Neonatally induced mild diabetes in rats and its effect on maternal, placental, and fetal parameters.
The aim of this study was to assess placental changes and reproductive outcomes in neonatally induced mild diabetic dams and fetal development in their offspring. At birth, female rats were assigned either to control or diabetic group (100 mg of streptozotocin/Kg, subcutaneously). At adulthood, the female rats were mated. During pregnancy, the blood glucose levels and glucose and insulin tolerance tests were performed. At term, maternal reproductive outcomes, fetal and placental weight, and placental morphology were analyzed. Diabetic rats had smaller number of living fetuses, implantations and corpora lutea, and increased rate of embryonic loss. Placenta showed morphometric alterations in decidua area. Our results showed that mild diabetes was sufficient to trigger alterations in maternal organism leading to impaired decidua development contributing to failure in embryonic implantation and early embryonic losses. Regardless placental decidua alteration, the labyrinth, which is responsible for the maternal-fetal exchanges, showed no morphometric changes contributing to an appropriate fetal development, which was able to maintain normal fetal weight at term in mild diabetic rats. Thus, this experimental model of diabetes induction at the day of birth was more effective to reproduce the reproductive alterations of diabetic women.
Laboratory of Experimental Research in Gynecology and Obstetrics, Department of Gynecology and Obstetrics, Botucatu Medical School, Universidade Estadual Paulista, Distrito Rubião Júnior 18618000 s/n, Botucatu, SP, Brazil.
Yuri Karen
YK
Sinzato
Gustavo Tadeu
GT
Volpato
Isabela Lovizutto
IL
Iessi
Aline
A
Bueno
Iracema de Mattos Paranhos
Ide M
Calderon
Marilza Vieira Cunha
MV
Rudge
Débora Cristina
DC
Damasceno
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000503:physiopathology*; D016640:Diabetes, Gestational / Q000378:metabolism / Q000503:physiopathology*; D004195:Disease Models, Animal; D005260:Female; D020567:Fetal Weight; D005951:Glucose Tolerance Test; D007328:Insulin / Q000378:metabolism; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D001786:Blood Glucose; D007328:Insulin