The osteogenic study of tissue engineering bone with BMP2 and BMP7 gene-modified rat adipose-derived stem cell.
To evaluate the feasibility and advantages of constructing a novel tissue engineering bone, using β-tricalcium phosphate (β-TCP) and rat adipose-derived stem cells (ADSCs), modified with BMP2 and BMP7 by lentivirus. In the present study, ADSCs transfected with Lv-BMP2 and Lv-BMP7, alone or together, were seeded on β-TCP scaffold and cultured in vitro. Based on the results of DNA assay, alkaline phosphatase (ALP) activity, alizarin red staining and osteogenic marker genes expression analysis, the BMP2 and BMP7 genes cotransfection group exhibited a higher degree of osteogenic differentiation in vitro. To investigate the in vivo osteogenesis of the tissue engineering bone, the ADSCs/β-TCP constructs were implanted in rat femurs defects for 6 weeks and studied histomorphology and radiography. The results showed that BMP2 and BMP7 genes cotransfection group dramatically enhanced the efficiency of new bone formation than BMP2 group and BMP7 group in vivo. These results demonstrated that it was advantageous to construct tissue engineering bone using ADSCs cotransfected with BMP2 and BMP7 on β-TCP, providing a potential way for treating bone defects.
Center for Joint Surgery, South West Hospital, Third Military Medical University, Chongqing 400038, China.
Wang
W
Qing
Chen
C
Guang-Xing
Guo
G
Lin
Yang
Y
Liu
D000273:Adipose Tissue / Q000166:cytology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics* / Q000378:metabolism; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics* / Q000378:metabolism; D001861:Bone Regeneration / Q000235:genetics / Q000502:physiology; D002130:Calcium Phosphates; D048708:Cell Growth Processes / Q000502:physiology; D005269:Femur / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014162:Transfection; D014945:Wound Healing / Q000235:genetics / Q000502:physiology
D000880:Anthraquinones; C525718:BMP2 protein, human; C525819:BMP7 protein, human; D055396:Bone Morphogenetic Protein 2; D055419:Bone Morphogenetic Protein 7; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; C004468:Alizarin Red S; D000469:Alkaline Phosphatase