[Detection of sentinel lymph node of rabbit thyroid by fluorescence imaging].
OBJECTIVE
To detect the sentinel lymph nodes (SLN) of rabbit thyroid by fluorescence imaging and to provide experimental evidence for its clinical application.


METHODS
Equal concentration and dose (35 nmol/L, 1 ml) of ICG (indocyanine green) or ICG:CSA (calf serum albumin) complex was injected into rabbit thyroid to measure the their fluorescence amount. Twenty rabbits were randomly divided into ICG group and ICG:CSA group, with 10 rabbits each group, rabbit thyroid was separately injected with 0.02 ml of 100 nmol/L ICG and 0.02 ml of methylene, or 0.02 ml of 35 nmol/L ICG:CSA and 0.02 ml of methylene, and then ICG fluorescence and methylene staining in SLN were detected respectively.


RESULTS
Fluorescence amount of ICG:CAS was three times that of ICG with the same concentration and dose. SLNs were showed in all rabbits injected with ICG or ICG:CSA, but found only in 16 of 20 rabbits injected with methylene blue. The accuracy of fluorescence imaging and blue dye imaging were respectively 95.8% and 79.2%.


CONCLUSIONS
Fluorescence imaging, using ICG or ICG:CSA as a tracer, could be an alternative method for the detection of SLN of thyroid in future clinical practice.
Department of Otorhinolaryngology Head and Neck Surgery, Renji Hospital of Shanghai Jiaotong University School of Medicine, Shanghai 200001, China.
Zhen
Z
LIU
Jia-dong
JD
WANG
Zheng
Z
ZHOU
D000818:Animals; D005453:Fluorescence; D007208:Indocyanine Green; D008198:Lymph Nodes; D061848:Optical Imaging / Q000379:methods*; D011817:Rabbits; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*; D013961:Thyroid Gland
D007208:Indocyanine Green