PCR-based gene marker with a high efficiency genome scanning (HEGS) system: application for distinguishing among cultivars in Lentinula edodes.
To establish a high throughput and cost-efficiency procedure for distinguishing among cultivars in Lentinula edodes, the polymorphism in the genes reported previously in this fungus was examined using PCR or PCR-RFLP techniques with a high-efficiency genome scanning (HEGS) system. As a result, PCR-based markers derived from eight genes (tyr, cap, ppa, IGS-RFLP, pri B-RFLP, mfbC-RFLP, gla-RFLP, xy-RFLP) showed polymorphisms among cultivars in this fungus and consequently, enabled to distinguish seventy-nine cultivars used in this study.
Tottori Mycological Institute, Tottori 689-1125, Japan. kin-ters@infosakyu.ne.jp
Kazuhisa
K
Terashima
Chisato
C
Funato
Teruyuki
T
Matsumoto
Asa
A
Maeda
Kozaburo
K
Hasebe
D005819:Genetic Markers; D016681:Genome, Fungal; D058889:Molecular Typing / Q000379:methods*; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020942:Shiitake Mushrooms / Q000145:classification* / Q000235:genetics*
D005819:Genetic Markers