A simple microfluidic integrated with an optical sensor for micro flow injection colorimetric determination of glutathione.
A simple and inexpensive method for fabricating a microfluidic platform was developed. A printed circuit board (PCB) was used to make a master mold for replicating a polydimethylsiloxane (PDMS) microchannel. The master mold was fabricated by a simple photolithographic method, employing a photoresist dry film. The process did not use hazardous chemicals, a clean room or any expensive instrument. The PDMS microchannel was clamped with polymethylmethacrylate (PMMA) plates, where a light emitting diode (LED) as a light source and a light dependent resistor (LDR) as a light sensor were attached to form a simple optical sensor. The system was successfully employed as a micro flow injection analysis for the determination of glutathione in dietary supplement samples. A linear calibration graph in the range of 5.0 - 60.0 mg L(-1) glutathione was obtained with a detection limit of 0.01 mg L(-1). The system provided a sample throughput of 48 h(-1), with microliter consumption of the reagent.
Department of Chemistry, and Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
Sam-ang
SA
Supharoek
Napaporn
N
Youngvises
Jaroon
J
Jakmunee
D003124:Colorimetry / Q000191:economics / Q000295:instrumentation*; D003300:Copper / Q000737:chemistry; D003362:Cost-Benefit Analysis; D019587:Dietary Supplements / Q000032:analysis; D055615:Electrical Equipment and Supplies; D017022:Flow Injection Analysis / Q000191:economics / Q000295:instrumentation*; D005978:Glutathione / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D055620:Optical Phenomena; D018511:Systems Integration
D003300:Copper; D005978:Glutathione