Effect of dirhamnolipid on the removal of phenol catalyzed by laccase in aqueous solution.
In this study, the effects of three surfactants, i.e. the anionic biosurfactant dirhamnolipid (diRL), the cationic surfactant hexadecyltrimethyl ammonium bromide (CTAB), and the anionic surfactant sodium dodecyl sulfate (SDS), on the removal of phenol catalyzed by laccase were studied first. CTAB and SDS were detrimental, while diRL improved phenol removal and was selected for detailed research. The biosurfactant increased the activity of laccase and the removal of phenol with the increase of diRL concentrations from 10.6 to 318 μM. DiRL at 318 μM improved the removal when the initial concentrations of phenol were from 50 to 400 mg/l. In particular, the removal of phenol with 318 μM diRL was 4.3-6.4 folds that of the controls within 24 h when the initial concentration of phenol was 400 mg/l. The presence of diRL at 318 μM also caused the complete removal (above 98%) of phenol at concentrations from 50 to 400 mg/l after 24 h. The enhancement of phenol removal was over a wide range of pH and temperatures, and the highest removal efficiency was obtained at pH 6.0 and 50°C. The results suggest that diRL had potential application in the enhancement of phenols removal catalyzed by laccase in water treatment or remediation.
College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China. lzf18182002@163.com
Zhi-Feng
ZF
Liu
Guang-Ming
GM
Zeng
Hua
H
Zhong
Xing-Zhong
XZ
Yuan
Hai-Yan
HY
Fu
Mei-Fang
MF
Zhou
Xiao-Ling
XL
Ma
Hui
H
Li
Jian-Bing
JB
Li
D001673:Biodegradation, Environmental; D002384:Catalysis; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D006017:Glycolipids / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000378:metabolism*; D019800:Phenol / Q000302:isolation & purification / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012996:Solutions; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature; D055454:Trametes / Q000201:enzymology; D014867:Water; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*
D002593:Cetrimonium Compounds; D006017:Glycolipids; D012996:Solutions; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; C051310:rhamnolipid hemolysin, Pseudomonas aeruginosa; D014867:Water; D019800:Phenol; D012967:Sodium Dodecyl Sulfate; D042845:Laccase; D000077286:Cetrimonium