[Plant regeneration of Withania somnifera].
OBJECTIVE
To study tissue culture and plant regeneration of Withania somnifera.


METHODS
Leaves of W. somnifera were used for explants, effects of different plant growth substances on callus and shoot induction were studied, different medium and plant growth substances for rooting induction was optimized.


CONCLUSIONS
The best plant growth substances combination for callus induction was MS + 1.0 mg x L(-1) 2,4-D + 0.1 mg x L(-1) KT. The optimal medium for germination was MS + 1.0 mg x L(-1) 6-BA + 0.1 mg x L(-1) NAA. The best medium and plant growth substances combination for rooting induction was 1/2MS + 0.5 mg x L(-1) NAA, transplant survival rate of plantlet reached 92% in humus soil-pearlite (1:1).
SchooL of Life Sciense, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China.
Zhengwei
Z
Luo
Yiming
Y
Sun
Cuiping
C
Lv
Fengying
F
Wang
Jiwei
J
Sun
Min
M
Sun
D003470:Culture Media / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects*; D046509:Tissue Culture Techniques; D032341:Withania / Q000187:drug effects / Q000254:growth & development*
D003470:Culture Media; D010937:Plant Growth Regulators