Assessment of the relevance of supported planar bilayers for modeling specific interactions between glycodendrimeric porphyrins and retinoblastoma cells.
Glycodendrimeric porphyrins seem promising photosensitizers usable in photodynamic therapy. Evidence of their ability to interact with an artificial supported bilayer membrane exhibiting a model sugar receptor has been previously shown. In the present work, the interaction of the glycodendrimeric porphyrins with retinoblastoma cells bearing the actual sugar receptor has been assessed by both classical cell cultures and an original approach using the quartz crystal microbalance (QCM-D). Our results showed that unlike cell cultures, QCM-D allowed analyzing the mechanism of interaction of the glycodendrimeric porphyrins with the sugar receptor. Not only was molecular recognition demonstrated, but our methodology also proved efficient to discriminate between the studied compounds, depending on the presence of carbohydrate, and the spacer length.
Univ Paris-Sud 11, Laboratoire de Physico-chimie des Surfaces, Châtenay-Malabry cedex, France.
Ali
A
Makky
Katia
K
Daghildjian
Jean-Philippe
JP
Michel
Philippe
P
Maillard
Véronique
V
Rosilio
D002241:Carbohydrates / Q000737:chemistry*; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000378:metabolism*; D006801:Humans; D008051:Lipid Bilayers; D008081:Liposomes; D008954:Models, Biological; D011166:Porphyrins / Q000378:metabolism*; D012175:Retinoblastoma / Q000378:metabolism* / Q000473:pathology; D012822:Silicon Dioxide / Q000378:metabolism
D002241:Carbohydrates; D050091:Dendrimers; D008051:Lipid Bilayers; D008081:Liposomes; D011166:Porphyrins; D012822:Silicon Dioxide