[Impact of 2, 3-dihydrocyclopentacoumarins to growth of transgenic hairy roots of Polygonum multiflorum and accumulation of stilbene glucosides].
OBJECTIVE
To investigate the influence of 2,3-dihydrocyclopenta coumarins to the growth of transgenic hairy root of Polygonum multiflorum and the accumulation of active ingredient stilbene glucoside in the culture.


METHODS
In different culture time, 2,3-dihydrocyclopenta coumarin compounds were administrated to the hairy roots. The yield of stilbene glucoside and the growth of the hairy roots were determined after some period of co-culture. Through the investigation of the different concentrations of the 2,3-dihydrocyclopentacoumarin, and sub-culture times, the optimal experimental conditions were established.


RESULTS
The optimal administration time of the 2,3-dihydrocyclopentacoumarin was on the fourth day, the optimal concentration of 2,3-dihydrocyclopentacoumarin was 0.025 g x L(-1). Under this condition, the growth of the hairy root greatly increased. Comparing with control group, the accumulation of stilbene glucoside was increased about four times.


CONCLUSIONS
2, 3-dihydrocyclopentacoumarins could promote the hairy hair root growth and stimulate the stilbene glucoside accumulation.
Biotechnology Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China.
Ling
L
Liu
Liangbin
L
Zhou
Minghua
M
Zhang
Rongmin
R
Yu
D003374:Coumarins / Q000494:pharmacology*; D005960:Glucosides / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D028502:Polygonum / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D013267:Stilbenes / Q000378:metabolism*
C489707:2',3',4',5'-tetrahydroxystilbene-2-O-beta-D-glucoside; D003374:Coumarins; D005960:Glucosides; D013267:Stilbenes