Induction of cellulase and hemicellulase activities of Thermoascus                      aurantiacus by xylan hydrolyzed products.
Thermoascus aurantiacus is able to secrete most of the hemicellulolytic and cellulolytic enzymes. To establish the xylanase inducers of T. aurantiacus, the mycelia were first grown on glucose up until the end of the exponential growth phase, followed by washing and re-suspension in a basal medium without a carbon source. Pre-weighed amounts of xylose (final concentration of 3.5 mg/ml), xylobiose (7 mg/ml) and hydrolyzed xylan from sugarcane bagasse (HXSB) which contained xylose, xylobiose and xylotriose (6.8 mg/ml) were evaluated as inducers of xylanase. It was observed that xylose did not suppress enzyme induction of T. aurantiacus when used in low concentrations, regardless of whether it was inoculated with xylobiose. Xylobiose promoted fast enzyme production stopping after 10 h, even at a low consumption rate of the carbon source; therefore xylobiose appears to be the natural inducer of xylanase. In HXSB only a negligible xylanase activity was determined. Xylose present in HXSB was consumed within the first 10 h while xylobiose was partially hydrolyzed at a slow rate. The profile of α-arabinofuranosidase induction was very similar in media induced with xylobiose or HXSB, but induction with xylose showed some positive effects as well. The production profile for the xylanase was accompanied by low levels of cellulolytic activity. In comparison, growth in HXSB resulted in different profiles of both xylanase and cellulase production, excluding the possibility of xylanase acting as endoglucanases.
Department of Process Engineering, University of Stellenbosch, Private Box X1, Matielend, Stellenbosch, 7602, South Africa. brienzo@sun.ac.za
M
M
Brienzo
J R
JR
Monte
A M F
AM
Milagres
D018533:Biomass; D001709:Biotechnology; D002480:Cellulase / Q000096:biosynthesis*; D004187:Disaccharides / Q000378:metabolism; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000096:biosynthesis*; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D006868:Hydrolysis; D007700:Kinetics; D055327:Thermoascus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D014990:Xylans / Q000378:metabolism
D004187:Disaccharides; D005656:Fungal Proteins; D014990:Xylans; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase; C004173:xylobiose