[Antibacterial mechanism of sulforaphane on Escherichia coli].
OBJECTIVE
To investigate the antibacterial mechanism of sulforaphaneon (SFN) on Escherichia coli.


METHODS
To determine membrane penetrability, changes of SDS-PAGE protein spectra, soluble protein and alkaline phosphatase and reducing sugar were determined. Cellular nucleic acid synthesis was detected by 4, 6- diamidino-2-phenylindole (DAPI) staining assay.


RESULTS
SFN affected the membrane permeability of Escherichia coli. Ions and small molecules could leak out of the cells. But it did not destroy the membrane integrity directly. After 16 hours of treatment with SFN, the total contents of intracellular and extracellular proteins decreased by 42.5% and 17.6%, respectively, while the quantity of DNA and RNA reduced by 34.8% and 48.5% respectively.


CONCLUSIONS
SFN can affect cell membrane permeability, material and energy metabolism and inhibit the synthesis of nucleic acid and protein.
Department of Bioscience, Bengbu Medical College, Bengbu 233030, China.
Hua-Zhang
HZ
Wu
Hong-Jun
HJ
Fei
Yun-li
YL
Zhao
Xiao-jia
XJ
Liu
Yin-jiu
YJ
Huang
Shou-wei
SW
Wu
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects*; D017879:Isothiocyanates; D013454:Sulfoxides; D013861:Thiocyanates / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D017879:Isothiocyanates; D013454:Sulfoxides; D013861:Thiocyanates; C016766:sulforaphane