DNA and RNA pattern of staining during oogenesis in zebrafish (Danio rerio): a confocal microscopy study.
Oogenesis involves a sequence of cellular divisions and developmental changes leading to the formation of oocytes, whose role in development is to transfer genomic information to the next generation. During this process, the gene expression pattern changes considerably concomitant with genome remodeling, while genomic information is maintained. The development of the gonad in zebrafish is unique in that it goes through an initial ovarian phase and subsequently into either ovarian or testicular phases. How the germ cells choose to commit to an oogenic fate and enter meiosis or alternatively not to enter meiosis and commit to a spermatogenetic fate remains a key question in development. Lack of suitable markers has hampered the understanding of the principles controlling sex differentiation in zebrafish. The current study was aimed at finding substantive cytochemical markers to identify specific oocyte stages primarily focusing on the DNA and RNA component of cells, using fluorescent dyes: acridine orange and propidium iodide. The pattern of synthesis and appearance of nucleoli was stage specific and may be used to identify stages of oogenesis. A distinguishing and possibly diagnostic feature of the staining pattern observed was the low level of chromatin staining compared to other cellular structures. This may be related to the more diffuse state of chromatin that occurs prior to thickening of chromosomes from the pachytene stage onwards. Although the fluorescent dyes may be useful in determining the localization of nucleic acids in tissue sections, it was not possible to quantify the relative contribution of the DNA and RNA components of specific stages of oocyte growth.
Department of Zoology, Walter Sisulu University, P/B X1 Mthatha, 5117, South Africa. ageokuthe@gmail.com
Grace Emily
GE
Okuthe
D000165:Acridine Orange / Q000494:pharmacology; D000818:Animals; D019070:Cell Lineage; D004247:DNA / Q000378:metabolism*; D005260:Female; D005456:Fluorescent Dyes / Q000494:pharmacology; D005854:Germ Cells / Q000166:cytology; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D009865:Oocytes / Q000166:cytology; D009866:Oogenesis; D009867:Oogonia; D010053:Ovary / Q000502:physiology; D011419:Propidium / Q000494:pharmacology; D012313:RNA / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism; D013194:Staining and Labeling; D013737:Testis / Q000502:physiology; D015027:Zebrafish
D005456:Fluorescent Dyes; D011419:Propidium; D012313:RNA; D004247:DNA; D012260:Ribonucleases; D000165:Acridine Orange