Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor.
We have reported that polyamines increase cell viability at the stationary phase of cell growth through translational stimulation of ribosome modulation factor, and SpoT and RpoZ proteins involved in the synthesis and function of ppGpp in Escherichia coli. Since biofilm formation is also involved in cell viability, we looked for proteins involved in biofilm formation and cell viability whose synthesis is stimulated by polyamines at the level of translation. It was found that the synthesis of response regulators UvrY and CpxR in the two-component signal transducing systems and ribosome recycling factor (RRF) was increased by polyamines at the level of translation. Polyamine stimulation of the synthesis of UvrY and RRF was dependent on the existence of the inefficient initiation codons UUG and GUG in uvrY and frr mRNA, respectively; and polyamine stimulation of CpxR synthesis was dependent on the existence of an unusual location of a Shine-Dalgarno (SD) sequence in cpxR mRNA. Biofilm formation and cell viability in the absence of polyamines was increased by transformation of modified uvrY and cpxR genes, and cell viability by modified frr gene whose translation occurs effectively without polyamines. The results indicate that polyamines are necessary for both biofilm formation and cell viability.
Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba 288-0025, Japan.
Akihiko
A
Sakamoto
Yusuke
Y
Terui
Taku
T
Yamamoto
Takuma
T
Kasahara
Mizuho
M
Nakamura
Hideyuki
H
Tomitori
Kaneyoshi
K
Yamamoto
Akira
A
Ishihama
Anthony J
AJ
Michael
Kazuei
K
Igarashi
Keiko
K
Kashiwagi
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002942:Circular Dichroism; D003300:Copper / Q000494:pharmacology; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D050296:Microbial Viability / Q000187:drug effects*; D008954:Models, Biological; D008969:Molecular Sequence Data; D011073:Polyamines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013095:Spermidine / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011073:Polyamines; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D014157:Transcription Factors; C419603:UvrY protein, E coli; C087267:ribosome releasing factor; C085504:CpxR protein, Bacteria; D003300:Copper; D013095:Spermidine