Identification of Legionella rubrilucens isolated from a hot spring for foot-soaking in Niigata, Japan.
In May 2011, strain HYNE-20 (=JCM 17837) was isolated from a sample of hot spring water from a foot spa in Niigata, Japan, by a plating method using glycine vancomycin polymyxin B cycloheximide α-ketoglutarate (GVPCα) medium at 36°C for 7 d. The 16S rDNA sequences (1,469bp) of this strain (accession number: AB638719) had high (99.7%) similarity to Legionella rubrilucens, and we identified that this strain was indeed Legionella rubrilucens. When this strain was cultured on buffered charcoal yeast extract α-ketoglutarate (BCYEα) agar at 36°C for 7 d, it exhibited red autofluorescence under UV light (365 nm) . The dominant cellular fatty acids of the strain HYNE-20 were 16:1ω7c (29.9%) , and the guanine-plus-cytosine (G+C) content of DNA was 49.0 mol%. This is the first report that Legionella rubrilucens was isolated from a hot spring for foot soaking.
School of Life and Environmental Science, Azabu University, Kanagawa, Japan. furuhata@azabu-u.ac.jp
Katsunori
K
Furuhata
Akiko
A
Edagawa
Hiroshi
H
Miyamoto
Yo
Y
Morimoto
Masafumi
M
Fukuyama
D001483:Base Sequence; D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005453:Fluorescence; D006294:Health Resorts; D045482:Hot Springs / Q000382:microbiology*; D007564:Japan; D007875:Legionella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007876:Legionellosis / Q000382:microbiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S