Neisseria oralis sp. nov., isolated from healthy gingival plaque and clinical samples.
A polyphasic analysis was undertaken of seven independent isolates of gram-negative cocci collected from pathological clinical samples from New York, Louisiana, Florida and Illinois and healthy subgingival plaque from a patient in Virginia, USA. The 16S rRNA gene sequence similarity among these isolates was 99.7-100 %, and the closest species with a validly published name was Neisseria lactamica (96.9 % similarity to the type strain). DNA-DNA hybridization confirmed that these isolates are of the same species and are distinct from their nearest phylogenetic neighbour, N. lactamica. Phylogenetic analysis of 16S and 23S rRNA gene sequences indicated that the novel species belongs in the genus Neisseria. The predominant cellular fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C18 : 1ω7c. The cellular fatty acid profile, together with other phenotypic characters, further supports the inclusion of the novel species in the genus Neisseria. The name Neisseria oralis sp. nov. (type strain 6332(T)  = DSM 25276(T)  = LMG 26725(T)) is proposed.
Department of Biomedical Sciences, State University of New York, PO Box 22002, Albany, NY 12201, USA.|Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
William J
WJ
Wolfgang
Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
Teresa V
TV
Passaretti
Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
Reashma
R
Jose
Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
Jocelyn
J
Cole
Laboratory of Microbiology (LM-UGent), Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium.|Department of Applied Engineering Sciences, Laboratory of Biochemistry and Brewing, University College Ghent, Schoonmeersstraat 52, 9000 Ghent, Belgium.
An
A
Coorevits
Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
Andrea N
AN
Carpenter
Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
Sherly
S
Jose
Laboratory of Microbiology (LM-UGent), Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium.|Department of Applied Engineering Sciences, Laboratory of Biochemistry and Brewing, University College Ghent, Schoonmeersstraat 52, 9000 Ghent, Belgium.
Anita
A
Van Landschoot
Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USA.|Department of Molecular Genetics, The Forsyth Institute, Cambridge, MA 02142, USA.
Jacques
J
Izard
Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
Donna J
DJ
Kohlerschmidt
Laboratory of Microbiology (LM-UGent), Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium.
Peter
P
Vandamme
Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USA.|Department of Molecular Genetics, The Forsyth Institute, Cambridge, MA 02142, USA.
Floyd E
FE
Dewhirst
Department of Pathology, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84132, USA.|Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT, USA.
Mark A
MA
Fisher
Department of Biomedical Sciences, State University of New York, PO Box 22002, Albany, NY 12201, USA.|Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
Kimberlee A
KA
Musser
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D005881:Gingiva / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D009343:Neisseria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014481:United States
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S