Crucial surviving aspects for vitrified in vitro-produced bovine embryos.
The objective of the present study was to correlate some parameters (cleavage, blastocyst production, quality degree score, total cell number, fresh apoptosis and lipid content) with embryo survival after cryopreservation. A total of 1727 in vitro-produced bovine blastocysts were used to establish the parameters (mean ± standard error of the mean (SEM)) for cleavage (85.6 ± 0.8), blastocyst production (39.9 ± 1.4), quality degree score (1.6 ± 0.1), total cell number (140.1 ± 2.9), fresh apoptosis (20.8 ± 1.1) and lipid content (21.3 ± 0.8 droplets). On the same way 1316 blastocysts were vitrified for the determination of post-cryopreservation embryo survival (49.4 ± 1.9). Fresh apoptosis rate and total lipid droplets value were correlated (P < 0.05) with embryo survival after cryopreservation (r = 0.91 and r = 0.59; respectively). However, cleavage, blastocyst production, quality degree score and total cell number were not correlated (P > 0.05) with embryo cryotolerance (r = 0.23, r = 0.38, r = 0.22 and r = 0.28; respectively). Therefore, the increased lipid content was moderately correlated with apoptosis in vitrified blastocysts. On the other hand, increased apoptosis in fresh blastocysts was strongly correlated with apoptosis in vitrified blastocysts, which indicated that the apoptosis rate in fresh embryos was a better parameter than the lipid content to predict post-vitrification embryo survival.
São Paulo State University - UNESP, School of Veterinary Medicine and Animal Science-FMVZ, Department of Animal Reproduction and Veterinary Radiology, Rubião Jr. s/n°, Botucatu-SP, Brazil.
Mateus José
MJ
Sudano
São Paulo State University - UNESP, School of Veterinary Medicine and Animal Science-FMVZ, Department of Animal Reproduction and Veterinary Radiology Rubião Jr. s/n°, Botucatu-SP, Brazil, 18618-970.
Daniela Martins
DM
Paschoal
São Paulo State University - UNESP, School of Veterinary Medicine and Animal Science-FMVZ, Department of Animal Reproduction and Veterinary Radiology Rubião Jr. s/n°, Botucatu-SP, Brazil, 18618-970.
Tatiana
T
da Silva Rascado
São Paulo State University - UNESP, School of Veterinary Medicine and Animal Science-FMVZ, Department of Animal Reproduction and Veterinary Radiology Rubião Jr. s/n°, Botucatu-SP, Brazil, 18618-970.
Letícia Ferrari
LF
Crocomo
São Paulo State University - UNESP, School of Veterinary Medicine and Animal Science-FMVZ, Department of Animal Reproduction and Veterinary Radiology Rubião Jr. s/n°, Botucatu-SP, Brazil, 18618-970.
Luis Carlos Oña
LC
Magalhães
São Paulo State University - UNESP, School of Veterinary Medicine, Department of Surgical Clinic and Animal Reproduction, Araçatuba-SP, Brazil, 16050-680.
Alício Martins
AM
Junior
Embrapa Southeast Cattle, PO Box 339, 13560-970, São Carlos-SP, Brazil.
Rui
R
Machado
São Paulo State University - UNESP, School of Veterinary Medicine and Animal Science-FMVZ, Department of Animal Reproduction and Veterinary Radiology, Rubião Jr. s/n°, Botucatu-SP, Brazil.
Fernanda
F
da Cruz Landim-Alvarenga
D000818:Animals; D017209:Apoptosis; D001755:Blastocyst / Q000166:cytology* / Q000502:physiology; D002417:Cattle / Q000196:embryology* / Q000378:metabolism; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D066298:In Vitro Techniques; D008055:Lipids / Q000032:analysis; D058989:Vitrification
D008055:Lipids