Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant.
A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.
Department of Marine Biotechnology, Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, Maryland, USA. Schreier@umbc.edu
Harold J
HJ
Schreier
Wipawee
W
Dejtisakdi
Jose Ortega
JO
Escalante
Marina
M
Brailo
D003470:Culture Media / Q000737:chemistry; D004251:DNA Transposable Elements; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism; D016254:Mutagenesis, Insertional / Q000379:methods*; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000172:deficiency* / Q000378:metabolism; D061328:Planctomycetales / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D003470:Culture Media; D004251:DNA Transposable Elements; D019764:Phosphoenolpyruvate Carboxykinase (ATP); D005947:Glucose