Chromatographic resolution and isotherm determination of (R,S)-mandelic acid on Chiralcel-OD column.
Resolution of racemic mandelic acid ((R,S)-MA) and numerical determination of binary competitive isotherm of (R,S)-MA on Chiralcel-OD column have been investigated in this study. The effects of the alcohol modifier and acidic additive in the mobile phase on the retention and enantioseparation of (R,S)-MA were studied at first. The inverse method was then used to determine the competitive isotherm parameters of (R,S)-MA by minimizing the sum of square deviations of the model predictions from the measured elution profiles. The results indicate that the mobile phase with 85% hexane/15% isopropanol/0.3% trifluoroacetic acid mixture gives the best resolution of (R,S)-MA and competitive-modified Langmuir isotherm provides the more accurate sorption mechanism of (R,S)-MA on the cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase.
Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Canada.
Shimin
S
Mao
Yan
Y
Zhang
Sohrab
S
Rohani
Ajay K
AK
Ray
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D008333:Mandelic Acids / Q000737:chemistry*; D013237:Stereoisomerism
D008333:Mandelic Acids; C037938:mandelic acid