H3K27 methylation and H3S28 phosphorylation-dependent transcriptional regulation by INHAT subunit SET/TAF-Iβ.
Significant progress has been made in understanding the relationship between histone modifications and 'reader' molecules and their effects on transcriptional regulation. A previously identified INHAT complex subunit, SET/TAF-Iβ, binds to histones and inhibits histone acetylation. To investigate the binding specificities of SET/TAF-Iβ to various histone modifications, we employed modified histone tail peptide array analyses. SET/TAF-Iβ strongly recognized PRC2-mediated H3K27me1/2/3; however, the bindings were completely disrupted by H3S28 phosphorylation. We have demonstrated that SET/TAF-Iβ is sequentially recruited to the target gene promoter ATF3 after the PRC2 complex via H3K27me recognition and may offer additive effects in the repression of the target gene.
Department of Life Science, College of Natural Sciences, Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
Ji-Young
JY
Kim
Kee-Beom
KB
Kim
Hye-Ju
HJ
Son
Yun-Cheol
YC
Chae
Si-Taek
ST
Oh
Dong-Wook
DW
Kim
Jhang Ho
JH
Pak
Sang-Beom
SB
Seo
D051700:Activating Transcription Factor 3 / Q000235:genetics; D004268:DNA-Binding Proteins; D063185:Epigenetic Repression; D006367:HeLa Cells; D056488:Histone Chaperones / Q000378:metabolism*; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D008962:Models, Theoretical; D010766:Phosphorylation; D063151:Polycomb Repressive Complex 2 / Q000378:metabolism; D011401:Promoter Regions, Genetic; D040081:Protein Array Analysis; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D012097:Repressor Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
C490449:ATF3 protein, human; D051700:Activating Transcription Factor 3; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D006657:Histones; D012097:Repressor Proteins; C513473:SET protein, human; D014157:Transcription Factors; D063151:Polycomb Repressive Complex 2