Cross-reactivity of chicken anti-Japanese encephalitis virus serum and anti-West Nile virus serum in serological diagnosis.
The cross-reactivity of Japanese encephalitis virus (JEV)-immunized chicken sera and West Nile virus (WNV)-immunized chicken sera in serological tests, such as the IgG indirect ELISA (IgG-ELISA), hemagglutination inhibition test (HI) and plaque reduction neutralization test (PRNT), for JEV and WNV were examined. The mean JEV/WNV ELISA ratio in IgG-ELISA of JEV-immunized sera was significantly higher than that of WNV-immunized sera (P<0.01). JEV-immunized chicken sera did not cross-react in the WNV HI. However, all the WNV-immunized chicken sera cross-reacted in the JEV HI. JEV-immunized chicken sera did not show the WNV neutralization titer at 90% plaque reduction, and WNV-immunized chicken sera did not showed the JEV neutralization titer at 90% plaque reduction. Therefore, it is possible that chicken JEV serum can be distinguished from WNV serum by comparing the titers of IgG-ELISA, HI or PRNT respectively.
National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan.
Jiro
J
Hirota
Shinya
S
Shimizu
Tomoyuki
T
Shibahara
Sota
S
Kobayashi
D000818:Animals; D002645:Chickens; D003429:Cross Reactions / Q000276:immunology; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007106:Immune Sera / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D009500:Neutralization Tests / Q000662:veterinary; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology; D012698:Serologic Tests / Q000662:veterinary; D014901:West Nile Fever / Q000175:diagnosis / Q000662:veterinary*; D014902:West Nile virus / Q000276:immunology*
D007106:Immune Sera; D007074:Immunoglobulin G