Free radical scavenging activity of 4-(3,4-dihydroxybenzoyloxymethyl)phenyl-O-β-D-glucopyranoside from Origanum vulgare and its protection against oxidative damage.
4-(3,4-Dihydroxybenzoyloxymethyl)phenyl-O-β-d-glucopyranoside (DBPG), a polyphenolic glycoside, isolated from  Origanum vulgare  has shown 1,1-diphenyl-2-picrylhydrazyl (DPPH(•))-scavenging capacity in previous work. This study demonstrated that DBPG exhibits antioxidant activity by a series of DPPH(•), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS(•+)), and superoxide anion radical (O(2)(•-)) radical-scavenging assays. The inhibition of lipid peroxidation (LP) by DBPG exceeded that by l-ascorbic acid (AA) in a liposome model system. Adding DBPG to mouse liver and brain tissue inhibited the formation of thiobarbituric acid reactive substances (TBARS) to a greater extent than did trolox. In the oxygen stress test, BNLCL2 and HaCaT cells pretreated with DBPG showed increased activities of glutathione peroxidase (GPx), perhaps as a result of reduction of the production of reactive oxygen species (ROS). These findings proved that DBPG had antioxidant activities and a cytoprotective effect in hepatocytes and keratinocytes, suggesting that DBPG may be a useful food and cosmetic additive.
Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.
Chia-Hua
CH
Liang
Leong-Perng
LP
Chan
Hsiou-Yu
HY
Ding
Edmund Cheung
EC
So
Rong-Jyh
RJ
Lin
Hui-Min
HM
Wang
Ying-Ging
YG
Chen
Tzung-Han
TH
Chou
D000818:Animals; D002460:Cell Line; D016166:Free Radical Scavengers / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D008757:Methylglucosides / Q000032:analysis / Q000494:pharmacology*; D051379:Mice; D031341:Origanum / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*
C529825:4-(3,4-dihydroxybenzoyloxymethyl)phenyl-O-beta-D-glucopyranoside; D016166:Free Radical Scavengers; D008757:Methylglucosides; D010936:Plant Extracts