Detection of carbapenemases in Enterobacteriaceae by a commercial multiplex PCR.
A commercial multiplex PCR (hyplex SuperBug ID) was tested with a collection of 132 clinical Enterobacteriaceae strains producing different carbapenemases. The sensitivity for the detection of KPC-, VIM-, NDM-, and OXA-48-encoding genes was 100%, whereas two IMP variants were missed.
Department of Medical Microbiology, Ruhr University Bochum, Bochum, Germany. martin.kaase@rub.de
Martin
M
Kaase
Florian
F
Szabados
Lars
L
Wassill
Sören G
SG
Gatermann
D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D004755:Enterobacteriaceae / Q000201:enzymology*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; D001618:beta-Lactamases; C063912:carbapenemase