Molecular characterization of stress resistance-related chitinase genes of Brassica rapa.
Brassica is an important vegetable group worldwide that is impacted by biotic and abiotic stresses. Molecular biology techniques offer the most efficient approach to address these concerns. Inducible plant defense responses include the production of pathogenesis-related (PR) proteins, and chitinases are very important PR proteins. We collected 30 chitinase like genes, three from our full-length cDNA library of Brassica rapa cv. Osome and 27 from Brassica databases. Sequence analysis and comparison study confirmed that they were all class I-V and VII chitinase genes. These genes also showed a high degree of homology with other biotic stress resistance-related plant chitinases. An organ-specific expression of these genes was observed and among these, seven genes showed significant responses after infection with Fusarium oxysporum f.sp. conglutinans in cabbage and sixteen genes showed responsive expression after abiotic stress treatments in Chinese cabbage. BrCLP1, 8, 10, 17 and 18 responded commonly after biotic and abiotic stress treatments indicating their higher potentials. Taken together, the results presented herein suggest that these chitinase genes may be useful resources in the development of stress resistant Brassica.
Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-742, Republic of Korea.
Nasar Uddin
NU
Ahmed
Jong-In
JI
Park
Hee-Jeong
HJ
Jung
Kwon-Kyoo
KK
Kang
Yoonkang
Y
Hur
Yong-Pyo
YP
Lim
Ill-Sup
IS
Nou
D000222:Adaptation, Physiological / Q000235:genetics*; D001483:Base Sequence; D001937:Brassica / Q000235:genetics / Q000378:metabolism; D029741:Brassica rapa / Q000235:genetics* / Q000378:metabolism; D002688:Chitinases / Q000235:genetics*; D018076:DNA, Complementary; D060467:Disease Resistance / Q000235:genetics; D005670:Fusarium; D015870:Gene Expression; D017343:Genes, Plant; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017421:Sequence Analysis; D017385:Sequence Homology; D013312:Stress, Physiological / Q000235:genetics
D018076:DNA, Complementary; D010940:Plant Proteins; D002688:Chitinases