Understanding the cell behavior on nano-/micro-patterned surfaces.
OBJECTIVE
This article reports on studies conducted in the same laboratory on interactions between patterned substrates with different pattern dimensions and chemistries, and various types of cells.


METHODS
In order to compare the influence of various parameters, bone marrow stromal cells, retinal pigment epithelial cells, human corneal stromal cells (keratocytes), Saos-2 (human osteosarcoma cells), human microvascular endothelial cells and vascular smooth muscle cells were tested on surfaces with different physical patterns and chemical properties.


RESULTS
It was observed that cell type and surface topography are more influential than surface chemistry in determining the alignment tendency of a cell on a substrate surface. Low walls (several microns high) could not confine cells into the microgrooves of the films but alignment was still possible if the cells had a natural alignment property.


CONCLUSIONS
This information is very useful in designing tissue engineering scaffolds and in the long-term success of implants.
Middle East Technical University, Biotechnology Research Unit, Ankara 06531, Turkey. vhasirci@metu.edu.tr
Vasif
V
Hasirci
Brian J
BJ
Pepe-Mooney
D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D045744:Cell Line, Tumor; D060527:Corneal Keratocytes / Q000166:cytology; D042783:Endothelial Cells / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D055213:Retinal Pigment Epithelium / Q000166:cytology; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials