Cell-penetrating peptides as delivery vehicles for a protein-targeted terbium complex.
Release after transmission: Arginine-rich, cell-penetrating peptides (CPPs) mediate cytoplasmic delivery of trimethoprim (TMP)-terbium complex conjugates and selective, intracellular labeling of E. coli dihydrofolate reductase (eDHFR) fusion proteins. A disulfide bond linking CPP and cargo is reduced following uptake. CPP conjugation can be used to deliver otherwise cell-impermeable, ligand-fluorophore conjugates.
Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA.
Shabnam
S
Mohandessi
Megha
M
Rajendran
Darren
D
Magda
Lawrence W
LW
Miller
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D002463:Cell Membrane Permeability; D057846:Cell-Penetrating Peptides / Q000737:chemistry* / Q000378:metabolism; D004285:Dogs; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D004705:Endocytosis; D004926:Escherichia coli; D005456:Fluorescent Dyes; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism; D057054:Molecular Imaging; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013194:Staining and Labeling; D013725:Terbium / Q000737:chemistry*
D057846:Cell-Penetrating Peptides; D004337:Drug Carriers; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D013725:Terbium; D001120:Arginine