Advances in the proteomic investigation of the cell secretome.
Studies of the cell secretome have greatly increased in recent years owing to improvements in proteomic platforms, mass spectrometry instrumentation and to the increased interaction between analytical chemists, biologists and clinicians. Several secretome studies have been implemented in different areas of research, leading to the generation of a valuable secretome catalogs. Secreted proteins continue to be an important source of biomarkers and therapeutic target discovery and are equally valuable in the field of microbiology. Several discoveries have been achieved in vitro using cell culture systems, ex vivo using human tissue specimens and in vivo using animal models. In this review, some of the most recent advances in secretome studies and the fields that have benefited the most from this evolving technology are highlighted.
Children's National Medical Center, Center for Genetic Medicine Research, 111 Michigan Avenue NW, Washington, DC 20010, USA.
Kristy J
KJ
Brown
Catherine A
CA
Formolo
Haeri
H
Seol
Ramya L
RL
Marathi
Stephanie
S
Duguez
Eunkyung
E
An
Dinesh
D
Pillai
Javad
J
Nazarian
Brian R
BR
Rood
Yetrib
Y
Hathout
D000653:Amniotic Fluid / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis; D012634:Bodily Secretions / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry; D055354:Exosomes / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009369:Neoplasms / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
D014408:Biomarkers, Tumor; D011506:Proteins