[Researches on relationship between genetic differentiation and chemical variation of Cinnamomum migao].
OBJECTIVE
To study the relationship between the genetic diversity and chemical variation of Cinnamomum migao.


METHODS
ISSR marker technique was used to research the genetic structure of 9 population, GC-MS was used to analyze the main ingredients of the volatile oil in C. migao.


RESULTS
The analysis on the main ingredients of the volatile oil showed that there were significant or extremely significant differences in 9 populations. The minimum variation index of population was Yunnan Funing and the maximum variation index of population was Guangxi Yueye. ISSR marker analysis showed that the average of polymorphic loci percentage (P) was 42.41%, expected heterozygosity (H) was 0.181 0, Shannon's information index (I) was 0.293 8, the Nei's genetic diversity (H(s)) in the group was 0.188 9, genetic differentiation index (G(st)) was 2.269 1. The relationship between the genetic diversity and chemical variation showed that there was no significant correlation between the main ingredients of the volatile oil and 4 indexes of genetic structure of C. migao.


CONCLUSIONS
The genetic diversity of C. migao was relatively high at the population levels, while it is low within the population levels, the relationship between chemical variation and genetic diversity was not obvious, that may indicate that other factors causes the chemical variation of C. migao.
Institute of Chinese Material Medicinal, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Meilan
M
Chen
Tao
T
Zhou
Weike
W
Jiang
Yanlei
Y
Jin
Zhannan
Z
Yang
D002681:China; D027424:Cinnamomum / Q000737:chemistry* / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D005819:Genetic Markers; D014644:Genetic Variation / Q000235:genetics*; D009822:Oils, Volatile / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D010938:Plant Oils / Q000737:chemistry*; D011110:Polymorphism, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D005819:Genetic Markers; D009822:Oils, Volatile; D010938:Plant Oils; D004247:DNA