Comparison of the structural characterization and biological activity of acidic polysaccharides from Cordyceps militaris cultured with different media.
Two acidic polysaccharide fractions, CM-jd-CPS2 and CM-jd(Y)-CPS2, were isolated from the fruiting bodies of cultured Cordyceps militaris grown on solid rice medium and silkworm pupa, respectively, by hot-water extraction, ethanol precipitation and fractionation using ion-exchange column (DEAE-cellulose-52) and gel-filtration column (Sephadex G-100) chromatography. Their structural characterizations were performed by gas chromatography and fourier-transform infrared spectroscopy. Some differences existed between their structures, which indicated that culture media could influence the structure of polysaccharides of C. militaris. The antioxidant activities of CM-jd-CPS2 and CM-jd(Y)-CPS2 were evaluated by various methods in vitro. They had strong 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity and ferrous ion-chelating capacity, but moderate reducing power. The antioxidant activities of CM-jd(Y)-CPS2 were slightly higher than those of CM-jd-CPS2. These two acidic fractions were evaluated for proliferation of mouse splenocyte activity in vitro. They both possessed does-dependent mitogenic effects on mouse splenocytes, and could synergistically promote murine T- and B-lymphocytes induced by Con A and LPS. CM-jd(Y)-CPS2 exhibited stronger stimulatory activities upon immunomodulation than CM-jd-CPS2. These results are beneficial for the interpretation of the connection between polysaccharide structures and their biological activities.
Jiangsu University of Science and Technology, Zhenjiang 212018, China.
Fengyao
F
Wu
Hui
H
Yan
Xiaoning
X
Ma
Junqiang
J
Jia
Guozheng
G
Zhang
Xijie
X
Guo
Zhongzheng
Z
Gui
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D012831:Bombyx / Q000382:microbiology; D049109:Cell Proliferation / Q000187:drug effects; D002614:Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D032661:Cordyceps / Q000737:chemistry* / Q000254:growth & development*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D051379:Mice; D012275:Oryza / Q000382:microbiology; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D011679:Pupa / Q000382:microbiology; D017550:Spectroscopy, Fourier Transform Infrared
D000975:Antioxidants; D002614:Chelating Agents; D016166:Free Radical Scavengers; D011134:Polysaccharides