The use of N-ethylmaleimide for mass spectrometric detection of homocysteine fractions in blood plasma.
Fraction analysis of homocysteine in biological fluids is important for the diagnosis and studies of cardiovascular, nervous, urological, and other diseases. Measurements of total, free, and reduced homocysteine by mass spectrometry with HPLC by means of its modification with N-ethylmaleimide are proposed. The linearity and detection threshold were 0.025-10 and 0.001 μM, respectively, for reduced homocysteine fraction and 0.2-100 and 0.1 μM, respectively, for the rest fractions. The accuracy and reproducibility of the method were within 12%.
Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia.
A V
AV
Ivanov
B P
BP
Luzyanin
A A
AA
Kubatiev
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005033:Ethylmaleimide / Q000494:pharmacology*; D006710:Homocysteine / Q000032:analysis* / Q000097:blood; D013058:Mass Spectrometry / Q000379:methods*; D010949:Plasma / Q000737:chemistry*; D011817:Rabbits; D015203:Reproducibility of Results
D006710:Homocysteine; D005033:Ethylmaleimide