Protocol for the detection and genotyping of human papillomaviruses using a liquid bead microarray assay.
More than 100 human papillomavirus (HPV) types have been identified, and over 40 of them infect the anogenital epithelium. Because each HPV type is associated with different risks for the development of cervical cancer, detecting and genotyping HPVs has increasingly become an integral part of cervical cancer control. Here, we describe a Luminex assay-based liquid bead microarray assay for genotyping 37 HPV types, which is objective, scalable, amenable to a high-throughput configuration, and has the potential to be automated.
Department of Pathology, University of Washington, Seattle, WA, USA.
Stephen
S
Cherne
Viorica
V
Popov
Qinghua
Q
Feng
D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral