[Preparation and biocompatibility of porcine skeletal muscle acellular matrix for adipose tissue engineering].
OBJECTIVE
Extracellular matrix is one of the focus researches of the adipose tissue engineering. To investigate the appropriate method to prepare the porcine skeletal muscle acellular matrix and to evaluate the biocompatibility of the matrix.


METHODS
The fresh skeletal muscle tissues were harvested from healthy adult porcine and were sliced into 2-3 mm thick sheets, which were treated by hypotonic-detergent method to remove the cells from the tissue. The matrix was then examined by histology, immunohistochemistry, and scanning electron microscopy. The toxic effects of the matrix were tested by MTT. Human adipose-derived stem cells (hADSCs) were isolated from adipose tissue donated by patients with breast cancer, and identified by morphology, flow cytometry, and differentiation ability. Then, hADSCs of passage 3 were seeded into the skeletal muscle acellular matrix, and cultured in the medium. The cellular behavior was assessed by calcein-AM (CA) and propidium iodide (PI) staining at 1st, 3rd, 5th, and 7th days after culturing.


RESULTS
Histology, immunohistochemistry, and scanning electron microscopy showed that the muscle fibers were removed completely with the basement membrane structure; a large number of collagenous matrix presented as regular network, porous-like structure. The cytotoxicity score of the matrix was grade 1, which meant that the matrix had good cytocompatibility. The CA and PI staining showed the seeded hADSCs had the potential of spread and proliferation on the matrix.


CONCLUSIONS
Porcine skeletal muscle acellular matrix has good biocompatibility and a potential to be used as an ideal biomaterial scaffold for adipose tissue engineering.
Department of Thyroid & Breast Surgery, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P.R.China.
Chunxiang
C
Tian
Xuejiao
X
Fan
Xiaohe
X
Chen
Li
L
Deng
Tingwu
T
Qin
Jingcong
J
Luo
Xiuqun
X
Li
Qing
Q
Lü
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001672:Biocompatible Materials; D049109:Cell Proliferation; D002478:Cells, Cultured; D005109:Extracellular Matrix; D006801:Humans; D007150:Immunohistochemistry; D018482:Muscle, Skeletal / Q000166:cytology*; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials