Schistosoma mansoni: in vitro schistosomicidal activity and tegumental alterations induced by piplartine on schistosomula.
Schistosomiasis is one of the most important parasitic infections in humans that occur in many tropical and subtropical countries. Currently, the control of schistosomiasis rests with a single drug, praziquantel, which is effective against adult worms but not the larval stages. Recent studies have shown that piplartine, an amide isolated from plants of the genus Piper (Piperaceae), reveals interesting antischistosomal properties against Schistosoma mansoni adult worms. Here, we report the in vitro antischistosomal activity of piplartine on S. mansoni schistosomula of different ages (3 h old and 1, 3, 5, and 7 days old), and examine alterations on the tegumental surface of worms by means of confocal laser scanning microscopy. Piplartine at a concentration of 7.5 μM caused the death of all schistosomula within 120 h. The lethal effect occurred in a dose-dependent manner and was also dependent on the age of the parasite. Microscopy observation revealed extensive tegumental destruction, including blebbing, granularity, and a shorter body length. This report provides the first evidence that piplartine is able to kill schistosomula of different ages and reinforce that piplartine is a promising compound that could be used for the development of new schistosomicidal agent.
Laboratório de Parasitologia, Instituto Butantan, São Paulo, SP, Brazil. josuemoraes@ig.com.br
Josué
J
de Moraes
Carlos
C
Nascimento
Lydia F
LF
Yamaguchi
Massuo J
MJ
Kato
Eliana
E
Nakano
D000818:Animals; D001700:Biomphalaria; D006224:Cricetinae; D007814:Larva / Q000187:drug effects; D008647:Mesocricetus; D018613:Microscopy, Confocal; D031701:Piper / Q000737:chemistry; D010881:Piperidones / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D011223:Praziquantel / Q000494:pharmacology; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000648:ultrastructure; D012556:Schistosomicides / Q000494:pharmacology*; D013997:Time Factors
D010881:Piperidones; D010936:Plant Extracts; D012556:Schistosomicides; C498077:piperlongumine; D011223:Praziquantel