SLC45A3-ELK4 chimera in prostate cancer: spotlight on cis-splicing.
Using a series of detailed experiments, Zhang and colleagues establish that the prostate cancer RNA chimera SLC45A3-ELK4 is generated by cis-splicing between the 2 adjacent genes and does not involve DNA rearrangements or trans-splicing. The chimera expression is induced by androgen treatment likely by overcoming the read-through block imposed by the intergenic CCCTC insulators bound by CCCTC-binding factor repressor protein. The chimeric transcript, but not wild-type ELK4, is shown to augment prostate cancer cell proliferation.
Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
Chandan
C
Kumar-Sinha
Shanker
S
Kalyana-Sundaram
Arul M
AM
Chinnaiyan
D000818:Animals; D049109:Cell Proliferation; D006801:Humans; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics*; D009004:Monosaccharide Transport Proteins; D011471:Prostatic Neoplasms / Q000235:genetics*; D012326:RNA Splicing; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D050808:ets-Domain Protein Elk-4 / Q000235:genetics*
C494193:ELK4 protein, human; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; C551031:SLC45a3 protein, human; D050808:ets-Domain Protein Elk-4