Comparative investigation of elastic properties in a trabecula using micro-Brillouin scattering and scanning acoustic microscopy.
Micro-Brillouin scattering (μ-BR) and a 200 MHz scanning acoustic microscope (SAM) with similar spatial resolutions were applied to evaluate tissue elastic properties in two directions in a trabecula. Acoustic impedance measured by SAM was in the range of 5-9 Mrayl. Wave velocities determined by μ-BR were in the range of (4.75-5.11) × 10(3) m/s. Both exhibited a similar trend of variation across the trabecula and were significantly correlated (R(2) = 0.63-0.67, p < 0.01). μ-BR is useful for the evaluation of tissue stiffness within a trabecula. Combined with SAM or nanoindentation, it can provide additional information to assess elastic anisotropy at the micro-scale.
Laboratory of Ultrasonic Electronics, Doshisha University, 1-3 Tatara-Miyakodani, Kyotanabe, Kyoto 610-0321, Japan. kawabe_m@hitachizosen.co.jp
Masahiko
M
Kawabe
Kenji
K
Fukui
Mami
M
Matsukawa
Mathilde
M
Granke
Amena
A
Saïed
Quentin
Q
Grimal
Pascal
P
Laugier
D000818:Animals; D002417:Cattle; D004548:Elasticity; D005269:Femur / Q000502:physiology*; D033401:Microscopy, Acoustic / Q000379:methods; D012542:Scattering, Radiation; D013018:Sound Spectrography