Effects of phosphorus concentration and light intensity on the biomass composition of Arthrospira (Spirulina) platensis.
This paper presents the effects of various phosphorus concentrations (10, 50, 250 and 500 mg l(-1) K(2)HPO(4)) on the biomass production and composition of Arthrospira (Spirulina) platensis in relation to light intensity (24, 42 and 60 μE m(-2) s(-1)). The maximum biomass production was 3,592 ± 392 mg l(-1) and this was observed in 250 mg l(-1) K(2)HPO(4) at 60 μE m(-2) s(-1) light intensity after 32 days of cultivation. A maximum specific growth rate (μ(max)) of 0.55 d(-1) was obtained in 500 mg l(-1) K(2)HPO(4) at 60 μE m(-2) s(-1). The protein, lipid and chlorophyll contents of the biomass varied from 33.59 to 60.57 %, 5.34 to 13.33 % and 0.78 to 2.00 %, respectively. The most significant finding was that phosphorus limitation (10 mg l(-1) K(2)HPO(4)) caused a drastic increase of the carbohydrate content (59.64 %). The effect of phosphorus limitation on the carbohydrate content was independent of the light intensity. The accumulated carbohydrates are proposed to be used as substrate for biofuel generation via one of the appropriate biomass energy conversion technologies. Also, it was observed that phosphorus removal is a function of biomass density, phosphorus concentration and light intensity.
Department of Natural Resources Management and Agricultural Engineering, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece. markoug@aua.gr
Giorgos
G
Markou
Iordanis
I
Chatzipavlidis
Dimitris
D
Georgakakis
D001426:Bacterial Proteins / Q000378:metabolism; D056804:Biofuels; D018533:Biomass; D050260:Carbohydrate Metabolism; D002734:Chlorophyll / Q000378:metabolism; D008027:Light; D050356:Lipid Metabolism; D010758:Phosphorus / Q000378:metabolism / Q000494:pharmacology*; D053545:Spirulina / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects
D001426:Bacterial Proteins; D056804:Biofuels; D002734:Chlorophyll; D010758:Phosphorus