Detection of cucumber mosaic virus isolates from banana by one-step reverse transcription loop-mediated isothermal amplification.
Cucumber mosaic virus (CMV) is one of the most devastating threats to the banana industry. A single-tube, one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of CMV-infected banana and plantain (Musa spp.). The reaction was performed in a single tube at 63 °C for 90 min using a real-time turbidimeter, with an improved closed-tube visual detection system in which fluorescent dye was added to the inside of the lid prior to amplification. This RT-LAMP assay is an alternative method for the rapid detection of CMV in banana plants and tissue culture materials.
State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China.
Jun
J
Peng
Minjing
M
Shi
Zihao
Z
Xia
Junsheng
J
Huang
Zaifeng
Z
Fan
D017799:Cucumovirus / Q000235:genetics / Q000302:isolation & purification*; D005453:Fluorescence; D028521:Musa / Q000821:virology*; D009391:Nephelometry and Turbidimetry / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D013696:Temperature; D013997:Time Factors; D014773:Virology / Q000379:methods*
D012367:RNA, Viral