Isolation and characterization of  microsatellite DNA loci from the peanut worm, Sipunculus nudus.
Sipunculus  nudus, the peanut worm, is the best known species in its  genus. This unsegmented subtidal marine worm is consumed in some parts of Asia  and is also used as fish bait. We found 20 microsatellite DNA markers for S.  nudus. The number of alleles per polymorphic locus ranged from two to seven  in a sample of 39 individuals. Observed and expected heterozygosities per  polymorphic locus varied from 0.103 to 1.000 and from 0.307 to 0.771,  respectively. Five loci showed significant departure from Hardy-Weinberg  equilibrium after sequential Bonferroni's correction. No significant linkage  disequilibrium between pairs of loci was found. These microsatellite markers  will provide useful tools for investigating genetic population structure,  population history and conservation management of S. nudus.
Fisheries College, Guangdong Ocean University, Zhanjiang, China.
Q H
QH
Wang
Y S
YS
Guo
Z D
ZD
Wang
X D
XD
Du
Y W
YW
Deng
D000818:Animals; D005819:Genetic Markers / Q000235:genetics; D015810:Linkage Disequilibrium / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D009348:Nematoda / Q000235:genetics*
D005819:Genetic Markers