Cell origins and diagnostic accuracy of interleukin 27 in pleural effusions.
The objective of the present study was to investigate the presence of interleukin (IL)-27 in pleural effusions and to evaluate the diagnostic significance of pleural IL-27. The concentrations of IL-27 were determined in pleural fluids and sera from 68 patients with tuberculous pleural effusion, 63 malignant pleural effusion, 22 infectious pleural effusion, and 21 transudative pleural effusion. Flow cytometry was used to identify which pleural cell types expressed IL-27. It was found that the concentrations of pleural IL-27 in tuberculous group were significantly higher than those in malignant, infectious, and transudative groups, respectively. Pleural CD4(+) T cells, CD8(+) T cells, NK cells, NKT cells, B cells, monocytes, macrophages, and mesothelial cells might be the cell sources for IL-27. IL-27 levels could be used for diagnostic purpose for tuberculous pleural effusion, with the cut off value of 1,007 ng/L, IL-27 had a sensitivity of 92.7% and specificity of 99.1% for differential diagnosing tuberculous pleural effusion from non-tuberculous pleural effusions. Therefore, compared to non-tuberculous pleural effusions, IL-27 appeared to be increased in tuberculous pleural effusion. IL-27 in pleural fluid is a sensitive and specific biomarker for the differential diagnosing tuberculous pleural effusion from pleural effusions with the other causes.
Key Laboratory of Pulmonary Diseases of Health Ministry, Department of Respiratory and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Wei-Bing
WB
Yang
Qiu-Li
QL
Liang
Zhi-Jian
ZJ
Ye
Chun-Mi
CM
Niu
Wan-Li
WL
Ma
Xian-Zhi
XZ
Xiong
Rong-Hui
RH
Du
Qiong
Q
Zhou
Jian-Chu
JC
Zhang
Huan-Zhong
HZ
Shi
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002478:Cells, Cultured; D003937:Diagnosis, Differential; D005122:Exudates and Transudates / Q000378:metabolism; D005260:Female; D006801:Humans; D007378:Interleukins / Q000097:blood / Q000378:metabolism*; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010996:Pleural Effusion / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D016066:Pleural Effusion, Malignant / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D012372:ROC Curve; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D055815:Young Adult
D015415:Biomarkers; D007378:Interleukins; C464565:MYDGF protein, human