Shewanella frigidimarina microbial fuel cells and the influence of divalent cations on current output.
The genes involved in the proposed pathway for Shewanella extracellular electron transfer (EET) are highly conserved. While extensive studies involving EET from a fresh water Shewanella microbe (S. oneidensis MR-1) to soluble and insoluble electron acceptors have been published, only a few reports have examined EET from marine strains of Shewanella. Thus, Shewanella frigidimarina (an isolate from Antarctic Sea ice) was used within miniature microbial fuel cells (mini-MFC) to evaluate potential power output. During the course of this study several distinct differences were observed between S. oneidensis MR-1 and S. frigidimarina under comparable conditions. The maximum power density with S. frigidimarina was observed when the anolyte was half-strength marine broth (1/2 MB) (0.28 μW/cm(2)) compared to Luria-Bertani (LB) (0.07 μW/cm(2)) or a defined growth minimal medium (MM) (0.02 μW/cm(2)). The systematic modification of S. frigidimarina cultured in 1/2 MB and LB with divalent cations shows that a maximum current output can be generated independent of internal ionic ohmic losses and the presence of external mediators.
Chemistry Division, US Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC 20375, USA.
Lisa A
LA
Fitzgerald
Emily R
ER
Petersen
Dagmar H
DH
Leary
Lloyd J
LJ
Nadeau
Carissa M
CM
Soto
Richard I
RI
Ray
Brenda J
BJ
Little
Bradley R
BR
Ringeisen
Glenn R
GR
Johnson
Gary J
GJ
Vora
Justin C
JC
Biffinger
D001674:Bioelectric Energy Sources / Q000382:microbiology*; D002413:Cations, Divalent; D004566:Electrodes; D004579:Electron Transport; D004735:Energy Transfer; D004867:Equipment Design; D019544:Equipment Failure Analysis; D020592:Shewanella / Q000145:classification / Q000502:physiology*; D013045:Species Specificity
D002413:Cations, Divalent