Relationship between antioxidant enzyme genotype and activity and kidney function: a case-control study.
BACKGROUND
Oxidative stress is associated with the progression of chronic kidney disease (CKD). Links between antioxidant enzyme SNPs such as superoxide dismutase (SOD) Ala16Val, glutathione peroxidase (GPx) Pro197Leu and catalase C- 262T and CKD have not been investigated. This study compared antioxidant genotypes and activities of CKD patients with population controls, and determined their relationship to kidney function.


METHODS
CKD patients (n = 230) and controls (n = 224) were screened for the GPx, SOD and catalase SNPs. Plasma and red blood cell (RBC) GPx, RBC SOD and RBC catalase activities, and estimated glomerular filtration rate (eGFR) were measured.


RESULTS
Significantly more CKD patients (n = 5) had the GPx Leu/Leu genotype compared to controls (n = 0), and had lower eGFR (p = 0.054). CKD patients had significantly lower plasma GPx and RBC catalase activities compared to controls, whereas RBC GPx and RBC SOD activities were significantly higher in CKD patients (p < 0.001).


CONCLUSIONS
CKD is associated with reduced plasma GPx and catalase activities and enhanced RBC GPx and SOD activities. Although, genotype frequencies were similar for both groups, lower eGFR was associated with the GPx Leu/ Leu genotype.
University of Tasmania, School of Human Life Sciences, Launceston, Tasmania, University of Queensland, School of Medicine, St. Lucia, Australia.
Amanda
A
Crawford
Robert G
RG
Fassett
Jeff S
JS
Coombes
Dale A
DA
Kunde
Kiran D K
KD
Ahuja
Iain K
IK
Robertson
Madeleine J
MJ
Ball
Dominic P
DP
Geraghty
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002374:Catalase / Q000235:genetics*; D005260:Female; D005838:Genotype; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D007668:Kidney / Q000201:enzymology* / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D051436:Renal Insufficiency, Chronic / Q000201:enzymology* / Q000503:physiopathology*; D013482:Superoxide Dismutase / Q000235:genetics*; D055815:Young Adult
D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase