Effects of laser and LED radiation on mitochondrial respiration in experimental endotoxic shock.
Effects of low-level laser therapy (442 and 532 nm) and LED radiation (650 nm) on mitochondrial respiration in experimental endotoxic shock have been studied. A model of experimental endotoxic shock in rats was obtained by intraperitoneal injection of lipopolysaccharide B. It was found that low-level laser therapy and LED radiation dramatically affected the rate of mitochondrial respiration in third and fourth states both in LPS-treated animals and in control experiments. The maximal increase of the mitochondrial respiration rate (of about 40 %) in LPS-treated animals was observed when blue laser was applied at the dose of 6 J/cm(2). Measurements of inner mitochondrial membrane surface potential with fluorescence probe JC-1 in LPS-treated rats showed in approximately 10 % decrease of potential in LPS-treated animals compared to control.
Department of Medical Biophysics, NI Pirogov Russian State Medical University, 1 Ostrovityanov str., 117997, Moscow, Russia. evgenii.sci@gmail.com
E A
EA
Buravlev
T V
TV
Zhidkova
Y A
YA
Vladimirov
A N
AN
Osipov
D000818:Animals; D004195:Disease Models, Animal; D054020:Lasers, Gas / Q000627:therapeutic use; D053844:Lasers, Solid-State / Q000627:therapeutic use; D008027:Light; D008070:Lipopolysaccharides / Q000633:toxicity; D028022:Low-Level Light Therapy; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000528:radiation effects; D008928:Mitochondria / Q000378:metabolism / Q000528:radiation effects; D010101:Oxygen Consumption / Q000528:radiation effects; D010789:Phototherapy; D051381:Rats; D012772:Shock, Septic / Q000378:metabolism / Q000532:radiotherapy* / Q000628:therapy*
D008070:Lipopolysaccharides