Screening and  identification of peritoneal metastasis-related genes of gastric adenocarcinoma  using a cDNA microarray.
With  the aim of identifying peritoneal metastasis-related genes in gastric cancer,  we performed a broad analysis of differential gene expression between the parental  cell line GC9811 and its highly metastatic peritoneal counterpart, cell line  GC9811-P. Two fluorescent cDNA probes, labeled with Cy3 and Cy5 dyes, were  prepared from GC9811 and GC9811-P mRNA samples by the reverse transcription  method. The two color probes were then mixed and hybridized to a cDNA chip  constructed with double-dots from 11,901 human genes; this was scanned at two  wavelengths. The experiment was repeated twice. In GC9811-P cells, 218 genes  were upregulated and 30 genes were downregulated compared with the parental  cell lines. Some selected genes were confirmed by RT-PCR and Western blot; we  found that S100A4 and CTNNB1 were upregulated and PTEN was downregulated in  GC9811-P cells. Identification of these differentially expressed genes could  contribute to disclose the molecular mechanisms involved and provide new  targets for therapeutic intervention to avoid peritoneal dissemination of  gastric adenocarcinoma.
State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, XiJing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
F H
FH
Bai
N J
NJ
Wang
J
J
Wang
L
L
Yang
F M
FM
Zhang
F
F
Yin
J
J
Liang
K C
KC
Wu
D M
DM
Fan
D000230:Adenocarcinoma / Q000235:genetics*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*