Characterization of phenolic compounds and antinociceptive activity of Sempervivum tectorum L. leaf juice.
Sempervivum tectorum L. (houseleek) leaf juice has been known as a traditional herbal remedy. The aim of the present study was the chemical characterization of its phenolic compounds and to develop quantitation methods for its main flavonol glycoside, as well as to evaluate its antinociceptive activity. Lyophilized houseleek leaf juice was studied by HPLC-DAD coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) to identify flavonol glycosides, hydroxy-benzoic and hydroxy-cinnamic acids. Ten flavonol glycosides and sixteen phenolic acid compounds were identified or tentatively characterized. Structure of the main flavonol compound was identified by nuclear magnetic resonance spectroscopy. Three characteristic kaempferol glycosides were isolated and determined by LC-ESI-MS/MS with external calibration method, using the isolated compounds as standard. The main flavonol glycoside was also determined by HPLC-DAD. Validated HPLC-DAD and LC-ESI-MS/MS methods were developed to quantify kaempferol-3-O-rhamnosyl-glucoside-7-O-rhamnoside and two other kaempferol glycosides. Antinociceptive activity of houseleek leaf juice was investigated by writhing test of mice. Sempervivum extract significantly reduced pain in the mouse writhing test.
Semmelweis University, Department of Pharmacognosy, Ülloi u. 26., H1085 Budapest, Hungary. albertiagnes@gmail.com
Ágnes
Á
Alberti
Szabolcs
S
Béni
Erzsébet
E
Lackó
Pál
P
Riba
Mahmoud
M
Al-Khrasani
Ágnes
Á
Kéry
D019342:Acetic Acid; D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D029627:Crassulaceae / Q000737:chemistry; D004195:Disease Models, Animal; D005612:Freeze Drying; D006027:Glycosides / Q000494:pharmacology; D044949:Kaempferols / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy / Q000592:standards; D008297:Male; D051379:Mice; D015394:Molecular Structure; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000592:standards
D000700:Analgesics; D006027:Glycosides; D044949:Kaempferols; D010636:Phenols; D010936:Plant Extracts; D019342:Acetic Acid