Sulfur dioxide restores calcium homeostasis disturbance in rat with isoproterenol-induced myocardial injury.
sulfur dioxide (SO₂) could relieve isoproterenol (ISO)-induced myocardial injury, while the mechanism is unclear. This study aims to explore whether the protective effect of SO₂ on ISO-induced myocardial injury was mediated by the restoration of calcium homeostasis disturbance in cardiomyocyte.



Rats were randomly divided into four groups: ISO group, ISO+SO₂ group, control group and SO₂ group. Content of Ca²⁺ in H9c2 cells was assayed using confocal microscope, and cardiac function parameters were measured by echocardiography. Plasma biochemical values and myocardial ultra-structure changes were measured. Meanwhile, the activity, protein and gene levels of sarcoplasmic reticulum Ca²⁺ ATPase (SERCA), and protein and phosphorylation of phospholamban (PLN) were detected. We found SO₂ derivatives could restore the decreased cardiac function, the abnormal lactate dehydrogenase, creatine kinase, alpha-hydroxybutyrate dehydrogenase, potassium, calcium, blood urea nitrogen and the damaged myocardial ultra-structure in rats, and regulate the increased Ca²⁺ content in H9c2 induced by ISO. In addition, compared with ISO group, the decreased activities, protein and mRNA level of SERCA, as well as the decreased protein phosphorylation level of PLN in myocardial tissues were increased in ISO+SO₂ group.



SO₂ derivatives might relieve calcium overload in association with the upregulating expression of SERCA and p-PLN/PLN by myocardial tissues in rats with ISO-induced myocardial injury.
Department of Pediatrics, Peking University First Hospital, Beijing, China.
Shanshan
S
Chen
Junbao
J
Du
Yinfang
Y
Liang
Rongyuan
R
Zhang
Chaoshu
C
Tang
Hongfang
H
Jin
D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D002316:Cardiotonic Agents / Q000633:toxicity; D004195:Disease Models, Animal; D006331:Heart Diseases / Q000378:metabolism; D006335:Heart Injuries / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology*; D007545:Isoproterenol / Q000633:toxicity; D008297:Male; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D013458:Sulfur Dioxide / Q000494:pharmacology*
D002316:Cardiotonic Agents; D013458:Sulfur Dioxide; D007545:Isoproterenol; D002118:Calcium