Soluble expression of OmpA from Haemophilus parasuis in Escherichia coli and its protective effects in the mouse model of infection.
Haemophilus parasuis causes contagious porcine Glässer's disease leading to severe losses in the swine industry. In this study, we established an efficient Escherichia colibased system for the expression of H. parasuis major outer-membrane protein (MOMP) that has been known as a good vaccine candidate against Glässer's disease. Use of an E. coli-derived pelB leader sequence made it possible to produce recombinant MOMP (rMOMP) as the soluble forms without an additional refolding process. Using two different animal models, it was evaluated that the rMOMP was capable of inducing a significant immune response and providing protection against H. parasuis infection.
Biotechnology Process Engineering Center, KRIBB, Daejeon 305-600, Korea.
Jungoh
J
Ahn
Minhee
M
Hong
Sungsik
S
Yoo
Eungyo
E
Lee
Hokeun
H
Won
Injoong
I
Yoon
Joon-ki
JK
Jung
Hongweon
H
Lee
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006168:Guinea Pigs; D006192:Haemophilus Infections / Q000276:immunology / Q000517:prevention & control*; D018073:Haemophilus Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D044137:Haemophilus parasuis / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D012995:Solubility; D016019:Survival Analysis
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018073:Haemophilus Vaccines; D011994:Recombinant Proteins; C078167:OMPA outer membrane proteins