Golgin160 recruits the dynein motor to position the Golgi apparatus.
Membrane motility is a fundamental characteristic of all eukaryotic cells. One of the best-known examples is that of the mammalian Golgi apparatus, where constant inward movement of Golgi membranes results in its characteristic position near the centrosome. While it is clear that the minus-end-directed motor dynein is required for this process, the mechanism and regulation of dynein recruitment to Golgi membranes remains unknown. Here, we show that the Golgi protein golgin160 recruits dynein to Golgi membranes. This recruitment confers centripetal motility to membranes and is regulated by the GTPase Arf1. Further, during cell division, motor association with membranes is regulated by the dissociation of the receptor-motor complex from membranes. These results identify a cell-cycle-regulated membrane receptor for a molecular motor and suggest a mechanistic basis for achieving the dramatic changes in organelle positioning seen during cell division.
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Smita
S
Yadav
Manojkumar A
MA
Puthenveedu
Adam D
AD
Linstedt
D001324:Autoantigens / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004398:Dyneins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D000077614:Golgi Matrix Proteins; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008938:Mitosis / Q000502:physiology; D020409:Molecular Motor Proteins / Q000378:metabolism*
D001324:Autoantigens; C108388:GOLGA3 protein, human; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; D020409:Molecular Motor Proteins; D004398:Dyneins