Comparative analysis of the invasion-associated genes expression pattern in first trimester trophoblastic (HTR-8/SVneo) and JEG-3 choriocarcinoma cells.
Several cellular models of trophoblast have been proposed to understand their invasion. We had reported that JEG-3 and HTR-8/SVneo cells show differential invasive behavior in response to IL-11 treatment. So, the present study aims to compare the expression of invasion-associated molecules in these two cell lines by performing cDNA microarray followed by quantitative RT-PCR. We have observed that HTR-8/SVneo cells have significantly higher invasiveness than JEG-3 cells, which might be due to higher expression of proteases and signaling intermediates of JAK/STAT and MAPK signaling pathways. Like extravillous trophoblasts (EVTs), a higher expression of functionally significant proteases like MMP1, MMP2, MMP9, PLAU etc in HTR-8/SVneo cells, project them as a close mimic of EVTs under in vitro conditions.
Reproductive Cell Biology Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India.
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P
Suman
S K
SK
Gupta
D045744:Cell Line, Tumor; D002822:Choriocarcinoma / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D017370:Interleukin-11 / Q000494:pharmacology; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis; D014327:Trophoblasts / Q000378:metabolism*
D017370:Interleukin-11; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1