Effect of insulin-like growth factor-1 on bone morphogenetic protein-2 expression in hepatic carcinoma SMMC7721 cells through the p38 MAPK signaling pathway.
OBJECTIVE
To observe the effect of insulin-like growth factor-1 (IGF-1) on bone morphogenetic protein (BMP)-2 expression in hepatocellular carcinoma SMMC7721 cells.


METHODS
Cells were divided into blank control, IGF-1, IGF-1 + SB203580, and SB203580 groups. SB203580 was used to block the p38 MAPK signaling pathway. Changes in the expression of BMP-2, p38 MAPK, and phosphorylated p38, MERK, ERK and JNK were determined using reverse transcription polymerase chain reactions (RT-PCR) and Western blot analysis.


RESULTS
Protein expression of phosphorylated BMP-2, MERK, ERK, and JNK was significantly up-regulated by IGF-1 compared with the control group (1.138 ± 0.065 vs. 0.606 ± 0.013, 0.292 ± 0.005 vs. 0.150 ± 0.081, 0.378 ± 0.006 vs. 0.606 ± 0.013, and 0.299 ± 0.015 vs. 0.196 ± 0.017, respectively; P < 0.05). Levels of BMP-2 and phosphorylated MERK and JNK were significantly reduced after blocking of the p38MAPK signaling pathway (0.494 ± 0.052 vs. 0.165 ± 0.017, 0.073 ± 0.07 vs. 0.150 ± 0.081, and 0.018 ± 0.008 vs. 0.196 ± 0.017, respectively; P <0.05), but such a significant difference was not observed for phosphorylated ERK protein expression (0.173 ∓ 0.07 vs. 0.150 ∓ 0.081, P > 0.05).


CONCLUSIONS
IGF-1 can up-regulate BMP-2 expression, and p38 MAPK signaling pathway blockage can noticeably reduce the up-regulated expression. We can conclude that the up-regulatory effect of IGF-1 on BMP-2 expression is realized through the p38 MAPK signaling pathway.
Department of Oncology, the Second Affiliated Hospital of Nanchang University, Nanchang, China.
Guan-Jun
GJ
Xu
Sheng
S
Cai
Jian-Bing
JB
Wu
D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma / Q000201:enzymology / Q000378:metabolism*; D045744:Cell Line, Tumor; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D048031:JNK Mitogen-Activated Protein Kinases / Q000187:drug effects / Q000378:metabolism; D008113:Liver Neoplasms / Q000201:enzymology / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D010766:Phosphorylation / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000187:drug effects / Q000378:metabolism
D055396:Bone Morphogenetic Protein 2; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases