Dual-modal fluorescent/magnetic bioprobes based on small sized upconversion nanoparticles of amine-functionalized BaGdF5:Yb/Er.
A new type of BaGdF(5):Yb/Er nanoprobe for dual-modal fluorescent and magnetic resonance imaging (MRI) is demonstrated. Water soluble and amine-functionalized BaGdF(5):Yb/Er nanoparticles (NPs) with average size of about 10 nm were synthesized by a facile one-pot hydrothermal method. The in vitro up-converted emission of BaGdF(5):Yb/Er NPs is observed in HeLa cells with near-infrared excitation at 980 nm and served as a fluorescent label. In addition, the cytotoxicity assay in HeLa cells shows low cell toxicity of the amine-functionalized BaGdF(5):Yb/Er NPs. Moreover, these BaGdF(5) NPs exhibit excellent intrinsic paramagnetic properties and enhanced T(1)-weighted MRI images with increased concentrations of BaGdF(5) NPs. Therefore, these results suggest that the amine-functionalized BaGdF(5) NPs with an optimized size and low cell toxicity are promising dual-modal bioprobes for optical bioimaging and MRI.
Department of Applied Physics and Materials Research Center, The Hong Kong Polytechnic University, Hong Kong.
Songjun
S
Zeng
Ming-Kiu
MK
Tsang
Chi-Fai
CF
Chan
Ka-Leung
KL
Wong
Bin
B
Fei
Jianhua
J
Hao
D000588:Amines / Q000737:chemistry*; D015374:Biosensing Techniques; D005063:Europium / Q000737:chemistry* / Q000633:toxicity; D005456:Fluorescent Dyes / Q000737:chemistry*; D005682:Gadolinium / Q000737:chemistry* / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D015018:Ytterbium / Q000737:chemistry* / Q000633:toxicity
D000588:Amines; D005456:Fluorescent Dyes; D005063:Europium; D005682:Gadolinium; D015018:Ytterbium