[Quantitative analysis of four alkaloids in Xianglian preparations by QAMS method].
OBJECTIVE
To examine the feasibilities of the method, quantitative analysis of multi-components by single marker (QAMS) for determination of four alkaloids in Xianglian preparations.


METHODS
A HPLC method was developed to determine the contents of four alkaloids, epiberberine, coptisine, palmatine and berberine in Xianglian preparations. Berberine was selected as the internal reference substance, and 3 relative correction factors to berberine were calculated. The contents of the four alkaloids in 16 batches of samples were determined by both external standard method and QAMS. The new method was evaluated by comparing the quantitative results with of the external standard method.


RESULTS
No significant differences were found between the quantitative results of four alkaloids in 16 batches of samples determined by the two methods.


CONCLUSIONS
QAMS is feasible for determination of four alkaloids simultaneously, and the developed method can be used for quality control of Xiang Lian preparations.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. sanmijing@yahoo.com.cn
Jingjing
J
Kong
Jingjing
J
Zhu
Zhimin
Z
Wang
Qiwei
Q
Zhang
Weihong
W
Feng
D000470:Alkaloids / Q000032:analysis*; D001599:Berberine / Q000031:analogs & derivatives / Q000032:analysis / Q000592:standards*; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D039323:Coptis / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005069:Evaluation Studies as Topic; D011786:Quality Control; D012015:Reference Standards
D000470:Alkaloids; D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; C034384:coptisine; D001599:Berberine; C061432:epiberberine; C005413:palmatine