Involvement of STAT3, NF-κB and associated downstream molecules before and after the onset of urethane induced lung tumors in mouse.
Here we have shown the alteration of transcription factors STAT3, NF-κB and downstream associated molecules much before the appearance of lung tumor and their response to antitumor agent, inositol hexaphosphate. Histological examination revealed the pathophysiology of the lung tissues and the onset or progression of tumor from 4 or 9 to 24 weeks in terms of tumor volume and the number. Over expression of NF-κB (p50/Rel A), COX-2, STAT3, pSTAT3 (Tyr 705), IL-6 and cyclin D1 also progressed from the time of no tumor to the time of tumor appearance and was reduced in mice drinking 2%IP6. We suggest that the alterations of STAT3, NF-κB and downstream associated molecules are critical in the development of lung tumors and can be exploited as possible mechanisms after the exposure. Status of these altered genes before the tumor development suggests their possible use as targets for the tumor control in the predisposed conditions.
Environmental Carcinogenesis Division, CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Lucknow 226001, India.
Manuraj
M
Pandey
Environmental Carcinogenesis Division, CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Lucknow 226001, India. Electronic address: krishnag522@yahoo.co.in.
Krishna P
KP
Gupta
D000818:Animals; D002273:Carcinogens; D019938:Cyclin D1 / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D015850:Interleukin-6 / Q000235:genetics; D008175:Lung Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D014520:Urethane
D002273:Carcinogens; D015850:Interleukin-6; D016328:NF-kappa B; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; D019938:Cyclin D1; D014520:Urethane; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2