Efficient access to highly pure β-amyloid peptide by optimized solid-phase synthesis.
Feasible and reproducible synthesis of full-length Aβ peptides has been one of the major challenges in Alzheimer's disease research. By using dimethyl sulfoxide as an anti-aggregation solvent and as an agent to promote double-coupling of two phenylalanine that frequently experience residual deletion, we developed a reliable manual Fmoc solid phase peptide synthesis procedure to produce biologically active Aβ in large quantities at relatively high purity. The amyloidogenic activity of the synthesized Aβ was confirmed via thioflavin T assay, transmission electron microscopic analysis and electrophoresis.
Center for Neuro-Medicine, Brain Science Institute, Korea Institute of Science and Technology , Hwarangno 14-gil 5, Seongbuk-gu, Seoul , Republic of Korea.
Ji Won
JW
Choi
Hye Yun
HY
Kim
MiJin
M
Jeon
Dong Jin
DJ
Kim
YoungSoo
Y
Kim
D000596:Amino Acids / Q000737:chemistry; D000682:Amyloid / Q000737:chemistry / Q000648:ultrastructure; D016229:Amyloid beta-Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D052160:Benzothiazoles; D002851:Chromatography, High Pressure Liquid; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D060327:Solid-Phase Synthesis Techniques; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013844:Thiazoles / Q000737:chemistry
D000596:Amino Acids; D000682:Amyloid; D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D005449:Fluorenes; D005456:Fluorescent Dyes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D012997:Solvents; D013844:Thiazoles; C009462:thioflavin T; D004121:Dimethyl Sulfoxide