[Changes of prostaglandin D2,carboxypeptidase A3 and platelet activating factor in guinea pig in anaphylactic shock].
OBJECTIVE
To detect the changes of leukotriene E4(LTE4), prostaglandin D2(PGD2), carboxypeptidase A3(CPA3) and platelet activating factor (PAF) in guinea pigs died from anaphylactic shock.


METHODS
Guinea pigs were used for establishing anaphylactic shock models. The levels of LTE4, PGD2 and CPA3, and PAF were detected in urine, plasma, and brain tissues with ELISA kit, respectively. The significant biomarkers were selected comparing with control group. The changes of PGD2, CPA3 and PAF in the guinea pigs at time zero, 12 and 24 hours after death were observed and compared respectively. The effect of platelet activating factor acetylhydrolase (PAF-AH) to PAF in guinea pig brain was examined and compared.


RESULTS
There were no statistically differences of LTE4 levels in urine observed between experimental group and control group. The levels of CPA3, PGD2 and PAF in the experimental group were significantly higher than that in the control group at 0 h. The levels of PAF at 12 and 24 hours after anaphylactic shock were significantly higher than that in the control group. The levels of PAF decreased significantly after pretreatment with PAF-AH.


CONCLUSIONS
LTE4 in urine cannot be selected as a biomarker to determine the anaphylactic shock. PGD2 and CPA3 in plasma, and PAF in brain tissue may be used as biomarkers to determine the anaphylactic shock. PAF-AH may be potentially useful for clinical treatment of anaphylactic shock.
Department of Forensic Pathology, School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. mryangk@163.com
Kai
K
Yang
Xiang-jie
XJ
Guo
Xue-bin
XB
Yan
Cai-rong
CR
Gao
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000008:administration & dosage / Q000494:pharmacology; D000707:Anaphylaxis / Q000097:blood / Q000175:diagnosis* / Q000517:prevention & control; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002268:Carboxypeptidases / Q000097:blood*; D016022:Case-Control Studies; D004195:Disease Models, Animal; D004527:Egg Proteins / Q000008:administration & dosage; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006168:Guinea Pigs; D017999:Leukotriene E4 / Q000652:urine; D008297:Male; D051379:Mice; D010972:Platelet Activating Factor / Q000187:drug effects / Q000378:metabolism*; D015230:Prostaglandin D2 / Q000097:blood*; D013997:Time Factors
D004527:Egg Proteins; D010972:Platelet Activating Factor; D017999:Leukotriene E4; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D002268:Carboxypeptidases; D015230:Prostaglandin D2