Do dietary betaine and the antibiotic florfenicol influence the intestinal autochthonous bacterial community in hybrid tilapia (Oreochromis niloticus ♀ × O. aureus ♂)?
The attractant betaine and the antibiotic growth promoter florfenicol are commonly used together in Chinese fresh water aquaculture, but there is no information about the effect of these two feed additive on the intestinal autochthonous bacterial community in hybrid tilapia (Oreochromis nilotica ♀ × O. aureas ♂). Hybrid tilapia (240 fish in total; 20 fish per net cage; three cages per group) were divided into four dietary groups: control group, no betaine or florfenical addition (CK); betaine group, 0.1% betaine added (B); florfenicol group, 0.002% florfenicol added (F); and combination group, 0.1% betaine and 0.002% florfenicol added together (BF). After 8 weeks of feeding, six fish from each cage were chosen randomly, the guts were sampled and pooled, and their intestinal autochthonous bacterial communities were analyzed by 16S rDNA-denaturing gradient gel electrophoresis. Enumeration of total gut autochthonous bacteria was analyzed by quantitative PCR with rpoB as the endogenous control. The results showed that the fish intestinal bacteria of group B were more diverse than that of CK, and that of F and BF groups was reduced in the total numbers and limited to certain bacterial species or genera (P < 0.05). This study revealed that betaine can promote some intestinal autochthonous bacteria, and florfenicol play a depressor role. When combined together, florfenicol may overshadow the effect of betaine on the predominant intestinal bacteria of tilapia.
Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
Suxu
S
He
Zhigang
Z
Zhou
Yuchun
Y
Liu
Yanan
Y
Cao
Kun
K
Meng
Penjun
P
Shi
Bin
B
Yao
Einar
E
Ringø
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D017756:Aquaculture / Q000379:methods; D001419:Bacteria / Q000187:drug effects*; D001622:Betaine / Q000008:administration & dosage*; D044822:Biodiversity; D002678:Chimera; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D004032:Diet / Q000379:methods; D041981:Gastrointestinal Tract / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013839:Thiamphenicol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D017210:Tilapia / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D001622:Betaine; C035534:florfenicol; D013839:Thiamphenicol