Paenibacillus hordei sp. nov., isolated from naked barley in Korea.
A Gram-staining positive, facultative aerobic bacterium, designated strain RH-N24(T), was isolated from naked barley in South Korea. Cells of the isolate were observed to be motile rods by means of peritrichous flagella and showed catalase-positive and oxidase-negative reactions. Growth of strain RH-N24(T) was observed at 4-40 °C (optimum: 35-37 °C) and at pH 5.0-9.0 (optimum: pH 6.0-7.0). Chemotaxonomic data (major isoprenoid quinone: MK-7; DNA G + C content: 53.5 mol %; cell wall type: A1γ-meso-diaminopimelic acid; major fatty acids: anteiso-CB(15:0) and CB(16:0B)) supported the affiliation of the isolate to the genus Paenibacillus. The major cellular polar lipids were identified as phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified polar lipids. Phylogenetic analysis based on 16S rRNA gene sequences also supported the conclusion that strain RH-N24(T) belonged to the genus Paenibacillus. Comparative 16S rRNA gene sequence analysis showed that strain RH-N24(T) was most closely related to Paenibacillus                 hunanensis FeL05(T) and Paenibacillus                 illinoisensis NRRL NRS-1356(T) with similarities of 94.64 and 94.54 %, respectively. On the basis of phenotypic and molecular properties, strain RH-N24(T) represents a novel species within the genus Paenibacillus for which the name Paenibacillus                 hordei sp. nov. is proposed. The type strain is RH-N24(T) (=KACC 15511(T) = JCM 17570(T)).
School of Biological Sciences, Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Jeong Myeong
JM
Kim
Se Hee
SH
Lee
Seung Hyeon
SH
Lee
Eun Jin
EJ
Choi
Che Ok
CO
Jeon
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella / Q000502:physiology; D001467:Hordeum / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; D002374:Catalase