[Role of Rac1 in the SDF-1-induced migration and invasion of human glioma cell line U251].
OBJECTIVE
To explore the role of Rac1 in the SDF-1-induced migration and invasion of glioma cells with a specific Rac1 inhibitor.


METHODS
Human glioma cell lines U251 treated with SDF-1 or/and specific Rac1 inhibitor were used. The migration and invasion capacities of cells in 2D cell migration/3D invasion assay were assessed. Western blot was employed to detect the levels of Rac1 and GAPDH in cell lysates and the Rac1 activity measured by Rac1 activation assays. Immunofluorescence was used to identify the expression and intracellular location of Rac1 in U251 cells.


RESULTS
SDF-1 significantly increased the migration and invasion capacities of U251 cells (P < 0.05). The stimulation of SDF-1 boosted the activity of Rac1 versus the unstimulated cells (P < 0.05). And Rac1 was recruited to protruding edge in SDF-1-stimulated cells. Inhibition of Rac1 with specific Rac1 inhibitor decreased the migration and invasion capacities of SDF-1-induced U251 cells (P < 0.05). In comparison with the SDF-1 treated group, the activity of Rac1 significantly decreased (P < 0.05) and the recruitment of Rac1 to protruding edge significantly decreased in the NSC23766 pre-treated group.


CONCLUSIONS
This study provides novel evidence that Rac1 modulates the SDF-1-induced migration and invasion of glioma cells. It suggests that the inhibition of Rac1 activation may be a new therapeutic target for glioma.
Department of Neurosurgery, Tianjin Medical University General Hospital & Laboratory of Neurooncology, Tianjin Neurological Institute, Tianjin 300052, China.
Bin
B
Zhang
Xue-jun
XJ
Yang
Sheng-ping
SP
Yu
Hao-lang
HL
Ming
Cong
C
Chen
Bing-cheng
BC
Ren
Zhi-feng
ZF
Liu
Bin
B
Liu
D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D009361:Neoplasm Invasiveness; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; C107953:RAC1 protein, human; D020830:rac1 GTP-Binding Protein