MicroRNA-145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB.
MicroRNAs (miRNAs) are a class of 21- to 25-nucleotide non-coding RNAs, some of which are important gene regulators involved in folliculogenesis. In this study, we used CCK-8, real-time PCR and Western blot assays to demonstrate that miR-145 inhibits mouse granulosa cell (mGC) proliferation. Combined with the results of luciferase reporter assays that studied the 3'-untranslated region of ACVRIB mRNA, these assays identified ACVRIB as a direct target of miR-145. The ectopic expression of miR-145 reduced the levels of both ACVRIB mRNA and protein and also interfered with activin-induced Smad2 phosphorylation. Altogether, this study revealed that miR-145 suppresses mGC proliferation by targeting ACVRIB.
Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, People's Republic of China.
Guijun
G
Yan
Lianxiao
L
Zhang
Ting
T
Fang
Qun
Q
Zhang
Shaogen
S
Wu
Yue
Y
Jiang
Haixiang
H
Sun
Yali
Y
Hu
D020413:3' Untranslated Regions; D030201:Activin Receptors, Type I / Q000235:genetics* / Q000378:metabolism*; D028341:Activins / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D056742:Cyclin D2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D051899:Smad2 Protein / Q000378:metabolism
D020413:3' Untranslated Regions; C486196:Ccnd2 protein, mouse; D056742:Cyclin D2; C543499:MIRN145a microRNA, mouse; D035683:MicroRNAs; D012333:RNA, Messenger; D051899:Smad2 Protein; C497783:Smad2 protein, mouse; C422405:activin A; D028341:Activins; D030201:Activin Receptors, Type I; C498933:Acvr1b protein, mouse