Live-cell super-resolution imaging goes multicolor.
New resolutions: The combined use of photoactivatable fluorescent proteins and synthetic fluorophores considerably expands our options for multicolor super-resolution fluorescence imaging and enables for the first time the simultaneous imaging of more than two proteins with subdiffraction optical resolution in living cells.
Department of Biotechnology & Biophysics, Biozentrum, Julius-Maximilians-Universität Würzburg, Am Hubland, Würzburg, Germany.
Teresa
T
Klein
Sebastian
S
van de Linde
Markus
M
Sauer
D002460:Cell Line; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D008958:Models, Molecular; D011506:Proteins / Q000032:analysis* / Q000378:metabolism
D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011506:Proteins