Effect of Coriolus versicolor polysaccharides on the hematological and biochemical parameters and protection against Aeromonas hydrophila in allogynogenetic crucian carp (Carassius auratus gibelio).
The effect of dietary intake of Coriolus versicolor Polysaccharides (CVP) on the hematological and biochemical indices of Allogynogenetic crucian carp (Carassius auratus gibelio) was investigated. Fish were fed CVP supplemented diets (0, 0.25, 0.5, 1.0, 2.0 or 4.0 g CVP kg(-1)) for 56 days. The RBC, WBC counts, hemoglobin content, ESR in blood and TP, ALT, AST, ALP, GLU, CHO, TG, and BUN in serum were measured on day 0, 14, 28, 42, and 56. After feeding of 56 days, fish were infected with Aeromonas hydrophila and mortalities were recorded. The results indicated that feeding crucian carp with suitable dose of CVP enhanced the RBC, WBC counts, hemoglobin and TP content, ALP activity, and decreased the ESR, ALT, AST, GLU, CHO, TG and BUN. There was no effect in fish at low dose (0.25 g kg(-1)). Unexpectedly, the higher CVP dose used here (2.0 and 4.0 g kg(-1)) has a negative effect in fish. The results of challenge experiment indicated that a moderate level of CVP in the diet (1.0 g kg(-1)) was the most effective to enhance the survival of fish after infected with A. hydrophila. In summary, the use of CVP, as dietary supplements, can improve the innate defense of crucian carp providing resistance to pathogens.
College of Fisheries, Huazhong Agriculture University, Wuhan 430070, Hubei Province, China.
Zhi-xin
ZX
Wu
Su-feng
SF
Pang
Xiao-xuan
XX
Chen
Yan-mei
YM
Yu
Jin-min
JM
Zhou
Xi
X
Chen
Li-jiao
LJ
Pang
D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D017756:Aquaculture / Q000379:methods; D001772:Blood Cell Count / Q000662:veterinary; D001774:Blood Chemical Analysis / Q000662:veterinary; D002347:Carps; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005393:Fish Diseases / Q000382:microbiology* / Q000517:prevention & control*; D062610:Fungal Polysaccharides / Q000494:pharmacology*; D016905:Gram-Negative Bacterial Infections / Q000517:prevention & control / Q000662:veterinary*; D006454:Hemoglobins / Q000032:analysis; D055454:Trametes / Q000737:chemistry*
D062610:Fungal Polysaccharides; D006454:Hemoglobins