Differential proteomic analysis of a polymicrobial biofilm.
Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia exist in a polymicrobial biofilm associated with chronic periodontitis. The aim of this study was to culture these three species as a polymicrobial biofilm and to determine proteins important for bacterial interactions. In a flow cell all three species attached and grew as a biofilm; however, after 90 h of culture P. gingivalis and T. denticola were closely associated and dominated the polymicrobial biofilm. For comparison, planktonic cultures of P. gingivalis and T. denticola were grown separately in continuous culture. Whole cell lysates were subjected to SDS-PAGE, followed by in-gel proteolytic H₂¹⁶O/H₂¹⁸O labeling. From two replicates, 135 and 174 P. gingivalis proteins and 134 and 194 T. denticola proteins were quantified by LC-MALDI TOF/TOF MS. The results suggest a change of strategy in iron acquisition by P. gingivalis due to large increases in the abundance of HusA and HusB in the polymicrobial biofilm while HmuY and other iron/haem transport systems decreased. Significant changes in the abundance of peptidases and enzymes involved in glutamate and glycine catabolism suggest syntrophy. These data indicate an intimate association between P. gingivalis and T. denticola in a biofilm that may play a role in disease pathogenesis.
Oral Health CRC, Melbourne Dental School and the Bio21 Institute, The University of Melbourne, Victoria 3010, Australia.
Zamirah
Z
Zainal-Abidin
Paul D
PD
Veith
Stuart G
SG
Dashper
Ying
Y
Zhu
Deanne V
DV
Catmull
Yu-Yen
YY
Chen
Deasy C
DC
Heryanto
Dina
D
Chen
James S
JS
Pyke
Kheng
K
Tan
Helen L
HL
Mitchell
Eric C
EC
Reynolds
D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D041963:Bacteroidetes / Q000737:chemistry / Q000502:physiology; D018441:Biofilms; D002853:Chromatography, Liquid; D059013:Microbial Consortia; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000502:physiology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D048373:Treponema denticola / Q000737:chemistry / Q000502:physiology
D001426:Bacterial Proteins; D020543:Proteome