Microarray and suppression subtractive hybridization analyses of gene expression in hybrid poplar (Populus alba × Populus tremula var. glandulosa) cell suspension cultures after exposure to NaCl.
The gene expression profiles of hybrid poplar (Populus alba × Populus tremula var. glandulosa) cells in suspension culture after exposure to salinity (NaCl) induced stress were examined by constructing two suppression subtractive hybridization (SSH) libraries. cDNA from non-treated cells was used as a driver and cDNA samples from cell suspension cultures exposed to 150 mM NaCl for 2 or 10 h were used as testers. Randomly selected clones from each SSH library were sequenced and 727 high-quality expressed sequence tags (ESTs) were obtained and analyzed. Four novel ESTs were identified. Between the two libraries, 542 unique SSH clones were selected for placement on a cDNA microarray. In total, 18 differentially expressed genes were identified with 4 and 12 genes being significantly differentially expressed 2 and 10 h after the treatment, respectively. Genes related to metabolism and protein synthesis and several genes whose protein products are implicated in salt or other abiotic stress-related responses were expressed in the salt-stressed cells.
Division of Forest Biotechnology, Korea Forest Research Institute, 39 Onjeong-ro, Suwon 441-847, Republic of Korea.
Eun-Kyung
EK
Bae
Hyoshin
H
Lee
Jae-Soon
JS
Lee
Eun-Woon
EW
Noh
Young-Im
YI
Choi
Byung-Hyun
BH
Lee
Dong-Woog
DW
Choi
D018929:Cell Culture Techniques; D020224:Expressed Sequence Tags; D015870:Gene Expression / Q000187:drug effects*; D015723:Gene Library; D017343:Genes, Plant; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D032107:Populus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D055049:Salt Tolerance / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000235:genetics*
D010940:Plant Proteins; D012965:Sodium Chloride