At-line monitoring of key parameters of nisin fermentation by near infrared spectroscopy, chemometric modeling and model improvement.
An analytical procedure has been developed for at-line (fast off-line) monitoring of 4 key parameters including nisin titer (NT), the concentration of reducing sugars, cell concentration and pH during a nisin fermentation process. This procedure is based on near infrared (NIR) spectroscopy and Partial Least Squares (PLS). Samples without any preprocessing were collected at intervals of 1 h during fifteen batch of fermentations. These fermentation processes were implemented in 3 different 5 l fermentors at various conditions. NIR spectra of the samples were collected in 10 min. And then, PLS was used for modeling the relationship between NIR spectra and the key parameters which were determined by reference methods. Monte Carlo Partial Least Squares (MCPLS) was applied to identify the outliers and select the most efficacious methods for preprocessing spectra, wavelengths and the suitable number of latent variables (n (LV)). Then, the optimum models for determining NT, concentration of reducing sugars, cell concentration and pH were established. The correlation coefficients of calibration set (R (c)) were 0.8255, 0.9000, 0.9883 and 0.9581, respectively. These results demonstrated that this method can be successfully applied to at-line monitor of NT, concentration of reducing sugars, cell concentration and pH during nisin fermentation processes.
College of Marine Science, Hainan University, 58 Renmin Road, Haikou, 570228 Hainan, People's Republic of China.
Wei-Liang
WL
Guo
Yi-Ping
YP
Du
Yong-Can
YC
Zhou
Shuang
S
Yang
Jia-Hui
JH
Lu
Hong-Yu
HY
Zhao
Yao
Y
Wang
Li-Rong
LR
Teng
D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D013294:Lactococcus lactis / Q000254:growth & development* / Q000378:metabolism*; D015233:Models, Statistical; D009561:Nisin / Q000378:metabolism*; D019265:Spectroscopy, Near-Infrared / Q000379:methods
D003470:Culture Media; D009561:Nisin