[Construction of ectoine absorption defective mutant for efficient ectoine production].
OBJECTIVE
To further enhance ectoine (1, 4, 5, 6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) synthesis efficiency.


METHODS
We cloned Halomonas salina DSM 5928(T) teaABC gene of ectoine-specific transporter TeaABC by walking PCR and constructed ectoine absorption defective mutant H. salina DSM 5928(T) (teaABC-) by Red recombination technology.


RESULTS
The total concentration of ectoine and productivity in a 10 L fermentor were 9.10 (+/- 0.08) g/L and 9.93 (+/- 0.09) g/L. d.


CONCLUSIONS
The ectoine absorption defect mutant H. salina DSM 5928(T) (teaABC-) compromised the negative feedback regulation of ectoine synthesis, which can significantly improve the efficiency of ectoine production.
Environmental Science and Engineering College, Dalian Maritime University, Dalian 116026, China. liluodlmu@126.com
Rui
R
Xu
Linghua
L
Zhang
D000327:Adsorption; D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism; D000599:Amino Acids, Diamino / Q000096:biosynthesis*; D005798:Genes, Bacterial; D020632:Halomonas / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation
D026905:Amino Acid Transport Systems; D000599:Amino Acids, Diamino; C045628:ectoine