[Neuroprotective effect of longistyline A against corticosterone-induced neurotoxicity in PC12 cells].
This study is to investigate the protective effect of longistyline A against corticosterone-induced neurotoxicity in PC12 cells. While PC12 cells were exposed to 100 micromol x L(-1) corticosterone for 48 h, cell survival rate was reduced and lactate dehydrogenase (LDH) release increased. In parallel, corticosterone caused significant elevations of DNA fragmentation, [Ca2+]i and caspase-3 activity. However, when the PC12 cells were incubated with longistyline A (4.0, 8.0 and 16.0 micromol x L(-1)) in the presence of 100 micromol x L(-1) corticosterone for 48 h, the effects were evidently alleviated, but dose-dependent manner was not obvious. In summary, longistyline A could generate a neuroprotective effect against corticosterone-induced neurotoxicity in PC12 cells possibly by decreasing [Ca2+]i and caspase-3 activity.
Sichuan Agricultural University, Ya 'an 625014, China.
Bao-Ping
BP
Jiang
Rui-Wu
RW
Yang
Xin-Min
XM
Liu
Ya-Min
YM
Liu
Qi
Q
Chang
Jian-Yong
JY
Si
Rui-Le
RL
Pan
D000818:Animals; D036561:Cajanus / Q000737:chemistry; D002118:Calcium / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects*; D003345:Corticosterone / Q000633:toxicity; D053938:DNA Fragmentation / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016716:PC12 Cells; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats
D018696:Neuroprotective Agents; D010636:Phenols; C583590:longistyline A; D007770:L-Lactate Dehydrogenase; D053148:Caspase 3; D002118:Calcium; D003345:Corticosterone