The use of layer by layer self-assembled coatings of hyaluronic acid and cationized gelatin to improve the biocompatibility of poly(ethylene terephthalate) artificial ligaments for reconstruction of the anterior cruciate ligament.
In this study layer by layer (LBL) self-assembled coatings of hyaluronic acid (HA) and cationized gelatin (CG) were used to modify polyethylene terephthalate (PET) artificial ligament grafts. Changes in the surface properties were characterized by scanning electron microscopy, attenuated total reflection Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, and contact angle and biomechanical measurements. The cell compatibility of this HA-CG coating was investigated in vitro on PET films seeded with human foreskin dermal fibroblasts over 7days. The results of our in vitro studies demonstrated that the HA-CG coating significantly enhanced cell adhesion, facilitated cell growth, and suppressed the expression of inflammation-related genes relative to a pure PET graft. Furthermore, rabbit and porcine anterior cruciate ligament reconstruction models were used to evaluate the effect of this LBL coating in vivo. The animal experiment results proved that this LBL coating significantly inhibited inflammatory cell infiltration and promoted new ligament tissue regeneration among the graft fibers. In addition, the formation of type I collagen in the HA-CG coating group was much higher than in the control group. Based on these results we conclude that PET grafts coated with HA-CG have considerable potential as substitutes for ligament reconstruction.
Department of Sports Medicine, Huashan Hospital, Shanghai, People's Republic of China.
Hong
H
Li
Chen
C
Chen
Shurong
S
Zhang
Jia
J
Jiang
Hongyue
H
Tao
Jialing
J
Xu
Jianguo
J
Sun
Wei
W
Zhong
Shiyi
S
Chen
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000473:pathology / Q000601:surgery*; D002412:Cations; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000648:ultrastructure; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D056951:Photoelectron Spectroscopy; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D011817:Rabbits; D019651:Plastic Surgery Procedures; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013499:Surface Properties; D034421:Sus scrofa
D002412:Cations; D020099:Coated Materials, Biocompatible; D011093:Polyethylene Terephthalates; D005780:Gelatin; D006820:Hyaluronic Acid