A preliminary study on the influence of glutathione S transferase T1 (GSTT1) as a risk factor for late onset Alzheimer's disease in North Indian population.
BACKGROUND
Oxidative stress plays key role in pathogenesis of Alzheimer's disease. Glutathione S-transferases (GSTs), a family of phase-II isoenzymes, play a critical role in providing protection against electrophiles and products of oxidative stress. Among different classes of GSTs, GSTM1 (Mu) and GSTT1 (theta) are found to be genetically deleted which results in decreased expression of the concerned enzyme. This study aims at preliminary analysis of the frequency of deletion of GSTM1 and GSTT1 and their association with late-onset Alzheimer's disease.


METHODS
In this study, association of the deletion type polymorphism of GST M1 and T1 as possible risk factors for dementia of Alzheimer's type was studied in 50 patients and 100 controls. Dementia was diagnosed by Diagnostic and Statistical Manual of Mental Disorders (DSM-IV) criteria, Mini Mental State Examination (MMSE) and Clinical Dementia Rating (CDR) scale. Genotyping was done by multiplex Polymerase Chain Reaction (PCR). Associations between null genotype of either GSTM1 and GSTT1 or both with Alzheimer's disease were analyzed by Chi-Square test.


RESULTS
Deletion of GSTT1 was found significantly associated with Alzheimer's disease (χ(2)=5.08, p=0.02*).


CONCLUSIONS
The odds of Alzheimer's disease in null GSTT1 is found to be increased by 2.47 times in comparison to positive GSTT1.
Department of Physiology, University College of Medical Sciences (University of Delhi) and GTB Hospital, Delhi 110095, India.
Tandra
T
Ghosh
Md
M
Mustafa
Vivek
V
Kumar
Sudip Kumar
SK
Datta
Manjit Singh
MS
Bhatia
Sabyasachi
S
Sircar
Basu Dev
BD
Banerjee
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D005838:Genotype; D005982:Glutathione Transferase / Q000172:deficiency / Q000235:genetics*; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D011569:Psychiatric Status Rating Scales; D012307:Risk Factors
D005982:Glutathione Transferase; C413545:glutathione S-transferase T1