Backbone modification promotes peroxidase activity of G-quadruplex-based DNAzyme.
In this work we studied how backbone chemical modifications, such as 2'-O-methyl, phosphorothioate, L-form nucleotides and locked nucleic acid, on G-quadruplex based DNAzymes would affect their peroxidase activity. Our results indicate that 2'-O-methyl modification facilitates the formation of a perfectly compacted parallel structure and significantly promotes peroxidase activity of G-quadruplex based DNAzymes.
State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Cong
C
Li
Ling
L
Zhu
Zhi
Z
Zhu
Hao
H
Fu
Gareth
G
Jenkins
Chunming
C
Wang
Yuan
Y
Zou
Xin
X
Lu
Chaoyong James
CJ
Yang
D001665:Binding Sites; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D004789:Enzyme Activation; D004795:Enzyme Stability; D054856:G-Quadruplexes; D008958:Models, Molecular; D015394:Molecular Structure; D010544:Peroxidases / Q000378:metabolism*
D021881:DNA, Catalytic; D010544:Peroxidases