[The relationship between oxidative stress and endothelial progenitor cells count in the first-degree relatives of diabetes mellitus].
OBJECTIVE
To study the relationship between oxidative stress and endothelial progenitor cells (EPCs) count in the first-degree relatives of diabetes mellitus (FDRs).


METHODS
Three groups were evaluated with 40 type 2 diabetes mellitus (T2DM) patients, 38 FDRs and 30 healthy individuals as the control (NC). Fasting plasma glucose (FPG), glycosylated hemoglobin (HbA1c), TG, TC and fasting plasma insulin concentrations were measured and homeostasis model assessment-insulin resistance (HOMA-IR) was calculated. Quantity of EPCs and flow-mediated dilation (FMD) were evaluated. Malonaldehyde (MDA), glutathion peroxidase (GSH-Px), erythrocuprein (SOD) and total anti-oxidative capacity (TAO-C) were measured.


RESULTS
In T2DM group FPG [(7.86 ± 0.77) mmol/L] and HbA1c [(7.24 ± 0.20) %] were significantly higher than those in NC [FPG (4.90 ± 0.35) mmol/L, HbA1c (5.34 ± 0.37)%] and FDRs group [FPG (5.13 ± 0.95) mmol/L, HbA1c (5.36 ± 0.36)%] (all P values < 0.05). TC in T2DM group [(5.88 ± 0.76) mmol/L] was higher than in NC [(4.66 ± 0.90) mmol/L] and FDRs [(4.95 ± 0.76) mmol/L]. HOMA-IR was 0.48 ± 0.25 in NC, 0.81 ± 0.46 in FDRs and 1.47 ± 0.24 in T2DM group, P < 0.01. In T2DM group, the plasma levels of SOD [(69.30 ± 2.21) U/ml], TAO-C [(7.30 ± 0.29) U/ml] and GSH-Px [(856.5 ± 9.01) U/ml] were significantly lower than those in NC [SOD (75.33 ± 3.63) U/ml, TAO-C (8.17 ± 0.58) U/ml and GSH-Px (938.1 ± 19.35) U/ml] and FDRs group [SOD (74.91 ± 4.53) U/ml, TAO-C (8.24 ± 0.46) U/ml and GSH-Px (936.9 ± 15.78) U/ml] (all P values < 0.01). Serum level of MDA was (2.87 ± 0.63) µmol/L in NC, (3.28 ± 0.71) µmol/L in FDRs and (3.69 ± 0.39) µmol/L in T2DM group (P < 0.01). The quantity of EPCs and FMD% were 96.75 ± 8.11 and 8.36 ± 2.21 in NC, 83.34 ± 12.43 and 6.78 ± 0.98 in FDRs and 58.45 ± 7.58 and 2.86 ± 0.35 in T2DM group with statistical differences between different groups (all P values < 0.05). Pearson correlation analysis showed that lnHOMA-IR was positively correlated with MDA (r = 0.486, P < 0.05) and negatively correlated with SOD, TAO-C, GSH-Px (r = -0.426, -0.601, -0.524, all P values < 0.05) in FDRs group.


CONCLUSIONS
Insulin resistance, oxidative stress, decreased quantity of EPCs and impairment of endovascular function have already occurred in the FDRs of T2DM with normal glucose tolerance and they are correlated with each other.
The First Department of Endocrinology, Hebei General Hospital, Shijiazhuang 050051, China.
Shu-chun
SC
Chen
Guang-yao
GY
Song
Yang
Y
Sun
Na
N
Liu
D000328:Adult; D001786:Blood Glucose; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D013234:Stem Cells / Q000166:cytology
D001786:Blood Glucose; D007328:Insulin