Impact of the auxin signaling inhibitor p-chlorophenoxyisobutyric acid on short-term Cd-induced hydrogen peroxide production and growth response in barley root tip.
Short-term treatment (30 min) of barley roots with a low 10 μM Cd concentration induced significant H(2)O(2) production in the elongation and differentiation zone of the root tip 3h after treatment. This elevated H(2)O(2) production was accompanied by root growth inhibition and probably invoked root swelling in the elongation zone of the root tip. By contrast, a high 60 μM Cd concentration induced robust H(2)O(2) production in the elongation zone of the root tip already 1h after short-term treatment. This robust H(2)O(2) generation caused extensive cell death 6 h after short-term treatment. Similarly to low Cd concentration, exogenously applied H(2)O(2) caused marked root growth inhibition, which at lower H(2)O(2) concentration was accompanied by root swelling. The auxin signaling inhibitor p-chlorophenoxyisobutyric acid effectively inhibited 10 μM Cd-induced root growth inhibition, H(2)O(2) production and root swelling, but was ineffective in the alleviation of 60 μM Cd-induced root growth inhibition and H(2)O(2) production. Our results demonstrated that Cd-induced mild oxidative stress caused root growth inhibition, likely trough the rapid reorientation of cell growth in which a crucial role was played by IAA signaling in the root tip. Strong oxidative stress induced by high Cd concentration caused extensive cell death in the elongation zone of the root tip, resulting in the cessation of root growth or even in root death.
Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523 Bratislava, Slovak Republic. Ladislav.Tamas@savba.sk
Ladislav
L
Tamás
Beáta
B
Bočová
Jana
J
Huttová
Ľubica
Ľ
Liptáková
Igor
I
Mistrík
Katarína
K
Valentovičová
Veronika
V
Zelinová
D002104:Cadmium / Q000633:toxicity*; D016923:Cell Death / Q000187:drug effects; D002995:Clofibric Acid / Q000494:pharmacology*; D001467:Hordeum / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism*; D018519:Meristem / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors
D007210:Indoleacetic Acids; D002104:Cadmium; D002995:Clofibric Acid; D006861:Hydrogen Peroxide