Effective flow cytometric phenotyping of cells using minimal amounts of antibody.
Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproducibly accurate results for the detection of up to seven parameters for activation induced-proliferation, cell cycle analysis, and phenotyping of cell-surface and intracellular antigens.
Department of Oncology, University of Wisconsin School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, USA.
Deepak
D
Sharma
Mark R
MR
Eichelberg
Jill D
JD
Haag
Amanda L
AL
Meilahn
Matthew J
MJ
Muelbl
Kathy
K
Schell
Bart M G
BM
Smits
Michael N
MN
Gould
D000818:Animals; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000191:economics / Q000379:methods*; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D008321:Mammary Glands, Animal / Q000166:cytology; D051379:Mice; D051381:Rats; D018401:Sample Size; D013194:Staining and Labeling / Q000191:economics / Q000379:methods
D015703:Antigens, CD