[Construction and characterization of aroA deletion mutant of Pasteurella multocida strain C51-17].
OBJECTIVE
We constructed Pasteurella multocida strain C51-17 aroA mutant and characterized its virulence.


METHODS
Suicide recombinant plasmid of pBC-aroA-Tn903 was constructed with kanamycin gene and transformed into Pasteurella multocida by electroporation. aroA mutant strain of P. multocida was screened by kanamycin resistance and chloromycetin sensitivity and identified by PCR. Virulence of aroA mutant strain was tested in mice by intraperitoneal injection.


RESULTS
The aroA mutant strain of P. multocida C51-17 was successfully constructed. Growth rate of aroA mutant strain was slower within the first 6 hour and similar to the wild-type strain in vitro. The virulence test indicated that aroA mutant strain was significant attenuated and no death was found in mice even inoculated at a dosage of 1.0 x 10(6) CFU.


CONCLUSIONS
The virulence of aroA mutant strain of P. multocida was highly attenuated in mice. The results of this study will contribute to a better understanding of pathogenesis of P. multocida.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin 150001, China. gdongchun@hvri.ac.cn
Dongchun
D
Guo
Yan
Y
Lu
Jiasen
J
Liu
Dongwei
D
Yuan
Aiqin
A
Zhang
Qian
Q
Jiang
Huan
H
Lin
Changde
C
Si
Liandong
L
Qu
D000818:Animals; D017353:Gene Deletion; D005798:Genes, Bacterial; D051379:Mice; D008813:Mice, Inbred ICR; D016979:Pasteurella multocida / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence