Saccharothrix sp. PAL54, a new chloramphenicol-producing strain isolated from a Saharan soil.
An actinomycete strain designated PAL54, producing an antibacterial substance, was isolated from a Saharan soil in Ghardaïa, Algeria. Morphological and chemical studies indicated that this strain belonged to the genus Saccharothrix. Analysis of the 16S rDNA sequence showed a similarity level ranging between 96.9 and 99.2% within Saccharothrix species, with S. longispora DSM 43749(T), the most closely related. DNA-DNA hybridization confirmed that strain PAL54 belonged to Saccharothrix longispora. It showed very strong activity against pathogenic Gram-positive and Gram-negative bacteria responsible for nosocomial infections and resistant to multiple antibiotics. Strain PAL54 secreted the antibiotic optimally during mid-stationary and decline phases of growth. One antibacterial compound was isolated from the culture broth and purified by HPLC. The active compound was elucidated by uv-visible and NMR spectroscopy and by mass spectrometry. The results showed that this compound was a D: (-)-threo chloramphenicol. This is the first report of chloramphenicol production by a Saccharothrix species.
Laboratoire de Recherche sur les Produits Bioactifs et la Valorisation de la Biomasse, Ecole Normale Supérieure de Kouba, B.P. 92, 16 050 Vieux-Kouba, Alger, Algeria.
Adel
A
Aouiche
Nasserdine
N
Sabaou
Atika
A
Meklat
Abdelghani
A
Zitouni
Christian
C
Bijani
Florence
F
Mathieu
Ahmed
A
Lebrihi
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000352:Africa, Northern; D000462:Algeria; D002701:Chloramphenicol / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013056:Spectrophotometry, Ultraviolet
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002701:Chloramphenicol