StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst.
• Potato (Solanum tuberosum) calcium-dependent protein kinase (StCDPK5) has been shown to phosphorylate the N-terminal region of plasma membrane RBOH (respiratory burst oxidase homolog) proteins, and participate in StRBOHB-mediated reactive oxygen species (ROS) burst. The constitutively active form, StCDPK5VK, provides a useful tool for gain-of-function analysis of RBOH in defense responses. • StCDPK5- and StCDPK5VK-green fluorescent protein fusion proteins were predominantly targeted to the plasma membrane, and conditional expression of StCDPK5VK activated StRBOHA-D. The interaction was confirmed by bimolecular fluorescence complementation assay. We generated transgenic potato plants containing StCDPK5VK under the control of a pathogen-inducible promoter to investigate the role of ROS burst on defense responses to blight pathogens. • Virulent isolates of the late blight pathogen Phytophthora infestans and the early blight pathogen Alternaria solani induced hypersensitive response-like cell death accompanied by ROS production at the infection sites of transgenic plants. Transgenic plants showed resistance to the near-obligate hemibiotrophic pathogen P. infestans and, by contrast, increased susceptibility to the necrotrophic pathogen A. solani. • These results indicate that RBOH-dependent ROS contribute to basal defense against near-obligate pathogens, but have a negative role in resistance or have a positive role in expansion of disease lesions caused by necrotrophic pathogens.
Michie
M
Kobayashi
Miki
M
Yoshioka
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Shuta
S
Asai
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Hironari
H
Nomura
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Kazuo
K
Kuchimura
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Hitoshi
H
Mori
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Noriyuki
N
Doke
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Hirofumi
H
Yoshioka
D000528:Alternaria / Q000502:physiology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics / Q000276:immunology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase; D008954:Models, Biological; D008969:Molecular Sequence Data; D055750:Phytophthora infestans / Q000472:pathogenicity / Q000502:physiology*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D021381:Protein Transport; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016897:Respiratory Burst / Q000235:genetics; D011198:Solanum tuberosum / Q000201:enzymology* / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D005966:Glucuronidase; D010940:Plant Proteins; D011494:Protein Kinases; D017382:Reactive Oxygen Species; D017871:Calcium-Calmodulin-Dependent Protein Kinases