[Effects of electromagnetic pulse exposure on the permeability of inner blood-retinal barrier model in vitro].
OBJECTIVE
To establish the inner blood-retinal barrier (BRB) model in vitro by co-culturing RF/6A cells and C6 cells and to investigate the effects of EMP (200 kV/m, 200 pulses) exposure on the permeability of the inner BRB model in vitro.


METHODS
RF/6A cells and C6 cells were co-cultured on transwell, and the characteristic of the inner BRB model was assessed by detecting transendothelial electrical resistance (TEER) and the permeability of horseradish peroxidase (HRP). The co-cultured model was exposed or sham exposed to the EMP (200 kV/m 200 pulses) for 0.5, 3, 6, 12, 24 h in vitro, then TEER and the permeability of HRP were measured for studying the effects of EMP on the permeability of inner BRB model in vitro.


RESULTS
TEER value (145 Ωcm(2)) of the co-culturing inner BRB model significantly increased, as compared to that of RF/6A cells alone model (P < 0.05) on the 6th day after inoculation. There was significant difference of permeability of HRP between the co-culturing inner BRB model and RF/6A cells alone model (P < 0.05). The ability of inhibiting large molecular materials in the co-culturing inner BRB model enhanced. The TEER value decreased and the permeability of HRP increased as compared to the sham group at 0.5, 3, 6 h after the exposure.


CONCLUSIONS
The inner BRB model by co-culturing RF/6A cells and C6 cells in vitro is efficient and suitable to study the alterations of the restricted permeability function of the inner BRB. EMP (200 kV/m for 200 pulses) could induce the enhanced permeability of the inner BRB model in vitro.
Department of Radiation Medicine, College of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, China.
Hai-juan
HJ
Li
Long-long
LL
Yang
Wei
W
Tian
Jun-ju
JJ
Liu
Xue-jun
XJ
Xie
Guo-zhen
GZ
Guo
D000818:Animals; D001813:Blood-Retinal Barrier / Q000502:physiology*; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D017097:Electric Impedance; D004574:Electromagnetic Fields; D042783:Endothelial Cells / Q000502:physiology*; D008253:Macaca mulatta; D010539:Permeability; D051381:Rats; D012160:Retina / Q000166:cytology*