Adaptation of glycolysis and growth to acetate in Sporolactobacillus sp. Y2-8.
Exogenous addition of a low concentration of acetate (2 g/L sodium acetate) effectively decreased acetic acid excretion and lowered the ATP content in Sporolactobacillus sp. Y2-8 without any growth defect although the acetate could not be utilized at an initial glucose concentration of 150 g/L. This induced an enhanced glycolytic flux with increased specific activities of hexokinase and phosphofructokinase, probably to compensate for the lowered efficiency of ATP production. However, with increasing concentrations (5 g/L sodium acetate), acetate was utilized first before being produced again, causing a growth lag at the transition. Glucose consumption was also reduced at high acetate concentrations, resulting in decreased D-lactic acid production. These results demonstrate that acetate plays a significant role in regulating glycolysis and growth of Sporolactobacillus.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, Peoples' Republic of China.
Dong
D
Liu
Yong
Y
Chen
An
A
Li
Ting
T
Zhao
Tao
T
Zhou
Bingbing
B
Li
Jingjing
J
Xie
Xiaochun
X
Chen
Jianxin
J
Bai
Yanan
Y
Hu
Bingfang
B
He
Hanjie
H
Ying
D000085:Acetates / Q000378:metabolism / Q000494:pharmacology*; D000222:Adaptation, Physiological / Q000187:drug effects*; D000255:Adenosine Triphosphate / Q000378:metabolism; D056449:Bacillales / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis
D000085:Acetates; D019344:Lactic Acid; D000255:Adenosine Triphosphate