[Function analysis of Sj34.9 gene based on RNAi and microarray].
OBJECTIVE
To analyze the function of Sj34.9 gene, so as to provide the reference for future studies.


METHODS
The Sj34.9 gene was knocked down in Schistosoma japonicum by RNA interference (RNAi), and the microarray was used to analyze the genes'expression of S. japonicum after Sj34.9 knocked down.


RESULTS
A total of 378 genes expressed differently including 202 up-regulated genes and 176 down-regulated genes. The pathway analysis indicated that the genes expressed differently were mainly related to organelles, metabolism and signal transduction. The gene ontology category analysis showed that most of these genes might be involved in binding, membrane fomulation and cellular process.


CONCLUSIONS
The gene Sj34.9 might play important roles in the process of growth, development, reproduction and metabolism of S. japonicum.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai 200241, China.
Ping-Ping
PP
Liu
Yan-Li
YL
Shi
Yun-Xia
YX
Yang
Jin-Ming
JM
Liu
Jiao-Jiao
JJ
Lin
Ya-Mei
YM
Jin
D000818:Animals; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020868:Gene Silencing; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012549:Schistosoma japonicum / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
D015801:Helminth Proteins