Influence of ShuJinHuoXue tablets on ischemia reperfusion injury of animals' skeletal muscle.
Ischemia-reperfusion (IR) can lead to serious tissue oxidative injury in animals. ShuJinHuoXue tablet (SJHXT) is a Chinese Traditional Medicine which can relax the muscles and stimulate the blood circulation and has been used as a clinical medicine. In the present study, we investigated the effects of SJHXT pretreatment on oxidative injury using an animal model of acute limb IR. Results showed that SJHXT pre-treatment (200, 300 and 400 mg/kg/day) markedly reduced serum endothelin-1 (ET-1), thromboxane B2 (TXB₂) levels and thromboxane B2/6-keto- prostaglandin F1α (TXB₂/6-Keto-PGF(1α)), wet weight/dried weight (W/D) ratio, myeloperoxidase (MPO), creatine kinase (CK), lactate dehydrogenase (LDH) activities, and increased serum nitric oxide (NO), 6-Keto-PGF(1α) levels and NO/ET-1 ratio in the IR+SJHXT groups. In addition, the SJHXT pre-treatment (200, 300 and 400 mg/kg/day) markedly reduced skeletal muscle Ca²⁺, malondialdehyde (MDA) levels, increased Na⁺-K⁺-ATPase, Ca²⁺-Mg²⁺-ATPase, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities. Our results suggest that SJHXT pre-treatment may improve skeletal muscle blood vessel microcirculation, decrease skeletal muscle oxidative injury and enhance antioxidant enzymes activities in IR animals.
Hands and Feet Microsurgery, Dalian Municipal Central Hospital, Dalian 116033, China.
Zhihong
Z
Tong
Fang
F
Yu
Zhonghua
Z
Liu
Haidong
H
Liang
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003402:Creatine Kinase / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use*; D019332:Endothelin-1 / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008315:Malondialdehyde / Q000378:metabolism; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D009569:Nitric Oxide / Q000097:blood; D009195:Peroxidase / Q000097:blood; D008517:Phytotherapy; D011460:Prostaglandins F / Q000097:blood; D011817:Rabbits; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013607:Tablets; D013929:Thromboxane B2 / Q000097:blood
D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; D011460:Prostaglandins F; D013607:Tablets; D009569:Nitric Oxide; D008315:Malondialdehyde; D013929:Thromboxane B2; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D003402:Creatine Kinase; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; C100006:prostaglandin F1