Extracellular neutral lipids produced by the marine bacteria Marinobacter sp.
We analyzed the production of neutral lipids by the marine hydrocarbonoclastic bacteria Marinobacter sp. strain PAD-2 using hexadecane or succinate as the sole carbon source. Results showed that strain PAD-2 was able to grow and reduce the surface tension to 33±1.5 mN m(-1) and 58±1.5 mN m(-1) when n-hexadecane or succinate was used as the sole carbon source, respectively. The lipophilic compounds produced by Marinobacter sp. strain PAD-2 were extracted, and then crude lipophilic compounds, expected to be wax ester-like lipids, were analyzed by thin layer chromatography (TLC) . Furthermore, the lipophilic compound demonstrating surface activity was purified and subjected to gas chromatography/mass spectrometry (GC/MS) analysis. Although these did not give definite structural information due to the weak molecular ion peak (M(+)) , one component Ma-1 showed almost the same mass spectrum as that of component Fa-2, which represented a biosurfactant derived from Dietzia maris reported previously. Cell hydrophobicity was measured by a test of bacterial adhesion to hydrocarbons. A higher hydrophobic cell surface was observed in strain PAD-2. Extracellular wax ester-like compounds seem to be one type of the surface active compounds when bacteria grow on hexadecane or succinate as the sole carbon source.
Marine Microbiology, Faculty of Bioresources, Mie University, Mie, Japan. miyo1220_1@hotmail.com
Miyo
M
Nakano
Shunpei
S
Iehata
Reiji
R
Tanaka
Hiroto
H
Maeda
D000473:Alkanes / Q000378:metabolism; D001422:Bacterial Adhesion; D002855:Chromatography, Thin Layer; D004952:Esters / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D005110:Extracellular Space; D008401:Gas Chromatography-Mass Spectrometry; D057927:Hydrophobic and Hydrophilic Interactions; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D053520:Marinobacter / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism; D013500:Surface Tension; D014885:Waxes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D000473:Alkanes; D004952:Esters; D008055:Lipids; D014885:Waxes; D019802:Succinic Acid; C007932:n-hexadecane