Hepatitis C virus infection modulates expression of interferon stimulatory gene IFITM1 by upregulating miR-130A.
We have examined the underlying mechanism of hepatitis C virus (HCV)-mediated IFITM1 regulation. IFITM1 is a potential target of miR-130a. Our results demonstrated that miR-130a expression was significantly higher in HCV-infected hepatocytes and liver biopsy specimens than in controls. Introduction of anti-miR-130a in hepatocytes increased IFITM1 expression. Hepatocytes stably expressing IFITM1 reduced HCV replication. Together, these results suggested that HCV infection of hepatocytes upregulates miR-130a and that use of anti-miR-130a may have potential for restriction of HCV replication.
Department of Pathology, Saint Louis University, Saint Louis, Missouri, USA.
Joydip
J
Bhanja Chowdhury
Shubham
S
Shrivastava
Robert
R
Steele
Adrian M
AM
Di Bisceglie
Ranjit
R
Ray
Ratna B
RB
Ray
D000943:Antigens, Differentiation / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D055785:Gene Knockdown Techniques; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D006526:Hepatitis C / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D022781:Hepatocytes / Q000276:immunology / Q000821:virology; D006790:Host-Parasite Interactions / Q000235:genetics / Q000276:immunology; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D015854:Up-Regulation; D014779:Virus Replication
D000943:Antigens, Differentiation; C554019:MIRN130 microRNA, human; D035683:MicroRNAs; D012367:RNA, Viral; C062010:leu-13 antigen