Mariniflexile jejuense sp. nov., isolated from the junction between seawater and a freshwater spring, and emended description of the genus Mariniflexile.
A gram-negative, non-spore-forming, non-flagellated, motile-by-gliding rod, designated SSK2-3(T), was isolated from the junction between seawater and a freshwater spring at Jeju island, South Korea. Strain SSK2-3(T) grew optimally at 25-30 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SSK2-3(T) clustered with type strains of species of the genus Mariniflexile, with which it exhibited 97.2-97.8 % 16S rRNA gene sequence similarity. Sequence similarity between the isolate and the other strains used in the phylogenetic analysis was <95.6 %. Strain SSK2-3(T) contained MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 1 G and C15 : 0 as the major fatty acids. The major polar lipids of strain SSK2-3(T) were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain SSK2-3(T) was 32.4 mol%. DNA-DNA relatedness between the isolate and Mariniflexile gromovii KCTC 12570(T), Mariniflexile fucanivorans DSM 18792(T) and Mariniflexile aquimaris HWR-17(T) was 19, 15 and 20 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SSK2-3(T) is separate from other members of the genus Mariniflexile. On the basis of the data presented, strain SSK2-3(T) is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile jejuense sp. nov. is proposed. The type strain is SSK2-3(T) ( = KCTC 23958(T)  = CCUG 62414(T)). An emended description of the genus Mariniflexile is given.
University of Science and Technology (UST), 217 Gajungro, Yuseong, Daejeon 305-350, Republic of Korea.|Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 306-809, Republic of Korea.
Yong-Taek
YT
Jung
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon 440-746, Republic of Korea.
Jung-Hoon
JH
Yoon
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6