Glycerol monooleate-based nanocarriers for siRNA delivery in vitro.
We present studies of the delivery of short interfering ribonucleic acid (siRNA) into a green fluorescent protein (GFP) expressing cell line, using lipid nanocarriers in cubic lyotropic liquid crystal form. These carriers are based on glycerol monooleate (GMO) and employ the use of varying concentrations of cationic siRNA binding lipids. The essential physicochemical parameters of the cationic lipid/GMO/siRNA complexes such as particle size, ζ otential, siRNA uptake stability, lyotropic mesophase behavior, cytotoxicity,and gene silencing efficiency were systematically assessed. We find that the lipid nanocarriers were effectively taken up by mammalian cells and that their siRNA payload was able to induce gene silencing in vitro. More importantly, it was found that the nonlamellar structure of some of the lipid nanocarrier formulations were more effective at gene silencing than their lamellar structured counterparts. The development of cationic lipid functionalized nonlamellar GMO-based nanostructured nanoparticles may lead to improved siRNA delivery vehicles.
CSIRO Materials Science and Engineering, Bayview Avenue, Clayton, Victoria 3168, Australia. Guoliang.Zhen@gmail.com
Guoliang
G
Zhen
Tracey M
TM
Hinton
Benjamin W
BW
Muir
Shuning
S
Shi
Mark
M
Tizard
Keith M
KM
McLean
Patrick G
PG
Hartley
Pathiraja
P
Gunatillake
D000818:Animals; D016466:CHO Cells; D002412:Cations / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical / Q000379:methods; D006224:Cricetinae; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D020868:Gene Silencing; D005989:Glycerides / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry* / Q000235:genetics
D002412:Cations; D004337:Drug Carriers; D005989:Glycerides; D008055:Lipids; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins; C005953:monoolein