Improved burns therapy: liposomes-in-hydrogel delivery system for mupirocin.
Wounds, particularly burns, are prone to colonization of potentially life-threatening bacteria. Local delivery of antimicrobial agents in sufficient quantities and over longer period of time can reduce risk of burn infections. Mupirocin-in-liposomes-in-hydrogels were proposed as advanced delivery system for improved burn therapy. Mupirocin was entrapped in phosphatidylcholine liposomes of various sizes, namely larger (micron size) vesicles entrapping 74% of drug and sonicated vesicles (below 300 nm) entrapping 49% of drug. Liposomes containing mupirocin were incorporated in chitosan hydrogels (10%, w/w). Incorporation of liposomes in hydrogels resulted in prolonged release of liposomally associated mupirocin, as observed in both in vitro and ex vivo studies. The drug release was affected by the vesicle size. Microbiological evaluation of newly developed system confirmed its antimicrobial potential against Staphylococcus aureus and Bacillus subtilis. Bioadhesiveness of the system was compared with the marketed cream containing mupirocin. Our system exhibited superior bioadhesiveness and sustained mupirocin release profiles to marketed product.
Drug Transport and Delivery Research Group, Department of Pharmacy, Faculty of Health Sciences, University of Tromsø, Tromsø N-9037, Norway.
Julia
J
Hurler
Ole Aleksander
OA
Berg
Merete
M
Skar
Anne Hilde
AH
Conradi
Pål Jarle
PJ
Johnsen
Nataša
N
Skalko-Basnet
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D001412:Bacillus subtilis / Q000187:drug effects; D002056:Burns / Q000188:drug therapy*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage* / Q000737:chemistry*; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry*; D016712:Mupirocin / Q000008:administration & dosage* / Q000737:chemistry*; D010316:Particle Size; D010713:Phosphatidylcholines / Q000008:administration & dosage; D012867:Skin / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects; D013552:Swine; D014946:Wound Infection / Q000188:drug therapy / Q000517:prevention & control
D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D008081:Liposomes; D010713:Phosphatidylcholines; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan; D016712:Mupirocin