Lesional infiltration of receptor activator of nuclear factor-κB ligand⁺ cells in experimental autoimmune neuritis rats.
Experimental autoimmune neuritis (EAN) is a T cell-mediated autoimmune demyelinating inflammatory disease of the peripheral nervous system. Receptor activator of NF-κB ligand (RANKL), a member of the tumor necrosis factor family, regulates proliferation of mature T cells. Here, we have studied the expression of RANKL in sciatic nerves of EAN rats. EAN was induced in male Lewis rats. The spatiotemporal expression of RANKL in sciatic nerves of EAN rats was investigated using immunohistochemistry. In sciatic nerves of normal rats RANKL(+) cells were rarely seen. EAN induced a significant accumulation of RANKL(+) cells in sciatic nerves and there was a significant positive correlation of the time course of RANKL(+) cell accumulations with neurological scores of EAN rats. The major cellular resources of RANKL in sciatic nerves were T cells and macrophages. The positive association of RANKL(+) cell accumulations with neurological scores of EAN rats together with the known functions of RANKL indicated that RANKL might play a role in pathologic development of EAN and need further investigation.
Department of Rehabilitation Medicine, 2nd Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People's Republic of China. yinying@cqmu.edu.cn
Ying
Y
Yin
Hermann J
HJ
Schluesener
Zhi-Yuan
ZY
Zhang
Zhiren
Z
Zhang
D000704:Analysis of Variance; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D002452:Cell Count; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D005620:Freund's Adjuvant / Q000633:toxicity; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D017628:Microglia / Q000378:metabolism / Q000473:pathology; D020556:Neutrophil Infiltration / Q000187:drug effects / Q000502:physiology*; D009842:Oligopeptides / Q000633:toxicity; D051381:Rats; D011917:Rats, Inbred Lew; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000378:metabolism*; D012584:Sciatic Nerve; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
D015703:Antigens, CD; C482581:L-prolyl-m-L-sarcolysyl-p-L-fluorophenylalanine ethyl ester; D009842:Oligopeptides; D053246:Receptor Activator of Nuclear Factor-kappa B; D005620:Freund's Adjuvant