[The protection effects of resveratrol on irradiated human pneumonic cell lines and its mechanism].
OBJECTIVE
This study was to explore whether resveratrol could protect human bronchial epithelial cells (HBE) and human fetal lung fibroblasts (MRC5) from radiation injury and to investigate its potential HBE and MRC5 were divided into four groups: Group 1 (Vehicle), control group, only mechanism.


METHODS
treated with vehicle; Group 2 (resveratrol, Res), the resveratrol group, treated with 5 micromol/L resveratrol; Group 3 (RT+Vehicle), the X-ray irradiation group, only subjected to irradiation of 20 Gy X-ray; Group 4 (RT+Res), the combination therapy group, 2 hours before X-ray treatment (20 Gy, 8. 33 Gy/min for 144 s), 5 micromol/L resveratrol was added to the cells. Several experimental methods were used to observe cellular morphology, ultrastructure, viability, DNA damage, apoptosis, and to determine the change of oxidative stress indexes such as reactive oxygen species (ROS), malondialdehyde (MDA), total glutathione (GSH) and superoxide dismutase (SOD).


RESULTS
X-ray could induce HBE and MRC5 cell injury. Resveratrol could significantly ease the morphological and ultrastructure injury, relieve the decrease of cellular viability and the damage of DNA, and reduce cellular apoptosis. Besides, oxidative stress indexes including ROS, MDA, GSH, SOD were improved by resveratrol after irradiation.


CONCLUSIONS
Resveratrol protect HBE and MRC5 from radiation injury, which is related to the alleviation of oxidative stress injury.
Department of Thoracic Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Xiao-yue
XY
Fu
Jian-xin
JX
Xue
Lin
L
Zhou
Yong-sheng
YS
Wang
Mei-juan
MJ
Huang
Lu
L
Li
You
Y
Lu
D017209:Apoptosis; D001980:Bronchi / Q000166:cytology*; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000528:radiation effects*; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects; D006801:Humans; D008168:Lung / Q000166:cytology; D018384:Oxidative Stress; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D011837:Radiation-Protective Agents; D013267:Stilbenes; D000077185:Resveratrol