[Detection and application of PIS genetic deficiency gene in dairy goat].
The purpose of this study was to develop a molecular method for detecting polled intersex syndrome (PIS) genetic deficiency gene in dairy goat. Three pairs of primers, PIS-, PIS+, and NEI were designed based on PIS gene sequence (AF404302) to identify the PIS genetic deficiency genotype. For the normal phenotype, the fragments of 141 and 300 bp were obtained for the genotype PIS-PIS-, and 141, 449, and 300 bp for the genotype PIS-PIS+. For the PIS goat with the genotype PIS+PIS+, 449 and 300 bp were obtained. Two hundred and twenty-four dairy goats in one population were tested based on this method. The results showed that there were 150 PIS-PIS+, 70 PIS -PIS-, and 4 PIS+PIS+. The genotype frequency of PIS-PIS+ was 66.9%, and the gene frequency of PIS+ was 35.3% in the population. Therefore, the frequency of PIS offspring was over 12%. This study developed a method to detect PIS genetic deficiency dairy goat. The method could identify buck genotype accurately to avoid the occurrence of PIS genetic deficiency. The ease and accuracy show a strong potential of the method for use in marker assisted selection of dairy goats and healthy development of dairy goat industry.
College of AnimalScience, Inner Mongolia Agricultural University, Hohhot 010018, China. yangbo061114515@126.com
Bo
B
Yang
Li-Li
LL
Jia
De-Chao
DC
Zhao
Li-Yun
LY
Meng
Xue-Feng
XF
Liu
Yan-Jun
YJ
Zhang
Wen-Guang
WG
Zhang
Jin-Quan
JQ
Li
D000818:Animals; D012734:Disorders of Sex Development / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006041:Goats / Q000235:genetics*; D008297:Male; D010375:Pedigree; D010641:Phenotype; D055748:SOXB1 Transcription Factors / Q000235:genetics; D013577:Syndrome
D055748:SOXB1 Transcription Factors