[Triterpenoids from Inonotus obliquus protect mice against oxidative damage induced by CCl4].
To investigate the effects of lanosterol (1), inotodiol (2) and trametenolic acid (3) from Inonotus obliquus against oxidative damage induced by CCl4 in mice, 1, 2 and 3 (20, 10 and 5 mg x kg(-1)) were respectively administered to mice, once a day for 3 days. Then the mice were induced to oxidative damage by CCl4 on the third day 30 min after the administration. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) and the content of malondialdehyde (MDA) and reductive glutathione (GSH) in serum and liver homogenate were determined. And the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and interleukin-6 (IL-6) concentration in serum were detected. The results showed that treatment with compound 1, 2 and 3 could significantly increase the activities of SOD, CAT and GSH-PX in serum and liver homogenate. Furthermore, the content of GSH in serum and liver homogenate increased and MDA content decreased markedly. In addition, compound 1, 2 and 3 could significantly inhibit the activities of ALT and AST in serum, and decrease the IL-6 concentration in serum remarkably. So, compound 1, 2 and 3 can protect mice against oxidative stress injury induced by CCl4. Furthermore, compound 1, 2 and 3 can protect cells from damage through inhibition on ALT, AST and the expression of IL-6.
Pharmaceutical Institute of Henan University, Kaifeng 475004, China.
Fen-Qin
FQ
Zhao
Lin
L
Yan
Xian-Hong
XH
Cui
Sheng
S
Lin
Cong
C
Wang
Hui
H
Zhang
Xiao-Yan
XY
Kang
Bian-Sheng
BS
Ji
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride; D002374:Catalase / Q000097:blood / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood; D007810:Lanosterol / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D011124:Polyporaceae / Q000737:chemistry*; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D015850:Interleukin-6; D020011:Protective Agents; D014315:Triterpenes; C517091:inotodiol; D007810:Lanosterol; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione