Association of age-related changes in circulating intermediary lipid metabolites, inflammatory and oxidative stress markers, and arterial stiffness in middle-aged men.
The relationships between age-related changes in circulating endogenous metabolites, inflammatory and oxidative stress markers, and arterial stiffness in 57 middle-aged (34-55 years), nonobese men were studied over the course of 3 years. Arterial stiffness was measured using brachial-ankle pulse wave velocities (ba-PWV). Plasma metabolomic profiling was performed using ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry. After 3 years, decreased HDL cholesterol and increased malondialdehyde (MDA) and ox-LDL levels were observed. Among 15 identified lipids, lysoPCs (C16:0, C18:0, C18:2, C20:4, and C20:5) and linoleyl carnitine were the major plasma metabolites that contributed to the age-related differences. LysoPC16:0 (variable importance in the projection value, 6.2029) was found as the most important plasma metabolite for evaluating these changes. Changes in lysoPC16:0 levels positively correlated with the changes in 8-epi-PGF2α (r = 0.608), MDA (r = 0.413), high-sensitivity C-reactive protein (r = 0.509), IL-6 (r = 0.497), and ba-PWV (r = 0.283) levels. ba-PWV levels positively correlated with the changes in waist-to-hip ratios (WHR), inflammatory and oxidative stress markers. In a subgroup analysis of subjects with decreased ba-PWVs vs. increased ba-PWVs, changes in WHR and levels of lysoPC16:0, ba-PWV, IL-6, 8-epi-PGF2α, MDA, and P-selectin were significantly different. Our results suggest that age-related increases in lysoPC16:0 may contribute to lipid peroxidation, thereby activating proinflammatory phenotypes and arterial stiffness.
Yonsei University Research Institute of Science for Aging, Yonsei University, Seoul, Korea.
Ji Young
JY
Kim
Oh Yoen
OY
Kim
Jean Kyung
JK
Paik
Dae Young
DY
Kwon
Hyun-Jin
HJ
Kim
Jong Ho
JH
Lee
D000328:Adult; D000367:Age Factors; D000375:Aging / Q000502:physiology*; D055109:Ankle Brachial Index; D015415:Biomarkers / Q000097:blood*; D001783:Blood Flow Velocity; D002339:Carotid Arteries / Q000503:physiopathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000453:epidemiology / Q000503:physiopathology; D050356:Lipid Metabolism / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D015995:Prevalence; D056910:Republic of Korea / Q000453:epidemiology; D012189:Retrospective Studies; D059289:Vascular Stiffness / Q000502:physiology*; D049629:Waist-Hip Ratio
D015415:Biomarkers