Decontamination of dental implant surfaces by means of photodynamic therapy.
Several implant surface debridement methods have been reported for the treatment of peri-implantitis, however, some of them can damage the implant surface or promote bacterial resistance. Photodynamic therapy (PDT) is a new treatment option for peri-implantitis. The aim of this in vitro study was to analyze implant surface decontamination by means of PDT. Sixty implants were equally distributed (n = 10) into four groups and two subgroups. In group G1 there was no decontamination, while in G2 decontamination was performed with chlorhexidine. G3 (PDT - laser + dye) and G4 (laser, without dye) were divided into two subgroups each; with PDT performed for 3 min in G3a and G4a, and for 5 min in G3b and G4b. After 5 min in contact with methylene blue dye (G3), the implants were irradiated (G3 and G4) with a low-level laser (GaAlAs, 660 nm, 30 mW) for 3 or 5 min (7.2 and 12 J). After the dilutions, culture media were kept in an anaerobic atmosphere for 1 week, and then colony forming units were counted. There was a significant difference (p < 0.001) between G1 and the other groups, and between G4 in comparison with G2 and G3. Better decontamination was obtained in G2 and G3, with no statistically significant difference between them. The results of this study suggest that photodynamic therapy can be considered an efficient method for reducing bacteria on implant surfaces, whereas laser irradiation without dye was less efficient than PDT.
Department of Prosthodontics and Dental Materials, Medical Faculty, University Hospital RWTH Aachen, Pauwelsstrasse 30, 52074, Aachen, Germany. marotti@usp.br
Juliana
J
Marotti
Pedro
P
Tortamano
Silvana
S
Cai
Martha Simões
MS
Ribeiro
João Eduardo Miranda
JE
Franco
Tomie Toyota
TT
de Campos
D003666:Decontamination / Q000379:methods*; D015921:Dental Implants / Q000382:microbiology*; D006801:Humans; D066298:In Vitro Techniques; D008751:Methylene Blue; D057873:Peri-Implantitis / Q000188:drug therapy / Q000382:microbiology; D010778:Photochemotherapy / Q000379:methods*; D018709:Statistics, Nonparametric; D013499:Surface Properties; D014025:Titanium
D015921:Dental Implants; D014025:Titanium; D008751:Methylene Blue