Conductive carbon tape used for support and mounting of both whole animal and fragile heat-treated tissue sections for MALDI MS imaging and quantitation.
Analysis of whole animal tissue sections by MALDI MS imaging (MSI) requires effective sample collection and transfer methods to allow the highest quality of in situ analysis of small or hard to dissect tissues. We report on the use of double-sided adhesive conductive carbon tape during whole adult rat tissue sectioning of carboxymethyl cellulose (CMC) embedded animals, with samples mounted onto large format conductive glass and conductive plastic MALDI targets, enabling MSI analysis to be performed on both TOF and FT-ICR MALDI mass spectrometers. We show that mounting does not unduly affect small molecule MSI detection by analyzing tiotropium abundance and distribution in rat lung tissues, with direct on-tissue quantitation achieved. Significantly, we use the adhesive tape to provide support to embedded delicate heat-stabilized tissues, enabling sectioning and mounting to be performed that maintained tissue integrity on samples that had previously been impossible to adequately prepare section for MSI analysis. The mapping of larger peptidomic molecules was not hindered by tape mounting samples and we demonstrate this by mapping the distribution of PEP-19 in both native and heat-stabilized rat brains. Furthermore, we show that without heat stabilization PEP-19 degradation fragments can detected and identified directly by MALDI MSI analysis.
Global Distribution Imaging, DMPK IM, AstraZeneca R&D, Södertälje, Sweden; Medical Mass Spectrometry, Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden. Electronic address: richard.goodwin@astrazeneca.com.
Richard J A
RJA
Goodwin
Medical Mass Spectrometry, Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden.
Anna
A
Nilsson
Global Distribution Imaging, DMPK IM, AstraZeneca R&D, Södertälje, Sweden.
Daniel
D
Borg
SIRCAMS, School of Chemistry, University of Edinburgh, Edinburgh, UK.
Pat R R
PRR
Langridge-Smith
Medical and Biological Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife, UK.
David J
DJ
Harrison
SIRCAMS, School of Chemistry, University of Edinburgh, Edinburgh, UK.
C Logan
CL
Mackay
Global Distribution Imaging, DMPK IM, AstraZeneca R&D, Södertälje, Sweden.
Suzanne L
SL
Iverson
Medical Mass Spectrometry, Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden.
Per E
PE
Andrén
D000818:Animals; D002244:Carbon / Q000494:pharmacology; D003952:Diagnostic Imaging; D006652:Histological Techniques; D006358:Hot Temperature; D008297:Male; D008867:Microtomy; D016612:Paraffin Embedding; D051381:Rats; D012149:Restraint, Physical / Q000379:methods / Q000502:physiology; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D053832:Surgical Tape / Q000706:statistics & numerical data; D013814:Thermal Conductivity; D051598:Whole Body Imaging / Q000379:methods / Q000662:veterinary
D002244:Carbon