Biology of fowl adenovirus type 1 infection of heterologous cells.
The JM1/1 strain of fowl adenovirus (FAV) serotype 1 isolated from gizzard erosion was used to investigate the biology of FAV in homologous (susceptible) and heterologous cells. The FAV JM1/1 strain is capable of efficient multiplication in primary chicken kidney (CK) cells, but not in Crandell-Rees feline kidney (CRFK) cells or Vero cells. FAV adsorption in heterologous cells was slightly higher than in CK cells. An early gene encoding a DNA-binding protein and a late gene encoding the hexon protein were expressed in CK cells. Only the early gene was expressed in Vero cells. Neither of these genes was expressed in CRFK cells. These results suggest that the virus was unable to multiply effectively due to suppression of viral gene expression in the heterologous cells used in this study.
Department of Microbiology II, School of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan. tsatoshi@azabu-u.ac.jp
Satoshi
S
Taharaguchi
Rina
R
Fukazawa
Miho
M
Kitazume
Hayato
H
Harima
Kensuke
K
Taira
Kenji
K
Oonaka
Motonobu
M
Hara
D000256:Adenoviridae / Q000254:growth & development / Q000502:physiology*; D000818:Animals; D002415:Cats; D002478:Cells, Cultured; D002645:Chickens; D002522:Chlorocebus aethiops; D020869:Gene Expression Profiling; D058507:Host Specificity; D053585:Virus Attachment; D014779:Virus Replication