Runx3 expression and its roles in mouse endometrial cells.
Runx3 is a transcription factor that belongs to the Runx family. We studied the localization of Runx3 mRNA in the mouse uterus, and its function in the mouse endometrium using Runx3 knockout (Runx3(-/-)) mice. Runx3 mRNA was detected in the endometrial luminal epithelial cells, glandular epithelial cells and stromal cells below the epithelial cell layer on the luminal side. The uteri of Runx3(-/-) mice were smaller than those of wt mice. The endometrial layer and uterine glands of Runx3(-/-) mice were less developed than those of wild-type mice, and the endometrial stromal layer was thinner. Transforming growth factor β1 and β3 (TGFβ1 and β3) mRNA levels in endometrial stromal cells of Runx3(-/-) mice were low compared with those of wild-type mice. Estradiol-17β (E2) increased Tgfb2 mRNA levels in endometrial stromal cells of Runx3(-/-) mice, but not in those of wild-type mice. E2 increased epidermal growth factor (EGF) mRNA levels in endometrial stromal cells of wild-type mice, but did not increase those of Runx3(-/-) mice. The diminished Tgfb1 and Tgfb3 mRNA expressions may lead to the reduced proliferation of endometrial stromal cells. Alterations of E2-associated expressions of Tgfb2 and Egf mRNA in endometrial stromal cells of Runx3(-/-) mice may be associated with suppression of E2-dependent endometrial epithelial cell proliferation in Runx3(-/-) mice. Thus, Runx3 is likely to be a regulatory factor responsible for endometrial growth.
Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
Yukiko
Y
Tsuchiya
Yuka
Y
Saito
Shusuke
S
Taniuchi
Atsuko
A
Sakuma
Tetsuya
T
Maekawa
Hiroshi
H
Fukamachi
Sakae
S
Takeuchi
Sumio
S
Takahashi
D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics / Q000378:metabolism*; D003433:Crosses, Genetic; D004717:Endometrium / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009929:Organ Size; D038081:Organogenesis; D010053:Ovary / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D053782:Transforming Growth Factor beta3 / Q000235:genetics / Q000378:metabolism; D014599:Uterus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
D050677:Core Binding Factor Alpha 3 Subunit; C493730:Runx3 protein, mouse; C508961:Tgfb1 protein, mouse; C509045:Tgfb3 protein, mouse; D053773:Transforming Growth Factor beta1; D053782:Transforming Growth Factor beta3; D004958:Estradiol; D004815:Epidermal Growth Factor