[DNA damage response in ovarian clear cell adenocarcinoma].
OBJECTIVE
To study the relationship between ovarian clear cell adenocarcinoma and DNA damage.


METHODS
14 samples were selected from clinical ovarian cases including 3 cases with normal ovarian tissue, 6 cases with poorly differentiated ovarian tumor, 5 cases with ovarian clear cell adenocarcinoma, treated by X-ray irradiation and frozen sections respectively. DNA damage response was analyzed by immunofluorescence and Western blot.


RESULTS
Before X-ray irradiation, compared to normal ovarian tissue, a large number of endogenous damage existed in ovarian clear cell adenocarcinoma, and phosphorylation of histone family 2A variant (H2AX) was abnormally enhanced 1 hour after irradiation treatment, however, DNA repair was normal in ovarian clear cell adenocarcinoma. Phosphorylation of H2AX was dispensable for p53 binding protein 1 (53BP1) activation and couldn't be colocalized in clear-type ovarian cancer tissues.


CONCLUSIONS
The abnormal DNA damage activation implies that the network of DNA damage signaling pathway may be defective.
Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Yue-gai
YG
Jia
Yue-ming
YM
Yang
Bin
B
Zuo
Lian-di
LD
Guo
Jiang-yan
JY
Lou
D018262:Adenocarcinoma, Clear Cell / Q000235:genetics*; D004249:DNA Damage / Q000235:genetics* / Q000528:radiation effects; D004260:DNA Repair / Q000235:genetics; D005260:Female; D006657:Histones / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D000071857:Tumor Suppressor p53-Binding Protein 1
C468783:H2AX protein, human; D006657:Histones; D047908:Intracellular Signaling Peptides and Proteins; D010750:Phosphoproteins; C498301:TP53BP1 protein, human; D000071857:Tumor Suppressor p53-Binding Protein 1