[Bioconversion of D-fructose to D-allose by novel isomerases].
Rare sugar is a kind of important low-energy monosaccharide that is rarely found in nature and difficult to synthesize chemically. D-allose, a six-carbon aldose, is an important rare sugar with unique physiological functions. It is radical scavenging active and can inhibit cancer cell proliferation. To obtain D-allose, the microorganisms deriving D-psicose 3-epimerase (DPE) and L-rhamnose isomerase (L-RhI) have drawn intense attention. In this paper, DPE from Clostridium cellulolyticum H10 was cloned and expressed in Bacillus subtilis, and L-RhI from Bacillus subtilis 168 was cloned and expressed in Escherichia coli BL21 (DE3). The obtained crude DPE and L-RhI were then purified through a HisTrap HP affinity chromatography column and an anion-exchange chromatography column. The purified DPE and L-RhI were employed for the production of rare sugars at last, in which DPE catalyzed D-fructose into D-psicose while L-RhI converted D-psicose into D-allose. The conversion of D-fructose into D-psicose by DPE was 27.34%, and the conversion of D-psicose into D-allose was 34.64%.
National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Wei
W
Bai
Yueming
Y
Zhu
Yan
Y
Men
Xiaobo
X
Li
Ken
K
Izumori
Yuanxia
Y
Sun
D019747:Aldose-Ketose Isomerases / Q000378:metabolism*; D001412:Bacillus subtilis / Q000201:enzymology; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D046973:Ruminiclostridium cellulolyticum / Q000201:enzymology; D004926:Escherichia coli / Q000378:metabolism; D005632:Fructose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*
D005632:Fructose; C002055:allose; D002238:Carbohydrate Epimerases; C000595678:D-psicose 3-epimerase, Clostridium cellulolyticum; D019747:Aldose-Ketose Isomerases; C062878:L-rhamnose isomerase; D005947:Glucose