A water soluble Al3+ selective colorimetric and fluorescent turn-on chemosensor and its application in living cell imaging.
An efficient water soluble fluorescent Al(3+) receptor, 1-[[(2-furanylmethyl)imino]methyl]-2-naphthol (1-H) was synthesized and characterized by physico-chemical and spectroscopic tools along with single crystal X-ray crystallography. High selectivity and affinity of 1-H towards Al(3+) in HEPES buffer (DMSO/water: 1/100) of pH 7.4 at 25 °C showed it to be suitable for detection of intracellular Al(3+) by fluorescence microscopy. Metal ions, viz. alkali (Na(+), K(+)), alkaline earth (Mg(2+), Ca(2+)), and transition-metal ions (Ni(2+), Zn(2+), Cd(2+), Co(2+), Cu(2+), Fe(3+), Cr(3+/6+), Hg(2+)) and Pb(2+), Ag(+) did not interfere. The lowest detection limit for Al(3+) was calculated to be 6.03 × 10(-7) M in 100 mM HEPES buffer (DMSO/water: 1/100). Theoretical calculations have also been included in support of the configuration of the probe-aluminium complex.
Department of Chemistry, The University of Burdwan, Golapbag, Burdwan-713104, India.
Supriti
S
Sen
Titas
T
Mukherjee
Basab
B
Chattopadhyay
Anuradha
A
Moirangthem
Anupam
A
Basu
Jaromir
J
Marek
Pabitra
P
Chattopadhyay
D000535:Aluminum / Q000032:analysis*; D003124:Colorimetry; D056831:Coordination Complexes / Q000737:chemistry; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D008670:Metals / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D009284:Naphthols / Q000138:chemical synthesis / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D056831:Coordination Complexes; D005456:Fluorescent Dyes; D007477:Ions; D008670:Metals; D009284:Naphthols; D014867:Water; D000535:Aluminum; C028405:2-naphthol