[Immobilization of a recombinant strain producing glucose isomerase on SiO2-xerogel and properties of prepared biocatalysts].
An original method of immobilization of nongrowing microorganism cells on xerogel of silicon dioxide containing insoluble hydroxyl compounds of cobalt(III) has been developed. A recombinant strain producing glucose isomerase has been constructed on the basis of Escherichia coli with the use of a gene of Arthrobacter nicotianae. It was revealed that glucose isomerase activity and stability of biocatalysts prepared on the basis of the recombinant E. coli strain was 3-5 times greater compared with the biocatalysts prepared with the use of the donor strain A. nicotianae. Under conditions of continuous hydrolysis of 3 M fructose at 62-65 degrees C in a fixed bed reactor, time of half-inactivation of the biocatalysts prepared from the recombinant strain and A. nicotianae was -60 and -25 days, respectively.
G A
GA
Kovalenko
L V
LV
Perminova
T V
TV
Chuenko
L I
LI
Sapunova
E A
EA
Shliakhotko
A G
AG
Lobanok
D019747:Aldose-Ketose Isomerases / Q000235:genetics / Q000378:metabolism*; D001173:Arthrobacter / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D055162:Biocatalysis; D019149:Bioreactors; D018914:Cells, Immobilized; D003035:Cobalt / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry; D006358:Hot Temperature; D020100:Hydrogels; D007700:Kinetics; D060847:Metabolic Engineering / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D012822:Silicon Dioxide / Q000737:chemistry*
D001426:Bacterial Proteins; D020100:Hydrogels; D005632:Fructose; D003035:Cobalt; C025913:cobaltous nitrate; D012822:Silicon Dioxide; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D005947:Glucose; D005976:Glutaral