Use of bacteriophage for biological control of Salmonella Enteritidis infection in chicken.
Bacteriophage ΦCJ07 with broad host ranges for Salmonella strains isolated from sewage effluent were used to reduce Salmonella Enteritidis (SE) infection in chickens. One-day-old chicks challenged with 5×10(7) colony-forming units/bird of SE were cohabitated with contact chicks and treated with three concentrations (10(5), 10(7) and 10(9) plaque forming units (PFU)/g) of bacteriophage prepared as a feed additive for 21days after challenge. Salmonella in the intestine was quantified and environmental contamination level was examined at 1, 2 and 3weeks after challenge. All treatments reduced intestinal SE colonization in challenged and contact chickens and reduced the environmental contamination level, but the reductions produced by 10(7) and 10(9)PFU/g of bacteriophage were significant (P<0.05) as compared with untreated controls. In addition, seven out of 10 (70%) contact chickens treated with 10(9)PFU/g of bacteriophage had no detectable intestinal Salmonella at 3weeks after treatment, suggesting that bacteriophage therapy significantly prevented the horizontal transmission of SE. These results provide important insights into preventive and control strategies against SE infection in poultry and indicate that the use of bacteriophage could reduce the incidence of Salmonella food poisoning.
Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 Hwayangdong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Tae-Hyun
TH
Lim
Myung-Seob
MS
Kim
Dong-Hun
DH
Lee
Yu-Na
YN
Lee
Jae-Keun
JK
Park
Ha-Na
HN
Youn
Hyun-Jeong
HJ
Lee
Si-Yong
SY
Yang
Young-Wook
YW
Cho
Joong-Bok
JB
Lee
Seung-Yong
SY
Park
In-Soo
IS
Choi
Chang-Seon
CS
Song
D000818:Animals; D001435:Bacteriophages / Q000502:physiology*; D002645:Chickens; D010572:Pest Control, Biological; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000821:virology*