Molecular cloning of prophenoloxidase and the effects of dietary β-glucan and rutin on immune response in hemocytes of the fleshy shrimp, Fenneropenaeus chinensis.
To investigate the effects of dietary β-glucan (0.5 or 1 g kg⁻¹ diet: 0.5-BG, 1-BG) and rutin (0.5 or 1 g kg⁻¹ diet: 0.5-RT, 1-RT) after 10 days in the absence of pathogen challenge on the immune response of Fenneropenaeus chinensis, we determined total hemocyte count (THC) and the expression of four immune-related genes in hemocytes: those for prophenoloxidase (proPO), peroxinectin (PX), lipopolysaccharide and/or β-glucan binding protein (LGBP), and c-type lectin (CL). As a prerequisite for subsequent experiments, cDNA encoding proPO of the fleshy shrimp, Fenneropenaeus chinensis (f-proPO) was obtained from hemocytes; it had a full length of 3023 bp, with an open reading frame (ORF) of 2061bp, a 105-bp 5'-untranslated region, and a 906-bp 3'-untranslated region containing the poly A signal. The THCs of shrimp fed β-glucan of 1 g kg⁻¹ diet, and rutin of 1 g kg⁻¹ diet were significantly higher than that of the control (P < 0.05). The expression of proPO mRNA was slightly downregulated and that of LGBP mRNA was upregulated (except in 1-RT). PX and CL mRNA remained constitutively expressed in all groups. Our results reveal that β-glucan and rutin dietary supplements have minimal effect on immune response in the absence of pathogen challenge.
Department of Global Agricultural Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Sun-Hye
SH
Bae
Bong-Rae
BR
Kim
Bong Jung
BJ
Kang
Naoaki
N
Tsutsui
Tomoyuki
T
Okutsu
Junpei
J
Shinji
In Kwon
IK
Jang
Chang-Hee
CH
Han
Marcy N
MN
Wilder
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001774:Blood Chemical Analysis; D002352:Carrier Proteins / Q000378:metabolism; D004156:Catechol Oxidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D019587:Dietary Supplements; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006434:Hemocytes / Q000201:enzymology; D007113:Immunity, Innate; D037181:Lectins, C-Type / Q000378:metabolism; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012431:Rutin / Q000008:administration & dosage*; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D016415:Sequence Alignment; D047071:beta-Glucans / Q000008:administration & dosage*
D060829:Arthropod Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; D004792:Enzyme Precursors; D037181:Lectins, C-Type; D012333:RNA, Messenger; D047071:beta-Glucans; D012431:Rutin; C038451:pro-phenoloxidase; D004156:Catechol Oxidase