Targeted tissue proteomic analysis of human astrocytomas.
Complicating proteomic analysis of whole tissues is the obvious problem of cell heterogeneity in tissues, which often results in misleading or confusing molecular findings. Thus, the coupling of tissue microdissection for tumor cell enrichment with capillary isotachophoresis-based selective analyte concentration not only serves as a synergistic strategy to characterize low abundance proteins, but it can also be employed to conduct comparative proteomic studies of human astrocytomas. A set of fresh frozen brain biopsies were selectively microdissected to provide an enriched, high quality, and reproducible sample of tumor cells. Despite sharing many common proteins, there are significant differences in the protein expression level among different grades of astrocytomas. A large number of proteins, such as plasma membrane proteins EGFR and Erbb2, are up-regulated in glioblastoma. Besides facilitating the prioritization of follow-on biomarker selection and validation, comparative proteomics involving measurements in changes of pathways are expected to reveal the molecular relationships among different pathological grades of gliomas and potential molecular mechanisms that drive gliomagenesis.
Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, United States.
Xueping
X
Fang
Chenchen
C
Wang
Brian M
BM
Balgley
Kejia
K
Zhao
Weijie
W
Wang
Fang
F
He
Robert J
RJ
Weil
Cheng S
CS
Lee
D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D056148:Chromatography, Reverse-Phase; D016000:Cluster Analysis; D019075:Electrophoresis, Capillary; D053263:Gene Regulatory Networks; D006801:Humans; D007150:Immunohistochemistry; D061346:Laser Capture Microdissection; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009367:Neoplasm Staging; D060066:Protein Interaction Maps; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics; D046888:Tissue Array Analysis; D015854:Up-Regulation
D009363:Neoplasm Proteins; D020543:Proteome