Microbial transformation of ginsenoside Rg3 to ginsenoside Rh2 by Esteya vermicola CNU 120806.
In the present investigation, we successfully employed a cell-free extract of Esteya vermicola CNU 120806 to convert ginsenoside Rg3 to Rh2. Three important factors including pH, temperature and substrate concentration were optimized for the preparation of Rh(2). The optimal condition was obtained as follows: 50°C, pH 5.0 and substrate concentration of 3 mg ml(-1). The yield of conversion was up to 90.7%. In order to identify the specificity of the β-glucosidase activity of Esteya vermicola CNU 120806, ginsenoside Re (protopanaxatriol saponins) was treated under the same reaction system. Interestingly, no new metabolite was generated, which elucidated that the enzymatic process only occurred by hydrolysis of the terminal glucopyranosyl moieties at the C-3 carbon of ginsenoside Rg(3). The crude enzyme extract can be used for commercial ginsenoside Rh(2) preparation.
Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Taejon, 305-764, Republic of Korea.
Jingang
J
Hou
Jianjie
J
Xue
Chunyan
C
Wang
Lei
L
Liu
Dongliang
D
Zhang
Zhen
Z
Wang
Wei
W
Li
Yinan
Y
Zheng
Changkeun
C
Sung
D001711:Biotransformation; D036145:Ginsenosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D055155:Ophiostomatales / Q000201:enzymology / Q000378:metabolism*; D013696:Temperature; D001617:beta-Glucosidase / Q000378:metabolism
D036145:Ginsenosides; C097367:ginsenoside Rg3; C055305:ginsenoside Rh2; D001617:beta-Glucosidase