The effect of Hg2+ on the bioluminescence of Photobacterium leiognathi.
Photobacteria were used as test objects for rapid monitoring of ecotoxicants. Specific inhibitory effects of Hg(2+) on bioluminescence and cell growth as well as the lux gene expression of Photobacterium leiognathi were studied. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay was used to evaluate cellular proliferation and mortality. The luminescent inhibition effect on P. leiognathi cells was found to increase as cellular mortality increased; y = 0.744x - 4.8916, R² = 0.9794. However, this trend was not observed in cell growth processes. Quantitation of lux mRNAs by semi-quantitative RT-qPCR indicated that increases and decreases in luciferase mRNA integral level coincided with changes in luminescence intensity (R(2) = 0.93). Addition of Hg(2+) changed luminescence but without concomitant changes in extractable luciferase activity. Nevertheless, the presence of Hg(2+) changed lux gene expression. This phenomenon requires further research.
College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
Ming
M
Li
Jingxue
J
Wang
Hong
H
Lin
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D008156:Luciferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D049449:Luminescence; D008163:Luminescent Measurements; D008628:Mercury / Q000494:pharmacology*; D010776:Photobacterium / Q000737:chemistry* / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics
D001426:Bacterial Proteins; D008156:Luciferases; D008628:Mercury