Label-free detection of micro-RNA hybridization using surface-enhanced Raman spectroscopy and least-squares analysis.
Label-free surface-enhanced Raman spectroscopy (SERS) detection of nucleic acid hybridization is impeded by poor spectral reproducibility and the fact that the chemical signatures of hybridized and unhybridized sequences are highly similar. To overcome these issues, highly reproducible silver nanorod SERS substrates along with a straightforward least-squares (LS) technique have been employed for the quantitative determination of the relative ratios of the four nucleotide components A, C, G, and T/U before and after hybridization using a clinically relevant micro-RNA sequence.
Department of Biological and Agricultural Engineering, University of Georgia , Athens, Georgia 30602, United States.
Justin L
JL
Abell
Jeonifer M
JM
Garren
Jeremy D
JD
Driskell
Ralph A
RA
Tripp
Yiping
Y
Zhao
D016018:Least-Squares Analysis; D035683:MicroRNAs / Q000737:chemistry*; D043942:Nanotubes; D009693:Nucleic Acid Hybridization; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*
D035683:MicroRNAs; D012834:Silver