[Fabrication of tissue engineered vein containing valve scaffolds].
OBJECTIVE
To fabricate porous biodegradable tissue engineered vein containing valve scaffolds.


METHODS
Based on the self-made cast, the tissue engineered vein containing valve scaffolds was fabricated by injection molding plus thermally induced phase separation. Poly (lactic-co-glycolic acid) (PLGA, LA/GA mole ratio 75:25) was used as matrices. Morphological structures and biocompatibility of scaffolds were tested. Cell seeding on scaffold was performed and the mechanic characteristics of cellular constructs evaluated.


RESULTS
The scaffold had an inner diameter of 9 mm with a wall thickness of 0.9 mm and the thickness of valves was (0.32 ± 0.04) mm. Scanning electron microscopic (SEM) micrographs showed regular ladder-like porous structures and the average pore size and porosity of scaffolds were 10 - 20 µm and 90%. The PLGA scaffolds were biocompatible. The cellular constructs were tested in vitro, and the valve leaflets were functionally capable of opening and closing when stimulated.


CONCLUSIONS
Based on the self-made cast, the tissue engineered vein containing valve scaffolds can be fabricated by injection molding plus thermally induced phase separation. Further researches are warranted.
Division of Vascular Surgery, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
Chi-zhuai
CZ
LIU
Wei-ming
WM
LÜ
Heng-hui
HH
YIN
Chen-guang
CG
ZENG
Da-ping
DP
QUAN
Wen-jian
WJ
Wang
Shen-ming
SM
Wang
D001672:Biocompatible Materials; D019344:Lactic Acid; D008422:Materials Testing; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D055422:Venous Valves
D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid