Lung infection caused by Mycobacterium riyadhense confused with Mycobacterium tuberculosis: the first case in Korea.
A slowly growing, non-chromogenic mycobacterial strain was isolated from sputum and bronchial lavage fluid samples of a patient presenting with productive cough, blood-tinged sputum, low-grade fever, and weakness. A positive acid-fast bacilli sputum smear result prompted the initiation of an anti-tuberculosis regimen. Multiplex real-time PCR showed a negative result for Mycobacterium tuberculosis complex and a positive result for nontuberculous mycobacteria. The DNA chip test confirmed this organism as a member of the genus Mycobacterium, but could not specify the species. Interestingly, the mycolic acid patterns obtained by HPLC nearly overlapped with those of M. simulans. The sequences of the Mycobacterium 16S rRNA gene and 16S-23S internal transcribed spacer region were unique and were found to have 100% similarity with those of M. riyadhense. After a review of the literature, we report this case as the first Korean case of M. riyadhense lung infection.
Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Korea.
Jung-In
JI
Choi
Ji-Hun
JH
Lim
Sung-Ryul
SR
Kim
Seon Ho
SH
Lee
Jae-Sun
JS
Park
Kwang Won
KW
Seo
Jae Bum
JB
Jeon
Joseph
J
Jeong
D000328:Adult; D000995:Antitubercular Agents / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D009171:Mycolic Acids / Q000032:analysis; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
D000995:Antitubercular Agents; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S