Utility of multiple sampling in reducing variation of salivary interleukin-8 and interleukin-1β mRNA levels in healthy adults.
BACKGROUND
Salivary mRNA biomarkers can exhibit significant variation over time. The purpose of this study was to investigate the pattern and magnitude of variation of salivary mRNA biomarkers and to explore the effect of performing replicate assays and multiple sampling on the dispersion of salivary biomarkers.


METHODS
Salivary interleukin-8 (IL-8) and interleukin-1β (IL-1β) mRNAs, developed as biomarkers for oral cancer detection, were selected and measured from 15 healthy subjects at 4 different time points on the same day.


RESULTS
Salivary IL-8 and IL-1β mRNA levels fluctuated randomly over time without predictable rhythm and showed higher within-subject biological variation than analytical variation. The dispersion of salivary IL-8 and IL-1β mRNAs was more effectively reduced by taking multiple saliva sample measurements than by performing replicative assays.


CONCLUSIONS
Taking multiple saliva samples can effectively reduce variation of salivary IL-8 and IL-1β mRNA levels in healthy adults.
Department of Otolaryngology - Head and Neck Surgery, Catholic University of Daegu School of Medicine, 3056-6 Daemyung 4-Dong, Nam-gu, Daegu, South Korea.
Jeong Kyu
JK
Kim
Hui
H
Zhou
Vishad
V
Nabili
Marilene B
MB
Wang
Elliot
E
Abemayor
David T W
DT
Wong
D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry*; D055815:Young Adult
D015415:Biomarkers; D053583:Interleukin-1beta; D016209:Interleukin-8; D012333:RNA, Messenger