Pedobacter luteus sp. nov., isolated from soil.
Two strains of Gram-staining-negative, rod-shaped bacteria that were motile by gliding, N7d-4(T) and B4a-b5, were isolated during a study of culturable bacteria in soil cultivated with potatoes. These isolates grew at 15-37 °C and at pH 6.5-7.0. The major cellular fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), anteiso-C15 : 0, iso-C15 : 0, iso-C17 : 0 3-OH and iso-C17 : 1ω9c. The major polar lipids were phosphatidyl-N-methylethanolamine and phosphatidylethanolamine. The strains contained d-18 : 0 and d-19 : 0 sphingosines. The DNA G+C contents of strains N7d-4(T) and B4a-b5 were 48.5 and 46.9 mol% (HPLC), respectively. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains N7d-4(T) and B4a-b5 were affiliated with Pedobacter species in the family Sphingobacteriaceae. Strains N7d-4(T) and B4a-b5 shared 99.9 % sequence similarity, and the most closely related Pedobacter type strains were Pedobacter composti TR6-06(T) (96.5 and 96.7 % sequence similarity, respectively), P. oryzae N7(T) (95.4 and 95.6 %) and P. caeni LMG 22862(T) (94.0 and 94.4 %). Phenotypic data and phylogenetic inference clearly distinguished the two isolates from other Pedobacter species. Based on these data, the isolates are considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter luteus sp. nov. is proposed. The type strain is N7d-4(T) ( = KCTC 22699(T)  = DSM 22385(T)).
Industrial Bio-materials Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
Hyun-Woo
HW
Oh
Energy Materials and Process, BK 21, Hanyang University, Seoul 133-791, Republic of Korea.|Biological Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
Byung-Chun
BC
Kim
Biological Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
Doo-Sang
DS
Park
Biological Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
Won-Jin
WJ
Jeong
Biological Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
Hyangmi
H
Kim
Biological Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
Kang Hyun
KH
Lee
Industrial Bio-materials Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
Sung Uk
SU
Kim
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D060485:Pedobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D011198:Solanum tuberosum / Q000382:microbiology; D013107:Sphingolipids / Q000032:analysis; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D013107:Sphingolipids; C032000:phosphatidyl-N-monomethylaminoethanol; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7