C-terminal heat shock protein 70 of Mycobacterium tuberculosis as a molecular adjuvant for DNA vaccination with the porcine circovirus type 2 ORF2 (capsid) gene in mice.
Mycobacterium tuberculosis heat shock protein 70 (HSP70) and the peptide binding C-terminal portion of HSP70 (amino acids 359-610; HSP70c) exert an adjuvant effect when used in vaccines. To enhance the immunogenicity of a DNA vaccine against porcine circovirus type 2 (PCV2), recombinant plasmids encoding the PCV2 ORF2 (capsid) gene fused to full length hsp70 (pCA-TCH) or truncated C-terminal hsp70c (pCA-TCHc) were constructed. Immunisation of mice with pCA-TCHc induced higher serum immunoglobulin G antibody levels, stronger T helper 1 immune responses and lower PCV2 viral titres following challenge than immunisation with pCA-TCH or Cap plasmids only.
Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences No. 427, Harbin 150001, China.
Fang
F
Fu
Huabin
H
Tian
Xuesong
X
Li
Yuekun
Y
Lang
Guangzhi
G
Tong
Siguo
S
Liu
Haizhong
H
Li
Wei
W
Wang
Xi
X
Li
Xin
X
Chen
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D036022:Capsid Proteins / Q000276:immunology*; D018173:Circoviridae Infections / Q000517:prevention & control*; D017925:Circovirus / Q000145:classification* / Q000378:metabolism*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000502:physiology; D019444:Vaccines, DNA / Q000276:immunology; D019562:Viral Load; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D036022:Capsid Proteins; D018840:HSP70 Heat-Shock Proteins; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D014765:Viral Vaccines