[Metabonomic study on siwu tang in radiation-induced blood deficient mice].
OBJECTIVE
To study the radiation-induced blood deficiency and the combination of prescription and syndrome of Siwu Tang using ultra performance liquid chromatography-quadrupoles-time of flight mass spectrometer (UPLC-ESI-Q-TOF-MS), in order to discover the changes in metabolic profiles of blood deficient mice induced by radiation, and clarify the relationship between blood deficient syndrome and the mechanism of Siwu Tang.


METHODS
Thirty six C57 mice were randomly divided into three groups: the control group and the model model groups and the Siwu Tang group. The model was established by general irradiation with 3.5 Gy60 Coy ray. The animals were sacrificed on the 7th day after radiation and their blood, spleens and thymus were collected and detected using UPLC-ESI-Q-TOF/MS. MarkerLynx XS software was adopted to identify chromatographic peaks in the total ion chromatogram. Data was processed by making principal component analysis and analyzed by orthogonal partial least squares method among these groups, in order to rapidly identify marks through pattern recognition and analysis.


RESULTS
Compared with the control group, lysophosphatide, glucosiduronic acid, monoacylglycerol, erythronic acid, ceramide, aspartate phosphate ester, glyceryl phosphatide were obviously changed in the sera of the model group. Monoacylglycerol, ceramide, lysophosphatide, hydroxybutyric acid, palmitinic acid, 3-hydroxystearic acid, diethylarginine, neuraminic acid, phosphatidylserine were found in metabolites of their spleens. Methyladenosine, sitosterol, carnitine, phosphatidylinositol, phosphatidylethanolamine, diglyceride, dimethylarginine, ceramide, hydroxybutyric acid, cholesterol were found in thymus of the model group.


CONCLUSIONS
Analysis on physiological functions of these biological markers show that radiation could induce the disorder of the metabolism of lipoid and carbohydrate and affect the synthesis of some amino acids, and Siwu Tang can reverse these effects.
Beijing Institute of Radiation Medicine, Military Medical Science Academy of PLA, Beiing 100850, China. 13681121635@139.com
Zengchun
Z
Ma
Chao
C
Huo
Sisi
S
Zhou
Qiande
Q
Liang
Yuguang
Y
Wang
Hongling
H
Tan
Chengrong
C
Xiao
Yue
Y
Gao
D000818:Animals; D002241:Carbohydrates / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D008055:Lipids / Q000097:blood; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL
D002241:Carbohydrates; D004365:Drugs, Chinese Herbal; D008055:Lipids