Leishmanicidal active withanolides from a pakistani medicinal plant, Withania coagulans.
In the course of screening for leishmanicidal constituents from Asian and South American medicinal plants, a Pakistani medicinal plant, Withania coagulans, showed activity. We therefore studied the active components of the methanol extract of aerial parts of W. coagulans. From the ethyl acetate soluble fraction of the extract, four new withanolides (1-4) were isolated along with seven known withanolides (5-11). The new compounds were elucidated to be (14R,15R,17S,20S,22R)-14,15,17,20-tetrahydroxy-1-oxowitha-2,5,24-trienolide (1), (14R,15R,17S,20S,22R)-14,15,17,20-tetrahydroxy-1-oxowitha-3,5,24-trienolide (2), (14S,17R,20S,22R)-14,17,20-trihydroxy-1-oxowitha-2,5,24-trienolide (3), and (14S,17R,20S,22R)-14,17,20-trihydroxy-1-oxowitha-3,5,24-trienolide (4), from 1H-NMR, 13C-NMR, 2D-NMR and high resolution (HR)-MS data. Some of these compounds having the partial structure 1-oxo-2,5-diene showed strong leishmanicidal activity against Leishmania major.
Faculty of Pharmaceutical Sciemces at Kagawa Campus, Tokushima Bunri Universiyty, Shido, Sanuki, Kagawa, Japan. kuroyanagi@kph.bunri-u.ac.jp
Masanori
M
Kuroyanagi
Miki
M
Murata
Takahisa
T
Nakane
Osamu
O
Shirota
Setsuko
S
Sekita
Hiroyuki
H
Fuchino
Zabta K
ZK
Shinwari
D000981:Antiprotozoal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018320:Leishmania major / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D010154:Pakistan; D010946:Plants, Medicinal / Q000737:chemistry; D032341:Withania / Q000737:chemistry*; D054358:Withanolides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
D000981:Antiprotozoal Agents; D054358:Withanolides