[Using of metal-affinity chromatography for identification of alkylated rat globin].
Rat globin peptides alkylated by sulfur mustard on amino-acid residues C-126, C-93 and E-27 with MH+ 1444.62 Da, 1561.66 Da, 1676.78 Da, respectively, were concentrated using metal-affinity chromatography on Cu2+. The peptides were received by trypic digestion after in vitro incubation of rat globin with 60 microM HD. Aklylated peptide with MH+ 1444.62 Da is the most sensitive biomarker, which can be concentrated from globin trypic digest, incubated with 3 microM sulfur mustard.
Ia A
IaA
Dubrovskiĭ
V D
VD
Gladilovich
I A
IA
Krasnov
E P
EP
Podol'skaia
E A
EA
Murashko
V N
VN
Babakov
D000478:Alkylation; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D006454:Hemoglobins / Q000737:chemistry* / Q000302:isolation & purification*; D009151:Mustard Gas / Q000737:chemistry*; D051381:Rats
D006454:Hemoglobins; D009151:Mustard Gas