Alternative reprogramming of M1/M2 phenotype of mouse peritoneal macrophages in vitro with interferon-γ and interleukin-4.
An important role in the development of the immune response is played by macrophages that acquire either anti-inflammatory M1 or anti-inflammatory M2 phenotype depending on their microenvironment. The possibility of targeted reprogramming of the initial M2 macrophage phenotype towards M1 phenotype and vice versa using macrophage reprogramming factors IFN-γ and IL-4, respectively, was demonstrated. We showed that macrophages of genetically different mouse strains did not practically differ by their reprogramming capacity. Our findings suggest that macrophage programming not only participates in the triggering of the immune response, but also can ensure plasticity of functional activity during the developing response.
Moscow State University of Medicine and Dentistry, Ministry of Health Care and Social Development of the Russian Federation, Russian Academy of Medical Sciences, Moscow, Russia.
S V
SV
Lyamina
S V
SV
Kruglov
T Yu
TY
Vedenikin
O A
OA
Borodovitsyna
I A
IA
Suvorova
Sh L
ShL
Shimshelashvili
I Yu
IY
Malyshev
D000818:Animals; D019070:Cell Lineage / Q000187:drug effects* / Q000276:immunology; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology*; D015847:Interleukin-4 / Q000276:immunology / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism
D008070:Lipopolysaccharides; D015847:Interleukin-4; D009569:Nitric Oxide; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse