A highly sensitive fluorescent probe for HClO and its application in live cell imaging.
A new rhodamine-based probe 1 was designed and synthesized as a new fluorescent molecular probe for HClO in PBS buffer at physiological condition. The free probe 1 almost nonfluorescence, however, a drastic enhancement of fluorescence intensity was observed in the presence of HClO. The new probe 1 exhibits good sensitivity and selectivity for HClO over other reactive oxygen and/or nitrogen species in PBS buffer, and the probe was successfully applied to image endogeneous HClO in the living cells.
Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, People's Republic of China.
Qing-Ping
QP
Zuo
Zuo-Jun
ZJ
Li
Yong-Hua
YH
Hu
Bin
B
Li
Li-Hua
LH
Huang
Chun-Jiang
CJ
Wang
Shi-Kun
SK
Liu
Hai-Qiang
HQ
Liao
D000042:Absorption; D000818:Animals; D002021:Buffers; D002460:Cell Line; D002463:Cell Membrane Permeability; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006997:Hypochlorous Acid / Q000378:metabolism*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014867:Water / Q000737:chemistry
D002021:Buffers; D005456:Fluorescent Dyes; D012235:Rhodamines; D014867:Water; D006997:Hypochlorous Acid