Effects of Azospirillum brasilense Sp7 lectin and Phaseolus vulgarus phytohemagglutinin on cytokine activity of lymphocytes in vitro.
We studied the effect of fucose-specific lectin from Azospirillum brasilense Sp7 bacteria and Phaseolus vulgarus phytohemagglutinin (PHA) on activity of IFN-γ, IFN-α, IL-1α, IL-4, IL-8, and TNF-α in human blood in vitro. During the experiment, IFN-γ production increased from 25 pg/ml in controls to 103 and 56 pg/ml under the influence of lectin and PHA, respectively. Agglutinins had similar effects on IFN-α production. Bacterial lectin increased IL-1α level from 2.65±0.08 to 8.45±0.41 pg/ml. PHA also increased IL-1α levels, but this effect was by 1.5-2 times less pronounced. Bacterial lectin and PHA increased production IL-4 by almost 2 times and production of IL-8 by 6 and 5 times, respectively, compared to the control. No differences were found in the effects of bacterial lectin and PHA on TNF-α synthesis. Experiments showed that immunostimulatory and immunomodulatory effects of bacterial lectin are more pronounced than those of PHA.
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia. ponomareva@ibppm.sgu.ru
E G
EG
Ponomareva
N V
NV
Emelyanova
V E
VE
Nikitina
D015806:Azospirillum brasilense / Q000737:chemistry*; D002499:Centrifugation, Density Gradient; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D053582:Interleukin-1alpha / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D027805:Phaseolus / Q000737:chemistry*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D016898:Interferon-alpha; D053582:Interleukin-1alpha; D016209:Interleukin-8; D037102:Lectins; D010835:Phytohemagglutinins; D014409:Tumor Necrosis Factor-alpha; C064549:lectin, Aleuria aurantia; D015847:Interleukin-4; D007371:Interferon-gamma