Characterization of MHC-II antigen presentation by B cells and monocytes from older individuals.
In this study we examine the effects of aging on antigen presentation of B cells and monocytes. We compared the antigen presentation function of peripheral blood B cells from young and old subjects using a system that specifically measures the B cell receptor (BCR)-mediated MHC-II antigen presentation. Monocytes were studied as well. Overall the mean magnitude of antigen presentation of soluble antigen and peptide was not different in older and younger subjects for both B cells and monocytes. Older subjects, however, showed increased heterogeneity of BCR-mediated antigen presentation by their B cells. The magnitude and variability of peptide presentation, which do not require uptake and processing, were the same between groups. Presentation by monocytes had similar variability between the older and younger subjects. These data suggest that poor B cell antigen processing, which results in diminished presentation in some older individuals may contribute to poor vaccine responses.
Division of Infectious Disease, Case Western Reserve University School of Medicine. 10900 Euclid Ave, BRB 1001, Cleveland, OH 44106-4984, USA. hlc36@case.edu
H L
HL
Clark
R
R
Banks
L
L
Jones
T R
TR
Hornick
P A
PA
Higgins
C J
CJ
Burant
D H
DH
Canaday
D000368:Aged; D000369:Aged, 80 and over; D017951:Antigen Presentation / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000276:immunology / Q000378:metabolism
D000949:Histocompatibility Antigens Class II; D011947:Receptors, Antigen, B-Cell