A fast response highly selective probe for the detection of glutathione in human blood plasma.
A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.
Department of Chemistry, Portland State University, Portland, OR 97201, USA. yixing@pdx.edu
Yixing
Y
Guo
Xiaofeng
X
Yang
Lovemore
L
Hakuna
Aabha
A
Barve
Jorge O
JO
Escobedo
Mark
M
Lowry
Robert M
RM
Strongin
D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000097:blood*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
D005456:Fluorescent Dyes; D005978:Glutathione