Proliferation of Walker 256 carcinosarcoma cells: effect of whole-body hyperthermia and antitumor agents.
We studied the main biological characteristics of spontaneous growth of Walker 256 carcinosarcoma and under conditions of antitumor therapy. A combination of whole-body hyperthermia and cytostatic treatment (cyclophosphamide and melatonin) produced maximum suppression of the tumor growth, inhibition of mitotic activity of tumor cells, and stimulation of their necrotic and apoptotic death. The maximum decrease in mitotic activity of tumor cells was observed after combined exposure to whole-body hyperthermia and both cytostatic preparations; enhancement of apoptotic cell death and the decrease in the tumor node weight were also most pronounced under these conditions and practically no body weight loss was recorded in this case.
Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia. pathol@soramn.ru
E L
EL
Lushnikova
E V
EV
Ovsyanko
L M
LM
Nepomnyashchikh
A V
AV
Efremov
D V
DV
Morozov
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D017209:Apoptosis; D002279:Carcinoma 256, Walker / Q000473:pathology* / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003131:Combined Modality Therapy; D003520:Cyclophosphamide / Q000008:administration & dosage; D004354:Drug Screening Assays, Antitumor; D006979:Hyperthermia, Induced; D008550:Melatonin / Q000008:administration & dosage; D009368:Neoplasm Transplantation; D051381:Rats; D017208:Rats, Wistar; D047368:Tumor Burden / Q000187:drug effects
D003520:Cyclophosphamide; D008550:Melatonin