SJSZ glycoprotein (38 kDa) modulates expression of IL-2, IL-12, and IFN-γ in cyclophosphamide-induced Balb/c.
OBJECTIVE
Cyclophosphamide (CTX) often results in immunosuppression and cytotoxic effects. The object of this study was to understand whether Styrax japonica Siebold et al. Zuccarini (SJSZ) glycoprotein prevents immunosuppression in CTX-induced Balb/c.


METHODS
The mice were injected intraperitoneally with CTX (80 mg/kg, BW) for 1 week in the presence or absence of the SJSZ glycoprotein, and divided into five groups. Weights of the spleen and thymus, phagocytic macrophages, proliferation of splenocytes and thymocytes ([(3)H]-thymidine incorporation and expression of PCNA), natural killer (NK) cytotoxicity [MTT assay, perforin, and granzyme B], and cytokines [interleukin (IL)-2, IL-12, and interferon (IFN)-γ] were evaluated using radioactivity, biochemical reactions, immunoblot analysis, and qRT-PCR. Activity of antioxidant enzymes [superoxide dismutase (SOD), glutathione dismutase (GPx) and catalase (CAT)] was also assessed.


RESULTS
The results revealed that while the parameters assessed decreased with treatment with CTX alone, SJSZ glycoprotein (10 mg/kg, BW) in the presence of CTX significantly normalized the weights of spleen and thymus, the phagocytic effect of peritoneal macrophages, the activity of antioxidant enzymes, proliferation (splenocytes and thymocytes), NK cell cytotoxicity, and expression of IL-2, IL-12, and IFN-γ.


CONCLUSIONS
SJSZ glycoprotein can normalize activity of anti-oxidative enzymes and immune-related factors.
Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, 300 Yongbong-Dong, Gwang-ju 500-757, South Korea.
Jin
J
Lee
Kye-Taek
KT
Lim
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D003520:Cyclophosphamide; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006023:Glycoproteins / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D007165:Immunosuppression Therapy; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects; D010940:Plant Proteins / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D032362:Styrax; D013482:Superoxide Dismutase / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
D016207:Cytokines; D006023:Glycoproteins; D007155:Immunologic Factors; D010940:Plant Proteins; D018809:Proliferating Cell Nuclear Antigen; D003520:Cyclophosphamide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase