A well-defined block copolymer serving as surfactants in separation of 1,4-dihydropyridines by open-tubular capillary eletrochromatography.
A novel series of diblock copolymers, poly(butyl methacrylate)(n) -block-poly(glycidyl methacrylate)(m) [P(BMA)(n) -b-P(GMA)(m) ], were synthesized by atom transfer radical polymerization and developed as covalent coating of capillaries. The excellent performance of this coating in separation of three 1,4-dihydropyridines (DHPs) derivatives (amlodipine, nicardipine, nitrendipine) was achieved when the diblock copolymers self-assembled into micelles, which was confirmed by transmission electron microscopy, dynamic light scattering, and atom force microscopy. Meanwhile, the effects of block ratio n/m, pH value, buffer concentration, and organic solvents on the separation of 1,4-DHPs were investigated in detail. Then, the relationship between the morphologies of copolymers and the separation resolutions of 1,4-DHPs was discussed. Furthermore, the proposed method exhibited good run-to-run and column-to-column precision with relative standard deviations of electroosmotic flow less than 3.0%. It was also validated with linearity of three 1,4-DHPs in the range of 0.01-1.80 mM (r(2) ≥ 99.7%), efficient recovery (94-103%), and good repeatability (≤ 3.8%). In addition, three 1,4-DHPs were successfully separated in the spiked human serum sample, which indicated the potential utility of this method in biological sample analysis.
Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
Yaping
Y
Li
Li
L
Qi
Juan
J
Qiao
Ying
Y
Shen
Huijuan
H
Yan
Peiyong
P
Xin
Huimin
H
Ma
D000097:Acetonitriles / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000379:methods*; D004095:Dihydropyridines / Q000097:blood / Q000302:isolation & purification*; D005663:Furans / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D011109:Polymethacrylic Acids / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013501:Surface-Active Agents / Q000737:chemistry*
D000097:Acetonitriles; D004095:Dihydropyridines; D005663:Furans; D011109:Polymethacrylic Acids; D013501:Surface-Active Agents; C042535:polyglycidyl methacrylate; C018674:tetrahydrofuran; C068872:polybutyl methacrylate; C032159:acetonitrile