Topical application of Sadat-Habdan mesenchymal stimulating peptide (SHMSP) accelerates wound healing in diabetic rabbits.
OBJECTIVE
Diminished wound healing is a common problem in diabetic patients due to diminished angiogenesis. SHMSP was found to promote angiogenesis. The present study was carried out to examine the effect of this peptide in healing of wounds in diabetic rabbits.


METHODS
Twenty male New Zealand rabbits were used in this study. Diabetes mellitus was induced and the rabbits were randomly divided into two equal groups: control group and peptide group. A-full thickness punch biopsy was made to create a wound of about 10 mm on the right ears of all rabbits. Every day, the wound was cleaned with saline in control groups. In the peptide group, 15 mg of SHMSP was applied after cleaning. On day 15th, all animals were sacrificed, and the wounds were excised with a rim of 5 mm of normal surrounding tissue. Histo-pathological assessment of wound healing, inflammatory cell infiltration, blood vessel proliferation, and collagen deposition was performed.


RESULTS
There were no deaths among the groups. There was significant increase in wound healing, blood vessel proliferation and collagen deposition, and significant decrease in inflammatory cell infiltration in the peptide group compared to the control group.


CONCLUSIONS
Topical application of SHMSP improves wound healing in diabetic rabbits.
Department of Internal Medicine, University of Dammam, Dammam, Saudi Arabia.
Abdulmohsen H
AH
Al-Elq
Mir
M
Sadat-Ali
Mohamed
M
Elsharawy
Ibrahim
I
Al-Habdan
Fatin Othman
FO
Al-Aqeel
Magda M
MM
Naim
D000287:Administration, Topical; D000818:Animals; D001706:Biopsy; D001808:Blood Vessels / Q000473:pathology; D003094:Collagen / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D006943:Hyperglycemia / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism; D008297:Male; D009389:Neovascularization, Pathologic; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D014945:Wound Healing
D010455:Peptides; C530179:Sadat-Habdan mesenchymal stimulating peptide; D003094:Collagen