Experiments on alcocytes containing enzyme nanoparticles for reducing toxic blood concentration of ethanol.
We developed a method of introduction of alcohol dehydrogenase and aldehyde dehydrogenase into mouse and human erythrocytes. The possibility of using erythrocytes loaded with the two enzymes (alcocytes) for reducing ethanol concentration in animal blood was studied. Injection of alcocytes to mice led to accelerated decrease in ethanol concentration as soon as after 5 min and this capacity of alcocytes persisted for at least 2 h. Alcocytes prepared from fresh or preserved human blood did not survive in mice. Thus autologous alcocytes is functionally active and can be used as a protective system in acute alcohol intoxication. The developed method can be regarded as a new medical biotechnology.
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
Yu G
YG
Kaminsky
E A
EA
Kosenko
Yu G
YG
Alexandrovich
F I
FI
Ataullakhanov
D000426:Alcohol Dehydrogenase / Q000378:metabolism / Q000627:therapeutic use; D000437:Alcoholism / Q000188:drug therapy; D000444:Aldehyde Dehydrogenase / Q000378:metabolism / Q000627:therapeutic use; D000818:Animals; D001709:Biotechnology / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology*; D000431:Ethanol / Q000097:blood* / Q000378:metabolism; D006801:Humans; D051379:Mice; D053758:Nanoparticles / Q000627:therapeutic use*; D036103:Nanotechnology / Q000379:methods*
D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase