Cloning and expression of human neuron-specific enolase cDNA in Escherichia coli.
cDNA fragment encoding neuron-specific enolase was amplified from the cDNA library of human brain. Then the fragment was cloned for expression in E. coli using the vector pET28-a. High level of neuron-specific enolase expression was confirmed by SDS-PAAG electrophoresis and immunochemical identity by immunoblot analysis. The constructed producer strain is the cheapest source of neuron-specific enolase suitable for the use in diagnostic applications.
Laboratory of Immunochemistry, Department of Biological Psychiatry, V. P. Serbskii Center of Social and Forensic Psychiatry, Russian State Medical University, Moscow, Russia. pkanew@mail.ru
K A
KA
Pavlov
O I
OI
Gurina
O M
OM
Antonova
A V
AV
Semenova
V P
VP
Chekhonin
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D010751:Phosphopyruvate Hydratase / Q000235:genetics*
D018076:DNA, Complementary; D010751:Phosphopyruvate Hydratase