Attenuation of cellular antioxidant defense mechanisms in kidney of rats intoxicated with carbofuran.
Carbofuran, an anticholinestrase carbamate, is commonly used as an insecticide. Its toxic effect on kidney is less established. The present study was designed to investigate the effect of carbofuran on kidneys and to understand the mechanism involved in its nephrotoxicity. Male Wistar rats were divided into two groups of eight animals each; control animals received sunflower oil (vehicle) and carbofuran exposed animals were treated with carbofuran (1 mg/kg body weight) orally for 28 days. At the end of the treatment, significant increase was observed in urea and creatinine levels in serum along with the inhibition of acetylcholinesterase, suggesting nephrotoxicity. The antioxidant defense system of animals treated with carbofuran was altered in terms of increased lipid peroxidation, reduced glutathione, and total thiols and decreased activity of antioxidant enzymes (superoxide dismutase and catalase). The results indicate that carbofuran is nephrotoxic and increased oxidative stress appears to be involved in its nephrotoxic effects.
Department of Biochemistry, Panjab University, Chandigarh, India.
Bhupindervir
B
Kaur
Alka
A
Khera
Rajat
R
Sandhir
D000110:Acetylcholinesterase / Q000097:blood / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002235:Carbofuran / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000503:physiopathology; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014508:Urea / Q000097:blood
D000975:Antioxidants; D007306:Insecticides; D013438:Sulfhydryl Compounds; D014508:Urea; D003404:Creatinine; D002374:Catalase; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase; D002235:Carbofuran