Trichoderma asperellumChi42 Genes Encode Chitinase.
Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.
Institute of Resources, Environment and Biotechnology, Hue University, 27 Phan Dinh Phung St., Hue, Vietnam.
Nguyen Hoang
NH
Loc
Hoang Tan
HT
Quang
Nguyen Bao
NB
Hung
Nguyen Duc
ND
Huy
Truong Thi Bich
TT
Phuong
Tran Thi Thu
TT
Ha