A novel Helicosporium isolate and its antimicrobial and cytotoxic pigment.
One Helicosporium strain, isolated from a wilted chestnut tree, evidenced in vitro antimicrobial activity against various types of bacteria and fungi, and generated a diffusible pigment. The antimicrobial compounds and the diffusible pigment of the Helicosporium sp. isolate were purified via solvent fractionation, column chromatography, and recycling preparative chromatography. Both the major antimicrobial compound and the diffusible pigment were identified as 2-methylresorcinol via nuclear magnetic resonance spectroscopy. Therefore, 2-methylresorcinol, a diffusible pigment generated by Helicosporium sp., appears to be an active antimicrobial principle. This pigment also exhibited considerable cytotoxicity against mammalian cells.
Interdisplinary Program for Biotechnology and Department of Biology, Changwon National University, Changwon, Korea.
Hye Jung
HJ
Choi
Sang Myeong
SM
Lee
Sun-Hee
SH
Kim
Dong Wan
DW
Kim
Young Whan
YW
Choi
Woo Hong
WH
Joo
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D019906:Nuclear Magnetic Resonance, Biomolecular; D010860:Pigments, Biological / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012118:Resorcinols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014197:Trees / Q000382:microbiology
D000900:Anti-Bacterial Agents; D010860:Pigments, Biological; D012118:Resorcinols; C049083:2-methylresorcinol