The role of haemoglobin mass on VO(2)max following normobaric 'live high-train low' in endurance-trained athletes.
It remains unclear by which mechanism 'live high-train low' (LHTL) altitude training increases exercise performance. Haematological and skeletal muscle adaptations have both been proposed. To test the hypotheses that (i) LHTL improves maximal oxygen uptake (VO(2)max) and (ii) this improvement is related to hypoxia-induced increases in total haemoglobin mass (Hb(mass)) and not to improved maximal oxidative capacity of skeletal muscle, we determined VO(2)max before LHTL and after LHTL, before and after the altitude-induced increases in Hb(mass) (measured by carbon-monoxide rebreathing) had been abolished by isovolumic haemodilution. We obtained skeletal muscle biopsies to quantify mitochondrial oxidative capacity and efficiency. Sixteen endurance-trained athletes were assigned (double-blinded, placebo controlled) to ≥16 h/day over 4 weeks to normoxia (placebo, n=6) or normobaric hypoxia equivalent to 3000 m altitude (LHTL, n=10). Four-week LHTL did not increase VO(2)max, irrespective of treatment (LHTL: 1.5%; placebo: 2.0%). Hb(mass) was slightly increased (4.6%) in 5 (of 10) LHTL subjects but this was not accompanied by a concurrent increase in VO(2)max. In the subjects demonstrating an increase in Hb(mass), isovolumic haemodilution elicited a 5.8% decrease in VO(2)max. Cycling efficiency was altered neither with time nor by LHTL. Neither maximal capacity of oxidative phosphorylation nor mitochondrial efficiency was modified by time or LHTL. The present results suggest that LHTL has no positive effect on VO(2)max in endurance-trained athletes because (i) muscle maximal oxidative capacity is not improved following LHTL and (ii) erythrocyte volume expansion after LHTL, if any, is too small to alter O(2) transport.
Département Médical, Ecole Nationale de Ski et d'Alpinisme, site de l'Ecole Nationale des Sports de Montagne, Chamonix, France.
Paul
P
Robach
Christoph
C
Siebenmann
Robert A
RA
Jacobs
Peter
P
Rasmussen
Nikolai
N
Nordsborg
Dominik
D
Pesta
Erich
E
Gnaiger
Víctor
V
Díaz
Andreas
A
Christ
Julia
J
Fiedler
Nadine
N
Crivelli
Niels H
NH
Secher
Aurélien
A
Pichon
Marco
M
Maggiorini
Carsten
C
Lundby
D000328:Adult; D000531:Altitude; D015444:Exercise / Q000502:physiology*; D017079:Exercise Tolerance / Q000502:physiology; D005260:Female; D006438:Hemodilution / Q000379:methods; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008931:Mitochondria, Muscle / Q000502:physiology; D018482:Muscle, Skeletal / Q000502:physiology; D010101:Oxygen Consumption / Q000502:physiology*; D012111:Residence Characteristics
D006454:Hemoglobins