Application of low-vacuum scanning electron microscopy for renal biopsy specimens.
Low-vacuum scanning electron microscopy (LV-SEM) has been developed which enables the observation of soft, moist, and electrically insulating materials without any pretreatment unlike conventional scanning electron microscopy, in which samples must be solid, dry and usually electrically conductive. The purpose of this study was to assess the usefulness of LV-SEM for renal biopsy specimens. We analyzed 20 renal biopsy samples obtained for diagnostic purposes. The sections were stained with periodic acid methenamine silver to enhance the contrast, and subsequently examined by LV-SEM. LV-SEM showed a precise and fine structure of the glomerulus in both formalin fixed paraffin and glutaraldehyde-osmium tetroxide-fixed epoxy resin sections up to 10,000-fold magnification. The spike formation on the basement membrane was clearly observed in the membranous nephropathy samples. Similarly to transmission electron microscopy, electron dense deposits were observed in the epoxy resin sections of the IgA nephropathy and membranous nephropathy samples. LV-SEM could accurately show various glomerular lesions at high magnification after a simple and rapid processing of the samples. We consider that this is a novel and useful diagnostic tool for renal pathologies.
Department of Pathology, The University of Tokyo Hospital, Tokyo, Japan. hirokim@cc.saga-u.ac.jp
Hiroki
H
Miyazaki
Hiroshi
H
Uozaki
Akihiro
A
Tojo
Sayuri
S
Hirashima
Sumire
S
Inaga
Kei
K
Sakuma
Yasuyuki
Y
Morishita
Masashi
M
Fukayama
D001707:Biopsy, Needle; D050533:Glomerular Basement Membrane / Q000648:ultrastructure; D006801:Humans; D007668:Kidney / Q000648:ultrastructure*; D007674:Kidney Diseases / Q000175:diagnosis*; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D046529:Microscopy, Electron, Transmission / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D013194:Staining and Labeling; D016707:Tissue Fixation; D014618:Vacuum