Uptake of ant-derived nitrogen in the myrmecophytic orchid Caularthron bilamellatum.
OBJECTIVE
Mutualistic ant-plant associations are common in a variety of plant families. Some myrmecophytic plants, such as the epiphytic orchid Caularthron bilamellatum, actively form hollow structures that provide nesting space for ants (myrmecodomatia), despite a substantial loss of water-storage tissue. This study aimed at assessing the ability of the orchid to take up nitrogen from ant-inhabited domatia as possible trade-off for the sacrifice of potential water storage capacity.


METHODS
Nitrogen uptake capabilities and uptake kinetics of (15)N-labelled compounds (NH(4)(+), urea and l -glutamine) were studied in field-grown Caularthron bilamellatum plants in a tropical moist forest in Panama. Plants were either labelled directly, by injecting substrates into the hollow pseudobulbs or indirectly, by labelling of the associated ants in situ.


RESULTS
Caularthron bilamellatum plants were able to take up all tested inorganic and organic nitrogen forms through the inner surface of the pseudobulbs. Uptake of NH(4)(+) and glutamine followed Michaelis-Menten kinetics, but urea uptake was not saturable up to 2 mm. (15)N-labelled compounds were rapidly translocated and incorporated into vegetative and reproductive structures. By labelling ants with (15)N in situ, we were able to prove that ants transfer N to the plants under field conditions.


CONCLUSIONS
Based on (15)N labelling experiments we were able to demonstrate, for the first time, that a myrmecophytic orchid is capable of actively acquiring different forms of nitrogen from its domatia and that nutrient flux from ants to plants does indeed occur under natural conditions. This suggests that beyond anti-herbivore protection host plants benefit from ants by taking up nitrogen derived from ant debris.
Department of Terrestrial Ecosystem Research, University of Vienna, Austria.
Christian
C
Gegenbauer
Veronika E
VE
Mayer
Gerhard
G
Zotz
Andreas
A
Richter
D000818:Animals; D001000:Ants / Q000502:physiology*; D001692:Biological Transport; D005973:Glutamine / Q000378:metabolism; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes / Q000032:analysis; D029595:Orchidaceae / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D010176:Panama; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013559:Symbiosis / Q000502:physiology*; D014329:Tropical Climate; D014508:Urea / Q000378:metabolism
D009587:Nitrogen Isotopes; D000644:Quaternary Ammonium Compounds; D005973:Glutamine; D014508:Urea; D009584:Nitrogen