Proteomics in studying cancer stem cell biology.
Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. However, very little is known about the regulation of SC maintenance pathways in cancer and how these are affected by cancer-specific genetic alterations and by treatment. Proteomics is emerging as a powerful tool to identify the signaling complexes and pathways that control multi- and pluri-potency and SC differentiation. Here, the authors review the novel insights that these studies have provided and present a comprehensive strategy for the use of proteomics in studying cancer SC biology.
Department of Surgery, University Medical Center Utrecht, Heidelberglaan 100, 3584CX, The Netherlands. o.kranenburg@umcutrecht.nl
Onno
O
Kranenburg
Benjamin L
BL
Emmink
Jaco
J
Knol
Winan J
WJ
van Houdt
Inne H M Borel
IH
Rinkes
Connie R
CR
Jimenez
D014408:Biomarkers, Tumor / Q000032:analysis*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D039902:Multipotent Stem Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000502:physiology
D014408:Biomarkers, Tumor