Development of a novel model of hypertriglyceridemic acute pancreatitis in hamsters: protective effects of probucol.
OBJECTIVE
The aim of the present study was to develop a model of hypertriglyceridemic (HTG) acute pancreatitis and to investigate the effects of probucol in this model.


METHODS
Hamsters were fed a high-fat diet (HFD) or a normal diet for 3 weeks. Probucol was added at 1% to the HFD in the treated group. Pancreatitis was induced by 7 peritoneal injections of cerulein to the normal and HFD hamster groups. The severity of the pancreatitis and whole body oxidative stress were assessed.


RESULTS
The HFD induced severe HTG (>1000 mg/dL) in the hamsters. A more severe pancreatitis was observed in the HFD group. The HFD did not influence plasma-reduced glutathione level, but there was a significant increase after 1% probucol was provided in the diet. Plasma malonaldehyde levels in the HFD group were significantly higher than the normal chow group, whereas probucol administration significantly decreased plasma hydrogen peroxide and malonaldehyde levels. We also found that probucol significantly reduced levels of amylase and lipase in the plasma and pathological scores in pancreatic tissue.


CONCLUSIONS
This study presents a novel model of severe HTG acute pancreatitis, and our results support the potential therapeutic application of probucol in HTG acute pancreatitis.
Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, PR China.
Guoyong
G
Hu
Yan
Y
Zhao
Yin
Y
Tang
Yuhui
Y
Wang
Jie
J
Shen
Lu
L
Liu
Haibo
H
Li
Yahan
Y
Liu
Xin
X
Cui
Yongchun
Y
Yu
Chuanyong
C
Guo
Xingpeng
X
Wang
George
G
Liu
D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D002108:Ceruletide; D006224:Cricetinae; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005978:Glutathione / Q000097:blood; D006861:Hydrogen Peroxide / Q000097:blood; D015228:Hypertriglyceridemia / Q000097:blood / Q000150:complications / Q000188:drug therapy* / Q000209:etiology; D008049:Lipase / Q000097:blood; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D010195:Pancreatitis / Q000097:blood / Q000139:chemically induced / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D011341:Probucol / Q000494:pharmacology*; D012720:Severity of Illness Index
D000975:Antioxidants; D015415:Biomarkers; D008315:Malondialdehyde; D002108:Ceruletide; D006861:Hydrogen Peroxide; D008049:Lipase; D000681:Amylases; D005978:Glutathione; D011341:Probucol