Benzene removal by a novel modification of enhanced anaerobic biostimulation.
A novel modification of enhanced anaerobic bioremediation techniques was developed by using non-activated persulfate to accelerate the organic phosphorus breakdown and then stimulate benzene biodegradation by nitrate and sulfate reduction. Benzene concentrations in groundwater where nitrate, triethyl phosphate and persulfate were successfully injected were reduced at removal efficiencies greater than 77% to the levels below the applicable guideline. Soil benzene was removed effectively by the modification of the enhanced anaerobic bioremediation with removal efficiencies ranging between 75.9% and 92.8%. Geochemical analytical results indicated that persulfate effectively breaks down triethyl phosphate into orthophosphate, thereby promoting nitrate and sulfate utilization. Microbial analyses (quantitative polymerase chain reaction, denaturing gradient gel electrophoresis and 16S ribosomal RNA) demonstrated that benzene was primarily biodegraded by nitrate reduction while sulfate reduction played an important role in benzene removal at some portions of the study site. Enrichment in the heavier carbon isotope ¹³C of residual benzene with the increased removal efficiency provided direct evidence for benzene biodegradation. Nitrogen, sulfur and oxygen isotope analyses indicated that both nitrate reduction and sulfate reduction were occurring as bioremediation mechanisms.
Stantec Consulting Ltd., Saskatoon, Saskatchewan S7K 0K3, Canada. wex167@gmail.com
Wenhui
W
Xiong
Chris
C
Mathies
Kris
K
Bradshaw
Trevor
T
Carlson
Kimberley
K
Tang
Yi
Y
Wang
D000693:Anaerobiosis; D001554:Benzene / Q000302:isolation & purification*; D001673:Biodegradation, Environmental; D004591:Electrophoresis, Polyacrylamide Gel; D060587:Groundwater / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012989:Soil Pollutants / Q000302:isolation & purification*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D001554:Benzene