Tissue augmentation by white blood cell-containing platelet-rich plasma.
Platelet-rich plasma (PRP) is a matrix of fibrin and platelets that releases cytokines that are important in wound healing. PRP is produced from the patient's blood and therefore has less risk of allergic reaction and infection. We have obtained PRP with an enhanced white blood cell  component (W-PRP) by optimizing the centrifugal separation of PRP from plasma. Here we show that injection of W-PRP into the auricle of nude mice gave greater tissue augmentation compared to PRP. Further augmentation occurred when bFGF was added to W-PRP, and there was a significant increase  in the number of α-smooth muscle actin-positive cells in mice treated with W-PRP+bFGF. Our results suggest that W-PRP may have value in cosmetic surgery aimed at rejuvenation of wrinkled and sagging skin. W-PRP injection constitutes a new concept in cell transplantation, in which cells  required for tissue regeneration are induced by cytokines released from the transplanted cells.
Department of Plastic Reconstructive and Aesthetic Surgery, Kijunkai, Yoshikawa Hospital, Sakyo-ku, Kyoto, Japan. smnp99024@nike.eonet.ne.jp
Takeshi
T
Kawazoe
Hak Hee
HH
Kim
D000199:Actins / Q000378:metabolism; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D054644:Ear Auricle / Q000187:drug effects / Q000473:pathology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D007962:Leukocytes / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D053657:Platelet-Rich Plasma; D012038:Regeneration
D000199:Actins; D016207:Cytokines; C544013:alpha-smooth muscle actin, mouse; D016222:Fibroblast Growth Factor 2