Quantum dots for labeling live cells.
Quantum dots (QDs) are semiconductor nanocrystals with unique photo-physical properties. QDs are brightly fluorescent and photostable, and therefore represent a novel class of fluorescence probes for bio-imaging and cell monitoring. There are different techniques for labeling cells with QDs. Moreover, there is evidence that optical properties of the QD can be changed as a result of interaction with live cells. This manuscript describes a simple method for labeling of rat endothelial progenitor cells using ODs and for characterization of their optical properties.
Wallenberg Laboratory, Department of Molecular and Clinical Medicine/Clinical Physiology, The Sahlgrenska Academy and University Hospital, University of Gothenburg, Gothenburg, Sweden. yun.chen@wlab.gu.se
Yun
Y
Chen
D000818:Animals; D018929:Cell Culture Techniques; D058948:Cell Tracking / Q000379:methods*; D042783:Endothelial Cells; D005434:Flow Cytometry; D018613:Microscopy, Confocal; D045663:Quantum Dots; D051381:Rats; D013234:Stem Cells