Restriction fragment length analysis of the cytochrome b gene and muscle fatty acid composition differentiate the cryptic flatfish species Solea solea and Solea aegyptiaca.
Overlapping external morphometric characters easily confound the flatfishes  Solea aegyptiaca  and  Solea solea  (Soleidae) in areas of the Mediterranean Sea where both species live in sympatry. This leads to uncertainties in the fisheries and marketing of the species, in addition to misinterpretations in biogeography and conservation studies. This paper describes a simple restriction fragment length-based diagnostic test that differentiates S. solea from S. aegyptiaca, as well as from other species of the Soleidae family. Furthermore, the two species living in sympatry in the Gulf of Kavala (North Aegean Sea, Greece) present significant qualitative differences in muscle fatty acid composition, a property that can also be used to distinguish the two cryptic species.
National Agricultural Research Foundation, Fisheries Research Institute, Kavala, Greece.
Evridiki
E
Boukouvala
Alessia
A
Cariani
Gregory E
GE
Maes
Rafael G
RG
Sevilla
Véronique
V
Verrez-Bagnis
Marc
M
Jérôme
Ilaria
I
Guarniero
Georgios
G
Monios
Fausto
F
Tinti
Filip A M
FA
Volckaert
José M
JM
Bautista
Grigorios
G
Krey
D000818:Animals; D045303:Cytochromes b / Q000235:genetics*; D005227:Fatty Acids / Q000032:analysis*; D005260:Female; D005412:Flatfishes / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D063427:Food Quality; D008297:Male; D008522:Mediterranean Sea; D009132:Muscles / Q000737:chemistry*; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D005227:Fatty Acids; D045303:Cytochromes b