Preparation and quality assessment of itraconazole transfersomes.
Drug-loading transfersomes were prepared with itraconazole, a lipophilic drug, as a model drug to investigate the key factor affecting transfersomes quality and to evaluate their qualities. Drug-loading transfersomes were prepared using film dispersion method. The quality of transfersomes was evaluated by HPLC, transmission electron microscope, particle size analyzer and in vitro release. Itraconazole transfersomes was transparent solution in ivory white color with a mean entrapment efficiency of about 80%. The shape of hollow vesicles was spheroidal with the diameter of approximately 100 nm, and the zeta potential of 45 mV, which had a good transdermal effect. It can be concluded via single-factor investigation that the quality of transfersomes is significantly affected by solvent, salt ion concentration and homogenization pressure and so on. The preparation method obtained through screening and optimizing formulation and technology is feasible and the quality can be controlled.
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing City Key Laboratory of Drug Delivery Technology and Novel Formulations, Beijing 100050, People's Republic of China. zhengwensheng@imm.ac.cn
Wen-sheng
WS
Zheng
Xia-qin
XQ
Fang
Lu-lu
LL
Wang
Yu-jia
YJ
Zhang
D000279:Administration, Cutaneous; D000818:Animals; D003840:Deoxycholic Acid / Q000008:administration & dosage / Q000737:chemistry*; D017964:Itraconazole / Q000008:administration & dosage / Q000737:chemistry*; D054709:Lecithins / Q000008:administration & dosage / Q000737:chemistry*; D008081:Liposomes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000033:anatomy & histology / Q000187:drug effects; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000737:chemistry*
D054709:Lecithins; D008081:Liposomes; D013501:Surface-Active Agents; D003840:Deoxycholic Acid; D017964:Itraconazole