Water and a protic ionic liquid acted as refolding additives for chemically denatured enzymes.
In this communication, we present the ability of water and a protic ionic liquid, triethyl ammonium phosphate (TEAP) to act as refolding additives for the urea-induced chemical denaturated state of the two enzymes, α-chymotrypsin and succinylated Con A. We show that the enzymatic activity is regained and in certain circumstances enhanced.
Department of Chemistry, University of Delhi, Delhi-110 007, India.
Pankaj
P
Attri
P
P
Venkatesu
Anil
A
Kumar
D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism; D003208:Concanavalin A / Q000737:chemistry* / Q000378:metabolism; D052578:Ionic Liquids / Q000737:chemistry* / Q000494:pharmacology; D010710:Phosphates / Q000737:chemistry*; D011489:Protein Denaturation / Q000187:drug effects; D058849:Protein Refolding / Q000187:drug effects; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D052578:Ionic Liquids; D010710:Phosphates; D000644:Quaternary Ammonium Compounds; D014867:Water; D003208:Concanavalin A; C066585:triethylammonium cation; D002918:Chymotrypsin; C117608:alpha-chymotrypsin