Targeting both rs12979860 and rs8099917 polymorphisms with a single-tube high-resolution melting assay for IL28b genotyping.
A rapid, duplex, high-resolution melting interleukin-28B gene (IL28B) genotyping assay, targeting both rs12979860 and rs8099917 polymorphisms, was developed and validated using 30 DNA samples from healthy volunteers. A linkage study on 300 healthy Singaporeans showed variable haplotypes. When the assay was applied to plasma DNA from 50 hepatitis C virus genotype-1 (HCV-1)-infected patients, five compound heterozygous types were detected.
Molecular Diagnosis Centre, Department of Laboratory Medicine, National University Health System, Singapore.
Kok Siong
KS
Poon
Sherry Sze Yee
SS
Ho
Julian Wei-Tze
JW
Tang
Cui Wen
CW
Chua
Lily
L
Chiu
Evelyn Siew-Chuan
ES
Koay
D019411:Clinical Laboratory Techniques / Q000379:methods*; D005838:Genotype; D006239:Haplotypes; D016174:Hepacivirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006526:Hepatitis C / Q000188:drug therapy* / Q000821:virology; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009691:Nucleic Acid Denaturation; D020641:Polymorphism, Single Nucleotide; D012846:Singapore; D044366:Transition Temperature; D000095022:Interferon Lambda
D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda