Biodegradation of poly(ε-caprolactone) (PCL) by a new Penicillium oxalicum strain DSYD05-1.
In this study, fungi isolated from soil were screened for their ability to form clear zones on agar plates with emulsified poly(ε-caprolactone) (PCL). The most active strain, designated as DSYD05, was identified as Penicillium oxalicum on the basis of morphological characteristics and phylogenetic analysis. Mutant DSYD05-1, obtained by ultraviolet-light mutagenesis from strain DSYD05, was more effective in PCL degradation. In liquid cultures of the mutant strain with PCL emulsion, DSYD05-1 showed the highest PCL-degrading activity after 4 days of cultivation. The products of PCL degradation were analysed by mass spectrometry; the results indicated that 6-hydroxyhexanoic acid was produced and assimilated during cultivation. The degradation of PCL film by DSYD05-1 was observed by scanning electron microscopy, and was indicative of a three-stage degradation process. The degradation of amorphous parts of the film preceded that of the crystalline center and then the peripheral crystalline regions. In addition, DSYD05-1 showed a wide range of substrate specificity, with capability to degrade PCL, poly(β-hydroxybutyrate), and poly(butylene succinate), but not poly(lactic acid), indicating that the strain could have potential for application in the treatment or recycling of bio-plastic wastes.
School of Life Sciences, Northeast Normal University, Changchun 130024, China.
Fan
F
Li
Dan
D
Yu
Xiumei
X
Lin
Dongbo
D
Liu
Hongmei
H
Xia
Shan
S
Chen
D001673:Biodegradation, Environmental; D002072:Butylene Glycols / Q000378:metabolism; D002208:Caproates / Q000032:analysis / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D006880:Hydroxy Acids / Q000032:analysis / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010407:Penicillium / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism; D000094122:Polyhydroxybutyrates
D002072:Butylene Glycols; D002208:Caproates; D004271:DNA, Fungal; D006880:Hydroxy Acids; D006885:Hydroxybutyrates; D019344:Lactic Acid; D011091:Polyesters; D011108:Polymers; C089797:bionole; C016240:polycaprolactone; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C039565:6-hydroxyhexanoic acid; C033616:poly(lactide)