cDNA-SRAP and its application in differential gene expression analysis: a case study in Erianthus arundinaceum.
Erianthus arundinaceum is a wild relative species of sugarcane. The aim of this research was to demonstrate the feasibility of cDNA-SRAP for differential gene expression and to explore the molecular mechanism of drought resistance in E. arundinaceum. cDNA-SRAP technique, for the first time, was applied in the analysis of differential gene expression in E. arundinaceum under drought stress. In total, eight differentially expressed genes with length of 185-427 bp were successfully isolated (GenBank Accession numbers: EU071770, EU071772, EU071774, EU071776, EU071777, EU071779, EU071780, and EU071781). Based on their homologies with genes in GenBank, these genes were assumed to encode ribonuclease III, vacuolar protein, ethylene insensitive protein, aerobactin biosynthesis protein, photosystem II protein, glucose transporter, leucine-rich repeat protein, and ammonia monooxygenase. Real-time PCR analysis on the expression profiling of gene (EU071774) encoding ethylene-insensitive protein and gene (EU071781) encoding ammonia monooxygenase revealed that the expression of these two genes was upregulated both by PEG and ABA treatments, suggesting that they may involve in the drought resistance of E. arundinaceum. This study constitutes the first report of genes activated in E. arundinaceum by drought stress and opens up the application of cDNA-SRAP in differential gene expression analysis in E. arundinaceum under certain stress conditions.
Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. queyouxiong@hotmail.com
Youxiong
Y
Que
Liping
L
Xu
Jianwei
J
Lin
Jun
J
Luo
Jingsheng
J
Xu
Jin
J
Zheng
Rukai
R
Chen
D000222:Adaptation, Physiological / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis* / Q000737:chemistry; D018744:DNA, Plant / Q000032:analysis* / Q000737:chemistry; D055864:Droughts; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D008954:Models, Biological; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D031786:Saccharum / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics
D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D010088:Oxidoreductases; C045582:ammonia monooxygenase