Allergic immune-regulatory effects of adlay bran on an OVA-immunized mice allergic model.
Allergy is an inflammation associated with an elevated T helper (Th) 2 lymphocyte responses to allergens and elevated serum IgE levels and cytokines. In one of our previous studies using a cell model, various flavonoids were found to be involved the anti-inflammatory effects of adlay bran. The present study investigated the effect of the ethyl-acetate fraction of ethanolic extract of adlay bran (ABE-EtOAc) in an ovalbumin (OVA)-immunized murine model. Six-week-old female BALB/c mice underwent OVA sensitization and were used as an allergy model. An orogastric gavage was used to force feed these mice with 240 mg/kg ABE-EtOAc from their sixth week through their twelfth week. Immune reactions were determined by measuring changes in Th2-type cytokine (IL-4 and IL-5) levels and production of antibodies. ABE-EtOAc was found capable of regulating the Th1/Th2 immune reaction through its regulation of IL-2 and IL-4. It also significantly reduced the production of anti-OVA IgE antibodies (10%), increased the secretion of IFN-γ and decreased the secretion of IL-6 (38%). These results suggest that adlay bran extract can reduce an allergic reaction by balancing Th1/Th2 immune responses and that it might be used in the treatment of this condition.
Institute of Food Science and Technology, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan.
Hong-Jhang
HJ
Chen
Hsin-Yi
HY
Hsu
Wenchang
W
Chiang
D000821:Animal Feed; D000818:Animals; D031742:Coix / Q000737:chemistry*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation; D006967:Hypersensitivity / Q000517:prevention & control*; D007113:Immunity, Innate / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012639:Seeds; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D016207:Cytokines; D010936:Plant Extracts; D000431:Ethanol; D010047:Ovalbumin