Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli in Japan: emergence of CTX-M-15-producing E. coli ST131.
We investigated the molecular characteristics of ESBL-producing Escherichia coli in Japan. A total of 101 clinical isolates of ESBL-positive E. coli collected in Japan between June 2008 and November 2009 were studied. Among the 101 strains, 97 were positive for CTX-M, while 47 and two were positive for TEM and SHV, respectively. Sequencing revealed that CTX-M-14 was most common (49/101), followed by CTX-M-27 (22/101) and CTX-M-15 (8/101). Based on MLST data, seven of eight CTX-M-15 producers belonged to ST131. This is the first report about clinical isolates of E. coli ST131 possessing CTX-M-15 in Japan.
Department of Infection Control and Laboratory Diagnostics, Tohoku University Graduate School of Medicine, Sendai, 980-8574, Japan.
Hitoshi
H
Kuroda
Hisakazu
H
Yano
Yoichi
Y
Hirakata
Kazuaki
K
Arai
Shiro
S
Endo
Hajime
H
Kanamori
Hiromi
H
Yamamoto
Sadahiro
S
Ichimura
Miho
M
Ogawa
Masahiro
M
Shimojima
Mayumi
M
Komatsu
Teruko
T
Jonai
Sachiko
S
Itagaki
Yuriko
Y
Nonomiya
Akira
A
Suwabe
Mitsuo
M
Kaku
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D017422:Sequence Analysis, DNA; D055815:Young Adult; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D004269:DNA, Bacterial; D001618:beta-Lactamases