Antiphotoaging effects of light-emitting diode irradiation on narrow-band ultraviolet B-exposed cultured human skin cells.
BACKGROUND
Antiaging effects of light-emitting diodes (LEDs) have been clinically demonstrated using one or two wavelengths. The mechanism is unclear.


OBJECTIVE
To examine direct and indirect photobiomodulation effects of LEDs on narrow-band ultraviolet B (NB-UVB)-induced photoaging using seven different wavelengths alone or in combination.


METHODS
Four LED wavelengths were chosen based on type I collagen and metalloproteinase (MMP)-1 expression. NB-UVB-irradiated fibroblasts or keratinocytes were irradiated using these four wavelengths. The expression of collagen and MMP-1 in fibroblasts with or without conditioned medium from LED-irradiated keratinocytes and the expression of proinflammatory cytokines in the LED-irradiated keratinocytes were examined.


RESULTS
Irradiation with four wavelengths (630, 660, 830, and 850 nm) significantly increased the number of viable fibroblasts. These four wavelengths also increased type I collagen expression, particularly four combinations (630/830, 660/850, 630/850, and 660/830 nm). The fibroblasts cultured with the keratinocyte conditioned medium, particularly with a combination of 630/850 or 660/830 nm, increased collagen levels. Low tumor necrosis factor alpha (TNF-α) and high transforming growth factor beta 1 (TGF-β1) expression was detected in the LED-irradiated keratinocytes.


CONCLUSIONS
The combination of 630/850- or 660/830-nm irradiation led to better direct and indirect antiphotoaging outcomes than single LED wavelengths in NB-UVB-irradiated cultured normal human skin cells.
Department of Dermatology, Dongguk University Ilsan Hospital, Goyang-si, Gyeonggi-do, South Korea.
Yu Shun
YS
Tian
Nan-Hyung
NH
Kim
Ai-Young
AY
Lee
D000704:Analysis of Variance; D049109:Cell Proliferation / Q000528:radiation effects; D024042:Collagen Type I / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D010789:Phototherapy / Q000379:methods; D015595:Skin Aging; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
D024042:Collagen Type I; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D020781:Matrix Metalloproteinase 1