Gold nanoparticles for immunogold localization of antigens in plant tissues.
Here we describe postembedding immunoelectron microscopic technique applied to ultrathin sections of plant material. The method relies on the use of gold nanoparticles. The methods include tissue fixation, dehydration, embedding of plant specimens, and staining of semithin and ultrathin sections. The described method is suitable for localization of antigens including proteins (peroxisomal enzymes) and a low molecular substance such as ginsenoide-Rb1.
Division of Functional Morphology, Nagasaki International University, Sasebo, Nagasaki, Japan. syokota@niu.ac.jp
Sadaki
S
Yokota
D052179:Antigens, Plant / Q000032:analysis* / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D006046:Gold / Q000737:chemistry*; D017979:Gold Colloid / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods*; D053768:Metal Nanoparticles; D016253:Microscopy, Immunoelectron / Q000379:methods*; D008867:Microtomy / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
D052179:Antigens, Plant; D001426:Bacterial Proteins; D017979:Gold Colloid; C070001:protein AG-gold complex; D006046:Gold