Isolation and characterisation of aerobic endospore forming Bacilli from sugarcane rhizosphere for the selection of strains with agriculture potentialities.
Eighteen aerobic endospore forming strains were isolated from sugarcane rhizosphere in N-free medium. A phenotypic description and analysis of the 5' end hypervariable region sequences of 16S rRNA revealed a high diversity of Bacillus and related genera. Isolates were identified, and four genera were obtained: seven strains belonged to Bacillus (Bacillaceae family), four belonged to Paenibacillus, six belonged to Brevibacillus and one strain was identified as Cohnella (Paenibacillaceae family). Four Brevibacillus strains showed in vitro inhibitory activity against plant pathogens fungi Curvularia and Fusarium. Seventy-four percent of the isolated bacteria grew on pectin as the only carbon source, showing polygalacturonase activity. Pectate lyase activity was detected for the first time in a Brevibacillus genus strain. All isolates showed endoglucanase activity. Calcium phosphate solubilisation was positive in 83.3% of the isolates, with higher values than those reported for Bacillus inorganic phosphate solubilising strains. High ethylene plant hormone secretion in the culture medium was detected in 22% of the bacteria. This is the first report of ethylene secretion in Paenibacillaceae isolates. Indole-3-acetic acid production was found in a Brevibacillus genus isolate. It was reported for the first time the presence of Cohnella genus strain on sugarcane rhizosphere bearing plant growth promoting traits. The sugarcane isolate Brevibacillus B65 was identified as a plant growth inoculant because it showed wider spectra of plant stimulation capabilities, including an antifungal effect, extracellular hydrolases secretion, inorganic phosphate solubilisation and plant hormone liberation. In this work, sugarcane was shown to be a suitable niche for finding aerobic endospore forming 'Bacilli' with agriculture biotechnological purposes.
Centro de Estudios de Biotecnología Industrial, Facultad de Ciencias Naturales, Universidad de Oriente, Av. Patricio Lumumba S/N, 90500, Santiago de Cuba, Cuba. torbera@cebi.uo.edu.cu
Teresa
T
de Los Milagros Orberá Ratón
Ricardo
R
Yano
Odalys
O
Rodríguez Gámez
Eny Iochevet Segal
EI
Floh
Manuel
M
de Jesús Serrat Díaz
Heloíza Ramos
HR
Barbosa
D000383:Agriculture / Q000379:methods; D000898:Antibiosis; D056449:Bacillales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000254:growth & development; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D031786:Saccharum / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S