Templated chemistry for monitoring damage and repair directly in duplex DNA.
We report the fluorogenic detection of the product of base excision repair (an abasic site) in a specific sequence of duplex DNA. This is achieved by DNA-templated chemistry, employing triple helix-forming probes that contain unnatural nucleobases designed to selectively recognize the site of a missing base. Light-up signals of up to 36-fold were documented, and probes could be used to monitor enzymatic removal of a damaged base.
Department of Chemistry, Stanford University, Stanford, California 94305, USA.
Seoung Ho
SH
Lee
Shenliang
S
Wang
Eric T
ET
Kool
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D015342:DNA Probes / Q000737:chemistry*; D004260:DNA Repair; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence
D015342:DNA Probes; D004247:DNA