An insight into the mechanisms of nanoceria toxicity in aquatic photosynthetic organisms.
The effect of nanoceria on two aquatic photosynthetic organisms of ecological relevance, a green alga and a cyanobacterium, is reported. The main bioenergetic process of these organisms, photosynthesis, was studied by measuring both oxygen evolution and chlorophyll a fluorescence emission parameters. Nanoceria significantly inhibited photosynthesis in the cyanobacterium in the entire range of concentrations tested (0.01-100 mg/L), while a dual effect of nanoceria was found in the green alga with slight stimulation at low concentrations and strong inhibition at the highest concentrations tested. Chlorophyll a fluorescence experiments indicated that nanoceria had a significant impact on the primary photochemical processes of photosystem II. The primary cause of the observed photosynthetic inhibition by nanoceria is an excessive level of ROS formation; the results indicated a strong generation of reactive oxygen species (ROS) which caused oxidative damage, as evidenced by lipid peroxidation in both photosynthetic organisms. It is proposed that nanoceria can increase the production of hydrogen peroxide (a normal ROS by-product of light-driven photosynthesis) in both the green alga and the cyanobacterium; through an oxidative reaction, these ROS cause lipid peroxidation, compromising membrane integrity and also seriously impairing photosynthetic performance, eventually leading to cell death.
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, E-28049, Madrid, Spain.
Ismael
I
Rodea-Palomares
Soledad
S
Gonzalo
Javier
J
Santiago-Morales
Francisco
F
Leganés
Eloy
E
García-Calvo
Roberto
R
Rosal
Francisca
F
Fernández-Piñas
D017033:Anabaena / Q000187:drug effects*; D002563:Cerium / Q000633:toxicity*; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D000460:Chlorophyta / Q000187:drug effects*; D010788:Photosynthesis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D002563:Cerium; C030583:ceric oxide; D000077194:Chlorophyll A