[Determination of tobacco-specific N'-nitrosamines in mainstream smoke from Japanese cigarettes].
OBJECTIVE
Mainstream smoke from cigarettes contains tobacco-specific N'-nitrosamines (TSNAs) listed as Group 1 and 3 carcinogens by the International Agency for Research on Cancer (IARC). Herein, we report on a method of measuring the concentrations of TSNAs in mainstream smoke from the ten top-selling Japanese cigarette brands using an ISO regime by International Organization for Standardization (ISO) and HCI regime of Health Canada.


METHODS
Tar in mainstream smoke was collected on a Cambridge filter pad using a smoking machine. The filter pad was immersed in 40 mL of ammonium acetate (pH 6.8) and shaken for 30 min. The extract was then loaded into a C18 column. After washing with 5 mL of 10% methanol and eluting with 5 mL of 70% methanol, the eluate was concentrated to 1 mL for LC-MS/MS analysis.


RESULTS
The concentrations of TSNAs in all cigarette brands were higher when determined using the HCI regime than when determined using the ISO regime. Furthermore, the concentrations of TSNAs measured using both the ISO and HCI regimes showed negligible correlation to the tar and nicotine concentrations indicated on package labels. The cigarette samples used in the study were categorized into four classes: ultralow-, low-, medium-, and high-yield brands, which corresponded to 1, 3-6, 8-10, and 14 mg tar/cigarette, respectively. The concentration of TSNAs in ultralow-yield cigarettes was 210 ng/cigarette, as measured using the HCI regime, which was nearly equal to that in high-yield cigarettes (180 ng/cigarette).


CONCLUSIONS
Exposure to TSNAs from mainstream smoke from ultralow-yield cigarettes is comparable to that from high-yield cigarettes. To properly evaluate the risk of smoking, not only the concentrations of tar and nicotine but also those of other chemicals, including TSNAs, should be printed on package labels.
Department of Veterinary Medicine, Azabu University, Japan.
Koichi
K
Sugiyama
Yohei
Y
Inaba
Tadamichi
T
Ohkubo
Shigehisa
S
Uchiyama
Yukihiko
Y
Takagi
Naoki
N
Kunugita
D001060:Aporphines / Q000032:analysis*; D002273:Carcinogens / Q000032:analysis; D002853:Chromatography, Liquid; D007564:Japan; D015203:Reproducibility of Results; D012906:Smoke / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014026:Nicotiana
D001060:Aporphines; D002273:Carcinogens; C513721:N-nitrosoanonaine; D012906:Smoke