[Synthesis of benzothiazole derivatives and their binding characteristics with beta-amyloid].
Alzheimer's disease (AD) is a progressive neurodegenerative disease endangering human health seriously. Recent reports have revealed that beta-amyloid aggregates play a key role in the pathogenesis of AD. Thus, targeting the Abeta plaques benzothiazole derivatives were synthesized with the scaffold of the most promising imaging agent PIB ([11C]-6-OH-BTA-1, [11C]-2-(4-(methylamino)phenyl)-6-hydroxybenzothiazole) and C = N as linker to study the binding characteristics with the target protein through surface plasmon resonance (SPR) technique. These derivatives were synthesized through simple yet effective method with high yields and characterized by 1H NMR and FTIR. The binding properties (K(D)) were determined with Biacore X-100 instrument according to the fitting-plot curve. Compounds 3a and 3f showed high binding affinity for Abeta1-40. The results suggest that benzothiazole derivatives could be served as a scaffold to develop novel beta-amyloid imaging agents for the diagnosis of AD.
Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China.
Lin
L
Zhou
Chang-Sheng
CS
Gan
Hao-Shu
HS
Wang
Zhen-Zhen
ZZ
Zhao
Jian
J
Pan
D000544:Alzheimer Disease / Q000175:diagnosis*; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D000814:Aniline Compounds / Q000737:chemistry; D052160:Benzothiazoles / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry*; D011485:Protein Binding; D012545:Schiff Bases / Q000138:chemical synthesis* / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013844:Thiazoles / Q000737:chemistry
C475519:2-(4'-(methylamino)phenyl)-6-hydroxybenzothiazole; D016229:Amyloid beta-Peptides; D000814:Aniline Compounds; D052160:Benzothiazoles; D010446:Peptide Fragments; D012545:Schiff Bases; D013844:Thiazoles; C078592:amyloid beta-protein (1-40)