Aberrant expression of liver microRNA in chickens infected with subgroup J avian leukosis virus.
Subgroup J avian leukosis virus (ALV-J) is an oncogenic retrovirus primarily causing myeloid leukosis (ML) in broilers. Although ALV is well under control in a few countries including the USA, poultry industry in many parts of the world continues suffering from serious economic loss due to sporadic or widespread ALV infection, especially ALV-J infection. ALV-J infection of chickens is reportedly mediated by a cellular receptor. So far, however, no genetic variant of the receptor gene that confers resistance to ALV-J has been identified. To advance our understanding on epigenetic factors that are involved in the event of ALV-J infection, we examined the expression of miRNAs in livers of 10-week-old chickens uninfected or infected with ALV-J by miRNA microarray analysis. Our data showed there were 12 miRNAs differentially expressed in liver between the uninfected and infected groups (P<0.01). Of which, the expressions of seven miRNAs (gga-mir-221, gga-mir-222, gga-mir-1456, gga-mir-1704, gga-mir-1777, gga-mir-1790, and gga-mir-2127,) were upregulated by ALV-J infection and may be involved in oncogenicity. The other five miRNAs (gga-let-7b, gga-let-7i, gga-mir-125b, gga-mir-375, and gga-mir-458) were significantly downregulated. The downregulated miRNAs may play important roles in tumor suppression. This finding paves the way for further exploration of epigenetic influence on tumorigenicity upon ALV-J infection.
College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Hongmei
H
Li
Jun
J
Ji
Qingmei
Q
Xie
Huiqin
H
Shang
Huanmin
H
Zhang
Xuegang
X
Xin
Feng
F
Chen
Baoli
B
Sun
Chunyi
C
Xue
Jingyun
J
Ma
Yingzuo
Y
Bi
D000818:Animals; D001353:Avian Leukosis / Q000473:pathology*; D001354:Avian Leukosis Virus / Q000472:pathogenicity*; D002645:Chickens; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D008099:Liver / Q000473:pathology* / Q000821:virology*; D035683:MicroRNAs / Q000096:biosynthesis*
D035683:MicroRNAs