Biliopancreatic diversion induces villi elongation and cholecystokinin and ghrelin increase.
BACKGROUND
Factors leading to weight loss and weight stabilization after bariatric surgery are not fully understood. Our aim was to evaluate, in Sprague-Dawley rats, the histological and gut hormonal changes after Larrad-biliopancreatic diversion (Larrad-BPD).


METHODS
Rats randomly underwent the following protocols: Larrad-BPD (n=4) versus pair fed (PF) (n=4). Weight and food intake were measured every day. By immunohistochemistry ghrelin was examined in the stomach, while cholecystokinin (CCK), glucagon-like-peptide-1 (GLP-1), peptide YY (PYY) and serotonin (5-HT) expression were analyzed in alimentary limb and ileum following or not the Larrad-BPD.


RESULTS
Larrad-BPD rats exhibited significant (P<0.05) weight loss compared to PF rats. Villi enlongation was observed in Larrad-BPD rats. In residual stomach, ghrelin was diminished. In the alimentary limb, ghrelin and CCK positive cells were detected more than in the ileum of PF rats. GLP-1 expression was decreased and PYY expression was absent after Larrad-BPD compared with PF rats.


CONCLUSIONS
Larrad-BPD is followed by histological changes and a pleiotropic gut endocrine response aimed to compensate the reduction of intestinal area exposed to food. Until now, the hormones responsible for the intestinal hypertrophy have not been defined.
Medical Research Center (CICMED), Autonomous University of the State of Mexico (UAEMEx), Toluca, Mexico. mezh_74@yahoo.com
Hugo
H
Mendieta-Zerón
Álvaro
Á
Larrad-Jiménez
María Angela
MA
Burrell
Marina Martín
MM
Rodríguez
Katia
K
Da Boit
Gema
G
Frühbeck
Carlos
C
Diéguez
D000818:Animals; D015904:Biliopancreatic Diversion / Q000379:methods / Q000639:trends*; D001835:Body Weight / Q000502:physiology; D002766:Cholecystokinin / Q000096:biosynthesis*; D002149:Energy Intake / Q000502:physiology; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism* / Q000503:physiopathology; D054439:Ghrelin / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D054439:Ghrelin; D002766:Cholecystokinin