[Influence of combination on the specific chromatogram of Glycyrrhiza in sini decoctions by HPLC].
The paper is to report the establishment of an HPLC specific chromatogram of Glycyrrhiza in Sini decoctions and the influence of combination on the specific chromatogram. The RP-HPLC method was used with a Phenomenex Gemini C18 column (250 mm x 4.6 mm ID, 5 microm), and acetonitrile-0.05% trifluoroacetic acid (gradient elution) as mobile phase. Flow rate was 0.8 mL x min(-1) and the detection wavelength was set at 232 nm. The temperature of column was 30 degrees C. The method is stable and reliable with a good reproducibility, it can be used to determine the specific chromatogram of Glycyrrhiza in Sini Decoctions. Twenty peaks were selected as specific peaks in Sini Decoction with liquiritin peak as the reference peak. Six of them were from Glycyrrhiza and the other 6 peaks were from both Glycyrrhiza and Ganjiangfuzi Decoction. The areas of specific peaks of Sini Decoctions were smaller than those in the chromatogram of Glycyrrhiza. The specific chromatogram of Glycyrrhiza in Sini Decoctions is markedly influenced by Radix Aconiti Carmichaeli and Rhizoma Zingiberis. The areas of the specific peaks in Sini Decoctions were reduced obviously. The method is stable and reliable with a good reproducibility, it can be used to determine the specific chromatogram of Glycyrrhiza in Sini Decoctions.
Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine, Ministry of Education, Shanghai 201203, China.
Huai-Bin
HB
Zhao
Yan-Long
YL
Hong
You-Jie
YJ
Wang
Lan
L
Shen
Fei
F
Wu
Yi
Y
Feng
Ke-Feng
KF
Ruan
D031841:Aconitum / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D044950:Flavanones / Q000737:chemistry; D020939:Zingiber officinale / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry; D006035:Glycyrrhiza / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; C478756:sini tang; C512196:liquiritin