Polymorphisms in the promoter region of the adiponectin (ADIPOQ) gene are presumably associated with transcription level and carcass traits in pigs.
The main goal of this study was to screen for polymorphisms in the porcine adiponectin (ADIPOQ) gene promoter, analyse their influence on transcription and identify any association with production traits in pigs. A 1018-bp region of the ADIPOQ gene promoter was analysed in 113 pigs, and seven novel polymorphisms found. Luciferase assays were performed in HEK293 (human embryonic kidney) cells and primary porcine adipose mesenchymal stem cells (pADMSCs) to investigate their affect on promoter activity. A 16-bp indel (c.-106_-91delGCCAGGGGTGTGAGCC) was found to influence promoter strength in vitro. In the HEK293 cell line, the Del/Del genotype showed greater luciferase activity than did the Ins/Ins genotype (P < 0.01). In pADMSCs, the insertion genotype of the ADIPOQ promoter showed greater luciferase activity than did the deletion genotype (P < 0.01). An association study performed for two novel polymorphisms, c.-67G>A and the 16-bp indel, showed significant correlation with loin measurements in Polish Landrace (P < 0.05) and synthetic line 990 (P < 0.01) pigs.
Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Wolynska 33, 60-637, Poznan, Poland.
J
J
Cieslak
T
T
Flisikowska
A
A
Schnieke
A
A
Kind
M
M
Szydlowski
M
M
Switonski
K
K
Flisikowski
D052242:Adiponectin / Q000235:genetics*; D000818:Animals; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005838:Genotype; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008460:Meat / Q000032:analysis*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D013552:Swine / Q000235:genetics*; D014158:Transcription, Genetic
D052242:Adiponectin; D004247:DNA; D008156:Luciferases