Astrogliogenesis in heterotopic allotransplants of rat embryonic neocortex.
We studied gliogenesis in transplants of rat embryonic neocortex (E14-15) in 3, 7, 15, and 30 days and 12-13 months after transplantation into the sciatic nerve of adult animals. Immunogistochemical reactions to intermediate filament proteins nestin, vimentin, glial fibrillary acidic protein were used. In transplants, vimentin- and nestin-positive precursor cells differentiate into astrocytes earlier that in the developing rat neocortex (in situ). One year after transplantation, some astrocytes start to express nestin and vimentin, which attests to the development of reactive gliosis in the transplants.
Laboratory of Functional Morphology and Central and Peripheral Nervous System, Department of General and Special Morphology, Institute of Experimental Medicine, NorthWestern Branch of the Russian Academy of Medical Sciences, St. Petersburg, Russia. lemmorphol@yandex.ru
E S
ES
Petrova
D000367:Age Factors; D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D005911:Gliosis / Q000473:pathology*; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D019579:Neocortex / Q000196:embryology / Q000637:transplantation*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D064231:Nestin; D058953:Neural Stem Cells / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000166:cytology* / Q000502:physiology; D016042:Transplantation, Heterotopic; D014184:Transplantation, Homologous; D014746:Vimentin / Q000096:biosynthesis
D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D014746:Vimentin