Neuropeptide analysis with liquid chromatography-capillary electrophoresis-mass spectrometric imaging.
Herein we report the first attempt of coupling multidimensional separations to matrix-assisted laser desorption/ionization (MALDI) mass spectrometric imaging detection. Complex neuropeptide mixtures extracted from crustaceans were first fractionated by reversed-phase liquid chromatography (RPLC), and then subjected to a capillary electrophoresis-mass spectrometric imaging platform. With a specific focus on orcokinin family neuropeptides, we demonstrated that these trace-level analytes from complex neural tissue samples can be fully separated from chemical noise and interfering components and visualized as mass spectrometric imaging signals. A total of 19 putative orcokinins were detected, with highly efficient separations within the family being achieved for the first time. The results indicate that two-dimensional separation coupling to mass spectrometric imaging can serve as a novel and powerful tool in proteomics and peptidomics studies.
School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53705, USA.
Zichuan
Z
Zhang
Chenxi
C
Jia
Lingjun
L
Li
D000595:Amino Acid Sequence; D000818:Animals; D003386:Brachyura / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis* / Q000302:isolation & purification; D010449:Peptide Mapping; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D009479:Neuropeptides