[Primary safety evaluation of sulfated paeoniae radix alba].
The paper is to report the development of a method of quantitative analysis of multi-components by high performance liquid chromatography (HPLC) for simultaneously determining paeoniflorin sulfonate (PS), paeoniflorin (PF) and albiflorin (AF) in sulfated Paeoniae Radix Alba. Moreover, the cytotoxicity of paeoniflorin sulfonate by MTT-assay and the acute toxicity of mice by administration of paeoniflorin sulfonate were evaluated. Chromatographic separation of paeoniflorin sulfonate, PF and AF were performed on a SHISEIDO CAPCELL PAK C18 column (250 mm x 4.6 mm, 5 microm) for HPLC and a mixture of acetonitrile and 0.02% phosphoric acid solution (15 : 85) as the mobile phase. As detector a spectrophotometer set at 230 nm; column temperature 30 degrees C; flow rate 1.0 mL x min(-1). The toxicity of paeoniflorin sulfonate was evaluated by in vitro cytotoxicity carried out on mouse and human primary hepatocytes, and by acute oral toxicity test carried out on mice. The calibration curve of paeoniflorin sulfonate, PF and AF revealed linearity in the range of 0.041 8 - 1.045 0, 0.023 5 - 0.587 5, and 0.039 8 - 0.995 0 mg x mL(-1), respectively (r > 0.999 8). The average recovery was ranged from 99.11% to 101.71%, RSD < 2%. Paeoniflorin sulfonate does not have any cytotoxicity to cells at all the tested concentrations (< or = 300 micromol x L(-1)) in the in vitro cytotoxicity assay. The maximum tolerance dose of paeoniflorin sulfonate solution and extraction of Paeoniae Radix Alba to mouse is 5 g x kg(-1) and 80 g x kg(-1) respectively. The contents of these three components in the samples were determined with the developed method. It is a rapid, convenient and accurate method to determine multi-components. The content of PF in sulfated Paeoniae Radix Alba is significantly lower, and there is negative correlationship between the content of paeoniflorin sulfonate and PF. The in vitro cytotoxicity assay and in vivo mouse acute toxicity test showed that there is no obvious toxicity of paeoniflorin sulfonate and water-soluble extract of sulfated Paeoniae Radix Alba.
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Shan-Jun
SJ
Huang
Rui
R
Wang
Yan-Hong
YH
Shi
Li
L
Yang
Zai-Yong
ZY
Wang
Zheng-Tao
ZT
Wang
D000818:Animals; D001565:Benzoates / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*; D001952:Bridged-Ring Compounds / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry* / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry / Q000187:drug effects; D013455:Sulfur / Q000494:pharmacology; D014835:Volatilization; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D039821:Monoterpenes; D013455:Sulfur; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin