Inhibitory effect of eriodictyol on IgE/Ag-induced type I hypersensitivity.
Mast cells are the principal effector cells involved in the allergic response, through the release of histamine. We investigated the effect of eriodictyol, derived from the painted maple and yerba santa, on mast cell degranulation and on an allergic response in an animal model. We also investigated its effect on the expression of the ceramide kinase (CERK) involved in calcium-dependent degranulation, and on ceramide activation by multiple cytokines. Eriodictyol suppressed the release of beta-hexosaminidase, a marker of degranulation, and the expression of interleukin (IL)-4 mRNA. It inhibited the expression of CERK mRNA, reduced the ceramide concentration in antigen-stimulated mast cells, and suppressed the passive cutaneous anaphylaxis (PCA) reaction in mice in a dose-dependent manner. These results suggest that eriodictyol can inhibit mast cell degranulation through inhibition of ceramide kinase, and that it might potentially serve as an anti-allergic agent.
Division of Biological Science and Technology, College of Science and Technology, Yonsei University, Wonju, Korea.
Jung-Min
JM
Yoo
Ji-Hee
JH
Kim
Sae-Jin
SJ
Park
Yeo-Jin
YJ
Kang
Tack-Joong
TJ
Kim
D031002:Acer / Q000737:chemistry; D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D015550:Cell Degranulation / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D031322:Eriodictyon / Q000737:chemistry; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006969:Hypersensitivity, Immediate / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology* / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects / Q000276:immunology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D001619:beta-N-Acetylhexosaminidases / Q000037:antagonists & inhibitors / Q000378:metabolism
D018926:Anti-Allergic Agents; D015415:Biomarkers; D016207:Cytokines; D044950:Flavanones; D012333:RNA, Messenger; D007073:Immunoglobulin E; D017853:Phosphotransferases (Alcohol Group Acceptor); C066324:ceramide kinase; D001619:beta-N-Acetylhexosaminidases; C007619:eriodictyol; D002118:Calcium