Evaluation of Clearview Exact PBP2a, a new immunochromatographic assay, for detection of low-level methicillin-resistant Staphylococcus aureus (LL-MRSA).
We evaluated the performance of a new immunochromatographic assay (ICA), the Clearview Exact PBP2a, for rapid detection of penicillin-binding protein 2a (PBP2a) in a challenge set of Staphylococcus aureus strains showing MICs to oxacillin of ≤16 mg/liter. The sensitivity and specificity of the ICA were 96.6% and 100%, respectively.
Laboratoire de Référence MRSA-Staphylocoques, Service de Microbiologie, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium. claire.nonhoff@erasme.ulb.ac.be
Claire
C
Nonhoff
Sandrine
S
Roisin
Marie
M
Hallin
Olivier
O
Denis
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D010068:Oxacillin / Q000494:pharmacology; D046915:Penicillin-Binding Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D046915:Penicillin-Binding Proteins; D010068:Oxacillin