Evaluation of cytotoxicity of 3-mercaptopropionic acid-modified quantum dots on Medicago sativa cells and tissues.
Like most of the new technologies designed to interact with biological systems, the applications of -nanomaterials needs a proper assessment for their potential impacts. It is only through addressing the issues raised by toxicological studies that nanotechnology will be able to acquire its full potential. Here, we describe the detailed protocols to study the responses of plant cells to their exposure to nanoparticles, including viability, oxidative stress detection, and reactive oxygen species enzymatic detoxification, as well as particle uptake.
Diagnostic, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal. anaraqssantos@gmail.com
Ana Raquel
AR
Santos
Ana Sofia
AS
Miguel
Pedro
P
Fevereiro
Abel
A
Oliva
D015097:3-Mercaptopropionic Acid / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D018929:Cell Culture Techniques; D002470:Cell Survival; D000455:Medicago sativa / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity*; D018517:Plant Roots / Q000378:metabolism; D045663:Quantum Dots; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D018675:Toxicity Tests / Q000379:methods*
D017382:Reactive Oxygen Species; D015097:3-Mercaptopropionic Acid; D002374:Catalase; D013482:Superoxide Dismutase