Rapid determination of thiamine, riboflavin, niacinamide, pantothenic acid, pyridoxine, folic acid and ascorbic acid in Vitamins with Minerals Tablets by high-performance liquid chromatography with diode array detector.
A simple, isocratic, and stability-indicating high-performance liquid chromatography (HPLC) method has been developed for the rapid determination of thiamine (VB(1)), niacinamide (VB(3)), pyridoxine (VB(6)), ascorbic acid (VC), pantothenic acid (VB(5)), riboflavin (VB(2)) and folic acid (VB(9)) in Vitamins with Minerals Tablets (VMT). An Alltima C(18) column (250 mm × 4.6 mm i.d., 5 μm) was used for the separation at ambient temperature, with 50mM ammonium dihydrogen phosphate (adjusting with phosphoric acid to pH 3.0) and acetonitrile as the mobile phase at the flow rate of 0.5 ml min(-1). VB(1), VB(3), VB(6), VC and VB(5) were extracted with a solution containing 0.05% phosphoric acid (v/v) and 0.3% sodium thiosulfate (w/v), and were then simultaneously analyzed by using the mobile phase of phosphate buffer-acetonitrile (95:5, v/v), while VB(2) and VB(9) were extracted with a solution containing 0.5% ammonium hydroxide solution (v/v), and were then simultaneously analyzed by using the mobile phase of phosphate buffer-acetonitrile (85:15, v/v). The detection wavelengths were 275 nm for VB(1), VB(3), VB(6), VC, 210 nm for VB(5), and 282 nm for VB(2) and VB(9). The method showed good system suitability, sensitivity, linearity, specificity, precision, stability and accuracy. All the seven water-soluble vitamins were well separated from other ingredients and degradation products. Method comparison indicated good concordance between the developed method and the USP method. The developed method was reliable and convenient for the rapid determination of VB(1), VB(3), VB(6), VC, VB(5), VB(2) and VB(9) in VMT.
Department of Pharmaceutical Science, Beijing Hospital of the Ministry of Health, No. 1 Dahua Road, Dongcheng District, Beijing 100730, China. j790101@163.com
Pengfei
P
Jin
Lufeng
L
Xia
Zheng
Z
Li
Ning
N
Che
Ding
D
Zou
Xin
X
Hu
D000097:Acetonitriles / Q000737:chemistry; D064753:Ammonium Hydroxide; D001205:Ascorbic Acid / Q000032:analysis*; D002021:Buffers; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004338:Drug Combinations; D004355:Drug Stability; D005492:Folic Acid / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000737:chemistry; D057230:Limit of Detection; D009536:Niacinamide / Q000032:analysis*; D010205:Pantothenic Acid / Q000032:analysis*; D010710:Phosphates / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry; D011736:Pyridoxine / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012256:Riboflavin / Q000032:analysis*; D012680:Sensitivity and Specificity; D012995:Solubility; D013607:Tablets; D013696:Temperature; D013831:Thiamine / Q000032:analysis*; D013885:Thiosulfates / Q000737:chemistry; D013997:Time Factors; D014815:Vitamins / Q000032:analysis*
D000097:Acetonitriles; D002021:Buffers; D004338:Drug Combinations; D006878:Hydroxides; D010710:Phosphates; D010756:Phosphoric Acids; D013607:Tablets; D013885:Thiosulfates; D014815:Vitamins; C024788:ammonium phosphate; D010205:Pantothenic Acid; D009536:Niacinamide; D064753:Ammonium Hydroxide; D005492:Folic Acid; C030242:phosphoric acid; C017717:sodium thiosulfate; D011736:Pyridoxine; D001205:Ascorbic Acid; D012256:Riboflavin; D013831:Thiamine; C032159:acetonitrile