Environmental Scanning Electron Microscopy characterization of the adhesion of conidia from Penicillium expansum to cedar wood substrata at different pH values.
Initial microbial adhesion to surfaces is a complicated process that is affected by a number of factors. An important property of a solution that may influence adhesion is pH. The surface properties of the cedar wood were characterized by the sessile drop technique. Moreover, the interfacial free energy of surface adhesion to the cedar wood was determined under pH values (2, 3, 5, 7, 9 and 11). The results showed that cedar wood examined at different pH levels could be considered hydrophobic ranged from Giwi = -13.1 mJ/m(2) to Giwi = -75 mJ/m(2). We noted that the electron-donor character of cedar wood was important at both basic and limit acidic conditions (pH 11 and pH 3) and it decreased at intermediate pH (pH 5). The cedar wood substratum presents a weak electron acceptor under various pH's. In addition, the adhesion of conidia from Penicilllium expansum to the cedar wood surfaces at different pH values (2, 3, 5, 7, 9 and 11) was investigated using Environmental Scanning Electron Microscopy and image analysis was assessed with the Mathlab(®) program. The data analysis showed that the conidia from P. expansum were strongly influenced by the pH. The maximum adhesion occurs in the pH 11 and pH 3 and decreased to 24% at pH 5.
Equipe de recherche, Laboratoire de Biotechnologie Microbienne, Faculté des Sciences et Techniques de Fès- Saïs-Maroc, B.P. 2202, Route d'Imouzzer Fès, Maroc.
Soumya
S
El Abed
Saad Koraichi
SK
Ibnsouda
Hassan
H
Latrache
Hasna
H
Meftah
Nezha Joutey
NJ
Tahri
Fatima
F
Hamadi
D028203:Cedrus / Q000737:chemistry / Q000382:microbiology*; D002448:Cell Adhesion; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007091:Image Processing, Computer-Assisted; D008855:Microscopy, Electron, Scanning; D010407:Penicillium / Q000502:physiology*; D013172:Spores, Fungal / Q000502:physiology*; D014934:Wood / Q000737:chemistry / Q000382:microbiology