Alteration of renal functional, oxidative stress and inflammatory indices following hepatic ischemia-reperfusion.
Liver ischemia/reperfusion (IR) injury is a complex phenomenon that may cause local as well as remote organ injuries. Reactive oxygen species (ROS) along with many pro- and anti- inflammatory cytokines are implicated in the development of organ injury. The renal functional, histological, oxidative stress and inflammatory indices were studied during a short and a longer period of liver IR. Rats were subjected to either sham operation or 90 min partial liver ischemia followed by 4 or 24 h of reperfusion. Serum ALT, AST, ALK and LDH levels, BUN and creatinine, renal MDA level, SOD and catalase activities were evaluated as well as serum IL-6 and IL-10 concentrations along with renal histological evaluation. Ninety minutes liver ischemia /4 h reperfusion caused an increase in BUN and renal MDA levels and a decrease in SOD and catalase activities. It also caused an increase in serum IL-6 and IL-10 levels. 24 h liver reperfusion resulted in a reduction in BUN levels and lower oxidative damages demonstrated by a decrease in renal MDA levels and an increase in renal SOD and catalase activities comparing to 4 h reperfusion group. Evaluations indicated improvement in histology such as less cytoplasmic vacuolation and lower tubular debris. Serum inflammatory indices (IL-6 and IL-10 levels) were also reduced. This study showed that liver IR damage causes renal injury including functional, inflammatory and oxidative status changes. The remote kidney damage was then improved by continuing reperfusion from 4 to 24 h.
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. kadkhodm@tums.ac.ir
Mehri
M
Kadkhodaee
Saideh
S
Mikaeili
Maryam
M
Zahmatkesh
Freshteh
F
Golab
Behjat
B
Seifi
Hossein-Ali
HA
Arab
Sedigheh
S
Shams
Mitra
M
Mahdavi-Mazdeh
D000818:Animals; D007668:Kidney / Q000503:physiopathology*; D007677:Kidney Function Tests; D008099:Liver / Q000098:blood supply* / Q000503:physiopathology*; D008297:Male; D009393:Nephritis / Q000209:etiology* / Q000503:physiopathology*; D018384:Oxidative Stress; D051381:Rats; D015427:Reperfusion Injury / Q000150:complications* / Q000503:physiopathology*