[Significance of comprehensive gene expression analysis for evaluation of biological effects of manufactured nanomaterials].
The industrial applications of manufactured nanomaterials (MNs) are expected to be extended to next-generation devices. On the other hand, concern over the effects of MNs on human health has risen owing to advances in the development of nanotechnology. Indeed, little is known about the mechanism of action of MNs. The New Energy and Industrial Technology Development Organization of Japan (NEDO) launched a new research project entitled "Evaluating risks associated with manufactured nanomaterials (P10024)" in 2006. The project demonstrated no adverse effects of MN inhalation exposure on the rat lungs, as determined by histopathological examination and bronchoalveolar lavage (BAL) fluid analysis. In parallel with this research, we have performed comparative gene expression analysis using DNA microarrays in rat lungs after inhalation exposure (4 weeks, 6 hours a day, 5 days a week) to single-wall nanotubes (SWCNTs), multiwall nanotubes (MWCNTs), C<inf>60</inf> fullerene and ultrafine nickel oxide particles (Uf-NiO) as reference materials for the purpose of gaining insights into the molecular events following the exposure. In this review, we introduce an outline of the project, and discuss about the significance of comparative gene expression analysis for evaluation of the biological effects of MNs.
National Institute of Advanced Industrial Science and Technology, Japan. ka-fujita@aist.go.jp
Katsuhide
K
Fujita
Makiko
M
Fukuda
Hitoshi
H
Iwahashi
D000280:Administration, Inhalation; D000818:Animals; D037741:Fullerenes; D015870:Gene Expression; D008168:Lung / Q000187:drug effects; D049329:Nanostructures / Q000008:administration & dosage / Q000633:toxicity*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats
D037741:Fullerenes; C069837:fullerene C60