Persorption of IgG-Fc-coated particulates from intestinal lumen into portal blood via villous columnar epithelial cells in rat small intestine.
Previously, the specific antibody-mediated persorption of antigenic molecules and particulates from the small-intestinal lumen into the peripheral blood was clarified in rats, but the intermediation of the receptor for the specific antibodies was not. In this study, the existence of receptor for the specific antibody was experimentally examined in the rat small intestine. Glutaraldehyde-fixed sheep erythrocytes (SEs) coated by Fc-fragments of IgG (IgG-Fc), (Fab')(2)-fragments of IgG (IgG-Fab) or bovine serum albumin (BSA), were injected into 3 jejunal loops each 2 cm in length in non-orally pre-immunized rats, respectively. Thirty minutes after the injection, IgG-Fc-coated SEs were significantly more engulfed by villous columnar epithelial cells than Fab- or BSA-coated SEs. The most frequent absorption sites were the intestinal villous apices. The IgG-Fc-coated SEs were adhered to the striated borders and were engulfed by villous columnar epithelial cells. IgG-Fc-coated SEs passing through the epithelial cells were also detected in the subepithelial blood capillaries just beneath the villous epithelium, but not in the connective tissue and the lymph vessels. These findings suggest that the absorption of luminal antigenic particulates is probably mediated by the Fc-receptor, and that the absorbed antigenic particulates are directly transferred to the hepatic portal blood by passing through the endothelium of the subepithelial blood capillaries.
Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe, Japan.
Midori
M
Yuji
Masato
M
Fujimoto
Wang-Mei
WM
Qi
Ei-Ichrou
EI
Takahara
Youhei
Y
Mantani
Kankanam Gamage Sanath
KG
Udayanga
Takashi
T
Takeuchi
Katsuhiko
K
Warita
Toshifumi
T
Yokoyama
Nobuhiko
N
Hoshi
Hiroshi
H
Kitagawa
D000818:Animals; D004847:Epithelial Cells / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism*; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D007583:Jejunum / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D007141:Immunoglobulin Fc Fragments