Effect of inositol hexaphosphate-loaded red blood cells (RBCs) on the rheology of sickle RBCs.
BACKGROUND
The recent in vitro demonstration that inositol hexaphosphate-loaded red blood cells (IHP-RBCs) may reduce the risks of sickling of sickle RBCs (SS RBCs) exposed to hypoxia make these modified RBCs potentially useful in transfused sickle cell anemia (SCA) patients.


METHODS
Hemorheologic properties of IHP-RBCs, normal RBCs (AA RBCs), SS RBCs, SS RBCs plus AA RBCs, and SS RBCs plus IHP-RBCs were compared under normoxia and/or after hypoxic challenges.


RESULTS
Although IHP-RBCs have reduced deformability compared with SS RBCs or AA RBCs, IHP-RBCs exhibited lower aggregability than AA RBCs and SS RBCs and, when mixed with SS RBCs, the aggregation level was below the one of SS RBCs alone or SS RBCs plus AA RBCs. Blood viscosity of SS RBC plus IHP-RBC suspension was lower than the viscosity of SS RBCs alone and greater than viscosity of SS RBCs plus AA RBCs. The hypoxic challenge was detrimental for deformability and viscosity of SS RBCs alone or SS plus AA RBC suspension but not for SS plus IHP-RBC suspension.


CONCLUSIONS
Our results support the fact that IHP-RBCs could be useful in SCA by decreasing RBC aggregation and blunting the adverse effects of hypoxia on RBC deformability and blood viscosity.
UMR Inserm U665, Pointe-à-Pitre, Guadeloupe, France.
Yann
Y
Lamarre
Vanessa
V
Bourgeaux
Aurélien
A
Pichon
Max R
MR
Hardeman
Yannick
Y
Campion
Marise
M
Hardeman-Zijp
Cyril
C
Martin
Jean-Paul
JP
Richalet
Françoise
F
Bernaudin
Françoise
F
Driss
Yann
Y
Godfrin
Philippe
P
Connes
D000755:Anemia, Sickle Cell / Q000097:blood* / Q000473:pathology; D001809:Blood Viscosity / Q000187:drug effects; D004903:Erythrocyte Aggregation / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D004913:Erythrocytes, Abnormal / Q000187:drug effects* / Q000473:pathology; D018056:Hemorheology / Q000187:drug effects*; D006801:Humans; D009996:Osmotic Fragility / Q000187:drug effects; D010833:Phytic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D033081:Shear Strength / Q000187:drug effects; D013314:Stress, Mechanical
D010833:Phytic Acid