Effects of peptide Pro-Gly-Pro-Leu under conditions of normal hemostasis and thrombus formation in rats.
Tetrapeptide Pro-Gly-Pro-Leu in vitro effectively inhibited platelet aggregation over the entire range of studied concentrations (10(-12)-10(-3) M). In concentrations of 10(-9)-10(-3) M it exhibits fibrinolytic activity and in concentrations of 10(-5)-10(-3) M has anticoagulant properties. Under in vivo conditions the analyzed tetrapeptide in a dose of 1 mg/kg increased anticoagulant, total and fibrin depolymerizating activities and increased activity of plasminogen activator. Intravenous administration produced more pronounced anticoagulant effect and leads to a greater increase in activity of plasminogen activator than intranasal administration. Tetrapeptide Pro-Gly-Pro-Leu also exerts antithrombotic effect. Preliminary repeated intranasal administration of the peptide before blood clot formation reduces the weight of fresh fibrin clots.
Department of Human and Animal Physiology, Biological Faculty, M. V. Lomonosov Moscow State University, Russia.
T Y
TY
Obergan
D L
DL
Rochev
L A
LA
Lyapina
D000818:Animals; D000925:Anticoagulants / Q000627:therapeutic use*; D005342:Fibrinolysis / Q000187:drug effects; D006487:Hemostasis / Q000187:drug effects*; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology* / Q000627:therapeutic use*; D051381:Rats; D013927:Thrombosis / Q000188:drug therapy* / Q000378:metabolism*
D000925:Anticoagulants; D009842:Oligopeptides; C551701:prolyl-glycyl-prolyl-leucine