Investigation of glucosinolate profile and qualitative aspects in sprouts and roots of horseradish (Armoracia rusticana) using LC-ESI-hybrid linear ion trap with Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation.
Within the family of Brassicaceae, an important source of glucosinolates (GLSs) is represented by horseradish ( Armoracia rusticana P. Gaertner, B. Meyer & Scherbius), cultivated for its roots, which are grated fresh or processed into a sauce and used as a condiment. The characteristic pungent flavor of the root depends on the abundance of the bioactive GLS molecules. In crude plant extracts (sprouts and roots) of an accession of horseradish largely diffused in the Basilicata region (southern Italy), which develops many sprouts and produces white, fiery, and sharp-flavored marketable roots, we characterized the GLS profile by LC-ESI-LTQ-FTICR-MS and IRMPD. In sprouts and roots we identified 16 and 11 GLSs, respectively. We confirmed the presence of sinigrin, 4-hydroxyglucobrassicin, glucobrassicin, gluconasturtin, and 4-methoxyglucobrassicin and identified glucoiberin, gluconapin, glucocochlearin, glucoconringianin, glucosativin, glucoibarin, 5-hydroxyglucobrassicin, glucocapparilinearisin or glucobrassicanapin, glucotropaeolin, and glucoarabishirsutain, not previously characterized in horseradish. Of particular note was the presence of the putative 2-methylsulfonyl-oxo-ethyl-GLS.
Ph.D. Programme "Crop Systems, Forestry and Environmental Sciences" and ‡Department of Crop Systems, Forestry and Environmental Sciences, University of Basilicata , Via dell'Ateneo Lucano, 85100 Potenza, Italy.
Rosa
R
Agneta
Anna Rita
AR
Rivelli
Emanuela
E
Ventrella
Filomena
F
Lelario
Giulio
G
Sarli
Sabino Aurelio
SA
Bufo
D031215:Armoracia / Q000737:chemistry*; D002853:Chromatography, Liquid; D017357:Cyclotrons; D005583:Fourier Analysis; D005961:Glucosinolates / Q000032:analysis*; D007211:Indoles / Q000032:analysis; D007558:Italy; D013058:Mass Spectrometry; D018517:Plant Roots / Q000737:chemistry*; D013464:Sulfuric Acids / Q000032:analysis
C552436:3-butenyl glucosinolate; C428965:4-hydroxyindol-3-ylmethylglucosinolate; D005961:Glucosinolates; D007211:Indoles; D013464:Sulfuric Acids; C513839:glucocochlearin; C403468:methoxyglucobrassicin; C457341:glucoiberin; C010330:sinigrin; C048308:glucobrassicin