Determination of estradiol valerate in pharmaceutical preparations and human serum by flow injection chemiluminescence.
A novel method for the detection of trace estradiol valerate (EV) in pharmaceutical preparations and human serum was developed by inhibition of luminol chemiluminescence (CL) by estradiol valerate on the zinc deuteroporphyrin (ZnDP)-enhanced luminol-K3 Fe(CN)6 chemiluminescence system. Under optimized experimental conditions, CL intensity and concentration of estradiol valerate had a good linear relationship in the ranges of 8.0 × 10(-8) to 1.0 × 10(-5) g/mL. Detection limit (3σ) was estimated to be 3.5 × 10(-8) g/mL. The proposed method was applied successfully for the determination of estradiol valerate in pharmaceutical preparations and human serum and recoveries were 97.0-105.0% and 95.5-106.0%, respectively. The possible mechanism of the CL system is discussed.
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
Wenwen
W
Liu
Liangxiao
L
Xie
Hongshuang
H
Liu
Shichao
S
Xu
Bingcheng
B
Hu
Wei
W
Cao
D004958:Estradiol / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis*
D004364:Pharmaceutical Preparations; D004958:Estradiol; D008165:Luminol