Impact of transgenic cotton varieties on activity of enzymes in their rhizosphere.
The impact of five Bacillus thuringiensis (Bt) cotton varieties and their respective isogenic non-Bt(NBt) isolines (ANKUR-2534, MECH-6304, RCH-317, ANKUR-651 and MECH-6301) was assessed on the key soil enzymes i.e., dehydrogenase, alkaline phosphatase and urease in their rhizosphere at four growth stages of the crop, namely vegetative, flowering, bolling and harvesting. These varieties were grown on farmer's field in villages 22 miles and 24 miles of Ganganagar District of Rajasthan State in India. Results showed that dehydrogenase, alkaline phosphatase and urease activities were higher in rhizosphere of Bt isolines as compared to NBt isolines of all the varieties. Except phosphatase, differences in dehydrogenase and urease activities in rhizosphere of Bt and NBt isolines of all five varieties were significant (P < 0.05). Maximum enhancement in the three enzymes activities was observed in MECH-6304 Bt isoline rhizosphere. Maximum and minimum activities of dehydrogenase and urease were observed in MECH-6304 and RCH-317 Bt isolines, respectively, whereas phosphatase activity was maximum and minimum in MECH-6304 and ANKUR-651 Bt isolines, respectively. Maximum dehydrogenase and urease activities were observed at boll formation and minimum at flowering and harvesting stage, respectively, while maximum phosphatase activity was observed at vegetative stage and minimum at harvesting stage. In conclusion, all the studied Bt isolines of cotton varieties showed no adverse effect on dehydrogenase, alkaline phosphatase and urease activities in the rhizosphere.
Division of Environmental Sciences, Indian Agricultural Research Institute, New Delhi 110012, India. usha_env@iari.res.in
Usha
U
Mina
Anita
A
Chaudhary
D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism; D001413:Bacillus thuringiensis / Q000201:enzymology / Q000235:genetics*; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D030821:Plants, Genetically Modified; D058441:Rhizosphere; D012987:Soil / Q000032:analysis; D014510:Urease / Q000737:chemistry / Q000378:metabolism
D012987:Soil; D010088:Oxidoreductases; D000469:Alkaline Phosphatase; D014510:Urease