Morphine-induced place preference affects mRNA expression of G protein α subunits in rat brain.
BACKGROUND
The conditioned place preference (CPP) test is an animal model serving to assess addictive potential of drugs in which environmental cues become associated with the subjective effects of drugs of abuse. Morphine, a known addictive drug, is an agonist of opioid receptors that couple to the G(i/o) family of guanine nucleotide-binding proteins (GP). We have recently found that chronic treatment with morphine affects mRNA levels of GPs that are not coupled to opioid receptors (OR). Therefore, in this study, we investigated the influence of morphine-induced CPP on mRNA expression of the Gα subunits, G(i/o), G(s), G(q/11), and G(12), in the rat prefrontal cortex (PFC) and nucleus accumbens (NAc) using standard PCR techniques.


METHODS
CPP and NO-CPP experiments were conducted; Wistar rats were either subjected to the standard CPP procedure or were injected with morphine (or saline) in their home cage. All rats were decapitated 24 h after the last injection.


RESULTS
We found that mRNA levels of Gα(q), Gα(11) and Gα(12) were increased after morphine in non-conditioned treatment in the PFC but remained unchanged in the NAc. In rats showing conditioned place preference to morphine, levels of Gα(i2) in the PFC and levels of Gα(oA) in the NAc were diminished by ≈58% and ≈30%, respectively (p < 0.05 vs. saline), but levels of Gα(s-l) in NAc were increased (≈60%, p = 0.05).


CONCLUSIONS
Our data indicate that only G(i/o) and G(s) were specifically changed in animals after morphine-induced CPP, thus suggesting that the effect was related to learning environmental cues associated with morphine.
Department of Brain Biochemistry, Institute of Pharmacology, Polish Academy of Science, Smętna 12, PL 31-343 Kraków, Poland.
Agnieszka
A
Zelek-Molik
Adam
A
Bielawski
Grzegorz
G
Kreiner
Piotr
P
Popik
Jerzy
J
Vetulani
Irena
I
Nalepa
D000701:Analgesics, Opioid / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D003213:Conditioning, Psychological / Q000187:drug effects; D003463:Cues; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D009020:Morphine / Q000494:pharmacology*; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar
D000701:Analgesics, Opioid; D044385:GTP-Binding Protein alpha Subunits; D012333:RNA, Messenger; D009020:Morphine