Semi-automated direct elution of dried blood spots for the quantitative determination of guanfacine in human blood.
BACKGROUND
Direct analysis of dried blood spot (DBS) samples was investigated using a prototype semi-automated robotic device that allows the direct elution of sample spots from a DBS paper card to an online SPE cartridge. The eluted SPE samples were analyzed with high-performance LC-MS/MS.


RESULTS
A LLOQ of 0.01 ng/ml was achieved with a linear calibration range from 0.01 to 25 ng/ml. Optimal performance data were obtained from spotting the internal standard solution on the card before blood spotting. Internal standard addition from the system injector loop produced intra-assay inaccuracy of -9.0-7.3% and precision of 1.3-8.2%, and inter-assay inaccuracy of -3.5-3.9% and precision of 4.4-8.7%.


CONCLUSIONS
Results demonstrated the feasibility of a semi-automated online rapid direct elution method that avoids manual extraction for DBS sample analysis using the online DBS-SPE system coupled to LC-MS/MS.
Advion Bioanalytical Labs, Ithaca, NY, USA.
Yuanyuan
Y
Li
Jack
J
Henion
Richard
R
Abbott
Phillip
P
Wang
D058647:Adrenergic alpha-2 Receptor Agonists / Q000097:blood*; D000818:Animals; D057205:Automation, Laboratory / Q000379:methods; D002138:Calibration; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D059788:Dried Blood Spot Testing / Q000295:instrumentation* / Q000379:methods*; D016316:Guanfacine / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D012015:Reference Standards; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
D058647:Adrenergic alpha-2 Receptor Agonists; D016316:Guanfacine