(1)H NMR-based metabonomic profiling of rat serum and urine to characterize the subacute effects of carbamate insecticide propoxur.
Carbamate insecticide propoxur is widely used in agriculture and public health programs. To prevent adverse health effects arising from exposure to this insecticide, sensitive methods for detection of early stage organismal changes are necessary. We present here an integrative metabonomic approach to investigate toxic effects of pesticide in experimental animals. Results showed that propoxur even at low dose levels can induce oxidative stress, impair liver function, enhance ketogenesis and fatty acid β-oxidation, and increase glycolysis, which contribute to the hepatotoxocity. These findings highlight the applicability of (1)H NMR spectroscopy and multivariate statistics in elucidating the toxic effects of propoxur.
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, PR China.
Yu-Jie
YJ
Liang
Hui-Ping
HP
Wang
Ding-Xin
DX
Long
Yi-Jun
YJ
Wu
D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D007306:Insecticides / Q000494:pharmacology / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics; D015999:Multivariate Analysis; D018384:Oxidative Stress; D010363:Pattern Recognition, Automated; D025341:Principal Component Analysis; D001074:Propoxur / Q000494:pharmacology / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D059551:Toxicity Tests, Subacute
D015415:Biomarkers; D007306:Insecticides; D001074:Propoxur