[Diversity and antimicrobial activities of actinomycetes from pesticide-contaminated spots in Shandong Peninsula].
OBJECTIVE
In order to study diversity and find antimicrobial activities of actinomycetes from pesticide-contaminated spots in Shandong Peninsula.


METHODS
The phylogenetic analysis of 154 isolated strains was done based on 16SrDNA sequences. Antimicrobial activities of 10 non-Streptomyces strains were tested by using cylinder-plate method and hypha growth rate method.


RESULTS
Among the strains, 154 strains belonged to 7 families, 8 genera: Streptomyces (87.01%), Kocuria, Microbacterium, Nocardiopsis, Knoellia, Pseudonocardia, Micromonospora, Actinoplanes. The fermentation broths of 10 non-Streptomyces strains had inhibitory actives against all tested phytopathogenic fungi (Botrytis cinerea, Fusarium oxysporum f. sp. niveum, Gibberella zeae, Sclerotinia sclerotiorum, Colletotrichum gloesporioides) and bacteria (Staphylococcus aureus, Bacillus subtills, Bacillus cereus, Escherichai coli, Pseudomonas aeruginos) , especially Microbacterium oxydans JN853773 and Kocuria rosea JN192402 had strong inhibitory effects.


CONCLUSIONS
Abundant diversity of actinomycetes existed in pesticide contaminated spots in Shandong Peninsula. Microbacterium oxydans JN853773 and Kocuria rosea JN192402 showed high antimicrobial activities and could be further exploited.
Institute of Pesticide Science, Northwest A&F University, Yangling, Shaanxi 712100, China. xinhongmei123abc@163.com
Hongmei
H
Xin
Zhengyan
Z
Guo
Lixia
L
Fan
Zhaonong
Z
Hu
Wenjun
W
Wu
D039903:Actinobacteria / Q000145:classification* / Q000302:isolation & purification; D000890:Anti-Infective Agents / Q000494:pharmacology*; D010575:Pesticides; D010802:Phylogeny; D012988:Soil Microbiology
D000890:Anti-Infective Agents; D010575:Pesticides