Colonic hydrogen sulfide-induced visceral pain and referred hyperalgesia involve activation of both Ca(v)3.2 and TRPA1 channels in mice.
Luminal hydrogen sulfide (H(2)S), a gasotransmitter, causes colonic pain / referred hyperalgesia in mice, most probably via activation of T-type Ca(2+) channels. Here we analyzed the mechanisms for H(2)S-induced facilitation of colonic pain signals. Intracolonic administration of NaHS, an H(2)S donor, evoked visceral pain-like nociceptive behavior and referred hyperalgesia in mice, an effect abolished by NNC 55-0396, a selective T-type Ca(2+)-channel blocker, or by knockdown of Ca(v)3.2. AP18, a TRPA1 blocker, also prevented the NaHS-induced colonic pain and referred hyperalgesia. These findings demonstrate that H(2)S-induced colonic pain and referred hyperalgesia require activation of both Ca(v)3.2 and TRPA1 channels in mice.
Division of Pharmacology and Pathophysiology, Kinki University School of Pharmacy, 3-4-1 Kowakae, Higashi-Osaka, Osaka 577-8502, Japan.
Maho
M
Tsubota-Matsunami
Yumi
Y
Noguchi
Yasumasa
Y
Okawa
Fumiko
F
Sekiguchi
Atsufumi
A
Kawabata
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D020747:Calcium Channels, T-Type / Q000378:metabolism*; D003521:Cyclopropanes / Q000494:pharmacology; D005260:Female; D006862:Hydrogen Sulfide / Q000633:toxicity*; D006930:Hyperalgesia / Q000139:chemically induced* / Q000378:metabolism*; D051379:Mice; D009281:Naphthalenes / Q000494:pharmacology; D009619:Nociceptors / Q000378:metabolism; D013440:Sulfides / Q000494:pharmacology; D000074025:TRPA1 Cation Channel; D050051:Transient Receptor Potential Channels / Q000378:metabolism*; D059265:Visceral Pain / Q000139:chemically induced / Q000378:metabolism*
D001562:Benzimidazoles; C504883:Cacna1h protein, mouse; D020747:Calcium Channels, T-Type; D003521:Cyclopropanes; D009281:Naphthalenes; D013440:Sulfides; D000074025:TRPA1 Cation Channel; D050051:Transient Receptor Potential Channels; C475685:Trpa1 protein, mouse; C484287:NNC 55-0396; C025451:sodium bisulfide; D006862:Hydrogen Sulfide