Simple differentiation of avirulent and virulent strains of avian paramyxovirus serotype-1 (Newcastle disease virus) by PCR and restriction endonuclease analysis in Japan.
To differentiate the avirulent from virulent strains of avian paramyxovirus serotype-1 (APMV-1, Newcastle disease virus), PCR and restriction endonuclease analysis (REA) was employed. Primer sequences were used to amplify a 766-base pair fragment that included the fusion protein cleavage site. REA of PCR products generated by Hin1 I and Apa I enabled the differentiation of avirulent field and vaccine strains from more virulent field strains of APMV-1 in Japan.
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Masaji
M
Mase
Katsushi
K
Kanehira
D000818:Animals; D002645:Chickens; D017931:DNA Primers / Q000235:genetics; D062665:Epidemiological Monitoring / Q000662:veterinary; D007564:Japan / Q000453:epidemiology; D009521:Newcastle Disease / Q000453:epidemiology* / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics* / Q000472:pathogenicity*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011201:Poultry Diseases / Q000453:epidemiology* / Q000821:virology*; D015183:Restriction Mapping / Q000379:methods*; D013045:Species Specificity; D014774:Virulence
D017931:DNA Primers