Antitumor and immunomodulating activity of a polysaccharide from Sophora flavescens Ait.
The immunostimulatory activity of Sophora flavescens polysaccharide (SFPW1) was evaluated by using in vitro cell models and in vivo animal models. The results demonstrated that SFPW1 could effectively inhibit the tumor growth in H22 tumor-bearing mice and promote the splenocyte proliferation, thus resulting in a prolonged life survival. For assay in vitro, SFPW1 significantly strengthened peritoneal macrophages to devour H22 tumor cells and stimulated macrophages to produce nitric oxide (NO) via up-regulation of inducible NO synthase (iNOS) activity. However, no direct cytotoxicity against H22 tumor cells was observed in vitro. These results suggest that SFPW1 might be a strong natural immunomodulator and the antitumor effect of this polysaccharide is associated with its potent immunostimulating effect.
Gastroenterology Department, The Fourth Hospital of Harbin Medical University, Harbin 150001, China.
Lu
L
Bai
Li-Ying
LY
Zhu
Bao-Shan
BS
Yang
Li-Jun
LJ
Shi
Yong
Y
Liu
Ai-Min
AM
Jiang
Li-Li
LL
Zhao
Guang
G
Song
Tie-Fu
TF
Liu
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012995:Solubility; D029909:Sophora / Q000737:chemistry*; D013154:Spleen / Q000166:cytology; D016019:Survival Analysis; D014867:Water / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D007155:Immunologic Factors; D011134:Polysaccharides; D014867:Water; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II