Alkaloids from some amaryllidaceae species and their cholinesterase activity.
Alkaloid extracts of four Amaryllidaceae species were studied with respect to their acetylcholinesterase and butyrylcholinesterase inhibitory activity and alkaloid patterns. Twenty-one alkaloids were determined by GC/MS, and seventeen of them identified from their mass spectra and retention times. The GC/MS analysis of the alkaloid extract of Nerine filamentosa is the first phytochemical investigation of this species. Promising erythrocytic acetylcholinesterase inhibitory activity was demonstrated by the alkaloid extracts of Narcissus poeticus var recurvus, Nerine filifolia and N. filamentosa (IC(50,HuAChE) = 6.0 +/- 0.1 microg/mL; IC(50,HuAChE) = 18.5 +/- 0.8 microg/mL, IC(50,HuAChE) = 21.6 +/- 1.1 microg/mL). The most potent inhibitory activity against serum butyrylcholinesterase was shown by extracts of Sternbergia lutea and Nerinefilamentosa (IC(50,HuBuChE) = 3.7 +/- 0.1 microg/mL; IC(50.HuBuChE) = 13.0 +/- 0.7 microg/mL).
ADINACO Research Group, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic. cahlikova@fafcuni.cz
Lucie
L
Cahlíková
Nina
N
Benesová
Katerina
K
Macáková
Radim
R
Kucera
Václav
V
Hrstka
Jirí
J
Klimes
Ludek
L
Jahodár
Lubomír
L
Opletal
D000470:Alkaloids / Q000032:analysis* / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D019668:Liliaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*
D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts