The effects of chemokine, adhesion and extracellular matrix molecules on binding of mesenchymal stromal cells to poly(l-lactic acid).
OBJECTIVE
Mesenchymal stromal cells (MSC) are pluripotent adult stem cells capable of osteogenesis and chondrogenesis to form bone and cartilage. This characteristic gives them the potential for bone and cartilage regeneration. Synthetic polymers have been studied to examine whether they could be used as a scaffold for tissue engineering. In the current study a two-dimensional (2-D) poly(l-lactic acid) (PLLA) scaffold was treated with chemokine, adhesion and extracellular matrix molecules with the aim of using biologic molecules to improve the attachment of human MSC.


METHODS
MSC were isolated from human bone marrow and applied to a 2-D PLLA scaffold. Chemokines ligand (CXCL12 and CXCL13), adhesion molecules [P-selectin, vascular cell adhesion molecule (VCAM)-1 and heparin] and extracellular matrix molecules (fibronectin and type IV collagen) were coated on the scaffold and their effects on the number of MSC that adhered were recorded.


RESULTS
When used alone CXCL12 and CXCL13 enhanced MSC adhesion, as did VCAM-1, P-selectin, fibronectin and collagen, but not heparin. The effects of VCAM-1, P-selectin and heparin were enhanced by the addition of CXCL12. Incubation of MSC with antibodies to integrins α4 and α5β1 inhibited their adhesion to VCAM-1 and fibronectin-treated PLLA respectively, suggesting that these integrins were involved in the MSC interactions.


CONCLUSIONS
The use of certain chemokines and adhesion and extracellular matrix molecules, alone or in combination, is beneficial for the attachment of MSC to PLLA, and may be helpful as natural molecules in scaffolds for regenerative medicine.
Leopold Muller Arthritis Research Centre, Institute for Science and Technology in Medicine, Medical School, Keele University, RJAH Orthopaedic Hospital, Oswestry, UK.
Sylvia
S
Weeks
Anirudh
A
Kulkarni
Helen
H
Smith
Catherine
C
Whittall
Ying
Y
Yang
Jim
J
Middleton
D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000166:cytology; D001861:Bone Regeneration; D002356:Cartilage; D002448:Cell Adhesion; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000737:chemistry; D054382:Chemokine CXCL13 / Q000737:chemistry; D024141:Collagen Type IV / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry; D005260:Female; D005353:Fibronectins / Q000737:chemistry; D006493:Heparin / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology; D008875:Middle Aged; D019007:P-Selectin / Q000737:chemistry; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D044968:Regenerative Medicine; D023822:Tissue Engineering; D019010:Vascular Cell Adhesion Molecule-1 / Q000737:chemistry
D054377:Chemokine CXCL12; D054382:Chemokine CXCL13; D024141:Collagen Type IV; D005353:Fibronectins; D019007:P-Selectin; D011091:Polyesters; D011108:Polymers; D019010:Vascular Cell Adhesion Molecule-1; D019344:Lactic Acid; C033616:poly(lactide); D006493:Heparin