[Experimental study of Duffy antigen receptor for chemokines in tissue and the inflammatory reaction of limb with venous hypertension].
OBJECTIVE
To explore the role and mechanism of Duffy antigen receptor for chemokines (DARC) of tissue in promoting the inflammatory reaction of the limb with venous hypertension.


METHODS
moral arteriovenous fistula was surgically created to establish the rat model of venous hypertension. A total of 36 SD rats were randomly divided into pcDNA3.1-DARC (Group A), empty plasmid of pcDNA3.1 (Group B) and control (Group C) groups. The animals were sacrificed at Days 14 and 42 post-operation respectively. The expressions of DARC at the RNA and protein level were detected by real-time polymerase chain reaction (PCR) and Western blot. And the serum level of interleukin (IL)-8 was detected by enzyme linked immunosorbent assay (ELISA) and the degrees of apoptosis and leukocytic infiltration of local tissue were detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and hematoxylin and eosin (HE) staining.


RESULTS
With the elapsing time of venous hypertension, the DARC expression in tissue, the extent of apoptosis and leukocytic infiltration in tissue showed an increasing trend in Groups A and B. Group A was obviously higher than Group B during the corresponding period. And the differences were statistically significant (P < 0.05). The serum levels of IL-8 of Groups A and B showed a decreasing trend. And Group A was obviously lower than Group B. Both groups were higher than the control group. The differences were statistically significant (P < 0.05).


CONCLUSIONS
The level of DARC in tissue and the degree of inflammatory reaction of venous hypertension have a positive correlation. And DARC may promote the development of venous hypertension inflammation through augmenting the adhesion and migration of leukocytes.
Department of Vascular Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
Wei-wei
WW
Qi
Lan
L
Zhang
Ji-wei
JW
Zhang
Bai-gen
BG
Zhang
Yi-feng
YF
He
Chun-mei
CM
Chen
Long
L
Yu
D000818:Animals; D004375:Duffy Blood-Group System / Q000276:immunology; D006973:Hypertension / Q000378:metabolism* / Q000503:physiopathology; D016209:Interleukin-8 / Q000097:blood; D010689:Phlebitis / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011946:Receptors, Antigen / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism*; D014690:Venous Pressure
D004375:Duffy Blood-Group System; D016209:Interleukin-8; D011946:Receptors, Antigen; D019707:Receptors, Chemokine