IFN-γ inhibits osteopontin expression in human decidual stromal cells and can be attenuated by 1α,25-dihydroxyvitamin D3.
OBJECTIVE
The effect of IFN-γ on the expression of osteopontin (OPN), in the presence or absence of 1α,25-dihydroxyvitamin D3 (1,25(OH)(2) D(3) ), in decidual stromal cells (DSC).


METHODS
Decidual stromal cells were isolated from women undergoing elective termination of pregnancy (gestational age, 6-9 weeks). After characterization, they were treated with IFN-γ in the presence or absence of 1,25(OH)(2) D(3) . The uterus of pregnancy IFN-γ knockout mice were collected on gestation day (gd) 7.5, and the expression of OPN were examined.


RESULTS
IFN-γ drastically decreased the expression of OPN in DSC, which was reverted by the addition of 1,25(OH)(2) D(3)  to the IFN-γ-treated decidual cells. Moreover, the OPN expression in uterus of IFN-γ knockout mice was higher than that of wild-type counterparts.


CONCLUSIONS
We demonstrated OPN was expressed in DSC in human first-trimester decidua and in the uterus in mice at 7.5 gd. The OPN expression was closely correlated with regulation of IFN-γ and 1,25(OH)(2) D(3)  in human early pregnancy. OPN expression in DSC was significantly decreased with the treatment of IFN-γ. 1,25(OH)(2) D(3)  played an opposite role in IFN-γ-mediated inhibition of OPN expression in human DSC.
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China.
Bin
B
Hu
Hao
H
Zhou
Hui
H
Gao
Yan
Y
Liu
Ting
T
Yan
Li
L
Zou
Lijuan
L
Chen
D000818:Animals; D002117:Calcitriol / Q000276:immunology*; D002478:Cells, Cultured; D003656:Decidua / Q000187:drug effects / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053495:Osteopontin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D017154:Stromal Cells / Q000187:drug effects / Q000276:immunology*; D014599:Uterus / Q000276:immunology*
D053495:Osteopontin; D007371:Interferon-gamma; D002117:Calcitriol