Effect of short peptides on expression of signaling molecules in organotypic pineal cell culture.
We demonstrated the influence of short peptides on the expression of signaling molecules in organotypic culture of the pineal gland from 3-month-old rats. Peptides Ala-Glu-Asp-Gly and Lys-Glu-Asp stimulate the expression of proliferative protein Ki-67 in pineal gland culture. These peptides as well as Glu-Asp-Arg and Lys-Glu do not affect the expression of apoptosis marker AIF. The synthesis of transcription factor CGRP by pinealocytes was stimulated only by Ala-Glu-Asp-Gly. Thus, peptide Ala-Glu-Asp-Gly tissue-specifically stimulates proliferative and secretory activities of pinealocytes, which can be used for recovery of pineal gland functions at the molecular level.
St. Petersburg Institute of Bioregulation and Gerontology, NorthWestern Branch of the Russian Academy of Medical Sciences, Russia.
V Kh
VKh
Khavinson
N S
NS
Linkova
N I
NI
Chalisova
A V
AV
Dudkov
E A
EA
Koncevaya
D000818:Animals; D051033:Apoptosis Inducing Factor / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D004151:Dipeptides / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D010870:Pineal Gland / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D046509:Tissue Culture Techniques
C494831:Aifm1 protein, rat; D051033:Apoptosis Inducing Factor; D015415:Biomarkers; D004151:Dipeptides; D036341:Intercellular Signaling Peptides and Proteins; D019394:Ki-67 Antigen; D009842:Oligopeptides; C533570:lysyl-glutamyl-aspartic acid; C570174:pinealon; C415909:lysylglutamic acid; D015740:Calcitonin Gene-Related Peptide; C421253:alanyl-glutamyl-aspartyl-glycine