siRNA interference with a proliferation-inducing ligand gene in the Sgr-7901 gastric carcinoma cell line.
OBJECTIVE
The present study aimed to investigate the influence of siRNA interference with a proliferation- inducing ligand (APRIL) gene on gastric carcinoma sgr-7901 cell apoptosis. Correlations between APRIL silencing and tyrosine kinase (trka) expression were also explored.


METHODS
Two APRIL-silencing siRNA vectors were constructed, and transfected into human gastric carcinoma sgr-7901 cells, expression before and after transfection being detected using RT-PCR and western blot analyses. The expression of 15 trka genes was detected using RT- PCR and apoptotic rates of sgr-7901 were assessed by flow cytometry.


RESULTS
The expression levels of receptor trka genes were significantly decreased, and the apoptotic rate of sgr-7901 was significantly increased after transfection (P < 0.05).


CONCLUSIONS
APRIL gene silencing can increase the apoptotic rate of gastric carcinoma cells, and inhibit the expression of receptor trka genes. There is a correlation between the signaling pathways of APRIL and trka.
Department of General Surgery, First Affiliated Hospital, Nanjing, China.
Shao-Zhong
SZ
Ni
Hong-Yong
HY
Cao
Zheng
Z
Chen
Yi
Y
Zhu
Ze-Kuan
ZK
Xu
D017209:Apoptosis; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids; D034622:RNA Interference; D034741:RNA, Small Interfering; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000235:genetics* / Q000378:metabolism
D034741:RNA, Small Interfering; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13; D020794:Receptor Protein-Tyrosine Kinases