Enhanced transformation of TNT by Arabidopsis plants expressing an old yellow enzyme.
2,4,6-Trinitrotoluene (TNT) is released in nature from manufacturing or demilitarization facilities, as well as after the firing or detonation of munitions or leakage from explosive remnants of war. Environmental contamination by TNT is associated with human health risks, necessitating the development of cost-effective remediation techniques. The lack of affordable and effective cleanup technologies for explosives contamination requires the development of better processes. In this study, we present a system for TNT phytoremediation by overexpressing the old yellow enzyme (OYE3) gene from Saccharomyces cerevisiae. The resulting transgenic Arabidopsis plants demonstrated significantly enhanced TNT tolerances and a strikingly higher capacity to remove TNT from their media. The current work indicates that S. cerevisiae OYE3 overexpression in Arabidopsis is an efficient method for the phytoremoval and degradation of TNT. Our findings have the potential to provide a suitable remediation strategy for sites contaminated by TNT.
Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, China.
Bo
B
Zhu
Ri-He
RH
Peng
Xiao-Yan
XY
Fu
Xiao-Fen
XF
Jin
Wei
W
Zhao
Jing
J
Xu
Hong-Juan
HJ
Han
Jian-Jie
JJ
Gao
Zhi-Sheng
ZS
Xu
Lin
L
Bian
Quan-Hong
QH
Yao
D060054:Agrobacterium / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D001673:Biodegradation, Environmental; D053834:Explosive Agents / Q000378:metabolism*; D006801:Humans; D009252:NADPH Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*; D014161:Transduction, Genetic; D014303:Trinitrotoluene / Q000378:metabolism*
D053834:Explosive Agents; D029701:Saccharomyces cerevisiae Proteins; D012989:Soil Pollutants; D014303:Trinitrotoluene; D009252:NADPH Dehydrogenase