Chemical composition and bioactivities of a water-soluble polysaccharide from the endodermis of shaddock.
The chemical composition of shaddock (Citrus paradisi) mainly consisted of polyphenols, proteins and polysaccharides. However, polysaccharides from shaddock materials have received much less consideration than polyphenols. Herein, a water-soluble neutral polysaccharide from the endodermis of shaddock was isolated and showed good bioactivities. Crude polysaccharides from the endodermis of shaddock (EPS) was extracted with hot water and separated on a DEAE Sepharose FF gel filtration column to obtain NEPS. The IR and UV spectra of NEPS showed that NEPS was mainly composed of polysaccharide and there are no proteins existing in NEPS. The DPPH radical scavenging and reducing power of NEPS are much lower than those of crude EPS; however, Citrus flavonoids significantly improved the DPPH radical scavenging potential and reducing power of NEPS. The crude EPS (5mg/mL) showed a similar inhibitory effect (77.92±5.03%) with NEPS (5 mg/mL) (74.63±4.71%) on α-amylase.
Institute of Advanced Materials & Chembiosensing Technology, Yulin Normal University, Yulin, Guangxi, PR China. guocongliu@yahoo.com
Guocong
G
Liu
Shuwen
S
Xu
Longsheng
L
Chen
D001713:Biphenyl Compounds / Q000737:chemistry; D032083:Citrus paradisi / Q000737:chemistry* / Q000166:cytology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D012995:Solubility; D014867:Water / Q000737:chemistry*
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007004:Hypoglycemic Agents; D010851:Picrates; D011134:Polysaccharides; D014867:Water; C004931:1,1-diphenyl-2-picrylhydrazyl