Metatranscriptomic analysis of small RNAs present in soybean deep sequencing libraries.
A large number of small RNAs unrelated to the soybean genome were identified after deep sequencing of soybean small RNA libraries. A metatranscriptomic analysis was carried out to identify the origin of these sequences. Comparative analyses of small interference RNAs (siRNAs) present in samples collected in open areas corresponding to soybean field plantations and samples from soybean cultivated in greenhouses under a controlled environment were made. Different pathogenic, symbiotic and free-living organisms were identified from samples of both growth systems. They included viruses, bacteria and different groups of fungi. This approach can be useful not only to identify potentially unknown pathogens and pests, but also to understand the relations that soybean plants establish with microorganisms that may affect, directly or indirectly, plant health and crop production.
Centro de Biotecnologia e PPGBCM, Laboratório de Genomas e Populações de Plantas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Lorrayne Gomes
LG
Molina
Guilherme Cordenonsi
GC
da Fonseca
Guilherme Loss
GL
de Morais
Luiz Felipe Valter
LF
de Oliveira
Joseane Biso
JB
de Carvalho
Franceli Rodrigues
FR
Kulcheski
Rogerio
R
Margis