Effect of HMG-CoA-reductase inhibitor on DNA synthesis and free radical oxidation in the gastric mucosa under normal conditions and during indometacin-induced ulcerative process in the stomach of albino mice.
We studied the effect of simvastatin (24 mg/kg per os for 30 days) on DNA synthesis ((3)H-thymidine autoradiography) and free radical oxidation (chemiluminescent method) in the gastric mucosa of albino mice under normal conditions and in ulcerative process induced by single indometacin administration. Simvastatin treatment activated free radical oxidation, which was seen from enhanced chemiluminescence in the mucosa homogenate (by 1.7-4.6 times). Administration of indometacin against the background of simvastatin treatment potentiated local oxidative stress and inhibited DNA synthesis. Under these conditions, the area of ulcerative lesion in the gastric mucosa increased by 3.0 times.
Central Research Laboratory of Far Eastern Medical University, Khabarovsk, Russia.
S S
SS
Timoshin
V V
VV
Bragina
O A
OA
Lebedko
E N
EN
Sazonova
E Yu
EY
Zhivotova
M Yu
MY
Fleischman
D000818:Animals; D001345:Autoradiography; D004261:DNA Replication / Q000187:drug effects*; D005609:Free Radicals / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D007213:Indomethacin / Q000009:adverse effects; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D010084:Oxidation-Reduction / Q000187:drug effects; D019821:Simvastatin / Q000494:pharmacology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism*
D005609:Free Radicals; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D019821:Simvastatin; D007213:Indomethacin