[Microbial community in nitrogen cycle of aquaculture water of the Pearl River Delta].
OBJECTIVE
In order to study the characteristic of nitrogen transport, the community structure and diversity of related microorganisms in aquaculture water of the Pearl River Delta.


METHODS
We established an artificial aquaculture ecosystem to study the microbial community of 15N-stable isotope probing (15N-SIP) labeled nitrogen transport microorganisms. The 15N-labeled DNA was separated by CsCl-ethidium bromide density gradient centrifugation, and was used to construct 16S rRNA gene clone libraries of bacteria and archaea.


RESULTS
Phylogenetic analysis shows that 19 Operational Taxonomic Units (OTUs) from bacterial library were clustered in Proteobacteria and Planctomycetes. Proteobacteria (99.2%) was the dominant group, mainly consisted of Comamonas (15.7%), Nitrosomonas (12.4%), Enterobacteriaceae (11.5%) and Nitrobacter (11.5%). From archaeal library 9 OTUs were divided into 3 phyla: Thaumarchaeota, Crenarchaeota and Euryarchaeota.


CONCLUSIONS
We successfully elucidated the microbial community of nitrogen transport microorganisms in aquaculture water of Pearl River Delta by using 15N-SIP. The data of the community will provide essential information for isolating nitrogen degrading microorganism, and provide scientific basis for creating a healthy aquaculture environment.
School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, China. alancai2011@163.com
Xiaolong
X
Cai
Jianfei
J
Luo
Weitie
W
Lin
Guoliang
G
Tian
D017756:Aquaculture; D001105:Archaea / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002681:China; D017753:Ecosystem; D009584:Nitrogen / Q000378:metabolism*; D058458:Nitrogen Cycle; D010802:Phylogeny; D045483:Rivers / Q000382:microbiology*
D009584:Nitrogen