Computational selection, identification and structural analysis of ω-aminotransferases with various substrate specificities from the genome sequence of Mesorhizobium loti MAFF303099.
ω-Aminotransferase (ω-AT) is an important class of enzymes for the synthesis of chiral amines or β-amino acids. Family profile analysis was applied to screen putative ω-ATs from Mesorhizobium loti MAFF303099, a nitrogen fixation bacterium that has a larger number of ATs than other microorganisms. By family profile analysis, we selected 10 putative ω-ATs according to E-value. The functions of the putative ω-ATs were investigated by examining activities towards amines and/or β-amino acids. 10 putative proteins were found to have ω-AT activity with narrow or broad substrate specificity. Structure analysis using crystal structure of mll7127 and homology models of mll1632 and mll3663 indicated that the structures of active sites of the enzymes were very similar and highly conserved, but their substrate specificities appeared to be determined by residues positioned at the entrance region of the active site binding pockets.
School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
Joo-Hyun
JH
Seo
Joon-Young
JY
Hwang
Su-Hyun
SH
Seo
Hyunjong
H
Kang
Bum-Yeol
BY
Hwang
Byung-Gee
BG
Kim
D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D002874:Chromosome Mapping; D019295:Computational Biology; D018360:Crystallography, X-Ray; D016680:Genome, Bacterial; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D059128:Mesorhizobium / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D008958:Models, Molecular; D005810:Multigene Family; D016297:Mutagenesis, Site-Directed; D009586:Nitrogen Fixation / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040681:Structural Homology, Protein; D013379:Substrate Specificity; D000637:Transaminases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D007527:Isoenzymes; D011994:Recombinant Proteins; D000637:Transaminases