Effect of eriodictyol on glucose uptake and insulin resistance in vitro.
Eriodictyol [2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-2,3-dihydrochromen-4-one] is a flavonoid with anti-inflammatory and antioxidant activities. Because inflammation and oxidative stress play critical roles in the pathogenesis of diabetes mellitus, the present study was designed to explore whether eriodictyol has therapeutic potential for the treatment of type 2 diabetes. The results show that eriodictyol increased insulin-stimulated glucose uptake in both human hepatocellular liver carcinoma cells (HepG2) and differentiated 3T3-L1 adipocytes under high-glucose conditions. Eriodictyol also up-regulated the mRNA expression of peroxisome proliferator-activated receptor γ2 (PPARγ2) and adipocyte-specific fatty acid-binding protein (aP2) as well as the protein levels of PPARγ2 in differentiated 3T3-L1 adipocytes. Furthermore, it reactivated Akt in HepG2 cells with high-glucose-induced insulin resistance. This response was strongly inhibited by pretreatment with the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002, indicating that eriodictyol increased Akt phosphorylation by activating the PI3K/Akt pathway. These results imply that eriodictyol can increase glucose uptake and improve insulin resistance, suggesting that it may possess antidiabetic properties.
Department of Pharmacology, Chungnam National University College of Pharmacy, Daejeon 305-764, Republic of Korea.
Wei-Yun
WY
Zhang
Jung-Jin
JJ
Lee
Yohan
Y
Kim
In-Su
IS
Kim
Joo-Hui
JH
Han
Sang-Gil
SG
Lee
Min-Ju
MJ
Ahn
Sang-Hyuk
SH
Jung
Chang-Seon
CS
Myung
D041721:3T3-L1 Cells; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D044950:Flavanones / Q000008:administration & dosage*; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007333:Insulin Resistance; D051379:Mice; D047492:Peroxisome Proliferator-Activated Receptors / Q000235:genetics / Q000378:metabolism
D050556:Fatty Acid-Binding Proteins; D044950:Flavanones; D007004:Hypoglycemic Agents; D047492:Peroxisome Proliferator-Activated Receptors; D005947:Glucose; C007619:eriodictyol