A green and bio-inspired process to afford durable anti-biofilm properties to stainless steel.
A bio-inspired durable anti-biofilm coating was developed for industrial stainless steel (SS) surfaces. Two polymers inspired from the adhesive and cross-linking properties of mussels were designed and assembled from aqueous solutions onto SS surfaces to afford durable coatings. Trypsin, a commercially available broad spectrum serine protease, was grafted as the final active layer of the coating. Its proteolytic activity after long immersion periods was demonstrated against several substrata, viz. a synthetic molecule, N-α-benzoyl-DL-arginine-p-nitroanilide hydrochloride (BAPNA), a protein, FTC-casein, and Gram-positive biofilm forming bacterium Staphylococcus epidermidis.
Center for Education and Research on Macromolecules, Department of Chemistry-CERM, University of Liege, Liege, Belgium.
E
E
Faure
C
C
Vreuls
C
C
Falentin-Daudré
G
G
Zocchi
C
C
Van de Weerdt
J
J
Martial
C
C
Jérôme
A-S
AS
Duwez
C
C
Detrembleur
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D058491:Bacterial Load; D001586:Benzoylarginine Nitroanilide / Q000737:chemistry; D018441:Biofilms / Q000187:drug effects; D057219:Biofouling / Q000517:prevention & control*; D002364:Caseins / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004295:Dihydroxyphenylalanine / Q000737:chemistry; D004789:Enzyme Activation; D005452:Fluoresceins / Q000737:chemistry; D055772:Green Chemistry Technology; D007211:Indoles / Q000737:chemistry; D050296:Microbial Viability; D008856:Microscopy, Fluorescence; D011108:Polymers / Q000737:chemistry; D059748:Proteolysis; D013193:Stainless Steel / Q000737:chemistry*; D013212:Staphylococcus epidermidis / Q000187:drug effects*; D055672:Static Electricity; D013499:Surface Properties; D014357:Trypsin / Q000737:chemistry
D000900:Anti-Bacterial Agents; D002364:Caseins; D003432:Cross-Linking Reagents; D005452:Fluoresceins; D007211:Indoles; D011108:Polymers; C044337:fluorescein thiocarbamoyl casein; C568283:polydopamine; D013193:Stainless Steel; D004295:Dihydroxyphenylalanine; D001586:Benzoylarginine Nitroanilide; D014357:Trypsin