Real-time PCR genotyping assay for canine trapped neutrophil syndrome and high frequency of the mutant allele in Border collies.
Trapped neutrophil syndrome is an autosomal recessive inherited neutropenia in Border collies. The causative mutation is a 4base pair deletion in exon 19 of the canine VPS13B gene. In this study, a real-time PCR assay was developed and a genotyping survey was carried out in Border collies in Japan. The carrier frequency was 11.1%, suggesting that the mutant allele frequency is high enough to warrant measures to control and prevent the disease.
Laboratory of Clinical Pathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima, Kagoshima 890-0065, Japan.
Keijiro
K
Mizukami
Akira
A
Yabuki
Takuji
T
Kawamichi
Hye-Sook
HS
Chang
Mohammad M
MM
Rahman
Mohammad M
MM
Uddin
Moeko
M
Kohyama
Osamu
O
Yamato
D000483:Alleles; D000818:Animals; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D020022:Genetic Predisposition to Disease; D005838:Genotype; D009154:Mutation; D009503:Neutropenia / Q000235:genetics / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*