Role of CD40-dependent signal in induction of recombinase RAG-1 expression in peripheral T cells of patients with autoimmune diabetes mellitus.
We studied the mechanisms of induction of recombinase activity in peripheral T cells of patients with autoimmune type 1 diabetes mellitus. It was shown that the presence of CD40 on T cell membrane (not typical of these cells) is crucial for this process: expression of recombinase RAG-1 in diabetic patients was detected primarily in αβTCR(+)CD40(+) lymphocytes; targeted CD40-dependent activation of intact T cells in vitro increases, while blockade of CD40 signal in the culture of stimulated T cells abolishes recombinase expression.
Institute of Ecology and Genetics of Microorganisms, Ural Division of Russian Academy of Sciences, Perm', Russia. ibis_07@mail.ru
E M
EM
Kuklina
O L
OL
Gorbunova
E N
EN
Smirnova
D000328:Adult; D000911:Antibodies, Monoclonal; D019013:CD40 Antigens / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000276:immunology*; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013601:T-Lymphocytes / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D019013:CD40 Antigens; D018398:Homeodomain Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; C064658:RAG-1 protein