Mutation analysis of NF-κB signal pathway-related genes in ocular MALT lymphoma.
Constitutive nuclear factor-kappa B (NF-κB) activation has been reported in ocular adnexal lymphoma (OAL). TNFAIP3/A20 is a "global" inhibitor of NF-κB pathway. We have shown that OAL has preferential loss of the 6q23.3 region where TNFAIP3/A20 exist, which is suggested to involve in lymphomagenesis of OAL. The mechanisms causing NF-κB activity in OAL remain elusive. Recently, NF-κB canonical pathway genes including CARD11, CD79B and MYD88 were shown to be frequently mutated in diffuse large B-cell lymphomas. In this study, we analyzed the mutation status of these genes by direct sequencing in 24 OAL cases including 9 cases with loss of 6q23.3 previously identified by array comparative genomic hybridization. We showed that genetic alterations of these genes were not found in OAL, a finding differing from that of most B-cell lymphomas. Genetic or epigenetic alterations in other genes are likely to be relevant in pathogenesis of OAL case without A20 loss.
Division of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Japan.
Fang
F
Liu
Kennosuke
K
Karube
Harumi
H
Kato
Kotaro
K
Arita
Noriaki
N
Yoshida
Kiyoko
K
Yamamoto
Shinobu
S
Tsuzuki
Wonseog
W
Kim
Young-Hyeh
YH
Ko
Masao
M
Seto
D053468:CARD Signaling Adaptor Proteins / Q000235:genetics; D051925:CD79 Antigens / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics; D005134:Eye Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006162:Guanylate Cyclase / Q000235:genetics; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008562:Membrane Glycoproteins / Q000235:genetics; D009154:Mutation; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics; D017472:Receptors, Interleukin-1 / Q000235:genetics; D015398:Signal Transduction; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3
D053468:CARD Signaling Adaptor Proteins; D051925:CD79 Antigens; C497883:CD79B protein, human; D017931:DNA Primers; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D009687:Nuclear Proteins; D017472:Receptors, Interleukin-1; C434984:TIRAP protein, human; C076529:TNFAIP3 protein, human; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3; C426242:CARD11 protein, human; D006162:Guanylate Cyclase