Involvement of NMDA receptors in the antidepressant-like action of adenosine.
METHODS
In this work, the contribution of NMDA receptors to the antidepressant-like effect of adenosine in the forced swimming test (FST) was investigated.


RESULTS
The pretreatment of mice with NMDA or D-serine was able to prevent the anti-immobility effect of either adenosine or MK-801 in the FST. In addition, the administration of a sub-effective dose of adenosine produced a synergistic effect with sub-effective doses of MK-801, ketamine and zinc chloride. Moreover, the immobility time of the mice treated with active doses of adenosine or N(6)-cyclohexyladenosine (CHA) plus MK-801 was not significantly different from that obtained with adenosine, CHA and MK-801 alone; by contrast, the combination between active doses of adenosine and CHA plus an active dose of the tricyclic antidepressant imipramine produced a greater effect in the FST than the administration of either drug alone.


CONCLUSIONS
Together, the results suggest that the effect of adenosine in the FST is likely dependent on the inhibition of NMDA receptors mediated by the activation of adenosine A(1) receptors.
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, Brazil. manu.kaster@gmail.com
Manuella P
MP
Kaster
Daniele G
DG
Machado
Adair R S
AR
Santos
Ana Lúcia S
AL
Rodrigues
D000241:Adenosine / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology; D002712:Chlorides / Q000008:administration & dosage / Q000494:pharmacology; D003863:Depression / Q000188:drug therapy*; D016291:Dizocilpine Maleate / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D007099:Imipramine / Q000008:administration & dosage / Q000494:pharmacology; D007103:Immobilization; D007649:Ketamine / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D051379:Mice; D016202:N-Methylaspartate / Q000494:pharmacology; D043682:Receptor, Adenosine A1 / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D012694:Serine / Q000494:pharmacology; D013550:Swimming; D013997:Time Factors; D017967:Zinc Compounds / Q000008:administration & dosage / Q000494:pharmacology
D000928:Antidepressive Agents; D000929:Antidepressive Agents, Tricyclic; D002712:Chlorides; D043682:Receptor, Adenosine A1; D016194:Receptors, N-Methyl-D-Aspartate; D017967:Zinc Compounds; D012694:Serine; D016202:N-Methylaspartate; D007649:Ketamine; D016291:Dizocilpine Maleate; C016837:zinc chloride; D000241:Adenosine; D007099:Imipramine