Suppression of feline calicivirus replication using small interfering RNA targeted to its polymerase gene.
Feline calicivirus  (FCV)  is a pathogenic microorganism that causes upper respiratory diseases in cats. Recently, an FCV infection with a high mortality rate has been confirmed, and there is need to develop a treatment for cases of acute infection. We evaluated whether the replication of FCV could be prevented by RNA interference.  For this study, we designed an siRNA targeted to the polymerase region of the strain FCV-B isolated from a cat that died after exhibiting neurological symptoms. Cells transfected with siR-pol dose-dependently suppressed the replication of FCV-B. siR-pol suppressed its replication by suppressing the target viral RNA.
Department of Microbiology II, School of Veterinary Medicine, Azabu University, Kanagawa, Japan. tsatoshi@azabu-u.ac.jp
Satoshi
S
Taharaguchi
Takahisa
T
Matsuhiro
Hayato
H
Harima
Atsuko
A
Sato
Kyoko
K
Ohe
Sachi
S
Sakai
Toshikazu
T
Takahashi
Motonobu
M
Hara
D000818:Animals; D017250:Caliciviridae Infections / Q000628:therapy / Q000662:veterinary* / Q000821:virology; D017927:Calicivirus, Feline / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D002371:Cat Diseases / Q000401:mortality / Q000628:therapy / Q000821:virology*; D002415:Cats; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D015751:Genes, pol / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D013997:Time Factors; D014162:Transfection / Q000662:veterinary; D014779:Virus Replication / Q000235:genetics*
D034741:RNA, Small Interfering; D012367:RNA, Viral