Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups.
Using data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherichia coli isolates rapidly to major phylogenetic groups. This assay accommodates sequence variations detected within target sequences, thereby increasing sensitivity and reliability. It was validated using 185 isolates of known sequence types and showed improved congruence with multilocus sequence typing data.
Microbiology Services-Colindale, Health Protection Agency, London, United Kingdom. michel.doumith@hpa.org.uk
M
M
Doumith
M J
MJ
Day
R
R
Hope
J
J
Wain
N
N
Woodford
D001431:Bacteriological Techniques / Q000379:methods*; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity