Polysaccharide extracted from Rheum tanguticum prevents irradiation-induced immune damage in mice.
OBJECTIVE
To investigate the protective effect of purified fraction 1 polysaccharide extracted from Rheum tanguticum RTP1 on irradiation-induced immune damage in mice.


METHODS
Kunming mice were randomly divided into five groups: normal group (NC), irradiation control group (IC), RTP1 low dose (200 mg/kg), middle dose (400 mg/kg) and high dose (800 mg/kg) groups. RTP1 was administered by the gastric route for 14 d, mice in the NC and IC groups being given by 0.9% sodium chloride solution in the same way. The mice in all groups except NC group were irradiated with 2.0 Gy⁶⁰Co γ-ray on the fourteenth day. Immune indives of non-specific immune function, cellular immunity and humoral immunity were assessed at the 24th hour after radiation.


RESULTS
Compared with the IC group, the spleen index, thymus index, rate of carbon clearance, phagocytic function of macrophages, lymphocyte proliferation, hemolysin value of blood serum and NK activity were increased markedly (P < 0.05 or P < 0.05).


CONCLUSIONS
RTP1 has an obvious protective effects on damage in γ-ray radiated mice.
Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an, China. liulinna3@126.com
Lin-Na
LN
Liu
Zhi-Wei
ZW
Guo
Yan
Y
Zhang
Hua
H
Qin
Yan
Y
Han
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D005260:Female; D005720:Gamma Rays; D006460:Hemolysin Proteins / Q000097:blood / Q000187:drug effects / Q000528:radiation effects; D007111:Immunity, Cellular / Q000187:drug effects* / Q000528:radiation effects; D056724:Immunity, Humoral / Q000187:drug effects* / Q000528:radiation effects; D007694:Killer Cells, Natural / Q000187:drug effects / Q000528:radiation effects; D008264:Macrophages / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects / Q000528:radiation effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use*; D011134:Polysaccharides / Q000008:administration & dosage / Q000627:therapeutic use*; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000627:therapeutic use; D011897:Random Allocation; D012250:Rheum; D013154:Spleen / Q000187:drug effects / Q000528:radiation effects; D013950:Thymus Gland / Q000187:drug effects / Q000528:radiation effects
D004365:Drugs, Chinese Herbal; D006460:Hemolysin Proteins; D010936:Plant Extracts; D011134:Polysaccharides; D011837:Radiation-Protective Agents