[Quickly investigating the absorption ingredients of Gentianae Radix et Rhizoma by SEMAC].
OBJECTIVE
To establish a method for quickly investigating the absorption ingredients which could be used as the index of quality control of Gentianae Radix et Rhizoma.


METHODS
The absorption ingredients of Gentianae Radix et Rhizoma were investigated by using the model of in vitro everted intestinal sac (VEIS). The intestinal sac liquors of jejunum and ileum were collected at 6 intervals (15, 30, 45, 60, 90, 120 min) and gentiopicroside, loganin acid, swertiamarin and sweroside were detected by HPLC as the representative marker. The accumulative absorption quantity of gentiopicroside, loganin acid, swertiamarin and sweroside were calculated, respectively.


RESULTS
Six components could be detected in intestinal sac. In different concentrations of Gentianae Radix et Rhizoma, gentiopicroside and swertiamarin in various intestinal sections were the linear absorption (R2 > 0.9), conformed to zero order absorption rate. In jejunum the constant of absorption rate (Ka) of gentiopicroside and swertiamarin increased with the raised dosage of Gentianae Radix et Rhizoma (P < 0.05), which indicated a passive absorption manner, and the value of Ka of high and middle dosage of those in ileum were higher than that of low dosage, and the difference of Ka between high and middle dosage were not significant, which indicated a positive absorption manner. The Ka of high and middle dosage of sweroside in ileum and jejunum were higher than that of low dosage (P < 0.05), and the difference of Ka between high and middle dosage were not significant, which indicated a positive absorption manner. The Ka of loganin acid in jejunum and ileum increased along with the raised dosage of Gentianae Radix et Rhizoma (P < 0.05), which indicated a passive absorption manner.


CONCLUSIONS
SEMAC could be used as a tool to investigate the absorption ingredients of Gentianae Radix et Rhizoma. Drug in intestine sac was selective, and the absorption part of intestine was also different.
Shaanxi University of Chinese Medicine, Xi'an 712046, China.
Bei
B
Song
Jingjing
J
Zhu
Weihao
W
Wang
Guang
G
Hu
Xiaomei
X
Song
Zhimin
Z
Wang
D000042:Absorption; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D030012:Gentiana / Q000737:chemistry*; D007082:Ileum / Q000378:metabolism; D057888:Iridoid Glucosides / Q000493:pharmacokinetics; D007583:Jejunum / Q000378:metabolism; D008297:Male; D011753:Pyrones / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D057888:Iridoid Glucosides; D011753:Pyrones; C012997:gentiopicroside; C013270:swertiamarin