Identification  of ANAPC13 gene polymorphisms associated with body measurement traits in Bos  taurus.
Body  measurement traits, influenced by genes and environmental factors, play  numerous important roles in the value assessment of productivity and economy.  There has been some indication that ANAPC13 influences adult height. We used  PCR-SSCP and DNA sequencing technology to identify polymorphisms in the  ANAPC13 gene. A polymorphism in intron 1 (A > G at base 17) was identified  and an additional polymorphic site (C > T at base 42) was also uncovered,  which accompanied the previous polymorphism in more than 98% of the subjects.  The two novel polymorphisms in exon 1 were assayed and potential associations  with body measurement traits were evaluated in 404 individuals. Three genotypes  were detected in the study group, named AACC, AGCT and GGTT. Significant  differences were observed between genotypes AACC and AGCT for body length,  withers height, hip height, hip width, heart girth, pin bone width. However, no  associations were found among any genotypes and chest depth. We conclude that  polymorphisms and mutations in non-coding regions of the ANAPC13 gene  significantly affect body measurement traits.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
B J
BJ
Jiang
X L
XL
Zhan
C Z
CZ
Fu
H B
HB
Wang
G
G
Cheng
L S
LS
Zan
D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D001483:Base Sequence; D001837:Body Weights and Measures; D002417:Cattle / Q000033:anatomy & histology* / Q000235:genetics*; D016009:Chi-Square Distribution; D002681:China; D005091:Exons / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D056426:Genetic Loci / Q000235:genetics; D005838:Genotype; D016018:Least-Squares Analysis; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA; D043743:Ubiquitin-Protein Ligase Complexes / Q000235:genetics*
D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome