Tissue-specific expression of splice variants of human IL-4 and IL-6 gene mRNA.
The spectrum of alternatively spliced IL-4 and IL-6 gene mRNA was studied in peripheral blood mononuclears from healthy donors and in human fetal tissues. It was found that the expression of alternatively spliced IL-4 and IL-6 gene mRNA in fetal tissues is tissue specific and that hemopoiesis- and immunopoiesis-related tissues differ by the amount of IL-4 and IL-4δ2 mRNA. An mRNA variant IL-4alt3 carrying partial exon 3 deletion was for the first time identified in human mononuclear cells.
Research Institute of Clinical Immunology, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia.
O P
OP
Yatsenko
A N
AN
Silkov
E A
EA
Khrapov
M L
ML
Filipenko
V A
VA
Kozlov
S V
SV
Sennikov
D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000096:biosynthesis; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
D017931:DNA Primers; D018076:DNA, Complementary; D015850:Interleukin-6; D020033:Protein Isoforms; D012333:RNA, Messenger; D015847:Interleukin-4