Effects of X-ray irradiation of female mice in preconceptive period on polymorphism of simple repeats in DNA of offspring of different sex.
Sibs groups of F1-offspring born by non-irradiated mice and by female mice exposed to X-ray radiation in preconceptive period (50-200 cGy) were compared. Arbitrary primed PCR revealed significantly increased polymorphism of simple DNA repeats in somatic tissues of the offspring from female mice irradiated in a dose of 200 cGy. The increase in DNA polymorphism in postmitotic brain tissues and in peripheral blood was more pronounced than in proliferating spleen tissues and in the epithelium of tail tip. In the tissues of female offspring from irradiated mothers, higher increase in DNA polymorphism was observed in comparison with the tissues of male offspring from the same mothers.
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushino, Russia.
L A
LA
Fomenko
M G
MG
Lomaeva
V G
VG
Bezlepkin
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000528:radiation effects; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004307:Dose-Response Relationship, Radiation; D004848:Epithelium / Q000378:metabolism / Q000528:radiation effects; D004973:Estrus Synchronization; D005260:Female; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D018895:Microsatellite Repeats / Q000235:genetics*; D008938:Mitosis / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000528:radiation effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000097:blood / Q000209:etiology / Q000235:genetics*; D012737:Sex Factors; D013154:Spleen / Q000378:metabolism / Q000528:radiation effects; D013623:Tail / Q000378:metabolism / Q000528:radiation effects; D014916:Whole-Body Irradiation / Q000009:adverse effects*; D014965:X-Rays / Q000009:adverse effects*
D004247:DNA