Improvement of human skin cell growth by radiation-induced modifications of a Ge/Ch/Ha scaffold.
Gelatin-/chitosan-/hyaluronan-based biomaterials are used in tissue engineering as cell scaffolds. Three gamma radiation doses (1, 10 and 25 kGy) were applied to scaffolds for sterilization. Microstructural changes of the irradiated polymers were evaluated by using scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). A dose of 25 kGy produced a rough microstructure with a reduction of the porosity (from 99 to 96 %) and pore size (from 160 to 123 μm). Radiation also modified the glass transition temperature between 31.2 and 42.1 °C (1 and 25 kGy respectively). Human skin cells cultivated on scaffolds irradiated with 10 and 25 kGy proliferated at 48 h and secreted transforming growth factor β3 (TGF-β3). Doses of 0 kGy (non-irradiated) or 1 kGy did not stimulate TGF-β3 secretion or cell proliferation. The specific growth rate and lactate production increased proportionally to radiation dose. The use of an appropriate radiation dose improves the cell scaffold properties of biomaterials.
Centro de Biotecnología, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile. cristian.acevedo@usm.cl
Cristian A
CA
Acevedo
Rodrigo A
RA
Somoza
Caroline
C
Weinstein-Oppenheimer
Samy
S
Silva
Macarena
M
Moreno
Elizabeth
E
Sánchez
Fernando
F
Albornoz
Manuel E
ME
Young
William
W
Macnaughtan
Javier
J
Enrione
D001672:Biocompatible Materials / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D049109:Cell Proliferation / Q000528:radiation effects; D048271:Chitosan / Q000737:chemistry; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D007773:Lactates / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D012867:Skin / Q000166:cytology*; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D053782:Transforming Growth Factor beta3 / Q000378:metabolism
D001672:Biocompatible Materials; D007773:Lactates; D053782:Transforming Growth Factor beta3; D005780:Gelatin; D006820:Hyaluronic Acid; D048271:Chitosan