Feasibility study on the use of visible-near-infrared spectroscopy for the screening of individual and total glucosinolate contents in broccoli.
The potential of visible-near-infrared spectroscopy to determine selected individual and total glucosinolates in broccoli has been evaluated. Modified partial least-squares regression was used to develop quantitative models to predict glucosinolate contents. Both the whole spectrum and different spectral regions were separately evaluated to develop the quantitative models; in all cases the best results were obtained using the near-infrared zone between 2000 and 2498 nm. These models have been externally validated for the screening of glucoraphanin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin, and total glucosinolates contents. In addition, discriminant partial least-squares was used to distinguish between two possible broccoli cultivars and showed a high degree of accuracy. In the case of the qualitative analysis, best results were obtained using the whole spectrum (i.e., 400-2498 nm) with a correct classification rate of 100% in external validation being obtained.
Food Colour and Quality Laboratory, Department of Nutrition and Food Science, Facultad de Farmacia, Universidad de Sevilla , 41012 Sevilla, Spain.
José Miguel
JM
Hernández-Hierro
Juan
J
Valverde
Salvador
S
Villacreces
Kim
K
Reilly
Michael
M
Gaffney
Maria Lourdes
ML
González-Miret
Francisco José
FJ
Heredia
Gerard
G
Downey
D001937:Brassica / Q000737:chemistry*; D005240:Feasibility Studies; D005961:Glucosinolates / Q000032:analysis*; D007096:Imidoesters / Q000032:analysis; D007211:Indoles / Q000032:analysis; D016018:Least-Squares Analysis; D010091:Oximes; D064209:Phytochemicals / Q000032:analysis; D015203:Reproducibility of Results; D019265:Spectroscopy, Near-Infrared; D013454:Sulfoxides
D005961:Glucosinolates; D007096:Imidoesters; D007211:Indoles; D010091:Oximes; D064209:Phytochemicals; D013454:Sulfoxides; C403468:methoxyglucobrassicin; C555404:neoglucobrassicin; C048308:glucobrassicin; C119494:glucoraphanin