A  novel strategy for identification of 47 pomegranate (Punica granatum)  cultivars using RAPD markers.
DNA marker can be used for precise plant  cultivar identification. However, DNA markers have often not been used  effectively for the identification of plant cultivars due to a lack of an  effective analysis strategy. We used a novel strategy for effective  identification of plant individuals based on a new way of recording DNA  fingerprints of the genotyped plants; a cultivar identification diagram can be  manually generated and used as key reference information for quick  identification of plant and/or seed samples. Forty-seven pomegranate varieties  popularly cultivated in various provinces of China were subjected to RAPD  marker analysis. Using the cultivar identification diagram strategy, they were  clearly separated by the fingerprints of 11 RAPD primers. The utility and  accuracy of the cultivar identification diagram analysis results were confirmed  by the identification of three randomly chosen groups of cultivars among the 47  varieties.
College of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, P.R. China.
Y P
YP
Zhang
H H
HH
Tan
S Y
SY
Cao
X C
XC
Wang
G
G
Yang
J G
JG
Fang
D016172:DNA Fingerprinting; D017931:DNA Primers / Q000378:metabolism; D060146:Ecotype; D005819:Genetic Markers; D005838:Genotype; D029561:Lythraceae / Q000145:classification* / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D015203:Reproducibility of Results
D017931:DNA Primers; D005819:Genetic Markers