Ethanol production from sweet sorghum juice in repeated-batch fermentation by Saccharomyces cerevisiae immobilized on corncob.
Ethanol fermentation from sweet sorghum juice containing 240 g/l of total sugar by Saccharomyces cerevisiae TISTR 5048 and S. cerevisiae NP 01 immobilized on low-cost support materials, corncob pieces, was investigated. In batch fermentation, S. cerevisiae TISTR 5048 immobilized on 6 × 6 × 6 mm(3) corncobs gave higher ethanol production than those immobilized on 12 × 12 × 12 mm(3) corncobs in terms of ethanol concentration (P), yield (Y                         (                            p/s                         )) and productivity (Q                         (                            p                         )) with the values of 102.39 ± 1.11 g/l, 0.48 ± 0.01 and 2.13 ± 0.02 g/l h, respectively. In repeated-batch fermentation, the yeasts immobilized on the 6 × 6 × 6 mm(3) corncobs could be used at least eight successive cycles with the average P, Y                         (                            p/s                         ) and Q                         (                            p                         ) of 97.19 ± 5.02 g/l, 0.48 ± 0.02 and 2.02 ± 0.11 g/l h, respectively. Under the same immobilization and repeated-batch fermentation conditions, P (90.75 ± 3.05 g/l) and Q                         (                            p                         ) (1.89 ± 0.06 g/l h) obtained from S. cerevisiae NP 01 were significantly lower than those from S. cerevisiae TISTR 5048 (P < 0.05), while Y                         (                            p/s                         ) from both strains were not different. S. cerevisiae TISTR 5048 immobilized on the corncobs also gave significantly higher P, Y                         (                            p/s                         ) and Q                         (                            p                         ) than those immobilized on calcium alginate beads (P < 0.05).
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand.
Lakkana
L
Laopaiboon
Pattana
P
Laopaiboon
D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D045868:Sorghum / Q000737:chemistry*
D000431:Ethanol