[Expression and significance of estrogen receptor alpha gene polymorphisms in blood and villi of missed abortion].
OBJECTIVE
To explore the differences of estrogen receptor α (ERα) gene polymorphism in patients with missed abortion and normal pregnancy and examine the relationship between ERα gene polymorphism and missed abortion.


METHODS
A total of 100 cases of missed abortion patients and 102 cases of normal pregnant women were selected as the experimental and control groups. And 2ml blood samples and chorionic villus specimens were collected. The method of polymerase chain reaction restriction fragment length polymorphism was employed for ERα gene PvuII and XbaI polymorphism. And the data was analyzed to explore the relationship between ERα gene polymorphism and missed abortion.


RESULTS
There was statistic significance in the frequency of ERα gene PvuII enzyme cleavage allele P and p from blood and villi samples between two groups, blood (χ(2) = 5.542, P < 0.05) OR: 1.742, villi (χ(2) = 7.559, P < 0.01), OR: 1.948. Statistic significances existed in the difference of frequency for ERα gene XbαI enzyme cleavage allele X and x from blood and villi samples between two groups, blood (χ(2) = 15.205, P < 0.01), OR:2.519; villi (χ(2) = 13.750, P < 0.01), OR: 2.499. There was a positive correlation of the frequency in ERα gene PvuII and XbαI genotype from blood and villi samples in the experimental group.


CONCLUSIONS
It suggests that ERα gene polymorphism is correlated with the pathogenesis of missed abortion. Alleles P and X may be susceptibility genes.
Department of Obstetrics & Gynecology, Affiliated Hospital, Inner Mongolia Medical College, Hohhot 010059, China.
Fang
F
Liu
Mu-ge
MG
Qi
D000030:Abortion, Missed / Q000097:blood / Q000235:genetics*; D000328:Adult; D001769:Blood / Q000378:metabolism*; D016022:Case-Control Studies; D002824:Chorionic Villi / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic; D011247:Pregnancy; D055815:Young Adult
C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha