Chitosan/PLA nanoparticles as a novel carrier for the delivery of anthraquinone: synthesis, characterization and in vitro cytotoxicity evaluation.
Designing novel materials for biomedical applications generally require the use of biodegradable materials. This study aims to engineer a biodegradable [chitosan (CS) and poly (lactic acid) (PLA)] as AQ carrier with nanometer dimensions and to evaluate the anticancer potency of the prepared CS/PLA-AQ NPs in human carcinoma (HepG2) cells. CS-PLA complex, which are well dispersed and stable in aqueous solution, was prepared by the precipitation of lactic acid in chitosan solution by dropping method and characterized by SEM, TEM, DLS and FTIR. The results thus displayed that the prepared nanoparticles carried a positive charge and showed the size in the range from 100 to 200 nm. The in vitro (AQ) release study showed that these nanoparticles provided a continuous release of the entrapped AQ for 10 days, and the release behavior was influenced by the pH value of the medium thereby making feasible to develop CS-PLA for enhanced and sustained release of AQ. MTT assay revealed higher cytotoxic efficacy of CS/PLA-AQ NPs than Free AQ in HepG2 cells. Further, the mitochondrial membrane damage indicated by loss of mitochondrial membrane potential and necrotic cell death could be attributed to the increased reactive oxygen species production. Our results also suggest that upon CS/PLA-AQ NPs exposure the cell viability decreased due to apoptosis, as demonstrated by the formation of apoptotic bodies, sub-G1 hypodiploid cells, and DNA fragmentation. Henceforth, CS/PLA-AQ NPs demonstrated a strong antitumor activity in vitro by reducing cell viability, inducing cell necrosis, decreasing the negative surface charge and mitochondrial membrane potential, and fragmenting DNA.
Department of Biochemistry, Sathyabama Dental College and Hospitals, Sathyabama University, Rajiv Gandhi Salai, Sholinganallur, Chennai 119, Tamilnadu, India. jivitha@hotmail.com
D
D
Jeevitha
Kanchana
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Amarnath
D017304:Annexin A5 / Q000032:analysis; D000880:Anthraquinones / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D053938:DNA Fragmentation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D016503:Drug Delivery Systems; D004587:Electrophoresis, Agar Gel; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D009336:Necrosis; D010316:Particle Size; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D013778:Tetrazolium Salts; D013844:Thiazoles
D017304:Annexin A5; D000880:Anthraquinones; D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D004337:Drug Carriers; D011091:Polyesters; D011108:Polymers; D013778:Tetrazolium Salts; D013844:Thiazoles; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan; C022616:thiazolyl blue