Molecular and immunohistochemical diagnosis of Francisella noatunensis subsp. orientalis from formalin-fixed, paraffin-embedded tissues.
Members of the genus Francisella (viz., F. noatunensis subsp. orientalis [Fno] and F. noatunensis subsp. noatunensis) have been described as causative agents of chronic granulomatous and pyogranulomatous lesions in wild and cultured fish species. In the present study, 68 archived formalin-fixed, paraffin-embedded (FFPE) tissues from several fish species, collected at different geographical locations from 2000 to 2011, were analyzed using a real-time polymerase chain reaction assay for the detection of the Fno intracellular growth loci C (iglC) gene and by immunohistochemistry for the demonstration of Fno antigens. The results revealed a high correlation between these 2 diagnostic techniques validating their use for the diagnosis of Fno infection in archived FFPE tissues and confirming the presence of Fno in fish species from the Cari y years of the present century.
Ross University, School of Veterinary Medicine, Department of Pathobiology, PO Box 334, Basseterre, St. Kitts, West Indies. esoto@rossvet.edu.kn
Esteban
E
Soto
Oscar
O
Illanes
David
D
Hilchie
Juan A
JA
Morales
Piyanate
P
Sunyakumthorn
John P
JP
Hawke
Andrew E
AE
Goodwin
Allen
A
Riggs
Roy P
RP
Yanong
Deborah B
DB
Pouder
Ruth
R
Francis-Floyd
Maziel
M
Arauz
Lewis
L
Bogdanovic
Fernanda
F
Castillo-Alcala
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000453:epidemiology / Q000382:microbiology; D005399:Fishes / Q000382:microbiology*; D005557:Formaldehyde; D005603:Francisella / Q000302:isolation & purification*; D014943:Global Health; D007150:Immunohistochemistry; D016612:Paraffin Embedding; D015203:Reproducibility of Results; D013045:Species Specificity; D016707:Tissue Fixation / Q000379:methods / Q000662:veterinary*
D001426:Bacterial Proteins; D005557:Formaldehyde