Development of a rapid immunochromatographic test using a recombinant thrombospondin-related adhesive protein of Babesia gibsoni.
We developed an immunochromatographic test (ICT) with the full-length of thrombospondin-related adhesive protein of Babesia gibsoni expressed by the modified expression method. The developed ICT showed high sensitivity, specificity, and kappa value with a reference test (100%, 93.78%, and 0.8976, respectively), indicating that the ICT could be a new practical diagnostic test for B. gibsoni infection.
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Youn-Kyoung
YK
Goo
Naeun
N
Lee
Mohamad Alaa
MA
Terkawi
Yuzi
Y
Luo
Gabriel Oluga
GO
Aboge
Yoshifumi
Y
Nishikawa
Hiroshi
H
Suzuki
Suk
S
Kim
Xuenan
X
Xuan
D000818:Animals; D001403:Babesia / Q000378:metabolism*; D001404:Babesiosis / Q000175:diagnosis*; D002448:Cell Adhesion; D002846:Chromatography, Affinity / Q000379:methods / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis / Q000469:parasitology; D004285:Dogs; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism*; D012680:Sensitivity and Specificity; D019699:Thrombospondins / Q000737:chemistry / Q000378:metabolism*
D015800:Protozoan Proteins; D011994:Recombinant Proteins; D019699:Thrombospondins