Up-regulation of thy-1 promotes invasion and metastasis of hepatocarcinomas.
Increasing evidence has revealed that thy-1 was a potential stem cell marker of liver cancer, but no data have been shown on how thy-1 regulates the pathophysiology of liver cancer, such as proliferation, apoptosis, invasion and migration. We previously demonstrated that thy-1 was expressed in about 1% of hepg2 cells, thy-1+ hepg2 cells, but not thy-1-, demonstrating high tumorigenesis on inoculation 0.5x10⁵ cells per BACA/LA mouse after 2 months. In the present study, our results showed that higher expression of thy-1 occurs in 72% (36/50 cases) of neoplastic hepatic tissues as compared to 40% (20/50 cases) of control tissues, and the expression of thy-1 is higher in poorly differentiated liver tumors than in the well-differentiated ones. In addition, thy-1 expression was detected in 85% of blood samples from liver cancer patients, but none in normal subjects or patients with cirrhosis or hepatitis. There was a significant negative correlation between thy-1 expression and E-cadherin expression (a marker of invasion and migraton), but not between thy-1 expression and AFP expression in all the liver cancer and blood samples. We further investigated the relationship between thy-1 and E- cadherin in liver cancer hepg2 cell line which was transfected with pReceiver-M29/thy-1 eukaryotic expression vector followed by aspirin treatment. Lower expression of E- cadherin but higher expressions of thy-1 were detected in hepg2 cells transfected with pReceiver-M29/thy-1. Taken together, our study suggested that thy-1 probably regulates liver cancer invasion and migration.
Department of Hepatology Center, Fuzhou General Hospital, Nanjing Military Area Command, Fuzhou, China.
Bian-Qiao
BQ
Cheng
Yi
Y
Jiang
Dong-Liang
DL
Li
Jing-Jing
JJ
Fan
Ming
M
Ma
D000704:Analysis of Variance; D001241:Aspirin / Q000494:pharmacology; D015820:Cadherins / Q000187:drug effects / Q000378:metabolism; D002277:Carcinoma / Q000097:blood / Q000378:metabolism* / Q000473:pathology*; D002454:Cell Differentiation; D016009:Chi-Square Distribution; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000097:blood / Q000378:metabolism* / Q000473:pathology*; D018800:Thy-1 Antigens / Q000097:blood / Q000187:drug effects / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation; D000509:alpha-Fetoproteins / Q000187:drug effects / Q000378:metabolism
D015820:Cadherins; D018800:Thy-1 Antigens; D000509:alpha-Fetoproteins; D001241:Aspirin