Protocol for the detection of Treponema pallidum in paraffin-embedded specimens.
Formalin-fixed paraffin-embedded (FFPE) tissue blocks are routinely used for histopathological examination and are also useful for specific pathogen detection by polymerase chain reaction (PCR). FFPE tissue is stable at ambient temperature for an extended period of time and relatively easy to transport compared to fresh tissue, which has to be processed or frozen immediately. In addition, archival material is an invaluable source for retrospective molecular and clinical investigation. This chapter describes detailed procedures for nucleic acid extraction and PCR detection of Treponema pallidum using FFPE tissue.
Laboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA. cychen@cdc.gov
Cheng-Yen
CY
Chen
Allan
A
Pillay
D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005557:Formaldehyde / Q000378:metabolism; D006801:Humans; D025202:Molecular Diagnostic Techniques; D016612:Paraffin Embedding / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D016707:Tissue Fixation; D014210:Treponema pallidum / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D005557:Formaldehyde