Biological and molecular characterization of a U.S. isolate of Hosta virus X.
Hosta virus X (HVX) is rapidly becoming a serious pathogen of commercially important hosta plants worldwide. We report here biological and molecular characterization of a U.S. isolate of HVX, HVX-37. HVX-37 infectivity was tested in 21 hosta cultivars over three growth seasons, and three types of responses were defined based upon the ability of the virus to cause local and/or systemic infections. Four cultivars resistant to systemic HVX infection were identified. The full-length sequence of the HVX-37 genome was determined, the first complete sequence of a U.S. HVX isolate. Comparison with the previously sequenced HVX-Korea (Kr) genome revealed a high level of sequence similarity, as well as some differences. Notably, a 105-nucleotide long, near-perfect direct repeat in the Kr isolate is absent in HVX-37. The accuracy of the HVX-37 genome sequence was confirmed by infectivity of in vitro transcripts synthesized from a full-length HVX-37 cDNA on Nicotiana benthamiana and hosta plants.
Department of Plant Pathology, The Ohio State University, Columbus 43210, USA. de-la-torre-cuba.1@buckeyemail.osu.edu
Carola M
CM
De La Torre
Feng
F
Qu
Margaret G
MG
Redinbaugh
Dennis J
DJ
Lewandowski
D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D031422:Hosta / Q000821:virology*; D058974:Phylogeography; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics*; D012367:RNA, Viral; D014481:United States
D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral