Quantitation of pyrrole-imidazole polyamide in rat plasma by high-performance liquid chromatography coupled with UV detection.
A simple and robust method using high-performance liquid chromatography with UV detection was developed and validated for the determination of six pyrrole-imidazole (PI) polyamides (HN.49, TGF-β1f, TGF-β1t, HN.50f, HN.50t, and LOX-1) in rat plasma. After the plasma proteins were precipitated with methanol containing phenacetin as an internal standard, the analytes were separated on a Luna C18 (2) (5 μm, 4.6 × 150 mm). Calibration curves were linear over the range of 0.5 to 200 μg/mL for HN.49, 0.25 to 200 μg/mL for TGF-β1f, TGF-β1t, HN.50t, and LOX-1, 1 to 200 μg/mL for HN.50f in rat plasma. The inter- and intraday precision were below 15%, and the accuracy was within 15% at the quality controls. The validated method was successfully applied to sample analysis for the pharmacokinetic study.
Department of Clinical Pharmacokinetics, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Chiba, Funabashi City 274-8555, Japan.
Tomonori
T
Kamei
Takahiko
T
Aoyama
Chihiro
C
Tanaka
Takafumi
T
Nagashima
Yukio
Y
Aoyama
Hiroyuki
H
Hayashi
Hiroki
H
Nagase
Takahiro
T
Ueno
Noboru
N
Fukuda
Yoshiaki
Y
Matsumoto
D000577:Amides / Q000097:blood* / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D007093:Imidazoles / Q000097:blood* / Q000737:chemistry; D008297:Male; D000432:Methanol / Q000737:chemistry; D010615:Phenacetin / Q000737:chemistry; D011758:Pyrroles / Q000097:blood* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D051127:Scavenger Receptors, Class E / Q000235:genetics; D013056:Spectrophotometry, Ultraviolet; D016212:Transforming Growth Factor beta / Q000235:genetics
D000577:Amides; D007093:Imidazoles; C495136:OLR1 protein, rat; D011758:Pyrroles; D051127:Scavenger Receptors, Class E; D016212:Transforming Growth Factor beta; D010615:Phenacetin; D000432:Methanol