Establishment of a monitoring system to detect inhibition of mRNA processing.
A number of proteins complete mRNA processing in the nucleus, thus, inhibitor of mRNA processing is worth finding to analyze the mechanism of mRNA maturation in detail. Here, we established a monitoring system for mRNA processing using a test compound, spliceostatin A (SSA), which inhibits mRNA splicing. This system should serve to facilitate the discovery of novel compounds from natural resources that inhibit mRNA processing.
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
Ken-ichi
K
Fujita
Masumi
M
Okamura
Sachiko
S
Nishimoto
Tomoya
T
Kurihara
Keiko
K
Momma
Yusaku
Y
Miyamae
Taiho
T
Kambe
Masaya
M
Nagao
Hiroshi
H
Narita
Seiji
S
Masuda
D001681:Biological Assay; D002467:Cell Nucleus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D049410:Luciferases, Renilla; D011714:Pyrans / Q000494:pharmacology*; D012322:RNA Precursors / Q000037:antagonists & inhibitors / Q000235:genetics; D012326:RNA Splicing / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000235:genetics; D013141:Spiro Compounds / Q000494:pharmacology*; D017381:Spliceosomes / Q000187:drug effects / Q000235:genetics
D011714:Pyrans; D012322:RNA Precursors; D012333:RNA, Messenger; D013141:Spiro Compounds; C553684:spliceostatin A; D049410:Luciferases, Renilla