Molecular cloning and functional expression of two key carotene synthetic genes derived from Blakeslea trispora into E. coli for increased β-carotene production.
Blakeslea trispora is used commercially to produce β-carotene. Isopentenyl pyrophosphate isomerase (IPI) and geranylgeranyl pyrophosphate synthase (GGPS) are key enzymes in the biosynthesis of carotenoids. The cDNAs of genes ipi and carG were cloned from the fungus and expressed in Escherichia coli. Greater GGPS activity was needed in the engineered E. coli when IPP activity was increased. The introduction of GGPS and IPI increased the β-carotene content in E. coli from 0.5 to 0.95 mg/g dry wt.
State Key Laboratory of Chemical Resource Engineering, College of Life Science & Technology, Beijing University of Chemical Technology, Beijing, China. sunjiehh@126.com
Jie
J
Sun
Xin-Xiao
XX
Sun
Ping-Wah
PW
Tang
Qi-Peng
QP
Yuan
D019748:Carbon-Carbon Double Bond Isomerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D045782:Hemiterpenes; D053858:Metabolic Networks and Pathways; D009090:Mucorales / Q000201:enzymology* / Q000235:genetics*; D010802:Phylogeny; D019207:beta Carotene / Q000096:biosynthesis*
D005656:Fungal Proteins; D045782:Hemiterpenes; D019207:beta Carotene; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase