Leukotrienes B4, C4, D4 and E4 in the exhaled breath condensate (EBC), blood and urine in patients with pneumoconiosis.
Leukotrienes (LTs) are involved in the pathogenesis of lung fibrosis and were increased in exhaled breath condensate (EBC) of the patients with pneumoconiosis. However the possible influence of extra-pulmonary disorders on the EBC markers is not known. Therefore in parallel with EBC, LTs' levels in the plasma and urine were measured in patients with pneumoconiosis (45 × asbestos exposure, 37 × silica exposure) and in 27 controls. Individual LTs B4, C4, D4 and E4 were measured by liquid chromatography - electrospray ionization - tandem mass spectrometry (LC-ESI-MS/MS). In EBC, LT D4 and LT E4 were increased in both groups of patients (p<0.001 and p<0.05), comparing with the controls. Both LT B4 and cysteinyl LTs were elevated in asbestos-exposed subjects (p<0.05). Asbestosis with more severe radiological signs (s1/s2-t3/u2) and lung functions impairment has shown higher cysteinyl LTs and LT C4 in the EBC (p<0.05) than mild asbestosis (s1/s0-s1/s1). In addition, in the subjects with asbestosis, cysteinyl LTs in EBC correlated with TLC (-0.313, p<0.05) and TLCO/Hb (-0.307, p<0.05), and LT C4 with TLC (-0.358, p<0.05). In pneumoconioses, EBC appears the most useful from the 3 fluids studied.
Department of Occupational Medicine of the 1st Faculty of Medicine, Charles University in Prague, Czech Republic. daniela.pelclova@LF1.cuni.cz
Daniela
D
Pelclová
Zdenka
Z
Fenclová
Stepánka
S
Vlcková
Jindriška
J
Lebedová
Kamila
K
Syslová
Ondrej
O
Pecha
Jaromír
J
Belácek
Tomáš
T
Navrátil
Marek
M
Kuzma
Petr
P
Kacer
D000368:Aged; D001195:Asbestosis / Q000000981:diagnostic imaging / Q000378:metabolism*; D001944:Breath Tests; D005260:Female; D006801:Humans; D007975:Leukotriene B4 / Q000032:analysis / Q000097:blood / Q000652:urine; D017997:Leukotriene C4 / Q000032:analysis / Q000097:blood / Q000652:urine; D017998:Leukotriene D4 / Q000032:analysis / Q000097:blood / Q000652:urine; D017999:Leukotriene E4 / Q000032:analysis / Q000097:blood / Q000652:urine; D015289:Leukotrienes / Q000032:analysis* / Q000097:blood / Q000652:urine; D008297:Male; D008875:Middle Aged; D011859:Radiography; D012129:Respiratory Function Tests; D012720:Severity of Illness Index; D012829:Silicosis / Q000000981:diagnostic imaging / Q000378:metabolism*
D015289:Leukotrienes; D007975:Leukotriene B4; D017997:Leukotriene C4; D017998:Leukotriene D4; D017999:Leukotriene E4