[Chemical constituents of Halenia elliptica].
OBJECTIVE
To study the chemical constituents of Halenia elliptica.


METHODS
The air-dried whole plants of Halenia elliptica were extracted with 90% EtOH. The EtOH extract was condensed to a small amount of volume and extracted with petroleum ether, EtOAc and n-BuOH, successively. The compounds were isolated and purified by column chromatography from the EtOAc fraction, and identified based on spectral analyses (MS, 1H-NMR, 13C-NMR).


RESULTS
12 compounds were isolated from H. elliptica, and characterized as 8-hydroxy-2-methylchromone (1), 5-methoxy-2-methylchromone (2), 7-epi-vogeloside (3), coniferl aldehyde (4), sinapaldehyde (5), norbellidifolin (6), 1-hydroxyl-2,3,4,6-tetramethoxyxanthone (7), 1-hydroxyl-2,3,4,7-tetramethoxyxanthone (8), 1-hydroxyl-2,3,5-trimethoxyxanthone (9), together with azelaic acid, beta-sitosterol, and oleanolic acid.


CONCLUSIONS
Compounds 1, 2 were new natural compounds and compounds 3-6, 10 were obtained from H. elliptica for the first time and compound 6 showed inhibitory activities against HBsAg and HBeAg secretion with IC50 value of 0.77 and < 0.62 mmol x L(-1), respectively.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
Hongling
H
Wang
Hao
H
Chen
Chang'an
C
Geng
Xuemei
X
Zhang
Yunbao
Y
Ma
Zhiyong
Z
Jiang
Jijun
J
Chen
D000171:Acrolein / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D002845:Chromatography; D003998:Dicarboxylic Acids / Q000032:analysis / Q000302:isolation & purification; D029965:Gentianaceae / Q000737:chemistry*; D057889:Iridoid Glycosides / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009828:Oleanolic Acid / Q000032:analysis / Q000302:isolation & purification; D010936:Plant Extracts / Q000302:isolation & purification*; D012855:Sitosterols / Q000032:analysis / Q000302:isolation & purification; D044004:Xanthones / Q000032:analysis* / Q000302:isolation & purification
D003998:Dicarboxylic Acids; D057889:Iridoid Glycosides; D010936:Plant Extracts; D012855:Sitosterols; D044004:Xanthones; C075386:sinapaldehyde; C025473:gamma-sitosterol; D009828:Oleanolic Acid; D000171:Acrolein; C010038:azelaic acid