Label free fluorescence turn-on detection of polynucleotide kinase activity with a perylene probe.
A single stranded oligonucleotide could induce aggregation of a perylene probe, the probe's monomer fluorescence was efficiently quenched. However, when the oligonucleotide was 5'-phosphorylated by polynucleotide kinase, it could be very efficiently degraded by lambda exonuclease, probe monomers were released, and a turn on fluorescence signal was detected.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
Huping
H
Jiao
Bin
B
Wang
Jian
J
Chen
Dongli
D
Liao
Wenying
W
Li
Cong
C
Yu
D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D005456:Fluorescent Dyes; D009841:Oligonucleotides; D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins; D010569:Perylene; D011116:Polynucleotide 5'-Hydroxyl-Kinase