Protocol for gene expression profiling using DNA microarrays in Neisseria gonorrhoeae.
Gene expression profiling using DNA microarrays has become commonplace in current molecular biology practices, and has dramatically enhanced our understanding of the biology of Neisseria spp., and the interaction of these organisms with the host. With the choice of microarray platforms offered for gene expression profiling and commercially available arrays, investigators must ask several central questions to make decisions based on their research focus. Are arrays on hand for their organism and if not then would it be cost-effective to design custom arrays. Other important considerations; what types of specialized equipment for array hybridization and signal detection are required and is the specificity and sensitivity of the array adequate for your application. Here, we describe the use of a custom 12K CombiMatrix ElectraSense™ oligonucleotide microarray format for assessing global gene expression profiles in Neisseria spp.
Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. Lydgia-jackson@ouhsc.edu
Lydgia A
LA
Jackson
David W
DW
Dyer
D018076:DNA, Complementary / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000254:growth & development; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D019800:Phenol / Q000737:chemistry; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D048348:Reverse Transcription; D013194:Staining and Labeling
D018076:DNA, Complementary; D012329:RNA, Bacterial; D019800:Phenol