Removal of crude oil by microbial consortium isolated from oil-spilled area in the Korean Western coast.
The feasibility of using an indigenous microbial consortium for the removal of crude oil from an oil-spilled coastal area was explored with the ultimate aim of applying for bioremediation. Initially, we obtained the microbial consortium TK-2 that catalyzed the dispersion as well as the degradation of crude oil in supplemented sea water. GC and GC-MS were used to evaluate the removal patterns of crude oil during the incubation. The effective removal of crude oil by TK-2 occurred, and above 95% of all aliphatic and aromatic compounds detected in this work was removed within 30 days of incubation. Two predominant crude oil-grown isolates derived from TK-2 revealed gram-negative, rod-shaped cells. Both BIOLOG system and 16S rRNA sequencing were conducted to identify the strains, which were assigned to Arthrobacter sp. HK-2 and Pseudoalteromonas sp. HK-3, and registered in GenBank as [FJ477042] and [FJ477041].
Department of Life Science and Biotechnology, Soonchunhyang University, P.O.Box 97, Asan, Chung-Nam, 336-600, Republic of Korea.
Su-Hee
SH
Cho
Kye-Heon
KH
Oh
D001419:Bacteria / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D001483:Base Sequence; D055162:Biocatalysis; D001673:Biodegradation, Environmental; D017931:DNA Primers; D008401:Gas Chromatography-Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010578:Petroleum; D059392:Petroleum Pollution; D016133:Polymerase Chain Reaction; D056910:Republic of Korea
D017931:DNA Primers; D010578:Petroleum