A majority of infectious Newcastle disease virus particles contain a single genome, while a minority contain multiple genomes.
Paramyxoviruses produce pleiomorphic particles containing variable amounts of genetic material that correlate with virion diameter by electron microscopy. However, the infectious nature of these particles is unknown, and functional genomes are indistinguishable from defective RNA. A quantitative approach to paramyxovirus packaging revealed a majority of infectious Newcastle disease viruses contain one functional genome. Virions encapsidating two or three genomes (approximately 25% of total) were also observed by utilizing three different recombinant viruses expressing unique fluorescent reporters.
Department of Microbiology, Mount Sinai School of Medicine, New York, New York, USA.
Peter H
PH
Goff
Qinshan
Q
Gao
Peter
P
Palese
D000818:Animals; D002642:Chick Embryo; D004262:DNA Restriction Enzymes / Q000378:metabolism; D016679:Genome, Viral; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007700:Kinetics; D009521:Newcastle Disease / Q000378:metabolism / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics* / Q000378:metabolism*; D034622:RNA Interference; D012367:RNA, Viral / Q000378:metabolism; D014771:Virion / Q000235:genetics; D019065:Virus Assembly / Q000235:genetics
D012367:RNA, Viral; D049452:Green Fluorescent Proteins; D004262:DNA Restriction Enzymes