[A new method of microbial prospecting for oil and gas based on BIOLOG metabolic fingerprinting analysis].
OBJECTIVE
Establishing a new method of microbial prospecting for oil and gas based on microbial community metabolic function.


METHODS
In this study, 45 shallow reservoir soil samples and 25 non-reservoir soil samples were collected. Carbon catabolic activity of the microbial community was measured by biolog microplate, and microbial anomaly of reservoir was judged by discriminant function.


RESULTS
We screened 10 typical carbon sources of 95 carbon sources which could reflect the differences of reservoir and non-reservoir soil microbial community structure and made the validation test set to experimental zone and controlled zone samples using the discriminant function. The discriminate rate of reservoir and non-reservoir were 97.8% and 100% respectively and the overall accuracy was 98.6%.


CONCLUSIONS
Biolog technology provided a highly efficient and precise method for preliminarily predicting oil and gas reservoir.
College of Life Science and Technology of Xinjiang University, Urumqi 830046, China. liaoxianyan@gmail.com
Xianyan
X
Liao
Tao
T
Zhang
Jun
J
Zeng
Jian
J
Sun
Wen
W
Hu
Kai
K
Lou
D002244:Carbon / Q000032:analysis* / Q000378:metabolism*; D005740:Gases / Q000737:chemistry; D009821:Oils / Q000737:chemistry; D012988:Soil Microbiology
D005740:Gases; D009821:Oils; D002244:Carbon