FimA, FimF, and FimH are necessary for assembly of type 1 fimbriae on Salmonella enterica serovar Typhimurium.
Salmonella enterica serovar Typhimurium is a Gram-negative member of the family Enterobacteriaceae and is a common cause of bacterial food poisoning in humans. The fimbrial appendages are found on the surface of many enteric bacteria and enable the bacteria to bind to eukaryotic cells. S. Typhimurium type 1 fimbriae are characterized by mannose-sensitive hemagglutination and are assembled via the chaperone/usher pathway. S. Typhimurium type 1 fimbrial proteins are encoded by the fim gene cluster (fimAICDHFZYW), with fimAICDHF expressed as a single transcriptional unit. The structural components of the fimbriae are FimA (major subunit), FimI, FimH (adhesin), and FimF (adaptor). In order to determine which components are required for fimbrial formation in S. Typhimurium, mutations in fimA, fimI, fimH, and fimF were constructed and examined for their ability to produce surface-assembled fimbriae. S. Typhimurium SL1344ΔfimA, -ΔfimH, and -ΔfimF mutants were unable to assemble fimbriae, indicating that these genes are necessary for fimbrial production in S. Typhimurium. However, SL1344ΔfimI was able to assemble fimbriae. In Escherichia coli type 1 and Pap fimbriae, at least two adaptors are expressed in addition to the adhesins. However, E. coli type 1 and Pap fimbriae have been reported to be able to assemble fimbriae in the absence of these proteins. These results suggest differences between the S. Typhimurium type 1 fimbrial system and the E. coli type 1 and Pap fimbrial systems.
Department of Microbiology, University of Iowa College of Medicine, Iowa City, Iowa, USA.
Sarah A
SA
Zeiner
Brett E
BE
Dwyer
Steven
S
Clegg
D036781:Fimbriae Proteins / Q000235:genetics* / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D017353:Gene Deletion; D005798:Genes, Bacterial; D046911:Macromolecular Substances / Q000378:metabolism*; D005810:Multigene Family; D055503:Protein Multimerization; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D046911:Macromolecular Substances; D036781:Fimbriae Proteins