Pseudomonas syringae Catalases Are Collectively Required for Plant Pathogenesis.
The bacterial pathogen Pseudomonas syringae pv. tomato DC3000 must detoxify plant-produced hydrogen peroxide (H(2)O(2)) in order to survive in its host plant. Candidate enzymes for this detoxification include the monofunctional catalases KatB and KatE and the bifunctional catalase-peroxidase KatG of DC3000. This study shows that KatG is the major housekeeping catalase of DC3000 and provides protection against menadione-generated endogenous H(2)O(2). In contrast, KatB rapidly and substantially accumulates in response to exogenous H(2)O(2). Furthermore, KatB and KatG have nonredundant roles in detoxifying exogenous H(2)O(2) and are required for full virulence of DC3000 in Arabidopsis thaliana. Therefore, the nonredundant ability of KatB and KatG to detoxify plant-produced H(2)O(2) is essential for the bacteria to survive in plants. Indeed, a DC3000 catalase triple mutant is severely compromised in its ability to grow in planta, and its growth can be partially rescued by the expression of katB, katE, or katG. Interestingly, our data demonstrate that although KatB and KatG are the major catalases involved in the virulence of DC3000, KatE can also provide some protection in planta. Thus, our results indicate that these catalases are virulence factors for DC3000 and are collectively required for pathogenesis.
Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska, USA.
Ming
M
Guo
Anna
A
Block
Crystal D
CD
Bryan
Donald F
DF
Becker
James R
JR
Alfano
D017360:Arabidopsis / Q000382:microbiology; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D044224:Pseudomonas syringae / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D037521:Virulence Factors / Q000378:metabolism*
D037521:Virulence Factors; D006861:Hydrogen Peroxide; D002374:Catalase