A rapid method to increase the number of F₁ plants in pea (Pisum sativum) breeding programs.
In breeding programs, a large number of F₂ individuals are required to perform the selection process properly, but often few such plants are available. In order to obtain more F₂ seeds, it is necessary to multiply the F₁ plants. We developed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting of seeds using 6-benzylaminopurine. To optimize shoot regeneration, basic medium contained Murashige and Skoog (MS) salts with or without B5 Gamborg vitamins and different concentrations of 6-benzylaminopurine (25, 50 and 75 μM) using five genotypes. We found that modified MS (B5 vitamins + 25 μM 6-benzylaminopurine) is suitable for in vitro shoot regeneration of pea. Thirty-eight hybrid combinations were transferred onto selected medium to produce shoots that were used for root induction on MS medium supplemented with α-naphthalene-acetic acid. Elongated shoots were developed from all hybrid genotypes. This procedure can be used in pea breeding programs and will allow working with a large number of plants even when the F₁ plants produce few seeds.
Consejo Nacional de Investigaciones Científicas y Técnicas, Zavalla, Argentina. mesposi@unr.edu.ar
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D001947:Breeding / Q000379:methods*; D003433:Crosses, Genetic; D006824:Hybridization, Genetic; D018532:Pisum sativum / Q000254:growth & development*; D010802:Phylogeny; D018520:Plant Shoots / Q000254:growth & development / Q000502:physiology; D012038:Regeneration