Nutritional requirements of the BY series of Saccharomyces cerevisiae strains for optimum growth.
Among the vast variety of Saccharomyces cerevisiae strains, the BY family is particularly important because the widely used deletion collections are based on this background. Here we demonstrate that some standard growth media recipes require substantial modifications to provide optimum growth conditions for auxotrophic BY strains and to avoid growth arrest before glucose is depleted. In addition to the essential supplements that are required to satisfy auxotrophic requirements, we found the four amino acids phenylalanine, glutamic acid, serine, and threonine to be indispensable for optimum growth, despite the fact that BY is 'prototrophic' for these amino acids. Interestingly, other widely used S. cerevisiae strains, such as strains of the CEN.PK family, are less sensitive to lack of the described supplements. Furthermore, we found that the concentration of inositol in yeast nitrogen base is too low to support fast proliferation of yeast cultures until glucose is exhausted. Depletion of inositol during exponential growth induces characteristic changes, namely a decrease in glucose uptake and maximum specific growth rate, increased cell size, reduced viability, and accumulation of lipid storage pools. Thus, several of the existing growth media recipes need to be revised to achieve optimum growth conditions for BY-derived strains.
Institute of Molecular Biosciences, University Graz, Graz, Austria.
Michael
M
Hanscho
David E
DE
Ruckerbauer
Neha
N
Chauhan
Harald F
HF
Hofbauer
Stefan
S
Krahulec
Bernd
B
Nidetzky
Sepp D
SD
Kohlwein
Juergen
J
Zanghellini
Klaus
K
Natter
D000596:Amino Acids / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D050356:Lipid Metabolism; D009172:Mycology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism*
D000596:Amino Acids; D003470:Culture Media; D007294:Inositol; D005947:Glucose