Muscle tissue as an endocrine organ: comparative secretome profiling of slow-oxidative and fast-glycolytic rat muscle explants and its variation with exercise.
The notion that skeletal muscle is a secretory organ capable to release proteins that can act locally in an autocrine/paracrine manner or even in an endocrine manner to communicate with distant tissues has now been recognized. Under this context, a new paradigm has arisen implicating the muscle in metabolism regulation. Considering the evidences that give exercise a protective role against illnesses associated to physical inactivity, it becomes of especial relevance to characterize muscle secreted proteins. In the present study we show for the first time the secretome characterization and the comparative 2-DE secretome analysis among fast-glycolytic (gastrocnemius) and slow-oxidative (soleus) rat muscle explants and its variation after exercise intervention. We have identified 19 differently secreted proteins when comparing soleus and gastrocnemius secretomes, and 10 in gastrocnemius and 17 in soleus distinctive secreted proteins after 1 week of endurance exercise training. Among identified proteins, DJ-1 was found to be more abundant in fast-glycolytic fiber secretomes. On the contrary, FABP-3 was elevated in slow-oxidative fiber secretomes, although its secretion from gastrocnemius muscle increased in exercised animals. These and other secreted proteins identified in this work may be considered as potential myokines.
Grupo Obesidómica, Laboratorio de Endocrinología Molecular y Celular, Instituto de Investigación Sanitaria de Santiago de Compostela, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain.
Arturo
A
Roca-Rivada
Omar
O
Al-Massadi
Cecilia
C
Castelao
Lucía L
LL
Senín
Jana
J
Alonso
Luisa María
LM
Seoane
Tomás
T
García-Caballero
Felipe F
FF
Casanueva
María
M
Pardo
D000818:Animals; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D004702:Endocrine Glands / Q000378:metabolism / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D006019:Glycolysis / Q000502:physiology*; D008297:Male; D055442:Metabolome / Q000235:genetics / Q000502:physiology; D008954:Models, Biological; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism; D009132:Muscles / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D009924:Organ Culture Techniques; D010084:Oxidation-Reduction; D010805:Physical Conditioning, Animal / Q000502:physiology*; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D009124:Muscle Proteins; D020543:Proteome