Fully automated sequential solid phase approach towards viral RNA-nucleopeptides.
The synthesis of two ribonucleoprotein fragments of unprecedented complexity is reported. These hybrid biomolecules are prepared combining the use of an automated solid phase peptide and oligonucleotide synthesizer on a single solid support.
Leiden Institute of Chemistry, Leiden University, P. O. Box 9502, 2300 RA Leiden, The Netherlands.
Gerbrand J
GJ
van der Heden van Noort
Pieter
P
van Delft
Nico J
NJ
Meeuwenoord
Herman S
HS
Overkleeft
Gijsbert A
GA
van der Marel
Dmitri V
DV
Filippov
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D017955:Poliovirus / Q000737:chemistry*; D012367:RNA, Viral / Q000138:chemical synthesis / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000138:chemical synthesis* / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*; D014764:Viral Proteins / Q000138:chemical synthesis* / Q000737:chemistry
D009841:Oligonucleotides; D010455:Peptides; D012367:RNA, Viral; D012261:Ribonucleoproteins; D014764:Viral Proteins