Antidiabetic, anti-oxidant and antimicrobial activities of Fadogia ancylantha extracts from Malawi.
BACKGROUND
Communities in Chilumba, Malawi use herbal tea prepared from Fadogia ancylantha Schweinf (Rubiaceae) leaves for the management of diabetes, hypertension and alleviation of symptoms of gastrointestinal disorders and pneumonia. The objective of the study was to evaluate the in vitro antidiabetic, anti-oxidant and antimicrobial activities of the crude extracts of the leaves prepared by using three different extraction methods.


METHODS
Each of the organic, cold and hot aqueous extracts of the herbal tea was evaluated for its effect on glucose uptake in C2C12 muscle and Chang cell lines. Metformin and insulin were used as positive controls. The anti-oxidant activity, based on neutralisation of DPPH free radicals, was determined spectrophotometrically. The Agar serial dilution method was utilised to determine the minimum inhibitory concentration (MIC) of the extracts for the selected fungal and bacterial strains.


CONCLUSIONS
The organic extract (12.5μg/ml) exhibited the highest in vitro glucose uptake increases in Chang cells (181.24±0.29%) and C2C12 muscle cells (172.29±0.32%) while the hot and cold aqueous extracts gave lower uptakes, 145.94±0.37% and 138.70±0.52% in Chang cells respectively. At 100μg/ml, aqueous extracts gave significantly higher (p<0.01) anti-oxidant activity (range 85.78-86.29%) than their organic counterpart (68.16%). The minimum inhibitory concentration (156μg/ml) was obtained in the organic extract against the fungus Aspergillus fumigatus and moderate growth inhibition was observed with other test micro-organisms. The hot aqueous extract inhibited the growth of all test organisms except Pseudomonas aeruginosa. The cold aqueous extract was inactive against Pseudomonas aeruginosa and Candida albicans. The differences in the MIC values between the aqueous extracts seem to suggest that raised temperatures, as traditionally practised, facilitate the extraction of secondary bioactive metabolites.


CONCLUSIONS
These results show that Fadogia ancylantha extracts have high antidiabetic and anti-oxidant properties.
Chemistry Department, Chancellor College, University of Malawi, P.O. Box 280, Zomba, Malawi. kk_nyirenda2004@yahoo.com
K K
KK
Nyirenda
J D K
JD
Saka
D
D
Naidoo
V J
VJ
Maharaj
C J F
CJ
Muller
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects / Q000254:growth & development; D001232:Aspergillus fumigatus / Q000187:drug effects / Q000235:genetics; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D002460:Cell Line; D005947:Glucose / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008295:Malawi; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D011549:Pseudomonas / Q000187:drug effects / Q000254:growth & development; D019666:Rubiaceae
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000975:Antioxidants; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D005947:Glucose