Satiety hormone and metabolomic response to an intermittent high energy diet differs in rats consuming long-term diets high in protein or prebiotic fiber.
Large differences in the composition of diet between early development and adulthood can have detrimental effects on obesity risk. We examined the effects of an intermittent high fat/sucrose diet (HFS) on satiety hormone and serum metabolite response in disparate diets. Wistar rat pups were fed control (C), high prebiotic fiber (HF) or high protein (HP) diets (weaning to 16 weeks), HFS diet challenged (6 weeks), and finally reverted to their respective C, HF, or HP diet (4 weeks). At conclusion, measurement of body composition and satiety hormones was accompanied by (1)H NMR metabolic profiles in fasted and postprandial states. Metabolomic profiling predicted dietary source with >90% accuracy. The HF group was characterized by lowest body weight and body fat (P<0.05) and increased satiety hormone levels (glucagon-like peptide 1 and peptide-YY). Regularized modeling confirmed that the HF diet is associated with higher gut hormone secretion that could reflect the known effects of prebiotics on gut microbiota and their fementative end products, the short chain fatty acids. Rats reared on a HF diet appear to experience fewer adverse effects from an intermittent high fat diet in adulthood when rematched to their postnatal diet. Metabolite profiles associated with the diets provide a distinct biochemical signature of their effects.
Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, T2N 4N1, Canada. reimer@ucalgary.ca
Raylene A
RA
Reimer
Alannah D
AD
Maurer
Lindsay K
LK
Eller
Megan C
MC
Hallam
Rustem
R
Shaykhutdinov
Hans J
HJ
Vogel
Aalim M
AM
Weljie
D000818:Animals; D001069:Appetite Regulation; D019540:Area Under Curve; D001786:Blood Glucose; D001823:Body Composition; D003106:Colon / Q000378:metabolism; D059305:Diet, High-Fat; D004043:Dietary Fiber / Q000008:administration & dosage*; D004044:Dietary Proteins / Q000008:administration & dosage*; D002149:Energy Intake; D005247:Feeding Behavior; D005260:Female; D015870:Gene Expression; D054439:Ghrelin / Q000097:blood; D052216:Glucagon-Like Peptide 1 / Q000097:blood / Q000235:genetics / Q000378:metabolism; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D058228:Islet Amyloid Polypeptide / Q000097:blood; D020738:Leptin / Q000097:blood; D008297:Male; D055442:Metabolome; D019894:Peptide YY / Q000097:blood / Q000235:genetics / Q000378:metabolism; D056692:Prebiotics; D051381:Rats; D017208:Rats, Wistar; D012528:Satiety Response
D001786:Blood Glucose; D004043:Dietary Fiber; D004044:Dietary Proteins; D054439:Ghrelin; D007328:Insulin; D058228:Islet Amyloid Polypeptide; D020738:Leptin; D056692:Prebiotics; D019894:Peptide YY; D052216:Glucagon-Like Peptide 1