Domestic microwave processing for rapid immunohistochemical diagnosis of bovine rabies.
The present study describes the use of a microwave processing protocol for the rapid histopathological and immunohistochemical diagnosis of bovine rabies. Immunohistochemistry has been used for rabies diagnosis in formalin-fixed tissue with satisfactory results, although the time to diagnosis is considerably longer than that with direct immunofluorescence. The protocol provided a provisory histopathological rabies diagnosis in approximately three and half hours and the immunohistochemical diagnosis was available after six hours. The protocol achieved 100% correlation with direct immunofluorescence and is a promising method, particularly in situations in which only material in formalin is available for diagnosis or when the refrigeration or transportation of biological material is difficult.
Veterinary Pathology, Department of Veterinary Medicine, Federal University of Lavras, Lavras, MG, Brazil.
Camila C
CC
Abreu
Priscilla A
PA
Nakayama
Clayton I
CI
Nogueira
Leonardo P
LP
Mesquita
Priscila F R
PF
Lopes
Mary S
MS
Varaschin
Josilene do N
Jdo N
Seixas
Enio
E
Ferreira
Pedro S
PS
Bezerra
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D016591:Histocytological Preparation Techniques / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods; D008872:Microwaves; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary*