The sensitive to freezing3 mutation of Arabidopsis thaliana is a cold-sensitive allele of homomeric acetyl-CoA carboxylase that results in cold-induced cuticle deficiencies.
The sfr3 mutation causes freezing sensitivity in Arabidopsis thaliana. Mapping, sequencing, and transgenic complementation showed sfr3 to be a missense mutation in ACC1, an essential gene encoding homomeric (multifunctional) acetyl-CoA carboxylase. Cuticle permeability was compromised in the sfr3 mutant when plants were grown in the cold but not in the warm. Wax deposition on the inflorescence stem of cold-grown sfr3 plants was inhibited and the long-chain components of their leaf cuticular wax were reduced compared with wild-type plants. Thus, freezing sensitivity of sfr3 appears, from these results, to be due to cuticular deficiencies that develop during cold acclimation. These observations demonstrated the essential role of the cuticle in tolerance to freezing and drought.
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 0EX UK.
Azura
A
Amid
Anna
A
Lytovchenko
Alisdair R
AR
Fernie
Gareth
G
Warren
Glenn J
GJ
Thorlby
D000064:Acclimatization; D000103:Acetyl-CoA Carboxylase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D010641:Phenotype; D018515:Plant Leaves / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D029681:Arabidopsis Proteins; D000103:Acetyl-CoA Carboxylase