Preservation by desiccation of isolated rat hearts for 48 hours using carbon monoxide (PCO = 4,000 hPa) and oxygen (PO(2) = 3,000 hPa).
It is currently said that CO has anti-inflammatory and antiapoptosis effects and it has attracted attention as a medical gas. We used CO for rat hearts and conducted a preservation experiment. We isolated rat hearts, placed them into a specially made chamber, filled the chamber with  a gas mixture of PCO (4,000 hPa) and PO(2) (3,000 hPa), and preserved the hearts in a refrigerator at 4°C for 48 h. We then performed a heterotrophic transplantation on the neck of each recipient rat and resuscitated the preserved hearts. We herein report our findings.
Department of Anatomy, Tokyo Medical University, Shinjuku-ku, Tokyo, Japan. nhatayama416@gmail.com
Naoyuki
N
Hatayama
Munekazu
M
Naito
Shuichi
S
Hirai
Yu
Y
Yoshida
Tomohiro
T
Kojima
Kunihiro
K
Seki
Xiao-Kang
XK
Li
Masahiro
M
Itoh
D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology*; D006085:Graft Survival; D006321:Heart / Q000187:drug effects*; D016027:Heart Transplantation / Q000401:mortality; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D015996:Survival Rate; D013997:Time Factors
D002248:Carbon Monoxide; D010100:Oxygen