An excitation ratiometric Zn2+ sensor with mitochondria-targetability for monitoring of mitochondrial Zn2+ release upon different stimulations.
A mitochondria-targeted fluorescent sensor (Mito-ST), constructed by integrating a sulfamoylbenzoxadiazole fluorophore with a phosphonium group, displays the specific Zn(2+)-induced hypsochromic shifts of both excitation (69 nm) and emission (35 nm) maxima. Its ratiometric Zn(2+) imaging ability via dual excitation mode has been applied in MCF-7 cells to clarify the different behaviours of mitochondrial Zn(2+) release stimulated by H(2)O(2) and SNOC.
State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, PR China.
Zhipeng
Z
Liu
Changli
C
Zhang
Yuncong
Y
Chen
Weijiang
W
He
Zijian
Z
Guo
D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D061986:MCF-7 Cells; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D061848:Optical Imaging; D015032:Zinc / Q000737:chemistry* / Q000493:pharmacokinetics
D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide; D015032:Zinc