Sex-specific expression of estrogen receptors α and β and Kiss1 in the postnatal rat amygdala.
The rodent amygdaloid complex is composed of numerous subnuclei important for the sex-specific regulation of sociosexual behavior. Although estrogen receptors (ERs) are critical for organizing functional and cytoarchitectural sex differences in these subnuclei, a detailed developmental profile of ER expression in the amygdaloid complex is not available. Moreover, the kisspeptin gene (Kiss1) was recently identified in the adult amygdala, but it remains unknown if it is expressed during development. To fill these data gaps, rat brains (5-7/group) were assessed on postnatal days (PNDs) 0, 2, 4, 7, and 19 for ER alpha (ERα; Esr1), beta (ERβ; Esr2), and Kiss1 expression using in situ hybridization. Expression was quantified in the posterodorsal portion of the medial amygdala posterodorsal (MePD), lateral (PLCo), and medial (PMCo) components of the posterior cortical nucleus, and the amygdalohippocampal area (AHi). ERα expression was high throughout the amygdala at birth, but sexually dimorphic only in the AHi. ERα expression in the MePD and the PLCo showed a U-shaped expression pattern over time. In the PMCo, ERα expression decreased from PND 2 and remained low through PND 19. Sexually dimorphic expression of ERβ in the MePD was observed on PND 0, with higher levels in females, but reversed by PND 4 due to declining levels in females. No Kiss1 signal was observed in the postnatal amygdala, suggesting that expression arises after puberty. These data reveal that ER expression is region-specific within the neonatal amygdala. These differences likely contribute to sex differences in sociosexual behavior across the lifespan.
Department of Biology, North Carolina State University, Raleigh, North Carolina 27695, USA.
Jinyan
J
Cao
Heather B
HB
Patisaul
D000679:Amygdala / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D047628:Estrogen Receptor alpha / Q000096:biosynthesis* / Q000235:genetics; D047629:Estrogen Receptor beta / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006624:Hippocampus / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D017403:In Situ Hybridization; D059648:Kisspeptins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D020318:Rats, Long-Evans; D012727:Sex Characteristics; D012741:Sexual Maturation
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; C491406:Kiss1 protein, rat; D059648:Kisspeptins; D012333:RNA, Messenger