In vitro adhesion of Candida glabrata to denture base acrylic resin modified by glow-discharge plasma treatment.
This study evaluated the potential of plasma treatments to modify the surface chemistry and hydrophobicity of a denture base acrylic resin to reduce the Candida glabrata adhesion. Specimens (n = 54) with smooth surfaces were made and divided into three groups (n = 18): control - non-treated; experimental groups - submitted to plasma treatment (Ar/50 W; AAt/130 W). The effects of these treatments on chemical composition and surface topography of the acrylic resin were evaluated. Surface free energy measurements (SFE) were performed after the treatments and after 48 h of immersion in water. For each group, half (n = 9) of the specimens were preconditionated with saliva before the adhesion assay. The number of adhered C. glabrata was evaluated by cell counting after crystal violet staining. The Ar/50 W and AAt/130 W treatments altered the chemistry composition, hydrophobicity and topography of acrylic surface. The Ar/50 W group showed significantly lower C. glabrata adherence than the control group, in the absence of saliva. After preconditioning with saliva, C. glabrata adherence in experimental and control groups did not differ significantly. There were significant changes in the SFE after immersion in water. The results demonstrated that Ar/50 W treated surfaces have potential for reducing C. glabrata adhesion to denture base resins and deserve further investigation, especially to tailor the parameters to prolong the increased wettability.
Araraquara Dental School, UNESP-University Estadual Paulista, Department of Dental Materials and Prosthodontics, Araraquara, São Paulo, Brazil.
Camila Andrade
CA
Zamperini
Haline de Lima
Hde L
Carneiro
Elidiane Cipriano
EC
Rangel
Nilson Cristino
NC
Cruz
Carlos Eduardo
CE
Vergani
Ana Lucia
AL
Machado
D000180:Acrylic Resins / Q000737:chemistry*; D000328:Adult; D001128:Argon / Q000737:chemistry*; D041221:Candida glabrata / Q000502:physiology*; D003819:Denture Bases / Q000382:microbiology*; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D013282:Stomatitis, Denture / Q000382:microbiology / Q000517:prevention & control*
D000180:Acrylic Resins; D001128:Argon