Thermodynamic interactions of a cis and trans benzanilide with Escherichia coli bacterial membranes.
The activity of a membrane interactive cis and trans benzanilide against Escherichia coli membrane mimics was investigated using Langmuir monolayers. It was found that in the presence of E. coli lipid mix monolayers, cis-benzanilide induced maximal surface pressure changes of 1 mN m(-1), whereas a reduced interaction was observed with trans-benzanilide. Compression isotherm analysis of these monolayers showed ∆G(mix) < 0, indicating membrane stabilisation by both compounds. The binding coefficient for trans-benzanilide was circa 890 μM and for the cis-benzanilide was 820 μM, suggesting that both benzanilide compounds bound to the E. coli membranes.
School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE, UK.
Sarah R
SR
Dennison
Timothy J
TJ
Snape
David A
DA
Phoenix
D000813:Anilides / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D007536:Isomerism; D013816:Thermodynamics; D053835:Unilamellar Liposomes
D000813:Anilides; D000900:Anti-Bacterial Agents; D053835:Unilamellar Liposomes; C034595:benzanilide