A thin film electro-acoustic enzyme biosensor allowing the detection of trace organophosphorus pesticides.
We report an analytical method using a thin film electro-acoustic resonator for the detection of organophosphorus pesticides. The acetylcholinesterase (AChE) enzyme was immobilized on the surface of the resonator. In the presence of organophosphorus compounds, the degree of inhibitory effect of organophosphorus compounds on the AChE activity and the concentration of pesticides were detected in real time by measuring the frequency shift of the resonator. The proposed device has a remarkably low detection limit of 1.8×10(-11)M and obvious advantages such as small size, simple operation, and integrated circuit compatibility, providing a promising tool for pesticide analysis.
Qingdao Key Laboratory of Terahertz Technology, Department of Physics, School of Science, Shandong University of Science and Technology, Qingdao 266510, People's Republic of China. phychenda@163.com
Da
D
Chen
Jingjing
J
Wang
Yan
Y
Xu
Luyin
L
Zhang
D000110:Acetylcholinesterase / Q000737:chemistry / Q000378:metabolism*; D000162:Acoustics; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000032:analysis*; D010261:Paraoxon / Q000032:analysis; D010575:Pesticides / Q000032:analysis*
D004800:Enzymes, Immobilized; D009943:Organophosphorus Compounds; D010575:Pesticides; D000110:Acetylcholinesterase; D010261:Paraoxon