[Study on influence of glucoside Tripterygium total tablets on metabolism in rats by NMR metabonomic technique].
OBJECTIVE
To investigate the toxic effects of Glucoside Tripterygium total on rats with nuclear magnetic resonance (NMR)-based metabonomic method.


METHODS
The influence of intragastric administration of Glucoside Tripterygium total suspension at two different doses on endogenetic metabolites in normal rat urine was determined with bio-NMR method then analyzed by pattern recognition technique and partial least-squares discriminant analysis (PLS-DA). Histopathological analysis was carried out.


RESULTS
Escalations of concentrations of urinary taurine, TMAO and glucose as well as reductions of concentrations of urinary citrate and 2-oxoglutarate were found by analysis of the 1H-NMR spectra, which was coincident with the result of histopathological analysis. The result of pathological examination indicated that pathologic change was not observed in nephridial tissue, but there were obvious changes in hepatic tissue.


CONCLUSIONS
The urinary metabomic spectra were closely associated with the hepatic toxicity, which manifested the mitochondrial dysfunctions, the abnormal energy metabolism in TCA cycle as well as the abnormal glucose metabolism.
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China. sumengxiang@cpu.edu.cn
Mengxiang
M
Su
Xuan
X
Gao
Min
M
Song
Taijun
T
Hang
Wenbin
W
Shen
Zhe
Z
Song
D000818:Animals; D019343:Citric Acid / Q000652:urine; D004750:Enteral Nutrition; D005947:Glucose / Q000378:metabolism; D005960:Glucosides / Q000008:administration & dosage; D007656:Ketoglutaric Acids / Q000652:urine; D016018:Least-Squares Analysis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics; D008744:Methylamines / Q000652:urine; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D013607:Tablets / Q000008:administration & dosage; D013654:Taurine / Q000652:urine; D029757:Tripterygium / Q000737:chemistry*
D005960:Glucosides; D007656:Ketoglutaric Acids; D008744:Methylamines; D010936:Plant Extracts; D013607:Tablets; D013654:Taurine; D019343:Citric Acid; C005855:trimethyloxamine; D005947:Glucose