The effect of titanium with electrochemical anodization on the response of the adherent osteoblast-like cell.
OBJECTIVE
The osseointegration of titanium dental implants is essential for successful therapy, and this is strongly affected by the surface chemistry and roughness. In this study, using electrochemical anodization after sand blasting, and acid etching of Ti surface (SLA), SLA specimens with the high wettability of the thick TiO2 layer (SLAffinity) surface was developed to superimpose the nanoscale topographies on the microscale roughness of SLA surface without greatly altering the surface features.


METHODS
The surface characteristics of SLAffinity specimens were investigated using scanning electron microscopy, atomic force microscopy, and x-ray photoelectron spectroscopy. The viability and proliferation of osteoblast-like cells (MG63 cells), which were seeded on SLAffinity specimens, were analyzed.


RESULTS
Such electrochemically anodized specimens were predominantly composed of bioactive TiO2. The cell culturing tests revealed that the microscale roughness in combination with the nanoscale structures and bioactive properties improved osteoblast viability and differentiation on the SLAffinity surface.


CONCLUSIONS
The favorable biological response of SLAffinity surfaces to MG63 osteoblast-like cells suggested that electrochemical anodization after SLA treatments is a potential procedure for better osseointegration in vivo.
School of Dentistry, Taipei Medical University, Taipei, Taiwan.
Yun-Ho
YH
Lin
Pei-Wen
PW
Peng
Keng-Liang
KL
Ou
D000134:Acid Etching, Dental / Q000379:methods; D000469:Alkaline Phosphatase / Q000032:analysis; D000537:Aluminum Oxide / Q000737:chemistry; D017554:Carbon Compounds, Inorganic / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D004396:Coloring Agents; D045662:Dental Etching / Q000379:methods; D003764:Dental Materials / Q000737:chemistry*; D055664:Electrochemical Techniques; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry; D010006:Osteoblasts / Q000502:physiology*; D056951:Photoelectron Spectroscopy; D017655:Silicon Compounds / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry; D013499:Surface Properties; D013778:Tetrazolium Salts; D013844:Thiazoles; D014025:Titanium / Q000737:chemistry*; D017155:Wettability
D017554:Carbon Compounds, Inorganic; D004396:Coloring Agents; D003764:Dental Materials; D017655:Silicon Compounds; D013464:Sulfuric Acids; D013778:Tetrazolium Salts; D013844:Thiazoles; C009495:titanium dioxide; D014025:Titanium; D000469:Alkaline Phosphatase; C022616:thiazolyl blue; D000537:Aluminum Oxide; D006851:Hydrochloric Acid; C022088:silicon carbide