Histoenzymological characteristics of the contractile myocardium in experimental stenosis of the aorta.
Contractile cardiomyocyte metabolism was studied by histochemical methods in experimental stenosis of the aorta complicated and not by heart failure. Acceleration of the citric acid cycle, more intense oxidation of free fatty acids and their metabolites, glycolysis intensification, and higher activity of shuttle mechanisms were found in the contractile cardiomyocytes in stenosis of the aorta not complicated by heart failure. The presence of these metabolic shifts in the myocardium of all studied compartments suggests their association with not only more intense heart work, but also with the effects of total systems neurohumoral factors. Comparative study of myocardial metabolism in two variants of experimental stenosis of the aorta has revealed changes prognostically unfavorable for the development of heart failure. These changes include exhaustion of glycogen reserve, glycolysis inhibition, and metabolism shift towards biosynthetic processes. These data indicate an important role of glycolysis in support of myocardial contractile function during the acute phase of pressure overloading of the heart.
Russian State Medical University, Moscow, Russia. mtver@rsmu.ru
M S
MS
Tverskaya
V V
VV
Sukhoparova
V V
VV
Karpova
M Kh
MKh
Kadyrova
V Yu
VY
Klyuchikov
D000818:Animals; D001024:Aortic Valve Stenosis / Q000150:complications / Q000201:enzymology*; D002952:Citric Acid Cycle; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006168:Guinea Pigs; D006333:Heart Failure / Q000150:complications / Q000201:enzymology*; D006352:Heart Ventricles / Q000201:enzymology; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D009200:Myocardial Contraction; D009206:Myocardium / Q000201:enzymology* / Q000473:pathology; D009252:NADPH Dehydrogenase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D054088:Ventricular Septum / Q000201:enzymology
D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D013385:Succinate Dehydrogenase; D009252:NADPH Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase