Immortalized mouse fetal liver stromal cells support growth and maintenance             of human embryonic stem cells.
Human embryonic stem cells (hESCs) are usually maintained in an undifferentiated             state by co-culture with feeder cells. The feeder cells are important for the             growth of hESCs. A novel spontaneously immortalizated mouse fetal liver stromal             cell line, named KM3, was isolated from a 13.5‑day mouse fetal liver. In this             study, we examined whether KM3 cells could be used as feeders to support the growth             of hESCs. hESCs cultured on KM3 cells showed a similar proliferation rate and             characteristics to mouse embryonic fibroblasts (MEFs) after prolonged culture,             including morphology, unlimited and undifferentiated proliferative ability, maintenance             of normal karyotypes, formation of embryoid bodies in vitro and typically immature             teratomas in vivo. Our results indicate that the immortalized KM3 cell line has             the potential to support the growth and maintenance of hESCs. The cell line may             be used for the large-scale expansion of hESCs in a low-cost and less labor-intensive             manner.
School of Medical Science and Laboratory Medicine, Jiangsu University, and Department of Clinical Laboratory, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Jiangsu 212013, PR China. hu@ujs.edu.cn
Jiabo
J
Hu
Sanqiang
S
Hu
Quanhui
Q
Ma
Xiaohui
X
Wang
Zhongwei
Z
Zhou
Wei
W
Zhang
Xiaochun
X
Sun
Wei
W
Zhu
Hui
H
Qian
Wenrong
W
Xu
D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D058732:Embryoid Bodies; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D059785:Karyotype; D008099:Liver / Q000166:cytology / Q000196:embryology*; D051379:Mice; D016513:Mice, SCID; D016685:Mitomycin / Q000494:pharmacology; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000166:cytology; D055748:SOXB1 Transcription Factors / Q000235:genetics; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects; D013724:Teratoma / Q000473:pathology
D015415:Biomarkers; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D016685:Mitomycin