Molecular characterization of woodchuck interleukin-10 receptor and enhanced function of specific T cells from chronically infected woodchucks following its blockade.
Interleukin 10 (IL-10) is a pleiotropic cytokine acting on a variety of immune cells through the cell surface receptor (IL-10R). It has been suggested to resuscitate antiviral immunity by interfering with IL-10/IL-10R pathway. The woodchuck model infected by woodchuck hepatitis virus (WHV) represents an informative animal model to study hepatitis B virus (HBV) infection. In this study, the woodchuck IL-10R (wIL-10R) was molecularly cloned and characterized, showing high similarity of its nucleotide and amino acid sequences to that of other mammalian species. The expression level of wIL-10R mRNA in woodchuck peripheral blood mononuclear cells was significantly increased in acute WHV infection but down-regulated during chronic WHV infection. Specific rabbit antibodies against wIL-10R were prepared and showed the ability to enhance the proliferation and degranulation of specific T-cells from chronically WHV-infected woodchucks in vitro. The present work on wIL-10R provided a good basis for future preclinical studies on therapeutic approaches for chronic HBV infection.
Department of Microbiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Min
M
Jiang
Jia
J
Liu
Ejuan
E
Zhang
Zhongji
Z
Meng
Baoju
B
Wang
Michael
M
Roggendorf
Dongliang
D
Yang
Mengji
M
Lu
Yang
Y
Xu
D000208:Acute Disease; D000595:Amino Acid Sequence; D000818:Animals; D015550:Cell Degranulation; D049109:Cell Proliferation; D002478:Cells, Cultured; D015870:Gene Expression; D017919:Hepatitis B Virus, Woodchuck; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D016753:Interleukin-10 / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D008392:Marmota / Q000235:genetics* / Q000276:immunology / Q000821:virology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000097:blood; D011817:Rabbits; D053701:Receptors, Interleukin-10 / Q000235:genetics* / Q000276:immunology; D012376:Rodent Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology; D017386:Sequence Homology, Amino Acid
D012333:RNA, Messenger; D053701:Receptors, Interleukin-10; D016753:Interleukin-10