[Response surface method optimize of nano-silica solid dispersion technology assistant enzymatic hydrolysis preparation genistein].
This article reports that nano-silica solid dispersion technology was used to raise genistein efficiency through increasing the enzymatic hydrolysis rate. Firstly, genistin-nano-silica solid dispersion was prepared by solvent method. And differential scanning calorimetry (DSC) and transmission electron microscopy (TEM) were used to verify the formation of solid dispersion, then enzymatic hydrolysis of solid dispersion was done by snailase to get genistein. With the conversion of genistein as criteria, single factor experiments were used to study the different factors affecting enzymatic hydrolysis of genistin and its solid dispersion. And then, response surface method was used to optimize of nano-silica solid dispersion technology assistant enzymatic hydrolysis. The optimum condition to get genistein through enzymatic hydrolysis of genistin-nano-silica solid dispersion was pH 7.1, temperature 52.2 degrees C, enzyme concentration 5.0 mg x mL(-1) and reaction time 7 h. Under this condition, the conversion of genistein was (93.47 +/- 2.40)%. Comparing with that without forming the genistin-nano-silica solid dispersion, the conversion increased 2.62 fold. At the same time, the product of hydrolysis was purified to get pure genistein. The method of enzymatic hydrolysis of genistin-nano-silica solid dispersion by snailase to obtain genistein is simple, efficiency and suitable for the modern scale production.
Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
Xin
X
Jin
Zhen-Hai
ZH
Zhang
Jing
J
Zhu
E
E
Sun
Dan-Hong
DH
Yu
Xiao-Yun
XY
Chen
Qi-Yuan
QY
Liu
Qing
Q
Ning
Xiao-Bin
XB
Jia
D000818:Animals; D002152:Calorimetry, Differential Scanning; D019833:Genistein / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007529:Isoflavones / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D048789:Phytoestrogens / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D012908:Snails / Q000201:enzymology; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods
D007529:Isoflavones; D048789:Phytoestrogens; C040641:genistin; D012822:Silicon Dioxide; D019833:Genistein