Antioxidant capacity, antimicrobial activity and triterpenes isolated from Jatropha isabellei Müll Arg.
Jatropha isabellei Müll Arg. belongs to the family Euphorbiaceae. The antioxidant capacity of this plant was evaluated by the DPPH method and inhibition of thiobarbituric acid reactive substances (TBARS). Ethyl acetate fraction demonstrated the best antioxidant capacity against the DPPH free radical and its profile was similar to ascorbic acid. This fraction also presented the best results of phenolics, flavonoids and condensed tannins, showing a positive relation between the phenolic content and antioxidant activity. All fractions and the crude extract were able to inhibit significantly the TBARS production induced by Fe(II) for the two tested concentrations, reaching the basal levels. In this test, ethyl acetate and dichloromethane fraction showed good values for inhibitory concentration. The dichloromethane fraction showed the best results against the fungi Sporothrix schenckii and Fusarium proliferatum and the bacterium of Micrococcus genus. This fraction allowed the isolation of acetil aleuritolic acid and a mixture of sitosterol 3-O-β-D-glucoside and stigmasterol, described for the first time in this species.
Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Build 26, Santa Maria, CEP 97105-900, Brazil. janafrohlich@yahoo.com.br
Janaina Kieling
JK
Fröhlich
Amanda Luana Forbrig
AL
Froeder
Vanessa
V
Janovik
Tarcieli Pozzebon
TP
Venturini
Romaiana Picada
RP
Pereira
Aline Augusti
AA
Boligon
Thiele Faccim
TF
de Brum
Sydney Hartz
SH
Alves
João Batista Teixeira
JB
da Rocha
Margareth Linde
ML
Athayde
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D005670:Fusarium / Q000187:drug effects; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D031286:Jatropha / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D017208:Rats, Wistar; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification; D013175:Sporothrix / Q000187:drug effects; D013265:Stigmasterol / Q000302:isolation & purification; D013634:Tannins / Q000032:analysis; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014315:Triterpenes / Q000302:isolation & purification
D000890:Anti-Infective Agents; D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D012855:Sitosterols; D013634:Tannins; D017392:Thiobarbituric Acid Reactive Substances; D014315:Triterpenes; C472604:sitosterol-3-O-glucopyranoside; C014947:acetylaleuritolic acid; D013265:Stigmasterol