[Diabetes mellitus ulcers treatment with Bletilla striata polysaccharide].
The aim of this study was to evaluate the efficacy of Bletilla striata polysaccharide on diabetes mellitus ulcers. Diabetes mellitus animal model was established by single ip injection of streptozotocin (STZ, 50 mg x kg(-1)) with the criteria of blood glucose > or = 16.7 mmol x L(-1) after 72 h. 4 weeks after STZ injection, each animal received two full thickness incisional wounds (1.8 cm in diameter). The wounds then were divided into B. striata polysaccharide group and PBS group. Wound closure rate, fibroblast (FB) infiltration, hydroxyproline (OHP) content and myeloperoxidase (MPO) levels were examined on day 3, 7, 14, 21 post wound. The treatment of B. striata polysaccharide significantly facilitated diabetes mellitus ulcers healing compared to PBS group. Histological analysis showed that B. striata polysaccharide markedly increased inflammatory cell infiltration in wound area. The herb also strongly evaluation of FB, OHP demonstrated a significantly increased in B. striata polysaccharide group. B. striata polysaccharide group promoted wound closure by means of enhanced inflammatory cell infiltration and re-epithelialization, and the promotion of FB and OHP levels.
Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. ylhvicky@163.com
Linhua
L
Yu
Xuqiang
X
Nie
Huijun
H
Pan
Shuang
S
Ling
Dandan
D
Zhang
Ka
K
Bian
D000818:Animals; D048909:Diabetes Complications / Q000188:drug therapy*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006909:Hydroxyproline / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage*; D011134:Polysaccharides / Q000008:administration & dosage*; D051381:Rats; D012883:Skin Ulcer / Q000188:drug therapy*; D014945:Wound Healing / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011134:Polysaccharides; D009195:Peroxidase; D006909:Hydroxyproline