Gαo and Gαq regulate the expression of daf-7, a TGFβ-like gene, in Caenorhabditis elegans.
Caenorhabditis elegans enter an alternate developmental stage called dauer in unfavorable conditions such as starvation, overcrowding, or high temperature. Several evolutionarily conserved signaling pathways control dauer formation. DAF-7/TGFβ and serotonin, important ligands in these signaling pathways, affect not only dauer formation, but also the expression of one another. The heterotrimeric G proteins GOA-1 (Gα(o)) and EGL-30 (Gα(q)) mediate serotonin signaling as well as serotonin biosynthesis in C. elegans. It is not known whether GOA-1 or EGL-30 also affect dauer formation and/or daf-7 expression, which are both modulated in part by serotonin. The purpose of this study is to better understand the relationship between proteins important for neuronal signaling and developmental plasticity in both C. elegans and humans. Using promoter-GFP transgenic worms, it was determined that both goa-1 and egl-30 regulate daf-7 expression during larval development. In addition, the normal daf-7 response to high temperature or starvation was altered in goa-1 and egl-30 mutants. Despite the effect of goa-1 and egl-30 mutations on daf-7 expression in various environmental conditions, there was no effect of the mutations on dauer formation. This paper provides evidence that while goa-1 and egl-30 are important for normal daf-7 expression, mutations in these genes are not sufficient to disrupt dauer formation.
Department of Biological and Allied Health Sciences, Fairleigh Dickinson University, College at Florham, Madison, New Jersey, United States of America. emyers@fdu.edu
Edith M
EM
Myers
D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism*; D048430:Cell Shape; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000235:genetics / Q000378:metabolism*; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D017238:Genes, Helminth / Q000235:genetics*; D007814:Larva / Q000235:genetics; D009154:Mutation / Q000235:genetics; D013217:Starvation / Q000235:genetics; D013696:Temperature; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; C102095:DAF-7 protein, C elegans; C479565:Egl-30 protein, C elegans; C516620:GOA-1 protein, C elegans; D016212:Transforming Growth Factor beta; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D043802:GTP-Binding Protein alpha Subunits, Gq-G11