Identification of HLA-A24-restricted novel T Cell epitope peptides derived from P-cadherin and kinesin family member 20A.
We here identified human leukocyte antigen-(HLA-)A(∗)2402-restricted epitope peptides from Cadherin 3, type 1, P-cadherin (CDH3) and kinesin family member 20A (KIF20A) that were found to be specifically expressed in cancer cells through genome-wide expression profile analysis. CDH3-10-807 peptide and KIF20A-10-66 peptide successfully induced specific CTL clones, and these selectively responded to COS7 cells expressing both HLA-A(∗)2402 and respective protein while did not respond to parental cells or COS7 cells expressing either HLA-A(∗)2402 or respective protein. Furthermore, CTL clones responded to cancer cells that endogenously express HLA-A(∗)2402 and respective protein, suggesting that CDH3-10-807 peptide and KIF20A-10-66 peptide are naturally presented on HLA-A(∗)2402 molecule of human cancer cells. Our results demonstrated that CDH3-10-807 peptide and KIF20A-10-66 peptide are novel HLA-A24-restricted tumor-associated antigens and would be applicable for CTL-inducing cancer therapies.
Second Department of Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8510, Japan.
Ryuji
R
Osawa
Takuya
T
Tsunoda
Sachiko
S
Yoshimura
Tomohisa
T
Watanabe
Motoki
M
Miyazawa
Masaji
M
Tani
Kazuyoshi
K
Takeda
Hidewaki
H
Nakagawa
Yusuke
Y
Nakamura
Hiroki
H
Yamaue
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D015820:Cadherins / Q000737:chemistry / Q000276:immunology*; D002460:Cell Line; D002470:Cell Survival / Q000276:immunology; D002522:Chlorocebus aethiops; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D059871:HLA-A24 Antigen / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D016547:Kinesins / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
C512197:CDH3 protein, human; D015820:Cadherins; D018984:Epitopes, T-Lymphocyte; D059871:HLA-A24 Antigen; C410089:KIF20A protein, human; D010455:Peptides; D007371:Interferon-gamma; D016547:Kinesins