Supplementation of xanthophylls increased antioxidant capacity and decreased lipid peroxidation in hens and chicks.
The present study investigated the effects of xanthophyll supplementation on production performance, antioxidant capacity (measured by glutathione peroxidase, superoxide dismutase (SOD), catalase, total antioxidant capacity (T-AOC), and reduced glutathione:oxidised glutathione ratio (GSH:GSSG)) and lipid peroxidation (measured by malondialdehyde (MDA)) in breeding hens and chicks. In Expt 1, 432 hens were fed diets supplemented with 0 (control group), 20 or 40 mg xanthophyll/kg diet. Blood samples were taken at 7, 14, 21, 28 and 35 d of the trial. Liver and jejunal mucosa were sampled at 35 d. Both xanthophyll groups improved serum SOD at 21 and 28 d, serum T-AOC at 21 d and liver T-AOC, and serum GSH:GSSG at 21, 28 and 35 d and liver GSH:GSSG. Xanthophylls also decreased serum MDA at 21 d in hens. Expt 2 was a 2 × 2 factorial design. Male chicks hatched from 0 or 40 mg in ovo xanthophyll/kg diet of hens were fed a diet containing either 0 or 40 mg xanthophyll/kg diet. Liver samples were collected at 0, 7, 14 and 21 d after hatching. Blood samples were also collected at 21 d. In ovo-deposited xanthophylls increased antioxidant capacity and decreased MDA in the liver mainly within 1 week after hatching. Maternal effects gradually vanished during 1-2 weeks after hatching. Dietary xanthophylls increased antioxidant capacity and decreased MDA in the liver and serum mainly from 2 weeks onwards. Data suggested that xanthophyll supplementation enhanced antioxidant capacity and reduced lipid peroxidation in different tissues of hens and chicks.
College of Animal Science, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Yu-Yun
YY
Gao
Qing-Mei
QM
Xie
Jing-Yun
JY
Ma
Xiang-Bin
XB
Zhang
Ji-Mei
JM
Zhu
Ding-Ming
DM
Shu
Bao-Li
BL
Sun
Ling
L
Jin
Ying-Zuo
YZ
Bi
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002374:Catalase / Q000097:blood; D002645:Chickens / Q000097:blood*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D005260:Female; D005978:Glutathione / Q000032:analysis / Q000097:blood; D019803:Glutathione Disulfide / Q000032:analysis / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D007413:Intestinal Mucosa / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000737:chemistry; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000097:blood; D013482:Superoxide Dismutase / Q000032:analysis / Q000097:blood; D024341:Xanthophylls / Q000008:administration & dosage*
D000975:Antioxidants; D024341:Xanthophylls; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D019803:Glutathione Disulfide