Promotion effect by ε-poly-L-lysine on the enzymatic reaction of glucose oxidase with ferricyanide ion as an oxidant.
An enzymatic reaction rate of glucose oxidase (GOD) using ferricyanide ion ([Fe(CN)(6)](3-)) as an oxidant significantly increased by the addition of ε-poly-L-lysine (ε-PL). The bimolecular rate constant between GOD and [Fe(CN)(6)](3-) in the presence of ε-PL reached about 10000-fold relative to what it was measured without the ε-PL, and the Michaelis constant decreased. The reaction rate reached a maximum at around pH 6, where ε-PL and GOD possess highly positive and negative charges, respectively. The increment of the reaction rate by ε-PL can be attributed to the electrostatic association of the polycationic ε-PL with the negatively charged GOD to form a polyion complex soluble in the aqueous medium. The adduction of the cationic polymer may relieve the electrostatic repulsion between GOD and [Fe(CN)(6)](3-), so that the electron transfer effectively occurs between them.
Faculty of Biotechnology, Fukui Prefectural University, Eiheiji, Fukui, Japan. kuematsu@fpu.ac.jp
Kohei
K
Uematsu
Misato
M
Yamasaki
Takao
T
Hibi
Hajime
H
Katano
D001234:Aspergillus niger / Q000201:enzymology; D002384:Catalysis; D004563:Electrochemistry; D005292:Ferricyanides / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D007700:Kinetics; D016877:Oxidants / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012996:Solutions; D013053:Spectrophotometry; D055672:Static Electricity
D005292:Ferricyanides; D016877:Oxidants; D011073:Polyamines; D000644:Quaternary Ammonium Compounds; D012996:Solutions; C007931:hexacyanoferrate III; D011107:Polylysine; D005949:Glucose Oxidase