Co-induction of a glutathione-S-transferase, a glutathione transporter and an ABC transporter in maize by xenobiotics.
Glutathione conjugation reactions are one of the principal mechanisms that plants utilize to detoxify xenobiotics. The induction by four herbicides (2,4-D, atrazine, metolachlor and primisulfuron) and a herbicide safener (dichlormid) on the expression of three genes, ZmGST27, ZmGT1 and ZmMRP1, encoding respectively a glutathione-S-transferase, a glutathione transporter and an ATP-binding cassette (ABC) transporter was studied in maize. The results demonstrate that the inducing effect on gene expression varies with both chemicals and genes. The expression of ZmGST27 and ZmMRP1 was up-regulated by all five compounds, whereas that of ZmGT1 was increased by atrazine, metolachlor, primisulfuron and dichlormid, but not by 2,4-D. For all chemicals, the inducing effect was first detected on ZmGST27. The finding that ZmGT1 is activated alongside ZmGST27 and ZmMRP1 suggests that glutathione transporters are an important component in the xenobiotic detoxification system of plants.
Engineering Research Center of Plant Growth Regulators, Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China.
Sen
S
Pang
Liusheng
L
Duan
Zhiqian
Z
Liu
Xiaoyu
X
Song
Xuefeng
X
Li
Chengju
C
Wang
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D006540:Herbicides / Q000378:metabolism / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D015262:Xenobiotics / Q000378:metabolism* / Q000494:pharmacology; D003313:Zea mays / Q000235:genetics / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D006540:Herbicides; D026901:Membrane Transport Proteins; D015262:Xenobiotics; C086860:glutathione transporter; D005982:Glutathione Transferase