Cloning and characterization of porcine interferon-δ-related genes identified by genomic database screening.
Interferon-δ (IFN-δ) belongs to type I IFN. It was first identified by Lefevre in 1993. The porcine interferon-δ (PoIFN-δ)-related sequences were reported in 2009. The IFN-δ-related sequences have not been studied as closely as IFN-α and IFN-β. In this study, we identified 11 functional genes and 7 pseudogenes of the PoIFN-δ-related subtypes from the porcine genome database. Three of these genes were cloned, expressed, and functionally studied. PoIFN-δ-related subtypes can bind to the type I IFN receptors, activate the Janus kinase-signal transduce and activator of transcription (JAK-STAT) signaling pathway, induce IFN stimulate genes, and other cytokines. The antiviral activity of PoIFN-δ-related subtypes varies a lot in different subtypes. Among all the PoIFN-δ subtypes detected, PoIFN-δ8 has the strongest antiviral and immunomodulatory activities.
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, PR China.
Xin
X
Zhao
Gong
G
Cheng
Ye
Y
Jiao
Weiyao
W
Yan
Mingqiu
M
Liu
Zhaoxin
Z
Zheng
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D006224:Cricetinae; D016207:Cytokines / Q000235:genetics; D015870:Gene Expression; D056426:Genetic Loci; D016678:Genome; D050835:Interferon Regulatory Factors / Q000235:genetics* / Q000378:metabolism; D007370:Interferon Type I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D053612:Janus Kinases / Q000378:metabolism; D062105:Molecular Docking Simulation; D010802:Phylogeny; D011485:Protein Binding; D053633:Receptor, Interferon alpha-beta / Q000737:chemistry / Q000378:metabolism; D050791:STAT Transcription Factors / Q000378:metabolism; D015398:Signal Transduction; D013552:Swine / Q000235:genetics / Q000378:metabolism
D000998:Antiviral Agents; D016207:Cytokines; D050835:Interferon Regulatory Factors; D007370:Interferon Type I; D050791:STAT Transcription Factors; C037655:interferon, delta; D053633:Receptor, Interferon alpha-beta; D053612:Janus Kinases