[Effect comparison of Wright-Giemsa stain under different conditions and its apply in cell micronucleus test].
OBJECTIVE
To apply the Wright-Giemsa stain in micronucleus test and to explore the stain outcomes of Wright-Giemsa dye of various proportions and staining times.


METHODS
Use Wright-Giemsa dye, Wright dye (staining time 3 min) and Giemsa dye (staining time 5 min) to stain HepG2 and then observe the staining effect. The Wright-Giemsa dye was applied under 5 different proportions (3:1-1:3) and different staining times (1, 3, 5, 10, 15 min).


RESULTS
After stained for 3-5 min with the proportion ratio of 3:1 of Wright-Giemsa dye, the HepG2 cells showed much better staining outcomes compared with the single stain of either Wright or Giemsa.


CONCLUSIONS
Wright-Giemsa stain can be used in cell micronucleus test to obtain good staining outcomes.
Shajing Institute of Inspection, Shenzhen, Guangdong Province, China.
Chun-hua
CH
Liao
Huan-feng
HF
Bian
Fang
F
Xiao
D001399:Azure Stains; D004396:Coloring Agents; D056945:Hep G2 Cells; D006801:Humans; D015162:Micronucleus Tests; D013194:Staining and Labeling / Q000379:methods*
D001399:Azure Stains; D004396:Coloring Agents