Using genipin-crosslinked acellular porcine corneal stroma for cosmetic corneal lens implants.
Acellular porcine corneal stroma (APCS) has been proven to maintain the matrix microenvironment and is therefore an ideal biomaterial for the repair and reconstruction of corneal stroma. This study aims to develop a method to prepare cosmetic corneal lens implants for leukoma using genipin-crosslinked APCS (Gc-APCS). The Gc-APCS was prepared from APCS immersed in 1.0% genipin aqueous solution (pH 5.5) for 4 h at 37 °C, followed by lyophilization at -10 °C. The color of the Gc-APCS gradually deepened to dark-blue. The degree of crosslinking was 45.7 ± 4.6%, measured by the decrease of basic and hydroxy amino acids. The porous structure and ultrastructure of collagenous lamellae were maintained, and the porosity and BET SSA were 72.7 ± 4.6% and 23.01 ± 3.45 m(2)/g, respectively. The Gc-APCS rehydrated to the physiological water content within 5 min and was highly resistant to collagenase digestion. There were no significant differences in the areal modulus and curvature variation between Gc-APCS and nature porcine cornea. The dark-blue pigments were stable to pH, light and implantation in vivo. Gc-APCS extracts had no inhibitory effects on the proliferation of keratocytes. Corneal neovascularization, graft degradation and corneal rejection were not observed within 6 months.
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, PR China.
Zhao
Z
Liu
Qiang
Q
Zhou
Jixiang
J
Zhu
Jianhui
J
Xiao
Pengxia
P
Wan
Chenjing
C
Zhou
Zheqian
Z
Huang
Na
N
Qiang
Wei
W
Zhang
Zheng
Z
Wu
Daping
D
Quan
Zhichong
Z
Wang
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017364:Collagenases / Q000378:metabolism; D060527:Corneal Keratocytes / Q000166:cytology / Q000187:drug effects; D003319:Corneal Stroma / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D003358:Cosmetics / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005260:Female; D005612:Freeze Drying; D020007:Implants, Experimental; D039823:Iridoids / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000187:drug effects*; D008297:Male; D008422:Materials Testing; D016062:Porosity; D011817:Rabbits; D034421:Sus scrofa; D054457:Tissue Scaffolds / Q000737:chemistry
D000596:Amino Acids; D003358:Cosmetics; D003432:Cross-Linking Reagents; D039823:Iridoids; C007834:genipin; D017364:Collagenases