Evaluation of human papilloma virus infection in patients with esophageal squamous cell carcinoma from the Caspian Sea area, north of Iran.
BACKGROUND
HPV has been found repeatedly in esophageal squamous cell carcinoma (ESCC) tissues. However, reported detection rates of HPV DNA in these tumors have varied markedly. Differences in detection methods, sample types, and geographic regions of sample origin have been suggested as potential causes of variation. We have reported that infection of HPV DNA in ESCC tumors depends on anatomical sites of esophagus of the patients from Mazandaran, north of Iran.


METHODS
HPV DNA was examined in 46 upper, 69 middle and 62 lower third anatomical sites of esophageal squamous cell carcinoma specimens collected from Mazandaran province in north Iran, near the Caspian Littoral as a region with high incidence of ESCC. HPV L1 DNA was detected using Qualitative Real time PCR and MY09/MY11 primers.


RESULTS
28.3% of upper, 29% of middle and 25.8% of lower third of ESCC samples were positive for HPV DNA. 13.6% for males and 14.1% for females were HPV positive in all samples.


CONCLUSIONS
HPV infection is about one third of ESCC in this area. Findings in this study increase the possibility that HPV is involved in esophageal carcinogenesis. Further investigation with a larger sample size is necessary.
Department of Virology and Antimicrobial Resistant Research Center Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Yousef
Y
Yahyapour
Mahmoud
M
Shamsi-Shahrabadi
Mahmoud
M
Mahmoudi
Sepideh
S
Siadati
Shefaei Shahryar
SS
Shahryar
Javad
J
Shokri-Shirvani
Hamid
H
Mollaei
Seyed Hamid Reza
SH
Monavari
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D016009:Chi-Square Distribution; D004279:DNA, Viral; D004938:Esophageal Neoplasms / Q000473:pathology / Q000821:virology*; D004947:Esophagus / Q000473:pathology*; D005260:Female; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000150:complications*
D004279:DNA, Viral