Co-producing iturin A and poly-γ-glutamic acid from rapeseed meal under solid state fermentation by the newly isolated Bacillus subtilis strain 3-10.
The strain 3-10 was isolated from soil and identified as B. subtilis according to morphological and physiological characteristics and nucleotide sequence of 16S rRNA. It co-produced anti-fungal iturin A and fertilizer synergist of poly-γ-glutamic acid (γ-PGA) under solid state fermentation (SSF) with rapeseed meal. The co-production of iturin A and γ-PGA reached 5.3 and 51.3 g/kg-dry weight culture, respectively, and the number of viable cells reached 1.9 × 10(10) CFU/g-dry weight culture. In pot tests, the shoot length and dry weight of watermelon seedlings treated by the SSF culture improved by 48.0 and 30.8%, respectively compared to the control; and its biocontrol effect on watermelon fusarium wilt achieved 89.6%. These results highlight a novel strategy to exploit the low-cost and widely available rapeseed meal as dual-functional bio-organic fertilizer under SSF by B. subtilis.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Dehui
D
Yao
Zhixia
Z
Ji
Changjun
C
Wang
Gaofu
G
Qi
Lili
L
Zhang
Xin
X
Ma
Shouwen
S
Chen
D001412:Bacillus subtilis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D018533:Biomass; D029741:Brassica rapa / Q000378:metabolism*; D029773:Citrullus / Q000254:growth & development / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005285:Fermentation; D010456:Peptides, Cyclic / Q000378:metabolism*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D036226:Seedlings / Q000254:growth & development; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010456:Peptides, Cyclic; D012336:RNA, Ribosomal, 16S; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; C013579:iturin A