Enzymatic synthesis of puerarin glucosides using Leuconostoc dextransucrase.
Puerarin (P), an isoflavone derived from kudzu roots, has strong biological activities, but its bioavailability is often limited by its low water solubility. To increase its solubility, P was glucosylated by three dextransucrases from Leuconostoc or Streptococcus species. Leuconostoc lactis EG001 dextransucrase exhibited the highest productivity of puerarin glucosides (P-Gs) among the three tested enzymes, and it primarily produced two P-Gs with a 53% yield. Their structures were identified as alpha-D-glucosyl-(1-->6)-P (P-G) by using LC-MS or (1)H- or (13)C-NMR spectroscopies and alpha-D-isomaltosyl-(1-->6)-P (P-IG2) by using specific enzymatic hydrolysis, and their solubilities were 15- and 202-fold higher than that of P, respectively. P-G and P-IG2 are easily applicable in the food and pharmaceutical industries as alternative functional materials.
Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Korea.
Jin-A
JA
Ko
Young Bae
YB
Ryu
Tae-Soon
TS
Park
Hyung Jae
HJ
Jeong
Jang-Hoon
JH
Kim
Su-Jin
SJ
Park
Joong-Su
JS
Kim
Doman
D
Kim
Young-Min
YM
Kim
Woo Song
WS
Lee
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005960:Glucosides / Q000096:biosynthesis* / Q000737:chemistry; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006031:Glycosylation; D007529:Isoflavones / Q000096:biosynthesis* / Q000737:chemistry; D007934:Leuconostoc / Q000201:enzymology* / Q000235:genetics; D012995:Solubility
D001426:Bacterial Proteins; D005960:Glucosides; D007529:Isoflavones; D005964:Glucosyltransferases; C020025:dextransucrase; C033607:puerarin