[Identification of Junci Medulla using DNA barcoding technique].
OBJECTIVE
To identify Junci Medulla using the ITS2 barcode.


METHODS
The ITS2 regions of Juncus effuses and its closely related species were PCR amplified and sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. The Kimura 2-Parameter (K2P) distances were calculated using software MEGA 5.0. Identification analyses were performed using BLAST1, Nearest Distance, PWG Distance and neighbor-joining (NJ) methods.


RESULTS
The intra-specific genetic distances of J. effuses were ranged from 0 to 0.005, which were far lower than inter-specific genetic distances between J. effuses and its closely related species (0.215-0.614). All the four methods showed that ITS2 could discriminate J. effuses from its closely related species correctly.


CONCLUSIONS
The ITS2 region is an efficient barcode for authentication of Junci Medulla, and our study further confirmed the ability of ITS2 to identify traditional Chinese medicinal materials.
The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. xhpang@implad.ac.cn
Xiaohui
X
Pang
Jingyuan
J
Song
Shilin
S
Chen
D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*
D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer