Comparison of serum treatments to remove nonspecific inhibitors from chicken sera for the hemagglutination inhibition test with inactivated H5N1 and H9N2 avian Influenza A virus subtypes.
The hemagglutination inhibition (HI) assay is the standard diagnostic test for detection of antibodies to avian influenza viruses. It is well known that chicken serum does not require additional serum pretreatment to remove nonspecific inhibitors (NSIs). However, NSIs were recognized in certain Korean local breeds. In the present study, various treatments were compared to remove such NSIs. Heat treatment, red blood cell adsorption, and kaolin treatment did not remove NSIs effectively, and treatment with periodate only partly eliminated the NSIs. Receptor destroying enzyme (RDE) treatment appeared to effectively remove NSIs from chicken sera, regardless of breeds. It is proposed that RDE treatment should be included in the HI tests for serological diagnosis of avian Influenza A virus.
Animal, Plant and Fisheries Quarantine and Inspection Agency, 175 Anyangro Manangu Anyangsi, Gyeonggido 430-757, Republic of Korea.
Hye-Ryoung
HR
Kim
Kyoung-Ki
KK
Lee
Yong-Kuk
YK
Kwon
Min-Su
MS
Kang
Oun-Kyung
OK
Moon
Choi-Kyu
CK
Park
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens; D004912:Erythrocytes; D006385:Hemagglutination Inhibition Tests / Q000379:methods / Q000662:veterinary*; D006358:Hot Temperature; D053124:Influenza A Virus, H5N1 Subtype / Q000302:isolation & purification*; D053127:Influenza A Virus, H9N2 Subtype / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology; D007616:Kaolin
D000914:Antibodies, Viral; D007616:Kaolin