparative events previously observed by us in subjects treated with radioelectric asymmetric conveyor (REAC) technology may also involve antagonism of a marker of aging-related degenerative processes, as assessed by a reduction in beta-galactosidase at the cellular level.


METHODS
Human adipose-derived stem cells were cultured at different passages, ranging from 5 to 20, with or without REAC exposure for 12 hours. The cells were then processed for biochemical beta-galactosidase staining and morphological microscopy analysis.


RESULTS
We observed a significant reduction in expression of senescence associated-beta-galactosidase, and a persistence of fibroblast-like morphology typical of human adipose-derived stem cells, even at late passages.


CONCLUSIONS
Our results indicate the ability of REAC technology to counteract in vitro senescence of human adipose-derived stem cells, and prompt the hypothesis that such technology may be exploited to antagonize in vivo senescence of tissue-resident or transplanted stem cells playing an important role in clinical treatment of age-related processes.
Department of Regenerative Medicine, Rinaldi Fontani Institute, Florence. srinaldi@irf.it
Salvatore
S
Rinaldi
Margherita
M
Maioli
Sara
S
Santaniello
Alessandro
A
Castagna
Gianfranco
G
Pigliaru
Sara
S
Gualini
Matteo Lotti
ML
Margotti
Arturo
A
Carta
Vania
V
Fontani
Carlo
C
Ventura
D000273:Adipose Tissue / Q000166:cytology; D000375:Aging / Q000502:physiology*; D015415:Biomarkers / Q000032:analysis; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D004558:Electric Stimulation; D006801:Humans; D019636:Neurodegenerative Diseases / Q000517:prevention & control; D011846:Radio Waves; D012038:Regeneration / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology; D001616:beta-Galactosidase / Q000302:isolation & purification* / Q000502:physiology
D015415:Biomarkers; D001616:beta-Galactosidase