How similar are inbred rats? The influence of anatomical variations, shipment and sampling time on experimental surgery.
Variations among inbred rats in terms of anatomy and routine laboratory values can potentially blur surgical experimental results. Therefore, a retrospective analysis aiming at investigating hepatic and perihepatic anatomical variations, liver weight, body weight, liver weight/body weight ratio (LBWR), variations in routine laboratory values, and the influence of shipment and repeated sampling was performed. In our study, liver weight of rats seemed to be strain-specific. LBWR was weakly and negatively correlated with body weight in rats. A statistically significant difference in routine blood tests was found among normal rats grouped by different body weight or shipment. Weekly repeated sampling from the same rats revealed a statistically significant difference in a blood test. In conclusion, the fact that variation among rats or their environment can blur the results of a surgical experimental study should be kept in mind.
Research Group for Experimental Surgery, Department of General, Visceral and Transplantation Surgery, University Hospital Essen, University of Duisburg and Essen, Essen, Germany.
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Jin
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Huang
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Zhang
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Dirsch
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Dahmen
D063405:Anatomic Variation; D000818:Animals; D001772:Blood Cell Count; D008099:Liver / Q000033:anatomy & histology* / Q000098:blood supply; D016031:Liver Transplantation; D008297:Male; D009929:Organ Size; D051381:Rats; D011917:Rats, Inbred Lew / Q000033:anatomy & histology* / Q000097:blood / Q000601:surgery; D012016:Reference Values; D012189:Retrospective Studies