Characterization and immunomodulatory activities of sulfated polysaccharides from Capsosiphon fulvescens.
Sulfated polysaccharides isolated from Capsosiphon fulvescens and fractionated using ion-exchange chromatography were investigated to determine their chemical and molecular characteristics and biological activities. The crude and fractionated polysaccharides (F(1), F(2), and F(3)) consisted mostly of carbohydrates (28.9-67.0%), uronic acids (1.6-9.2%) and sulfates (5.2-13.4%) with various amounts of proteins (2.1-53.7%). Their monosaccharide levels were significantly different including rhamnose (20.8-65.2%), xylose (13.0-37.1%) and mannose (11.6-65.1%). The polysaccharides contained one or two subfractions with molecular weights (M(w)) ranging from 401.7×10(3) to 6232×10(3) g/mol. These polysaccharides (the crude and fraction F(2)) strongly stimulated macrophage cells, RAW264.7 cell line, producing considerable amounts of NO, PGE(2) and cytokines which suggested that they could be strong immunostimulators. The main backbone of the most immunoenhancing polysaccharide (F(2)) was suggested by GC-MS and NMR to be the following: [formula, see text].
Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon 210-702, Republic of Korea.
Supatra
S
Karnjanapratum
Mehdi
M
Tabarsa
MyoungLae
M
Cho
SangGuan
S
You
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002240:Carbohydrate Sequence; D000460:Chlorophyta / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D006019:Glycolysis; D006367:HeLa Cells; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000970:Antineoplastic Agents; D007155:Immunologic Factors; D011134:Polysaccharides; D013431:Sulfates; D014867:Water