[Cloning beta-tubulin from Fusarium fujikuroi to analyze its relationship with carbendazim resistance].
OBJECTIVE
We studied the resistance of Fusarium fujikuroi against carbendazim, to confirm whether the resistance was related to the beta-tubulin gene.


METHODS
The isolated strains were identified based on TEF-1alpha gene sequence and morphological characteristics. The whole nucleotide sequence of beta-tubulin gene was analyzed from different sensitive strains by the primers designed according to the nucleotide sequence of beta-tubulin gene from the sequenced strain 7600 of F. verticillioides. Real-time quantitative RT-PCR (qRT-PCR) was applied to analyze the expression pattern of beta-tubulin gene from different sensitive strains treated with or without carbendazim.


RESULTS
The full-length nucleotide sequence of beta-tubulin gene (Accession Number: JQ026022) was cloned from different sensitive strains of F. fujikoroi, which spanned 1671 bp with 4 introns, encoding 447 amino acids. This gene showed 100% nucleotide sequence homology between sensitive and resistant strains. The expression of the beta-tubulin gene in 2 sensitive strains was significantly higher than 3 resistant strains when these strains were cultured on carbendazim-free media (p = 0.05). Carbendazim at the concentration of their EC50 caused the expression of the gene from the five strains increasing significantly (p = 0.05) in comparison with that of strains treated without carbendazim. However, there was no significant difference between strains when treated with carbendazim.


CONCLUSIONS
The resistance of F. fujikuroi against carbendazim was irrelative to beta-tubulin gene.
College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Pestcides of Jiangsu Province, Nanjing 210095, China. maxiaoweiying@126.com
Xiaowei
X
Ma
Chunjie
C
Xing
Jinfeng
J
Yu
Yong
Y
Wang
Zihao
Z
Chen
Jianxin
J
Wang
Mingguo
M
Zhou
Changjun
C
Chen
D001562:Benzimidazoles / Q000494:pharmacology*; D002219:Carbamates / Q000494:pharmacology*; D003001:Cloning, Molecular; D005670:Fusarium / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism
D001562:Benzimidazoles; D002219:Carbamates; D014404:Tubulin; C006698:carbendazim