DNA methyltransferases are required to induce heterochromatic re-replication in Arabidopsis.
The relationship between epigenetic marks on chromatin and the regulation of DNA replication is poorly understood. Mutations of the H3K27 methyltransferase genes, Arabidopsis trithorax-related protein5 (ATXR5) and ATXR6, result in re-replication (repeated origin firing within the same cell cycle). Here we show that mutations that reduce DNA methylation act to suppress the re-replication phenotype of atxr5 atxr6 mutants. This suggests that DNA methylation, a mark enriched at the same heterochromatic regions that re-replicate in atxr5/6 mutants, is required for aberrant re-replication. In contrast, RNA sequencing analyses suggest that ATXR5/6 and DNA methylation cooperatively transcriptionally silence transposable elements (TEs). Hence our results suggest a complex relationship between ATXR5/6 and DNA methylation in the regulation of DNA replication and transcription of TEs.
Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.
Hume
H
Stroud
Christopher J
CJ
Hale
Suhua
S
Feng
Elena
E
Caro
Yannick
Y
Jacob
Scott D
SD
Michaels
Steven E
SE
Jacobsen
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D044127:Epigenesis, Genetic / Q000235:genetics; D015870:Gene Expression; D006570:Heterochromatin / Q000235:genetics; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics; D059765:Homologous Recombination; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D018741:Replication Origin / Q000235:genetics; D017423:Sequence Analysis, RNA
D029681:Arabidopsis Proteins; D004251:DNA Transposable Elements; D006570:Heterochromatin; C542364:ATXR5 protein, Arabidopsis; C542365:ATXR6 protein, Arabidopsis; D008780:Methyltransferases; D011495:Histone-Lysine N-Methyltransferase