New naphthalimide modified polyethylenimine nanoparticles as fluorescent probe for DNA detection.
A new naphthalimide modified polyethylenimine (PEI) nanoparticles (called NPEI-NPs) was synthesized and applied as fluorescent probe for rapid, selective and sensitive fluorometric detection of trace DNA. The synthesis involved the covalent modification of PEI with 4-butylamino-N-carboxymethyl-1,8-naphthalimide(BACMN) for getting amphiphilic polymer. Then the amphiphilic polymer was self-assembled in water to give the NPEI-NPs. NPEI-NPs was soluble in water and emitted fluorescence at 545 nm with exciting at 460 nm. The fluorescence spectra resulting from the interaction between NPEI-NPs and DNA indicated that the fluorescence of NPEI-NPs increased in the present of DNA. Therefore, a fluorescence enhancement method was developed for the determination of trace fish sperm DNA (fsDNA) and calf thymus DNA (ctDNA). Under the optimal conditions, the calibration curves were linear over the concentration ranges of 0.05-2.8 μg/mL for fsDNA and 0.08-3.0μg/mL for ctDNA. The detection limits for fsDNA and ctDNA were 1.6 and 2.0 ng/mL, respectively. The proposed method has been employed to quantify DNA in synthetic samples with the satisfactory results.
Institute of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430072, China. chirolab@gmail.com
Shucai
S
Liang
Hui
H
Yu
Jin
J
Xiang
Wang
W
Yang
Xiaohui
X
Chen
Yanbin
Y
Liu
Chen
C
Gao
Guoping
G
Yan
D000818:Animals; D002138:Calibration; D002417:Cattle; D004247:DNA / Q000032:analysis*; D005399:Fishes; D005456:Fluorescent Dyes / Q000737:chemistry*; D008297:Male; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D053644:Naphthalimides / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013094:Spermatozoa / Q000378:metabolism
D005456:Fluorescent Dyes; D053644:Naphthalimides; D011094:Polyethyleneimine; D004247:DNA; C427203:calf thymus DNA